NCKAP1 cDNA ORF clone, Pelodiscus sinensis(Chinese soft-shelled turtle)

Gene
Clones
gRNAs

Summary

Gene Symbol	NCKAP1
Entrez Gene ID	102454882
Full Name	NCK associated protein 1
Gene Type	protein-coding
Organism	Pelodiscus sinensis(Chinese soft-shelled turtle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_014572871.1	XP_014428357.1	nck-associated protein 1 isoform X2
XM_014572870.1	XP_014428356.1	nck-associated protein 1 isoform X1

Pathways from BioSystems
Gene Ontology

The following NCKAP1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NCKAP1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPe28086	XM_014572870.1 Latest version!	Pelodiscus sinensis NCK associated protein 1 (NCKAP1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OPe28087	XM_014572871.1 Latest version!	Pelodiscus sinensis NCK associated protein 1 (NCKAP1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NCKAP1 ( XM_014572870.1 ) cDNA ORF clone, Pelodiscus sinensis(Chinese soft-shelled turtle) -> XP_014428356.1 Pelodiscus sinensis NCK associated protein 1 (NCKAP1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OPe28086
Clone ID Related Accession (Same CDS sequence)	XM_014572870.1
Accession Version	XM_014572870.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3336bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-03
Organism	Pelodiscus sinensis(Chinese soft-shelled turtle)
Product	nck-associated protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005854785.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pelodiscus sinensis Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGTAT TTTTAGTTCA CTTTCCTTTT TTTTTTTTTT TTTTTTTTTT TTGGGAGGCA 
TGTGGAGACC CAAAAGCAAA ACCATCATAT CTTATCGACA AAAACCTGGA ATCTGCTGTC 
AAATTCATTG TGAGAAAGTT CCCAGCTGTA GAAACACGCA ACAACAATCA ACAGCTTGCT 
CAGCTGCAGA AGGAAAAATC AGAGATTCTG AAGAATCTGG CTTTGTATTA CTTCACATTT 
GTTGATGTTA TGGAATTTAA GGACCATGTT TGTGAGCTGC TGAATACTAT TGATGTTTGC 
CAAGTCTTCT TCGACATTAC TGTAAACTTT GATTTAACGA AGAACTACCT AGATTTGATA 
ATAACCTACA CAACACTAAT GATATTGCTG TCTCGAATTG AGGAAAGGAA GGCAATCATT 
GGATTGTACA ATTATGCTCA TGAAATGACA CATGGAGCAA GTGATAGAGA ATATCCACGT 
CTTGGTCAGA TGATTGTGGA TTATGAGAAT CCTTTAAAGA AGATGATGGA GGAATTTGTG 
CCTCACAGCA AATCCCTTTC AGATGCTCTA ATTTCACTCC AGATGGTCTA TCCCAGAAGA 
AATTTGTCAG CTGACCAGTG GAGGAATGCG CAACTATTGA GCCTAATCAG TGCCCCGAGT 
ACAATGCTTA ATCCTGCACA GTCTGACACA ATGCCATGTG AATACCTCTC TCTGGATGCA 
ATGGAAAAAT GGATTATTTT TGGGTTTATT TTGTGCCATG GAATCCTGAA CAGTGATGCC 
ACAGCTTTGA ACCTTTGGAA ACTTGCCCTT CAGAGCAGTT CGTGCCTGTC CCTATTTCGA 
