Rps27a cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Rps27a
Entrez Gene ID	100912032
Full Name	ribosomal protein S27a
General protein information	Preferred Names ribosomal protein S27a Names ubiquitin-40S ribosomal protein S27a ubiquitin carboxyl extension protein 80
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 14 Map Location: 14q22
Summary	The protein encoded by this gene is a fusion protein that contains ubiquitin at its N-terminus and ribosomal protein S27a at its C-terminus. When the human ortholog of this protein is expressed in yeast, it is processed post-translationally into two products, a free ubiquitin monomer and ribosomal protein S27a, a component of the 40S ribosomal subunit. There are multiple pseudogenes of this gene. There is a locus on chromosome 5 (GeneID:81777) that contains an intact copy of the open reading frame of this gene, that is likely to be the result of retrotransposition of an mRNA into the genome. The transcriptional status of this locus cannot be verified at the present time. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2015].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001305443.1	NP_001292372.1	ubiquitin-40S ribosomal protein S27a
NM_031113.2	NP_112375.1	ubiquitin-40S ribosomal protein S27a

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	RPS27A	NP_001239157.1
Mus musculus (house mouse)	Rps27a	NP_001029037.1
Saccharomyces cerevisiae (baker's yeast)	RPS31	NP_013268.1
Magnaporthe oryzae (rice blast fungus)	MGG_07928	XP_003713173.1
Pan troglodytes (chimpanzee)	LOC101059860	XP_003949845.1
Eremothecium gossypii	AGOS_AGL128W	NP_986539.1
Arabidopsis thaliana (thale cress)	AT1G23410	NP_173755.1
Homo sapiens (human)	RPS27A	NP_001129064.1
Bos taurus (cattle)	RPS27A	NP_777203.1
Rattus norvegicus (Norway rat)	Rps27a	NP_112375.1
Rattus norvegicus (Norway rat)	LOC100912032	XP_003751469.1
Danio rerio (zebrafish)	rps27a	NP_956796.1
Kluyveromyces lactis	KLLA0D18304g	XP_453871.1
Schizosaccharomyces pombe (fission yeast)	ubi3	NP_594108.1
Neurospora crassa	NCU04553	XP_955944.2
Arabidopsis thaliana (thale cress)	UBQ5	NP_191784.1
Arabidopsis thaliana (thale cress)	UBQ6	NP_566095.1
Oryza sativa (rice)	Os05g0160200	NP_001054720.2
	RPS27A	XP_001112274.1
Pan troglodytes (chimpanzee)	RPS27A	NP_001239528.1
Gallus gallus (chicken)	RPS27A	NP_990284.1
Drosophila melanogaster (fruit fly)	RpS27A	NP_476778.1
Schizosaccharomyces pombe (fission yeast)	SPAC589.10c	NP_594058.1
Oryza sativa (rice)	Os01g0328400	NP_001042915.1
Xenopus tropicalis (tropical clawed frog)	rps27a	NP_001016172.1

Gene Ontology
Bibliography

Related articles in PubMed

Structures of the human and Drosophila 80S ribosome.
Anger AM, Armache JP, Berninghausen O, Habeck M, Subklewe M, Wilson DN, Beckmann R
Nature497(7447)80-5(2013 May)

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Castello A, Fischer B, Eichelbaum K, Horos R, Beckmann BM, Strein C, Davey NE, Humphreys DT, Preiss T, Steinmetz LM, Krijgsveld J, Hentze MW
Cell149(6)1393-406(2012 Jun)

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Baltz AG, Munschauer M, Schwanh?usser B, Vasile A, Murakawa Y, Schueler M, Youngs N, Penfold-Brown D, Drew K, Milek M, Wyler E, Bonneau R, Selbach M, Dieterich C, Landthaler M
Molecular cell46(5)674-90(2012 Jun)

Proteomic characterization of the human sperm nucleus.
de Mateo S, Castillo J, Estanyol JM, Ballesc? JL, Oliva R
Proteomics11(13)2714-26(2011 Jul)

Functional dichotomy of ribosomal proteins during the synthesis of mammalian 40S ribosomal subunits.
O'Donohue MF, Choesmel V, Faubladier M, Fichant G, Gleizes PE
The Journal of cell biology190(5)853-66(2010 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Rps27a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Rps27a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa20086	NM_001305443.1 Latest version!	Rattus norvegicus ribosomal protein S27a (Rps27a), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
ORa20086	NM_031113.2 Latest version!	Rattus norvegicus ribosomal protein S27a (Rps27a), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Rps27a ( NM_001305443.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001292372.1 Rattus norvegicus ribosomal protein S27a (Rps27a), transcript variant 2, mRNA.

