Tbcd cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Tbcd
Entrez Gene ID	100361417
Full Name	tubulin folding cofactor D
General protein information	Preferred Names tubulin folding cofactor D Names tubulin-specific chaperone D
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 10 Map Location: 10q32.3

mRNA and Protein(s)

mRNA	Protein	Name
NM_001271364.1	NP_001258293.1	tubulin-specific chaperone D
XM_008768501.2	XP_008766723.1	tubulin-specific chaperone D isoform X3
XM_006247861.3	XP_006247923.1	tubulin-specific chaperone D isoform X1
XM_006247864.3	XP_006247926.1	tubulin-specific chaperone D isoform X2
XM_008768502.2	XP_008766724.1	tubulin-specific chaperone D isoform X3

Pathways from BioSystems

Homologies

	LOC719682	XP_002800701.1
Xenopus tropicalis (tropical clawed frog)	tbcd	NP_001072379.1
Bos taurus (cattle)	TBCD	NP_776619.1
Rattus norvegicus (Norway rat)	Tbcd	NP_001258293.1
Danio rerio (zebrafish)	tbcd	XP_001922584.2
Pan troglodytes (chimpanzee)	TBCD	XP_001168814.2
Homo sapiens (human)	TBCD	NP_005984.3
Drosophila melanogaster (fruit fly)	CG7261	NP_608648.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004782	XP_318031.4
Arabidopsis thaliana (thale cress)	TTN1	NP_191633.3
Canis lupus familiaris (dog)	TBCD	XP_548804.4
Mus musculus (house mouse)	Tbcd	NP_084154.1
Gallus gallus (chicken)	TBCD	NP_001012819.1
Oryza sativa (rice)	Os10g0508500	NP_001065023.1

Gene Ontology
Bibliography

Related articles in PubMed

Infantile neurodegenerative disorder associated with mutations in TBCD, an essential gene in the tubulin heterodimer assembly pathway.
Edvardson S, Tian G, Cullen H, Vanyai H, Ngo L, Bhat S, Aran A, Daana M, Da'amseh N, Abu-Libdeh B, Cowan NJ, Heng JI, Elpeleg O
Human molecular genetics25(21)4635-4648(2016 11)

Biallelic Mutations in TBCD, Encoding the Tubulin Folding Cofactor D, Perturb Microtubule Dynamics and Cause Early-Onset Encephalopathy.
Flex E, Niceta M, Cecchetti S, Thiffault I, Au MG, Capuano A, Piermarini E, Ivanova AA, Francis JW, Chillemi G, Chandramouli B, Carpentieri G, Haaxma CA, Ciolfi A, Pizzi S, Douglas GV, Levine K, Sferra A, Dentici ML, Pfundt RR, Le Pichon JB, Farrow E, Baas F, Piemonte F, Dallapiccola B, Graham JM Jr, Saunders CJ, Bertini E, Kahn RA, Koolen DA, Tartaglia M
American journal of human genetics99(4)962-973(2016 Oct)

Biallelic TBCD Mutations Cause Early-Onset Neurodegenerative Encephalopathy.
Miyake N, Fukai R, Ohba C, Chihara T, Miura M, Shimizu H, Kakita A, Imagawa E, Shiina M, Ogata K, Okuno-Yuguchi J, Fueki N, Ogiso Y, Suzumura H, Watabe Y, Imataka G, Leong HY, Fattal-Valevski A, Kramer U, Miyatake S, Kato M, Okamoto N, Sato Y, Mitsuhashi S, Nishino I, Kaneko N, Nishiyama A, Tamura T, Mizuguchi T, Nakashima M, Tanaka F, Saitsu H, Matsumoto N
American journal of human genetics99(4)950-961(2016 Oct)

Effect of TBCD and its regulatory interactor Arl2 on tubulin and microtubule integrity.
Tian G, Thomas S, Cowan NJ
Cytoskeleton (Hoboken, N.J.)67(11)706-14(2010 Nov)

Gene and alternative splicing annotation with AIR.
Florea L, Di Francesco V, Miller J, Turner R, Yao A, Harris M, Walenz B, Mobarry C, Merkulov GV, Charlab R, Dew I, Deng Z, Istrail S, Li P, Sutton G
Genome research15(1)54-66(2005 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Tbcd gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Tbcd cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa50817	XM_008768501.2 Latest version!	Rattus norvegicus tubulin folding cofactor D (Tbcd), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
ORa50817	XM_008768502.2 Latest version!	Rattus norvegicus tubulin folding cofactor D (Tbcd), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
ORa11083	NM_001271364.1 Latest version!	Rattus norvegicus tubulin folding cofactor D (Tbcd), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
ORa34010	XM_006247861.3 Latest version!	Rattus norvegicus tubulin folding cofactor D (Tbcd), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
ORa34013	XM_006247864.3 Latest version!	Rattus norvegicus tubulin folding cofactor D (Tbcd), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Tbcd ( XM_008768501.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008766723.1 Rattus norvegicus tubulin folding cofactor D (Tbcd), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa50817
Clone ID Related Accession (Same CDS sequence)	XM_008768501.2 , XM_008768502.2
Accession Version	XM_008768501.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2967bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	tubulin-specific chaperone D isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008768501.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGCT TTCTGGACTG GAGCCTTTGT ACCTTGGCCC ACTCTTCCTT CCAGACCATT 
GACGGTGTCA TTACCATGGA TGGGATGTTG CAGGCCCTGG CTCAAATATT TAAGCATGGA 
AAACGTGAAG ACTGTCTACC CTATGCCAAC ACTGTGCTCC AGTGCCTTGA TGGATGTAGA 
CTCCCTGAGA GCAGTCACAC CTTACTGCGG AAGCTTGGTG TGAAGCTTGT CCAGAGGCTG 
GGGCTGACTT TCCTGAAGCC AAAGGTGGCC ACGTGGAGGT ATCAGCGTGG CTGTCGGTCT 
TTGGCTGCCA ACCTGCAGCT CCGTGCTCCG GGTAAGAGTG AACAGAAGCT GCTGTCTGAT 
AGTTTGACCT CTGATGGTGA TGAAGACTAT GATGTCCCAG AGGGGGTGGA AACTGTGATA 
GAGCAACTGC TGGTTGGGCT GAAGGACAAG GACACGATAG TGCGGTGGTC TGCAGCTAAA 
GGAATTGGTA GGATGGCTGG AAGGCTTCCC AGAGAGCTGG CAGATGATGT GGTGGGGTCC 
GTGTTGGACT GTTTCAGCTT TCAGGAGACA GATAAGGCAT GGCATGGTGG ATGTCTGGCA 
CTGGCAGAGC TGGGTAGACG AGGCCTGTTG CTGCCATCGA GACTCTCAGA GGTTGTCACA 
GTGATCCTGA AGGCCCTTAC CTACGATGAG AAGCGGGGTG CCTGCAGCGT GGGGGCCAAC 
GTCAGGGATG CTGCCTGCTA TGTGTGCTGG GCTTTTGCAC GTGCCTACGA GCCTCAGGAG 
TTGACACCTT TCGTGACTGC CATTTCAAGT GCCCTGGTCA TTGCTGCTGT ATTTGACCGA 
AACGTGAACT GCAGAAGAGC AGCCTCTGCT GCCTTCCAGG AGAACGTGGG GAGACAGGGT 
ACTTTTCCTC ATGGAATTGA TATTTTGACT ACAGCAGACT ATTTTGCAGT TGGTAACATT 
TCTAACTGTT TCCTGATTAT AAGCGTGTTT ATTGCTGGCT TCCAGGAATA CACCAAGCCA 
ATGATCGACC ACCTGGTCTC CATGAAAATC AACCACTGGG ATGGGGCCAT CCGAGAACTG 
TCTGCAAAGG CACTGCACAA CCTGACTTCA CTAGTACCCG AGTACATGGC CACTCACGTA 
TTCCCAGCAC TACTGCTGAT GACACAGAGT CCAGATCTAC ACACGAGACA TGGTGCCATC 
CTTGCCTGTG CAGAGGTCAC CTATGCCCTG TACGAGCTGG CCGCACAGAG TAACAGGCTT 
GTCACAGACT ATCTGGATGA GAAGGCAGTG CAAAGCCTGA AGCAGATCCA CCAGCAGCTT 
TGTGATCGCC ATCTCTACCG GGGTCTGGGA GGAGAGCTTA TGAGACAAGC AGTGTGCATC 
TTAATAGAGA AATTGTCACT TTCCAGAATG CCCTTTAAAG GTGACACCGT TATTGAGGGA 
TGGCAGTGGC TGATAAATGA CACTCTCAGG AGCCTTCACC TTGTTTCAAG CCATTCCAGA 
GAACAGATCA AGGAGGTGGC TGTCTCTGCC CTGGCTGCCG TCTGCAATGA ATACTACAAG 
AAGGAGCCTG GGGAGGCAGG CTCTACCATC ACAGAGGAGC TGATTCCACA GTACCTTGCT 
GAACTCCGAA GCCCAGAGGA GATGACCCGT TGTGGCTTTT CCTCAGCCTT GGGTGCCCTC 
CCGTGTTTCC TTCTCAGGGG CCATCTGCAG CAGGTTCTCT CAGGTCTGAG AAGAGTCACC 
TGTACTACTT CTCCCATGGA TGTGAGCTTT GCTGAGGCCA GGAGAGACGG TTTGAAAGCA 
ATCTCGAGAA TTTGCCAGAC AGTTGGTGTG AACACACAGG GACCTCCAGA TGAAGTCATA 
TGCCAAGAGA ACATCTCTGA GGTTTATGCT GCATTGCTGG GCTGCATGAG TGATTACACC 
ACAGACAGCA GAGGCGATGT GGGAGCCTGG GTTCGTGAGG CTTCCATGAC CAGCCTCATG 
GATTTGATGC TTTTGCTGGC ACAGACTGAG CCAGTGCTGA TTGAGGCCCA CATCTGTGAG 
CGTGTCATGT GTTGCGTTGC CCAGCAGGCA AGTGAGAAGA TTGATCGGTT CCGTGCTCAT 
GCTGCCCGGG TATTCCTGAC TCTCTTGCAC TTCGACAGCC CTCCCATTCC CCATGTTCCC 
CACCGCAAGG AACTGGAAAG TCTGTTTCCC AGGTCTGATG TGGCCACTGT GAACTGGAAT 
GCACCTTCAC AGGCCTTCCC TCTTATCACC CAGCTCTTGG GACTGCCTAC CTATAGATAC 
CATGTCCTGC TGGGGCTGGC CGTGTCCGTG GGTGGCTTGA CAGAGTCTAC GGTCAGGCAT 
TCTACACAGA GCCTCTTCGA GTACATGAAG GGGATTCAGA AGGATGCTCA GGTCCTGGAG 
AGCTTCAGTG AGACCCTCCT AAAGGTCTTT GAGGACAACC TCTTGAATGA CAGAGTGTCT 
GTATCGCTGC TGAAAATGTT GGACCAGCTG TTGGCCAATG GGTGCTTTGA CATCTTTACT 
GCCGAGGAGA ACCACCCCTT TTGTGTGAAG TTGCTCACAC TTTGTAAGGA AGAAATCAAG 
AAGTCAAAAG ACATCCAGAA ACTTCGGTCA AGCATTGCAG TGCTCTGTGG GATGGTGCAG 
TTCAGTGGTG ATGTGAGAAA GAAGGTTCTT CTGCAGCTCT TCCTCCTCCT TGGCCACCCT 
TTTCCTGTGG TGCTGATGGC TTTTCCTCCT GTTGACCTGC TGTTCTCATT CCAGATCCGG 
AAGAGCACAG CAAGCCAGGT ATATGAGATG GTGCTTACCT ATAGTGACTT GGTAGATGCT 
GAGGTGCTTG ATGAGGTCAT GTCTGTGCTC AGTGACACAG CCTGGGACTC AGAGCTTCCC 
GTTGTAAGAG GACAGCGGAA TCACCTGTGT GACCTCCTTG GTGTGCCCAG ACCCCAACTG 
GTTCCAAAGC CTGTTCCAGG AAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008766723.1
CDS	831..3797
Translation	MASFLDWSLCTLAHSSFQTIDGVITMDGMLQALAQIFKHGKREDCLPYANTVLQCLDGCRLPESSHTLLRKLGVKLVQRLGLTFLKPKVATWRYQRGCRSLAANLQLRAPGKSEQKLLSDSLTSDGDEDYDVPEGVETVIEQLLVGLKDKDTIVRWSAAKGIGRMAGRLPRELADDVVGSVLDCFSFQETDKAWHGGCLALAELGRRGLLLPSRLSEVVTVILKALTYDEKRGACSVGANVRDAACYVCWAFARAYEPQELTPFVTAISSALVIAAVFDRNVNCRRAASAAFQENVGRQGTFPHGIDILTTADYFAVGNISNCFLIISVFIAGFQEYTKPMIDHLVSMKINHWDGAIRELSAKALHNLTSLVPEYMATHVFPALLLMTQSPDLHTRHGAILACAEVTYALYELAAQSNRLVTDYLDEKAVQSLKQIHQQLCDRHLYRGLGGELMRQAVCILIEKLSLSRMPFKGDTVIEGWQWLINDTLRSLHLVSSHSREQIKEVAVSALAAVCNEYYKKEPGEAGSTITEELIPQYLAELRSPEEMTRCGFSSALGALPCFLLRGHLQQVLSGLRRVTCTTSPMDVSFAEARRDGLKAISRICQTVGVNTQGPPDEVICQENISEVYAALLGCMSDYTTDSRGDVGAWVREASMTSLMDLMLLLAQTEPVLIEAHICERVMCCVAQQASEKIDRFRAHAARVFLTLLHFDSPPIPHVPHRKELESLFPRSDVATVNWNAPSQAFPLITQLLGLPTYRYHVLLGLAVSVGGLTESTVRHSTQSLFEYMKGIQKDAQVLESFSETLLKVFEDNLLNDRVSVSLLKMLDQLLANGCFDIFTAEENHPFCVKLLTLCKEEIKKSKDIQKLRSSIAVLCGMVQFSGDVRKKVLLQLFLLLGHPFPVVLMAFPPVDLLFSFQIRKSTASQVYEMVLTYSDLVDAEVLDEVMSVLSDTAWDSELPVVRGQRNHLCDLLGVPRPQLVPKPVPGS

