PPFIA1 gene cDNA ORF clone, Parus major(Great Tit)

mRNA	Protein	Name
XM_015631372.2	XP_015486858.1	liprin-alpha-1 isoform X5
XM_015631380.2	XP_015486866.1	liprin-alpha-1 isoform X12
XM_015631374.2	XP_015486860.1	liprin-alpha-1 isoform X7
XM_015631370.2	XP_015486856.1	liprin-alpha-1 isoform X3
XM_015631373.2	XP_015486859.1	liprin-alpha-1 isoform X6
XM_015631368.2	XP_015486854.1	liprin-alpha-1 isoform X1
XM_015631369.2	XP_015486855.1	liprin-alpha-1 isoform X2
XM_015631371.2	XP_015486857.1	liprin-alpha-1 isoform X4
XM_015631375.2	XP_015486861.1	liprin-alpha-1 isoform X8
XM_015631377.2	XP_015486863.1	liprin-alpha-1 isoform X9
XM_015631378.2	XP_015486864.1	liprin-alpha-1 isoform X10
XM_015631381.2	XP_015486867.1	liprin-alpha-1 isoform X13
XM_015631379.2	XP_015486865.1	liprin-alpha-1 isoform X11

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OPa179668	XM_015631368.2 Latest version!	Parus major PTPRF interacting protein alpha 1 (PPFIA1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OPa179669	XM_015631369.2 Latest version!	Parus major PTPRF interacting protein alpha 1 (PPFIA1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OPa179670	XM_015631370.2 Latest version!	Parus major PTPRF interacting protein alpha 1 (PPFIA1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OPa179671	XM_015631371.2 Latest version!	Parus major PTPRF interacting protein alpha 1 (PPFIA1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OPa179672	XM_015631372.2 Latest version!	Parus major PTPRF interacting protein alpha 1 (PPFIA1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OPa179673	XM_015631373.2 Latest version!	Parus major PTPRF interacting protein alpha 1 (PPFIA1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OPa179674	XM_015631374.2 Latest version!	Parus major PTPRF interacting protein alpha 1 (PPFIA1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OPa179675	XM_015631375.2 Latest version!	Parus major PTPRF interacting protein alpha 1 (PPFIA1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OPa179676	XM_015631377.2 Latest version!	Parus major PTPRF interacting protein alpha 1 (PPFIA1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OPa179677	XM_015631378.2 Latest version!	Parus major PTPRF interacting protein alpha 1 (PPFIA1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OPa179678	XM_015631379.2 Latest version!	Parus major PTPRF interacting protein alpha 1 (PPFIA1), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OPa179679	XM_015631380.2 Latest version!	Parus major PTPRF interacting protein alpha 1 (PPFIA1), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OPa179680	XM_015631381.2 Latest version!	Parus major PTPRF interacting protein alpha 1 (PPFIA1), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00

PPFIA1 ( XM_015631368.2 ) cDNA ORF clone, Parus major(Great Tit) -> XP_015486854.1 Parus major PTPRF interacting protein alpha 1 (PPFIA1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OPa179668
Clone ID Related Accession (Same CDS sequence)	XM_015631368.2
Accession Version	XM_015631368.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3801bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-11-13
Organism	Parus major(Great Tit)
Product	liprin-alpha-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_031774.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Nov 4, 2016 this sequence version replaced XM_015631368.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Parus major Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTGTG AGGTGATGCC AACCATCAGT GAGGCCGAGA TTCCCGCCGG GGGAAATGGA 
GGCCACGGTT CAGGTTCCCC GTTGCAATCA GATGCTGATT CCCACTTCGA GCAGTTAATG 
GTGTCCATGC TGGAAGAAAG GGATCGCCTC CTAGAAACGC TGCGAGAAAC TCAAGAGACG 
CTTGCCCTCA CCCAGGGCAA GCTGCATGAG GTTGGCCATG AGAGGGATTC CTTGCAGAGG 
CAGCTCAATA CTGCACTTCC ACAGGAATTT GCAGCACTCA CCAAAGAGCT GAATGTATGC 
AGGGAGCAGC TGCTTGAAAG GGAAGAAGAA ATTGCTGAGC TGAAAGCAGA AAGAAATAAT 
ACGAGGCTAT TACTGGAACA TTTGGAGTGT CTTGTCTCCA GGCATGAGCG ATCCCTCAGG 
ATGACTGTTG TGAAGAGGCA AGCTCAGTCT CCAGCAGGAG TTTCTAGTGA AGTAGAAGTT 
CTCAAAGCAC TAAAATCTTT ATTTGAGCAC CACAAAGCCC TTGATGAAAA GGTAAGGGAG 
AGATTACGAG TAGCACTTGA AAGATGTAGC TTATTGGAAG AAGAACTAGG TACTACACAT 
AAAGAGTTAA TGATTCTGAA AGAGCAAAAT AATCAGAAGA AAACACTTCC AGATGGGATG 
CTGGATATAA ATCATGAACA AGAAAATACA CCAACTACAA ATGGGAAGAG ATCCTCTGAT 
GGTTCATTAT GCCATGATGA AAACCTTGCT AAAGTGATTG AACTCCAAGA CATCATAGAT 
AAGCAAAACA AAGAGCAGAC ACAAATGAAA GAACGCCTCA CTGCTTTGTC CAGCAGAGTA 
ACAGAGCTGG AAGAAGATCT AGACACAGCA AGGAAAGACT TAATAAAATC TGAAGAAATG 
AACACAAAGT TACAGAGGGA TGTACGAGAG ACTCTGGCCC AGAAGGAGGA CATGGAGGAG 
AGAATCACCA CTCTGGAGAA ACGCTACCTC GCTGCACAAC GTGAAGCTAC ATCCGTGCAT 
GACCTCAATG ATAAACTTGA AAATGAAATT GCCACTAAAG ACTCCATGCA CCGACAGAGT 
GAAGATAAGA ACAGGCAACT GCAAGAGCGA CTGGAACTGG CTGAGCAAAA GCTGCAGCAG 
ACCCTGAGAA AAGCTGAGAC TTTGCCAGAG GTGGAGGCTG AGCTGGCACA GAGAGTTGCA 
GCACTTACAA AGTCTGAACT TTTGTCTCCT GGGAGCTCTG CTGCTAAAGA TGCTAAAGTT 
TTGGAACTTG CCACCAAGCT TAGGAAGGCT GAGGAGAGAC ATGGCAATAT CGAGGAGCGA 
CTGAGACAGA TGGAGGCACA GCTTGAAGAG AAGAATCAAG AACTGCAAAG GGCTAGACAG 
AGAGAGAAGA TGAATGAAGA GCATAATAAA CGTTTGTCAG AAACTGTTGA TAAGCTCCTA 
TCTGAATCCA ATGAAAGGCT CCAACTTCAT CTCAAGGAGA GGATGGCTGC CCTGGAAGAT 
AAGAATTCAC TTCTTCGAGA AATTGAAAAT GCAAAGAAAC AAATAGAAGA ACTTCAGCAT 
GAAAAGGATC AACTTGTATT AAATGTGGAT GCATTAAGGG CTGAAAATGA CCAAGTGAGG 
CTCAGAGCCA CATCACTTCA TCATAGCAGA CCAGATTTCA GATTCCCTGT GCCACCCTCC 
TCTGTGGGGG ACACTCACAC AGACTCCTAC AGCACCTCCT CTGTGCTGAG GCGGCCCCAG 
AAGGGGCGTC TGGCTGCTCT CAGGGACGAG CCTTCAAAGG TTCAGACCCT GAATGAGCAG 
GATTGGGAGC GGGCCCAGCA GGCCAGTGTT TTGGCAAATG TGGCACAAGC ATTTGAGAGT 
GATGTGGATG TGTCTGATGT GGAGGATGAC AGGGAGACCA TTTTCAGCTC AGTTGATCTG 
CTGTCACCAA GTGGTCAGGC TGATGCTCAG ACTTTGGCCA TAATGCTGCA GGAACAGTTG 
GATGCAATCA ACAAAGAGAT CAGACTGATC CAGGAGGAGA AGGAGAACAC GGAGCAGCGC 
GCGGAGGAGA TTGAGAGCCG TGTGGGCAGC GGCAGCCTGG AGGCGCACGC CCGGTTCCGC 
TCGCTGGGCT CCATCCCCCC CGCCTACGGT GGGGCCCTGG CCGGCTCCTC CCCGCCCGGC 
AGCGGCCGCT CCACACCCCG GCGCATCCCA CACAGCCCGG CCAGGGAAGT GGACAGGCTG 
GGCATCATGA CATTGCCTAG TGATTTAAGG AAACACCGTA GAAAGTCTCC AGCTTCCAGG 
GAAGAGGTAC GAGATGACAA AACCACCATA AAATGTGAGA CATCACCACC TTCATCACCT 
CGGTCTTTGC GTCTGGACAG AGTCCAGAAA GGAGCTCTGC ACACAGTGAG CCACGAAGAT 
ATCAGGGACA TTAGGAATTC AACAGGTTCA CAAGATGGGC AAACAAGTAA TCCTAGTAGC 
AGTAACAGCA GCCAAGATTC CCTTCACAAA GCCCCCAAAA AAAAGGGAAT CAAATCCTCT 
ATTGGCCGCC TGTTTGGCAA GAAGGAAAAG GGACGACCTG GACAAGGAAG CAAGGAATCT 
CTAGGACAAG GTGGCATGGG AGAAGCAGAT GGTTCCTCTC AGGATGCATT AGGTCTCAGC 
AAACTTGGAG GTCAGGCAGA GAAAAACAGG AAAATGCAGA AGAAGCACGA GTTGCTGGAG 
GAAGCCAGAA GACAGGGTTT GCCTTTTGCA CAGTGGGATG GGCCCACTGT GGTGGTGTGG 
CTGGAGCTGT GGGTGGGGAT GCCAGCCTGG TACGTGGCTG CCTGCCGAGC CAATGTGAAA 
AGCGGTGCTA TCATGTCAGC CCTGTCTGAC ACAGAAATCC AGCGGGAAAT CGGCATCAGC 
AATCCCCTGC ACAGGCTCAA GCTCCGCCTG GCCATCCAGG AAATCATGTC CCTAACCAGC 
CCCTCTGCCC CTCCCACCTC AAGGACGACC ACGGGAAATG TCTGGGTAAC ACATGAAGAG 
ATGGAAAATC TTACAGCCAC ACAACAAACG GAAGATGAGG AGGGAAGCTG GGCTCAGACA 
TTAGCCTATG GTGACATGAA CCATGAGTGG ATTGGCAATG AGTGGCTGCC AAGCCTGGGG 
CTCCCTCAGT ACCGCAGCTA CTTCATGGAA TGTCTGGTGG ATGCTCGGAT GCTGGATCAC 
TTAACTAAAA AAGACCTGCG TGGGCAGCTG AAAATGGTGG ACAGCTTTCA CAGAAACAGT 
TTTCAGTGTG GAATTATGTG CCTCCGAAGA CTGAATTACG ATCGAAAAGA GCTAGAAAGA 
AAAAGAGAAG AAAGCCAGAA TGAAATTAAG GATGTCCTTG TGTGGAGCAA TGAGAGGATG 
ATCCACTGGG TCATGTCCAT TGGCCTTAAG GAATATGCGA ACAACCTGCT CGAGAGCGGA 
GTTCACGGTG CACTTGTGGC CTTAGATGAA TCCTTTGATT ACAATGCATT AGCTCTCCTG 
CTGCAAATCC CCACTCAAAA CACACAGGCT CGTGCTGTTC TGGAGAGGGA ATTCAATAAC 
CTTCTTGCTA TGGGCACTGA CAGAAGACTT GATGAAGATG ATGATAAGAG CTTTAGGAGA 
GCACCTTCGT GGAGAAAGAA GTTTAGACCA AAGGACATAA GAGGTTTAGC TACTGGATCA 
GCAGAGACTC TTCCTGCAAA TTTCAGAGTT ACAACCTCAA TGTCTTCACC CTCTATGCAG 
CCAAAGAAGA TGCAGATTGA TGGCAGTGTA TCAGGAACAC AAAGATTGGA TTCTGCTACA 
GTAAGGACCT ACTCCTGTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015486854.1
CDS	914..4714
Translation	MMCEVMPTISEAEIPAGGNGGHGSGSPLQSDADSHFEQLMVSMLEERDRLLETLRETQETLALTQGKLHEVGHERDSLQRQLNTALPQEFAALTKELNVCREQLLEREEEIAELKAERNNTRLLLEHLECLVSRHERSLRMTVVKRQAQSPAGVSSEVEVLKALKSLFEHHKALDEKVRERLRVALERCSLLEEELGTTHKELMILKEQNNQKKTLPDGMLDINHEQENTPTTNGKRSSDGSLCHDENLAKVIELQDIIDKQNKEQTQMKERLTALSSRVTELEEDLDTARKDLIKSEEMNTKLQRDVRETLAQKEDMEERITTLEKRYLAAQREATSVHDLNDKLENEIATKDSMHRQSEDKNRQLQERLELAEQKLQQTLRKAETLPEVEAELAQRVAALTKSELLSPGSSAAKDAKVLELATKLRKAEERHGNIEERLRQMEAQLEEKNQELQRARQREKMNEEHNKRLSETVDKLLSESNERLQLHLKERMAALEDKNSLLREIENAKKQIEELQHEKDQLVLNVDALRAENDQVRLRATSLHHSRPDFRFPVPPSSVGDTHTDSYSTSSVLRRPQKGRLAALRDEPSKVQTLNEQDWERAQQASVLANVAQAFESDVDVSDVEDDRETIFSSVDLLSPSGQADAQTLAIMLQEQLDAINKEIRLIQEEKENTEQRAEEIESRVGSGSLEAHARFRSLGSIPPAYGGALAGSSPPGSGRSTPRRIPHSPAREVDRLGIMTLPSDLRKHRRKSPASREEVRDDKTTIKCETSPPSSPRSLRLDRVQKGALHTVSHEDIRDIRNSTGSQDGQTSNPSSSNSSQDSLHKAPKKKGIKSSIGRLFGKKEKGRPGQGSKESLGQGGMGEADGSSQDALGLSKLGGQAEKNRKMQKKHELLEEARRQGLPFAQWDGPTVVVWLELWVGMPAWYVAACRANVKSGAIMSALSDTEIQREIGISNPLHRLKLRLAIQEIMSLTSPSAPPTSRTTTGNVWVTHEEMENLTATQQTEDEEGSWAQTLAYGDMNHEWIGNEWLPSLGLPQYRSYFMECLVDARMLDHLTKKDLRGQLKMVDSFHRNSFQCGIMCLRRLNYDRKELERKREESQNEIKDVLVWSNERMIHWVMSIGLKEYANNLLESGVHGALVALDESFDYNALALLLQIPTQNTQARAVLEREFNNLLAMGTDRRLDEDDDKSFRRAPSWRKKFRPKDIRGLATGSAETLPANFRVTTSMSSPSMQPKKMQIDGSVSGTQRLDSATVRTYSC

Target ORF information:

RefSeq Version	XM_015631368.2
Organism	Parus major(Great Tit)
Definition	Parus major PTPRF interacting protein alpha 1 (PPFIA1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015631368.2

ORF Insert Sequence:

ATGATGTGTG AGGTGATGCC AACCATCAGT GAGGCCGAGA TTCCCGCCGG GGGAAATGGA 
GGCCACGGTT CAGGTTCCCC GTTGCAATCA GATGCTGATT CCCACTTCGA GCAGTTAATG 
GTGTCCATGC TGGAAGAAAG GGATCGCCTC CTAGAAACGC TGCGAGAAAC TCAAGAGACG 
CTTGCCCTCA CCCAGGGCAA GCTGCATGAG GTTGGCCATG AGAGGGATTC CTTGCAGAGG 
CAGCTCAATA CTGCACTTCC ACAGGAATTT GCAGCACTCA CCAAAGAGCT GAATGTATGC 
AGGGAGCAGC TGCTTGAAAG GGAAGAAGAA ATTGCTGAGC TGAAAGCAGA AAGAAATAAT 
ACGAGGCTAT TACTGGAACA TTTGGAGTGT CTTGTCTCCA GGCATGAGCG ATCCCTCAGG 
ATGACTGTTG TGAAGAGGCA AGCTCAGTCT CCAGCAGGAG TTTCTAGTGA AGTAGAAGTT 
CTCAAAGCAC TAAAATCTTT ATTTGAGCAC CACAAAGCCC TTGATGAAAA GGTAAGGGAG 
AGATTACGAG TAGCACTTGA AAGATGTAGC TTATTGGAAG AAGAACTAGG TACTACACAT 
AAAGAGTTAA TGATTCTGAA AGAGCAAAAT AATCAGAAGA AAACACTTCC AGATGGGATG 
CTGGATATAA ATCATGAACA AGAAAATACA CCAACTACAA ATGGGAAGAG ATCCTCTGAT 
GGTTCATTAT GCCATGATGA AAACCTTGCT AAAGTGATTG AACTCCAAGA CATCATAGAT 
AAGCAAAACA AAGAGCAGAC ACAAATGAAA GAACGCCTCA CTGCTTTGTC CAGCAGAGTA 
ACAGAGCTGG AAGAAGATCT AGACACAGCA AGGAAAGACT TAATAAAATC TGAAGAAATG 
AACACAAAGT TACAGAGGGA TGTACGAGAG ACTCTGGCCC AGAAGGAGGA CATGGAGGAG 
AGAATCACCA CTCTGGAGAA ACGCTACCTC GCTGCACAAC GTGAAGCTAC ATCCGTGCAT 
GACCTCAATG ATAAACTTGA AAATGAAATT GCCACTAAAG ACTCCATGCA CCGACAGAGT 
GAAGATAAGA ACAGGCAACT GCAAGAGCGA CTGGAACTGG CTGAGCAAAA GCTGCAGCAG 
ACCCTGAGAA AAGCTGAGAC TTTGCCAGAG GTGGAGGCTG AGCTGGCACA GAGAGTTGCA 
GCACTTACAA AGTCTGAACT TTTGTCTCCT GGGAGCTCTG CTGCTAAAGA TGCTAAAGTT 
TTGGAACTTG CCACCAAGCT TAGGAAGGCT GAGGAGAGAC ATGGCAATAT CGAGGAGCGA 
CTGAGACAGA TGGAGGCACA GCTTGAAGAG AAGAATCAAG AACTGCAAAG GGCTAGACAG 
AGAGAGAAGA TGAATGAAGA GCATAATAAA CGTTTGTCAG AAACTGTTGA TAAGCTCCTA 
TCTGAATCCA ATGAAAGGCT CCAACTTCAT CTCAAGGAGA GGATGGCTGC CCTGGAAGAT 
AAGAATTCAC TTCTTCGAGA AATTGAAAAT GCAAAGAAAC AAATAGAAGA ACTTCAGCAT 
GAAAAGGATC AACTTGTATT AAATGTGGAT GCATTAAGGG CTGAAAATGA CCAAGTGAGG 
CTCAGAGCCA CATCACTTCA TCATAGCAGA CCAGATTTCA GATTCCCTGT GCCACCCTCC 
TCTGTGGGGG ACACTCACAC AGACTCCTAC AGCACCTCCT CTGTGCTGAG GCGGCCCCAG 
AAGGGGCGTC TGGCTGCTCT CAGGGACGAG CCTTCAAAGG TTCAGACCCT GAATGAGCAG 
GATTGGGAGC GGGCCCAGCA GGCCAGTGTT TTGGCAAATG TGGCACAAGC ATTTGAGAGT 
GATGTGGATG TGTCTGATGT GGAGGATGAC AGGGAGACCA TTTTCAGCTC AGTTGATCTG 
CTGTCACCAA GTGGTCAGGC TGATGCTCAG ACTTTGGCCA TAATGCTGCA GGAACAGTTG 
GATGCAATCA ACAAAGAGAT CAGACTGATC CAGGAGGAGA AGGAGAACAC GGAGCAGCGC 
GCGGAGGAGA TTGAGAGCCG TGTGGGCAGC GGCAGCCTGG AGGCGCACGC CCGGTTCCGC 
TCGCTGGGCT CCATCCCCCC CGCCTACGGT GGGGCCCTGG CCGGCTCCTC CCCGCCCGGC 
AGCGGCCGCT CCACACCCCG GCGCATCCCA CACAGCCCGG CCAGGGAAGT GGACAGGCTG 
GGCATCATGA CATTGCCTAG TGATTTAAGG AAACACCGTA GAAAGTCTCC AGCTTCCAGG 
GAAGAGGTAC GAGATGACAA AACCACCATA AAATGTGAGA CATCACCACC TTCATCACCT 
CGGTCTTTGC GTCTGGACAG AGTCCAGAAA GGAGCTCTGC ACACAGTGAG CCACGAAGAT 
ATCAGGGACA TTAGGAATTC AACAGGTTCA CAAGATGGGC AAACAAGTAA TCCTAGTAGC 
AGTAACAGCA GCCAAGATTC CCTTCACAAA GCCCCCAAAA AAAAGGGAAT CAAATCCTCT 
ATTGGCCGCC TGTTTGGCAA GAAGGAAAAG GGACGACCTG GACAAGGAAG CAAGGAATCT 
CTAGGACAAG GTGGCATGGG AGAAGCAGAT GGTTCCTCTC AGGATGCATT AGGTCTCAGC 
AAACTTGGAG GTCAGGCAGA GAAAAACAGG AAAATGCAGA AGAAGCACGA GTTGCTGGAG 
GAAGCCAGAA GACAGGGTTT GCCTTTTGCA CAGTGGGATG GGCCCACTGT GGTGGTGTGG 
CTGGAGCTGT GGGTGGGGAT GCCAGCCTGG TACGTGGCTG CCTGCCGAGC CAATGTGAAA 
AGCGGTGCTA TCATGTCAGC CCTGTCTGAC ACAGAAATCC AGCGGGAAAT CGGCATCAGC 
AATCCCCTGC ACAGGCTCAA GCTCCGCCTG GCCATCCAGG AAATCATGTC CCTAACCAGC 
CCCTCTGCCC CTCCCACCTC AAGGACGACC ACGGGAAATG TCTGGGTAAC ACATGAAGAG 
ATGGAAAATC TTACAGCCAC ACAACAAACG GAAGATGAGG AGGGAAGCTG GGCTCAGACA 
TTAGCCTATG GTGACATGAA CCATGAGTGG ATTGGCAATG AGTGGCTGCC AAGCCTGGGG 
CTCCCTCAGT ACCGCAGCTA CTTCATGGAA TGTCTGGTGG ATGCTCGGAT GCTGGATCAC 
TTAACTAAAA AAGACCTGCG TGGGCAGCTG AAAATGGTGG ACAGCTTTCA CAGAAACAGT 
TTTCAGTGTG GAATTATGTG CCTCCGAAGA CTGAATTACG ATCGAAAAGA GCTAGAAAGA 
AAAAGAGAAG AAAGCCAGAA TGAAATTAAG GATGTCCTTG TGTGGAGCAA TGAGAGGATG 
ATCCACTGGG TCATGTCCAT TGGCCTTAAG GAATATGCGA ACAACCTGCT CGAGAGCGGA 
GTTCACGGTG CACTTGTGGC CTTAGATGAA TCCTTTGATT ACAATGCATT AGCTCTCCTG 
CTGCAAATCC CCACTCAAAA CACACAGGCT CGTGCTGTTC TGGAGAGGGA ATTCAATAAC 
CTTCTTGCTA TGGGCACTGA CAGAAGACTT GATGAAGATG ATGATAAGAG CTTTAGGAGA 
GCACCTTCGT GGAGAAAGAA GTTTAGACCA AAGGACATAA GAGGTTTAGC TACTGGATCA 
GCAGAGACTC TTCCTGCAAA TTTCAGAGTT ACAACCTCAA TGTCTTCACC CTCTATGCAG 
CCAAAGAAGA TGCAGATTGA TGGCAGTGTA TCAGGAACAC AAAGATTGGA TTCTGCTACA 
GTAAGGACCT ACTCCTGTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PPFIA1 ( XM_015631369.2 ) cDNA ORF clone, Parus major(Great Tit) -> XP_015486855.1 Parus major PTPRF interacting protein alpha 1 (PPFIA1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OPa179669
Clone ID Related Accession (Same CDS sequence)	XM_015631369.2
Accession Version	XM_015631369.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3798bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-11-13
Organism	Parus major(Great Tit)
Product	liprin-alpha-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_031774.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Nov 4, 2016 this sequence version replaced XM_015631369.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Parus major Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTGTG AGGTGATGCC AACCATCAGT GAGGCCGAGA TTCCCGCCGG GGGAAATGGA 
GGCCACGGTT CAGGTTCCCC GTTGCAATCA GATGCTGATT CCCACTTCGA GCAGTTAATG 
GTGTCCATGC TGGAAGAAAG GGATCGCCTC CTAGAAACGC TGCGAGAAAC TCAAGAGACG 
CTTGCCCTCA CCCAGGGCAA GCTGCATGAG GTTGGCCATG AGAGGGATTC CTTGCAGAGG 
CAGCTCAATA CTGCACTTCC ACAGGAATTT GCAGCACTCA CCAAAGAGCT GAATGTATGC 
AGGGAGCAGC TGCTTGAAAG GGAAGAAGAA ATTGCTGAGC TGAAAGCAGA AAGAAATAAT 
ACGAGGCTAT TACTGGAACA TTTGGAGTGT CTTGTCTCCA GGCATGAGCG ATCCCTCAGG 
ATGACTGTTG TGAAGAGGCA AGCTCAGTCT CCAGCAGGAG TTTCTAGTGA AGTAGAAGTT 
CTCAAAGCAC TAAAATCTTT ATTTGAGCAC CACAAAGCCC TTGATGAAAA GGTAAGGGAG 
AGATTACGAG TAGCACTTGA AAGATGTAGC TTATTGGAAG AAGAACTAGG TACTACACAT 
AAAGAGTTAA TGATTCTGAA AGAGCAAAAT AATCAGAAGA AAACACTTCC AGATGGGATG 
CTGGATATAA ATCATGAACA AGAAAATACA CCAACTACAA ATGGGAAGAG ATCCTCTGAT 
GGTTCATTAT GCCATGATGA AAACCTTGCT AAAGTGATTG AACTCCAAGA CATCATAGAT 
AAGCAAAACA AAGAGCAGAC ACAAATGAAA GAACGCCTCA CTGCTTTGTC CAGCAGAGTA 
ACAGAGCTGG AAGAAGATCT AGACACAGCA AGGAAAGACT TAATAAAATC TGAAGAAATG 
AACACAAAGT TACAGAGGGA TGTACGAGAG ACTCTGGCCC AGAAGGAGGA CATGGAGGAG 
AGAATCACCA CTCTGGAGAA ACGCTACCTC GCTGCACAAC GTGAAGCTAC ATCCGTGCAT 
GACCTCAATG ATAAACTTGA AAATGAAATT GCCACTAAAG ACTCCATGCA CCGACAGAGT 
GAAGATAAGA ACAGGCAACT GCAAGAGCGA CTGGAACTGG CTGAGCAAAA GCTGCAGCAG 
ACCCTGAGAA AAGCTGAGAC TTTGCCAGAG GTGGAGGCTG AGCTGGCACA GAGAGTTGCA 
GCACTTACAA AGTCTGAACT TTTGTCTCCT GGGAGCTCTG CTGCTAAAGA TGCTAAAGTT 
TTGGAACTTG CCACCAAGCT TAGGAAGGCT GAGGAGAGAC ATGGCAATAT CGAGGAGCGA 
CTGAGACAGA TGGAGGCACA GCTTGAAGAG AAGAATCAAG AACTGCAAAG GGCTAGACAG 
AGAGAGAAGA TGAATGAAGA GCATAATAAA CGTTTGTCAG AAACTGTTGA TAAGCTCCTA 
TCTGAATCCA ATGAAAGGCT CCAACTTCAT CTCAAGGAGA GGATGGCTGC CCTGGAAGAT 
AAGAATTCAC TTCTTCGAGA AATTGAAAAT GCAAAGAAAC AAATAGAAGA ACTTCAGCAT 
GAAAAGGATC AACTTGTATT AAATGTGGAT GCATTAAGGG CTGAAAATGA CCAAGTGAGG 
CTCAGAGCCA CATCACTTCA TCATAGACCA GATTTCAGAT TCCCTGTGCC ACCCTCCTCT 
GTGGGGGACA CTCACACAGA CTCCTACAGC ACCTCCTCTG TGCTGAGGCG GCCCCAGAAG 
GGGCGTCTGG CTGCTCTCAG GGACGAGCCT TCAAAGGTTC AGACCCTGAA TGAGCAGGAT 
TGGGAGCGGG CCCAGCAGGC CAGTGTTTTG GCAAATGTGG CACAAGCATT TGAGAGTGAT 
GTGGATGTGT CTGATGTGGA GGATGACAGG GAGACCATTT TCAGCTCAGT TGATCTGCTG 
TCACCAAGTG GTCAGGCTGA TGCTCAGACT TTGGCCATAA TGCTGCAGGA ACAGTTGGAT 
GCAATCAACA AAGAGATCAG ACTGATCCAG GAGGAGAAGG AGAACACGGA GCAGCGCGCG 
GAGGAGATTG AGAGCCGTGT GGGCAGCGGC AGCCTGGAGG CGCACGCCCG GTTCCGCTCG 
CTGGGCTCCA TCCCCCCCGC CTACGGTGGG GCCCTGGCCG GCTCCTCCCC GCCCGGCAGC 
GGCCGCTCCA CACCCCGGCG CATCCCACAC AGCCCGGCCA GGGAAGTGGA CAGGCTGGGC 
ATCATGACAT TGCCTAGTGA TTTAAGGAAA CACCGTAGAA AGTCTCCAGC TTCCAGGGAA 
GAGGTACGAG ATGACAAAAC CACCATAAAA TGTGAGACAT CACCACCTTC ATCACCTCGG 
TCTTTGCGTC TGGACAGAGT CCAGAAAGGA GCTCTGCACA CAGTGAGCCA CGAAGATATC 
AGGGACATTA GGAATTCAAC AGGTTCACAA GATGGGCAAA CAAGTAATCC TAGTAGCAGT 
AACAGCAGCC AAGATTCCCT TCACAAAGCC CCCAAAAAAA AGGGAATCAA ATCCTCTATT 
GGCCGCCTGT TTGGCAAGAA GGAAAAGGGA CGACCTGGAC AAGGAAGCAA GGAATCTCTA 
GGACAAGGTG GCATGGGAGA AGCAGATGGT TCCTCTCAGG ATGCATTAGG TCTCAGCAAA 
CTTGGAGGTC AGGCAGAGAA AAACAGGAAA ATGCAGAAGA AGCACGAGTT GCTGGAGGAA 
GCCAGAAGAC AGGGTTTGCC TTTTGCACAG TGGGATGGGC CCACTGTGGT GGTGTGGCTG 
GAGCTGTGGG TGGGGATGCC AGCCTGGTAC GTGGCTGCCT GCCGAGCCAA TGTGAAAAGC 
GGTGCTATCA TGTCAGCCCT GTCTGACACA GAAATCCAGC GGGAAATCGG CATCAGCAAT 
CCCCTGCACA GGCTCAAGCT CCGCCTGGCC ATCCAGGAAA TCATGTCCCT AACCAGCCCC 
TCTGCCCCTC CCACCTCAAG GACGACCACG GGAAATGTCT GGGTAACACA TGAAGAGATG 
GAAAATCTTA CAGCCACACA ACAAACGGAA GATGAGGAGG GAAGCTGGGC TCAGACATTA 
GCCTATGGTG ACATGAACCA TGAGTGGATT GGCAATGAGT GGCTGCCAAG CCTGGGGCTC 
CCTCAGTACC GCAGCTACTT CATGGAATGT CTGGTGGATG CTCGGATGCT GGATCACTTA 
ACTAAAAAAG ACCTGCGTGG GCAGCTGAAA ATGGTGGACA GCTTTCACAG AAACAGTTTT 
CAGTGTGGAA TTATGTGCCT CCGAAGACTG AATTACGATC GAAAAGAGCT AGAAAGAAAA 
AGAGAAGAAA GCCAGAATGA AATTAAGGAT GTCCTTGTGT GGAGCAATGA GAGGATGATC 
CACTGGGTCA TGTCCATTGG CCTTAAGGAA TATGCGAACA ACCTGCTCGA GAGCGGAGTT 
CACGGTGCAC TTGTGGCCTT AGATGAATCC TTTGATTACA ATGCATTAGC TCTCCTGCTG 
CAAATCCCCA CTCAAAACAC ACAGGCTCGT GCTGTTCTGG AGAGGGAATT CAATAACCTT 
CTTGCTATGG GCACTGACAG AAGACTTGAT GAAGATGATG ATAAGAGCTT TAGGAGAGCA 
CCTTCGTGGA GAAAGAAGTT TAGACCAAAG GACATAAGAG GTTTAGCTAC TGGATCAGCA 
GAGACTCTTC CTGCAAATTT CAGAGTTACA ACCTCAATGT CTTCACCCTC TATGCAGCCA 
AAGAAGATGC AGATTGATGG CAGTGTATCA GGAACACAAA GATTGGATTC TGCTACAGTA 
AGGACCTACT CCTGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015486855.1
CDS	914..4711
Translation	MMCEVMPTISEAEIPAGGNGGHGSGSPLQSDADSHFEQLMVSMLEERDRLLETLRETQETLALTQGKLHEVGHERDSLQRQLNTALPQEFAALTKELNVCREQLLEREEEIAELKAERNNTRLLLEHLECLVSRHERSLRMTVVKRQAQSPAGVSSEVEVLKALKSLFEHHKALDEKVRERLRVALERCSLLEEELGTTHKELMILKEQNNQKKTLPDGMLDINHEQENTPTTNGKRSSDGSLCHDENLAKVIELQDIIDKQNKEQTQMKERLTALSSRVTELEEDLDTARKDLIKSEEMNTKLQRDVRETLAQKEDMEERITTLEKRYLAAQREATSVHDLNDKLENEIATKDSMHRQSEDKNRQLQERLELAEQKLQQTLRKAETLPEVEAELAQRVAALTKSELLSPGSSAAKDAKVLELATKLRKAEERHGNIEERLRQMEAQLEEKNQELQRARQREKMNEEHNKRLSETVDKLLSESNERLQLHLKERMAALEDKNSLLREIENAKKQIEELQHEKDQLVLNVDALRAENDQVRLRATSLHHRPDFRFPVPPSSVGDTHTDSYSTSSVLRRPQKGRLAALRDEPSKVQTLNEQDWERAQQASVLANVAQAFESDVDVSDVEDDRETIFSSVDLLSPSGQADAQTLAIMLQEQLDAINKEIRLIQEEKENTEQRAEEIESRVGSGSLEAHARFRSLGSIPPAYGGALAGSSPPGSGRSTPRRIPHSPAREVDRLGIMTLPSDLRKHRRKSPASREEVRDDKTTIKCETSPPSSPRSLRLDRVQKGALHTVSHEDIRDIRNSTGSQDGQTSNPSSSNSSQDSLHKAPKKKGIKSSIGRLFGKKEKGRPGQGSKESLGQGGMGEADGSSQDALGLSKLGGQAEKNRKMQKKHELLEEARRQGLPFAQWDGPTVVVWLELWVGMPAWYVAACRANVKSGAIMSALSDTEIQREIGISNPLHRLKLRLAIQEIMSLTSPSAPPTSRTTTGNVWVTHEEMENLTATQQTEDEEGSWAQTLAYGDMNHEWIGNEWLPSLGLPQYRSYFMECLVDARMLDHLTKKDLRGQLKMVDSFHRNSFQCGIMCLRRLNYDRKELERKREESQNEIKDVLVWSNERMIHWVMSIGLKEYANNLLESGVHGALVALDESFDYNALALLLQIPTQNTQARAVLEREFNNLLAMGTDRRLDEDDDKSFRRAPSWRKKFRPKDIRGLATGSAETLPANFRVTTSMSSPSMQPKKMQIDGSVSGTQRLDSATVRTYSC

