MIA3 cDNA ORF clone, Phascolarctos cinereus(koala)

Gene
Clones
gRNAs

Summary

Gene Symbol	MIA3
Entrez Gene ID	110205545
Full Name	MIA family member 3, ER export factor
Gene Type	protein-coding
Organism	Phascolarctos cinereus(koala)

mRNA and Protein(s)

mRNA	Protein	Name
XM_020982207.1	XP_020837866.1	melanoma inhibitory activity protein 3 isoform X1
XM_020982211.1	XP_020837870.1	melanoma inhibitory activity protein 3 isoform X3
XM_020982212.1	XP_020837871.1	melanoma inhibitory activity protein 3 isoform X4
XM_020982208.1	XP_020837867.1	melanoma inhibitory activity protein 3 isoform X1
XM_020982213.1	XP_020837872.1	melanoma inhibitory activity protein 3 isoform X5
XM_020982209.1	XP_020837868.1	melanoma inhibitory activity protein 3 isoform X2

Pathways from BioSystems
Gene Ontology

The following MIA3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MIA3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OPh251846	XM_020982212.1 Latest version!	Phascolarctos cinereus MIA family member 3, ER export factor (MIA3), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OPh251847	XM_020982213.1 Latest version!	Phascolarctos cinereus MIA family member 3, ER export factor (MIA3), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OPh251843	XM_020982207.1 Latest version!	Phascolarctos cinereus MIA family member 3, ER export factor (MIA3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OPh251843	XM_020982208.1 Latest version!	Phascolarctos cinereus MIA family member 3, ER export factor (MIA3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OPh251844	XM_020982209.1 Latest version!	Phascolarctos cinereus MIA family member 3, ER export factor (MIA3), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OPh251845	XM_020982211.1 Latest version!	Phascolarctos cinereus MIA family member 3, ER export factor (MIA3), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MIA3 ( XM_020982212.1 ) cDNA ORF clone, Phascolarctos cinereus(koala) -> XP_020837871.1 Phascolarctos cinereus MIA family member 3, ER export factor (MIA3), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OPh251846
Clone ID Related Accession (Same CDS sequence)	XM_020982212.1
Accession Version	XM_020982212.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2457bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-09
Organism	Phascolarctos cinereus(koala)
Product	melanoma inhibitory activity protein 3 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018343964.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Phascolarctos cinereus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGACGG GGCCCGCCCC GACCGGGCCG TCGGCCGCCA CCACGGGGAC CCCCGAGTTG 
CTGGGCTCCG TGTCCATGTT GTACGCCGCC CTCCTGGCCA AGCTGCTGGA GTTGATTGCT 
ACATTACCTG AGGATATTCG ACCTGGTCCT GATTTTCGTG GAATGCCCTG GGAGCCTATA 
ATTATTACAG TCTTACTGGG AATTGTTTCA CTTGCCATTT TCTTCTGGAG AACGTTTCTT 
TCAGTGAAGA GTAGAGTTTA TCAAGTGACT GAAAAACAGC TTGCTGAGAA GATTAAAACT 
CTTTTACAAG AAAAAACAGA GATTTTAGAA AAAATATCAG AATATGATCA GAAGATCAAA 
AAAGCAAAGG AGTCAGTGAA GGAAACCAAG AAACAAAATA TTAATCTTTC TGATGAAGCA 
GCTGGCCTTA AGGATAAAAT GAGAGGGCTT GAAGAAACAA ACCAAAAACT GGGTGACAAA 
GTGAAAAATC TCCATTCATT GTTGGAAACT GAGAAAGAAC AGAATGTAAA GAAACAGGAC 
ATGATATTGG AAACTCAGAA ATCAATAGAA AAGCTACAAG AAGTTATTGC AATGCATTCT 
GTTGAACTTT CAGAGGTGCA GATAGCCCTT AATGAAGCTA AGTTCAGTGA AGAGAAAGTA 
AAGTCTGAAC TGCATCATGT GCAAGAAGAA AATGCACGAC TCAAAAAGAG AAAGGAGCAG 
CTGCTGCAGG AAGCAGAAGG TTGGAGTGAG CGACACACTG AACTCAGTGA GCAAATCAAA 
TTATATCAGA AGACTCAGAA AGACACCGAA GAAGCCCTTG CTTACAAAGA GAATGAAATT 
GAAGTTTTGA CTAATTGCAT TATGCAGCTG AAACAACTTG ATCTTGATTC TGAATCTGAG 
CGCAAAAATA ATGAAGAAGG AAATGGATGG GATGACTTAG CCAATGGAGA ACTGTCAGAT 
AAAATCACCT CAGATAATCG GAATGAGAAG ATGAAGAGCC AGATTAAACA GATGATGGAT 
GTCTCACGGG TTAAAACTAC ATTAACAATA GTTGAAGAAG ATAGAAACCA ATTGCAGTCC 
AAACTCAGTG ATGAAATAAA AGCTCGGCAT GAATTGGAAG AACAAATTAA GAAGTTGGAA 
CATGACTCTT CTTCTCTGCA GTCAGCCAAA GCACAGTTGG AAAATGAATG TAAAACTCTG 
CAACAAAAAG TGGAGATTCT TAATGAACTC TACCAGCAGA AAGAGATGGT TCTGCAGAAG 
AAGCTCACTC AGGAAGAGTA TGAAAGGCAA GAAAAGGAGC AAAAGCTCAC TGCTGCAGAT 
GAGAAAGTCA GCTTGGTTGC AGAAGAAGTG AAAACTTATA AGCAAAGAAT TCAGGAGATG 
GAGGATGAAT TACAGAAAAC AGAGAGGTCA TATAAGAATC AGATTGCTAG TCATGAGAAG 
AAGGCCCATG ACAACTGGCT CATTGCTCGT GCTGCAGAAA GGGCTCTTGC AGAGGAGAAG 
AGGGAAGCAG CCAACCTGAG ACAGAAATTA ATAGAAGTGA ATCAAAAGAT TGCAATGCTC 
CAAAGACCAA TGATTGTAAA GCCAACTCCA GGAAGACCTG ATCATCAGAT TCCGCCACGG 
AGAGGGCCCC TCAGCCGAGA CGGCTCTTTT GGTCCATCCC CTGTGAGTGG TGGTGCACCC 
TCCCCACCAC TGATGATAGA ACCGCCTATA CGGTCAGTCT CTGCTACTCT TAACCGAAGA 
GATGTACCTA GAAGTGAATT TGGATCTATG GATGGGCCTC CTCTTGGTCC TAGTCTGAGG 
AGGTCATCTG AAGTATCTGG AAGAACGTCT GCCTCTGATT TGGGTCCTGG TCCAGTCCCT 
TTGATGAATA GTGGCCCAAG AAGCTCTTCT CCCTCAACAG TGCTGGATGG CATGCAAACC 
CTTCCCCCAG AGGCTGAAGG CCCCTGTGCT CCTACTGTTC TACCATCAGC TGAGGAGTCA 
GTACCAGTTA ACACTGGTCC CAAAGGCCAC CCACCATTCC CAGGAATGCC CGTCATGTCA 
CCAATAGGAG GACCTCCACC ACCACCTCTT CGATTTGGAC CACCTCCTCC ACTACGAGGA 
CCTTATGGGC CTCGACCTCT TCCTCCACCT CTTGTTCCTG GTGGTCCTCC ACCTCCAAGC 
TTTCGGGAGT ACTTACATGG CCTTCCACCA GGAGTAAGGG ACCTACCTCC TGACCCTCGA 
GAGTATATAC GTGGACACCC ACCTTTTGGA CCTCTAGGCC CTCCTGGCCC AAGAGAGTAC 
CTTCCTCCTG GCCCACGACT GCCGCCTCCA GCCCATGGTC CAAGGGACTA CCCACCTCCA 
CCTTTTACAA GAGACTTGAC ACCTTCAGGT TCTAGAGACT GTGCCCCCCC TCATCCACCT 
CATGCCTCTC AAGGAAGCAC ACAGGACTAT GCACAGGCTC CAGAACAGAG CCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020837871.1
CDS	189..2645
Translation	METGPAPTGPSAATTGTPELLGSVSMLYAALLAKLLELIATLPEDIRPGPDFRGMPWEPIIITVLLGIVSLAIFFWRTFLSVKSRVYQVTEKQLAEKIKTLLQEKTEILEKISEYDQKIKKAKESVKETKKQNINLSDEAAGLKDKMRGLEETNQKLGDKVKNLHSLLETEKEQNVKKQDMILETQKSIEKLQEVIAMHSVELSEVQIALNEAKFSEEKVKSELHHVQEENARLKKRKEQLLQEAEGWSERHTELSEQIKLYQKTQKDTEEALAYKENEIEVLTNCIMQLKQLDLDSESERKNNEEGNGWDDLANGELSDKITSDNRNEKMKSQIKQMMDVSRVKTTLTIVEEDRNQLQSKLSDEIKARHELEEQIKKLEHDSSSLQSAKAQLENECKTLQQKVEILNELYQQKEMVLQKKLTQEEYERQEKEQKLTAADEKVSLVAEEVKTYKQRIQEMEDELQKTERSYKNQIASHEKKAHDNWLIARAAERALAEEKREAANLRQKLIEVNQKIAMLQRPMIVKPTPGRPDHQIPPRRGPLSRDGSFGPSPVSGGAPSPPLMIEPPIRSVSATLNRRDVPRSEFGSMDGPPLGPSLRRSSEVSGRTSASDLGPGPVPLMNSGPRSSSPSTVLDGMQTLPPEAEGPCAPTVLPSAEESVPVNTGPKGHPPFPGMPVMSPIGGPPPPPLRFGPPPPLRGPYGPRPLPPPLVPGGPPPPSFREYLHGLPPGVRDLPPDPREYIRGHPPFGPLGPPGPREYLPPGPRLPPPAHGPRDYPPPPFTRDLTPSGSRDCAPPHPPHASQGSTQDYAQAPEQSP

Target ORF information:

RefSeq Version	XM_020982212.1
Organism	Phascolarctos cinereus(koala)
Definition	Phascolarctos cinereus MIA family member 3, ER export factor (MIA3), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020982212.1

ORF Insert Sequence:

ATGGAGACGG GGCCCGCCCC GACCGGGCCG TCGGCCGCCA CCACGGGGAC CCCCGAGTTG 
CTGGGCTCCG TGTCCATGTT GTACGCCGCC CTCCTGGCCA AGCTGCTGGA GTTGATTGCT 
ACATTACCTG AGGATATTCG ACCTGGTCCT GATTTTCGTG GAATGCCCTG GGAGCCTATA 
ATTATTACAG TCTTACTGGG AATTGTTTCA CTTGCCATTT TCTTCTGGAG AACGTTTCTT 
TCAGTGAAGA GTAGAGTTTA TCAAGTGACT GAAAAACAGC TTGCTGAGAA GATTAAAACT 
CTTTTACAAG AAAAAACAGA GATTTTAGAA AAAATATCAG AATATGATCA GAAGATCAAA 
AAAGCAAAGG AGTCAGTGAA GGAAACCAAG AAACAAAATA TTAATCTTTC TGATGAAGCA 
GCTGGCCTTA AGGATAAAAT GAGAGGGCTT GAAGAAACAA ACCAAAAACT GGGTGACAAA 
GTGAAAAATC TCCATTCATT GTTGGAAACT GAGAAAGAAC AGAATGTAAA GAAACAGGAC 
ATGATATTGG AAACTCAGAA ATCAATAGAA AAGCTACAAG AAGTTATTGC AATGCATTCT 
GTTGAACTTT CAGAGGTGCA GATAGCCCTT AATGAAGCTA AGTTCAGTGA AGAGAAAGTA 
AAGTCTGAAC TGCATCATGT GCAAGAAGAA AATGCACGAC TCAAAAAGAG AAAGGAGCAG 
CTGCTGCAGG AAGCAGAAGG TTGGAGTGAG CGACACACTG AACTCAGTGA GCAAATCAAA 
TTATATCAGA AGACTCAGAA AGACACCGAA GAAGCCCTTG CTTACAAAGA GAATGAAATT 
GAAGTTTTGA CTAATTGCAT TATGCAGCTG AAACAACTTG ATCTTGATTC TGAATCTGAG 
CGCAAAAATA ATGAAGAAGG AAATGGATGG GATGACTTAG CCAATGGAGA ACTGTCAGAT 
AAAATCACCT CAGATAATCG GAATGAGAAG ATGAAGAGCC AGATTAAACA GATGATGGAT 
GTCTCACGGG TTAAAACTAC ATTAACAATA GTTGAAGAAG ATAGAAACCA ATTGCAGTCC 
AAACTCAGTG ATGAAATAAA AGCTCGGCAT GAATTGGAAG AACAAATTAA GAAGTTGGAA 
CATGACTCTT CTTCTCTGCA GTCAGCCAAA GCACAGTTGG AAAATGAATG TAAAACTCTG 
CAACAAAAAG TGGAGATTCT TAATGAACTC TACCAGCAGA AAGAGATGGT TCTGCAGAAG 
AAGCTCACTC AGGAAGAGTA TGAAAGGCAA GAAAAGGAGC AAAAGCTCAC TGCTGCAGAT 
GAGAAAGTCA GCTTGGTTGC AGAAGAAGTG AAAACTTATA AGCAAAGAAT TCAGGAGATG 
GAGGATGAAT TACAGAAAAC AGAGAGGTCA TATAAGAATC AGATTGCTAG TCATGAGAAG 
AAGGCCCATG ACAACTGGCT CATTGCTCGT GCTGCAGAAA GGGCTCTTGC AGAGGAGAAG 
AGGGAAGCAG CCAACCTGAG ACAGAAATTA ATAGAAGTGA ATCAAAAGAT TGCAATGCTC 
CAAAGACCAA TGATTGTAAA GCCAACTCCA GGAAGACCTG ATCATCAGAT TCCGCCACGG 
AGAGGGCCCC TCAGCCGAGA CGGCTCTTTT GGTCCATCCC CTGTGAGTGG TGGTGCACCC 
TCCCCACCAC TGATGATAGA ACCGCCTATA CGGTCAGTCT CTGCTACTCT TAACCGAAGA 
GATGTACCTA GAAGTGAATT TGGATCTATG GATGGGCCTC CTCTTGGTCC TAGTCTGAGG 
AGGTCATCTG AAGTATCTGG AAGAACGTCT GCCTCTGATT TGGGTCCTGG TCCAGTCCCT 
TTGATGAATA GTGGCCCAAG AAGCTCTTCT CCCTCAACAG TGCTGGATGG CATGCAAACC 
CTTCCCCCAG AGGCTGAAGG CCCCTGTGCT CCTACTGTTC TACCATCAGC TGAGGAGTCA 
GTACCAGTTA ACACTGGTCC CAAAGGCCAC CCACCATTCC CAGGAATGCC CGTCATGTCA 
CCAATAGGAG GACCTCCACC ACCACCTCTT CGATTTGGAC CACCTCCTCC ACTACGAGGA 
CCTTATGGGC CTCGACCTCT TCCTCCACCT CTTGTTCCTG GTGGTCCTCC ACCTCCAAGC 
TTTCGGGAGT ACTTACATGG CCTTCCACCA GGAGTAAGGG ACCTACCTCC TGACCCTCGA 
GAGTATATAC GTGGACACCC ACCTTTTGGA CCTCTAGGCC CTCCTGGCCC AAGAGAGTAC 
CTTCCTCCTG GCCCACGACT GCCGCCTCCA GCCCATGGTC CAAGGGACTA CCCACCTCCA 
CCTTTTACAA GAGACTTGAC ACCTTCAGGT TCTAGAGACT GTGCCCCCCC TCATCCACCT 
CATGCCTCTC AAGGAAGCAC ACAGGACTAT GCACAGGCTC CAGAACAGAG CCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MIA3 ( XM_020982213.1 ) cDNA ORF clone, Phascolarctos cinereus(koala) -> XP_020837872.1 Phascolarctos cinereus MIA family member 3, ER export factor (MIA3), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OPh251847
Clone ID Related Accession (Same CDS sequence)	XM_020982213.1
Accession Version	XM_020982213.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2385bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-09
Organism	Phascolarctos cinereus(koala)
Product	melanoma inhibitory activity protein 3 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018343964.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Phascolarctos cinereus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGACGG GGCCCGCCCC GACCGGGCCG TCGGCCGCCA CCACGGGGAC CCCCGAGTTG 
CTGGGCTCCG TGTCCATGTT GTACGCCGCC CTCCTGGCCA AGCTGCTGGA GTTGATTGCT 
ACATTACCTG AGGATATTCG ACCTGGTCCT GATTTTCGTG GAATGCCCTG GGAGCCTATA 
ATTATTACAG TCTTACTGGG AATTGTTTCA CTTGCCATTT TCTTCTGGAG AACGTTTCTT 
TCAGTGAAGA GTAGAGTTTA TCAAGTGACT GAAAAACAGC TTGCTGAGAA GATTAAAACT 
CTTTTACAAG AAAAAACAGA GATTTTAGAA AAAATATCAG AATATGATCA GAAGATCAAA 
AAAGCAAAGG AGTCAGTGAA GGAAACCAAG AAACAAAATA TTAATCTTTC TGATGAAGCA 
GCTGGCCTTA AGGATAAAAT GAGAGGGCTT GAAGAAACAA ACCAAAAACT GGGTGACAAA 
GTGAAAAATC TCCATTCATT GTTGGAAACT GAGAAAGAAC AGAATGTAAA GAAACAGGAC 
ATGATATTGG AAACTCAGAA ATCAATAGAA AAGCTACAAG AAGTTATTGC AATGCATTCT 
GTTGAACTTT CAGAGGTGCA GATAGCCCTT AATGAAGCTA AGTTCAGTGA AGAGAAAGTA 
AAGTCTGAAC TGCATCATGT GCAAGAAGAA AATGCACGAC TCAAAAAGAG AAAGGAGCAG 
CTGCTGCAGG AAGCAGAAGG TTGGAGTGAG CGACACACTG AACTCAGTGA GCAAATCAAA 
TTATATCAGA AGACTCAGAA AGACACCGAA GAAGCCCTTG CTTACAAAGA GAATGAAATT 
GAAGTTTTGA CTAATTGCAT TATGCAGCTG AAACAACTTG ATCTTGATTC TGAATCTGAG 
CGCAAAAATA ATGAAGAAGG AAATGGATGG GATGACTTAG CCAATGGAGA ACTGTCAGAT 
AAAATCACCT CAGATAATCG GAATGAGAAG ATGAAGAGCC AGATTAAACA GATGATGGAT 
GTCTCACGGG TTAAAACTAC ATTAACAATA GTTGAAGAAG ATAGAAACCA ATTGCAGTCC 
AAACTCAGTG ATGAAATAAA AGCTCGGCAT GAATTGGAAG AACAAATTAA GAAGTTGGAA 
CATGACTCTT CTTCTCTGCA GTCAGCCAAA GCACAGTTGG AAAATGAATG TAAAACTCTG 
CAACAAAAAG TGGAGATTCT TAATGAACTC TACCAGCAGA AAGAGATGGT TCTGCAGAAG 
AAGCTCACTC AGGAAGAGTA TGAAAGGCAA GAAAAGGAGC AAAAGCTCAC TGCTGCAGAT 
GAGAAAGTCA GCTTGGTTGC AGAAGAAGTG AAAACTTATA AGCAAAGAAT TCAGGAGATG 
GAGGATGAAT TACAGAAAAC AGAGAGGTCA TATAAGAATC AGATTGCTAG TCATGAGAAG 
AAGGCCCATG ACAACTGGCT CATTGCTCGT GCTGCAGAAA GGGCTCTTGC AGAGGAGAAG 
AGGGAAGCAG CCAACCTGAG ACAGAAATTA ATAGAAGTGA ATCAAAAGAT TGCAATGCTC 
CAAAGACCAA TGATTGTAAA GCCAACTCCA GGAAGACCTG ATCATCAGAT TCCGCCACGG 
AGAGGGCCCC TCAGCCGAGA CGGCTCTTTT GGTCCATCCC CTGTGAGTGG TGGTGCACCC 
TCCCCACCAC TGATGATAGA ACCGCCTATA CGGTCAGTCT CTGCTACTCT TAACCGAAGA 
GATGTACCTA GAAGTGAATT TGGATCTATG GATGGGCCTC CTCTTGGTCC TAGTCTGAGG 
AGGTCATCTG AAGTATCTGG AAGAACGTCT GCCTCTGATT TGGGTCCTGG TCCAGTCCCT 
TTGATGAATA GTGGCCCAAG AAGCTCTTCT CCCTCAACAG TGCTGGATGG CATGGTTAAC 
ACTGGTCCCA AAGGCCACCC ACCATTCCCA GGAATGCCCG TCATGTCACC AATAGGAGGA 
CCTCCACCAC CACCTCTTCG ATTTGGACCA CCTCCTCCAC TACGAGGACC TTATGGGCCT 
CGACCTCTTC CTCCACCTCT TGTTCCTGGT GGTCCTCCAC CTCCAAGCTT TCGGGAGTAC 
TTACATGGCC TTCCACCAGG AGTAAGGGAC CTACCTCCTG ACCCTCGAGA GTATATACGT 
GGACACCCAC CTTTTGGACC TCTAGGCCCT CCTGGCCCAA GAGAGTACCT TCCTCCTGGC 
CCACGACTGC CGCCTCCAGC CCATGGTCCA AGGGACTACC CACCTCCACC TTTTACAAGA 
GACTTGACAC CTTCAGGTTC TAGAGACTGT GCCCCCCCTC ATCCACCTCA TGCCTCTCAA 
GGAAGCACAC AGGACTATGC ACAGGCTCCA GAACAGAGCC CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020837872.1
CDS	187..2571
Translation	METGPAPTGPSAATTGTPELLGSVSMLYAALLAKLLELIATLPEDIRPGPDFRGMPWEPIIITVLLGIVSLAIFFWRTFLSVKSRVYQVTEKQLAEKIKTLLQEKTEILEKISEYDQKIKKAKESVKETKKQNINLSDEAAGLKDKMRGLEETNQKLGDKVKNLHSLLETEKEQNVKKQDMILETQKSIEKLQEVIAMHSVELSEVQIALNEAKFSEEKVKSELHHVQEENARLKKRKEQLLQEAEGWSERHTELSEQIKLYQKTQKDTEEALAYKENEIEVLTNCIMQLKQLDLDSESERKNNEEGNGWDDLANGELSDKITSDNRNEKMKSQIKQMMDVSRVKTTLTIVEEDRNQLQSKLSDEIKARHELEEQIKKLEHDSSSLQSAKAQLENECKTLQQKVEILNELYQQKEMVLQKKLTQEEYERQEKEQKLTAADEKVSLVAEEVKTYKQRIQEMEDELQKTERSYKNQIASHEKKAHDNWLIARAAERALAEEKREAANLRQKLIEVNQKIAMLQRPMIVKPTPGRPDHQIPPRRGPLSRDGSFGPSPVSGGAPSPPLMIEPPIRSVSATLNRRDVPRSEFGSMDGPPLGPSLRRSSEVSGRTSASDLGPGPVPLMNSGPRSSSPSTVLDGMVNTGPKGHPPFPGMPVMSPIGGPPPPPLRFGPPPPLRGPYGPRPLPPPLVPGGPPPPSFREYLHGLPPGVRDLPPDPREYIRGHPPFGPLGPPGPREYLPPGPRLPPPAHGPRDYPPPPFTRDLTPSGSRDCAPPHPPHASQGSTQDYAQAPEQSP

