TOP3A cDNA ORF clone, Pogona vitticeps(central bearded dragon)

Gene
Clones
gRNAs

Summary

Gene Symbol	TOP3A
Entrez Gene ID	110090801
Full Name	DNA topoisomerase III alpha
Gene Type	protein-coding
Organism	Pogona vitticeps(central bearded dragon)

mRNA and Protein(s)

mRNA	Protein	Name
XM_020814630.1	XP_020670289.1	DNA topoisomerase 3-alpha

Pathways from BioSystems
Gene Ontology

The following TOP3A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TOP3A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPo257870	XM_020814630.1 Latest version!	Pogona vitticeps topoisomerase (DNA) III alpha (TOP3A), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TOP3A ( XM_020814630.1 ) cDNA ORF clone, Pogona vitticeps(central bearded dragon) -> XP_020670289.1 Pogona vitticeps topoisomerase (DNA) III alpha (TOP3A), mRNA.

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CloneID	OPo257870
Clone ID Related Accession (Same CDS sequence)	XM_020814630.1
Accession Version	XM_020814630.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3054bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-04-09
Organism	Pogona vitticeps(central bearded dragon)
Product	DNA topoisomerase 3-alpha
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018151600.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pogona vitticeps Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCAGGA TGATCAGTCA GACTAGACTG TTTGCGCTCA GTGGAGTCCG ATTGTTCTTC 
TGTCCCCAGA AACTTTGCTT TTCTCAGGCT GCGGAAGGCA TGACGTTCCG AAACATCCAG 
AGAGTCCTCT GCGTGGCAGA GAAAAATGAT GCTGCCAAAG GAATTGCAGA CATCCTTTCT 
AACAGCAGGA TGAGGAGGAG GGAAGGATTT TCCAAGTATA ACAAGATTTA TGAATGCCAG 
TACCACCTCT TTGGCCAGAA CGTCACGGTG GTGATGACAT CCGTCTCGGG ACACTTGCTC 
TCCCATGACT TCAAGCTACC CTTTCGAAAG TGGCACAGCT GTAACCCACT TGTCCTTTTT 
GATGCTGACA TTGAGAAATT CTGCCCTGAA AACTACATGG ACATTAAGCG AACCCTTGAG 
CGAGAAGCTC AGCAGTGCCA AGCTCTGGTC ATCTGGACAG ACGGCGACCG CGAGGGAGAG 
AACATTGGCT TCGAAATCAT TCAAGTCTGC AAAGCAGTGA AGCCAAGCCT CCAGGTGTTC 
CGCGCCCGCT TCTCTGAGAT CACACCGAAC GCCATAAGAG CGGCCTGCCA GAACCTCACC 
CAGCCAGATC AGAACGTCAG TGATGCCGTC GATGTTAGGC AGGAACTGGA TCTCAGGATT 
GGTGCTGCGT TCACTCGGTT CCAGACTTTG AGGCTCCAGA AAATCTTCCC TGGCGTTTTA 
GCAGACCAGC TGATCAGCTA CGGGAGCTGT CAGTTCCCGA CGCTGGGCTT TGTGGTGGAA 
AGGTTCAAAG CCATCGAAGC CTTCGTTTCC GAGACCTTCT ACAAAATTAA AGTGACTCAC 
GAGCATGAAG AGGGGACGGT GGATTTCGGC TGGAAGAGGA ACCGTCTCTT CAACCATACA 
GCATGCCTCG TCCTTTACCA CATGTGCATG GAGGACCCAG TGGCCACAGT GGTGGAAGTT 
GGAAGCAGAC CAAAGAGCAA ATGGAGACCG CTGCCTCTGG ACACTGTGGA ACTCGAGAAG 
CTGGCTTCCC GTAAACTGAG GATAAATGCT AAAGAAACCA TGAAGATTGC CGAGAAGCTC 
TACACTCAAG GATACATAAG TTACCCCCGA ACAGAAACCA ACATTTTCCC CAGAGACCTC 
GACCTTTCGA ACCTGGTTCA GCTCCAGACA CAAGACCCTC ACTGGGGAGC CTTCGCACAG 
AGGATTCTGG ACCAGGGTGG GCCAACCCCA CGTCAGGGAA ATAAATCGGA TCAGGCCCAT 
CCTCCCATTC ATCCTACAAA ATACGGAGTG AATCTGCAGG GGAACGACCA GCGGCTGTAC 
GAATTCATTG TGCGCCATTT CCTGGCCTGC TGTTCCCGAG ATGCAAAAGG CCAGGAGACG 