Target ORF information:

RefSeq Version	XM_015631369.2
Organism	Parus major(Great Tit)
Definition	Parus major PTPRF interacting protein alpha 1 (PPFIA1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015631369.2

ORF Insert Sequence:

ATGATGTGTG AGGTGATGCC AACCATCAGT GAGGCCGAGA TTCCCGCCGG GGGAAATGGA 
GGCCACGGTT CAGGTTCCCC GTTGCAATCA GATGCTGATT CCCACTTCGA GCAGTTAATG 
GTGTCCATGC TGGAAGAAAG GGATCGCCTC CTAGAAACGC TGCGAGAAAC TCAAGAGACG 
CTTGCCCTCA CCCAGGGCAA GCTGCATGAG GTTGGCCATG AGAGGGATTC CTTGCAGAGG 
CAGCTCAATA CTGCACTTCC ACAGGAATTT GCAGCACTCA CCAAAGAGCT GAATGTATGC 
AGGGAGCAGC TGCTTGAAAG GGAAGAAGAA ATTGCTGAGC TGAAAGCAGA AAGAAATAAT 
ACGAGGCTAT TACTGGAACA TTTGGAGTGT CTTGTCTCCA GGCATGAGCG ATCCCTCAGG 
ATGACTGTTG TGAAGAGGCA AGCTCAGTCT CCAGCAGGAG TTTCTAGTGA AGTAGAAGTT 
CTCAAAGCAC TAAAATCTTT ATTTGAGCAC CACAAAGCCC TTGATGAAAA GGTAAGGGAG 
AGATTACGAG TAGCACTTGA AAGATGTAGC TTATTGGAAG AAGAACTAGG TACTACACAT 
AAAGAGTTAA TGATTCTGAA AGAGCAAAAT AATCAGAAGA AAACACTTCC AGATGGGATG 
CTGGATATAA ATCATGAACA AGAAAATACA CCAACTACAA ATGGGAAGAG ATCCTCTGAT 
GGTTCATTAT GCCATGATGA AAACCTTGCT AAAGTGATTG AACTCCAAGA CATCATAGAT 
AAGCAAAACA AAGAGCAGAC ACAAATGAAA GAACGCCTCA CTGCTTTGTC CAGCAGAGTA 
ACAGAGCTGG AAGAAGATCT AGACACAGCA AGGAAAGACT TAATAAAATC TGAAGAAATG 
AACACAAAGT TACAGAGGGA TGTACGAGAG ACTCTGGCCC AGAAGGAGGA CATGGAGGAG 
AGAATCACCA CTCTGGAGAA ACGCTACCTC GCTGCACAAC GTGAAGCTAC ATCCGTGCAT 
GACCTCAATG ATAAACTTGA AAATGAAATT GCCACTAAAG ACTCCATGCA CCGACAGAGT 
GAAGATAAGA ACAGGCAACT GCAAGAGCGA CTGGAACTGG CTGAGCAAAA GCTGCAGCAG 
ACCCTGAGAA AAGCTGAGAC TTTGCCAGAG GTGGAGGCTG AGCTGGCACA GAGAGTTGCA 
GCACTTACAA AGTCTGAACT TTTGTCTCCT GGGAGCTCTG CTGCTAAAGA TGCTAAAGTT 
TTGGAACTTG CCACCAAGCT TAGGAAGGCT GAGGAGAGAC ATGGCAATAT CGAGGAGCGA 
CTGAGACAGA TGGAGGCACA GCTTGAAGAG AAGAATCAAG AACTGCAAAG GGCTAGACAG 
AGAGAGAAGA TGAATGAAGA GCATAATAAA CGTTTGTCAG AAACTGTTGA TAAGCTCCTA 
TCTGAATCCA ATGAAAGGCT CCAACTTCAT CTCAAGGAGA GGATGGCTGC CCTGGAAGAT 
AAGAATTCAC TTCTTCGAGA AATTGAAAAT GCAAAGAAAC AAATAGAAGA ACTTCAGCAT 
GAAAAGGATC AACTTGTATT AAATGTGGAT GCATTAAGGG CTGAAAATGA CCAAGTGAGG 
CTCAGAGCCA CATCACTTCA TCATAGACCA GATTTCAGAT TCCCTGTGCC ACCCTCCTCT 
GTGGGGGACA CTCACACAGA CTCCTACAGC ACCTCCTCTG TGCTGAGGCG GCCCCAGAAG 
GGGCGTCTGG CTGCTCTCAG GGACGAGCCT TCAAAGGTTC AGACCCTGAA TGAGCAGGAT 
TGGGAGCGGG CCCAGCAGGC CAGTGTTTTG GCAAATGTGG CACAAGCATT TGAGAGTGAT 
GTGGATGTGT CTGATGTGGA GGATGACAGG GAGACCATTT TCAGCTCAGT TGATCTGCTG 
TCACCAAGTG GTCAGGCTGA TGCTCAGACT TTGGCCATAA TGCTGCAGGA ACAGTTGGAT 
GCAATCAACA AAGAGATCAG ACTGATCCAG GAGGAGAAGG AGAACACGGA GCAGCGCGCG 
GAGGAGATTG AGAGCCGTGT GGGCAGCGGC AGCCTGGAGG CGCACGCCCG GTTCCGCTCG 
CTGGGCTCCA TCCCCCCCGC CTACGGTGGG GCCCTGGCCG GCTCCTCCCC GCCCGGCAGC 
GGCCGCTCCA CACCCCGGCG CATCCCACAC AGCCCGGCCA GGGAAGTGGA CAGGCTGGGC 
ATCATGACAT TGCCTAGTGA TTTAAGGAAA CACCGTAGAA AGTCTCCAGC TTCCAGGGAA 
GAGGTACGAG ATGACAAAAC CACCATAAAA TGTGAGACAT CACCACCTTC ATCACCTCGG 
TCTTTGCGTC TGGACAGAGT CCAGAAAGGA GCTCTGCACA CAGTGAGCCA CGAAGATATC 
AGGGACATTA GGAATTCAAC AGGTTCACAA GATGGGCAAA CAAGTAATCC TAGTAGCAGT 
AACAGCAGCC AAGATTCCCT TCACAAAGCC CCCAAAAAAA AGGGAATCAA ATCCTCTATT 
GGCCGCCTGT TTGGCAAGAA GGAAAAGGGA CGACCTGGAC AAGGAAGCAA GGAATCTCTA 
GGACAAGGTG GCATGGGAGA AGCAGATGGT TCCTCTCAGG ATGCATTAGG TCTCAGCAAA 
CTTGGAGGTC AGGCAGAGAA AAACAGGAAA ATGCAGAAGA AGCACGAGTT GCTGGAGGAA 
GCCAGAAGAC AGGGTTTGCC TTTTGCACAG TGGGATGGGC CCACTGTGGT GGTGTGGCTG 
GAGCTGTGGG TGGGGATGCC AGCCTGGTAC GTGGCTGCCT GCCGAGCCAA TGTGAAAAGC 
GGTGCTATCA TGTCAGCCCT GTCTGACACA GAAATCCAGC GGGAAATCGG CATCAGCAAT 
CCCCTGCACA GGCTCAAGCT CCGCCTGGCC ATCCAGGAAA TCATGTCCCT AACCAGCCCC 
TCTGCCCCTC CCACCTCAAG GACGACCACG GGAAATGTCT GGGTAACACA TGAAGAGATG 
GAAAATCTTA CAGCCACACA ACAAACGGAA GATGAGGAGG GAAGCTGGGC TCAGACATTA 
GCCTATGGTG ACATGAACCA TGAGTGGATT GGCAATGAGT GGCTGCCAAG CCTGGGGCTC 
CCTCAGTACC GCAGCTACTT CATGGAATGT CTGGTGGATG CTCGGATGCT GGATCACTTA 
ACTAAAAAAG ACCTGCGTGG GCAGCTGAAA ATGGTGGACA GCTTTCACAG AAACAGTTTT 
CAGTGTGGAA TTATGTGCCT CCGAAGACTG AATTACGATC GAAAAGAGCT AGAAAGAAAA 
AGAGAAGAAA GCCAGAATGA AATTAAGGAT GTCCTTGTGT GGAGCAATGA GAGGATGATC 
CACTGGGTCA TGTCCATTGG CCTTAAGGAA TATGCGAACA ACCTGCTCGA GAGCGGAGTT 
CACGGTGCAC TTGTGGCCTT AGATGAATCC TTTGATTACA ATGCATTAGC TCTCCTGCTG 
CAAATCCCCA CTCAAAACAC ACAGGCTCGT GCTGTTCTGG AGAGGGAATT CAATAACCTT 
CTTGCTATGG GCACTGACAG AAGACTTGAT GAAGATGATG ATAAGAGCTT TAGGAGAGCA 
CCTTCGTGGA GAAAGAAGTT TAGACCAAAG GACATAAGAG GTTTAGCTAC TGGATCAGCA 
GAGACTCTTC CTGCAAATTT CAGAGTTACA ACCTCAATGT CTTCACCCTC TATGCAGCCA 
AAGAAGATGC AGATTGATGG CAGTGTATCA GGAACACAAA GATTGGATTC TGCTACAGTA 
AGGACCTACT CCTGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PPFIA1 ( XM_015631370.2 ) cDNA ORF clone, Parus major(Great Tit) -> XP_015486856.1 Parus major PTPRF interacting protein alpha 1 (PPFIA1), transcript variant X3, mRNA.

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CloneID	OPa179670
Clone ID Related Accession (Same CDS sequence)	XM_015631370.2
Accession Version	XM_015631370.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3774bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-11-13
Organism	Parus major(Great Tit)
Product	liprin-alpha-1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_031774.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Nov 4, 2016 this sequence version replaced XM_015631370.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Parus major Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTGTG AGGTGATGCC AACCATCAGT GAGGCCGAGA TTCCCGCCGG GGGAAATGGA 
GGCCACGGTT CAGGTTCCCC GTTGCAATCA GATGCTGATT CCCACTTCGA GCAGTTAATG 
GTGTCCATGC TGGAAGAAAG GGATCGCCTC CTAGAAACGC TGCGAGAAAC TCAAGAGACG 
CTTGCCCTCA CCCAGGGCAA GCTGCATGAG GTTGGCCATG AGAGGGATTC CTTGCAGAGG 
CAGCTCAATA CTGCACTTCC ACAGGAATTT GCAGCACTCA CCAAAGAGCT GAATGTATGC 
AGGGAGCAGC TGCTTGAAAG GGAAGAAGAA ATTGCTGAGC TGAAAGCAGA AAGAAATAAT 
ACGAGGCTAT TACTGGAACA TTTGGAGTGT CTTGTCTCCA GGCATGAGCG ATCCCTCAGG 
ATGACTGTTG TGAAGAGGCA AGCTCAGTCT CCAGCAGGAG TTTCTAGTGA AGTAGAAGTT 
CTCAAAGCAC TAAAATCTTT ATTTGAGCAC CACAAAGCCC TTGATGAAAA GGTAAGGGAG 
AGATTACGAG TAGCACTTGA AAGATGTAGC TTATTGGAAG AAGAACTAGG TACTACACAT 
AAAGAGTTAA TGATTCTGAA AGAGCAAAAT AATCAGAAGA AAACACTTCC AGATGGGATG 
CTGGATATAA ATCATGAACA AGAAAATACA CCAACTACAA ATGGGAAGAG ATCCTCTGAT 
GGTTCATTAT GCCATGATGA AAACCTTGCT AAAGTGATTG AACTCCAAGA CATCATAGAT 
AAGCAAAACA AAGAGCAGAC ACAAATGAAA GAACGCCTCA CTGCTTTGTC CAGCAGAGTA 
ACAGAGCTGG AAGAAGATCT AGACACAGCA AGGAAAGACT TAATAAAATC TGAAGAAATG 
AACACAAAGT TACAGAGGGA TGTACGAGAG ACTCTGGCCC AGAAGGAGGA CATGGAGGAG 
AGAATCACCA CTCTGGAGAA ACGCTACCTC GCTGCACAAC GTGAAGCTAC ATCCGTGCAT 
GACCTCAATG ATAAACTTGA AAATGAAATT GCCACTAAAG ACTCCATGCA CCGACAGAGT 
GAAGATAAGA ACAGGCAACT GCAAGAGCGA CTGGAACTGG CTGAGCAAAA GCTGCAGCAG 
ACCCTGAGAA AAGCTGAGAC TTTGCCAGAG GTGGAGGCTG AGCTGGCACA GAGAGTTGCA 
GCACTTACAA AGTCTGAACT TTTGTCTCCT GGGAGCTCTG CTGCTAAAGA TGCTAAAGTT 
TTGGAACTTG CCACCAAGCT TAGGAAGGCT GAGGAGAGAC ATGGCAATAT CGAGGAGCGA 
CTGAGACAGA TGGAGGCACA GCTTGAAGAG AAGAATCAAG AACTGCAAAG GGCTAGACAG 
AGAGAGAAGA TGAATGAAGA GCATAATAAA CGTTTGTCAG AAACTGTTGA TAAGCTCCTA 
TCTGAATCCA ATGAAAGGCT CCAACTTCAT CTCAAGGAGA GGATGGCTGC CCTGGAAGAT 
AAGAATTCAC TTCTTCGAGA AATTGAAAAT GCAAAGAAAC AAATAGAAGA ACTTCAGCAT 
GAAAAGGATC AACTTGTATT AAATGTGGAT GCATTAAGGG CTGAAAATGA CCAAGTGAGG 
CTCAGAGCCA CATCACTTCA TCATAGCAGA CCAGATTTCA GATTCCCTGT GCCACCCTCC 
TCTGTGGGGG ACACTCACAC AGACTCCTAC AGCACCTCCT CTGTGCTGAG GCGGCCCCAG 
AAGGGGCGTC TGGCTGCTCT CAGGGACGAG CCTTCAAAGG TTCAGACCCT GAATGAGCAG 
GATTGGGAGC GGGCCCAGCA GGCCAGTGTT TTGGCAAATG TGGCACAAGC ATTTGAGAGT 
GATGTGGATG TGTCTGATGT GGAGGATGAC AGGGAGACCA TTTTCAGCTC AGTTGATCTG 
CTGTCACCAA GTGGTCAGGC TGATGCTCAG ACTTTGGCCA TAATGCTGCA GGAACAGTTG 
GATGCAATCA ACAAAGAGAT CAGACTGATC CAGGAGGAGA AGGAGAACAC GGAGCAGCGC 
GCGGAGGAGA TTGAGAGCCG TGTGGGCAGC GGCAGCCTGG AGGCGCACGC CCGGTTCCGC 
TCGCTGGGCT CCATCCCCCC CGCCTACGGT GGGGCCCTGG CCGGCTCCTC CCCGCCCGGC 
AGCGGCCGCT CCACACCCCG GCGCATCCCA CACAGCCCGG CCAGGGAAGT GGACAGGCTG 
GGCATCATGA CATTGCCTAG TGATTTAAGG AAACACCGTA GAAAGTCTCC AGCTTCCAGG 
GAAGAGGTAC GAGATGACAA AACCACCATA AAATGTGAGA CATCACCACC TTCATCACCT 
CGGTCTTTGC GTCTGGACAG AGTCCAGAAA GGAGCTCTGC ACACAGTGAG CCACGAAGAT 
ATCAGGGACA TTAGGAATTC AACAGGTTCA CAAGATGGGC AAACAAGTAA TCCTAGTAGC 
AGTAACAGCA GCCAAGATTC CCTTCACAAA GCCCCCAAAA AAAAGGGAAT CAAATCCTCT 
ATTGGCCGCC TGTTTGGCAA GAAGGAAAAG GGACGACCTG GACAAGGAAG CAAGGAATCT 
CTAGGACAAG GTGGCATGGG AGAAGCAGAT GGTTCCTCTC AGGATGCATT AGGTCTCAGC 
AAACTTGGAG GTCAGGCAGA GAAAAACAGG AAAATGCAGA AGAAGCACGA GTTGCTGGAG 
GAAGCCAGAA GACAGGGTTT GCCTTTTGCA CAGTGGGATG GGCCCACTGT GGTGGTGTGG 
CTGGAGCTGT GGGTGGGGAT GCCAGCCTGG TACGTGGCTG CCTGCCGAGC CAATGTGAAA 
AGCGGTGCTA TCATGTCAGC CCTGTCTGAC ACAGAAATCC AGCGGGAAAT CGGCATCAGC 
AATCCCCTGC ACAGGCTCAA GCTCCGCCTG GCCATCCAGG AAATCATGTC CCTAACCAGC 
CCCTCTGCCC CTCCCACCTC AAGGACGACC ACGGGAAATG TCTGGGTAAC ACATGAAGAG 
ATGGAAAATC TTACAGCCAC ACAACAAACG ACATTAGCCT ATGGTGACAT GAACCATGAG 
TGGATTGGCA ATGAGTGGCT GCCAAGCCTG GGGCTCCCTC AGTACCGCAG CTACTTCATG 
GAATGTCTGG TGGATGCTCG GATGCTGGAT CACTTAACTA AAAAAGACCT GCGTGGGCAG 
CTGAAAATGG TGGACAGCTT TCACAGAAAC AGTTTTCAGT GTGGAATTAT GTGCCTCCGA 
AGACTGAATT ACGATCGAAA AGAGCTAGAA AGAAAAAGAG AAGAAAGCCA GAATGAAATT 
AAGGATGTCC TTGTGTGGAG CAATGAGAGG ATGATCCACT GGGTCATGTC CATTGGCCTT 
AAGGAATATG CGAACAACCT GCTCGAGAGC GGAGTTCACG GTGCACTTGT GGCCTTAGAT 
GAATCCTTTG ATTACAATGC ATTAGCTCTC CTGCTGCAAA TCCCCACTCA AAACACACAG 
GCTCGTGCTG TTCTGGAGAG GGAATTCAAT AACCTTCTTG CTATGGGCAC TGACAGAAGA 
CTTGATGAAG ATGATGATAA GAGCTTTAGG AGAGCACCTT CGTGGAGAAA GAAGTTTAGA 
CCAAAGGACA TAAGAGGTTT AGCTACTGGA TCAGCAGAGA CTCTTCCTGC AAATTTCAGA 
GTTACAACCT CAATGTCTTC ACCCTCTATG CAGCCAAAGA AGATGCAGAT TGATGGCAGT 
GTATCAGGAA CACAAAGATT GGATTCTGCT ACAGTAAGGA CCTACTCCTG TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015486856.1
CDS	914..4687
Translation	MMCEVMPTISEAEIPAGGNGGHGSGSPLQSDADSHFEQLMVSMLEERDRLLETLRETQETLALTQGKLHEVGHERDSLQRQLNTALPQEFAALTKELNVCREQLLEREEEIAELKAERNNTRLLLEHLECLVSRHERSLRMTVVKRQAQSPAGVSSEVEVLKALKSLFEHHKALDEKVRERLRVALERCSLLEEELGTTHKELMILKEQNNQKKTLPDGMLDINHEQENTPTTNGKRSSDGSLCHDENLAKVIELQDIIDKQNKEQTQMKERLTALSSRVTELEEDLDTARKDLIKSEEMNTKLQRDVRETLAQKEDMEERITTLEKRYLAAQREATSVHDLNDKLENEIATKDSMHRQSEDKNRQLQERLELAEQKLQQTLRKAETLPEVEAELAQRVAALTKSELLSPGSSAAKDAKVLELATKLRKAEERHGNIEERLRQMEAQLEEKNQELQRARQREKMNEEHNKRLSETVDKLLSESNERLQLHLKERMAALEDKNSLLREIENAKKQIEELQHEKDQLVLNVDALRAENDQVRLRATSLHHSRPDFRFPVPPSSVGDTHTDSYSTSSVLRRPQKGRLAALRDEPSKVQTLNEQDWERAQQASVLANVAQAFESDVDVSDVEDDRETIFSSVDLLSPSGQADAQTLAIMLQEQLDAINKEIRLIQEEKENTEQRAEEIESRVGSGSLEAHARFRSLGSIPPAYGGALAGSSPPGSGRSTPRRIPHSPAREVDRLGIMTLPSDLRKHRRKSPASREEVRDDKTTIKCETSPPSSPRSLRLDRVQKGALHTVSHEDIRDIRNSTGSQDGQTSNPSSSNSSQDSLHKAPKKKGIKSSIGRLFGKKEKGRPGQGSKESLGQGGMGEADGSSQDALGLSKLGGQAEKNRKMQKKHELLEEARRQGLPFAQWDGPTVVVWLELWVGMPAWYVAACRANVKSGAIMSALSDTEIQREIGISNPLHRLKLRLAIQEIMSLTSPSAPPTSRTTTGNVWVTHEEMENLTATQQTTLAYGDMNHEWIGNEWLPSLGLPQYRSYFMECLVDARMLDHLTKKDLRGQLKMVDSFHRNSFQCGIMCLRRLNYDRKELERKREESQNEIKDVLVWSNERMIHWVMSIGLKEYANNLLESGVHGALVALDESFDYNALALLLQIPTQNTQARAVLEREFNNLLAMGTDRRLDEDDDKSFRRAPSWRKKFRPKDIRGLATGSAETLPANFRVTTSMSSPSMQPKKMQIDGSVSGTQRLDSATVRTYSC

Target ORF information:

RefSeq Version	XM_015631370.2
Organism	Parus major(Great Tit)
Definition	Parus major PTPRF interacting protein alpha 1 (PPFIA1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015631370.2

ORF Insert Sequence:

ATGATGTGTG AGGTGATGCC AACCATCAGT GAGGCCGAGA TTCCCGCCGG GGGAAATGGA 
GGCCACGGTT CAGGTTCCCC GTTGCAATCA GATGCTGATT CCCACTTCGA GCAGTTAATG 
GTGTCCATGC TGGAAGAAAG GGATCGCCTC CTAGAAACGC TGCGAGAAAC TCAAGAGACG 
CTTGCCCTCA CCCAGGGCAA GCTGCATGAG GTTGGCCATG AGAGGGATTC CTTGCAGAGG 
CAGCTCAATA CTGCACTTCC ACAGGAATTT GCAGCACTCA CCAAAGAGCT GAATGTATGC 
AGGGAGCAGC TGCTTGAAAG GGAAGAAGAA ATTGCTGAGC TGAAAGCAGA AAGAAATAAT 
ACGAGGCTAT TACTGGAACA TTTGGAGTGT CTTGTCTCCA GGCATGAGCG ATCCCTCAGG 
ATGACTGTTG TGAAGAGGCA AGCTCAGTCT CCAGCAGGAG TTTCTAGTGA AGTAGAAGTT 
CTCAAAGCAC TAAAATCTTT ATTTGAGCAC CACAAAGCCC TTGATGAAAA GGTAAGGGAG 
AGATTACGAG TAGCACTTGA AAGATGTAGC TTATTGGAAG AAGAACTAGG TACTACACAT 
AAAGAGTTAA TGATTCTGAA AGAGCAAAAT AATCAGAAGA AAACACTTCC AGATGGGATG 
CTGGATATAA ATCATGAACA AGAAAATACA CCAACTACAA ATGGGAAGAG ATCCTCTGAT 
GGTTCATTAT GCCATGATGA AAACCTTGCT AAAGTGATTG AACTCCAAGA CATCATAGAT 
AAGCAAAACA AAGAGCAGAC ACAAATGAAA GAACGCCTCA CTGCTTTGTC CAGCAGAGTA 
ACAGAGCTGG AAGAAGATCT AGACACAGCA AGGAAAGACT TAATAAAATC TGAAGAAATG 
AACACAAAGT TACAGAGGGA TGTACGAGAG ACTCTGGCCC AGAAGGAGGA CATGGAGGAG 
AGAATCACCA CTCTGGAGAA ACGCTACCTC GCTGCACAAC GTGAAGCTAC ATCCGTGCAT 
GACCTCAATG ATAAACTTGA AAATGAAATT GCCACTAAAG ACTCCATGCA CCGACAGAGT 
GAAGATAAGA ACAGGCAACT GCAAGAGCGA CTGGAACTGG CTGAGCAAAA GCTGCAGCAG 
ACCCTGAGAA AAGCTGAGAC TTTGCCAGAG GTGGAGGCTG AGCTGGCACA GAGAGTTGCA 
GCACTTACAA AGTCTGAACT TTTGTCTCCT GGGAGCTCTG CTGCTAAAGA TGCTAAAGTT 
TTGGAACTTG CCACCAAGCT TAGGAAGGCT GAGGAGAGAC ATGGCAATAT CGAGGAGCGA 
CTGAGACAGA TGGAGGCACA GCTTGAAGAG AAGAATCAAG AACTGCAAAG GGCTAGACAG 
AGAGAGAAGA TGAATGAAGA GCATAATAAA CGTTTGTCAG AAACTGTTGA TAAGCTCCTA 
TCTGAATCCA ATGAAAGGCT CCAACTTCAT CTCAAGGAGA GGATGGCTGC CCTGGAAGAT 
AAGAATTCAC TTCTTCGAGA AATTGAAAAT GCAAAGAAAC AAATAGAAGA ACTTCAGCAT 
GAAAAGGATC AACTTGTATT AAATGTGGAT GCATTAAGGG CTGAAAATGA CCAAGTGAGG 
CTCAGAGCCA CATCACTTCA TCATAGCAGA CCAGATTTCA GATTCCCTGT GCCACCCTCC 
TCTGTGGGGG ACACTCACAC AGACTCCTAC AGCACCTCCT CTGTGCTGAG GCGGCCCCAG 
AAGGGGCGTC TGGCTGCTCT CAGGGACGAG CCTTCAAAGG TTCAGACCCT GAATGAGCAG 
GATTGGGAGC GGGCCCAGCA GGCCAGTGTT TTGGCAAATG TGGCACAAGC ATTTGAGAGT 
GATGTGGATG TGTCTGATGT GGAGGATGAC AGGGAGACCA TTTTCAGCTC AGTTGATCTG 
CTGTCACCAA GTGGTCAGGC TGATGCTCAG ACTTTGGCCA TAATGCTGCA GGAACAGTTG 
GATGCAATCA ACAAAGAGAT CAGACTGATC CAGGAGGAGA AGGAGAACAC GGAGCAGCGC 
GCGGAGGAGA TTGAGAGCCG TGTGGGCAGC GGCAGCCTGG AGGCGCACGC CCGGTTCCGC 
TCGCTGGGCT CCATCCCCCC CGCCTACGGT GGGGCCCTGG CCGGCTCCTC CCCGCCCGGC 
AGCGGCCGCT CCACACCCCG GCGCATCCCA CACAGCCCGG CCAGGGAAGT GGACAGGCTG 
GGCATCATGA CATTGCCTAG TGATTTAAGG AAACACCGTA GAAAGTCTCC AGCTTCCAGG 
GAAGAGGTAC GAGATGACAA AACCACCATA AAATGTGAGA CATCACCACC TTCATCACCT 
CGGTCTTTGC GTCTGGACAG AGTCCAGAAA GGAGCTCTGC ACACAGTGAG CCACGAAGAT 
ATCAGGGACA TTAGGAATTC AACAGGTTCA CAAGATGGGC AAACAAGTAA TCCTAGTAGC 
AGTAACAGCA GCCAAGATTC CCTTCACAAA GCCCCCAAAA AAAAGGGAAT CAAATCCTCT 
ATTGGCCGCC TGTTTGGCAA GAAGGAAAAG GGACGACCTG GACAAGGAAG CAAGGAATCT 
CTAGGACAAG GTGGCATGGG AGAAGCAGAT GGTTCCTCTC AGGATGCATT AGGTCTCAGC 
AAACTTGGAG GTCAGGCAGA GAAAAACAGG AAAATGCAGA AGAAGCACGA GTTGCTGGAG 
GAAGCCAGAA GACAGGGTTT GCCTTTTGCA CAGTGGGATG GGCCCACTGT GGTGGTGTGG 
CTGGAGCTGT GGGTGGGGAT GCCAGCCTGG TACGTGGCTG CCTGCCGAGC CAATGTGAAA 
AGCGGTGCTA TCATGTCAGC CCTGTCTGAC ACAGAAATCC AGCGGGAAAT CGGCATCAGC 
AATCCCCTGC ACAGGCTCAA GCTCCGCCTG GCCATCCAGG AAATCATGTC CCTAACCAGC 
CCCTCTGCCC CTCCCACCTC AAGGACGACC ACGGGAAATG TCTGGGTAAC ACATGAAGAG 
ATGGAAAATC TTACAGCCAC ACAACAAACG ACATTAGCCT ATGGTGACAT GAACCATGAG 
TGGATTGGCA ATGAGTGGCT GCCAAGCCTG GGGCTCCCTC AGTACCGCAG CTACTTCATG 
GAATGTCTGG TGGATGCTCG GATGCTGGAT CACTTAACTA AAAAAGACCT GCGTGGGCAG 
CTGAAAATGG TGGACAGCTT TCACAGAAAC AGTTTTCAGT GTGGAATTAT GTGCCTCCGA 
AGACTGAATT ACGATCGAAA AGAGCTAGAA AGAAAAAGAG AAGAAAGCCA GAATGAAATT 
AAGGATGTCC TTGTGTGGAG CAATGAGAGG ATGATCCACT GGGTCATGTC CATTGGCCTT 
AAGGAATATG CGAACAACCT GCTCGAGAGC GGAGTTCACG GTGCACTTGT GGCCTTAGAT 
GAATCCTTTG ATTACAATGC ATTAGCTCTC CTGCTGCAAA TCCCCACTCA AAACACACAG 
GCTCGTGCTG TTCTGGAGAG GGAATTCAAT AACCTTCTTG CTATGGGCAC TGACAGAAGA 
CTTGATGAAG ATGATGATAA GAGCTTTAGG AGAGCACCTT CGTGGAGAAA GAAGTTTAGA 
CCAAAGGACA TAAGAGGTTT AGCTACTGGA TCAGCAGAGA CTCTTCCTGC AAATTTCAGA 
GTTACAACCT CAATGTCTTC ACCCTCTATG CAGCCAAAGA AGATGCAGAT TGATGGCAGT 
GTATCAGGAA CACAAAGATT GGATTCTGCT ACAGTAAGGA CCTACTCCTG TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PPFIA1 ( XM_015631371.2 ) cDNA ORF clone, Parus major(Great Tit) -> XP_015486857.1 Parus major PTPRF interacting protein alpha 1 (PPFIA1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OPa179671
Clone ID Related Accession (Same CDS sequence)	XM_015631371.2
Accession Version	XM_015631371.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3771bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-11-13
Organism	Parus major(Great Tit)
Product	liprin-alpha-1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_031774.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Nov 4, 2016 this sequence version replaced XM_015631371.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Parus major Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTGTG AGGTGATGCC AACCATCAGT GAGGCCGAGA TTCCCGCCGG GGGAAATGGA 
GGCCACGGTT CAGGTTCCCC GTTGCAATCA GATGCTGATT CCCACTTCGA GCAGTTAATG 
GTGTCCATGC TGGAAGAAAG GGATCGCCTC CTAGAAACGC TGCGAGAAAC TCAAGAGACG 
CTTGCCCTCA CCCAGGGCAA GCTGCATGAG GTTGGCCATG AGAGGGATTC CTTGCAGAGG 
CAGCTCAATA CTGCACTTCC ACAGGAATTT GCAGCACTCA CCAAAGAGCT GAATGTATGC 
AGGGAGCAGC TGCTTGAAAG GGAAGAAGAA ATTGCTGAGC TGAAAGCAGA AAGAAATAAT 
ACGAGGCTAT TACTGGAACA TTTGGAGTGT CTTGTCTCCA GGCATGAGCG ATCCCTCAGG 
ATGACTGTTG TGAAGAGGCA AGCTCAGTCT CCAGCAGGAG TTTCTAGTGA AGTAGAAGTT 
CTCAAAGCAC TAAAATCTTT ATTTGAGCAC CACAAAGCCC TTGATGAAAA GGTAAGGGAG 
AGATTACGAG TAGCACTTGA AAGATGTAGC TTATTGGAAG AAGAACTAGG TACTACACAT 
AAAGAGTTAA TGATTCTGAA AGAGCAAAAT AATCAGAAGA AAACACTTCC AGATGGGATG 
CTGGATATAA ATCATGAACA AGAAAATACA CCAACTACAA ATGGGAAGAG ATCCTCTGAT 
GGTTCATTAT GCCATGATGA AAACCTTGCT AAAGTGATTG AACTCCAAGA CATCATAGAT 
AAGCAAAACA AAGAGCAGAC ACAAATGAAA GAACGCCTCA CTGCTTTGTC CAGCAGAGTA 
ACAGAGCTGG AAGAAGATCT AGACACAGCA AGGAAAGACT TAATAAAATC TGAAGAAATG 
AACACAAAGT TACAGAGGGA TGTACGAGAG ACTCTGGCCC AGAAGGAGGA CATGGAGGAG 
AGAATCACCA CTCTGGAGAA ACGCTACCTC GCTGCACAAC GTGAAGCTAC ATCCGTGCAT 
GACCTCAATG ATAAACTTGA AAATGAAATT GCCACTAAAG ACTCCATGCA CCGACAGAGT 
GAAGATAAGA ACAGGCAACT GCAAGAGCGA CTGGAACTGG CTGAGCAAAA GCTGCAGCAG 
ACCCTGAGAA AAGCTGAGAC TTTGCCAGAG GTGGAGGCTG AGCTGGCACA GAGAGTTGCA 
GCACTTACAA AGTCTGAACT TTTGTCTCCT GGGAGCTCTG CTGCTAAAGA TGCTAAAGTT 
TTGGAACTTG CCACCAAGCT TAGGAAGGCT GAGGAGAGAC ATGGCAATAT CGAGGAGCGA 
CTGAGACAGA TGGAGGCACA GCTTGAAGAG AAGAATCAAG AACTGCAAAG GGCTAGACAG 
AGAGAGAAGA TGAATGAAGA GCATAATAAA CGTTTGTCAG AAACTGTTGA TAAGCTCCTA 
TCTGAATCCA ATGAAAGGCT CCAACTTCAT CTCAAGGAGA GGATGGCTGC CCTGGAAGAT 
AAGAATTCAC TTCTTCGAGA AATTGAAAAT GCAAAGAAAC AAATAGAAGA ACTTCAGCAT 
GAAAAGGATC AACTTGTATT AAATGTGGAT GCATTAAGGG CTGAAAATGA CCAAGTGAGG 
CTCAGAGCCA CATCACTTCA TCATAGCAGA CCAGATTTCA GATTCCCTGT GCCACCCTCC 
TCTGTGGGGG ACACTCACAC AGACTCCTAC AGCACCTCCT CTGTGCTGAG GCGGCCCCAG 
AAGGGGCGTC TGGCTGCTCT CAGGGACGAG CCTTCAAAGG TTCAGACCCT GAATGAGCAG 
GATTGGGAGC GGGCCCAGCA GGCCAGTGTT TTGGCAAATG TGGCACAAGC ATTTGAGAGT 
GATGTGGATG TGTCTGATGT GGAGGATGAC AGGGAGACCA TTTTCAGCTC AGTTGATCTG 
CTGTCACCAA GTGGTCAGGC TGATGCTCAG ACTTTGGCCA TAATGCTGCA GGAACAGTTG 
GATGCAATCA ACAAAGAGAT CAGACTGATC CAGGAGGAGA AGGAGAACAC GGAGCAGCGC 
GCGGAGGAGA TTGAGAGCCG TGTGGGCAGC GGCAGCCTGG AGGCGCACGC CCGGTTCCGC 
TCGCTGGGCT CCATCCCCCC CGCCTACGGT GGGGCCCTGG CCGGCTCCTC CCCGCCCGGC 
AGCGGCCGCT CCACACCCCG GCGCATCCCA CACAGCCCGG CCAGGGAAGT GGACAGGCTG 
GGCATCATGA CATTGTCTCC AGCTTCCAGG GAAGAGGTAC GAGATGACAA AACCACCATA 
AAATGTGAGA CATCACCACC TTCATCACCT CGGTCTTTGC GTCTGGACAG AGTCCAGAAA 
GGAGCTCTGC ACACAGTGAG CCACGAAGAT ATCAGGGACA TTAGGAATTC AACAGGTTCA 
CAAGATGGGC AAACAAGTAA TCCTAGTAGC AGTAACAGCA GCCAAGATTC CCTTCACAAA 
GCCCCCAAAA AAAAGGGAAT CAAATCCTCT ATTGGCCGCC TGTTTGGCAA GAAGGAAAAG 
GGACGACCTG GACAAGGAAG CAAGGAATCT CTAGGACAAG GTGGCATGGG AGAAGCAGAT 
GGTTCCTCTC AGGATGCATT AGGTCTCAGC AAACTTGGAG GTCAGGCAGA GAAAAACAGG 
AAAATGCAGA AGAAGCACGA GTTGCTGGAG GAAGCCAGAA GACAGGGTTT GCCTTTTGCA 
CAGTGGGATG GGCCCACTGT GGTGGTGTGG CTGGAGCTGT GGGTGGGGAT GCCAGCCTGG 
TACGTGGCTG CCTGCCGAGC CAATGTGAAA AGCGGTGCTA TCATGTCAGC CCTGTCTGAC 
ACAGAAATCC AGCGGGAAAT CGGCATCAGC AATCCCCTGC ACAGGCTCAA GCTCCGCCTG 
GCCATCCAGG AAATCATGTC CCTAACCAGC CCCTCTGCCC CTCCCACCTC AAGGACGACC 
ACGGGAAATG TCTGGGTAAC ACATGAAGAG ATGGAAAATC TTACAGCCAC ACAACAAACG 
GAAGATGAGG AGGGAAGCTG GGCTCAGACA TTAGCCTATG GTGACATGAA CCATGAGTGG 
ATTGGCAATG AGTGGCTGCC AAGCCTGGGG CTCCCTCAGT ACCGCAGCTA CTTCATGGAA 
TGTCTGGTGG ATGCTCGGAT GCTGGATCAC TTAACTAAAA AAGACCTGCG TGGGCAGCTG 
AAAATGGTGG ACAGCTTTCA CAGAAACAGT TTTCAGTGTG GAATTATGTG CCTCCGAAGA 
CTGAATTACG ATCGAAAAGA GCTAGAAAGA AAAAGAGAAG AAAGCCAGAA TGAAATTAAG 
GATGTCCTTG TGTGGAGCAA TGAGAGGATG ATCCACTGGG TCATGTCCAT TGGCCTTAAG 
GAATATGCGA ACAACCTGCT CGAGAGCGGA GTTCACGGTG CACTTGTGGC CTTAGATGAA 
TCCTTTGATT ACAATGCATT AGCTCTCCTG CTGCAAATCC CCACTCAAAA CACACAGGCT 
CGTGCTGTTC TGGAGAGGGA ATTCAATAAC CTTCTTGCTA TGGGCACTGA CAGAAGACTT 
GATGAAGATG ATGATAAGAG CTTTAGGAGA GCACCTTCGT GGAGAAAGAA GTTTAGACCA 
AAGGACATAA GAGGTTTAGC TACTGGATCA GCAGAGACTC TTCCTGCAAA TTTCAGAGTT 
ACAACCTCAA TGTCTTCACC CTCTATGCAG CCAAAGAAGA TGCAGATTGA TGGCAGTGTA 
TCAGGAACAC AAAGATTGGA TTCTGCTACA GTAAGGACCT ACTCCTGTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015486857.1
CDS	914..4684
Translation	MMCEVMPTISEAEIPAGGNGGHGSGSPLQSDADSHFEQLMVSMLEERDRLLETLRETQETLALTQGKLHEVGHERDSLQRQLNTALPQEFAALTKELNVCREQLLEREEEIAELKAERNNTRLLLEHLECLVSRHERSLRMTVVKRQAQSPAGVSSEVEVLKALKSLFEHHKALDEKVRERLRVALERCSLLEEELGTTHKELMILKEQNNQKKTLPDGMLDINHEQENTPTTNGKRSSDGSLCHDENLAKVIELQDIIDKQNKEQTQMKERLTALSSRVTELEEDLDTARKDLIKSEEMNTKLQRDVRETLAQKEDMEERITTLEKRYLAAQREATSVHDLNDKLENEIATKDSMHRQSEDKNRQLQERLELAEQKLQQTLRKAETLPEVEAELAQRVAALTKSELLSPGSSAAKDAKVLELATKLRKAEERHGNIEERLRQMEAQLEEKNQELQRARQREKMNEEHNKRLSETVDKLLSESNERLQLHLKERMAALEDKNSLLREIENAKKQIEELQHEKDQLVLNVDALRAENDQVRLRATSLHHSRPDFRFPVPPSSVGDTHTDSYSTSSVLRRPQKGRLAALRDEPSKVQTLNEQDWERAQQASVLANVAQAFESDVDVSDVEDDRETIFSSVDLLSPSGQADAQTLAIMLQEQLDAINKEIRLIQEEKENTEQRAEEIESRVGSGSLEAHARFRSLGSIPPAYGGALAGSSPPGSGRSTPRRIPHSPAREVDRLGIMTLSPASREEVRDDKTTIKCETSPPSSPRSLRLDRVQKGALHTVSHEDIRDIRNSTGSQDGQTSNPSSSNSSQDSLHKAPKKKGIKSSIGRLFGKKEKGRPGQGSKESLGQGGMGEADGSSQDALGLSKLGGQAEKNRKMQKKHELLEEARRQGLPFAQWDGPTVVVWLELWVGMPAWYVAACRANVKSGAIMSALSDTEIQREIGISNPLHRLKLRLAIQEIMSLTSPSAPPTSRTTTGNVWVTHEEMENLTATQQTEDEEGSWAQTLAYGDMNHEWIGNEWLPSLGLPQYRSYFMECLVDARMLDHLTKKDLRGQLKMVDSFHRNSFQCGIMCLRRLNYDRKELERKREESQNEIKDVLVWSNERMIHWVMSIGLKEYANNLLESGVHGALVALDESFDYNALALLLQIPTQNTQARAVLEREFNNLLAMGTDRRLDEDDDKSFRRAPSWRKKFRPKDIRGLATGSAETLPANFRVTTSMSSPSMQPKKMQIDGSVSGTQRLDSATVRTYSC