Price & Availability↑

CloneID	ORa20086
Clone ID Related Accession (Same CDS sequence)	NM_001305443.1 , NM_031113.2
Accession Version	NM_001305443.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 471bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-23
Organism	Rattus norvegicus(Norway rat)
Product	ubiquitin-40S ribosomal protein S27a
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AABR07016777.1 and FM074661.1. Transcript Variant: This variant (2) differs in the 5' UTR, compared to variant 1. Both variants 1 and 2 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript from the same strain was available for the full length of the gene. The extent of this transcript is supported by transcript alignments and orthologous data. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: CF110071.1, FM072030.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCAGATTT TTGTGAAGAC CCTTACAGGG AAGACCATCA CGCTCGAGGT TGAACCCTCG 
GACACTATAG AAAATGTAAA GGCCAAGATC CAGGATAAGG AAGGAATTCC TCCTGATCAG 
CAGAGGTTGA TCTTTGCTGG TAAGCAGTTG GAAGATGGCC GTACTTTGTC TGACTACAAC 
ATTCAAAAGG AGTCCACTCT CCATCTCGTG CTGAGACTTC GTGGTGGCGC TAAGAAAAGG 
AAGAAGAAGT CTTACACCAC CCCAAAGAAG AACAAGCATA AGAGAAAGAA GGTCAAGTTG 
GCTGTGCTGA AATACTATAA GGTGGATGAA AATGGCAAAA TCAGCCGACT TCGTCGGGAA 
TGTCCTTCTG ATGAATGTGG TGCTGGAGTT TTCATGGGTA GCCACTTTGA CAGGCATTAC 
TGTGGCAAGT GTTGTCTGAC TTACTGCTTC AACAAACCAG AAGACAAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001292372.1
CDS	117..587
Translation	MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGAKKRKKKSYTTPKKNKHKRKKVKLAVLKYYKVDENGKISRLRRECPSDECGAGVFMGSHFDRHYCGKCCLTYCFNKPEDK

Target ORF information:

RefSeq Version	NM_001305443.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ribosomal protein S27a (Rps27a), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001305443.1

ORF Insert Sequence:

ATGCAGATTT TTGTGAAGAC CCTTACAGGG AAGACCATCA CGCTCGAGGT TGAACCCTCG 
GACACTATAG AAAATGTAAA GGCCAAGATC CAGGATAAGG AAGGAATTCC TCCTGATCAG 
CAGAGGTTGA TCTTTGCTGG TAAGCAGTTG GAAGATGGCC GTACTTTGTC TGACTACAAC 
ATTCAAAAGG AGTCCACTCT CCATCTCGTG CTGAGACTTC GTGGTGGCGC TAAGAAAAGG 
AAGAAGAAGT CTTACACCAC CCCAAAGAAG AACAAGCATA AGAGAAAGAA GGTCAAGTTG 
GCTGTGCTGA AATACTATAA GGTGGATGAA AATGGCAAAA TCAGCCGACT TCGTCGGGAA 
TGTCCTTCTG ATGAATGTGG TGCTGGAGTT TTCATGGGTA GCCACTTTGA CAGGCATTAC 
TGTGGCAAGT GTTGTCTGAC TTACTGCTTC AACAAACCAG AAGACAAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rps27a ( NM_031113.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_112375.1 Rattus norvegicus ribosomal protein S27a (Rps27a), transcript variant 1, mRNA.

