FREM1 cDNA ORF clone, Pan troglodytes(chimpanzee)

The following FREM1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the FREM1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OPc12157 XM_009456320.1
Latest version!
Pan troglodytes FRAS1 related extracellular matrix 1 (FREM1), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OPc12157 XM_009456321.2
Latest version!
Pan troglodytes FRAS1 related extracellular matrix 1 (FREM1), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OPc109245 XM_016960630.2
Latest version!
Pan troglodytes FRAS1 related extracellular matrix 1 (FREM1), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OPc109246 XM_024345573.1
Latest version!
Pan troglodytes FRAS1 related extracellular matrix 1 (FREM1), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OPc88759 XM_016960630.1
Latest version!
Pan troglodytes FRAS1 related extracellular matrix 1 (FREM1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OPc88760 XM_016960631.1
Latest version!
Pan troglodytes FRAS1 related extracellular matrix 1 (FREM1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OPc109241 XM_024345569.1
Latest version!
Pan troglodytes FRAS1 related extracellular matrix 1 (FREM1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OPc109241 XM_024345566.1
Latest version!
Pan troglodytes FRAS1 related extracellular matrix 1 (FREM1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OPc109241 XM_024345565.1
Latest version!
Pan troglodytes FRAS1 related extracellular matrix 1 (FREM1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OPc109241 XM_024345568.1
Latest version!
Pan troglodytes FRAS1 related extracellular matrix 1 (FREM1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OPc109241 XM_024345567.1
Latest version!
Pan troglodytes FRAS1 related extracellular matrix 1 (FREM1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OPc109242 XM_024345570.1
Latest version!
Pan troglodytes FRAS1 related extracellular matrix 1 (FREM1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OPc109243 XM_009456318.3
Latest version!
Pan troglodytes FRAS1 related extracellular matrix 1 (FREM1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OPc109243 XM_024345571.1
Latest version!
Pan troglodytes FRAS1 related extracellular matrix 1 (FREM1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OPc109244 XM_024345572.1
Latest version!
Pan troglodytes FRAS1 related extracellular matrix 1 (FREM1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OPc88756 XM_016960626.1
Latest version!
Pan troglodytes FRAS1 related extracellular matrix 1 (FREM1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
OPc88756 XM_016960625.1
Latest version!
Pan troglodytes FRAS1 related extracellular matrix 1 (FREM1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
OPc88756 XM_016960627.1
Latest version!
Pan troglodytes FRAS1 related extracellular matrix 1 (FREM1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
OPc88756 XM_009456318.2
Latest version!
Pan troglodytes FRAS1 related extracellular matrix 1 (FREM1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
OPc88757 XM_016960628.1
Latest version!
Pan troglodytes FRAS1 related extracellular matrix 1 (FREM1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
OPc88758 XM_016960629.1
Latest version!
Pan troglodytes FRAS1 related extracellular matrix 1 (FREM1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
View Old Accession Versions >>
OPc12157 XM_009456321.1 Pan troglodytes FRAS1 related extracellular matrix 1 (FREM1), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OPc12157
    Clone ID Related Accession (Same CDS sequence) XM_009456320.1 , XM_009456321.1 , XM_009456321.2
    Accession Version XM_009456320.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2148bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-03-19
    Organism Pan troglodytes(chimpanzee)
    Product FRAS1-related extracellular matrix protein 1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036888.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGTGACAC AAGAATCCAT GCTGAAGGCT GCCTTGCCTC TCTTTACCAG ATTCATCATC 
    AGCAATGGAC TGCGGACCGA GCACGGGGTG TTTGAGATCA CACTGGAGAC TGTGGACAGA
    GCCCTGCCTG TGGTAACCAG GAACAAGGGG TTGAGACTGG CCCAAGGGGC CGTGGGCCTG
    CTTTCCCCTG ACCTCCTTCA GCTGACCGAC CCTGATACAC CTGTGGAGAA CCTCACCTTC
    CTCTTGGTTC AGCTTCCCCA GCATGGCCAG CTCTACCTGT GGGGGACAGG GCTACTTCAA
    CACAATTTCA CCCAGCAGGA TGTGGACAGC AGGAATGTGG CCTATCGGCA CTCAGGAGGG
    GACTCCCAGA CTGACTGCTT TACTTTCATG GCCACAGACG GGACAAACCA AGGCTTTATT
    GTGAATGGGA GAGTATGGGA AGAACCTGTT TTATTCACCA TTCAGGTAGA CCAATTGGAC
    AAAACAGCTC CTCGTATCAC ACTCTTGCAT TCCCCTTCTC AAGTGGGGCT CCTGAAAAAT
    GGCTGCTACG GGATTTACAT CACTTCCCGC GTGTTGAAGG CATCAGACCC TGACACTGAG
    GACGATCAGA TCATCTTTAA AATTCTACAA GGCCCAAAAC ATGGACATCT GGAGAACACA
    ACAACAGGTG AATTTATCCA TGAGAAATTT AGCCAAAAGG ACTTAAACAG TAAGACTATT
    CTTTACATCA TAAACCCATC TTTGGAAGTA AATTCAGATA CCGTGGAATT TCAAATCATG
    GACCCCACAG GGAACTCGGC CACTCCTCAA ATTTTGGAAC TGAAGTGGTC TCATATTGAA
    TGGTCACAGA CCGAATATGA AGTCTGTGAG AATGTGGGTG TGTTGCCCTT GGAAATTATC
    AGAAGGGGAT ATTCCATGGA CTCGGCCTTT GTGGGTATAA AGGTCAACCA AGTGTCAGCT
    GCAGATGGAA AAGATTTCAC CGTGATTCCA TCTAAACTGA TTCAGTTTGA CCCAGGAATG
    TCAACTAAGA TGTGGAATAT AGCAATTACC TATGACGGAT TAGAGGAAGA TGATGAGGTC
    TTTGAAGTAA TTCTGAACTC CCCTGTGAAT GCAGTTCTTG GCACAAAGAC AAAAGCTGCA
    GTGAAAATTT TGGACTCAAA AGGAGGACAA TGCCATCCTT CATATTCCTC CAACCAAAGC
    AAGCACAGCA CATGGGAGAA GGGCATTTGG CATCTGCTGC CCCCAGGGTC TTCCTCATCC
    ACCACTTCTG GTTCCTTTCA TCTGGAAAGA AGACCTCTTC CATCTTCCAT GCAGCTAGCA
    GTCACCAGGG GAGACACCCT GCGGGGCTTT GATTCTACAG ATCTTTCTCA AAGGAAGCTT
    AGGACCCGTG GGAATGGCAA AACAGTTCGT CCATCCTCTG TTTATAGAAA TGGAACAGAC
    ATCATCTATA ATTATCATGG GATAGTTTCC TTGAAACTAG AGGATGACAG CTTCCCAACT
    CACAAAAGGA AGGCCAAAGT ATCCATCATT AGTCAGCCAC AAAAGACAAT CAAAGTGGCA
    GAACTGCCTC AAGCAGATAA GGTGGAATCC ACAACTGACT CACACTTCCC CAGACAGGAC
    CAGTTGCCCT CATTTCCAAA GAACTGCACT CTGGAATTAA AGGGACTCTT CCATTTTGAA
    GAAGGCATCC AGAAGCTGTA TCAGTGCAAT GGGATTGCCT GGAAAGCCTG GAGTCCCCAA
    ACCAAGGATG TGGAAGAAAA ATCCTGTCCA GCCGGGTGGC ACCAGCACTC AGGCTACTGT
    CACATCTTGA TCACAGAGCA GAAAGGCACC TGGAATGCGG CTGCCCAAGC TTGCAGGGAA
    CAATACCTGG GCAACCTTGT AACTGTATTC TCCAGGCAGC ACATGCGGTG GCTCTGGGAC
    ATTGGTGAGA GAAAGTCCTT TTGGATAGGT TTGAACGACC AAGTGCATGC TGGCCACTGG
    GAGTGGATCG GTGGTGAACC TGTTGCCTTC ACCAATGGGA GAAGAGGGCC CTCTCAACGC
    TCCAAGCTTG GAAAGAGCTG TGTTTTGGTT CAAAGACAAG GGAAATGGCA AACAAAAGAT
    TGTAGGAGAG CCAAACCTCA TAATTATGTG TGTTCCAGAA AACTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009454595.1
    CDS94..2241
    Translation

    Target ORF information:

    RefSeq Version XM_009456320.1
    Organism Pan troglodytes(chimpanzee)
    Definition Pan troglodytes FRAS1 related extracellular matrix 1 (FREM1), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009456320.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGTGACAC AAGAATCCAT GCTGAAGGCT GCCTTGCCTC TCTTTACCAG ATTCATCATC 
    AGCAATGGAC TGCGGACCGA GCACGGGGTG TTTGAGATCA CACTGGAGAC TGTGGACAGA
    GCCCTGCCTG TGGTAACCAG GAACAAGGGG TTGAGACTGG CCCAAGGGGC CGTGGGCCTG
    CTTTCCCCTG ACCTCCTTCA GCTGACCGAC CCTGATACAC CTGTGGAGAA CCTCACCTTC
    CTCTTGGTTC AGCTTCCCCA GCATGGCCAG CTCTACCTGT GGGGGACAGG GCTACTTCAA
    CACAATTTCA CCCAGCAGGA TGTGGACAGC AGGAATGTGG CCTATCGGCA CTCAGGAGGG
    GACTCCCAGA CTGACTGCTT TACTTTCATG GCCACAGACG GGACAAACCA AGGCTTTATT
    GTGAATGGGA GAGTATGGGA AGAACCTGTT TTATTCACCA TTCAGGTAGA CCAATTGGAC
    AAAACAGCTC CTCGTATCAC ACTCTTGCAT TCCCCTTCTC AAGTGGGGCT CCTGAAAAAT
    GGCTGCTACG GGATTTACAT CACTTCCCGC GTGTTGAAGG CATCAGACCC TGACACTGAG
    GACGATCAGA TCATCTTTAA AATTCTACAA GGCCCAAAAC ATGGACATCT GGAGAACACA
    ACAACAGGTG AATTTATCCA TGAGAAATTT AGCCAAAAGG ACTTAAACAG TAAGACTATT
    CTTTACATCA TAAACCCATC TTTGGAAGTA AATTCAGATA CCGTGGAATT TCAAATCATG
    GACCCCACAG GGAACTCGGC CACTCCTCAA ATTTTGGAAC TGAAGTGGTC TCATATTGAA
    TGGTCACAGA CCGAATATGA AGTCTGTGAG AATGTGGGTG TGTTGCCCTT GGAAATTATC
    AGAAGGGGAT ATTCCATGGA CTCGGCCTTT GTGGGTATAA AGGTCAACCA AGTGTCAGCT
    GCAGATGGAA AAGATTTCAC CGTGATTCCA TCTAAACTGA TTCAGTTTGA CCCAGGAATG
    TCAACTAAGA TGTGGAATAT AGCAATTACC TATGACGGAT TAGAGGAAGA TGATGAGGTC
    TTTGAAGTAA TTCTGAACTC CCCTGTGAAT GCAGTTCTTG GCACAAAGAC AAAAGCTGCA
    GTGAAAATTT TGGACTCAAA AGGAGGACAA TGCCATCCTT CATATTCCTC CAACCAAAGC
    AAGCACAGCA CATGGGAGAA GGGCATTTGG CATCTGCTGC CCCCAGGGTC TTCCTCATCC
    ACCACTTCTG GTTCCTTTCA TCTGGAAAGA AGACCTCTTC CATCTTCCAT GCAGCTAGCA
    GTCACCAGGG GAGACACCCT GCGGGGCTTT GATTCTACAG ATCTTTCTCA AAGGAAGCTT
    AGGACCCGTG GGAATGGCAA AACAGTTCGT CCATCCTCTG TTTATAGAAA TGGAACAGAC
    ATCATCTATA ATTATCATGG GATAGTTTCC TTGAAACTAG AGGATGACAG CTTCCCAACT
    CACAAAAGGA AGGCCAAAGT ATCCATCATT AGTCAGCCAC AAAAGACAAT CAAAGTGGCA
    GAACTGCCTC AAGCAGATAA GGTGGAATCC ACAACTGACT CACACTTCCC CAGACAGGAC
    CAGTTGCCCT CATTTCCAAA GAACTGCACT CTGGAATTAA AGGGACTCTT CCATTTTGAA
    GAAGGCATCC AGAAGCTGTA TCAGTGCAAT GGGATTGCCT GGAAAGCCTG GAGTCCCCAA
    ACCAAGGATG TGGAAGAAAA ATCCTGTCCA GCCGGGTGGC ACCAGCACTC AGGCTACTGT
    CACATCTTGA TCACAGAGCA GAAAGGCACC TGGAATGCGG CTGCCCAAGC TTGCAGGGAA
    CAATACCTGG GCAACCTTGT AACTGTATTC TCCAGGCAGC ACATGCGGTG GCTCTGGGAC
    ATTGGTGAGA GAAAGTCCTT TTGGATAGGT TTGAACGACC AAGTGCATGC TGGCCACTGG
    GAGTGGATCG GTGGTGAACC TGTTGCCTTC ACCAATGGGA GAAGAGGGCC CTCTCAACGC
    TCCAAGCTTG GAAAGAGCTG TGTTTTGGTT CAAAGACAAG GGAAATGGCA AACAAAAGAT
    TGTAGGAGAG CCAAACCTCA TAATTATGTG TGTTCCAGAA AACTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OPc12157
    Clone ID Related Accession (Same CDS sequence) XM_009456320.1 , XM_009456321.1 , XM_009456321.2
    Accession Version XM_009456321.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2148bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-01
    Organism Pan troglodytes(chimpanzee)
    Product FRAS1-related extracellular matrix protein 1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006476.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGTGACAC AAGAATCCAT GCTGAAGGCT GCCTTGCCTC TCTTTACCAG ATTCATCATC 
    AGCAATGGAC TGCGGACCGA GCACGGGGTG TTTGAGATCA CACTGGAGAC TGTGGACAGA
    GCCCTGCCTG TGGTAACCAG GAACAAGGGG TTGAGACTGG CCCAAGGGGC CGTGGGCCTG
    CTTTCCCCTG ACCTCCTTCA GCTGACCGAC CCTGATACAC CTGTGGAGAA CCTCACCTTC
    CTCTTGGTTC AGCTTCCCCA GCATGGCCAG CTCTACCTGT GGGGGACAGG GCTACTTCAA
    CACAATTTCA CCCAGCAGGA TGTGGACAGC AGGAATGTGG CCTATCGGCA CTCAGGAGGG
    GACTCCCAGA CTGACTGCTT TACTTTCATG GCCACAGACG GGACAAACCA AGGCTTTATT
    GTGAATGGGA GAGTATGGGA AGAACCTGTT TTATTCACCA TTCAGGTAGA CCAATTGGAC
    AAAACAGCTC CTCGTATCAC ACTCTTGCAT TCCCCTTCTC AAGTGGGGCT CCTGAAAAAT
    GGCTGCTACG GGATTTACAT CACTTCCCGC GTGTTGAAGG CATCAGACCC TGACACTGAG
    GACGATCAGA TCATCTTTAA AATTCTACAA GGCCCAAAAC ATGGACATCT GGAGAACACA
    ACAACAGGTG AATTTATCCA TGAGAAATTT AGCCAAAAGG ACTTAAACAG TAAGACTATT
    CTTTACATCA TAAACCCATC TTTGGAAGTA AATTCAGATA CCGTGGAATT TCAAATCATG
    GACCCCACAG GGAACTCGGC CACTCCTCAA ATTTTGGAAC TGAAGTGGTC TCATATTGAA
    TGGTCACAGA CCGAATATGA AGTCTGTGAG AATGTGGGTG TGTTGCCCTT GGAAATTATC
    AGAAGGGGAT ATTCCATGGA CTCGGCCTTT GTGGGTATAA AGGTCAACCA AGTGTCAGCT
    GCAGATGGAA AAGATTTCAC CGTGATTCCA TCTAAACTGA TTCAGTTTGA CCCAGGAATG
    TCAACTAAGA TGTGGAATAT AGCAATTACC TATGACGGAT TAGAGGAAGA TGATGAGGTC
    TTTGAAGTAA TTCTGAACTC CCCTGTGAAT GCAGTTCTTG GCACAAAGAC AAAAGCTGCA
    GTGAAAATTT TGGACTCAAA AGGAGGACAA TGCCATCCTT CATATTCCTC CAACCAAAGC
    AAGCACAGCA CATGGGAGAA GGGCATTTGG CATCTGCTGC CCCCAGGGTC TTCCTCATCC
    ACCACTTCTG GTTCCTTTCA TCTGGAAAGA AGACCTCTTC CATCTTCCAT GCAGCTAGCA
    GTCACCAGGG GAGACACCCT GCGGGGCTTT GATTCTACAG ATCTTTCTCA AAGGAAGCTT
    AGGACCCGTG GGAATGGCAA AACAGTTCGT CCATCCTCTG TTTATAGAAA TGGAACAGAC
    ATCATCTATA ATTATCATGG GATAGTTTCC TTGAAACTAG AGGATGACAG CTTCCCAACT
    CACAAAAGGA AGGCCAAAGT ATCCATCATT AGTCAGCCAC AAAAGACAAT CAAAGTGGCA
    GAACTGCCTC AAGCAGATAA GGTGGAATCC ACAACTGACT CACACTTCCC CAGACAGGAC
    CAGTTGCCCT CATTTCCAAA GAACTGCACT CTGGAATTAA AGGGACTCTT CCATTTTGAA
    GAAGGCATCC AGAAGCTGTA TCAGTGCAAT GGGATTGCCT GGAAAGCCTG GAGTCCCCAA
    ACCAAGGATG TGGAAGAAAA ATCCTGTCCA GCCGGGTGGC ACCAGCACTC AGGCTACTGT
    CACATCTTGA TCACAGAGCA GAAAGGCACC TGGAATGCGG CTGCCCAAGC TTGCAGGGAA
    CAATACCTGG GCAACCTTGT AACTGTATTC TCCAGGCAGC ACATGCGGTG GCTCTGGGAC
    ATTGGTGAGA GAAAGTCCTT TTGGATAGGT TTGAACGACC AAGTGCATGC TGGCCACTGG
    GAGTGGATCG GTGGTGAACC TGTTGCCTTC ACCAATGGGA GAAGAGGGCC CTCTCAACGC
    TCCAAGCTTG GAAAGAGCTG TGTTTTGGTT CAAAGACAAG GGAAATGGCA AACAAAAGAT
    TGTAGGAGAG CCAAACCTCA TAATTATGTG TGTTCCAGAA AACTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009454596.1
    CDS89..2236
    Translation

    Target ORF information:

    RefSeq Version XM_009456321.1
    Organism Pan troglodytes(chimpanzee)
    Definition Pan troglodytes FRAS1 related extracellular matrix 1 (FREM1), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009456321.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGTGACAC AAGAATCCAT GCTGAAGGCT GCCTTGCCTC TCTTTACCAG ATTCATCATC 
    AGCAATGGAC TGCGGACCGA GCACGGGGTG TTTGAGATCA CACTGGAGAC TGTGGACAGA
    GCCCTGCCTG TGGTAACCAG GAACAAGGGG TTGAGACTGG CCCAAGGGGC CGTGGGCCTG
    CTTTCCCCTG ACCTCCTTCA GCTGACCGAC CCTGATACAC CTGTGGAGAA CCTCACCTTC
    CTCTTGGTTC AGCTTCCCCA GCATGGCCAG CTCTACCTGT GGGGGACAGG GCTACTTCAA
    CACAATTTCA CCCAGCAGGA TGTGGACAGC AGGAATGTGG CCTATCGGCA CTCAGGAGGG
    GACTCCCAGA CTGACTGCTT TACTTTCATG GCCACAGACG GGACAAACCA AGGCTTTATT
    GTGAATGGGA GAGTATGGGA AGAACCTGTT TTATTCACCA TTCAGGTAGA CCAATTGGAC
    AAAACAGCTC CTCGTATCAC ACTCTTGCAT TCCCCTTCTC AAGTGGGGCT CCTGAAAAAT
    GGCTGCTACG GGATTTACAT CACTTCCCGC GTGTTGAAGG CATCAGACCC TGACACTGAG
    GACGATCAGA TCATCTTTAA AATTCTACAA GGCCCAAAAC ATGGACATCT GGAGAACACA
    ACAACAGGTG AATTTATCCA TGAGAAATTT AGCCAAAAGG ACTTAAACAG TAAGACTATT
    CTTTACATCA TAAACCCATC TTTGGAAGTA AATTCAGATA CCGTGGAATT TCAAATCATG
    GACCCCACAG GGAACTCGGC CACTCCTCAA ATTTTGGAAC TGAAGTGGTC TCATATTGAA
    TGGTCACAGA CCGAATATGA AGTCTGTGAG AATGTGGGTG TGTTGCCCTT GGAAATTATC
    AGAAGGGGAT ATTCCATGGA CTCGGCCTTT GTGGGTATAA AGGTCAACCA AGTGTCAGCT
    GCAGATGGAA AAGATTTCAC CGTGATTCCA TCTAAACTGA TTCAGTTTGA CCCAGGAATG
    TCAACTAAGA TGTGGAATAT AGCAATTACC TATGACGGAT TAGAGGAAGA TGATGAGGTC
    TTTGAAGTAA TTCTGAACTC CCCTGTGAAT GCAGTTCTTG GCACAAAGAC AAAAGCTGCA
    GTGAAAATTT TGGACTCAAA AGGAGGACAA TGCCATCCTT CATATTCCTC CAACCAAAGC
    AAGCACAGCA CATGGGAGAA GGGCATTTGG CATCTGCTGC CCCCAGGGTC TTCCTCATCC
    ACCACTTCTG GTTCCTTTCA TCTGGAAAGA AGACCTCTTC CATCTTCCAT GCAGCTAGCA
    GTCACCAGGG GAGACACCCT GCGGGGCTTT GATTCTACAG ATCTTTCTCA AAGGAAGCTT
    AGGACCCGTG GGAATGGCAA AACAGTTCGT CCATCCTCTG TTTATAGAAA TGGAACAGAC
    ATCATCTATA ATTATCATGG GATAGTTTCC TTGAAACTAG AGGATGACAG CTTCCCAACT
    CACAAAAGGA AGGCCAAAGT ATCCATCATT AGTCAGCCAC AAAAGACAAT CAAAGTGGCA
    GAACTGCCTC AAGCAGATAA GGTGGAATCC ACAACTGACT CACACTTCCC CAGACAGGAC
    CAGTTGCCCT CATTTCCAAA GAACTGCACT CTGGAATTAA AGGGACTCTT CCATTTTGAA
    GAAGGCATCC AGAAGCTGTA TCAGTGCAAT GGGATTGCCT GGAAAGCCTG GAGTCCCCAA
    ACCAAGGATG TGGAAGAAAA ATCCTGTCCA GCCGGGTGGC ACCAGCACTC AGGCTACTGT
    CACATCTTGA TCACAGAGCA GAAAGGCACC TGGAATGCGG CTGCCCAAGC TTGCAGGGAA
    CAATACCTGG GCAACCTTGT AACTGTATTC TCCAGGCAGC ACATGCGGTG GCTCTGGGAC
    ATTGGTGAGA GAAAGTCCTT TTGGATAGGT TTGAACGACC AAGTGCATGC TGGCCACTGG
    GAGTGGATCG GTGGTGAACC TGTTGCCTTC ACCAATGGGA GAAGAGGGCC CTCTCAACGC
    TCCAAGCTTG GAAAGAGCTG TGTTTTGGTT CAAAGACAAG GGAAATGGCA AACAAAAGAT
    TGTAGGAGAG CCAAACCTCA TAATTATGTG TGTTCCAGAA AACTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OPc12157
    Clone ID Related Accession (Same CDS sequence) XM_009456320.1 , XM_009456321.1 , XM_009456321.2
    Accession Version XM_009456321.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2148bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-03-19
    Organism Pan troglodytes(chimpanzee)
    Product FRAS1-related extracellular matrix protein 1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036888.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_009456321.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGTGACAC AAGAATCCAT GCTGAAGGCT GCCTTGCCTC TCTTTACCAG ATTCATCATC 
    AGCAATGGAC TGCGGACCGA GCACGGGGTG TTTGAGATCA CACTGGAGAC TGTGGACAGA
    GCCCTGCCTG TGGTAACCAG GAACAAGGGG TTGAGACTGG CCCAAGGGGC CGTGGGCCTG
    CTTTCCCCTG ACCTCCTTCA GCTGACCGAC CCTGATACAC CTGTGGAGAA CCTCACCTTC
    CTCTTGGTTC AGCTTCCCCA GCATGGCCAG CTCTACCTGT GGGGGACAGG GCTACTTCAA
    CACAATTTCA CCCAGCAGGA TGTGGACAGC AGGAATGTGG CCTATCGGCA CTCAGGAGGG
    GACTCCCAGA CTGACTGCTT TACTTTCATG GCCACAGACG GGACAAACCA AGGCTTTATT
    GTGAATGGGA GAGTATGGGA AGAACCTGTT TTATTCACCA TTCAGGTAGA CCAATTGGAC
    AAAACAGCTC CTCGTATCAC ACTCTTGCAT TCCCCTTCTC AAGTGGGGCT CCTGAAAAAT
    GGCTGCTACG GGATTTACAT CACTTCCCGC GTGTTGAAGG CATCAGACCC TGACACTGAG
    GACGATCAGA TCATCTTTAA AATTCTACAA GGCCCAAAAC ATGGACATCT GGAGAACACA
    ACAACAGGTG AATTTATCCA TGAGAAATTT AGCCAAAAGG ACTTAAACAG TAAGACTATT
    CTTTACATCA TAAACCCATC TTTGGAAGTA AATTCAGATA CCGTGGAATT TCAAATCATG
    GACCCCACAG GGAACTCGGC CACTCCTCAA ATTTTGGAAC TGAAGTGGTC TCATATTGAA
    TGGTCACAGA CCGAATATGA AGTCTGTGAG AATGTGGGTG TGTTGCCCTT GGAAATTATC
    AGAAGGGGAT ATTCCATGGA CTCGGCCTTT GTGGGTATAA AGGTCAACCA AGTGTCAGCT
    GCAGATGGAA AAGATTTCAC CGTGATTCCA TCTAAACTGA TTCAGTTTGA CCCAGGAATG
    TCAACTAAGA TGTGGAATAT AGCAATTACC TATGACGGAT TAGAGGAAGA TGATGAGGTC
    TTTGAAGTAA TTCTGAACTC CCCTGTGAAT GCAGTTCTTG GCACAAAGAC AAAAGCTGCA
    GTGAAAATTT TGGACTCAAA AGGAGGACAA TGCCATCCTT CATATTCCTC CAACCAAAGC
    AAGCACAGCA CATGGGAGAA GGGCATTTGG CATCTGCTGC CCCCAGGGTC TTCCTCATCC
    ACCACTTCTG GTTCCTTTCA TCTGGAAAGA AGACCTCTTC CATCTTCCAT GCAGCTAGCA
    GTCACCAGGG GAGACACCCT GCGGGGCTTT GATTCTACAG ATCTTTCTCA AAGGAAGCTT
    AGGACCCGTG GGAATGGCAA AACAGTTCGT CCATCCTCTG TTTATAGAAA TGGAACAGAC
    ATCATCTATA ATTATCATGG GATAGTTTCC TTGAAACTAG AGGATGACAG CTTCCCAACT
    CACAAAAGGA AGGCCAAAGT ATCCATCATT AGTCAGCCAC AAAAGACAAT CAAAGTGGCA
    GAACTGCCTC AAGCAGATAA GGTGGAATCC ACAACTGACT CACACTTCCC CAGACAGGAC
    CAGTTGCCCT CATTTCCAAA GAACTGCACT CTGGAATTAA AGGGACTCTT CCATTTTGAA
    GAAGGCATCC AGAAGCTGTA TCAGTGCAAT GGGATTGCCT GGAAAGCCTG GAGTCCCCAA
    ACCAAGGATG TGGAAGAAAA ATCCTGTCCA GCCGGGTGGC ACCAGCACTC AGGCTACTGT
    CACATCTTGA TCACAGAGCA GAAAGGCACC TGGAATGCGG CTGCCCAAGC TTGCAGGGAA
    CAATACCTGG GCAACCTTGT AACTGTATTC TCCAGGCAGC ACATGCGGTG GCTCTGGGAC
    ATTGGTGAGA GAAAGTCCTT TTGGATAGGT TTGAACGACC AAGTGCATGC TGGCCACTGG
    GAGTGGATCG GTGGTGAACC TGTTGCCTTC ACCAATGGGA GAAGAGGGCC CTCTCAACGC
    TCCAAGCTTG GAAAGAGCTG TGTTTTGGTT CAAAGACAAG GGAAATGGCA AACAAAAGAT
    TGTAGGAGAG CCAAACCTCA TAATTATGTG TGTTCCAGAA AACTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009454596.1
    CDS88..2235
    Translation

    Target ORF information:

    RefSeq Version XM_009456321.2
    Organism Pan troglodytes(chimpanzee)
    Definition Pan troglodytes FRAS1 related extracellular matrix 1 (FREM1), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009456321.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGTGACAC AAGAATCCAT GCTGAAGGCT GCCTTGCCTC TCTTTACCAG ATTCATCATC 
    AGCAATGGAC TGCGGACCGA GCACGGGGTG TTTGAGATCA CACTGGAGAC TGTGGACAGA
    GCCCTGCCTG TGGTAACCAG GAACAAGGGG TTGAGACTGG CCCAAGGGGC CGTGGGCCTG
    CTTTCCCCTG ACCTCCTTCA GCTGACCGAC CCTGATACAC CTGTGGAGAA CCTCACCTTC
    CTCTTGGTTC AGCTTCCCCA GCATGGCCAG CTCTACCTGT GGGGGACAGG GCTACTTCAA
    CACAATTTCA CCCAGCAGGA TGTGGACAGC AGGAATGTGG CCTATCGGCA CTCAGGAGGG
    GACTCCCAGA CTGACTGCTT TACTTTCATG GCCACAGACG GGACAAACCA AGGCTTTATT
    GTGAATGGGA GAGTATGGGA AGAACCTGTT TTATTCACCA TTCAGGTAGA CCAATTGGAC
    AAAACAGCTC CTCGTATCAC ACTCTTGCAT TCCCCTTCTC AAGTGGGGCT CCTGAAAAAT
    GGCTGCTACG GGATTTACAT CACTTCCCGC GTGTTGAAGG CATCAGACCC TGACACTGAG
    GACGATCAGA TCATCTTTAA AATTCTACAA GGCCCAAAAC ATGGACATCT GGAGAACACA
    ACAACAGGTG AATTTATCCA TGAGAAATTT AGCCAAAAGG ACTTAAACAG TAAGACTATT
    CTTTACATCA TAAACCCATC TTTGGAAGTA AATTCAGATA CCGTGGAATT TCAAATCATG
    GACCCCACAG GGAACTCGGC CACTCCTCAA ATTTTGGAAC TGAAGTGGTC TCATATTGAA
    TGGTCACAGA CCGAATATGA AGTCTGTGAG AATGTGGGTG TGTTGCCCTT GGAAATTATC
    AGAAGGGGAT ATTCCATGGA CTCGGCCTTT GTGGGTATAA AGGTCAACCA AGTGTCAGCT
    GCAGATGGAA AAGATTTCAC CGTGATTCCA TCTAAACTGA TTCAGTTTGA CCCAGGAATG
    TCAACTAAGA TGTGGAATAT AGCAATTACC TATGACGGAT TAGAGGAAGA TGATGAGGTC
    TTTGAAGTAA TTCTGAACTC CCCTGTGAAT GCAGTTCTTG GCACAAAGAC AAAAGCTGCA
    GTGAAAATTT TGGACTCAAA AGGAGGACAA TGCCATCCTT CATATTCCTC CAACCAAAGC
    AAGCACAGCA CATGGGAGAA GGGCATTTGG CATCTGCTGC CCCCAGGGTC TTCCTCATCC
    ACCACTTCTG GTTCCTTTCA TCTGGAAAGA AGACCTCTTC CATCTTCCAT GCAGCTAGCA
    GTCACCAGGG GAGACACCCT GCGGGGCTTT GATTCTACAG ATCTTTCTCA AAGGAAGCTT
    AGGACCCGTG GGAATGGCAA AACAGTTCGT CCATCCTCTG TTTATAGAAA TGGAACAGAC
    ATCATCTATA ATTATCATGG GATAGTTTCC TTGAAACTAG AGGATGACAG CTTCCCAACT
    CACAAAAGGA AGGCCAAAGT ATCCATCATT AGTCAGCCAC AAAAGACAAT CAAAGTGGCA
    GAACTGCCTC AAGCAGATAA GGTGGAATCC ACAACTGACT CACACTTCCC CAGACAGGAC
    CAGTTGCCCT CATTTCCAAA GAACTGCACT CTGGAATTAA AGGGACTCTT CCATTTTGAA
    GAAGGCATCC AGAAGCTGTA TCAGTGCAAT GGGATTGCCT GGAAAGCCTG GAGTCCCCAA
    ACCAAGGATG TGGAAGAAAA ATCCTGTCCA GCCGGGTGGC ACCAGCACTC AGGCTACTGT
    CACATCTTGA TCACAGAGCA GAAAGGCACC TGGAATGCGG CTGCCCAAGC TTGCAGGGAA
    CAATACCTGG GCAACCTTGT AACTGTATTC TCCAGGCAGC ACATGCGGTG GCTCTGGGAC
    ATTGGTGAGA GAAAGTCCTT TTGGATAGGT TTGAACGACC AAGTGCATGC TGGCCACTGG
    GAGTGGATCG GTGGTGAACC TGTTGCCTTC ACCAATGGGA GAAGAGGGCC CTCTCAACGC
    TCCAAGCTTG GAAAGAGCTG TGTTTTGGTT CAAAGACAAG GGAAATGGCA AACAAAAGAT
    TGTAGGAGAG CCAAACCTCA TAATTATGTG TGTTCCAGAA AACTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OPc109245
    Clone ID Related Accession (Same CDS sequence) XM_016960630.2
    Accession Version XM_016960630.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5643bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-03-19
    Organism Pan troglodytes(chimpanzee)
    Product FRAS1-related extracellular matrix protein 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036888.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_016960630.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGGCCATGT TTATTCTGGA AGTGGATCAG TTCATCCTGA CCTCCTTGAC TACATCAGTT 
    CTGGACTGTG AAGAAGATGA GACCCCTAAA CCCTTGCTGG TGTTCAACAT TACTAAAGCC
    CCGCTCCAGG GCTATGTGAC TCACCTGTTG GATCACACCA GACCAATCTC CTCATTCACC
    TGGAAAGATC TCAGTGACAT GCAGATCGCC TATCAGCCAC CAAACAGCAG CCATTCTGAG
    AGGAGACATG ATGAGGTAGA ATTGGAGGTA TACGACTTCT TCTTTGAAAG GAGTGCACCT
    ATGACAGTCC ACATCTCCAT CAGAACAGCA GATACAAATG CCCCCCGTGT ATCCTGGAAT
    ACAGGTCTGA ATCTCCTTGA GGGGCAGTCT CGAGCCATCA CTTGGGAACA GTTTCAGGTT
    GTCGACAATG ACGACATCGG TGCTGTCCGG CTAGTCACCG TTGGTGGCCT GCAGCATGGA
    TGGCTGACTT TAAGAGGGGG GAAAGGATTT CTCTTCACCG TGGCTGACCT CCAGGCTGGA
    GTTGTTCGCT ATCATCATGA TGACAGCGAC TCCACCAAAG ACTTCGTGGT CTTCCGGATA
    TTTGATGGCC ATCACAGCAT CCGTCACAAA TTCCCCATCA ACGTCTTGCC CAAAGATGAT
    AGTCCCCCGT TCCTCATAAC CAATGTTGTC ATTGAACTGG AGGAGGGGCA GACCATCCTG
    ATCCAGGGAT CCATGCTGCG AGCTTCAGAT GTGGATGCCA GTGATGACTA CATCTTCTTC
    AATATCACAA AGCCTCCACA GGCTGGGGAG ATCATGAAGA AGCCAGGGCC AGGACTGATA
    GGCTATCCTG TCCCTGGCTT CCTTCAGAGG GATTTGTTTA ATGGAATCAT TTATTATCGT
    CATTTTGGTG GAGAAATCTT TGAAGATTCT TTTCAATTTG TCCTGTGGGA CAGCCATGAA
    CCTCCAAATC TTTCAGTGCC ACAGGTGGCA ACAATCCATG TAACTCCAGT GGATGACCAG
    CTTCCAAAAG AGGCTCCTGG AGTTTCTCGG CATTTGGTTG TCAAGGAAAC TGAGGTGGCC
    TATATAACTA AGAAACAGCT ACATTTTATA GATTCAGAAT CATATGACAG GGAGCTGGTC
    TACACGATAA CTACTCCTCC ATTTTTCTCC TTCAGCCACA GACACTTGGA TGCTGGGAAA
    TTATTTATGG TGGACAGCAT ACCAAAAGTA GTTAAGAATC CTACGGCCCT GGAGCTGAGG
    TCATTCACTC AGCATGCTGT GAACTATATG AAAGTGGCCT ACATGCCCCC CATGCAAGAC
    ATTGGTCCCC ATTGCAGAGA TGTCCAGTTC ACATTTTCTG TCAGTAACCA ACATGGCGGC
    ACTTTGCATG GGATCTGCTT TAACATTACA ATCCTCCCAG TGGACAATCA AGTTCCTGAG
    GCGTTCACCA ACCCTCTGAA AGTGACTGAG GGAGGTCAAA GCATCATCAG CACAGAGCAC
    ATTCTGATTT CTGATGCAGA TACCAAGCTG GACAATATTG ACCTCTCCCT GCGGGAATTG
    CCTCTGCACG GAAGGGTGGA GCTGAATGGA TTTCCTCTAA ATTCAGGGGG CACATTTTCT
    TGGGGCGATC TCCATACCTT AAAAGTTAGG TATCAACATG ATGGAACTGA AGTTCTTCAG
    GATGACCTAC TCTTGGAGGT CACCGATGGC ACAAATTCAG CAGAATTTGT TCTACATGTT
    GAGGTATTCC CTGTCAACGA TGAGCCACCA GTCTTAAAGG CTGACCTCAT GCCTGTCATG
    AATTGCTCAG AGGGAGGAGA GGTGGTCATC ACCTCTGAAT ACATTTTTGC TACTGATGTG
    GACAGCGATA ACTTGAAGTT GATGTTTGTG ATTGCTCGCG AACCTCAGCA TGGGGTGGTG
    AGGAGAGCTG GAGTCACAGT GGATCAGTTC TCTCAGAGAG ATGTTATCTC AGAGGCCGTG
    ACATACAAAC ACACAGGTGG CGAGATTGGC ATGATGCCCT GTTTTGACAC CATTACATTG
    GTGGTTTCGG ATGGAGAGGC TGGCCCTTTT GTGAATGGCT GTTGCTACAA TGGACCCAAT
    CCATCTGTTC CGCTTCATGC GTCCTTTCCA GTGTATGATC TCAACATCAC GGTATACCCA
    GTAGACAACC AGCCACCTTC CATTGCAATA GGTCCCATGT TTGTTGTAGA TGAGGGCTGC
    TCAACAGCCC TTACTGTTAA CCATTTGTCC GCCACTGACC CTGACACTGC AGCAGATGAC
    TTGGAATTTG TTTTGGTTTC TCCTCCTCAG TTTGGCTACC TCGAAAATAT ACTCCCTTCT
    GTGGGTTTTG AAAAAAGCAA TATTGGCATA AGTATAGATT CATTTCAGTG GAAAGACATG
    AACGCTTTTC ACATTAACTA TGTGCAGTCC AGGCATCTGA GGATAGAACC AACTGCCGAC
    CAGTTCACGG TGTACGTCAC AGATGGGAAG CAACACTCCT TGGAGATACC ATTTTCTATT
    ATAATCAACC CCACAAATGA TGAAGCTCCT GACTTTGTAG TGCAGAATAT TACTGTGTGC
    GAGGGTCAGA TGAAAGAGCT GGACTCTTCC ATCATCAGCG CTGTGGACCT GGACATTCCC
    CAGGATGCCC TGCTGTTCAG CATCACTCAA AAGCCACGCC ATGGCCTCCT CATCGATAGG
    GGGTTTAGCA AAGACTTCTC TGAGAATAAG CAGCCAGCCA ACCCTCACCA GAAACATGTA
    CCTGTTCACA GTTTTTCCAT GGAACTCCTC AAGACTGGAA TGAGGTTGAC GTACATGCAT
    GATGACTCAG AGAGCCTTGC TGATGATTTT ACAATCCGTT TGTCAGATGG GAAACATAAG
    ATACTTAAAA CCATTTCAAT AGAGGTCATC CCAGTTAATG ATGAAAAACC AATGCTGAGC
    AAGAAGGCTG AAATTGCAAT GAATATGGGT GAAACTCGTA TTATTTCCAG TGCTATTCTT
    TCAGCCATAG ATGAAGACTC ACCCAGGGAG AAGATTTACT ATGTATTTGA AAGGCTTCCC
    CAAAATGGGC AACTTCAGCT TAAGATAGGG AGGGACTGGG TTCCTCTCTC CCCTGGCATG
    AAATGCACTC AGGAGGAAGT GGATCTGAAC TTGCTGAGAT ACACACACAC CGGGGCAATG
    GATTCCCAGA ATCAAGATAG CTTCACCTTC TACCTTTGGG ATGGCAACAA CAGGTCCCCT
    GCTCTTGACT GTCAAATCAC CATCAAGGAC ATGGAAAAAG GTGATATTGT CATCCTTACA
    AAACCACTCG TGGTGTCTAA AGGTGATAGA GGTTTCTTAA CAACCACCAC TCTCCTGGCT
    GTGGACGGAA CAGACAAGCC TGAGGAACTG CTCTATGTCA TCACCTCCCC TCCACGATAT
    GGCCAGATCG AATATGTTCA CTATCCTGGA GTTCCCATTA CAAACTTCAG CCAAATGGAT
    ATAGCGGGGC AGACAGTTTG CTATGTACAC AAGAGCAAGG TGACTGTCTC CAGTGACAGA
    TTCAGATTCA TCATCAGCAA TGGACTGCGG ACCGAGCACG GGGTGTTTGA GATCACACTG
    GAGACTGTGG ACAGAGCCCT GCCTGTGGTA ACCAGGAACA AGGGGTTGAG ACTGGCCCAA
    GGGGCCGTGG GCCTGCTTTC CCCTGACCTC CTTCAGCTGA CCGACCCTGA TACACCTGTG
    GAGAACCTCA CCTTCCTCTT GGTTCAGCTT CCCCAGCATG GCCAGCTCTA CCTGTGGGGG
    ACAGGGCTAC TTCAACACAA TTTCACCCAG CAGGATGTGG ACAGCAGGAA TGTGGCCTAT
    CGGCACTCAG GAGGGGACTC CCAGACTGAC TGCTTTACTT TCATGGCCAC AGACGGGACA
    AACCAAGGCT TTATTGTGAA TGGGAGAGTA TGGGAAGAAC CTGTTTTATT CACCATTCAG
    GTAGACCAAT TGGACAAAAC AGCTCCTCGT ATCACACTCT TGCATTCCCC TTCTCAAGTG
    GGGCTCCTGA AAAATGGCTG CTACGGGATT TACATCACTT CCCGCGTGTT GAAGGCATCA
    GACCCTGACA CTGAGGACGA TCAGATCATC TTTAAAATTC TACAAGGCCC AAAACATGGA
    CATCTGGAGA ACACAACAAC AGGTGAATTT ATCCATGAGA AATTTAGCCA AAAGGACTTA
    AACAGTAAGA CTATTCTTTA CATCATAAAC CCATCTTTGG AAGTAAATTC AGATACCGTG
    GAATTTCAAA TCATGGACCC CACAGGGAAC TCGGCCACTC CTCAAATTTT GGAACTGAAG
    TGGTCTCATA TTGAATGGTC ACAGACCGAA TATGAAGTCT GTGAGAATGT GGGTGTGTTG
    CCCTTGGAAA TTATCAGAAG GGGATATTCC ATGGACTCGG CCTTTGTGGG TATAAAGGTC
    AACCAAGTGT CAGCTGCAGA TGGAAAAGAT TTCACCGTGA TTCCATCTAA ACTGATTCAG
    TTTGACCCAG GAATGTCAAC TAAGATGTGG AATATAGCAA TTACCTATGA CGGATTAGAG
    GAAGATGATG AGGTCTTTGA AGTAATTCTG AACTCCCCTG TGAATGCAGT TCTTGGCACA
    AAGACAAAAG CTGCAGTGAA AATTTTGGAC TCAAAAGGAG GACAATGCCA TCCTTCATAT
    TCCTCCAACC AAAGCAAGCA CAGCACATGG GAGAAGGGCA TTTGGCATCT GCTGCCCCCA
    GGGTCTTCCT CATCCACCAC TTCTGGTTCC TTTCATCTGG AAAGAAGACC TCTTCCATCT
    TCCATGCAGC TAGCAGTCAC CAGGGGAGAC ACCCTGCGGG GCTTTGATTC TACAGATCTT
    TCTCAAAGGA AGCTTAGGAC CCGTGGGAAT GGCAAAACAG TTCGTCCATC CTCTGTTTAT
    AGAAATGGAA CAGACATCAT CTATAATTAT CATGGGATAG TTTCCTTGAA ACTAGAGGAT
    GACAGCTTCC CAACTCACAA AAGGAAGGCC AAAGTATCCA TCATTAGTCA GCCACAAAAG
    ACAATCAAAG TGGCAGAACT GCCTCAAGCA GATAAGGTGG AATCCACAAC TGACTCACAC
    TTCCCCAGAC AGGACCAGTT GCCCTCATTT CCAAAGAACT GCACTCTGGA ATTAAAGGGA
    CTCTTCCATT TTGAAGAAGG CATCCAGAAG CTGTATCAGT GCAATGGGAT TGCCTGGAAA
    GCCTGGAGTC CCCAAACCAA GGATGTGGAA GAAAAATCCT GTCCAGCCGG GTGGCACCAG
    CACTCAGGCT ACTGTCACAT CTTGATCACA GAGCAGAAAG GCACCTGGAA TGCGGCTGCC
    CAAGCTTGCA GGGAACAATA CCTGGGCAAC CTTGTAACTG TATTCTCCAG GCAGCACATG
    CGGTGGCTCT GGGACATTGG TGAGAGAAAG TCCTTTTGGA TAGGTTTGAA CGACCAAGTG
    CATGCTGGCC ACTGGGAGTG GATCGGTGGT GAACCTGTTG CCTTCACCAA TGGGAGAAGA
    GGGCCCTCTC AACGCTCCAA GCTTGGAAAG AGCTGTGTTT TGGTTCAAAG ACAAGGGAAA
    TGGCAAACAA AAGATTGTAG GAGAGCCAAA CCTCATAATT ATGTGTGTTC CAGAAAACTC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016816119.2
    CDS149..5791
    Translation

