Crnkl1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Crnkl1
Entrez Gene ID	100910202
Full Name	crooked neck pre-mRNA splicing factor 1
Synonyms	Crn
General protein information	Preferred Names crooked neck pre-mRNA splicing factor 1 Names crooked neck-like protein 1 Crn, crooked neck-like 1 crooked neck homolog crooked neck pre-mRNA splicing factor-like 1 crooked neck-like 1 protein-like crooked neck-like protein 1-like
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 6 Map Location: 6q12
Summary	drosophila homolog may participate in the assembly of the splicing machinery [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_053797.2	NP_446249.1	crooked neck-like protein 1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	CRNKL1	NP_001193451.1
Rattus norvegicus (Norway rat)	LOC102548514	XP_006235227.1
Arabidopsis thaliana (thale cress)	AT3G13210	NP_187927.1
Oryza sativa (rice)	Os06g0523800	NP_001174825.1
Homo sapiens (human)	CRNKL1	NP_057736.4
Pan troglodytes (chimpanzee)	CRNKL1	XP_514541.2
Gallus gallus (chicken)	CRNKL1	XP_419315.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001879	XP_321188.4
Neurospora crassa	NCU02717	XP_965102.1
Arabidopsis thaliana (thale cress)	AT5G45990	NP_199411.1
Danio rerio (zebrafish)	crnkl1	NP_957240.1
Caenorhabditis elegans (roundworm)	M03F8.3	NP_001122979.1
Schizosaccharomyces pombe (fission yeast)	cwf4	NP_596573.1
Magnaporthe oryzae (rice blast fungus)	MGG_05720	XP_003710622.1
Oryza sativa (rice)	Os05g0289400	NP_001055097.1
Xenopus tropicalis (tropical clawed frog)	crnkl1	NP_001016895.1
Arabidopsis thaliana (thale cress)	AT5G41770	NP_198992.2
	LOC699931	XP_002808010.1
Canis lupus familiaris (dog)	CRNKL1	XP_005634771.1
Mus musculus (house mouse)	Crnkl1	NP_080096.1
Rattus norvegicus (Norway rat)	Crnkl1	NP_446249.1
Drosophila melanogaster (fruit fly)	crn	NP_477118.1

Gene Ontology
Bibliography

Related articles in PubMed

Cryo-EM structure of a human spliceosome activated for step 2 of splicing.
Bertram K, Agafonov DE, Liu WT, Dybkov O, Will CL, Hartmuth K, Urlaub H, Kastner B, Stark H, L?hrmann R
Nature542(7641)318-323(2017 02)

Mutational landscape of basal cell carcinomas by whole-exome sequencing.
Jayaraman SS, Rayhan DJ, Hazany S, Kolodney MS
The Journal of investigative dermatology134(1)213-220(2014 Jan)

Splicing and beyond: the many faces of the Prp19 complex.
Chanarat S, Str??er K
Biochimica et biophysica acta1833(10)2126-34(2013 Oct)

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Baltz AG, Munschauer M, Schwanh?usser B, Vasile A, Murakawa Y, Schueler M, Youngs N, Penfold-Brown D, Drew K, Milek M, Wyler E, Bonneau R, Selbach M, Dieterich C, Landthaler M
Molecular cell46(5)674-90(2012 Jun)

A novel rat orthologue and homologue for the Drosophila crooked neck gene in neural stem cells and their immediate descendants.
Amada N, Tezuka T, Mayeda A, Araki K, Takei N, Todokoro K, Nawa H
Journal of biochemistry133(5)615-23(2003 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Crnkl1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Crnkl1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa00026	NM_053797.2 Latest version!	Rattus norvegicus crooked neck pre-mRNA splicing factor 1 (Crnkl1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$391.30 $559.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Crnkl1 ( NM_053797.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_446249.1 Rattus norvegicus crooked neck pre-mRNA splicing factor 1 (Crnkl1), mRNA.