Target ORF information:

RefSeq Version	XM_015631371.2
Organism	Parus major(Great Tit)
Definition	Parus major PTPRF interacting protein alpha 1 (PPFIA1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015631371.2

ORF Insert Sequence:

ATGATGTGTG AGGTGATGCC AACCATCAGT GAGGCCGAGA TTCCCGCCGG GGGAAATGGA 
GGCCACGGTT CAGGTTCCCC GTTGCAATCA GATGCTGATT CCCACTTCGA GCAGTTAATG 
GTGTCCATGC TGGAAGAAAG GGATCGCCTC CTAGAAACGC TGCGAGAAAC TCAAGAGACG 
CTTGCCCTCA CCCAGGGCAA GCTGCATGAG GTTGGCCATG AGAGGGATTC CTTGCAGAGG 
CAGCTCAATA CTGCACTTCC ACAGGAATTT GCAGCACTCA CCAAAGAGCT GAATGTATGC 
AGGGAGCAGC TGCTTGAAAG GGAAGAAGAA ATTGCTGAGC TGAAAGCAGA AAGAAATAAT 
ACGAGGCTAT TACTGGAACA TTTGGAGTGT CTTGTCTCCA GGCATGAGCG ATCCCTCAGG 
ATGACTGTTG TGAAGAGGCA AGCTCAGTCT CCAGCAGGAG TTTCTAGTGA AGTAGAAGTT 
CTCAAAGCAC TAAAATCTTT ATTTGAGCAC CACAAAGCCC TTGATGAAAA GGTAAGGGAG 
AGATTACGAG TAGCACTTGA AAGATGTAGC TTATTGGAAG AAGAACTAGG TACTACACAT 
AAAGAGTTAA TGATTCTGAA AGAGCAAAAT AATCAGAAGA AAACACTTCC AGATGGGATG 
CTGGATATAA ATCATGAACA AGAAAATACA CCAACTACAA ATGGGAAGAG ATCCTCTGAT 
GGTTCATTAT GCCATGATGA AAACCTTGCT AAAGTGATTG AACTCCAAGA CATCATAGAT 
AAGCAAAACA AAGAGCAGAC ACAAATGAAA GAACGCCTCA CTGCTTTGTC CAGCAGAGTA 
ACAGAGCTGG AAGAAGATCT AGACACAGCA AGGAAAGACT TAATAAAATC TGAAGAAATG 
AACACAAAGT TACAGAGGGA TGTACGAGAG ACTCTGGCCC AGAAGGAGGA CATGGAGGAG 
AGAATCACCA CTCTGGAGAA ACGCTACCTC GCTGCACAAC GTGAAGCTAC ATCCGTGCAT 
GACCTCAATG ATAAACTTGA AAATGAAATT GCCACTAAAG ACTCCATGCA CCGACAGAGT 
GAAGATAAGA ACAGGCAACT GCAAGAGCGA CTGGAACTGG CTGAGCAAAA GCTGCAGCAG 
ACCCTGAGAA AAGCTGAGAC TTTGCCAGAG GTGGAGGCTG AGCTGGCACA GAGAGTTGCA 
GCACTTACAA AGTCTGAACT TTTGTCTCCT GGGAGCTCTG CTGCTAAAGA TGCTAAAGTT 
TTGGAACTTG CCACCAAGCT TAGGAAGGCT GAGGAGAGAC ATGGCAATAT CGAGGAGCGA 
CTGAGACAGA TGGAGGCACA GCTTGAAGAG AAGAATCAAG AACTGCAAAG GGCTAGACAG 
AGAGAGAAGA TGAATGAAGA GCATAATAAA CGTTTGTCAG AAACTGTTGA TAAGCTCCTA 
TCTGAATCCA ATGAAAGGCT CCAACTTCAT CTCAAGGAGA GGATGGCTGC CCTGGAAGAT 
AAGAATTCAC TTCTTCGAGA AATTGAAAAT GCAAAGAAAC AAATAGAAGA ACTTCAGCAT 
GAAAAGGATC AACTTGTATT AAATGTGGAT GCATTAAGGG CTGAAAATGA CCAAGTGAGG 
CTCAGAGCCA CATCACTTCA TCATAGCAGA CCAGATTTCA GATTCCCTGT GCCACCCTCC 
TCTGTGGGGG ACACTCACAC AGACTCCTAC AGCACCTCCT CTGTGCTGAG GCGGCCCCAG 
AAGGGGCGTC TGGCTGCTCT CAGGGACGAG CCTTCAAAGG TTCAGACCCT GAATGAGCAG 
GATTGGGAGC GGGCCCAGCA GGCCAGTGTT TTGGCAAATG TGGCACAAGC ATTTGAGAGT 
GATGTGGATG TGTCTGATGT GGAGGATGAC AGGGAGACCA TTTTCAGCTC AGTTGATCTG 
CTGTCACCAA GTGGTCAGGC TGATGCTCAG ACTTTGGCCA TAATGCTGCA GGAACAGTTG 
GATGCAATCA ACAAAGAGAT CAGACTGATC CAGGAGGAGA AGGAGAACAC GGAGCAGCGC 
GCGGAGGAGA TTGAGAGCCG TGTGGGCAGC GGCAGCCTGG AGGCGCACGC CCGGTTCCGC 
TCGCTGGGCT CCATCCCCCC CGCCTACGGT GGGGCCCTGG CCGGCTCCTC CCCGCCCGGC 
AGCGGCCGCT CCACACCCCG GCGCATCCCA CACAGCCCGG CCAGGGAAGT GGACAGGCTG 
GGCATCATGA CATTGTCTCC AGCTTCCAGG GAAGAGGTAC GAGATGACAA AACCACCATA 
AAATGTGAGA CATCACCACC TTCATCACCT CGGTCTTTGC GTCTGGACAG AGTCCAGAAA 
GGAGCTCTGC ACACAGTGAG CCACGAAGAT ATCAGGGACA TTAGGAATTC AACAGGTTCA 
CAAGATGGGC AAACAAGTAA TCCTAGTAGC AGTAACAGCA GCCAAGATTC CCTTCACAAA 
GCCCCCAAAA AAAAGGGAAT CAAATCCTCT ATTGGCCGCC TGTTTGGCAA GAAGGAAAAG 
GGACGACCTG GACAAGGAAG CAAGGAATCT CTAGGACAAG GTGGCATGGG AGAAGCAGAT 
GGTTCCTCTC AGGATGCATT AGGTCTCAGC AAACTTGGAG GTCAGGCAGA GAAAAACAGG 
AAAATGCAGA AGAAGCACGA GTTGCTGGAG GAAGCCAGAA GACAGGGTTT GCCTTTTGCA 
CAGTGGGATG GGCCCACTGT GGTGGTGTGG CTGGAGCTGT GGGTGGGGAT GCCAGCCTGG 
TACGTGGCTG CCTGCCGAGC CAATGTGAAA AGCGGTGCTA TCATGTCAGC CCTGTCTGAC 
ACAGAAATCC AGCGGGAAAT CGGCATCAGC AATCCCCTGC ACAGGCTCAA GCTCCGCCTG 
GCCATCCAGG AAATCATGTC CCTAACCAGC CCCTCTGCCC CTCCCACCTC AAGGACGACC 
ACGGGAAATG TCTGGGTAAC ACATGAAGAG ATGGAAAATC TTACAGCCAC ACAACAAACG 
GAAGATGAGG AGGGAAGCTG GGCTCAGACA TTAGCCTATG GTGACATGAA CCATGAGTGG 
ATTGGCAATG AGTGGCTGCC AAGCCTGGGG CTCCCTCAGT ACCGCAGCTA CTTCATGGAA 
TGTCTGGTGG ATGCTCGGAT GCTGGATCAC TTAACTAAAA AAGACCTGCG TGGGCAGCTG 
AAAATGGTGG ACAGCTTTCA CAGAAACAGT TTTCAGTGTG GAATTATGTG CCTCCGAAGA 
CTGAATTACG ATCGAAAAGA GCTAGAAAGA AAAAGAGAAG AAAGCCAGAA TGAAATTAAG 
GATGTCCTTG TGTGGAGCAA TGAGAGGATG ATCCACTGGG TCATGTCCAT TGGCCTTAAG 
GAATATGCGA ACAACCTGCT CGAGAGCGGA GTTCACGGTG CACTTGTGGC CTTAGATGAA 
TCCTTTGATT ACAATGCATT AGCTCTCCTG CTGCAAATCC CCACTCAAAA CACACAGGCT 
CGTGCTGTTC TGGAGAGGGA ATTCAATAAC CTTCTTGCTA TGGGCACTGA CAGAAGACTT 
GATGAAGATG ATGATAAGAG CTTTAGGAGA GCACCTTCGT GGAGAAAGAA GTTTAGACCA 
AAGGACATAA GAGGTTTAGC TACTGGATCA GCAGAGACTC TTCCTGCAAA TTTCAGAGTT 
ACAACCTCAA TGTCTTCACC CTCTATGCAG CCAAAGAAGA TGCAGATTGA TGGCAGTGTA 
TCAGGAACAC AAAGATTGGA TTCTGCTACA GTAAGGACCT ACTCCTGTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PPFIA1 ( XM_015631372.2 ) cDNA ORF clone, Parus major(Great Tit) -> XP_015486858.1 Parus major PTPRF interacting protein alpha 1 (PPFIA1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OPa179672
Clone ID Related Accession (Same CDS sequence)	XM_015631372.2
Accession Version	XM_015631372.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3768bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-11-13
Organism	Parus major(Great Tit)
Product	liprin-alpha-1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_031774.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Nov 4, 2016 this sequence version replaced XM_015631372.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Parus major Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTGTG AGGTGATGCC AACCATCAGT GAGGCCGAGA TTCCCGCCGG GGGAAATGGA 
GGCCACGGTT CAGGTTCCCC GTTGCAATCA GATGCTGATT CCCACTTCGA GCAGTTAATG 
GTGTCCATGC TGGAAGAAAG GGATCGCCTC CTAGAAACGC TGCGAGAAAC TCAAGAGACG 
CTTGCCCTCA CCCAGGGCAA GCTGCATGAG GTTGGCCATG AGAGGGATTC CTTGCAGAGG 
CAGCTCAATA CTGCACTTCC ACAGGAATTT GCAGCACTCA CCAAAGAGCT GAATGTATGC 
AGGGAGCAGC TGCTTGAAAG GGAAGAAGAA ATTGCTGAGC TGAAAGCAGA AAGAAATAAT 
ACGAGGCTAT TACTGGAACA TTTGGAGTGT CTTGTCTCCA GGCATGAGCG ATCCCTCAGG 
ATGACTGTTG TGAAGAGGCA AGCTCAGTCT CCAGCAGGAG TTTCTAGTGA AGTAGAAGTT 
CTCAAAGCAC TAAAATCTTT ATTTGAGCAC CACAAAGCCC TTGATGAAAA GGTAAGGGAG 
AGATTACGAG TAGCACTTGA AAGATGTAGC TTATTGGAAG AAGAACTAGG TACTACACAT 
AAAGAGTTAA TGATTCTGAA AGAGCAAAAT AATCAGAAGA AAACACTTCC AGATGGGATG 
CTGGATATAA ATCATGAACA AGAAAATACA CCAACTACAA ATGGGAAGAG ATCCTCTGAT 
GGTTCATTAT GCCATGATGA AAACCTTGCT AAAGTGATTG AACTCCAAGA CATCATAGAT 
AAGCAAAACA AAGAGCAGAC ACAAATGAAA GAACGCCTCA CTGCTTTGTC CAGCAGAGTA 
ACAGAGCTGG AAGAAGATCT AGACACAGCA AGGAAAGACT TAATAAAATC TGAAGAAATG 
AACACAAAGT TACAGAGGGA TGTACGAGAG ACTCTGGCCC AGAAGGAGGA CATGGAGGAG 
AGAATCACCA CTCTGGAGAA ACGCTACCTC GCTGCACAAC GTGAAGCTAC ATCCGTGCAT 
GACCTCAATG ATAAACTTGA AAATGAAATT GCCACTAAAG ACTCCATGCA CCGACAGAGT 
GAAGATAAGA ACAGGCAACT GCAAGAGCGA CTGGAACTGG CTGAGCAAAA GCTGCAGCAG 
ACCCTGAGAA AAGCTGAGAC TTTGCCAGAG GTGGAGGCTG AGCTGGCACA GAGAGTTGCA 
GCACTTACAA AGTCTGAACT TTTGTCTCCT GGGAGCTCTG CTGCTAAAGA TGCTAAAGTT 
TTGGAACTTG CCACCAAGCT TAGGAAGGCT GAGGAGAGAC ATGGCAATAT CGAGGAGCGA 
CTGAGACAGA TGGAGGCACA GCTTGAAGAG AAGAATCAAG AACTGCAAAG GGCTAGACAG 
AGAGAGAAGA TGAATGAAGA GCATAATAAA CGTTTGTCAG AAACTGTTGA TAAGCTCCTA 
TCTGAATCCA ATGAAAGGCT CCAACTTCAT CTCAAGGAGA GGATGGCTGC CCTGGAAGAT 
AAGAATTCAC TTCTTCGAGA AATTGAAAAT GCAAAGAAAC AAATAGAAGA ACTTCAGCAT 
GAAAAGGATC AACTTGTATT AAATGTGGAT GCATTAAGGG CTGAAAATGA CCAAGTGAGG 
CTCAGAGCCA CATCACTTCA TCATAGCAGA CCAGATTTCA GATTCCCTGT GCCACCCTCC 
TCTGTGGGGG ACACTCACAC AGACTCCTAC AGCACCTCCT CTGTGCTGAG GCGGCCCCAG 
AAGGGGCGTC TGGCTGCTCT CAGGGACGAG CCTTCAAAGG TTCAGACCCT GAATGAGCAG 
GATTGGGAGC GGGCCCAGCA GGCCAGTGTT TTGGCAAATG TGGCACAAGC ATTTGAGAGT 
GATGTGGATG TGTCTGATGT GGAGGATGAC AGGGAGACCA TTTTCAGCTC AGTTGATCTG 
CTGTCACCAA GTGGTCAGGC TGATGCTCAG ACTTTGGCCA TAATGCTGCA GGAACAGTTG 
GATGCAATCA ACAAAGAGAT CAGACTGATC CAGGAGGAGA AGGAGAACAC GGAGCAGCGC 
GCGGAGGAGA TTGAGAGCCG TGTGGGCAGC GGCAGCCTGG AGGCGCACGC CCGGTTCCGC 
TCGCTGGGCT CCATCCCCCC CGCCTACGGT GGGGCCCTGG CCGGCTCCTC CCCGCCCGGC 
AGCGGCCGCT CCACACCCCG GCGCATCCCA CACAGCCCGG CCAGGGAAGT GGACAGGCTG 
GGCATCATGA CATTGCCTAG TGATTTAAGG AAACACCGTA GAAAGTCTCC AGCTTCCAGG 
GAAGAGGTAC GAGATGACAA AACCACCATA AAATGTGAGA CATCACCACC TTCATCACCT 
CGGTCTTTGC GTCTGGACAG AGTCCAGAAA GGAGCTCTGC ACACAGTGAG CCACGAAGAT 
ATCAGGGACA TTAGGAATTC AACAGGTTCA CAAGATGGGC AAACAAGTAA TCCTAGTAGC 
AGTAACAGCA GCCAAGATTC CCTTCACAAA GCCCCCAAAA AAAAGGGAAT CAAATCCTCT 
ATTGGCCGCC TGTTTGGCAA GAAGGAAAAG GGACGACCTG GACAAGGAAG CAAGGAATCT 
CTAGGACAAG GTGGCATGGG AGAAGCAGAT GGTTCCTCTC AGGATGCATT AGGTCTCAGC 
AAACTTGGAG GTCAGGCAGA GAAAAACAGG AAAATGCAGA AGAAGCACGA GTTGCTGGAG 
GAAGCCAGAA GACAGGGTTT GCCTTTTGCA CAGTGGGATG GGCCCACTGT GGTGGTGTGG 
CTGGAGCTGT GGGTGGGGAT GCCAGCCTGG TACGTGGCTG CCTGCCGAGC CAATGTGAAA 
AGCGGTGCTA TCATGTCAGC CCTGTCTGAC ACAGAAATCC AGCGGGAAAT CGGCATCAGC 
AATCCCCTGC ACAGGCTCAA GCTCCGCCTG GCCATCCAGG AAATCATGTC CCTAACCAGC 
CCCTCTGCCC CTCCCACCTC AAGGACGACC ACGGGAAATG TCTGGGTAAC ACATGAAGAG 
ATGGAAAATC TTACAGCCAC ACAACAAACG GAAGATGAGG AGGGAAGCTG GGCTCAGACA 
TTAGCCTATG GTGACATGAA CCATGAGTGG ATTGGCAATG AGTGGCTGCC AAGCCTGGGG 
CTCCCTCAGT ACCGCAGCTA CTTCATGGAA TGTCTGGTGG ATGCTCGGAT GCTGGATCAC 
TTAACTAAAA AAGACCTGCG TGGGCAGCTG AAAATGGTGG ACAGCTTTCA CAGAAACAGT 
TTTCAGTGTG GAATTATGTG CCTCCGAAGA CTGAATTACG ATCGAAAAGA GCTAGAAAGA 
AAAAGAGAAG AAAGCCAGAA TGAAATTAAG GATGTCCTTG TGTGGAGCAA TGAGAGGATG 
ATCCACTGGG TCATGTCCAT TGGCCTTAAG GAATATGCGA ACAACCTGCT CGAGAGCGGA 
GTTCACGGTG CACTTGTGGC CTTAGATGAA TCCTTTGATT ACAATGCATT AGCTCTCCTG 
CTGCAAATCC CCACTCAAAA CACACAGGCT CGTGCTGTTC TGGAGAGGGA ATTCAATAAC 
CTTCTTGCTA TGGGCACTGA CAGAAGACTT GATGAAGATG ATGATAAGAG CTTTAGGAGA 
GCACCTTCGT GGAGAAAGAA GTTTAGACCA AAGGACATAA GAGGTTTAGC TACTGGATCA 
GCAGAGACTC TTCCTGCAAA TTTCAGAGTT ACAACCTCAA TGTCTTCACC CTCTATGCAG 
CCAAAGAAGA TGCAGATTGA TGTTTACCCA CACTATTTCT ACCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015486858.1
CDS	915..4682
Translation	MMCEVMPTISEAEIPAGGNGGHGSGSPLQSDADSHFEQLMVSMLEERDRLLETLRETQETLALTQGKLHEVGHERDSLQRQLNTALPQEFAALTKELNVCREQLLEREEEIAELKAERNNTRLLLEHLECLVSRHERSLRMTVVKRQAQSPAGVSSEVEVLKALKSLFEHHKALDEKVRERLRVALERCSLLEEELGTTHKELMILKEQNNQKKTLPDGMLDINHEQENTPTTNGKRSSDGSLCHDENLAKVIELQDIIDKQNKEQTQMKERLTALSSRVTELEEDLDTARKDLIKSEEMNTKLQRDVRETLAQKEDMEERITTLEKRYLAAQREATSVHDLNDKLENEIATKDSMHRQSEDKNRQLQERLELAEQKLQQTLRKAETLPEVEAELAQRVAALTKSELLSPGSSAAKDAKVLELATKLRKAEERHGNIEERLRQMEAQLEEKNQELQRARQREKMNEEHNKRLSETVDKLLSESNERLQLHLKERMAALEDKNSLLREIENAKKQIEELQHEKDQLVLNVDALRAENDQVRLRATSLHHSRPDFRFPVPPSSVGDTHTDSYSTSSVLRRPQKGRLAALRDEPSKVQTLNEQDWERAQQASVLANVAQAFESDVDVSDVEDDRETIFSSVDLLSPSGQADAQTLAIMLQEQLDAINKEIRLIQEEKENTEQRAEEIESRVGSGSLEAHARFRSLGSIPPAYGGALAGSSPPGSGRSTPRRIPHSPAREVDRLGIMTLPSDLRKHRRKSPASREEVRDDKTTIKCETSPPSSPRSLRLDRVQKGALHTVSHEDIRDIRNSTGSQDGQTSNPSSSNSSQDSLHKAPKKKGIKSSIGRLFGKKEKGRPGQGSKESLGQGGMGEADGSSQDALGLSKLGGQAEKNRKMQKKHELLEEARRQGLPFAQWDGPTVVVWLELWVGMPAWYVAACRANVKSGAIMSALSDTEIQREIGISNPLHRLKLRLAIQEIMSLTSPSAPPTSRTTTGNVWVTHEEMENLTATQQTEDEEGSWAQTLAYGDMNHEWIGNEWLPSLGLPQYRSYFMECLVDARMLDHLTKKDLRGQLKMVDSFHRNSFQCGIMCLRRLNYDRKELERKREESQNEIKDVLVWSNERMIHWVMSIGLKEYANNLLESGVHGALVALDESFDYNALALLLQIPTQNTQARAVLEREFNNLLAMGTDRRLDEDDDKSFRRAPSWRKKFRPKDIRGLATGSAETLPANFRVTTSMSSPSMQPKKMQIDVYPHYFYR

Target ORF information:

RefSeq Version	XM_015631372.2
Organism	Parus major(Great Tit)
Definition	Parus major PTPRF interacting protein alpha 1 (PPFIA1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015631372.2

ORF Insert Sequence:

ATGATGTGTG AGGTGATGCC AACCATCAGT GAGGCCGAGA TTCCCGCCGG GGGAAATGGA 
GGCCACGGTT CAGGTTCCCC GTTGCAATCA GATGCTGATT CCCACTTCGA GCAGTTAATG 
GTGTCCATGC TGGAAGAAAG GGATCGCCTC CTAGAAACGC TGCGAGAAAC TCAAGAGACG 
CTTGCCCTCA CCCAGGGCAA GCTGCATGAG GTTGGCCATG AGAGGGATTC CTTGCAGAGG 
CAGCTCAATA CTGCACTTCC ACAGGAATTT GCAGCACTCA CCAAAGAGCT GAATGTATGC 
AGGGAGCAGC TGCTTGAAAG GGAAGAAGAA ATTGCTGAGC TGAAAGCAGA AAGAAATAAT 
ACGAGGCTAT TACTGGAACA TTTGGAGTGT CTTGTCTCCA GGCATGAGCG ATCCCTCAGG 
ATGACTGTTG TGAAGAGGCA AGCTCAGTCT CCAGCAGGAG TTTCTAGTGA AGTAGAAGTT 
CTCAAAGCAC TAAAATCTTT ATTTGAGCAC CACAAAGCCC TTGATGAAAA GGTAAGGGAG 
AGATTACGAG TAGCACTTGA AAGATGTAGC TTATTGGAAG AAGAACTAGG TACTACACAT 
AAAGAGTTAA TGATTCTGAA AGAGCAAAAT AATCAGAAGA AAACACTTCC AGATGGGATG 
CTGGATATAA ATCATGAACA AGAAAATACA CCAACTACAA ATGGGAAGAG ATCCTCTGAT 
GGTTCATTAT GCCATGATGA AAACCTTGCT AAAGTGATTG AACTCCAAGA CATCATAGAT 
AAGCAAAACA AAGAGCAGAC ACAAATGAAA GAACGCCTCA CTGCTTTGTC CAGCAGAGTA 
ACAGAGCTGG AAGAAGATCT AGACACAGCA AGGAAAGACT TAATAAAATC TGAAGAAATG 
AACACAAAGT TACAGAGGGA TGTACGAGAG ACTCTGGCCC AGAAGGAGGA CATGGAGGAG 
AGAATCACCA CTCTGGAGAA ACGCTACCTC GCTGCACAAC GTGAAGCTAC ATCCGTGCAT 
GACCTCAATG ATAAACTTGA AAATGAAATT GCCACTAAAG ACTCCATGCA CCGACAGAGT 
GAAGATAAGA ACAGGCAACT GCAAGAGCGA CTGGAACTGG CTGAGCAAAA GCTGCAGCAG 
ACCCTGAGAA AAGCTGAGAC TTTGCCAGAG GTGGAGGCTG AGCTGGCACA GAGAGTTGCA 
GCACTTACAA AGTCTGAACT TTTGTCTCCT GGGAGCTCTG CTGCTAAAGA TGCTAAAGTT 
TTGGAACTTG CCACCAAGCT TAGGAAGGCT GAGGAGAGAC ATGGCAATAT CGAGGAGCGA 
CTGAGACAGA TGGAGGCACA GCTTGAAGAG AAGAATCAAG AACTGCAAAG GGCTAGACAG 
AGAGAGAAGA TGAATGAAGA GCATAATAAA CGTTTGTCAG AAACTGTTGA TAAGCTCCTA 
TCTGAATCCA ATGAAAGGCT CCAACTTCAT CTCAAGGAGA GGATGGCTGC CCTGGAAGAT 
AAGAATTCAC TTCTTCGAGA AATTGAAAAT GCAAAGAAAC AAATAGAAGA ACTTCAGCAT 
GAAAAGGATC AACTTGTATT AAATGTGGAT GCATTAAGGG CTGAAAATGA CCAAGTGAGG 
CTCAGAGCCA CATCACTTCA TCATAGCAGA CCAGATTTCA GATTCCCTGT GCCACCCTCC 
TCTGTGGGGG ACACTCACAC AGACTCCTAC AGCACCTCCT CTGTGCTGAG GCGGCCCCAG 
AAGGGGCGTC TGGCTGCTCT CAGGGACGAG CCTTCAAAGG TTCAGACCCT GAATGAGCAG 
GATTGGGAGC GGGCCCAGCA GGCCAGTGTT TTGGCAAATG TGGCACAAGC ATTTGAGAGT 
GATGTGGATG TGTCTGATGT GGAGGATGAC AGGGAGACCA TTTTCAGCTC AGTTGATCTG 
CTGTCACCAA GTGGTCAGGC TGATGCTCAG ACTTTGGCCA TAATGCTGCA GGAACAGTTG 
GATGCAATCA ACAAAGAGAT CAGACTGATC CAGGAGGAGA AGGAGAACAC GGAGCAGCGC 
GCGGAGGAGA TTGAGAGCCG TGTGGGCAGC GGCAGCCTGG AGGCGCACGC CCGGTTCCGC 
TCGCTGGGCT CCATCCCCCC CGCCTACGGT GGGGCCCTGG CCGGCTCCTC CCCGCCCGGC 
AGCGGCCGCT CCACACCCCG GCGCATCCCA CACAGCCCGG CCAGGGAAGT GGACAGGCTG 
GGCATCATGA CATTGCCTAG TGATTTAAGG AAACACCGTA GAAAGTCTCC AGCTTCCAGG 
GAAGAGGTAC GAGATGACAA AACCACCATA AAATGTGAGA CATCACCACC TTCATCACCT 
CGGTCTTTGC GTCTGGACAG AGTCCAGAAA GGAGCTCTGC ACACAGTGAG CCACGAAGAT 
ATCAGGGACA TTAGGAATTC AACAGGTTCA CAAGATGGGC AAACAAGTAA TCCTAGTAGC 
AGTAACAGCA GCCAAGATTC CCTTCACAAA GCCCCCAAAA AAAAGGGAAT CAAATCCTCT 
ATTGGCCGCC TGTTTGGCAA GAAGGAAAAG GGACGACCTG GACAAGGAAG CAAGGAATCT 
CTAGGACAAG GTGGCATGGG AGAAGCAGAT GGTTCCTCTC AGGATGCATT AGGTCTCAGC 
AAACTTGGAG GTCAGGCAGA GAAAAACAGG AAAATGCAGA AGAAGCACGA GTTGCTGGAG 
GAAGCCAGAA GACAGGGTTT GCCTTTTGCA CAGTGGGATG GGCCCACTGT GGTGGTGTGG 
CTGGAGCTGT GGGTGGGGAT GCCAGCCTGG TACGTGGCTG CCTGCCGAGC CAATGTGAAA 
AGCGGTGCTA TCATGTCAGC CCTGTCTGAC ACAGAAATCC AGCGGGAAAT CGGCATCAGC 
AATCCCCTGC ACAGGCTCAA GCTCCGCCTG GCCATCCAGG AAATCATGTC CCTAACCAGC 
CCCTCTGCCC CTCCCACCTC AAGGACGACC ACGGGAAATG TCTGGGTAAC ACATGAAGAG 
ATGGAAAATC TTACAGCCAC ACAACAAACG GAAGATGAGG AGGGAAGCTG GGCTCAGACA 
TTAGCCTATG GTGACATGAA CCATGAGTGG ATTGGCAATG AGTGGCTGCC AAGCCTGGGG 
CTCCCTCAGT ACCGCAGCTA CTTCATGGAA TGTCTGGTGG ATGCTCGGAT GCTGGATCAC 
TTAACTAAAA AAGACCTGCG TGGGCAGCTG AAAATGGTGG ACAGCTTTCA CAGAAACAGT 
TTTCAGTGTG GAATTATGTG CCTCCGAAGA CTGAATTACG ATCGAAAAGA GCTAGAAAGA 
AAAAGAGAAG AAAGCCAGAA TGAAATTAAG GATGTCCTTG TGTGGAGCAA TGAGAGGATG 
ATCCACTGGG TCATGTCCAT TGGCCTTAAG GAATATGCGA ACAACCTGCT CGAGAGCGGA 
GTTCACGGTG CACTTGTGGC CTTAGATGAA TCCTTTGATT ACAATGCATT AGCTCTCCTG 
CTGCAAATCC CCACTCAAAA CACACAGGCT CGTGCTGTTC TGGAGAGGGA ATTCAATAAC 
CTTCTTGCTA TGGGCACTGA CAGAAGACTT GATGAAGATG ATGATAAGAG CTTTAGGAGA 
GCACCTTCGT GGAGAAAGAA GTTTAGACCA AAGGACATAA GAGGTTTAGC TACTGGATCA 
GCAGAGACTC TTCCTGCAAA TTTCAGAGTT ACAACCTCAA TGTCTTCACC CTCTATGCAG 
CCAAAGAAGA TGCAGATTGA TGTTTACCCA CACTATTTCT ACCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PPFIA1 ( XM_015631373.2 ) cDNA ORF clone, Parus major(Great Tit) -> XP_015486859.1 Parus major PTPRF interacting protein alpha 1 (PPFIA1), transcript variant X6, mRNA.

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CloneID	OPa179673
Clone ID Related Accession (Same CDS sequence)	XM_015631373.2
Accession Version	XM_015631373.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3738bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-11-13
Organism	Parus major(Great Tit)
Product	liprin-alpha-1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_031774.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Nov 4, 2016 this sequence version replaced XM_015631373.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Parus major Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTGTG AGGTGATGCC AACCATCAGT GAGGCCGAGA TTCCCGCCGG GGGAAATGGA 
GGCCACGGTT CAGGTTCCCC GTTGCAATCA GATGCTGATT CCCACTTCGA GCAGTTAATG 
GTGTCCATGC TGGAAGAAAG GGATCGCCTC CTAGAAACGC TGCGAGAAAC TCAAGAGACG 
CTTGCCCTCA CCCAGGGCAA GCTGCATGAG GTTGGCCATG AGAGGGATTC CTTGCAGAGG 
CAGCTCAATA CTGCACTTCC ACAGGAATTT GCAGCACTCA CCAAAGAGCT GAATGTATGC 
AGGGAGCAGC TGCTTGAAAG GGAAGAAGAA ATTGCTGAGC TGAAAGCAGA AAGAAATAAT 
ACGAGGCTAT TACTGGAACA TTTGGAGTGT CTTGTCTCCA GGCATGAGCG ATCCCTCAGG 
ATGACTGTTG TGAAGAGGCA AGCTCAGTCT CCAGCAGGAG TTTCTAGTGA AGTAGAAGTT 
CTCAAAGCAC TAAAATCTTT ATTTGAGCAC CACAAAGCCC TTGATGAAAA GGTAAGGGAG 
AGATTACGAG TAGCACTTGA AAGATGTAGC TTATTGGAAG AAGAACTAGG TACTACACAT 
AAAGAGTTAA TGATTCTGAA AGAGCAAAAT AATCAGAAGA AAACACTTCC AGATGGGATG 
CTGGATATAA ATCATGAACA AGAAAATACA CCAACTACAA ATGGGAAGAG ATCCTCTGAT 
GGTTCATTAT GCCATGATGA AAACCTTGCT AAAGTGATTG AACTCCAAGA CATCATAGAT 
AAGCAAAACA AAGAGCAGAC ACAAATGAAA GAACGCCTCA CTGCTTTGTC CAGCAGAGTA 
ACAGAGCTGG AAGAAGATCT AGACACAGCA AGGAAAGACT TAATAAAATC TGAAGAAATG 
AACACAAAGT TACAGAGGGA TGTACGAGAG ACTCTGGCCC AGAAGGAGGA CATGGAGGAG 
AGAATCACCA CTCTGGAGAA ACGCTACCTC GCTGCACAAC GTGAAGCTAC ATCCGTGCAT 
GACCTCAATG ATAAACTTGA AAATGAAATT GCCACTAAAG ACTCCATGCA CCGACAGAGT 
GAAGATAAGA ACAGGCAACT GCAAGAGCGA CTGGAACTGG CTGAGCAAAA GCTGCAGCAG 
ACCCTGAGAA AAGCTGAGAC TTTGCCAGAG GTGGAGGCTG AGCTGGCACA GAGAGTTGCA 
GCACTTACAA AGTCTGAACT TTTGTCTCCT GGGAGCTCTG CTGCTAAAGA TGCTAAAGTT 
TTGGAACTTG CCACCAAGCT TAGGAAGGCT GAGGAGAGAC ATGGCAATAT CGAGGAGCGA 
CTGAGACAGA TGGAGGCACA GCTTGAAGAG AAGAATCAAG AACTGCAAAG GGCTAGACAG 
AGAGAGAAGA TGAATGAAGA GCATAATAAA CGTTTGTCAG AAACTGTTGA TAAGCTCCTA 
TCTGAATCCA ATGAAAGGCT CCAACTTCAT CTCAAGGAGA GGATGGCTGC CCTGGAAGAT 
AAGAATTCAC TTCTTCGAGA AATTGAAAAT GCAAAGAAAC AAATAGAAGA ACTTCAGCAT 
GAAAAGGATC AACTTGTATT AAATGTGGAT GCATTAAGGG CTGAAAATGA CCAAGTGAGG 
CTCAGAGCCA CATCACTTCA TCATAGCAGA CCAGATTTCA GATTCCCTGT GCCACCCTCC 
TCTGTGGGGG ACACTCACAC AGACTCCTAC AGCACCTCCT CTGTGCTGAG GCGGCCCCAG 
AAGGGGCGTC TGGCTGCTCT CAGGGACGAG CCTTCAAAGG TTCAGACCCT GAATGAGCAG 
GATTGGGAGC GGGCCCAGCA GGCCAGTGTT TTGGCAAATG TGGCACAAGC ATTTGAGAGT 
GATGTGGATG TGTCTGATGT GGAGGATGAC AGGGAGACCA TTTTCAGCTC AGTTGATCTG 
CTGTCACCAA GTGGTCAGGC TGATGCTCAG ACTTTGGCCA TAATGCTGCA GGAACAGTTG 
GATGCAATCA ACAAAGAGAT CAGACTGATC CAGGAGGAGA AGGAGAACAC GGAGCAGCGC 
GCGGAGGAGA TTGAGAGCCG TGTGGGCAGC GGCAGCCTGG AGGCGCACGC CCGGTTCCGC 
TCGCTGGGCT CCATCCCCCC CGCCTACGGT GGGGCCCTGG CCGGCTCCTC CCCGCCCGGC 
AGCGGCCGCT CCACACCCCG GCGCATCCCA CACAGCCCGG CCAGGGAAGT GGACAGGCTG 
GGCATCATGA CATTGCCTAG TGATTTAAGG AAACACCGTA GAAAGTCTCC AGCTTCCAGG 
GAAGAGGTAC GAGATGACAA AACCACCATA AAATGTGAGA CATCACCACC TTCATCACCT 
CGGTCTTTGC GTCTGGACAG AGTCCAGAAA GGAGCTCTGC ACACAGTGAG CCACGAAGAT 
ATCAGGGACA TTAGGAATTC AACAGGTTCA CAAGATGGGC AAACAAGTAA TCCTAGTAGC 
AGTAACAGCA GCCAAGATTC CCTTCACAAA GCCCCCAAAA AAAAGGGAAT CAAATCCTCT 
ATTGGCCGCC TGTTTGGCAA GAAGGAAAAG GGACGACCTG GACAAGGAAG CAAGGAATCT 
CTAGGACAAG GTGGCATGGG AGAAGCAGAT GGTTCCTCTC AGGATGCATT AGGTCTCAGC 
AAACTTGGAG GTCAGGCAGA GAAAAACAGG AAAATGCAGA AGAAGCACGA GTTGCTGGAG 
GAAGCCAGAA GACAGGGTTT GCCTTTTGCA CAGTGGGATG GGCCCACTGT GGTGGTGTGG 
CTGGAGCTGT GGGTGGGGAT GCCAGCCTGG TACGTGGCTG CCTGCCGAGC CAATGTGAAA 
AGCGGTGCTA TCATGTCAGC CCTGTCTGAC ACAGAAATCC AGCGGGAAAT CGGCATCAGC 
AATCCCCTGC ACAGGCTCAA GCTCCGCCTG GCCATCCAGG AAATCATGTC CCTAACCAGC 
CCCTCTGCCC CTCCCACCTC AAGGACGGAA GATGAGGAGG GAAGCTGGGC TCAGACATTA 
GCCTATGGTG ACATGAACCA TGAGTGGATT GGCAATGAGT GGCTGCCAAG CCTGGGGCTC 
CCTCAGTACC GCAGCTACTT CATGGAATGT CTGGTGGATG CTCGGATGCT GGATCACTTA 
ACTAAAAAAG ACCTGCGTGG GCAGCTGAAA ATGGTGGACA GCTTTCACAG AAACAGTTTT 
CAGTGTGGAA TTATGTGCCT CCGAAGACTG AATTACGATC GAAAAGAGCT AGAAAGAAAA 
AGAGAAGAAA GCCAGAATGA AATTAAGGAT GTCCTTGTGT GGAGCAATGA GAGGATGATC 
CACTGGGTCA TGTCCATTGG CCTTAAGGAA TATGCGAACA ACCTGCTCGA GAGCGGAGTT 
CACGGTGCAC TTGTGGCCTT AGATGAATCC TTTGATTACA ATGCATTAGC TCTCCTGCTG 
CAAATCCCCA CTCAAAACAC ACAGGCTCGT GCTGTTCTGG AGAGGGAATT CAATAACCTT 
CTTGCTATGG GCACTGACAG AAGACTTGAT GAAGATGATG ATAAGAGCTT TAGGAGAGCA 
CCTTCGTGGA GAAAGAAGTT TAGACCAAAG GACATAAGAG GTTTAGCTAC TGGATCAGCA 
GAGACTCTTC CTGCAAATTT CAGAGTTACA ACCTCAATGT CTTCACCCTC TATGCAGCCA 
AAGAAGATGC AGATTGATGG CAGTGTATCA GGAACACAAA GATTGGATTC TGCTACAGTA 
AGGACCTACT CCTGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015486859.1
CDS	914..4651
Translation	MMCEVMPTISEAEIPAGGNGGHGSGSPLQSDADSHFEQLMVSMLEERDRLLETLRETQETLALTQGKLHEVGHERDSLQRQLNTALPQEFAALTKELNVCREQLLEREEEIAELKAERNNTRLLLEHLECLVSRHERSLRMTVVKRQAQSPAGVSSEVEVLKALKSLFEHHKALDEKVRERLRVALERCSLLEEELGTTHKELMILKEQNNQKKTLPDGMLDINHEQENTPTTNGKRSSDGSLCHDENLAKVIELQDIIDKQNKEQTQMKERLTALSSRVTELEEDLDTARKDLIKSEEMNTKLQRDVRETLAQKEDMEERITTLEKRYLAAQREATSVHDLNDKLENEIATKDSMHRQSEDKNRQLQERLELAEQKLQQTLRKAETLPEVEAELAQRVAALTKSELLSPGSSAAKDAKVLELATKLRKAEERHGNIEERLRQMEAQLEEKNQELQRARQREKMNEEHNKRLSETVDKLLSESNERLQLHLKERMAALEDKNSLLREIENAKKQIEELQHEKDQLVLNVDALRAENDQVRLRATSLHHSRPDFRFPVPPSSVGDTHTDSYSTSSVLRRPQKGRLAALRDEPSKVQTLNEQDWERAQQASVLANVAQAFESDVDVSDVEDDRETIFSSVDLLSPSGQADAQTLAIMLQEQLDAINKEIRLIQEEKENTEQRAEEIESRVGSGSLEAHARFRSLGSIPPAYGGALAGSSPPGSGRSTPRRIPHSPAREVDRLGIMTLPSDLRKHRRKSPASREEVRDDKTTIKCETSPPSSPRSLRLDRVQKGALHTVSHEDIRDIRNSTGSQDGQTSNPSSSNSSQDSLHKAPKKKGIKSSIGRLFGKKEKGRPGQGSKESLGQGGMGEADGSSQDALGLSKLGGQAEKNRKMQKKHELLEEARRQGLPFAQWDGPTVVVWLELWVGMPAWYVAACRANVKSGAIMSALSDTEIQREIGISNPLHRLKLRLAIQEIMSLTSPSAPPTSRTEDEEGSWAQTLAYGDMNHEWIGNEWLPSLGLPQYRSYFMECLVDARMLDHLTKKDLRGQLKMVDSFHRNSFQCGIMCLRRLNYDRKELERKREESQNEIKDVLVWSNERMIHWVMSIGLKEYANNLLESGVHGALVALDESFDYNALALLLQIPTQNTQARAVLEREFNNLLAMGTDRRLDEDDDKSFRRAPSWRKKFRPKDIRGLATGSAETLPANFRVTTSMSSPSMQPKKMQIDGSVSGTQRLDSATVRTYSC

Target ORF information:

RefSeq Version	XM_015631373.2
Organism	Parus major(Great Tit)
Definition	Parus major PTPRF interacting protein alpha 1 (PPFIA1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015631373.2

ORF Insert Sequence:

ATGATGTGTG AGGTGATGCC AACCATCAGT GAGGCCGAGA TTCCCGCCGG GGGAAATGGA 
GGCCACGGTT CAGGTTCCCC GTTGCAATCA GATGCTGATT CCCACTTCGA GCAGTTAATG 
GTGTCCATGC TGGAAGAAAG GGATCGCCTC CTAGAAACGC TGCGAGAAAC TCAAGAGACG 
CTTGCCCTCA CCCAGGGCAA GCTGCATGAG GTTGGCCATG AGAGGGATTC CTTGCAGAGG 
CAGCTCAATA CTGCACTTCC ACAGGAATTT GCAGCACTCA CCAAAGAGCT GAATGTATGC 
AGGGAGCAGC TGCTTGAAAG GGAAGAAGAA ATTGCTGAGC TGAAAGCAGA AAGAAATAAT 
ACGAGGCTAT TACTGGAACA TTTGGAGTGT CTTGTCTCCA GGCATGAGCG ATCCCTCAGG 
ATGACTGTTG TGAAGAGGCA AGCTCAGTCT CCAGCAGGAG TTTCTAGTGA AGTAGAAGTT 
CTCAAAGCAC TAAAATCTTT ATTTGAGCAC CACAAAGCCC TTGATGAAAA GGTAAGGGAG 
AGATTACGAG TAGCACTTGA AAGATGTAGC TTATTGGAAG AAGAACTAGG TACTACACAT 
AAAGAGTTAA TGATTCTGAA AGAGCAAAAT AATCAGAAGA AAACACTTCC AGATGGGATG 
CTGGATATAA ATCATGAACA AGAAAATACA CCAACTACAA ATGGGAAGAG ATCCTCTGAT 
GGTTCATTAT GCCATGATGA AAACCTTGCT AAAGTGATTG AACTCCAAGA CATCATAGAT 
AAGCAAAACA AAGAGCAGAC ACAAATGAAA GAACGCCTCA CTGCTTTGTC CAGCAGAGTA 
ACAGAGCTGG AAGAAGATCT AGACACAGCA AGGAAAGACT TAATAAAATC TGAAGAAATG 
AACACAAAGT TACAGAGGGA TGTACGAGAG ACTCTGGCCC AGAAGGAGGA CATGGAGGAG 
AGAATCACCA CTCTGGAGAA ACGCTACCTC GCTGCACAAC GTGAAGCTAC ATCCGTGCAT 
GACCTCAATG ATAAACTTGA AAATGAAATT GCCACTAAAG ACTCCATGCA CCGACAGAGT 
GAAGATAAGA ACAGGCAACT GCAAGAGCGA CTGGAACTGG CTGAGCAAAA GCTGCAGCAG 
ACCCTGAGAA AAGCTGAGAC TTTGCCAGAG GTGGAGGCTG AGCTGGCACA GAGAGTTGCA 
GCACTTACAA AGTCTGAACT TTTGTCTCCT GGGAGCTCTG CTGCTAAAGA TGCTAAAGTT 
TTGGAACTTG CCACCAAGCT TAGGAAGGCT GAGGAGAGAC ATGGCAATAT CGAGGAGCGA 
CTGAGACAGA TGGAGGCACA GCTTGAAGAG AAGAATCAAG AACTGCAAAG GGCTAGACAG 
AGAGAGAAGA TGAATGAAGA GCATAATAAA CGTTTGTCAG AAACTGTTGA TAAGCTCCTA 
TCTGAATCCA ATGAAAGGCT CCAACTTCAT CTCAAGGAGA GGATGGCTGC CCTGGAAGAT 
AAGAATTCAC TTCTTCGAGA AATTGAAAAT GCAAAGAAAC AAATAGAAGA ACTTCAGCAT 
GAAAAGGATC AACTTGTATT AAATGTGGAT GCATTAAGGG CTGAAAATGA CCAAGTGAGG 
CTCAGAGCCA CATCACTTCA TCATAGCAGA CCAGATTTCA GATTCCCTGT GCCACCCTCC 
TCTGTGGGGG ACACTCACAC AGACTCCTAC AGCACCTCCT CTGTGCTGAG GCGGCCCCAG 
AAGGGGCGTC TGGCTGCTCT CAGGGACGAG CCTTCAAAGG TTCAGACCCT GAATGAGCAG 
GATTGGGAGC GGGCCCAGCA GGCCAGTGTT TTGGCAAATG TGGCACAAGC ATTTGAGAGT 
GATGTGGATG TGTCTGATGT GGAGGATGAC AGGGAGACCA TTTTCAGCTC AGTTGATCTG 
CTGTCACCAA GTGGTCAGGC TGATGCTCAG ACTTTGGCCA TAATGCTGCA GGAACAGTTG 
GATGCAATCA ACAAAGAGAT CAGACTGATC CAGGAGGAGA AGGAGAACAC GGAGCAGCGC 
GCGGAGGAGA TTGAGAGCCG TGTGGGCAGC GGCAGCCTGG AGGCGCACGC CCGGTTCCGC 
TCGCTGGGCT CCATCCCCCC CGCCTACGGT GGGGCCCTGG CCGGCTCCTC CCCGCCCGGC 
AGCGGCCGCT CCACACCCCG GCGCATCCCA CACAGCCCGG CCAGGGAAGT GGACAGGCTG 
GGCATCATGA CATTGCCTAG TGATTTAAGG AAACACCGTA GAAAGTCTCC AGCTTCCAGG 
GAAGAGGTAC GAGATGACAA AACCACCATA AAATGTGAGA CATCACCACC TTCATCACCT 
CGGTCTTTGC GTCTGGACAG AGTCCAGAAA GGAGCTCTGC ACACAGTGAG CCACGAAGAT 
ATCAGGGACA TTAGGAATTC AACAGGTTCA CAAGATGGGC AAACAAGTAA TCCTAGTAGC 
AGTAACAGCA GCCAAGATTC CCTTCACAAA GCCCCCAAAA AAAAGGGAAT CAAATCCTCT 
ATTGGCCGCC TGTTTGGCAA GAAGGAAAAG GGACGACCTG GACAAGGAAG CAAGGAATCT 
CTAGGACAAG GTGGCATGGG AGAAGCAGAT GGTTCCTCTC AGGATGCATT AGGTCTCAGC 
AAACTTGGAG GTCAGGCAGA GAAAAACAGG AAAATGCAGA AGAAGCACGA GTTGCTGGAG 
GAAGCCAGAA GACAGGGTTT GCCTTTTGCA CAGTGGGATG GGCCCACTGT GGTGGTGTGG 
CTGGAGCTGT GGGTGGGGAT GCCAGCCTGG TACGTGGCTG CCTGCCGAGC CAATGTGAAA 
AGCGGTGCTA TCATGTCAGC CCTGTCTGAC ACAGAAATCC AGCGGGAAAT CGGCATCAGC 
AATCCCCTGC ACAGGCTCAA GCTCCGCCTG GCCATCCAGG AAATCATGTC CCTAACCAGC 
CCCTCTGCCC CTCCCACCTC AAGGACGGAA GATGAGGAGG GAAGCTGGGC TCAGACATTA 
GCCTATGGTG ACATGAACCA TGAGTGGATT GGCAATGAGT GGCTGCCAAG CCTGGGGCTC 
CCTCAGTACC GCAGCTACTT CATGGAATGT CTGGTGGATG CTCGGATGCT GGATCACTTA 
ACTAAAAAAG ACCTGCGTGG GCAGCTGAAA ATGGTGGACA GCTTTCACAG AAACAGTTTT 
CAGTGTGGAA TTATGTGCCT CCGAAGACTG AATTACGATC GAAAAGAGCT AGAAAGAAAA 
AGAGAAGAAA GCCAGAATGA AATTAAGGAT GTCCTTGTGT GGAGCAATGA GAGGATGATC 
CACTGGGTCA TGTCCATTGG CCTTAAGGAA TATGCGAACA ACCTGCTCGA GAGCGGAGTT 
CACGGTGCAC TTGTGGCCTT AGATGAATCC TTTGATTACA ATGCATTAGC TCTCCTGCTG 
CAAATCCCCA CTCAAAACAC ACAGGCTCGT GCTGTTCTGG AGAGGGAATT CAATAACCTT 
CTTGCTATGG GCACTGACAG AAGACTTGAT GAAGATGATG ATAAGAGCTT TAGGAGAGCA 
CCTTCGTGGA GAAAGAAGTT TAGACCAAAG GACATAAGAG GTTTAGCTAC TGGATCAGCA 
GAGACTCTTC CTGCAAATTT CAGAGTTACA ACCTCAATGT CTTCACCCTC TATGCAGCCA 
AAGAAGATGC AGATTGATGG CAGTGTATCA GGAACACAAA GATTGGATTC TGCTACAGTA 
AGGACCTACT CCTGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PPFIA1 ( XM_015631374.2 ) cDNA ORF clone, Parus major(Great Tit) -> XP_015486860.1 Parus major PTPRF interacting protein alpha 1 (PPFIA1), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OPa179674
Clone ID Related Accession (Same CDS sequence)	XM_015631374.2
Accession Version	XM_015631374.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3726bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-11-13
Organism	Parus major(Great Tit)
Product	liprin-alpha-1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_031774.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Nov 4, 2016 this sequence version replaced XM_015631374.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Parus major Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTGTG AGGTGATGCC AACCATCAGT GAGGCCGAGA TTCCCGCCGG GGGAAATGGA 
GGCCACGGTT CAGGTTCCCC GTTGCAATCA GATGCTGATT CCCACTTCGA GCAGTTAATG 
GTGTCCATGC TGGAAGAAAG GGATCGCCTC CTAGAAACGC TGCGAGAAAC TCAAGAGACG 
CTTGCCCTCA CCCAGGGCAA GCTGCATGAG GTTGGCCATG AGAGGGATTC CTTGCAGAGG 
CAGCTCAATA CTGCACTTCC ACAGGAATTT GCAGCACTCA CCAAAGAGCT GAATGTATGC 
AGGGAGCAGC TGCTTGAAAG GGAAGAAGAA ATTGCTGAGC TGAAAGCAGA AAGAAATAAT 
ACGAGGCTAT TACTGGAACA TTTGGAGTGT CTTGTCTCCA GGCATGAGCG ATCCCTCAGG 
ATGACTGTTG TGAAGAGGCA AGCTCAGTCT CCAGCAGGAG TTTCTAGTGA AGTAGAAGTT 
CTCAAAGCAC TAAAATCTTT ATTTGAGCAC CACAAAGCCC TTGATGAAAA GGTAAGGGAG 
AGATTACGAG TAGCACTTGA AAGATGTAGC TTATTGGAAG AAGAACTAGG TACTACACAT 
AAAGAGTTAA TGATTCTGAA AGAGCAAAAT AATCAGAAGA AAACACTTCC AGATGGGATG 
CTGGATATAA ATCATGAACA AGAAAATACA CCAACTACAA ATGGGAAGAG ATCCTCTGAT 
GGTTCATTAT GCCATGATGA AAACCTTGCT AAAGTGATTG AACTCCAAGA CATCATAGAT 
AAGCAAAACA AAGAGCAGAC ACAAATGAAA GAACGCCTCA CTGCTTTGTC CAGCAGAGTA 
ACAGAGCTGG AAGAAGATCT AGACACAGCA AGGAAAGACT TAATAAAATC TGAAGAAATG 
AACACAAAGT TACAGAGGGA TGTACGAGAG ACTCTGGCCC AGAAGGAGGA CATGGAGGAG 
AGAATCACCA CTCTGGAGAA ACGCTACCTC GCTGCACAAC GTGAAGCTAC ATCCGTGCAT 
GACCTCAATG ATAAACTTGA AAATGAAATT GCCACTAAAG ACTCCATGCA CCGACAGAGT 
GAAGATAAGA ACAGGCAACT GCAAGAGCGA CTGGAACTGG CTGAGCAAAA GCTGCAGCAG 
ACCCTGAGAA AAGCTGAGAC TTTGCCAGAG GTGGAGGCTG AGCTGGCACA GAGAGTTGCA 
GCACTTACAA AGGCTGAGGA GAGACATGGC AATATCGAGG AGCGACTGAG ACAGATGGAG 
GCACAGCTTG AAGAGAAGAA TCAAGAACTG CAAAGGGCTA GACAGAGAGA GAAGATGAAT 
GAAGAGCATA ATAAACGTTT GTCAGAAACT GTTGATAAGC TCCTATCTGA ATCCAATGAA 
AGGCTCCAAC TTCATCTCAA GGAGAGGATG GCTGCCCTGG AAGATAAGAA TTCACTTCTT 
CGAGAAATTG AAAATGCAAA GAAACAAATA GAAGAACTTC AGCATGAAAA GGATCAACTT 
GTATTAAATG TGGATGCATT AAGGGCTGAA AATGACCAAG TGAGGCTCAG AGCCACATCA 
CTTCATCATA GCAGACCAGA TTTCAGATTC CCTGTGCCAC CCTCCTCTGT GGGGGACACT 
CACACAGACT CCTACAGCAC CTCCTCTGTG CTGAGGCGGC CCCAGAAGGG GCGTCTGGCT 
GCTCTCAGGG ACGAGCCTTC AAAGGTTCAG ACCCTGAATG AGCAGGATTG GGAGCGGGCC 
CAGCAGGCCA GTGTTTTGGC AAATGTGGCA CAAGCATTTG AGAGTGATGT GGATGTGTCT 
GATGTGGAGG ATGACAGGGA GACCATTTTC AGCTCAGTTG ATCTGCTGTC ACCAAGTGGT 
CAGGCTGATG CTCAGACTTT GGCCATAATG CTGCAGGAAC AGTTGGATGC AATCAACAAA 
GAGATCAGAC TGATCCAGGA GGAGAAGGAG AACACGGAGC AGCGCGCGGA GGAGATTGAG 
AGCCGTGTGG GCAGCGGCAG CCTGGAGGCG CACGCCCGGT TCCGCTCGCT GGGCTCCATC 
CCCCCCGCCT ACGGTGGGGC CCTGGCCGGC TCCTCCCCGC CCGGCAGCGG CCGCTCCACA 
CCCCGGCGCA TCCCACACAG CCCGGCCAGG GAAGTGGACA GGCTGGGCAT CATGACATTG 
CCTAGTGATT TAAGGAAACA CCGTAGAAAG TCTCCAGCTT CCAGGGAAGA GGTACGAGAT 
GACAAAACCA CCATAAAATG TGAGACATCA CCACCTTCAT CACCTCGGTC TTTGCGTCTG 
GACAGAGTCC AGAAAGGAGC TCTGCACACA GTGAGCCACG AAGATATCAG GGACATTAGG 
AATTCAACAG GTTCACAAGA TGGGCAAACA AGTAATCCTA GTAGCAGTAA CAGCAGCCAA 
GATTCCCTTC ACAAAGCCCC CAAAAAAAAG GGAATCAAAT CCTCTATTGG CCGCCTGTTT 
GGCAAGAAGG AAAAGGGACG ACCTGGACAA GGAAGCAAGG AATCTCTAGG ACAAGGTGGC 
ATGGGAGAAG CAGATGGTTC CTCTCAGGAT GCATTAGGTC TCAGCAAACT TGGAGGTCAG 
GCAGAGAAAA ACAGGAAAAT GCAGAAGAAG CACGAGTTGC TGGAGGAAGC CAGAAGACAG 
GGTTTGCCTT TTGCACAGTG GGATGGGCCC ACTGTGGTGG TGTGGCTGGA GCTGTGGGTG 
GGGATGCCAG CCTGGTACGT GGCTGCCTGC CGAGCCAATG TGAAAAGCGG TGCTATCATG 
TCAGCCCTGT CTGACACAGA AATCCAGCGG GAAATCGGCA TCAGCAATCC CCTGCACAGG 
CTCAAGCTCC GCCTGGCCAT CCAGGAAATC ATGTCCCTAA CCAGCCCCTC TGCCCCTCCC 
ACCTCAAGGA CGACCACGGG AAATGTCTGG GTAACACATG AAGAGATGGA AAATCTTACA 
GCCACACAAC AAACGGAAGA TGAGGAGGGA AGCTGGGCTC AGACATTAGC CTATGGTGAC 
ATGAACCATG AGTGGATTGG CAATGAGTGG CTGCCAAGCC TGGGGCTCCC TCAGTACCGC 
AGCTACTTCA TGGAATGTCT GGTGGATGCT CGGATGCTGG ATCACTTAAC TAAAAAAGAC 
CTGCGTGGGC AGCTGAAAAT GGTGGACAGC TTTCACAGAA ACAGTTTTCA GTGTGGAATT 
ATGTGCCTCC GAAGACTGAA TTACGATCGA AAAGAGCTAG AAAGAAAAAG AGAAGAAAGC 
CAGAATGAAA TTAAGGATGT CCTTGTGTGG AGCAATGAGA GGATGATCCA CTGGGTCATG 
TCCATTGGCC TTAAGGAATA TGCGAACAAC CTGCTCGAGA GCGGAGTTCA CGGTGCACTT 
GTGGCCTTAG ATGAATCCTT TGATTACAAT GCATTAGCTC TCCTGCTGCA AATCCCCACT 
CAAAACACAC AGGCTCGTGC TGTTCTGGAG AGGGAATTCA ATAACCTTCT TGCTATGGGC 
ACTGACAGAA GACTTGATGA AGATGATGAT AAGAGCTTTA GGAGAGCACC TTCGTGGAGA 
AAGAAGTTTA GACCAAAGGA CATAAGAGGT TTAGCTACTG GATCAGCAGA GACTCTTCCT 
GCAAATTTCA GAGTTACAAC CTCAATGTCT TCACCCTCTA TGCAGCCAAA GAAGATGCAG 
ATTGATGGCA GTGTATCAGG AACACAAAGA TTGGATTCTG CTACAGTAAG GACCTACTCC 
TGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015486860.1
CDS	913..4638
Translation	MMCEVMPTISEAEIPAGGNGGHGSGSPLQSDADSHFEQLMVSMLEERDRLLETLRETQETLALTQGKLHEVGHERDSLQRQLNTALPQEFAALTKELNVCREQLLEREEEIAELKAERNNTRLLLEHLECLVSRHERSLRMTVVKRQAQSPAGVSSEVEVLKALKSLFEHHKALDEKVRERLRVALERCSLLEEELGTTHKELMILKEQNNQKKTLPDGMLDINHEQENTPTTNGKRSSDGSLCHDENLAKVIELQDIIDKQNKEQTQMKERLTALSSRVTELEEDLDTARKDLIKSEEMNTKLQRDVRETLAQKEDMEERITTLEKRYLAAQREATSVHDLNDKLENEIATKDSMHRQSEDKNRQLQERLELAEQKLQQTLRKAETLPEVEAELAQRVAALTKAEERHGNIEERLRQMEAQLEEKNQELQRARQREKMNEEHNKRLSETVDKLLSESNERLQLHLKERMAALEDKNSLLREIENAKKQIEELQHEKDQLVLNVDALRAENDQVRLRATSLHHSRPDFRFPVPPSSVGDTHTDSYSTSSVLRRPQKGRLAALRDEPSKVQTLNEQDWERAQQASVLANVAQAFESDVDVSDVEDDRETIFSSVDLLSPSGQADAQTLAIMLQEQLDAINKEIRLIQEEKENTEQRAEEIESRVGSGSLEAHARFRSLGSIPPAYGGALAGSSPPGSGRSTPRRIPHSPAREVDRLGIMTLPSDLRKHRRKSPASREEVRDDKTTIKCETSPPSSPRSLRLDRVQKGALHTVSHEDIRDIRNSTGSQDGQTSNPSSSNSSQDSLHKAPKKKGIKSSIGRLFGKKEKGRPGQGSKESLGQGGMGEADGSSQDALGLSKLGGQAEKNRKMQKKHELLEEARRQGLPFAQWDGPTVVVWLELWVGMPAWYVAACRANVKSGAIMSALSDTEIQREIGISNPLHRLKLRLAIQEIMSLTSPSAPPTSRTTTGNVWVTHEEMENLTATQQTEDEEGSWAQTLAYGDMNHEWIGNEWLPSLGLPQYRSYFMECLVDARMLDHLTKKDLRGQLKMVDSFHRNSFQCGIMCLRRLNYDRKELERKREESQNEIKDVLVWSNERMIHWVMSIGLKEYANNLLESGVHGALVALDESFDYNALALLLQIPTQNTQARAVLEREFNNLLAMGTDRRLDEDDDKSFRRAPSWRKKFRPKDIRGLATGSAETLPANFRVTTSMSSPSMQPKKMQIDGSVSGTQRLDSATVRTYSC

