ROBO4 cDNA ORF clone, Propithecus coquereli(Coquerel's sifaka)

Gene
Clones
gRNAs

Summary

Gene Symbol	ROBO4
Entrez Gene ID	105823540
Full Name	roundabout guidance receptor 4
Gene Type	protein-coding
Organism	Propithecus coquereli(Coquerel's sifaka)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012660777.1	XP_012516231.1	roundabout homolog 4 isoform X1
XM_012660778.1	XP_012516232.1	roundabout homolog 4 isoform X2

Pathways from BioSystems
Gene Ontology

The following ROBO4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ROBO4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPr116998	XM_012660777.1 Latest version!	Propithecus coquereli roundabout, axon guidance receptor, homolog 4 (Drosophila) (ROBO4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OPr116999	XM_012660778.1 Latest version!	Propithecus coquereli roundabout, axon guidance receptor, homolog 4 (Drosophila) (ROBO4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ROBO4 ( XM_012660777.1 ) cDNA ORF clone, Propithecus coquereli(Coquerel's sifaka) -> XP_012516231.1 Propithecus coquereli roundabout, axon guidance receptor, homolog 4 (Drosophila) (ROBO4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OPr116998
Clone ID Related Accession (Same CDS sequence)	XM_012660777.1
Accession Version	XM_012660777.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3033bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-05-31
Organism	Propithecus coquereli(Coquerel's sifaka)
Product	roundabout homolog 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_012152818.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Propithecus coquereli Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCTCTG GAGGAGTGAG CCTCCCAGGG GCCGGGCGGC CCCTGCCCCT CCTGCTCCTG 
CTCCTCACGG GAGGCATGGC CCAGGACTCC CCGCCCCAGA TCCTAGTCCA CCCCCAGGAC 
CAGCTGCTCC AGGGCCCTGG CCCCGCCAGG ATGAGCTGCC GAGCCTCCGG CCAGCCGCCT 
CCCACCATCC GCTGGCTGCT GAATGGGCAG CCCTTGAGCA TGGTGCCCCC AGACCTACAC 
CACCTCCTAC CTGATGGAAC CCTCCTGCTG CTGCGGCCTC CTGCCCAGGG ACGGGCCCAC 
GATGACCAGG CCCAATCCGC AGACCTGGGC ATCTACACCT GTGAGGCCAG CAACCACCTG 
GGCACAGCAG TCAGCCGAGG TGCTCGGCTG TTTGTGGCTG TCCTCCGGGA GGATTTCCAG 
ATCCAACCTC GGGACACAGA GGCTGTGGAG GGCGAGCAGG TGATTCTGGA ATGTGGGCCG 
CCCTGGGGCC ACCCGGAGCC CACAGTGTCA TGGTGGAAAG ATGGGAAACC CCTGGCCCTC 
CAGCCGGGGC GGCACACGGT GTCCAGGGGT TCCCTGCTGA TGGCAAAAGC AGAGAAGAGT 