Target ORF information:

RefSeq Version	XM_020982213.1
Organism	Phascolarctos cinereus(koala)
Definition	Phascolarctos cinereus MIA family member 3, ER export factor (MIA3), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020982213.1

ORF Insert Sequence:

ATGGAGACGG GGCCCGCCCC GACCGGGCCG TCGGCCGCCA CCACGGGGAC CCCCGAGTTG 
CTGGGCTCCG TGTCCATGTT GTACGCCGCC CTCCTGGCCA AGCTGCTGGA GTTGATTGCT 
ACATTACCTG AGGATATTCG ACCTGGTCCT GATTTTCGTG GAATGCCCTG GGAGCCTATA 
ATTATTACAG TCTTACTGGG AATTGTTTCA CTTGCCATTT TCTTCTGGAG AACGTTTCTT 
TCAGTGAAGA GTAGAGTTTA TCAAGTGACT GAAAAACAGC TTGCTGAGAA GATTAAAACT 
CTTTTACAAG AAAAAACAGA GATTTTAGAA AAAATATCAG AATATGATCA GAAGATCAAA 
AAAGCAAAGG AGTCAGTGAA GGAAACCAAG AAACAAAATA TTAATCTTTC TGATGAAGCA 
GCTGGCCTTA AGGATAAAAT GAGAGGGCTT GAAGAAACAA ACCAAAAACT GGGTGACAAA 
GTGAAAAATC TCCATTCATT GTTGGAAACT GAGAAAGAAC AGAATGTAAA GAAACAGGAC 
ATGATATTGG AAACTCAGAA ATCAATAGAA AAGCTACAAG AAGTTATTGC AATGCATTCT 
GTTGAACTTT CAGAGGTGCA GATAGCCCTT AATGAAGCTA AGTTCAGTGA AGAGAAAGTA 
AAGTCTGAAC TGCATCATGT GCAAGAAGAA AATGCACGAC TCAAAAAGAG AAAGGAGCAG 
CTGCTGCAGG AAGCAGAAGG TTGGAGTGAG CGACACACTG AACTCAGTGA GCAAATCAAA 
TTATATCAGA AGACTCAGAA AGACACCGAA GAAGCCCTTG CTTACAAAGA GAATGAAATT 
GAAGTTTTGA CTAATTGCAT TATGCAGCTG AAACAACTTG ATCTTGATTC TGAATCTGAG 
CGCAAAAATA ATGAAGAAGG AAATGGATGG GATGACTTAG CCAATGGAGA ACTGTCAGAT 
AAAATCACCT CAGATAATCG GAATGAGAAG ATGAAGAGCC AGATTAAACA GATGATGGAT 
GTCTCACGGG TTAAAACTAC ATTAACAATA GTTGAAGAAG ATAGAAACCA ATTGCAGTCC 
AAACTCAGTG ATGAAATAAA AGCTCGGCAT GAATTGGAAG AACAAATTAA GAAGTTGGAA 
CATGACTCTT CTTCTCTGCA GTCAGCCAAA GCACAGTTGG AAAATGAATG TAAAACTCTG 
CAACAAAAAG TGGAGATTCT TAATGAACTC TACCAGCAGA AAGAGATGGT TCTGCAGAAG 
AAGCTCACTC AGGAAGAGTA TGAAAGGCAA GAAAAGGAGC AAAAGCTCAC TGCTGCAGAT 
GAGAAAGTCA GCTTGGTTGC AGAAGAAGTG AAAACTTATA AGCAAAGAAT TCAGGAGATG 
GAGGATGAAT TACAGAAAAC AGAGAGGTCA TATAAGAATC AGATTGCTAG TCATGAGAAG 
AAGGCCCATG ACAACTGGCT CATTGCTCGT GCTGCAGAAA GGGCTCTTGC AGAGGAGAAG 
AGGGAAGCAG CCAACCTGAG ACAGAAATTA ATAGAAGTGA ATCAAAAGAT TGCAATGCTC 
CAAAGACCAA TGATTGTAAA GCCAACTCCA GGAAGACCTG ATCATCAGAT TCCGCCACGG 
AGAGGGCCCC TCAGCCGAGA CGGCTCTTTT GGTCCATCCC CTGTGAGTGG TGGTGCACCC 
TCCCCACCAC TGATGATAGA ACCGCCTATA CGGTCAGTCT CTGCTACTCT TAACCGAAGA 
GATGTACCTA GAAGTGAATT TGGATCTATG GATGGGCCTC CTCTTGGTCC TAGTCTGAGG 
AGGTCATCTG AAGTATCTGG AAGAACGTCT GCCTCTGATT TGGGTCCTGG TCCAGTCCCT 
TTGATGAATA GTGGCCCAAG AAGCTCTTCT CCCTCAACAG TGCTGGATGG CATGGTTAAC 
ACTGGTCCCA AAGGCCACCC ACCATTCCCA GGAATGCCCG TCATGTCACC AATAGGAGGA 
CCTCCACCAC CACCTCTTCG ATTTGGACCA CCTCCTCCAC TACGAGGACC TTATGGGCCT 
CGACCTCTTC CTCCACCTCT TGTTCCTGGT GGTCCTCCAC CTCCAAGCTT TCGGGAGTAC 
TTACATGGCC TTCCACCAGG AGTAAGGGAC CTACCTCCTG ACCCTCGAGA GTATATACGT 
GGACACCCAC CTTTTGGACC TCTAGGCCCT CCTGGCCCAA GAGAGTACCT TCCTCCTGGC 
CCACGACTGC CGCCTCCAGC CCATGGTCCA AGGGACTACC CACCTCCACC TTTTACAAGA 
GACTTGACAC CTTCAGGTTC TAGAGACTGT GCCCCCCCTC ATCCACCTCA TGCCTCTCAA 
GGAAGCACAC AGGACTATGC ACAGGCTCCA GAACAGAGCC CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MIA3 ( XM_020982207.1 ) cDNA ORF clone, Phascolarctos cinereus(koala) -> XP_020837866.1 Phascolarctos cinereus MIA family member 3, ER export factor (MIA3), transcript variant X1, mRNA.

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CloneID	OPh251843
Clone ID Related Accession (Same CDS sequence)	XM_020982207.1 , XM_020982208.1
Accession Version	XM_020982207.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6081bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-09
Organism	Phascolarctos cinereus(koala)
Product	melanoma inhibitory activity protein 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018343964.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Phascolarctos cinereus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCAG CGCCGCGTCT GCCCTTCTGT CTCCTCGTTC TCCTCTTCTC CACTGGGCCG 
CGGCTGGGTC AAGCGAACCG AAGCGGGGAC CGGCGCTTCG CGGAGCTGAA ACGCTGCTCG 
GACGAGGAGT GCAGCATGTT GATGTACCGA GGTAGAGCTC TGAAGGATTT CACAGGTCCA 
GACTGTCGCT TTGTAAATTT CAAAGAAGGG GAAGCTGTAT ATGTCTATTA CAAATTAGTA 
GGAAGATCAC CTGAACTTTG GGCTGGAAGT GTTGGCAGTG TTTTTGGGTA CTTTCCAAAG 
GATCTAATTG AAGTAAATCA TCAGTATTCT GAACAAGAAT TAGAACTACC AACAGATGAA 
ACAGATTTTG TTTGTTTTGA TGGAGGAACG GATAATTTTG ACAACTATGA TGTTGAAGAT 
CTTTTAGGAT TTGGGGGAGA AACTGTAAGA GGCAAAGGGG AAGATGAATT GAACACTTCA 
GGAACTGAAA GCTCTGAGAC ACCTACAGAA GAAATGGAGG AGGAGCATCC TAAGAAGGTA 
AATATTGAAG AACCCCTTGA AGTATCCAAG GATGGCAATG ATGAGCCTGA AATAGCAGTG 
GACAGTAAAG TTCTTGCAAA TTCGGAAGAA AACAGCTTAC CTCCTGAAAA CACTAAGAAT 
CTAGAGGAAG AATTTGAAAC ACAGAGGCCC CCAGTCCATG TAAATAGTCA TGCAGATAAT 
CCTCAGGGAA ACCAACCTTT CCTTGAGCCC TTAGAAGAAA TACTGCAAGA TAAATTAAAA 
GTGCCAGAGA GTGAGAATGC CAAAAATATC AATATTTCTC AGCTTTCCAA GGATTGGGAG 
GAAATTGACG CCTATAAACT TTTGAAAAAG GAAATTAATT TGGATTTGAA GACAAAATTT 
GGCTCAACTG CAGATGCACT TATATCTGAT GATGAAACAA CTAGGCTTGT TACTTCATTA 
GAAGATGAAT TTGATGAGGA ATTGGATACT GATGATTCCA GACTTGAGGA AGAGGACAAT 
GAAAGCTTTG ATGAACCACC ATTACTAACT TTTACAAATG GAGAAGATGG GAAAACACCA 
GGGAACACAG TAGTTGAGAA ATATTCAACA GGTGAAAATA CTGAAGGAGA CCCTGATGAA 
ACAGCTCAGC TTAGGACGGG TGAAAAGAAT GATAAAAATA TACTGAAAGC CTTAGGAGAT 
ACTATTTTAT CTATTGTTGG TGGAGGTGAA GAAGAAAGAG ATGCCACAGA TTTGGATGGC 
TCTGATTTAG ATGAAGAAAA GGTAGAAGAT AATGAAAAAG TCCTAACTAG TAAATGGGAA 
GAAGACAGCA CTCCTTTGAC TATGGAAGTG CCTGTAACAA ATCATGACAA AAACCTAGAC 
GATGCCCTTG AAATTGGCAT GGCAACTGAA ATTAAGGAAA ATAGTGAGAG AATACAGTCA 
TCCACAGAAG AATTCATGAA GAATGGAATG GAATTAGGAG ATGAAGACAT TTTTATAAAA 
AGTCCTACTC ATGAAACTGA CCTTAAACTT AATAATCCTG CTGAAAAAAG AGAAGACACT 
TTAAAATCAC CCCTTAATGA TGTGGGAGGT GACCTAAAGG GAACCATTAT TCACCAGACC 
ACAAAAGGGG AAAAGTCTGG AGAGAAGGTT TTAGATGACA ACTTAGAGAG TGATCTTGTA 
CATAAAACTC TCTGGAAGAA AATGAAAGAC AAAAATACTG AACAGAAATC TATAAATACT 
AAACCACTGT TAGAAGATGA ACAACAAAAT GCATCCAAAG GAAACGTGGA TATAATAGGT 
ATTTCAGAAA ATGAACAAGA GCAGGAAATG TTTCCAGTGA AGATGCAAGG AAAGGAATCC 
ATAGAGCTAT CTGGGCTTAG AACTCAAAAT CAAGTCAACA TTTCTTCTGT AGATCAAACT 
GATTTACCAA GAGAAAAGGT GGAGGAGAAT CTTATGTTGG GAGAAAATTT AATGGACCAG 
CAGGAAAAAG ATATGAAAAC TGAGATTAGC AAGAAGCTGA TTGAAAAGAT CAGGCATTCT 
GAAAGAGAGA TTCGAGATCA ACTTTCCAAA GATGGAAGCA AAGAAATGGA GAGCACACAA 
AGTAAAGACA AAGATATTCC TCAGGATGGA GAAACAAATT ATTTCAAAGA AAACGTAGGC 
CAGCCCCTTA TCATAGAGAC AACAGAATTC CAGACAGAAA GTGATGATTT CTATACAGCT 
GAGGATTTGT TAGAAGATGA AAATGCTATA AGCGCTGCTG CTAGTGACCA GAGCAGTAGG 
ATAAATGTTG ATGTGCAAAT GAGAGAAAAA GCTACTTCAG GAGTCACTAA TCTGATTCAT 
ACCACGGAAG ACACCAAAGA GAAAACAAGT ATGATTTTGG AAACTGAGGG AGTAGATAGC 
AGCTTGCAAG AGTCAGGCAG GACTATGCCC AAAGAGGTAA CTGCAAAGGT TGGAGAATCT 
ATTAAAATAG CTATAGGGCA GCAAGAAAAG AATCCCTTCA AAAAAGATGA AAAACCCACA 
TCCTCTGCAA TTAGTGACTC TTCTGGCAAG GATGAAAATC AGGCTCAAGA TTTAGTTGAA 
GAGACTAATT ACTTTGAAAA AGAAGATGGT CCAAAAAACA AAAAAGACTT CAGTTACACA 
GAAAGGTCTT GGCCAGATGA TTTCTCCCAA GAGGACCTTG AATATTCACA GAATTTCACT 
GATGCAGAAA GCCAAACAAA TCAGCAATCT GATTCTACAG AGCTTGATGA TAACTTGGTA 
TATAAAAAAG CTGTTAAGTC AGAATATAGT GACACAATAA AGCAACTGCC TATCATTAAA 
GGCTTTCTTG ATGAAAAACG AGTAGAAAGA TTAGAAAAGT ACCTTGGGTC TGAGAATGTC 
CTCTTAATGG AATCCACATT CCATGACATG GAAAGTAAGC TAGAGCTTGC TCAAAAGGAG 
AGTCTGGCAT ATAATATGGA AAAAGCCCTG GAAGATGTCT TTCGCTCTTC AGAATCAAAC 
ATCCTTGATA TGGTAGAGAA AATGTTGGAT TCAAGAGTGG CAGAAAACAA GGAACTGGGC 
ATGAAGGAGA TAGACATGTT TGATGAAGAG GCTGCTCTGC TGGATGATAT TCAGGACCTG 
ATCTATTTTG TCAGATACAA ACACCCTTTG GTAGAAGAGA GTGTACCACT AGCAGCTGCT 
GAGCCTCCAA AACAAAGTTC TGATGTGCCT CTTGCAGTAT TTCCTATAGA ACAAAAAACA 
GAACCCAAAG GAGACCAGAG AAGAAGGTTT TCAGTGAATG AAGGAGATAC ATTGGCACCA 
GAGGAAAACA AGAGGCCACC AGAGAGTATA GAAATAAAAG TTCATAAAGT GGAAGTACCA 
GAGGAGGATA TGGACATCTC AGAAAAGGAT GATCTTTTGG ATTCAGGAGA GATCAGAGAT 
ATCAGTGACT CAGAAGATAG ACTACTGGTT AAAGAGTCCT CCCCAGACCA AAGTATTGTG 
GAAGATTTGG CTAGAGGAAT GGTTTCAGTA GAAGATACTC CTTTGGATAC AAAACAACAT 
CCTCTTGAAA TAATTTCCAC AGAAGAAACT CCTTTAGATA CAGAACAACA TCCTCTAGGT 
ACAGTTTCCA CAGAAGAGAC TCCTTTGGAT ACAGAACAAC AACTAGAAAC AAATCCAGAT 
GTGCCACTGA TTCTTGCAGC TATGAAGAGT GCAAGTCTTG TAGCAAAAGA AAGCATGAAA 
CAATATACTG AGATGTTGAT TGCTACATTA CCTGAGGATA TTCGACCTGG TCCTGATTTT 
CGTGGAATGC CCTGGGAGCC TATAATTATT ACAGTCTTAC TGGGAATTGT TTCACTTGCC 
ATTTTCTTCT GGAGAACGTT TCTTTCAGTG AAGAGTAGAG TTTATCAAGT GACTGAAAAA 
CAGCTTGCTG AGAAGATTAA AACTCTTTTA CAAGAAAAAA CAGAGATTTT AGAAAAAATA 
TCAGAATATG ATCAGAAGAT CAAAAAAGCA AAGGAGTCAG TGAAGGAAAC CAAGAAACAA 
AATATTAATC TTTCTGATGA AGCAGCTGGC CTTAAGGATA AAATGAGAGG GCTTGAAGAA 
ACAAACCAAA AACTGGGTGA CAAAGTGAAA AATCTCCATT CATTGTTGGA AACTGAGAAA 
GAACAGAATG TAAAGAAACA GGACATGATA TTGGAAACTC AGAAATCAAT AGAAAAGCTA 
CAAGAAGTTA TTGCAATGCA TTCTGTTGAA CTTTCAGAGG TGCAGATAGC CCTTAATGAA 
GCTAAGTTCA GTGAAGAGAA AGTAAAGTCT GAACTGCATC ATGTGCAAGA AGAAAATGCA 
CGACTCAAAA AGAGAAAGGA GCAGCTGCTG CAGGAAGCAG AAGGTTGGAG TGAGCGACAC 
ACTGAACTCA GTGAGCAAAT CAAATTATAT CAGAAGACTC AGAAAGACAC CGAAGAAGCC 
CTTGCTTACA AAGAGAATGA AATTGAAGTT TTGACTAATT GCATTATGCA GCTGAAACAA 
CTTGATCTTG ATTCTGAATC TGAGCGCAAA AATAATGAAG AAGGAAATGG ATGGGATGAC 
TTAGCCAATG GAGAACTGTC AGATAAAATC ACCTCAGATA ATCGGAATGA GAAGATGAAG 
AGCCAGATTA AACAGATGAT GGATGTCTCA CGGGTTAAAA CTACATTAAC AATAGTTGAA 
GAAGATAGAA ACCAATTGCA GTCCAAACTC AGTGATGAAA TAAAAGCTCG GCATGAATTG 
GAAGAACAAA TTAAGAAGTT GGAACATGAC TCTTCTTCTC TGCAGTCAGC CAAAGCACAG 
TTGGAAAATG AATGTAAAAC TCTGCAACAA AAAGTGGAGA TTCTTAATGA ACTCTACCAG 
CAGAAAGAGA TGGTTCTGCA GAAGAAGCTC ACTCAGGAAG AGTATGAAAG GCAAGAAAAG 
GAGCAAAAGC TCACTGCTGC AGATGAGAAA GTCAGCTTGG TTGCAGAAGA AGTGAAAACT 
TATAAGCAAA GAATTCAGGA GATGGAGGAT GAATTACAGA AAACAGAGAG GTCATATAAG 
AATCAGATTG CTAGTCATGA GAAGAAGGCC CATGACAACT GGCTCATTGC TCGTGCTGCA 
GAAAGGGCTC TTGCAGAGGA GAAGAGGGAA GCAGCCAACC TGAGACAGAA ATTAATAGAA 
GTGAATCAAA AGATTGCAAT GCTCCAAAGA CCAATGATTG TAAAGCCAAC TCCAGGAAGA 
CCTGATCATC AGATTCCGCC ACGGAGAGGG CCCCTCAGCC GAGACGGCTC TTTTGGTCCA 
TCCCCTGTGA GTGGTGGTGC ACCCTCCCCA CCACTGATGA TAGAACCGCC TATACGGTCA 
GTCTCTGCTA CTCTTAACCG AAGAGATGTA CCTAGAAGTG AATTTGGATC TATGGATGGG 
CCTCCTCTTG GTCCTAGTCT GAGGAGGTCA TCTGAAGTAT CTGGAAGAAC GTCTGCCTCT 
GATTTGGGTC CTGGTCCAGT CCCTTTGATG AATAGTGGCC CAAGAAGCTC TTCTCCCTCA 
ACAGTGCTGG ATGGCATGCA AACCCTTCCC CCAGAGGCTG AAGGCCCCTG TGCTCCTACT 
GTTCTACCAT CAGCTGAGGA GTCAGTACCA GTTAACACTG GTCCCAAAGG CCACCCACCA 
TTCCCAGGAA TGCCCGTCAT GTCACCAATA GGAGGACCTC CACCACCACC TCTTCGATTT 
GGACCACCTC CTCCACTACG AGGACCTTAT GGGCCTCGAC CTCTTCCTCC ACCTCTTGTT 
CCTGGTGGTC CTCCACCTCC AAGCTTTCGG GAGTACTTAC ATGGCCTTCC ACCAGGAGTA 
AGGGACCTAC CTCCTGACCC TCGAGAGTAT ATACGTGGAC ACCCACCTTT TGGACCTCTA 
GGCCCTCCTG GCCCAAGAGA GTACCTTCCT CCTGGCCCAC GACTGCCGCC TCCAGCCCAT 
GGTCCAAGGG ACTACCCACC TCCACCTTTT ACAAGAGACT TGACACCTTC AGGTTCTAGA 
GACTGTGCCC CCCCTCATCC ACCTCATGCC TCTCAAGGAA GCACACAGGA CTATGCACAG 
GCTCCAGAAC AGAGCCCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020837866.1
CDS	275..6355
Translation	MAAAPRLPFCLLVLLFSTGPRLGQANRSGDRRFAELKRCSDEECSMLMYRGRALKDFTGPDCRFVNFKEGEAVYVYYKLVGRSPELWAGSVGSVFGYFPKDLIEVNHQYSEQELELPTDETDFVCFDGGTDNFDNYDVEDLLGFGGETVRGKGEDELNTSGTESSETPTEEMEEEHPKKVNIEEPLEVSKDGNDEPEIAVDSKVLANSEENSLPPENTKNLEEEFETQRPPVHVNSHADNPQGNQPFLEPLEEILQDKLKVPESENAKNINISQLSKDWEEIDAYKLLKKEINLDLKTKFGSTADALISDDETTRLVTSLEDEFDEELDTDDSRLEEEDNESFDEPPLLTFTNGEDGKTPGNTVVEKYSTGENTEGDPDETAQLRTGEKNDKNILKALGDTILSIVGGGEEERDATDLDGSDLDEEKVEDNEKVLTSKWEEDSTPLTMEVPVTNHDKNLDDALEIGMATEIKENSERIQSSTEEFMKNGMELGDEDIFIKSPTHETDLKLNNPAEKREDTLKSPLNDVGGDLKGTIIHQTTKGEKSGEKVLDDNLESDLVHKTLWKKMKDKNTEQKSINTKPLLEDEQQNASKGNVDIIGISENEQEQEMFPVKMQGKESIELSGLRTQNQVNISSVDQTDLPREKVEENLMLGENLMDQQEKDMKTEISKKLIEKIRHSEREIRDQLSKDGSKEMESTQSKDKDIPQDGETNYFKENVGQPLIIETTEFQTESDDFYTAEDLLEDENAISAAASDQSSRINVDVQMREKATSGVTNLIHTTEDTKEKTSMILETEGVDSSLQESGRTMPKEVTAKVGESIKIAIGQQEKNPFKKDEKPTSSAISDSSGKDENQAQDLVEETNYFEKEDGPKNKKDFSYTERSWPDDFSQEDLEYSQNFTDAESQTNQQSDSTELDDNLVYKKAVKSEYSDTIKQLPIIKGFLDEKRVERLEKYLGSENVLLMESTFHDMESKLELAQKESLAYNMEKALEDVFRSSESNILDMVEKMLDSRVAENKELGMKEIDMFDEEAALLDDIQDLIYFVRYKHPLVEESVPLAAAEPPKQSSDVPLAVFPIEQKTEPKGDQRRRFSVNEGDTLAPEENKRPPESIEIKVHKVEVPEEDMDISEKDDLLDSGEIRDISDSEDRLLVKESSPDQSIVEDLARGMVSVEDTPLDTKQHPLEIISTEETPLDTEQHPLGTVSTEETPLDTEQQLETNPDVPLILAAMKSASLVAKESMKQYTEMLIATLPEDIRPGPDFRGMPWEPIIITVLLGIVSLAIFFWRTFLSVKSRVYQVTEKQLAEKIKTLLQEKTEILEKISEYDQKIKKAKESVKETKKQNINLSDEAAGLKDKMRGLEETNQKLGDKVKNLHSLLETEKEQNVKKQDMILETQKSIEKLQEVIAMHSVELSEVQIALNEAKFSEEKVKSELHHVQEENARLKKRKEQLLQEAEGWSERHTELSEQIKLYQKTQKDTEEALAYKENEIEVLTNCIMQLKQLDLDSESERKNNEEGNGWDDLANGELSDKITSDNRNEKMKSQIKQMMDVSRVKTTLTIVEEDRNQLQSKLSDEIKARHELEEQIKKLEHDSSSLQSAKAQLENECKTLQQKVEILNELYQQKEMVLQKKLTQEEYERQEKEQKLTAADEKVSLVAEEVKTYKQRIQEMEDELQKTERSYKNQIASHEKKAHDNWLIARAAERALAEEKREAANLRQKLIEVNQKIAMLQRPMIVKPTPGRPDHQIPPRRGPLSRDGSFGPSPVSGGAPSPPLMIEPPIRSVSATLNRRDVPRSEFGSMDGPPLGPSLRRSSEVSGRTSASDLGPGPVPLMNSGPRSSSPSTVLDGMQTLPPEAEGPCAPTVLPSAEESVPVNTGPKGHPPFPGMPVMSPIGGPPPPPLRFGPPPPLRGPYGPRPLPPPLVPGGPPPPSFREYLHGLPPGVRDLPPDPREYIRGHPPFGPLGPPGPREYLPPGPRLPPPAHGPRDYPPPPFTRDLTPSGSRDCAPPHPPHASQGSTQDYAQAPEQSP