ACAGTGGAGA TCGACATAGC CAAAGAGCGC TTCGTCGCTC ACGGGTTGAT GATCCTGGAG 
AGAAATTACT TGGAGGTCTA TCCGTACGAG AAGTGGAGCG ATAAGGTCAT CCCCGTTTAT 
GAGAAGGGGT CTCGCTTTCA GCCCACCACC GTGGAGATGG TCGACGGGGA CACCAGCCCG 
CCGCAGCTGC TGACGGAAGC TGATCTCATT TCCCTCATGG AGAAACACGG CATCGGTACG 
GACGCCACCC ACGCGGAGCA CATCGAAACC ATCAAATCCC GCATGTACGT GGGGCTCACG 
CCGGACCAGC GGTTCCTTCC AGGACATCTA GGGATGGGGC TGGTAGAAGG CTACGATTCC 
ATGGGCTACG AGATGTCCAA GCCTGACCTC CGAGCTGAGC TCGAGGCCGA CCTGAAGCTG 
ATCTGTGAGG GCAAGAAGGA CAAATCCGTG GTGCTGAGGG AGCAAATCCA GAAGTACAAG 
CAGGTCTTTG TAGAAGCGGT GCAGAAGGCC AAGAAGTTGG ATGAAGCGCT GTCTCGGTAT 
TTTGGAGACG TGACTCCTGT TTCAGAACAG GAAGACATTT ACCCCGCCAT GCCCGATCCC 
ATCAGGAAAT GTCCGCAGTG CAACAGCGAC ATGGTCTTGA AGACCAGGAA GAACGGAGGG 
TATTACCTCA GCTGCTTGGG CTACCCCGCC TGTAAATCCG CAGTGTGGTT TCCCGATTCG 
GTGCTGGAAG CCAGCAAAGA CGGCTCGGTC TGTGATGTGT GCAGACCCCA TCCGGTGCAC 
AGGTTGAAAC TGAAGTTCAA ACCGGGGAGC CTTCCGGCCA CGATGCCTCT GGAGTTTGTC 
GGCTGCATCG GAGGCTGTGA CGAGACCTTG AGGGAGATCC TGAACCTGAA ATACCTGCAG 
GGCCCACCCA GGCCCGGCCA GACGGCCAGC CAGCGGACTA ACCCTTTACA AGCCAACCAT 
GCTTTGAACC ATGTGGGCGG CCACCAAACC AGCCGCAGCC TCCCGCTGGT GGGCCCCAGG 
GCGCCCTCTG CGGGGGCAGT CAGGGATCCC TCCGCTTCCC TCGTCGACTC TGACTGGAAC 
AGCAACGCCG TCGTGTGCAA CTGTGGGCAG GAAGCCCTAT CGCTGACCGT CCGGAAAGAA 
GGGCCCAACC AAGGCCGGCA GTTCTATAAA TGCAGCGCCG GGACGTGCAA CTTCTTCCTG 
TGGGACGACC GGCAGACAGA GGGCACAAGC AACAGGACGG CCAGGGGCTC TGCCCCACCT 
TCGGGGTCCT TAACAAGGGG GGGGCCAGGC TCGGCACGCT CCCGGAGTGA AGGTGCTGAA 
CCAGGAGGCG GCGGCAACGG CGGTGCCGTC TGCAGATGCG ACCAGCCGGC AGTCACGAGG 
ACGGTCCAGA AAGAGGGACC CAACAAGGGG CGGCCGTTCC ATACCTGCTC CAAGCCCAGA 
GAGCAGCAGT GTGGGTTTTT CCAGTGGGCT GATGAAAACC TGGCACCAGC GCCGGCATCC 
GGCTTTCCTT CAAACCCCTT CACGGGCGAA CGGAGCGCGT GGGGGTCGGG AGCCAAAGCG 
AAAAGGTCCG ACAGCGATTC TTCCCTGAGG GCACCGGCCA CCAAGAGGCA ACGGACCTGC 
GGCCTTTGCC ACCAGCCGGG CCACACCAGG AAGACGTGTC CGCAGAATCG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020670289.1
CDS	665..3718
Translation	MLRMISQTRLFALSGVRLFFCPQKLCFSQAAEGMTFRNIQRVLCVAEKNDAAKGIADILSNSRMRRREGFSKYNKIYECQYHLFGQNVTVVMTSVSGHLLSHDFKLPFRKWHSCNPLVLFDADIEKFCPENYMDIKRTLEREAQQCQALVIWTDGDREGENIGFEIIQVCKAVKPSLQVFRARFSEITPNAIRAACQNLTQPDQNVSDAVDVRQELDLRIGAAFTRFQTLRLQKIFPGVLADQLISYGSCQFPTLGFVVERFKAIEAFVSETFYKIKVTHEHEEGTVDFGWKRNRLFNHTACLVLYHMCMEDPVATVVEVGSRPKSKWRPLPLDTVELEKLASRKLRINAKETMKIAEKLYTQGYISYPRTETNIFPRDLDLSNLVQLQTQDPHWGAFAQRILDQGGPTPRQGNKSDQAHPPIHPTKYGVNLQGNDQRLYEFIVRHFLACCSRDAKGQETTVEIDIAKERFVAHGLMILERNYLEVYPYEKWSDKVIPVYEKGSRFQPTTVEMVDGDTSPPQLLTEADLISLMEKHGIGTDATHAEHIETIKSRMYVGLTPDQRFLPGHLGMGLVEGYDSMGYEMSKPDLRAELEADLKLICEGKKDKSVVLREQIQKYKQVFVEAVQKAKKLDEALSRYFGDVTPVSEQEDIYPAMPDPIRKCPQCNSDMVLKTRKNGGYYLSCLGYPACKSAVWFPDSVLEASKDGSVCDVCRPHPVHRLKLKFKPGSLPATMPLEFVGCIGGCDETLREILNLKYLQGPPRPGQTASQRTNPLQANHALNHVGGHQTSRSLPLVGPRAPSAGAVRDPSASLVDSDWNSNAVVCNCGQEALSLTVRKEGPNQGRQFYKCSAGTCNFFLWDDRQTEGTSNRTARGSAPPSGSLTRGGPGSARSRSEGAEPGGGGNGGAVCRCDQPAVTRTVQKEGPNKGRPFHTCSKPREQQCGFFQWADENLAPAPASGFPSNPFTGERSAWGSGAKAKRSDSDSSLRAPATKRQRTCGLCHQPGHTRKTCPQNR