    Target ORF information:

    RefSeq Version XM_016960630.2
    Organism Pan troglodytes(chimpanzee)
    Definition Pan troglodytes FRAS1 related extracellular matrix 1 (FREM1), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_016960630.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGGCCATGT TTATTCTGGA AGTGGATCAG TTCATCCTGA CCTCCTTGAC TACATCAGTT 
    CTGGACTGTG AAGAAGATGA GACCCCTAAA CCCTTGCTGG TGTTCAACAT TACTAAAGCC
    CCGCTCCAGG GCTATGTGAC TCACCTGTTG GATCACACCA GACCAATCTC CTCATTCACC
    TGGAAAGATC TCAGTGACAT GCAGATCGCC TATCAGCCAC CAAACAGCAG CCATTCTGAG
    AGGAGACATG ATGAGGTAGA ATTGGAGGTA TACGACTTCT TCTTTGAAAG GAGTGCACCT
    ATGACAGTCC ACATCTCCAT CAGAACAGCA GATACAAATG CCCCCCGTGT ATCCTGGAAT
    ACAGGTCTGA ATCTCCTTGA GGGGCAGTCT CGAGCCATCA CTTGGGAACA GTTTCAGGTT
    GTCGACAATG ACGACATCGG TGCTGTCCGG CTAGTCACCG TTGGTGGCCT GCAGCATGGA
    TGGCTGACTT TAAGAGGGGG GAAAGGATTT CTCTTCACCG TGGCTGACCT CCAGGCTGGA
    GTTGTTCGCT ATCATCATGA TGACAGCGAC TCCACCAAAG ACTTCGTGGT CTTCCGGATA
    TTTGATGGCC ATCACAGCAT CCGTCACAAA TTCCCCATCA ACGTCTTGCC CAAAGATGAT
    AGTCCCCCGT TCCTCATAAC CAATGTTGTC ATTGAACTGG AGGAGGGGCA GACCATCCTG
    ATCCAGGGAT CCATGCTGCG AGCTTCAGAT GTGGATGCCA GTGATGACTA CATCTTCTTC
    AATATCACAA AGCCTCCACA GGCTGGGGAG ATCATGAAGA AGCCAGGGCC AGGACTGATA
    GGCTATCCTG TCCCTGGCTT CCTTCAGAGG GATTTGTTTA ATGGAATCAT TTATTATCGT
    CATTTTGGTG GAGAAATCTT TGAAGATTCT TTTCAATTTG TCCTGTGGGA CAGCCATGAA
    CCTCCAAATC TTTCAGTGCC ACAGGTGGCA ACAATCCATG TAACTCCAGT GGATGACCAG
    CTTCCAAAAG AGGCTCCTGG AGTTTCTCGG CATTTGGTTG TCAAGGAAAC TGAGGTGGCC
    TATATAACTA AGAAACAGCT ACATTTTATA GATTCAGAAT CATATGACAG GGAGCTGGTC
    TACACGATAA CTACTCCTCC ATTTTTCTCC TTCAGCCACA GACACTTGGA TGCTGGGAAA
    TTATTTATGG TGGACAGCAT ACCAAAAGTA GTTAAGAATC CTACGGCCCT GGAGCTGAGG
    TCATTCACTC AGCATGCTGT GAACTATATG AAAGTGGCCT ACATGCCCCC CATGCAAGAC
    ATTGGTCCCC ATTGCAGAGA TGTCCAGTTC ACATTTTCTG TCAGTAACCA ACATGGCGGC
    ACTTTGCATG GGATCTGCTT TAACATTACA ATCCTCCCAG TGGACAATCA AGTTCCTGAG
    GCGTTCACCA ACCCTCTGAA AGTGACTGAG GGAGGTCAAA GCATCATCAG CACAGAGCAC
    ATTCTGATTT CTGATGCAGA TACCAAGCTG GACAATATTG ACCTCTCCCT GCGGGAATTG
    CCTCTGCACG GAAGGGTGGA GCTGAATGGA TTTCCTCTAA ATTCAGGGGG CACATTTTCT
    TGGGGCGATC TCCATACCTT AAAAGTTAGG TATCAACATG ATGGAACTGA AGTTCTTCAG
    GATGACCTAC TCTTGGAGGT CACCGATGGC ACAAATTCAG CAGAATTTGT TCTACATGTT
    GAGGTATTCC CTGTCAACGA TGAGCCACCA GTCTTAAAGG CTGACCTCAT GCCTGTCATG
    AATTGCTCAG AGGGAGGAGA GGTGGTCATC ACCTCTGAAT ACATTTTTGC TACTGATGTG
    GACAGCGATA ACTTGAAGTT GATGTTTGTG ATTGCTCGCG AACCTCAGCA TGGGGTGGTG
    AGGAGAGCTG GAGTCACAGT GGATCAGTTC TCTCAGAGAG ATGTTATCTC AGAGGCCGTG
    ACATACAAAC ACACAGGTGG CGAGATTGGC ATGATGCCCT GTTTTGACAC CATTACATTG
    GTGGTTTCGG ATGGAGAGGC TGGCCCTTTT GTGAATGGCT GTTGCTACAA TGGACCCAAT
    CCATCTGTTC CGCTTCATGC GTCCTTTCCA GTGTATGATC TCAACATCAC GGTATACCCA
    GTAGACAACC AGCCACCTTC CATTGCAATA GGTCCCATGT TTGTTGTAGA TGAGGGCTGC
    TCAACAGCCC TTACTGTTAA CCATTTGTCC GCCACTGACC CTGACACTGC AGCAGATGAC
    TTGGAATTTG TTTTGGTTTC TCCTCCTCAG TTTGGCTACC TCGAAAATAT ACTCCCTTCT
    GTGGGTTTTG AAAAAAGCAA TATTGGCATA AGTATAGATT CATTTCAGTG GAAAGACATG
    AACGCTTTTC ACATTAACTA TGTGCAGTCC AGGCATCTGA GGATAGAACC AACTGCCGAC
    CAGTTCACGG TGTACGTCAC AGATGGGAAG CAACACTCCT TGGAGATACC ATTTTCTATT
    ATAATCAACC CCACAAATGA TGAAGCTCCT GACTTTGTAG TGCAGAATAT TACTGTGTGC
    GAGGGTCAGA TGAAAGAGCT GGACTCTTCC ATCATCAGCG CTGTGGACCT GGACATTCCC
    CAGGATGCCC TGCTGTTCAG CATCACTCAA AAGCCACGCC ATGGCCTCCT CATCGATAGG
    GGGTTTAGCA AAGACTTCTC TGAGAATAAG CAGCCAGCCA ACCCTCACCA GAAACATGTA
    CCTGTTCACA GTTTTTCCAT GGAACTCCTC AAGACTGGAA TGAGGTTGAC GTACATGCAT
    GATGACTCAG AGAGCCTTGC TGATGATTTT ACAATCCGTT TGTCAGATGG GAAACATAAG
    ATACTTAAAA CCATTTCAAT AGAGGTCATC CCAGTTAATG ATGAAAAACC AATGCTGAGC
    AAGAAGGCTG AAATTGCAAT GAATATGGGT GAAACTCGTA TTATTTCCAG TGCTATTCTT
    TCAGCCATAG ATGAAGACTC ACCCAGGGAG AAGATTTACT ATGTATTTGA AAGGCTTCCC
    CAAAATGGGC AACTTCAGCT TAAGATAGGG AGGGACTGGG TTCCTCTCTC CCCTGGCATG
    AAATGCACTC AGGAGGAAGT GGATCTGAAC TTGCTGAGAT ACACACACAC CGGGGCAATG
    GATTCCCAGA ATCAAGATAG CTTCACCTTC TACCTTTGGG ATGGCAACAA CAGGTCCCCT
    GCTCTTGACT GTCAAATCAC CATCAAGGAC ATGGAAAAAG GTGATATTGT CATCCTTACA
    AAACCACTCG TGGTGTCTAA AGGTGATAGA GGTTTCTTAA CAACCACCAC TCTCCTGGCT
    GTGGACGGAA CAGACAAGCC TGAGGAACTG CTCTATGTCA TCACCTCCCC TCCACGATAT
    GGCCAGATCG AATATGTTCA CTATCCTGGA GTTCCCATTA CAAACTTCAG CCAAATGGAT
    ATAGCGGGGC AGACAGTTTG CTATGTACAC AAGAGCAAGG TGACTGTCTC CAGTGACAGA
    TTCAGATTCA TCATCAGCAA TGGACTGCGG ACCGAGCACG GGGTGTTTGA GATCACACTG
    GAGACTGTGG ACAGAGCCCT GCCTGTGGTA ACCAGGAACA AGGGGTTGAG ACTGGCCCAA
    GGGGCCGTGG GCCTGCTTTC CCCTGACCTC CTTCAGCTGA CCGACCCTGA TACACCTGTG
    GAGAACCTCA CCTTCCTCTT GGTTCAGCTT CCCCAGCATG GCCAGCTCTA CCTGTGGGGG
    ACAGGGCTAC TTCAACACAA TTTCACCCAG CAGGATGTGG ACAGCAGGAA TGTGGCCTAT
    CGGCACTCAG GAGGGGACTC CCAGACTGAC TGCTTTACTT TCATGGCCAC AGACGGGACA
    AACCAAGGCT TTATTGTGAA TGGGAGAGTA TGGGAAGAAC CTGTTTTATT CACCATTCAG
    GTAGACCAAT TGGACAAAAC AGCTCCTCGT ATCACACTCT TGCATTCCCC TTCTCAAGTG
    GGGCTCCTGA AAAATGGCTG CTACGGGATT TACATCACTT CCCGCGTGTT GAAGGCATCA
    GACCCTGACA CTGAGGACGA TCAGATCATC TTTAAAATTC TACAAGGCCC AAAACATGGA
    CATCTGGAGA ACACAACAAC AGGTGAATTT ATCCATGAGA AATTTAGCCA AAAGGACTTA
    AACAGTAAGA CTATTCTTTA CATCATAAAC CCATCTTTGG AAGTAAATTC AGATACCGTG
    GAATTTCAAA TCATGGACCC CACAGGGAAC TCGGCCACTC CTCAAATTTT GGAACTGAAG
    TGGTCTCATA TTGAATGGTC ACAGACCGAA TATGAAGTCT GTGAGAATGT GGGTGTGTTG
    CCCTTGGAAA TTATCAGAAG GGGATATTCC ATGGACTCGG CCTTTGTGGG TATAAAGGTC
    AACCAAGTGT CAGCTGCAGA TGGAAAAGAT TTCACCGTGA TTCCATCTAA ACTGATTCAG
    TTTGACCCAG GAATGTCAAC TAAGATGTGG AATATAGCAA TTACCTATGA CGGATTAGAG
    GAAGATGATG AGGTCTTTGA AGTAATTCTG AACTCCCCTG TGAATGCAGT TCTTGGCACA
    AAGACAAAAG CTGCAGTGAA AATTTTGGAC TCAAAAGGAG GACAATGCCA TCCTTCATAT
    TCCTCCAACC AAAGCAAGCA CAGCACATGG GAGAAGGGCA TTTGGCATCT GCTGCCCCCA
    GGGTCTTCCT CATCCACCAC TTCTGGTTCC TTTCATCTGG AAAGAAGACC TCTTCCATCT
    TCCATGCAGC TAGCAGTCAC CAGGGGAGAC ACCCTGCGGG GCTTTGATTC TACAGATCTT
    TCTCAAAGGA AGCTTAGGAC CCGTGGGAAT GGCAAAACAG TTCGTCCATC CTCTGTTTAT
    AGAAATGGAA CAGACATCAT CTATAATTAT CATGGGATAG TTTCCTTGAA ACTAGAGGAT
    GACAGCTTCC CAACTCACAA AAGGAAGGCC AAAGTATCCA TCATTAGTCA GCCACAAAAG
    ACAATCAAAG TGGCAGAACT GCCTCAAGCA GATAAGGTGG AATCCACAAC TGACTCACAC
    TTCCCCAGAC AGGACCAGTT GCCCTCATTT CCAAAGAACT GCACTCTGGA ATTAAAGGGA
    CTCTTCCATT TTGAAGAAGG CATCCAGAAG CTGTATCAGT GCAATGGGAT TGCCTGGAAA
    GCCTGGAGTC CCCAAACCAA GGATGTGGAA GAAAAATCCT GTCCAGCCGG GTGGCACCAG
    CACTCAGGCT ACTGTCACAT CTTGATCACA GAGCAGAAAG GCACCTGGAA TGCGGCTGCC
    CAAGCTTGCA GGGAACAATA CCTGGGCAAC CTTGTAACTG TATTCTCCAG GCAGCACATG
    CGGTGGCTCT GGGACATTGG TGAGAGAAAG TCCTTTTGGA TAGGTTTGAA CGACCAAGTG
    CATGCTGGCC ACTGGGAGTG GATCGGTGGT GAACCTGTTG CCTTCACCAA TGGGAGAAGA
    GGGCCCTCTC AACGCTCCAA GCTTGGAAAG AGCTGTGTTT TGGTTCAAAG ACAAGGGAAA
    TGGCAAACAA AAGATTGTAG GAGAGCCAAA CCTCATAATT ATGTGTGTTC CAGAAAACTC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OPc109246
    Clone ID Related Accession (Same CDS sequence) XM_024345573.1
    Accession Version XM_024345573.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5511bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-03-19
    Organism Pan troglodytes(chimpanzee)
    Product FRAS1-related extracellular matrix protein 1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036888.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGAACTCTC TGAGTTGGGG GGCTGCGAAT GCCGTGCTGC TGCTGCTCCT CCTGGCCTGG 
    GCCAGCCCCA CCTTCATCAG CATCAACCGT GGGGTGAGGG TGATGAAGGG CCACTCTGCC
    TTCCTGTCAG GAGATGACCT GAAGTTTGCC ATCCCTAAAG AGAAAGATGC CTGCAAAGTG
    GAAGTTGTGA TGAATGAGCC AATAACCCAG AGGGTTGGGA AACTCACTCC ACAGGTCTTT
    GACTGCCATT TCCTTCCCAA TGAAGTCAAG TATGTTCACA ATGGTTGTCC AATTCTTGAT
    GAAGACACAG TGAAGCTTAG ACTTTACAGA TTTACTGAAA GAGATACCTT CATAGAAACC
    TTTATCCTGT GGGTCTATCT CCTGGAACCA GACTGTAACA TCATCCATAT GAGTAACAAT
    GTGCTGGAGG TGCCTGAATT CAATGGCCTG TCCCAAGCGA TTGATAAAAA TCTGCTCAGA
    TTCGATTATG ATAGGATGGC TAGCCTGGAA TGTACGGTCA GCCTGGACAC TGCGAGAACT
    CGGCTGCCAG CCCATGGCCA GATGGTTCTC GGGGAGCCTC GACCAGAAGA ACCTCGTGGA
    GATCAGCCAC ACAGTTTTTT CCCAGAGTCC CGTATGATGT CTTTTAATGC CTTTCTGGAA
    CTGAGAGCAA AACTGAAGTG TCCAGGTGGA AGCTGTACCC CAGGATTAAA GAAAATAGGA
    AGCCTCAAAG TGAGCTGTGA GGAGTTCCTG CTGATGGGCC TTCGTTATCA GCATCTGGAT
    CCCCCTTCAC CCAACATTGA TTATATCTCC ATCCAACTGG ACCTCACAGA TACCAGGAGT
    AAAATTGTGT ACAAGTCAGA GAGTGCGTGG CTGCCTGTCT ATATCAGAGC TGGAATTCCG
    AATCAGATTC CAAAGGCTGC ATTCATGGCC ATGTTTATTC TGGAAGTGGA TCAGTTCATC
    CTGACCTCCT TGACTACATC AGTTCTGGAC TGTGAAGAAG ATGAGACCCC TAAACCCTTG
    CTGGTGTTCA ACATTACTAA AGCCCCGCTC CAGGGCTATG TGACTCACCT GTTGGATCAC
    ACCAGACCAA TCTCCTCATT CACCTGGAAA GATCTCAGTG ACATGCAGAT CGCCTATCAG
    CCACCAAACA GCAGCCATTC TGAGAGGAGA CATGATGAGG TAGAATTGGA GGTATACGAC
    TTCTTCTTTG AAAGGAGTGC ACCTATGACA GTCCACATCT CCATCAGAAC AGCAGATACA
    AATGCCCCCC GTGTATCCTG GAATACAGGT CTGAATCTCC TTGAGGGGCA GTCTCGAGCC
    ATCACTTGGG AACAGTTTCA GGTTGTCGAC AATGACGACA TCGGTGCTGT CCGGCTAGTC
    ACCGTTGGTG GCCTGCAGCA TGGATGGCTG ACTTTAAGAG GGGGGAAAGG ATTTCTCTTC
    ACCGTGGCTG ACCTCCAGGC TGGAGTTGTT CGCTATCATC ATGATGACAG CGACTCCACC
    AAAGACTTCG TGGTCTTCCG GATATTTGAT GGCCATCACA GCATCCGTCA CAAATTCCCC
    ATCAACGTCT TGCCCAAAGA TGATAGTCCC CCGTTCCTCA TAACCAATGT TGTCATTGAA
    CTGGAGGAGG GGCAGACCAT CCTGATCCAG GGATCCATGC TGCGAGCTTC AGATGTGGAT
    GCCAGTGATG ACTACATCTT CTTCAATATC ACAAAGCCTC CACAGGCTGG GGAGATCATG
    AAGAAGCCAG GGCCAGGACT GATAGGCTAT CCTGTCCCTG GCTTCCTTCA GAGGGATTTG
    TTTAATGGAA TCATTTATTA TCGTCATTTT GGTGGAGAAA TCTTTGAAGA TTCTTTTCAA
    TTTGTCCTGT GGGACAGCCA TGAACCTCCA AATCTTTCAG TGCCACAGGT GGCAACAATC
    CATGTAACTC CAGTGGATGA CCAGCTTCCA AAAGAGGCTC CTGGAGTTTC TCGGCATTTG
    GTTGTCAAGG AAACTGAGGT GGCCTATATA ACTAAGAAAC AGCTACATTT TATAGATTCA
    GAATCATATG ACAGGGAGCT GGTCTACACG ATAACTACTC CTCCATTTTT CTCCTTCAGC
    CACAGACACT TGGATGCTGG GAAATTATTT ATGGTGGACA GCATACCAAA AGTAGTTAAG
    AATCCTACGG CCCTGGAGCT GAGGTCATTC ACTCAGCATG CTGTGAACTA TATGAAAGTG
    GCCTACATGC CCCCCATGCA AGACATTGGT CCCCATTGCA GAGATGTCCA GTTCACATTT
    TCTGTCAGTA ACCAACATGG CGGCACTTTG CATGGGATCT GCTTTAACAT TACAATCCTC
    CCAGTGGACA ATCAAGTTCC TGAGGCGTTC ACCAACCCTC TGAAAGTGAC TGAGGGAGGT
    CAAAGCATCA TCAGCACAGA GCACATTCTG ATTTCTGATG CAGATACCAA GCTGGACAAT
    ATTGACCTCT CCCTGCGGGA ATTGCCTCTG CACGGAAGGG TGGAGCTGAA TGGATTTCCT
    CTAAATTCAG GGGGCACATT TTCTTGGGGC GATCTCCATA CCTTAAAAGT TAGGTATCAA
    CATGATGGAA CTGAAGTTCT TCAGGATGAC CTACTCTTGG AGGTCACCGA TGGCACAAAT
    TCAGCAGAAT TTGTTCTACA TGTTGAGGTA TTCCCTGTCA ACGATGAGCC ACCAGTCTTA
    AAGGCTGACC TCATGCCTGT CATGAATTGC TCAGAGGGAG GAGAGGTGGT CATCACCTCT
    GAATACATTT TTGCTACTGA TGTGGACAGC GATAACTTGA AGTTGATGTT TGTGATTGCT
    CGCGAACCTC AGCATGGGGT GGTGAGGAGA GCTGGAGTCA CAGTGGATCA GTTCTCTCAG
    AGAGATGTTA TCTCAGAGGC CGTGACATAC AAACACACAG GTGGCGAGAT TGGCATGATG
    CCCTGTTTTG ACACCATTAC ATTGGTGGTT TCGGATGGAG AGGCTGGCCC TTTTGTGAAT
    GGCTGTTGCT ACAATGGACC CAATCCATCT GTTCCGCTTC ATGCGTCCTT TCCAGTGTAT
    GATCTCAACA TCACGGTATA CCCAGTAGAC AACCAGCCAC CTTCCATTGC AATAGGTCCC
    ATGTTTGTTG TAGATGAGGG CTGCTCAACA GCCCTTACTG TTAACCATTT GTCCGCCACT
    GACCCTGACA CTGCAGCAGA TGACTTGGAA TTTGTTTTGG TTTCTCCTCC TCAGTTTGGC
    TACCTCGAAA ATATACTCCC TTCTGTGGGT TTTGAAAAAA GCAATATTGG CATAAGTATA
    GATTCATTTC AGTGGAAAGA CATGAACGCT TTTCACATTA ACTATGTGCA GTCCAGGCAT
    CTGAGGATAG AACCAACTGC CGACCAGTTC ACGGTGTACG TCACAGATGG GAAGCAACAC
    TCCTTGGAGA TACCATTTTC TATTATAATC AACCCCACAA ATGATGAAGC TCCTGACTTT
    GTAGTGCAGA ATATTACTGT GTGCGAGGGT CAGATGAAAG AGCTGGACTC TTCCATCATC
    AGCGCTGTGG ACCTGGACAT TCCCCAGGAT GCCCTGCTGT TCAGCATCAC TCAAAAGCCA
    CGCCATGGCC TCCTCATCGA TAGGGGGTTT AGCAAAGACT TCTCTGAGAA TAAGCAGCCA
    GCCAACCCTC ACCAGAAACA TGTACCTGTT CACAGTTTTT CCATGGAACT CCTCAAGACT
    GGAATGAGGT TGACGTACAT GCATGATGAC TCAGAGAGCC TTGCTGATGA TTTTACAATC
    CGTTTGTCAG ATGGGAAACA TAAGATACTT AAAACCATTT CAATAGAGGT CATCCCAGTT
    AATGATGAAA AACCAATGCT GAGCAAGAAG GCTGAAATTG CAATGAATAT GGGTGAAACT
    CGTATTATTT CCAGTGCTAT TCTTTCAGCC ATAGATGAAG ACTCACCCAG GGAGAAGATT
    TACTATGTAT TTGAAAGGCT TCCCCAAAAT GGGCAACTTC AGCTTAAGAT AGGGAGGGAC
    TGGGTTCCTC TCTCCCCTGG CATGAAATGC ACTCAGGAGG AAGTGGATCT GAACTTGCTG
    AGATACACAC ACACCGGGGC AATGGATTCC CAGAATCAAG ATAGCTTCAC CTTCTACCTT
    TGGGATGGCA ACAACAGGTC CCCTGCTCTT GACTGTCAAA TCACCATCAA GGACATGGAA
    AAAGGTGATA TTGTCATCCT TACAAAACCA CTCGTGGTGT CTAAAGGTGA TAGAGGTTTC
    TTAACAACCA CCACTCTCCT GGCTGTGGAC GGAACAGACA AGCCTGAGGA ACTGCTCTAT
    GTCATCACCT CCCCTCCACG ATATGGCCAG ATCGAATATG TTCACTATCC TGGAGTTCCC
    ATTACAAACT TCAGCCAAAT GGATATAGCG GGGCAGACAG TTTGCTATGT ACACAAGAGC
    AAGGTGACTG TCTCCAGTGA CAGATTCAGA TTCATCATCA GCAATGGACT GCGGACCGAG
    CACGGGGTGT TTGAGATCAC ACTGGAGACT GTGGACAGAG CCCTGCCTGT GGTAACCAGG
    AACAAGGGGT TGAGACTGGC CCAAGGGGCC GTGGGCCTGC TTTCCCCTGA CCTCCTTCAG
    CTGACCGACC CTGATACACC TGTGGAGAAC CTCACCTTCC TCTTGGTTCA GCTTCCCCAG
    CATGGCCAGC TCTACCTGTG GGGGACAGGG CTACTTCAAC ACAATTTCAC CCAGCAGGAT
    GTGGACAGCA GGAATGTGGC CTATCGGCAC TCAGGAGGGG ACTCCCAGAC TGACTGCTTT
    ACTTTCATGG CCACAGACGG GACAAACCAA GGCTTTATTG TGAATGGGAG AGTATGGGAA
    GAACCTGTTT TATTCACCAT TCAGGTAGAC CAATTGGACA AAACAGCTCC TCGTATCACA
    CTCTTGCATT CCCCTTCTCA AGTGGGGCTC CTGAAAAATG GCTGCTACGG GATTTACATC
    ACTTCCCGCG TGTTGAAGGC ATCAGACCCT GACACTGAGG ACGATCAGAT CATCTTTAAA
    ATTCTACAAG GCCCAAAACA TGGACATCTG GAGAACACAA CAACAGGTGA ATTTATCCAT
    GAGAAATTTA GCCAAAAGGA CTTAAACAGT AAGACTATTC TTTACATCAT AAACCCATCT
    TTGGAAGTAA ATTCAGATAC CGTGGAATTT CAAATCATGG ACCCCACAGG GAACTCGGCC
    ACTCCTCAAA TTTTGGAACT GAAGTGGTCT CATATTGAAT GGTCACAGAC CGAATATGAA
    GTCTGTGAGA ATGTGGGTGT GTTGCCCTTG GAAATTATCA GAAGGGGATA TTCCATGGAC
    TCGGCCTTTG TGGGTATAAA GGTCAACCAA GTGTCAGCTG CAGATGGAAA AGATTTCACC
    GTGATTCCAT CTAAACTGAT TCAGTTTGAC CCAGGTGCAA GTGGAAATAA AAAAGGCTGG
    TACTTGCTCT CAAGTGTGTT CCATTGTGTG AGAAACACAG ACAAGAATTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024201341.1
    CDS2125..7635
    Translation

    Target ORF information:

    RefSeq Version XM_024345573.1
    Organism Pan troglodytes(chimpanzee)
    Definition Pan troglodytes FRAS1 related extracellular matrix 1 (FREM1), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024345573.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGAACTCTC TGAGTTGGGG GGCTGCGAAT GCCGTGCTGC TGCTGCTCCT CCTGGCCTGG 
    GCCAGCCCCA CCTTCATCAG CATCAACCGT GGGGTGAGGG TGATGAAGGG CCACTCTGCC
    TTCCTGTCAG GAGATGACCT GAAGTTTGCC ATCCCTAAAG AGAAAGATGC CTGCAAAGTG
    GAAGTTGTGA TGAATGAGCC AATAACCCAG AGGGTTGGGA AACTCACTCC ACAGGTCTTT
    GACTGCCATT TCCTTCCCAA TGAAGTCAAG TATGTTCACA ATGGTTGTCC AATTCTTGAT
    GAAGACACAG TGAAGCTTAG ACTTTACAGA TTTACTGAAA GAGATACCTT CATAGAAACC
    TTTATCCTGT GGGTCTATCT CCTGGAACCA GACTGTAACA TCATCCATAT GAGTAACAAT
    GTGCTGGAGG TGCCTGAATT CAATGGCCTG TCCCAAGCGA TTGATAAAAA TCTGCTCAGA
    TTCGATTATG ATAGGATGGC TAGCCTGGAA TGTACGGTCA GCCTGGACAC TGCGAGAACT
    CGGCTGCCAG CCCATGGCCA GATGGTTCTC GGGGAGCCTC GACCAGAAGA ACCTCGTGGA
    GATCAGCCAC ACAGTTTTTT CCCAGAGTCC CGTATGATGT CTTTTAATGC CTTTCTGGAA
    CTGAGAGCAA AACTGAAGTG TCCAGGTGGA AGCTGTACCC CAGGATTAAA GAAAATAGGA
    AGCCTCAAAG TGAGCTGTGA GGAGTTCCTG CTGATGGGCC TTCGTTATCA GCATCTGGAT
    CCCCCTTCAC CCAACATTGA TTATATCTCC ATCCAACTGG ACCTCACAGA TACCAGGAGT
    AAAATTGTGT ACAAGTCAGA GAGTGCGTGG CTGCCTGTCT ATATCAGAGC TGGAATTCCG
    AATCAGATTC CAAAGGCTGC ATTCATGGCC ATGTTTATTC TGGAAGTGGA TCAGTTCATC
    CTGACCTCCT TGACTACATC AGTTCTGGAC TGTGAAGAAG ATGAGACCCC TAAACCCTTG
    CTGGTGTTCA ACATTACTAA AGCCCCGCTC CAGGGCTATG TGACTCACCT GTTGGATCAC
    ACCAGACCAA TCTCCTCATT CACCTGGAAA GATCTCAGTG ACATGCAGAT CGCCTATCAG
    CCACCAAACA GCAGCCATTC TGAGAGGAGA CATGATGAGG TAGAATTGGA GGTATACGAC
    TTCTTCTTTG AAAGGAGTGC ACCTATGACA GTCCACATCT CCATCAGAAC AGCAGATACA
    AATGCCCCCC GTGTATCCTG GAATACAGGT CTGAATCTCC TTGAGGGGCA GTCTCGAGCC
    ATCACTTGGG AACAGTTTCA GGTTGTCGAC AATGACGACA TCGGTGCTGT CCGGCTAGTC
    ACCGTTGGTG GCCTGCAGCA TGGATGGCTG ACTTTAAGAG GGGGGAAAGG ATTTCTCTTC
    ACCGTGGCTG ACCTCCAGGC TGGAGTTGTT CGCTATCATC ATGATGACAG CGACTCCACC
    AAAGACTTCG TGGTCTTCCG GATATTTGAT GGCCATCACA GCATCCGTCA CAAATTCCCC
    ATCAACGTCT TGCCCAAAGA TGATAGTCCC CCGTTCCTCA TAACCAATGT TGTCATTGAA
    CTGGAGGAGG GGCAGACCAT CCTGATCCAG GGATCCATGC TGCGAGCTTC AGATGTGGAT
    GCCAGTGATG ACTACATCTT CTTCAATATC ACAAAGCCTC CACAGGCTGG GGAGATCATG
    AAGAAGCCAG GGCCAGGACT GATAGGCTAT CCTGTCCCTG GCTTCCTTCA GAGGGATTTG
    TTTAATGGAA TCATTTATTA TCGTCATTTT GGTGGAGAAA TCTTTGAAGA TTCTTTTCAA
    TTTGTCCTGT GGGACAGCCA TGAACCTCCA AATCTTTCAG TGCCACAGGT GGCAACAATC
    CATGTAACTC CAGTGGATGA CCAGCTTCCA AAAGAGGCTC CTGGAGTTTC TCGGCATTTG
    GTTGTCAAGG AAACTGAGGT GGCCTATATA ACTAAGAAAC AGCTACATTT TATAGATTCA
    GAATCATATG ACAGGGAGCT GGTCTACACG ATAACTACTC CTCCATTTTT CTCCTTCAGC
    CACAGACACT TGGATGCTGG GAAATTATTT ATGGTGGACA GCATACCAAA AGTAGTTAAG
    AATCCTACGG CCCTGGAGCT GAGGTCATTC ACTCAGCATG CTGTGAACTA TATGAAAGTG
    GCCTACATGC CCCCCATGCA AGACATTGGT CCCCATTGCA GAGATGTCCA GTTCACATTT
    TCTGTCAGTA ACCAACATGG CGGCACTTTG CATGGGATCT GCTTTAACAT TACAATCCTC
    CCAGTGGACA ATCAAGTTCC TGAGGCGTTC ACCAACCCTC TGAAAGTGAC TGAGGGAGGT
    CAAAGCATCA TCAGCACAGA GCACATTCTG ATTTCTGATG CAGATACCAA GCTGGACAAT
    ATTGACCTCT CCCTGCGGGA ATTGCCTCTG CACGGAAGGG TGGAGCTGAA TGGATTTCCT
    CTAAATTCAG GGGGCACATT TTCTTGGGGC GATCTCCATA CCTTAAAAGT TAGGTATCAA
    CATGATGGAA CTGAAGTTCT TCAGGATGAC CTACTCTTGG AGGTCACCGA TGGCACAAAT
    TCAGCAGAAT TTGTTCTACA TGTTGAGGTA TTCCCTGTCA ACGATGAGCC ACCAGTCTTA
    AAGGCTGACC TCATGCCTGT CATGAATTGC TCAGAGGGAG GAGAGGTGGT CATCACCTCT
    GAATACATTT TTGCTACTGA TGTGGACAGC GATAACTTGA AGTTGATGTT TGTGATTGCT
    CGCGAACCTC AGCATGGGGT GGTGAGGAGA GCTGGAGTCA CAGTGGATCA GTTCTCTCAG
    AGAGATGTTA TCTCAGAGGC CGTGACATAC AAACACACAG GTGGCGAGAT TGGCATGATG
    CCCTGTTTTG ACACCATTAC ATTGGTGGTT TCGGATGGAG AGGCTGGCCC TTTTGTGAAT
    GGCTGTTGCT ACAATGGACC CAATCCATCT GTTCCGCTTC ATGCGTCCTT TCCAGTGTAT
    GATCTCAACA TCACGGTATA CCCAGTAGAC AACCAGCCAC CTTCCATTGC AATAGGTCCC
    ATGTTTGTTG TAGATGAGGG CTGCTCAACA GCCCTTACTG TTAACCATTT GTCCGCCACT
    GACCCTGACA CTGCAGCAGA TGACTTGGAA TTTGTTTTGG TTTCTCCTCC TCAGTTTGGC
    TACCTCGAAA ATATACTCCC TTCTGTGGGT TTTGAAAAAA GCAATATTGG CATAAGTATA
    GATTCATTTC AGTGGAAAGA CATGAACGCT TTTCACATTA ACTATGTGCA GTCCAGGCAT
    CTGAGGATAG AACCAACTGC CGACCAGTTC ACGGTGTACG TCACAGATGG GAAGCAACAC
    TCCTTGGAGA TACCATTTTC TATTATAATC AACCCCACAA ATGATGAAGC TCCTGACTTT
    GTAGTGCAGA ATATTACTGT GTGCGAGGGT CAGATGAAAG AGCTGGACTC TTCCATCATC
    AGCGCTGTGG ACCTGGACAT TCCCCAGGAT GCCCTGCTGT TCAGCATCAC TCAAAAGCCA
    CGCCATGGCC TCCTCATCGA TAGGGGGTTT AGCAAAGACT TCTCTGAGAA TAAGCAGCCA
    GCCAACCCTC ACCAGAAACA TGTACCTGTT CACAGTTTTT CCATGGAACT CCTCAAGACT
    GGAATGAGGT TGACGTACAT GCATGATGAC TCAGAGAGCC TTGCTGATGA TTTTACAATC
    CGTTTGTCAG ATGGGAAACA TAAGATACTT AAAACCATTT CAATAGAGGT CATCCCAGTT
    AATGATGAAA AACCAATGCT GAGCAAGAAG GCTGAAATTG CAATGAATAT GGGTGAAACT
    CGTATTATTT CCAGTGCTAT TCTTTCAGCC ATAGATGAAG ACTCACCCAG GGAGAAGATT
    TACTATGTAT TTGAAAGGCT TCCCCAAAAT GGGCAACTTC AGCTTAAGAT AGGGAGGGAC
    TGGGTTCCTC TCTCCCCTGG CATGAAATGC ACTCAGGAGG AAGTGGATCT GAACTTGCTG
    AGATACACAC ACACCGGGGC AATGGATTCC CAGAATCAAG ATAGCTTCAC CTTCTACCTT
    TGGGATGGCA ACAACAGGTC CCCTGCTCTT GACTGTCAAA TCACCATCAA GGACATGGAA
    AAAGGTGATA TTGTCATCCT TACAAAACCA CTCGTGGTGT CTAAAGGTGA TAGAGGTTTC
    TTAACAACCA CCACTCTCCT GGCTGTGGAC GGAACAGACA AGCCTGAGGA ACTGCTCTAT
    GTCATCACCT CCCCTCCACG ATATGGCCAG ATCGAATATG TTCACTATCC TGGAGTTCCC
    ATTACAAACT TCAGCCAAAT GGATATAGCG GGGCAGACAG TTTGCTATGT ACACAAGAGC
    AAGGTGACTG TCTCCAGTGA CAGATTCAGA TTCATCATCA GCAATGGACT GCGGACCGAG
    CACGGGGTGT TTGAGATCAC ACTGGAGACT GTGGACAGAG CCCTGCCTGT GGTAACCAGG
    AACAAGGGGT TGAGACTGGC CCAAGGGGCC GTGGGCCTGC TTTCCCCTGA CCTCCTTCAG
    CTGACCGACC CTGATACACC TGTGGAGAAC CTCACCTTCC TCTTGGTTCA GCTTCCCCAG
    CATGGCCAGC TCTACCTGTG GGGGACAGGG CTACTTCAAC ACAATTTCAC CCAGCAGGAT
    GTGGACAGCA GGAATGTGGC CTATCGGCAC TCAGGAGGGG ACTCCCAGAC TGACTGCTTT
    ACTTTCATGG CCACAGACGG GACAAACCAA GGCTTTATTG TGAATGGGAG AGTATGGGAA
    GAACCTGTTT TATTCACCAT TCAGGTAGAC CAATTGGACA AAACAGCTCC TCGTATCACA
    CTCTTGCATT CCCCTTCTCA AGTGGGGCTC CTGAAAAATG GCTGCTACGG GATTTACATC
    ACTTCCCGCG TGTTGAAGGC ATCAGACCCT GACACTGAGG ACGATCAGAT CATCTTTAAA
    ATTCTACAAG GCCCAAAACA TGGACATCTG GAGAACACAA CAACAGGTGA ATTTATCCAT
    GAGAAATTTA GCCAAAAGGA CTTAAACAGT AAGACTATTC TTTACATCAT AAACCCATCT
    TTGGAAGTAA ATTCAGATAC CGTGGAATTT CAAATCATGG ACCCCACAGG GAACTCGGCC
    ACTCCTCAAA TTTTGGAACT GAAGTGGTCT CATATTGAAT GGTCACAGAC CGAATATGAA
    GTCTGTGAGA ATGTGGGTGT GTTGCCCTTG GAAATTATCA GAAGGGGATA TTCCATGGAC
    TCGGCCTTTG TGGGTATAAA GGTCAACCAA GTGTCAGCTG CAGATGGAAA AGATTTCACC
    GTGATTCCAT CTAAACTGAT TCAGTTTGAC CCAGGTGCAA GTGGAAATAA AAAAGGCTGG
    TACTTGCTCT CAAGTGTGTT CCATTGTGTG AGAAACACAG ACAAGAATTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OPc88759
    Clone ID Related Accession (Same CDS sequence) XM_016960630.1
    Accession Version XM_016960630.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5643bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-01
    Organism Pan troglodytes(chimpanzee)
    Product FRAS1-related extracellular matrix protein 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006476.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGGCCATGT TTATTCTGGA AGTGGATCAG TTCATCCTGA CCTCCTTGAC TACATCAGTT 
    CTGGACTGTG AAGAAGATGA GACCCCTAAA CCCTTGCTGG TGTTCAACAT TACTAAAGCC
    CCGCTCCAGG GCTATGTGAC TCACCTGTTG GATCACACCA GACCAATCTC CTCATTCACC
    TGGAAAGATC TCAGTGACAT GCAGATCGCC TATCAGCCAC CAAACAGCAG CCATTCTGAG
    AGGAGACATG ATGAGGTAGA ATTGGAGGTA TACGACTTCT TCTTTGAAAG GAGTGCACCT
    ATGACAGTCC ACATCTCCAT CAGAACAGCA GATACAAATG CCCCCCGTGT ATCCTGGAAT
    ACAGGTCTGA ATCTCCTTGA GGGGCAGTCT CGAGCCATCA CTTGGGAACA GTTTCAGGTT
    GTCGACAATG ACGACATCGG TGCTGTCCGG CTAGTCACCG TTGGTGGCCT GCAGCATGGA
    TGGCTGACTT TAAGAGGGGG GAAAGGATTT CTCTTCACCG TGGCTGACCT CCAGGCTGGA
    GTTGTTCGCT ATCATCATGA TGACAGCGAC TCCACCAAAG ACTTCGTGGT CTTCCGGATA
    TTTGATGGCC ATCACAGCAT CCGTCACAAA TTCCCCATCA ACGTCTTGCC CAAAGATGAT
    AGTCCCCCGT TCCTCATAAC CAATGTTGTC ATTGAACTGG AGGAGGGGCA GACCATCCTG
    ATCCAGGGAT CCATGCTGCG AGCTTCAGAT GTGGATGCCA GTGATGACTA CATCTTCTTC
    AATATCACAA AGCCTCCACA GGCTGGGGAG ATCATGAAGA AGCCAGGGCC AGGACTGATA
    GGCTATCCTG TCCCTGGCTT CCTTCAGAGG GATTTGTTTA ATGGAATCAT TTATTATCGT
    CATTTTGGTG GAGAAATCTT TGAAGATTCT TTTCAATTTG TCCTGTGGGA CAGCCATGAA
    CCTCCAAATC TTTCAGTGCC ACAGGTGGCA ACAATCCATG TAACTCCAGT GGATGACCAG
    CTTCCAAAAG AGGCTCCTGG AGTTTCTCGG CATTTGGTTG TCAAGGAAAC TGAGGTGGCC
    TATATAACTA AGAAACAGCT ACATTTTATA GATTCAGAAT CATATGACAG GGAGCTGGTC
    TACACGATAA CTACTCCTCC ATTTTTCTCC TTCAGCCACA GACACTTGGA TGCTGGGAAA
    TTATTTATGG TGGACAGCAT ACCAAAAGTA GTTAAGAATC CTACGGCCCT GGAGCTGAGG
    TCATTCACTC AGCATGCTGT GAACTATATG AAAGTGGCCT ACATGCCCCC CATGCAAGAC
    ATTGGTCCCC ATTGCAGAGA TGTCCAGTTC ACATTTTCTG TCAGTAACCA ACATGGCGGC
    ACTTTGCATG GGATCTGCTT TAACATTACA ATCCTCCCAG TGGACAATCA AGTTCCTGAG
    GCGTTCACCA ACCCTCTGAA AGTGACTGAG GGAGGTCAAA GCATCATCAG CACAGAGCAC
    ATTCTGATTT CTGATGCAGA TACCAAGCTG GACAATATTG ACCTCTCCCT GCGGGAATTG
    CCTCTGCACG GAAGGGTGGA GCTGAATGGA TTTCCTCTAA ATTCAGGGGG CACATTTTCT
    TGGGGCGATC TCCATACCTT AAAAGTTAGG TATCAACATG ATGGAACTGA AGTTCTTCAG
    GATGACCTAC TCTTGGAGGT CACCGATGGC ACAAATTCAG CAGAATTTGT TCTACATGTT
    GAGGTATTCC CTGTCAACGA TGAGCCACCA GTCTTAAAGG CTGACCTCAT GCCTGTCATG
    AATTGCTCAG AGGGAGGAGA GGTGGTCATC ACCTCTGAAT ACATTTTTGC TACTGATGTG
    GACAGCGATA ACTTGAAGTT GATGTTTGTG ATTGCTCGCG AACCTCAGCA TGGGGTGGTG
    AGGAGAGCTG GAGTCACAGT GGATCAGTTC TCTCAGAGAG ATGTTATCTC AGAGGCCGTG
    ACATACAAAC ACACAGGTGG CGAGATTGGC ATGATGCCCT GTTTTGACAC CATTACATTG
    GTGGTTTCGG ATGGAGAGGC TGGCCCTTTT GTGAATGGCT GTTGCTACAA TGGACCCAAT
    CCATCTGTTC CGCTTCATGC GTCCTTTCCA GTGTATGATC TCAACATCAC GGTATACCCA
    GTAGACAACC AGCCACCTTC CGTTGCAATA GGTCCCATGT TTGTTGTAGA TGAGGGCTGC
    TCAACAGCCC TTACTGTTAA CCATTTGTCC GCCACTGACC CTGACACTGC AGCAGATGAC
    TTGGAATTTG TTTTGGTTTC TCCTCCTCAG TTTGGCTACC TCGAAAATAT ACTCCCTTCT
    GTGGGTTTTG AAAAAAGCAA TATTGGCATA AGTATAGATT CATTTCAGTG GAAAGACATG
    AACGCTTTTC ACATTAACTA TGTGCAGTCC AGGCATCTGA GGATAGAACC AACTGCCGAC
    CAGTTCACGG TGTACGTCAC AGATGGGAAG CAACACTCCT TGGAGATACC ATTTTCTATT
    ATAATCAACC CCACAAATGA TGAAGCTCCT GACTTTGTAG TGCAGAATAT TACTGTGTGC
    GAGGGTCAGA TGAAAGAGCT GGACTCTTCC ATCATCAGCG CTGTGGACCT GGACATTCCC
    CAGGATGCCC TGCTGTTCAG CATCACTCAA AAGCCACGCC ATGGCCTCCT CATCGATAGG
    GGGTTTAGCA AAGACTTCTC TGAGAATAAG CAGCCAGCCA ACCCTCACCA GAAACATGTA
    CCTGTTCACA GTTTTTCCAT GGAACTCCTC AAGACTGGAA TGAGGTTGAC GTACATGCAT
    GATGACTCAG AGAGCCTTGC TGATGATTTT ACAATCCGTT TGTCAGATGG GAAACATAAG
    ATACTTAAAA CCATTTCAAT AGAGGTCATC CCAGTTAATG ATGAAAAACC AATGCTGAGC
    AAGAAGGCTG AAATTGCAAT GAATATGGGT GAAACTCGTA TTATTTCCAG TGCTATTCTT
    TCAGCCATAG ATGAAGACTC ACCCAGGGAG AAGATTTACT ATGTATTTGA AAGGCTTCCC
    CAAAATGGGC AACTTCAGCT TAAGATAGGG AGGGACTGGG TTCCTCTCTC CCCTGGCATG
    AAATGCACTC AGGAGGAAGT GGATCTGAAC TTGCTGAGAT ACACACACAC CGGGGCAATG
    GATTCCCAGA ATCAAGATAG CTTCACCTTC TACCTTTGGG ATGGCAACAA CAGGTCCCCT
    GCTCTTGACT GTCAAATCAC CATCAAGGAC ATGGAAAAAG GTGATATTGT CATCCTTACA
    AAACCACTCG TGGTGTCTAA AGGTGATAGA GGTTTCTTAA CAACCACCAC TCTCCTGGCT
    GTGGACGGAA CAGACAAGCC TGAGGAACTG CTCTATGTCA TCACCTCCCC TCCACGATAT
    GGCCAGATCG AATATGTTCA CTATCCTGGA GTTCCCATTA CAAACTTCAG CCAAATGGAT
    ATAGCGGGGC AGACAGTTTG CTATGTACAC AAGAGCAAGG TGACTGTCTC CAGTGACAGA
    TTCAGATTCA TCATCAGCAA TGGACTGCGG ACCGAGCACG GGGTGTTTGA GATCACACTG
    GAGACTGTGG ACAGAGCCCT GCCTGTGGTA ACCAGGAACA AGGGGTTGAG ACTGGCCCAA
    GGGGCCGTGG GCCTGCTTTC CCCTGACCTC CTTCAGCTGA CCGACCCTGA TACACCTGTG
    GAGAACCTCA CCTTCCTCTT GGTTCAGCTT CCCCAGCATG GCCAGCTCTA CCTGTGGGGG
    ACAGGGCTAC TTCAACACAA TTTCACCCAG CAGGATGTGG ACAGCAGGAA TGTGGCCTAT
    CGGCACTCAG GAGGGGACTC CCAGACTGAC TGCTTTACTT TCATGGCCAC AGACGGGACA
    AACCAAGGCT TTATTGTGAA TGGGAGAGTA TGGGAAGAAC CTGTTTTATT CACCATTCAG
    GTAGACCAAT TGGACAAAAC AGCTCCTCGT ATCACACTCT TGCATTCCCC TTCTCAAGTG
    GGGCTCCTGA AAAATGGCTG CTACGGGATT TACATCACTT CCCGCGTGTT GAAGGCATCA
    GACCCTGACA CTGAGGACGA TCAGATCATC TTTAAAATTC TACAAGGCCC AAAACATGGA
    CATCTGGAGA ACACAACAAC AGGTGAATTT ATCCATGAGA AATTTAGCCA AAAGGACTTA
    AACAGTAAGA CTATTCTTTA CATCATAAAC CCATCTTTGG AAGTAAATTC AGATACCGTG
    GAATTTCAAA TCATGGACCC CACAGGGAAC TCGGCCACTC CTCAAATTTT GGAACTGAAG
    TGGTCTCATA TTGAATGGTC ACAGACCGAA TATGAAGTCT GTGAGAATGT GGGTGTGTTG
    CCCTTGGAAA TTATCAGAAG GGGATATTCC ATGGACTCGG CCTTTGTGGG TATAAAGGTC
    AACCAAGTGT CAGCTGCAGA TGGAAAAGAT TTCACCGTGA TTCCATCTAA ACTGATTCAG
    TTTGACCCAG GAATGTCAAC TAAGATGTGG AATATAGCAA TTACCTATGA CGGATTAGAG
    GAAGATGATG AGGTCTTTGA AGTAATTCTG AACTCCCCTG TGAATGCAGT TCTTGGCACA
    AAGACAAAAG CTGCAGTGAA AATTTTGGAC TCAAAAGGAG GACAATGCCA TCCTTCATAT
    TCCTCCAACC AAAGCAAGCA CAGCACATGG GAGAAGGGCA TTTGGCATCT GCTGCCCCCA
    GGGTCTTCCT CATCCACCAC TTCTGGTTCC TTTCATCTGG AAAGAAGACC TCTTCCATCT
    TCCATGCAGC TAGCAGTCAC CAGGGGAGAC ACCCTGCGGG GCTTTGATTC TACAGATCTT
    TCTCAAAGGA AGCTTAGGAC CCGTGGGAAT GGCAAAACAG TTCGTCCATC CTCTGTTTAT
    AGAAATGGAA CAGACATCAT CTATAATTAT CATGGGATAG TTTCCTTGAA ACTAGAGGAT
    GACAGCTTCC CAACTCACAA AAGGAAGGCC AAAGTATCCA TCATTAGTCA GCCACAAAAG
    ACAATCAAAG TGGCAGAACT GCCTCAAGCA GATAAGGTGG AATCCACAAC TGACTCACAC
    TTCCCCAGAC AGGACCAGTT GCCCTCATTT CCAAAGAACT GCACTCTGGA ATTAAAGGGA
    CTCTTCCATT TTGAAGAAGG CATCCAGAAG CTGTATCAGT GCAATGGGAT TGCCTGGAAA
    GCCTGGAGTC CCCAAACCAA GGATGTGGAA GAAAAATCCT GTCCAGCCGG GTGGCACCAG
    CACTCAGGCT ACTGTCACAT CTTGATCACA GAGCAGAAAG GCACCTGGAA TGCGGCTGCC
    CAAGCTTGCA GGGAACAATA CCTGGGCAAC CTTGTAACTG TATTCTCCAG GCAGCACATG
    CGGTGGCTCT GGGACATTGG TGAGAGAAAG TCCTTTTGGA TAGGTTTGAA CGACCAAGTG
    CATGCTGGCC ACTGGGAGTG GATCGGTGGT GAACCTGTTG CCTTCACCAA TGGGAGAAGA
    GGGCCCTCTC AACGCTCCAA GCTTGGAAAG AGCTGTGTTT TGGTTCAAAG ACAAGGGAAA
    TGGCAAACAA AAGATTGTAG GAGAGCCAAA CCTCATAATT ATGTGTGTTC CAGAAAACTC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016816119.1
    CDS155..5797
    Translation