Target ORF information:

RefSeq Version	XM_020982207.1
Organism	Phascolarctos cinereus(koala)
Definition	Phascolarctos cinereus MIA family member 3, ER export factor (MIA3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020982207.1

ORF Insert Sequence:

ATGGCTGCAG CGCCGCGTCT GCCCTTCTGT CTCCTCGTTC TCCTCTTCTC CACTGGGCCG 
CGGCTGGGTC AAGCGAACCG AAGCGGGGAC CGGCGCTTCG CGGAGCTGAA ACGCTGCTCG 
GACGAGGAGT GCAGCATGTT GATGTACCGA GGTAGAGCTC TGAAGGATTT CACAGGTCCA 
GACTGTCGCT TTGTAAATTT CAAAGAAGGG GAAGCTGTAT ATGTCTATTA CAAATTAGTA 
GGAAGATCAC CTGAACTTTG GGCTGGAAGT GTTGGCAGTG TTTTTGGGTA CTTTCCAAAG 
GATCTAATTG AAGTAAATCA TCAGTATTCT GAACAAGAAT TAGAACTACC AACAGATGAA 
ACAGATTTTG TTTGTTTTGA TGGAGGAACG GATAATTTTG ACAACTATGA TGTTGAAGAT 
CTTTTAGGAT TTGGGGGAGA AACTGTAAGA GGCAAAGGGG AAGATGAATT GAACACTTCA 
GGAACTGAAA GCTCTGAGAC ACCTACAGAA GAAATGGAGG AGGAGCATCC TAAGAAGGTA 
AATATTGAAG AACCCCTTGA AGTATCCAAG GATGGCAATG ATGAGCCTGA AATAGCAGTG 
GACAGTAAAG TTCTTGCAAA TTCGGAAGAA AACAGCTTAC CTCCTGAAAA CACTAAGAAT 
CTAGAGGAAG AATTTGAAAC ACAGAGGCCC CCAGTCCATG TAAATAGTCA TGCAGATAAT 
CCTCAGGGAA ACCAACCTTT CCTTGAGCCC TTAGAAGAAA TACTGCAAGA TAAATTAAAA 
GTGCCAGAGA GTGAGAATGC CAAAAATATC AATATTTCTC AGCTTTCCAA GGATTGGGAG 
GAAATTGACG CCTATAAACT TTTGAAAAAG GAAATTAATT TGGATTTGAA GACAAAATTT 
GGCTCAACTG CAGATGCACT TATATCTGAT GATGAAACAA CTAGGCTTGT TACTTCATTA 
GAAGATGAAT TTGATGAGGA ATTGGATACT GATGATTCCA GACTTGAGGA AGAGGACAAT 
GAAAGCTTTG ATGAACCACC ATTACTAACT TTTACAAATG GAGAAGATGG GAAAACACCA 
GGGAACACAG TAGTTGAGAA ATATTCAACA GGTGAAAATA CTGAAGGAGA CCCTGATGAA 
ACAGCTCAGC TTAGGACGGG TGAAAAGAAT GATAAAAATA TACTGAAAGC CTTAGGAGAT 
ACTATTTTAT CTATTGTTGG TGGAGGTGAA GAAGAAAGAG ATGCCACAGA TTTGGATGGC 
TCTGATTTAG ATGAAGAAAA GGTAGAAGAT AATGAAAAAG TCCTAACTAG TAAATGGGAA 
GAAGACAGCA CTCCTTTGAC TATGGAAGTG CCTGTAACAA ATCATGACAA AAACCTAGAC 
GATGCCCTTG AAATTGGCAT GGCAACTGAA ATTAAGGAAA ATAGTGAGAG AATACAGTCA 
TCCACAGAAG AATTCATGAA GAATGGAATG GAATTAGGAG ATGAAGACAT TTTTATAAAA 
AGTCCTACTC ATGAAACTGA CCTTAAACTT AATAATCCTG CTGAAAAAAG AGAAGACACT 
TTAAAATCAC CCCTTAATGA TGTGGGAGGT GACCTAAAGG GAACCATTAT TCACCAGACC 
ACAAAAGGGG AAAAGTCTGG AGAGAAGGTT TTAGATGACA ACTTAGAGAG TGATCTTGTA 
CATAAAACTC TCTGGAAGAA AATGAAAGAC AAAAATACTG AACAGAAATC TATAAATACT 
AAACCACTGT TAGAAGATGA ACAACAAAAT GCATCCAAAG GAAACGTGGA TATAATAGGT 
ATTTCAGAAA ATGAACAAGA GCAGGAAATG TTTCCAGTGA AGATGCAAGG AAAGGAATCC 
ATAGAGCTAT CTGGGCTTAG AACTCAAAAT CAAGTCAACA TTTCTTCTGT AGATCAAACT 
GATTTACCAA GAGAAAAGGT GGAGGAGAAT CTTATGTTGG GAGAAAATTT AATGGACCAG 
CAGGAAAAAG ATATGAAAAC TGAGATTAGC AAGAAGCTGA TTGAAAAGAT CAGGCATTCT 
GAAAGAGAGA TTCGAGATCA ACTTTCCAAA GATGGAAGCA AAGAAATGGA GAGCACACAA 
AGTAAAGACA AAGATATTCC TCAGGATGGA GAAACAAATT ATTTCAAAGA AAACGTAGGC 
CAGCCCCTTA TCATAGAGAC AACAGAATTC CAGACAGAAA GTGATGATTT CTATACAGCT 
GAGGATTTGT TAGAAGATGA AAATGCTATA AGCGCTGCTG CTAGTGACCA GAGCAGTAGG 
ATAAATGTTG ATGTGCAAAT GAGAGAAAAA GCTACTTCAG GAGTCACTAA TCTGATTCAT 
ACCACGGAAG ACACCAAAGA GAAAACAAGT ATGATTTTGG AAACTGAGGG AGTAGATAGC 
AGCTTGCAAG AGTCAGGCAG GACTATGCCC AAAGAGGTAA CTGCAAAGGT TGGAGAATCT 
ATTAAAATAG CTATAGGGCA GCAAGAAAAG AATCCCTTCA AAAAAGATGA AAAACCCACA 
TCCTCTGCAA TTAGTGACTC TTCTGGCAAG GATGAAAATC AGGCTCAAGA TTTAGTTGAA 
GAGACTAATT ACTTTGAAAA AGAAGATGGT CCAAAAAACA AAAAAGACTT CAGTTACACA 
GAAAGGTCTT GGCCAGATGA TTTCTCCCAA GAGGACCTTG AATATTCACA GAATTTCACT 
GATGCAGAAA GCCAAACAAA TCAGCAATCT GATTCTACAG AGCTTGATGA TAACTTGGTA 
TATAAAAAAG CTGTTAAGTC AGAATATAGT GACACAATAA AGCAACTGCC TATCATTAAA 
GGCTTTCTTG ATGAAAAACG AGTAGAAAGA TTAGAAAAGT ACCTTGGGTC TGAGAATGTC 
CTCTTAATGG AATCCACATT CCATGACATG GAAAGTAAGC TAGAGCTTGC TCAAAAGGAG 
AGTCTGGCAT ATAATATGGA AAAAGCCCTG GAAGATGTCT TTCGCTCTTC AGAATCAAAC 
ATCCTTGATA TGGTAGAGAA AATGTTGGAT TCAAGAGTGG CAGAAAACAA GGAACTGGGC 
ATGAAGGAGA TAGACATGTT TGATGAAGAG GCTGCTCTGC TGGATGATAT TCAGGACCTG 
ATCTATTTTG TCAGATACAA ACACCCTTTG GTAGAAGAGA GTGTACCACT AGCAGCTGCT 
GAGCCTCCAA AACAAAGTTC TGATGTGCCT CTTGCAGTAT TTCCTATAGA ACAAAAAACA 
GAACCCAAAG GAGACCAGAG AAGAAGGTTT TCAGTGAATG AAGGAGATAC ATTGGCACCA 
GAGGAAAACA AGAGGCCACC AGAGAGTATA GAAATAAAAG TTCATAAAGT GGAAGTACCA 
GAGGAGGATA TGGACATCTC AGAAAAGGAT GATCTTTTGG ATTCAGGAGA GATCAGAGAT 
ATCAGTGACT CAGAAGATAG ACTACTGGTT AAAGAGTCCT CCCCAGACCA AAGTATTGTG 
GAAGATTTGG CTAGAGGAAT GGTTTCAGTA GAAGATACTC CTTTGGATAC AAAACAACAT 
CCTCTTGAAA TAATTTCCAC AGAAGAAACT CCTTTAGATA CAGAACAACA TCCTCTAGGT 
ACAGTTTCCA CAGAAGAGAC TCCTTTGGAT ACAGAACAAC AACTAGAAAC AAATCCAGAT 
GTGCCACTGA TTCTTGCAGC TATGAAGAGT GCAAGTCTTG TAGCAAAAGA AAGCATGAAA 
CAATATACTG AGATGTTGAT TGCTACATTA CCTGAGGATA TTCGACCTGG TCCTGATTTT 
CGTGGAATGC CCTGGGAGCC TATAATTATT ACAGTCTTAC TGGGAATTGT TTCACTTGCC 
ATTTTCTTCT GGAGAACGTT TCTTTCAGTG AAGAGTAGAG TTTATCAAGT GACTGAAAAA 
CAGCTTGCTG AGAAGATTAA AACTCTTTTA CAAGAAAAAA CAGAGATTTT AGAAAAAATA 
TCAGAATATG ATCAGAAGAT CAAAAAAGCA AAGGAGTCAG TGAAGGAAAC CAAGAAACAA 
AATATTAATC TTTCTGATGA AGCAGCTGGC CTTAAGGATA AAATGAGAGG GCTTGAAGAA 
ACAAACCAAA AACTGGGTGA CAAAGTGAAA AATCTCCATT CATTGTTGGA AACTGAGAAA 
GAACAGAATG TAAAGAAACA GGACATGATA TTGGAAACTC AGAAATCAAT AGAAAAGCTA 
CAAGAAGTTA TTGCAATGCA TTCTGTTGAA CTTTCAGAGG TGCAGATAGC CCTTAATGAA 
GCTAAGTTCA GTGAAGAGAA AGTAAAGTCT GAACTGCATC ATGTGCAAGA AGAAAATGCA 
CGACTCAAAA AGAGAAAGGA GCAGCTGCTG CAGGAAGCAG AAGGTTGGAG TGAGCGACAC 
ACTGAACTCA GTGAGCAAAT CAAATTATAT CAGAAGACTC AGAAAGACAC CGAAGAAGCC 
CTTGCTTACA AAGAGAATGA AATTGAAGTT TTGACTAATT GCATTATGCA GCTGAAACAA 
CTTGATCTTG ATTCTGAATC TGAGCGCAAA AATAATGAAG AAGGAAATGG ATGGGATGAC 
TTAGCCAATG GAGAACTGTC AGATAAAATC ACCTCAGATA ATCGGAATGA GAAGATGAAG 
AGCCAGATTA AACAGATGAT GGATGTCTCA CGGGTTAAAA CTACATTAAC AATAGTTGAA 
GAAGATAGAA ACCAATTGCA GTCCAAACTC AGTGATGAAA TAAAAGCTCG GCATGAATTG 
GAAGAACAAA TTAAGAAGTT GGAACATGAC TCTTCTTCTC TGCAGTCAGC CAAAGCACAG 
TTGGAAAATG AATGTAAAAC TCTGCAACAA AAAGTGGAGA TTCTTAATGA ACTCTACCAG 
CAGAAAGAGA TGGTTCTGCA GAAGAAGCTC ACTCAGGAAG AGTATGAAAG GCAAGAAAAG 
GAGCAAAAGC TCACTGCTGC AGATGAGAAA GTCAGCTTGG TTGCAGAAGA AGTGAAAACT 
TATAAGCAAA GAATTCAGGA GATGGAGGAT GAATTACAGA AAACAGAGAG GTCATATAAG 
AATCAGATTG CTAGTCATGA GAAGAAGGCC CATGACAACT GGCTCATTGC TCGTGCTGCA 
GAAAGGGCTC TTGCAGAGGA GAAGAGGGAA GCAGCCAACC TGAGACAGAA ATTAATAGAA 
GTGAATCAAA AGATTGCAAT GCTCCAAAGA CCAATGATTG TAAAGCCAAC TCCAGGAAGA 
CCTGATCATC AGATTCCGCC ACGGAGAGGG CCCCTCAGCC GAGACGGCTC TTTTGGTCCA 
TCCCCTGTGA GTGGTGGTGC ACCCTCCCCA CCACTGATGA TAGAACCGCC TATACGGTCA 
GTCTCTGCTA CTCTTAACCG AAGAGATGTA CCTAGAAGTG AATTTGGATC TATGGATGGG 
CCTCCTCTTG GTCCTAGTCT GAGGAGGTCA TCTGAAGTAT CTGGAAGAAC GTCTGCCTCT 
GATTTGGGTC CTGGTCCAGT CCCTTTGATG AATAGTGGCC CAAGAAGCTC TTCTCCCTCA 
ACAGTGCTGG ATGGCATGCA AACCCTTCCC CCAGAGGCTG AAGGCCCCTG TGCTCCTACT 
GTTCTACCAT CAGCTGAGGA GTCAGTACCA GTTAACACTG GTCCCAAAGG CCACCCACCA 
TTCCCAGGAA TGCCCGTCAT GTCACCAATA GGAGGACCTC CACCACCACC TCTTCGATTT 
GGACCACCTC CTCCACTACG AGGACCTTAT GGGCCTCGAC CTCTTCCTCC ACCTCTTGTT 
CCTGGTGGTC CTCCACCTCC AAGCTTTCGG GAGTACTTAC ATGGCCTTCC ACCAGGAGTA 
AGGGACCTAC CTCCTGACCC TCGAGAGTAT ATACGTGGAC ACCCACCTTT TGGACCTCTA 
GGCCCTCCTG GCCCAAGAGA GTACCTTCCT CCTGGCCCAC GACTGCCGCC TCCAGCCCAT 
GGTCCAAGGG ACTACCCACC TCCACCTTTT ACAAGAGACT TGACACCTTC AGGTTCTAGA 
GACTGTGCCC CCCCTCATCC ACCTCATGCC TCTCAAGGAA GCACACAGGA CTATGCACAG 
GCTCCAGAAC AGAGCCCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MIA3 ( XM_020982208.1 ) cDNA ORF clone, Phascolarctos cinereus(koala) -> XP_020837867.1 Phascolarctos cinereus MIA family member 3, ER export factor (MIA3), transcript variant X2, mRNA.