Target ORF information:

RefSeq Version	XM_020814630.1
Organism	Pogona vitticeps(central bearded dragon)
Definition	Pogona vitticeps topoisomerase (DNA) III alpha (TOP3A), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020814630.1

ORF Insert Sequence:

ATGCTCAGGA TGATCAGTCA GACTAGACTG TTTGCGCTCA GTGGAGTCCG ATTGTTCTTC 
TGTCCCCAGA AACTTTGCTT TTCTCAGGCT GCGGAAGGCA TGACGTTCCG AAACATCCAG 
AGAGTCCTCT GCGTGGCAGA GAAAAATGAT GCTGCCAAAG GAATTGCAGA CATCCTTTCT 
AACAGCAGGA TGAGGAGGAG GGAAGGATTT TCCAAGTATA ACAAGATTTA TGAATGCCAG 
TACCACCTCT TTGGCCAGAA CGTCACGGTG GTGATGACAT CCGTCTCGGG ACACTTGCTC 
TCCCATGACT TCAAGCTACC CTTTCGAAAG TGGCACAGCT GTAACCCACT TGTCCTTTTT 
GATGCTGACA TTGAGAAATT CTGCCCTGAA AACTACATGG ACATTAAGCG AACCCTTGAG 
CGAGAAGCTC AGCAGTGCCA AGCTCTGGTC ATCTGGACAG ACGGCGACCG CGAGGGAGAG 
AACATTGGCT TCGAAATCAT TCAAGTCTGC AAAGCAGTGA AGCCAAGCCT CCAGGTGTTC 
CGCGCCCGCT TCTCTGAGAT CACACCGAAC GCCATAAGAG CGGCCTGCCA GAACCTCACC 
CAGCCAGATC AGAACGTCAG TGATGCCGTC GATGTTAGGC AGGAACTGGA TCTCAGGATT 
GGTGCTGCGT TCACTCGGTT CCAGACTTTG AGGCTCCAGA AAATCTTCCC TGGCGTTTTA 
GCAGACCAGC TGATCAGCTA CGGGAGCTGT CAGTTCCCGA CGCTGGGCTT TGTGGTGGAA 
AGGTTCAAAG CCATCGAAGC CTTCGTTTCC GAGACCTTCT ACAAAATTAA AGTGACTCAC 
GAGCATGAAG AGGGGACGGT GGATTTCGGC TGGAAGAGGA ACCGTCTCTT CAACCATACA 
GCATGCCTCG TCCTTTACCA CATGTGCATG GAGGACCCAG TGGCCACAGT GGTGGAAGTT 
GGAAGCAGAC CAAAGAGCAA ATGGAGACCG CTGCCTCTGG ACACTGTGGA ACTCGAGAAG 
CTGGCTTCCC GTAAACTGAG GATAAATGCT AAAGAAACCA TGAAGATTGC CGAGAAGCTC 
TACACTCAAG GATACATAAG TTACCCCCGA ACAGAAACCA ACATTTTCCC CAGAGACCTC 
GACCTTTCGA ACCTGGTTCA GCTCCAGACA CAAGACCCTC ACTGGGGAGC CTTCGCACAG 
AGGATTCTGG ACCAGGGTGG GCCAACCCCA CGTCAGGGAA ATAAATCGGA TCAGGCCCAT 
CCTCCCATTC ATCCTACAAA ATACGGAGTG AATCTGCAGG GGAACGACCA GCGGCTGTAC 
GAATTCATTG TGCGCCATTT CCTGGCCTGC TGTTCCCGAG ATGCAAAAGG CCAGGAGACG 
ACAGTGGAGA TCGACATAGC CAAAGAGCGC TTCGTCGCTC ACGGGTTGAT GATCCTGGAG 