Target ORF information:

RefSeq Version	XM_015631374.2
Organism	Parus major(Great Tit)
Definition	Parus major PTPRF interacting protein alpha 1 (PPFIA1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015631374.2

ORF Insert Sequence:

ATGATGTGTG AGGTGATGCC AACCATCAGT GAGGCCGAGA TTCCCGCCGG GGGAAATGGA 
GGCCACGGTT CAGGTTCCCC GTTGCAATCA GATGCTGATT CCCACTTCGA GCAGTTAATG 
GTGTCCATGC TGGAAGAAAG GGATCGCCTC CTAGAAACGC TGCGAGAAAC TCAAGAGACG 
CTTGCCCTCA CCCAGGGCAA GCTGCATGAG GTTGGCCATG AGAGGGATTC CTTGCAGAGG 
CAGCTCAATA CTGCACTTCC ACAGGAATTT GCAGCACTCA CCAAAGAGCT GAATGTATGC 
AGGGAGCAGC TGCTTGAAAG GGAAGAAGAA ATTGCTGAGC TGAAAGCAGA AAGAAATAAT 
ACGAGGCTAT TACTGGAACA TTTGGAGTGT CTTGTCTCCA GGCATGAGCG ATCCCTCAGG 
ATGACTGTTG TGAAGAGGCA AGCTCAGTCT CCAGCAGGAG TTTCTAGTGA AGTAGAAGTT 
CTCAAAGCAC TAAAATCTTT ATTTGAGCAC CACAAAGCCC TTGATGAAAA GGTAAGGGAG 
AGATTACGAG TAGCACTTGA AAGATGTAGC TTATTGGAAG AAGAACTAGG TACTACACAT 
AAAGAGTTAA TGATTCTGAA AGAGCAAAAT AATCAGAAGA AAACACTTCC AGATGGGATG 
CTGGATATAA ATCATGAACA AGAAAATACA CCAACTACAA ATGGGAAGAG ATCCTCTGAT 
GGTTCATTAT GCCATGATGA AAACCTTGCT AAAGTGATTG AACTCCAAGA CATCATAGAT 
AAGCAAAACA AAGAGCAGAC ACAAATGAAA GAACGCCTCA CTGCTTTGTC CAGCAGAGTA 
ACAGAGCTGG AAGAAGATCT AGACACAGCA AGGAAAGACT TAATAAAATC TGAAGAAATG 
AACACAAAGT TACAGAGGGA TGTACGAGAG ACTCTGGCCC AGAAGGAGGA CATGGAGGAG 
AGAATCACCA CTCTGGAGAA ACGCTACCTC GCTGCACAAC GTGAAGCTAC ATCCGTGCAT 
GACCTCAATG ATAAACTTGA AAATGAAATT GCCACTAAAG ACTCCATGCA CCGACAGAGT 
GAAGATAAGA ACAGGCAACT GCAAGAGCGA CTGGAACTGG CTGAGCAAAA GCTGCAGCAG 
ACCCTGAGAA AAGCTGAGAC TTTGCCAGAG GTGGAGGCTG AGCTGGCACA GAGAGTTGCA 
GCACTTACAA AGGCTGAGGA GAGACATGGC AATATCGAGG AGCGACTGAG ACAGATGGAG 
GCACAGCTTG AAGAGAAGAA TCAAGAACTG CAAAGGGCTA GACAGAGAGA GAAGATGAAT 
GAAGAGCATA ATAAACGTTT GTCAGAAACT GTTGATAAGC TCCTATCTGA ATCCAATGAA 
AGGCTCCAAC TTCATCTCAA GGAGAGGATG GCTGCCCTGG AAGATAAGAA TTCACTTCTT 
CGAGAAATTG AAAATGCAAA GAAACAAATA GAAGAACTTC AGCATGAAAA GGATCAACTT 
GTATTAAATG TGGATGCATT AAGGGCTGAA AATGACCAAG TGAGGCTCAG AGCCACATCA 
CTTCATCATA GCAGACCAGA TTTCAGATTC CCTGTGCCAC CCTCCTCTGT GGGGGACACT 
CACACAGACT CCTACAGCAC CTCCTCTGTG CTGAGGCGGC CCCAGAAGGG GCGTCTGGCT 
GCTCTCAGGG ACGAGCCTTC AAAGGTTCAG ACCCTGAATG AGCAGGATTG GGAGCGGGCC 
CAGCAGGCCA GTGTTTTGGC AAATGTGGCA CAAGCATTTG AGAGTGATGT GGATGTGTCT 
GATGTGGAGG ATGACAGGGA GACCATTTTC AGCTCAGTTG ATCTGCTGTC ACCAAGTGGT 
CAGGCTGATG CTCAGACTTT GGCCATAATG CTGCAGGAAC AGTTGGATGC AATCAACAAA 
GAGATCAGAC TGATCCAGGA GGAGAAGGAG AACACGGAGC AGCGCGCGGA GGAGATTGAG 
AGCCGTGTGG GCAGCGGCAG CCTGGAGGCG CACGCCCGGT TCCGCTCGCT GGGCTCCATC 
CCCCCCGCCT ACGGTGGGGC CCTGGCCGGC TCCTCCCCGC CCGGCAGCGG CCGCTCCACA 
CCCCGGCGCA TCCCACACAG CCCGGCCAGG GAAGTGGACA GGCTGGGCAT CATGACATTG 
CCTAGTGATT TAAGGAAACA CCGTAGAAAG TCTCCAGCTT CCAGGGAAGA GGTACGAGAT 
GACAAAACCA CCATAAAATG TGAGACATCA CCACCTTCAT CACCTCGGTC TTTGCGTCTG 
GACAGAGTCC AGAAAGGAGC TCTGCACACA GTGAGCCACG AAGATATCAG GGACATTAGG 
AATTCAACAG GTTCACAAGA TGGGCAAACA AGTAATCCTA GTAGCAGTAA CAGCAGCCAA 
GATTCCCTTC ACAAAGCCCC CAAAAAAAAG GGAATCAAAT CCTCTATTGG CCGCCTGTTT 
GGCAAGAAGG AAAAGGGACG ACCTGGACAA GGAAGCAAGG AATCTCTAGG ACAAGGTGGC 
ATGGGAGAAG CAGATGGTTC CTCTCAGGAT GCATTAGGTC TCAGCAAACT TGGAGGTCAG 
GCAGAGAAAA ACAGGAAAAT GCAGAAGAAG CACGAGTTGC TGGAGGAAGC CAGAAGACAG 
GGTTTGCCTT TTGCACAGTG GGATGGGCCC ACTGTGGTGG TGTGGCTGGA GCTGTGGGTG 
GGGATGCCAG CCTGGTACGT GGCTGCCTGC CGAGCCAATG TGAAAAGCGG TGCTATCATG 
TCAGCCCTGT CTGACACAGA AATCCAGCGG GAAATCGGCA TCAGCAATCC CCTGCACAGG 
CTCAAGCTCC GCCTGGCCAT CCAGGAAATC ATGTCCCTAA CCAGCCCCTC TGCCCCTCCC 
ACCTCAAGGA CGACCACGGG AAATGTCTGG GTAACACATG AAGAGATGGA AAATCTTACA 
GCCACACAAC AAACGGAAGA TGAGGAGGGA AGCTGGGCTC AGACATTAGC CTATGGTGAC 
ATGAACCATG AGTGGATTGG CAATGAGTGG CTGCCAAGCC TGGGGCTCCC TCAGTACCGC 
AGCTACTTCA TGGAATGTCT GGTGGATGCT CGGATGCTGG ATCACTTAAC TAAAAAAGAC 
CTGCGTGGGC AGCTGAAAAT GGTGGACAGC TTTCACAGAA ACAGTTTTCA GTGTGGAATT 
ATGTGCCTCC GAAGACTGAA TTACGATCGA AAAGAGCTAG AAAGAAAAAG AGAAGAAAGC 
CAGAATGAAA TTAAGGATGT CCTTGTGTGG AGCAATGAGA GGATGATCCA CTGGGTCATG 
TCCATTGGCC TTAAGGAATA TGCGAACAAC CTGCTCGAGA GCGGAGTTCA CGGTGCACTT 
GTGGCCTTAG ATGAATCCTT TGATTACAAT GCATTAGCTC TCCTGCTGCA AATCCCCACT 
CAAAACACAC AGGCTCGTGC TGTTCTGGAG AGGGAATTCA ATAACCTTCT TGCTATGGGC 
ACTGACAGAA GACTTGATGA AGATGATGAT AAGAGCTTTA GGAGAGCACC TTCGTGGAGA 
AAGAAGTTTA GACCAAAGGA CATAAGAGGT TTAGCTACTG GATCAGCAGA GACTCTTCCT 
GCAAATTTCA GAGTTACAAC CTCAATGTCT TCACCCTCTA TGCAGCCAAA GAAGATGCAG 
ATTGATGGCA GTGTATCAGG AACACAAAGA TTGGATTCTG CTACAGTAAG GACCTACTCC 
TGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PPFIA1 ( XM_015631375.2 ) cDNA ORF clone, Parus major(Great Tit) -> XP_015486861.1 Parus major PTPRF interacting protein alpha 1 (PPFIA1), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OPa179675
Clone ID Related Accession (Same CDS sequence)	XM_015631375.2
Accession Version	XM_015631375.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3711bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-11-13
Organism	Parus major(Great Tit)
Product	liprin-alpha-1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_031774.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Nov 4, 2016 this sequence version replaced XM_015631375.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Parus major Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTGTG AGGTGATGCC AACCATCAGT GAGGCCGAGA TTCCCGCCGG GGGAAATGGA 
GGCCACGGTT CAGGTTCCCC GTTGCAATCA GATGCTGATT CCCACTTCGA GCAGTTAATG 
GTGTCCATGC TGGAAGAAAG GGATCGCCTC CTAGAAACGC TGCGAGAAAC TCAAGAGACG 
CTTGCCCTCA CCCAGGGCAA GCTGCATGAG GTTGGCCATG AGAGGGATTC CTTGCAGAGG 
CAGCTCAATA CTGCACTTCC ACAGGAATTT GCAGCACTCA CCAAAGAGCT GAATGTATGC 
AGGGAGCAGC TGCTTGAAAG GGAAGAAGAA ATTGCTGAGC TGAAAGCAGA AAGAAATAAT 
ACGAGGCTAT TACTGGAACA TTTGGAGTGT CTTGTCTCCA GGCATGAGCG ATCCCTCAGG 
ATGACTGTTG TGAAGAGGCA AGCTCAGTCT CCAGCAGGAG TTTCTAGTGA AGTAGAAGTT 
CTCAAAGCAC TAAAATCTTT ATTTGAGCAC CACAAAGCCC TTGATGAAAA GGTAAGGGAG 
AGATTACGAG TAGCACTTGA AAGATGTAGC TTATTGGAAG AAGAACTAGG TACTACACAT 
AAAGAGTTAA TGATTCTGAA AGAGCAAAAT AATCAGAAGA AAACACTTCC AGATGGGATG 
CTGGATATAA ATCATGAACA AGAAAATACA CCAACTACAA ATGGGAAGAG ATCCTCTGAT 
GGTTCATTAT GCCATGATGA AAACCTTGCT AAAGTGATTG AACTCCAAGA CATCATAGAT 
AAGCAAAACA AAGAGCAGAC ACAAATGAAA GAACGCCTCA CTGCTTTGTC CAGCAGAGTA 
ACAGAGCTGG AAGAAGATCT AGACACAGCA AGGAAAGACT TAATAAAATC TGAAGAAATG 
AACACAAAGT TACAGAGGGA TGTACGAGAG ACTCTGGCCC AGAAGGAGGA CATGGAGGAG 
AGAATCACCA CTCTGGAGAA ACGCTACCTC GCTGCACAAC GTGAAGCTAC ATCCGTGCAT 
GACCTCAATG ATAAACTTGA AAATGAAATT GCCACTAAAG ACTCCATGCA CCGACAGAGT 
GAAGATAAGA ACAGGCAACT GCAAGAGCGA CTGGAACTGG CTGAGCAAAA GCTGCAGCAG 
ACCCTGAGAA AAGCTGAGAC TTTGCCAGAG GTGGAGGCTG AGCTGGCACA GAGAGTTGCA 
GCACTTACAA AGTCTGAACT TTTGTCTCCT GGGAGCTCTG CTGCTAAAGA TGCTAAAGTT 
TTGGAACTTG CCACCAAGCT TAGGAAGGCT GAGGAGAGAC ATGGCAATAT CGAGGAGCGA 
CTGAGACAGA TGGAGGCACA GCTTGAAGAG AAGAATCAAG AACTGCAAAG GGCTAGACAG 
AGAGAGAAGA TGAATGAAGA GCATAATAAA CGTTTGTCAG AAACTGTTGA TAAGCTCCTA 
TCTGAATCCA ATGAAAGGCT CCAACTTCAT CTCAAGGAGA GGATGGCTGC CCTGGAAGAT 
AAGAATTCAC TTCTTCGAGA AATTGAAAAT GCAAAGAAAC AAATAGAAGA ACTTCAGCAT 
GAAAAGGATC AACTTGTATT AAATGTGGAT GCATTAAGGG CTGAAAATGA CCAAGTGAGG 
CTCAGAGCCA CATCACTTCA TCATAGCAGA CCAGATTTCA GATTCCCTGT GCCACCCTCC 
TCTGTGGGGG ACACTCACAC AGACTCCTAC AGCACCTCCT CTGTGCTGAG GCGGCCCCAG 
AAGGGGCGTC TGGCTGCTCT CAGGGACGAG CCTTCAAAGG TTCAGACCCT GAATGAGCAG 
GATTGGGAGC GGGCCCAGCA GGCCAGTGTT TTGGCAAATG TGGCACAAGC ATTTGAGAGT 
GATGTGGATG TGTCTGATGT GGAGGATGAC AGGGAGACCA TTTTCAGCTC AGTTGATCTG 
CTGTCACCAA GTGGTCAGGC TGATGCTCAG ACTTTGGCCA TAATGCTGCA GGAACAGTTG 
GATGCAATCA ACAAAGAGAT CAGACTGATC CAGGAGGAGA AGGAGAACAC GGAGCAGCGC 
GCGGAGGAGA TTGAGAGCCG TGTGGGCAGC GGCAGCCTGG AGGCGCACGC CCGGTTCCGC 
TCGCTGGGCT CCATCCCCCC CGCCTACGGT GGGGCCCTGG CCGGCTCCTC CCCGCCCGGC 
AGCGGCCGCT CCACACCCCG GCGCATCCCA CACAGCCCGG CCAGGGAAGT GGACAGGCTG 
GGCATCATGA CATTGCCTAG TGATTTAAGG AAACACCGTA GAAAGTCTCC AGCTTCCAGG 
GAAGAGGTAC GAGATGACAA AACCACCATA AAATGTGAGA CATCACCACC TTCATCACCT 
CGGTCTTTGC GTCTGGACAG AGTCCAGAAA GGAGCTCTGC ACACAGTGAG CCACGAAGAT 
ATCAGGGACA TTAGGAATTC AACAGGTTCA CAAGATGGGC AAACAAGTAA TCCTAGTAGC 
AGTAACAGCA GCCAAGATTC CCTTCACAAA GCCCCCAAAA AAAAGGGAAT CAAATCCTCT 
ATTGGCCGCC TGTTTGGCAA GAAGGAAAAG GGACGACCTG GACAAGGAAG CAAGGAATCT 
CTAGGACAAG GTGGCATGGG AGAAGCAGAT GGTTCCTCTC AGGATGCATT AGGTCTCAGC 
AAACTTGGAG GTCAGGCAGA GAAAAACAGG AAAATGCAGA AGAAGCACGA GTTGCTGGAG 
GAAGCCAGAA GACAGGGTTT GCCTTTTGCA CAGTGGGATG GGCCCACTGT GGTGGTGTGG 
CTGGAGCTGT GGGTGGGGAT GCCAGCCTGG TACGTGGCTG CCTGCCGAGC CAATGTGAAA 
AGCGGTGCTA TCATGTCAGC CCTGTCTGAC ACAGAAATCC AGCGGGAAAT CGGCATCAGC 
AATCCCCTGC ACAGGCTCAA GCTCCGCCTG GCCATCCAGG AAATCATGTC CCTAACCAGC 
CCCTCTGCCC CTCCCACCTC AAGGACGACA TTAGCCTATG GTGACATGAA CCATGAGTGG 
ATTGGCAATG AGTGGCTGCC AAGCCTGGGG CTCCCTCAGT ACCGCAGCTA CTTCATGGAA 
TGTCTGGTGG ATGCTCGGAT GCTGGATCAC TTAACTAAAA AAGACCTGCG TGGGCAGCTG 
AAAATGGTGG ACAGCTTTCA CAGAAACAGT TTTCAGTGTG GAATTATGTG CCTCCGAAGA 
CTGAATTACG ATCGAAAAGA GCTAGAAAGA AAAAGAGAAG AAAGCCAGAA TGAAATTAAG 
GATGTCCTTG TGTGGAGCAA TGAGAGGATG ATCCACTGGG TCATGTCCAT TGGCCTTAAG 
GAATATGCGA ACAACCTGCT CGAGAGCGGA GTTCACGGTG CACTTGTGGC CTTAGATGAA 
TCCTTTGATT ACAATGCATT AGCTCTCCTG CTGCAAATCC CCACTCAAAA CACACAGGCT 
CGTGCTGTTC TGGAGAGGGA ATTCAATAAC CTTCTTGCTA TGGGCACTGA CAGAAGACTT 
GATGAAGATG ATGATAAGAG CTTTAGGAGA GCACCTTCGT GGAGAAAGAA GTTTAGACCA 
AAGGACATAA GAGGTTTAGC TACTGGATCA GCAGAGACTC TTCCTGCAAA TTTCAGAGTT 
ACAACCTCAA TGTCTTCACC CTCTATGCAG CCAAAGAAGA TGCAGATTGA TGGCAGTGTA 
TCAGGAACAC AAAGATTGGA TTCTGCTACA GTAAGGACCT ACTCCTGTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015486861.1
CDS	913..4623
Translation	MMCEVMPTISEAEIPAGGNGGHGSGSPLQSDADSHFEQLMVSMLEERDRLLETLRETQETLALTQGKLHEVGHERDSLQRQLNTALPQEFAALTKELNVCREQLLEREEEIAELKAERNNTRLLLEHLECLVSRHERSLRMTVVKRQAQSPAGVSSEVEVLKALKSLFEHHKALDEKVRERLRVALERCSLLEEELGTTHKELMILKEQNNQKKTLPDGMLDINHEQENTPTTNGKRSSDGSLCHDENLAKVIELQDIIDKQNKEQTQMKERLTALSSRVTELEEDLDTARKDLIKSEEMNTKLQRDVRETLAQKEDMEERITTLEKRYLAAQREATSVHDLNDKLENEIATKDSMHRQSEDKNRQLQERLELAEQKLQQTLRKAETLPEVEAELAQRVAALTKSELLSPGSSAAKDAKVLELATKLRKAEERHGNIEERLRQMEAQLEEKNQELQRARQREKMNEEHNKRLSETVDKLLSESNERLQLHLKERMAALEDKNSLLREIENAKKQIEELQHEKDQLVLNVDALRAENDQVRLRATSLHHSRPDFRFPVPPSSVGDTHTDSYSTSSVLRRPQKGRLAALRDEPSKVQTLNEQDWERAQQASVLANVAQAFESDVDVSDVEDDRETIFSSVDLLSPSGQADAQTLAIMLQEQLDAINKEIRLIQEEKENTEQRAEEIESRVGSGSLEAHARFRSLGSIPPAYGGALAGSSPPGSGRSTPRRIPHSPAREVDRLGIMTLPSDLRKHRRKSPASREEVRDDKTTIKCETSPPSSPRSLRLDRVQKGALHTVSHEDIRDIRNSTGSQDGQTSNPSSSNSSQDSLHKAPKKKGIKSSIGRLFGKKEKGRPGQGSKESLGQGGMGEADGSSQDALGLSKLGGQAEKNRKMQKKHELLEEARRQGLPFAQWDGPTVVVWLELWVGMPAWYVAACRANVKSGAIMSALSDTEIQREIGISNPLHRLKLRLAIQEIMSLTSPSAPPTSRTTLAYGDMNHEWIGNEWLPSLGLPQYRSYFMECLVDARMLDHLTKKDLRGQLKMVDSFHRNSFQCGIMCLRRLNYDRKELERKREESQNEIKDVLVWSNERMIHWVMSIGLKEYANNLLESGVHGALVALDESFDYNALALLLQIPTQNTQARAVLEREFNNLLAMGTDRRLDEDDDKSFRRAPSWRKKFRPKDIRGLATGSAETLPANFRVTTSMSSPSMQPKKMQIDGSVSGTQRLDSATVRTYSC

Target ORF information:

RefSeq Version	XM_015631375.2
Organism	Parus major(Great Tit)
Definition	Parus major PTPRF interacting protein alpha 1 (PPFIA1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015631375.2

ORF Insert Sequence:

ATGATGTGTG AGGTGATGCC AACCATCAGT GAGGCCGAGA TTCCCGCCGG GGGAAATGGA 
GGCCACGGTT CAGGTTCCCC GTTGCAATCA GATGCTGATT CCCACTTCGA GCAGTTAATG 
GTGTCCATGC TGGAAGAAAG GGATCGCCTC CTAGAAACGC TGCGAGAAAC TCAAGAGACG 
CTTGCCCTCA CCCAGGGCAA GCTGCATGAG GTTGGCCATG AGAGGGATTC CTTGCAGAGG 
CAGCTCAATA CTGCACTTCC ACAGGAATTT GCAGCACTCA CCAAAGAGCT GAATGTATGC 
AGGGAGCAGC TGCTTGAAAG GGAAGAAGAA ATTGCTGAGC TGAAAGCAGA AAGAAATAAT 
ACGAGGCTAT TACTGGAACA TTTGGAGTGT CTTGTCTCCA GGCATGAGCG ATCCCTCAGG 
ATGACTGTTG TGAAGAGGCA AGCTCAGTCT CCAGCAGGAG TTTCTAGTGA AGTAGAAGTT 
CTCAAAGCAC TAAAATCTTT ATTTGAGCAC CACAAAGCCC TTGATGAAAA GGTAAGGGAG 
AGATTACGAG TAGCACTTGA AAGATGTAGC TTATTGGAAG AAGAACTAGG TACTACACAT 
AAAGAGTTAA TGATTCTGAA AGAGCAAAAT AATCAGAAGA AAACACTTCC AGATGGGATG 
CTGGATATAA ATCATGAACA AGAAAATACA CCAACTACAA ATGGGAAGAG ATCCTCTGAT 
GGTTCATTAT GCCATGATGA AAACCTTGCT AAAGTGATTG AACTCCAAGA CATCATAGAT 
AAGCAAAACA AAGAGCAGAC ACAAATGAAA GAACGCCTCA CTGCTTTGTC CAGCAGAGTA 
ACAGAGCTGG AAGAAGATCT AGACACAGCA AGGAAAGACT TAATAAAATC TGAAGAAATG 
AACACAAAGT TACAGAGGGA TGTACGAGAG ACTCTGGCCC AGAAGGAGGA CATGGAGGAG 
AGAATCACCA CTCTGGAGAA ACGCTACCTC GCTGCACAAC GTGAAGCTAC ATCCGTGCAT 
GACCTCAATG ATAAACTTGA AAATGAAATT GCCACTAAAG ACTCCATGCA CCGACAGAGT 
GAAGATAAGA ACAGGCAACT GCAAGAGCGA CTGGAACTGG CTGAGCAAAA GCTGCAGCAG 
ACCCTGAGAA AAGCTGAGAC TTTGCCAGAG GTGGAGGCTG AGCTGGCACA GAGAGTTGCA 
GCACTTACAA AGTCTGAACT TTTGTCTCCT GGGAGCTCTG CTGCTAAAGA TGCTAAAGTT 
TTGGAACTTG CCACCAAGCT TAGGAAGGCT GAGGAGAGAC ATGGCAATAT CGAGGAGCGA 
CTGAGACAGA TGGAGGCACA GCTTGAAGAG AAGAATCAAG AACTGCAAAG GGCTAGACAG 
AGAGAGAAGA TGAATGAAGA GCATAATAAA CGTTTGTCAG AAACTGTTGA TAAGCTCCTA 
TCTGAATCCA ATGAAAGGCT CCAACTTCAT CTCAAGGAGA GGATGGCTGC CCTGGAAGAT 
AAGAATTCAC TTCTTCGAGA AATTGAAAAT GCAAAGAAAC AAATAGAAGA ACTTCAGCAT 
GAAAAGGATC AACTTGTATT AAATGTGGAT GCATTAAGGG CTGAAAATGA CCAAGTGAGG 
CTCAGAGCCA CATCACTTCA TCATAGCAGA CCAGATTTCA GATTCCCTGT GCCACCCTCC 
TCTGTGGGGG ACACTCACAC AGACTCCTAC AGCACCTCCT CTGTGCTGAG GCGGCCCCAG 
AAGGGGCGTC TGGCTGCTCT CAGGGACGAG CCTTCAAAGG TTCAGACCCT GAATGAGCAG 
GATTGGGAGC GGGCCCAGCA GGCCAGTGTT TTGGCAAATG TGGCACAAGC ATTTGAGAGT 
GATGTGGATG TGTCTGATGT GGAGGATGAC AGGGAGACCA TTTTCAGCTC AGTTGATCTG 
CTGTCACCAA GTGGTCAGGC TGATGCTCAG ACTTTGGCCA TAATGCTGCA GGAACAGTTG 
GATGCAATCA ACAAAGAGAT CAGACTGATC CAGGAGGAGA AGGAGAACAC GGAGCAGCGC 
GCGGAGGAGA TTGAGAGCCG TGTGGGCAGC GGCAGCCTGG AGGCGCACGC CCGGTTCCGC 
TCGCTGGGCT CCATCCCCCC CGCCTACGGT GGGGCCCTGG CCGGCTCCTC CCCGCCCGGC 
AGCGGCCGCT CCACACCCCG GCGCATCCCA CACAGCCCGG CCAGGGAAGT GGACAGGCTG 
GGCATCATGA CATTGCCTAG TGATTTAAGG AAACACCGTA GAAAGTCTCC AGCTTCCAGG 
GAAGAGGTAC GAGATGACAA AACCACCATA AAATGTGAGA CATCACCACC TTCATCACCT 
CGGTCTTTGC GTCTGGACAG AGTCCAGAAA GGAGCTCTGC ACACAGTGAG CCACGAAGAT 
ATCAGGGACA TTAGGAATTC AACAGGTTCA CAAGATGGGC AAACAAGTAA TCCTAGTAGC 
AGTAACAGCA GCCAAGATTC CCTTCACAAA GCCCCCAAAA AAAAGGGAAT CAAATCCTCT 
ATTGGCCGCC TGTTTGGCAA GAAGGAAAAG GGACGACCTG GACAAGGAAG CAAGGAATCT 
CTAGGACAAG GTGGCATGGG AGAAGCAGAT GGTTCCTCTC AGGATGCATT AGGTCTCAGC 
AAACTTGGAG GTCAGGCAGA GAAAAACAGG AAAATGCAGA AGAAGCACGA GTTGCTGGAG 
GAAGCCAGAA GACAGGGTTT GCCTTTTGCA CAGTGGGATG GGCCCACTGT GGTGGTGTGG 
CTGGAGCTGT GGGTGGGGAT GCCAGCCTGG TACGTGGCTG CCTGCCGAGC CAATGTGAAA 
AGCGGTGCTA TCATGTCAGC CCTGTCTGAC ACAGAAATCC AGCGGGAAAT CGGCATCAGC 
AATCCCCTGC ACAGGCTCAA GCTCCGCCTG GCCATCCAGG AAATCATGTC CCTAACCAGC 
CCCTCTGCCC CTCCCACCTC AAGGACGACA TTAGCCTATG GTGACATGAA CCATGAGTGG 
ATTGGCAATG AGTGGCTGCC AAGCCTGGGG CTCCCTCAGT ACCGCAGCTA CTTCATGGAA 
TGTCTGGTGG ATGCTCGGAT GCTGGATCAC TTAACTAAAA AAGACCTGCG TGGGCAGCTG 
AAAATGGTGG ACAGCTTTCA CAGAAACAGT TTTCAGTGTG GAATTATGTG CCTCCGAAGA 
CTGAATTACG ATCGAAAAGA GCTAGAAAGA AAAAGAGAAG AAAGCCAGAA TGAAATTAAG 
GATGTCCTTG TGTGGAGCAA TGAGAGGATG ATCCACTGGG TCATGTCCAT TGGCCTTAAG 
GAATATGCGA ACAACCTGCT CGAGAGCGGA GTTCACGGTG CACTTGTGGC CTTAGATGAA 
TCCTTTGATT ACAATGCATT AGCTCTCCTG CTGCAAATCC CCACTCAAAA CACACAGGCT 
CGTGCTGTTC TGGAGAGGGA ATTCAATAAC CTTCTTGCTA TGGGCACTGA CAGAAGACTT 
GATGAAGATG ATGATAAGAG CTTTAGGAGA GCACCTTCGT GGAGAAAGAA GTTTAGACCA 
AAGGACATAA GAGGTTTAGC TACTGGATCA GCAGAGACTC TTCCTGCAAA TTTCAGAGTT 
ACAACCTCAA TGTCTTCACC CTCTATGCAG CCAAAGAAGA TGCAGATTGA TGGCAGTGTA 
TCAGGAACAC AAAGATTGGA TTCTGCTACA GTAAGGACCT ACTCCTGTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PPFIA1 ( XM_015631377.2 ) cDNA ORF clone, Parus major(Great Tit) -> XP_015486863.1 Parus major PTPRF interacting protein alpha 1 (PPFIA1), transcript variant X9, mRNA.