GATGAAGTTT TCCATATACA CAAAGCTGCA GAAGACTTGT TTGTGAACAT AAGAGGCTAC 
AACAAACGTA TTAATGACAT AAGAGAGTGC AAAGAAGCTG CTGTATCACA CTCTGGATCA 
ATGCACAGAG AAAGACGCAA GTTTTTACGG TCTGCACTGA AAGAGCTAGC TACTGTTCTG 
TCCGATCAGC CAGGCTTGTT AGGTCCCAAG GCACTTTTTG TATTTATGGC ATTATCCTTT 
GCTCGTGATG AAATTATTTG GCTTCTTCGC CATGCAGATA ACATGCCTAA GAAGAGTGCA 
GATGATTTCA TAGATAAGCA CATAGCTGAA CTGATCTTCT ACATGGAAGA GCTCAGAGCT 
CATGTGCGAA AATATGGACC AGTGATGCAG AGGTATTATG TGCAGTATCT CTCAGGTTTT 
GATGCTGTGG TCTTAAATGA ACTTGTGCAG AATCTTTCAG TGTGCCCTGA AGATGAATCA 
ATCATCATGT CCTCCTTTGT AAACACTATG ACTTCTCTGA GTGTGAAACA AGTTGAAGAT 
GGGGAGGTAT TTGACTTCAG AGGAATGAGA TTAGATTGGT TTAGATTGCA GGCTTACACC 
AGTGTGTCTA AAGCTTCACT TAGCCTTGCA GACCACAGAG AGCTTGGAAA GATGATGAAC 
ACAATTATTT TCCACACAAA AATGGTAGAT TCCTTGGTGG AGATGTTGGT GGAAACATCT 
GATCTTTCTA TATTTTGTTT TTATAGTCGT GCTTTTGAGA AGATGTTTCA GCAGTGCTTG 
GAGCTGCCCT CTCAGTCGAG ATATTCTATT GCATTTCCAC TGCTTTGCAC TCATTTTATG 
AGCTGCACCC ATGAGCTGTG TCCAGAGGAG CGACATCATA TTGGAGATCG CAGTCTTTCC 
TTGTGTAACA TGTTTTTGGA TGAAATGGCT AAGCAAGCTC GAAATCTTAT CACAGATATT 
TGCACAGAAC AGTGTACGCT GAGTGATCAG TTGCTGCCAA AGCATTGTGC CAAAACAATC 
AGTCAAGCAG TGAACAAAAA GTCAAAAAAA CAGACCGGAA AGAAAGGGGA ACCAGAGAGG 
GAGAAACCAG GAGTAGAAAG CATGAGGAAA AACAGACTGG TGGTGACGAA CTTAGACAAA 
CTGCACACTG CACTTTCCGA GCTCTGCTTC TCAATCAATT ACGTACCAAA CATGATTGTC 
TGGGAACATA CATTTACCCC AAGGGAGTAC TTAACATCTC ACCTGGAAAT CCGCTTTACC 
AAGTCAATCG TTGGAATGAC TATGTATAAT CAAGCGACAC AAGAGATTGC AAAACCTTCA 
GAGCTGTTAA CAAGTGTCAG AGCCTACATG ACAGTACTCC AATCAATAGA AAATTATGTG 
CAGATTGACA TCACAAGAGT ATTTAACAAT GTTCTTCTTC AGCAAACCCA GCATTTGGAT 
AGTCATGGGG AGCCAACTAT TACCAGTTTA TACACAAATT GGTATTTAGA AACCTTGCTA 
AGGCAAGTCA GCAATGGGCA TATAGCATAT TTCCCAGCAA TGAAGGCATT TGTGAACTTA 
CCCACAGAAA ATGAGTTGAC ATTTAATGCT GAAGAATACT CTGACATATC AGAAATGAGG 
GCCCTCTCAG AACTACTAGG ACCATATGGC ATGAAGTTCC TAAGTGAAAG TCTTATGTGG 
CACATTTCCT CACAGGTTGC TGAACTTAAG AAACTTGTGG TGGAAAATGT TGAAGTGCTA 
ACACAAATGA GGACCAGTTT TGACAAACCA GACCAGATGG CAGCACTCTT TAAAAGATTA 
TCATCTGTTG ATAGTGTTCT GAAGAGGATG ACCATAATTG GCGTAATCTT ATCTTTTCGC 
TCATTGGCAC AGGAAGCACT TAGAGATGTT TTATCCTACC ACATTCCTTT CCTCGTAAGT 
TCAATTGAAG ACTTTAAGGA TCACATTCCG AGGGAGACTG ACATGAAGGT GGCAATGAAT 
GTATATGAGC TGTCGTCTGC TGCTGGATTG CCATGTGAGA TCGATCCCGC CTTGGTTGTA 
GCACTGTCTT CCCAAAAATC AGAAAATATT AGTCCAGAAG AAGAATATAA AATCGCCTGC 
CTCCTCATGG TCTTTGTGGC AGTCTCCTTG CCAACTTTGG CCAGTAATGT GATGTCTCAA 
TATAGCCCTG CCATTGAAGG GCACTGCAAC AACATTCATT GCTTGGCCAA AGCTATCAAC 