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CloneID	OPh251843
Clone ID Related Accession (Same CDS sequence)	XM_020982207.1 , XM_020982208.1
Accession Version	XM_020982208.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6081bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-09
Organism	Phascolarctos cinereus(koala)
Product	melanoma inhibitory activity protein 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018343964.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Phascolarctos cinereus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCAG CGCCGCGTCT GCCCTTCTGT CTCCTCGTTC TCCTCTTCTC CACTGGGCCG 
CGGCTGGGTC AAGCGAACCG AAGCGGGGAC CGGCGCTTCG CGGAGCTGAA ACGCTGCTCG 
GACGAGGAGT GCAGCATGTT GATGTACCGA GGTAGAGCTC TGAAGGATTT CACAGGTCCA 
GACTGTCGCT TTGTAAATTT CAAAGAAGGG GAAGCTGTAT ATGTCTATTA CAAATTAGTA 
GGAAGATCAC CTGAACTTTG GGCTGGAAGT GTTGGCAGTG TTTTTGGGTA CTTTCCAAAG 
GATCTAATTG AAGTAAATCA TCAGTATTCT GAACAAGAAT TAGAACTACC AACAGATGAA 
ACAGATTTTG TTTGTTTTGA TGGAGGAACG GATAATTTTG ACAACTATGA TGTTGAAGAT 
CTTTTAGGAT TTGGGGGAGA AACTGTAAGA GGCAAAGGGG AAGATGAATT GAACACTTCA 
GGAACTGAAA GCTCTGAGAC ACCTACAGAA GAAATGGAGG AGGAGCATCC TAAGAAGGTA 
AATATTGAAG AACCCCTTGA AGTATCCAAG GATGGCAATG ATGAGCCTGA AATAGCAGTG 
GACAGTAAAG TTCTTGCAAA TTCGGAAGAA AACAGCTTAC CTCCTGAAAA CACTAAGAAT 
CTAGAGGAAG AATTTGAAAC ACAGAGGCCC CCAGTCCATG TAAATAGTCA TGCAGATAAT 
CCTCAGGGAA ACCAACCTTT CCTTGAGCCC TTAGAAGAAA TACTGCAAGA TAAATTAAAA 
GTGCCAGAGA GTGAGAATGC CAAAAATATC AATATTTCTC AGCTTTCCAA GGATTGGGAG 
GAAATTGACG CCTATAAACT TTTGAAAAAG GAAATTAATT TGGATTTGAA GACAAAATTT 
GGCTCAACTG CAGATGCACT TATATCTGAT GATGAAACAA CTAGGCTTGT TACTTCATTA 
GAAGATGAAT TTGATGAGGA ATTGGATACT GATGATTCCA GACTTGAGGA AGAGGACAAT 
GAAAGCTTTG ATGAACCACC ATTACTAACT TTTACAAATG GAGAAGATGG GAAAACACCA 
GGGAACACAG TAGTTGAGAA ATATTCAACA GGTGAAAATA CTGAAGGAGA CCCTGATGAA 
ACAGCTCAGC TTAGGACGGG TGAAAAGAAT GATAAAAATA TACTGAAAGC CTTAGGAGAT 
ACTATTTTAT CTATTGTTGG TGGAGGTGAA GAAGAAAGAG ATGCCACAGA TTTGGATGGC 
TCTGATTTAG ATGAAGAAAA GGTAGAAGAT AATGAAAAAG TCCTAACTAG TAAATGGGAA 
GAAGACAGCA CTCCTTTGAC TATGGAAGTG CCTGTAACAA ATCATGACAA AAACCTAGAC 
GATGCCCTTG AAATTGGCAT GGCAACTGAA ATTAAGGAAA ATAGTGAGAG AATACAGTCA 
TCCACAGAAG AATTCATGAA GAATGGAATG GAATTAGGAG ATGAAGACAT TTTTATAAAA 
AGTCCTACTC ATGAAACTGA CCTTAAACTT AATAATCCTG CTGAAAAAAG AGAAGACACT 
TTAAAATCAC CCCTTAATGA TGTGGGAGGT GACCTAAAGG GAACCATTAT TCACCAGACC 
ACAAAAGGGG AAAAGTCTGG AGAGAAGGTT TTAGATGACA ACTTAGAGAG TGATCTTGTA 
CATAAAACTC TCTGGAAGAA AATGAAAGAC AAAAATACTG AACAGAAATC TATAAATACT 
AAACCACTGT TAGAAGATGA ACAACAAAAT GCATCCAAAG GAAACGTGGA TATAATAGGT 
ATTTCAGAAA ATGAACAAGA GCAGGAAATG TTTCCAGTGA AGATGCAAGG AAAGGAATCC 
ATAGAGCTAT CTGGGCTTAG AACTCAAAAT CAAGTCAACA TTTCTTCTGT AGATCAAACT 
GATTTACCAA GAGAAAAGGT GGAGGAGAAT CTTATGTTGG GAGAAAATTT AATGGACCAG 
CAGGAAAAAG ATATGAAAAC TGAGATTAGC AAGAAGCTGA TTGAAAAGAT CAGGCATTCT 
GAAAGAGAGA TTCGAGATCA ACTTTCCAAA GATGGAAGCA AAGAAATGGA GAGCACACAA 
AGTAAAGACA AAGATATTCC TCAGGATGGA GAAACAAATT ATTTCAAAGA AAACGTAGGC 
CAGCCCCTTA TCATAGAGAC AACAGAATTC CAGACAGAAA GTGATGATTT CTATACAGCT 
GAGGATTTGT TAGAAGATGA AAATGCTATA AGCGCTGCTG CTAGTGACCA GAGCAGTAGG 
ATAAATGTTG ATGTGCAAAT GAGAGAAAAA GCTACTTCAG GAGTCACTAA TCTGATTCAT 
ACCACGGAAG ACACCAAAGA GAAAACAAGT ATGATTTTGG AAACTGAGGG AGTAGATAGC 
AGCTTGCAAG AGTCAGGCAG GACTATGCCC AAAGAGGTAA CTGCAAAGGT TGGAGAATCT 
ATTAAAATAG CTATAGGGCA GCAAGAAAAG AATCCCTTCA AAAAAGATGA AAAACCCACA 
TCCTCTGCAA TTAGTGACTC TTCTGGCAAG GATGAAAATC AGGCTCAAGA TTTAGTTGAA 
GAGACTAATT ACTTTGAAAA AGAAGATGGT CCAAAAAACA AAAAAGACTT CAGTTACACA 
GAAAGGTCTT GGCCAGATGA TTTCTCCCAA GAGGACCTTG AATATTCACA GAATTTCACT 
GATGCAGAAA GCCAAACAAA TCAGCAATCT GATTCTACAG AGCTTGATGA TAACTTGGTA 
TATAAAAAAG CTGTTAAGTC AGAATATAGT GACACAATAA AGCAACTGCC TATCATTAAA 
GGCTTTCTTG ATGAAAAACG AGTAGAAAGA TTAGAAAAGT ACCTTGGGTC TGAGAATGTC 
CTCTTAATGG AATCCACATT CCATGACATG GAAAGTAAGC TAGAGCTTGC TCAAAAGGAG 
AGTCTGGCAT ATAATATGGA AAAAGCCCTG GAAGATGTCT TTCGCTCTTC AGAATCAAAC 
ATCCTTGATA TGGTAGAGAA AATGTTGGAT TCAAGAGTGG CAGAAAACAA GGAACTGGGC 
ATGAAGGAGA TAGACATGTT TGATGAAGAG GCTGCTCTGC TGGATGATAT TCAGGACCTG 
ATCTATTTTG TCAGATACAA ACACCCTTTG GTAGAAGAGA GTGTACCACT AGCAGCTGCT 
GAGCCTCCAA AACAAAGTTC TGATGTGCCT CTTGCAGTAT TTCCTATAGA ACAAAAAACA 
GAACCCAAAG GAGACCAGAG AAGAAGGTTT TCAGTGAATG AAGGAGATAC ATTGGCACCA 
GAGGAAAACA AGAGGCCACC AGAGAGTATA GAAATAAAAG TTCATAAAGT GGAAGTACCA 
GAGGAGGATA TGGACATCTC AGAAAAGGAT GATCTTTTGG ATTCAGGAGA GATCAGAGAT 
ATCAGTGACT CAGAAGATAG ACTACTGGTT AAAGAGTCCT CCCCAGACCA AAGTATTGTG 
GAAGATTTGG CTAGAGGAAT GGTTTCAGTA GAAGATACTC CTTTGGATAC AAAACAACAT 
CCTCTTGAAA TAATTTCCAC AGAAGAAACT CCTTTAGATA CAGAACAACA TCCTCTAGGT 
ACAGTTTCCA CAGAAGAGAC TCCTTTGGAT ACAGAACAAC AACTAGAAAC AAATCCAGAT 
GTGCCACTGA TTCTTGCAGC TATGAAGAGT GCAAGTCTTG TAGCAAAAGA AAGCATGAAA 
CAATATACTG AGATGTTGAT TGCTACATTA CCTGAGGATA TTCGACCTGG TCCTGATTTT 
CGTGGAATGC CCTGGGAGCC TATAATTATT ACAGTCTTAC TGGGAATTGT TTCACTTGCC 
ATTTTCTTCT GGAGAACGTT TCTTTCAGTG AAGAGTAGAG TTTATCAAGT GACTGAAAAA 
CAGCTTGCTG AGAAGATTAA AACTCTTTTA CAAGAAAAAA CAGAGATTTT AGAAAAAATA 
TCAGAATATG ATCAGAAGAT CAAAAAAGCA AAGGAGTCAG TGAAGGAAAC CAAGAAACAA 
AATATTAATC TTTCTGATGA AGCAGCTGGC CTTAAGGATA AAATGAGAGG GCTTGAAGAA 
ACAAACCAAA AACTGGGTGA CAAAGTGAAA AATCTCCATT CATTGTTGGA AACTGAGAAA 
GAACAGAATG TAAAGAAACA GGACATGATA TTGGAAACTC AGAAATCAAT AGAAAAGCTA 
CAAGAAGTTA TTGCAATGCA TTCTGTTGAA CTTTCAGAGG TGCAGATAGC CCTTAATGAA 
GCTAAGTTCA GTGAAGAGAA AGTAAAGTCT GAACTGCATC ATGTGCAAGA AGAAAATGCA 
CGACTCAAAA AGAGAAAGGA GCAGCTGCTG CAGGAAGCAG AAGGTTGGAG TGAGCGACAC 
ACTGAACTCA GTGAGCAAAT CAAATTATAT CAGAAGACTC AGAAAGACAC CGAAGAAGCC 
CTTGCTTACA AAGAGAATGA AATTGAAGTT TTGACTAATT GCATTATGCA GCTGAAACAA 
CTTGATCTTG ATTCTGAATC TGAGCGCAAA AATAATGAAG AAGGAAATGG ATGGGATGAC 
TTAGCCAATG GAGAACTGTC AGATAAAATC ACCTCAGATA ATCGGAATGA GAAGATGAAG 
AGCCAGATTA AACAGATGAT GGATGTCTCA CGGGTTAAAA CTACATTAAC AATAGTTGAA 
GAAGATAGAA ACCAATTGCA GTCCAAACTC AGTGATGAAA TAAAAGCTCG GCATGAATTG 
GAAGAACAAA TTAAGAAGTT GGAACATGAC TCTTCTTCTC TGCAGTCAGC CAAAGCACAG 
TTGGAAAATG AATGTAAAAC TCTGCAACAA AAAGTGGAGA TTCTTAATGA ACTCTACCAG 
CAGAAAGAGA TGGTTCTGCA GAAGAAGCTC ACTCAGGAAG AGTATGAAAG GCAAGAAAAG 
GAGCAAAAGC TCACTGCTGC AGATGAGAAA GTCAGCTTGG TTGCAGAAGA AGTGAAAACT 
TATAAGCAAA GAATTCAGGA GATGGAGGAT GAATTACAGA AAACAGAGAG GTCATATAAG 
AATCAGATTG CTAGTCATGA GAAGAAGGCC CATGACAACT GGCTCATTGC TCGTGCTGCA 
GAAAGGGCTC TTGCAGAGGA GAAGAGGGAA GCAGCCAACC TGAGACAGAA ATTAATAGAA 
GTGAATCAAA AGATTGCAAT GCTCCAAAGA CCAATGATTG TAAAGCCAAC TCCAGGAAGA 
CCTGATCATC AGATTCCGCC ACGGAGAGGG CCCCTCAGCC GAGACGGCTC TTTTGGTCCA 
TCCCCTGTGA GTGGTGGTGC ACCCTCCCCA CCACTGATGA TAGAACCGCC TATACGGTCA 
GTCTCTGCTA CTCTTAACCG AAGAGATGTA CCTAGAAGTG AATTTGGATC TATGGATGGG 
CCTCCTCTTG GTCCTAGTCT GAGGAGGTCA TCTGAAGTAT CTGGAAGAAC GTCTGCCTCT 
GATTTGGGTC CTGGTCCAGT CCCTTTGATG AATAGTGGCC CAAGAAGCTC TTCTCCCTCA 
ACAGTGCTGG ATGGCATGCA AACCCTTCCC CCAGAGGCTG AAGGCCCCTG TGCTCCTACT 
GTTCTACCAT CAGCTGAGGA GTCAGTACCA GTTAACACTG GTCCCAAAGG CCACCCACCA 
TTCCCAGGAA TGCCCGTCAT GTCACCAATA GGAGGACCTC CACCACCACC TCTTCGATTT 
GGACCACCTC CTCCACTACG AGGACCTTAT GGGCCTCGAC CTCTTCCTCC ACCTCTTGTT 
CCTGGTGGTC CTCCACCTCC AAGCTTTCGG GAGTACTTAC ATGGCCTTCC ACCAGGAGTA 
AGGGACCTAC CTCCTGACCC TCGAGAGTAT ATACGTGGAC ACCCACCTTT TGGACCTCTA 
GGCCCTCCTG GCCCAAGAGA GTACCTTCCT CCTGGCCCAC GACTGCCGCC TCCAGCCCAT 
GGTCCAAGGG ACTACCCACC TCCACCTTTT ACAAGAGACT TGACACCTTC AGGTTCTAGA 
GACTGTGCCC CCCCTCATCC ACCTCATGCC TCTCAAGGAA GCACACAGGA CTATGCACAG 
GCTCCAGAAC AGAGCCCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020837867.1
CDS	275..6355
Translation	MAAAPRLPFCLLVLLFSTGPRLGQANRSGDRRFAELKRCSDEECSMLMYRGRALKDFTGPDCRFVNFKEGEAVYVYYKLVGRSPELWAGSVGSVFGYFPKDLIEVNHQYSEQELELPTDETDFVCFDGGTDNFDNYDVEDLLGFGGETVRGKGEDELNTSGTESSETPTEEMEEEHPKKVNIEEPLEVSKDGNDEPEIAVDSKVLANSEENSLPPENTKNLEEEFETQRPPVHVNSHADNPQGNQPFLEPLEEILQDKLKVPESENAKNINISQLSKDWEEIDAYKLLKKEINLDLKTKFGSTADALISDDETTRLVTSLEDEFDEELDTDDSRLEEEDNESFDEPPLLTFTNGEDGKTPGNTVVEKYSTGENTEGDPDETAQLRTGEKNDKNILKALGDTILSIVGGGEEERDATDLDGSDLDEEKVEDNEKVLTSKWEEDSTPLTMEVPVTNHDKNLDDALEIGMATEIKENSERIQSSTEEFMKNGMELGDEDIFIKSPTHETDLKLNNPAEKREDTLKSPLNDVGGDLKGTIIHQTTKGEKSGEKVLDDNLESDLVHKTLWKKMKDKNTEQKSINTKPLLEDEQQNASKGNVDIIGISENEQEQEMFPVKMQGKESIELSGLRTQNQVNISSVDQTDLPREKVEENLMLGENLMDQQEKDMKTEISKKLIEKIRHSEREIRDQLSKDGSKEMESTQSKDKDIPQDGETNYFKENVGQPLIIETTEFQTESDDFYTAEDLLEDENAISAAASDQSSRINVDVQMREKATSGVTNLIHTTEDTKEKTSMILETEGVDSSLQESGRTMPKEVTAKVGESIKIAIGQQEKNPFKKDEKPTSSAISDSSGKDENQAQDLVEETNYFEKEDGPKNKKDFSYTERSWPDDFSQEDLEYSQNFTDAESQTNQQSDSTELDDNLVYKKAVKSEYSDTIKQLPIIKGFLDEKRVERLEKYLGSENVLLMESTFHDMESKLELAQKESLAYNMEKALEDVFRSSESNILDMVEKMLDSRVAENKELGMKEIDMFDEEAALLDDIQDLIYFVRYKHPLVEESVPLAAAEPPKQSSDVPLAVFPIEQKTEPKGDQRRRFSVNEGDTLAPEENKRPPESIEIKVHKVEVPEEDMDISEKDDLLDSGEIRDISDSEDRLLVKESSPDQSIVEDLARGMVSVEDTPLDTKQHPLEIISTEETPLDTEQHPLGTVSTEETPLDTEQQLETNPDVPLILAAMKSASLVAKESMKQYTEMLIATLPEDIRPGPDFRGMPWEPIIITVLLGIVSLAIFFWRTFLSVKSRVYQVTEKQLAEKIKTLLQEKTEILEKISEYDQKIKKAKESVKETKKQNINLSDEAAGLKDKMRGLEETNQKLGDKVKNLHSLLETEKEQNVKKQDMILETQKSIEKLQEVIAMHSVELSEVQIALNEAKFSEEKVKSELHHVQEENARLKKRKEQLLQEAEGWSERHTELSEQIKLYQKTQKDTEEALAYKENEIEVLTNCIMQLKQLDLDSESERKNNEEGNGWDDLANGELSDKITSDNRNEKMKSQIKQMMDVSRVKTTLTIVEEDRNQLQSKLSDEIKARHELEEQIKKLEHDSSSLQSAKAQLENECKTLQQKVEILNELYQQKEMVLQKKLTQEEYERQEKEQKLTAADEKVSLVAEEVKTYKQRIQEMEDELQKTERSYKNQIASHEKKAHDNWLIARAAERALAEEKREAANLRQKLIEVNQKIAMLQRPMIVKPTPGRPDHQIPPRRGPLSRDGSFGPSPVSGGAPSPPLMIEPPIRSVSATLNRRDVPRSEFGSMDGPPLGPSLRRSSEVSGRTSASDLGPGPVPLMNSGPRSSSPSTVLDGMQTLPPEAEGPCAPTVLPSAEESVPVNTGPKGHPPFPGMPVMSPIGGPPPPPLRFGPPPPLRGPYGPRPLPPPLVPGGPPPPSFREYLHGLPPGVRDLPPDPREYIRGHPPFGPLGPPGPREYLPPGPRLPPPAHGPRDYPPPPFTRDLTPSGSRDCAPPHPPHASQGSTQDYAQAPEQSP