GACGCAGGGA CCTATATGTG TGTGGCCGCC AACAACGCAG GGCAGCGGGA GAGCCGAGCA 
GCCAGGGTGT CTGTCCAGGA GCCCCAGGAT TTCAAGGAGC CTTTGGAGCT TCTGGCTGTG 
CGCATTCGGC TGGAAAACGT GACACTGCTG CAGCCGGACC CCGCAAAGGG CCCCAAGCCT 
GGGCCTGCCG TGTGGCTCAG CTGGAAGGTG AGTGGCCCTG CTGCACCCGC TCAGTCCTAC 
ACGGCCTTGT TCAGGGCCCA GACTGCCCCA GGAGGCCAGG GAGCTCCGTG GGCAGAGGCG 
TTGCTCGACG GCTGGCAGAG CGCAGAGCTT GGGGGCCTCC ACTGGGGCCA AGACTATGAG 
TTCAAAGTGA GACCATCCTC CGGCCGGGCT CGGGGCCCCG ACAGCAACGT GCTGCTCCTG 
AGGCTGCCGG AACAAGTGCC CAGTGCCCCA CCCCAGGAGG TGACCCTAAA ACCTGGCAAT 
GGCAGTGTCC TTGTGAGCTG GGTTCCACCA CCTGCTGAAA ACCACAACGG CGTCATCCGT 
GGCTACCAGG TCTGGAGCCT GGGCAACACC TCACTGCCCC CAGCCAACTG GACTGTAGTG 
GGAGAGCAGA CCCAGCTGGA GATCGCCACC CGAATGCCAG GCTCCTACTG CGTGCAAGTG 
GCTGCAGTCA CTGGCGCTGG GGCTGGGGAG CCCAGTAGCC CTGTGTGCCT CCTTTTAGAG 
CAGGCCATGG AGCGAGCCGC CCGAGAACCC CGTGAGCATG GTCCCTGGAT CCTGGAGCAG 
CTGAGAGCCA CCTTGAGGCG GCCAGAGGTC ATTGCCAGCG GGGGTGTTGT TCTCTGGCTG 
CTGCTGCTGG GCACCGCTGT GTGTATCCAC CGCCGGCGCA GAGCTGGGGT GCACCTGGGC 
CCAGGTCTGT ACAGGTATAC CAGCGAGGAT GCCATCCTAA AACACAGGAT GGATCACAGC 
GACTCCCCCT GGCTGGCGGA TACTTGGCGT TCCACCTCGG GCTCCCGGGA CCTGAGCAGC 
AGCAGCAGCC TCAGCAGTCG GCTGGGAGTG GACCCCCGGG ACCCCCTAGA CAGTCGTCGC 
TCCTTGATCT CCTGGGACCC CCGAAGCCCC GGTGTGCCCC TGCTTCCAGA CACCAGCACT 
TTTTATGGCT CCCTCATCGC TGAGCTGCCC TCCAGCCCCC CAGCCCGGCC AAGCCCCCAG 
GCCCCAGCTG TCAGGCGCCT CCCGCCCCAG CTGGCCCGGC TCTCCAGCCC CTGGGCCAGC 
TCAGACAGCC TCTGCAGCCA CAGGGCGCTC TCTTCTCCAC GCTTGTCTCT GGCCCCCGCA 
GAGGCTTGGA AGGCCAAAAG GAAGCAGGAG CTGCACCAAG TCAACAGCTC CCCACTGCTC 
CGGGCCAGCC ACCCCCTGGA GCTCCAGGCC TGGGAGCTGG GAAACAGAGG CTCCAAGAAC 
CTTTCCCAAA GCCCAGGCCG CTGTTCCCCA GGAGCTGTGC CCCGAGCTCT GGTTGCCTGG 
TGGGCCCTGG GACCGCAGCT CCGTAGGGCC TCAAATGAGC TGGTTACTCG CCCTCTCCCT 
CCAGCACCCC TCTCTCCTCG TGGAAGCCCC ATGCAGAGTC AACAGACCCA GCACCTGGTG 
GCACCCCAAG CTCCATCCTC TGTCCCGCTG CCAGCAGCCC CTGTCCCCAT CCTTAGCCCC 
AGCGGCACCT CCAGCCCCCA GGCCTCTTCC CTCTCTGGCC CCAGCCCAGC TTCCAGTCGC 
CTGTCCAGCT CTTCACTGTC CTCCCTGGGG GAGGATCAGG ACAGTGTGCT GACCCCTGAA 
GAGGTGGCCC TGTGCCTGGA ACTCAGTGAG GGTGAGGAGA CCCCCAGGAA CAGTGTCTCT 
CCTGCGCCAC GGGCTCCTTC ACCCCCTACC ACGTATGGGT ACATCAGCGT CCCAACAGCC 
TCAGAGCTAG CAGACATGGG CAGGACAGGC GGAGGGGTAG GCTCTGGAGC AGGGAGCCTG 
TTCCCACCTC GGCCCTGCCC CACCCCGACC CCCAGTGAGG GCTCCTTGGC CAATGGTTGG 
GGCTCAGCCT CAGAGGACAA TGCCCCCAGC GCCAGAGCCA GCCTCGTCAG CTCCTCCGAT 