CAGATTGCTG CAGCTTTATT TACCATCCAC AAGGGAAGCA TCGAAGACCG CCTTAAAGAA 
TTTTTGGCTC TTGCATCATC TAGCCTACTG AAAATTGGTC AGGAGACAGA CAAAACTACT 
ACAAGGAACA GAGAATCTGT TTATTTACTG TTAGACATGA TTGTGCAGGA GTCTCCATTC 
CTTACAATGG ATCTCTTGGA GTCTTGTTTC CCTTATGTCT TGTTGCGAAA TGCATACCAT 
GCTGTCTACA AACAAAGTGT CACATCTTCT GCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014428356.1
CDS	16..3351
Translation	MNVFLVHFPFFFFFFFFWEACGDPKAKPSYLIDKNLESAVKFIVRKFPAVETRNNNQQLAQLQKEKSEILKNLALYYFTFVDVMEFKDHVCELLNTIDVCQVFFDITVNFDLTKNYLDLIITYTTLMILLSRIEERKAIIGLYNYAHEMTHGASDREYPRLGQMIVDYENPLKKMMEEFVPHSKSLSDALISLQMVYPRRNLSADQWRNAQLLSLISAPSTMLNPAQSDTMPCEYLSLDAMEKWIIFGFILCHGILNSDATALNLWKLALQSSSCLSLFRDEVFHIHKAAEDLFVNIRGYNKRINDIRECKEAAVSHSGSMHRERRKFLRSALKELATVLSDQPGLLGPKALFVFMALSFARDEIIWLLRHADNMPKKSADDFIDKHIAELIFYMEELRAHVRKYGPVMQRYYVQYLSGFDAVVLNELVQNLSVCPEDESIIMSSFVNTMTSLSVKQVEDGEVFDFRGMRLDWFRLQAYTSVSKASLSLADHRELGKMMNTIIFHTKMVDSLVEMLVETSDLSIFCFYSRAFEKMFQQCLELPSQSRYSIAFPLLCTHFMSCTHELCPEERHHIGDRSLSLCNMFLDEMAKQARNLITDICTEQCTLSDQLLPKHCAKTISQAVNKKSKKQTGKKGEPEREKPGVESMRKNRLVVTNLDKLHTALSELCFSINYVPNMIVWEHTFTPREYLTSHLEIRFTKSIVGMTMYNQATQEIAKPSELLTSVRAYMTVLQSIENYVQIDITRVFNNVLLQQTQHLDSHGEPTITSLYTNWYLETLLRQVSNGHIAYFPAMKAFVNLPTENELTFNAEEYSDISEMRALSELLGPYGMKFLSESLMWHISSQVAELKKLVVENVEVLTQMRTSFDKPDQMAALFKRLSSVDSVLKRMTIIGVILSFRSLAQEALRDVLSYHIPFLVSSIEDFKDHIPRETDMKVAMNVYELSSAAGLPCEIDPALVVALSSQKSENISPEEEYKIACLLMVFVAVSLPTLASNVMSQYSPAIEGHCNNIHCLAKAINQIAAALFTIHKGSIEDRLKEFLALASSSLLKIGQETDKTTTRNRESVYLLLDMIVQESPFLTMDLLESCFPYVLLRNAYHAVYKQSVTSSA

Target ORF information:

RefSeq Version	XM_014572870.1
Organism	Pelodiscus sinensis(Chinese soft-shelled turtle)
Definition	Pelodiscus sinensis NCK associated protein 1 (NCKAP1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014572870.1

ORF Insert Sequence:

ATGAATGTAT TTTTAGTTCA CTTTCCTTTT TTTTTTTTTT TTTTTTTTTT TTGGGAGGCA 
TGTGGAGACC CAAAAGCAAA ACCATCATAT CTTATCGACA AAAACCTGGA ATCTGCTGTC 
AAATTCATTG TGAGAAAGTT CCCAGCTGTA GAAACACGCA ACAACAATCA ACAGCTTGCT 
CAGCTGCAGA AGGAAAAATC AGAGATTCTG AAGAATCTGG CTTTGTATTA CTTCACATTT 
GTTGATGTTA TGGAATTTAA GGACCATGTT TGTGAGCTGC TGAATACTAT TGATGTTTGC 
CAAGTCTTCT TCGACATTAC TGTAAACTTT GATTTAACGA AGAACTACCT AGATTTGATA 
ATAACCTACA CAACACTAAT GATATTGCTG TCTCGAATTG AGGAAAGGAA GGCAATCATT 
GGATTGTACA ATTATGCTCA TGAAATGACA CATGGAGCAA GTGATAGAGA ATATCCACGT 
CTTGGTCAGA TGATTGTGGA TTATGAGAAT CCTTTAAAGA AGATGATGGA GGAATTTGTG 
CCTCACAGCA AATCCCTTTC AGATGCTCTA ATTTCACTCC AGATGGTCTA TCCCAGAAGA 
AATTTGTCAG CTGACCAGTG GAGGAATGCG CAACTATTGA GCCTAATCAG TGCCCCGAGT 