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CloneID	OPa179676
Clone ID Related Accession (Same CDS sequence)	XM_015631377.2
Accession Version	XM_015631377.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3696bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-11-13
Organism	Parus major(Great Tit)
Product	liprin-alpha-1 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_031774.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Nov 4, 2016 this sequence version replaced XM_015631377.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Parus major Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTGTG AGGTGATGCC AACCATCAGT GAGGCCGAGA TTCCCGCCGG GGGAAATGGA 
GGCCACGGTT CAGGTTCCCC GTTGCAATCA GATGCTGATT CCCACTTCGA GCAGTTAATG 
GTGTCCATGC TGGAAGAAAG GGATCGCCTC CTAGAAACGC TGCGAGAAAC TCAAGAGACG 
CTTGCCCTCA CCCAGGGCAA GCTGCATGAG GTTGGCCATG AGAGGGATTC CTTGCAGAGG 
CAGCTCAATA CTGCACTTCC ACAGGAATTT GCAGCACTCA CCAAAGAGCT GAATGTATGC 
AGGGAGCAGC TGCTTGAAAG GGAAGAAGAA ATTGCTGAGC TGAAAGCAGA AAGAAATAAT 
ACGAGGCTAT TACTGGAACA TTTGGAGTGT CTTGTCTCCA GGCATGAGCG ATCCCTCAGG 
ATGACTGTTG TGAAGAGGCA AGCTCAGTCT CCAGCAGGAG TTTCTAGTGA AGTAGAAGTT 
CTCAAAGCAC TAAAATCTTT ATTTGAGCAC CACAAAGCCC TTGATGAAAA GGTAAGGGAG 
AGATTACGAG TAGCACTTGA AAGATGTAGC TTATTGGAAG AAGAACTAGG TACTACACAT 
AAAGAGTTAA TGATTCTGAA AGAGCAAAAT AATCAGAAGA AAACACTTCC AGATGGGATG 
CTGGATATAA ATCATGAACA AGAAAATACA CCAACTACAA ATGGGAAGAG ATCCTCTGAT 
GGTTCATTAT GCCATGATGA AAACCTTGCT AAAGTGATTG AACTCCAAGA CATCATAGAT 
AAGCAAAACA AAGAGCAGAC ACAAATGAAA GAACGCCTCA CTGCTTTGTC CAGCAGAGTA 
ACAGAGCTGG AAGAAGATCT AGACACAGCA AGGAAAGACT TAATAAAATC TGAAGAAATG 
AACACAAAGT TACAGAGGGA TGTACGAGAG ACTCTGGCCC AGAAGGAGGA CATGGAGGAG 
AGAATCACCA CTCTGGAGAA ACGCTACCTC GCTGCACAAC GTGAAGCTAC ATCCGTGCAT 
GACCTCAATG ATAAACTTGA AAATGAAATT GCCACTAAAG ACTCCATGCA CCGACAGAGT 
GAAGATAAGA ACAGGCAACT GCAAGAGCGA CTGGAACTGG CTGAGCAAAA GCTGCAGCAG 
ACCCTGAGAA AAGCTGAGAC TTTGCCAGAG GTGGAGGCTG AGCTGGCACA GAGAGTTGCA 
GCACTTACAA AGGCTGAGGA GAGACATGGC AATATCGAGG AGCGACTGAG ACAGATGGAG 
GCACAGCTTG AAGAGAAGAA TCAAGAACTG CAAAGGGCTA GACAGAGAGA GAAGATGAAT 
GAAGAGCATA ATAAACGTTT GTCAGAAACT GTTGATAAGC TCCTATCTGA ATCCAATGAA 
AGGCTCCAAC TTCATCTCAA GGAGAGGATG GCTGCCCTGG AAGATAAGAA TTCACTTCTT 
CGAGAAATTG AAAATGCAAA GAAACAAATA GAAGAACTTC AGCATGAAAA GGATCAACTT 
GTATTAAATG TGGATGCATT AAGGGCTGAA AATGACCAAG TGAGGCTCAG AGCCACATCA 
CTTCATCATA GCAGACCAGA TTTCAGATTC CCTGTGCCAC CCTCCTCTGT GGGGGACACT 
CACACAGACT CCTACAGCAC CTCCTCTGTG CTGAGGCGGC CCCAGAAGGG GCGTCTGGCT 
GCTCTCAGGG ACGAGCCTTC AAAGGTTCAG ACCCTGAATG AGCAGGATTG GGAGCGGGCC 
CAGCAGGCCA GTGTTTTGGC AAATGTGGCA CAAGCATTTG AGAGTGATGT GGATGTGTCT 
GATGTGGAGG ATGACAGGGA GACCATTTTC AGCTCAGTTG ATCTGCTGTC ACCAAGTGGT 
CAGGCTGATG CTCAGACTTT GGCCATAATG CTGCAGGAAC AGTTGGATGC AATCAACAAA 
GAGATCAGAC TGATCCAGGA GGAGAAGGAG AACACGGAGC AGCGCGCGGA GGAGATTGAG 
AGCCGTGTGG GCAGCGGCAG CCTGGAGGCG CACGCCCGGT TCCGCTCGCT GGGCTCCATC 
CCCCCCGCCT ACGGTGGGGC CCTGGCCGGC TCCTCCCCGC CCGGCAGCGG CCGCTCCACA 
CCCCGGCGCA TCCCACACAG CCCGGCCAGG GAAGTGGACA GGCTGGGCAT CATGACATTG 
TCTCCAGCTT CCAGGGAAGA GGTACGAGAT GACAAAACCA CCATAAAATG TGAGACATCA 
CCACCTTCAT CACCTCGGTC TTTGCGTCTG GACAGAGTCC AGAAAGGAGC TCTGCACACA 
GTGAGCCACG AAGATATCAG GGACATTAGG AATTCAACAG GTTCACAAGA TGGGCAAACA 
AGTAATCCTA GTAGCAGTAA CAGCAGCCAA GATTCCCTTC ACAAAGCCCC CAAAAAAAAG 
GGAATCAAAT CCTCTATTGG CCGCCTGTTT GGCAAGAAGG AAAAGGGACG ACCTGGACAA 
GGAAGCAAGG AATCTCTAGG ACAAGGTGGC ATGGGAGAAG CAGATGGTTC CTCTCAGGAT 
GCATTAGGTC TCAGCAAACT TGGAGGTCAG GCAGAGAAAA ACAGGAAAAT GCAGAAGAAG 
CACGAGTTGC TGGAGGAAGC CAGAAGACAG GGTTTGCCTT TTGCACAGTG GGATGGGCCC 
ACTGTGGTGG TGTGGCTGGA GCTGTGGGTG GGGATGCCAG CCTGGTACGT GGCTGCCTGC 
CGAGCCAATG TGAAAAGCGG TGCTATCATG TCAGCCCTGT CTGACACAGA AATCCAGCGG 
GAAATCGGCA TCAGCAATCC CCTGCACAGG CTCAAGCTCC GCCTGGCCAT CCAGGAAATC 
ATGTCCCTAA CCAGCCCCTC TGCCCCTCCC ACCTCAAGGA CGACCACGGG AAATGTCTGG 
GTAACACATG AAGAGATGGA AAATCTTACA GCCACACAAC AAACGGAAGA TGAGGAGGGA 
AGCTGGGCTC AGACATTAGC CTATGGTGAC ATGAACCATG AGTGGATTGG CAATGAGTGG 
CTGCCAAGCC TGGGGCTCCC TCAGTACCGC AGCTACTTCA TGGAATGTCT GGTGGATGCT 
CGGATGCTGG ATCACTTAAC TAAAAAAGAC CTGCGTGGGC AGCTGAAAAT GGTGGACAGC 
TTTCACAGAA ACAGTTTTCA GTGTGGAATT ATGTGCCTCC GAAGACTGAA TTACGATCGA 
AAAGAGCTAG AAAGAAAAAG AGAAGAAAGC CAGAATGAAA TTAAGGATGT CCTTGTGTGG 
AGCAATGAGA GGATGATCCA CTGGGTCATG TCCATTGGCC TTAAGGAATA TGCGAACAAC 
CTGCTCGAGA GCGGAGTTCA CGGTGCACTT GTGGCCTTAG ATGAATCCTT TGATTACAAT 
GCATTAGCTC TCCTGCTGCA AATCCCCACT CAAAACACAC AGGCTCGTGC TGTTCTGGAG 
AGGGAATTCA ATAACCTTCT TGCTATGGGC ACTGACAGAA GACTTGATGA AGATGATGAT 
AAGAGCTTTA GGAGAGCACC TTCGTGGAGA AAGAAGTTTA GACCAAAGGA CATAAGAGGT 
TTAGCTACTG GATCAGCAGA GACTCTTCCT GCAAATTTCA GAGTTACAAC CTCAATGTCT 
TCACCCTCTA TGCAGCCAAA GAAGATGCAG ATTGATGGCA GTGTATCAGG AACACAAAGA 
TTGGATTCTG CTACAGTAAG GACCTACTCC TGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015486863.1
CDS	913..4608
Translation	MMCEVMPTISEAEIPAGGNGGHGSGSPLQSDADSHFEQLMVSMLEERDRLLETLRETQETLALTQGKLHEVGHERDSLQRQLNTALPQEFAALTKELNVCREQLLEREEEIAELKAERNNTRLLLEHLECLVSRHERSLRMTVVKRQAQSPAGVSSEVEVLKALKSLFEHHKALDEKVRERLRVALERCSLLEEELGTTHKELMILKEQNNQKKTLPDGMLDINHEQENTPTTNGKRSSDGSLCHDENLAKVIELQDIIDKQNKEQTQMKERLTALSSRVTELEEDLDTARKDLIKSEEMNTKLQRDVRETLAQKEDMEERITTLEKRYLAAQREATSVHDLNDKLENEIATKDSMHRQSEDKNRQLQERLELAEQKLQQTLRKAETLPEVEAELAQRVAALTKAEERHGNIEERLRQMEAQLEEKNQELQRARQREKMNEEHNKRLSETVDKLLSESNERLQLHLKERMAALEDKNSLLREIENAKKQIEELQHEKDQLVLNVDALRAENDQVRLRATSLHHSRPDFRFPVPPSSVGDTHTDSYSTSSVLRRPQKGRLAALRDEPSKVQTLNEQDWERAQQASVLANVAQAFESDVDVSDVEDDRETIFSSVDLLSPSGQADAQTLAIMLQEQLDAINKEIRLIQEEKENTEQRAEEIESRVGSGSLEAHARFRSLGSIPPAYGGALAGSSPPGSGRSTPRRIPHSPAREVDRLGIMTLSPASREEVRDDKTTIKCETSPPSSPRSLRLDRVQKGALHTVSHEDIRDIRNSTGSQDGQTSNPSSSNSSQDSLHKAPKKKGIKSSIGRLFGKKEKGRPGQGSKESLGQGGMGEADGSSQDALGLSKLGGQAEKNRKMQKKHELLEEARRQGLPFAQWDGPTVVVWLELWVGMPAWYVAACRANVKSGAIMSALSDTEIQREIGISNPLHRLKLRLAIQEIMSLTSPSAPPTSRTTTGNVWVTHEEMENLTATQQTEDEEGSWAQTLAYGDMNHEWIGNEWLPSLGLPQYRSYFMECLVDARMLDHLTKKDLRGQLKMVDSFHRNSFQCGIMCLRRLNYDRKELERKREESQNEIKDVLVWSNERMIHWVMSIGLKEYANNLLESGVHGALVALDESFDYNALALLLQIPTQNTQARAVLEREFNNLLAMGTDRRLDEDDDKSFRRAPSWRKKFRPKDIRGLATGSAETLPANFRVTTSMSSPSMQPKKMQIDGSVSGTQRLDSATVRTYSC

Target ORF information:

RefSeq Version	XM_015631377.2
Organism	Parus major(Great Tit)
Definition	Parus major PTPRF interacting protein alpha 1 (PPFIA1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015631377.2

ORF Insert Sequence:

ATGATGTGTG AGGTGATGCC AACCATCAGT GAGGCCGAGA TTCCCGCCGG GGGAAATGGA 
GGCCACGGTT CAGGTTCCCC GTTGCAATCA GATGCTGATT CCCACTTCGA GCAGTTAATG 
GTGTCCATGC TGGAAGAAAG GGATCGCCTC CTAGAAACGC TGCGAGAAAC TCAAGAGACG 
CTTGCCCTCA CCCAGGGCAA GCTGCATGAG GTTGGCCATG AGAGGGATTC CTTGCAGAGG 
CAGCTCAATA CTGCACTTCC ACAGGAATTT GCAGCACTCA CCAAAGAGCT GAATGTATGC 
AGGGAGCAGC TGCTTGAAAG GGAAGAAGAA ATTGCTGAGC TGAAAGCAGA AAGAAATAAT 
ACGAGGCTAT TACTGGAACA TTTGGAGTGT CTTGTCTCCA GGCATGAGCG ATCCCTCAGG 
ATGACTGTTG TGAAGAGGCA AGCTCAGTCT CCAGCAGGAG TTTCTAGTGA AGTAGAAGTT 
CTCAAAGCAC TAAAATCTTT ATTTGAGCAC CACAAAGCCC TTGATGAAAA GGTAAGGGAG 
AGATTACGAG TAGCACTTGA AAGATGTAGC TTATTGGAAG AAGAACTAGG TACTACACAT 
AAAGAGTTAA TGATTCTGAA AGAGCAAAAT AATCAGAAGA AAACACTTCC AGATGGGATG 
CTGGATATAA ATCATGAACA AGAAAATACA CCAACTACAA ATGGGAAGAG ATCCTCTGAT 
GGTTCATTAT GCCATGATGA AAACCTTGCT AAAGTGATTG AACTCCAAGA CATCATAGAT 
AAGCAAAACA AAGAGCAGAC ACAAATGAAA GAACGCCTCA CTGCTTTGTC CAGCAGAGTA 
ACAGAGCTGG AAGAAGATCT AGACACAGCA AGGAAAGACT TAATAAAATC TGAAGAAATG 
AACACAAAGT TACAGAGGGA TGTACGAGAG ACTCTGGCCC AGAAGGAGGA CATGGAGGAG 
AGAATCACCA CTCTGGAGAA ACGCTACCTC GCTGCACAAC GTGAAGCTAC ATCCGTGCAT 
GACCTCAATG ATAAACTTGA AAATGAAATT GCCACTAAAG ACTCCATGCA CCGACAGAGT 
GAAGATAAGA ACAGGCAACT GCAAGAGCGA CTGGAACTGG CTGAGCAAAA GCTGCAGCAG 
ACCCTGAGAA AAGCTGAGAC TTTGCCAGAG GTGGAGGCTG AGCTGGCACA GAGAGTTGCA 
GCACTTACAA AGGCTGAGGA GAGACATGGC AATATCGAGG AGCGACTGAG ACAGATGGAG 
GCACAGCTTG AAGAGAAGAA TCAAGAACTG CAAAGGGCTA GACAGAGAGA GAAGATGAAT 
GAAGAGCATA ATAAACGTTT GTCAGAAACT GTTGATAAGC TCCTATCTGA ATCCAATGAA 
AGGCTCCAAC TTCATCTCAA GGAGAGGATG GCTGCCCTGG AAGATAAGAA TTCACTTCTT 
CGAGAAATTG AAAATGCAAA GAAACAAATA GAAGAACTTC AGCATGAAAA GGATCAACTT 
GTATTAAATG TGGATGCATT AAGGGCTGAA AATGACCAAG TGAGGCTCAG AGCCACATCA 
CTTCATCATA GCAGACCAGA TTTCAGATTC CCTGTGCCAC CCTCCTCTGT GGGGGACACT 
CACACAGACT CCTACAGCAC CTCCTCTGTG CTGAGGCGGC CCCAGAAGGG GCGTCTGGCT 
GCTCTCAGGG ACGAGCCTTC AAAGGTTCAG ACCCTGAATG AGCAGGATTG GGAGCGGGCC 
CAGCAGGCCA GTGTTTTGGC AAATGTGGCA CAAGCATTTG AGAGTGATGT GGATGTGTCT 
GATGTGGAGG ATGACAGGGA GACCATTTTC AGCTCAGTTG ATCTGCTGTC ACCAAGTGGT 
CAGGCTGATG CTCAGACTTT GGCCATAATG CTGCAGGAAC AGTTGGATGC AATCAACAAA 
GAGATCAGAC TGATCCAGGA GGAGAAGGAG AACACGGAGC AGCGCGCGGA GGAGATTGAG 
AGCCGTGTGG GCAGCGGCAG CCTGGAGGCG CACGCCCGGT TCCGCTCGCT GGGCTCCATC 
CCCCCCGCCT ACGGTGGGGC CCTGGCCGGC TCCTCCCCGC CCGGCAGCGG CCGCTCCACA 
CCCCGGCGCA TCCCACACAG CCCGGCCAGG GAAGTGGACA GGCTGGGCAT CATGACATTG 
TCTCCAGCTT CCAGGGAAGA GGTACGAGAT GACAAAACCA CCATAAAATG TGAGACATCA 
CCACCTTCAT CACCTCGGTC TTTGCGTCTG GACAGAGTCC AGAAAGGAGC TCTGCACACA 
GTGAGCCACG AAGATATCAG GGACATTAGG AATTCAACAG GTTCACAAGA TGGGCAAACA 
AGTAATCCTA GTAGCAGTAA CAGCAGCCAA GATTCCCTTC ACAAAGCCCC CAAAAAAAAG 
GGAATCAAAT CCTCTATTGG CCGCCTGTTT GGCAAGAAGG AAAAGGGACG ACCTGGACAA 
GGAAGCAAGG AATCTCTAGG ACAAGGTGGC ATGGGAGAAG CAGATGGTTC CTCTCAGGAT 
GCATTAGGTC TCAGCAAACT TGGAGGTCAG GCAGAGAAAA ACAGGAAAAT GCAGAAGAAG 
CACGAGTTGC TGGAGGAAGC CAGAAGACAG GGTTTGCCTT TTGCACAGTG GGATGGGCCC 
ACTGTGGTGG TGTGGCTGGA GCTGTGGGTG GGGATGCCAG CCTGGTACGT GGCTGCCTGC 
CGAGCCAATG TGAAAAGCGG TGCTATCATG TCAGCCCTGT CTGACACAGA AATCCAGCGG 
GAAATCGGCA TCAGCAATCC CCTGCACAGG CTCAAGCTCC GCCTGGCCAT CCAGGAAATC 
ATGTCCCTAA CCAGCCCCTC TGCCCCTCCC ACCTCAAGGA CGACCACGGG AAATGTCTGG 
GTAACACATG AAGAGATGGA AAATCTTACA GCCACACAAC AAACGGAAGA TGAGGAGGGA 
AGCTGGGCTC AGACATTAGC CTATGGTGAC ATGAACCATG AGTGGATTGG CAATGAGTGG 
CTGCCAAGCC TGGGGCTCCC TCAGTACCGC AGCTACTTCA TGGAATGTCT GGTGGATGCT 
CGGATGCTGG ATCACTTAAC TAAAAAAGAC CTGCGTGGGC AGCTGAAAAT GGTGGACAGC 
TTTCACAGAA ACAGTTTTCA GTGTGGAATT ATGTGCCTCC GAAGACTGAA TTACGATCGA 
AAAGAGCTAG AAAGAAAAAG AGAAGAAAGC CAGAATGAAA TTAAGGATGT CCTTGTGTGG 
AGCAATGAGA GGATGATCCA CTGGGTCATG TCCATTGGCC TTAAGGAATA TGCGAACAAC 
CTGCTCGAGA GCGGAGTTCA CGGTGCACTT GTGGCCTTAG ATGAATCCTT TGATTACAAT 
GCATTAGCTC TCCTGCTGCA AATCCCCACT CAAAACACAC AGGCTCGTGC TGTTCTGGAG 
AGGGAATTCA ATAACCTTCT TGCTATGGGC ACTGACAGAA GACTTGATGA AGATGATGAT 
AAGAGCTTTA GGAGAGCACC TTCGTGGAGA AAGAAGTTTA GACCAAAGGA CATAAGAGGT 
TTAGCTACTG GATCAGCAGA GACTCTTCCT GCAAATTTCA GAGTTACAAC CTCAATGTCT 
TCACCCTCTA TGCAGCCAAA GAAGATGCAG ATTGATGGCA GTGTATCAGG AACACAAAGA 
TTGGATTCTG CTACAGTAAG GACCTACTCC TGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PPFIA1 ( XM_015631378.2 ) cDNA ORF clone, Parus major(Great Tit) -> XP_015486864.1 Parus major PTPRF interacting protein alpha 1 (PPFIA1), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OPa179677
Clone ID Related Accession (Same CDS sequence)	XM_015631378.2
Accession Version	XM_015631378.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3669bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-11-13
Organism	Parus major(Great Tit)
Product	liprin-alpha-1 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_031774.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Nov 4, 2016 this sequence version replaced XM_015631378.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Parus major Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTGTG AGGTGATGCC AACCATCAGT GAGGCCGAGA TTCCCGCCGG GGGAAATGGA 
GGCCACGGTT CAGGTTCCCC GTTGCAATCA GATGCTGATT CCCACTTCGA GCAGTTAATG 
GTGTCCATGC TGGAAGAAAG GGATCGCCTC CTAGAAACGC TGCGAGAAAC TCAAGAGACG 
CTTGCCCTCA CCCAGGGCAA GCTGCATGAG GTTGGCCATG AGAGGGATTC CTTGCAGAGG 
CAGCTCAATA CTGCACTTCC ACAGGAATTT GCAGCACTCA CCAAAGAGCT GAATGTATGC 
AGGGAGCAGC TGCTTGAAAG GGAAGAAGAA ATTGCTGAGC TGAAAGCAGA AAGAAATAAT 
ACGAGGCTAT TACTGGAACA TTTGGAGTGT CTTGTCTCCA GGCATGAGCG ATCCCTCAGG 
ATGACTGTTG TGAAGAGGCA AGCTCAGTCT CCAGCAGGAG TTTCTAGTGA AGTAGAAGTT 
CTCAAAGCAC TAAAATCTTT ATTTGAGCAC CACAAAGCCC TTGATGAAAA GGTAAGGGAG 
AGATTACGAG TAGCACTTGA AAGATGTAGC TTATTGGAAG AAGAACTAGG TACTACACAT 
AAAGAGTTAA TGATTCTGAA AGAGCAAAAT AATCAGAAGA AAACACTTCC AGATGGGATG 
CTGGATATAA ATCATGAACA AGAAAATACA CCAACTACAA ATGGGAAGAG ATCCTCTGAT 
GGTTCATTAT GCCATGATGA AAACCTTGCT AAAGTGATTG AACTCCAAGA CATCATAGAT 
AAGCAAAACA AAGAGCAGAC ACAAATGAAA GAACGCCTCA CTGCTTTGTC CAGCAGAGTA 
ACAGAGCTGG AAGAAGATCT AGACACAGCA AGGAAAGACT TAATAAAATC TGAAGAAATG 
AACACAAAGT TACAGAGGGA TGTACGAGAG ACTCTGGCCC AGAAGGAGGA CATGGAGGAG 
AGAATCACCA CTCTGGAGAA ACGCTACCTC GCTGCACAAC GTGAAGCTAC ATCCGTGCAT 
GACCTCAATG ATAAACTTGA AAATGAAATT GCCACTAAAG ACTCCATGCA CCGACAGAGT 
GAAGATAAGA ACAGGCAACT GCAAGAGCGA CTGGAACTGG CTGAGCAAAA GCTGCAGCAG 
ACCCTGAGAA AAGCTGAGAC TTTGCCAGAG GTGGAGGCTG AGCTGGCACA GAGAGTTGCA 
GCACTTACAA AGGCTGAGGA GAGACATGGC AATATCGAGG AGCGACTGAG ACAGATGGAG 
GCACAGCTTG AAGAGAAGAA TCAAGAACTG CAAAGGGCTA GACAGAGAGA GAAGATGAAT 
GAAGAGCATA ATAAACGTTT GTCAGAAACT GTTGATAAGC TCCTATCTGA ATCCAATGAA 
AGGCTCCAAC TTCATCTCAA GGAGAGGATG GCTGCCCTGG AAGATAAGAA TTCACTTCTT 
CGAGAAATTG AAAATGCAAA GAAACAAATA GAAGAACTTC AGCATGAAAA GGATCAACTT 
GTATTAAATG TGGATGCATT AAGGGCTGAA AATGACCAAG TGAGGCTCAG AGCCACATCA 
CTTCATCATA GCAGACCAGA TTTCAGATTC CCTGTGCCAC CCTCCTCTGT GGGGGACACT 
CACACAGACT CCTACAGCAC CTCCTCTGTG CTGAGGCGGC CCCAGAAGGG GCGTCTGGCT 
GCTCTCAGGG ACGAGCCTTC AAAGGTTCAG ACCCTGAATG AGCAGGATTG GGAGCGGGCC 
CAGCAGGCCA GTGTTTTGGC AAATGTGGCA CAAGCATTTG AGAGTGATGT GGATGTGTCT 
GATGTGGAGG ATGACAGGGA GACCATTTTC AGCTCAGTTG ATCTGCTGTC ACCAAGTGGT 
CAGGCTGATG CTCAGACTTT GGCCATAATG CTGCAGGAAC AGTTGGATGC AATCAACAAA 
GAGATCAGAC TGATCCAGGA GGAGAAGGAG AACACGGAGC AGCGCGCGGA GGAGATTGAG 
AGCCGTGTGG GCAGCGGCAG CCTGGAGGCG CACGCCCGGT TCCGCTCGCT GGGCTCCATC 
CCCCCCGCCT ACGGTGGGGC CCTGGCCGGC TCCTCCCCGC CCGGCAGCGG CCGCTCCACA 
CCCCGGCGCA TCCCACACAG CCCGGCCAGG GAAGTGGACA GGCTGGGCAT CATGACATTG 
TCTCCAGCTT CCAGGGAAGA GGTACGAGAT GACAAAACCA CCATAAAATG TGAGACATCA 
CCACCTTCAT CACCTCGGTC TTTGCGTCTG GACAGAGTCC AGAAAGGAGC TCTGCACACA 
GTGAGCCACG AAGATATCAG GGACATTAGG AATTCAACAG GTTCACAAGA TGGGCAAACA 
AGTAATCCTA GTAGCAGTAA CAGCAGCCAA GATTCCCTTC ACAAAGCCCC CAAAAAAAAG 
GGAATCAAAT CCTCTATTGG CCGCCTGTTT GGCAAGAAGG AAAAGGGACG ACCTGGACAA 
GGAAGCAAGG AATCTCTAGG ACAAGGTGGC ATGGGAGAAG CAGATGGTTC CTCTCAGGAT 
GCATTAGGTC TCAGCAAACT TGGAGGTCAG GCAGAGAAAA ACAGGAAAAT GCAGAAGAAG 
CACGAGTTGC TGGAGGAAGC CAGAAGACAG GGTTTGCCTT TTGCACAGTG GGATGGGCCC 
ACTGTGGTGG TGTGGCTGGA GCTGTGGGTG GGGATGCCAG CCTGGTACGT GGCTGCCTGC 
CGAGCCAATG TGAAAAGCGG TGCTATCATG TCAGCCCTGT CTGACACAGA AATCCAGCGG 
GAAATCGGCA TCAGCAATCC CCTGCACAGG CTCAAGCTCC GCCTGGCCAT CCAGGAAATC 
ATGTCCCTAA CCAGCCCCTC TGCCCCTCCC ACCTCAAGGA CGACCACGGG AAATGTCTGG 
GTAACACATG AAGAGATGGA AAATCTTACA GCCACACAAC AAACGACATT AGCCTATGGT 
GACATGAACC ATGAGTGGAT TGGCAATGAG TGGCTGCCAA GCCTGGGGCT CCCTCAGTAC 
CGCAGCTACT TCATGGAATG TCTGGTGGAT GCTCGGATGC TGGATCACTT AACTAAAAAA 
GACCTGCGTG GGCAGCTGAA AATGGTGGAC AGCTTTCACA GAAACAGTTT TCAGTGTGGA 
ATTATGTGCC TCCGAAGACT GAATTACGAT CGAAAAGAGC TAGAAAGAAA AAGAGAAGAA 
AGCCAGAATG AAATTAAGGA TGTCCTTGTG TGGAGCAATG AGAGGATGAT CCACTGGGTC 
ATGTCCATTG GCCTTAAGGA ATATGCGAAC AACCTGCTCG AGAGCGGAGT TCACGGTGCA 
CTTGTGGCCT TAGATGAATC CTTTGATTAC AATGCATTAG CTCTCCTGCT GCAAATCCCC 
ACTCAAAACA CACAGGCTCG TGCTGTTCTG GAGAGGGAAT TCAATAACCT TCTTGCTATG 
GGCACTGACA GAAGACTTGA TGAAGATGAT GATAAGAGCT TTAGGAGAGC ACCTTCGTGG 
AGAAAGAAGT TTAGACCAAA GGACATAAGA GGTTTAGCTA CTGGATCAGC AGAGACTCTT 
CCTGCAAATT TCAGAGTTAC AACCTCAATG TCTTCACCCT CTATGCAGCC AAAGAAGATG 
CAGATTGATG GCAGTGTATC AGGAACACAA AGATTGGATT CTGCTACAGT AAGGACCTAC 
TCCTGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015486864.1
CDS	912..4580
Translation	MMCEVMPTISEAEIPAGGNGGHGSGSPLQSDADSHFEQLMVSMLEERDRLLETLRETQETLALTQGKLHEVGHERDSLQRQLNTALPQEFAALTKELNVCREQLLEREEEIAELKAERNNTRLLLEHLECLVSRHERSLRMTVVKRQAQSPAGVSSEVEVLKALKSLFEHHKALDEKVRERLRVALERCSLLEEELGTTHKELMILKEQNNQKKTLPDGMLDINHEQENTPTTNGKRSSDGSLCHDENLAKVIELQDIIDKQNKEQTQMKERLTALSSRVTELEEDLDTARKDLIKSEEMNTKLQRDVRETLAQKEDMEERITTLEKRYLAAQREATSVHDLNDKLENEIATKDSMHRQSEDKNRQLQERLELAEQKLQQTLRKAETLPEVEAELAQRVAALTKAEERHGNIEERLRQMEAQLEEKNQELQRARQREKMNEEHNKRLSETVDKLLSESNERLQLHLKERMAALEDKNSLLREIENAKKQIEELQHEKDQLVLNVDALRAENDQVRLRATSLHHSRPDFRFPVPPSSVGDTHTDSYSTSSVLRRPQKGRLAALRDEPSKVQTLNEQDWERAQQASVLANVAQAFESDVDVSDVEDDRETIFSSVDLLSPSGQADAQTLAIMLQEQLDAINKEIRLIQEEKENTEQRAEEIESRVGSGSLEAHARFRSLGSIPPAYGGALAGSSPPGSGRSTPRRIPHSPAREVDRLGIMTLSPASREEVRDDKTTIKCETSPPSSPRSLRLDRVQKGALHTVSHEDIRDIRNSTGSQDGQTSNPSSSNSSQDSLHKAPKKKGIKSSIGRLFGKKEKGRPGQGSKESLGQGGMGEADGSSQDALGLSKLGGQAEKNRKMQKKHELLEEARRQGLPFAQWDGPTVVVWLELWVGMPAWYVAACRANVKSGAIMSALSDTEIQREIGISNPLHRLKLRLAIQEIMSLTSPSAPPTSRTTTGNVWVTHEEMENLTATQQTTLAYGDMNHEWIGNEWLPSLGLPQYRSYFMECLVDARMLDHLTKKDLRGQLKMVDSFHRNSFQCGIMCLRRLNYDRKELERKREESQNEIKDVLVWSNERMIHWVMSIGLKEYANNLLESGVHGALVALDESFDYNALALLLQIPTQNTQARAVLEREFNNLLAMGTDRRLDEDDDKSFRRAPSWRKKFRPKDIRGLATGSAETLPANFRVTTSMSSPSMQPKKMQIDGSVSGTQRLDSATVRTYSC

Target ORF information:

RefSeq Version	XM_015631378.2
Organism	Parus major(Great Tit)
Definition	Parus major PTPRF interacting protein alpha 1 (PPFIA1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015631378.2

ORF Insert Sequence:

ATGATGTGTG AGGTGATGCC AACCATCAGT GAGGCCGAGA TTCCCGCCGG GGGAAATGGA 
GGCCACGGTT CAGGTTCCCC GTTGCAATCA GATGCTGATT CCCACTTCGA GCAGTTAATG 
GTGTCCATGC TGGAAGAAAG GGATCGCCTC CTAGAAACGC TGCGAGAAAC TCAAGAGACG 
CTTGCCCTCA CCCAGGGCAA GCTGCATGAG GTTGGCCATG AGAGGGATTC CTTGCAGAGG 
CAGCTCAATA CTGCACTTCC ACAGGAATTT GCAGCACTCA CCAAAGAGCT GAATGTATGC 
AGGGAGCAGC TGCTTGAAAG GGAAGAAGAA ATTGCTGAGC TGAAAGCAGA AAGAAATAAT 
ACGAGGCTAT TACTGGAACA TTTGGAGTGT CTTGTCTCCA GGCATGAGCG ATCCCTCAGG 
ATGACTGTTG TGAAGAGGCA AGCTCAGTCT CCAGCAGGAG TTTCTAGTGA AGTAGAAGTT 
CTCAAAGCAC TAAAATCTTT ATTTGAGCAC CACAAAGCCC TTGATGAAAA GGTAAGGGAG 
AGATTACGAG TAGCACTTGA AAGATGTAGC TTATTGGAAG AAGAACTAGG TACTACACAT 
AAAGAGTTAA TGATTCTGAA AGAGCAAAAT AATCAGAAGA AAACACTTCC AGATGGGATG 
CTGGATATAA ATCATGAACA AGAAAATACA CCAACTACAA ATGGGAAGAG ATCCTCTGAT 
GGTTCATTAT GCCATGATGA AAACCTTGCT AAAGTGATTG AACTCCAAGA CATCATAGAT 
AAGCAAAACA AAGAGCAGAC ACAAATGAAA GAACGCCTCA CTGCTTTGTC CAGCAGAGTA 
ACAGAGCTGG AAGAAGATCT AGACACAGCA AGGAAAGACT TAATAAAATC TGAAGAAATG 
AACACAAAGT TACAGAGGGA TGTACGAGAG ACTCTGGCCC AGAAGGAGGA CATGGAGGAG 
AGAATCACCA CTCTGGAGAA ACGCTACCTC GCTGCACAAC GTGAAGCTAC ATCCGTGCAT 
GACCTCAATG ATAAACTTGA AAATGAAATT GCCACTAAAG ACTCCATGCA CCGACAGAGT 
GAAGATAAGA ACAGGCAACT GCAAGAGCGA CTGGAACTGG CTGAGCAAAA GCTGCAGCAG 
ACCCTGAGAA AAGCTGAGAC TTTGCCAGAG GTGGAGGCTG AGCTGGCACA GAGAGTTGCA 
GCACTTACAA AGGCTGAGGA GAGACATGGC AATATCGAGG AGCGACTGAG ACAGATGGAG 
GCACAGCTTG AAGAGAAGAA TCAAGAACTG CAAAGGGCTA GACAGAGAGA GAAGATGAAT 
GAAGAGCATA ATAAACGTTT GTCAGAAACT GTTGATAAGC TCCTATCTGA ATCCAATGAA 
AGGCTCCAAC TTCATCTCAA GGAGAGGATG GCTGCCCTGG AAGATAAGAA TTCACTTCTT 
CGAGAAATTG AAAATGCAAA GAAACAAATA GAAGAACTTC AGCATGAAAA GGATCAACTT 
GTATTAAATG TGGATGCATT AAGGGCTGAA AATGACCAAG TGAGGCTCAG AGCCACATCA 
CTTCATCATA GCAGACCAGA TTTCAGATTC CCTGTGCCAC CCTCCTCTGT GGGGGACACT 
CACACAGACT CCTACAGCAC CTCCTCTGTG CTGAGGCGGC CCCAGAAGGG GCGTCTGGCT 
GCTCTCAGGG ACGAGCCTTC AAAGGTTCAG ACCCTGAATG AGCAGGATTG GGAGCGGGCC 
CAGCAGGCCA GTGTTTTGGC AAATGTGGCA CAAGCATTTG AGAGTGATGT GGATGTGTCT 
GATGTGGAGG ATGACAGGGA GACCATTTTC AGCTCAGTTG ATCTGCTGTC ACCAAGTGGT 
CAGGCTGATG CTCAGACTTT GGCCATAATG CTGCAGGAAC AGTTGGATGC AATCAACAAA 
GAGATCAGAC TGATCCAGGA GGAGAAGGAG AACACGGAGC AGCGCGCGGA GGAGATTGAG 
AGCCGTGTGG GCAGCGGCAG CCTGGAGGCG CACGCCCGGT TCCGCTCGCT GGGCTCCATC 
CCCCCCGCCT ACGGTGGGGC CCTGGCCGGC TCCTCCCCGC CCGGCAGCGG CCGCTCCACA 
CCCCGGCGCA TCCCACACAG CCCGGCCAGG GAAGTGGACA GGCTGGGCAT CATGACATTG 
TCTCCAGCTT CCAGGGAAGA GGTACGAGAT GACAAAACCA CCATAAAATG TGAGACATCA 
CCACCTTCAT CACCTCGGTC TTTGCGTCTG GACAGAGTCC AGAAAGGAGC TCTGCACACA 
GTGAGCCACG AAGATATCAG GGACATTAGG AATTCAACAG GTTCACAAGA TGGGCAAACA 
AGTAATCCTA GTAGCAGTAA CAGCAGCCAA GATTCCCTTC ACAAAGCCCC CAAAAAAAAG 
GGAATCAAAT CCTCTATTGG CCGCCTGTTT GGCAAGAAGG AAAAGGGACG ACCTGGACAA 
GGAAGCAAGG AATCTCTAGG ACAAGGTGGC ATGGGAGAAG CAGATGGTTC CTCTCAGGAT 
GCATTAGGTC TCAGCAAACT TGGAGGTCAG GCAGAGAAAA ACAGGAAAAT GCAGAAGAAG 
CACGAGTTGC TGGAGGAAGC CAGAAGACAG GGTTTGCCTT TTGCACAGTG GGATGGGCCC 
ACTGTGGTGG TGTGGCTGGA GCTGTGGGTG GGGATGCCAG CCTGGTACGT GGCTGCCTGC 
CGAGCCAATG TGAAAAGCGG TGCTATCATG TCAGCCCTGT CTGACACAGA AATCCAGCGG 
GAAATCGGCA TCAGCAATCC CCTGCACAGG CTCAAGCTCC GCCTGGCCAT CCAGGAAATC 
ATGTCCCTAA CCAGCCCCTC TGCCCCTCCC ACCTCAAGGA CGACCACGGG AAATGTCTGG 
GTAACACATG AAGAGATGGA AAATCTTACA GCCACACAAC AAACGACATT AGCCTATGGT 
GACATGAACC ATGAGTGGAT TGGCAATGAG TGGCTGCCAA GCCTGGGGCT CCCTCAGTAC 
CGCAGCTACT TCATGGAATG TCTGGTGGAT GCTCGGATGC TGGATCACTT AACTAAAAAA 
GACCTGCGTG GGCAGCTGAA AATGGTGGAC AGCTTTCACA GAAACAGTTT TCAGTGTGGA 
ATTATGTGCC TCCGAAGACT GAATTACGAT CGAAAAGAGC TAGAAAGAAA AAGAGAAGAA 
AGCCAGAATG AAATTAAGGA TGTCCTTGTG TGGAGCAATG AGAGGATGAT CCACTGGGTC 
ATGTCCATTG GCCTTAAGGA ATATGCGAAC AACCTGCTCG AGAGCGGAGT TCACGGTGCA 
CTTGTGGCCT TAGATGAATC CTTTGATTAC AATGCATTAG CTCTCCTGCT GCAAATCCCC 
ACTCAAAACA CACAGGCTCG TGCTGTTCTG GAGAGGGAAT TCAATAACCT TCTTGCTATG 
GGCACTGACA GAAGACTTGA TGAAGATGAT GATAAGAGCT TTAGGAGAGC ACCTTCGTGG 
AGAAAGAAGT TTAGACCAAA GGACATAAGA GGTTTAGCTA CTGGATCAGC AGAGACTCTT 
CCTGCAAATT TCAGAGTTAC AACCTCAATG TCTTCACCCT CTATGCAGCC AAAGAAGATG 
CAGATTGATG GCAGTGTATC AGGAACACAA AGATTGGATT CTGCTACAGT AAGGACCTAC 
TCCTGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PPFIA1 ( XM_015631379.2 ) cDNA ORF clone, Parus major(Great Tit) -> XP_015486865.1 Parus major PTPRF interacting protein alpha 1 (PPFIA1), transcript variant X11, mRNA.

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CloneID	OPa179678
Clone ID Related Accession (Same CDS sequence)	XM_015631379.2
Accession Version	XM_015631379.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3606bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-11-13
Organism	Parus major(Great Tit)
Product	liprin-alpha-1 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_031774.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Nov 4, 2016 this sequence version replaced XM_015631379.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Parus major Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTGTG AGGTGATGCC AACCATCAGT GAGGCCGAGA TTCCCGCCGG GGGAAATGGA 
GGCCACGGTT CAGGTTCCCC GTTGCAATCA GATGCTGATT CCCACTTCGA GCAGTTAATG 
GTGTCCATGC TGGAAGAAAG GGATCGCCTC CTAGAAACGC TGCGAGAAAC TCAAGAGACG 
CTTGCCCTCA CCCAGGGCAA GCTGCATGAG GTTGGCCATG AGAGGGATTC CTTGCAGAGG 
CAGCTCAATA CTGCACTTCC ACAGGAATTT GCAGCACTCA CCAAAGAGCT GAATGTATGC 
AGGGAGCAGC TGCTTGAAAG GGAAGAAGAA ATTGCTGAGC TGAAAGCAGA AAGAAATAAT 
ACGAGGCTAT TACTGGAACA TTTGGAGTGT CTTGTCTCCA GGCATGAGCG ATCCCTCAGG 
ATGACTGTTG TGAAGAGGCA AGCTCAGTCT CCAGCAGGAG TTTCTAGTGA AGTAGAAGTT 
CTCAAAGCAC TAAAATCTTT ATTTGAGCAC CACAAAGCCC TTGATGAAAA GGTAAGGGAG 
AGATTACGAG TAGCACTTGA AAGATGTAGC TTATTGGAAG AAGAACTAGG TACTACACAT 
AAAGAGTTAA TGATTCTGAA AGAGCAAAAT AATCAGAAGA AAACACTTCC AGATGGGATG 
CTGGATATAA ATCATGAACA AGAAAATACA CCAACTACAA ATGGGAAGAG ATCCTCTGAT 
GGTTCATTAT GCCATGATGA AAACCTTGCT AAAGTGATTG AACTCCAAGA CATCATAGAT 
AAGCAAAACA AAGAGCAGAC ACAAATGAAA GAACGCCTCA CTGCTTTGTC CAGCAGAGTA 
ACAGAGCTGG AAGAAGATCT AGACACAGCA AGGAAAGACT TAATAAAATC TGAAGAAATG 
AACACAAAGT TACAGAGGGA TGTACGAGAG ACTCTGGCCC AGAAGGAGGA CATGGAGGAG 
AGAATCACCA CTCTGGAGAA ACGCTACCTC GCTGCACAAC GTGAAGCTAC ATCCGTGCAT 
GACCTCAATG ATAAACTTGA AAATGAAATT GCCACTAAAG ACTCCATGCA CCGACAGAGT 
GAAGATAAGA ACAGGCAACT GCAAGAGCGA CTGGAACTGG CTGAGCAAAA GCTGCAGCAG 
ACCCTGAGAA AAGCTGAGAC TTTGCCAGAG GTGGAGGCTG AGCTGGCACA GAGAGTTGCA 
GCACTTACAA AGGCTGAGGA GAGACATGGC AATATCGAGG AGCGACTGAG ACAGATGGAG 
GCACAGCTTG AAGAGAAGAA TCAAGAACTG CAAAGGGCTA GACAGAGAGA GAAGATGAAT 
GAAGAGCATA ATAAACGTTT GTCAGAAACT GTTGATAAGC TCCTATCTGA ATCCAATGAA 
AGGCTCCAAC TTCATCTCAA GGAGAGGATG GCTGCCCTGG AAGATAAGAA TTCACTTCTT 
CGAGAAATTG AAAATGCAAA GAAACAAATA GAAGAACTTC AGCATGAAAA GGATCAACTT 
GTATTAAATG TGGATGCATT AAGGGCTGAA AATGACCAAG TGAGGCTCAG AGCCACATCA 
CTTCATCATA GCAGACCAGA TTTCAGATTC CCTGTGCCAC CCTCCTCTGT GGGGGACACT 
CACACAGACT CCTACAGCAC CTCCTCTGTG CTGAGGCGGC CCCAGAAGGG GCGTCTGGCT 
GCTCTCAGGG ACGAGCCTTC AAAGGTTCAG ACCCTGAATG AGCAGGATTG GGAGCGGGCC 
CAGCAGGCCA GTGTTTTGGC AAATGTGGCA CAAGCATTTG AGAGTGATGT GGATGTGTCT 
GATGTGGAGG ATGACAGGGA GACCATTTTC AGCTCAGTTG ATCTGCTGTC ACCAAGTGGT 
CAGGCTGATG CTCAGACTTT GGCCATAATG CTGCAGGAAC AGTTGGATGC AATCAACAAA 
GAGATCAGAC TGATCCAGGA GGAGAAGGAG AACACGGAGC AGCGCGCGGA GGAGATTGAG 
AGCCGTGTGG GCAGCGGCAG CCTGGAGGCG CACGCCCGGT TCCGCTCGCT GGGCTCCATC 
CCCCCCGCCT ACGGTGGGGC CCTGGCCGGC TCCTCCCCGC CCGGCAGCGG CCGCTCCACA 
CCCCGGCGCA TCCCACACAG CCCGGCCAGG GAAGTGGACA GGCTGGGCAT CATGACATTG 
TCTCCAGCTT CCAGGGAAGA GGTACGAGAT GACAAAACCA CCATAAAATG TGAGACATCA 
CCACCTTCAT CACCTCGGTC TTTGCGTCTG GACAGAGTCC AGAAAGGAGC TCTGCACACA 
GTGAGCCACG AAGATATCAG GGACATTAGG AATTCAACAG GTTCACAAGA TGGGCAAACA 
AGTAATCCTA GTAGCAGTAA CAGCAGCCAA GATTCCCTTC ACAAAGCCCC CAAAAAAAAG 
GGAATCAAAT CCTCTATTGG CCGCCTGTTT GGCAAGAAGG AAAAGGGACG ACCTGGACAA 
GGAAGCAAGG AATCTCTAGG ACAAGGTGGC ATGGGAGAAG CAGATGGTTC CTCTCAGGAT 
GCATTAGGTC TCAGCAAACT TGGAGGTCAG GCAGAGAAAA ACAGGAAAAT GCAGAAGAAG 
CACGAGTTGC TGGAGGAAGC CAGAAGACAG GGTTTGCCTT TTGCACAGTG GGATGGGCCC 
ACTGTGGTGG TGTGGCTGGA GCTGTGGGTG GGGATGCCAG CCTGGTACGT GGCTGCCTGC 
CGAGCCAATG TGAAAAGCGG TGCTATCATG TCAGCCCTGT CTGACACAGA AATCCAGCGG 
GAAATCGGCA TCAGCAATCC CCTGCACAGG CTCAAGCTCC GCCTGGCCAT CCAGGAAATC 
ATGTCCCTAA CCAGCCCCTC TGCCCCTCCC ACCTCAAGGA CGACATTAGC CTATGGTGAC 
ATGAACCATG AGTGGATTGG CAATGAGTGG CTGCCAAGCC TGGGGCTCCC TCAGTACCGC 
AGCTACTTCA TGGAATGTCT GGTGGATGCT CGGATGCTGG ATCACTTAAC TAAAAAAGAC 
CTGCGTGGGC AGCTGAAAAT GGTGGACAGC TTTCACAGAA ACAGTTTTCA GTGTGGAATT 
ATGTGCCTCC GAAGACTGAA TTACGATCGA AAAGAGCTAG AAAGAAAAAG AGAAGAAAGC 
CAGAATGAAA TTAAGGATGT CCTTGTGTGG AGCAATGAGA GGATGATCCA CTGGGTCATG 
TCCATTGGCC TTAAGGAATA TGCGAACAAC CTGCTCGAGA GCGGAGTTCA CGGTGCACTT 
GTGGCCTTAG ATGAATCCTT TGATTACAAT GCATTAGCTC TCCTGCTGCA AATCCCCACT 
CAAAACACAC AGGCTCGTGC TGTTCTGGAG AGGGAATTCA ATAACCTTCT TGCTATGGGC 
ACTGACAGAA GACTTGATGA AGATGATGAT AAGAGCTTTA GGAGAGCACC TTCGTGGAGA 
AAGAAGTTTA GACCAAAGGA CATAAGAGGT TTAGCTACTG GATCAGCAGA GACTCTTCCT 
GCAAATTTCA GAGTTACAAC CTCAATGTCT TCACCCTCTA TGCAGCCAAA GAAGATGCAG 
ATTGATGGCA GTGTATCAGG AACACAAAGA TTGGATTCTG CTACAGTAAG GACCTACTCC 
TGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015486865.1
CDS	912..4517
Translation	MMCEVMPTISEAEIPAGGNGGHGSGSPLQSDADSHFEQLMVSMLEERDRLLETLRETQETLALTQGKLHEVGHERDSLQRQLNTALPQEFAALTKELNVCREQLLEREEEIAELKAERNNTRLLLEHLECLVSRHERSLRMTVVKRQAQSPAGVSSEVEVLKALKSLFEHHKALDEKVRERLRVALERCSLLEEELGTTHKELMILKEQNNQKKTLPDGMLDINHEQENTPTTNGKRSSDGSLCHDENLAKVIELQDIIDKQNKEQTQMKERLTALSSRVTELEEDLDTARKDLIKSEEMNTKLQRDVRETLAQKEDMEERITTLEKRYLAAQREATSVHDLNDKLENEIATKDSMHRQSEDKNRQLQERLELAEQKLQQTLRKAETLPEVEAELAQRVAALTKAEERHGNIEERLRQMEAQLEEKNQELQRARQREKMNEEHNKRLSETVDKLLSESNERLQLHLKERMAALEDKNSLLREIENAKKQIEELQHEKDQLVLNVDALRAENDQVRLRATSLHHSRPDFRFPVPPSSVGDTHTDSYSTSSVLRRPQKGRLAALRDEPSKVQTLNEQDWERAQQASVLANVAQAFESDVDVSDVEDDRETIFSSVDLLSPSGQADAQTLAIMLQEQLDAINKEIRLIQEEKENTEQRAEEIESRVGSGSLEAHARFRSLGSIPPAYGGALAGSSPPGSGRSTPRRIPHSPAREVDRLGIMTLSPASREEVRDDKTTIKCETSPPSSPRSLRLDRVQKGALHTVSHEDIRDIRNSTGSQDGQTSNPSSSNSSQDSLHKAPKKKGIKSSIGRLFGKKEKGRPGQGSKESLGQGGMGEADGSSQDALGLSKLGGQAEKNRKMQKKHELLEEARRQGLPFAQWDGPTVVVWLELWVGMPAWYVAACRANVKSGAIMSALSDTEIQREIGISNPLHRLKLRLAIQEIMSLTSPSAPPTSRTTLAYGDMNHEWIGNEWLPSLGLPQYRSYFMECLVDARMLDHLTKKDLRGQLKMVDSFHRNSFQCGIMCLRRLNYDRKELERKREESQNEIKDVLVWSNERMIHWVMSIGLKEYANNLLESGVHGALVALDESFDYNALALLLQIPTQNTQARAVLEREFNNLLAMGTDRRLDEDDDKSFRRAPSWRKKFRPKDIRGLATGSAETLPANFRVTTSMSSPSMQPKKMQIDGSVSGTQRLDSATVRTYSC

Target ORF information:

RefSeq Version	XM_015631379.2
Organism	Parus major(Great Tit)
Definition	Parus major PTPRF interacting protein alpha 1 (PPFIA1), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015631379.2

ORF Insert Sequence:

ATGATGTGTG AGGTGATGCC AACCATCAGT GAGGCCGAGA TTCCCGCCGG GGGAAATGGA 
GGCCACGGTT CAGGTTCCCC GTTGCAATCA GATGCTGATT CCCACTTCGA GCAGTTAATG 
GTGTCCATGC TGGAAGAAAG GGATCGCCTC CTAGAAACGC TGCGAGAAAC TCAAGAGACG 
CTTGCCCTCA CCCAGGGCAA GCTGCATGAG GTTGGCCATG AGAGGGATTC CTTGCAGAGG 
CAGCTCAATA CTGCACTTCC ACAGGAATTT GCAGCACTCA CCAAAGAGCT GAATGTATGC 
AGGGAGCAGC TGCTTGAAAG GGAAGAAGAA ATTGCTGAGC TGAAAGCAGA AAGAAATAAT 
ACGAGGCTAT TACTGGAACA TTTGGAGTGT CTTGTCTCCA GGCATGAGCG ATCCCTCAGG 
ATGACTGTTG TGAAGAGGCA AGCTCAGTCT CCAGCAGGAG TTTCTAGTGA AGTAGAAGTT 
CTCAAAGCAC TAAAATCTTT ATTTGAGCAC CACAAAGCCC TTGATGAAAA GGTAAGGGAG 
AGATTACGAG TAGCACTTGA AAGATGTAGC TTATTGGAAG AAGAACTAGG TACTACACAT 
AAAGAGTTAA TGATTCTGAA AGAGCAAAAT AATCAGAAGA AAACACTTCC AGATGGGATG 
CTGGATATAA ATCATGAACA AGAAAATACA CCAACTACAA ATGGGAAGAG ATCCTCTGAT 
GGTTCATTAT GCCATGATGA AAACCTTGCT AAAGTGATTG AACTCCAAGA CATCATAGAT 
AAGCAAAACA AAGAGCAGAC ACAAATGAAA GAACGCCTCA CTGCTTTGTC CAGCAGAGTA 
ACAGAGCTGG AAGAAGATCT AGACACAGCA AGGAAAGACT TAATAAAATC TGAAGAAATG 
AACACAAAGT TACAGAGGGA TGTACGAGAG ACTCTGGCCC AGAAGGAGGA CATGGAGGAG 
AGAATCACCA CTCTGGAGAA ACGCTACCTC GCTGCACAAC GTGAAGCTAC ATCCGTGCAT 
GACCTCAATG ATAAACTTGA AAATGAAATT GCCACTAAAG ACTCCATGCA CCGACAGAGT 
GAAGATAAGA ACAGGCAACT GCAAGAGCGA CTGGAACTGG CTGAGCAAAA GCTGCAGCAG 
ACCCTGAGAA AAGCTGAGAC TTTGCCAGAG GTGGAGGCTG AGCTGGCACA GAGAGTTGCA 
GCACTTACAA AGGCTGAGGA GAGACATGGC AATATCGAGG AGCGACTGAG ACAGATGGAG 
GCACAGCTTG AAGAGAAGAA TCAAGAACTG CAAAGGGCTA GACAGAGAGA GAAGATGAAT 
GAAGAGCATA ATAAACGTTT GTCAGAAACT GTTGATAAGC TCCTATCTGA ATCCAATGAA 
AGGCTCCAAC TTCATCTCAA GGAGAGGATG GCTGCCCTGG AAGATAAGAA TTCACTTCTT 
CGAGAAATTG AAAATGCAAA GAAACAAATA GAAGAACTTC AGCATGAAAA GGATCAACTT 
GTATTAAATG TGGATGCATT AAGGGCTGAA AATGACCAAG TGAGGCTCAG AGCCACATCA 
CTTCATCATA GCAGACCAGA TTTCAGATTC CCTGTGCCAC CCTCCTCTGT GGGGGACACT 
CACACAGACT CCTACAGCAC CTCCTCTGTG CTGAGGCGGC CCCAGAAGGG GCGTCTGGCT 
GCTCTCAGGG ACGAGCCTTC AAAGGTTCAG ACCCTGAATG AGCAGGATTG GGAGCGGGCC 
CAGCAGGCCA GTGTTTTGGC AAATGTGGCA CAAGCATTTG AGAGTGATGT GGATGTGTCT 
GATGTGGAGG ATGACAGGGA GACCATTTTC AGCTCAGTTG ATCTGCTGTC ACCAAGTGGT 
CAGGCTGATG CTCAGACTTT GGCCATAATG CTGCAGGAAC AGTTGGATGC AATCAACAAA 
GAGATCAGAC TGATCCAGGA GGAGAAGGAG AACACGGAGC AGCGCGCGGA GGAGATTGAG 
AGCCGTGTGG GCAGCGGCAG CCTGGAGGCG CACGCCCGGT TCCGCTCGCT GGGCTCCATC 
CCCCCCGCCT ACGGTGGGGC CCTGGCCGGC TCCTCCCCGC CCGGCAGCGG CCGCTCCACA 
CCCCGGCGCA TCCCACACAG CCCGGCCAGG GAAGTGGACA GGCTGGGCAT CATGACATTG 
TCTCCAGCTT CCAGGGAAGA GGTACGAGAT GACAAAACCA CCATAAAATG TGAGACATCA 
CCACCTTCAT CACCTCGGTC TTTGCGTCTG GACAGAGTCC AGAAAGGAGC TCTGCACACA 
GTGAGCCACG AAGATATCAG GGACATTAGG AATTCAACAG GTTCACAAGA TGGGCAAACA 
AGTAATCCTA GTAGCAGTAA CAGCAGCCAA GATTCCCTTC ACAAAGCCCC CAAAAAAAAG 
GGAATCAAAT CCTCTATTGG CCGCCTGTTT GGCAAGAAGG AAAAGGGACG ACCTGGACAA 
GGAAGCAAGG AATCTCTAGG ACAAGGTGGC ATGGGAGAAG CAGATGGTTC CTCTCAGGAT 
GCATTAGGTC TCAGCAAACT TGGAGGTCAG GCAGAGAAAA ACAGGAAAAT GCAGAAGAAG 
CACGAGTTGC TGGAGGAAGC CAGAAGACAG GGTTTGCCTT TTGCACAGTG GGATGGGCCC 
ACTGTGGTGG TGTGGCTGGA GCTGTGGGTG GGGATGCCAG CCTGGTACGT GGCTGCCTGC 
CGAGCCAATG TGAAAAGCGG TGCTATCATG TCAGCCCTGT CTGACACAGA AATCCAGCGG 
GAAATCGGCA TCAGCAATCC CCTGCACAGG CTCAAGCTCC GCCTGGCCAT CCAGGAAATC 
ATGTCCCTAA CCAGCCCCTC TGCCCCTCCC ACCTCAAGGA CGACATTAGC CTATGGTGAC 
ATGAACCATG AGTGGATTGG CAATGAGTGG CTGCCAAGCC TGGGGCTCCC TCAGTACCGC 
AGCTACTTCA TGGAATGTCT GGTGGATGCT CGGATGCTGG ATCACTTAAC TAAAAAAGAC 
CTGCGTGGGC AGCTGAAAAT GGTGGACAGC TTTCACAGAA ACAGTTTTCA GTGTGGAATT 
ATGTGCCTCC GAAGACTGAA TTACGATCGA AAAGAGCTAG AAAGAAAAAG AGAAGAAAGC 
CAGAATGAAA TTAAGGATGT CCTTGTGTGG AGCAATGAGA GGATGATCCA CTGGGTCATG 
TCCATTGGCC TTAAGGAATA TGCGAACAAC CTGCTCGAGA GCGGAGTTCA CGGTGCACTT 
GTGGCCTTAG ATGAATCCTT TGATTACAAT GCATTAGCTC TCCTGCTGCA AATCCCCACT 
CAAAACACAC AGGCTCGTGC TGTTCTGGAG AGGGAATTCA ATAACCTTCT TGCTATGGGC 
ACTGACAGAA GACTTGATGA AGATGATGAT AAGAGCTTTA GGAGAGCACC TTCGTGGAGA 
AAGAAGTTTA GACCAAAGGA CATAAGAGGT TTAGCTACTG GATCAGCAGA GACTCTTCCT 
GCAAATTTCA GAGTTACAAC CTCAATGTCT TCACCCTCTA TGCAGCCAAA GAAGATGCAG 
ATTGATGGCA GTGTATCAGG AACACAAAGA TTGGATTCTG CTACAGTAAG GACCTACTCC 
TGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PPFIA1 ( XM_015631380.2 ) cDNA ORF clone, Parus major(Great Tit) -> XP_015486866.1 Parus major PTPRF interacting protein alpha 1 (PPFIA1), transcript variant X12, mRNA.

Price & Availability↑

CloneID	OPa179679
Clone ID Related Accession (Same CDS sequence)	XM_015631380.2
Accession Version	XM_015631380.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3663bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-11-13
Organism	Parus major(Great Tit)
Product	liprin-alpha-1 isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_031774.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Nov 4, 2016 this sequence version replaced XM_015631380.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Parus major Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTGTG AGGTGATGCC AACCATCAGT GAGGCCGAGA TTCCCGCCGG GGGAAATGGA 
GGCCACGGTT CAGGTTCCCC GTTGCAATCA GATGCTGATT CCCACTTCGA GCAGTTAATG 
GTGTCCATGC TGGAAGAAAG GGATCGCCTC CTAGAAACGC TGCGAGAAAC TCAAGAGACG 
CTTGCCCTCA CCCAGGGCAA GCTGCATGAG GTTGGCCATG AGAGGGATTC CTTGCAGAGG 
CAGCTCAATA CTGCACTTCC ACAGGAATTT GCAGCACTCA CCAAAGAGCT GAATGTATGC 
AGGGAGCAGC TGCTTGAAAG GGAAGAAGAA ATTGCTGAGC TGAAAGCAGA AAGAAATAAT 
ACGAGGCTAT TACTGGAACA TTTGGAGTGT CTTGTCTCCA GGCATGAGCG ATCCCTCAGG 
ATGACTGTTG TGAAGAGGCA AGCTCAGTCT CCAGCAGGAG TTTCTAGTGA AGTAGAAGTT 
CTCAAAGCAC TAAAATCTTT ATTTGAGCAC CACAAAGCCC TTGATGAAAA GGTAAGGGAG 
AGATTACGAG TAGCACTTGA AAGATGTAGC TTATTGGAAG AAGAACTAGG TACTACACAT 
AAAGAGTTAA TGATTCTGAA AGAGCAAAAT AATCAGAAGA AAACACTTCC AGATGGGATG 
CTGGATATAA ATCATGAACA AGAAAATACA CCAACTACAA ATGGGAAGAG ATCCTCTGAT 
GGTTCATTAT GCCATGATGA AAACCTTGCT AAAGTGATTG AACTCCAAGA CATCATAGAT 
AAGCAAAACA AAGAGCAGAC ACAAATGAAA GAACGCCTCA CTGCTTTGTC CAGCAGAGTA 
ACAGAGCTGG AAGAAGATCT AGACACAGCA AGGAAAGACT TAATAAAATC TGAAGAAATG 
AACACAAAGT TACAGAGGGA TGTACGAGAG ACTCTGGCCC AGAAGGAGGA CATGGAGGAG 
AGAATCACCA CTCTGGAGAA ACGCTACCTC GCTGCACAAC GTGAAGCTAC ATCCGTGCAT 
GACCTCAATG ATAAACTTGA AAATGAAATT GCCACTAAAG ACTCCATGCA CCGACAGAGT 
GAAGATAAGA ACAGGCAACT GCAAGAGCGA CTGGAACTGG CTGAGCAAAA GCTGCAGCAG 
ACCCTGAGAA AAGCTGAGAC TTTGCCAGAG GTGGAGGCTG AGCTGGCACA GAGAGTTGCA 
GCACTTACAA AGGCTGAGGA GAGACATGGC AATATCGAGG AGCGACTGAG ACAGATGGAG 
GCACAGCTTG AAGAGAAGAA TCAAGAACTG CAAAGGGCTA GACAGAGAGA GAAGATGAAT 
GAAGAGCATA ATAAACGTTT GTCAGAAACT GTTGATAAGC TCCTATCTGA ATCCAATGAA 
AGGCTCCAAC TTCATCTCAA GGAGAGGATG GCTGCCCTGG AAGATAAGAA TTCACTTCTT 
CGAGAAATTG AAAATGCAAA GAAACAAATA GAAGAACTTC AGCATGAAAA GGATCAACTT 
GTATTAAATG TGGATGCATT AAGGGCTGAA AATGACCAAG TGAGGCTCAG AGCCACATCA 
CTTCATCATA GCAGACCAGA TTTCAGATTC CCTGTGCCAC CCTCCTCTGT GGGGGACACT 
CACACAGACT CCTACAGCAC CTCCTCTGTG CTGAGGCGGC CCCAGAAGGG GCGTCTGGCT 
GCTCTCAGGG ACGAGCCTTC AAAGGTTCAG ACCCTGAATG AGCAGGATTG GGAGCGGGCC 
CAGCAGGCCA GTGTTTTGGC AAATGTGGCA CAAGCATTTG AGAGTGATGT GGATGTGTCT 
GATGTGGAGG ATGACAGGGA GACCATTTTC AGCTCAGTTG ATCTGCTGTC ACCAAGTGGT 
CAGGCTGATG CTCAGACTTT GGCCATAATG CTGCAGGAAC AGTTGGATGC AATCAACAAA 
GAGATCAGAC TGATCCAGGA GGAGAAGGAG AACACGGAGC AGCGCGCGGA GGAGATTGAG 
AGCCGTGTGG GCAGCGGCAG CCTGGAGGCG CACGCCCGGT TCCGCTCGCT GGGCTCCATC 
CCCCCCGCCT ACGGTGGGGC CCTGGCCGGC TCCTCCCCGC CCGGCAGCGG CCGCTCCACA 
CCCCGGCGCA TCCCACACAG CCCGGCCAGG GAAGTGGACA GGCTGGGCAT CATGACATTG 
CCTAGTGATT TAAGGAAACA CCGTAGAAAG TCTCCAGCTT CCAGGGAAGA GGTACGAGAT 
GACAAAACCA CCATAAAATG TGAGACATCA CCACCTTCAT CACCTCGGTC TTTGCGTCTG 
GACAGAGTCC AGAAAGGAGC TCTGCACACA GTGAGCCACG AAGATATCAG GGACATTAGG 
AATTCAACAG GTTCACAAGA TGGGCAAACA AGTAATCCTA GTAGCAGTAA CAGCAGCCAA 
GATTCCCTTC ACAAAGCCCC CAAAAAAAAG GGAATCAAAT CCTCTATTGG CCGCCTGTTT 
GGCAAGAAGG AAAAGGGACG ACCTGGACAA GGAAGCAAGG AATCTCTAGG ACAAGGTGGC 
ATGGGAGAAG CAGATGGTTC CTCTCAGGAT GCATTAGGTC TCAGCAAACT TGGAGGTCAG 
GCAGAGAAAA ACAGGAAAAT GCAGAAGAAG CACGAGTTGC TGGAGGAAGC CAGAAGACAG 
GGTTTGCCTT TTGCACAGTG GGATGGGCCC ACTGTGGTGG TGTGGCTGGA GCTGTGGGTG 
GGGATGCCAG CCTGGTACGT GGCTGCCTGC CGAGCCAATG TGAAAAGCGG TGCTATCATG 
TCAGCCCTGT CTGACACAGA AATCCAGCGG GAAATCGGCA TCAGCAATCC CCTGCACAGG 
CTCAAGCTCC GCCTGGCCAT CCAGGAAATC ATGTCCCTAA CCAGCCCCTC TGCCCCTCCC 
ACCTCAAGGA CGGAAGATGA GGAGGGAAGC TGGGCTCAGA CATTAGCCTA TGGTGACATG 
AACCATGAGT GGATTGGCAA TGAGTGGCTG CCAAGCCTGG GGCTCCCTCA GTACCGCAGC 
TACTTCATGG AATGTCTGGT GGATGCTCGG ATGCTGGATC ACTTAACTAA AAAAGACCTG 
CGTGGGCAGC TGAAAATGGT GGACAGCTTT CACAGAAACA GTTTTCAGTG TGGAATTATG 
TGCCTCCGAA GACTGAATTA CGATCGAAAA GAGCTAGAAA GAAAAAGAGA AGAAAGCCAG 
AATGAAATTA AGGATGTCCT TGTGTGGAGC AATGAGAGGA TGATCCACTG GGTCATGTCC 
ATTGGCCTTA AGGAATATGC GAACAACCTG CTCGAGAGCG GAGTTCACGG TGCACTTGTG 
GCCTTAGATG AATCCTTTGA TTACAATGCA TTAGCTCTCC TGCTGCAAAT CCCCACTCAA 
AACACACAGG CTCGTGCTGT TCTGGAGAGG GAATTCAATA ACCTTCTTGC TATGGGCACT 
GACAGAAGAC TTGATGAAGA TGATGATAAG AGCTTTAGGA GAGCACCTTC GTGGAGAAAG 
AAGTTTAGAC CAAAGGACAT AAGAGGTTTA GCTACTGGAT CAGCAGAGAC TCTTCCTGCA 
AATTTCAGAG TTACAACCTC AATGTCTTCA CCCTCTATGC AGCCAAAGAA GATGCAGATT 
GATGGCAGTG TATCAGGAAC ACAAAGATTG GATTCTGCTA CAGTAAGGAC CTACTCCTGT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015486866.1
CDS	913..4575
Translation	MMCEVMPTISEAEIPAGGNGGHGSGSPLQSDADSHFEQLMVSMLEERDRLLETLRETQETLALTQGKLHEVGHERDSLQRQLNTALPQEFAALTKELNVCREQLLEREEEIAELKAERNNTRLLLEHLECLVSRHERSLRMTVVKRQAQSPAGVSSEVEVLKALKSLFEHHKALDEKVRERLRVALERCSLLEEELGTTHKELMILKEQNNQKKTLPDGMLDINHEQENTPTTNGKRSSDGSLCHDENLAKVIELQDIIDKQNKEQTQMKERLTALSSRVTELEEDLDTARKDLIKSEEMNTKLQRDVRETLAQKEDMEERITTLEKRYLAAQREATSVHDLNDKLENEIATKDSMHRQSEDKNRQLQERLELAEQKLQQTLRKAETLPEVEAELAQRVAALTKAEERHGNIEERLRQMEAQLEEKNQELQRARQREKMNEEHNKRLSETVDKLLSESNERLQLHLKERMAALEDKNSLLREIENAKKQIEELQHEKDQLVLNVDALRAENDQVRLRATSLHHSRPDFRFPVPPSSVGDTHTDSYSTSSVLRRPQKGRLAALRDEPSKVQTLNEQDWERAQQASVLANVAQAFESDVDVSDVEDDRETIFSSVDLLSPSGQADAQTLAIMLQEQLDAINKEIRLIQEEKENTEQRAEEIESRVGSGSLEAHARFRSLGSIPPAYGGALAGSSPPGSGRSTPRRIPHSPAREVDRLGIMTLPSDLRKHRRKSPASREEVRDDKTTIKCETSPPSSPRSLRLDRVQKGALHTVSHEDIRDIRNSTGSQDGQTSNPSSSNSSQDSLHKAPKKKGIKSSIGRLFGKKEKGRPGQGSKESLGQGGMGEADGSSQDALGLSKLGGQAEKNRKMQKKHELLEEARRQGLPFAQWDGPTVVVWLELWVGMPAWYVAACRANVKSGAIMSALSDTEIQREIGISNPLHRLKLRLAIQEIMSLTSPSAPPTSRTEDEEGSWAQTLAYGDMNHEWIGNEWLPSLGLPQYRSYFMECLVDARMLDHLTKKDLRGQLKMVDSFHRNSFQCGIMCLRRLNYDRKELERKREESQNEIKDVLVWSNERMIHWVMSIGLKEYANNLLESGVHGALVALDESFDYNALALLLQIPTQNTQARAVLEREFNNLLAMGTDRRLDEDDDKSFRRAPSWRKKFRPKDIRGLATGSAETLPANFRVTTSMSSPSMQPKKMQIDGSVSGTQRLDSATVRTYSC