GGCTCCTTCC TTGCGGATGC CCACTTTGCC CATGCCCTGG CCGTGGCTGT GGATAGCTTC 
AGTTTCGGTC TGGAGCCCAG GGAGGCAGAC TGCGTCTTCA TGGATGCCTC ATCACCTCCC 
TCCCCGAGGG ATGACCTCTT CCTCACCCCC ACCCTCTCCC TGCCCCTGTG GGAGTGGAGG 
CCAGACTGGT TGGAGGACGT GGAGACCAAC CACACCCAGC GGCTGGGAAG GGGGCTGTCT 
CCCTGGCCCC CTGACTCTCG GATCTCTTCC CAGAGCAGTC GGCTCAGCTG TCCTGTGCCC 
AAGGCTGGTG CTTCTCCCAT AGATTCCTCA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012516231.1
CDS	133..3165
Translation	MGSGGVSLPGAGRPLPLLLLLLTGGMAQDSPPQILVHPQDQLLQGPGPARMSCRASGQPPPTIRWLLNGQPLSMVPPDLHHLLPDGTLLLLRPPAQGRAHDDQAQSADLGIYTCEASNHLGTAVSRGARLFVAVLREDFQIQPRDTEAVEGEQVILECGPPWGHPEPTVSWWKDGKPLALQPGRHTVSRGSLLMAKAEKSDAGTYMCVAANNAGQRESRAARVSVQEPQDFKEPLELLAVRIRLENVTLLQPDPAKGPKPGPAVWLSWKVSGPAAPAQSYTALFRAQTAPGGQGAPWAEALLDGWQSAELGGLHWGQDYEFKVRPSSGRARGPDSNVLLLRLPEQVPSAPPQEVTLKPGNGSVLVSWVPPPAENHNGVIRGYQVWSLGNTSLPPANWTVVGEQTQLEIATRMPGSYCVQVAAVTGAGAGEPSSPVCLLLEQAMERAAREPREHGPWILEQLRATLRRPEVIASGGVVLWLLLLGTAVCIHRRRRAGVHLGPGLYRYTSEDAILKHRMDHSDSPWLADTWRSTSGSRDLSSSSSLSSRLGVDPRDPLDSRRSLISWDPRSPGVPLLPDTSTFYGSLIAELPSSPPARPSPQAPAVRRLPPQLARLSSPWASSDSLCSHRALSSPRLSLAPAEAWKAKRKQELHQVNSSPLLRASHPLELQAWELGNRGSKNLSQSPGRCSPGAVPRALVAWWALGPQLRRASNELVTRPLPPAPLSPRGSPMQSQQTQHLVAPQAPSSVPLPAAPVPILSPSGTSSPQASSLSGPSPASSRLSSSSLSSLGEDQDSVLTPEEVALCLELSEGEETPRNSVSPAPRAPSPPTTYGYISVPTASELADMGRTGGGVGSGAGSLFPPRPCPTPTPSEGSLANGWGSASEDNAPSARASLVSSSDGSFLADAHFAHALAVAVDSFSFGLEPREADCVFMDASSPPSPRDDLFLTPTLSLPLWEWRPDWLEDVETNHTQRLGRGLSPWPPDSRISSQSSRLSCPVPKAGASPIDSS

Target ORF information:

RefSeq Version	XM_012660777.1
Organism	Propithecus coquereli(Coquerel's sifaka)
Definition	Propithecus coquereli roundabout, axon guidance receptor, homolog 4 (Drosophila) (ROBO4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012660777.1

ORF Insert Sequence:

ATGGGCTCTG GAGGAGTGAG CCTCCCAGGG GCCGGGCGGC CCCTGCCCCT CCTGCTCCTG 
CTCCTCACGG GAGGCATGGC CCAGGACTCC CCGCCCCAGA TCCTAGTCCA CCCCCAGGAC 
CAGCTGCTCC AGGGCCCTGG CCCCGCCAGG ATGAGCTGCC GAGCCTCCGG CCAGCCGCCT 
CCCACCATCC GCTGGCTGCT GAATGGGCAG CCCTTGAGCA TGGTGCCCCC AGACCTACAC 
CACCTCCTAC CTGATGGAAC CCTCCTGCTG CTGCGGCCTC CTGCCCAGGG ACGGGCCCAC 
GATGACCAGG CCCAATCCGC AGACCTGGGC ATCTACACCT GTGAGGCCAG CAACCACCTG 
GGCACAGCAG TCAGCCGAGG TGCTCGGCTG TTTGTGGCTG TCCTCCGGGA GGATTTCCAG 
ATCCAACCTC GGGACACAGA GGCTGTGGAG GGCGAGCAGG TGATTCTGGA ATGTGGGCCG 
CCCTGGGGCC ACCCGGAGCC CACAGTGTCA TGGTGGAAAG ATGGGAAACC CCTGGCCCTC 