Target ORF information:

RefSeq Version	XM_020982208.1
Organism	Phascolarctos cinereus(koala)
Definition	Phascolarctos cinereus MIA family member 3, ER export factor (MIA3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020982208.1

ORF Insert Sequence:

ATGGCTGCAG CGCCGCGTCT GCCCTTCTGT CTCCTCGTTC TCCTCTTCTC CACTGGGCCG 
CGGCTGGGTC AAGCGAACCG AAGCGGGGAC CGGCGCTTCG CGGAGCTGAA ACGCTGCTCG 
GACGAGGAGT GCAGCATGTT GATGTACCGA GGTAGAGCTC TGAAGGATTT CACAGGTCCA 
GACTGTCGCT TTGTAAATTT CAAAGAAGGG GAAGCTGTAT ATGTCTATTA CAAATTAGTA 
GGAAGATCAC CTGAACTTTG GGCTGGAAGT GTTGGCAGTG TTTTTGGGTA CTTTCCAAAG 
GATCTAATTG AAGTAAATCA TCAGTATTCT GAACAAGAAT TAGAACTACC AACAGATGAA 
ACAGATTTTG TTTGTTTTGA TGGAGGAACG GATAATTTTG ACAACTATGA TGTTGAAGAT 
CTTTTAGGAT TTGGGGGAGA AACTGTAAGA GGCAAAGGGG AAGATGAATT GAACACTTCA 
GGAACTGAAA GCTCTGAGAC ACCTACAGAA GAAATGGAGG AGGAGCATCC TAAGAAGGTA 
AATATTGAAG AACCCCTTGA AGTATCCAAG GATGGCAATG ATGAGCCTGA AATAGCAGTG 
GACAGTAAAG TTCTTGCAAA TTCGGAAGAA AACAGCTTAC CTCCTGAAAA CACTAAGAAT 
CTAGAGGAAG AATTTGAAAC ACAGAGGCCC CCAGTCCATG TAAATAGTCA TGCAGATAAT 
CCTCAGGGAA ACCAACCTTT CCTTGAGCCC TTAGAAGAAA TACTGCAAGA TAAATTAAAA 
GTGCCAGAGA GTGAGAATGC CAAAAATATC AATATTTCTC AGCTTTCCAA GGATTGGGAG 
GAAATTGACG CCTATAAACT TTTGAAAAAG GAAATTAATT TGGATTTGAA GACAAAATTT 
GGCTCAACTG CAGATGCACT TATATCTGAT GATGAAACAA CTAGGCTTGT TACTTCATTA 
GAAGATGAAT TTGATGAGGA ATTGGATACT GATGATTCCA GACTTGAGGA AGAGGACAAT 
GAAAGCTTTG ATGAACCACC ATTACTAACT TTTACAAATG GAGAAGATGG GAAAACACCA 
GGGAACACAG TAGTTGAGAA ATATTCAACA GGTGAAAATA CTGAAGGAGA CCCTGATGAA 
ACAGCTCAGC TTAGGACGGG TGAAAAGAAT GATAAAAATA TACTGAAAGC CTTAGGAGAT 
ACTATTTTAT CTATTGTTGG TGGAGGTGAA GAAGAAAGAG ATGCCACAGA TTTGGATGGC 
TCTGATTTAG ATGAAGAAAA GGTAGAAGAT AATGAAAAAG TCCTAACTAG TAAATGGGAA 
GAAGACAGCA CTCCTTTGAC TATGGAAGTG CCTGTAACAA ATCATGACAA AAACCTAGAC 
GATGCCCTTG AAATTGGCAT GGCAACTGAA ATTAAGGAAA ATAGTGAGAG AATACAGTCA 
TCCACAGAAG AATTCATGAA GAATGGAATG GAATTAGGAG ATGAAGACAT TTTTATAAAA 
AGTCCTACTC ATGAAACTGA CCTTAAACTT AATAATCCTG CTGAAAAAAG AGAAGACACT 
TTAAAATCAC CCCTTAATGA TGTGGGAGGT GACCTAAAGG GAACCATTAT TCACCAGACC 
ACAAAAGGGG AAAAGTCTGG AGAGAAGGTT TTAGATGACA ACTTAGAGAG TGATCTTGTA 
CATAAAACTC TCTGGAAGAA AATGAAAGAC AAAAATACTG AACAGAAATC TATAAATACT 
AAACCACTGT TAGAAGATGA ACAACAAAAT GCATCCAAAG GAAACGTGGA TATAATAGGT 
ATTTCAGAAA ATGAACAAGA GCAGGAAATG TTTCCAGTGA AGATGCAAGG AAAGGAATCC 
ATAGAGCTAT CTGGGCTTAG AACTCAAAAT CAAGTCAACA TTTCTTCTGT AGATCAAACT 
GATTTACCAA GAGAAAAGGT GGAGGAGAAT CTTATGTTGG GAGAAAATTT AATGGACCAG 
CAGGAAAAAG ATATGAAAAC TGAGATTAGC AAGAAGCTGA TTGAAAAGAT CAGGCATTCT 
GAAAGAGAGA TTCGAGATCA ACTTTCCAAA GATGGAAGCA AAGAAATGGA GAGCACACAA 
AGTAAAGACA AAGATATTCC TCAGGATGGA GAAACAAATT ATTTCAAAGA AAACGTAGGC 
CAGCCCCTTA TCATAGAGAC AACAGAATTC CAGACAGAAA GTGATGATTT CTATACAGCT 
GAGGATTTGT TAGAAGATGA AAATGCTATA AGCGCTGCTG CTAGTGACCA GAGCAGTAGG 
ATAAATGTTG ATGTGCAAAT GAGAGAAAAA GCTACTTCAG GAGTCACTAA TCTGATTCAT 
ACCACGGAAG ACACCAAAGA GAAAACAAGT ATGATTTTGG AAACTGAGGG AGTAGATAGC 
AGCTTGCAAG AGTCAGGCAG GACTATGCCC AAAGAGGTAA CTGCAAAGGT TGGAGAATCT 
ATTAAAATAG CTATAGGGCA GCAAGAAAAG AATCCCTTCA AAAAAGATGA AAAACCCACA 
TCCTCTGCAA TTAGTGACTC TTCTGGCAAG GATGAAAATC AGGCTCAAGA TTTAGTTGAA 
GAGACTAATT ACTTTGAAAA AGAAGATGGT CCAAAAAACA AAAAAGACTT CAGTTACACA 
GAAAGGTCTT GGCCAGATGA TTTCTCCCAA GAGGACCTTG AATATTCACA GAATTTCACT 
GATGCAGAAA GCCAAACAAA TCAGCAATCT GATTCTACAG AGCTTGATGA TAACTTGGTA 
TATAAAAAAG CTGTTAAGTC AGAATATAGT GACACAATAA AGCAACTGCC TATCATTAAA 
GGCTTTCTTG ATGAAAAACG AGTAGAAAGA TTAGAAAAGT ACCTTGGGTC TGAGAATGTC 
CTCTTAATGG AATCCACATT CCATGACATG GAAAGTAAGC TAGAGCTTGC TCAAAAGGAG 
AGTCTGGCAT ATAATATGGA AAAAGCCCTG GAAGATGTCT TTCGCTCTTC AGAATCAAAC 
ATCCTTGATA TGGTAGAGAA AATGTTGGAT TCAAGAGTGG CAGAAAACAA GGAACTGGGC 
ATGAAGGAGA TAGACATGTT TGATGAAGAG GCTGCTCTGC TGGATGATAT TCAGGACCTG 
ATCTATTTTG TCAGATACAA ACACCCTTTG GTAGAAGAGA GTGTACCACT AGCAGCTGCT 
GAGCCTCCAA AACAAAGTTC TGATGTGCCT CTTGCAGTAT TTCCTATAGA ACAAAAAACA 
GAACCCAAAG GAGACCAGAG AAGAAGGTTT TCAGTGAATG AAGGAGATAC ATTGGCACCA 
GAGGAAAACA AGAGGCCACC AGAGAGTATA GAAATAAAAG TTCATAAAGT GGAAGTACCA 
GAGGAGGATA TGGACATCTC AGAAAAGGAT GATCTTTTGG ATTCAGGAGA GATCAGAGAT 
ATCAGTGACT CAGAAGATAG ACTACTGGTT AAAGAGTCCT CCCCAGACCA AAGTATTGTG 
GAAGATTTGG CTAGAGGAAT GGTTTCAGTA GAAGATACTC CTTTGGATAC AAAACAACAT 
CCTCTTGAAA TAATTTCCAC AGAAGAAACT CCTTTAGATA CAGAACAACA TCCTCTAGGT 
ACAGTTTCCA CAGAAGAGAC TCCTTTGGAT ACAGAACAAC AACTAGAAAC AAATCCAGAT 
GTGCCACTGA TTCTTGCAGC TATGAAGAGT GCAAGTCTTG TAGCAAAAGA AAGCATGAAA 
CAATATACTG AGATGTTGAT TGCTACATTA CCTGAGGATA TTCGACCTGG TCCTGATTTT 
CGTGGAATGC CCTGGGAGCC TATAATTATT ACAGTCTTAC TGGGAATTGT TTCACTTGCC 
ATTTTCTTCT GGAGAACGTT TCTTTCAGTG AAGAGTAGAG TTTATCAAGT GACTGAAAAA 
CAGCTTGCTG AGAAGATTAA AACTCTTTTA CAAGAAAAAA CAGAGATTTT AGAAAAAATA 
TCAGAATATG ATCAGAAGAT CAAAAAAGCA AAGGAGTCAG TGAAGGAAAC CAAGAAACAA 
AATATTAATC TTTCTGATGA AGCAGCTGGC CTTAAGGATA AAATGAGAGG GCTTGAAGAA 
ACAAACCAAA AACTGGGTGA CAAAGTGAAA AATCTCCATT CATTGTTGGA AACTGAGAAA 
GAACAGAATG TAAAGAAACA GGACATGATA TTGGAAACTC AGAAATCAAT AGAAAAGCTA 
CAAGAAGTTA TTGCAATGCA TTCTGTTGAA CTTTCAGAGG TGCAGATAGC CCTTAATGAA 
GCTAAGTTCA GTGAAGAGAA AGTAAAGTCT GAACTGCATC ATGTGCAAGA AGAAAATGCA 
CGACTCAAAA AGAGAAAGGA GCAGCTGCTG CAGGAAGCAG AAGGTTGGAG TGAGCGACAC 
ACTGAACTCA GTGAGCAAAT CAAATTATAT CAGAAGACTC AGAAAGACAC CGAAGAAGCC 
CTTGCTTACA AAGAGAATGA AATTGAAGTT TTGACTAATT GCATTATGCA GCTGAAACAA 
CTTGATCTTG ATTCTGAATC TGAGCGCAAA AATAATGAAG AAGGAAATGG ATGGGATGAC 
TTAGCCAATG GAGAACTGTC AGATAAAATC ACCTCAGATA ATCGGAATGA GAAGATGAAG 
AGCCAGATTA AACAGATGAT GGATGTCTCA CGGGTTAAAA CTACATTAAC AATAGTTGAA 
GAAGATAGAA ACCAATTGCA GTCCAAACTC AGTGATGAAA TAAAAGCTCG GCATGAATTG 
GAAGAACAAA TTAAGAAGTT GGAACATGAC TCTTCTTCTC TGCAGTCAGC CAAAGCACAG 
TTGGAAAATG AATGTAAAAC TCTGCAACAA AAAGTGGAGA TTCTTAATGA ACTCTACCAG 
CAGAAAGAGA TGGTTCTGCA GAAGAAGCTC ACTCAGGAAG AGTATGAAAG GCAAGAAAAG 
GAGCAAAAGC TCACTGCTGC AGATGAGAAA GTCAGCTTGG TTGCAGAAGA AGTGAAAACT 
TATAAGCAAA GAATTCAGGA GATGGAGGAT GAATTACAGA AAACAGAGAG GTCATATAAG 
AATCAGATTG CTAGTCATGA GAAGAAGGCC CATGACAACT GGCTCATTGC TCGTGCTGCA 
GAAAGGGCTC TTGCAGAGGA GAAGAGGGAA GCAGCCAACC TGAGACAGAA ATTAATAGAA 
GTGAATCAAA AGATTGCAAT GCTCCAAAGA CCAATGATTG TAAAGCCAAC TCCAGGAAGA 
CCTGATCATC AGATTCCGCC ACGGAGAGGG CCCCTCAGCC GAGACGGCTC TTTTGGTCCA 
TCCCCTGTGA GTGGTGGTGC ACCCTCCCCA CCACTGATGA TAGAACCGCC TATACGGTCA 
GTCTCTGCTA CTCTTAACCG AAGAGATGTA CCTAGAAGTG AATTTGGATC TATGGATGGG 
CCTCCTCTTG GTCCTAGTCT GAGGAGGTCA TCTGAAGTAT CTGGAAGAAC GTCTGCCTCT 
GATTTGGGTC CTGGTCCAGT CCCTTTGATG AATAGTGGCC CAAGAAGCTC TTCTCCCTCA 
ACAGTGCTGG ATGGCATGCA AACCCTTCCC CCAGAGGCTG AAGGCCCCTG TGCTCCTACT 
GTTCTACCAT CAGCTGAGGA GTCAGTACCA GTTAACACTG GTCCCAAAGG CCACCCACCA 
TTCCCAGGAA TGCCCGTCAT GTCACCAATA GGAGGACCTC CACCACCACC TCTTCGATTT 
GGACCACCTC CTCCACTACG AGGACCTTAT GGGCCTCGAC CTCTTCCTCC ACCTCTTGTT 
CCTGGTGGTC CTCCACCTCC AAGCTTTCGG GAGTACTTAC ATGGCCTTCC ACCAGGAGTA 
AGGGACCTAC CTCCTGACCC TCGAGAGTAT ATACGTGGAC ACCCACCTTT TGGACCTCTA 
GGCCCTCCTG GCCCAAGAGA GTACCTTCCT CCTGGCCCAC GACTGCCGCC TCCAGCCCAT 
GGTCCAAGGG ACTACCCACC TCCACCTTTT ACAAGAGACT TGACACCTTC AGGTTCTAGA 
GACTGTGCCC CCCCTCATCC ACCTCATGCC TCTCAAGGAA GCACACAGGA CTATGCACAG 
GCTCCAGAAC AGAGCCCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MIA3 ( XM_020982209.1 ) cDNA ORF clone, Phascolarctos cinereus(koala) -> XP_020837868.1 Phascolarctos cinereus MIA family member 3, ER export factor (MIA3), transcript variant X3, mRNA.