Target ORF information:

RefSeq Version	XM_008768501.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus tubulin folding cofactor D (Tbcd), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008768501.2

ORF Insert Sequence:

ATGGCCAGCT TTCTGGACTG GAGCCTTTGT ACCTTGGCCC ACTCTTCCTT CCAGACCATT 
GACGGTGTCA TTACCATGGA TGGGATGTTG CAGGCCCTGG CTCAAATATT TAAGCATGGA 
AAACGTGAAG ACTGTCTACC CTATGCCAAC ACTGTGCTCC AGTGCCTTGA TGGATGTAGA 
CTCCCTGAGA GCAGTCACAC CTTACTGCGG AAGCTTGGTG TGAAGCTTGT CCAGAGGCTG 
GGGCTGACTT TCCTGAAGCC AAAGGTGGCC ACGTGGAGGT ATCAGCGTGG CTGTCGGTCT 
TTGGCTGCCA ACCTGCAGCT CCGTGCTCCG GGTAAGAGTG AACAGAAGCT GCTGTCTGAT 
AGTTTGACCT CTGATGGTGA TGAAGACTAT GATGTCCCAG AGGGGGTGGA AACTGTGATA 
GAGCAACTGC TGGTTGGGCT GAAGGACAAG GACACGATAG TGCGGTGGTC TGCAGCTAAA 
GGAATTGGTA GGATGGCTGG AAGGCTTCCC AGAGAGCTGG CAGATGATGT GGTGGGGTCC 
GTGTTGGACT GTTTCAGCTT TCAGGAGACA GATAAGGCAT GGCATGGTGG ATGTCTGGCA 
CTGGCAGAGC TGGGTAGACG AGGCCTGTTG CTGCCATCGA GACTCTCAGA GGTTGTCACA 
GTGATCCTGA AGGCCCTTAC CTACGATGAG AAGCGGGGTG CCTGCAGCGT GGGGGCCAAC 
GTCAGGGATG CTGCCTGCTA TGTGTGCTGG GCTTTTGCAC GTGCCTACGA GCCTCAGGAG 
TTGACACCTT TCGTGACTGC CATTTCAAGT GCCCTGGTCA TTGCTGCTGT ATTTGACCGA 
AACGTGAACT GCAGAAGAGC AGCCTCTGCT GCCTTCCAGG AGAACGTGGG GAGACAGGGT 
ACTTTTCCTC ATGGAATTGA TATTTTGACT ACAGCAGACT ATTTTGCAGT TGGTAACATT 
TCTAACTGTT TCCTGATTAT AAGCGTGTTT ATTGCTGGCT TCCAGGAATA CACCAAGCCA 
ATGATCGACC ACCTGGTCTC CATGAAAATC AACCACTGGG ATGGGGCCAT CCGAGAACTG 
TCTGCAAAGG CACTGCACAA CCTGACTTCA CTAGTACCCG AGTACATGGC CACTCACGTA 
TTCCCAGCAC TACTGCTGAT GACACAGAGT CCAGATCTAC ACACGAGACA TGGTGCCATC 
CTTGCCTGTG CAGAGGTCAC CTATGCCCTG TACGAGCTGG CCGCACAGAG TAACAGGCTT 
GTCACAGACT ATCTGGATGA GAAGGCAGTG CAAAGCCTGA AGCAGATCCA CCAGCAGCTT 
TGTGATCGCC ATCTCTACCG GGGTCTGGGA GGAGAGCTTA TGAGACAAGC AGTGTGCATC 
TTAATAGAGA AATTGTCACT TTCCAGAATG CCCTTTAAAG GTGACACCGT TATTGAGGGA 
TGGCAGTGGC TGATAAATGA CACTCTCAGG AGCCTTCACC TTGTTTCAAG CCATTCCAGA 
GAACAGATCA AGGAGGTGGC TGTCTCTGCC CTGGCTGCCG TCTGCAATGA ATACTACAAG 
AAGGAGCCTG GGGAGGCAGG CTCTACCATC ACAGAGGAGC TGATTCCACA GTACCTTGCT 
GAACTCCGAA GCCCAGAGGA GATGACCCGT TGTGGCTTTT CCTCAGCCTT GGGTGCCCTC 
CCGTGTTTCC TTCTCAGGGG CCATCTGCAG CAGGTTCTCT CAGGTCTGAG AAGAGTCACC 
TGTACTACTT CTCCCATGGA TGTGAGCTTT GCTGAGGCCA GGAGAGACGG TTTGAAAGCA 
ATCTCGAGAA TTTGCCAGAC AGTTGGTGTG AACACACAGG GACCTCCAGA TGAAGTCATA 
TGCCAAGAGA ACATCTCTGA GGTTTATGCT GCATTGCTGG GCTGCATGAG TGATTACACC 
ACAGACAGCA GAGGCGATGT GGGAGCCTGG GTTCGTGAGG CTTCCATGAC CAGCCTCATG 
GATTTGATGC TTTTGCTGGC ACAGACTGAG CCAGTGCTGA TTGAGGCCCA CATCTGTGAG 
CGTGTCATGT GTTGCGTTGC CCAGCAGGCA AGTGAGAAGA TTGATCGGTT CCGTGCTCAT 
GCTGCCCGGG TATTCCTGAC TCTCTTGCAC TTCGACAGCC CTCCCATTCC CCATGTTCCC 
CACCGCAAGG AACTGGAAAG TCTGTTTCCC AGGTCTGATG TGGCCACTGT GAACTGGAAT 
GCACCTTCAC AGGCCTTCCC TCTTATCACC CAGCTCTTGG GACTGCCTAC CTATAGATAC 
CATGTCCTGC TGGGGCTGGC CGTGTCCGTG GGTGGCTTGA CAGAGTCTAC GGTCAGGCAT 
TCTACACAGA GCCTCTTCGA GTACATGAAG GGGATTCAGA AGGATGCTCA GGTCCTGGAG 
AGCTTCAGTG AGACCCTCCT AAAGGTCTTT GAGGACAACC TCTTGAATGA CAGAGTGTCT 
GTATCGCTGC TGAAAATGTT GGACCAGCTG TTGGCCAATG GGTGCTTTGA CATCTTTACT 
GCCGAGGAGA ACCACCCCTT TTGTGTGAAG TTGCTCACAC TTTGTAAGGA AGAAATCAAG 
AAGTCAAAAG ACATCCAGAA ACTTCGGTCA AGCATTGCAG TGCTCTGTGG GATGGTGCAG 
TTCAGTGGTG ATGTGAGAAA GAAGGTTCTT CTGCAGCTCT TCCTCCTCCT TGGCCACCCT 
TTTCCTGTGG TGCTGATGGC TTTTCCTCCT GTTGACCTGC TGTTCTCATT CCAGATCCGG 
AAGAGCACAG CAAGCCAGGT ATATGAGATG GTGCTTACCT ATAGTGACTT GGTAGATGCT 
GAGGTGCTTG ATGAGGTCAT GTCTGTGCTC AGTGACACAG CCTGGGACTC AGAGCTTCCC 
GTTGTAAGAG GACAGCGGAA TCACCTGTGT GACCTCCTTG GTGTGCCCAG ACCCCAACTG 
GTTCCAAAGC CTGTTCCAGG AAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tbcd ( XM_008768502.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008766724.1 Rattus norvegicus tubulin folding cofactor D (Tbcd), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ORa50817
Clone ID Related Accession (Same CDS sequence)	XM_008768501.2 , XM_008768502.2
Accession Version	XM_008768502.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2967bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	tubulin-specific chaperone D isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008768502.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGCT TTCTGGACTG GAGCCTTTGT ACCTTGGCCC ACTCTTCCTT CCAGACCATT 
GACGGTGTCA TTACCATGGA TGGGATGTTG CAGGCCCTGG CTCAAATATT TAAGCATGGA 
AAACGTGAAG ACTGTCTACC CTATGCCAAC ACTGTGCTCC AGTGCCTTGA TGGATGTAGA 
CTCCCTGAGA GCAGTCACAC CTTACTGCGG AAGCTTGGTG TGAAGCTTGT CCAGAGGCTG 
GGGCTGACTT TCCTGAAGCC AAAGGTGGCC ACGTGGAGGT ATCAGCGTGG CTGTCGGTCT 
TTGGCTGCCA ACCTGCAGCT CCGTGCTCCG GGTAAGAGTG AACAGAAGCT GCTGTCTGAT 
AGTTTGACCT CTGATGGTGA TGAAGACTAT GATGTCCCAG AGGGGGTGGA AACTGTGATA 
GAGCAACTGC TGGTTGGGCT GAAGGACAAG GACACGATAG TGCGGTGGTC TGCAGCTAAA 
GGAATTGGTA GGATGGCTGG AAGGCTTCCC AGAGAGCTGG CAGATGATGT GGTGGGGTCC 
GTGTTGGACT GTTTCAGCTT TCAGGAGACA GATAAGGCAT GGCATGGTGG ATGTCTGGCA 
CTGGCAGAGC TGGGTAGACG AGGCCTGTTG CTGCCATCGA GACTCTCAGA GGTTGTCACA 
GTGATCCTGA AGGCCCTTAC CTACGATGAG AAGCGGGGTG CCTGCAGCGT GGGGGCCAAC 
GTCAGGGATG CTGCCTGCTA TGTGTGCTGG GCTTTTGCAC GTGCCTACGA GCCTCAGGAG 
TTGACACCTT TCGTGACTGC CATTTCAAGT GCCCTGGTCA TTGCTGCTGT ATTTGACCGA 
AACGTGAACT GCAGAAGAGC AGCCTCTGCT GCCTTCCAGG AGAACGTGGG GAGACAGGGT 
ACTTTTCCTC ATGGAATTGA TATTTTGACT ACAGCAGACT ATTTTGCAGT TGGTAACATT 
TCTAACTGTT TCCTGATTAT AAGCGTGTTT ATTGCTGGCT TCCAGGAATA CACCAAGCCA 
ATGATCGACC ACCTGGTCTC CATGAAAATC AACCACTGGG ATGGGGCCAT CCGAGAACTG 
TCTGCAAAGG CACTGCACAA CCTGACTTCA CTAGTACCCG AGTACATGGC CACTCACGTA 
TTCCCAGCAC TACTGCTGAT GACACAGAGT CCAGATCTAC ACACGAGACA TGGTGCCATC 
CTTGCCTGTG CAGAGGTCAC CTATGCCCTG TACGAGCTGG CCGCACAGAG TAACAGGCTT 
GTCACAGACT ATCTGGATGA GAAGGCAGTG CAAAGCCTGA AGCAGATCCA CCAGCAGCTT 
TGTGATCGCC ATCTCTACCG GGGTCTGGGA GGAGAGCTTA TGAGACAAGC AGTGTGCATC 
TTAATAGAGA AATTGTCACT TTCCAGAATG CCCTTTAAAG GTGACACCGT TATTGAGGGA 
TGGCAGTGGC TGATAAATGA CACTCTCAGG AGCCTTCACC TTGTTTCAAG CCATTCCAGA 
GAACAGATCA AGGAGGTGGC TGTCTCTGCC CTGGCTGCCG TCTGCAATGA ATACTACAAG 
AAGGAGCCTG GGGAGGCAGG CTCTACCATC ACAGAGGAGC TGATTCCACA GTACCTTGCT 
GAACTCCGAA GCCCAGAGGA GATGACCCGT TGTGGCTTTT CCTCAGCCTT GGGTGCCCTC 
CCGTGTTTCC TTCTCAGGGG CCATCTGCAG CAGGTTCTCT CAGGTCTGAG AAGAGTCACC 
TGTACTACTT CTCCCATGGA TGTGAGCTTT GCTGAGGCCA GGAGAGACGG TTTGAAAGCA 
ATCTCGAGAA TTTGCCAGAC AGTTGGTGTG AACACACAGG GACCTCCAGA TGAAGTCATA 
TGCCAAGAGA ACATCTCTGA GGTTTATGCT GCATTGCTGG GCTGCATGAG TGATTACACC 
ACAGACAGCA GAGGCGATGT GGGAGCCTGG GTTCGTGAGG CTTCCATGAC CAGCCTCATG 
GATTTGATGC TTTTGCTGGC ACAGACTGAG CCAGTGCTGA TTGAGGCCCA CATCTGTGAG 
CGTGTCATGT GTTGCGTTGC CCAGCAGGCA AGTGAGAAGA TTGATCGGTT CCGTGCTCAT 
GCTGCCCGGG TATTCCTGAC TCTCTTGCAC TTCGACAGCC CTCCCATTCC CCATGTTCCC 
CACCGCAAGG AACTGGAAAG TCTGTTTCCC AGGTCTGATG TGGCCACTGT GAACTGGAAT 
GCACCTTCAC AGGCCTTCCC TCTTATCACC CAGCTCTTGG GACTGCCTAC CTATAGATAC 
CATGTCCTGC TGGGGCTGGC CGTGTCCGTG GGTGGCTTGA CAGAGTCTAC GGTCAGGCAT 
TCTACACAGA GCCTCTTCGA GTACATGAAG GGGATTCAGA AGGATGCTCA GGTCCTGGAG 
AGCTTCAGTG AGACCCTCCT AAAGGTCTTT GAGGACAACC TCTTGAATGA CAGAGTGTCT 
GTATCGCTGC TGAAAATGTT GGACCAGCTG TTGGCCAATG GGTGCTTTGA CATCTTTACT 
GCCGAGGAGA ACCACCCCTT TTGTGTGAAG TTGCTCACAC TTTGTAAGGA AGAAATCAAG 
AAGTCAAAAG ACATCCAGAA ACTTCGGTCA AGCATTGCAG TGCTCTGTGG GATGGTGCAG 
TTCAGTGGTG ATGTGAGAAA GAAGGTTCTT CTGCAGCTCT TCCTCCTCCT TGGCCACCCT 
TTTCCTGTGG TGCTGATGGC TTTTCCTCCT GTTGACCTGC TGTTCTCATT CCAGATCCGG 
AAGAGCACAG CAAGCCAGGT ATATGAGATG GTGCTTACCT ATAGTGACTT GGTAGATGCT 
GAGGTGCTTG ATGAGGTCAT GTCTGTGCTC AGTGACACAG CCTGGGACTC AGAGCTTCCC 
GTTGTAAGAG GACAGCGGAA TCACCTGTGT GACCTCCTTG GTGTGCCCAG ACCCCAACTG 
GTTCCAAAGC CTGTTCCAGG AAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008766724.1
CDS	183..3149
Translation	MASFLDWSLCTLAHSSFQTIDGVITMDGMLQALAQIFKHGKREDCLPYANTVLQCLDGCRLPESSHTLLRKLGVKLVQRLGLTFLKPKVATWRYQRGCRSLAANLQLRAPGKSEQKLLSDSLTSDGDEDYDVPEGVETVIEQLLVGLKDKDTIVRWSAAKGIGRMAGRLPRELADDVVGSVLDCFSFQETDKAWHGGCLALAELGRRGLLLPSRLSEVVTVILKALTYDEKRGACSVGANVRDAACYVCWAFARAYEPQELTPFVTAISSALVIAAVFDRNVNCRRAASAAFQENVGRQGTFPHGIDILTTADYFAVGNISNCFLIISVFIAGFQEYTKPMIDHLVSMKINHWDGAIRELSAKALHNLTSLVPEYMATHVFPALLLMTQSPDLHTRHGAILACAEVTYALYELAAQSNRLVTDYLDEKAVQSLKQIHQQLCDRHLYRGLGGELMRQAVCILIEKLSLSRMPFKGDTVIEGWQWLINDTLRSLHLVSSHSREQIKEVAVSALAAVCNEYYKKEPGEAGSTITEELIPQYLAELRSPEEMTRCGFSSALGALPCFLLRGHLQQVLSGLRRVTCTTSPMDVSFAEARRDGLKAISRICQTVGVNTQGPPDEVICQENISEVYAALLGCMSDYTTDSRGDVGAWVREASMTSLMDLMLLLAQTEPVLIEAHICERVMCCVAQQASEKIDRFRAHAARVFLTLLHFDSPPIPHVPHRKELESLFPRSDVATVNWNAPSQAFPLITQLLGLPTYRYHVLLGLAVSVGGLTESTVRHSTQSLFEYMKGIQKDAQVLESFSETLLKVFEDNLLNDRVSVSLLKMLDQLLANGCFDIFTAEENHPFCVKLLTLCKEEIKKSKDIQKLRSSIAVLCGMVQFSGDVRKKVLLQLFLLLGHPFPVVLMAFPPVDLLFSFQIRKSTASQVYEMVLTYSDLVDAEVLDEVMSVLSDTAWDSELPVVRGQRNHLCDLLGVPRPQLVPKPVPGS