Price & Availability↑

CloneID	ORa00026
Clone ID Related Accession (Same CDS sequence)	NM_053797.2
Accession Version	NM_053797.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2073bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-23
Organism	Rattus norvegicus(Norway rat)
Product	crooked neck-like protein 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC085718.1. On or before Apr 14, 2013 this sequence version replaced XM_003749580.1, XM_003753805.1, NM_053797.1. ##Evidence-Data-START## Transcript is intronless :: BC085718.1 [ECO:0000345] ##Evidence-Data-END##

ATGGCGGCTT CCACGGCAGC CGGGAAACAG CGGATTCCTA AAGTGGCCAA GGTGAAGAAC 
AAAGCGCCAG CAGAGGTGCA GATAACCGCA GAGCAGCTCT TAAGGGAGGC TAAAGAAAGA 
GAGCTCGAGC TCCTCCCACC CCCACCTCAG CAGAAGATCA CAGACGAGGA AGAACTAAAC 
GATTACAAAC TACGGAAAAG GAAGACTTTC GAAGATAATA TAAGAAAAAA CAGAACTGTG 
ATTAGCAACT GGATCAAATA CGCACAATGG GAGGAAAGTC TGAAGGAAAT TCAAAGGGCC 
CGATCCATAT ACGAGCGTGC TTTAGATGTC GACTACCGAA ATATCACACT CTGGCTGAAA 
TATGCAGAAA TGGAAATGAA GAACCGCCAG GTCAACCATG CCCGAAATAT CTGGGACCGT 
GCCATAACAA CCCTGCCGCG AGTCAACCAG TTCTGGTACA AATACACGTA CATGGAGGAG 
ATGCTGGGCA ATGTTGCCGG TGCTCGTCAG GTGTTTGAGC GCTGGATGGA ATGGCAGCCT 
GAGGAGCAGG CCTGGCACTC CTACATCAAC TTCGAGCTGA GATACAAAGA GGTGGAGCGG 
GCCCGCACCA TTTATGAACG CTTTGTGCTC GTGCACCCTG CTGTGAAGAA CTGGATCAAG 
TACGCTCGAT TTGAAGAGAA GCATGCGTAT TTTGCCCATG CGCGGAAAGT CTACGAGAGA 
GCAGTTGAGT TTTTTGGGGA TGAGCATATG GACGAACACC TCTATGTGGC CTTTGCTAAA 
TTTGAAGAAA ATCAAAAAGA ATTTGAAAGG GTACGAGTTA TCTACAAATA TGCCCTGGAT 
AGGATTTCCA AGCAAGAGGC CCAAGAGCTC TTTAAAAACT ATACCATCTT TGAGAAGAAG 
TTTGGTGACC GGAGGGGTAT AGAAGACATC ATCGTGAGCA AACGGAGATT CCAGTATGAA 
GAAGAAGTGA AGGCTAATCC ACACAATTAT GATGCATGGT TTGATTATTT ACGCTTGGTG 
GAAAGTGATG CAGAAGCGGA CACTGTGCGG GAAGTCTATG AGCGAGCCAT TGCCAATGTG 
CCACCCATCC AGGAGAAGAG GCACTGGAAA CGCTACATCT ACCTCTGGGT TAACTACGCA 