    Target ORF information:

    RefSeq Version XM_016960630.1
    Organism Pan troglodytes(chimpanzee)
    Definition Pan troglodytes FRAS1 related extracellular matrix 1 (FREM1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_016960630.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGGCCATGT TTATTCTGGA AGTGGATCAG TTCATCCTGA CCTCCTTGAC TACATCAGTT 
    CTGGACTGTG AAGAAGATGA GACCCCTAAA CCCTTGCTGG TGTTCAACAT TACTAAAGCC
    CCGCTCCAGG GCTATGTGAC TCACCTGTTG GATCACACCA GACCAATCTC CTCATTCACC
    TGGAAAGATC TCAGTGACAT GCAGATCGCC TATCAGCCAC CAAACAGCAG CCATTCTGAG
    AGGAGACATG ATGAGGTAGA ATTGGAGGTA TACGACTTCT TCTTTGAAAG GAGTGCACCT
    ATGACAGTCC ACATCTCCAT CAGAACAGCA GATACAAATG CCCCCCGTGT ATCCTGGAAT
    ACAGGTCTGA ATCTCCTTGA GGGGCAGTCT CGAGCCATCA CTTGGGAACA GTTTCAGGTT
    GTCGACAATG ACGACATCGG TGCTGTCCGG CTAGTCACCG TTGGTGGCCT GCAGCATGGA
    TGGCTGACTT TAAGAGGGGG GAAAGGATTT CTCTTCACCG TGGCTGACCT CCAGGCTGGA
    GTTGTTCGCT ATCATCATGA TGACAGCGAC TCCACCAAAG ACTTCGTGGT CTTCCGGATA
    TTTGATGGCC ATCACAGCAT CCGTCACAAA TTCCCCATCA ACGTCTTGCC CAAAGATGAT
    AGTCCCCCGT TCCTCATAAC CAATGTTGTC ATTGAACTGG AGGAGGGGCA GACCATCCTG
    ATCCAGGGAT CCATGCTGCG AGCTTCAGAT GTGGATGCCA GTGATGACTA CATCTTCTTC
    AATATCACAA AGCCTCCACA GGCTGGGGAG ATCATGAAGA AGCCAGGGCC AGGACTGATA
    GGCTATCCTG TCCCTGGCTT CCTTCAGAGG GATTTGTTTA ATGGAATCAT TTATTATCGT
    CATTTTGGTG GAGAAATCTT TGAAGATTCT TTTCAATTTG TCCTGTGGGA CAGCCATGAA
    CCTCCAAATC TTTCAGTGCC ACAGGTGGCA ACAATCCATG TAACTCCAGT GGATGACCAG
    CTTCCAAAAG AGGCTCCTGG AGTTTCTCGG CATTTGGTTG TCAAGGAAAC TGAGGTGGCC
    TATATAACTA AGAAACAGCT ACATTTTATA GATTCAGAAT CATATGACAG GGAGCTGGTC
    TACACGATAA CTACTCCTCC ATTTTTCTCC TTCAGCCACA GACACTTGGA TGCTGGGAAA
    TTATTTATGG TGGACAGCAT ACCAAAAGTA GTTAAGAATC CTACGGCCCT GGAGCTGAGG
    TCATTCACTC AGCATGCTGT GAACTATATG AAAGTGGCCT ACATGCCCCC CATGCAAGAC
    ATTGGTCCCC ATTGCAGAGA TGTCCAGTTC ACATTTTCTG TCAGTAACCA ACATGGCGGC
    ACTTTGCATG GGATCTGCTT TAACATTACA ATCCTCCCAG TGGACAATCA AGTTCCTGAG
    GCGTTCACCA ACCCTCTGAA AGTGACTGAG GGAGGTCAAA GCATCATCAG CACAGAGCAC
    ATTCTGATTT CTGATGCAGA TACCAAGCTG GACAATATTG ACCTCTCCCT GCGGGAATTG
    CCTCTGCACG GAAGGGTGGA GCTGAATGGA TTTCCTCTAA ATTCAGGGGG CACATTTTCT
    TGGGGCGATC TCCATACCTT AAAAGTTAGG TATCAACATG ATGGAACTGA AGTTCTTCAG
    GATGACCTAC TCTTGGAGGT CACCGATGGC ACAAATTCAG CAGAATTTGT TCTACATGTT
    GAGGTATTCC CTGTCAACGA TGAGCCACCA GTCTTAAAGG CTGACCTCAT GCCTGTCATG
    AATTGCTCAG AGGGAGGAGA GGTGGTCATC ACCTCTGAAT ACATTTTTGC TACTGATGTG
    GACAGCGATA ACTTGAAGTT GATGTTTGTG ATTGCTCGCG AACCTCAGCA TGGGGTGGTG
    AGGAGAGCTG GAGTCACAGT GGATCAGTTC TCTCAGAGAG ATGTTATCTC AGAGGCCGTG
    ACATACAAAC ACACAGGTGG CGAGATTGGC ATGATGCCCT GTTTTGACAC CATTACATTG
    GTGGTTTCGG ATGGAGAGGC TGGCCCTTTT GTGAATGGCT GTTGCTACAA TGGACCCAAT
    CCATCTGTTC CGCTTCATGC GTCCTTTCCA GTGTATGATC TCAACATCAC GGTATACCCA
    GTAGACAACC AGCCACCTTC CGTTGCAATA GGTCCCATGT TTGTTGTAGA TGAGGGCTGC
    TCAACAGCCC TTACTGTTAA CCATTTGTCC GCCACTGACC CTGACACTGC AGCAGATGAC
    TTGGAATTTG TTTTGGTTTC TCCTCCTCAG TTTGGCTACC TCGAAAATAT ACTCCCTTCT
    GTGGGTTTTG AAAAAAGCAA TATTGGCATA AGTATAGATT CATTTCAGTG GAAAGACATG
    AACGCTTTTC ACATTAACTA TGTGCAGTCC AGGCATCTGA GGATAGAACC AACTGCCGAC
    CAGTTCACGG TGTACGTCAC AGATGGGAAG CAACACTCCT TGGAGATACC ATTTTCTATT
    ATAATCAACC CCACAAATGA TGAAGCTCCT GACTTTGTAG TGCAGAATAT TACTGTGTGC
    GAGGGTCAGA TGAAAGAGCT GGACTCTTCC ATCATCAGCG CTGTGGACCT GGACATTCCC
    CAGGATGCCC TGCTGTTCAG CATCACTCAA AAGCCACGCC ATGGCCTCCT CATCGATAGG
    GGGTTTAGCA AAGACTTCTC TGAGAATAAG CAGCCAGCCA ACCCTCACCA GAAACATGTA
    CCTGTTCACA GTTTTTCCAT GGAACTCCTC AAGACTGGAA TGAGGTTGAC GTACATGCAT
    GATGACTCAG AGAGCCTTGC TGATGATTTT ACAATCCGTT TGTCAGATGG GAAACATAAG
    ATACTTAAAA CCATTTCAAT AGAGGTCATC CCAGTTAATG ATGAAAAACC AATGCTGAGC
    AAGAAGGCTG AAATTGCAAT GAATATGGGT GAAACTCGTA TTATTTCCAG TGCTATTCTT
    TCAGCCATAG ATGAAGACTC ACCCAGGGAG AAGATTTACT ATGTATTTGA AAGGCTTCCC
    CAAAATGGGC AACTTCAGCT TAAGATAGGG AGGGACTGGG TTCCTCTCTC CCCTGGCATG
    AAATGCACTC AGGAGGAAGT GGATCTGAAC TTGCTGAGAT ACACACACAC CGGGGCAATG
    GATTCCCAGA ATCAAGATAG CTTCACCTTC TACCTTTGGG ATGGCAACAA CAGGTCCCCT
    GCTCTTGACT GTCAAATCAC CATCAAGGAC ATGGAAAAAG GTGATATTGT CATCCTTACA
    AAACCACTCG TGGTGTCTAA AGGTGATAGA GGTTTCTTAA CAACCACCAC TCTCCTGGCT
    GTGGACGGAA CAGACAAGCC TGAGGAACTG CTCTATGTCA TCACCTCCCC TCCACGATAT
    GGCCAGATCG AATATGTTCA CTATCCTGGA GTTCCCATTA CAAACTTCAG CCAAATGGAT
    ATAGCGGGGC AGACAGTTTG CTATGTACAC AAGAGCAAGG TGACTGTCTC CAGTGACAGA
    TTCAGATTCA TCATCAGCAA TGGACTGCGG ACCGAGCACG GGGTGTTTGA GATCACACTG
    GAGACTGTGG ACAGAGCCCT GCCTGTGGTA ACCAGGAACA AGGGGTTGAG ACTGGCCCAA
    GGGGCCGTGG GCCTGCTTTC CCCTGACCTC CTTCAGCTGA CCGACCCTGA TACACCTGTG
    GAGAACCTCA CCTTCCTCTT GGTTCAGCTT CCCCAGCATG GCCAGCTCTA CCTGTGGGGG
    ACAGGGCTAC TTCAACACAA TTTCACCCAG CAGGATGTGG ACAGCAGGAA TGTGGCCTAT
    CGGCACTCAG GAGGGGACTC CCAGACTGAC TGCTTTACTT TCATGGCCAC AGACGGGACA
    AACCAAGGCT TTATTGTGAA TGGGAGAGTA TGGGAAGAAC CTGTTTTATT CACCATTCAG
    GTAGACCAAT TGGACAAAAC AGCTCCTCGT ATCACACTCT TGCATTCCCC TTCTCAAGTG
    GGGCTCCTGA AAAATGGCTG CTACGGGATT TACATCACTT CCCGCGTGTT GAAGGCATCA
    GACCCTGACA CTGAGGACGA TCAGATCATC TTTAAAATTC TACAAGGCCC AAAACATGGA
    CATCTGGAGA ACACAACAAC AGGTGAATTT ATCCATGAGA AATTTAGCCA AAAGGACTTA
    AACAGTAAGA CTATTCTTTA CATCATAAAC CCATCTTTGG AAGTAAATTC AGATACCGTG
    GAATTTCAAA TCATGGACCC CACAGGGAAC TCGGCCACTC CTCAAATTTT GGAACTGAAG
    TGGTCTCATA TTGAATGGTC ACAGACCGAA TATGAAGTCT GTGAGAATGT GGGTGTGTTG
    CCCTTGGAAA TTATCAGAAG GGGATATTCC ATGGACTCGG CCTTTGTGGG TATAAAGGTC
    AACCAAGTGT CAGCTGCAGA TGGAAAAGAT TTCACCGTGA TTCCATCTAA ACTGATTCAG
    TTTGACCCAG GAATGTCAAC TAAGATGTGG AATATAGCAA TTACCTATGA CGGATTAGAG
    GAAGATGATG AGGTCTTTGA AGTAATTCTG AACTCCCCTG TGAATGCAGT TCTTGGCACA
    AAGACAAAAG CTGCAGTGAA AATTTTGGAC TCAAAAGGAG GACAATGCCA TCCTTCATAT
    TCCTCCAACC AAAGCAAGCA CAGCACATGG GAGAAGGGCA TTTGGCATCT GCTGCCCCCA
    GGGTCTTCCT CATCCACCAC TTCTGGTTCC TTTCATCTGG AAAGAAGACC TCTTCCATCT
    TCCATGCAGC TAGCAGTCAC CAGGGGAGAC ACCCTGCGGG GCTTTGATTC TACAGATCTT
    TCTCAAAGGA AGCTTAGGAC CCGTGGGAAT GGCAAAACAG TTCGTCCATC CTCTGTTTAT
    AGAAATGGAA CAGACATCAT CTATAATTAT CATGGGATAG TTTCCTTGAA ACTAGAGGAT
    GACAGCTTCC CAACTCACAA AAGGAAGGCC AAAGTATCCA TCATTAGTCA GCCACAAAAG
    ACAATCAAAG TGGCAGAACT GCCTCAAGCA GATAAGGTGG AATCCACAAC TGACTCACAC
    TTCCCCAGAC AGGACCAGTT GCCCTCATTT CCAAAGAACT GCACTCTGGA ATTAAAGGGA
    CTCTTCCATT TTGAAGAAGG CATCCAGAAG CTGTATCAGT GCAATGGGAT TGCCTGGAAA
    GCCTGGAGTC CCCAAACCAA GGATGTGGAA GAAAAATCCT GTCCAGCCGG GTGGCACCAG
    CACTCAGGCT ACTGTCACAT CTTGATCACA GAGCAGAAAG GCACCTGGAA TGCGGCTGCC
    CAAGCTTGCA GGGAACAATA CCTGGGCAAC CTTGTAACTG TATTCTCCAG GCAGCACATG
    CGGTGGCTCT GGGACATTGG TGAGAGAAAG TCCTTTTGGA TAGGTTTGAA CGACCAAGTG
    CATGCTGGCC ACTGGGAGTG GATCGGTGGT GAACCTGTTG CCTTCACCAA TGGGAGAAGA
    GGGCCCTCTC AACGCTCCAA GCTTGGAAAG AGCTGTGTTT TGGTTCAAAG ACAAGGGAAA
    TGGCAAACAA AAGATTGTAG GAGAGCCAAA CCTCATAATT ATGTGTGTTC CAGAAAACTC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OPc88760
    Clone ID Related Accession (Same CDS sequence) XM_016960631.1
    Accession Version XM_016960631.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5484bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-01
    Organism Pan troglodytes(chimpanzee)
    Product FRAS1-related extracellular matrix protein 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006476.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGAACTCTC TGAGTTGGGG GGCTGCGAAT GCCGTGCTGC TGCTGCTCCT CCTGGCCTGG 
    GCCAGCCCCA CCTTCATCAG CATCAACCGT GGGGTGAGGG TGATGAAGGG CCACTCTGCC
    TTCCTGTCAG GAGATGACCT GAAGTTTGCC ATCCCTAAAG AGAAAGATGC CTGCAAAGTG
    GAAGTTGTGA TGAATGAGCC AATAACCCAG AGGGTTGGGA AACTCACTCC ACAGGTCTTT
    GACTGCCATT TCCTTCCCAA TGAAGTCAAG TATGTTCACA ATGGTTGTCC AATTCTTGAT
    GAAGACACAG TGAAGCTTAG ACTTTACAGA TTTACTGAAA GAGATACCTT CATAGAAACC
    TTTATCCTGT GGGTCTATCT CCTGGAACCA GACTGTAACA TCATCCATAT GAGTAACAAT
    GTGCTGGAGG TGCCTGAATT CAATGGCCTG TCCCAAGCGA TTGATAAAAA TCTGCTCAGA
    TTCGATTATG ATAGGATGGC TAGCCTGGAA TGTACGGTCA GCCTGGACAC TGCGAGAACT
    CGGCTGCCAG CCCATGGCCA GATGGTTCTC GGGGAGCCTC GACCAGAAGA ACCTCGTGGA
    GATCAGCCAC ACAGTTTTTT CCCAGAGTCC CAACTGAGAG CAAAACTGAA GTGTCCAGGT
    GGAAGCTGTA CCCCAGGATT AAAGAAAATA GGAAGCCTCA AAGTGAGCTG TGAGGAGTTC
    CTGCTGATGG GCCTTCGTTA TCAGCATCTG GATCCCCCTT CACCCAACAT TGATTATATC
    TCCATCCAAC TGGACCTCAC AGATACCAGG AGTAAAATTG TGTACAAGTC AGAGAGTGCG
    TGGCTGCCTG TCTATATCAG AGCTGGAATT CCGAATCAGA TTCCAAAGGC TGCATTCATG
    GCCATGTTTA TTCTGGAAGT GGATCAGTTC ATCCTGACCT CCTTGACTAC ATCAGTTCTG
    GACTGTGAAG AAGATGAGAC CCCTAAACCC TTGCTGGTGT TCAACATTAC TAAAGCCCCG
    CTCCAGGGCT ATGTGACTCA CCTGTTGGAT CACACCAGAC CAATCTCCTC ATTCACCTGG
    AAAGATCTCA GTGACATGCA GATCGCCTAT CAGCCACCAA ACAGCAGCCA TTCTGAGAGG
    AGACATGATG AGGTAGAATT GGAGGTATAC GACTTCTTCT TTGAAAGGAG TGCACCTATG
    ACAGTCCACA TCTCCATCAG AACAGCAGAT ACAAATGCCC CCCGTGTATC CTGGAATACA
    GGTCTGAATC TCCTTGAGGG GCAGTCTCGA GCCATCACTT GGGAACAGTT TCAGGTTGTC
    GACAATGACG ACATCGGTGC TGTCCGGCTA GTCACCGTTG GTGGCCTGCA GCATGGATGG
    CTGACTTTAA GAGGGGGGAA AGGATTTCTC TTCACCGTGG CTGACCTCCA GGCTGGAGTT
    GTTCGCTATC ATCATGATGA CAGCGACTCC ACCAAAGACT TCGTGGTCTT CCGGATATTT
    GATGGCCATC ACAGCATCCG TCACAAATTC CCCATCAACG TCTTGCCCAA AGATGATAGT
    CCCCCGTTCC TCATAACCAA TGTTGTCATT GAACTGGAGG AGGGGCAGAC CATCCTGATC
    CAGGGATCCA TGCTGCGAGC TTCAGATGTG GATGCCAGTG ATGACTACAT CTTCTTCAAT
    ATCACAAAGC CTCCACAGGC TGGGGAGATC ATGAAGAAGC CAGGGCCAGG ACTGATAGGC
    TATCCTGTCC CTGGCTTCCT TCAGAGGGAT TTGTTTAATG GAATCATTTA TTATCGTCAT
    TTTGGTGGAG AAATCTTTGA AGATTCTTTT CAATTTGTCC TGTGGGACAG CCATGAACCT
    CCAAATCTTT CAGTGCCACA GGTGGCAACA ATCCATGTAA CTCCAGTGGA TGACCAGCTT
    CCAAAAGAGG CTCCTGGAGT TTCTCGGCAT TTGGTTGTCA AGGAAACTGA GGTGGCCTAT
    ATAACTAAGA AACAGCTACA TTTTATAGAT TCAGAATCAT ATGACAGGGA GCTGGTCTAC
    ACGATAACTA CTCCTCCATT TTTCTCCTTC AGCCACAGAC ACTTGGATGC TGGGAAATTA
    TTTATGGTGG ACAGCATACC AAAAGTAGTT AAGAATCCTA CGGCCCTGGA GCTGAGGTCA
    TTCACTCAGC ATGCTGTGAA CTATATGAAA GTGGCCTACA TGCCCCCCAT GCAAGACATT
    GGTCCCCATT GCAGAGATGT CCAGTTCACA TTTTCTGTCA GTAACCAACA TGGCGGCACT
    TTGCATGGGA TCTGCTTTAA CATTACAATC CTCCCAGTGG ACAATCAAGT TCCTGAGGCG
    TTCACCAACC CTCTGAAAGT GACTGAGGGA GGTCAAAGCA TCATCAGCAC AGAGCACATT
    CTGATTTCTG ATGCAGATAC CAAGCTGGAC AATATTGACC TCTCCCTGCG GGAATTGCCT
    CTGCACGGAA GGGTGGAGCT GAATGGATTT CCTCTAAATT CAGGGGGCAC ATTTTCTTGG
    GGCGATCTCC ATACCTTAAA AGTTAGGTAT CAACATGATG GAACTGAAGT TCTTCAGGAT
    GACCTACTCT TGGAGGTCAC CGATGGCACA AATTCAGCAG AATTTGTTCT ACATGTTGAG
    GTATTCCCTG TCAACGATGA GCCACCAGTC TTAAAGGCTG ACCTCATGCC TGTCATGAAT
    TGCTCAGAGG GAGGAGAGGT GGTCATCACC TCTGAATACA TTTTTGCTAC TGATGTGGAC
    AGCGATAACT TGAAGTTGAT GTTTGTGATT GCTCGCGAAC CTCAGCATGG GGTGGTGAGG
    AGAGCTGGAG TCACAGTGGA TCAGTTCTCT CAGAGAGATG TTATCTCAGA GGCCGTGACA
    TACAAACACA CAGGTGGCGA GATTGGCATG ATGCCCTGTT TTGACACCAT TACATTGGTG
    GTTTCGGATG GAGAGGCTGG CCCTTTTGTG AATGGCTGTT GCTACAATGG ACCCAATCCA
    TCTGTTCCGC TTCATGCGTC CTTTCCAGTG TATGATCTCA ACATCACGGT ATACCCAGTA
    GACAACCAGC CACCTTCCGT TGCAATAGGT CCCATGTTTG TTGTAGATGA GGGCTGCTCA
    ACAGCCCTTA CTGTTAACCA TTTGTCCGCC ACTGACCCTG ACACTGCAGC AGATGACTTG
    GAATTTGTTT TGGTTTCTCC TCCTCAGTTT GGCTACCTCG AAAATATACT CCCTTCTGTG
    GGTTTTGAAA AAAGCAATAT TGGCATAAGT ATAGATTCAT TTCAGTGGAA AGACATGAAC
    GCTTTTCACA TTAACTATGT GCAGTCCAGG CATCTGAGGA TAGAACCAAC TGCCGACCAG
    TTCACGGTGT ACGTCACAGA TGGGAAGCAA CACTCCTTGG AGATACCATT TTCTATTATA
    ATCAACCCCA CAAATGATGA AGCTCCTGAC TTTGTAGTGC AGAATATTAC TGTGTGCGAG
    GGTCAGATGA AAGAGCTGGA CTCTTCCATC ATCAGCGCTG TGGACCTGGA CATTCCCCAG
    GATGCCCTGC TGTTCAGCAT CACTCAAAAG CCACGCCATG GCCTCCTCAT CGATAGGGGG
    TTTAGCAAAG ACTTCTCTGA GAATAAGCAG CCAGCCAACC CTCACCAGAA ACATGTACCT
    GTTCACAGTT TTTCCATGGA ACTCCTCAAG ACTGGAATGA GGTTGACGTA CATGCATGAT
    GACTCAGAGA GCCTTGCTGA TGATTTTACA ATCCGTTTGT CAGATGGGAA ACATAAGATA
    CTTAAAACCA TTTCAATAGA GGTCATCCCA GTTAATGATG AAAAACCAAT GCTGAGCAAG
    AAGGCTGAAA TTGCAATGAA TATGGGTGAA ACTCGTATTA TTTCCAGTGC TATTCTTTCA
    GCCATAGATG AAGACTCACC CAGGGAGAAG ATTTACTATG TATTTGAAAG GCTTCCCCAA
    AATGGGCAAC TTCAGCTTAA GATAGGGAGG GACTGGGTTC CTCTCTCCCC TGGCATGAAA
    TGCACTCAGG AGGAAGTGGA TCTGAACTTG CTGAGATACA CACACACCGG GGCAATGGAT
    TCCCAGAATC AAGATAGCTT CACCTTCTAC CTTTGGGATG GCAACAACAG GTCCCCTGCT
    CTTGACTGTC AAATCACCAT CAAGGACATG GAAAAAGGTG ATATTGTCAT CCTTACAAAA
    CCACTCGTGG TGTCTAAAGG TGATAGAGGT TTCTTAACAA CCACCACTCT CCTGGCTGTG
    GACGGAACAG ACAAGCCTGA GGAACTGCTC TATGTCATCA CCTCCCCTCC ACGATATGGC
    CAGATCGAAT ATGTTCACTA TCCTGGAGTT CCCATTACAA ACTTCAGCCA AATGGATATA
    GCGGGGCAGA CAGTTTGCTA TGTACACAAG AGCAAGGTGA CTGTCTCCAG TGACAGATTC
    AGATTCATCA TCAGCAATGG ACTGCGGACC GAGCACGGGG TGTTTGAGAT CACACTGGAG
    ACTGTGGACA GAGCCCTGCC TGTGGTAACC AGGAACAAGG GGTTGAGACT GGCCCAAGGG
    GCCGTGGGCC TGCTTTCCCC TGACCTCCTT CAGCTGACCG ACCCTGATAC ACCTGTGGAG
    AACCTCACCT TCCTCTTGGT TCAGCTTCCC CAGCATGGCC AGCTCTACCT GTGGGGGACA
    GGGCTACTTC AACACAATTT CACCCAGCAG GATGTGGACA GCAGGAATGT GGCCTATCGG
    CACTCAGGAG GGGACTCCCA GACTGACTGC TTTACTTTCA TGGCCACAGA CGGGACAAAC
    CAAGGCTTTA TTGTGAATGG GAGAGTATGG GAAGAACCTG TTTTATTCAC CATTCAGGTA
    GACCAATTGG ACAAAACAGC TCCTCGTATC ACACTCTTGC ATTCCCCTTC TCAAGTGGGG
    CTCCTGAAAA ATGGCTGCTA CGGGATTTAC ATCACTTCCC GCGTGTTGAA GGCATCAGAC
    CCTGACACTG AGGACGATCA GATCATCTTT AAAATTCTAC AAGGCCCAAA ACATGGACAT
    CTGGAGAACA CAACAACAGG TGAATTTATC CATGAGAAAT TTAGCCAAAA GGACTTAAAC
    AGTAAGACTA TTCTTTACAT CATAAACCCA TCTTTGGAAG TAAATTCAGA TACCGTGGAA
    TTTCAAATCA TGGACCCCAC AGGGAACTCG GCCACTCCTC AAATTTTGGA ACTGAAGTGG
    TCTCATATTG AATGGTCACA GACCGAATAT GAAGTCTGTG AGAATGTGGG TGTGTTGCCC
    TTGGAAATTA TCAGAAGGGG ATATTCCATG GACTCGGCCT TTGTGGGTAT AAAGGTCAAC
    CAAGTGTCAG CTGCAGATGG AAAAGATTTC ACCGTGATTC CATCTAAACT GATTCAGTTT
    GACCCAGGTG CAAGTGGAAA TAAAAAAGGC TGGTACTTGC TCTCAAGTGT GTTCCATTGT
    GTGAGAAACA CAGACAAGAA TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016816120.1
    CDS2128..7611
    Translation

    Target ORF information:

    RefSeq Version XM_016960631.1
    Organism Pan troglodytes(chimpanzee)
    Definition Pan troglodytes FRAS1 related extracellular matrix 1 (FREM1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_016960631.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGAACTCTC TGAGTTGGGG GGCTGCGAAT GCCGTGCTGC TGCTGCTCCT CCTGGCCTGG 
    GCCAGCCCCA CCTTCATCAG CATCAACCGT GGGGTGAGGG TGATGAAGGG CCACTCTGCC
    TTCCTGTCAG GAGATGACCT GAAGTTTGCC ATCCCTAAAG AGAAAGATGC CTGCAAAGTG
    GAAGTTGTGA TGAATGAGCC AATAACCCAG AGGGTTGGGA AACTCACTCC ACAGGTCTTT
    GACTGCCATT TCCTTCCCAA TGAAGTCAAG TATGTTCACA ATGGTTGTCC AATTCTTGAT
    GAAGACACAG TGAAGCTTAG ACTTTACAGA TTTACTGAAA GAGATACCTT CATAGAAACC
    TTTATCCTGT GGGTCTATCT CCTGGAACCA GACTGTAACA TCATCCATAT GAGTAACAAT
    GTGCTGGAGG TGCCTGAATT CAATGGCCTG TCCCAAGCGA TTGATAAAAA TCTGCTCAGA
    TTCGATTATG ATAGGATGGC TAGCCTGGAA TGTACGGTCA GCCTGGACAC TGCGAGAACT
    CGGCTGCCAG CCCATGGCCA GATGGTTCTC GGGGAGCCTC GACCAGAAGA ACCTCGTGGA
    GATCAGCCAC ACAGTTTTTT CCCAGAGTCC CAACTGAGAG CAAAACTGAA GTGTCCAGGT
    GGAAGCTGTA CCCCAGGATT AAAGAAAATA GGAAGCCTCA AAGTGAGCTG TGAGGAGTTC
    CTGCTGATGG GCCTTCGTTA TCAGCATCTG GATCCCCCTT CACCCAACAT TGATTATATC
    TCCATCCAAC TGGACCTCAC AGATACCAGG AGTAAAATTG TGTACAAGTC AGAGAGTGCG
    TGGCTGCCTG TCTATATCAG AGCTGGAATT CCGAATCAGA TTCCAAAGGC TGCATTCATG
    GCCATGTTTA TTCTGGAAGT GGATCAGTTC ATCCTGACCT CCTTGACTAC ATCAGTTCTG
    GACTGTGAAG AAGATGAGAC CCCTAAACCC TTGCTGGTGT TCAACATTAC TAAAGCCCCG
    CTCCAGGGCT ATGTGACTCA CCTGTTGGAT CACACCAGAC CAATCTCCTC ATTCACCTGG
    AAAGATCTCA GTGACATGCA GATCGCCTAT CAGCCACCAA ACAGCAGCCA TTCTGAGAGG
    AGACATGATG AGGTAGAATT GGAGGTATAC GACTTCTTCT TTGAAAGGAG TGCACCTATG
    ACAGTCCACA TCTCCATCAG AACAGCAGAT ACAAATGCCC CCCGTGTATC CTGGAATACA
    GGTCTGAATC TCCTTGAGGG GCAGTCTCGA GCCATCACTT GGGAACAGTT TCAGGTTGTC
    GACAATGACG ACATCGGTGC TGTCCGGCTA GTCACCGTTG GTGGCCTGCA GCATGGATGG
    CTGACTTTAA GAGGGGGGAA AGGATTTCTC TTCACCGTGG CTGACCTCCA GGCTGGAGTT
    GTTCGCTATC ATCATGATGA CAGCGACTCC ACCAAAGACT TCGTGGTCTT CCGGATATTT
    GATGGCCATC ACAGCATCCG TCACAAATTC CCCATCAACG TCTTGCCCAA AGATGATAGT
    CCCCCGTTCC TCATAACCAA TGTTGTCATT GAACTGGAGG AGGGGCAGAC CATCCTGATC
    CAGGGATCCA TGCTGCGAGC TTCAGATGTG GATGCCAGTG ATGACTACAT CTTCTTCAAT
    ATCACAAAGC CTCCACAGGC TGGGGAGATC ATGAAGAAGC CAGGGCCAGG ACTGATAGGC
    TATCCTGTCC CTGGCTTCCT TCAGAGGGAT TTGTTTAATG GAATCATTTA TTATCGTCAT
    TTTGGTGGAG AAATCTTTGA AGATTCTTTT CAATTTGTCC TGTGGGACAG CCATGAACCT
    CCAAATCTTT CAGTGCCACA GGTGGCAACA ATCCATGTAA CTCCAGTGGA TGACCAGCTT
    CCAAAAGAGG CTCCTGGAGT TTCTCGGCAT TTGGTTGTCA AGGAAACTGA GGTGGCCTAT
    ATAACTAAGA AACAGCTACA TTTTATAGAT TCAGAATCAT ATGACAGGGA GCTGGTCTAC
    ACGATAACTA CTCCTCCATT TTTCTCCTTC AGCCACAGAC ACTTGGATGC TGGGAAATTA
    TTTATGGTGG ACAGCATACC AAAAGTAGTT AAGAATCCTA CGGCCCTGGA GCTGAGGTCA
    TTCACTCAGC ATGCTGTGAA CTATATGAAA GTGGCCTACA TGCCCCCCAT GCAAGACATT
    GGTCCCCATT GCAGAGATGT CCAGTTCACA TTTTCTGTCA GTAACCAACA TGGCGGCACT
    TTGCATGGGA TCTGCTTTAA CATTACAATC CTCCCAGTGG ACAATCAAGT TCCTGAGGCG
    TTCACCAACC CTCTGAAAGT GACTGAGGGA GGTCAAAGCA TCATCAGCAC AGAGCACATT
    CTGATTTCTG ATGCAGATAC CAAGCTGGAC AATATTGACC TCTCCCTGCG GGAATTGCCT
    CTGCACGGAA GGGTGGAGCT GAATGGATTT CCTCTAAATT CAGGGGGCAC ATTTTCTTGG
    GGCGATCTCC ATACCTTAAA AGTTAGGTAT CAACATGATG GAACTGAAGT TCTTCAGGAT
    GACCTACTCT TGGAGGTCAC CGATGGCACA AATTCAGCAG AATTTGTTCT ACATGTTGAG
    GTATTCCCTG TCAACGATGA GCCACCAGTC TTAAAGGCTG ACCTCATGCC TGTCATGAAT
    TGCTCAGAGG GAGGAGAGGT GGTCATCACC TCTGAATACA TTTTTGCTAC TGATGTGGAC
    AGCGATAACT TGAAGTTGAT GTTTGTGATT GCTCGCGAAC CTCAGCATGG GGTGGTGAGG
    AGAGCTGGAG TCACAGTGGA TCAGTTCTCT CAGAGAGATG TTATCTCAGA GGCCGTGACA
    TACAAACACA CAGGTGGCGA GATTGGCATG ATGCCCTGTT TTGACACCAT TACATTGGTG
    GTTTCGGATG GAGAGGCTGG CCCTTTTGTG AATGGCTGTT GCTACAATGG ACCCAATCCA
    TCTGTTCCGC TTCATGCGTC CTTTCCAGTG TATGATCTCA ACATCACGGT ATACCCAGTA
    GACAACCAGC CACCTTCCGT TGCAATAGGT CCCATGTTTG TTGTAGATGA GGGCTGCTCA
    ACAGCCCTTA CTGTTAACCA TTTGTCCGCC ACTGACCCTG ACACTGCAGC AGATGACTTG
    GAATTTGTTT TGGTTTCTCC TCCTCAGTTT GGCTACCTCG AAAATATACT CCCTTCTGTG
    GGTTTTGAAA AAAGCAATAT TGGCATAAGT ATAGATTCAT TTCAGTGGAA AGACATGAAC
    GCTTTTCACA TTAACTATGT GCAGTCCAGG CATCTGAGGA TAGAACCAAC TGCCGACCAG
    TTCACGGTGT ACGTCACAGA TGGGAAGCAA CACTCCTTGG AGATACCATT TTCTATTATA
    ATCAACCCCA CAAATGATGA AGCTCCTGAC TTTGTAGTGC AGAATATTAC TGTGTGCGAG
    GGTCAGATGA AAGAGCTGGA CTCTTCCATC ATCAGCGCTG TGGACCTGGA CATTCCCCAG
    GATGCCCTGC TGTTCAGCAT CACTCAAAAG CCACGCCATG GCCTCCTCAT CGATAGGGGG
    TTTAGCAAAG ACTTCTCTGA GAATAAGCAG CCAGCCAACC CTCACCAGAA ACATGTACCT
    GTTCACAGTT TTTCCATGGA ACTCCTCAAG ACTGGAATGA GGTTGACGTA CATGCATGAT
    GACTCAGAGA GCCTTGCTGA TGATTTTACA ATCCGTTTGT CAGATGGGAA ACATAAGATA
    CTTAAAACCA TTTCAATAGA GGTCATCCCA GTTAATGATG AAAAACCAAT GCTGAGCAAG
    AAGGCTGAAA TTGCAATGAA TATGGGTGAA ACTCGTATTA TTTCCAGTGC TATTCTTTCA
    GCCATAGATG AAGACTCACC CAGGGAGAAG ATTTACTATG TATTTGAAAG GCTTCCCCAA
    AATGGGCAAC TTCAGCTTAA GATAGGGAGG GACTGGGTTC CTCTCTCCCC TGGCATGAAA
    TGCACTCAGG AGGAAGTGGA TCTGAACTTG CTGAGATACA CACACACCGG GGCAATGGAT
    TCCCAGAATC AAGATAGCTT CACCTTCTAC CTTTGGGATG GCAACAACAG GTCCCCTGCT
    CTTGACTGTC AAATCACCAT CAAGGACATG GAAAAAGGTG ATATTGTCAT CCTTACAAAA
    CCACTCGTGG TGTCTAAAGG TGATAGAGGT TTCTTAACAA CCACCACTCT CCTGGCTGTG
    GACGGAACAG ACAAGCCTGA GGAACTGCTC TATGTCATCA CCTCCCCTCC ACGATATGGC
    CAGATCGAAT ATGTTCACTA TCCTGGAGTT CCCATTACAA ACTTCAGCCA AATGGATATA
    GCGGGGCAGA CAGTTTGCTA TGTACACAAG AGCAAGGTGA CTGTCTCCAG TGACAGATTC
    AGATTCATCA TCAGCAATGG ACTGCGGACC GAGCACGGGG TGTTTGAGAT CACACTGGAG
    ACTGTGGACA GAGCCCTGCC TGTGGTAACC AGGAACAAGG GGTTGAGACT GGCCCAAGGG
    GCCGTGGGCC TGCTTTCCCC TGACCTCCTT CAGCTGACCG ACCCTGATAC ACCTGTGGAG
    AACCTCACCT TCCTCTTGGT TCAGCTTCCC CAGCATGGCC AGCTCTACCT GTGGGGGACA
    GGGCTACTTC AACACAATTT CACCCAGCAG GATGTGGACA GCAGGAATGT GGCCTATCGG
    CACTCAGGAG GGGACTCCCA GACTGACTGC TTTACTTTCA TGGCCACAGA CGGGACAAAC
    CAAGGCTTTA TTGTGAATGG GAGAGTATGG GAAGAACCTG TTTTATTCAC CATTCAGGTA
    GACCAATTGG ACAAAACAGC TCCTCGTATC ACACTCTTGC ATTCCCCTTC TCAAGTGGGG
    CTCCTGAAAA ATGGCTGCTA CGGGATTTAC ATCACTTCCC GCGTGTTGAA GGCATCAGAC
    CCTGACACTG AGGACGATCA GATCATCTTT AAAATTCTAC AAGGCCCAAA ACATGGACAT
    CTGGAGAACA CAACAACAGG TGAATTTATC CATGAGAAAT TTAGCCAAAA GGACTTAAAC
    AGTAAGACTA TTCTTTACAT CATAAACCCA TCTTTGGAAG TAAATTCAGA TACCGTGGAA
    TTTCAAATCA TGGACCCCAC AGGGAACTCG GCCACTCCTC AAATTTTGGA ACTGAAGTGG
    TCTCATATTG AATGGTCACA GACCGAATAT GAAGTCTGTG AGAATGTGGG TGTGTTGCCC
    TTGGAAATTA TCAGAAGGGG ATATTCCATG GACTCGGCCT TTGTGGGTAT AAAGGTCAAC
    CAAGTGTCAG CTGCAGATGG AAAAGATTTC ACCGTGATTC CATCTAAACT GATTCAGTTT
    GACCCAGGTG CAAGTGGAAA TAAAAAAGGC TGGTACTTGC TCTCAAGTGT GTTCCATTGT
    GTGAGAAACA CAGACAAGAA TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OPc109241
    Clone ID Related Accession (Same CDS sequence) XM_024345565.1 , XM_024345568.1 , XM_024345567.1 , XM_024345569.1 , XM_024345566.1
    Accession Version XM_024345565.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6567bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-03-19
    Organism Pan troglodytes(chimpanzee)
    Product FRAS1-related extracellular matrix protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036888.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    ATGAACTCTC TGAGTTGGGG GGCTGCGAAT GCCGTGCTGC TGCTGCTCCT CCTGGCCTGG 
    GCCAGCCCCA CCTTCATCAG CATCAACCGT GGGGTGAGGG TGATGAAGGG CCACTCTGCC
    TTCCTGTCAG GAGATGACCT GAAGTTTGCC ATCCCTAAAG AGAAAGATGC CTGCAAAGTG
    GAAGTTGTGA TGAATGAGCC AATAACCCAG AGGGTTGGGA AACTCACTCC ACAGGTCTTT
    GACTGCCATT TCCTTCCCAA TGAAGTCAAG TATGTTCACA ATGGTTGTCC AATTCTTGAT
    GAAGACACAG TGAAGCTTAG ACTTTACAGA TTTACTGAAA GAGATACCTT CATAGAAACC
    TTTATCCTGT GGGTCTATCT CCTGGAACCA GACTGTAACA TCATCCATAT GAGTAACAAT
    GTGCTGGAGG TGCCTGAATT CAATGGCCTG TCCCAAGCGA TTGATAAAAA TCTGCTCAGA
    TTCGATTATG ATAGGATGGC TAGCCTGGAA TGTACGGTCA GCCTGGACAC TGCGAGAACT
    CGGCTGCCAG CCCATGGCCA GATGGTTCTC GGGGAGCCTC GACCAGAAGA ACCTCGTGGA
    GATCAGCCAC ACAGTTTTTT CCCAGAGTCC CGTATGATGT CTTTTAATGC CTTTCTGGAA
    CTGAGAGCAA AACTGAAGTG TCCAGGTGGA AGCTGTACCC CAGGATTAAA GAAAATAGGA
    AGCCTCAAAG TGAGCTGTGA GGAGTTCCTG CTGATGGGCC TTCGTTATCA GCATCTGGAT
    CCCCCTTCAC CCAACATTGA TTATATCTCC ATCCAACTGG ACCTCACAGA TACCAGGAGT
    AAAATTGTGT ACAAGTCAGA GAGTGCGTGG CTGCCTGTCT ATATCAGAGC TGGAATTCCG
    AATCAGATTC CAAAGGCTGC ATTCATGGCC ATGTTTATTC TGGAAGTGGA TCAGTTCATC
    CTGACCTCCT TGACTACATC AGTTCTGGAC TGTGAAGAAG ATGAGACCCC TAAACCCTTG
    CTGGTGTTCA ACATTACTAA AGCCCCGCTC CAGGGCTATG TGACTCACCT GTTGGATCAC
    ACCAGACCAA TCTCCTCATT CACCTGGAAA GATCTCAGTG ACATGCAGAT CGCCTATCAG
    CCACCAAACA GCAGCCATTC TGAGAGGAGA CATGATGAGG TAGAATTGGA GGTATACGAC
    TTCTTCTTTG AAAGGAGTGC ACCTATGACA GTCCACATCT CCATCAGAAC AGCAGATACA
    AATGCCCCCC GTGTATCCTG GAATACAGGT CTGAATCTCC TTGAGGGGCA GTCTCGAGCC
    ATCACTTGGG AACAGTTTCA GGTTGTCGAC AATGACGACA TCGGTGCTGT CCGGCTAGTC
    ACCGTTGGTG GCCTGCAGCA TGGATGGCTG ACTTTAAGAG GGGGGAAAGG ATTTCTCTTC
    ACCGTGGCTG ACCTCCAGGC TGGAGTTGTT CGCTATCATC ATGATGACAG CGACTCCACC
    AAAGACTTCG TGGTCTTCCG GATATTTGAT GGCCATCACA GCATCCGTCA CAAATTCCCC
    ATCAACGTCT TGCCCAAAGA TGATAGTCCC CCGTTCCTCA TAACCAATGT TGTCATTGAA
    CTGGAGGAGG GGCAGACCAT CCTGATCCAG GGATCCATGC TGCGAGCTTC AGATGTGGAT
    GCCAGTGATG ACTACATCTT CTTCAATATC ACAAAGCCTC CACAGGCTGG GGAGATCATG
    AAGAAGCCAG GGCCAGGACT GATAGGCTAT CCTGTCCCTG GCTTCCTTCA GAGGGATTTG
    TTTAATGGAA TCATTTATTA TCGTCATTTT GGTGGAGAAA TCTTTGAAGA TTCTTTTCAA
    TTTGTCCTGT GGGACAGCCA TGAACCTCCA AATCTTTCAG TGCCACAGGT GGCAACAATC
    CATGTAACTC CAGTGGATGA CCAGCTTCCA AAAGAGGCTC CTGGAGTTTC TCGGCATTTG
    GTTGTCAAGG AAACTGAGGT GGCCTATATA ACTAAGAAAC AGCTACATTT TATAGATTCA
    GAATCATATG ACAGGGAGCT GGTCTACACG ATAACTACTC CTCCATTTTT CTCCTTCAGC
    CACAGACACT TGGATGCTGG GAAATTATTT ATGGTGGACA GCATACCAAA AGTAGTTAAG
    AATCCTACGG CCCTGGAGCT GAGGTCATTC ACTCAGCATG CTGTGAACTA TATGAAAGTG
    GCCTACATGC CCCCCATGCA AGACATTGGT CCCCATTGCA GAGATGTCCA GTTCACATTT
    TCTGTCAGTA ACCAACATGG CGGCACTTTG CATGGGATCT GCTTTAACAT TACAATCCTC
    CCAGTGGACA ATCAAGTTCC TGAGGCGTTC ACCAACCCTC TGAAAGTGAC TGAGGGAGGT
    CAAAGCATCA TCAGCACAGA GCACATTCTG ATTTCTGATG CAGATACCAA GCTGGACAAT
    ATTGACCTCT CCCTGCGGGA ATTGCCTCTG CACGGAAGGG TGGAGCTGAA TGGATTTCCT
    CTAAATTCAG GGGGCACATT TTCTTGGGGC GATCTCCATA CCTTAAAAGT TAGGTATCAA
    CATGATGGAA CTGAAGTTCT TCAGGATGAC CTACTCTTGG AGGTCACCGA TGGCACAAAT
    TCAGCAGAAT TTGTTCTACA TGTTGAGGTA TTCCCTGTCA ACGATGAGCC ACCAGTCTTA
    AAGGCTGACC TCATGCCTGT CATGAATTGC TCAGAGGGAG GAGAGGTGGT CATCACCTCT
    GAATACATTT TTGCTACTGA TGTGGACAGC GATAACTTGA AGTTGATGTT TGTGATTGCT
    CGCGAACCTC AGCATGGGGT GGTGAGGAGA GCTGGAGTCA CAGTGGATCA GTTCTCTCAG
    AGAGATGTTA TCTCAGAGGC CGTGACATAC AAACACACAG GTGGCGAGAT TGGCATGATG
    CCCTGTTTTG ACACCATTAC ATTGGTGGTT TCGGATGGAG AGGCTGGCCC TTTTGTGAAT
    GGCTGTTGCT ACAATGGACC CAATCCATCT GTTCCGCTTC ATGCGTCCTT TCCAGTGTAT
    GATCTCAACA TCACGGTATA CCCAGTAGAC AACCAGCCAC CTTCCATTGC AATAGGTCCC
    ATGTTTGTTG TAGATGAGGG CTGCTCAACA GCCCTTACTG TTAACCATTT GTCCGCCACT
    GACCCTGACA CTGCAGCAGA TGACTTGGAA TTTGTTTTGG TTTCTCCTCC TCAGTTTGGC
    TACCTCGAAA ATATACTCCC TTCTGTGGGT TTTGAAAAAA GCAATATTGG CATAAGTATA
    GATTCATTTC AGTGGAAAGA CATGAACGCT TTTCACATTA ACTATGTGCA GTCCAGGCAT
    CTGAGGATAG AACCAACTGC CGACCAGTTC ACGGTGTACG TCACAGATGG GAAGCAACAC
    TCCTTGGAGA TACCATTTTC TATTATAATC AACCCCACAA ATGATGAAGC TCCTGACTTT
    GTAGTGCAGA ATATTACTGT GTGCGAGGGT CAGATGAAAG AGCTGGACTC TTCCATCATC
    AGCGCTGTGG ACCTGGACAT TCCCCAGGAT GCCCTGCTGT TCAGCATCAC TCAAAAGCCA
    CGCCATGGCC TCCTCATCGA TAGGGGGTTT AGCAAAGACT TCTCTGAGAA TAAGCAGCCA
    GCCAACCCTC ACCAGAAACA TGTACCTGTT CACAGTTTTT CCATGGAACT CCTCAAGACT
    GGAATGAGGT TGACGTACAT GCATGATGAC TCAGAGAGCC TTGCTGATGA TTTTACAATC
    CGTTTGTCAG ATGGGAAACA TAAGATACTT AAAACCATTT CAATAGAGGT CATCCCAGTT
    AATGATGAAA AACCAATGCT GAGCAAGAAG GCTGAAATTG CAATGAATAT GGGTGAAACT
    CGTATTATTT CCAGTGCTAT TCTTTCAGCC ATAGATGAAG ACTCACCCAG GGAGAAGATT
    TACTATGTAT TTGAAAGGCT TCCCCAAAAT GGGCAACTTC AGCTTAAGAT AGGGAGGGAC
    TGGGTTCCTC TCTCCCCTGG CATGAAATGC ACTCAGGAGG AAGTGGATCT GAACTTGCTG
    AGATACACAC ACACCGGGGC AATGGATTCC CAGAATCAAG ATAGCTTCAC CTTCTACCTT
    TGGGATGGCA ACAACAGGTC CCCTGCTCTT GACTGTCAAA TCACCATCAA GGACATGGAA
    AAAGGTGATA TTGTCATCCT TACAAAACCA CTCGTGGTGT CTAAAGGTGA TAGAGGTTTC
    TTAACAACCA CCACTCTCCT GGCTGTGGAC GGAACAGACA AGCCTGAGGA ACTGCTCTAT
    GTCATCACCT CCCCTCCACG ATATGGCCAG ATCGAATATG TTCACTATCC TGGAGTTCCC
    ATTACAAACT TCAGCCAAAT GGATATAGCG GGGCAGACAG TTTGCTATGT ACACAAGAGC
    AAGGTGACTG TCTCCAGTGA CAGATTCAGA TTCATCATCA GCAATGGACT GCGGACCGAG
    CACGGGGTGT TTGAGATCAC ACTGGAGACT GTGGACAGAG CCCTGCCTGT GGTAACCAGG
    AACAAGGGGT TGAGACTGGC CCAAGGGGCC GTGGGCCTGC TTTCCCCTGA CCTCCTTCAG
    CTGACCGACC CTGATACACC TGTGGAGAAC CTCACCTTCC TCTTGGTTCA GCTTCCCCAG
    CATGGCCAGC TCTACCTGTG GGGGACAGGG CTACTTCAAC ACAATTTCAC CCAGCAGGAT
    GTGGACAGCA GGAATGTGGC CTATCGGCAC TCAGGAGGGG ACTCCCAGAC TGACTGCTTT
    ACTTTCATGG CCACAGACGG GACAAACCAA GGCTTTATTG TGAATGGGAG AGTATGGGAA
    GAACCTGTTT TATTCACCAT TCAGGTAGAC CAATTGGACA AAACAGCTCC TCGTATCACA
    CTCTTGCATT CCCCTTCTCA AGTGGGGCTC CTGAAAAATG GCTGCTACGG GATTTACATC
    ACTTCCCGCG TGTTGAAGGC ATCAGACCCT GACACTGAGG ACGATCAGAT CATCTTTAAA
    ATTCTACAAG GCCCAAAACA TGGACATCTG GAGAACACAA CAACAGGTGA ATTTATCCAT
    GAGAAATTTA GCCAAAAGGA CTTAAACAGT AAGACTATTC TTTACATCAT AAACCCATCT
    TTGGAAGTAA ATTCAGATAC CGTGGAATTT CAAATCATGG ACCCCACAGG GAACTCGGCC
    ACTCCTCAAA TTTTGGAACT GAAGTGGTCT CATATTGAAT GGTCACAGAC CGAATATGAA
    GTCTGTGAGA ATGTGGGTGT GTTGCCCTTG GAAATTATCA GAAGGGGATA TTCCATGGAC
    TCGGCCTTTG TGGGTATAAA GGTCAACCAA GTGTCAGCTG CAGATGGAAA AGATTTCACC
    GTGATTCCAT CTAAACTGAT TCAGTTTGAC CCAGGAATGT CAACTAAGAT GTGGAATATA
    GCAATTACCT ATGACGGATT AGAGGAAGAT GATGAGGTCT TTGAAGTAAT TCTGAACTCC
    CCTGTGAATG CAGTTCTTGG CACAAAGACA AAAGCTGCAG TGAAAATTTT GGACTCAAAA
    GGAGGACAAT GCCATCCTTC ATATTCCTCC AACCAAAGCA AGCACAGCAC ATGGGAGAAG
    GGCATTTGGC ATCTGCTGCC CCCAGGGTCT TCCTCATCCA CCACTTCTGG TTCCTTTCAT
    CTGGAAAGAA GACCTCTTCC ATCTTCCATG CAGCTAGCAG TCACCAGGGG AGACACCCTG
    CGGGGCTTTG ATTCTACAGA TCTTTCTCAA AGGAAGCTTA GGACCCGTGG GAATGGCAAA
    ACAGTTCGTC CATCCTCTGT TTATAGAAAT GGAACAGACA TCATCTATAA TTATCATGGG
    ATAGTTTCCT TGAAACTAGA GGATGACAGC TTCCCAACTC ACAAAAGGAA GGCCAAAGTA
    TCCATCATTA GTCAGCCACA AAAGACAATC AAAGTGGCAG AACTGCCTCA AGCAGATAAG
    GTGGAATCCA CAACTGACTC ACACTTCCCC AGACAGGACC AGTTGCCCTC ATTTCCAAAG
    AACTGCACTC TGGAATTAAA GGGACTCTTC CATTTTGAAG AAGGCATCCA GAAGCTGTAT
    CAGTGCAATG GGATTGCCTG GAAAGCCTGG AGTCCCCAAA CCAAGGATGT GGAAGAAAAA
    TCCTGTCCAG CCGGGTGGCA CCAGCACTCA GGCTACTGTC ACATCTTGAT CACAGAGCAG
    AAAGGCACCT GGAATGCGGC TGCCCAAGCT TGCAGGGAAC AATACCTGGG CAACCTTGTA
    ACTGTATTCT CCAGGCAGCA CATGCGGTGG CTCTGGGACA TTGGTGAGAG AAAGTCCTTT
    TGGATAGGTT TGAACGACCA AGTGCATGCT GGCCACTGGG AGTGGATCGG TGGTGAACCT
    GTTGCCTTCA CCAATGGGAG AAGAGGGCCC TCTCAACGCT CCAAGCTTGG AAAGAGCTGT
    GTTTTGGTTC AAAGACAAGG GAAATGGCAA ACAAAAGATT GTAGGAGAGC CAAACCTCAT
    AATTATGTGT GTTCCAGAAA ACTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024201333.1
    CDS1379..7945
    Translation

    Target ORF information:

    RefSeq Version XM_024345565.1
    Organism Pan troglodytes(chimpanzee)
    Definition Pan troglodytes FRAS1 related extracellular matrix 1 (FREM1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024345565.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    ATGAACTCTC TGAGTTGGGG GGCTGCGAAT GCCGTGCTGC TGCTGCTCCT CCTGGCCTGG 
    GCCAGCCCCA CCTTCATCAG CATCAACCGT GGGGTGAGGG TGATGAAGGG CCACTCTGCC
    TTCCTGTCAG GAGATGACCT GAAGTTTGCC ATCCCTAAAG AGAAAGATGC CTGCAAAGTG
    GAAGTTGTGA TGAATGAGCC AATAACCCAG AGGGTTGGGA AACTCACTCC ACAGGTCTTT
    GACTGCCATT TCCTTCCCAA TGAAGTCAAG TATGTTCACA ATGGTTGTCC AATTCTTGAT
    GAAGACACAG TGAAGCTTAG ACTTTACAGA TTTACTGAAA GAGATACCTT CATAGAAACC
    TTTATCCTGT GGGTCTATCT CCTGGAACCA GACTGTAACA TCATCCATAT GAGTAACAAT
    GTGCTGGAGG TGCCTGAATT CAATGGCCTG TCCCAAGCGA TTGATAAAAA TCTGCTCAGA
    TTCGATTATG ATAGGATGGC TAGCCTGGAA TGTACGGTCA GCCTGGACAC TGCGAGAACT
    CGGCTGCCAG CCCATGGCCA GATGGTTCTC GGGGAGCCTC GACCAGAAGA ACCTCGTGGA
    GATCAGCCAC ACAGTTTTTT CCCAGAGTCC CGTATGATGT CTTTTAATGC CTTTCTGGAA
    CTGAGAGCAA AACTGAAGTG TCCAGGTGGA AGCTGTACCC CAGGATTAAA GAAAATAGGA
    AGCCTCAAAG TGAGCTGTGA GGAGTTCCTG CTGATGGGCC TTCGTTATCA GCATCTGGAT
    CCCCCTTCAC CCAACATTGA TTATATCTCC ATCCAACTGG ACCTCACAGA TACCAGGAGT
    AAAATTGTGT ACAAGTCAGA GAGTGCGTGG CTGCCTGTCT ATATCAGAGC TGGAATTCCG
    AATCAGATTC CAAAGGCTGC ATTCATGGCC ATGTTTATTC TGGAAGTGGA TCAGTTCATC
    CTGACCTCCT TGACTACATC AGTTCTGGAC TGTGAAGAAG ATGAGACCCC TAAACCCTTG
    CTGGTGTTCA ACATTACTAA AGCCCCGCTC CAGGGCTATG TGACTCACCT GTTGGATCAC
    ACCAGACCAA TCTCCTCATT CACCTGGAAA GATCTCAGTG ACATGCAGAT CGCCTATCAG
    CCACCAAACA GCAGCCATTC TGAGAGGAGA CATGATGAGG TAGAATTGGA GGTATACGAC
    TTCTTCTTTG AAAGGAGTGC ACCTATGACA GTCCACATCT CCATCAGAAC AGCAGATACA
    AATGCCCCCC GTGTATCCTG GAATACAGGT CTGAATCTCC TTGAGGGGCA GTCTCGAGCC
    ATCACTTGGG AACAGTTTCA GGTTGTCGAC AATGACGACA TCGGTGCTGT CCGGCTAGTC
    ACCGTTGGTG GCCTGCAGCA TGGATGGCTG ACTTTAAGAG GGGGGAAAGG ATTTCTCTTC
    ACCGTGGCTG ACCTCCAGGC TGGAGTTGTT CGCTATCATC ATGATGACAG CGACTCCACC
    AAAGACTTCG TGGTCTTCCG GATATTTGAT GGCCATCACA GCATCCGTCA CAAATTCCCC
    ATCAACGTCT TGCCCAAAGA TGATAGTCCC CCGTTCCTCA TAACCAATGT TGTCATTGAA
    CTGGAGGAGG GGCAGACCAT CCTGATCCAG GGATCCATGC TGCGAGCTTC AGATGTGGAT
    GCCAGTGATG ACTACATCTT CTTCAATATC ACAAAGCCTC CACAGGCTGG GGAGATCATG
    AAGAAGCCAG GGCCAGGACT GATAGGCTAT CCTGTCCCTG GCTTCCTTCA GAGGGATTTG
    TTTAATGGAA TCATTTATTA TCGTCATTTT GGTGGAGAAA TCTTTGAAGA TTCTTTTCAA
    TTTGTCCTGT GGGACAGCCA TGAACCTCCA AATCTTTCAG TGCCACAGGT GGCAACAATC
    CATGTAACTC CAGTGGATGA CCAGCTTCCA AAAGAGGCTC CTGGAGTTTC TCGGCATTTG
    GTTGTCAAGG AAACTGAGGT GGCCTATATA ACTAAGAAAC AGCTACATTT TATAGATTCA
    GAATCATATG ACAGGGAGCT GGTCTACACG ATAACTACTC CTCCATTTTT CTCCTTCAGC
    CACAGACACT TGGATGCTGG GAAATTATTT ATGGTGGACA GCATACCAAA AGTAGTTAAG
    AATCCTACGG CCCTGGAGCT GAGGTCATTC ACTCAGCATG CTGTGAACTA TATGAAAGTG
    GCCTACATGC CCCCCATGCA AGACATTGGT CCCCATTGCA GAGATGTCCA GTTCACATTT
    TCTGTCAGTA ACCAACATGG CGGCACTTTG CATGGGATCT GCTTTAACAT TACAATCCTC
    CCAGTGGACA ATCAAGTTCC TGAGGCGTTC ACCAACCCTC TGAAAGTGAC TGAGGGAGGT
    CAAAGCATCA TCAGCACAGA GCACATTCTG ATTTCTGATG CAGATACCAA GCTGGACAAT
    ATTGACCTCT CCCTGCGGGA ATTGCCTCTG CACGGAAGGG TGGAGCTGAA TGGATTTCCT
    CTAAATTCAG GGGGCACATT TTCTTGGGGC GATCTCCATA CCTTAAAAGT TAGGTATCAA
    CATGATGGAA CTGAAGTTCT TCAGGATGAC CTACTCTTGG AGGTCACCGA TGGCACAAAT
    TCAGCAGAAT TTGTTCTACA TGTTGAGGTA TTCCCTGTCA ACGATGAGCC ACCAGTCTTA
    AAGGCTGACC TCATGCCTGT CATGAATTGC TCAGAGGGAG GAGAGGTGGT CATCACCTCT
    GAATACATTT TTGCTACTGA TGTGGACAGC GATAACTTGA AGTTGATGTT TGTGATTGCT
    CGCGAACCTC AGCATGGGGT GGTGAGGAGA GCTGGAGTCA CAGTGGATCA GTTCTCTCAG
    AGAGATGTTA TCTCAGAGGC CGTGACATAC AAACACACAG GTGGCGAGAT TGGCATGATG
    CCCTGTTTTG ACACCATTAC ATTGGTGGTT TCGGATGGAG AGGCTGGCCC TTTTGTGAAT
    GGCTGTTGCT ACAATGGACC CAATCCATCT GTTCCGCTTC ATGCGTCCTT TCCAGTGTAT
    GATCTCAACA TCACGGTATA CCCAGTAGAC AACCAGCCAC CTTCCATTGC AATAGGTCCC
    ATGTTTGTTG TAGATGAGGG CTGCTCAACA GCCCTTACTG TTAACCATTT GTCCGCCACT
    GACCCTGACA CTGCAGCAGA TGACTTGGAA TTTGTTTTGG TTTCTCCTCC TCAGTTTGGC
    TACCTCGAAA ATATACTCCC TTCTGTGGGT TTTGAAAAAA GCAATATTGG CATAAGTATA
    GATTCATTTC AGTGGAAAGA CATGAACGCT TTTCACATTA ACTATGTGCA GTCCAGGCAT
    CTGAGGATAG AACCAACTGC CGACCAGTTC ACGGTGTACG TCACAGATGG GAAGCAACAC
    TCCTTGGAGA TACCATTTTC TATTATAATC AACCCCACAA ATGATGAAGC TCCTGACTTT
    GTAGTGCAGA ATATTACTGT GTGCGAGGGT CAGATGAAAG AGCTGGACTC TTCCATCATC
    AGCGCTGTGG ACCTGGACAT TCCCCAGGAT GCCCTGCTGT TCAGCATCAC TCAAAAGCCA
    CGCCATGGCC TCCTCATCGA TAGGGGGTTT AGCAAAGACT TCTCTGAGAA TAAGCAGCCA
    GCCAACCCTC ACCAGAAACA TGTACCTGTT CACAGTTTTT CCATGGAACT CCTCAAGACT
    GGAATGAGGT TGACGTACAT GCATGATGAC TCAGAGAGCC TTGCTGATGA TTTTACAATC
    CGTTTGTCAG ATGGGAAACA TAAGATACTT AAAACCATTT CAATAGAGGT CATCCCAGTT
    AATGATGAAA AACCAATGCT GAGCAAGAAG GCTGAAATTG CAATGAATAT GGGTGAAACT
    CGTATTATTT CCAGTGCTAT TCTTTCAGCC ATAGATGAAG ACTCACCCAG GGAGAAGATT
    TACTATGTAT TTGAAAGGCT TCCCCAAAAT GGGCAACTTC AGCTTAAGAT AGGGAGGGAC
    TGGGTTCCTC TCTCCCCTGG CATGAAATGC ACTCAGGAGG AAGTGGATCT GAACTTGCTG
    AGATACACAC ACACCGGGGC AATGGATTCC CAGAATCAAG ATAGCTTCAC CTTCTACCTT
    TGGGATGGCA ACAACAGGTC CCCTGCTCTT GACTGTCAAA TCACCATCAA GGACATGGAA
    AAAGGTGATA TTGTCATCCT TACAAAACCA CTCGTGGTGT CTAAAGGTGA TAGAGGTTTC
    TTAACAACCA CCACTCTCCT GGCTGTGGAC GGAACAGACA AGCCTGAGGA ACTGCTCTAT
    GTCATCACCT CCCCTCCACG ATATGGCCAG ATCGAATATG TTCACTATCC TGGAGTTCCC
    ATTACAAACT TCAGCCAAAT GGATATAGCG GGGCAGACAG TTTGCTATGT ACACAAGAGC
    AAGGTGACTG TCTCCAGTGA CAGATTCAGA TTCATCATCA GCAATGGACT GCGGACCGAG
    CACGGGGTGT TTGAGATCAC ACTGGAGACT GTGGACAGAG CCCTGCCTGT GGTAACCAGG
    AACAAGGGGT TGAGACTGGC CCAAGGGGCC GTGGGCCTGC TTTCCCCTGA CCTCCTTCAG
    CTGACCGACC CTGATACACC TGTGGAGAAC CTCACCTTCC TCTTGGTTCA GCTTCCCCAG
    CATGGCCAGC TCTACCTGTG GGGGACAGGG CTACTTCAAC ACAATTTCAC CCAGCAGGAT
    GTGGACAGCA GGAATGTGGC CTATCGGCAC TCAGGAGGGG ACTCCCAGAC TGACTGCTTT
    ACTTTCATGG CCACAGACGG GACAAACCAA GGCTTTATTG TGAATGGGAG AGTATGGGAA
    GAACCTGTTT TATTCACCAT TCAGGTAGAC CAATTGGACA AAACAGCTCC TCGTATCACA
    CTCTTGCATT CCCCTTCTCA AGTGGGGCTC CTGAAAAATG GCTGCTACGG GATTTACATC
    ACTTCCCGCG TGTTGAAGGC ATCAGACCCT GACACTGAGG ACGATCAGAT CATCTTTAAA
    ATTCTACAAG GCCCAAAACA TGGACATCTG GAGAACACAA CAACAGGTGA ATTTATCCAT
    GAGAAATTTA GCCAAAAGGA CTTAAACAGT AAGACTATTC TTTACATCAT AAACCCATCT
    TTGGAAGTAA ATTCAGATAC CGTGGAATTT CAAATCATGG ACCCCACAGG GAACTCGGCC
    ACTCCTCAAA TTTTGGAACT GAAGTGGTCT CATATTGAAT GGTCACAGAC CGAATATGAA
    GTCTGTGAGA ATGTGGGTGT GTTGCCCTTG GAAATTATCA GAAGGGGATA TTCCATGGAC
    TCGGCCTTTG TGGGTATAAA GGTCAACCAA GTGTCAGCTG CAGATGGAAA AGATTTCACC
    GTGATTCCAT CTAAACTGAT TCAGTTTGAC CCAGGAATGT CAACTAAGAT GTGGAATATA
    GCAATTACCT ATGACGGATT AGAGGAAGAT GATGAGGTCT TTGAAGTAAT TCTGAACTCC
    CCTGTGAATG CAGTTCTTGG CACAAAGACA AAAGCTGCAG TGAAAATTTT GGACTCAAAA
    GGAGGACAAT GCCATCCTTC ATATTCCTCC AACCAAAGCA AGCACAGCAC ATGGGAGAAG
    GGCATTTGGC ATCTGCTGCC CCCAGGGTCT TCCTCATCCA CCACTTCTGG TTCCTTTCAT
    CTGGAAAGAA GACCTCTTCC ATCTTCCATG CAGCTAGCAG TCACCAGGGG AGACACCCTG
    CGGGGCTTTG ATTCTACAGA TCTTTCTCAA AGGAAGCTTA GGACCCGTGG GAATGGCAAA
    ACAGTTCGTC CATCCTCTGT TTATAGAAAT GGAACAGACA TCATCTATAA TTATCATGGG
    ATAGTTTCCT TGAAACTAGA GGATGACAGC TTCCCAACTC ACAAAAGGAA GGCCAAAGTA
    TCCATCATTA GTCAGCCACA AAAGACAATC AAAGTGGCAG AACTGCCTCA AGCAGATAAG
    GTGGAATCCA CAACTGACTC ACACTTCCCC AGACAGGACC AGTTGCCCTC ATTTCCAAAG
    AACTGCACTC TGGAATTAAA GGGACTCTTC CATTTTGAAG AAGGCATCCA GAAGCTGTAT
    CAGTGCAATG GGATTGCCTG GAAAGCCTGG AGTCCCCAAA CCAAGGATGT GGAAGAAAAA
    TCCTGTCCAG CCGGGTGGCA CCAGCACTCA GGCTACTGTC ACATCTTGAT CACAGAGCAG
    AAAGGCACCT GGAATGCGGC TGCCCAAGCT TGCAGGGAAC AATACCTGGG CAACCTTGTA
    ACTGTATTCT CCAGGCAGCA CATGCGGTGG CTCTGGGACA TTGGTGAGAG AAAGTCCTTT
    TGGATAGGTT TGAACGACCA AGTGCATGCT GGCCACTGGG AGTGGATCGG TGGTGAACCT
    GTTGCCTTCA CCAATGGGAG AAGAGGGCCC TCTCAACGCT CCAAGCTTGG AAAGAGCTGT
    GTTTTGGTTC AAAGACAAGG GAAATGGCAA ACAAAAGATT GTAGGAGAGC CAAACCTCAT
    AATTATGTGT GTTCCAGAAA ACTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OPc109241
    Clone ID Related Accession (Same CDS sequence) XM_024345565.1 , XM_024345568.1 , XM_024345567.1 , XM_024345569.1 , XM_024345566.1
    Accession Version XM_024345568.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6567bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-03-19
    Organism Pan troglodytes(chimpanzee)
    Product FRAS1-related extracellular matrix protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036888.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    ATGAACTCTC TGAGTTGGGG GGCTGCGAAT GCCGTGCTGC TGCTGCTCCT CCTGGCCTGG 
    GCCAGCCCCA CCTTCATCAG CATCAACCGT GGGGTGAGGG TGATGAAGGG CCACTCTGCC
    TTCCTGTCAG GAGATGACCT GAAGTTTGCC ATCCCTAAAG AGAAAGATGC CTGCAAAGTG
    GAAGTTGTGA TGAATGAGCC AATAACCCAG AGGGTTGGGA AACTCACTCC ACAGGTCTTT
    GACTGCCATT TCCTTCCCAA TGAAGTCAAG TATGTTCACA ATGGTTGTCC AATTCTTGAT
    GAAGACACAG TGAAGCTTAG ACTTTACAGA TTTACTGAAA GAGATACCTT CATAGAAACC
    TTTATCCTGT GGGTCTATCT CCTGGAACCA GACTGTAACA TCATCCATAT GAGTAACAAT
    GTGCTGGAGG TGCCTGAATT CAATGGCCTG TCCCAAGCGA TTGATAAAAA TCTGCTCAGA
    TTCGATTATG ATAGGATGGC TAGCCTGGAA TGTACGGTCA GCCTGGACAC TGCGAGAACT
    CGGCTGCCAG CCCATGGCCA GATGGTTCTC GGGGAGCCTC GACCAGAAGA ACCTCGTGGA
    GATCAGCCAC ACAGTTTTTT CCCAGAGTCC CGTATGATGT CTTTTAATGC CTTTCTGGAA
    CTGAGAGCAA AACTGAAGTG TCCAGGTGGA AGCTGTACCC CAGGATTAAA GAAAATAGGA
    AGCCTCAAAG TGAGCTGTGA GGAGTTCCTG CTGATGGGCC TTCGTTATCA GCATCTGGAT
    CCCCCTTCAC CCAACATTGA TTATATCTCC ATCCAACTGG ACCTCACAGA TACCAGGAGT
    AAAATTGTGT ACAAGTCAGA GAGTGCGTGG CTGCCTGTCT ATATCAGAGC TGGAATTCCG
    AATCAGATTC CAAAGGCTGC ATTCATGGCC ATGTTTATTC TGGAAGTGGA TCAGTTCATC
    CTGACCTCCT TGACTACATC AGTTCTGGAC TGTGAAGAAG ATGAGACCCC TAAACCCTTG
    CTGGTGTTCA ACATTACTAA AGCCCCGCTC CAGGGCTATG TGACTCACCT GTTGGATCAC
    ACCAGACCAA TCTCCTCATT CACCTGGAAA GATCTCAGTG ACATGCAGAT CGCCTATCAG
    CCACCAAACA GCAGCCATTC TGAGAGGAGA CATGATGAGG TAGAATTGGA GGTATACGAC
    TTCTTCTTTG AAAGGAGTGC ACCTATGACA GTCCACATCT CCATCAGAAC AGCAGATACA
    AATGCCCCCC GTGTATCCTG GAATACAGGT CTGAATCTCC TTGAGGGGCA GTCTCGAGCC
    ATCACTTGGG AACAGTTTCA GGTTGTCGAC AATGACGACA TCGGTGCTGT CCGGCTAGTC
    ACCGTTGGTG GCCTGCAGCA TGGATGGCTG ACTTTAAGAG GGGGGAAAGG ATTTCTCTTC
    ACCGTGGCTG ACCTCCAGGC TGGAGTTGTT CGCTATCATC ATGATGACAG CGACTCCACC
    AAAGACTTCG TGGTCTTCCG GATATTTGAT GGCCATCACA GCATCCGTCA CAAATTCCCC
    ATCAACGTCT TGCCCAAAGA TGATAGTCCC CCGTTCCTCA TAACCAATGT TGTCATTGAA
    CTGGAGGAGG GGCAGACCAT CCTGATCCAG GGATCCATGC TGCGAGCTTC AGATGTGGAT
    GCCAGTGATG ACTACATCTT CTTCAATATC ACAAAGCCTC CACAGGCTGG GGAGATCATG
    AAGAAGCCAG GGCCAGGACT GATAGGCTAT CCTGTCCCTG GCTTCCTTCA GAGGGATTTG
    TTTAATGGAA TCATTTATTA TCGTCATTTT GGTGGAGAAA TCTTTGAAGA TTCTTTTCAA
    TTTGTCCTGT GGGACAGCCA TGAACCTCCA AATCTTTCAG TGCCACAGGT GGCAACAATC
    CATGTAACTC CAGTGGATGA CCAGCTTCCA AAAGAGGCTC CTGGAGTTTC TCGGCATTTG
    GTTGTCAAGG AAACTGAGGT GGCCTATATA ACTAAGAAAC AGCTACATTT TATAGATTCA
    GAATCATATG ACAGGGAGCT GGTCTACACG ATAACTACTC CTCCATTTTT CTCCTTCAGC
    CACAGACACT TGGATGCTGG GAAATTATTT ATGGTGGACA GCATACCAAA AGTAGTTAAG
    AATCCTACGG CCCTGGAGCT GAGGTCATTC ACTCAGCATG CTGTGAACTA TATGAAAGTG
    GCCTACATGC CCCCCATGCA AGACATTGGT CCCCATTGCA GAGATGTCCA GTTCACATTT
    TCTGTCAGTA ACCAACATGG CGGCACTTTG CATGGGATCT GCTTTAACAT TACAATCCTC
    CCAGTGGACA ATCAAGTTCC TGAGGCGTTC ACCAACCCTC TGAAAGTGAC TGAGGGAGGT
    CAAAGCATCA TCAGCACAGA GCACATTCTG ATTTCTGATG CAGATACCAA GCTGGACAAT
    ATTGACCTCT CCCTGCGGGA ATTGCCTCTG CACGGAAGGG TGGAGCTGAA TGGATTTCCT
    CTAAATTCAG GGGGCACATT TTCTTGGGGC GATCTCCATA CCTTAAAAGT TAGGTATCAA
    CATGATGGAA CTGAAGTTCT TCAGGATGAC CTACTCTTGG AGGTCACCGA TGGCACAAAT
    TCAGCAGAAT TTGTTCTACA TGTTGAGGTA TTCCCTGTCA ACGATGAGCC ACCAGTCTTA
    AAGGCTGACC TCATGCCTGT CATGAATTGC TCAGAGGGAG GAGAGGTGGT CATCACCTCT
    GAATACATTT TTGCTACTGA TGTGGACAGC GATAACTTGA AGTTGATGTT TGTGATTGCT
    CGCGAACCTC AGCATGGGGT GGTGAGGAGA GCTGGAGTCA CAGTGGATCA GTTCTCTCAG
    AGAGATGTTA TCTCAGAGGC CGTGACATAC AAACACACAG GTGGCGAGAT TGGCATGATG
    CCCTGTTTTG ACACCATTAC ATTGGTGGTT TCGGATGGAG AGGCTGGCCC TTTTGTGAAT
    GGCTGTTGCT ACAATGGACC CAATCCATCT GTTCCGCTTC ATGCGTCCTT TCCAGTGTAT
    GATCTCAACA TCACGGTATA CCCAGTAGAC AACCAGCCAC CTTCCATTGC AATAGGTCCC
    ATGTTTGTTG TAGATGAGGG CTGCTCAACA GCCCTTACTG TTAACCATTT GTCCGCCACT
    GACCCTGACA CTGCAGCAGA TGACTTGGAA TTTGTTTTGG TTTCTCCTCC TCAGTTTGGC
    TACCTCGAAA ATATACTCCC TTCTGTGGGT TTTGAAAAAA GCAATATTGG CATAAGTATA
    GATTCATTTC AGTGGAAAGA CATGAACGCT TTTCACATTA ACTATGTGCA GTCCAGGCAT
    CTGAGGATAG AACCAACTGC CGACCAGTTC ACGGTGTACG TCACAGATGG GAAGCAACAC
    TCCTTGGAGA TACCATTTTC TATTATAATC AACCCCACAA ATGATGAAGC TCCTGACTTT
    GTAGTGCAGA ATATTACTGT GTGCGAGGGT CAGATGAAAG AGCTGGACTC TTCCATCATC
    AGCGCTGTGG ACCTGGACAT TCCCCAGGAT GCCCTGCTGT TCAGCATCAC TCAAAAGCCA
    CGCCATGGCC TCCTCATCGA TAGGGGGTTT AGCAAAGACT TCTCTGAGAA TAAGCAGCCA
    GCCAACCCTC ACCAGAAACA TGTACCTGTT CACAGTTTTT CCATGGAACT CCTCAAGACT
    GGAATGAGGT TGACGTACAT GCATGATGAC TCAGAGAGCC TTGCTGATGA TTTTACAATC
    CGTTTGTCAG ATGGGAAACA TAAGATACTT AAAACCATTT CAATAGAGGT CATCCCAGTT
    AATGATGAAA AACCAATGCT GAGCAAGAAG GCTGAAATTG CAATGAATAT GGGTGAAACT
    CGTATTATTT CCAGTGCTAT TCTTTCAGCC ATAGATGAAG ACTCACCCAG GGAGAAGATT
    TACTATGTAT TTGAAAGGCT TCCCCAAAAT GGGCAACTTC AGCTTAAGAT AGGGAGGGAC
    TGGGTTCCTC TCTCCCCTGG CATGAAATGC ACTCAGGAGG AAGTGGATCT GAACTTGCTG
    AGATACACAC ACACCGGGGC AATGGATTCC CAGAATCAAG ATAGCTTCAC CTTCTACCTT
    TGGGATGGCA ACAACAGGTC CCCTGCTCTT GACTGTCAAA TCACCATCAA GGACATGGAA
    AAAGGTGATA TTGTCATCCT TACAAAACCA CTCGTGGTGT CTAAAGGTGA TAGAGGTTTC
    TTAACAACCA CCACTCTCCT GGCTGTGGAC GGAACAGACA AGCCTGAGGA ACTGCTCTAT
    GTCATCACCT CCCCTCCACG ATATGGCCAG ATCGAATATG TTCACTATCC TGGAGTTCCC
    ATTACAAACT TCAGCCAAAT GGATATAGCG GGGCAGACAG TTTGCTATGT ACACAAGAGC
    AAGGTGACTG TCTCCAGTGA CAGATTCAGA TTCATCATCA GCAATGGACT GCGGACCGAG
    CACGGGGTGT TTGAGATCAC ACTGGAGACT GTGGACAGAG CCCTGCCTGT GGTAACCAGG
    AACAAGGGGT TGAGACTGGC CCAAGGGGCC GTGGGCCTGC TTTCCCCTGA CCTCCTTCAG
    CTGACCGACC CTGATACACC TGTGGAGAAC CTCACCTTCC TCTTGGTTCA GCTTCCCCAG
    CATGGCCAGC TCTACCTGTG GGGGACAGGG CTACTTCAAC ACAATTTCAC CCAGCAGGAT
    GTGGACAGCA GGAATGTGGC CTATCGGCAC TCAGGAGGGG ACTCCCAGAC TGACTGCTTT
    ACTTTCATGG CCACAGACGG GACAAACCAA GGCTTTATTG TGAATGGGAG AGTATGGGAA
    GAACCTGTTT TATTCACCAT TCAGGTAGAC CAATTGGACA AAACAGCTCC TCGTATCACA
    CTCTTGCATT CCCCTTCTCA AGTGGGGCTC CTGAAAAATG GCTGCTACGG GATTTACATC
    ACTTCCCGCG TGTTGAAGGC ATCAGACCCT GACACTGAGG ACGATCAGAT CATCTTTAAA
    ATTCTACAAG GCCCAAAACA TGGACATCTG GAGAACACAA CAACAGGTGA ATTTATCCAT
    GAGAAATTTA GCCAAAAGGA CTTAAACAGT AAGACTATTC TTTACATCAT AAACCCATCT
    TTGGAAGTAA ATTCAGATAC CGTGGAATTT CAAATCATGG ACCCCACAGG GAACTCGGCC
    ACTCCTCAAA TTTTGGAACT GAAGTGGTCT CATATTGAAT GGTCACAGAC CGAATATGAA
    GTCTGTGAGA ATGTGGGTGT GTTGCCCTTG GAAATTATCA GAAGGGGATA TTCCATGGAC
    TCGGCCTTTG TGGGTATAAA GGTCAACCAA GTGTCAGCTG CAGATGGAAA AGATTTCACC
    GTGATTCCAT CTAAACTGAT TCAGTTTGAC CCAGGAATGT CAACTAAGAT GTGGAATATA
    GCAATTACCT ATGACGGATT AGAGGAAGAT GATGAGGTCT TTGAAGTAAT TCTGAACTCC
    CCTGTGAATG CAGTTCTTGG CACAAAGACA AAAGCTGCAG TGAAAATTTT GGACTCAAAA
    GGAGGACAAT GCCATCCTTC ATATTCCTCC AACCAAAGCA AGCACAGCAC ATGGGAGAAG
    GGCATTTGGC ATCTGCTGCC CCCAGGGTCT TCCTCATCCA CCACTTCTGG TTCCTTTCAT
    CTGGAAAGAA GACCTCTTCC ATCTTCCATG CAGCTAGCAG TCACCAGGGG AGACACCCTG
    CGGGGCTTTG ATTCTACAGA TCTTTCTCAA AGGAAGCTTA GGACCCGTGG GAATGGCAAA
    ACAGTTCGTC CATCCTCTGT TTATAGAAAT GGAACAGACA TCATCTATAA TTATCATGGG
    ATAGTTTCCT TGAAACTAGA GGATGACAGC TTCCCAACTC ACAAAAGGAA GGCCAAAGTA
    TCCATCATTA GTCAGCCACA AAAGACAATC AAAGTGGCAG AACTGCCTCA AGCAGATAAG
    GTGGAATCCA CAACTGACTC ACACTTCCCC AGACAGGACC AGTTGCCCTC ATTTCCAAAG
    AACTGCACTC TGGAATTAAA GGGACTCTTC CATTTTGAAG AAGGCATCCA GAAGCTGTAT
    CAGTGCAATG GGATTGCCTG GAAAGCCTGG AGTCCCCAAA CCAAGGATGT GGAAGAAAAA
    TCCTGTCCAG CCGGGTGGCA CCAGCACTCA GGCTACTGTC ACATCTTGAT CACAGAGCAG
    AAAGGCACCT GGAATGCGGC TGCCCAAGCT TGCAGGGAAC AATACCTGGG CAACCTTGTA
    ACTGTATTCT CCAGGCAGCA CATGCGGTGG CTCTGGGACA TTGGTGAGAG AAAGTCCTTT
    TGGATAGGTT TGAACGACCA AGTGCATGCT GGCCACTGGG AGTGGATCGG TGGTGAACCT
    GTTGCCTTCA CCAATGGGAG AAGAGGGCCC TCTCAACGCT CCAAGCTTGG AAAGAGCTGT
    GTTTTGGTTC AAAGACAAGG GAAATGGCAA ACAAAAGATT GTAGGAGAGC CAAACCTCAT
    AATTATGTGT GTTCCAGAAA ACTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024201336.1
    CDS1062..7628
    Translation