CAGCCGGGGC GGCACACGGT GTCCAGGGGT TCCCTGCTGA TGGCAAAAGC AGAGAAGAGT 
GACGCAGGGA CCTATATGTG TGTGGCCGCC AACAACGCAG GGCAGCGGGA GAGCCGAGCA 
GCCAGGGTGT CTGTCCAGGA GCCCCAGGAT TTCAAGGAGC CTTTGGAGCT TCTGGCTGTG 
CGCATTCGGC TGGAAAACGT GACACTGCTG CAGCCGGACC CCGCAAAGGG CCCCAAGCCT 
GGGCCTGCCG TGTGGCTCAG CTGGAAGGTG AGTGGCCCTG CTGCACCCGC TCAGTCCTAC 
ACGGCCTTGT TCAGGGCCCA GACTGCCCCA GGAGGCCAGG GAGCTCCGTG GGCAGAGGCG 
TTGCTCGACG GCTGGCAGAG CGCAGAGCTT GGGGGCCTCC ACTGGGGCCA AGACTATGAG 
TTCAAAGTGA GACCATCCTC CGGCCGGGCT CGGGGCCCCG ACAGCAACGT GCTGCTCCTG 
AGGCTGCCGG AACAAGTGCC CAGTGCCCCA CCCCAGGAGG TGACCCTAAA ACCTGGCAAT 
GGCAGTGTCC TTGTGAGCTG GGTTCCACCA CCTGCTGAAA ACCACAACGG CGTCATCCGT 
GGCTACCAGG TCTGGAGCCT GGGCAACACC TCACTGCCCC CAGCCAACTG GACTGTAGTG 
GGAGAGCAGA CCCAGCTGGA GATCGCCACC CGAATGCCAG GCTCCTACTG CGTGCAAGTG 
GCTGCAGTCA CTGGCGCTGG GGCTGGGGAG CCCAGTAGCC CTGTGTGCCT CCTTTTAGAG 
CAGGCCATGG AGCGAGCCGC CCGAGAACCC CGTGAGCATG GTCCCTGGAT CCTGGAGCAG 
CTGAGAGCCA CCTTGAGGCG GCCAGAGGTC ATTGCCAGCG GGGGTGTTGT TCTCTGGCTG 
CTGCTGCTGG GCACCGCTGT GTGTATCCAC CGCCGGCGCA GAGCTGGGGT GCACCTGGGC 
CCAGGTCTGT ACAGGTATAC CAGCGAGGAT GCCATCCTAA AACACAGGAT GGATCACAGC 
GACTCCCCCT GGCTGGCGGA TACTTGGCGT TCCACCTCGG GCTCCCGGGA CCTGAGCAGC 
AGCAGCAGCC TCAGCAGTCG GCTGGGAGTG GACCCCCGGG ACCCCCTAGA CAGTCGTCGC 
TCCTTGATCT CCTGGGACCC CCGAAGCCCC GGTGTGCCCC TGCTTCCAGA CACCAGCACT 
TTTTATGGCT CCCTCATCGC TGAGCTGCCC TCCAGCCCCC CAGCCCGGCC AAGCCCCCAG 
GCCCCAGCTG TCAGGCGCCT CCCGCCCCAG CTGGCCCGGC TCTCCAGCCC CTGGGCCAGC 
TCAGACAGCC TCTGCAGCCA CAGGGCGCTC TCTTCTCCAC GCTTGTCTCT GGCCCCCGCA 
GAGGCTTGGA AGGCCAAAAG GAAGCAGGAG CTGCACCAAG TCAACAGCTC CCCACTGCTC 
CGGGCCAGCC ACCCCCTGGA GCTCCAGGCC TGGGAGCTGG GAAACAGAGG CTCCAAGAAC 
CTTTCCCAAA GCCCAGGCCG CTGTTCCCCA GGAGCTGTGC CCCGAGCTCT GGTTGCCTGG 
TGGGCCCTGG GACCGCAGCT CCGTAGGGCC TCAAATGAGC TGGTTACTCG CCCTCTCCCT 
CCAGCACCCC TCTCTCCTCG TGGAAGCCCC ATGCAGAGTC AACAGACCCA GCACCTGGTG 
GCACCCCAAG CTCCATCCTC TGTCCCGCTG CCAGCAGCCC CTGTCCCCAT CCTTAGCCCC 
AGCGGCACCT CCAGCCCCCA GGCCTCTTCC CTCTCTGGCC CCAGCCCAGC TTCCAGTCGC 
CTGTCCAGCT CTTCACTGTC CTCCCTGGGG GAGGATCAGG ACAGTGTGCT GACCCCTGAA 
GAGGTGGCCC TGTGCCTGGA ACTCAGTGAG GGTGAGGAGA CCCCCAGGAA CAGTGTCTCT 
CCTGCGCCAC GGGCTCCTTC ACCCCCTACC ACGTATGGGT ACATCAGCGT CCCAACAGCC 
TCAGAGCTAG CAGACATGGG CAGGACAGGC GGAGGGGTAG GCTCTGGAGC AGGGAGCCTG 
