pnkp cDNA ORF clone, Poecilia latipinna(sailfin molly)

Gene
Clones
gRNAs

Summary

Gene Symbol	pnkp
Entrez Gene ID	106965111
Full Name	polynucleotide kinase 3'-phosphatase
Gene Type	protein-coding
Organism	Poecilia latipinna(sailfin molly)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015061017.1	XP_014916503.1	bifunctional polynucleotide phosphatase/kinase

Pathways from BioSystems
Gene Ontology

The following pnkp gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the pnkp cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPo31509	XM_015061017.1 Latest version!	Poecilia latipinna polynucleotide kinase 3'-phosphatase (pnkp), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

pnkp ( XM_015061017.1 ) cDNA ORF clone, Poecilia latipinna(sailfin molly) -> XP_014916503.1 Poecilia latipinna polynucleotide kinase 3'-phosphatase (pnkp), mRNA.

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CloneID	OPo31509
Clone ID Related Accession (Same CDS sequence)	XM_015061017.1
Accession Version	XM_015061017.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1782bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-14
Organism	Poecilia latipinna(sailfin molly)
Product	bifunctional polynucleotide phosphatase/kinase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015112684.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Poecilia latipinna Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGTGTA CGCTGGTTGG ACCCTCTGGG ATCCGGGTTC CACTGGAGGA CGGACGGGCT 
GTGATTCTGG GCCGGGGTCC GGACACTGGG GTGGCCGACA AGAAATGCTC CAGGCACCAG 
GTGAAGGCGG TGGCTTGTTA TGCAGAGCAG GATGTGGTTG TTACTCAGCT TGGGCCAAAT 
CCCAGTTTCC TAAATGGTGA GAAGCTGGGT CGGGGTCAGT CAGGTAAACT TACCCACGGA 
GGCACCCTGT ACCTGGTCAA CCAGAGCCAC CCGTTTACAC TGGACGTCTC TCTGAGCTCC 
AACGGAACAG CTTGCAATAT GCCACGGAGG AACCTGAAAG CCTCAGATAA GACTAAAGGA 
GGTAGAAACA GGAAGGAGGC TGAATCCAGT CCGACTCCAA AACGCAGTAT TAAGGACTTC 
TTTTCCGTCT CTCCCTCTAA GGCTTCTAAA AGGCAGCTGA ACACAGAGAG GGACCAGTCG 
GAGGTGAAGC GACAGAAATT AGATGAGCAG GATGAGGAGG AGAGACTCGC TGAGGAAAAG 
CTAAAGCAGC TGCAGGAGTT TGCAGAAAAG AAGATGACAG AGGAGAATAA AGACACCCAT 
TCTTCCCCGT CGTCATCTCC AGCAAAGGCG TGCATGAAGA GCAGCTGGCA GCAGACAGGA 
AACCTCTTGT TTTACATCGC TGCAGGAGTG AAAGCGAGCG ACAAGATTGC TGGGTTTGAT 
ATTGATGGAT GCATTATCAC CACCAAATCT GGAAAAGTAT TTCCCACCGC ACCTGATGAC 
TGGAAGATTC TGTACCCGGA GATTCAGCCC AGGCTGGCCA GCTTGCTCAA AAAAGGCTAT 
AAGGTTGTTT TCTTCACCAA CCAGCTGGGG ATCGCTAGGG GCAAACTGAT ACCAGAGGTG 
TTCAAGTCTA AAGTGGAGGA CATCCTCACC ACACTGCAGC TGCCTGTGCA GGTGTTTGTC 
GCCAGCGGCC CTGGTGTCTA CAGGAAACCC GTGATGGGAA TGTGGAATCA CCTGTGTGAG 
AAAGCGAATG ACGGGCTGGC TGTCGACAAG ACCCAGAGTT TTTATGTCGG AGATGCTGCA 
GGAAGGCCGG AGAACTGGGC TCCGGGCAAG AAGAAGAAGG ATTTCTCCTG CAGTGACAGA 
TTGTTTGCGC TGAACATTGG GCTTAAGTTC TACACACCAG AAGAATACTT TTTAGGATGG 
AAGAGCGCCC CCTACAGCCT GCCTGACTTT GACCCTCGGA AGCTCGACTC CAGCTGCAGA 
CTGTTCGACC CGCCGTCCGC CTCGCTCACC TGCACCAACA CAGAAGTTAT TGTTGCCGTG 
GGTTACCCTG CAGCTGGTAA ATCCACCTTC TTCCACACCC ACGTTGTTCC GAAGGGCTAC 
GTCTACGTCA ACAGGGACAC GCTCGGCTCC TGGCAGAACT GCGTGTCGGC TTGCGAGCGC 
GCTCTGAAGG AGGGCCGCAG CGTCGCCGTG GACAACACCA ACCCAGACCC AGAGTCTCGC 
AAACGATACT TGGACGTGGC CAAAGCAGCA GGAGTGTCCT GTCGTTGTTT CCTCTTCACT 
GCCACCTTGG AGCAAGCCAA GCACAATAAC AGATTCCGTG AGATGGCTCC ATCCAACAGC 
AAACACGCTA AGGTCAACGA CATGGTTTTC CACAGCTACA AGAAAAACTT TGTGGCTCCA 
AATGTCTCTG AGGGGTTCTC TGAGATCCTT CAAATCCACT TTGTTCCACA CTTCAAGGAC 
AGTCAGTCTG AAACTCTGTT CAGGCAGTTC TCCGAGGGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014916503.1
CDS	234..2015
Translation	MSCTLVGPSGIRVPLEDGRAVILGRGPDTGVADKKCSRHQVKAVACYAEQDVVVTQLGPNPSFLNGEKLGRGQSGKLTHGGTLYLVNQSHPFTLDVSLSSNGTACNMPRRNLKASDKTKGGRNRKEAESSPTPKRSIKDFFSVSPSKASKRQLNTERDQSEVKRQKLDEQDEEERLAEEKLKQLQEFAEKKMTEENKDTHSSPSSSPAKACMKSSWQQTGNLLFYIAAGVKASDKIAGFDIDGCIITTKSGKVFPTAPDDWKILYPEIQPRLASLLKKGYKVVFFTNQLGIARGKLIPEVFKSKVEDILTTLQLPVQVFVASGPGVYRKPVMGMWNHLCEKANDGLAVDKTQSFYVGDAAGRPENWAPGKKKKDFSCSDRLFALNIGLKFYTPEEYFLGWKSAPYSLPDFDPRKLDSSCRLFDPPSASLTCTNTEVIVAVGYPAAGKSTFFHTHVVPKGYVYVNRDTLGSWQNCVSACERALKEGRSVAVDNTNPDPESRKRYLDVAKAAGVSCRCFLFTATLEQAKHNNRFREMAPSNSKHAKVNDMVFHSYKKNFVAPNVSEGFSEILQIHFVPHFKDSQSETLFRQFSEG