Target ORF information:

RefSeq Version	XM_008768502.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus tubulin folding cofactor D (Tbcd), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008768502.2

ORF Insert Sequence:

ATGGCCAGCT TTCTGGACTG GAGCCTTTGT ACCTTGGCCC ACTCTTCCTT CCAGACCATT 
GACGGTGTCA TTACCATGGA TGGGATGTTG CAGGCCCTGG CTCAAATATT TAAGCATGGA 
AAACGTGAAG ACTGTCTACC CTATGCCAAC ACTGTGCTCC AGTGCCTTGA TGGATGTAGA 
CTCCCTGAGA GCAGTCACAC CTTACTGCGG AAGCTTGGTG TGAAGCTTGT CCAGAGGCTG 
GGGCTGACTT TCCTGAAGCC AAAGGTGGCC ACGTGGAGGT ATCAGCGTGG CTGTCGGTCT 
TTGGCTGCCA ACCTGCAGCT CCGTGCTCCG GGTAAGAGTG AACAGAAGCT GCTGTCTGAT 
AGTTTGACCT CTGATGGTGA TGAAGACTAT GATGTCCCAG AGGGGGTGGA AACTGTGATA 
GAGCAACTGC TGGTTGGGCT GAAGGACAAG GACACGATAG TGCGGTGGTC TGCAGCTAAA 
GGAATTGGTA GGATGGCTGG AAGGCTTCCC AGAGAGCTGG CAGATGATGT GGTGGGGTCC 
GTGTTGGACT GTTTCAGCTT TCAGGAGACA GATAAGGCAT GGCATGGTGG ATGTCTGGCA 
CTGGCAGAGC TGGGTAGACG AGGCCTGTTG CTGCCATCGA GACTCTCAGA GGTTGTCACA 
GTGATCCTGA AGGCCCTTAC CTACGATGAG AAGCGGGGTG CCTGCAGCGT GGGGGCCAAC 
GTCAGGGATG CTGCCTGCTA TGTGTGCTGG GCTTTTGCAC GTGCCTACGA GCCTCAGGAG 
TTGACACCTT TCGTGACTGC CATTTCAAGT GCCCTGGTCA TTGCTGCTGT ATTTGACCGA 
AACGTGAACT GCAGAAGAGC AGCCTCTGCT GCCTTCCAGG AGAACGTGGG GAGACAGGGT 
ACTTTTCCTC ATGGAATTGA TATTTTGACT ACAGCAGACT ATTTTGCAGT TGGTAACATT 
TCTAACTGTT TCCTGATTAT AAGCGTGTTT ATTGCTGGCT TCCAGGAATA CACCAAGCCA 
ATGATCGACC ACCTGGTCTC CATGAAAATC AACCACTGGG ATGGGGCCAT CCGAGAACTG 
TCTGCAAAGG CACTGCACAA CCTGACTTCA CTAGTACCCG AGTACATGGC CACTCACGTA 
TTCCCAGCAC TACTGCTGAT GACACAGAGT CCAGATCTAC ACACGAGACA TGGTGCCATC 
CTTGCCTGTG CAGAGGTCAC CTATGCCCTG TACGAGCTGG CCGCACAGAG TAACAGGCTT 
GTCACAGACT ATCTGGATGA GAAGGCAGTG CAAAGCCTGA AGCAGATCCA CCAGCAGCTT 
TGTGATCGCC ATCTCTACCG GGGTCTGGGA GGAGAGCTTA TGAGACAAGC AGTGTGCATC 
TTAATAGAGA AATTGTCACT TTCCAGAATG CCCTTTAAAG GTGACACCGT TATTGAGGGA 
TGGCAGTGGC TGATAAATGA CACTCTCAGG AGCCTTCACC TTGTTTCAAG CCATTCCAGA 
GAACAGATCA AGGAGGTGGC TGTCTCTGCC CTGGCTGCCG TCTGCAATGA ATACTACAAG 
AAGGAGCCTG GGGAGGCAGG CTCTACCATC ACAGAGGAGC TGATTCCACA GTACCTTGCT 
GAACTCCGAA GCCCAGAGGA GATGACCCGT TGTGGCTTTT CCTCAGCCTT GGGTGCCCTC 
CCGTGTTTCC TTCTCAGGGG CCATCTGCAG CAGGTTCTCT CAGGTCTGAG AAGAGTCACC 
TGTACTACTT CTCCCATGGA TGTGAGCTTT GCTGAGGCCA GGAGAGACGG TTTGAAAGCA 
ATCTCGAGAA TTTGCCAGAC AGTTGGTGTG AACACACAGG GACCTCCAGA TGAAGTCATA 
TGCCAAGAGA ACATCTCTGA GGTTTATGCT GCATTGCTGG GCTGCATGAG TGATTACACC 
ACAGACAGCA GAGGCGATGT GGGAGCCTGG GTTCGTGAGG CTTCCATGAC CAGCCTCATG 
GATTTGATGC TTTTGCTGGC ACAGACTGAG CCAGTGCTGA TTGAGGCCCA CATCTGTGAG 
CGTGTCATGT GTTGCGTTGC CCAGCAGGCA AGTGAGAAGA TTGATCGGTT CCGTGCTCAT 
GCTGCCCGGG TATTCCTGAC TCTCTTGCAC TTCGACAGCC CTCCCATTCC CCATGTTCCC 
CACCGCAAGG AACTGGAAAG TCTGTTTCCC AGGTCTGATG TGGCCACTGT GAACTGGAAT 
GCACCTTCAC AGGCCTTCCC TCTTATCACC CAGCTCTTGG GACTGCCTAC CTATAGATAC 
CATGTCCTGC TGGGGCTGGC CGTGTCCGTG GGTGGCTTGA CAGAGTCTAC GGTCAGGCAT 
TCTACACAGA GCCTCTTCGA GTACATGAAG GGGATTCAGA AGGATGCTCA GGTCCTGGAG 
AGCTTCAGTG AGACCCTCCT AAAGGTCTTT GAGGACAACC TCTTGAATGA CAGAGTGTCT 
GTATCGCTGC TGAAAATGTT GGACCAGCTG TTGGCCAATG GGTGCTTTGA CATCTTTACT 
GCCGAGGAGA ACCACCCCTT TTGTGTGAAG TTGCTCACAC TTTGTAAGGA AGAAATCAAG 
AAGTCAAAAG ACATCCAGAA ACTTCGGTCA AGCATTGCAG TGCTCTGTGG GATGGTGCAG 
TTCAGTGGTG ATGTGAGAAA GAAGGTTCTT CTGCAGCTCT TCCTCCTCCT TGGCCACCCT 
TTTCCTGTGG TGCTGATGGC TTTTCCTCCT GTTGACCTGC TGTTCTCATT CCAGATCCGG 
AAGAGCACAG CAAGCCAGGT ATATGAGATG GTGCTTACCT ATAGTGACTT GGTAGATGCT 
GAGGTGCTTG ATGAGGTCAT GTCTGTGCTC AGTGACACAG CCTGGGACTC AGAGCTTCCC 
GTTGTAAGAG GACAGCGGAA TCACCTGTGT GACCTCCTTG GTGTGCCCAG ACCCCAACTG 
GTTCCAAAGC CTGTTCCAGG AAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tbcd ( NM_001271364.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001258293.1 Rattus norvegicus tubulin folding cofactor D (Tbcd), mRNA.