TTCCCACCTC GGCCCTGCCC CACCCCGACC CCCAGTGAGG GCTCCTTGGC CAATGGTTGG 
GGCTCAGCCT CAGAGGACAA TGCCCCCAGC GCCAGAGCCA GCCTCGTCAG CTCCTCCGAT 
GGCTCCTTCC TTGCGGATGC CCACTTTGCC CATGCCCTGG CCGTGGCTGT GGATAGCTTC 
AGTTTCGGTC TGGAGCCCAG GGAGGCAGAC TGCGTCTTCA TGGATGCCTC ATCACCTCCC 
TCCCCGAGGG ATGACCTCTT CCTCACCCCC ACCCTCTCCC TGCCCCTGTG GGAGTGGAGG 
CCAGACTGGT TGGAGGACGT GGAGACCAAC CACACCCAGC GGCTGGGAAG GGGGCTGTCT 
CCCTGGCCCC CTGACTCTCG GATCTCTTCC CAGAGCAGTC GGCTCAGCTG TCCTGTGCCC 
AAGGCTGGTG CTTCTCCCAT AGATTCCTCA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ROBO4 ( XM_012660778.1 ) cDNA ORF clone, Propithecus coquereli(Coquerel's sifaka) -> XP_012516232.1 Propithecus coquereli roundabout, axon guidance receptor, homolog 4 (Drosophila) (ROBO4), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OPr116999
Clone ID Related Accession (Same CDS sequence)	XM_012660778.1
Accession Version	XM_012660778.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3018bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-05-31
Organism	Propithecus coquereli(Coquerel's sifaka)
Product	roundabout homolog 4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_012152818.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Propithecus coquereli Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCTCTG GAGGAGTGAG CCTCCCAGGG GCCGGGCGGC CCCTGCCCCT CCTGCTCCTG 
CTCCTCACGG GAGGCATGGC CCAGGACTCC CCGCCCCAGA TCCTAGTCCA CCCCCAGGAC 
CAGCTGCTCC AGGGCCCTGG CCCCGCCAGG ATGAGCTGCC GAGCCTCCGG CCAGCCGCCT 
CCCACCATCC GCTGGCTGCT GAATGGGCAG CCCTTGAGCA TGGTGCCCCC AGACCTACAC 
CACCTCCTAC CTGATGGAAC CCTCCTGCTG CTGCGGCCTC CTGCCCAGGG ACGGGCCCAC 
GATGACCAGG CCCAATCCGC AGACCTGGGC ATCTACACCT GTGAGGCCAG CAACCACCTG 
GGCACAGCAG TCAGCCGAGG TGCTCGGCTG TTTGTGGCTG TCCTCCGGGA GGATTTCCAG 
ATCCAACCTC GGGACACAGA GGCTGTGGAG GGCGAGCAGG TGATTCTGGA ATGTGGGCCG 
CCCTGGGGCC ACCCGGAGCC CACAGTGTCA TGGTGGAAAG ATGGGAAACC CCTGGCCCTC 
CAGCCGGGGC GGCACACGGT GTCCAGGGGT TCCCTGCTGA TGGCAAAAGC AGAGAAGAGT 
GACGCAGGGA CCTATATGTG TGTGGCCGCC AACAACGCAG GGCAGCGGGA GAGCCGAGCA 
GCCAGGGTGT CTGTCCAGGA GCCCCAGGAT TTCAAGGAGC CTTTGGAGCT TCTGGCTGTG 
CGCATTCGGC TGGAAAACGT GACACTGCTG CAGCCGGACC CCGCAAAGGG CCCCAAGCCT 
GGGCCTGCCG TGTGGCTCAG CTGGAAGGTG AGTGGCCCTG CTGCACCCGC TCAGTCCTAC 
ACGGCCTTGT TCAGGGCCCA GACTGCCCCA GGAGGCCAGG GAGCTCCGTG GGCAGAGGCG 
TTGCTCGACG GCTGGCAGAG CGCAGAGCTT GGGGGCCTCC ACTGGGGCCA AGACTATGAG 