ACAATGCTTA ATCCTGCACA GTCTGACACA ATGCCATGTG AATACCTCTC TCTGGATGCA 
ATGGAAAAAT GGATTATTTT TGGGTTTATT TTGTGCCATG GAATCCTGAA CAGTGATGCC 
ACAGCTTTGA ACCTTTGGAA ACTTGCCCTT CAGAGCAGTT CGTGCCTGTC CCTATTTCGA 
GATGAAGTTT TCCATATACA CAAAGCTGCA GAAGACTTGT TTGTGAACAT AAGAGGCTAC 
AACAAACGTA TTAATGACAT AAGAGAGTGC AAAGAAGCTG CTGTATCACA CTCTGGATCA 
ATGCACAGAG AAAGACGCAA GTTTTTACGG TCTGCACTGA AAGAGCTAGC TACTGTTCTG 
TCCGATCAGC CAGGCTTGTT AGGTCCCAAG GCACTTTTTG TATTTATGGC ATTATCCTTT 
GCTCGTGATG AAATTATTTG GCTTCTTCGC CATGCAGATA ACATGCCTAA GAAGAGTGCA 
GATGATTTCA TAGATAAGCA CATAGCTGAA CTGATCTTCT ACATGGAAGA GCTCAGAGCT 
CATGTGCGAA AATATGGACC AGTGATGCAG AGGTATTATG TGCAGTATCT CTCAGGTTTT 
GATGCTGTGG TCTTAAATGA ACTTGTGCAG AATCTTTCAG TGTGCCCTGA AGATGAATCA 
ATCATCATGT CCTCCTTTGT AAACACTATG ACTTCTCTGA GTGTGAAACA AGTTGAAGAT 
GGGGAGGTAT TTGACTTCAG AGGAATGAGA TTAGATTGGT TTAGATTGCA GGCTTACACC 
AGTGTGTCTA AAGCTTCACT TAGCCTTGCA GACCACAGAG AGCTTGGAAA GATGATGAAC 
ACAATTATTT TCCACACAAA AATGGTAGAT TCCTTGGTGG AGATGTTGGT GGAAACATCT 
GATCTTTCTA TATTTTGTTT TTATAGTCGT GCTTTTGAGA AGATGTTTCA GCAGTGCTTG 
GAGCTGCCCT CTCAGTCGAG ATATTCTATT GCATTTCCAC TGCTTTGCAC TCATTTTATG 
AGCTGCACCC ATGAGCTGTG TCCAGAGGAG CGACATCATA TTGGAGATCG CAGTCTTTCC 
TTGTGTAACA TGTTTTTGGA TGAAATGGCT AAGCAAGCTC GAAATCTTAT CACAGATATT 
TGCACAGAAC AGTGTACGCT GAGTGATCAG TTGCTGCCAA AGCATTGTGC CAAAACAATC 
AGTCAAGCAG TGAACAAAAA GTCAAAAAAA CAGACCGGAA AGAAAGGGGA ACCAGAGAGG 
GAGAAACCAG GAGTAGAAAG CATGAGGAAA AACAGACTGG TGGTGACGAA CTTAGACAAA 
CTGCACACTG CACTTTCCGA GCTCTGCTTC TCAATCAATT ACGTACCAAA CATGATTGTC 
TGGGAACATA CATTTACCCC AAGGGAGTAC TTAACATCTC ACCTGGAAAT CCGCTTTACC 
AAGTCAATCG TTGGAATGAC TATGTATAAT CAAGCGACAC AAGAGATTGC AAAACCTTCA 
GAGCTGTTAA CAAGTGTCAG AGCCTACATG ACAGTACTCC AATCAATAGA AAATTATGTG 
CAGATTGACA TCACAAGAGT ATTTAACAAT GTTCTTCTTC AGCAAACCCA GCATTTGGAT 
AGTCATGGGG AGCCAACTAT TACCAGTTTA TACACAAATT GGTATTTAGA AACCTTGCTA 
AGGCAAGTCA GCAATGGGCA TATAGCATAT TTCCCAGCAA TGAAGGCATT TGTGAACTTA 
CCCACAGAAA ATGAGTTGAC ATTTAATGCT GAAGAATACT CTGACATATC AGAAATGAGG 
GCCCTCTCAG AACTACTAGG ACCATATGGC ATGAAGTTCC TAAGTGAAAG TCTTATGTGG 
CACATTTCCT CACAGGTTGC TGAACTTAAG AAACTTGTGG TGGAAAATGT TGAAGTGCTA 
ACACAAATGA GGACCAGTTT TGACAAACCA GACCAGATGG CAGCACTCTT TAAAAGATTA 
TCATCTGTTG ATAGTGTTCT GAAGAGGATG ACCATAATTG GCGTAATCTT ATCTTTTCGC 
TCATTGGCAC AGGAAGCACT TAGAGATGTT TTATCCTACC ACATTCCTTT CCTCGTAAGT 
TCAATTGAAG ACTTTAAGGA TCACATTCCG AGGGAGACTG ACATGAAGGT GGCAATGAAT 
GTATATGAGC TGTCGTCTGC TGCTGGATTG CCATGTGAGA TCGATCCCGC CTTGGTTGTA 
GCACTGTCTT CCCAAAAATC AGAAAATATT AGTCCAGAAG AAGAATATAA AATCGCCTGC 