    Target ORF information:

    RefSeq Version XM_024345568.1
    Organism Pan troglodytes(chimpanzee)
    Definition Pan troglodytes FRAS1 related extracellular matrix 1 (FREM1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024345568.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    ATGAACTCTC TGAGTTGGGG GGCTGCGAAT GCCGTGCTGC TGCTGCTCCT CCTGGCCTGG 
    GCCAGCCCCA CCTTCATCAG CATCAACCGT GGGGTGAGGG TGATGAAGGG CCACTCTGCC
    TTCCTGTCAG GAGATGACCT GAAGTTTGCC ATCCCTAAAG AGAAAGATGC CTGCAAAGTG
    GAAGTTGTGA TGAATGAGCC AATAACCCAG AGGGTTGGGA AACTCACTCC ACAGGTCTTT
    GACTGCCATT TCCTTCCCAA TGAAGTCAAG TATGTTCACA ATGGTTGTCC AATTCTTGAT
    GAAGACACAG TGAAGCTTAG ACTTTACAGA TTTACTGAAA GAGATACCTT CATAGAAACC
    TTTATCCTGT GGGTCTATCT CCTGGAACCA GACTGTAACA TCATCCATAT GAGTAACAAT
    GTGCTGGAGG TGCCTGAATT CAATGGCCTG TCCCAAGCGA TTGATAAAAA TCTGCTCAGA
    TTCGATTATG ATAGGATGGC TAGCCTGGAA TGTACGGTCA GCCTGGACAC TGCGAGAACT
    CGGCTGCCAG CCCATGGCCA GATGGTTCTC GGGGAGCCTC GACCAGAAGA ACCTCGTGGA
    GATCAGCCAC ACAGTTTTTT CCCAGAGTCC CGTATGATGT CTTTTAATGC CTTTCTGGAA
    CTGAGAGCAA AACTGAAGTG TCCAGGTGGA AGCTGTACCC CAGGATTAAA GAAAATAGGA
    AGCCTCAAAG TGAGCTGTGA GGAGTTCCTG CTGATGGGCC TTCGTTATCA GCATCTGGAT
    CCCCCTTCAC CCAACATTGA TTATATCTCC ATCCAACTGG ACCTCACAGA TACCAGGAGT
    AAAATTGTGT ACAAGTCAGA GAGTGCGTGG CTGCCTGTCT ATATCAGAGC TGGAATTCCG
    AATCAGATTC CAAAGGCTGC ATTCATGGCC ATGTTTATTC TGGAAGTGGA TCAGTTCATC
    CTGACCTCCT TGACTACATC AGTTCTGGAC TGTGAAGAAG ATGAGACCCC TAAACCCTTG
    CTGGTGTTCA ACATTACTAA AGCCCCGCTC CAGGGCTATG TGACTCACCT GTTGGATCAC
    ACCAGACCAA TCTCCTCATT CACCTGGAAA GATCTCAGTG ACATGCAGAT CGCCTATCAG
    CCACCAAACA GCAGCCATTC TGAGAGGAGA CATGATGAGG TAGAATTGGA GGTATACGAC
    TTCTTCTTTG AAAGGAGTGC ACCTATGACA GTCCACATCT CCATCAGAAC AGCAGATACA
    AATGCCCCCC GTGTATCCTG GAATACAGGT CTGAATCTCC TTGAGGGGCA GTCTCGAGCC
    ATCACTTGGG AACAGTTTCA GGTTGTCGAC AATGACGACA TCGGTGCTGT CCGGCTAGTC
    ACCGTTGGTG GCCTGCAGCA TGGATGGCTG ACTTTAAGAG GGGGGAAAGG ATTTCTCTTC
    ACCGTGGCTG ACCTCCAGGC TGGAGTTGTT CGCTATCATC ATGATGACAG CGACTCCACC
    AAAGACTTCG TGGTCTTCCG GATATTTGAT GGCCATCACA GCATCCGTCA CAAATTCCCC
    ATCAACGTCT TGCCCAAAGA TGATAGTCCC CCGTTCCTCA TAACCAATGT TGTCATTGAA
    CTGGAGGAGG GGCAGACCAT CCTGATCCAG GGATCCATGC TGCGAGCTTC AGATGTGGAT
    GCCAGTGATG ACTACATCTT CTTCAATATC ACAAAGCCTC CACAGGCTGG GGAGATCATG
    AAGAAGCCAG GGCCAGGACT GATAGGCTAT CCTGTCCCTG GCTTCCTTCA GAGGGATTTG
    TTTAATGGAA TCATTTATTA TCGTCATTTT GGTGGAGAAA TCTTTGAAGA TTCTTTTCAA
    TTTGTCCTGT GGGACAGCCA TGAACCTCCA AATCTTTCAG TGCCACAGGT GGCAACAATC
    CATGTAACTC CAGTGGATGA CCAGCTTCCA AAAGAGGCTC CTGGAGTTTC TCGGCATTTG
    GTTGTCAAGG AAACTGAGGT GGCCTATATA ACTAAGAAAC AGCTACATTT TATAGATTCA
    GAATCATATG ACAGGGAGCT GGTCTACACG ATAACTACTC CTCCATTTTT CTCCTTCAGC
    CACAGACACT TGGATGCTGG GAAATTATTT ATGGTGGACA GCATACCAAA AGTAGTTAAG
    AATCCTACGG CCCTGGAGCT GAGGTCATTC ACTCAGCATG CTGTGAACTA TATGAAAGTG
    GCCTACATGC CCCCCATGCA AGACATTGGT CCCCATTGCA GAGATGTCCA GTTCACATTT
    TCTGTCAGTA ACCAACATGG CGGCACTTTG CATGGGATCT GCTTTAACAT TACAATCCTC
    CCAGTGGACA ATCAAGTTCC TGAGGCGTTC ACCAACCCTC TGAAAGTGAC TGAGGGAGGT
    CAAAGCATCA TCAGCACAGA GCACATTCTG ATTTCTGATG CAGATACCAA GCTGGACAAT
    ATTGACCTCT CCCTGCGGGA ATTGCCTCTG CACGGAAGGG TGGAGCTGAA TGGATTTCCT
    CTAAATTCAG GGGGCACATT TTCTTGGGGC GATCTCCATA CCTTAAAAGT TAGGTATCAA
    CATGATGGAA CTGAAGTTCT TCAGGATGAC CTACTCTTGG AGGTCACCGA TGGCACAAAT
    TCAGCAGAAT TTGTTCTACA TGTTGAGGTA TTCCCTGTCA ACGATGAGCC ACCAGTCTTA
    AAGGCTGACC TCATGCCTGT CATGAATTGC TCAGAGGGAG GAGAGGTGGT CATCACCTCT
    GAATACATTT TTGCTACTGA TGTGGACAGC GATAACTTGA AGTTGATGTT TGTGATTGCT
    CGCGAACCTC AGCATGGGGT GGTGAGGAGA GCTGGAGTCA CAGTGGATCA GTTCTCTCAG
    AGAGATGTTA TCTCAGAGGC CGTGACATAC AAACACACAG GTGGCGAGAT TGGCATGATG
    CCCTGTTTTG ACACCATTAC ATTGGTGGTT TCGGATGGAG AGGCTGGCCC TTTTGTGAAT
    GGCTGTTGCT ACAATGGACC CAATCCATCT GTTCCGCTTC ATGCGTCCTT TCCAGTGTAT
    GATCTCAACA TCACGGTATA CCCAGTAGAC AACCAGCCAC CTTCCATTGC AATAGGTCCC
    ATGTTTGTTG TAGATGAGGG CTGCTCAACA GCCCTTACTG TTAACCATTT GTCCGCCACT
    GACCCTGACA CTGCAGCAGA TGACTTGGAA TTTGTTTTGG TTTCTCCTCC TCAGTTTGGC
    TACCTCGAAA ATATACTCCC TTCTGTGGGT TTTGAAAAAA GCAATATTGG CATAAGTATA
    GATTCATTTC AGTGGAAAGA CATGAACGCT TTTCACATTA ACTATGTGCA GTCCAGGCAT
    CTGAGGATAG AACCAACTGC CGACCAGTTC ACGGTGTACG TCACAGATGG GAAGCAACAC
    TCCTTGGAGA TACCATTTTC TATTATAATC AACCCCACAA ATGATGAAGC TCCTGACTTT
    GTAGTGCAGA ATATTACTGT GTGCGAGGGT CAGATGAAAG AGCTGGACTC TTCCATCATC
    AGCGCTGTGG ACCTGGACAT TCCCCAGGAT GCCCTGCTGT TCAGCATCAC TCAAAAGCCA
    CGCCATGGCC TCCTCATCGA TAGGGGGTTT AGCAAAGACT TCTCTGAGAA TAAGCAGCCA
    GCCAACCCTC ACCAGAAACA TGTACCTGTT CACAGTTTTT CCATGGAACT CCTCAAGACT
    GGAATGAGGT TGACGTACAT GCATGATGAC TCAGAGAGCC TTGCTGATGA TTTTACAATC
    CGTTTGTCAG ATGGGAAACA TAAGATACTT AAAACCATTT CAATAGAGGT CATCCCAGTT
    AATGATGAAA AACCAATGCT GAGCAAGAAG GCTGAAATTG CAATGAATAT GGGTGAAACT
    CGTATTATTT CCAGTGCTAT TCTTTCAGCC ATAGATGAAG ACTCACCCAG GGAGAAGATT
    TACTATGTAT TTGAAAGGCT TCCCCAAAAT GGGCAACTTC AGCTTAAGAT AGGGAGGGAC
    TGGGTTCCTC TCTCCCCTGG CATGAAATGC ACTCAGGAGG AAGTGGATCT GAACTTGCTG
    AGATACACAC ACACCGGGGC AATGGATTCC CAGAATCAAG ATAGCTTCAC CTTCTACCTT
    TGGGATGGCA ACAACAGGTC CCCTGCTCTT GACTGTCAAA TCACCATCAA GGACATGGAA
    AAAGGTGATA TTGTCATCCT TACAAAACCA CTCGTGGTGT CTAAAGGTGA TAGAGGTTTC
    TTAACAACCA CCACTCTCCT GGCTGTGGAC GGAACAGACA AGCCTGAGGA ACTGCTCTAT
    GTCATCACCT CCCCTCCACG ATATGGCCAG ATCGAATATG TTCACTATCC TGGAGTTCCC
    ATTACAAACT TCAGCCAAAT GGATATAGCG GGGCAGACAG TTTGCTATGT ACACAAGAGC
    AAGGTGACTG TCTCCAGTGA CAGATTCAGA TTCATCATCA GCAATGGACT GCGGACCGAG
    CACGGGGTGT TTGAGATCAC ACTGGAGACT GTGGACAGAG CCCTGCCTGT GGTAACCAGG
    AACAAGGGGT TGAGACTGGC CCAAGGGGCC GTGGGCCTGC TTTCCCCTGA CCTCCTTCAG
    CTGACCGACC CTGATACACC TGTGGAGAAC CTCACCTTCC TCTTGGTTCA GCTTCCCCAG
    CATGGCCAGC TCTACCTGTG GGGGACAGGG CTACTTCAAC ACAATTTCAC CCAGCAGGAT
    GTGGACAGCA GGAATGTGGC CTATCGGCAC TCAGGAGGGG ACTCCCAGAC TGACTGCTTT
    ACTTTCATGG CCACAGACGG GACAAACCAA GGCTTTATTG TGAATGGGAG AGTATGGGAA
    GAACCTGTTT TATTCACCAT TCAGGTAGAC CAATTGGACA AAACAGCTCC TCGTATCACA
    CTCTTGCATT CCCCTTCTCA AGTGGGGCTC CTGAAAAATG GCTGCTACGG GATTTACATC
    ACTTCCCGCG TGTTGAAGGC ATCAGACCCT GACACTGAGG ACGATCAGAT CATCTTTAAA
    ATTCTACAAG GCCCAAAACA TGGACATCTG GAGAACACAA CAACAGGTGA ATTTATCCAT
    GAGAAATTTA GCCAAAAGGA CTTAAACAGT AAGACTATTC TTTACATCAT AAACCCATCT
    TTGGAAGTAA ATTCAGATAC CGTGGAATTT CAAATCATGG ACCCCACAGG GAACTCGGCC
    ACTCCTCAAA TTTTGGAACT GAAGTGGTCT CATATTGAAT GGTCACAGAC CGAATATGAA
    GTCTGTGAGA ATGTGGGTGT GTTGCCCTTG GAAATTATCA GAAGGGGATA TTCCATGGAC
    TCGGCCTTTG TGGGTATAAA GGTCAACCAA GTGTCAGCTG CAGATGGAAA AGATTTCACC
    GTGATTCCAT CTAAACTGAT TCAGTTTGAC CCAGGAATGT CAACTAAGAT GTGGAATATA
    GCAATTACCT ATGACGGATT AGAGGAAGAT GATGAGGTCT TTGAAGTAAT TCTGAACTCC
    CCTGTGAATG CAGTTCTTGG CACAAAGACA AAAGCTGCAG TGAAAATTTT GGACTCAAAA
    GGAGGACAAT GCCATCCTTC ATATTCCTCC AACCAAAGCA AGCACAGCAC ATGGGAGAAG
    GGCATTTGGC ATCTGCTGCC CCCAGGGTCT TCCTCATCCA CCACTTCTGG TTCCTTTCAT
    CTGGAAAGAA GACCTCTTCC ATCTTCCATG CAGCTAGCAG TCACCAGGGG AGACACCCTG
    CGGGGCTTTG ATTCTACAGA TCTTTCTCAA AGGAAGCTTA GGACCCGTGG GAATGGCAAA
    ACAGTTCGTC CATCCTCTGT TTATAGAAAT GGAACAGACA TCATCTATAA TTATCATGGG
    ATAGTTTCCT TGAAACTAGA GGATGACAGC TTCCCAACTC ACAAAAGGAA GGCCAAAGTA
    TCCATCATTA GTCAGCCACA AAAGACAATC AAAGTGGCAG AACTGCCTCA AGCAGATAAG
    GTGGAATCCA CAACTGACTC ACACTTCCCC AGACAGGACC AGTTGCCCTC ATTTCCAAAG
    AACTGCACTC TGGAATTAAA GGGACTCTTC CATTTTGAAG AAGGCATCCA GAAGCTGTAT
    CAGTGCAATG GGATTGCCTG GAAAGCCTGG AGTCCCCAAA CCAAGGATGT GGAAGAAAAA
    TCCTGTCCAG CCGGGTGGCA CCAGCACTCA GGCTACTGTC ACATCTTGAT CACAGAGCAG
    AAAGGCACCT GGAATGCGGC TGCCCAAGCT TGCAGGGAAC AATACCTGGG CAACCTTGTA
    ACTGTATTCT CCAGGCAGCA CATGCGGTGG CTCTGGGACA TTGGTGAGAG AAAGTCCTTT
    TGGATAGGTT TGAACGACCA AGTGCATGCT GGCCACTGGG AGTGGATCGG TGGTGAACCT
    GTTGCCTTCA CCAATGGGAG AAGAGGGCCC TCTCAACGCT CCAAGCTTGG AAAGAGCTGT
    GTTTTGGTTC AAAGACAAGG GAAATGGCAA ACAAAAGATT GTAGGAGAGC CAAACCTCAT
    AATTATGTGT GTTCCAGAAA ACTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OPc109241
    Clone ID Related Accession (Same CDS sequence) XM_024345565.1 , XM_024345568.1 , XM_024345567.1 , XM_024345569.1 , XM_024345566.1
    Accession Version XM_024345567.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6567bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-03-19
    Organism Pan troglodytes(chimpanzee)
    Product FRAS1-related extracellular matrix protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036888.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    ATGAACTCTC TGAGTTGGGG GGCTGCGAAT GCCGTGCTGC TGCTGCTCCT CCTGGCCTGG 
    GCCAGCCCCA CCTTCATCAG CATCAACCGT GGGGTGAGGG TGATGAAGGG CCACTCTGCC
    TTCCTGTCAG GAGATGACCT GAAGTTTGCC ATCCCTAAAG AGAAAGATGC CTGCAAAGTG
    GAAGTTGTGA TGAATGAGCC AATAACCCAG AGGGTTGGGA AACTCACTCC ACAGGTCTTT
    GACTGCCATT TCCTTCCCAA TGAAGTCAAG TATGTTCACA ATGGTTGTCC AATTCTTGAT
    GAAGACACAG TGAAGCTTAG ACTTTACAGA TTTACTGAAA GAGATACCTT CATAGAAACC
    TTTATCCTGT GGGTCTATCT CCTGGAACCA GACTGTAACA TCATCCATAT GAGTAACAAT
    GTGCTGGAGG TGCCTGAATT CAATGGCCTG TCCCAAGCGA TTGATAAAAA TCTGCTCAGA
    TTCGATTATG ATAGGATGGC TAGCCTGGAA TGTACGGTCA GCCTGGACAC TGCGAGAACT
    CGGCTGCCAG CCCATGGCCA GATGGTTCTC GGGGAGCCTC GACCAGAAGA ACCTCGTGGA
    GATCAGCCAC ACAGTTTTTT CCCAGAGTCC CGTATGATGT CTTTTAATGC CTTTCTGGAA
    CTGAGAGCAA AACTGAAGTG TCCAGGTGGA AGCTGTACCC CAGGATTAAA GAAAATAGGA
    AGCCTCAAAG TGAGCTGTGA GGAGTTCCTG CTGATGGGCC TTCGTTATCA GCATCTGGAT
    CCCCCTTCAC CCAACATTGA TTATATCTCC ATCCAACTGG ACCTCACAGA TACCAGGAGT
    AAAATTGTGT ACAAGTCAGA GAGTGCGTGG CTGCCTGTCT ATATCAGAGC TGGAATTCCG
    AATCAGATTC CAAAGGCTGC ATTCATGGCC ATGTTTATTC TGGAAGTGGA TCAGTTCATC
    CTGACCTCCT TGACTACATC AGTTCTGGAC TGTGAAGAAG ATGAGACCCC TAAACCCTTG
    CTGGTGTTCA ACATTACTAA AGCCCCGCTC CAGGGCTATG TGACTCACCT GTTGGATCAC
    ACCAGACCAA TCTCCTCATT CACCTGGAAA GATCTCAGTG ACATGCAGAT CGCCTATCAG
    CCACCAAACA GCAGCCATTC TGAGAGGAGA CATGATGAGG TAGAATTGGA GGTATACGAC
    TTCTTCTTTG AAAGGAGTGC ACCTATGACA GTCCACATCT CCATCAGAAC AGCAGATACA
    AATGCCCCCC GTGTATCCTG GAATACAGGT CTGAATCTCC TTGAGGGGCA GTCTCGAGCC
    ATCACTTGGG AACAGTTTCA GGTTGTCGAC AATGACGACA TCGGTGCTGT CCGGCTAGTC
    ACCGTTGGTG GCCTGCAGCA TGGATGGCTG ACTTTAAGAG GGGGGAAAGG ATTTCTCTTC
    ACCGTGGCTG ACCTCCAGGC TGGAGTTGTT CGCTATCATC ATGATGACAG CGACTCCACC
    AAAGACTTCG TGGTCTTCCG GATATTTGAT GGCCATCACA GCATCCGTCA CAAATTCCCC
    ATCAACGTCT TGCCCAAAGA TGATAGTCCC CCGTTCCTCA TAACCAATGT TGTCATTGAA
    CTGGAGGAGG GGCAGACCAT CCTGATCCAG GGATCCATGC TGCGAGCTTC AGATGTGGAT
    GCCAGTGATG ACTACATCTT CTTCAATATC ACAAAGCCTC CACAGGCTGG GGAGATCATG
    AAGAAGCCAG GGCCAGGACT GATAGGCTAT CCTGTCCCTG GCTTCCTTCA GAGGGATTTG
    TTTAATGGAA TCATTTATTA TCGTCATTTT GGTGGAGAAA TCTTTGAAGA TTCTTTTCAA
    TTTGTCCTGT GGGACAGCCA TGAACCTCCA AATCTTTCAG TGCCACAGGT GGCAACAATC
    CATGTAACTC CAGTGGATGA CCAGCTTCCA AAAGAGGCTC CTGGAGTTTC TCGGCATTTG
    GTTGTCAAGG AAACTGAGGT GGCCTATATA ACTAAGAAAC AGCTACATTT TATAGATTCA
    GAATCATATG ACAGGGAGCT GGTCTACACG ATAACTACTC CTCCATTTTT CTCCTTCAGC
    CACAGACACT TGGATGCTGG GAAATTATTT ATGGTGGACA GCATACCAAA AGTAGTTAAG
    AATCCTACGG CCCTGGAGCT GAGGTCATTC ACTCAGCATG CTGTGAACTA TATGAAAGTG
    GCCTACATGC CCCCCATGCA AGACATTGGT CCCCATTGCA GAGATGTCCA GTTCACATTT
    TCTGTCAGTA ACCAACATGG CGGCACTTTG CATGGGATCT GCTTTAACAT TACAATCCTC
    CCAGTGGACA ATCAAGTTCC TGAGGCGTTC ACCAACCCTC TGAAAGTGAC TGAGGGAGGT
    CAAAGCATCA TCAGCACAGA GCACATTCTG ATTTCTGATG CAGATACCAA GCTGGACAAT
    ATTGACCTCT CCCTGCGGGA ATTGCCTCTG CACGGAAGGG TGGAGCTGAA TGGATTTCCT
    CTAAATTCAG GGGGCACATT TTCTTGGGGC GATCTCCATA CCTTAAAAGT TAGGTATCAA
    CATGATGGAA CTGAAGTTCT TCAGGATGAC CTACTCTTGG AGGTCACCGA TGGCACAAAT
    TCAGCAGAAT TTGTTCTACA TGTTGAGGTA TTCCCTGTCA ACGATGAGCC ACCAGTCTTA
    AAGGCTGACC TCATGCCTGT CATGAATTGC TCAGAGGGAG GAGAGGTGGT CATCACCTCT
    GAATACATTT TTGCTACTGA TGTGGACAGC GATAACTTGA AGTTGATGTT TGTGATTGCT
    CGCGAACCTC AGCATGGGGT GGTGAGGAGA GCTGGAGTCA CAGTGGATCA GTTCTCTCAG
    AGAGATGTTA TCTCAGAGGC CGTGACATAC AAACACACAG GTGGCGAGAT TGGCATGATG
    CCCTGTTTTG ACACCATTAC ATTGGTGGTT TCGGATGGAG AGGCTGGCCC TTTTGTGAAT
    GGCTGTTGCT ACAATGGACC CAATCCATCT GTTCCGCTTC ATGCGTCCTT TCCAGTGTAT
    GATCTCAACA TCACGGTATA CCCAGTAGAC AACCAGCCAC CTTCCATTGC AATAGGTCCC
    ATGTTTGTTG TAGATGAGGG CTGCTCAACA GCCCTTACTG TTAACCATTT GTCCGCCACT
    GACCCTGACA CTGCAGCAGA TGACTTGGAA TTTGTTTTGG TTTCTCCTCC TCAGTTTGGC
    TACCTCGAAA ATATACTCCC TTCTGTGGGT TTTGAAAAAA GCAATATTGG CATAAGTATA
    GATTCATTTC AGTGGAAAGA CATGAACGCT TTTCACATTA ACTATGTGCA GTCCAGGCAT
    CTGAGGATAG AACCAACTGC CGACCAGTTC ACGGTGTACG TCACAGATGG GAAGCAACAC
    TCCTTGGAGA TACCATTTTC TATTATAATC AACCCCACAA ATGATGAAGC TCCTGACTTT
    GTAGTGCAGA ATATTACTGT GTGCGAGGGT CAGATGAAAG AGCTGGACTC TTCCATCATC
    AGCGCTGTGG ACCTGGACAT TCCCCAGGAT GCCCTGCTGT TCAGCATCAC TCAAAAGCCA
    CGCCATGGCC TCCTCATCGA TAGGGGGTTT AGCAAAGACT TCTCTGAGAA TAAGCAGCCA
    GCCAACCCTC ACCAGAAACA TGTACCTGTT CACAGTTTTT CCATGGAACT CCTCAAGACT
    GGAATGAGGT TGACGTACAT GCATGATGAC TCAGAGAGCC TTGCTGATGA TTTTACAATC
    CGTTTGTCAG ATGGGAAACA TAAGATACTT AAAACCATTT CAATAGAGGT CATCCCAGTT
    AATGATGAAA AACCAATGCT GAGCAAGAAG GCTGAAATTG CAATGAATAT GGGTGAAACT
    CGTATTATTT CCAGTGCTAT TCTTTCAGCC ATAGATGAAG ACTCACCCAG GGAGAAGATT
    TACTATGTAT TTGAAAGGCT TCCCCAAAAT GGGCAACTTC AGCTTAAGAT AGGGAGGGAC
    TGGGTTCCTC TCTCCCCTGG CATGAAATGC ACTCAGGAGG AAGTGGATCT GAACTTGCTG
    AGATACACAC ACACCGGGGC AATGGATTCC CAGAATCAAG ATAGCTTCAC CTTCTACCTT
    TGGGATGGCA ACAACAGGTC CCCTGCTCTT GACTGTCAAA TCACCATCAA GGACATGGAA
    AAAGGTGATA TTGTCATCCT TACAAAACCA CTCGTGGTGT CTAAAGGTGA TAGAGGTTTC
    TTAACAACCA CCACTCTCCT GGCTGTGGAC GGAACAGACA AGCCTGAGGA ACTGCTCTAT
    GTCATCACCT CCCCTCCACG ATATGGCCAG ATCGAATATG TTCACTATCC TGGAGTTCCC
    ATTACAAACT TCAGCCAAAT GGATATAGCG GGGCAGACAG TTTGCTATGT ACACAAGAGC
    AAGGTGACTG TCTCCAGTGA CAGATTCAGA TTCATCATCA GCAATGGACT GCGGACCGAG
    CACGGGGTGT TTGAGATCAC ACTGGAGACT GTGGACAGAG CCCTGCCTGT GGTAACCAGG
    AACAAGGGGT TGAGACTGGC CCAAGGGGCC GTGGGCCTGC TTTCCCCTGA CCTCCTTCAG
    CTGACCGACC CTGATACACC TGTGGAGAAC CTCACCTTCC TCTTGGTTCA GCTTCCCCAG
    CATGGCCAGC TCTACCTGTG GGGGACAGGG CTACTTCAAC ACAATTTCAC CCAGCAGGAT
    GTGGACAGCA GGAATGTGGC CTATCGGCAC TCAGGAGGGG ACTCCCAGAC TGACTGCTTT
    ACTTTCATGG CCACAGACGG GACAAACCAA GGCTTTATTG TGAATGGGAG AGTATGGGAA
    GAACCTGTTT TATTCACCAT TCAGGTAGAC CAATTGGACA AAACAGCTCC TCGTATCACA
    CTCTTGCATT CCCCTTCTCA AGTGGGGCTC CTGAAAAATG GCTGCTACGG GATTTACATC
    ACTTCCCGCG TGTTGAAGGC ATCAGACCCT GACACTGAGG ACGATCAGAT CATCTTTAAA
    ATTCTACAAG GCCCAAAACA TGGACATCTG GAGAACACAA CAACAGGTGA ATTTATCCAT
    GAGAAATTTA GCCAAAAGGA CTTAAACAGT AAGACTATTC TTTACATCAT AAACCCATCT
    TTGGAAGTAA ATTCAGATAC CGTGGAATTT CAAATCATGG ACCCCACAGG GAACTCGGCC
    ACTCCTCAAA TTTTGGAACT GAAGTGGTCT CATATTGAAT GGTCACAGAC CGAATATGAA
    GTCTGTGAGA ATGTGGGTGT GTTGCCCTTG GAAATTATCA GAAGGGGATA TTCCATGGAC
    TCGGCCTTTG TGGGTATAAA GGTCAACCAA GTGTCAGCTG CAGATGGAAA AGATTTCACC
    GTGATTCCAT CTAAACTGAT TCAGTTTGAC CCAGGAATGT CAACTAAGAT GTGGAATATA
    GCAATTACCT ATGACGGATT AGAGGAAGAT GATGAGGTCT TTGAAGTAAT TCTGAACTCC
    CCTGTGAATG CAGTTCTTGG CACAAAGACA AAAGCTGCAG TGAAAATTTT GGACTCAAAA
    GGAGGACAAT GCCATCCTTC ATATTCCTCC AACCAAAGCA AGCACAGCAC ATGGGAGAAG
    GGCATTTGGC ATCTGCTGCC CCCAGGGTCT TCCTCATCCA CCACTTCTGG TTCCTTTCAT
    CTGGAAAGAA GACCTCTTCC ATCTTCCATG CAGCTAGCAG TCACCAGGGG AGACACCCTG
    CGGGGCTTTG ATTCTACAGA TCTTTCTCAA AGGAAGCTTA GGACCCGTGG GAATGGCAAA
    ACAGTTCGTC CATCCTCTGT TTATAGAAAT GGAACAGACA TCATCTATAA TTATCATGGG
    ATAGTTTCCT TGAAACTAGA GGATGACAGC TTCCCAACTC ACAAAAGGAA GGCCAAAGTA
    TCCATCATTA GTCAGCCACA AAAGACAATC AAAGTGGCAG AACTGCCTCA AGCAGATAAG
    GTGGAATCCA CAACTGACTC ACACTTCCCC AGACAGGACC AGTTGCCCTC ATTTCCAAAG
    AACTGCACTC TGGAATTAAA GGGACTCTTC CATTTTGAAG AAGGCATCCA GAAGCTGTAT
    CAGTGCAATG GGATTGCCTG GAAAGCCTGG AGTCCCCAAA CCAAGGATGT GGAAGAAAAA
    TCCTGTCCAG CCGGGTGGCA CCAGCACTCA GGCTACTGTC ACATCTTGAT CACAGAGCAG
    AAAGGCACCT GGAATGCGGC TGCCCAAGCT TGCAGGGAAC AATACCTGGG CAACCTTGTA
    ACTGTATTCT CCAGGCAGCA CATGCGGTGG CTCTGGGACA TTGGTGAGAG AAAGTCCTTT
    TGGATAGGTT TGAACGACCA AGTGCATGCT GGCCACTGGG AGTGGATCGG TGGTGAACCT
    GTTGCCTTCA CCAATGGGAG AAGAGGGCCC TCTCAACGCT CCAAGCTTGG AAAGAGCTGT
    GTTTTGGTTC AAAGACAAGG GAAATGGCAA ACAAAAGATT GTAGGAGAGC CAAACCTCAT
    AATTATGTGT GTTCCAGAAA ACTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024201335.1
    CDS1266..7832
    Translation

    Target ORF information:

    RefSeq Version XM_024345567.1
    Organism Pan troglodytes(chimpanzee)
    Definition Pan troglodytes FRAS1 related extracellular matrix 1 (FREM1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024345567.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    ATGAACTCTC TGAGTTGGGG GGCTGCGAAT GCCGTGCTGC TGCTGCTCCT CCTGGCCTGG 
    GCCAGCCCCA CCTTCATCAG CATCAACCGT GGGGTGAGGG TGATGAAGGG CCACTCTGCC
    TTCCTGTCAG GAGATGACCT GAAGTTTGCC ATCCCTAAAG AGAAAGATGC CTGCAAAGTG
    GAAGTTGTGA TGAATGAGCC AATAACCCAG AGGGTTGGGA AACTCACTCC ACAGGTCTTT
    GACTGCCATT TCCTTCCCAA TGAAGTCAAG TATGTTCACA ATGGTTGTCC AATTCTTGAT
    GAAGACACAG TGAAGCTTAG ACTTTACAGA TTTACTGAAA GAGATACCTT CATAGAAACC
    TTTATCCTGT GGGTCTATCT CCTGGAACCA GACTGTAACA TCATCCATAT GAGTAACAAT
    GTGCTGGAGG TGCCTGAATT CAATGGCCTG TCCCAAGCGA TTGATAAAAA TCTGCTCAGA
    TTCGATTATG ATAGGATGGC TAGCCTGGAA TGTACGGTCA GCCTGGACAC TGCGAGAACT
    CGGCTGCCAG CCCATGGCCA GATGGTTCTC GGGGAGCCTC GACCAGAAGA ACCTCGTGGA
    GATCAGCCAC ACAGTTTTTT CCCAGAGTCC CGTATGATGT CTTTTAATGC CTTTCTGGAA
    CTGAGAGCAA AACTGAAGTG TCCAGGTGGA AGCTGTACCC CAGGATTAAA GAAAATAGGA
    AGCCTCAAAG TGAGCTGTGA GGAGTTCCTG CTGATGGGCC TTCGTTATCA GCATCTGGAT
    CCCCCTTCAC CCAACATTGA TTATATCTCC ATCCAACTGG ACCTCACAGA TACCAGGAGT
    AAAATTGTGT ACAAGTCAGA GAGTGCGTGG CTGCCTGTCT ATATCAGAGC TGGAATTCCG
    AATCAGATTC CAAAGGCTGC ATTCATGGCC ATGTTTATTC TGGAAGTGGA TCAGTTCATC
    CTGACCTCCT TGACTACATC AGTTCTGGAC TGTGAAGAAG ATGAGACCCC TAAACCCTTG
    CTGGTGTTCA ACATTACTAA AGCCCCGCTC CAGGGCTATG TGACTCACCT GTTGGATCAC
    ACCAGACCAA TCTCCTCATT CACCTGGAAA GATCTCAGTG ACATGCAGAT CGCCTATCAG
    CCACCAAACA GCAGCCATTC TGAGAGGAGA CATGATGAGG TAGAATTGGA GGTATACGAC
    TTCTTCTTTG AAAGGAGTGC ACCTATGACA GTCCACATCT CCATCAGAAC AGCAGATACA
    AATGCCCCCC GTGTATCCTG GAATACAGGT CTGAATCTCC TTGAGGGGCA GTCTCGAGCC
    ATCACTTGGG AACAGTTTCA GGTTGTCGAC AATGACGACA TCGGTGCTGT CCGGCTAGTC
    ACCGTTGGTG GCCTGCAGCA TGGATGGCTG ACTTTAAGAG GGGGGAAAGG ATTTCTCTTC
    ACCGTGGCTG ACCTCCAGGC TGGAGTTGTT CGCTATCATC ATGATGACAG CGACTCCACC
    AAAGACTTCG TGGTCTTCCG GATATTTGAT GGCCATCACA GCATCCGTCA CAAATTCCCC
    ATCAACGTCT TGCCCAAAGA TGATAGTCCC CCGTTCCTCA TAACCAATGT TGTCATTGAA
    CTGGAGGAGG GGCAGACCAT CCTGATCCAG GGATCCATGC TGCGAGCTTC AGATGTGGAT
    GCCAGTGATG ACTACATCTT CTTCAATATC ACAAAGCCTC CACAGGCTGG GGAGATCATG
    AAGAAGCCAG GGCCAGGACT GATAGGCTAT CCTGTCCCTG GCTTCCTTCA GAGGGATTTG
    TTTAATGGAA TCATTTATTA TCGTCATTTT GGTGGAGAAA TCTTTGAAGA TTCTTTTCAA
    TTTGTCCTGT GGGACAGCCA TGAACCTCCA AATCTTTCAG TGCCACAGGT GGCAACAATC
    CATGTAACTC CAGTGGATGA CCAGCTTCCA AAAGAGGCTC CTGGAGTTTC TCGGCATTTG
    GTTGTCAAGG AAACTGAGGT GGCCTATATA ACTAAGAAAC AGCTACATTT TATAGATTCA
    GAATCATATG ACAGGGAGCT GGTCTACACG ATAACTACTC CTCCATTTTT CTCCTTCAGC
    CACAGACACT TGGATGCTGG GAAATTATTT ATGGTGGACA GCATACCAAA AGTAGTTAAG
    AATCCTACGG CCCTGGAGCT GAGGTCATTC ACTCAGCATG CTGTGAACTA TATGAAAGTG
    GCCTACATGC CCCCCATGCA AGACATTGGT CCCCATTGCA GAGATGTCCA GTTCACATTT
    TCTGTCAGTA ACCAACATGG CGGCACTTTG CATGGGATCT GCTTTAACAT TACAATCCTC
    CCAGTGGACA ATCAAGTTCC TGAGGCGTTC ACCAACCCTC TGAAAGTGAC TGAGGGAGGT
    CAAAGCATCA TCAGCACAGA GCACATTCTG ATTTCTGATG CAGATACCAA GCTGGACAAT
    ATTGACCTCT CCCTGCGGGA ATTGCCTCTG CACGGAAGGG TGGAGCTGAA TGGATTTCCT
    CTAAATTCAG GGGGCACATT TTCTTGGGGC GATCTCCATA CCTTAAAAGT TAGGTATCAA
    CATGATGGAA CTGAAGTTCT TCAGGATGAC CTACTCTTGG AGGTCACCGA TGGCACAAAT
    TCAGCAGAAT TTGTTCTACA TGTTGAGGTA TTCCCTGTCA ACGATGAGCC ACCAGTCTTA
    AAGGCTGACC TCATGCCTGT CATGAATTGC TCAGAGGGAG GAGAGGTGGT CATCACCTCT
    GAATACATTT TTGCTACTGA TGTGGACAGC GATAACTTGA AGTTGATGTT TGTGATTGCT
    CGCGAACCTC AGCATGGGGT GGTGAGGAGA GCTGGAGTCA CAGTGGATCA GTTCTCTCAG
    AGAGATGTTA TCTCAGAGGC CGTGACATAC AAACACACAG GTGGCGAGAT TGGCATGATG
    CCCTGTTTTG ACACCATTAC ATTGGTGGTT TCGGATGGAG AGGCTGGCCC TTTTGTGAAT
    GGCTGTTGCT ACAATGGACC CAATCCATCT GTTCCGCTTC ATGCGTCCTT TCCAGTGTAT
    GATCTCAACA TCACGGTATA CCCAGTAGAC AACCAGCCAC CTTCCATTGC AATAGGTCCC
    ATGTTTGTTG TAGATGAGGG CTGCTCAACA GCCCTTACTG TTAACCATTT GTCCGCCACT
    GACCCTGACA CTGCAGCAGA TGACTTGGAA TTTGTTTTGG TTTCTCCTCC TCAGTTTGGC
    TACCTCGAAA ATATACTCCC TTCTGTGGGT TTTGAAAAAA GCAATATTGG CATAAGTATA
    GATTCATTTC AGTGGAAAGA CATGAACGCT TTTCACATTA ACTATGTGCA GTCCAGGCAT
    CTGAGGATAG AACCAACTGC CGACCAGTTC ACGGTGTACG TCACAGATGG GAAGCAACAC
    TCCTTGGAGA TACCATTTTC TATTATAATC AACCCCACAA ATGATGAAGC TCCTGACTTT
    GTAGTGCAGA ATATTACTGT GTGCGAGGGT CAGATGAAAG AGCTGGACTC TTCCATCATC
    AGCGCTGTGG ACCTGGACAT TCCCCAGGAT GCCCTGCTGT TCAGCATCAC TCAAAAGCCA
    CGCCATGGCC TCCTCATCGA TAGGGGGTTT AGCAAAGACT TCTCTGAGAA TAAGCAGCCA
    GCCAACCCTC ACCAGAAACA TGTACCTGTT CACAGTTTTT CCATGGAACT CCTCAAGACT
    GGAATGAGGT TGACGTACAT GCATGATGAC TCAGAGAGCC TTGCTGATGA TTTTACAATC
    CGTTTGTCAG ATGGGAAACA TAAGATACTT AAAACCATTT CAATAGAGGT CATCCCAGTT
    AATGATGAAA AACCAATGCT GAGCAAGAAG GCTGAAATTG CAATGAATAT GGGTGAAACT
    CGTATTATTT CCAGTGCTAT TCTTTCAGCC ATAGATGAAG ACTCACCCAG GGAGAAGATT
    TACTATGTAT TTGAAAGGCT TCCCCAAAAT GGGCAACTTC AGCTTAAGAT AGGGAGGGAC
    TGGGTTCCTC TCTCCCCTGG CATGAAATGC ACTCAGGAGG AAGTGGATCT GAACTTGCTG
    AGATACACAC ACACCGGGGC AATGGATTCC CAGAATCAAG ATAGCTTCAC CTTCTACCTT
    TGGGATGGCA ACAACAGGTC CCCTGCTCTT GACTGTCAAA TCACCATCAA GGACATGGAA
    AAAGGTGATA TTGTCATCCT TACAAAACCA CTCGTGGTGT CTAAAGGTGA TAGAGGTTTC
    TTAACAACCA CCACTCTCCT GGCTGTGGAC GGAACAGACA AGCCTGAGGA ACTGCTCTAT
    GTCATCACCT CCCCTCCACG ATATGGCCAG ATCGAATATG TTCACTATCC TGGAGTTCCC
    ATTACAAACT TCAGCCAAAT GGATATAGCG GGGCAGACAG TTTGCTATGT ACACAAGAGC
    AAGGTGACTG TCTCCAGTGA CAGATTCAGA TTCATCATCA GCAATGGACT GCGGACCGAG
    CACGGGGTGT TTGAGATCAC ACTGGAGACT GTGGACAGAG CCCTGCCTGT GGTAACCAGG
    AACAAGGGGT TGAGACTGGC CCAAGGGGCC GTGGGCCTGC TTTCCCCTGA CCTCCTTCAG
    CTGACCGACC CTGATACACC TGTGGAGAAC CTCACCTTCC TCTTGGTTCA GCTTCCCCAG
    CATGGCCAGC TCTACCTGTG GGGGACAGGG CTACTTCAAC ACAATTTCAC CCAGCAGGAT
    GTGGACAGCA GGAATGTGGC CTATCGGCAC TCAGGAGGGG ACTCCCAGAC TGACTGCTTT
    ACTTTCATGG CCACAGACGG GACAAACCAA GGCTTTATTG TGAATGGGAG AGTATGGGAA
    GAACCTGTTT TATTCACCAT TCAGGTAGAC CAATTGGACA AAACAGCTCC TCGTATCACA
    CTCTTGCATT CCCCTTCTCA AGTGGGGCTC CTGAAAAATG GCTGCTACGG GATTTACATC
    ACTTCCCGCG TGTTGAAGGC ATCAGACCCT GACACTGAGG ACGATCAGAT CATCTTTAAA
    ATTCTACAAG GCCCAAAACA TGGACATCTG GAGAACACAA CAACAGGTGA ATTTATCCAT
    GAGAAATTTA GCCAAAAGGA CTTAAACAGT AAGACTATTC TTTACATCAT AAACCCATCT
    TTGGAAGTAA ATTCAGATAC CGTGGAATTT CAAATCATGG ACCCCACAGG GAACTCGGCC
    ACTCCTCAAA TTTTGGAACT GAAGTGGTCT CATATTGAAT GGTCACAGAC CGAATATGAA
    GTCTGTGAGA ATGTGGGTGT GTTGCCCTTG GAAATTATCA GAAGGGGATA TTCCATGGAC
    TCGGCCTTTG TGGGTATAAA GGTCAACCAA GTGTCAGCTG CAGATGGAAA AGATTTCACC
    GTGATTCCAT CTAAACTGAT TCAGTTTGAC CCAGGAATGT CAACTAAGAT GTGGAATATA
    GCAATTACCT ATGACGGATT AGAGGAAGAT GATGAGGTCT TTGAAGTAAT TCTGAACTCC
    CCTGTGAATG CAGTTCTTGG CACAAAGACA AAAGCTGCAG TGAAAATTTT GGACTCAAAA
    GGAGGACAAT GCCATCCTTC ATATTCCTCC AACCAAAGCA AGCACAGCAC ATGGGAGAAG
    GGCATTTGGC ATCTGCTGCC CCCAGGGTCT TCCTCATCCA CCACTTCTGG TTCCTTTCAT
    CTGGAAAGAA GACCTCTTCC ATCTTCCATG CAGCTAGCAG TCACCAGGGG AGACACCCTG
    CGGGGCTTTG ATTCTACAGA TCTTTCTCAA AGGAAGCTTA GGACCCGTGG GAATGGCAAA
    ACAGTTCGTC CATCCTCTGT TTATAGAAAT GGAACAGACA TCATCTATAA TTATCATGGG
    ATAGTTTCCT TGAAACTAGA GGATGACAGC TTCCCAACTC ACAAAAGGAA GGCCAAAGTA
    TCCATCATTA GTCAGCCACA AAAGACAATC AAAGTGGCAG AACTGCCTCA AGCAGATAAG
    GTGGAATCCA CAACTGACTC ACACTTCCCC AGACAGGACC AGTTGCCCTC ATTTCCAAAG
    AACTGCACTC TGGAATTAAA GGGACTCTTC CATTTTGAAG AAGGCATCCA GAAGCTGTAT
    CAGTGCAATG GGATTGCCTG GAAAGCCTGG AGTCCCCAAA CCAAGGATGT GGAAGAAAAA
    TCCTGTCCAG CCGGGTGGCA CCAGCACTCA GGCTACTGTC ACATCTTGAT CACAGAGCAG
    AAAGGCACCT GGAATGCGGC TGCCCAAGCT TGCAGGGAAC AATACCTGGG CAACCTTGTA
    ACTGTATTCT CCAGGCAGCA CATGCGGTGG CTCTGGGACA TTGGTGAGAG AAAGTCCTTT
    TGGATAGGTT TGAACGACCA AGTGCATGCT GGCCACTGGG AGTGGATCGG TGGTGAACCT
    GTTGCCTTCA CCAATGGGAG AAGAGGGCCC TCTCAACGCT CCAAGCTTGG AAAGAGCTGT
    GTTTTGGTTC AAAGACAAGG GAAATGGCAA ACAAAAGATT GTAGGAGAGC CAAACCTCAT
    AATTATGTGT GTTCCAGAAA ACTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OPc109241
    Clone ID Related Accession (Same CDS sequence) XM_024345565.1 , XM_024345568.1 , XM_024345567.1 , XM_024345569.1 , XM_024345566.1
    Accession Version XM_024345569.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6567bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-03-19
    Organism Pan troglodytes(chimpanzee)
    Product FRAS1-related extracellular matrix protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036888.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    ATGAACTCTC TGAGTTGGGG GGCTGCGAAT GCCGTGCTGC TGCTGCTCCT CCTGGCCTGG 
    GCCAGCCCCA CCTTCATCAG CATCAACCGT GGGGTGAGGG TGATGAAGGG CCACTCTGCC
    TTCCTGTCAG GAGATGACCT GAAGTTTGCC ATCCCTAAAG AGAAAGATGC CTGCAAAGTG
    GAAGTTGTGA TGAATGAGCC AATAACCCAG AGGGTTGGGA AACTCACTCC ACAGGTCTTT
    GACTGCCATT TCCTTCCCAA TGAAGTCAAG TATGTTCACA ATGGTTGTCC AATTCTTGAT
    GAAGACACAG TGAAGCTTAG ACTTTACAGA TTTACTGAAA GAGATACCTT CATAGAAACC
    TTTATCCTGT GGGTCTATCT CCTGGAACCA GACTGTAACA TCATCCATAT GAGTAACAAT
    GTGCTGGAGG TGCCTGAATT CAATGGCCTG TCCCAAGCGA TTGATAAAAA TCTGCTCAGA
    TTCGATTATG ATAGGATGGC TAGCCTGGAA TGTACGGTCA GCCTGGACAC TGCGAGAACT
    CGGCTGCCAG CCCATGGCCA GATGGTTCTC GGGGAGCCTC GACCAGAAGA ACCTCGTGGA
    GATCAGCCAC ACAGTTTTTT CCCAGAGTCC CGTATGATGT CTTTTAATGC CTTTCTGGAA
    CTGAGAGCAA AACTGAAGTG TCCAGGTGGA AGCTGTACCC CAGGATTAAA GAAAATAGGA
    AGCCTCAAAG TGAGCTGTGA GGAGTTCCTG CTGATGGGCC TTCGTTATCA GCATCTGGAT
    CCCCCTTCAC CCAACATTGA TTATATCTCC ATCCAACTGG ACCTCACAGA TACCAGGAGT
    AAAATTGTGT ACAAGTCAGA GAGTGCGTGG CTGCCTGTCT ATATCAGAGC TGGAATTCCG
    AATCAGATTC CAAAGGCTGC ATTCATGGCC ATGTTTATTC TGGAAGTGGA TCAGTTCATC
    CTGACCTCCT TGACTACATC AGTTCTGGAC TGTGAAGAAG ATGAGACCCC TAAACCCTTG
    CTGGTGTTCA ACATTACTAA AGCCCCGCTC CAGGGCTATG TGACTCACCT GTTGGATCAC
    ACCAGACCAA TCTCCTCATT CACCTGGAAA GATCTCAGTG ACATGCAGAT CGCCTATCAG
    CCACCAAACA GCAGCCATTC TGAGAGGAGA CATGATGAGG TAGAATTGGA GGTATACGAC
    TTCTTCTTTG AAAGGAGTGC ACCTATGACA GTCCACATCT CCATCAGAAC AGCAGATACA
    AATGCCCCCC GTGTATCCTG GAATACAGGT CTGAATCTCC TTGAGGGGCA GTCTCGAGCC
    ATCACTTGGG AACAGTTTCA GGTTGTCGAC AATGACGACA TCGGTGCTGT CCGGCTAGTC
    ACCGTTGGTG GCCTGCAGCA TGGATGGCTG ACTTTAAGAG GGGGGAAAGG ATTTCTCTTC
    ACCGTGGCTG ACCTCCAGGC TGGAGTTGTT CGCTATCATC ATGATGACAG CGACTCCACC
    AAAGACTTCG TGGTCTTCCG GATATTTGAT GGCCATCACA GCATCCGTCA CAAATTCCCC
    ATCAACGTCT TGCCCAAAGA TGATAGTCCC CCGTTCCTCA TAACCAATGT TGTCATTGAA
    CTGGAGGAGG GGCAGACCAT CCTGATCCAG GGATCCATGC TGCGAGCTTC AGATGTGGAT
    GCCAGTGATG ACTACATCTT CTTCAATATC ACAAAGCCTC CACAGGCTGG GGAGATCATG
    AAGAAGCCAG GGCCAGGACT GATAGGCTAT CCTGTCCCTG GCTTCCTTCA GAGGGATTTG
    TTTAATGGAA TCATTTATTA TCGTCATTTT GGTGGAGAAA TCTTTGAAGA TTCTTTTCAA
    TTTGTCCTGT GGGACAGCCA TGAACCTCCA AATCTTTCAG TGCCACAGGT GGCAACAATC
    CATGTAACTC CAGTGGATGA CCAGCTTCCA AAAGAGGCTC CTGGAGTTTC TCGGCATTTG
    GTTGTCAAGG AAACTGAGGT GGCCTATATA ACTAAGAAAC AGCTACATTT TATAGATTCA
    GAATCATATG ACAGGGAGCT GGTCTACACG ATAACTACTC CTCCATTTTT CTCCTTCAGC
    CACAGACACT TGGATGCTGG GAAATTATTT ATGGTGGACA GCATACCAAA AGTAGTTAAG
    AATCCTACGG CCCTGGAGCT GAGGTCATTC ACTCAGCATG CTGTGAACTA TATGAAAGTG
    GCCTACATGC CCCCCATGCA AGACATTGGT CCCCATTGCA GAGATGTCCA GTTCACATTT
    TCTGTCAGTA ACCAACATGG CGGCACTTTG CATGGGATCT GCTTTAACAT TACAATCCTC
    CCAGTGGACA ATCAAGTTCC TGAGGCGTTC ACCAACCCTC TGAAAGTGAC TGAGGGAGGT
    CAAAGCATCA TCAGCACAGA GCACATTCTG ATTTCTGATG CAGATACCAA GCTGGACAAT
    ATTGACCTCT CCCTGCGGGA ATTGCCTCTG CACGGAAGGG TGGAGCTGAA TGGATTTCCT
    CTAAATTCAG GGGGCACATT TTCTTGGGGC GATCTCCATA CCTTAAAAGT TAGGTATCAA
    CATGATGGAA CTGAAGTTCT TCAGGATGAC CTACTCTTGG AGGTCACCGA TGGCACAAAT
    TCAGCAGAAT TTGTTCTACA TGTTGAGGTA TTCCCTGTCA ACGATGAGCC ACCAGTCTTA
    AAGGCTGACC TCATGCCTGT CATGAATTGC TCAGAGGGAG GAGAGGTGGT CATCACCTCT
    GAATACATTT TTGCTACTGA TGTGGACAGC GATAACTTGA AGTTGATGTT TGTGATTGCT
    CGCGAACCTC AGCATGGGGT GGTGAGGAGA GCTGGAGTCA CAGTGGATCA GTTCTCTCAG
    AGAGATGTTA TCTCAGAGGC CGTGACATAC AAACACACAG GTGGCGAGAT TGGCATGATG
    CCCTGTTTTG ACACCATTAC ATTGGTGGTT TCGGATGGAG AGGCTGGCCC TTTTGTGAAT
    GGCTGTTGCT ACAATGGACC CAATCCATCT GTTCCGCTTC ATGCGTCCTT TCCAGTGTAT
    GATCTCAACA TCACGGTATA CCCAGTAGAC AACCAGCCAC CTTCCATTGC AATAGGTCCC
    ATGTTTGTTG TAGATGAGGG CTGCTCAACA GCCCTTACTG TTAACCATTT GTCCGCCACT
    GACCCTGACA CTGCAGCAGA TGACTTGGAA TTTGTTTTGG TTTCTCCTCC TCAGTTTGGC
    TACCTCGAAA ATATACTCCC TTCTGTGGGT TTTGAAAAAA GCAATATTGG CATAAGTATA
    GATTCATTTC AGTGGAAAGA CATGAACGCT TTTCACATTA ACTATGTGCA GTCCAGGCAT
    CTGAGGATAG AACCAACTGC CGACCAGTTC ACGGTGTACG TCACAGATGG GAAGCAACAC
    TCCTTGGAGA TACCATTTTC TATTATAATC AACCCCACAA ATGATGAAGC TCCTGACTTT
    GTAGTGCAGA ATATTACTGT GTGCGAGGGT CAGATGAAAG AGCTGGACTC TTCCATCATC
    AGCGCTGTGG ACCTGGACAT TCCCCAGGAT GCCCTGCTGT TCAGCATCAC TCAAAAGCCA
    CGCCATGGCC TCCTCATCGA TAGGGGGTTT AGCAAAGACT TCTCTGAGAA TAAGCAGCCA
    GCCAACCCTC ACCAGAAACA TGTACCTGTT CACAGTTTTT CCATGGAACT CCTCAAGACT
    GGAATGAGGT TGACGTACAT GCATGATGAC TCAGAGAGCC TTGCTGATGA TTTTACAATC
    CGTTTGTCAG ATGGGAAACA TAAGATACTT AAAACCATTT CAATAGAGGT CATCCCAGTT
    AATGATGAAA AACCAATGCT GAGCAAGAAG GCTGAAATTG CAATGAATAT GGGTGAAACT
    CGTATTATTT CCAGTGCTAT TCTTTCAGCC ATAGATGAAG ACTCACCCAG GGAGAAGATT
    TACTATGTAT TTGAAAGGCT TCCCCAAAAT GGGCAACTTC AGCTTAAGAT AGGGAGGGAC
    TGGGTTCCTC TCTCCCCTGG CATGAAATGC ACTCAGGAGG AAGTGGATCT GAACTTGCTG
    AGATACACAC ACACCGGGGC AATGGATTCC CAGAATCAAG ATAGCTTCAC CTTCTACCTT
    TGGGATGGCA ACAACAGGTC CCCTGCTCTT GACTGTCAAA TCACCATCAA GGACATGGAA
    AAAGGTGATA TTGTCATCCT TACAAAACCA CTCGTGGTGT CTAAAGGTGA TAGAGGTTTC
    TTAACAACCA CCACTCTCCT GGCTGTGGAC GGAACAGACA AGCCTGAGGA ACTGCTCTAT
    GTCATCACCT CCCCTCCACG ATATGGCCAG ATCGAATATG TTCACTATCC TGGAGTTCCC
    ATTACAAACT TCAGCCAAAT GGATATAGCG GGGCAGACAG TTTGCTATGT ACACAAGAGC
    AAGGTGACTG TCTCCAGTGA CAGATTCAGA TTCATCATCA GCAATGGACT GCGGACCGAG
    CACGGGGTGT TTGAGATCAC ACTGGAGACT GTGGACAGAG CCCTGCCTGT GGTAACCAGG
    AACAAGGGGT TGAGACTGGC CCAAGGGGCC GTGGGCCTGC TTTCCCCTGA CCTCCTTCAG
    CTGACCGACC CTGATACACC TGTGGAGAAC CTCACCTTCC TCTTGGTTCA GCTTCCCCAG
    CATGGCCAGC TCTACCTGTG GGGGACAGGG CTACTTCAAC ACAATTTCAC CCAGCAGGAT
    GTGGACAGCA GGAATGTGGC CTATCGGCAC TCAGGAGGGG ACTCCCAGAC TGACTGCTTT
    ACTTTCATGG CCACAGACGG GACAAACCAA GGCTTTATTG TGAATGGGAG AGTATGGGAA
    GAACCTGTTT TATTCACCAT TCAGGTAGAC CAATTGGACA AAACAGCTCC TCGTATCACA
    CTCTTGCATT CCCCTTCTCA AGTGGGGCTC CTGAAAAATG GCTGCTACGG GATTTACATC
    ACTTCCCGCG TGTTGAAGGC ATCAGACCCT GACACTGAGG ACGATCAGAT CATCTTTAAA
    ATTCTACAAG GCCCAAAACA TGGACATCTG GAGAACACAA CAACAGGTGA ATTTATCCAT
    GAGAAATTTA GCCAAAAGGA CTTAAACAGT AAGACTATTC TTTACATCAT AAACCCATCT
    TTGGAAGTAA ATTCAGATAC CGTGGAATTT CAAATCATGG ACCCCACAGG GAACTCGGCC
    ACTCCTCAAA TTTTGGAACT GAAGTGGTCT CATATTGAAT GGTCACAGAC CGAATATGAA
    GTCTGTGAGA ATGTGGGTGT GTTGCCCTTG GAAATTATCA GAAGGGGATA TTCCATGGAC
    TCGGCCTTTG TGGGTATAAA GGTCAACCAA GTGTCAGCTG CAGATGGAAA AGATTTCACC
    GTGATTCCAT CTAAACTGAT TCAGTTTGAC CCAGGAATGT CAACTAAGAT GTGGAATATA
    GCAATTACCT ATGACGGATT AGAGGAAGAT GATGAGGTCT TTGAAGTAAT TCTGAACTCC
    CCTGTGAATG CAGTTCTTGG CACAAAGACA AAAGCTGCAG TGAAAATTTT GGACTCAAAA
    GGAGGACAAT GCCATCCTTC ATATTCCTCC AACCAAAGCA AGCACAGCAC ATGGGAGAAG
    GGCATTTGGC ATCTGCTGCC CCCAGGGTCT TCCTCATCCA CCACTTCTGG TTCCTTTCAT
    CTGGAAAGAA GACCTCTTCC ATCTTCCATG CAGCTAGCAG TCACCAGGGG AGACACCCTG
    CGGGGCTTTG ATTCTACAGA TCTTTCTCAA AGGAAGCTTA GGACCCGTGG GAATGGCAAA
    ACAGTTCGTC CATCCTCTGT TTATAGAAAT GGAACAGACA TCATCTATAA TTATCATGGG
    ATAGTTTCCT TGAAACTAGA GGATGACAGC TTCCCAACTC ACAAAAGGAA GGCCAAAGTA
    TCCATCATTA GTCAGCCACA AAAGACAATC AAAGTGGCAG AACTGCCTCA AGCAGATAAG
    GTGGAATCCA CAACTGACTC ACACTTCCCC AGACAGGACC AGTTGCCCTC ATTTCCAAAG
    AACTGCACTC TGGAATTAAA GGGACTCTTC CATTTTGAAG AAGGCATCCA GAAGCTGTAT
    CAGTGCAATG GGATTGCCTG GAAAGCCTGG AGTCCCCAAA CCAAGGATGT GGAAGAAAAA
    TCCTGTCCAG CCGGGTGGCA CCAGCACTCA GGCTACTGTC ACATCTTGAT CACAGAGCAG
    AAAGGCACCT GGAATGCGGC TGCCCAAGCT TGCAGGGAAC AATACCTGGG CAACCTTGTA
    ACTGTATTCT CCAGGCAGCA CATGCGGTGG CTCTGGGACA TTGGTGAGAG AAAGTCCTTT
    TGGATAGGTT TGAACGACCA AGTGCATGCT GGCCACTGGG AGTGGATCGG TGGTGAACCT
    GTTGCCTTCA CCAATGGGAG AAGAGGGCCC TCTCAACGCT CCAAGCTTGG AAAGAGCTGT
    GTTTTGGTTC AAAGACAAGG GAAATGGCAA ACAAAAGATT GTAGGAGAGC CAAACCTCAT
    AATTATGTGT GTTCCAGAAA ACTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024201337.1
    CDS854..7420
    Translation

    Target ORF information:

    RefSeq Version XM_024345569.1
    Organism Pan troglodytes(chimpanzee)
    Definition Pan troglodytes FRAS1 related extracellular matrix 1 (FREM1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024345569.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    ATGAACTCTC TGAGTTGGGG GGCTGCGAAT GCCGTGCTGC TGCTGCTCCT CCTGGCCTGG 
    GCCAGCCCCA CCTTCATCAG CATCAACCGT GGGGTGAGGG TGATGAAGGG CCACTCTGCC
    TTCCTGTCAG GAGATGACCT GAAGTTTGCC ATCCCTAAAG AGAAAGATGC CTGCAAAGTG
    GAAGTTGTGA TGAATGAGCC AATAACCCAG AGGGTTGGGA AACTCACTCC ACAGGTCTTT
    GACTGCCATT TCCTTCCCAA TGAAGTCAAG TATGTTCACA ATGGTTGTCC AATTCTTGAT
    GAAGACACAG TGAAGCTTAG ACTTTACAGA TTTACTGAAA GAGATACCTT CATAGAAACC
    TTTATCCTGT GGGTCTATCT CCTGGAACCA GACTGTAACA TCATCCATAT GAGTAACAAT
    GTGCTGGAGG TGCCTGAATT CAATGGCCTG TCCCAAGCGA TTGATAAAAA TCTGCTCAGA
    TTCGATTATG ATAGGATGGC TAGCCTGGAA TGTACGGTCA GCCTGGACAC TGCGAGAACT
    CGGCTGCCAG CCCATGGCCA GATGGTTCTC GGGGAGCCTC GACCAGAAGA ACCTCGTGGA
    GATCAGCCAC ACAGTTTTTT CCCAGAGTCC CGTATGATGT CTTTTAATGC CTTTCTGGAA
    CTGAGAGCAA AACTGAAGTG TCCAGGTGGA AGCTGTACCC CAGGATTAAA GAAAATAGGA
    AGCCTCAAAG TGAGCTGTGA GGAGTTCCTG CTGATGGGCC TTCGTTATCA GCATCTGGAT
    CCCCCTTCAC CCAACATTGA TTATATCTCC ATCCAACTGG ACCTCACAGA TACCAGGAGT
    AAAATTGTGT ACAAGTCAGA GAGTGCGTGG CTGCCTGTCT ATATCAGAGC TGGAATTCCG
    AATCAGATTC CAAAGGCTGC ATTCATGGCC ATGTTTATTC TGGAAGTGGA TCAGTTCATC
    CTGACCTCCT TGACTACATC AGTTCTGGAC TGTGAAGAAG ATGAGACCCC TAAACCCTTG
    CTGGTGTTCA ACATTACTAA AGCCCCGCTC CAGGGCTATG TGACTCACCT GTTGGATCAC
    ACCAGACCAA TCTCCTCATT CACCTGGAAA GATCTCAGTG ACATGCAGAT CGCCTATCAG
    CCACCAAACA GCAGCCATTC TGAGAGGAGA CATGATGAGG TAGAATTGGA GGTATACGAC
    TTCTTCTTTG AAAGGAGTGC ACCTATGACA GTCCACATCT CCATCAGAAC AGCAGATACA
    AATGCCCCCC GTGTATCCTG GAATACAGGT CTGAATCTCC TTGAGGGGCA GTCTCGAGCC
    ATCACTTGGG AACAGTTTCA GGTTGTCGAC AATGACGACA TCGGTGCTGT CCGGCTAGTC
    ACCGTTGGTG GCCTGCAGCA TGGATGGCTG ACTTTAAGAG GGGGGAAAGG ATTTCTCTTC
    ACCGTGGCTG ACCTCCAGGC TGGAGTTGTT CGCTATCATC ATGATGACAG CGACTCCACC
    AAAGACTTCG TGGTCTTCCG GATATTTGAT GGCCATCACA GCATCCGTCA CAAATTCCCC
    ATCAACGTCT TGCCCAAAGA TGATAGTCCC CCGTTCCTCA TAACCAATGT TGTCATTGAA
    CTGGAGGAGG GGCAGACCAT CCTGATCCAG GGATCCATGC TGCGAGCTTC AGATGTGGAT
    GCCAGTGATG ACTACATCTT CTTCAATATC ACAAAGCCTC CACAGGCTGG GGAGATCATG
    AAGAAGCCAG GGCCAGGACT GATAGGCTAT CCTGTCCCTG GCTTCCTTCA GAGGGATTTG
    TTTAATGGAA TCATTTATTA TCGTCATTTT GGTGGAGAAA TCTTTGAAGA TTCTTTTCAA
    TTTGTCCTGT GGGACAGCCA TGAACCTCCA AATCTTTCAG TGCCACAGGT GGCAACAATC
    CATGTAACTC CAGTGGATGA CCAGCTTCCA AAAGAGGCTC CTGGAGTTTC TCGGCATTTG
    GTTGTCAAGG AAACTGAGGT GGCCTATATA ACTAAGAAAC AGCTACATTT TATAGATTCA
    GAATCATATG ACAGGGAGCT GGTCTACACG ATAACTACTC CTCCATTTTT CTCCTTCAGC
    CACAGACACT TGGATGCTGG GAAATTATTT ATGGTGGACA GCATACCAAA AGTAGTTAAG
    AATCCTACGG CCCTGGAGCT GAGGTCATTC ACTCAGCATG CTGTGAACTA TATGAAAGTG
    GCCTACATGC CCCCCATGCA AGACATTGGT CCCCATTGCA GAGATGTCCA GTTCACATTT
    TCTGTCAGTA ACCAACATGG CGGCACTTTG CATGGGATCT GCTTTAACAT TACAATCCTC
    CCAGTGGACA ATCAAGTTCC TGAGGCGTTC ACCAACCCTC TGAAAGTGAC TGAGGGAGGT
    CAAAGCATCA TCAGCACAGA GCACATTCTG ATTTCTGATG CAGATACCAA GCTGGACAAT
    ATTGACCTCT CCCTGCGGGA ATTGCCTCTG CACGGAAGGG TGGAGCTGAA TGGATTTCCT
    CTAAATTCAG GGGGCACATT TTCTTGGGGC GATCTCCATA CCTTAAAAGT TAGGTATCAA
    CATGATGGAA CTGAAGTTCT TCAGGATGAC CTACTCTTGG AGGTCACCGA TGGCACAAAT
    TCAGCAGAAT TTGTTCTACA TGTTGAGGTA TTCCCTGTCA ACGATGAGCC ACCAGTCTTA
    AAGGCTGACC TCATGCCTGT CATGAATTGC TCAGAGGGAG GAGAGGTGGT CATCACCTCT
    GAATACATTT TTGCTACTGA TGTGGACAGC GATAACTTGA AGTTGATGTT TGTGATTGCT
    CGCGAACCTC AGCATGGGGT GGTGAGGAGA GCTGGAGTCA CAGTGGATCA GTTCTCTCAG
    AGAGATGTTA TCTCAGAGGC CGTGACATAC AAACACACAG GTGGCGAGAT TGGCATGATG
    CCCTGTTTTG ACACCATTAC ATTGGTGGTT TCGGATGGAG AGGCTGGCCC TTTTGTGAAT
    GGCTGTTGCT ACAATGGACC CAATCCATCT GTTCCGCTTC ATGCGTCCTT TCCAGTGTAT
    GATCTCAACA TCACGGTATA CCCAGTAGAC AACCAGCCAC CTTCCATTGC AATAGGTCCC
    ATGTTTGTTG TAGATGAGGG CTGCTCAACA GCCCTTACTG TTAACCATTT GTCCGCCACT
    GACCCTGACA CTGCAGCAGA TGACTTGGAA TTTGTTTTGG TTTCTCCTCC TCAGTTTGGC
    TACCTCGAAA ATATACTCCC TTCTGTGGGT TTTGAAAAAA GCAATATTGG CATAAGTATA
    GATTCATTTC AGTGGAAAGA CATGAACGCT TTTCACATTA ACTATGTGCA GTCCAGGCAT
    CTGAGGATAG AACCAACTGC CGACCAGTTC ACGGTGTACG TCACAGATGG GAAGCAACAC
    TCCTTGGAGA TACCATTTTC TATTATAATC AACCCCACAA ATGATGAAGC TCCTGACTTT
    GTAGTGCAGA ATATTACTGT GTGCGAGGGT CAGATGAAAG AGCTGGACTC TTCCATCATC
    AGCGCTGTGG ACCTGGACAT TCCCCAGGAT GCCCTGCTGT TCAGCATCAC TCAAAAGCCA
    CGCCATGGCC TCCTCATCGA TAGGGGGTTT AGCAAAGACT TCTCTGAGAA TAAGCAGCCA
    GCCAACCCTC ACCAGAAACA TGTACCTGTT CACAGTTTTT CCATGGAACT CCTCAAGACT
    GGAATGAGGT TGACGTACAT GCATGATGAC TCAGAGAGCC TTGCTGATGA TTTTACAATC
    CGTTTGTCAG ATGGGAAACA TAAGATACTT AAAACCATTT CAATAGAGGT CATCCCAGTT
    AATGATGAAA AACCAATGCT GAGCAAGAAG GCTGAAATTG CAATGAATAT GGGTGAAACT
    CGTATTATTT CCAGTGCTAT TCTTTCAGCC ATAGATGAAG ACTCACCCAG GGAGAAGATT
    TACTATGTAT TTGAAAGGCT TCCCCAAAAT GGGCAACTTC AGCTTAAGAT AGGGAGGGAC
    TGGGTTCCTC TCTCCCCTGG CATGAAATGC ACTCAGGAGG AAGTGGATCT GAACTTGCTG
    AGATACACAC ACACCGGGGC AATGGATTCC CAGAATCAAG ATAGCTTCAC CTTCTACCTT
    TGGGATGGCA ACAACAGGTC CCCTGCTCTT GACTGTCAAA TCACCATCAA GGACATGGAA
    AAAGGTGATA TTGTCATCCT TACAAAACCA CTCGTGGTGT CTAAAGGTGA TAGAGGTTTC
    TTAACAACCA CCACTCTCCT GGCTGTGGAC GGAACAGACA AGCCTGAGGA ACTGCTCTAT
    GTCATCACCT CCCCTCCACG ATATGGCCAG ATCGAATATG TTCACTATCC TGGAGTTCCC
    ATTACAAACT TCAGCCAAAT GGATATAGCG GGGCAGACAG TTTGCTATGT ACACAAGAGC
    AAGGTGACTG TCTCCAGTGA CAGATTCAGA TTCATCATCA GCAATGGACT GCGGACCGAG
    CACGGGGTGT TTGAGATCAC ACTGGAGACT GTGGACAGAG CCCTGCCTGT GGTAACCAGG
    AACAAGGGGT TGAGACTGGC CCAAGGGGCC GTGGGCCTGC TTTCCCCTGA CCTCCTTCAG
    CTGACCGACC CTGATACACC TGTGGAGAAC CTCACCTTCC TCTTGGTTCA GCTTCCCCAG
    CATGGCCAGC TCTACCTGTG GGGGACAGGG CTACTTCAAC ACAATTTCAC CCAGCAGGAT
    GTGGACAGCA GGAATGTGGC CTATCGGCAC TCAGGAGGGG ACTCCCAGAC TGACTGCTTT
    ACTTTCATGG CCACAGACGG GACAAACCAA GGCTTTATTG TGAATGGGAG AGTATGGGAA
    GAACCTGTTT TATTCACCAT TCAGGTAGAC CAATTGGACA AAACAGCTCC TCGTATCACA
    CTCTTGCATT CCCCTTCTCA AGTGGGGCTC CTGAAAAATG GCTGCTACGG GATTTACATC
    ACTTCCCGCG TGTTGAAGGC ATCAGACCCT GACACTGAGG ACGATCAGAT CATCTTTAAA
    ATTCTACAAG GCCCAAAACA TGGACATCTG GAGAACACAA CAACAGGTGA ATTTATCCAT
    GAGAAATTTA GCCAAAAGGA CTTAAACAGT AAGACTATTC TTTACATCAT AAACCCATCT
    TTGGAAGTAA ATTCAGATAC CGTGGAATTT CAAATCATGG ACCCCACAGG GAACTCGGCC
    ACTCCTCAAA TTTTGGAACT GAAGTGGTCT CATATTGAAT GGTCACAGAC CGAATATGAA
    GTCTGTGAGA ATGTGGGTGT GTTGCCCTTG GAAATTATCA GAAGGGGATA TTCCATGGAC
    TCGGCCTTTG TGGGTATAAA GGTCAACCAA GTGTCAGCTG CAGATGGAAA AGATTTCACC
    GTGATTCCAT CTAAACTGAT TCAGTTTGAC CCAGGAATGT CAACTAAGAT GTGGAATATA
    GCAATTACCT ATGACGGATT AGAGGAAGAT GATGAGGTCT TTGAAGTAAT TCTGAACTCC
    CCTGTGAATG CAGTTCTTGG CACAAAGACA AAAGCTGCAG TGAAAATTTT GGACTCAAAA
    GGAGGACAAT GCCATCCTTC ATATTCCTCC AACCAAAGCA AGCACAGCAC ATGGGAGAAG
    GGCATTTGGC ATCTGCTGCC CCCAGGGTCT TCCTCATCCA CCACTTCTGG TTCCTTTCAT
    CTGGAAAGAA GACCTCTTCC ATCTTCCATG CAGCTAGCAG TCACCAGGGG AGACACCCTG
    CGGGGCTTTG ATTCTACAGA TCTTTCTCAA AGGAAGCTTA GGACCCGTGG GAATGGCAAA
    ACAGTTCGTC CATCCTCTGT TTATAGAAAT GGAACAGACA TCATCTATAA TTATCATGGG
    ATAGTTTCCT TGAAACTAGA GGATGACAGC TTCCCAACTC ACAAAAGGAA GGCCAAAGTA
    TCCATCATTA GTCAGCCACA AAAGACAATC AAAGTGGCAG AACTGCCTCA AGCAGATAAG
    GTGGAATCCA CAACTGACTC ACACTTCCCC AGACAGGACC AGTTGCCCTC ATTTCCAAAG
    AACTGCACTC TGGAATTAAA GGGACTCTTC CATTTTGAAG AAGGCATCCA GAAGCTGTAT
    CAGTGCAATG GGATTGCCTG GAAAGCCTGG AGTCCCCAAA CCAAGGATGT GGAAGAAAAA
    TCCTGTCCAG CCGGGTGGCA CCAGCACTCA GGCTACTGTC ACATCTTGAT CACAGAGCAG
    AAAGGCACCT GGAATGCGGC TGCCCAAGCT TGCAGGGAAC AATACCTGGG CAACCTTGTA
    ACTGTATTCT CCAGGCAGCA CATGCGGTGG CTCTGGGACA TTGGTGAGAG AAAGTCCTTT
    TGGATAGGTT TGAACGACCA AGTGCATGCT GGCCACTGGG AGTGGATCGG TGGTGAACCT
    GTTGCCTTCA CCAATGGGAG AAGAGGGCCC TCTCAACGCT CCAAGCTTGG AAAGAGCTGT
    GTTTTGGTTC AAAGACAAGG GAAATGGCAA ACAAAAGATT GTAGGAGAGC CAAACCTCAT
    AATTATGTGT GTTCCAGAAA ACTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OPc109241
    Clone ID Related Accession (Same CDS sequence) XM_024345565.1 , XM_024345568.1 , XM_024345567.1 , XM_024345569.1 , XM_024345566.1
    Accession Version XM_024345566.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6567bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-03-19
    Organism Pan troglodytes(chimpanzee)
    Product FRAS1-related extracellular matrix protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036888.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    ATGAACTCTC TGAGTTGGGG GGCTGCGAAT GCCGTGCTGC TGCTGCTCCT CCTGGCCTGG 
    GCCAGCCCCA CCTTCATCAG CATCAACCGT GGGGTGAGGG TGATGAAGGG CCACTCTGCC
    TTCCTGTCAG GAGATGACCT GAAGTTTGCC ATCCCTAAAG AGAAAGATGC CTGCAAAGTG
    GAAGTTGTGA TGAATGAGCC AATAACCCAG AGGGTTGGGA AACTCACTCC ACAGGTCTTT
    GACTGCCATT TCCTTCCCAA TGAAGTCAAG TATGTTCACA ATGGTTGTCC AATTCTTGAT
    GAAGACACAG TGAAGCTTAG ACTTTACAGA TTTACTGAAA GAGATACCTT CATAGAAACC
    TTTATCCTGT GGGTCTATCT CCTGGAACCA GACTGTAACA TCATCCATAT GAGTAACAAT
    GTGCTGGAGG TGCCTGAATT CAATGGCCTG TCCCAAGCGA TTGATAAAAA TCTGCTCAGA
    TTCGATTATG ATAGGATGGC TAGCCTGGAA TGTACGGTCA GCCTGGACAC TGCGAGAACT
    CGGCTGCCAG CCCATGGCCA GATGGTTCTC GGGGAGCCTC GACCAGAAGA ACCTCGTGGA
    GATCAGCCAC ACAGTTTTTT CCCAGAGTCC CGTATGATGT CTTTTAATGC CTTTCTGGAA
    CTGAGAGCAA AACTGAAGTG TCCAGGTGGA AGCTGTACCC CAGGATTAAA GAAAATAGGA
    AGCCTCAAAG TGAGCTGTGA GGAGTTCCTG CTGATGGGCC TTCGTTATCA GCATCTGGAT
    CCCCCTTCAC CCAACATTGA TTATATCTCC ATCCAACTGG ACCTCACAGA TACCAGGAGT
    AAAATTGTGT ACAAGTCAGA GAGTGCGTGG CTGCCTGTCT ATATCAGAGC TGGAATTCCG
    AATCAGATTC CAAAGGCTGC ATTCATGGCC ATGTTTATTC TGGAAGTGGA TCAGTTCATC
    CTGACCTCCT TGACTACATC AGTTCTGGAC TGTGAAGAAG ATGAGACCCC TAAACCCTTG
    CTGGTGTTCA ACATTACTAA AGCCCCGCTC CAGGGCTATG TGACTCACCT GTTGGATCAC
    ACCAGACCAA TCTCCTCATT CACCTGGAAA GATCTCAGTG ACATGCAGAT CGCCTATCAG
    CCACCAAACA GCAGCCATTC TGAGAGGAGA CATGATGAGG TAGAATTGGA GGTATACGAC
    TTCTTCTTTG AAAGGAGTGC ACCTATGACA GTCCACATCT CCATCAGAAC AGCAGATACA
    AATGCCCCCC GTGTATCCTG GAATACAGGT CTGAATCTCC TTGAGGGGCA GTCTCGAGCC
    ATCACTTGGG AACAGTTTCA GGTTGTCGAC AATGACGACA TCGGTGCTGT CCGGCTAGTC
    ACCGTTGGTG GCCTGCAGCA TGGATGGCTG ACTTTAAGAG GGGGGAAAGG ATTTCTCTTC
    ACCGTGGCTG ACCTCCAGGC TGGAGTTGTT CGCTATCATC ATGATGACAG CGACTCCACC
    AAAGACTTCG TGGTCTTCCG GATATTTGAT GGCCATCACA GCATCCGTCA CAAATTCCCC
    ATCAACGTCT TGCCCAAAGA TGATAGTCCC CCGTTCCTCA TAACCAATGT TGTCATTGAA
    CTGGAGGAGG GGCAGACCAT CCTGATCCAG GGATCCATGC TGCGAGCTTC AGATGTGGAT
    GCCAGTGATG ACTACATCTT CTTCAATATC ACAAAGCCTC CACAGGCTGG GGAGATCATG
    AAGAAGCCAG GGCCAGGACT GATAGGCTAT CCTGTCCCTG GCTTCCTTCA GAGGGATTTG
    TTTAATGGAA TCATTTATTA TCGTCATTTT GGTGGAGAAA TCTTTGAAGA TTCTTTTCAA
    TTTGTCCTGT GGGACAGCCA TGAACCTCCA AATCTTTCAG TGCCACAGGT GGCAACAATC
    CATGTAACTC CAGTGGATGA CCAGCTTCCA AAAGAGGCTC CTGGAGTTTC TCGGCATTTG
    GTTGTCAAGG AAACTGAGGT GGCCTATATA ACTAAGAAAC AGCTACATTT TATAGATTCA
    GAATCATATG ACAGGGAGCT GGTCTACACG ATAACTACTC CTCCATTTTT CTCCTTCAGC
    CACAGACACT TGGATGCTGG GAAATTATTT ATGGTGGACA GCATACCAAA AGTAGTTAAG
    AATCCTACGG CCCTGGAGCT GAGGTCATTC ACTCAGCATG CTGTGAACTA TATGAAAGTG
    GCCTACATGC CCCCCATGCA AGACATTGGT CCCCATTGCA GAGATGTCCA GTTCACATTT
    TCTGTCAGTA ACCAACATGG CGGCACTTTG CATGGGATCT GCTTTAACAT TACAATCCTC
    CCAGTGGACA ATCAAGTTCC TGAGGCGTTC ACCAACCCTC TGAAAGTGAC TGAGGGAGGT
    CAAAGCATCA TCAGCACAGA GCACATTCTG ATTTCTGATG CAGATACCAA GCTGGACAAT
    ATTGACCTCT CCCTGCGGGA ATTGCCTCTG CACGGAAGGG TGGAGCTGAA TGGATTTCCT
    CTAAATTCAG GGGGCACATT TTCTTGGGGC GATCTCCATA CCTTAAAAGT TAGGTATCAA
    CATGATGGAA CTGAAGTTCT TCAGGATGAC CTACTCTTGG AGGTCACCGA TGGCACAAAT
    TCAGCAGAAT TTGTTCTACA TGTTGAGGTA TTCCCTGTCA ACGATGAGCC ACCAGTCTTA
    AAGGCTGACC TCATGCCTGT CATGAATTGC TCAGAGGGAG GAGAGGTGGT CATCACCTCT
    GAATACATTT TTGCTACTGA TGTGGACAGC GATAACTTGA AGTTGATGTT TGTGATTGCT
    CGCGAACCTC AGCATGGGGT GGTGAGGAGA GCTGGAGTCA CAGTGGATCA GTTCTCTCAG
    AGAGATGTTA TCTCAGAGGC CGTGACATAC AAACACACAG GTGGCGAGAT TGGCATGATG
    CCCTGTTTTG ACACCATTAC ATTGGTGGTT TCGGATGGAG AGGCTGGCCC TTTTGTGAAT
    GGCTGTTGCT ACAATGGACC CAATCCATCT GTTCCGCTTC ATGCGTCCTT TCCAGTGTAT
    GATCTCAACA TCACGGTATA CCCAGTAGAC AACCAGCCAC CTTCCATTGC AATAGGTCCC
    ATGTTTGTTG TAGATGAGGG CTGCTCAACA GCCCTTACTG TTAACCATTT GTCCGCCACT
    GACCCTGACA CTGCAGCAGA TGACTTGGAA TTTGTTTTGG TTTCTCCTCC TCAGTTTGGC
    TACCTCGAAA ATATACTCCC TTCTGTGGGT TTTGAAAAAA GCAATATTGG CATAAGTATA
    GATTCATTTC AGTGGAAAGA CATGAACGCT TTTCACATTA ACTATGTGCA GTCCAGGCAT
    CTGAGGATAG AACCAACTGC CGACCAGTTC ACGGTGTACG TCACAGATGG GAAGCAACAC
    TCCTTGGAGA TACCATTTTC TATTATAATC AACCCCACAA ATGATGAAGC TCCTGACTTT
    GTAGTGCAGA ATATTACTGT GTGCGAGGGT CAGATGAAAG AGCTGGACTC TTCCATCATC
    AGCGCTGTGG ACCTGGACAT TCCCCAGGAT GCCCTGCTGT TCAGCATCAC TCAAAAGCCA
    CGCCATGGCC TCCTCATCGA TAGGGGGTTT AGCAAAGACT TCTCTGAGAA TAAGCAGCCA
    GCCAACCCTC ACCAGAAACA TGTACCTGTT CACAGTTTTT CCATGGAACT CCTCAAGACT
    GGAATGAGGT TGACGTACAT GCATGATGAC TCAGAGAGCC TTGCTGATGA TTTTACAATC
    CGTTTGTCAG ATGGGAAACA TAAGATACTT AAAACCATTT CAATAGAGGT CATCCCAGTT
    AATGATGAAA AACCAATGCT GAGCAAGAAG GCTGAAATTG CAATGAATAT GGGTGAAACT
    CGTATTATTT CCAGTGCTAT TCTTTCAGCC ATAGATGAAG ACTCACCCAG GGAGAAGATT
    TACTATGTAT TTGAAAGGCT TCCCCAAAAT GGGCAACTTC AGCTTAAGAT AGGGAGGGAC
    TGGGTTCCTC TCTCCCCTGG CATGAAATGC ACTCAGGAGG AAGTGGATCT GAACTTGCTG
    AGATACACAC ACACCGGGGC AATGGATTCC CAGAATCAAG ATAGCTTCAC CTTCTACCTT
    TGGGATGGCA ACAACAGGTC CCCTGCTCTT GACTGTCAAA TCACCATCAA GGACATGGAA
    AAAGGTGATA TTGTCATCCT TACAAAACCA CTCGTGGTGT CTAAAGGTGA TAGAGGTTTC
    TTAACAACCA CCACTCTCCT GGCTGTGGAC GGAACAGACA AGCCTGAGGA ACTGCTCTAT
    GTCATCACCT CCCCTCCACG ATATGGCCAG ATCGAATATG TTCACTATCC TGGAGTTCCC
    ATTACAAACT TCAGCCAAAT GGATATAGCG GGGCAGACAG TTTGCTATGT ACACAAGAGC
    AAGGTGACTG TCTCCAGTGA CAGATTCAGA TTCATCATCA GCAATGGACT GCGGACCGAG
    CACGGGGTGT TTGAGATCAC ACTGGAGACT GTGGACAGAG CCCTGCCTGT GGTAACCAGG
    AACAAGGGGT TGAGACTGGC CCAAGGGGCC GTGGGCCTGC TTTCCCCTGA CCTCCTTCAG
    CTGACCGACC CTGATACACC TGTGGAGAAC CTCACCTTCC TCTTGGTTCA GCTTCCCCAG
    CATGGCCAGC TCTACCTGTG GGGGACAGGG CTACTTCAAC ACAATTTCAC CCAGCAGGAT
    GTGGACAGCA GGAATGTGGC CTATCGGCAC TCAGGAGGGG ACTCCCAGAC TGACTGCTTT
    ACTTTCATGG CCACAGACGG GACAAACCAA GGCTTTATTG TGAATGGGAG AGTATGGGAA
    GAACCTGTTT TATTCACCAT TCAGGTAGAC CAATTGGACA AAACAGCTCC TCGTATCACA
    CTCTTGCATT CCCCTTCTCA AGTGGGGCTC CTGAAAAATG GCTGCTACGG GATTTACATC
    ACTTCCCGCG TGTTGAAGGC ATCAGACCCT GACACTGAGG ACGATCAGAT CATCTTTAAA
    ATTCTACAAG GCCCAAAACA TGGACATCTG GAGAACACAA CAACAGGTGA ATTTATCCAT
    GAGAAATTTA GCCAAAAGGA CTTAAACAGT AAGACTATTC TTTACATCAT AAACCCATCT
    TTGGAAGTAA ATTCAGATAC CGTGGAATTT CAAATCATGG ACCCCACAGG GAACTCGGCC
    ACTCCTCAAA TTTTGGAACT GAAGTGGTCT CATATTGAAT GGTCACAGAC CGAATATGAA
    GTCTGTGAGA ATGTGGGTGT GTTGCCCTTG GAAATTATCA GAAGGGGATA TTCCATGGAC
    TCGGCCTTTG TGGGTATAAA GGTCAACCAA GTGTCAGCTG CAGATGGAAA AGATTTCACC
    GTGATTCCAT CTAAACTGAT TCAGTTTGAC CCAGGAATGT CAACTAAGAT GTGGAATATA
    GCAATTACCT ATGACGGATT AGAGGAAGAT GATGAGGTCT TTGAAGTAAT TCTGAACTCC
    CCTGTGAATG CAGTTCTTGG CACAAAGACA AAAGCTGCAG TGAAAATTTT GGACTCAAAA
    GGAGGACAAT GCCATCCTTC ATATTCCTCC AACCAAAGCA AGCACAGCAC ATGGGAGAAG
    GGCATTTGGC ATCTGCTGCC CCCAGGGTCT TCCTCATCCA CCACTTCTGG TTCCTTTCAT
    CTGGAAAGAA GACCTCTTCC ATCTTCCATG CAGCTAGCAG TCACCAGGGG AGACACCCTG
    CGGGGCTTTG ATTCTACAGA TCTTTCTCAA AGGAAGCTTA GGACCCGTGG GAATGGCAAA
    ACAGTTCGTC CATCCTCTGT TTATAGAAAT GGAACAGACA TCATCTATAA TTATCATGGG
    ATAGTTTCCT TGAAACTAGA GGATGACAGC TTCCCAACTC ACAAAAGGAA GGCCAAAGTA
    TCCATCATTA GTCAGCCACA AAAGACAATC AAAGTGGCAG AACTGCCTCA AGCAGATAAG
    GTGGAATCCA CAACTGACTC ACACTTCCCC AGACAGGACC AGTTGCCCTC ATTTCCAAAG
    AACTGCACTC TGGAATTAAA GGGACTCTTC CATTTTGAAG AAGGCATCCA GAAGCTGTAT
    CAGTGCAATG GGATTGCCTG GAAAGCCTGG AGTCCCCAAA CCAAGGATGT GGAAGAAAAA
    TCCTGTCCAG CCGGGTGGCA CCAGCACTCA GGCTACTGTC ACATCTTGAT CACAGAGCAG
    AAAGGCACCT GGAATGCGGC TGCCCAAGCT TGCAGGGAAC AATACCTGGG CAACCTTGTA
    ACTGTATTCT CCAGGCAGCA CATGCGGTGG CTCTGGGACA TTGGTGAGAG AAAGTCCTTT
    TGGATAGGTT TGAACGACCA AGTGCATGCT GGCCACTGGG AGTGGATCGG TGGTGAACCT
    GTTGCCTTCA CCAATGGGAG AAGAGGGCCC TCTCAACGCT CCAAGCTTGG AAAGAGCTGT
    GTTTTGGTTC AAAGACAAGG GAAATGGCAA ACAAAAGATT GTAGGAGAGC CAAACCTCAT
    AATTATGTGT GTTCCAGAAA ACTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024201334.1
    CDS1191..7757
    Translation