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CloneID	OPh251844
Clone ID Related Accession (Same CDS sequence)	XM_020982209.1
Accession Version	XM_020982209.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6069bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-09
Organism	Phascolarctos cinereus(koala)
Product	melanoma inhibitory activity protein 3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018343964.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Phascolarctos cinereus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCAG CGCCGCGTCT GCCCTTCTGT CTCCTCGTTC TCCTCTTCTC CACTGGGCCG 
CGGCTGGGTC AAGCGAACCG AAGCGGGGAC CGGCGCTTCG CGGAGCTGAA ACGCTGCTCG 
GACGAGGAGT GCAGCATGTT GATGTACCGA GGTAGAGCTC TGAAGGATTT CACAGGTCCA 
GACTGTCGCT TTGTAAATTT CAAAGAAGGG GAAGCTGTAT ATGTCTATTA CAAATTAGTA 
GGAAGATCAC CTGAACTTTG GGCTGGAAGT GTTGGCAGTG TTTTTGGGTA CTTTCCAAAG 
GATCTAATTG AAGTAAATCA TCAGTATTCT GAACAAGAAT TAGAACTACC AACAGATGAA 
ACAGATTTTG TTTGTTTTGA TGGAGGAACG GATAATTTTG ACAACTATGA TGTTGAAGAT 
CTTTTAGGAT TTGGGGGAGA AACTGTAAGA GGCAAAGGGG AAGATGAATT GAACACTTCA 
GGAACTGAAA GCTCTGAGAC ACCTACAGAA GAAATGGAGG AGGAGCATCC TAAGAAGGTA 
AATATTGAAG AACCCCTTGA AGTATCCAAG GATGGCAATG ATGAGCCTGA AATAGCAGTG 
GACAGTAAAG TTCTTGCAAA TTCGGAAGAA AACAGCTTAC CTCCTGAAAA CACTAAGAAT 
CTAGAGGAAG AATTTGAAAC ACAGAGGCCC CCAGTCCATG TAAATAGTCA TGCAGATAAT 
CCTCAGGGAA ACCAACCTTT CCTTGAGCCC TTAGAAGAAA TACTGCAAGA TAAATTAAAA 
GTGCCAGAGA GTGAGAATGC CAAAAATATC AATATTTCTC AGCTTTCCAA GGATTGGGAG 
GAAATTGACG CCTATAAACT TTTGAAAAAG GAAATTAATT TGGATTTGAA GACAAAATTT 
GGCTCAACTG CAGATGCACT TATATCTGAT GATGAAACAA CTAGGCTTGT TACTTCATTA 
GAAGATGAAT TTGATGAGGA ATTGGATACT GATGATTCCA GACTTGAGGA AGAGGACAAT 
GAAAGCTTTG ATGAACCACC ATTACTAACT TTTACAAATG GAGAAGATGG GAAAACACCA 
GGGAACACAG TAGTTGAGAA ATATTCAACA GGTGAAAATA CTGAAGGAGA CCCTGATGAA 
ACAGCTCAGC TTAGGACGGG TGAAAAGAAT GATAAAAATA TACTGAAAGC CTTAGGAGAT 
ACTATTTTAT CTATTGTTGG TGGAGGTGAA GAAGAAAGAG ATGCCACAGA TTTGGATGGC 
TCTGATTTAG ATGAAGAAAA GGTAGAAGAT AATGAAAAAG TCCTAACTAG TAAATGGGAA 
GAAGACAGCA CTCCTTTGAC TATGGAAGTG CCTGTAACAA ATCATGACAA AAACCTAGAC 
GATGCCCTTG AAATTGGCAT GGCAACTGAA ATTAAGGAAA ATAGTGAGAG AATACAGTCA 
TCCACAGAAG AATTCATGAA GAATGGAATG GAATTAGGAG ATGAAGACAT TTTTATAAAA 
AGTCCTACTC ATGAAACTGA CCTTAAACTT AATAATCCTG CTGAAAAAAG AGAAGACACT 
TTAAAATCAC CCCTTAATGA TGTGGGAGGT GACCTAAAGG GAACCATTAT TCACCAGACC 
ACAAAAGGGG AAAAGTCTGG AGAGAAGGTT TTAGATGACA ACTTAGAGAG TGATCTTGTA 
CATAAAACTC TCTGGAAGAA AATGAAAGAC AAAAATACTG AACAGAAATC TATAAATACT 
AAACCACTGT TAGAAGATGA ACAACAAAAT GCATCCAAAG GAAACGTGGA TATAATAGGT 
ATTTCAGAAA ATGAACAAGA GCAGGAAATG TTTCCAGTGA AGATGCAAGG AAAGGAATCC 
ATAGAGCTAT CTGGGCTTAG AACTCAAAAT CAAGTCAACA TTTCTTCTGT AGATCAAACT 
GATTTACCAA GAGAAAAGGT GGAGGAGAAT CTTATGTTGG GAGAAAATTT AATGGACCAG 
CAGGAAAAAG ATATGAAAAC TGAGATTAGC AAGAAGCTGA TTGAAAAGAT CAGGCATTCT 
GAAAGAGAGA TTCGAGATCA ACTTTCCAAA GATGGAAGCA AAGAAATGGA GAGCACACAA 
AGTAAAGACA AAGATATTCC TCAGGATGGA GAAACAAATT ATTTCAAAGA AAACGTAGGC 
CAGCCCCTTA TCATAGAGAC AACAGAATTC CAGACAGAAA GTGATGATTT CTATACAGCT 
GAGGATTTGT TAGAAGATGA AAATGCTATA AGCGCTGCTG CTAGTGACCA GAGCAGTAGG 
ATAAATGTTG ATGTGCAAAT GAGAGAAAAA GCTACTTCAG GAGTCACTAA TCTGATTCAT 
ACCACGGAAG ACACCAAAGA GAAAACAAGT ATGATTTTGG AAACTGAGGG AGTAGATAGC 
AGCTTGCAAG AGTCAGGCAG GACTATGCCC AAAGAGGTAA CTGCAAAGGT TGGAGAATCT 
ATTAAAATAG CTATAGGGCA GCAAGAAAAG AATCCCTTCA AAAAAGATGA AAAACCCACA 
TCCTCTGCAA TTAGTGACTC TTCTGGCAAG GATGAAAATC AGGCTCAAGA TTTAGTTGAA 
GAGACTAATT ACTTTGAAAA AGAAGATGGT CCAAAAAACA AAAAAGACTT CAGTTACACA 
GAAAGGTCTT GGCCAGATGA TTTCTCCCAA GAGGACCTTG AATATTCACA GAATTTCACT 
GATGCAGAAA GCCAAACAAA TCAGCAATCT GATTCTACAG AGCTTGATGA TAACTTGGTA 
TATAAAAAAG CTGTTAAGTC AGAATATAGT GACACAATAA AGCAACTGCC TATCATTAAA 
GGCTTTCTTG ATGAAAAACG AGTAGAAAGA TTAGAAAAGT ACCTTGGGTC TGAGAATGTC 
CTCTTAATGG AATCCACATT CCATGACATG GAAAGTAAGC TAGAGCTTGC TCAAAAGGAG 
AGTCTGGCAT ATAATATGGA AAAAGCCCTG GAAGATGTCT TTCGCTCTTC AGAATCAAAC 
ATCCTTGATA TGGTAGAGAA AATGTTGGAT TCAAGAGTGG CAGAAAACAA GGAACTGGGC 
ATGAAGGAGA TAGACATGTT TGATGAAGAG GCTGCTCTGC TGGATGATAT TCAGGACCTG 
ATCTATTTTG TCAGATACAA ACACCCTTTG GTAGAAGAGA GTGTACCACT AGCAGCTGCT 
GAGCCTCCAA AACAAAGTTC TGATGTGCCT CTTGCAGAAC AAAAAACAGA ACCCAAAGGA 
GACCAGAGAA GAAGGTTTTC AGTGAATGAA GGAGATACAT TGGCACCAGA GGAAAACAAG 
AGGCCACCAG AGAGTATAGA AATAAAAGTT CATAAAGTGG AAGTACCAGA GGAGGATATG 
GACATCTCAG AAAAGGATGA TCTTTTGGAT TCAGGAGAGA TCAGAGATAT CAGTGACTCA 
GAAGATAGAC TACTGGTTAA AGAGTCCTCC CCAGACCAAA GTATTGTGGA AGATTTGGCT 
AGAGGAATGG TTTCAGTAGA AGATACTCCT TTGGATACAA AACAACATCC TCTTGAAATA 
ATTTCCACAG AAGAAACTCC TTTAGATACA GAACAACATC CTCTAGGTAC AGTTTCCACA 
GAAGAGACTC CTTTGGATAC AGAACAACAA CTAGAAACAA ATCCAGATGT GCCACTGATT 
CTTGCAGCTA TGAAGAGTGC AAGTCTTGTA GCAAAAGAAA GCATGAAACA ATATACTGAG 
ATGTTGATTG CTACATTACC TGAGGATATT CGACCTGGTC CTGATTTTCG TGGAATGCCC 
TGGGAGCCTA TAATTATTAC AGTCTTACTG GGAATTGTTT CACTTGCCAT TTTCTTCTGG 
AGAACGTTTC TTTCAGTGAA GAGTAGAGTT TATCAAGTGA CTGAAAAACA GCTTGCTGAG 
AAGATTAAAA CTCTTTTACA AGAAAAAACA GAGATTTTAG AAAAAATATC AGAATATGAT 
CAGAAGATCA AAAAAGCAAA GGAGTCAGTG AAGGAAACCA AGAAACAAAA TATTAATCTT 
TCTGATGAAG CAGCTGGCCT TAAGGATAAA ATGAGAGGGC TTGAAGAAAC AAACCAAAAA 
CTGGGTGACA AAGTGAAAAA TCTCCATTCA TTGTTGGAAA CTGAGAAAGA ACAGAATGTA 
AAGAAACAGG ACATGATATT GGAAACTCAG AAATCAATAG AAAAGCTACA AGAAGTTATT 
GCAATGCATT CTGTTGAACT TTCAGAGGTG CAGATAGCCC TTAATGAAGC TAAGTTCAGT 
GAAGAGAAAG TAAAGTCTGA ACTGCATCAT GTGCAAGAAG AAAATGCACG ACTCAAAAAG 
AGAAAGGAGC AGCTGCTGCA GGAAGCAGAA GGTTGGAGTG AGCGACACAC TGAACTCAGT 
GAGCAAATCA AATTATATCA GAAGACTCAG AAAGACACCG AAGAAGCCCT TGCTTACAAA 
GAGAATGAAA TTGAAGTTTT GACTAATTGC ATTATGCAGC TGAAACAACT TGATCTTGAT 
TCTGAATCTG AGCGCAAAAA TAATGAAGAA GGAAATGGAT GGGATGACTT AGCCAATGGA 
GAACTGTCAG ATAAAATCAC CTCAGATAAT CGGAATGAGA AGATGAAGAG CCAGATTAAA 
CAGATGATGG ATGTCTCACG GGTTAAAACT ACATTAACAA TAGTTGAAGA AGATAGAAAC 
CAATTGCAGT CCAAACTCAG TGATGAAATA AAAGCTCGGC ATGAATTGGA AGAACAAATT 
AAGAAGTTGG AACATGACTC TTCTTCTCTG CAGTCAGCCA AAGCACAGTT GGAAAATGAA 
TGTAAAACTC TGCAACAAAA AGTGGAGATT CTTAATGAAC TCTACCAGCA GAAAGAGATG 
GTTCTGCAGA AGAAGCTCAC TCAGGAAGAG TATGAAAGGC AAGAAAAGGA GCAAAAGCTC 
ACTGCTGCAG ATGAGAAAGT CAGCTTGGTT GCAGAAGAAG TGAAAACTTA TAAGCAAAGA 
ATTCAGGAGA TGGAGGATGA ATTACAGAAA ACAGAGAGGT CATATAAGAA TCAGATTGCT 
AGTCATGAGA AGAAGGCCCA TGACAACTGG CTCATTGCTC GTGCTGCAGA AAGGGCTCTT 
GCAGAGGAGA AGAGGGAAGC AGCCAACCTG AGACAGAAAT TAATAGAAGT GAATCAAAAG 
ATTGCAATGC TCCAAAGACC AATGATTGTA AAGCCAACTC CAGGAAGACC TGATCATCAG 
ATTCCGCCAC GGAGAGGGCC CCTCAGCCGA GACGGCTCTT TTGGTCCATC CCCTGTGAGT 
GGTGGTGCAC CCTCCCCACC ACTGATGATA GAACCGCCTA TACGGTCAGT CTCTGCTACT 
CTTAACCGAA GAGATGTACC TAGAAGTGAA TTTGGATCTA TGGATGGGCC TCCTCTTGGT 
CCTAGTCTGA GGAGGTCATC TGAAGTATCT GGAAGAACGT CTGCCTCTGA TTTGGGTCCT 
GGTCCAGTCC CTTTGATGAA TAGTGGCCCA AGAAGCTCTT CTCCCTCAAC AGTGCTGGAT 
GGCATGCAAA CCCTTCCCCC AGAGGCTGAA GGCCCCTGTG CTCCTACTGT TCTACCATCA 
GCTGAGGAGT CAGTACCAGT TAACACTGGT CCCAAAGGCC ACCCACCATT CCCAGGAATG 
CCCGTCATGT CACCAATAGG AGGACCTCCA CCACCACCTC TTCGATTTGG ACCACCTCCT 
CCACTACGAG GACCTTATGG GCCTCGACCT CTTCCTCCAC CTCTTGTTCC TGGTGGTCCT 
CCACCTCCAA GCTTTCGGGA GTACTTACAT GGCCTTCCAC CAGGAGTAAG GGACCTACCT 
CCTGACCCTC GAGAGTATAT ACGTGGACAC CCACCTTTTG GACCTCTAGG CCCTCCTGGC 
CCAAGAGAGT ACCTTCCTCC TGGCCCACGA CTGCCGCCTC CAGCCCATGG TCCAAGGGAC 
TACCCACCTC CACCTTTTAC AAGAGACTTG ACACCTTCAG GTTCTAGAGA CTGTGCCCCC 
CCTCATCCAC CTCATGCCTC TCAAGGAAGC ACACAGGACT ATGCACAGGC TCCAGAACAG 
AGCCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020837868.1
CDS	275..6343
Translation	MAAAPRLPFCLLVLLFSTGPRLGQANRSGDRRFAELKRCSDEECSMLMYRGRALKDFTGPDCRFVNFKEGEAVYVYYKLVGRSPELWAGSVGSVFGYFPKDLIEVNHQYSEQELELPTDETDFVCFDGGTDNFDNYDVEDLLGFGGETVRGKGEDELNTSGTESSETPTEEMEEEHPKKVNIEEPLEVSKDGNDEPEIAVDSKVLANSEENSLPPENTKNLEEEFETQRPPVHVNSHADNPQGNQPFLEPLEEILQDKLKVPESENAKNINISQLSKDWEEIDAYKLLKKEINLDLKTKFGSTADALISDDETTRLVTSLEDEFDEELDTDDSRLEEEDNESFDEPPLLTFTNGEDGKTPGNTVVEKYSTGENTEGDPDETAQLRTGEKNDKNILKALGDTILSIVGGGEEERDATDLDGSDLDEEKVEDNEKVLTSKWEEDSTPLTMEVPVTNHDKNLDDALEIGMATEIKENSERIQSSTEEFMKNGMELGDEDIFIKSPTHETDLKLNNPAEKREDTLKSPLNDVGGDLKGTIIHQTTKGEKSGEKVLDDNLESDLVHKTLWKKMKDKNTEQKSINTKPLLEDEQQNASKGNVDIIGISENEQEQEMFPVKMQGKESIELSGLRTQNQVNISSVDQTDLPREKVEENLMLGENLMDQQEKDMKTEISKKLIEKIRHSEREIRDQLSKDGSKEMESTQSKDKDIPQDGETNYFKENVGQPLIIETTEFQTESDDFYTAEDLLEDENAISAAASDQSSRINVDVQMREKATSGVTNLIHTTEDTKEKTSMILETEGVDSSLQESGRTMPKEVTAKVGESIKIAIGQQEKNPFKKDEKPTSSAISDSSGKDENQAQDLVEETNYFEKEDGPKNKKDFSYTERSWPDDFSQEDLEYSQNFTDAESQTNQQSDSTELDDNLVYKKAVKSEYSDTIKQLPIIKGFLDEKRVERLEKYLGSENVLLMESTFHDMESKLELAQKESLAYNMEKALEDVFRSSESNILDMVEKMLDSRVAENKELGMKEIDMFDEEAALLDDIQDLIYFVRYKHPLVEESVPLAAAEPPKQSSDVPLAEQKTEPKGDQRRRFSVNEGDTLAPEENKRPPESIEIKVHKVEVPEEDMDISEKDDLLDSGEIRDISDSEDRLLVKESSPDQSIVEDLARGMVSVEDTPLDTKQHPLEIISTEETPLDTEQHPLGTVSTEETPLDTEQQLETNPDVPLILAAMKSASLVAKESMKQYTEMLIATLPEDIRPGPDFRGMPWEPIIITVLLGIVSLAIFFWRTFLSVKSRVYQVTEKQLAEKIKTLLQEKTEILEKISEYDQKIKKAKESVKETKKQNINLSDEAAGLKDKMRGLEETNQKLGDKVKNLHSLLETEKEQNVKKQDMILETQKSIEKLQEVIAMHSVELSEVQIALNEAKFSEEKVKSELHHVQEENARLKKRKEQLLQEAEGWSERHTELSEQIKLYQKTQKDTEEALAYKENEIEVLTNCIMQLKQLDLDSESERKNNEEGNGWDDLANGELSDKITSDNRNEKMKSQIKQMMDVSRVKTTLTIVEEDRNQLQSKLSDEIKARHELEEQIKKLEHDSSSLQSAKAQLENECKTLQQKVEILNELYQQKEMVLQKKLTQEEYERQEKEQKLTAADEKVSLVAEEVKTYKQRIQEMEDELQKTERSYKNQIASHEKKAHDNWLIARAAERALAEEKREAANLRQKLIEVNQKIAMLQRPMIVKPTPGRPDHQIPPRRGPLSRDGSFGPSPVSGGAPSPPLMIEPPIRSVSATLNRRDVPRSEFGSMDGPPLGPSLRRSSEVSGRTSASDLGPGPVPLMNSGPRSSSPSTVLDGMQTLPPEAEGPCAPTVLPSAEESVPVNTGPKGHPPFPGMPVMSPIGGPPPPPLRFGPPPPLRGPYGPRPLPPPLVPGGPPPPSFREYLHGLPPGVRDLPPDPREYIRGHPPFGPLGPPGPREYLPPGPRLPPPAHGPRDYPPPPFTRDLTPSGSRDCAPPHPPHASQGSTQDYAQAPEQSP

Target ORF information:

RefSeq Version	XM_020982209.1
Organism	Phascolarctos cinereus(koala)
Definition	Phascolarctos cinereus MIA family member 3, ER export factor (MIA3), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020982209.1

ORF Insert Sequence:

ATGGCTGCAG CGCCGCGTCT GCCCTTCTGT CTCCTCGTTC TCCTCTTCTC CACTGGGCCG 
CGGCTGGGTC AAGCGAACCG AAGCGGGGAC CGGCGCTTCG CGGAGCTGAA ACGCTGCTCG 
GACGAGGAGT GCAGCATGTT GATGTACCGA GGTAGAGCTC TGAAGGATTT CACAGGTCCA 
GACTGTCGCT TTGTAAATTT CAAAGAAGGG GAAGCTGTAT ATGTCTATTA CAAATTAGTA 
GGAAGATCAC CTGAACTTTG GGCTGGAAGT GTTGGCAGTG TTTTTGGGTA CTTTCCAAAG 
GATCTAATTG AAGTAAATCA TCAGTATTCT GAACAAGAAT TAGAACTACC AACAGATGAA 
ACAGATTTTG TTTGTTTTGA TGGAGGAACG GATAATTTTG ACAACTATGA TGTTGAAGAT 
CTTTTAGGAT TTGGGGGAGA AACTGTAAGA GGCAAAGGGG AAGATGAATT GAACACTTCA 
GGAACTGAAA GCTCTGAGAC ACCTACAGAA GAAATGGAGG AGGAGCATCC TAAGAAGGTA 
AATATTGAAG AACCCCTTGA AGTATCCAAG GATGGCAATG ATGAGCCTGA AATAGCAGTG 
GACAGTAAAG TTCTTGCAAA TTCGGAAGAA AACAGCTTAC CTCCTGAAAA CACTAAGAAT 
CTAGAGGAAG AATTTGAAAC ACAGAGGCCC CCAGTCCATG TAAATAGTCA TGCAGATAAT 
CCTCAGGGAA ACCAACCTTT CCTTGAGCCC TTAGAAGAAA TACTGCAAGA TAAATTAAAA 
GTGCCAGAGA GTGAGAATGC CAAAAATATC AATATTTCTC AGCTTTCCAA GGATTGGGAG 
GAAATTGACG CCTATAAACT TTTGAAAAAG GAAATTAATT TGGATTTGAA GACAAAATTT 
GGCTCAACTG CAGATGCACT TATATCTGAT GATGAAACAA CTAGGCTTGT TACTTCATTA 
GAAGATGAAT TTGATGAGGA ATTGGATACT GATGATTCCA GACTTGAGGA AGAGGACAAT 
GAAAGCTTTG ATGAACCACC ATTACTAACT TTTACAAATG GAGAAGATGG GAAAACACCA 
GGGAACACAG TAGTTGAGAA ATATTCAACA GGTGAAAATA CTGAAGGAGA CCCTGATGAA 
ACAGCTCAGC TTAGGACGGG TGAAAAGAAT GATAAAAATA TACTGAAAGC CTTAGGAGAT 
ACTATTTTAT CTATTGTTGG TGGAGGTGAA GAAGAAAGAG ATGCCACAGA TTTGGATGGC 
TCTGATTTAG ATGAAGAAAA GGTAGAAGAT AATGAAAAAG TCCTAACTAG TAAATGGGAA 
GAAGACAGCA CTCCTTTGAC TATGGAAGTG CCTGTAACAA ATCATGACAA AAACCTAGAC 
GATGCCCTTG AAATTGGCAT GGCAACTGAA ATTAAGGAAA ATAGTGAGAG AATACAGTCA 
TCCACAGAAG AATTCATGAA GAATGGAATG GAATTAGGAG ATGAAGACAT TTTTATAAAA 
AGTCCTACTC ATGAAACTGA CCTTAAACTT AATAATCCTG CTGAAAAAAG AGAAGACACT 
TTAAAATCAC CCCTTAATGA TGTGGGAGGT GACCTAAAGG GAACCATTAT TCACCAGACC 
ACAAAAGGGG AAAAGTCTGG AGAGAAGGTT TTAGATGACA ACTTAGAGAG TGATCTTGTA 
CATAAAACTC TCTGGAAGAA AATGAAAGAC AAAAATACTG AACAGAAATC TATAAATACT 
AAACCACTGT TAGAAGATGA ACAACAAAAT GCATCCAAAG GAAACGTGGA TATAATAGGT 
ATTTCAGAAA ATGAACAAGA GCAGGAAATG TTTCCAGTGA AGATGCAAGG AAAGGAATCC 
ATAGAGCTAT CTGGGCTTAG AACTCAAAAT CAAGTCAACA TTTCTTCTGT AGATCAAACT 
GATTTACCAA GAGAAAAGGT GGAGGAGAAT CTTATGTTGG GAGAAAATTT AATGGACCAG 
CAGGAAAAAG ATATGAAAAC TGAGATTAGC AAGAAGCTGA TTGAAAAGAT CAGGCATTCT 
GAAAGAGAGA TTCGAGATCA ACTTTCCAAA GATGGAAGCA AAGAAATGGA GAGCACACAA 
AGTAAAGACA AAGATATTCC TCAGGATGGA GAAACAAATT ATTTCAAAGA AAACGTAGGC 
CAGCCCCTTA TCATAGAGAC AACAGAATTC CAGACAGAAA GTGATGATTT CTATACAGCT 
GAGGATTTGT TAGAAGATGA AAATGCTATA AGCGCTGCTG CTAGTGACCA GAGCAGTAGG 
ATAAATGTTG ATGTGCAAAT GAGAGAAAAA GCTACTTCAG GAGTCACTAA TCTGATTCAT 
ACCACGGAAG ACACCAAAGA GAAAACAAGT ATGATTTTGG AAACTGAGGG AGTAGATAGC 
AGCTTGCAAG AGTCAGGCAG GACTATGCCC AAAGAGGTAA CTGCAAAGGT TGGAGAATCT 
ATTAAAATAG CTATAGGGCA GCAAGAAAAG AATCCCTTCA AAAAAGATGA AAAACCCACA 
TCCTCTGCAA TTAGTGACTC TTCTGGCAAG GATGAAAATC AGGCTCAAGA TTTAGTTGAA 
GAGACTAATT ACTTTGAAAA AGAAGATGGT CCAAAAAACA AAAAAGACTT CAGTTACACA 
GAAAGGTCTT GGCCAGATGA TTTCTCCCAA GAGGACCTTG AATATTCACA GAATTTCACT 
GATGCAGAAA GCCAAACAAA TCAGCAATCT GATTCTACAG AGCTTGATGA TAACTTGGTA 
TATAAAAAAG CTGTTAAGTC AGAATATAGT GACACAATAA AGCAACTGCC TATCATTAAA 
GGCTTTCTTG ATGAAAAACG AGTAGAAAGA TTAGAAAAGT ACCTTGGGTC TGAGAATGTC 
CTCTTAATGG AATCCACATT CCATGACATG GAAAGTAAGC TAGAGCTTGC TCAAAAGGAG 
AGTCTGGCAT ATAATATGGA AAAAGCCCTG GAAGATGTCT TTCGCTCTTC AGAATCAAAC 
ATCCTTGATA TGGTAGAGAA AATGTTGGAT TCAAGAGTGG CAGAAAACAA GGAACTGGGC 
ATGAAGGAGA TAGACATGTT TGATGAAGAG GCTGCTCTGC TGGATGATAT TCAGGACCTG 
ATCTATTTTG TCAGATACAA ACACCCTTTG GTAGAAGAGA GTGTACCACT AGCAGCTGCT 
GAGCCTCCAA AACAAAGTTC TGATGTGCCT CTTGCAGAAC AAAAAACAGA ACCCAAAGGA 
GACCAGAGAA GAAGGTTTTC AGTGAATGAA GGAGATACAT TGGCACCAGA GGAAAACAAG 
AGGCCACCAG AGAGTATAGA AATAAAAGTT CATAAAGTGG AAGTACCAGA GGAGGATATG 
GACATCTCAG AAAAGGATGA TCTTTTGGAT TCAGGAGAGA TCAGAGATAT CAGTGACTCA 
GAAGATAGAC TACTGGTTAA AGAGTCCTCC CCAGACCAAA GTATTGTGGA AGATTTGGCT 
AGAGGAATGG TTTCAGTAGA AGATACTCCT TTGGATACAA AACAACATCC TCTTGAAATA 
ATTTCCACAG AAGAAACTCC TTTAGATACA GAACAACATC CTCTAGGTAC AGTTTCCACA 
GAAGAGACTC CTTTGGATAC AGAACAACAA CTAGAAACAA ATCCAGATGT GCCACTGATT 
CTTGCAGCTA TGAAGAGTGC AAGTCTTGTA GCAAAAGAAA GCATGAAACA ATATACTGAG 
ATGTTGATTG CTACATTACC TGAGGATATT CGACCTGGTC CTGATTTTCG TGGAATGCCC 
TGGGAGCCTA TAATTATTAC AGTCTTACTG GGAATTGTTT CACTTGCCAT TTTCTTCTGG 
AGAACGTTTC TTTCAGTGAA GAGTAGAGTT TATCAAGTGA CTGAAAAACA GCTTGCTGAG 
AAGATTAAAA CTCTTTTACA AGAAAAAACA GAGATTTTAG AAAAAATATC AGAATATGAT 
CAGAAGATCA AAAAAGCAAA GGAGTCAGTG AAGGAAACCA AGAAACAAAA TATTAATCTT 
TCTGATGAAG CAGCTGGCCT TAAGGATAAA ATGAGAGGGC TTGAAGAAAC AAACCAAAAA 
CTGGGTGACA AAGTGAAAAA TCTCCATTCA TTGTTGGAAA CTGAGAAAGA ACAGAATGTA 
AAGAAACAGG ACATGATATT GGAAACTCAG AAATCAATAG AAAAGCTACA AGAAGTTATT 
GCAATGCATT CTGTTGAACT TTCAGAGGTG CAGATAGCCC TTAATGAAGC TAAGTTCAGT 
GAAGAGAAAG TAAAGTCTGA ACTGCATCAT GTGCAAGAAG AAAATGCACG ACTCAAAAAG 
AGAAAGGAGC AGCTGCTGCA GGAAGCAGAA GGTTGGAGTG AGCGACACAC TGAACTCAGT 
GAGCAAATCA AATTATATCA GAAGACTCAG AAAGACACCG AAGAAGCCCT TGCTTACAAA 
GAGAATGAAA TTGAAGTTTT GACTAATTGC ATTATGCAGC TGAAACAACT TGATCTTGAT 
TCTGAATCTG AGCGCAAAAA TAATGAAGAA GGAAATGGAT GGGATGACTT AGCCAATGGA 
GAACTGTCAG ATAAAATCAC CTCAGATAAT CGGAATGAGA AGATGAAGAG CCAGATTAAA 
CAGATGATGG ATGTCTCACG GGTTAAAACT ACATTAACAA TAGTTGAAGA AGATAGAAAC 
CAATTGCAGT CCAAACTCAG TGATGAAATA AAAGCTCGGC ATGAATTGGA AGAACAAATT 
AAGAAGTTGG AACATGACTC TTCTTCTCTG CAGTCAGCCA AAGCACAGTT GGAAAATGAA 
TGTAAAACTC TGCAACAAAA AGTGGAGATT CTTAATGAAC TCTACCAGCA GAAAGAGATG 
GTTCTGCAGA AGAAGCTCAC TCAGGAAGAG TATGAAAGGC AAGAAAAGGA GCAAAAGCTC 
ACTGCTGCAG ATGAGAAAGT CAGCTTGGTT GCAGAAGAAG TGAAAACTTA TAAGCAAAGA 
ATTCAGGAGA TGGAGGATGA ATTACAGAAA ACAGAGAGGT CATATAAGAA TCAGATTGCT 
AGTCATGAGA AGAAGGCCCA TGACAACTGG CTCATTGCTC GTGCTGCAGA AAGGGCTCTT 
GCAGAGGAGA AGAGGGAAGC AGCCAACCTG AGACAGAAAT TAATAGAAGT GAATCAAAAG 
ATTGCAATGC TCCAAAGACC AATGATTGTA AAGCCAACTC CAGGAAGACC TGATCATCAG 
ATTCCGCCAC GGAGAGGGCC CCTCAGCCGA GACGGCTCTT TTGGTCCATC CCCTGTGAGT 
GGTGGTGCAC CCTCCCCACC ACTGATGATA GAACCGCCTA TACGGTCAGT CTCTGCTACT 
CTTAACCGAA GAGATGTACC TAGAAGTGAA TTTGGATCTA TGGATGGGCC TCCTCTTGGT 
CCTAGTCTGA GGAGGTCATC TGAAGTATCT GGAAGAACGT CTGCCTCTGA TTTGGGTCCT 
GGTCCAGTCC CTTTGATGAA TAGTGGCCCA AGAAGCTCTT CTCCCTCAAC AGTGCTGGAT 
GGCATGCAAA CCCTTCCCCC AGAGGCTGAA GGCCCCTGTG CTCCTACTGT TCTACCATCA 
GCTGAGGAGT CAGTACCAGT TAACACTGGT CCCAAAGGCC ACCCACCATT CCCAGGAATG 
CCCGTCATGT CACCAATAGG AGGACCTCCA CCACCACCTC TTCGATTTGG ACCACCTCCT 
CCACTACGAG GACCTTATGG GCCTCGACCT CTTCCTCCAC CTCTTGTTCC TGGTGGTCCT 
CCACCTCCAA GCTTTCGGGA GTACTTACAT GGCCTTCCAC CAGGAGTAAG GGACCTACCT 
CCTGACCCTC GAGAGTATAT ACGTGGACAC CCACCTTTTG GACCTCTAGG CCCTCCTGGC 
CCAAGAGAGT ACCTTCCTCC TGGCCCACGA CTGCCGCCTC CAGCCCATGG TCCAAGGGAC 
TACCCACCTC CACCTTTTAC AAGAGACTTG ACACCTTCAG GTTCTAGAGA CTGTGCCCCC 
CCTCATCCAC CTCATGCCTC TCAAGGAAGC ACACAGGACT ATGCACAGGC TCCAGAACAG 
AGCCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MIA3 ( XM_020982211.1 ) cDNA ORF clone, Phascolarctos cinereus(koala) -> XP_020837870.1 Phascolarctos cinereus MIA family member 3, ER export factor (MIA3), transcript variant X4, mRNA.

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CloneID	OPh251845
Clone ID Related Accession (Same CDS sequence)	XM_020982211.1
Accession Version	XM_020982211.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6009bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-09
Organism	Phascolarctos cinereus(koala)
Product	melanoma inhibitory activity protein 3 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018343964.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Phascolarctos cinereus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCAG CGCCGCGTCT GCCCTTCTGT CTCCTCGTTC TCCTCTTCTC CACTGGGCCG 
CGGCTGGGTC AAGCGAACCG AAGCGGGGAC CGGCGCTTCG CGGAGCTGAA ACGCTGCTCG 
GACGAGGAGT GCAGCATGTT GATGTACCGA GGTAGAGCTC TGAAGGATTT CACAGGTCCA 
GACTGTCGCT TTGTAAATTT CAAAGAAGGG GAAGCTGTAT ATGTCTATTA CAAATTAGTA 
GGAAGATCAC CTGAACTTTG GGCTGGAAGT GTTGGCAGTG TTTTTGGGTA CTTTCCAAAG 
GATCTAATTG AAGTAAATCA TCAGTATTCT GAACAAGAAT TAGAACTACC AACAGATGAA 
ACAGATTTTG TTTGTTTTGA TGGAGGAACG GATAATTTTG ACAACTATGA TGTTGAAGAT 
CTTTTAGGAT TTGGGGGAGA AACTGTAAGA GGCAAAGGGG AAGATGAATT GAACACTTCA 
GGAACTGAAA GCTCTGAGAC ACCTACAGAA GAAATGGAGG AGGAGCATCC TAAGAAGGTA 
AATATTGAAG AACCCCTTGA AGTATCCAAG GATGGCAATG ATGAGCCTGA AATAGCAGTG 
GACAGTAAAG TTCTTGCAAA TTCGGAAGAA AACAGCTTAC CTCCTGAAAA CACTAAGAAT 
CTAGAGGAAG AATTTGAAAC ACAGAGGCCC CCAGTCCATG TAAATAGTCA TGCAGATAAT 
CCTCAGGGAA ACCAACCTTT CCTTGAGCCC TTAGAAGAAA TACTGCAAGA TAAATTAAAA 
GTGCCAGAGA GTGAGAATGC CAAAAATATC AATATTTCTC AGCTTTCCAA GGATTGGGAG 
GAAATTGACG CCTATAAACT TTTGAAAAAG GAAATTAATT TGGATTTGAA GACAAAATTT 
GGCTCAACTG CAGATGCACT TATATCTGAT GATGAAACAA CTAGGCTTGT TACTTCATTA 
GAAGATGAAT TTGATGAGGA ATTGGATACT GATGATTCCA GACTTGAGGA AGAGGACAAT 
GAAAGCTTTG ATGAACCACC ATTACTAACT TTTACAAATG GAGAAGATGG GAAAACACCA 
GGGAACACAG TAGTTGAGAA ATATTCAACA GGTGAAAATA CTGAAGGAGA CCCTGATGAA 
ACAGCTCAGC TTAGGACGGG TGAAAAGAAT GATAAAAATA TACTGAAAGC CTTAGGAGAT 
ACTATTTTAT CTATTGTTGG TGGAGGTGAA GAAGAAAGAG ATGCCACAGA TTTGGATGGC 
TCTGATTTAG ATGAAGAAAA GGTAGAAGAT AATGAAAAAG TCCTAACTAG TAAATGGGAA 
GAAGACAGCA CTCCTTTGAC TATGGAAGTG CCTGTAACAA ATCATGACAA AAACCTAGAC 
GATGCCCTTG AAATTGGCAT GGCAACTGAA ATTAAGGAAA ATAGTGAGAG AATACAGTCA 
TCCACAGAAG AATTCATGAA GAATGGAATG GAATTAGGAG ATGAAGACAT TTTTATAAAA 
AGTCCTACTC ATGAAACTGA CCTTAAACTT AATAATCCTG CTGAAAAAAG AGAAGACACT 
TTAAAATCAC CCCTTAATGA TGTGGGAGGT GACCTAAAGG GAACCATTAT TCACCAGACC 
ACAAAAGGGG AAAAGTCTGG AGAGAAGGTT TTAGATGACA ACTTAGAGAG TGATCTTGTA 
CATAAAACTC TCTGGAAGAA AATGAAAGAC AAAAATACTG AACAGAAATC TATAAATACT 
AAACCACTGT TAGAAGATGA ACAACAAAAT GCATCCAAAG GAAACGTGGA TATAATAGGT 
ATTTCAGAAA ATGAACAAGA GCAGGAAATG TTTCCAGTGA AGATGCAAGG AAAGGAATCC 
ATAGAGCTAT CTGGGCTTAG AACTCAAAAT CAAGTCAACA TTTCTTCTGT AGATCAAACT 
GATTTACCAA GAGAAAAGGT GGAGGAGAAT CTTATGTTGG GAGAAAATTT AATGGACCAG 
CAGGAAAAAG ATATGAAAAC TGAGATTAGC AAGAAGCTGA TTGAAAAGAT CAGGCATTCT 
GAAAGAGAGA TTCGAGATCA ACTTTCCAAA GATGGAAGCA AAGAAATGGA GAGCACACAA 
AGTAAAGACA AAGATATTCC TCAGGATGGA GAAACAAATT ATTTCAAAGA AAACGTAGGC 
CAGCCCCTTA TCATAGAGAC AACAGAATTC CAGACAGAAA GTGATGATTT CTATACAGCT 
GAGGATTTGT TAGAAGATGA AAATGCTATA AGCGCTGCTG CTAGTGACCA GAGCAGTAGG 
ATAAATGTTG ATGTGCAAAT GAGAGAAAAA GCTACTTCAG GAGTCACTAA TCTGATTCAT 
ACCACGGAAG ACACCAAAGA GAAAACAAGT ATGATTTTGG AAACTGAGGG AGTAGATAGC 
AGCTTGCAAG AGTCAGGCAG GACTATGCCC AAAGAGGTAA CTGCAAAGGT TGGAGAATCT 
ATTAAAATAG CTATAGGGCA GCAAGAAAAG AATCCCTTCA AAAAAGATGA AAAACCCACA 
TCCTCTGCAA TTAGTGACTC TTCTGGCAAG GATGAAAATC AGGCTCAAGA TTTAGTTGAA 
GAGACTAATT ACTTTGAAAA AGAAGATGGT CCAAAAAACA AAAAAGACTT CAGTTACACA 
GAAAGGTCTT GGCCAGATGA TTTCTCCCAA GAGGACCTTG AATATTCACA GAATTTCACT 
GATGCAGAAA GCCAAACAAA TCAGCAATCT GATTCTACAG AGCTTGATGA TAACTTGGTA 
TATAAAAAAG CTGTTAAGTC AGAATATAGT GACACAATAA AGCAACTGCC TATCATTAAA 
GGCTTTCTTG ATGAAAAACG AGTAGAAAGA TTAGAAAAGT ACCTTGGGTC TGAGAATGTC 
CTCTTAATGG AATCCACATT CCATGACATG GAAAGTAAGC TAGAGCTTGC TCAAAAGGAG 
AGTCTGGCAT ATAATATGGA AAAAGCCCTG GAAGATGTCT TTCGCTCTTC AGAATCAAAC 
ATCCTTGATA TGGTAGAGAA AATGTTGGAT TCAAGAGTGG CAGAAAACAA GGAACTGGGC 
ATGAAGGAGA TAGACATGTT TGATGAAGAG GCTGCTCTGC TGGATGATAT TCAGGACCTG 
ATCTATTTTG TCAGATACAA ACACCCTTTG GTAGAAGAGA GTGTACCACT AGCAGCTGCT 
GAGCCTCCAA AACAAAGTTC TGATGTGCCT CTTGCAGTAT TTCCTATAGA ACAAAAAACA 
GAACCCAAAG GAGACCAGAG AAGAAGGTTT TCAGTGAATG AAGGAGATAC ATTGGCACCA 
GAGGAAAACA AGAGGCCACC AGAGAGTATA GAAATAAAAG TTCATAAAGT GGAAGTACCA 
GAGGAGGATA TGGACATCTC AGAAAAGGAT GATCTTTTGG ATTCAGGAGA GATCAGAGAT 
ATCAGTGACT CAGAAGATAG ACTACTGGTT AAAGAGTCCT CCCCAGACCA AAGTATTGTG 
GAAGATTTGG CTAGAGGAAT GGTTTCAGTA GAAGATACTC CTTTGGATAC AAAACAACAT 
CCTCTTGAAA TAATTTCCAC AGAAGAAACT CCTTTAGATA CAGAACAACA TCCTCTAGGT 
ACAGTTTCCA CAGAAGAGAC TCCTTTGGAT ACAGAACAAC AACTAGAAAC AAATCCAGAT 
GTGCCACTGA TTCTTGCAGC TATGAAGAGT GCAAGTCTTG TAGCAAAAGA AAGCATGAAA 
CAATATACTG AGATGTTGAT TGCTACATTA CCTGAGGATA TTCGACCTGG TCCTGATTTT 
CGTGGAATGC CCTGGGAGCC TATAATTATT ACAGTCTTAC TGGGAATTGT TTCACTTGCC 
ATTTTCTTCT GGAGAACGTT TCTTTCAGTG AAGAGTAGAG TTTATCAAGT GACTGAAAAA 
CAGCTTGCTG AGAAGATTAA AACTCTTTTA CAAGAAAAAA CAGAGATTTT AGAAAAAATA 
TCAGAATATG ATCAGAAGAT CAAAAAAGCA AAGGAGTCAG TGAAGGAAAC CAAGAAACAA 
AATATTAATC TTTCTGATGA AGCAGCTGGC CTTAAGGATA AAATGAGAGG GCTTGAAGAA 
ACAAACCAAA AACTGGGTGA CAAAGTGAAA AATCTCCATT CATTGTTGGA AACTGAGAAA 
GAACAGAATG TAAAGAAACA GGACATGATA TTGGAAACTC AGAAATCAAT AGAAAAGCTA 
CAAGAAGTTA TTGCAATGCA TTCTGTTGAA CTTTCAGAGG TGCAGATAGC CCTTAATGAA 
GCTAAGTTCA GTGAAGAGAA AGTAAAGTCT GAACTGCATC ATGTGCAAGA AGAAAATGCA 
CGACTCAAAA AGAGAAAGGA GCAGCTGCTG CAGGAAGCAG AAGGTTGGAG TGAGCGACAC 
ACTGAACTCA GTGAGCAAAT CAAATTATAT CAGAAGACTC AGAAAGACAC CGAAGAAGCC 
CTTGCTTACA AAGAGAATGA AATTGAAGTT TTGACTAATT GCATTATGCA GCTGAAACAA 
CTTGATCTTG ATTCTGAATC TGAGCGCAAA AATAATGAAG AAGGAAATGG ATGGGATGAC 
TTAGCCAATG GAGAACTGTC AGATAAAATC ACCTCAGATA ATCGGAATGA GAAGATGAAG 
AGCCAGATTA AACAGATGAT GGATGTCTCA CGGGTTAAAA CTACATTAAC AATAGTTGAA 
GAAGATAGAA ACCAATTGCA GTCCAAACTC AGTGATGAAA TAAAAGCTCG GCATGAATTG 
GAAGAACAAA TTAAGAAGTT GGAACATGAC TCTTCTTCTC TGCAGTCAGC CAAAGCACAG 
TTGGAAAATG AATGTAAAAC TCTGCAACAA AAAGTGGAGA TTCTTAATGA ACTCTACCAG 
CAGAAAGAGA TGGTTCTGCA GAAGAAGCTC ACTCAGGAAG AGTATGAAAG GCAAGAAAAG 
GAGCAAAAGC TCACTGCTGC AGATGAGAAA GTCAGCTTGG TTGCAGAAGA AGTGAAAACT 
TATAAGCAAA GAATTCAGGA GATGGAGGAT GAATTACAGA AAACAGAGAG GTCATATAAG 
AATCAGATTG CTAGTCATGA GAAGAAGGCC CATGACAACT GGCTCATTGC TCGTGCTGCA 
GAAAGGGCTC TTGCAGAGGA GAAGAGGGAA GCAGCCAACC TGAGACAGAA ATTAATAGAA 
GTGAATCAAA AGATTGCAAT GCTCCAAAGA CCAATGATTG TAAAGCCAAC TCCAGGAAGA 
CCTGATCATC AGATTCCGCC ACGGAGAGGG CCCCTCAGCC GAGACGGCTC TTTTGGTCCA 
TCCCCTGTGA GTGGTGGTGC ACCCTCCCCA CCACTGATGA TAGAACCGCC TATACGGTCA 
GTCTCTGCTA CTCTTAACCG AAGAGATGTA CCTAGAAGTG AATTTGGATC TATGGATGGG 
CCTCCTCTTG GTCCTAGTCT GAGGAGGTCA TCTGAAGTAT CTGGAAGAAC GTCTGCCTCT 
GATTTGGGTC CTGGTCCAGT CCCTTTGATG AATAGTGGCC CAAGAAGCTC TTCTCCCTCA 
ACAGTGCTGG ATGGCATGGT TAACACTGGT CCCAAAGGCC ACCCACCATT CCCAGGAATG 
CCCGTCATGT CACCAATAGG AGGACCTCCA CCACCACCTC TTCGATTTGG ACCACCTCCT 
CCACTACGAG GACCTTATGG GCCTCGACCT CTTCCTCCAC CTCTTGTTCC TGGTGGTCCT 
CCACCTCCAA GCTTTCGGGA GTACTTACAT GGCCTTCCAC CAGGAGTAAG GGACCTACCT 
CCTGACCCTC GAGAGTATAT ACGTGGACAC CCACCTTTTG GACCTCTAGG CCCTCCTGGC 
CCAAGAGAGT ACCTTCCTCC TGGCCCACGA CTGCCGCCTC CAGCCCATGG TCCAAGGGAC 
TACCCACCTC CACCTTTTAC AAGAGACTTG ACACCTTCAG GTTCTAGAGA CTGTGCCCCC 
CCTCATCCAC CTCATGCCTC TCAAGGAAGC ACACAGGACT ATGCACAGGC TCCAGAACAG 
AGCCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020837870.1
CDS	274..6282
Translation	MAAAPRLPFCLLVLLFSTGPRLGQANRSGDRRFAELKRCSDEECSMLMYRGRALKDFTGPDCRFVNFKEGEAVYVYYKLVGRSPELWAGSVGSVFGYFPKDLIEVNHQYSEQELELPTDETDFVCFDGGTDNFDNYDVEDLLGFGGETVRGKGEDELNTSGTESSETPTEEMEEEHPKKVNIEEPLEVSKDGNDEPEIAVDSKVLANSEENSLPPENTKNLEEEFETQRPPVHVNSHADNPQGNQPFLEPLEEILQDKLKVPESENAKNINISQLSKDWEEIDAYKLLKKEINLDLKTKFGSTADALISDDETTRLVTSLEDEFDEELDTDDSRLEEEDNESFDEPPLLTFTNGEDGKTPGNTVVEKYSTGENTEGDPDETAQLRTGEKNDKNILKALGDTILSIVGGGEEERDATDLDGSDLDEEKVEDNEKVLTSKWEEDSTPLTMEVPVTNHDKNLDDALEIGMATEIKENSERIQSSTEEFMKNGMELGDEDIFIKSPTHETDLKLNNPAEKREDTLKSPLNDVGGDLKGTIIHQTTKGEKSGEKVLDDNLESDLVHKTLWKKMKDKNTEQKSINTKPLLEDEQQNASKGNVDIIGISENEQEQEMFPVKMQGKESIELSGLRTQNQVNISSVDQTDLPREKVEENLMLGENLMDQQEKDMKTEISKKLIEKIRHSEREIRDQLSKDGSKEMESTQSKDKDIPQDGETNYFKENVGQPLIIETTEFQTESDDFYTAEDLLEDENAISAAASDQSSRINVDVQMREKATSGVTNLIHTTEDTKEKTSMILETEGVDSSLQESGRTMPKEVTAKVGESIKIAIGQQEKNPFKKDEKPTSSAISDSSGKDENQAQDLVEETNYFEKEDGPKNKKDFSYTERSWPDDFSQEDLEYSQNFTDAESQTNQQSDSTELDDNLVYKKAVKSEYSDTIKQLPIIKGFLDEKRVERLEKYLGSENVLLMESTFHDMESKLELAQKESLAYNMEKALEDVFRSSESNILDMVEKMLDSRVAENKELGMKEIDMFDEEAALLDDIQDLIYFVRYKHPLVEESVPLAAAEPPKQSSDVPLAVFPIEQKTEPKGDQRRRFSVNEGDTLAPEENKRPPESIEIKVHKVEVPEEDMDISEKDDLLDSGEIRDISDSEDRLLVKESSPDQSIVEDLARGMVSVEDTPLDTKQHPLEIISTEETPLDTEQHPLGTVSTEETPLDTEQQLETNPDVPLILAAMKSASLVAKESMKQYTEMLIATLPEDIRPGPDFRGMPWEPIIITVLLGIVSLAIFFWRTFLSVKSRVYQVTEKQLAEKIKTLLQEKTEILEKISEYDQKIKKAKESVKETKKQNINLSDEAAGLKDKMRGLEETNQKLGDKVKNLHSLLETEKEQNVKKQDMILETQKSIEKLQEVIAMHSVELSEVQIALNEAKFSEEKVKSELHHVQEENARLKKRKEQLLQEAEGWSERHTELSEQIKLYQKTQKDTEEALAYKENEIEVLTNCIMQLKQLDLDSESERKNNEEGNGWDDLANGELSDKITSDNRNEKMKSQIKQMMDVSRVKTTLTIVEEDRNQLQSKLSDEIKARHELEEQIKKLEHDSSSLQSAKAQLENECKTLQQKVEILNELYQQKEMVLQKKLTQEEYERQEKEQKLTAADEKVSLVAEEVKTYKQRIQEMEDELQKTERSYKNQIASHEKKAHDNWLIARAAERALAEEKREAANLRQKLIEVNQKIAMLQRPMIVKPTPGRPDHQIPPRRGPLSRDGSFGPSPVSGGAPSPPLMIEPPIRSVSATLNRRDVPRSEFGSMDGPPLGPSLRRSSEVSGRTSASDLGPGPVPLMNSGPRSSSPSTVLDGMVNTGPKGHPPFPGMPVMSPIGGPPPPPLRFGPPPPLRGPYGPRPLPPPLVPGGPPPPSFREYLHGLPPGVRDLPPDPREYIRGHPPFGPLGPPGPREYLPPGPRLPPPAHGPRDYPPPPFTRDLTPSGSRDCAPPHPPHASQGSTQDYAQAPEQSP