CTCCTCATGG TCTTTGTGGC AGTCTCCTTG CCAACTTTGG CCAGTAATGT GATGTCTCAA 
TATAGCCCTG CCATTGAAGG GCACTGCAAC AACATTCATT GCTTGGCCAA AGCTATCAAC 
CAGATTGCTG CAGCTTTATT TACCATCCAC AAGGGAAGCA TCGAAGACCG CCTTAAAGAA 
TTTTTGGCTC TTGCATCATC TAGCCTACTG AAAATTGGTC AGGAGACAGA CAAAACTACT 
ACAAGGAACA GAGAATCTGT TTATTTACTG TTAGACATGA TTGTGCAGGA GTCTCCATTC 
CTTACAATGG ATCTCTTGGA GTCTTGTTTC CCTTATGTCT TGTTGCGAAA TGCATACCAT 
GCTGTCTACA AACAAAGTGT CACATCTTCT GCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NCKAP1 ( XM_014572871.1 ) cDNA ORF clone, Pelodiscus sinensis(Chinese soft-shelled turtle) -> XP_014428357.1 Pelodiscus sinensis NCK associated protein 1 (NCKAP1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OPe28087
Clone ID Related Accession (Same CDS sequence)	XM_014572871.1
Accession Version	XM_014572871.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3330bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-03
Organism	Pelodiscus sinensis(Chinese soft-shelled turtle)
Product	nck-associated protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005854785.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pelodiscus sinensis Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGTAT TTTTAGTTCA CTTTCCTTTT TTTTTTTTTT TTTTTTTTTT TTGGGAGGCA 
TGTGGAGACC CAAAAGCAAA ACCATCATAT CTTATCGACA AAAACCTGGA ATCTGCTGTC 
AAATTCATTG TGAGAAAGTT CCCAGCTGTA GAAACACGCA ACAACAATCT TGCTCAGCTG 
CAGAAGGAAA AATCAGAGAT TCTGAAGAAT CTGGCTTTGT ATTACTTCAC ATTTGTTGAT 
GTTATGGAAT TTAAGGACCA TGTTTGTGAG CTGCTGAATA CTATTGATGT TTGCCAAGTC 
TTCTTCGACA TTACTGTAAA CTTTGATTTA ACGAAGAACT ACCTAGATTT GATAATAACC 
TACACAACAC TAATGATATT GCTGTCTCGA ATTGAGGAAA GGAAGGCAAT CATTGGATTG 
TACAATTATG CTCATGAAAT GACACATGGA GCAAGTGATA GAGAATATCC ACGTCTTGGT 
CAGATGATTG TGGATTATGA GAATCCTTTA AAGAAGATGA TGGAGGAATT TGTGCCTCAC 
AGCAAATCCC TTTCAGATGC TCTAATTTCA CTCCAGATGG TCTATCCCAG AAGAAATTTG 
TCAGCTGACC AGTGGAGGAA TGCGCAACTA TTGAGCCTAA TCAGTGCCCC GAGTACAATG 
CTTAATCCTG CACAGTCTGA CACAATGCCA TGTGAATACC TCTCTCTGGA TGCAATGGAA 
AAATGGATTA TTTTTGGGTT TATTTTGTGC CATGGAATCC TGAACAGTGA TGCCACAGCT 
TTGAACCTTT GGAAACTTGC CCTTCAGAGC AGTTCGTGCC TGTCCCTATT TCGAGATGAA 
GTTTTCCATA TACACAAAGC TGCAGAAGAC TTGTTTGTGA ACATAAGAGG CTACAACAAA 
CGTATTAATG ACATAAGAGA GTGCAAAGAA GCTGCTGTAT CACACTCTGG ATCAATGCAC 
AGAGAAAGAC GCAAGTTTTT ACGGTCTGCA CTGAAAGAGC TAGCTACTGT TCTGTCCGAT 
CAGCCAGGCT TGTTAGGTCC CAAGGCACTT TTTGTATTTA TGGCATTATC CTTTGCTCGT 
GATGAAATTA TTTGGCTTCT TCGCCATGCA GATAACATGC CTAAGAAGAG TGCAGATGAT 
TTCATAGATA AGCACATAGC TGAACTGATC TTCTACATGG AAGAGCTCAG AGCTCATGTG 
