DEFB112 cDNA ORF clone,

Gene
Clones
gRNAs

The following DEFB112 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DEFB112 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPu169468	XM_025926152.1 Latest version!	Puma concolor defensin beta 112 (DEFB112), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DEFB112 ( XM_025926152.1 ) cDNA ORF clone, -> XP_025781937.1 Puma concolor defensin beta 112 (DEFB112), mRNA.

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CloneID	OPu169468
Clone ID Related Accession (Same CDS sequence)	XM_025926152.1
Accession Version	XM_025926152.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 264bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-07-25
Organism	Puma concolor(puma)
Product	beta-defensin 112
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_020339445.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Puma concolor Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCATAT GTACTAAAAG GTTTGAAAAA CTATACTCAG AGATACATAA TTCTTCCACA 
ATATCTGAAA AGGCAGAATA CAGTACAAAG GAAAACAAAG CAGCTAGAAT TAAAGAGCAC 
TATGATGTTT TTGATCCATG GAAAGCCTGT ATAAAGCTTA ATGGTCAATG TAAAAATCAA 
TGTGGTGAAA ATGAATTTAA GATGTCATAC TGTGCAAGAC CTGAAACTCT TTGCTGCTTG 
AAAGAATGCG ACCCTGTCGA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025781937.1
CDS	1..264
Translation	MIICTKRFEKLYSEIHNSSTISEKAEYSTKENKAARIKEHYDVFDPWKACIKLNGQCKNQCGENEFKMSYCARPETLCCLKECDPVE

Target ORF information:

RefSeq Version	XM_025926152.1
Organism	Puma concolor(puma)
Definition	Puma concolor defensin beta 112 (DEFB112), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025926152.1

ORF Insert Sequence:

ATGATCATAT GTACTAAAAG GTTTGAAAAA CTATACTCAG AGATACATAA TTCTTCCACA 
ATATCTGAAA AGGCAGAATA CAGTACAAAG GAAAACAAAG CAGCTAGAAT TAAAGAGCAC 
TATGATGTTT TTGATCCATG GAAAGCCTGT ATAAAGCTTA ATGGTCAATG TAAAAATCAA 
TGTGGTGAAA ATGAATTTAA GATGTCATAC TGTGCAAGAC CTGAAACTCT TTGCTGCTTG 
AAAGAATGCG ACCCTGTCGA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.