| Gene Symbol | TLDC1 |
| Entrez Gene ID | 102877811 |
| Full Name | TBC/LysM-associated domain containing 1 |
| Gene Type | protein-coding |
| Organism | Pteropus alecto(black flying fox) |
ORF » Species Summary » Pteropus alecto » TLDC1 cDNA ORF clone
| Gene Symbol | TLDC1 |
| Entrez Gene ID | 102877811 |
| Full Name | TBC/LysM-associated domain containing 1 |
| Gene Type | protein-coding |
| Organism | Pteropus alecto(black flying fox) |
| mRNA | Protein | Name |
|---|---|---|
| XM_015600592.2 | XP_015456078.1 | TLD domain-containing protein 1 isoform X1 |
| XM_015600593.1 | XP_015456079.1 | TLD domain-containing protein 1 isoform X1 |
| XM_015600596.1 | XP_015456082.1 | TLD domain-containing protein 1 isoform X2 |
| XM_006926723.2 | XP_006926785.1 | TLD domain-containing protein 1 isoform X1 |
| XM_015600595.1 | XP_015456081.1 | TLD domain-containing protein 1 isoform X1 |
| XM_006926723.3 | XP_006926785.1 | TLD domain-containing protein 1 isoform X1 |
| XM_015600595.2 | XP_015456081.1 | TLD domain-containing protein 1 isoform X1 |
| XM_015600592.1 | XP_015456078.1 | TLD domain-containing protein 1 isoform X1 |
| XM_015600594.1 | XP_015456080.1 | TLD domain-containing protein 1 isoform X1 |
| XM_015600596.2 | XP_015456082.1 | TLD domain-containing protein 1 isoform X2 |
The following TLDC1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TLDC1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
| CloneID | OPt32199 | |
| Clone ID Related Accession (Same CDS sequence) | XM_015600592.2 , XM_015600593.1 , XM_006926723.2 , XM_015600595.1 , XM_006926723.3 , XM_015600595.2 , XM_015600592.1 , XM_015600594.1 | |
| Accession Version | XM_015600592.2 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 1365bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2018-05-06 | |
| Organism | Pteropus alecto(black flying fox) | |
| Product | TLD domain-containing protein 1 isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006494614.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015600592.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pteropus alecto Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGGGAAATA GCAAAAGCCG ATCAAGGCAG AATGTATGTT CACAGTTTCT TCCTGCAGAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_015456078.1 |
| CDS | 154..1518 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_015600592.2 |
1 | ATGGGAAATA GCAAAAGCCG ATCAAGGCAG AATGTATGTT CACAGTTTCT TCCTGCAGAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OPt32199 | |
| Clone ID Related Accession (Same CDS sequence) | XM_015600592.2 , XM_015600593.1 , XM_006926723.2 , XM_015600595.1 , XM_006926723.3 , XM_015600595.2 , XM_015600592.1 , XM_015600594.1 | |
| Accession Version | XM_015600593.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 1365bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2016-02-09 | |
| Organism | Pteropus alecto(black flying fox) | |
| Product | TLD domain-containing protein 1 isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006494614.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pteropus alecto Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGGGAAATA GCAAAAGCCG ATCAAGGCAG AATGTATGTT CACAGTTTCT TCCTGCAGAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_015456079.1 |
| CDS | 118..1482 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_015600593.1 |
1 | ATGGGAAATA GCAAAAGCCG ATCAAGGCAG AATGTATGTT CACAGTTTCT TCCTGCAGAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OPt32199 | |
| Clone ID Related Accession (Same CDS sequence) | XM_015600592.2 , XM_015600593.1 , XM_006926723.2 , XM_015600595.1 , XM_006926723.3 , XM_015600595.2 , XM_015600592.1 , XM_015600594.1 | |
| Accession Version | XM_006926723.2 | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 1365bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2016-02-09 | |
| Organism | Pteropus alecto(black flying fox) | |
| Product | TLD domain-containing protein 1 isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006494614.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Feb 10, 2016 this sequence version replaced XM_006926723.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pteropus alecto Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGGGAAATA GCAAAAGCCG ATCAAGGCAG AATGTATGTT CACAGTTTCT TCCTGCAGAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_006926785.1 |
| CDS | 146..1510 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_006926723.2 |
1 | ATGGGAAATA GCAAAAGCCG ATCAAGGCAG AATGTATGTT CACAGTTTCT TCCTGCAGAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OPt32199 | |
| Clone ID Related Accession (Same CDS sequence) | XM_015600592.2 , XM_015600593.1 , XM_006926723.2 , XM_015600595.1 , XM_006926723.3 , XM_015600595.2 , XM_015600592.1 , XM_015600594.1 | |
| Accession Version | XM_015600595.1 | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 1365bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2016-02-09 | |
| Organism | Pteropus alecto(black flying fox) | |
| Product | TLD domain-containing protein 1 isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006494614.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pteropus alecto Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGGGAAATA GCAAAAGCCG ATCAAGGCAG AATGTATGTT CACAGTTTCT TCCTGCAGAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_015456081.1 |
| CDS | 112..1476 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_015600595.1 |
1 | ATGGGAAATA GCAAAAGCCG ATCAAGGCAG AATGTATGTT CACAGTTTCT TCCTGCAGAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OPt32199 | |
| Clone ID Related Accession (Same CDS sequence) | XM_015600592.2 , XM_015600593.1 , XM_006926723.2 , XM_015600595.1 , XM_006926723.3 , XM_015600595.2 , XM_015600592.1 , XM_015600594.1 | |