CGAAAATATG GACCAGTGAT GCAGAGGTAT TATGTGCAGT ATCTCTCAGG TTTTGATGCT 
GTGGTCTTAA ATGAACTTGT GCAGAATCTT TCAGTGTGCC CTGAAGATGA ATCAATCATC 
ATGTCCTCCT TTGTAAACAC TATGACTTCT CTGAGTGTGA AACAAGTTGA AGATGGGGAG 
GTATTTGACT TCAGAGGAAT GAGATTAGAT TGGTTTAGAT TGCAGGCTTA CACCAGTGTG 
TCTAAAGCTT CACTTAGCCT TGCAGACCAC AGAGAGCTTG GAAAGATGAT GAACACAATT 
ATTTTCCACA CAAAAATGGT AGATTCCTTG GTGGAGATGT TGGTGGAAAC ATCTGATCTT 
TCTATATTTT GTTTTTATAG TCGTGCTTTT GAGAAGATGT TTCAGCAGTG CTTGGAGCTG 
CCCTCTCAGT CGAGATATTC TATTGCATTT CCACTGCTTT GCACTCATTT TATGAGCTGC 
ACCCATGAGC TGTGTCCAGA GGAGCGACAT CATATTGGAG ATCGCAGTCT TTCCTTGTGT 
AACATGTTTT TGGATGAAAT GGCTAAGCAA GCTCGAAATC TTATCACAGA TATTTGCACA 
GAACAGTGTA CGCTGAGTGA TCAGTTGCTG CCAAAGCATT GTGCCAAAAC AATCAGTCAA 
GCAGTGAACA AAAAGTCAAA AAAACAGACC GGAAAGAAAG GGGAACCAGA GAGGGAGAAA 
CCAGGAGTAG AAAGCATGAG GAAAAACAGA CTGGTGGTGA CGAACTTAGA CAAACTGCAC 
ACTGCACTTT CCGAGCTCTG CTTCTCAATC AATTACGTAC CAAACATGAT TGTCTGGGAA 
CATACATTTA CCCCAAGGGA GTACTTAACA TCTCACCTGG AAATCCGCTT TACCAAGTCA 
ATCGTTGGAA TGACTATGTA TAATCAAGCG ACACAAGAGA TTGCAAAACC TTCAGAGCTG 
TTAACAAGTG TCAGAGCCTA CATGACAGTA CTCCAATCAA TAGAAAATTA TGTGCAGATT 
GACATCACAA GAGTATTTAA CAATGTTCTT CTTCAGCAAA CCCAGCATTT GGATAGTCAT 
GGGGAGCCAA CTATTACCAG TTTATACACA AATTGGTATT TAGAAACCTT GCTAAGGCAA 
GTCAGCAATG GGCATATAGC ATATTTCCCA GCAATGAAGG CATTTGTGAA CTTACCCACA 
GAAAATGAGT TGACATTTAA TGCTGAAGAA TACTCTGACA TATCAGAAAT GAGGGCCCTC 
TCAGAACTAC TAGGACCATA TGGCATGAAG TTCCTAAGTG AAAGTCTTAT GTGGCACATT 
TCCTCACAGG TTGCTGAACT TAAGAAACTT GTGGTGGAAA ATGTTGAAGT GCTAACACAA 
ATGAGGACCA GTTTTGACAA ACCAGACCAG ATGGCAGCAC TCTTTAAAAG ATTATCATCT 
GTTGATAGTG TTCTGAAGAG GATGACCATA ATTGGCGTAA TCTTATCTTT TCGCTCATTG 
GCACAGGAAG CACTTAGAGA TGTTTTATCC TACCACATTC CTTTCCTCGT AAGTTCAATT 
GAAGACTTTA AGGATCACAT TCCGAGGGAG ACTGACATGA AGGTGGCAAT GAATGTATAT 
GAGCTGTCGT CTGCTGCTGG ATTGCCATGT GAGATCGATC CCGCCTTGGT TGTAGCACTG 
TCTTCCCAAA AATCAGAAAA TATTAGTCCA GAAGAAGAAT ATAAAATCGC CTGCCTCCTC 
ATGGTCTTTG TGGCAGTCTC CTTGCCAACT TTGGCCAGTA ATGTGATGTC TCAATATAGC 
CCTGCCATTG AAGGGCACTG CAACAACATT CATTGCTTGG CCAAAGCTAT CAACCAGATT 
GCTGCAGCTT TATTTACCAT CCACAAGGGA AGCATCGAAG ACCGCCTTAA AGAATTTTTG 
GCTCTTGCAT CATCTAGCCT ACTGAAAATT GGTCAGGAGA CAGACAAAAC TACTACAAGG 
AACAGAGAAT CTGTTTATTT ACTGTTAGAC ATGATTGTGC AGGAGTCTCC ATTCCTTACA 
ATGGATCTCT TGGAGTCTTG TTTCCCTTAT GTCTTGTTGC GAAATGCATA CCATGCTGTC 
TACAAACAAA GTGTCACATC TTCTGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014428357.1
CDS	16..3345