Target ORF information:

RefSeq Version	XM_015631380.2
Organism	Parus major(Great Tit)
Definition	Parus major PTPRF interacting protein alpha 1 (PPFIA1), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015631380.2

ORF Insert Sequence:

ATGATGTGTG AGGTGATGCC AACCATCAGT GAGGCCGAGA TTCCCGCCGG GGGAAATGGA 
GGCCACGGTT CAGGTTCCCC GTTGCAATCA GATGCTGATT CCCACTTCGA GCAGTTAATG 
GTGTCCATGC TGGAAGAAAG GGATCGCCTC CTAGAAACGC TGCGAGAAAC TCAAGAGACG 
CTTGCCCTCA CCCAGGGCAA GCTGCATGAG GTTGGCCATG AGAGGGATTC CTTGCAGAGG 
CAGCTCAATA CTGCACTTCC ACAGGAATTT GCAGCACTCA CCAAAGAGCT GAATGTATGC 
AGGGAGCAGC TGCTTGAAAG GGAAGAAGAA ATTGCTGAGC TGAAAGCAGA AAGAAATAAT 
ACGAGGCTAT TACTGGAACA TTTGGAGTGT CTTGTCTCCA GGCATGAGCG ATCCCTCAGG 
ATGACTGTTG TGAAGAGGCA AGCTCAGTCT CCAGCAGGAG TTTCTAGTGA AGTAGAAGTT 
CTCAAAGCAC TAAAATCTTT ATTTGAGCAC CACAAAGCCC TTGATGAAAA GGTAAGGGAG 
AGATTACGAG TAGCACTTGA AAGATGTAGC TTATTGGAAG AAGAACTAGG TACTACACAT 
AAAGAGTTAA TGATTCTGAA AGAGCAAAAT AATCAGAAGA AAACACTTCC AGATGGGATG 
CTGGATATAA ATCATGAACA AGAAAATACA CCAACTACAA ATGGGAAGAG ATCCTCTGAT 
GGTTCATTAT GCCATGATGA AAACCTTGCT AAAGTGATTG AACTCCAAGA CATCATAGAT 
AAGCAAAACA AAGAGCAGAC ACAAATGAAA GAACGCCTCA CTGCTTTGTC CAGCAGAGTA 
ACAGAGCTGG AAGAAGATCT AGACACAGCA AGGAAAGACT TAATAAAATC TGAAGAAATG 
AACACAAAGT TACAGAGGGA TGTACGAGAG ACTCTGGCCC AGAAGGAGGA CATGGAGGAG 
AGAATCACCA CTCTGGAGAA ACGCTACCTC GCTGCACAAC GTGAAGCTAC ATCCGTGCAT 
GACCTCAATG ATAAACTTGA AAATGAAATT GCCACTAAAG ACTCCATGCA CCGACAGAGT 
GAAGATAAGA ACAGGCAACT GCAAGAGCGA CTGGAACTGG CTGAGCAAAA GCTGCAGCAG 
ACCCTGAGAA AAGCTGAGAC TTTGCCAGAG GTGGAGGCTG AGCTGGCACA GAGAGTTGCA 
GCACTTACAA AGGCTGAGGA GAGACATGGC AATATCGAGG AGCGACTGAG ACAGATGGAG 
GCACAGCTTG AAGAGAAGAA TCAAGAACTG CAAAGGGCTA GACAGAGAGA GAAGATGAAT 
GAAGAGCATA ATAAACGTTT GTCAGAAACT GTTGATAAGC TCCTATCTGA ATCCAATGAA 
AGGCTCCAAC TTCATCTCAA GGAGAGGATG GCTGCCCTGG AAGATAAGAA TTCACTTCTT 
CGAGAAATTG AAAATGCAAA GAAACAAATA GAAGAACTTC AGCATGAAAA GGATCAACTT 
GTATTAAATG TGGATGCATT AAGGGCTGAA AATGACCAAG TGAGGCTCAG AGCCACATCA 
CTTCATCATA GCAGACCAGA TTTCAGATTC CCTGTGCCAC CCTCCTCTGT GGGGGACACT 
CACACAGACT CCTACAGCAC CTCCTCTGTG CTGAGGCGGC CCCAGAAGGG GCGTCTGGCT 
GCTCTCAGGG ACGAGCCTTC AAAGGTTCAG ACCCTGAATG AGCAGGATTG GGAGCGGGCC 
CAGCAGGCCA GTGTTTTGGC AAATGTGGCA CAAGCATTTG AGAGTGATGT GGATGTGTCT 
GATGTGGAGG ATGACAGGGA GACCATTTTC AGCTCAGTTG ATCTGCTGTC ACCAAGTGGT 
CAGGCTGATG CTCAGACTTT GGCCATAATG CTGCAGGAAC AGTTGGATGC AATCAACAAA 
GAGATCAGAC TGATCCAGGA GGAGAAGGAG AACACGGAGC AGCGCGCGGA GGAGATTGAG 
AGCCGTGTGG GCAGCGGCAG CCTGGAGGCG CACGCCCGGT TCCGCTCGCT GGGCTCCATC 
CCCCCCGCCT ACGGTGGGGC CCTGGCCGGC TCCTCCCCGC CCGGCAGCGG CCGCTCCACA 
CCCCGGCGCA TCCCACACAG CCCGGCCAGG GAAGTGGACA GGCTGGGCAT CATGACATTG 
CCTAGTGATT TAAGGAAACA CCGTAGAAAG TCTCCAGCTT CCAGGGAAGA GGTACGAGAT 
GACAAAACCA CCATAAAATG TGAGACATCA CCACCTTCAT CACCTCGGTC TTTGCGTCTG 
GACAGAGTCC AGAAAGGAGC TCTGCACACA GTGAGCCACG AAGATATCAG GGACATTAGG 
AATTCAACAG GTTCACAAGA TGGGCAAACA AGTAATCCTA GTAGCAGTAA CAGCAGCCAA 
GATTCCCTTC ACAAAGCCCC CAAAAAAAAG GGAATCAAAT CCTCTATTGG CCGCCTGTTT 
GGCAAGAAGG AAAAGGGACG ACCTGGACAA GGAAGCAAGG AATCTCTAGG ACAAGGTGGC 
ATGGGAGAAG CAGATGGTTC CTCTCAGGAT GCATTAGGTC TCAGCAAACT TGGAGGTCAG 
GCAGAGAAAA ACAGGAAAAT GCAGAAGAAG CACGAGTTGC TGGAGGAAGC CAGAAGACAG 
GGTTTGCCTT TTGCACAGTG GGATGGGCCC ACTGTGGTGG TGTGGCTGGA GCTGTGGGTG 
GGGATGCCAG CCTGGTACGT GGCTGCCTGC CGAGCCAATG TGAAAAGCGG TGCTATCATG 
TCAGCCCTGT CTGACACAGA AATCCAGCGG GAAATCGGCA TCAGCAATCC CCTGCACAGG 
CTCAAGCTCC GCCTGGCCAT CCAGGAAATC ATGTCCCTAA CCAGCCCCTC TGCCCCTCCC 
ACCTCAAGGA CGGAAGATGA GGAGGGAAGC TGGGCTCAGA CATTAGCCTA TGGTGACATG 
AACCATGAGT GGATTGGCAA TGAGTGGCTG CCAAGCCTGG GGCTCCCTCA GTACCGCAGC 
TACTTCATGG AATGTCTGGT GGATGCTCGG ATGCTGGATC ACTTAACTAA AAAAGACCTG 
CGTGGGCAGC TGAAAATGGT GGACAGCTTT CACAGAAACA GTTTTCAGTG TGGAATTATG 
TGCCTCCGAA GACTGAATTA CGATCGAAAA GAGCTAGAAA GAAAAAGAGA AGAAAGCCAG 
AATGAAATTA AGGATGTCCT TGTGTGGAGC AATGAGAGGA TGATCCACTG GGTCATGTCC 
ATTGGCCTTA AGGAATATGC GAACAACCTG CTCGAGAGCG GAGTTCACGG TGCACTTGTG 
GCCTTAGATG AATCCTTTGA TTACAATGCA TTAGCTCTCC TGCTGCAAAT CCCCACTCAA 
AACACACAGG CTCGTGCTGT TCTGGAGAGG GAATTCAATA ACCTTCTTGC TATGGGCACT 
GACAGAAGAC TTGATGAAGA TGATGATAAG AGCTTTAGGA GAGCACCTTC GTGGAGAAAG 
AAGTTTAGAC CAAAGGACAT AAGAGGTTTA GCTACTGGAT CAGCAGAGAC TCTTCCTGCA 
AATTTCAGAG TTACAACCTC AATGTCTTCA CCCTCTATGC AGCCAAAGAA GATGCAGATT 
GATGGCAGTG TATCAGGAAC ACAAAGATTG GATTCTGCTA CAGTAAGGAC CTACTCCTGT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PPFIA1 ( XM_015631381.2 ) cDNA ORF clone, Parus major(Great Tit) -> XP_015486867.1 Parus major PTPRF interacting protein alpha 1 (PPFIA1), transcript variant X13, mRNA.

Price & Availability↑

CloneID	OPa179680
Clone ID Related Accession (Same CDS sequence)	XM_015631381.2
Accession Version	XM_015631381.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3699bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-11-13
Organism	Parus major(Great Tit)
Product	liprin-alpha-1 isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_031774.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Nov 4, 2016 this sequence version replaced XM_015631381.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Parus major Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTGTG AGGTGATGCC AACCATCAGT GAGGCCGAGA TTCCCGCCGG GGGAAATGGA 
GGCCACGGTT CAGGTTCCCC GTTGCAATCA GATGCTGATT CCCACTTCGA GCAGTTAATG 
GTGTCCATGC TGGAAGAAAG GGATCGCCTC CTAGAAACGC TGCGAGAAAC TCAAGAGACG 
CTTGCCCTCA CCCAGGGCAA GCTGCATGAG GTTGGCCATG AGAGGGATTC CTTGCAGAGG 
CAGCTCAATA CTGCACTTCC ACAGGAATTT GCAGCACTCA CCAAAGAGCT GAATGTATGC 
AGGGAGCAGC TGCTTGAAAG GGAAGAAGAA ATTGCTGAGC TGAAAGCAGA AAGAAATAAT 
ACGAGGCTAT TACTGGAACA TTTGGAGTGT CTTGTCTCCA GGCATGAGCG ATCCCTCAGG 
ATGACTGTTG TGAAGAGGCA AGCTCAGTCT CCAGCAGGAG TTTCTAGTGA AGTAGAAGTT 
CTCAAAGCAC TAAAATCTTT ATTTGAGCAC CACAAAGCCC TTGATGAAAA GGTAAGGGAG 
AGATTACGAG TAGCACTTGA AAGATGTAGC TTATTGGAAG AAGAACTAGG TACTACACAT 
AAAGAGTTAA TGATTCTGAA AGAGCAAAAT AATCAGAAGA AAACACTTCC AGATGGGATG 
CTGGATATAA ATCATGAACA AGAAAATACA CCAACTACAA ATGGGAAGAG ATCCTCTGAT 
GGTTCATTAT GCCATGATGA AAACCTTGCT AAAGTGATTG AACTCCAAGA CATCATAGAT 
AAGCAAAACA AAGAGCAGAC ACAAATGAAA GAACGCCTCA CTGCTTTGTC CAGCAGAGTA 
ACAGAGCTGG AAGAAGATCT AGACACAGCA AGGAAAGACT TAATAAAATC TGAAGAAATG 
AACACAAAGT TACAGAGGGA TGTACGAGAG ACTCTGGCCC AGAAGGAGGA CATGGAGGAG 
AGAATCACCA CTCTGGAGAA ACGCTACCTC GCTGCACAAC GTGAAGCTAC ATCCGTGCAT 
GACCTCAATG ATAAACTTGA AAATGAAATT GCCACTAAAG ACTCCATGCA CCGACAGAGT 
GAAGATAAGA ACAGGCAACT GCAAGAGCGA CTGGAACTGG CTGAGCAAAA GCTGCAGCAG 
ACCCTGAGAA AAGCTGAGAC TTTGCCAGAG GTGGAGGCTG AGCTGGCACA GAGAGTTGCA 
GCACTTACAA AGGCTGAGGA GAGACATGGC AATATCGAGG AGCGACTGAG ACAGATGGAG 
GCACAGCTTG AAGAGAAGAA TCAAGAACTG CAAAGGGCTA GACAGAGAGA GAAGATGAAT 
GAAGAGCATA ATAAACGTTT GTCAGAAACT GTTGATAAGC TCCTATCTGA ATCCAATGAA 
AGGCTCCAAC TTCATCTCAA GGAGAGGATG GCTGCCCTGG AAGATAAGAA TTCACTTCTT 
CGAGAAATTG AAAATGCAAA GAAACAAATA GAAGAACTTC AGCATGAAAA GGATCAACTT 
GTATTAAATG TGGATGCATT AAGGGCTGAA AATGACCAAG TGAGGCTCAG AGCCACATCA 
CTTCATCATA GCAGACCAGA TTTCAGATTC CCTGTGCCAC CCTCCTCTGT GGGGGACACT 
CACACAGACT CCTACAGCAC CTCCTCTGTG CTGAGGCGGC CCCAGAAGGG GCGTCTGGCT 
GCTCTCAGGG ACGAGCCTTC AAAGGTTCAG ACCCTGAATG AGCAGGATTG GGAGCGGGCC 
CAGCAGGCCA GTGTTTTGGC AAATGTGGCA CAAGCATTTG AGAGTGATGT GGATGTGTCT 
GATGTGGAGG ATGACAGGGA GACCATTTTC AGCTCAGTTG ATCTGCTGTC ACCAAGTGGT 
CAGGCTGATG CTCAGACTTT GGCCATAATG CTGCAGGAAC AGTTGGATGC AATCAACAAA 
GAGATCAGAC TGATCCAGGA GGAGAAGGAG AACACGGAGC AGCGCGCGGA GGAGATTGAG 
AGCCGTGTGG GCAGCGGCAG CCTGGAGGCG CACGCCCGGT TCCGCTCGCT GGGCTCCATC 
CCCCCCGCCT ACGGTGGGGC CCTGGCCGGC TCCTCCCCGC CCGGCAGCGG CCGCTCCACA 
CCCCGGCGCA TCCCACACAG CCCGGCCAGG GAAGTGGACA GGCTGGGCAT CATGACATTG 
CCTAGTGATT TAAGGAAACA CCGTAGAAAG TCTCCAGCTT CCAGGGAAGA GGTACGAGAT 
GACAAAACCA CCATAAAATG TGAGACATCA CCACCTTCAT CACCTCGGTC TTTGCGTCTG 
GACAGAGTCC AGAAAGGAGC TCTGCACACA GTGAGCCACG AAGATATCAG GGACATTAGG 
AATTCAACAG GTTCACAAGA TGGGCAAACA AGTAATCCTA GTAGCAGTAA CAGCAGCCAA 
GATTCCCTTC ACAAAGCCCC CAAAAAAAAG GGAATCAAAT CCTCTATTGG CCGCCTGTTT 
GGCAAGAAGG AAAAGGGACG ACCTGGACAA GGAAGCAAGG AATCTCTAGG ACAAGGTGGC 
ATGGGAGAAG CAGATGGTTC CTCTCAGGAT GCATTAGGTC TCAGCAAACT TGGAGGTCAG 
GCAGAGAAAA ACAGGAAAAT GCAGAAGAAG CACGAGTTGC TGGAGGAAGC CAGAAGACAG 
GGTTTGCCTT TTGCACAGTG GGATGGGCCC ACTGTGGTGG TGTGGCTGGA GCTGTGGGTG 
GGGATGCCAG CCTGGTACGT GGCTGCCTGC CGAGCCAATG TGAAAAGCGG TGCTATCATG 
TCAGCCCTGT CTGACACAGA AATCCAGCGG GAAATCGGCA TCAGCAATCC CCTGCACAGG 
CTCAAGCTCC GCCTGGCCAT CCAGGAAATC ATGTCCCTAA CCAGCCCCTC TGCCCCTCCC 
ACCTCAAGGA CGACCACGGG AAATGTCTGG GTAACACATG AAGAGATGGA AAATCTTACA 
GCCACACAAC AAACGACATT AGCCTATGGT GACATGAACC ATGAGTGGAT TGGCAATGAG 
TGGCTGCCAA GCCTGGGGCT CCCTCAGTAC CGCAGCTACT TCATGGAATG TCTGGTGGAT 
GCTCGGATGC TGGATCACTT AACTAAAAAA GACCTGCGTG GGCAGCTGAA AATGGTGGAC 
AGCTTTCACA GAAACAGTTT TCAGTGTGGA ATTATGTGCC TCCGAAGACT GAATTACGAT 
CGAAAAGAGC TAGAAAGAAA AAGAGAAGAA AGCCAGAATG AAATTAAGGA TGTCCTTGTG 
TGGAGCAATG AGAGGATGAT CCACTGGGTC ATGTCCATTG GCCTTAAGGA ATATGCGAAC 
AACCTGCTCG AGAGCGGAGT TCACGGTGCA CTTGTGGCCT TAGATGAATC CTTTGATTAC 
AATGCATTAG CTCTCCTGCT GCAAATCCCC ACTCAAAACA CACAGGCTCG TGCTGTTCTG 
GAGAGGGAAT TCAATAACCT TCTTGCTATG GGCACTGACA GAAGACTTGA TGAAGATGAT 
GATAAGAGCT TTAGGAGAGC ACCTTCGTGG AGAAAGAAGT TTAGACCAAA GGACATAAGA 
GGTTTAGCTA CTGGATCAGC AGAGACTCTT CCTGCAAATT TCAGAGTTAC AACCTCAATG 
TCTTCACCCT CTATGCAGCC AAAGAAGATG CAGATTGATG GCAGTGTATC AGGAACACAA 
AGATTGGATT CTGCTACAGT AAGGACCTAC TCCTGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015486867.1
CDS	913..4611
Translation	MMCEVMPTISEAEIPAGGNGGHGSGSPLQSDADSHFEQLMVSMLEERDRLLETLRETQETLALTQGKLHEVGHERDSLQRQLNTALPQEFAALTKELNVCREQLLEREEEIAELKAERNNTRLLLEHLECLVSRHERSLRMTVVKRQAQSPAGVSSEVEVLKALKSLFEHHKALDEKVRERLRVALERCSLLEEELGTTHKELMILKEQNNQKKTLPDGMLDINHEQENTPTTNGKRSSDGSLCHDENLAKVIELQDIIDKQNKEQTQMKERLTALSSRVTELEEDLDTARKDLIKSEEMNTKLQRDVRETLAQKEDMEERITTLEKRYLAAQREATSVHDLNDKLENEIATKDSMHRQSEDKNRQLQERLELAEQKLQQTLRKAETLPEVEAELAQRVAALTKAEERHGNIEERLRQMEAQLEEKNQELQRARQREKMNEEHNKRLSETVDKLLSESNERLQLHLKERMAALEDKNSLLREIENAKKQIEELQHEKDQLVLNVDALRAENDQVRLRATSLHHSRPDFRFPVPPSSVGDTHTDSYSTSSVLRRPQKGRLAALRDEPSKVQTLNEQDWERAQQASVLANVAQAFESDVDVSDVEDDRETIFSSVDLLSPSGQADAQTLAIMLQEQLDAINKEIRLIQEEKENTEQRAEEIESRVGSGSLEAHARFRSLGSIPPAYGGALAGSSPPGSGRSTPRRIPHSPAREVDRLGIMTLPSDLRKHRRKSPASREEVRDDKTTIKCETSPPSSPRSLRLDRVQKGALHTVSHEDIRDIRNSTGSQDGQTSNPSSSNSSQDSLHKAPKKKGIKSSIGRLFGKKEKGRPGQGSKESLGQGGMGEADGSSQDALGLSKLGGQAEKNRKMQKKHELLEEARRQGLPFAQWDGPTVVVWLELWVGMPAWYVAACRANVKSGAIMSALSDTEIQREIGISNPLHRLKLRLAIQEIMSLTSPSAPPTSRTTTGNVWVTHEEMENLTATQQTTLAYGDMNHEWIGNEWLPSLGLPQYRSYFMECLVDARMLDHLTKKDLRGQLKMVDSFHRNSFQCGIMCLRRLNYDRKELERKREESQNEIKDVLVWSNERMIHWVMSIGLKEYANNLLESGVHGALVALDESFDYNALALLLQIPTQNTQARAVLEREFNNLLAMGTDRRLDEDDDKSFRRAPSWRKKFRPKDIRGLATGSAETLPANFRVTTSMSSPSMQPKKMQIDGSVSGTQRLDSATVRTYSC

Target ORF information:

RefSeq Version	XM_015631381.2
Organism	Parus major(Great Tit)
Definition	Parus major PTPRF interacting protein alpha 1 (PPFIA1), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015631381.2

ORF Insert Sequence:

ATGATGTGTG AGGTGATGCC AACCATCAGT GAGGCCGAGA TTCCCGCCGG GGGAAATGGA 
GGCCACGGTT CAGGTTCCCC GTTGCAATCA GATGCTGATT CCCACTTCGA GCAGTTAATG 
GTGTCCATGC TGGAAGAAAG GGATCGCCTC CTAGAAACGC TGCGAGAAAC TCAAGAGACG 
CTTGCCCTCA CCCAGGGCAA GCTGCATGAG GTTGGCCATG AGAGGGATTC CTTGCAGAGG 
CAGCTCAATA CTGCACTTCC ACAGGAATTT GCAGCACTCA CCAAAGAGCT GAATGTATGC 
AGGGAGCAGC TGCTTGAAAG GGAAGAAGAA ATTGCTGAGC TGAAAGCAGA AAGAAATAAT 
ACGAGGCTAT TACTGGAACA TTTGGAGTGT CTTGTCTCCA GGCATGAGCG ATCCCTCAGG 
ATGACTGTTG TGAAGAGGCA AGCTCAGTCT CCAGCAGGAG TTTCTAGTGA AGTAGAAGTT 
CTCAAAGCAC TAAAATCTTT ATTTGAGCAC CACAAAGCCC TTGATGAAAA GGTAAGGGAG 
AGATTACGAG TAGCACTTGA AAGATGTAGC TTATTGGAAG AAGAACTAGG TACTACACAT 
AAAGAGTTAA TGATTCTGAA AGAGCAAAAT AATCAGAAGA AAACACTTCC AGATGGGATG 
CTGGATATAA ATCATGAACA AGAAAATACA CCAACTACAA ATGGGAAGAG ATCCTCTGAT 
GGTTCATTAT GCCATGATGA AAACCTTGCT AAAGTGATTG AACTCCAAGA CATCATAGAT 
AAGCAAAACA AAGAGCAGAC ACAAATGAAA GAACGCCTCA CTGCTTTGTC CAGCAGAGTA 
ACAGAGCTGG AAGAAGATCT AGACACAGCA AGGAAAGACT TAATAAAATC TGAAGAAATG 
AACACAAAGT TACAGAGGGA TGTACGAGAG ACTCTGGCCC AGAAGGAGGA CATGGAGGAG 
AGAATCACCA CTCTGGAGAA ACGCTACCTC GCTGCACAAC GTGAAGCTAC ATCCGTGCAT 
GACCTCAATG ATAAACTTGA AAATGAAATT GCCACTAAAG ACTCCATGCA CCGACAGAGT 
GAAGATAAGA ACAGGCAACT GCAAGAGCGA CTGGAACTGG CTGAGCAAAA GCTGCAGCAG 
ACCCTGAGAA AAGCTGAGAC TTTGCCAGAG GTGGAGGCTG AGCTGGCACA GAGAGTTGCA 
GCACTTACAA AGGCTGAGGA GAGACATGGC AATATCGAGG AGCGACTGAG ACAGATGGAG 
GCACAGCTTG AAGAGAAGAA TCAAGAACTG CAAAGGGCTA GACAGAGAGA GAAGATGAAT 
GAAGAGCATA ATAAACGTTT GTCAGAAACT GTTGATAAGC TCCTATCTGA ATCCAATGAA 
AGGCTCCAAC TTCATCTCAA GGAGAGGATG GCTGCCCTGG AAGATAAGAA TTCACTTCTT 
CGAGAAATTG AAAATGCAAA GAAACAAATA GAAGAACTTC AGCATGAAAA GGATCAACTT 
GTATTAAATG TGGATGCATT AAGGGCTGAA AATGACCAAG TGAGGCTCAG AGCCACATCA 
CTTCATCATA GCAGACCAGA TTTCAGATTC CCTGTGCCAC CCTCCTCTGT GGGGGACACT 
CACACAGACT CCTACAGCAC CTCCTCTGTG CTGAGGCGGC CCCAGAAGGG GCGTCTGGCT 
GCTCTCAGGG ACGAGCCTTC AAAGGTTCAG ACCCTGAATG AGCAGGATTG GGAGCGGGCC 
CAGCAGGCCA GTGTTTTGGC AAATGTGGCA CAAGCATTTG AGAGTGATGT GGATGTGTCT 
GATGTGGAGG ATGACAGGGA GACCATTTTC AGCTCAGTTG ATCTGCTGTC ACCAAGTGGT 
CAGGCTGATG CTCAGACTTT GGCCATAATG CTGCAGGAAC AGTTGGATGC AATCAACAAA 
GAGATCAGAC TGATCCAGGA GGAGAAGGAG AACACGGAGC AGCGCGCGGA GGAGATTGAG 
AGCCGTGTGG GCAGCGGCAG CCTGGAGGCG CACGCCCGGT TCCGCTCGCT GGGCTCCATC 
CCCCCCGCCT ACGGTGGGGC CCTGGCCGGC TCCTCCCCGC CCGGCAGCGG CCGCTCCACA 
CCCCGGCGCA TCCCACACAG CCCGGCCAGG GAAGTGGACA GGCTGGGCAT CATGACATTG 
CCTAGTGATT TAAGGAAACA CCGTAGAAAG TCTCCAGCTT CCAGGGAAGA GGTACGAGAT 
GACAAAACCA CCATAAAATG TGAGACATCA CCACCTTCAT CACCTCGGTC TTTGCGTCTG 
GACAGAGTCC AGAAAGGAGC TCTGCACACA GTGAGCCACG AAGATATCAG GGACATTAGG 
AATTCAACAG GTTCACAAGA TGGGCAAACA AGTAATCCTA GTAGCAGTAA CAGCAGCCAA 
GATTCCCTTC ACAAAGCCCC CAAAAAAAAG GGAATCAAAT CCTCTATTGG CCGCCTGTTT 
GGCAAGAAGG AAAAGGGACG ACCTGGACAA GGAAGCAAGG AATCTCTAGG ACAAGGTGGC 
ATGGGAGAAG CAGATGGTTC CTCTCAGGAT GCATTAGGTC TCAGCAAACT TGGAGGTCAG 
GCAGAGAAAA ACAGGAAAAT GCAGAAGAAG CACGAGTTGC TGGAGGAAGC CAGAAGACAG 
GGTTTGCCTT TTGCACAGTG GGATGGGCCC ACTGTGGTGG TGTGGCTGGA GCTGTGGGTG 
GGGATGCCAG CCTGGTACGT GGCTGCCTGC CGAGCCAATG TGAAAAGCGG TGCTATCATG 
TCAGCCCTGT CTGACACAGA AATCCAGCGG GAAATCGGCA TCAGCAATCC CCTGCACAGG 
CTCAAGCTCC GCCTGGCCAT CCAGGAAATC ATGTCCCTAA CCAGCCCCTC TGCCCCTCCC 
ACCTCAAGGA CGACCACGGG AAATGTCTGG GTAACACATG AAGAGATGGA AAATCTTACA 
GCCACACAAC AAACGACATT AGCCTATGGT GACATGAACC ATGAGTGGAT TGGCAATGAG 
TGGCTGCCAA GCCTGGGGCT CCCTCAGTAC CGCAGCTACT TCATGGAATG TCTGGTGGAT 
GCTCGGATGC TGGATCACTT AACTAAAAAA GACCTGCGTG GGCAGCTGAA AATGGTGGAC 
AGCTTTCACA GAAACAGTTT TCAGTGTGGA ATTATGTGCC TCCGAAGACT GAATTACGAT 
CGAAAAGAGC TAGAAAGAAA AAGAGAAGAA AGCCAGAATG AAATTAAGGA TGTCCTTGTG 
TGGAGCAATG AGAGGATGAT CCACTGGGTC ATGTCCATTG GCCTTAAGGA ATATGCGAAC 
AACCTGCTCG AGAGCGGAGT TCACGGTGCA CTTGTGGCCT TAGATGAATC CTTTGATTAC 
AATGCATTAG CTCTCCTGCT GCAAATCCCC ACTCAAAACA CACAGGCTCG TGCTGTTCTG 
GAGAGGGAAT TCAATAACCT TCTTGCTATG GGCACTGACA GAAGACTTGA TGAAGATGAT 
GATAAGAGCT TTAGGAGAGC ACCTTCGTGG AGAAAGAAGT TTAGACCAAA GGACATAAGA 
GGTTTAGCTA CTGGATCAGC AGAGACTCTT CCTGCAAATT TCAGAGTTAC AACCTCAATG 
TCTTCACCCT CTATGCAGCC AAAGAAGATG CAGATTGATG GCAGTGTATC AGGAACACAA 
AGATTGGATT CTGCTACAGT AAGGACCTAC TCCTGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	PPFIA1
Entrez Gene ID	107206047
Full Name	PTPRF interacting protein alpha 1
Gene Type	protein-coding
Organism	Parus major(Great Tit)

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

PPFIA1 cDNA ORF clone, Parus major(Great Tit)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

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Target ORF information:

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Target ORF information:

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