CTCTATGAAG AGCTGGAGGC CAAGGATCCT GAGAGGACAA GACAGGTGTA TCAAGCATCT 
TTGGAACTAA TTCCTCACAA AAAGTTCACG TTTGCCAAAA TGTGGTTATA TTACGCACAG 
TTTGAAATAA GACAGAAAAA TCTGCCATTT GCCAGAAGAG CTTTGGGAAC TTCCATAGGC 
AAATGTCCCA AGAACAAGTT ATTCAAAGGT TACATAGAAC TGGAACTGCA GCTTCGAGAA 
TTTGACAGAT GCAGGAAGCT TTACGAAAAG TTTTTGGAAT TTGGACCTGA AAATTGTACC 
TCGTGGATTA AGTTTGCAGA ATTAGAAACA ATCCTTGGTG ATATTGAGAG AGCTCGGGCA 
ATCTATGAAT TAGCCATCAG CCAGCCACGC TTGGATATGC CAGAGGTACT TTGGAAATCA 
TATATTGATT TTGAGATTGA GCAGGAAGAA ACTGAGAGAA CACGAAATCT TTACCGACAG 
TTGCTTCAGC GAACACAGCA CGTCAAGGTA TGGATCAGTT TTGCTCAGTT TGAATTATCT 
TCTGGGAAAG AAGGAAGTGT GGCTAAATGC AGACAGATTT ATGAAGAAGC TAACAAAACC 
ATGAGAAACT GTGAAGAAAA AGAAGAGAGG CTTATGTTGC TGGAGTCCTG GCGAAGTTTC 
GAAGATGAAT TTGGAACAGT ATCAGACAAG GAGAGAGTAG ACAAACTCAT GCCAGAAAAG 
GTCAAGAAGA GAAGAAAGGT CCAGGCTGAC GATGGGTCTG ATGCCGGATG GGAAGAGTAC 
TATGACTACA TCTTTCCAGA GGATGCTGCC AACCAGCCTA ACCTCAAGCT GCTGGCGATG 
GCCAAGCTTT GGAAGAAACA GCAACAAGAA AGAGAAGCTG CCGAACAGGA TCCAGATAAG 
GACATTGATG AGAGTGAATC CTCATCTTTT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_446249.1
CDS	87..2159
Translation	MAASTAAGKQRIPKVAKVKNKAPAEVQITAEQLLREAKERELELLPPPPQQKITDEEELNDYKLRKRKTFEDNIRKNRTVISNWIKYAQWEESLKEIQRARSIYERALDVDYRNITLWLKYAEMEMKNRQVNHARNIWDRAITTLPRVNQFWYKYTYMEEMLGNVAGARQVFERWMEWQPEEQAWHSYINFELRYKEVERARTIYERFVLVHPAVKNWIKYARFEEKHAYFAHARKVYERAVEFFGDEHMDEHLYVAFAKFEENQKEFERVRVIYKYALDRISKQEAQELFKNYTIFEKKFGDRRGIEDIIVSKRRFQYEEEVKANPHNYDAWFDYLRLVESDAEADTVREVYERAIANVPPIQEKRHWKRYIYLWVNYALYEELEAKDPERTRQVYQASLELIPHKKFTFAKMWLYYAQFEIRQKNLPFARRALGTSIGKCPKNKLFKGYIELELQLREFDRCRKLYEKFLEFGPENCTSWIKFAELETILGDIERARAIYELAISQPRLDMPEVLWKSYIDFEIEQEETERTRNLYRQLLQRTQHVKVWISFAQFELSSGKEGSVAKCRQIYEEANKTMRNCEEKEERLMLLESWRSFEDEFGTVSDKERVDKLMPEKVKKRRKVQADDGSDAGWEEYYDYIFPEDAANQPNLKLLAMAKLWKKQQQEREAAEQDPDKDIDESESSSF