AGAAATTACT TGGAGGTCTA TCCGTACGAG AAGTGGAGCG ATAAGGTCAT CCCCGTTTAT 
GAGAAGGGGT CTCGCTTTCA GCCCACCACC GTGGAGATGG TCGACGGGGA CACCAGCCCG 
CCGCAGCTGC TGACGGAAGC TGATCTCATT TCCCTCATGG AGAAACACGG CATCGGTACG 
GACGCCACCC ACGCGGAGCA CATCGAAACC ATCAAATCCC GCATGTACGT GGGGCTCACG 
CCGGACCAGC GGTTCCTTCC AGGACATCTA GGGATGGGGC TGGTAGAAGG CTACGATTCC 
ATGGGCTACG AGATGTCCAA GCCTGACCTC CGAGCTGAGC TCGAGGCCGA CCTGAAGCTG 
ATCTGTGAGG GCAAGAAGGA CAAATCCGTG GTGCTGAGGG AGCAAATCCA GAAGTACAAG 
CAGGTCTTTG TAGAAGCGGT GCAGAAGGCC AAGAAGTTGG ATGAAGCGCT GTCTCGGTAT 
TTTGGAGACG TGACTCCTGT TTCAGAACAG GAAGACATTT ACCCCGCCAT GCCCGATCCC 
ATCAGGAAAT GTCCGCAGTG CAACAGCGAC ATGGTCTTGA AGACCAGGAA GAACGGAGGG 
TATTACCTCA GCTGCTTGGG CTACCCCGCC TGTAAATCCG CAGTGTGGTT TCCCGATTCG 
GTGCTGGAAG CCAGCAAAGA CGGCTCGGTC TGTGATGTGT GCAGACCCCA TCCGGTGCAC 
AGGTTGAAAC TGAAGTTCAA ACCGGGGAGC CTTCCGGCCA CGATGCCTCT GGAGTTTGTC 
GGCTGCATCG GAGGCTGTGA CGAGACCTTG AGGGAGATCC TGAACCTGAA ATACCTGCAG 
GGCCCACCCA GGCCCGGCCA GACGGCCAGC CAGCGGACTA ACCCTTTACA AGCCAACCAT 
GCTTTGAACC ATGTGGGCGG CCACCAAACC AGCCGCAGCC TCCCGCTGGT GGGCCCCAGG 
GCGCCCTCTG CGGGGGCAGT CAGGGATCCC TCCGCTTCCC TCGTCGACTC TGACTGGAAC 
AGCAACGCCG TCGTGTGCAA CTGTGGGCAG GAAGCCCTAT CGCTGACCGT CCGGAAAGAA 
GGGCCCAACC AAGGCCGGCA GTTCTATAAA TGCAGCGCCG GGACGTGCAA CTTCTTCCTG 
TGGGACGACC GGCAGACAGA GGGCACAAGC AACAGGACGG CCAGGGGCTC TGCCCCACCT 
TCGGGGTCCT TAACAAGGGG GGGGCCAGGC TCGGCACGCT CCCGGAGTGA AGGTGCTGAA 
CCAGGAGGCG GCGGCAACGG CGGTGCCGTC TGCAGATGCG ACCAGCCGGC AGTCACGAGG 
ACGGTCCAGA AAGAGGGACC CAACAAGGGG CGGCCGTTCC ATACCTGCTC CAAGCCCAGA 
GAGCAGCAGT GTGGGTTTTT CCAGTGGGCT GATGAAAACC TGGCACCAGC GCCGGCATCC 
GGCTTTCCTT CAAACCCCTT CACGGGCGAA CGGAGCGCGT GGGGGTCGGG AGCCAAAGCG 
AAAAGGTCCG ACAGCGATTC TTCCCTGAGG GCACCGGCCA CCAAGAGGCA ACGGACCTGC 
GGCCTTTGCC ACCAGCCGGG CCACACCAGG AAGACGTGTC CGCAGAATCG CTGA