TTCAAAGTGA GACCATCCTC CGGCCGGGCT CGGGGCCCCG ACAGCAACGT GCTGCTCCTG 
AGGCTGCCGG AACAAGTGCC CAGTGCCCCA CCCCAGGAGG TGACCCTAAA ACCTGGCAAT 
GGCAGTGTCC TTGTGAGCTG GGTTCCACCA CCTGCTGAAA ACCACAACGG CGTCATCCGT 
GGCTACCAGG TCTGGAGCCT GGGCAACACC TCACTGCCCC CAGCCAACTG GACTGTAGTG 
GGAGAGCAGA CCCAGCTGGA GATCGCCACC CGAATGCCAG GCTCCTACTG CGTGCAAGTG 
GCTGCAGTCA CTGGCGCTGG GGCTGGGGAG CCCAGTAGCC CTGTGTGCCT CCTTTTAGAG 
CAGGCCATGG AGCGAGCCGC CCGAGAACCC CGTGAGCATG GTCCCTGGAT CCTGGAGCAG 
CTGAGAGCCA CCTTGAGGCG GCCAGAGGTC ATTGCCAGCG GGGGTGTTGT TCTCTGGCTG 
CTGCTGCTGG GCACCGCTGT GTGTATCCAC CGCCGGCGCA GAGCTGGGGT GCACCTGGGC 
CCAGGTCTGT ACAGGTATAC CAGCGAGGAT GCCATCCTAA AACACAGGAT GGATCACAGC 
GACTCCCCCT GGCTGGCGGA TACTTGGCGT TCCACCTCGG GCTCCCGGGA CCTGAGCAGC 
AGCAGCAGCC TCAGCAGTCG GCTGGGAGTG GACCCCCGGG ACCCCCTAGA CAGTCGTCGC 
TCCTTGATCT CCTGGGACCC CCGAAGCCCC GGTGTGCCCC TGCTTCCAGA CACCAGCACT 
TTTTATGGCT CCCTCATCGC TGAGCTGCCC TCCAGCCCCC CAGCCCGGCC AAGCCCCCAG 
GCCCCAGCTG TCAGGCGCCT CCCGCCCCAG CTGGCCCGGC TCTCCAGCCC CTGGGCCAGC 
TCAGACAGCC TCTGCAGCCA CAGGGCGCTC TCTTCTCCAC GCTTGTCTCT GGCCCCCGCA 
GAGGCTTGGA AGGCCAAAAG GAAGCAGGAG CTGCACCAAG TCAACAGCTC CCCACTGCTC 
CGGGCCAGCC ACCCCCTGGA GCTCCAGGCC TGGGAGCTGG GAAACAGAGG CTCCAAGAAC 
CTTTCCCAAA GCCCAGGAGC TGTGCCCCGA GCTCTGGTTG CCTGGTGGGC CCTGGGACCG 
CAGCTCCGTA GGGCCTCAAA TGAGCTGGTT ACTCGCCCTC TCCCTCCAGC ACCCCTCTCT 
CCTCGTGGAA GCCCCATGCA GAGTCAACAG ACCCAGCACC TGGTGGCACC CCAAGCTCCA 
TCCTCTGTCC CGCTGCCAGC AGCCCCTGTC CCCATCCTTA GCCCCAGCGG CACCTCCAGC 
CCCCAGGCCT CTTCCCTCTC TGGCCCCAGC CCAGCTTCCA GTCGCCTGTC CAGCTCTTCA 
CTGTCCTCCC TGGGGGAGGA TCAGGACAGT GTGCTGACCC CTGAAGAGGT GGCCCTGTGC 
CTGGAACTCA GTGAGGGTGA GGAGACCCCC AGGAACAGTG TCTCTCCTGC GCCACGGGCT 
CCTTCACCCC CTACCACGTA TGGGTACATC AGCGTCCCAA CAGCCTCAGA GCTAGCAGAC 
ATGGGCAGGA CAGGCGGAGG GGTAGGCTCT GGAGCAGGGA GCCTGTTCCC ACCTCGGCCC 
TGCCCCACCC CGACCCCCAG TGAGGGCTCC TTGGCCAATG GTTGGGGCTC AGCCTCAGAG 
GACAATGCCC CCAGCGCCAG AGCCAGCCTC GTCAGCTCCT CCGATGGCTC CTTCCTTGCG 
GATGCCCACT TTGCCCATGC CCTGGCCGTG GCTGTGGATA GCTTCAGTTT CGGTCTGGAG 
CCCAGGGAGG CAGACTGCGT CTTCATGGAT GCCTCATCAC CTCCCTCCCC GAGGGATGAC 
CTCTTCCTCA CCCCCACCCT CTCCCTGCCC CTGTGGGAGT GGAGGCCAGA CTGGTTGGAG 
GACGTGGAGA CCAACCACAC CCAGCGGCTG GGAAGGGGGC TGTCTCCCTG GCCCCCTGAC 
TCTCGGATCT CTTCCCAGAG CAGTCGGCTC AGCTGTCCTG TGCCCAAGGC TGGTGCTTCT 
CCCATAGATT CCTCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012516232.1
CDS	133..3150