| Accession Version | XM_006926723.3 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 1365bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2018-05-06 | |
| Organism | Pteropus alecto(black flying fox) | |
| Product | TLD domain-containing protein 1 isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006494614.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_006926723.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pteropus alecto Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGGGAAATA GCAAAAGCCG ATCAAGGCAG AATGTATGTT CACAGTTTCT TCCTGCAGAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_006926785.1 |
| CDS | 194..1558 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_006926723.3 |
1 | ATGGGAAATA GCAAAAGCCG ATCAAGGCAG AATGTATGTT CACAGTTTCT TCCTGCAGAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OPt32199 | |
| Clone ID Related Accession (Same CDS sequence) | XM_015600592.2 , XM_015600593.1 , XM_006926723.2 , XM_015600595.1 , XM_006926723.3 , XM_015600595.2 , XM_015600592.1 , XM_015600594.1 | |
| Accession Version | XM_015600595.2 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 1365bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2018-05-06 | |
| Organism | Pteropus alecto(black flying fox) | |
| Product | TLD domain-containing protein 1 isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006494614.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015600595.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pteropus alecto Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGGGAAATA GCAAAAGCCG ATCAAGGCAG AATGTATGTT CACAGTTTCT TCCTGCAGAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_015456081.1 |
| CDS | 115..1479 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_015600595.2 |
1 | ATGGGAAATA GCAAAAGCCG ATCAAGGCAG AATGTATGTT CACAGTTTCT TCCTGCAGAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OPt32199 | |
| Clone ID Related Accession (Same CDS sequence) | XM_015600592.2 , XM_015600593.1 , XM_006926723.2 , XM_015600595.1 , XM_006926723.3 , XM_015600595.2 , XM_015600592.1 , XM_015600594.1 | |
| Accession Version | XM_015600592.1 | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 1365bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2016-02-09 | |
| Organism | Pteropus alecto(black flying fox) | |
| Product | TLD domain-containing protein 1 isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006494614.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pteropus alecto Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGGGAAATA GCAAAAGCCG ATCAAGGCAG AATGTATGTT CACAGTTTCT TCCTGCAGAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_015456078.1 |
| CDS | 110..1474 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_015600592.1 |
1 | ATGGGAAATA GCAAAAGCCG ATCAAGGCAG AATGTATGTT CACAGTTTCT TCCTGCAGAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OPt32199 | |
| Clone ID Related Accession (Same CDS sequence) | XM_015600592.2 , XM_015600593.1 , XM_006926723.2 , XM_015600595.1 , XM_006926723.3 , XM_015600595.2 , XM_015600592.1 , XM_015600594.1 | |
| Accession Version | XM_015600594.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 1365bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2016-02-09 | |
| Organism | Pteropus alecto(black flying fox) | |
| Product | TLD domain-containing protein 1 isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006494614.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pteropus alecto Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGGGAAATA GCAAAAGCCG ATCAAGGCAG AATGTATGTT CACAGTTTCT TCCTGCAGAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_015456080.1 |
| CDS | 98..1462 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_015600594.1 |
1 | ATGGGAAATA GCAAAAGCCG ATCAAGGCAG AATGTATGTT CACAGTTTCT TCCTGCAGAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OPt32200 | |
| Clone ID Related Accession (Same CDS sequence) | XM_015600596.1 , XM_015600596.2 | |
| Accession Version | XM_015600596.1 | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 1245bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2016-02-09 | |
| Organism | Pteropus alecto(black flying fox) | |
| Product | TLD domain-containing protein 1 isoform X2 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006494614.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pteropus alecto Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGGGAAATA GCAAAAGCCG ATCAAGGCAG AATAGCCACA TTGGAGAAGC TCTTCCCCCA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_015456082.1 |
| CDS | 146..1390 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_015600596.1 |
1 | ATGGGAAATA GCAAAAGCCG ATCAAGGCAG AATAGCCACA TTGGAGAAGC TCTTCCCCCA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OPt32200 | |
| Clone ID Related Accession (Same CDS sequence) | XM_015600596.1 , XM_015600596.2 | |
| Accession Version | XM_015600596.2 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 1245bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2018-05-06 | |
| Organism | Pteropus alecto(black flying fox) | |
| Product | TLD domain-containing protein 1 isoform X2 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006494614.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015600596.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pteropus alecto Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGGGAAATA GCAAAAGCCG ATCAAGGCAG AATAGCCACA TTGGAGAAGC TCTTCCCCCA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_015456082.1 |
| CDS | 198..1442 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_015600596.2 |
1 | ATGGGAAATA GCAAAAGCCG ATCAAGGCAG AATAGCCACA TTGGAGAAGC TCTTCCCCCA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.