Target ORF information:

RefSeq Version	XM_015061017.1
Organism	Poecilia latipinna(sailfin molly)
Definition	Poecilia latipinna polynucleotide kinase 3'-phosphatase (pnkp), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015061017.1

ORF Insert Sequence:

ATGTCGTGTA CGCTGGTTGG ACCCTCTGGG ATCCGGGTTC CACTGGAGGA CGGACGGGCT 
GTGATTCTGG GCCGGGGTCC GGACACTGGG GTGGCCGACA AGAAATGCTC CAGGCACCAG 
GTGAAGGCGG TGGCTTGTTA TGCAGAGCAG GATGTGGTTG TTACTCAGCT TGGGCCAAAT 
CCCAGTTTCC TAAATGGTGA GAAGCTGGGT CGGGGTCAGT CAGGTAAACT TACCCACGGA 
GGCACCCTGT ACCTGGTCAA CCAGAGCCAC CCGTTTACAC TGGACGTCTC TCTGAGCTCC 
AACGGAACAG CTTGCAATAT GCCACGGAGG AACCTGAAAG CCTCAGATAA GACTAAAGGA 
GGTAGAAACA GGAAGGAGGC TGAATCCAGT CCGACTCCAA AACGCAGTAT TAAGGACTTC 
TTTTCCGTCT CTCCCTCTAA GGCTTCTAAA AGGCAGCTGA ACACAGAGAG GGACCAGTCG 
GAGGTGAAGC GACAGAAATT AGATGAGCAG GATGAGGAGG AGAGACTCGC TGAGGAAAAG 
CTAAAGCAGC TGCAGGAGTT TGCAGAAAAG AAGATGACAG AGGAGAATAA AGACACCCAT 
TCTTCCCCGT CGTCATCTCC AGCAAAGGCG TGCATGAAGA GCAGCTGGCA GCAGACAGGA 
AACCTCTTGT TTTACATCGC TGCAGGAGTG AAAGCGAGCG ACAAGATTGC TGGGTTTGAT 
ATTGATGGAT GCATTATCAC CACCAAATCT GGAAAAGTAT TTCCCACCGC ACCTGATGAC 
TGGAAGATTC TGTACCCGGA GATTCAGCCC AGGCTGGCCA GCTTGCTCAA AAAAGGCTAT 
AAGGTTGTTT TCTTCACCAA CCAGCTGGGG ATCGCTAGGG GCAAACTGAT ACCAGAGGTG 
TTCAAGTCTA AAGTGGAGGA CATCCTCACC ACACTGCAGC TGCCTGTGCA GGTGTTTGTC 
GCCAGCGGCC CTGGTGTCTA CAGGAAACCC GTGATGGGAA TGTGGAATCA CCTGTGTGAG 
AAAGCGAATG ACGGGCTGGC TGTCGACAAG ACCCAGAGTT TTTATGTCGG AGATGCTGCA 
GGAAGGCCGG AGAACTGGGC TCCGGGCAAG AAGAAGAAGG ATTTCTCCTG CAGTGACAGA 
TTGTTTGCGC TGAACATTGG GCTTAAGTTC TACACACCAG AAGAATACTT TTTAGGATGG 
AAGAGCGCCC CCTACAGCCT GCCTGACTTT GACCCTCGGA AGCTCGACTC CAGCTGCAGA 
CTGTTCGACC CGCCGTCCGC CTCGCTCACC TGCACCAACA CAGAAGTTAT TGTTGCCGTG 
GGTTACCCTG CAGCTGGTAA ATCCACCTTC TTCCACACCC ACGTTGTTCC GAAGGGCTAC 
GTCTACGTCA ACAGGGACAC GCTCGGCTCC TGGCAGAACT GCGTGTCGGC TTGCGAGCGC 
GCTCTGAAGG AGGGCCGCAG CGTCGCCGTG GACAACACCA ACCCAGACCC AGAGTCTCGC 
AAACGATACT TGGACGTGGC CAAAGCAGCA GGAGTGTCCT GTCGTTGTTT CCTCTTCACT 
GCCACCTTGG AGCAAGCCAA GCACAATAAC AGATTCCGTG AGATGGCTCC ATCCAACAGC 
AAACACGCTA AGGTCAACGA CATGGTTTTC CACAGCTACA AGAAAAACTT TGTGGCTCCA 
AATGTCTCTG AGGGGTTCTC TGAGATCCTT CAAATCCACT TTGTTCCACA CTTCAAGGAC 
AGTCAGTCTG AAACTCTGTT CAGGCAGTTC TCCGAGGGCT GA