Target ORF information:

RefSeq Version	NM_053797.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus crooked neck pre-mRNA splicing factor 1 (Crnkl1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_053797.2

ORF Insert Sequence:

ATGGCGGCTT CCACGGCAGC CGGGAAACAG CGGATTCCTA AAGTGGCCAA GGTGAAGAAC 
AAAGCGCCAG CAGAGGTGCA GATAACCGCA GAGCAGCTCT TAAGGGAGGC TAAAGAAAGA 
GAGCTCGAGC TCCTCCCACC CCCACCTCAG CAGAAGATCA CAGACGAGGA AGAACTAAAC 
GATTACAAAC TACGGAAAAG GAAGACTTTC GAAGATAATA TAAGAAAAAA CAGAACTGTG 
ATTAGCAACT GGATCAAATA CGCACAATGG GAGGAAAGTC TGAAGGAAAT TCAAAGGGCC 
CGATCCATAT ACGAGCGTGC TTTAGATGTC GACTACCGAA ATATCACACT CTGGCTGAAA 
TATGCAGAAA TGGAAATGAA GAACCGCCAG GTCAACCATG CCCGAAATAT CTGGGACCGT 
GCCATAACAA CCCTGCCGCG AGTCAACCAG TTCTGGTACA AATACACGTA CATGGAGGAG 
ATGCTGGGCA ATGTTGCCGG TGCTCGTCAG GTGTTTGAGC GCTGGATGGA ATGGCAGCCT 
GAGGAGCAGG CCTGGCACTC CTACATCAAC TTCGAGCTGA GATACAAAGA GGTGGAGCGG 
GCCCGCACCA TTTATGAACG CTTTGTGCTC GTGCACCCTG CTGTGAAGAA CTGGATCAAG 
TACGCTCGAT TTGAAGAGAA GCATGCGTAT TTTGCCCATG CGCGGAAAGT CTACGAGAGA 
GCAGTTGAGT TTTTTGGGGA TGAGCATATG GACGAACACC TCTATGTGGC CTTTGCTAAA 
TTTGAAGAAA ATCAAAAAGA ATTTGAAAGG GTACGAGTTA TCTACAAATA TGCCCTGGAT 
AGGATTTCCA AGCAAGAGGC CCAAGAGCTC TTTAAAAACT ATACCATCTT TGAGAAGAAG 
TTTGGTGACC GGAGGGGTAT AGAAGACATC ATCGTGAGCA AACGGAGATT CCAGTATGAA 
GAAGAAGTGA AGGCTAATCC ACACAATTAT GATGCATGGT TTGATTATTT ACGCTTGGTG 
GAAAGTGATG CAGAAGCGGA CACTGTGCGG GAAGTCTATG AGCGAGCCAT TGCCAATGTG 
CCACCCATCC AGGAGAAGAG GCACTGGAAA CGCTACATCT ACCTCTGGGT TAACTACGCA 
CTCTATGAAG AGCTGGAGGC CAAGGATCCT GAGAGGACAA GACAGGTGTA TCAAGCATCT 
TTGGAACTAA TTCCTCACAA AAAGTTCACG TTTGCCAAAA TGTGGTTATA TTACGCACAG 
TTTGAAATAA GACAGAAAAA TCTGCCATTT GCCAGAAGAG CTTTGGGAAC TTCCATAGGC 
AAATGTCCCA AGAACAAGTT ATTCAAAGGT TACATAGAAC TGGAACTGCA GCTTCGAGAA 
TTTGACAGAT GCAGGAAGCT TTACGAAAAG TTTTTGGAAT TTGGACCTGA AAATTGTACC 
TCGTGGATTA AGTTTGCAGA ATTAGAAACA ATCCTTGGTG ATATTGAGAG AGCTCGGGCA 
ATCTATGAAT TAGCCATCAG CCAGCCACGC TTGGATATGC CAGAGGTACT TTGGAAATCA 
TATATTGATT TTGAGATTGA GCAGGAAGAA ACTGAGAGAA CACGAAATCT TTACCGACAG 
TTGCTTCAGC GAACACAGCA CGTCAAGGTA TGGATCAGTT TTGCTCAGTT TGAATTATCT 
TCTGGGAAAG AAGGAAGTGT GGCTAAATGC AGACAGATTT ATGAAGAAGC TAACAAAACC 
ATGAGAAACT GTGAAGAAAA AGAAGAGAGG CTTATGTTGC TGGAGTCCTG GCGAAGTTTC 
GAAGATGAAT TTGGAACAGT ATCAGACAAG GAGAGAGTAG ACAAACTCAT GCCAGAAAAG 
GTCAAGAAGA GAAGAAAGGT CCAGGCTGAC GATGGGTCTG ATGCCGGATG GGAAGAGTAC 
TATGACTACA TCTTTCCAGA GGATGCTGCC AACCAGCCTA ACCTCAAGCT GCTGGCGATG 
GCCAAGCTTT GGAAGAAACA GCAACAAGAA AGAGAAGCTG CCGAACAGGA TCCAGATAAG 
GACATTGATG AGAGTGAATC CTCATCTTTT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Cryo-EM structure of a human spliceosome activated for step 2 of splicing.
Nature542(7641)318-323(2017 02)
Bertram K,Agafonov DE,Liu WT,Dybkov O,Will CL,Hartmuth K,Urlaub H,Kastner B,Stark H,L?hrmann R

Mutational landscape of basal cell carcinomas by whole-exome sequencing.
The Journal of investigative dermatology134(1)213-220(2014 Jan)
Jayaraman SS,Rayhan DJ,Hazany S,Kolodney MS

Splicing and beyond: the many faces of the Prp19 complex.
Biochimica et biophysica acta1833(10)2126-34(2013 Oct)
Chanarat S,Str??er K

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Molecular cell46(5)674-90(2012 Jun)
Baltz AG,Munschauer M,Schwanh?usser B,Vasile A,Murakawa Y,Schueler M,Youngs N,Penfold-Brown D,Drew K,Milek M,Wyler E,Bonneau R,Selbach M,Dieterich C,Landthaler M

A novel rat orthologue and homologue for the Drosophila crooked neck gene in neural stem cells and their immediate descendants.
Journal of biochemistry133(5)615-23(2003 May)
Amada N,Tezuka T,Mayeda A,Araki K,Takei N,Todokoro K,Nawa H

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Crnkl1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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