Translation	MNVFLVHFPFFFFFFFFWEACGDPKAKPSYLIDKNLESAVKFIVRKFPAVETRNNNLAQLQKEKSEILKNLALYYFTFVDVMEFKDHVCELLNTIDVCQVFFDITVNFDLTKNYLDLIITYTTLMILLSRIEERKAIIGLYNYAHEMTHGASDREYPRLGQMIVDYENPLKKMMEEFVPHSKSLSDALISLQMVYPRRNLSADQWRNAQLLSLISAPSTMLNPAQSDTMPCEYLSLDAMEKWIIFGFILCHGILNSDATALNLWKLALQSSSCLSLFRDEVFHIHKAAEDLFVNIRGYNKRINDIRECKEAAVSHSGSMHRERRKFLRSALKELATVLSDQPGLLGPKALFVFMALSFARDEIIWLLRHADNMPKKSADDFIDKHIAELIFYMEELRAHVRKYGPVMQRYYVQYLSGFDAVVLNELVQNLSVCPEDESIIMSSFVNTMTSLSVKQVEDGEVFDFRGMRLDWFRLQAYTSVSKASLSLADHRELGKMMNTIIFHTKMVDSLVEMLVETSDLSIFCFYSRAFEKMFQQCLELPSQSRYSIAFPLLCTHFMSCTHELCPEERHHIGDRSLSLCNMFLDEMAKQARNLITDICTEQCTLSDQLLPKHCAKTISQAVNKKSKKQTGKKGEPEREKPGVESMRKNRLVVTNLDKLHTALSELCFSINYVPNMIVWEHTFTPREYLTSHLEIRFTKSIVGMTMYNQATQEIAKPSELLTSVRAYMTVLQSIENYVQIDITRVFNNVLLQQTQHLDSHGEPTITSLYTNWYLETLLRQVSNGHIAYFPAMKAFVNLPTENELTFNAEEYSDISEMRALSELLGPYGMKFLSESLMWHISSQVAELKKLVVENVEVLTQMRTSFDKPDQMAALFKRLSSVDSVLKRMTIIGVILSFRSLAQEALRDVLSYHIPFLVSSIEDFKDHIPRETDMKVAMNVYELSSAAGLPCEIDPALVVALSSQKSENISPEEEYKIACLLMVFVAVSLPTLASNVMSQYSPAIEGHCNNIHCLAKAINQIAAALFTIHKGSIEDRLKEFLALASSSLLKIGQETDKTTTRNRESVYLLLDMIVQESPFLTMDLLESCFPYVLLRNAYHAVYKQSVTSSA

Target ORF information:

RefSeq Version	XM_014572871.1
Organism	Pelodiscus sinensis(Chinese soft-shelled turtle)
Definition	Pelodiscus sinensis NCK associated protein 1 (NCKAP1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014572871.1

ORF Insert Sequence:

ATGAATGTAT TTTTAGTTCA CTTTCCTTTT TTTTTTTTTT TTTTTTTTTT TTGGGAGGCA 
TGTGGAGACC CAAAAGCAAA ACCATCATAT CTTATCGACA AAAACCTGGA ATCTGCTGTC 
AAATTCATTG TGAGAAAGTT CCCAGCTGTA GAAACACGCA ACAACAATCT TGCTCAGCTG 
CAGAAGGAAA AATCAGAGAT TCTGAAGAAT CTGGCTTTGT ATTACTTCAC ATTTGTTGAT 
GTTATGGAAT TTAAGGACCA TGTTTGTGAG CTGCTGAATA CTATTGATGT TTGCCAAGTC 
TTCTTCGACA TTACTGTAAA CTTTGATTTA ACGAAGAACT ACCTAGATTT GATAATAACC 
TACACAACAC TAATGATATT GCTGTCTCGA ATTGAGGAAA GGAAGGCAAT CATTGGATTG 
TACAATTATG CTCATGAAAT GACACATGGA GCAAGTGATA GAGAATATCC ACGTCTTGGT 
CAGATGATTG TGGATTATGA GAATCCTTTA AAGAAGATGA TGGAGGAATT TGTGCCTCAC 
AGCAAATCCC TTTCAGATGC TCTAATTTCA CTCCAGATGG TCTATCCCAG AAGAAATTTG 
TCAGCTGACC AGTGGAGGAA TGCGCAACTA TTGAGCCTAA TCAGTGCCCC GAGTACAATG 
CTTAATCCTG CACAGTCTGA CACAATGCCA TGTGAATACC TCTCTCTGGA TGCAATGGAA 
AAATGGATTA TTTTTGGGTT TATTTTGTGC CATGGAATCC TGAACAGTGA TGCCACAGCT 
TTGAACCTTT GGAAACTTGC CCTTCAGAGC AGTTCGTGCC TGTCCCTATT TCGAGATGAA 
GTTTTCCATA TACACAAAGC TGCAGAAGAC TTGTTTGTGA ACATAAGAGG CTACAACAAA 
CGTATTAATG ACATAAGAGA GTGCAAAGAA GCTGCTGTAT CACACTCTGG ATCAATGCAC 
AGAGAAAGAC GCAAGTTTTT ACGGTCTGCA CTGAAAGAGC TAGCTACTGT TCTGTCCGAT 
CAGCCAGGCT TGTTAGGTCC CAAGGCACTT TTTGTATTTA TGGCATTATC CTTTGCTCGT 