Target ORF information:

RefSeq Version	XM_020982211.1
Organism	Phascolarctos cinereus(koala)
Definition	Phascolarctos cinereus MIA family member 3, ER export factor (MIA3), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020982211.1

ORF Insert Sequence:

ATGGCTGCAG CGCCGCGTCT GCCCTTCTGT CTCCTCGTTC TCCTCTTCTC CACTGGGCCG 
CGGCTGGGTC AAGCGAACCG AAGCGGGGAC CGGCGCTTCG CGGAGCTGAA ACGCTGCTCG 
GACGAGGAGT GCAGCATGTT GATGTACCGA GGTAGAGCTC TGAAGGATTT CACAGGTCCA 
GACTGTCGCT TTGTAAATTT CAAAGAAGGG GAAGCTGTAT ATGTCTATTA CAAATTAGTA 
GGAAGATCAC CTGAACTTTG GGCTGGAAGT GTTGGCAGTG TTTTTGGGTA CTTTCCAAAG 
GATCTAATTG AAGTAAATCA TCAGTATTCT GAACAAGAAT TAGAACTACC AACAGATGAA 
ACAGATTTTG TTTGTTTTGA TGGAGGAACG GATAATTTTG ACAACTATGA TGTTGAAGAT 
CTTTTAGGAT TTGGGGGAGA AACTGTAAGA GGCAAAGGGG AAGATGAATT GAACACTTCA 
GGAACTGAAA GCTCTGAGAC ACCTACAGAA GAAATGGAGG AGGAGCATCC TAAGAAGGTA 
AATATTGAAG AACCCCTTGA AGTATCCAAG GATGGCAATG ATGAGCCTGA AATAGCAGTG 
GACAGTAAAG TTCTTGCAAA TTCGGAAGAA AACAGCTTAC CTCCTGAAAA CACTAAGAAT 
CTAGAGGAAG AATTTGAAAC ACAGAGGCCC CCAGTCCATG TAAATAGTCA TGCAGATAAT 
CCTCAGGGAA ACCAACCTTT CCTTGAGCCC TTAGAAGAAA TACTGCAAGA TAAATTAAAA 
GTGCCAGAGA GTGAGAATGC CAAAAATATC AATATTTCTC AGCTTTCCAA GGATTGGGAG 
GAAATTGACG CCTATAAACT TTTGAAAAAG GAAATTAATT TGGATTTGAA GACAAAATTT 
GGCTCAACTG CAGATGCACT TATATCTGAT GATGAAACAA CTAGGCTTGT TACTTCATTA 
GAAGATGAAT TTGATGAGGA ATTGGATACT GATGATTCCA GACTTGAGGA AGAGGACAAT 
GAAAGCTTTG ATGAACCACC ATTACTAACT TTTACAAATG GAGAAGATGG GAAAACACCA 
GGGAACACAG TAGTTGAGAA ATATTCAACA GGTGAAAATA CTGAAGGAGA CCCTGATGAA 
ACAGCTCAGC TTAGGACGGG TGAAAAGAAT GATAAAAATA TACTGAAAGC CTTAGGAGAT 
ACTATTTTAT CTATTGTTGG TGGAGGTGAA GAAGAAAGAG ATGCCACAGA TTTGGATGGC 
TCTGATTTAG ATGAAGAAAA GGTAGAAGAT AATGAAAAAG TCCTAACTAG TAAATGGGAA 
GAAGACAGCA CTCCTTTGAC TATGGAAGTG CCTGTAACAA ATCATGACAA AAACCTAGAC 
GATGCCCTTG AAATTGGCAT GGCAACTGAA ATTAAGGAAA ATAGTGAGAG AATACAGTCA 
TCCACAGAAG AATTCATGAA GAATGGAATG GAATTAGGAG ATGAAGACAT TTTTATAAAA 
AGTCCTACTC ATGAAACTGA CCTTAAACTT AATAATCCTG CTGAAAAAAG AGAAGACACT 
TTAAAATCAC CCCTTAATGA TGTGGGAGGT GACCTAAAGG GAACCATTAT TCACCAGACC 
ACAAAAGGGG AAAAGTCTGG AGAGAAGGTT TTAGATGACA ACTTAGAGAG TGATCTTGTA 
CATAAAACTC TCTGGAAGAA AATGAAAGAC AAAAATACTG AACAGAAATC TATAAATACT 
AAACCACTGT TAGAAGATGA ACAACAAAAT GCATCCAAAG GAAACGTGGA TATAATAGGT 
ATTTCAGAAA ATGAACAAGA GCAGGAAATG TTTCCAGTGA AGATGCAAGG AAAGGAATCC 
ATAGAGCTAT CTGGGCTTAG AACTCAAAAT CAAGTCAACA TTTCTTCTGT AGATCAAACT 
GATTTACCAA GAGAAAAGGT GGAGGAGAAT CTTATGTTGG GAGAAAATTT AATGGACCAG 
CAGGAAAAAG ATATGAAAAC TGAGATTAGC AAGAAGCTGA TTGAAAAGAT CAGGCATTCT 
GAAAGAGAGA TTCGAGATCA ACTTTCCAAA GATGGAAGCA AAGAAATGGA GAGCACACAA 
AGTAAAGACA AAGATATTCC TCAGGATGGA GAAACAAATT ATTTCAAAGA AAACGTAGGC 
CAGCCCCTTA TCATAGAGAC AACAGAATTC CAGACAGAAA GTGATGATTT CTATACAGCT 
GAGGATTTGT TAGAAGATGA AAATGCTATA AGCGCTGCTG CTAGTGACCA GAGCAGTAGG 
ATAAATGTTG ATGTGCAAAT GAGAGAAAAA GCTACTTCAG GAGTCACTAA TCTGATTCAT 
ACCACGGAAG ACACCAAAGA GAAAACAAGT ATGATTTTGG AAACTGAGGG AGTAGATAGC 
AGCTTGCAAG AGTCAGGCAG GACTATGCCC AAAGAGGTAA CTGCAAAGGT TGGAGAATCT 
ATTAAAATAG CTATAGGGCA GCAAGAAAAG AATCCCTTCA AAAAAGATGA AAAACCCACA 
TCCTCTGCAA TTAGTGACTC TTCTGGCAAG GATGAAAATC AGGCTCAAGA TTTAGTTGAA 
GAGACTAATT ACTTTGAAAA AGAAGATGGT CCAAAAAACA AAAAAGACTT CAGTTACACA 
GAAAGGTCTT GGCCAGATGA TTTCTCCCAA GAGGACCTTG AATATTCACA GAATTTCACT 
GATGCAGAAA GCCAAACAAA TCAGCAATCT GATTCTACAG AGCTTGATGA TAACTTGGTA 
TATAAAAAAG CTGTTAAGTC AGAATATAGT GACACAATAA AGCAACTGCC TATCATTAAA 
GGCTTTCTTG ATGAAAAACG AGTAGAAAGA TTAGAAAAGT ACCTTGGGTC TGAGAATGTC 
CTCTTAATGG AATCCACATT CCATGACATG GAAAGTAAGC TAGAGCTTGC TCAAAAGGAG 
AGTCTGGCAT ATAATATGGA AAAAGCCCTG GAAGATGTCT TTCGCTCTTC AGAATCAAAC 
ATCCTTGATA TGGTAGAGAA AATGTTGGAT TCAAGAGTGG CAGAAAACAA GGAACTGGGC 
ATGAAGGAGA TAGACATGTT TGATGAAGAG GCTGCTCTGC TGGATGATAT TCAGGACCTG 
ATCTATTTTG TCAGATACAA ACACCCTTTG GTAGAAGAGA GTGTACCACT AGCAGCTGCT 
GAGCCTCCAA AACAAAGTTC TGATGTGCCT CTTGCAGTAT TTCCTATAGA ACAAAAAACA 
GAACCCAAAG GAGACCAGAG AAGAAGGTTT TCAGTGAATG AAGGAGATAC ATTGGCACCA 
GAGGAAAACA AGAGGCCACC AGAGAGTATA GAAATAAAAG TTCATAAAGT GGAAGTACCA 
GAGGAGGATA TGGACATCTC AGAAAAGGAT GATCTTTTGG ATTCAGGAGA GATCAGAGAT 
ATCAGTGACT CAGAAGATAG ACTACTGGTT AAAGAGTCCT CCCCAGACCA AAGTATTGTG 
GAAGATTTGG CTAGAGGAAT GGTTTCAGTA GAAGATACTC CTTTGGATAC AAAACAACAT 
CCTCTTGAAA TAATTTCCAC AGAAGAAACT CCTTTAGATA CAGAACAACA TCCTCTAGGT 
ACAGTTTCCA CAGAAGAGAC TCCTTTGGAT ACAGAACAAC AACTAGAAAC AAATCCAGAT 
GTGCCACTGA TTCTTGCAGC TATGAAGAGT GCAAGTCTTG TAGCAAAAGA AAGCATGAAA 
CAATATACTG AGATGTTGAT TGCTACATTA CCTGAGGATA TTCGACCTGG TCCTGATTTT 
CGTGGAATGC CCTGGGAGCC TATAATTATT ACAGTCTTAC TGGGAATTGT TTCACTTGCC 
ATTTTCTTCT GGAGAACGTT TCTTTCAGTG AAGAGTAGAG TTTATCAAGT GACTGAAAAA 
CAGCTTGCTG AGAAGATTAA AACTCTTTTA CAAGAAAAAA CAGAGATTTT AGAAAAAATA 
TCAGAATATG ATCAGAAGAT CAAAAAAGCA AAGGAGTCAG TGAAGGAAAC CAAGAAACAA 
AATATTAATC TTTCTGATGA AGCAGCTGGC CTTAAGGATA AAATGAGAGG GCTTGAAGAA 
ACAAACCAAA AACTGGGTGA CAAAGTGAAA AATCTCCATT CATTGTTGGA AACTGAGAAA 
GAACAGAATG TAAAGAAACA GGACATGATA TTGGAAACTC AGAAATCAAT AGAAAAGCTA 
CAAGAAGTTA TTGCAATGCA TTCTGTTGAA CTTTCAGAGG TGCAGATAGC CCTTAATGAA 
GCTAAGTTCA GTGAAGAGAA AGTAAAGTCT GAACTGCATC ATGTGCAAGA AGAAAATGCA 
CGACTCAAAA AGAGAAAGGA GCAGCTGCTG CAGGAAGCAG AAGGTTGGAG TGAGCGACAC 
ACTGAACTCA GTGAGCAAAT CAAATTATAT CAGAAGACTC AGAAAGACAC CGAAGAAGCC 
CTTGCTTACA AAGAGAATGA AATTGAAGTT TTGACTAATT GCATTATGCA GCTGAAACAA 
CTTGATCTTG ATTCTGAATC TGAGCGCAAA AATAATGAAG AAGGAAATGG ATGGGATGAC 
TTAGCCAATG GAGAACTGTC AGATAAAATC ACCTCAGATA ATCGGAATGA GAAGATGAAG 
AGCCAGATTA AACAGATGAT GGATGTCTCA CGGGTTAAAA CTACATTAAC AATAGTTGAA 
GAAGATAGAA ACCAATTGCA GTCCAAACTC AGTGATGAAA TAAAAGCTCG GCATGAATTG 
GAAGAACAAA TTAAGAAGTT GGAACATGAC TCTTCTTCTC TGCAGTCAGC CAAAGCACAG 
TTGGAAAATG AATGTAAAAC TCTGCAACAA AAAGTGGAGA TTCTTAATGA ACTCTACCAG 
CAGAAAGAGA TGGTTCTGCA GAAGAAGCTC ACTCAGGAAG AGTATGAAAG GCAAGAAAAG 
GAGCAAAAGC TCACTGCTGC AGATGAGAAA GTCAGCTTGG TTGCAGAAGA AGTGAAAACT 
TATAAGCAAA GAATTCAGGA GATGGAGGAT GAATTACAGA AAACAGAGAG GTCATATAAG 
AATCAGATTG CTAGTCATGA GAAGAAGGCC CATGACAACT GGCTCATTGC TCGTGCTGCA 
GAAAGGGCTC TTGCAGAGGA GAAGAGGGAA GCAGCCAACC TGAGACAGAA ATTAATAGAA 
GTGAATCAAA AGATTGCAAT GCTCCAAAGA CCAATGATTG TAAAGCCAAC TCCAGGAAGA 
CCTGATCATC AGATTCCGCC ACGGAGAGGG CCCCTCAGCC GAGACGGCTC TTTTGGTCCA 
TCCCCTGTGA GTGGTGGTGC ACCCTCCCCA CCACTGATGA TAGAACCGCC TATACGGTCA 
GTCTCTGCTA CTCTTAACCG AAGAGATGTA CCTAGAAGTG AATTTGGATC TATGGATGGG 
CCTCCTCTTG GTCCTAGTCT GAGGAGGTCA TCTGAAGTAT CTGGAAGAAC GTCTGCCTCT 
GATTTGGGTC CTGGTCCAGT CCCTTTGATG AATAGTGGCC CAAGAAGCTC TTCTCCCTCA 
ACAGTGCTGG ATGGCATGGT TAACACTGGT CCCAAAGGCC ACCCACCATT CCCAGGAATG 
CCCGTCATGT CACCAATAGG AGGACCTCCA CCACCACCTC TTCGATTTGG ACCACCTCCT 
CCACTACGAG GACCTTATGG GCCTCGACCT CTTCCTCCAC CTCTTGTTCC TGGTGGTCCT 
CCACCTCCAA GCTTTCGGGA GTACTTACAT GGCCTTCCAC CAGGAGTAAG GGACCTACCT 
CCTGACCCTC GAGAGTATAT ACGTGGACAC CCACCTTTTG GACCTCTAGG CCCTCCTGGC 
CCAAGAGAGT ACCTTCCTCC TGGCCCACGA CTGCCGCCTC CAGCCCATGG TCCAAGGGAC 
TACCCACCTC CACCTTTTAC AAGAGACTTG ACACCTTCAG GTTCTAGAGA CTGTGCCCCC 
CCTCATCCAC CTCATGCCTC TCAAGGAAGC ACACAGGACT ATGCACAGGC TCCAGAACAG 
AGCCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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見積依頼

Eメール

[email protected]

電話

03-6811-6572

FAX

03-6811-6573
050-3174-7589(受注専用)

オンラインフォーム

オンライン注文

MIA3 cDNA ORF clone, Phascolarctos cinereus(koala)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

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