Translation	MGSGGVSLPGAGRPLPLLLLLLTGGMAQDSPPQILVHPQDQLLQGPGPARMSCRASGQPPPTIRWLLNGQPLSMVPPDLHHLLPDGTLLLLRPPAQGRAHDDQAQSADLGIYTCEASNHLGTAVSRGARLFVAVLREDFQIQPRDTEAVEGEQVILECGPPWGHPEPTVSWWKDGKPLALQPGRHTVSRGSLLMAKAEKSDAGTYMCVAANNAGQRESRAARVSVQEPQDFKEPLELLAVRIRLENVTLLQPDPAKGPKPGPAVWLSWKVSGPAAPAQSYTALFRAQTAPGGQGAPWAEALLDGWQSAELGGLHWGQDYEFKVRPSSGRARGPDSNVLLLRLPEQVPSAPPQEVTLKPGNGSVLVSWVPPPAENHNGVIRGYQVWSLGNTSLPPANWTVVGEQTQLEIATRMPGSYCVQVAAVTGAGAGEPSSPVCLLLEQAMERAAREPREHGPWILEQLRATLRRPEVIASGGVVLWLLLLGTAVCIHRRRRAGVHLGPGLYRYTSEDAILKHRMDHSDSPWLADTWRSTSGSRDLSSSSSLSSRLGVDPRDPLDSRRSLISWDPRSPGVPLLPDTSTFYGSLIAELPSSPPARPSPQAPAVRRLPPQLARLSSPWASSDSLCSHRALSSPRLSLAPAEAWKAKRKQELHQVNSSPLLRASHPLELQAWELGNRGSKNLSQSPGAVPRALVAWWALGPQLRRASNELVTRPLPPAPLSPRGSPMQSQQTQHLVAPQAPSSVPLPAAPVPILSPSGTSSPQASSLSGPSPASSRLSSSSLSSLGEDQDSVLTPEEVALCLELSEGEETPRNSVSPAPRAPSPPTTYGYISVPTASELADMGRTGGGVGSGAGSLFPPRPCPTPTPSEGSLANGWGSASEDNAPSARASLVSSSDGSFLADAHFAHALAVAVDSFSFGLEPREADCVFMDASSPPSPRDDLFLTPTLSLPLWEWRPDWLEDVETNHTQRLGRGLSPWPPDSRISSQSSRLSCPVPKAGASPIDSS

Target ORF information:

RefSeq Version	XM_012660778.1
Organism	Propithecus coquereli(Coquerel's sifaka)
Definition	Propithecus coquereli roundabout, axon guidance receptor, homolog 4 (Drosophila) (ROBO4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012660778.1

ORF Insert Sequence:

ATGGGCTCTG GAGGAGTGAG CCTCCCAGGG GCCGGGCGGC CCCTGCCCCT CCTGCTCCTG 
CTCCTCACGG GAGGCATGGC CCAGGACTCC CCGCCCCAGA TCCTAGTCCA CCCCCAGGAC 
CAGCTGCTCC AGGGCCCTGG CCCCGCCAGG ATGAGCTGCC GAGCCTCCGG CCAGCCGCCT 
CCCACCATCC GCTGGCTGCT GAATGGGCAG CCCTTGAGCA TGGTGCCCCC AGACCTACAC 
CACCTCCTAC CTGATGGAAC CCTCCTGCTG CTGCGGCCTC CTGCCCAGGG ACGGGCCCAC 
GATGACCAGG CCCAATCCGC AGACCTGGGC ATCTACACCT GTGAGGCCAG CAACCACCTG 
GGCACAGCAG TCAGCCGAGG TGCTCGGCTG TTTGTGGCTG TCCTCCGGGA GGATTTCCAG 
ATCCAACCTC GGGACACAGA GGCTGTGGAG GGCGAGCAGG TGATTCTGGA ATGTGGGCCG 
CCCTGGGGCC ACCCGGAGCC CACAGTGTCA TGGTGGAAAG ATGGGAAACC CCTGGCCCTC 
CAGCCGGGGC GGCACACGGT GTCCAGGGGT TCCCTGCTGA TGGCAAAAGC AGAGAAGAGT 
GACGCAGGGA CCTATATGTG TGTGGCCGCC AACAACGCAG GGCAGCGGGA GAGCCGAGCA 
GCCAGGGTGT CTGTCCAGGA GCCCCAGGAT TTCAAGGAGC CTTTGGAGCT TCTGGCTGTG 
CGCATTCGGC TGGAAAACGT GACACTGCTG CAGCCGGACC CCGCAAAGGG CCCCAAGCCT 
GGGCCTGCCG TGTGGCTCAG CTGGAAGGTG AGTGGCCCTG CTGCACCCGC TCAGTCCTAC 
ACGGCCTTGT TCAGGGCCCA GACTGCCCCA GGAGGCCAGG GAGCTCCGTG GGCAGAGGCG 