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CloneID	ORa11083
Clone ID Related Accession (Same CDS sequence)	NM_001271364.1
Accession Version	NM_001271364.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3594bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-30
Organism	Rattus norvegicus(Norway rat)
Product	tubulin-specific chaperone D
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AABR07030942.1 and AABR07030943.1. On Sep 18, 2012 this sequence version replaced XM_002730075.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGTACTGA GCAACGAGCC GGCTGCGAGC GCCGCGGAGG AGGAGGTGGA GGATGATGCG 
CTTGTCCGCG CCTCCACCCT GGAAGCGTTC GGCGAGAGTG CGGAGACCCG CGCGCTGCTC 
CGCAGCTTGC CGGCGGTGCA CCGCGAGCGC GCGGCCCGGG AGGTGGCGGA GGAGCGGTTC 
CGCGTAATTA TGGACAAATA CCAGGAGCAG CCCCACCTCC TGGACCCACA CCTCGAGTGG 
ATGATGAACT CACTGTTGGA CATAGTACAG GATGAGACAT CTCTGCCCGA CCTTGTACAT 
CTGGCTTTTA AATTTCTGTA TATCATCACC AAGGTTCGTG GATATAAAGT ATTTCTGCGT 
CTGTTTCCCC ATGAAGTAGC TGATGTTCAG CCTGTGTTAG ATATGTTCAC AGGACAGAAC 
CCTAAAGACC ATGAGACATG GGAAACTCGC TACATGCTTT TACTGTGGCT GTCTGTGACC 
TGCTTGATCC CTTTTGATTT TTCACGCCTT GATGGGAACC TGCCCACTCA ATCTGGAGAA 
ACACGGATGC CCATAATGGA CCGAATTCTG CAAATAGCAC AGTCTTACTT GGTGGTCAGT 
GACAAGGCGC GAGATGCGGC TGCTGTCCTT GTGTCCAAGT TTATCACACG TCCTGATGTC 
AAGCAGAGAA AGATGGCCAG CTTTCTGGAC TGGAGCCTTT GTACCTTGGC CCACTCTTCC 
TTCCAGACCA TTGACGGTGT CATTACCATG GATGGGATGT TGCAGGCCCT GGCTCAAATA 
TTTAAGCATG GAAAACGTGA AGACTGTCTA CCCTATGCCA ACACTGTGCT CCAGTGCCTT 
GATGGATGTA GACTCCCTGA GAGCAGTCAC ACCTTACTGC GGAAGCTTGG TGTGAAGCTT 
GTCCAGAGGC TGGGGCTGAC TTTCCTGAAG CCAAAGGTGG CCACGTGGAG GTATCAGCGT 
GGCTGTCGGT CTTTGGCTGC CAACCTGCAG CTCCGTGCTC CGGGTAAGAG TGAACAGAAG 
CTGCTGTCTG ATAGTTTGAC CTCTGATGGT GATGAAGACT ATGATGTCCC AGAGGGGGTG 
GAAACTGTGA TAGAGCAACT GCTGGTTGGG CTGAAGGACA AGGACACGAT AGTGCGGTGG 
TCTGCAGCTA AAGGAATTGG TAGGATGGCT GGAAGGCTTC CCAGAGAGCT GGCAGATGAT 
GTGGTGGGGT CCGTGTTGGA CTGTTTCAGC TTTCAGGAGA CAGATAAGGC ATGGCATGGT 
GGATGTCTGG CACTGGCAGA GCTGGGTAGA CGAGGCCTGT TGCTGCCATC GAGACTCTCA 
GAGGTTGTCA CAGTGATCCT GAAGGCCCTT ACCTACGATG AGAAGCGGGG TGCCTGCAGC 
GTGGGGGCCA ACGTCAGGGA TGCTGCCTGC TATGTGTGCT GGGCTTTTGC ACGTGCCTAC 
GAGCCTCAGG AGTTGACACC TTTCGTGACT GCCATTTCAA GTGCCCTGGT CATTGCTGCT 
GTATTTGACC GAAACGTGAA CTGCAGAAGA GCAGCCTCTG CTGCCTTCCA GGAGAACGTG 
GGGAGACAGG GTACTTTTCC TCATGGAATT GATATTTTGA CTACAGCAGA CTATTTTGCA 
GTTGGTAACA TTTCTAACTG TTTCCTGATT ATAAGCGTGT TTATTGCTGG CTTCCAGGAA 
TACACCAAGC CAATGATCGA CCACCTGGTC TCCATGAAAA TCAACCACTG GGATGGGGCC 
ATCCGAGAAC TGTCTGCAAA GGCACTGCAC AACCTGACTT CACTAGTACC CGAGTACATG 
GCCACTCACG TATTCCCAGC ACTACTGCTG ATGACACAGA GTCCAGATCT ACACACGAGA 
CATGGTGCCA TCCTTGCCTG TGCAGAGGTC ACCTATGCCC TGTACGAGCT GGCCGCACAG 
AGTAACAGGC TTGTCACAGA CTATCTGGAT GAGAAGGCAG TGCAAAGCCT GAAGCAGATC 
CACCAGCAGC TTTGTGATCG CCATCTCTAC CGGGGTCTGG GAGGAGAGCT TATGAGACAA 
GCAGTGTGCA TCTTAATAGA GAAATTGTCA CTTTCCAGAA TGCCCTTTAA AGGTGACACC 
GTTATTGAGG GATGGCAGTG GCTGATAAAT GACACTCTCA GGAGCCTTCA CCTTGTTTCA 
AGCCATTCCA GAGAACAGAT CAAGGAGGTG GCTGTCTCTG CCCTGGCTGC CGTCTGCAAT 
GAATACTACA AGAAGGAGCC TGGGGAGGCA GGCTCTACCA TCACAGAGGA GCTGATTCCA 
CAGTACCTTG CTGAACTCCG AAGCCCAGAG GAGATGACCC GTTGTGGCTT TTCCTCAGCC 
TTGGGTGCCC TCCCGTGTTT CCTTCTCAGG GGCCATCTGC AGCAGGTTCT CTCAGGTCTG 
AGAAGAGTCA CCTGTACTAC TTCTCCCATG GATGTGAGCT TTGCTGAGGC CAGGAGAGAC 
GGTTTGAAAG CAATCTCGAG AATTTGCCAG ACAGTTGGTG TGAACACACA GGGACCTCCA 
GATGAAGTCA TATGCCAAGA GAACATCTCT GAGGTTTATG CTGCATTGCT GGGCTGCATG 
AGTGATTACA CCACAGACAG CAGAGGCGAT GTGGGAGCCT GGGTTCGTGA GGCTTCCATG 
ACCAGCCTCA TGGATTTGAT GCTTTTGCTG GCACAGACTG AGCCAGTGCT GATTGAGGCC 
CACATCTGTG AGCGTGTCAT GTGTTGCGTT GCCCAGCAGG CAAGTGAGAA GATTGATCGG 
TTCCGTGCTC ATGCTGCCCG GGTATTCCTG ACTCTCTTGC ACTTCGACAG CCCTCCCATT 
CCCCATGTTC CCCACCGCAA GGAACTGGAA AGTCTGTTTC CCAGGTCTGA TGTGGCCACT 
GTGAACTGGA ATGCACCTTC ACAGGCCTTC CCTCTTATCA CCCAGCTCTT GGGACTGCCT 
ACCTATAGAT ACCATGTCCT GCTGGGGCTG GCCGTGTCCG TGGGTGGCTT GACAGAGTCT 
ACGGTCAGGC ATTCTACACA GAGCCTCTTC GAGTACATGA AGGGGATTCA GAAGGATGCT 
CAGGTCCTGG AGAGCTTCAG TGAGACCCTC CTAAAGGTCT TTGAGGACAA CCTCTTGAAT 
GACAGAGTGT CTGTATCGCT GCTGAAAATG TTGGACCAGC TGTTGGCCAA TGGGTGCTTT 
GACATCTTTA CTGCCGAGGA GAACCACCCC TTTTGTGTGA AGTTGCTCAC ACTTTGTAAG 
GAAGAAATCA AGAAGTCAAA AGACATCCAG AAACTTCGGT CAAGCATTGC AGTGCTCTGT 
GGGATGGTGC AGTTCAGTGG TGATGTGAGA AAGAAGGTTC TTCTGCAGCT CTTCCTCCTC 
CTTGGCCACC CTTTTCCTGT GATCCGGAAG AGCACAGCAA GCCAGGTATA TGAGATGGTG 
CTTACCTATA GTGACTTGGT AGATGCTGAG GTGCTTGATG AGGTCATGTC TGTGCTCAGT 
GACACAGCCT GGGACTCAGA GCTTCCCGTT GTAAGAGGAC AGCGGAATCA CCTGTGTGAC 
CTCCTTGGTG TGCCCAGACC CCAACTGGTT CCAAAGCCTG TTCCAGGAAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001258293.1
CDS	45..3638
Translation	MVLSNEPAASAAEEEVEDDALVRASTLEAFGESAETRALLRSLPAVHRERAAREVAEERFRVIMDKYQEQPHLLDPHLEWMMNSLLDIVQDETSLPDLVHLAFKFLYIITKVRGYKVFLRLFPHEVADVQPVLDMFTGQNPKDHETWETRYMLLLWLSVTCLIPFDFSRLDGNLPTQSGETRMPIMDRILQIAQSYLVVSDKARDAAAVLVSKFITRPDVKQRKMASFLDWSLCTLAHSSFQTIDGVITMDGMLQALAQIFKHGKREDCLPYANTVLQCLDGCRLPESSHTLLRKLGVKLVQRLGLTFLKPKVATWRYQRGCRSLAANLQLRAPGKSEQKLLSDSLTSDGDEDYDVPEGVETVIEQLLVGLKDKDTIVRWSAAKGIGRMAGRLPRELADDVVGSVLDCFSFQETDKAWHGGCLALAELGRRGLLLPSRLSEVVTVILKALTYDEKRGACSVGANVRDAACYVCWAFARAYEPQELTPFVTAISSALVIAAVFDRNVNCRRAASAAFQENVGRQGTFPHGIDILTTADYFAVGNISNCFLIISVFIAGFQEYTKPMIDHLVSMKINHWDGAIRELSAKALHNLTSLVPEYMATHVFPALLLMTQSPDLHTRHGAILACAEVTYALYELAAQSNRLVTDYLDEKAVQSLKQIHQQLCDRHLYRGLGGELMRQAVCILIEKLSLSRMPFKGDTVIEGWQWLINDTLRSLHLVSSHSREQIKEVAVSALAAVCNEYYKKEPGEAGSTITEELIPQYLAELRSPEEMTRCGFSSALGALPCFLLRGHLQQVLSGLRRVTCTTSPMDVSFAEARRDGLKAISRICQTVGVNTQGPPDEVICQENISEVYAALLGCMSDYTTDSRGDVGAWVREASMTSLMDLMLLLAQTEPVLIEAHICERVMCCVAQQASEKIDRFRAHAARVFLTLLHFDSPPIPHVPHRKELESLFPRSDVATVNWNAPSQAFPLITQLLGLPTYRYHVLLGLAVSVGGLTESTVRHSTQSLFEYMKGIQKDAQVLESFSETLLKVFEDNLLNDRVSVSLLKMLDQLLANGCFDIFTAEENHPFCVKLLTLCKEEIKKSKDIQKLRSSIAVLCGMVQFSGDVRKKVLLQLFLLLGHPFPVIRKSTASQVYEMVLTYSDLVDAEVLDEVMSVLSDTAWDSELPVVRGQRNHLCDLLGVPRPQLVPKPVPGS

Target ORF information:

RefSeq Version	NM_001271364.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus tubulin folding cofactor D (Tbcd), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001271364.1

ORF Insert Sequence:

ATGGTACTGA GCAACGAGCC GGCTGCGAGC GCCGCGGAGG AGGAGGTGGA GGATGATGCG 
CTTGTCCGCG CCTCCACCCT GGAAGCGTTC GGCGAGAGTG CGGAGACCCG CGCGCTGCTC 
CGCAGCTTGC CGGCGGTGCA CCGCGAGCGC GCGGCCCGGG AGGTGGCGGA GGAGCGGTTC 
CGCGTAATTA TGGACAAATA CCAGGAGCAG CCCCACCTCC TGGACCCACA CCTCGAGTGG 
ATGATGAACT CACTGTTGGA CATAGTACAG GATGAGACAT CTCTGCCCGA CCTTGTACAT 
CTGGCTTTTA AATTTCTGTA TATCATCACC AAGGTTCGTG GATATAAAGT ATTTCTGCGT 
CTGTTTCCCC ATGAAGTAGC TGATGTTCAG CCTGTGTTAG ATATGTTCAC AGGACAGAAC 
CCTAAAGACC ATGAGACATG GGAAACTCGC TACATGCTTT TACTGTGGCT GTCTGTGACC 
TGCTTGATCC CTTTTGATTT TTCACGCCTT GATGGGAACC TGCCCACTCA ATCTGGAGAA 
ACACGGATGC CCATAATGGA CCGAATTCTG CAAATAGCAC AGTCTTACTT GGTGGTCAGT 
GACAAGGCGC GAGATGCGGC TGCTGTCCTT GTGTCCAAGT TTATCACACG TCCTGATGTC 
AAGCAGAGAA AGATGGCCAG CTTTCTGGAC TGGAGCCTTT GTACCTTGGC CCACTCTTCC 
TTCCAGACCA TTGACGGTGT CATTACCATG GATGGGATGT TGCAGGCCCT GGCTCAAATA 
TTTAAGCATG GAAAACGTGA AGACTGTCTA CCCTATGCCA ACACTGTGCT CCAGTGCCTT 
GATGGATGTA GACTCCCTGA GAGCAGTCAC ACCTTACTGC GGAAGCTTGG TGTGAAGCTT 
GTCCAGAGGC TGGGGCTGAC TTTCCTGAAG CCAAAGGTGG CCACGTGGAG GTATCAGCGT 
GGCTGTCGGT CTTTGGCTGC CAACCTGCAG CTCCGTGCTC CGGGTAAGAG TGAACAGAAG 
CTGCTGTCTG ATAGTTTGAC CTCTGATGGT GATGAAGACT ATGATGTCCC AGAGGGGGTG 
GAAACTGTGA TAGAGCAACT GCTGGTTGGG CTGAAGGACA AGGACACGAT AGTGCGGTGG 
TCTGCAGCTA AAGGAATTGG TAGGATGGCT GGAAGGCTTC CCAGAGAGCT GGCAGATGAT 
GTGGTGGGGT CCGTGTTGGA CTGTTTCAGC TTTCAGGAGA CAGATAAGGC ATGGCATGGT 
GGATGTCTGG CACTGGCAGA GCTGGGTAGA CGAGGCCTGT TGCTGCCATC GAGACTCTCA 
GAGGTTGTCA CAGTGATCCT GAAGGCCCTT ACCTACGATG AGAAGCGGGG TGCCTGCAGC 
GTGGGGGCCA ACGTCAGGGA TGCTGCCTGC TATGTGTGCT GGGCTTTTGC ACGTGCCTAC 
GAGCCTCAGG AGTTGACACC TTTCGTGACT GCCATTTCAA GTGCCCTGGT CATTGCTGCT 
GTATTTGACC GAAACGTGAA CTGCAGAAGA GCAGCCTCTG CTGCCTTCCA GGAGAACGTG 
GGGAGACAGG GTACTTTTCC TCATGGAATT GATATTTTGA CTACAGCAGA CTATTTTGCA 
GTTGGTAACA TTTCTAACTG TTTCCTGATT ATAAGCGTGT TTATTGCTGG CTTCCAGGAA 
TACACCAAGC CAATGATCGA CCACCTGGTC TCCATGAAAA TCAACCACTG GGATGGGGCC 
ATCCGAGAAC TGTCTGCAAA GGCACTGCAC AACCTGACTT CACTAGTACC CGAGTACATG 
GCCACTCACG TATTCCCAGC ACTACTGCTG ATGACACAGA GTCCAGATCT ACACACGAGA 
CATGGTGCCA TCCTTGCCTG TGCAGAGGTC ACCTATGCCC TGTACGAGCT GGCCGCACAG 
AGTAACAGGC TTGTCACAGA CTATCTGGAT GAGAAGGCAG TGCAAAGCCT GAAGCAGATC 
CACCAGCAGC TTTGTGATCG CCATCTCTAC CGGGGTCTGG GAGGAGAGCT TATGAGACAA 
GCAGTGTGCA TCTTAATAGA GAAATTGTCA CTTTCCAGAA TGCCCTTTAA AGGTGACACC 
GTTATTGAGG GATGGCAGTG GCTGATAAAT GACACTCTCA GGAGCCTTCA CCTTGTTTCA 
AGCCATTCCA GAGAACAGAT CAAGGAGGTG GCTGTCTCTG CCCTGGCTGC CGTCTGCAAT 
GAATACTACA AGAAGGAGCC TGGGGAGGCA GGCTCTACCA TCACAGAGGA GCTGATTCCA 
CAGTACCTTG CTGAACTCCG AAGCCCAGAG GAGATGACCC GTTGTGGCTT TTCCTCAGCC 
TTGGGTGCCC TCCCGTGTTT CCTTCTCAGG GGCCATCTGC AGCAGGTTCT CTCAGGTCTG 
AGAAGAGTCA CCTGTACTAC TTCTCCCATG GATGTGAGCT TTGCTGAGGC CAGGAGAGAC 
GGTTTGAAAG CAATCTCGAG AATTTGCCAG ACAGTTGGTG TGAACACACA GGGACCTCCA 
GATGAAGTCA TATGCCAAGA GAACATCTCT GAGGTTTATG CTGCATTGCT GGGCTGCATG 
AGTGATTACA CCACAGACAG CAGAGGCGAT GTGGGAGCCT GGGTTCGTGA GGCTTCCATG 
ACCAGCCTCA TGGATTTGAT GCTTTTGCTG GCACAGACTG AGCCAGTGCT GATTGAGGCC 
CACATCTGTG AGCGTGTCAT GTGTTGCGTT GCCCAGCAGG CAAGTGAGAA GATTGATCGG 
TTCCGTGCTC ATGCTGCCCG GGTATTCCTG ACTCTCTTGC ACTTCGACAG CCCTCCCATT 
CCCCATGTTC CCCACCGCAA GGAACTGGAA AGTCTGTTTC CCAGGTCTGA TGTGGCCACT 
GTGAACTGGA ATGCACCTTC ACAGGCCTTC CCTCTTATCA CCCAGCTCTT GGGACTGCCT 
ACCTATAGAT ACCATGTCCT GCTGGGGCTG GCCGTGTCCG TGGGTGGCTT GACAGAGTCT 
ACGGTCAGGC ATTCTACACA GAGCCTCTTC GAGTACATGA AGGGGATTCA GAAGGATGCT 
CAGGTCCTGG AGAGCTTCAG TGAGACCCTC CTAAAGGTCT TTGAGGACAA CCTCTTGAAT 
GACAGAGTGT CTGTATCGCT GCTGAAAATG TTGGACCAGC TGTTGGCCAA TGGGTGCTTT 
GACATCTTTA CTGCCGAGGA GAACCACCCC TTTTGTGTGA AGTTGCTCAC ACTTTGTAAG 
GAAGAAATCA AGAAGTCAAA AGACATCCAG AAACTTCGGT CAAGCATTGC AGTGCTCTGT 
GGGATGGTGC AGTTCAGTGG TGATGTGAGA AAGAAGGTTC TTCTGCAGCT CTTCCTCCTC 
CTTGGCCACC CTTTTCCTGT GATCCGGAAG AGCACAGCAA GCCAGGTATA TGAGATGGTG 
CTTACCTATA GTGACTTGGT AGATGCTGAG GTGCTTGATG AGGTCATGTC TGTGCTCAGT 
GACACAGCCT GGGACTCAGA GCTTCCCGTT GTAAGAGGAC AGCGGAATCA CCTGTGTGAC 
CTCCTTGGTG TGCCCAGACC CCAACTGGTT CCAAAGCCTG TTCCAGGAAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tbcd ( XM_006247861.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006247923.1 Rattus norvegicus tubulin folding cofactor D (Tbcd), transcript variant X1, mRNA.