GATGAAATTA TTTGGCTTCT TCGCCATGCA GATAACATGC CTAAGAAGAG TGCAGATGAT 
TTCATAGATA AGCACATAGC TGAACTGATC TTCTACATGG AAGAGCTCAG AGCTCATGTG 
CGAAAATATG GACCAGTGAT GCAGAGGTAT TATGTGCAGT ATCTCTCAGG TTTTGATGCT 
GTGGTCTTAA ATGAACTTGT GCAGAATCTT TCAGTGTGCC CTGAAGATGA ATCAATCATC 
ATGTCCTCCT TTGTAAACAC TATGACTTCT CTGAGTGTGA AACAAGTTGA AGATGGGGAG 
GTATTTGACT TCAGAGGAAT GAGATTAGAT TGGTTTAGAT TGCAGGCTTA CACCAGTGTG 
TCTAAAGCTT CACTTAGCCT TGCAGACCAC AGAGAGCTTG GAAAGATGAT GAACACAATT 
ATTTTCCACA CAAAAATGGT AGATTCCTTG GTGGAGATGT TGGTGGAAAC ATCTGATCTT 
TCTATATTTT GTTTTTATAG TCGTGCTTTT GAGAAGATGT TTCAGCAGTG CTTGGAGCTG 
CCCTCTCAGT CGAGATATTC TATTGCATTT CCACTGCTTT GCACTCATTT TATGAGCTGC 
ACCCATGAGC TGTGTCCAGA GGAGCGACAT CATATTGGAG ATCGCAGTCT TTCCTTGTGT 
AACATGTTTT TGGATGAAAT GGCTAAGCAA GCTCGAAATC TTATCACAGA TATTTGCACA 
GAACAGTGTA CGCTGAGTGA TCAGTTGCTG CCAAAGCATT GTGCCAAAAC AATCAGTCAA 
GCAGTGAACA AAAAGTCAAA AAAACAGACC GGAAAGAAAG GGGAACCAGA GAGGGAGAAA 
CCAGGAGTAG AAAGCATGAG GAAAAACAGA CTGGTGGTGA CGAACTTAGA CAAACTGCAC 
ACTGCACTTT CCGAGCTCTG CTTCTCAATC AATTACGTAC CAAACATGAT TGTCTGGGAA 
CATACATTTA CCCCAAGGGA GTACTTAACA TCTCACCTGG AAATCCGCTT TACCAAGTCA 
ATCGTTGGAA TGACTATGTA TAATCAAGCG ACACAAGAGA TTGCAAAACC TTCAGAGCTG 
TTAACAAGTG TCAGAGCCTA CATGACAGTA CTCCAATCAA TAGAAAATTA TGTGCAGATT 
GACATCACAA GAGTATTTAA CAATGTTCTT CTTCAGCAAA CCCAGCATTT GGATAGTCAT 
GGGGAGCCAA CTATTACCAG TTTATACACA AATTGGTATT TAGAAACCTT GCTAAGGCAA 
GTCAGCAATG GGCATATAGC ATATTTCCCA GCAATGAAGG CATTTGTGAA CTTACCCACA 
GAAAATGAGT TGACATTTAA TGCTGAAGAA TACTCTGACA TATCAGAAAT GAGGGCCCTC 
TCAGAACTAC TAGGACCATA TGGCATGAAG TTCCTAAGTG AAAGTCTTAT GTGGCACATT 
TCCTCACAGG TTGCTGAACT TAAGAAACTT GTGGTGGAAA ATGTTGAAGT GCTAACACAA 
ATGAGGACCA GTTTTGACAA ACCAGACCAG ATGGCAGCAC TCTTTAAAAG ATTATCATCT 
GTTGATAGTG TTCTGAAGAG GATGACCATA ATTGGCGTAA TCTTATCTTT TCGCTCATTG 
GCACAGGAAG CACTTAGAGA TGTTTTATCC TACCACATTC CTTTCCTCGT AAGTTCAATT 
GAAGACTTTA AGGATCACAT TCCGAGGGAG ACTGACATGA AGGTGGCAAT GAATGTATAT 
GAGCTGTCGT CTGCTGCTGG ATTGCCATGT GAGATCGATC CCGCCTTGGT TGTAGCACTG 
TCTTCCCAAA AATCAGAAAA TATTAGTCCA GAAGAAGAAT ATAAAATCGC CTGCCTCCTC 
ATGGTCTTTG TGGCAGTCTC CTTGCCAACT TTGGCCAGTA ATGTGATGTC TCAATATAGC 
CCTGCCATTG AAGGGCACTG CAACAACATT CATTGCTTGG CCAAAGCTAT CAACCAGATT 
GCTGCAGCTT TATTTACCAT CCACAAGGGA AGCATCGAAG ACCGCCTTAA AGAATTTTTG 
GCTCTTGCAT CATCTAGCCT ACTGAAAATT GGTCAGGAGA CAGACAAAAC TACTACAAGG 
AACAGAGAAT CTGTTTATTT ACTGTTAGAC ATGATTGTGC AGGAGTCTCC ATTCCTTACA 
ATGGATCTCT TGGAGTCTTG TTTCCCTTAT GTCTTGTTGC GAAATGCATA CCATGCTGTC 
TACAAACAAA GTGTCACATC TTCTGCATAA