TTGCTCGACG GCTGGCAGAG CGCAGAGCTT GGGGGCCTCC ACTGGGGCCA AGACTATGAG 
TTCAAAGTGA GACCATCCTC CGGCCGGGCT CGGGGCCCCG ACAGCAACGT GCTGCTCCTG 
AGGCTGCCGG AACAAGTGCC CAGTGCCCCA CCCCAGGAGG TGACCCTAAA ACCTGGCAAT 
GGCAGTGTCC TTGTGAGCTG GGTTCCACCA CCTGCTGAAA ACCACAACGG CGTCATCCGT 
GGCTACCAGG TCTGGAGCCT GGGCAACACC TCACTGCCCC CAGCCAACTG GACTGTAGTG 
GGAGAGCAGA CCCAGCTGGA GATCGCCACC CGAATGCCAG GCTCCTACTG CGTGCAAGTG 
GCTGCAGTCA CTGGCGCTGG GGCTGGGGAG CCCAGTAGCC CTGTGTGCCT CCTTTTAGAG 
CAGGCCATGG AGCGAGCCGC CCGAGAACCC CGTGAGCATG GTCCCTGGAT CCTGGAGCAG 
CTGAGAGCCA CCTTGAGGCG GCCAGAGGTC ATTGCCAGCG GGGGTGTTGT TCTCTGGCTG 
CTGCTGCTGG GCACCGCTGT GTGTATCCAC CGCCGGCGCA GAGCTGGGGT GCACCTGGGC 
CCAGGTCTGT ACAGGTATAC CAGCGAGGAT GCCATCCTAA AACACAGGAT GGATCACAGC 
GACTCCCCCT GGCTGGCGGA TACTTGGCGT TCCACCTCGG GCTCCCGGGA CCTGAGCAGC 
AGCAGCAGCC TCAGCAGTCG GCTGGGAGTG GACCCCCGGG ACCCCCTAGA CAGTCGTCGC 
TCCTTGATCT CCTGGGACCC CCGAAGCCCC GGTGTGCCCC TGCTTCCAGA CACCAGCACT 
TTTTATGGCT CCCTCATCGC TGAGCTGCCC TCCAGCCCCC CAGCCCGGCC AAGCCCCCAG 
GCCCCAGCTG TCAGGCGCCT CCCGCCCCAG CTGGCCCGGC TCTCCAGCCC CTGGGCCAGC 
TCAGACAGCC TCTGCAGCCA CAGGGCGCTC TCTTCTCCAC GCTTGTCTCT GGCCCCCGCA 
GAGGCTTGGA AGGCCAAAAG GAAGCAGGAG CTGCACCAAG TCAACAGCTC CCCACTGCTC 
CGGGCCAGCC ACCCCCTGGA GCTCCAGGCC TGGGAGCTGG GAAACAGAGG CTCCAAGAAC 
CTTTCCCAAA GCCCAGGAGC TGTGCCCCGA GCTCTGGTTG CCTGGTGGGC CCTGGGACCG 
CAGCTCCGTA GGGCCTCAAA TGAGCTGGTT ACTCGCCCTC TCCCTCCAGC ACCCCTCTCT 
CCTCGTGGAA GCCCCATGCA GAGTCAACAG ACCCAGCACC TGGTGGCACC CCAAGCTCCA 
TCCTCTGTCC CGCTGCCAGC AGCCCCTGTC CCCATCCTTA GCCCCAGCGG CACCTCCAGC 
CCCCAGGCCT CTTCCCTCTC TGGCCCCAGC CCAGCTTCCA GTCGCCTGTC CAGCTCTTCA 
CTGTCCTCCC TGGGGGAGGA TCAGGACAGT GTGCTGACCC CTGAAGAGGT GGCCCTGTGC 
CTGGAACTCA GTGAGGGTGA GGAGACCCCC AGGAACAGTG TCTCTCCTGC GCCACGGGCT 
CCTTCACCCC CTACCACGTA TGGGTACATC AGCGTCCCAA CAGCCTCAGA GCTAGCAGAC 
ATGGGCAGGA CAGGCGGAGG GGTAGGCTCT GGAGCAGGGA GCCTGTTCCC ACCTCGGCCC 
TGCCCCACCC CGACCCCCAG TGAGGGCTCC TTGGCCAATG GTTGGGGCTC AGCCTCAGAG 
GACAATGCCC CCAGCGCCAG AGCCAGCCTC GTCAGCTCCT CCGATGGCTC CTTCCTTGCG 
GATGCCCACT TTGCCCATGC CCTGGCCGTG GCTGTGGATA GCTTCAGTTT CGGTCTGGAG 
CCCAGGGAGG CAGACTGCGT CTTCATGGAT GCCTCATCAC CTCCCTCCCC GAGGGATGAC 
CTCTTCCTCA CCCCCACCCT CTCCCTGCCC CTGTGGGAGT GGAGGCCAGA CTGGTTGGAG 
GACGTGGAGA CCAACCACAC CCAGCGGCTG GGAAGGGGGC TGTCTCCCTG GCCCCCTGAC 
TCTCGGATCT CTTCCCAGAG CAGTCGGCTC AGCTGTCCTG TGCCCAAGGC TGGTGCTTCT 
CCCATAGATT CCTCATGA