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CloneID	ORa34010
Clone ID Related Accession (Same CDS sequence)	XM_006247861.3
Accession Version	XM_006247861.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3639bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	tubulin-specific chaperone D isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006247861.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTACTGA GCAACGAGCC GGCTGCGAGC GCCGCGGAGG AGGAGGTGGA GGATGATGCG 
CTTGTCCGCG CCTCCACCCT GGAAGCGTTC GGCGAGAGTG CGGAGACCCG CGCGCTGCTC 
CGCAGCTTGC CGGCGGTGCA CCGCGAGCGC GCGGCCCGGG AGGTGGCGGA GGAGCGGTTC 
CGCGTAATTA TGGACAAATA CCAGGAGCAG CCCCACCTCC TGGACCCACA CCTCGAGTGG 
ATGATGAACT CACTGTTGGA CATAGTACAG GATGAGACAT CTCTGCCCGA CCTTGTACAT 
CTGGCTTTTA AATTTCTGTA TATCATCACC AAGGTTCGTG GATATAAAGT ATTTCTGCGT 
CTGTTTCCCC ATGAAGTAGC TGATGTTCAG CCTGTGTTAG ATATGTTCAC AGGACAGAAC 
CCTAAAGACC ATGAGACATG GGAAACTCGC TACATGCTTT TACTGTGGCT GTCTGTGACC 
TGCTTGATCC CTTTTGATTT TTCACGCCTT GATGGGAACC TGCCCACTCA ATCTGGAGAA 
ACACGGATGC CCATAATGGA CCGAATTCTG CAAATAGCAC AGTCTTACTT GGTGGTCAGT 
GACAAGGCGC GAGATGCGGC TGCTGTCCTT GTGTCCAAGT TTATCACACG TCCTGATGTC 
AAGCAGAGAA AGATGGCCAG CTTTCTGGAC TGGAGCCTTT GTACCTTGGC CCACTCTTCC 
TTCCAGACCA TTGACGGTGT CATTACCATG GATGGGATGT TGCAGGCCCT GGCTCAAATA 
TTTAAGCATG GAAAACGTGA AGACTGTCTA CCCTATGCCA ACACTGTGCT CCAGTGCCTT 
GATGGATGTA GACTCCCTGA GAGCAGTCAC ACCTTACTGC GGAAGCTTGG TGTGAAGCTT 
GTCCAGAGGC TGGGGCTGAC TTTCCTGAAG CCAAAGGTGG CCACGTGGAG GTATCAGCGT 
GGCTGTCGGT CTTTGGCTGC CAACCTGCAG CTCCGTGCTC CGGGTAAGAG TGAACAGAAG 
CTGCTGTCTG ATAGTTTGAC CTCTGATGGT GATGAAGACT ATGATGTCCC AGAGGGGGTG 
GAAACTGTGA TAGAGCAACT GCTGGTTGGG CTGAAGGACA AGGACACGAT AGTGCGGTGG 
TCTGCAGCTA AAGGAATTGG TAGGATGGCT GGAAGGCTTC CCAGAGAGCT GGCAGATGAT 
GTGGTGGGGT CCGTGTTGGA CTGTTTCAGC TTTCAGGAGA CAGATAAGGC ATGGCATGGT 
GGATGTCTGG CACTGGCAGA GCTGGGTAGA CGAGGCCTGT TGCTGCCATC GAGACTCTCA 
GAGGTTGTCA CAGTGATCCT GAAGGCCCTT ACCTACGATG AGAAGCGGGG TGCCTGCAGC 
GTGGGGGCCA ACGTCAGGGA TGCTGCCTGC TATGTGTGCT GGGCTTTTGC ACGTGCCTAC 
GAGCCTCAGG AGTTGACACC TTTCGTGACT GCCATTTCAA GTGCCCTGGT CATTGCTGCT 
GTATTTGACC GAAACGTGAA CTGCAGAAGA GCAGCCTCTG CTGCCTTCCA GGAGAACGTG 
GGGAGACAGG GTACTTTTCC TCATGGAATT GATATTTTGA CTACAGCAGA CTATTTTGCA 
GTTGGTAACA TTTCTAACTG TTTCCTGATT ATAAGCGTGT TTATTGCTGG CTTCCAGGAA 
TACACCAAGC CAATGATCGA CCACCTGGTC TCCATGAAAA TCAACCACTG GGATGGGGCC 
ATCCGAGAAC TGTCTGCAAA GGCACTGCAC AACCTGACTT CACTAGTACC CGAGTACATG 
GCCACTCACG TATTCCCAGC ACTACTGCTG ATGACACAGA GTCCAGATCT ACACACGAGA 
CATGGTGCCA TCCTTGCCTG TGCAGAGGTC ACCTATGCCC TGTACGAGCT GGCCGCACAG 
AGTAACAGGC TTGTCACAGA CTATCTGGAT GAGAAGGCAG TGCAAAGCCT GAAGCAGATC 
CACCAGCAGC TTTGTGATCG CCATCTCTAC CGGGGTCTGG GAGGAGAGCT TATGAGACAA 
GCAGTGTGCA TCTTAATAGA GAAATTGTCA CTTTCCAGAA TGCCCTTTAA AGGTGACACC 
GTTATTGAGG GATGGCAGTG GCTGATAAAT GACACTCTCA GGAGCCTTCA CCTTGTTTCA 
AGCCATTCCA GAGAACAGAT CAAGGAGGTG GCTGTCTCTG CCCTGGCTGC CGTCTGCAAT 
GAATACTACA AGAAGGAGCC TGGGGAGGCA GGCTCTACCA TCACAGAGGA GCTGATTCCA 
CAGTACCTTG CTGAACTCCG AAGCCCAGAG GAGATGACCC GTTGTGGCTT TTCCTCAGCC 
TTGGGTGCCC TCCCGTGTTT CCTTCTCAGG GGCCATCTGC AGCAGGTTCT CTCAGGTCTG 
AGAAGAGTCA CCTGTACTAC TTCTCCCATG GATGTGAGCT TTGCTGAGGC CAGGAGAGAC 
GGTTTGAAAG CAATCTCGAG AATTTGCCAG ACAGTTGGTG TGAACACACA GGGACCTCCA 
GATGAAGTCA TATGCCAAGA GAACATCTCT GAGGTTTATG CTGCATTGCT GGGCTGCATG 
AGTGATTACA CCACAGACAG CAGAGGCGAT GTGGGAGCCT GGGTTCGTGA GGCTTCCATG 
ACCAGCCTCA TGGATTTGAT GCTTTTGCTG GCACAGACTG AGCCAGTGCT GATTGAGGCC 
CACATCTGTG AGCGTGTCAT GTGTTGCGTT GCCCAGCAGG CAAGTGAGAA GATTGATCGG 
TTCCGTGCTC ATGCTGCCCG GGTATTCCTG ACTCTCTTGC ACTTCGACAG CCCTCCCATT 
CCCCATGTTC CCCACCGCAA GGAACTGGAA AGTCTGTTTC CCAGGTCTGA TGTGGCCACT 
GTGAACTGGA ATGCACCTTC ACAGGCCTTC CCTCTTATCA CCCAGCTCTT GGGACTGCCT 
ACCTATAGAT ACCATGTCCT GCTGGGGCTG GCCGTGTCCG TGGGTGGCTT GACAGAGTCT 
ACGGTCAGGC ATTCTACACA GAGCCTCTTC GAGTACATGA AGGGGATTCA GAAGGATGCT 
CAGGTCCTGG AGAGCTTCAG TGAGACCCTC CTAAAGGTCT TTGAGGACAA CCTCTTGAAT 
GACAGAGTGT CTGTATCGCT GCTGAAAATG TTGGACCAGC TGTTGGCCAA TGGGTGCTTT 
GACATCTTTA CTGCCGAGGA GAACCACCCC TTTTGTGTGA AGTTGCTCAC ACTTTGTAAG 
GAAGAAATCA AGAAGTCAAA AGACATCCAG AAACTTCGGT CAAGCATTGC AGTGCTCTGT 
GGGATGGTGC AGTTCAGTGG TGATGTGAGA AAGAAGGTTC TTCTGCAGCT CTTCCTCCTC 
CTTGGCCACC CTTTTCCTGT GGTGCTGATG GCTTTTCCTC CTGTTGACCT GCTGTTCTCA 
TTCCAGATCC GGAAGAGCAC AGCAAGCCAG GTATATGAGA TGGTGCTTAC CTATAGTGAC 
TTGGTAGATG CTGAGGTGCT TGATGAGGTC ATGTCTGTGC TCAGTGACAC AGCCTGGGAC 
TCAGAGCTTC CCGTTGTAAG AGGACAGCGG AATCACCTGT GTGACCTCCT TGGTGTGCCC 
AGACCCCAAC TGGTTCCAAA GCCTGTTCCA GGAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006247923.1
CDS	111..3749
Translation	MVLSNEPAASAAEEEVEDDALVRASTLEAFGESAETRALLRSLPAVHRERAAREVAEERFRVIMDKYQEQPHLLDPHLEWMMNSLLDIVQDETSLPDLVHLAFKFLYIITKVRGYKVFLRLFPHEVADVQPVLDMFTGQNPKDHETWETRYMLLLWLSVTCLIPFDFSRLDGNLPTQSGETRMPIMDRILQIAQSYLVVSDKARDAAAVLVSKFITRPDVKQRKMASFLDWSLCTLAHSSFQTIDGVITMDGMLQALAQIFKHGKREDCLPYANTVLQCLDGCRLPESSHTLLRKLGVKLVQRLGLTFLKPKVATWRYQRGCRSLAANLQLRAPGKSEQKLLSDSLTSDGDEDYDVPEGVETVIEQLLVGLKDKDTIVRWSAAKGIGRMAGRLPRELADDVVGSVLDCFSFQETDKAWHGGCLALAELGRRGLLLPSRLSEVVTVILKALTYDEKRGACSVGANVRDAACYVCWAFARAYEPQELTPFVTAISSALVIAAVFDRNVNCRRAASAAFQENVGRQGTFPHGIDILTTADYFAVGNISNCFLIISVFIAGFQEYTKPMIDHLVSMKINHWDGAIRELSAKALHNLTSLVPEYMATHVFPALLLMTQSPDLHTRHGAILACAEVTYALYELAAQSNRLVTDYLDEKAVQSLKQIHQQLCDRHLYRGLGGELMRQAVCILIEKLSLSRMPFKGDTVIEGWQWLINDTLRSLHLVSSHSREQIKEVAVSALAAVCNEYYKKEPGEAGSTITEELIPQYLAELRSPEEMTRCGFSSALGALPCFLLRGHLQQVLSGLRRVTCTTSPMDVSFAEARRDGLKAISRICQTVGVNTQGPPDEVICQENISEVYAALLGCMSDYTTDSRGDVGAWVREASMTSLMDLMLLLAQTEPVLIEAHICERVMCCVAQQASEKIDRFRAHAARVFLTLLHFDSPPIPHVPHRKELESLFPRSDVATVNWNAPSQAFPLITQLLGLPTYRYHVLLGLAVSVGGLTESTVRHSTQSLFEYMKGIQKDAQVLESFSETLLKVFEDNLLNDRVSVSLLKMLDQLLANGCFDIFTAEENHPFCVKLLTLCKEEIKKSKDIQKLRSSIAVLCGMVQFSGDVRKKVLLQLFLLLGHPFPVVLMAFPPVDLLFSFQIRKSTASQVYEMVLTYSDLVDAEVLDEVMSVLSDTAWDSELPVVRGQRNHLCDLLGVPRPQLVPKPVPGS

Target ORF information:

RefSeq Version	XM_006247861.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus tubulin folding cofactor D (Tbcd), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006247861.3

ORF Insert Sequence:

ATGGTACTGA GCAACGAGCC GGCTGCGAGC GCCGCGGAGG AGGAGGTGGA GGATGATGCG 
CTTGTCCGCG CCTCCACCCT GGAAGCGTTC GGCGAGAGTG CGGAGACCCG CGCGCTGCTC 
CGCAGCTTGC CGGCGGTGCA CCGCGAGCGC GCGGCCCGGG AGGTGGCGGA GGAGCGGTTC 
CGCGTAATTA TGGACAAATA CCAGGAGCAG CCCCACCTCC TGGACCCACA CCTCGAGTGG 
ATGATGAACT CACTGTTGGA CATAGTACAG GATGAGACAT CTCTGCCCGA CCTTGTACAT 
CTGGCTTTTA AATTTCTGTA TATCATCACC AAGGTTCGTG GATATAAAGT ATTTCTGCGT 
CTGTTTCCCC ATGAAGTAGC TGATGTTCAG CCTGTGTTAG ATATGTTCAC AGGACAGAAC 
CCTAAAGACC ATGAGACATG GGAAACTCGC TACATGCTTT TACTGTGGCT GTCTGTGACC 
TGCTTGATCC CTTTTGATTT TTCACGCCTT GATGGGAACC TGCCCACTCA ATCTGGAGAA 
ACACGGATGC CCATAATGGA CCGAATTCTG CAAATAGCAC AGTCTTACTT GGTGGTCAGT 
GACAAGGCGC GAGATGCGGC TGCTGTCCTT GTGTCCAAGT TTATCACACG TCCTGATGTC 
AAGCAGAGAA AGATGGCCAG CTTTCTGGAC TGGAGCCTTT GTACCTTGGC CCACTCTTCC 
TTCCAGACCA TTGACGGTGT CATTACCATG GATGGGATGT TGCAGGCCCT GGCTCAAATA 
TTTAAGCATG GAAAACGTGA AGACTGTCTA CCCTATGCCA ACACTGTGCT CCAGTGCCTT 
GATGGATGTA GACTCCCTGA GAGCAGTCAC ACCTTACTGC GGAAGCTTGG TGTGAAGCTT 
GTCCAGAGGC TGGGGCTGAC TTTCCTGAAG CCAAAGGTGG CCACGTGGAG GTATCAGCGT 
GGCTGTCGGT CTTTGGCTGC CAACCTGCAG CTCCGTGCTC CGGGTAAGAG TGAACAGAAG 
CTGCTGTCTG ATAGTTTGAC CTCTGATGGT GATGAAGACT ATGATGTCCC AGAGGGGGTG 
GAAACTGTGA TAGAGCAACT GCTGGTTGGG CTGAAGGACA AGGACACGAT AGTGCGGTGG 
TCTGCAGCTA AAGGAATTGG TAGGATGGCT GGAAGGCTTC CCAGAGAGCT GGCAGATGAT 
GTGGTGGGGT CCGTGTTGGA CTGTTTCAGC TTTCAGGAGA CAGATAAGGC ATGGCATGGT 
GGATGTCTGG CACTGGCAGA GCTGGGTAGA CGAGGCCTGT TGCTGCCATC GAGACTCTCA 
GAGGTTGTCA CAGTGATCCT GAAGGCCCTT ACCTACGATG AGAAGCGGGG TGCCTGCAGC 
GTGGGGGCCA ACGTCAGGGA TGCTGCCTGC TATGTGTGCT GGGCTTTTGC ACGTGCCTAC 
GAGCCTCAGG AGTTGACACC TTTCGTGACT GCCATTTCAA GTGCCCTGGT CATTGCTGCT 
GTATTTGACC GAAACGTGAA CTGCAGAAGA GCAGCCTCTG CTGCCTTCCA GGAGAACGTG 
GGGAGACAGG GTACTTTTCC TCATGGAATT GATATTTTGA CTACAGCAGA CTATTTTGCA 
GTTGGTAACA TTTCTAACTG TTTCCTGATT ATAAGCGTGT TTATTGCTGG CTTCCAGGAA 
TACACCAAGC CAATGATCGA CCACCTGGTC TCCATGAAAA TCAACCACTG GGATGGGGCC 
ATCCGAGAAC TGTCTGCAAA GGCACTGCAC AACCTGACTT CACTAGTACC CGAGTACATG 
GCCACTCACG TATTCCCAGC ACTACTGCTG ATGACACAGA GTCCAGATCT ACACACGAGA 
CATGGTGCCA TCCTTGCCTG TGCAGAGGTC ACCTATGCCC TGTACGAGCT GGCCGCACAG 
AGTAACAGGC TTGTCACAGA CTATCTGGAT GAGAAGGCAG TGCAAAGCCT GAAGCAGATC 
CACCAGCAGC TTTGTGATCG CCATCTCTAC CGGGGTCTGG GAGGAGAGCT TATGAGACAA 
GCAGTGTGCA TCTTAATAGA GAAATTGTCA CTTTCCAGAA TGCCCTTTAA AGGTGACACC 
GTTATTGAGG GATGGCAGTG GCTGATAAAT GACACTCTCA GGAGCCTTCA CCTTGTTTCA 
AGCCATTCCA GAGAACAGAT CAAGGAGGTG GCTGTCTCTG CCCTGGCTGC CGTCTGCAAT 
GAATACTACA AGAAGGAGCC TGGGGAGGCA GGCTCTACCA TCACAGAGGA GCTGATTCCA 
CAGTACCTTG CTGAACTCCG AAGCCCAGAG GAGATGACCC GTTGTGGCTT TTCCTCAGCC 
TTGGGTGCCC TCCCGTGTTT CCTTCTCAGG GGCCATCTGC AGCAGGTTCT CTCAGGTCTG 
AGAAGAGTCA CCTGTACTAC TTCTCCCATG GATGTGAGCT TTGCTGAGGC CAGGAGAGAC 
GGTTTGAAAG CAATCTCGAG AATTTGCCAG ACAGTTGGTG TGAACACACA GGGACCTCCA 
GATGAAGTCA TATGCCAAGA GAACATCTCT GAGGTTTATG CTGCATTGCT GGGCTGCATG 
AGTGATTACA CCACAGACAG CAGAGGCGAT GTGGGAGCCT GGGTTCGTGA GGCTTCCATG 
ACCAGCCTCA TGGATTTGAT GCTTTTGCTG GCACAGACTG AGCCAGTGCT GATTGAGGCC 
CACATCTGTG AGCGTGTCAT GTGTTGCGTT GCCCAGCAGG CAAGTGAGAA GATTGATCGG 
TTCCGTGCTC ATGCTGCCCG GGTATTCCTG ACTCTCTTGC ACTTCGACAG CCCTCCCATT 
CCCCATGTTC CCCACCGCAA GGAACTGGAA AGTCTGTTTC CCAGGTCTGA TGTGGCCACT 
GTGAACTGGA ATGCACCTTC ACAGGCCTTC CCTCTTATCA CCCAGCTCTT GGGACTGCCT 
ACCTATAGAT ACCATGTCCT GCTGGGGCTG GCCGTGTCCG TGGGTGGCTT GACAGAGTCT 
ACGGTCAGGC ATTCTACACA GAGCCTCTTC GAGTACATGA AGGGGATTCA GAAGGATGCT 
CAGGTCCTGG AGAGCTTCAG TGAGACCCTC CTAAAGGTCT TTGAGGACAA CCTCTTGAAT 
GACAGAGTGT CTGTATCGCT GCTGAAAATG TTGGACCAGC TGTTGGCCAA TGGGTGCTTT 
GACATCTTTA CTGCCGAGGA GAACCACCCC TTTTGTGTGA AGTTGCTCAC ACTTTGTAAG 
GAAGAAATCA AGAAGTCAAA AGACATCCAG AAACTTCGGT CAAGCATTGC AGTGCTCTGT 
GGGATGGTGC AGTTCAGTGG TGATGTGAGA AAGAAGGTTC TTCTGCAGCT CTTCCTCCTC 
CTTGGCCACC CTTTTCCTGT GGTGCTGATG GCTTTTCCTC CTGTTGACCT GCTGTTCTCA 
TTCCAGATCC GGAAGAGCAC AGCAAGCCAG GTATATGAGA TGGTGCTTAC CTATAGTGAC 
TTGGTAGATG CTGAGGTGCT TGATGAGGTC ATGTCTGTGC TCAGTGACAC AGCCTGGGAC 
TCAGAGCTTC CCGTTGTAAG AGGACAGCGG AATCACCTGT GTGACCTCCT TGGTGTGCCC 
AGACCCCAAC TGGTTCCAAA GCCTGTTCCA GGAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tbcd ( XM_006247864.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006247926.1 Rattus norvegicus tubulin folding cofactor D (Tbcd), transcript variant X2, mRNA.