Price & Availability↑

CloneID	ORa20086
Clone ID Related Accession (Same CDS sequence)	NM_001305443.1 , NM_031113.2
Accession Version	NM_031113.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 471bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-23
Organism	Rattus norvegicus(Norway rat)
Product	ubiquitin-40S ribosomal protein S27a
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AABR07016777.1 and FM074661.1. On or before Mar 13, 2015 this sequence version replaced XM_003751421.3, XM_006221879.2, NM_031113.1. Transcript Variant: This variant (1) represents the shorter transcript. Both variants 1 and 2 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript from the same strain was available for the full length of the gene. The extent of this transcript is supported by transcript alignments and orthologous data. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: FQ220688.1, CF976553.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCAGATTT TTGTGAAGAC CCTTACAGGG AAGACCATCA CGCTCGAGGT TGAACCCTCG 
GACACTATAG AAAATGTAAA GGCCAAGATC CAGGATAAGG AAGGAATTCC TCCTGATCAG 
CAGAGGTTGA TCTTTGCTGG TAAGCAGTTG GAAGATGGCC GTACTTTGTC TGACTACAAC 
ATTCAAAAGG AGTCCACTCT CCATCTCGTG CTGAGACTTC GTGGTGGCGC TAAGAAAAGG 
AAGAAGAAGT CTTACACCAC CCCAAAGAAG AACAAGCATA AGAGAAAGAA GGTCAAGTTG 
GCTGTGCTGA AATACTATAA GGTGGATGAA AATGGCAAAA TCAGCCGACT TCGTCGGGAA 
TGTCCTTCTG ATGAATGTGG TGCTGGAGTT TTCATGGGTA GCCACTTTGA CAGGCATTAC 
TGTGGCAAGT GTTGTCTGAC TTACTGCTTC AACAAACCAG AAGACAAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_112375.1
CDS	78..548
Translation	MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGAKKRKKKSYTTPKKNKHKRKKVKLAVLKYYKVDENGKISRLRRECPSDECGAGVFMGSHFDRHYCGKCCLTYCFNKPEDK

Target ORF information:

RefSeq Version	NM_031113.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ribosomal protein S27a (Rps27a), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_031113.2

ORF Insert Sequence:

ATGCAGATTT TTGTGAAGAC CCTTACAGGG AAGACCATCA CGCTCGAGGT TGAACCCTCG 
GACACTATAG AAAATGTAAA GGCCAAGATC CAGGATAAGG AAGGAATTCC TCCTGATCAG 
CAGAGGTTGA TCTTTGCTGG TAAGCAGTTG GAAGATGGCC GTACTTTGTC TGACTACAAC 
ATTCAAAAGG AGTCCACTCT CCATCTCGTG CTGAGACTTC GTGGTGGCGC TAAGAAAAGG 
AAGAAGAAGT CTTACACCAC CCCAAAGAAG AACAAGCATA AGAGAAAGAA GGTCAAGTTG 
GCTGTGCTGA AATACTATAA GGTGGATGAA AATGGCAAAA TCAGCCGACT TCGTCGGGAA 
TGTCCTTCTG ATGAATGTGG TGCTGGAGTT TTCATGGGTA GCCACTTTGA CAGGCATTAC 
TGTGGCAAGT GTTGTCTGAC TTACTGCTTC AACAAACCAG AAGACAAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Structures of the human and Drosophila 80S ribosome.
Nature497(7447)80-5(2013 May)
Anger AM,Armache JP,Berninghausen O,Habeck M,Subklewe M,Wilson DN,Beckmann R

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Cell149(6)1393-406(2012 Jun)
Castello A,Fischer B,Eichelbaum K,Horos R,Beckmann BM,Strein C,Davey NE,Humphreys DT,Preiss T,Steinmetz LM,Krijgsveld J,Hentze MW

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Molecular cell46(5)674-90(2012 Jun)
Baltz AG,Munschauer M,Schwanh?usser B,Vasile A,Murakawa Y,Schueler M,Youngs N,Penfold-Brown D,Drew K,Milek M,Wyler E,Bonneau R,Selbach M,Dieterich C,Landthaler M

Proteomic characterization of the human sperm nucleus.
Proteomics11(13)2714-26(2011 Jul)
de Mateo S,Castillo J,Estanyol JM,Ballesc? JL,Oliva R

Functional dichotomy of ribosomal proteins during the synthesis of mammalian 40S ribosomal subunits.
The Journal of cell biology190(5)853-66(2010 Sep)
O'Donohue MF,Choesmel V,Faubladier M,Fichant G,Gleizes PE

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Rps27a cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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