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CloneID	ORa34013
Clone ID Related Accession (Same CDS sequence)	XM_006247864.3
Accession Version	XM_006247864.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3429bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	tubulin-specific chaperone D isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006247864.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTACTGA GCAACGAGCC GGCTGCGAGC GCCGCGGAGG AGGAGGTGGA GGATGATGCG 
CTTGTCCGCG CCTCCACCCT GGAAGCGTTC GGCGAGAGTG CGGAGACCCG CGCGCTGCTC 
CGCAGCTTGC CGGCGGTGCA CCGCGAGCGC GCGGCCCGGG AGGTGGCGGA GGAGCGGTTC 
CGCGTAATTA TGGACAAATA CCAGGAGCAG CCCCACCTCC TGGACCCACA CCTCGAGTGG 
ATGATGAACT CACTGTTGGA CATAGTACAG GATGAGACAT CTCTGCCCGA CCTTGTACAT 
CTGGCTTTTA AATTTCTGTA TATCATCACC AAGGTTCGTG GATATAAAGT ATTTCTGCGT 
CTGTTTCCCC ATGAAGTAGC TGATGTTCAG CCTGTGTTAG ATATGTTCAC AGGACAGAAC 
CCTAAAGACC ATGAGACATG GGAAACTCGC TACATGCTTT TACTGTGGCT GTCTGTGACC 
TGCTTGATCC CTTTTGATTT TTCACGCCTT GATGGGAACC TGCCCACTCA ATCTGGAGAA 
ACACGGATGC CCATAATGGA CCGAATTCTG CAAATAGCAC AGTCTTACTT GGTGGTCAGT 
GACAAGGCGC GAGATGCGGC TGCTGTCCTT GTGTCCAAGT TTATCACACG TCCTGATGTC 
AAGCAGAGAA AGATGGCCAG CTTTCTGGAC TGGAGCCTTT GTACCTTGGC CCACTCTTCC 
TTCCAGACCA TTGACGGTGT CATTACCATG GATGGGATGT TGCAGGCCCT GGCTCAAATA 
TTTAAGCATG GAAAACGTGA AGACTGTCTA CCCTATGCCA ACACTGTGCT CCAGTGCCTT 
GATGGATGTA GACTCCCTGA GAGCAGTCAC ACCTTACTGC GGAAGCTTGG TGTGAAGCTT 
GTCCAGAGGC TGGGGCTGAC TTTCCTGAAG CCAAAGGTGG CCACGTGGAG GTATCAGCGT 
GGCTGTCGGT CTTTGGCTGC CAACCTGCAG CTCCGTGCTC CGGGTAAGAG TGAACAGAAG 
CTGCTGTCTG ATAGTTTGAC CTCTGATGGT GATGAAGACT ATGATGTCCC AGAGGGGGTG 
GAAACTGTGA TAGAGCAACT GCTGGTTGGG CTGAAGGACA AGGACACGAT AGTGCGGTGG 
TCTGCAGCTA AAGGAATTGG TAGGATGGCT GGAAGGCTTC CCAGAGAGCT GGCAGATGAT 
GTGGTGGGGT CCGTGTTGGA CTGTTTCAGC TTTCAGGAGA CAGATAAGGC ATGGCATGGT 
GGATGTCTGG CACTGGCAGA GCTGGGTAGA CGAGGCCTGT TGCTGCCATC GAGACTCTCA 
GAGGTTGTCA CAGTGATCCT GAAGGCCCTT ACCTACGATG AGAAGCGGGG TGCCTGCAGC 
GTGGGGGCCA ACGTCAGGGA TGCTGCCTGC TATGTGTGCT GGGCTTTTGC ACGTGCCTAC 
GAGCCTCAGG AGTTGACACC TTTCGTGACT GCCATTTCAA GTGCCCTGGT CATTGCTGCT 
GTATTTGACC GAAACGTGAA CTGCAGAAGA GCAGCCTCTG CTGCCTTCCA GGAGAACGTG 
GGGAGACAGG GTACTTTTCC TCATGGAATT GATATTTTGA CTACAGCAGA CTATTTTGCA 
GTTGGTAACA TTTCTAACTG TTTCCTGATT ATAAGCGTGT TTATTGCTGG CTTCCAGGAA 
TACACCAAGC CAATGATCGA CCACCTGGTC TCCATGAAAA TCAACCACTG GGATGGGGCC 
ATCCGAGAAC TGTCTGCAAA GGCACTGCAC AACCTGACTT CACTAGTACC CGAGTACATG 
GCCACTCACG TATTCCCAGC ACTACTGCTG ATGACACAGA GTCCAGATCT ACACACGAGA 
CATGGTGCCA TCCTTGCCTG TGCAGAGGTC ACCTATGCCC TGTACGAGCT GGCCGCACAG 
AGTAACAGGC TTGTCACAGA CTATCTGGAT GAGAAGGCAG TGCAAAGCCT GAAGCAGATC 
CACCAGCAGC TTTGTGATCG CCATCTCTAC CGGGGTCTGG GAGGAGAGCT TATGAGACAA 
GCAGTGTGCA TCTTAATAGA GAAATTGTCA CTTTCCAGAA TGCCCTTTAA AGGTGACACC 
GTTATTGAGG GATGGCAGTG GCTGATAAAT GACACTCTCA GGAGCCTTCA CCTTGTTTCA 
AGCCATTCCA GAGAACAGAT CAAGGAGGTG GCTGTCTCTG CCCTGGCTGC CGTCTGCAAT 
GAATACTACA AGAAGGAGCC TGGGGAGGCA GGCTCTACCA TCACAGAGGA GCTGATTCCA 
CAGTACCTTG CTGAACTCCG AAGCCCAGAG GAGATGACCC GTTGTGGCTT TTCCTCAGCC 
TTGGGTGCCC TCCCGTGTTT CCTTCTCAGG GGCCATCTGC AGCAGGTTCT CTCAGGTCTG 
AGAAGAGTCA CCTGTACTAC TTCTCCCATG GATGTGAGCT TTGCTGAGGC CAGGAGAGAC 
GGTTTGAAAG CAATCTCGAG AATTTGCCAG ACAGTTGGTG TGAACACACA GGGACCTCCA 
GATGAAGTCA TATGCCAAGA GAACATCTCT GAGGTTTATG CTGCATTGCT GGGCTGCATG 
AGTGATTACA CCACAGACAG CAGAGGCGAT GTGGGAGCCT GGGTTCGTGA GGCTTCCATG 
ACCAGCCTCA TGGATTTGAT GCTTTTGCTG GCACAGACTG AGCCAGTGCT GATTGAGGCC 
CACATCTGTG AGCGTGTCAT GTGTTGCGTT GCCCAGCAGG CAAGTGAGAA GATTGATCGG 
TTCCGTGCTC ATGCTGCCCG GGTATTCCTG ACTCTCTTGC ACTTCGACAG CCCTCCCATT 
CCCCATGTTC CCCACCGCAA GGAACTGGAA AGTCTGTTTC CCAGGTCTGA TGTGGCCACT 
GTGAACTGGA ATGCACCTTC ACAGGCCTTC CCTCTTATCA CCCAGCTCTT GGGACTGCCT 
ACCTATAGAT ACCATGTCCT GCTGGGGCTG GCCGTGTCCG TGGGTGGCTT GACAGAGTCT 
ACGGTCAGGC ATTCTACACA GAGCCTCTTC GAGTACATGA AGGGGATTCA GAAGGATGCT 
CAGGTCCTGG AGAGCTTCAG TGAGACCCTC CTAAAGGTCT TTGAGGACAA CCTCTTGAAT 
GACAGAGTGT CTGTATCGCT GCTGAAAATG TTGGACCAGC TGTTGGCCAA TGGGTGCTTT 
GACATCTTTA CTGCCGAGGA GAACCACCCC TTTTGTGTGA AGTTGCTCAC ACTTTGTAAG 
GAAGAAATCA AGAAGTCAAA AGACATCCAG AAACTTCGGT CAAGCATTGC AGTGCTCTGT 
GGGATGGTGC AGTTCAGTGG TGATGTGAGA AAGAAGGTTC TTCTGCAGCT CTTCCTCCTC 
CTTGGCCACC CTTTTCCTGT GGGACTCAGA GCTTCCCGTT GTAAGAGGAC AGCGGAATCA 
CCTGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006247926.1
CDS	110..3538
Translation	MVLSNEPAASAAEEEVEDDALVRASTLEAFGESAETRALLRSLPAVHRERAAREVAEERFRVIMDKYQEQPHLLDPHLEWMMNSLLDIVQDETSLPDLVHLAFKFLYIITKVRGYKVFLRLFPHEVADVQPVLDMFTGQNPKDHETWETRYMLLLWLSVTCLIPFDFSRLDGNLPTQSGETRMPIMDRILQIAQSYLVVSDKARDAAAVLVSKFITRPDVKQRKMASFLDWSLCTLAHSSFQTIDGVITMDGMLQALAQIFKHGKREDCLPYANTVLQCLDGCRLPESSHTLLRKLGVKLVQRLGLTFLKPKVATWRYQRGCRSLAANLQLRAPGKSEQKLLSDSLTSDGDEDYDVPEGVETVIEQLLVGLKDKDTIVRWSAAKGIGRMAGRLPRELADDVVGSVLDCFSFQETDKAWHGGCLALAELGRRGLLLPSRLSEVVTVILKALTYDEKRGACSVGANVRDAACYVCWAFARAYEPQELTPFVTAISSALVIAAVFDRNVNCRRAASAAFQENVGRQGTFPHGIDILTTADYFAVGNISNCFLIISVFIAGFQEYTKPMIDHLVSMKINHWDGAIRELSAKALHNLTSLVPEYMATHVFPALLLMTQSPDLHTRHGAILACAEVTYALYELAAQSNRLVTDYLDEKAVQSLKQIHQQLCDRHLYRGLGGELMRQAVCILIEKLSLSRMPFKGDTVIEGWQWLINDTLRSLHLVSSHSREQIKEVAVSALAAVCNEYYKKEPGEAGSTITEELIPQYLAELRSPEEMTRCGFSSALGALPCFLLRGHLQQVLSGLRRVTCTTSPMDVSFAEARRDGLKAISRICQTVGVNTQGPPDEVICQENISEVYAALLGCMSDYTTDSRGDVGAWVREASMTSLMDLMLLLAQTEPVLIEAHICERVMCCVAQQASEKIDRFRAHAARVFLTLLHFDSPPIPHVPHRKELESLFPRSDVATVNWNAPSQAFPLITQLLGLPTYRYHVLLGLAVSVGGLTESTVRHSTQSLFEYMKGIQKDAQVLESFSETLLKVFEDNLLNDRVSVSLLKMLDQLLANGCFDIFTAEENHPFCVKLLTLCKEEIKKSKDIQKLRSSIAVLCGMVQFSGDVRKKVLLQLFLLLGHPFPVGLRASRCKRTAESPV

Target ORF information:

RefSeq Version	XM_006247864.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus tubulin folding cofactor D (Tbcd), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006247864.3

ORF Insert Sequence:

ATGGTACTGA GCAACGAGCC GGCTGCGAGC GCCGCGGAGG AGGAGGTGGA GGATGATGCG 
CTTGTCCGCG CCTCCACCCT GGAAGCGTTC GGCGAGAGTG CGGAGACCCG CGCGCTGCTC 
CGCAGCTTGC CGGCGGTGCA CCGCGAGCGC GCGGCCCGGG AGGTGGCGGA GGAGCGGTTC 
CGCGTAATTA TGGACAAATA CCAGGAGCAG CCCCACCTCC TGGACCCACA CCTCGAGTGG 
ATGATGAACT CACTGTTGGA CATAGTACAG GATGAGACAT CTCTGCCCGA CCTTGTACAT 
CTGGCTTTTA AATTTCTGTA TATCATCACC AAGGTTCGTG GATATAAAGT ATTTCTGCGT 
CTGTTTCCCC ATGAAGTAGC TGATGTTCAG CCTGTGTTAG ATATGTTCAC AGGACAGAAC 
CCTAAAGACC ATGAGACATG GGAAACTCGC TACATGCTTT TACTGTGGCT GTCTGTGACC 
TGCTTGATCC CTTTTGATTT TTCACGCCTT GATGGGAACC TGCCCACTCA ATCTGGAGAA 
ACACGGATGC CCATAATGGA CCGAATTCTG CAAATAGCAC AGTCTTACTT GGTGGTCAGT 
GACAAGGCGC GAGATGCGGC TGCTGTCCTT GTGTCCAAGT TTATCACACG TCCTGATGTC 
AAGCAGAGAA AGATGGCCAG CTTTCTGGAC TGGAGCCTTT GTACCTTGGC CCACTCTTCC 
TTCCAGACCA TTGACGGTGT CATTACCATG GATGGGATGT TGCAGGCCCT GGCTCAAATA 
TTTAAGCATG GAAAACGTGA AGACTGTCTA CCCTATGCCA ACACTGTGCT CCAGTGCCTT 
GATGGATGTA GACTCCCTGA GAGCAGTCAC ACCTTACTGC GGAAGCTTGG TGTGAAGCTT 
GTCCAGAGGC TGGGGCTGAC TTTCCTGAAG CCAAAGGTGG CCACGTGGAG GTATCAGCGT 
GGCTGTCGGT CTTTGGCTGC CAACCTGCAG CTCCGTGCTC CGGGTAAGAG TGAACAGAAG 
CTGCTGTCTG ATAGTTTGAC CTCTGATGGT GATGAAGACT ATGATGTCCC AGAGGGGGTG 
GAAACTGTGA TAGAGCAACT GCTGGTTGGG CTGAAGGACA AGGACACGAT AGTGCGGTGG 
TCTGCAGCTA AAGGAATTGG TAGGATGGCT GGAAGGCTTC CCAGAGAGCT GGCAGATGAT 
GTGGTGGGGT CCGTGTTGGA CTGTTTCAGC TTTCAGGAGA CAGATAAGGC ATGGCATGGT 
GGATGTCTGG CACTGGCAGA GCTGGGTAGA CGAGGCCTGT TGCTGCCATC GAGACTCTCA 
GAGGTTGTCA CAGTGATCCT GAAGGCCCTT ACCTACGATG AGAAGCGGGG TGCCTGCAGC 
GTGGGGGCCA ACGTCAGGGA TGCTGCCTGC TATGTGTGCT GGGCTTTTGC ACGTGCCTAC 
GAGCCTCAGG AGTTGACACC TTTCGTGACT GCCATTTCAA GTGCCCTGGT CATTGCTGCT 
GTATTTGACC GAAACGTGAA CTGCAGAAGA GCAGCCTCTG CTGCCTTCCA GGAGAACGTG 
GGGAGACAGG GTACTTTTCC TCATGGAATT GATATTTTGA CTACAGCAGA CTATTTTGCA 
GTTGGTAACA TTTCTAACTG TTTCCTGATT ATAAGCGTGT TTATTGCTGG CTTCCAGGAA 
TACACCAAGC CAATGATCGA CCACCTGGTC TCCATGAAAA TCAACCACTG GGATGGGGCC 
ATCCGAGAAC TGTCTGCAAA GGCACTGCAC AACCTGACTT CACTAGTACC CGAGTACATG 
GCCACTCACG TATTCCCAGC ACTACTGCTG ATGACACAGA GTCCAGATCT ACACACGAGA 
CATGGTGCCA TCCTTGCCTG TGCAGAGGTC ACCTATGCCC TGTACGAGCT GGCCGCACAG 
AGTAACAGGC TTGTCACAGA CTATCTGGAT GAGAAGGCAG TGCAAAGCCT GAAGCAGATC 
CACCAGCAGC TTTGTGATCG CCATCTCTAC CGGGGTCTGG GAGGAGAGCT TATGAGACAA 
GCAGTGTGCA TCTTAATAGA GAAATTGTCA CTTTCCAGAA TGCCCTTTAA AGGTGACACC 
GTTATTGAGG GATGGCAGTG GCTGATAAAT GACACTCTCA GGAGCCTTCA CCTTGTTTCA 
AGCCATTCCA GAGAACAGAT CAAGGAGGTG GCTGTCTCTG CCCTGGCTGC CGTCTGCAAT 
GAATACTACA AGAAGGAGCC TGGGGAGGCA GGCTCTACCA TCACAGAGGA GCTGATTCCA 
CAGTACCTTG CTGAACTCCG AAGCCCAGAG GAGATGACCC GTTGTGGCTT TTCCTCAGCC 
TTGGGTGCCC TCCCGTGTTT CCTTCTCAGG GGCCATCTGC AGCAGGTTCT CTCAGGTCTG 
AGAAGAGTCA CCTGTACTAC TTCTCCCATG GATGTGAGCT TTGCTGAGGC CAGGAGAGAC 
GGTTTGAAAG CAATCTCGAG AATTTGCCAG ACAGTTGGTG TGAACACACA GGGACCTCCA 
GATGAAGTCA TATGCCAAGA GAACATCTCT GAGGTTTATG CTGCATTGCT GGGCTGCATG 
AGTGATTACA CCACAGACAG CAGAGGCGAT GTGGGAGCCT GGGTTCGTGA GGCTTCCATG 
ACCAGCCTCA TGGATTTGAT GCTTTTGCTG GCACAGACTG AGCCAGTGCT GATTGAGGCC 
CACATCTGTG AGCGTGTCAT GTGTTGCGTT GCCCAGCAGG CAAGTGAGAA GATTGATCGG 
TTCCGTGCTC ATGCTGCCCG GGTATTCCTG ACTCTCTTGC ACTTCGACAG CCCTCCCATT 
CCCCATGTTC CCCACCGCAA GGAACTGGAA AGTCTGTTTC CCAGGTCTGA TGTGGCCACT 
GTGAACTGGA ATGCACCTTC ACAGGCCTTC CCTCTTATCA CCCAGCTCTT GGGACTGCCT 
ACCTATAGAT ACCATGTCCT GCTGGGGCTG GCCGTGTCCG TGGGTGGCTT GACAGAGTCT 
ACGGTCAGGC ATTCTACACA GAGCCTCTTC GAGTACATGA AGGGGATTCA GAAGGATGCT 
CAGGTCCTGG AGAGCTTCAG TGAGACCCTC CTAAAGGTCT TTGAGGACAA CCTCTTGAAT 
GACAGAGTGT CTGTATCGCT GCTGAAAATG TTGGACCAGC TGTTGGCCAA TGGGTGCTTT 
GACATCTTTA CTGCCGAGGA GAACCACCCC TTTTGTGTGA AGTTGCTCAC ACTTTGTAAG 
GAAGAAATCA AGAAGTCAAA AGACATCCAG AAACTTCGGT CAAGCATTGC AGTGCTCTGT 
GGGATGGTGC AGTTCAGTGG TGATGTGAGA AAGAAGGTTC TTCTGCAGCT CTTCCTCCTC 
CTTGGCCACC CTTTTCCTGT GGGACTCAGA GCTTCCCGTT GTAAGAGGAC AGCGGAATCA 
CCTGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Infantile neurodegenerative disorder associated with mutations in TBCD, an essential gene in the tubulin heterodimer assembly pathway.
Human molecular genetics25(21)4635-4648(2016 11)
Edvardson S,Tian G,Cullen H,Vanyai H,Ngo L,Bhat S,Aran A,Daana M,Da'amseh N,Abu-Libdeh B,Cowan NJ,Heng JI,Elpeleg O

Biallelic Mutations in TBCD, Encoding the Tubulin Folding Cofactor D, Perturb Microtubule Dynamics and Cause Early-Onset Encephalopathy.
American journal of human genetics99(4)962-973(2016 Oct)
Flex E,Niceta M,Cecchetti S,Thiffault I,Au MG,Capuano A,Piermarini E,Ivanova AA,Francis JW,Chillemi G,Chandramouli B,Carpentieri G,Haaxma CA,Ciolfi A,Pizzi S,Douglas GV,Levine K,Sferra A,Dentici ML,Pfundt RR,Le Pichon JB,Farrow E,Baas F,Piemonte F,Dallapiccola B,Graham JM Jr,Saunders CJ,Bertini E,Kahn RA,Koolen DA,Tartaglia M

Biallelic TBCD Mutations Cause Early-Onset Neurodegenerative Encephalopathy.
American journal of human genetics99(4)950-961(2016 Oct)
Miyake N,Fukai R,Ohba C,Chihara T,Miura M,Shimizu H,Kakita A,Imagawa E,Shiina M,Ogata K,Okuno-Yuguchi J,Fueki N,Ogiso Y,Suzumura H,Watabe Y,Imataka G,Leong HY,Fattal-Valevski A,Kramer U,Miyatake S,Kato M,Okamoto N,Sato Y,Mitsuhashi S,Nishino I,Kaneko N,Nishiyama A,Tamura T,Mizuguchi T,Nakashima M,Tanaka F,Saitsu H,Matsumoto N

Effect of TBCD and its regulatory interactor Arl2 on tubulin and microtubule integrity.
Cytoskeleton (Hoboken, N.J.)67(11)706-14(2010 Nov)
Tian G,Thomas S,Cowan NJ

Gene and alternative splicing annotation with AIR.
Genome research15(1)54-66(2005 Jan)
Florea L,Di Francesco V,Miller J,Turner R,Yao A,Harris M,Walenz B,Mobarry C,Merkulov GV,Charlab R,Dew I,Deng Z,Istrail S,Li P,Sutton G

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Tbcd cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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