    Target ORF information:

    RefSeq Version XM_024345566.1
    Organism Pan troglodytes(chimpanzee)
    Definition Pan troglodytes FRAS1 related extracellular matrix 1 (FREM1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024345566.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    ATGAACTCTC TGAGTTGGGG GGCTGCGAAT GCCGTGCTGC TGCTGCTCCT CCTGGCCTGG 
    GCCAGCCCCA CCTTCATCAG CATCAACCGT GGGGTGAGGG TGATGAAGGG CCACTCTGCC
    TTCCTGTCAG GAGATGACCT GAAGTTTGCC ATCCCTAAAG AGAAAGATGC CTGCAAAGTG
    GAAGTTGTGA TGAATGAGCC AATAACCCAG AGGGTTGGGA AACTCACTCC ACAGGTCTTT
    GACTGCCATT TCCTTCCCAA TGAAGTCAAG TATGTTCACA ATGGTTGTCC AATTCTTGAT
    GAAGACACAG TGAAGCTTAG ACTTTACAGA TTTACTGAAA GAGATACCTT CATAGAAACC
    TTTATCCTGT GGGTCTATCT CCTGGAACCA GACTGTAACA TCATCCATAT GAGTAACAAT
    GTGCTGGAGG TGCCTGAATT CAATGGCCTG TCCCAAGCGA TTGATAAAAA TCTGCTCAGA
    TTCGATTATG ATAGGATGGC TAGCCTGGAA TGTACGGTCA GCCTGGACAC TGCGAGAACT
    CGGCTGCCAG CCCATGGCCA GATGGTTCTC GGGGAGCCTC GACCAGAAGA ACCTCGTGGA
    GATCAGCCAC ACAGTTTTTT CCCAGAGTCC CGTATGATGT CTTTTAATGC CTTTCTGGAA
    CTGAGAGCAA AACTGAAGTG TCCAGGTGGA AGCTGTACCC CAGGATTAAA GAAAATAGGA
    AGCCTCAAAG TGAGCTGTGA GGAGTTCCTG CTGATGGGCC TTCGTTATCA GCATCTGGAT
    CCCCCTTCAC CCAACATTGA TTATATCTCC ATCCAACTGG ACCTCACAGA TACCAGGAGT
    AAAATTGTGT ACAAGTCAGA GAGTGCGTGG CTGCCTGTCT ATATCAGAGC TGGAATTCCG
    AATCAGATTC CAAAGGCTGC ATTCATGGCC ATGTTTATTC TGGAAGTGGA TCAGTTCATC
    CTGACCTCCT TGACTACATC AGTTCTGGAC TGTGAAGAAG ATGAGACCCC TAAACCCTTG
    CTGGTGTTCA ACATTACTAA AGCCCCGCTC CAGGGCTATG TGACTCACCT GTTGGATCAC
    ACCAGACCAA TCTCCTCATT CACCTGGAAA GATCTCAGTG ACATGCAGAT CGCCTATCAG
    CCACCAAACA GCAGCCATTC TGAGAGGAGA CATGATGAGG TAGAATTGGA GGTATACGAC
    TTCTTCTTTG AAAGGAGTGC ACCTATGACA GTCCACATCT CCATCAGAAC AGCAGATACA
    AATGCCCCCC GTGTATCCTG GAATACAGGT CTGAATCTCC TTGAGGGGCA GTCTCGAGCC
    ATCACTTGGG AACAGTTTCA GGTTGTCGAC AATGACGACA TCGGTGCTGT CCGGCTAGTC
    ACCGTTGGTG GCCTGCAGCA TGGATGGCTG ACTTTAAGAG GGGGGAAAGG ATTTCTCTTC
    ACCGTGGCTG ACCTCCAGGC TGGAGTTGTT CGCTATCATC ATGATGACAG CGACTCCACC
    AAAGACTTCG TGGTCTTCCG GATATTTGAT GGCCATCACA GCATCCGTCA CAAATTCCCC
    ATCAACGTCT TGCCCAAAGA TGATAGTCCC CCGTTCCTCA TAACCAATGT TGTCATTGAA
    CTGGAGGAGG GGCAGACCAT CCTGATCCAG GGATCCATGC TGCGAGCTTC AGATGTGGAT
    GCCAGTGATG ACTACATCTT CTTCAATATC ACAAAGCCTC CACAGGCTGG GGAGATCATG
    AAGAAGCCAG GGCCAGGACT GATAGGCTAT CCTGTCCCTG GCTTCCTTCA GAGGGATTTG
    TTTAATGGAA TCATTTATTA TCGTCATTTT GGTGGAGAAA TCTTTGAAGA TTCTTTTCAA
    TTTGTCCTGT GGGACAGCCA TGAACCTCCA AATCTTTCAG TGCCACAGGT GGCAACAATC
    CATGTAACTC CAGTGGATGA CCAGCTTCCA AAAGAGGCTC CTGGAGTTTC TCGGCATTTG
    GTTGTCAAGG AAACTGAGGT GGCCTATATA ACTAAGAAAC AGCTACATTT TATAGATTCA
    GAATCATATG ACAGGGAGCT GGTCTACACG ATAACTACTC CTCCATTTTT CTCCTTCAGC
    CACAGACACT TGGATGCTGG GAAATTATTT ATGGTGGACA GCATACCAAA AGTAGTTAAG
    AATCCTACGG CCCTGGAGCT GAGGTCATTC ACTCAGCATG CTGTGAACTA TATGAAAGTG
    GCCTACATGC CCCCCATGCA AGACATTGGT CCCCATTGCA GAGATGTCCA GTTCACATTT
    TCTGTCAGTA ACCAACATGG CGGCACTTTG CATGGGATCT GCTTTAACAT TACAATCCTC
    CCAGTGGACA ATCAAGTTCC TGAGGCGTTC ACCAACCCTC TGAAAGTGAC TGAGGGAGGT
    CAAAGCATCA TCAGCACAGA GCACATTCTG ATTTCTGATG CAGATACCAA GCTGGACAAT
    ATTGACCTCT CCCTGCGGGA ATTGCCTCTG CACGGAAGGG TGGAGCTGAA TGGATTTCCT
    CTAAATTCAG GGGGCACATT TTCTTGGGGC GATCTCCATA CCTTAAAAGT TAGGTATCAA
    CATGATGGAA CTGAAGTTCT TCAGGATGAC CTACTCTTGG AGGTCACCGA TGGCACAAAT
    TCAGCAGAAT TTGTTCTACA TGTTGAGGTA TTCCCTGTCA ACGATGAGCC ACCAGTCTTA
    AAGGCTGACC TCATGCCTGT CATGAATTGC TCAGAGGGAG GAGAGGTGGT CATCACCTCT
    GAATACATTT TTGCTACTGA TGTGGACAGC GATAACTTGA AGTTGATGTT TGTGATTGCT
    CGCGAACCTC AGCATGGGGT GGTGAGGAGA GCTGGAGTCA CAGTGGATCA GTTCTCTCAG
    AGAGATGTTA TCTCAGAGGC CGTGACATAC AAACACACAG GTGGCGAGAT TGGCATGATG
    CCCTGTTTTG ACACCATTAC ATTGGTGGTT TCGGATGGAG AGGCTGGCCC TTTTGTGAAT
    GGCTGTTGCT ACAATGGACC CAATCCATCT GTTCCGCTTC ATGCGTCCTT TCCAGTGTAT
    GATCTCAACA TCACGGTATA CCCAGTAGAC AACCAGCCAC CTTCCATTGC AATAGGTCCC
    ATGTTTGTTG TAGATGAGGG CTGCTCAACA GCCCTTACTG TTAACCATTT GTCCGCCACT
    GACCCTGACA CTGCAGCAGA TGACTTGGAA TTTGTTTTGG TTTCTCCTCC TCAGTTTGGC
    TACCTCGAAA ATATACTCCC TTCTGTGGGT TTTGAAAAAA GCAATATTGG CATAAGTATA
    GATTCATTTC AGTGGAAAGA CATGAACGCT TTTCACATTA ACTATGTGCA GTCCAGGCAT
    CTGAGGATAG AACCAACTGC CGACCAGTTC ACGGTGTACG TCACAGATGG GAAGCAACAC
    TCCTTGGAGA TACCATTTTC TATTATAATC AACCCCACAA ATGATGAAGC TCCTGACTTT
    GTAGTGCAGA ATATTACTGT GTGCGAGGGT CAGATGAAAG AGCTGGACTC TTCCATCATC
    AGCGCTGTGG ACCTGGACAT TCCCCAGGAT GCCCTGCTGT TCAGCATCAC TCAAAAGCCA
    CGCCATGGCC TCCTCATCGA TAGGGGGTTT AGCAAAGACT TCTCTGAGAA TAAGCAGCCA
    GCCAACCCTC ACCAGAAACA TGTACCTGTT CACAGTTTTT CCATGGAACT CCTCAAGACT
    GGAATGAGGT TGACGTACAT GCATGATGAC TCAGAGAGCC TTGCTGATGA TTTTACAATC
    CGTTTGTCAG ATGGGAAACA TAAGATACTT AAAACCATTT CAATAGAGGT CATCCCAGTT
    AATGATGAAA AACCAATGCT GAGCAAGAAG GCTGAAATTG CAATGAATAT GGGTGAAACT
    CGTATTATTT CCAGTGCTAT TCTTTCAGCC ATAGATGAAG ACTCACCCAG GGAGAAGATT
    TACTATGTAT TTGAAAGGCT TCCCCAAAAT GGGCAACTTC AGCTTAAGAT AGGGAGGGAC
    TGGGTTCCTC TCTCCCCTGG CATGAAATGC ACTCAGGAGG AAGTGGATCT GAACTTGCTG
    AGATACACAC ACACCGGGGC AATGGATTCC CAGAATCAAG ATAGCTTCAC CTTCTACCTT
    TGGGATGGCA ACAACAGGTC CCCTGCTCTT GACTGTCAAA TCACCATCAA GGACATGGAA
    AAAGGTGATA TTGTCATCCT TACAAAACCA CTCGTGGTGT CTAAAGGTGA TAGAGGTTTC
    TTAACAACCA CCACTCTCCT GGCTGTGGAC GGAACAGACA AGCCTGAGGA ACTGCTCTAT
    GTCATCACCT CCCCTCCACG ATATGGCCAG ATCGAATATG TTCACTATCC TGGAGTTCCC
    ATTACAAACT TCAGCCAAAT GGATATAGCG GGGCAGACAG TTTGCTATGT ACACAAGAGC
    AAGGTGACTG TCTCCAGTGA CAGATTCAGA TTCATCATCA GCAATGGACT GCGGACCGAG
    CACGGGGTGT TTGAGATCAC ACTGGAGACT GTGGACAGAG CCCTGCCTGT GGTAACCAGG
    AACAAGGGGT TGAGACTGGC CCAAGGGGCC GTGGGCCTGC TTTCCCCTGA CCTCCTTCAG
    CTGACCGACC CTGATACACC TGTGGAGAAC CTCACCTTCC TCTTGGTTCA GCTTCCCCAG
    CATGGCCAGC TCTACCTGTG GGGGACAGGG CTACTTCAAC ACAATTTCAC CCAGCAGGAT
    GTGGACAGCA GGAATGTGGC CTATCGGCAC TCAGGAGGGG ACTCCCAGAC TGACTGCTTT
    ACTTTCATGG CCACAGACGG GACAAACCAA GGCTTTATTG TGAATGGGAG AGTATGGGAA
    GAACCTGTTT TATTCACCAT TCAGGTAGAC CAATTGGACA AAACAGCTCC TCGTATCACA
    CTCTTGCATT CCCCTTCTCA AGTGGGGCTC CTGAAAAATG GCTGCTACGG GATTTACATC
    ACTTCCCGCG TGTTGAAGGC ATCAGACCCT GACACTGAGG ACGATCAGAT CATCTTTAAA
    ATTCTACAAG GCCCAAAACA TGGACATCTG GAGAACACAA CAACAGGTGA ATTTATCCAT
    GAGAAATTTA GCCAAAAGGA CTTAAACAGT AAGACTATTC TTTACATCAT AAACCCATCT
    TTGGAAGTAA ATTCAGATAC CGTGGAATTT CAAATCATGG ACCCCACAGG GAACTCGGCC
    ACTCCTCAAA TTTTGGAACT GAAGTGGTCT CATATTGAAT GGTCACAGAC CGAATATGAA
    GTCTGTGAGA ATGTGGGTGT GTTGCCCTTG GAAATTATCA GAAGGGGATA TTCCATGGAC
    TCGGCCTTTG TGGGTATAAA GGTCAACCAA GTGTCAGCTG CAGATGGAAA AGATTTCACC
    GTGATTCCAT CTAAACTGAT TCAGTTTGAC CCAGGAATGT CAACTAAGAT GTGGAATATA
    GCAATTACCT ATGACGGATT AGAGGAAGAT GATGAGGTCT TTGAAGTAAT TCTGAACTCC
    CCTGTGAATG CAGTTCTTGG CACAAAGACA AAAGCTGCAG TGAAAATTTT GGACTCAAAA
    GGAGGACAAT GCCATCCTTC ATATTCCTCC AACCAAAGCA AGCACAGCAC ATGGGAGAAG
    GGCATTTGGC ATCTGCTGCC CCCAGGGTCT TCCTCATCCA CCACTTCTGG TTCCTTTCAT
    CTGGAAAGAA GACCTCTTCC ATCTTCCATG CAGCTAGCAG TCACCAGGGG AGACACCCTG
    CGGGGCTTTG ATTCTACAGA TCTTTCTCAA AGGAAGCTTA GGACCCGTGG GAATGGCAAA
    ACAGTTCGTC CATCCTCTGT TTATAGAAAT GGAACAGACA TCATCTATAA TTATCATGGG
    ATAGTTTCCT TGAAACTAGA GGATGACAGC TTCCCAACTC ACAAAAGGAA GGCCAAAGTA
    TCCATCATTA GTCAGCCACA AAAGACAATC AAAGTGGCAG AACTGCCTCA AGCAGATAAG
    GTGGAATCCA CAACTGACTC ACACTTCCCC AGACAGGACC AGTTGCCCTC ATTTCCAAAG
    AACTGCACTC TGGAATTAAA GGGACTCTTC CATTTTGAAG AAGGCATCCA GAAGCTGTAT
    CAGTGCAATG GGATTGCCTG GAAAGCCTGG AGTCCCCAAA CCAAGGATGT GGAAGAAAAA
    TCCTGTCCAG CCGGGTGGCA CCAGCACTCA GGCTACTGTC ACATCTTGAT CACAGAGCAG
    AAAGGCACCT GGAATGCGGC TGCCCAAGCT TGCAGGGAAC AATACCTGGG CAACCTTGTA
    ACTGTATTCT CCAGGCAGCA CATGCGGTGG CTCTGGGACA TTGGTGAGAG AAAGTCCTTT
    TGGATAGGTT TGAACGACCA AGTGCATGCT GGCCACTGGG AGTGGATCGG TGGTGAACCT
    GTTGCCTTCA CCAATGGGAG AAGAGGGCCC TCTCAACGCT CCAAGCTTGG AAAGAGCTGT
    GTTTTGGTTC AAAGACAAGG GAAATGGCAA ACAAAAGATT GTAGGAGAGC CAAACCTCAT
    AATTATGTGT GTTCCAGAAA ACTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OPc109242
    Clone ID Related Accession (Same CDS sequence) XM_024345570.1
    Accession Version XM_024345570.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6543bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-03-19
    Organism Pan troglodytes(chimpanzee)
    Product FRAS1-related extracellular matrix protein 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036888.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    ATGAACTCTC TGAGTTGGGG GGCTGCGAAT GCCGTGCTGC TGCTGCTCCT CCTGGCCTGG 
    GCCAGCCCCA CCTTCATCAG CATCAACCGT GGGGTGAGGG TGATGAAGGG CCACTCTGCC
    TTCCTGTCAG GAGATGACCT GAAGTTTGCC ATCCCTAAAG AGAAAGATGC CTGCAAAGTG
    GAAGTTGTGA TGAATGAGCC AATAACCCAG AGGGTTGGGA AACTCACTCC ACAGGTCTTT
    GACTGCCATT TCCTTCCCAA TGAAGTCAAG TATGTTCACA ATGGTTGTCC AATTCTTGAT
    GAAGACACAG TGAAGCTTAG ACTTTACAGA TTTACTGAAA GAGATACCTT CATAGAAACC
    TTTATCCTGT GGGTCTATCT CCTGGAACCA GACTGTAACA TCATCCATAT GAGTAACAAT
    GTGCTGGAGG TGCCTGAATT CAATGGCCTG TCCCAAGCGA TTGATAAAAA TCTGCTCAGA
    TTCGATTATG ATAGGATGGC TAGCCTGGAA TGTACGGTCA GCCTGGACAC TGCGAGAACT
    CGGCTGCCAG CCCATGGCCA GATGGTTCTC GGGGAGCCTC GACCAGAAGA ACCTCGTGGA
    GATCAGCCAC ACAGTTTTTT CCCAGAGTCC CGTATGATGT CTTTTAATGC CTTTCTGGAA
    CTGAGAGCAA AACTGAAGTG TCCAGGTGGA AGCTGTACCC CAGGATTAAA GAAAATAGGA
    AGCCTCAAAG TGAGCTGTGA GGAGTTCCTG CTGATGGGCC TTCGTTATCA GCATCTGGAT
    CCCCCTTCAC CCAACATTGA TTATATCTCC ATCCAACTGG ACCTCACAGA TACCAGGAGT
    AAAATTGTGT ACAAGTCAGA GAGTGCGTGG CTGCCTGTCT ATATCAGAGC TGGAATTCCG
    AATCAGATTC CAAAGGCTGC ATTCATGGCC ATGTTTATTC TGGAAGTGGA TCAGTTCATC
    CTGACCTCCT TGACTACATC AGTTCTGGAC TGTGAAGAAG ATGAGACCCC TAAACCCTTG
    CTGGTGTTCA ACATTACTAA AGCCCCGCTC CAGGGCTATG TGACTCACCT GTTGGATCAC
    ACCAGACCAA TCTCCTCATT CACCTGGAAA GATCTCAGTG ACATGCAGAT CGCCTATCAG
    CCACCAAACA GCAGCCATTC TGAGAGGAGA CATGATGAGG TAGAATTGGA GGTATACGAC
    TTCTTCTTTG AAAGGAGTGC ACCTATGACA GTCCACATCT CCATCAGAAC AGCAGATACA
    AATGCCCCCC GTGTATCCTG GAATACAGGT CTGAATCTCC TTGAGGGGCA GTCTCGAGCC
    ATCACTTGGG AACAGTTTCA GGTTGTCGAC AATGACGACA TCGGTGCTGT CCGGCTAGTC
    ACCGTTGGTG GCCTGCAGCA TGGATGGCTG ACTTTAAGAG GGGGGAAAGG ATTTCTCTTC
    ACCGTGGCTG ACCTCCAGGC TGGAGTTGTT CGCTATCATC ATGATGACAG CGACTCCACC
    AAAGACTTCG TGGTCTTCCG GATATTTGAT GGCCATCACA GCATCCGTCA CAAATTCCCC
    ATCAACGTCT TGCCCAAAGA TGATAGTCCC CCGTTCCTCA TAACCAATGT TGTCATTGAA
    CTGGAGGAGG GGCAGACCAT CCTGATCCAG GGATCCATGC TGCGAGCTTC AGATGTGGAT
    GCCAGTGATG ACTACATCTT CTTCAATATC ACAAAGCCTC CACAGGCTGG GGAGATCATG
    AAGAAGCCAG GGCCAGGACT GATAGGCTAT CCTGTCCCTG GCTTCCTTCA GAGGGATTTG
    TTTAATGGAA TCATTTATTA TCGTCATTTT GGTGGAGAAA TCTTTGAAGA TTCTTTTCAA
    TTTGTCCTGT GGGACAGCCA TGAACCTCCA AATCTTTCAG TGCCACAGGT GGCAACAATC
    CATGTAACTC CAGTGGATGA CCAGCTTCCA AAAGAGGCTC CTGGAGTTTC TCGGCATTTG
    GTTGTCAAGG AAACTGAGGT GGCCTATATA ACTAAGAAAC AGCTACATTT TATAGATTCA
    GAATCATATG ACAGGGAGCT GGTCTACACG ATAACTACTC CTCCATTTTT CTCCTTCAGC
    CACAGACACT TGGATGCTGG GAAATTATTT ATGGTGGACA GCATACCAAA AGTAGTTAAG
    AATCCTACGG CCCTGGAGCT GAGGTCATTC ACTCAGCATG CTGTGAACTA TATGAAAGTG
    GCCTACATGC CCCCCATGCA AGACATTGGT CCCCATTGCA GAGATGTCCA GTTCACATTT
    TCTGTCAGTA ACCAACATGG CGGCACTTTG CATGGGATCT GCTTTAACAT TACAATCCTC
    CCAGTGGACA ATCAAGTTCC TGAGGCGTTC ACCAACCCTC TGAAAGTGAC TGAGGGAGGT
    CAAAGCATCA TCAGCACAGA GCACATTCTG ATTTCTGATG CAGATACCAA GCTGGACAAT
    ATTGACCTCT CCCTGCGGGA ATTGCCTCTG CACGGAAGGG TGGAGCTGAA TGGATTTCCT
    CTAAATTCAG GGGGCACATT TTCTTGGGGC GATCTCCATA CCTTAAAAGT TAGGTATCAA
    CATGATGGAA CTGAAGTTCT TCAGGATGAC CTACTCTTGG AGGTCACCGA TGGCACAAAT
    TCAGCAGAAT TTGTTCTACA TGTTGAGGTA TTCCCTGTCA ACGATGAGCC ACCAGTCTTA
    AAGGCTGACC TCATGCCTGT CATGAATTGC TCAGAGGGAG GAGAGGTGGT CATCACCTCT
    GAATACATTT TTGCTACTGA TGTGGACAGC GATAACTTGA AGTTGATGTT TGTGATTGCT
    CGCGAACCTC AGCATGGGGT GGTGAGGAGA GCTGGAGTCA CAGTGGATCA GTTCTCTCAG
    AGAGATGTTA TCTCAGAGGC CGTGACATAC AAACACACAG GTGGCGAGAT TGGCATGATG
    CCCTGTTTTG ACACCATTAC ATTGGTGGTT TCGGATGGAG AGGCTGGCCC TTTTGTGAAT
    GGCTGTTGCT ACAATGGACC CAATCCATCT GTTCCGCTTC ATGCGTCCTT TCCAGTGTAT
    GATCTCAACA TCACGGTATA CCCAGTAGAC AACCAGCCAC CTTCCATTGC AATAGGTCCC
    ATGTTTGTTG TAGATGAGGG CTGCTCAACA GCCCTTACTG TTAACCATTT GTCCGCCACT
    GACCCTGACA CTGCAGCAGA TGACTTGGAA TTTGTTTTGG TTTCTCCTCC TCAGTTTGGC
    TACCTCGAAA ATATACTCCC TTCTGTGGGT TTTGAAAAAA GCAATATTGG CATAAGTATA
    GATTCATTTC AGTGGAAAGA CATGAACGCT TTTCACATTA ACTATGTGCA GTCCAGGCAT
    CTGAGGATAG AACCAACTGC CGACCAGTTC ACGGTGTACG TCACAGATGG GAAGCAACAC
    TCCTTGGAGA TACCATTTTC TATTATAATC AACCCCACAA ATGATGAAGC TCCTGACTTT
    GTAGTGCAGA ATATTACTGT GTGCGAGGGT CAGATGAAAG AGCTGGACTC TTCCATCATC
    AGCGCTGTGG ACCTGGACAT TCCCCAGGAT GCCCTGCTGT TCAGCATCAC TCAAAAGCCA
    CGCCATGGCC TCCTCATCGA TAGGGGGTTT AGCAAAGACT TCTCTGAGAA TAAGCAGCCA
    GCCAACCCTC ACCAGAAACA TGTACCTGTT CACAGTTTTT CCATGGAACT CCTCAAGACT
    GGAATGAGGT TGACGTACAT GCATGATGAC TCAGAGAGCC TTGCTGATGA TTTTACAATC
    CGTTTGTCAG ATGGGAAACA TAAGATACTT AAAACCATTT CAATAGAGGT CATCCCAGTT
    AATGATGAAA AACCAATGCT GAGCAAGAAG GCTGAAATTG CAATGAATAT GGGTGAAACT
    CGTATTATTT CCAGTGCTAT TCTTTCAGCC ATAGATGAAG ACTCACCCAG GGAGAAGATT
    TACTATGTAT TTGAAAGGCT TCCCCAAAAT GGGCAACTTC AGCTTAAGAT AGGGAGGGAC
    TGGGTTCCTC TCTCCCCTGG CATGAAATGC ACTCAGGAGG AAGTGGATCT GAACTTGCTG
    AGATACACAC ACACCGGGGC AATGGATTCC CAGAATCAAG ATAGCTTCAC CTTCTACCTT
    TGGGATGGCA ACAACAGGTC CCCTGCTCTT GACTGTCAAA TCACCATCAA GGACATGGAA
    AAAGGTGATA TTGTCATCCT TACAAAACCA CTCGTGGTGT CTAAAGGTGA TAGAGGTTTC
    TTAACAACCA CCACTCTCCT GGCTGTGGAC GGAACAGACA AGCCTGAGGA ACTGCTCTAT
    GTCATCACCT CCCCTCCACG ATATGGCCAG ATCGAATATG TTCACTATCC TGGAGTTCCC
    ATTACAAACT TCAGCCAAAT GGATATAGCG GGGCAGACAG TTTGCTATGT ACACAAGAGC
    AAGGTGACTG TCTCCAGTGA CAGATTCAGA TTCATCATCA GCAATGGACT GCGGACCGAG
    CACGGGGTGT TTGAGATCAC ACTGGAGACT GTGGACAGAG CCCTGCCTGT GGTAACCAGG
    AACAAGGGGT TGAGACTGGC CCAAGGGGCC GTGGGCCTGC TTTCCCCTGA CCTCCTTCAG
    CTGACCGACC CTGATACACC TGTGGAGAAC CTCACCTTCC TCTTGGTTCA GCTTCCCCAG
    CATGGCCAGC TCTACCTGTG GGGGACAGGG CTACTTCAAC ACAATTTCAC CCAGCAGGAT
    GTGGACAGCA GGAATGTGGC CTATCGGCAC TCAGGAGGGG ACTCCCAGAC TGACTGCTTT
    ACTTTCATGG CCACAGACGG GACAAACCAA GGCTTTATTG TGAATGGGAG AGTATGGGAA
    GAACCTGTTT TATTCACCAT TCAGGTAGAC CAATTGGACA AAACAGCTCC TCGTATCACA
    CTCTTGCATT CCCCTTCTCA AGTGGGGCTC CTGAAAAATG GCTGCTACGG GATTTACATC
    ACTTCCCGCG TGTTGAAGGC ATCAGACCCT GACACTGAGG ACGATCAGAT CATCTTTAAA
    ATTCTACAAG GCCCAAAACA TGGACATCTG GAGAACACAA CAACAGGTGA ATTTATCCAT
    GAGAAATTTA GCCAAAAGGA CTTAAACAGT AAGACTATTC TTTACATCAT AAACCCATCT
    TTGGAAGTAA ATTCAGATAC CGTGGAATTT CAAATCATGG ACCCCACAGG GAACTCGGCC
    ACTCCTCAAA TTTTGGAACT GAAGTGGTCT CATATTGAAT GGTCACAGAC CGAATATGAA
    GTCTGTGAGA ATGTGGGTGT GTTGCCCTTG GAAATTATCA GAAGGGGATA TTCCATGGAC
    TCGGCCTTTG TGGGTATAAA GGTCAACCAA GTGTCAGCTG CAGATGGAAA AGATTTCACC
    GTGATTCCAT CTAAACTGAT TCAGTTTGAC CCAGGAATGT CAACTAAGAT GTGGAATATA
    GCAATTACCT ATGACGGATT AGAGGAAGAT GATGAGGTCT TTGAAGTAAT TCTGAACTCC
    CCTGTGAATG CAGTTCTTGG CACAAAGACA AAAGCTGCAG TGAAAATTTT GGACTCAAAA
    GGAGGACAAT GCCATCCTTC ATATTCCTCC AACCAAAGCA AGCACAGCAC ATGGGAGAAG
    GGCATTTGGC ATCTGCTGCC CCCAGGGTCT TCCTCATCCA CCACTTCTGG TTCCTTTCAT
    CTGGAAAGAA GACCTCTTCC ATCTTCCATG CAGCTAGCAG TCACCAGGGG AGACACCCTG
    CGGGGCTTTG ATTCTACAGA TCTTTCTCAA AGGAAGCTTA GGACCCGTGG GAATGGCAAA
    ACAGTTCGTC CATCCTCTGT TTATAGAAAT GGAACAGACA TCATCTATAA TTATCATGGG
    ATAGTTTCCT TGAAACTAGA GGATGACAGC TTCCCAACTC ACAAAAGGAA GGCCAAAGTA
    TCCATCATTA GTCAGCCACA AAAGACAATC AAAGTGGCAG AACTGCCTCA AGCAGATAAG
    GTGGAATCCA CAACTGACTC ACACTTCCCC AGACAGGACC AGTTGCCCTC ATTTCCAAAG
    AACTGCACTC TGGAATTAAA GGGACTCTTC CATTTTGAAG AAGGCATCCA GAAGCTGTAT
    CAGTGCAATG GGATTGCCTG GAAAGCCTGG AGTCCCCAAA CCAAGGATGT GGAAGAAAAA
    TCCTGTCCAG CCGGGTGGCA CCAGCACTCA GGCTACTGTC ACATCTTGAT CACAGAGCAG
    AAAGGCACCT GGAATGCGGC TGCCCAAGCT TGCAGGGAAC AATACCTGGG CAACCTTGTA
    ACTGTATTCT CCAGGCAGCA CATGCGGTGG CTCTGGGACA TTGGTTTGAA CGACCAAGTG
    CATGCTGGCC ACTGGGAGTG GATCGGTGGT GAACCTGTTG CCTTCACCAA TGGGAGAAGA
    GGGCCCTCTC AACGCTCCAA GCTTGGAAAG AGCTGTGTTT TGGTTCAAAG ACAAGGGAAA
    TGGCAAACAA AAGATTGTAG GAGAGCCAAA CCTCATAATT ATGTGTGTTC CAGAAAACTC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024201338.1
    CDS1379..7921
    Translation

    Target ORF information:

    RefSeq Version XM_024345570.1
    Organism Pan troglodytes(chimpanzee)
    Definition Pan troglodytes FRAS1 related extracellular matrix 1 (FREM1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024345570.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    ATGAACTCTC TGAGTTGGGG GGCTGCGAAT GCCGTGCTGC TGCTGCTCCT CCTGGCCTGG 
    GCCAGCCCCA CCTTCATCAG CATCAACCGT GGGGTGAGGG TGATGAAGGG CCACTCTGCC
    TTCCTGTCAG GAGATGACCT GAAGTTTGCC ATCCCTAAAG AGAAAGATGC CTGCAAAGTG
    GAAGTTGTGA TGAATGAGCC AATAACCCAG AGGGTTGGGA AACTCACTCC ACAGGTCTTT
    GACTGCCATT TCCTTCCCAA TGAAGTCAAG TATGTTCACA ATGGTTGTCC AATTCTTGAT
    GAAGACACAG TGAAGCTTAG ACTTTACAGA TTTACTGAAA GAGATACCTT CATAGAAACC
    TTTATCCTGT GGGTCTATCT CCTGGAACCA GACTGTAACA TCATCCATAT GAGTAACAAT
    GTGCTGGAGG TGCCTGAATT CAATGGCCTG TCCCAAGCGA TTGATAAAAA TCTGCTCAGA
    TTCGATTATG ATAGGATGGC TAGCCTGGAA TGTACGGTCA GCCTGGACAC TGCGAGAACT
    CGGCTGCCAG CCCATGGCCA GATGGTTCTC GGGGAGCCTC GACCAGAAGA ACCTCGTGGA
    GATCAGCCAC ACAGTTTTTT CCCAGAGTCC CGTATGATGT CTTTTAATGC CTTTCTGGAA
    CTGAGAGCAA AACTGAAGTG TCCAGGTGGA AGCTGTACCC CAGGATTAAA GAAAATAGGA
    AGCCTCAAAG TGAGCTGTGA GGAGTTCCTG CTGATGGGCC TTCGTTATCA GCATCTGGAT
    CCCCCTTCAC CCAACATTGA TTATATCTCC ATCCAACTGG ACCTCACAGA TACCAGGAGT
    AAAATTGTGT ACAAGTCAGA GAGTGCGTGG CTGCCTGTCT ATATCAGAGC TGGAATTCCG
    AATCAGATTC CAAAGGCTGC ATTCATGGCC ATGTTTATTC TGGAAGTGGA TCAGTTCATC
    CTGACCTCCT TGACTACATC AGTTCTGGAC TGTGAAGAAG ATGAGACCCC TAAACCCTTG
    CTGGTGTTCA ACATTACTAA AGCCCCGCTC CAGGGCTATG TGACTCACCT GTTGGATCAC
    ACCAGACCAA TCTCCTCATT CACCTGGAAA GATCTCAGTG ACATGCAGAT CGCCTATCAG
    CCACCAAACA GCAGCCATTC TGAGAGGAGA CATGATGAGG TAGAATTGGA GGTATACGAC
    TTCTTCTTTG AAAGGAGTGC ACCTATGACA GTCCACATCT CCATCAGAAC AGCAGATACA
    AATGCCCCCC GTGTATCCTG GAATACAGGT CTGAATCTCC TTGAGGGGCA GTCTCGAGCC
    ATCACTTGGG AACAGTTTCA GGTTGTCGAC AATGACGACA TCGGTGCTGT CCGGCTAGTC
    ACCGTTGGTG GCCTGCAGCA TGGATGGCTG ACTTTAAGAG GGGGGAAAGG ATTTCTCTTC
    ACCGTGGCTG ACCTCCAGGC TGGAGTTGTT CGCTATCATC ATGATGACAG CGACTCCACC
    AAAGACTTCG TGGTCTTCCG GATATTTGAT GGCCATCACA GCATCCGTCA CAAATTCCCC
    ATCAACGTCT TGCCCAAAGA TGATAGTCCC CCGTTCCTCA TAACCAATGT TGTCATTGAA
    CTGGAGGAGG GGCAGACCAT CCTGATCCAG GGATCCATGC TGCGAGCTTC AGATGTGGAT
    GCCAGTGATG ACTACATCTT CTTCAATATC ACAAAGCCTC CACAGGCTGG GGAGATCATG
    AAGAAGCCAG GGCCAGGACT GATAGGCTAT CCTGTCCCTG GCTTCCTTCA GAGGGATTTG
    TTTAATGGAA TCATTTATTA TCGTCATTTT GGTGGAGAAA TCTTTGAAGA TTCTTTTCAA
    TTTGTCCTGT GGGACAGCCA TGAACCTCCA AATCTTTCAG TGCCACAGGT GGCAACAATC
    CATGTAACTC CAGTGGATGA CCAGCTTCCA AAAGAGGCTC CTGGAGTTTC TCGGCATTTG
    GTTGTCAAGG AAACTGAGGT GGCCTATATA ACTAAGAAAC AGCTACATTT TATAGATTCA
    GAATCATATG ACAGGGAGCT GGTCTACACG ATAACTACTC CTCCATTTTT CTCCTTCAGC
    CACAGACACT TGGATGCTGG GAAATTATTT ATGGTGGACA GCATACCAAA AGTAGTTAAG
    AATCCTACGG CCCTGGAGCT GAGGTCATTC ACTCAGCATG CTGTGAACTA TATGAAAGTG
    GCCTACATGC CCCCCATGCA AGACATTGGT CCCCATTGCA GAGATGTCCA GTTCACATTT
    TCTGTCAGTA ACCAACATGG CGGCACTTTG CATGGGATCT GCTTTAACAT TACAATCCTC
    CCAGTGGACA ATCAAGTTCC TGAGGCGTTC ACCAACCCTC TGAAAGTGAC TGAGGGAGGT
    CAAAGCATCA TCAGCACAGA GCACATTCTG ATTTCTGATG CAGATACCAA GCTGGACAAT
    ATTGACCTCT CCCTGCGGGA ATTGCCTCTG CACGGAAGGG TGGAGCTGAA TGGATTTCCT
    CTAAATTCAG GGGGCACATT TTCTTGGGGC GATCTCCATA CCTTAAAAGT TAGGTATCAA
    CATGATGGAA CTGAAGTTCT TCAGGATGAC CTACTCTTGG AGGTCACCGA TGGCACAAAT
    TCAGCAGAAT TTGTTCTACA TGTTGAGGTA TTCCCTGTCA ACGATGAGCC ACCAGTCTTA
    AAGGCTGACC TCATGCCTGT CATGAATTGC TCAGAGGGAG GAGAGGTGGT CATCACCTCT
    GAATACATTT TTGCTACTGA TGTGGACAGC GATAACTTGA AGTTGATGTT TGTGATTGCT
    CGCGAACCTC AGCATGGGGT GGTGAGGAGA GCTGGAGTCA CAGTGGATCA GTTCTCTCAG
    AGAGATGTTA TCTCAGAGGC CGTGACATAC AAACACACAG GTGGCGAGAT TGGCATGATG
    CCCTGTTTTG ACACCATTAC ATTGGTGGTT TCGGATGGAG AGGCTGGCCC TTTTGTGAAT
    GGCTGTTGCT ACAATGGACC CAATCCATCT GTTCCGCTTC ATGCGTCCTT TCCAGTGTAT
    GATCTCAACA TCACGGTATA CCCAGTAGAC AACCAGCCAC CTTCCATTGC AATAGGTCCC
    ATGTTTGTTG TAGATGAGGG CTGCTCAACA GCCCTTACTG TTAACCATTT GTCCGCCACT
    GACCCTGACA CTGCAGCAGA TGACTTGGAA TTTGTTTTGG TTTCTCCTCC TCAGTTTGGC
    TACCTCGAAA ATATACTCCC TTCTGTGGGT TTTGAAAAAA GCAATATTGG CATAAGTATA
    GATTCATTTC AGTGGAAAGA CATGAACGCT TTTCACATTA ACTATGTGCA GTCCAGGCAT
    CTGAGGATAG AACCAACTGC CGACCAGTTC ACGGTGTACG TCACAGATGG GAAGCAACAC
    TCCTTGGAGA TACCATTTTC TATTATAATC AACCCCACAA ATGATGAAGC TCCTGACTTT
    GTAGTGCAGA ATATTACTGT GTGCGAGGGT CAGATGAAAG AGCTGGACTC TTCCATCATC
    AGCGCTGTGG ACCTGGACAT TCCCCAGGAT GCCCTGCTGT TCAGCATCAC TCAAAAGCCA
    CGCCATGGCC TCCTCATCGA TAGGGGGTTT AGCAAAGACT TCTCTGAGAA TAAGCAGCCA
    GCCAACCCTC ACCAGAAACA TGTACCTGTT CACAGTTTTT CCATGGAACT CCTCAAGACT
    GGAATGAGGT TGACGTACAT GCATGATGAC TCAGAGAGCC TTGCTGATGA TTTTACAATC
    CGTTTGTCAG ATGGGAAACA TAAGATACTT AAAACCATTT CAATAGAGGT CATCCCAGTT
    AATGATGAAA AACCAATGCT GAGCAAGAAG GCTGAAATTG CAATGAATAT GGGTGAAACT
    CGTATTATTT CCAGTGCTAT TCTTTCAGCC ATAGATGAAG ACTCACCCAG GGAGAAGATT
    TACTATGTAT TTGAAAGGCT TCCCCAAAAT GGGCAACTTC AGCTTAAGAT AGGGAGGGAC
    TGGGTTCCTC TCTCCCCTGG CATGAAATGC ACTCAGGAGG AAGTGGATCT GAACTTGCTG
    AGATACACAC ACACCGGGGC AATGGATTCC CAGAATCAAG ATAGCTTCAC CTTCTACCTT
    TGGGATGGCA ACAACAGGTC CCCTGCTCTT GACTGTCAAA TCACCATCAA GGACATGGAA
    AAAGGTGATA TTGTCATCCT TACAAAACCA CTCGTGGTGT CTAAAGGTGA TAGAGGTTTC
    TTAACAACCA CCACTCTCCT GGCTGTGGAC GGAACAGACA AGCCTGAGGA ACTGCTCTAT
    GTCATCACCT CCCCTCCACG ATATGGCCAG ATCGAATATG TTCACTATCC TGGAGTTCCC
    ATTACAAACT TCAGCCAAAT GGATATAGCG GGGCAGACAG TTTGCTATGT ACACAAGAGC
    AAGGTGACTG TCTCCAGTGA CAGATTCAGA TTCATCATCA GCAATGGACT GCGGACCGAG
    CACGGGGTGT TTGAGATCAC ACTGGAGACT GTGGACAGAG CCCTGCCTGT GGTAACCAGG
    AACAAGGGGT TGAGACTGGC CCAAGGGGCC GTGGGCCTGC TTTCCCCTGA CCTCCTTCAG
    CTGACCGACC CTGATACACC TGTGGAGAAC CTCACCTTCC TCTTGGTTCA GCTTCCCCAG
    CATGGCCAGC TCTACCTGTG GGGGACAGGG CTACTTCAAC ACAATTTCAC CCAGCAGGAT
    GTGGACAGCA GGAATGTGGC CTATCGGCAC TCAGGAGGGG ACTCCCAGAC TGACTGCTTT
    ACTTTCATGG CCACAGACGG GACAAACCAA GGCTTTATTG TGAATGGGAG AGTATGGGAA
    GAACCTGTTT TATTCACCAT TCAGGTAGAC CAATTGGACA AAACAGCTCC TCGTATCACA
    CTCTTGCATT CCCCTTCTCA AGTGGGGCTC CTGAAAAATG GCTGCTACGG GATTTACATC
    ACTTCCCGCG TGTTGAAGGC ATCAGACCCT GACACTGAGG ACGATCAGAT CATCTTTAAA
    ATTCTACAAG GCCCAAAACA TGGACATCTG GAGAACACAA CAACAGGTGA ATTTATCCAT
    GAGAAATTTA GCCAAAAGGA CTTAAACAGT AAGACTATTC TTTACATCAT AAACCCATCT
    TTGGAAGTAA ATTCAGATAC CGTGGAATTT CAAATCATGG ACCCCACAGG GAACTCGGCC
    ACTCCTCAAA TTTTGGAACT GAAGTGGTCT CATATTGAAT GGTCACAGAC CGAATATGAA
    GTCTGTGAGA ATGTGGGTGT GTTGCCCTTG GAAATTATCA GAAGGGGATA TTCCATGGAC
    TCGGCCTTTG TGGGTATAAA GGTCAACCAA GTGTCAGCTG CAGATGGAAA AGATTTCACC
    GTGATTCCAT CTAAACTGAT TCAGTTTGAC CCAGGAATGT CAACTAAGAT GTGGAATATA
    GCAATTACCT ATGACGGATT AGAGGAAGAT GATGAGGTCT TTGAAGTAAT TCTGAACTCC
    CCTGTGAATG CAGTTCTTGG CACAAAGACA AAAGCTGCAG TGAAAATTTT GGACTCAAAA
    GGAGGACAAT GCCATCCTTC ATATTCCTCC AACCAAAGCA AGCACAGCAC ATGGGAGAAG
    GGCATTTGGC ATCTGCTGCC CCCAGGGTCT TCCTCATCCA CCACTTCTGG TTCCTTTCAT
    CTGGAAAGAA GACCTCTTCC ATCTTCCATG CAGCTAGCAG TCACCAGGGG AGACACCCTG
    CGGGGCTTTG ATTCTACAGA TCTTTCTCAA AGGAAGCTTA GGACCCGTGG GAATGGCAAA
    ACAGTTCGTC CATCCTCTGT TTATAGAAAT GGAACAGACA TCATCTATAA TTATCATGGG
    ATAGTTTCCT TGAAACTAGA GGATGACAGC TTCCCAACTC ACAAAAGGAA GGCCAAAGTA
    TCCATCATTA GTCAGCCACA AAAGACAATC AAAGTGGCAG AACTGCCTCA AGCAGATAAG
    GTGGAATCCA CAACTGACTC ACACTTCCCC AGACAGGACC AGTTGCCCTC ATTTCCAAAG
    AACTGCACTC TGGAATTAAA GGGACTCTTC CATTTTGAAG AAGGCATCCA GAAGCTGTAT
    CAGTGCAATG GGATTGCCTG GAAAGCCTGG AGTCCCCAAA CCAAGGATGT GGAAGAAAAA
    TCCTGTCCAG CCGGGTGGCA CCAGCACTCA GGCTACTGTC ACATCTTGAT CACAGAGCAG
    AAAGGCACCT GGAATGCGGC TGCCCAAGCT TGCAGGGAAC AATACCTGGG CAACCTTGTA
    ACTGTATTCT CCAGGCAGCA CATGCGGTGG CTCTGGGACA TTGGTTTGAA CGACCAAGTG
    CATGCTGGCC ACTGGGAGTG GATCGGTGGT GAACCTGTTG CCTTCACCAA TGGGAGAAGA
    GGGCCCTCTC AACGCTCCAA GCTTGGAAAG AGCTGTGTTT TGGTTCAAAG ACAAGGGAAA
    TGGCAAACAA AAGATTGTAG GAGAGCCAAA CCTCATAATT ATGTGTGTTC CAGAAAACTC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OPc109243
    Clone ID Related Accession (Same CDS sequence) XM_009456318.3 , XM_024345571.1
    Accession Version XM_009456318.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6540bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-03-19
    Organism Pan troglodytes(chimpanzee)
    Product FRAS1-related extracellular matrix protein 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036888.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_009456318.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGAACTCTC TGAGTTGGGG GGCTGCGAAT GCCGTGCTGC TGCTGCTCCT CCTGGCCTGG 
    GCCAGCCCCA CCTTCATCAG CATCAACCGT GGGGTGAGGG TGATGAAGGG CCACTCTGCC
    TTCCTGTCAG GAGATGACCT GAAGTTTGCC ATCCCTAAAG AGAAAGATGC CTGCAAAGTG
    GAAGTTGTGA TGAATGAGCC AATAACCCAG AGGGTTGGGA AACTCACTCC ACAGGTCTTT
    GACTGCCATT TCCTTCCCAA TGAAGTCAAG TATGTTCACA ATGGTTGTCC AATTCTTGAT
    GAAGACACAG TGAAGCTTAG ACTTTACAGA TTTACTGAAA GAGATACCTT CATAGAAACC
    TTTATCCTGT GGGTCTATCT CCTGGAACCA GACTGTAACA TCATCCATAT GAGTAACAAT
    GTGCTGGAGG TGCCTGAATT CAATGGCCTG TCCCAAGCGA TTGATAAAAA TCTGCTCAGA
    TTCGATTATG ATAGGATGGC TAGCCTGGAA TGTACGGTCA GCCTGGACAC TGCGAGAACT
    CGGCTGCCAG CCCATGGCCA GATGGTTCTC GGGGAGCCTC GACCAGAAGA ACCTCGTGGA
    GATCAGCCAC ACAGTTTTTT CCCAGAGTCC CAACTGAGAG CAAAACTGAA GTGTCCAGGT
    GGAAGCTGTA CCCCAGGATT AAAGAAAATA GGAAGCCTCA AAGTGAGCTG TGAGGAGTTC
    CTGCTGATGG GCCTTCGTTA TCAGCATCTG GATCCCCCTT CACCCAACAT TGATTATATC
    TCCATCCAAC TGGACCTCAC AGATACCAGG AGTAAAATTG TGTACAAGTC AGAGAGTGCG
    TGGCTGCCTG TCTATATCAG AGCTGGAATT CCGAATCAGA TTCCAAAGGC TGCATTCATG
    GCCATGTTTA TTCTGGAAGT GGATCAGTTC ATCCTGACCT CCTTGACTAC ATCAGTTCTG
    GACTGTGAAG AAGATGAGAC CCCTAAACCC TTGCTGGTGT TCAACATTAC TAAAGCCCCG
    CTCCAGGGCT ATGTGACTCA CCTGTTGGAT CACACCAGAC CAATCTCCTC ATTCACCTGG
    AAAGATCTCA GTGACATGCA GATCGCCTAT CAGCCACCAA ACAGCAGCCA TTCTGAGAGG
    AGACATGATG AGGTAGAATT GGAGGTATAC GACTTCTTCT TTGAAAGGAG TGCACCTATG
    ACAGTCCACA TCTCCATCAG AACAGCAGAT ACAAATGCCC CCCGTGTATC CTGGAATACA
    GGTCTGAATC TCCTTGAGGG GCAGTCTCGA GCCATCACTT GGGAACAGTT TCAGGTTGTC
    GACAATGACG ACATCGGTGC TGTCCGGCTA GTCACCGTTG GTGGCCTGCA GCATGGATGG
    CTGACTTTAA GAGGGGGGAA AGGATTTCTC TTCACCGTGG CTGACCTCCA GGCTGGAGTT
    GTTCGCTATC ATCATGATGA CAGCGACTCC ACCAAAGACT TCGTGGTCTT CCGGATATTT
    GATGGCCATC ACAGCATCCG TCACAAATTC CCCATCAACG TCTTGCCCAA AGATGATAGT
    CCCCCGTTCC TCATAACCAA TGTTGTCATT GAACTGGAGG AGGGGCAGAC CATCCTGATC
    CAGGGATCCA TGCTGCGAGC TTCAGATGTG GATGCCAGTG ATGACTACAT CTTCTTCAAT
    ATCACAAAGC CTCCACAGGC TGGGGAGATC ATGAAGAAGC CAGGGCCAGG ACTGATAGGC
    TATCCTGTCC CTGGCTTCCT TCAGAGGGAT TTGTTTAATG GAATCATTTA TTATCGTCAT
    TTTGGTGGAG AAATCTTTGA AGATTCTTTT CAATTTGTCC TGTGGGACAG CCATGAACCT
    CCAAATCTTT CAGTGCCACA GGTGGCAACA ATCCATGTAA CTCCAGTGGA TGACCAGCTT
    CCAAAAGAGG CTCCTGGAGT TTCTCGGCAT TTGGTTGTCA AGGAAACTGA GGTGGCCTAT
    ATAACTAAGA AACAGCTACA TTTTATAGAT TCAGAATCAT ATGACAGGGA GCTGGTCTAC
    ACGATAACTA CTCCTCCATT TTTCTCCTTC AGCCACAGAC ACTTGGATGC TGGGAAATTA
    TTTATGGTGG ACAGCATACC AAAAGTAGTT AAGAATCCTA CGGCCCTGGA GCTGAGGTCA
    TTCACTCAGC ATGCTGTGAA CTATATGAAA GTGGCCTACA TGCCCCCCAT GCAAGACATT
    GGTCCCCATT GCAGAGATGT CCAGTTCACA TTTTCTGTCA GTAACCAACA TGGCGGCACT
    TTGCATGGGA TCTGCTTTAA CATTACAATC CTCCCAGTGG ACAATCAAGT TCCTGAGGCG
    TTCACCAACC CTCTGAAAGT GACTGAGGGA GGTCAAAGCA TCATCAGCAC AGAGCACATT
    CTGATTTCTG ATGCAGATAC CAAGCTGGAC AATATTGACC TCTCCCTGCG GGAATTGCCT
    CTGCACGGAA GGGTGGAGCT GAATGGATTT CCTCTAAATT CAGGGGGCAC ATTTTCTTGG
    GGCGATCTCC ATACCTTAAA AGTTAGGTAT CAACATGATG GAACTGAAGT TCTTCAGGAT
    GACCTACTCT TGGAGGTCAC CGATGGCACA AATTCAGCAG AATTTGTTCT ACATGTTGAG
    GTATTCCCTG TCAACGATGA GCCACCAGTC TTAAAGGCTG ACCTCATGCC TGTCATGAAT
    TGCTCAGAGG GAGGAGAGGT GGTCATCACC TCTGAATACA TTTTTGCTAC TGATGTGGAC
    AGCGATAACT TGAAGTTGAT GTTTGTGATT GCTCGCGAAC CTCAGCATGG GGTGGTGAGG
    AGAGCTGGAG TCACAGTGGA TCAGTTCTCT CAGAGAGATG TTATCTCAGA GGCCGTGACA
    TACAAACACA CAGGTGGCGA GATTGGCATG ATGCCCTGTT TTGACACCAT TACATTGGTG
    GTTTCGGATG GAGAGGCTGG CCCTTTTGTG AATGGCTGTT GCTACAATGG ACCCAATCCA
    TCTGTTCCGC TTCATGCGTC CTTTCCAGTG TATGATCTCA ACATCACGGT ATACCCAGTA
    GACAACCAGC CACCTTCCAT TGCAATAGGT CCCATGTTTG TTGTAGATGA GGGCTGCTCA
    ACAGCCCTTA CTGTTAACCA TTTGTCCGCC ACTGACCCTG ACACTGCAGC AGATGACTTG
    GAATTTGTTT TGGTTTCTCC TCCTCAGTTT GGCTACCTCG AAAATATACT CCCTTCTGTG
    GGTTTTGAAA AAAGCAATAT TGGCATAAGT ATAGATTCAT TTCAGTGGAA AGACATGAAC
    GCTTTTCACA TTAACTATGT GCAGTCCAGG CATCTGAGGA TAGAACCAAC TGCCGACCAG
    TTCACGGTGT ACGTCACAGA TGGGAAGCAA CACTCCTTGG AGATACCATT TTCTATTATA
    ATCAACCCCA CAAATGATGA AGCTCCTGAC TTTGTAGTGC AGAATATTAC TGTGTGCGAG
    GGTCAGATGA AAGAGCTGGA CTCTTCCATC ATCAGCGCTG TGGACCTGGA CATTCCCCAG
    GATGCCCTGC TGTTCAGCAT CACTCAAAAG CCACGCCATG GCCTCCTCAT CGATAGGGGG
    TTTAGCAAAG ACTTCTCTGA GAATAAGCAG CCAGCCAACC CTCACCAGAA ACATGTACCT
    GTTCACAGTT TTTCCATGGA ACTCCTCAAG ACTGGAATGA GGTTGACGTA CATGCATGAT
    GACTCAGAGA GCCTTGCTGA TGATTTTACA ATCCGTTTGT CAGATGGGAA ACATAAGATA
    CTTAAAACCA TTTCAATAGA GGTCATCCCA GTTAATGATG AAAAACCAAT GCTGAGCAAG
    AAGGCTGAAA TTGCAATGAA TATGGGTGAA ACTCGTATTA TTTCCAGTGC TATTCTTTCA
    GCCATAGATG AAGACTCACC CAGGGAGAAG ATTTACTATG TATTTGAAAG GCTTCCCCAA
    AATGGGCAAC TTCAGCTTAA GATAGGGAGG GACTGGGTTC CTCTCTCCCC TGGCATGAAA
    TGCACTCAGG AGGAAGTGGA TCTGAACTTG CTGAGATACA CACACACCGG GGCAATGGAT
    TCCCAGAATC AAGATAGCTT CACCTTCTAC CTTTGGGATG GCAACAACAG GTCCCCTGCT
    CTTGACTGTC AAATCACCAT CAAGGACATG GAAAAAGGTG ATATTGTCAT CCTTACAAAA
    CCACTCGTGG TGTCTAAAGG TGATAGAGGT TTCTTAACAA CCACCACTCT CCTGGCTGTG
    GACGGAACAG ACAAGCCTGA GGAACTGCTC TATGTCATCA CCTCCCCTCC ACGATATGGC
    CAGATCGAAT ATGTTCACTA TCCTGGAGTT CCCATTACAA ACTTCAGCCA AATGGATATA
    GCGGGGCAGA CAGTTTGCTA TGTACACAAG AGCAAGGTGA CTGTCTCCAG TGACAGATTC
    AGATTCATCA TCAGCAATGG ACTGCGGACC GAGCACGGGG TGTTTGAGAT CACACTGGAG
    ACTGTGGACA GAGCCCTGCC TGTGGTAACC AGGAACAAGG GGTTGAGACT GGCCCAAGGG
    GCCGTGGGCC TGCTTTCCCC TGACCTCCTT CAGCTGACCG ACCCTGATAC ACCTGTGGAG
    AACCTCACCT TCCTCTTGGT TCAGCTTCCC CAGCATGGCC AGCTCTACCT GTGGGGGACA
    GGGCTACTTC AACACAATTT CACCCAGCAG GATGTGGACA GCAGGAATGT GGCCTATCGG
    CACTCAGGAG GGGACTCCCA GACTGACTGC TTTACTTTCA TGGCCACAGA CGGGACAAAC
    CAAGGCTTTA TTGTGAATGG GAGAGTATGG GAAGAACCTG TTTTATTCAC CATTCAGGTA
    GACCAATTGG ACAAAACAGC TCCTCGTATC ACACTCTTGC ATTCCCCTTC TCAAGTGGGG
    CTCCTGAAAA ATGGCTGCTA CGGGATTTAC ATCACTTCCC GCGTGTTGAA GGCATCAGAC
    CCTGACACTG AGGACGATCA GATCATCTTT AAAATTCTAC AAGGCCCAAA ACATGGACAT
    CTGGAGAACA CAACAACAGG TGAATTTATC CATGAGAAAT TTAGCCAAAA GGACTTAAAC
    AGTAAGACTA TTCTTTACAT CATAAACCCA TCTTTGGAAG TAAATTCAGA TACCGTGGAA
    TTTCAAATCA TGGACCCCAC AGGGAACTCG GCCACTCCTC AAATTTTGGA ACTGAAGTGG
    TCTCATATTG AATGGTCACA GACCGAATAT GAAGTCTGTG AGAATGTGGG TGTGTTGCCC
    TTGGAAATTA TCAGAAGGGG ATATTCCATG GACTCGGCCT TTGTGGGTAT AAAGGTCAAC
    CAAGTGTCAG CTGCAGATGG AAAAGATTTC ACCGTGATTC CATCTAAACT GATTCAGTTT
    GACCCAGGAA TGTCAACTAA GATGTGGAAT ATAGCAATTA CCTATGACGG ATTAGAGGAA
    GATGATGAGG TCTTTGAAGT AATTCTGAAC TCCCCTGTGA ATGCAGTTCT TGGCACAAAG
    ACAAAAGCTG CAGTGAAAAT TTTGGACTCA AAAGGAGGAC AATGCCATCC TTCATATTCC
    TCCAACCAAA GCAAGCACAG CACATGGGAG AAGGGCATTT GGCATCTGCT GCCCCCAGGG
    TCTTCCTCAT CCACCACTTC TGGTTCCTTT CATCTGGAAA GAAGACCTCT TCCATCTTCC
    ATGCAGCTAG CAGTCACCAG GGGAGACACC CTGCGGGGCT TTGATTCTAC AGATCTTTCT
    CAAAGGAAGC TTAGGACCCG TGGGAATGGC AAAACAGTTC GTCCATCCTC TGTTTATAGA
    AATGGAACAG ACATCATCTA TAATTATCAT GGGATAGTTT CCTTGAAACT AGAGGATGAC
    AGCTTCCCAA CTCACAAAAG GAAGGCCAAA GTATCCATCA TTAGTCAGCC ACAAAAGACA
    ATCAAAGTGG CAGAACTGCC TCAAGCAGAT AAGGTGGAAT CCACAACTGA CTCACACTTC
    CCCAGACAGG ACCAGTTGCC CTCATTTCCA AAGAACTGCA CTCTGGAATT AAAGGGACTC
    TTCCATTTTG AAGAAGGCAT CCAGAAGCTG TATCAGTGCA ATGGGATTGC CTGGAAAGCC
    TGGAGTCCCC AAACCAAGGA TGTGGAAGAA AAATCCTGTC CAGCCGGGTG GCACCAGCAC
    TCAGGCTACT GTCACATCTT GATCACAGAG CAGAAAGGCA CCTGGAATGC GGCTGCCCAA
    GCTTGCAGGG AACAATACCT GGGCAACCTT GTAACTGTAT TCTCCAGGCA GCACATGCGG
    TGGCTCTGGG ACATTGGTGA GAGAAAGTCC TTTTGGATAG GTTTGAACGA CCAAGTGCAT
    GCTGGCCACT GGGAGTGGAT CGGTGGTGAA CCTGTTGCCT TCACCAATGG GAGAAGAGGG
    CCCTCTCAAC GCTCCAAGCT TGGAAAGAGC TGTGTTTTGG TTCAAAGACA AGGGAAATGG
    CAAACAAAAG ATTGTAGGAG AGCCAAACCT CATAATTATG TGTGTTCCAG AAAACTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009454593.3
    CDS1062..7601
    Translation

    Target ORF information:

    RefSeq Version XM_009456318.3
    Organism Pan troglodytes(chimpanzee)
    Definition Pan troglodytes FRAS1 related extracellular matrix 1 (FREM1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009456318.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGAACTCTC TGAGTTGGGG GGCTGCGAAT GCCGTGCTGC TGCTGCTCCT CCTGGCCTGG 
    GCCAGCCCCA CCTTCATCAG CATCAACCGT GGGGTGAGGG TGATGAAGGG CCACTCTGCC
    TTCCTGTCAG GAGATGACCT GAAGTTTGCC ATCCCTAAAG AGAAAGATGC CTGCAAAGTG
    GAAGTTGTGA TGAATGAGCC AATAACCCAG AGGGTTGGGA AACTCACTCC ACAGGTCTTT
    GACTGCCATT TCCTTCCCAA TGAAGTCAAG TATGTTCACA ATGGTTGTCC AATTCTTGAT
    GAAGACACAG TGAAGCTTAG ACTTTACAGA TTTACTGAAA GAGATACCTT CATAGAAACC
    TTTATCCTGT GGGTCTATCT CCTGGAACCA GACTGTAACA TCATCCATAT GAGTAACAAT
    GTGCTGGAGG TGCCTGAATT CAATGGCCTG TCCCAAGCGA TTGATAAAAA TCTGCTCAGA
    TTCGATTATG ATAGGATGGC TAGCCTGGAA TGTACGGTCA GCCTGGACAC TGCGAGAACT
    CGGCTGCCAG CCCATGGCCA GATGGTTCTC GGGGAGCCTC GACCAGAAGA ACCTCGTGGA
    GATCAGCCAC ACAGTTTTTT CCCAGAGTCC CAACTGAGAG CAAAACTGAA GTGTCCAGGT
    GGAAGCTGTA CCCCAGGATT AAAGAAAATA GGAAGCCTCA AAGTGAGCTG TGAGGAGTTC
    CTGCTGATGG GCCTTCGTTA TCAGCATCTG GATCCCCCTT CACCCAACAT TGATTATATC
    TCCATCCAAC TGGACCTCAC AGATACCAGG AGTAAAATTG TGTACAAGTC AGAGAGTGCG
    TGGCTGCCTG TCTATATCAG AGCTGGAATT CCGAATCAGA TTCCAAAGGC TGCATTCATG
    GCCATGTTTA TTCTGGAAGT GGATCAGTTC ATCCTGACCT CCTTGACTAC ATCAGTTCTG
    GACTGTGAAG AAGATGAGAC CCCTAAACCC TTGCTGGTGT TCAACATTAC TAAAGCCCCG
    CTCCAGGGCT ATGTGACTCA CCTGTTGGAT CACACCAGAC CAATCTCCTC ATTCACCTGG
    AAAGATCTCA GTGACATGCA GATCGCCTAT CAGCCACCAA ACAGCAGCCA TTCTGAGAGG
    AGACATGATG AGGTAGAATT GGAGGTATAC GACTTCTTCT TTGAAAGGAG TGCACCTATG
    ACAGTCCACA TCTCCATCAG AACAGCAGAT ACAAATGCCC CCCGTGTATC CTGGAATACA
    GGTCTGAATC TCCTTGAGGG GCAGTCTCGA GCCATCACTT GGGAACAGTT TCAGGTTGTC
    GACAATGACG ACATCGGTGC TGTCCGGCTA GTCACCGTTG GTGGCCTGCA GCATGGATGG
    CTGACTTTAA GAGGGGGGAA AGGATTTCTC TTCACCGTGG CTGACCTCCA GGCTGGAGTT
    GTTCGCTATC ATCATGATGA CAGCGACTCC ACCAAAGACT TCGTGGTCTT CCGGATATTT
    GATGGCCATC ACAGCATCCG TCACAAATTC CCCATCAACG TCTTGCCCAA AGATGATAGT
    CCCCCGTTCC TCATAACCAA TGTTGTCATT GAACTGGAGG AGGGGCAGAC CATCCTGATC
    CAGGGATCCA TGCTGCGAGC TTCAGATGTG GATGCCAGTG ATGACTACAT CTTCTTCAAT
    ATCACAAAGC CTCCACAGGC TGGGGAGATC ATGAAGAAGC CAGGGCCAGG ACTGATAGGC
    TATCCTGTCC CTGGCTTCCT TCAGAGGGAT TTGTTTAATG GAATCATTTA TTATCGTCAT
    TTTGGTGGAG AAATCTTTGA AGATTCTTTT CAATTTGTCC TGTGGGACAG CCATGAACCT
    CCAAATCTTT CAGTGCCACA GGTGGCAACA ATCCATGTAA CTCCAGTGGA TGACCAGCTT
    CCAAAAGAGG CTCCTGGAGT TTCTCGGCAT TTGGTTGTCA AGGAAACTGA GGTGGCCTAT
    ATAACTAAGA AACAGCTACA TTTTATAGAT TCAGAATCAT ATGACAGGGA GCTGGTCTAC
    ACGATAACTA CTCCTCCATT TTTCTCCTTC AGCCACAGAC ACTTGGATGC TGGGAAATTA
    TTTATGGTGG ACAGCATACC AAAAGTAGTT AAGAATCCTA CGGCCCTGGA GCTGAGGTCA
    TTCACTCAGC ATGCTGTGAA CTATATGAAA GTGGCCTACA TGCCCCCCAT GCAAGACATT
    GGTCCCCATT GCAGAGATGT CCAGTTCACA TTTTCTGTCA GTAACCAACA TGGCGGCACT
    TTGCATGGGA TCTGCTTTAA CATTACAATC CTCCCAGTGG ACAATCAAGT TCCTGAGGCG
    TTCACCAACC CTCTGAAAGT GACTGAGGGA GGTCAAAGCA TCATCAGCAC AGAGCACATT
    CTGATTTCTG ATGCAGATAC CAAGCTGGAC AATATTGACC TCTCCCTGCG GGAATTGCCT
    CTGCACGGAA GGGTGGAGCT GAATGGATTT CCTCTAAATT CAGGGGGCAC ATTTTCTTGG
    GGCGATCTCC ATACCTTAAA AGTTAGGTAT CAACATGATG GAACTGAAGT TCTTCAGGAT
    GACCTACTCT TGGAGGTCAC CGATGGCACA AATTCAGCAG AATTTGTTCT ACATGTTGAG
    GTATTCCCTG TCAACGATGA GCCACCAGTC TTAAAGGCTG ACCTCATGCC TGTCATGAAT
    TGCTCAGAGG GAGGAGAGGT GGTCATCACC TCTGAATACA TTTTTGCTAC TGATGTGGAC
    AGCGATAACT TGAAGTTGAT GTTTGTGATT GCTCGCGAAC CTCAGCATGG GGTGGTGAGG
    AGAGCTGGAG TCACAGTGGA TCAGTTCTCT CAGAGAGATG TTATCTCAGA GGCCGTGACA
    TACAAACACA CAGGTGGCGA GATTGGCATG ATGCCCTGTT TTGACACCAT TACATTGGTG
    GTTTCGGATG GAGAGGCTGG CCCTTTTGTG AATGGCTGTT GCTACAATGG ACCCAATCCA
    TCTGTTCCGC TTCATGCGTC CTTTCCAGTG TATGATCTCA ACATCACGGT ATACCCAGTA
    GACAACCAGC CACCTTCCAT TGCAATAGGT CCCATGTTTG TTGTAGATGA GGGCTGCTCA
    ACAGCCCTTA CTGTTAACCA TTTGTCCGCC ACTGACCCTG ACACTGCAGC AGATGACTTG
    GAATTTGTTT TGGTTTCTCC TCCTCAGTTT GGCTACCTCG AAAATATACT CCCTTCTGTG
    GGTTTTGAAA AAAGCAATAT TGGCATAAGT ATAGATTCAT TTCAGTGGAA AGACATGAAC
    GCTTTTCACA TTAACTATGT GCAGTCCAGG CATCTGAGGA TAGAACCAAC TGCCGACCAG
    TTCACGGTGT ACGTCACAGA TGGGAAGCAA CACTCCTTGG AGATACCATT TTCTATTATA
    ATCAACCCCA CAAATGATGA AGCTCCTGAC TTTGTAGTGC AGAATATTAC TGTGTGCGAG
    GGTCAGATGA AAGAGCTGGA CTCTTCCATC ATCAGCGCTG TGGACCTGGA CATTCCCCAG
    GATGCCCTGC TGTTCAGCAT CACTCAAAAG CCACGCCATG GCCTCCTCAT CGATAGGGGG
    TTTAGCAAAG ACTTCTCTGA GAATAAGCAG CCAGCCAACC CTCACCAGAA ACATGTACCT
    GTTCACAGTT TTTCCATGGA ACTCCTCAAG ACTGGAATGA GGTTGACGTA CATGCATGAT
    GACTCAGAGA GCCTTGCTGA TGATTTTACA ATCCGTTTGT CAGATGGGAA ACATAAGATA
    CTTAAAACCA TTTCAATAGA GGTCATCCCA GTTAATGATG AAAAACCAAT GCTGAGCAAG
    AAGGCTGAAA TTGCAATGAA TATGGGTGAA ACTCGTATTA TTTCCAGTGC TATTCTTTCA
    GCCATAGATG AAGACTCACC CAGGGAGAAG ATTTACTATG TATTTGAAAG GCTTCCCCAA
    AATGGGCAAC TTCAGCTTAA GATAGGGAGG GACTGGGTTC CTCTCTCCCC TGGCATGAAA
    TGCACTCAGG AGGAAGTGGA TCTGAACTTG CTGAGATACA CACACACCGG GGCAATGGAT
    TCCCAGAATC AAGATAGCTT CACCTTCTAC CTTTGGGATG GCAACAACAG GTCCCCTGCT
    CTTGACTGTC AAATCACCAT CAAGGACATG GAAAAAGGTG ATATTGTCAT CCTTACAAAA
    CCACTCGTGG TGTCTAAAGG TGATAGAGGT TTCTTAACAA CCACCACTCT CCTGGCTGTG
    GACGGAACAG ACAAGCCTGA GGAACTGCTC TATGTCATCA CCTCCCCTCC ACGATATGGC
    CAGATCGAAT ATGTTCACTA TCCTGGAGTT CCCATTACAA ACTTCAGCCA AATGGATATA
    GCGGGGCAGA CAGTTTGCTA TGTACACAAG AGCAAGGTGA CTGTCTCCAG TGACAGATTC
    AGATTCATCA TCAGCAATGG ACTGCGGACC GAGCACGGGG TGTTTGAGAT CACACTGGAG
    ACTGTGGACA GAGCCCTGCC TGTGGTAACC AGGAACAAGG GGTTGAGACT GGCCCAAGGG
    GCCGTGGGCC TGCTTTCCCC TGACCTCCTT CAGCTGACCG ACCCTGATAC ACCTGTGGAG
    AACCTCACCT TCCTCTTGGT TCAGCTTCCC CAGCATGGCC AGCTCTACCT GTGGGGGACA
    GGGCTACTTC AACACAATTT CACCCAGCAG GATGTGGACA GCAGGAATGT GGCCTATCGG
    CACTCAGGAG GGGACTCCCA GACTGACTGC TTTACTTTCA TGGCCACAGA CGGGACAAAC
    CAAGGCTTTA TTGTGAATGG GAGAGTATGG GAAGAACCTG TTTTATTCAC CATTCAGGTA
    GACCAATTGG ACAAAACAGC TCCTCGTATC ACACTCTTGC ATTCCCCTTC TCAAGTGGGG
    CTCCTGAAAA ATGGCTGCTA CGGGATTTAC ATCACTTCCC GCGTGTTGAA GGCATCAGAC
    CCTGACACTG AGGACGATCA GATCATCTTT AAAATTCTAC AAGGCCCAAA ACATGGACAT
    CTGGAGAACA CAACAACAGG TGAATTTATC CATGAGAAAT TTAGCCAAAA GGACTTAAAC
    AGTAAGACTA TTCTTTACAT CATAAACCCA TCTTTGGAAG TAAATTCAGA TACCGTGGAA
    TTTCAAATCA TGGACCCCAC AGGGAACTCG GCCACTCCTC AAATTTTGGA ACTGAAGTGG
    TCTCATATTG AATGGTCACA GACCGAATAT GAAGTCTGTG AGAATGTGGG TGTGTTGCCC
    TTGGAAATTA TCAGAAGGGG ATATTCCATG GACTCGGCCT TTGTGGGTAT AAAGGTCAAC
    CAAGTGTCAG CTGCAGATGG AAAAGATTTC ACCGTGATTC CATCTAAACT GATTCAGTTT
    GACCCAGGAA TGTCAACTAA GATGTGGAAT ATAGCAATTA CCTATGACGG ATTAGAGGAA
    GATGATGAGG TCTTTGAAGT AATTCTGAAC TCCCCTGTGA ATGCAGTTCT TGGCACAAAG
    ACAAAAGCTG CAGTGAAAAT TTTGGACTCA AAAGGAGGAC AATGCCATCC TTCATATTCC
    TCCAACCAAA GCAAGCACAG CACATGGGAG AAGGGCATTT GGCATCTGCT GCCCCCAGGG
    TCTTCCTCAT CCACCACTTC TGGTTCCTTT CATCTGGAAA GAAGACCTCT TCCATCTTCC
    ATGCAGCTAG CAGTCACCAG GGGAGACACC CTGCGGGGCT TTGATTCTAC AGATCTTTCT
    CAAAGGAAGC TTAGGACCCG TGGGAATGGC AAAACAGTTC GTCCATCCTC TGTTTATAGA
    AATGGAACAG ACATCATCTA TAATTATCAT GGGATAGTTT CCTTGAAACT AGAGGATGAC
    AGCTTCCCAA CTCACAAAAG GAAGGCCAAA GTATCCATCA TTAGTCAGCC ACAAAAGACA
    ATCAAAGTGG CAGAACTGCC TCAAGCAGAT AAGGTGGAAT CCACAACTGA CTCACACTTC
    CCCAGACAGG ACCAGTTGCC CTCATTTCCA AAGAACTGCA CTCTGGAATT AAAGGGACTC
    TTCCATTTTG AAGAAGGCAT CCAGAAGCTG TATCAGTGCA ATGGGATTGC CTGGAAAGCC
    TGGAGTCCCC AAACCAAGGA TGTGGAAGAA AAATCCTGTC CAGCCGGGTG GCACCAGCAC
    TCAGGCTACT GTCACATCTT GATCACAGAG CAGAAAGGCA CCTGGAATGC GGCTGCCCAA
    GCTTGCAGGG AACAATACCT GGGCAACCTT GTAACTGTAT TCTCCAGGCA GCACATGCGG
    TGGCTCTGGG ACATTGGTGA GAGAAAGTCC TTTTGGATAG GTTTGAACGA CCAAGTGCAT
    GCTGGCCACT GGGAGTGGAT CGGTGGTGAA CCTGTTGCCT TCACCAATGG GAGAAGAGGG
    CCCTCTCAAC GCTCCAAGCT TGGAAAGAGC TGTGTTTTGG TTCAAAGACA AGGGAAATGG
    CAAACAAAAG ATTGTAGGAG AGCCAAACCT CATAATTATG TGTGTTCCAG AAAACTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OPc109243
    Clone ID Related Accession (Same CDS sequence) XM_009456318.3 , XM_024345571.1
    Accession Version XM_024345571.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6540bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-03-19
    Organism Pan troglodytes(chimpanzee)
    Product FRAS1-related extracellular matrix protein 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036888.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGAACTCTC TGAGTTGGGG GGCTGCGAAT GCCGTGCTGC TGCTGCTCCT CCTGGCCTGG 
    GCCAGCCCCA CCTTCATCAG CATCAACCGT GGGGTGAGGG TGATGAAGGG CCACTCTGCC
    TTCCTGTCAG GAGATGACCT GAAGTTTGCC ATCCCTAAAG AGAAAGATGC CTGCAAAGTG
    GAAGTTGTGA TGAATGAGCC AATAACCCAG AGGGTTGGGA AACTCACTCC ACAGGTCTTT
    GACTGCCATT TCCTTCCCAA TGAAGTCAAG TATGTTCACA ATGGTTGTCC AATTCTTGAT
    GAAGACACAG TGAAGCTTAG ACTTTACAGA TTTACTGAAA GAGATACCTT CATAGAAACC
    TTTATCCTGT GGGTCTATCT CCTGGAACCA GACTGTAACA TCATCCATAT GAGTAACAAT
    GTGCTGGAGG TGCCTGAATT CAATGGCCTG TCCCAAGCGA TTGATAAAAA TCTGCTCAGA
    TTCGATTATG ATAGGATGGC TAGCCTGGAA TGTACGGTCA GCCTGGACAC TGCGAGAACT
    CGGCTGCCAG CCCATGGCCA GATGGTTCTC GGGGAGCCTC GACCAGAAGA ACCTCGTGGA
    GATCAGCCAC ACAGTTTTTT CCCAGAGTCC CAACTGAGAG CAAAACTGAA GTGTCCAGGT
    GGAAGCTGTA CCCCAGGATT AAAGAAAATA GGAAGCCTCA AAGTGAGCTG TGAGGAGTTC
    CTGCTGATGG GCCTTCGTTA TCAGCATCTG GATCCCCCTT CACCCAACAT TGATTATATC
    TCCATCCAAC TGGACCTCAC AGATACCAGG AGTAAAATTG TGTACAAGTC AGAGAGTGCG
    TGGCTGCCTG TCTATATCAG AGCTGGAATT CCGAATCAGA TTCCAAAGGC TGCATTCATG
    GCCATGTTTA TTCTGGAAGT GGATCAGTTC ATCCTGACCT CCTTGACTAC ATCAGTTCTG
    GACTGTGAAG AAGATGAGAC CCCTAAACCC TTGCTGGTGT TCAACATTAC TAAAGCCCCG
    CTCCAGGGCT ATGTGACTCA CCTGTTGGAT CACACCAGAC CAATCTCCTC ATTCACCTGG
    AAAGATCTCA GTGACATGCA GATCGCCTAT CAGCCACCAA ACAGCAGCCA TTCTGAGAGG
    AGACATGATG AGGTAGAATT GGAGGTATAC GACTTCTTCT TTGAAAGGAG TGCACCTATG
    ACAGTCCACA TCTCCATCAG AACAGCAGAT ACAAATGCCC CCCGTGTATC CTGGAATACA
    GGTCTGAATC TCCTTGAGGG GCAGTCTCGA GCCATCACTT GGGAACAGTT TCAGGTTGTC
    GACAATGACG ACATCGGTGC TGTCCGGCTA GTCACCGTTG GTGGCCTGCA GCATGGATGG
    CTGACTTTAA GAGGGGGGAA AGGATTTCTC TTCACCGTGG CTGACCTCCA GGCTGGAGTT
    GTTCGCTATC ATCATGATGA CAGCGACTCC ACCAAAGACT TCGTGGTCTT CCGGATATTT
    GATGGCCATC ACAGCATCCG TCACAAATTC CCCATCAACG TCTTGCCCAA AGATGATAGT
    CCCCCGTTCC TCATAACCAA TGTTGTCATT GAACTGGAGG AGGGGCAGAC CATCCTGATC
    CAGGGATCCA TGCTGCGAGC TTCAGATGTG GATGCCAGTG ATGACTACAT CTTCTTCAAT
    ATCACAAAGC CTCCACAGGC TGGGGAGATC ATGAAGAAGC CAGGGCCAGG ACTGATAGGC
    TATCCTGTCC CTGGCTTCCT TCAGAGGGAT TTGTTTAATG GAATCATTTA TTATCGTCAT
    TTTGGTGGAG AAATCTTTGA AGATTCTTTT CAATTTGTCC TGTGGGACAG CCATGAACCT
    CCAAATCTTT CAGTGCCACA GGTGGCAACA ATCCATGTAA CTCCAGTGGA TGACCAGCTT
    CCAAAAGAGG CTCCTGGAGT TTCTCGGCAT TTGGTTGTCA AGGAAACTGA GGTGGCCTAT
    ATAACTAAGA AACAGCTACA TTTTATAGAT TCAGAATCAT ATGACAGGGA GCTGGTCTAC
    ACGATAACTA CTCCTCCATT TTTCTCCTTC AGCCACAGAC ACTTGGATGC TGGGAAATTA
    TTTATGGTGG ACAGCATACC AAAAGTAGTT AAGAATCCTA CGGCCCTGGA GCTGAGGTCA
    TTCACTCAGC ATGCTGTGAA CTATATGAAA GTGGCCTACA TGCCCCCCAT GCAAGACATT
    GGTCCCCATT GCAGAGATGT CCAGTTCACA TTTTCTGTCA GTAACCAACA TGGCGGCACT
    TTGCATGGGA TCTGCTTTAA CATTACAATC CTCCCAGTGG ACAATCAAGT TCCTGAGGCG
    TTCACCAACC CTCTGAAAGT GACTGAGGGA GGTCAAAGCA TCATCAGCAC AGAGCACATT
    CTGATTTCTG ATGCAGATAC CAAGCTGGAC AATATTGACC TCTCCCTGCG GGAATTGCCT
    CTGCACGGAA GGGTGGAGCT GAATGGATTT CCTCTAAATT CAGGGGGCAC ATTTTCTTGG
    GGCGATCTCC ATACCTTAAA AGTTAGGTAT CAACATGATG GAACTGAAGT TCTTCAGGAT
    GACCTACTCT TGGAGGTCAC CGATGGCACA AATTCAGCAG AATTTGTTCT ACATGTTGAG
    GTATTCCCTG TCAACGATGA GCCACCAGTC TTAAAGGCTG ACCTCATGCC TGTCATGAAT
    TGCTCAGAGG GAGGAGAGGT GGTCATCACC TCTGAATACA TTTTTGCTAC TGATGTGGAC
    AGCGATAACT TGAAGTTGAT GTTTGTGATT GCTCGCGAAC CTCAGCATGG GGTGGTGAGG
    AGAGCTGGAG TCACAGTGGA TCAGTTCTCT CAGAGAGATG TTATCTCAGA GGCCGTGACA
    TACAAACACA CAGGTGGCGA GATTGGCATG ATGCCCTGTT TTGACACCAT TACATTGGTG
    GTTTCGGATG GAGAGGCTGG CCCTTTTGTG AATGGCTGTT GCTACAATGG ACCCAATCCA
    TCTGTTCCGC TTCATGCGTC CTTTCCAGTG TATGATCTCA ACATCACGGT ATACCCAGTA
    GACAACCAGC CACCTTCCAT TGCAATAGGT CCCATGTTTG TTGTAGATGA GGGCTGCTCA
    ACAGCCCTTA CTGTTAACCA TTTGTCCGCC ACTGACCCTG ACACTGCAGC AGATGACTTG
    GAATTTGTTT TGGTTTCTCC TCCTCAGTTT GGCTACCTCG AAAATATACT CCCTTCTGTG
    GGTTTTGAAA AAAGCAATAT TGGCATAAGT ATAGATTCAT TTCAGTGGAA AGACATGAAC
    GCTTTTCACA TTAACTATGT GCAGTCCAGG CATCTGAGGA TAGAACCAAC TGCCGACCAG
    TTCACGGTGT ACGTCACAGA TGGGAAGCAA CACTCCTTGG AGATACCATT TTCTATTATA
    ATCAACCCCA CAAATGATGA AGCTCCTGAC TTTGTAGTGC AGAATATTAC TGTGTGCGAG
    GGTCAGATGA AAGAGCTGGA CTCTTCCATC ATCAGCGCTG TGGACCTGGA CATTCCCCAG
    GATGCCCTGC TGTTCAGCAT CACTCAAAAG CCACGCCATG GCCTCCTCAT CGATAGGGGG
    TTTAGCAAAG ACTTCTCTGA GAATAAGCAG CCAGCCAACC CTCACCAGAA ACATGTACCT
    GTTCACAGTT TTTCCATGGA ACTCCTCAAG ACTGGAATGA GGTTGACGTA CATGCATGAT
    GACTCAGAGA GCCTTGCTGA TGATTTTACA ATCCGTTTGT CAGATGGGAA ACATAAGATA
    CTTAAAACCA TTTCAATAGA GGTCATCCCA GTTAATGATG AAAAACCAAT GCTGAGCAAG
    AAGGCTGAAA TTGCAATGAA TATGGGTGAA ACTCGTATTA TTTCCAGTGC TATTCTTTCA
    GCCATAGATG AAGACTCACC CAGGGAGAAG ATTTACTATG TATTTGAAAG GCTTCCCCAA
    AATGGGCAAC TTCAGCTTAA GATAGGGAGG GACTGGGTTC CTCTCTCCCC TGGCATGAAA
    TGCACTCAGG AGGAAGTGGA TCTGAACTTG CTGAGATACA CACACACCGG GGCAATGGAT
    TCCCAGAATC AAGATAGCTT CACCTTCTAC CTTTGGGATG GCAACAACAG GTCCCCTGCT
    CTTGACTGTC AAATCACCAT CAAGGACATG GAAAAAGGTG ATATTGTCAT CCTTACAAAA
    CCACTCGTGG TGTCTAAAGG TGATAGAGGT TTCTTAACAA CCACCACTCT CCTGGCTGTG
    GACGGAACAG ACAAGCCTGA GGAACTGCTC TATGTCATCA CCTCCCCTCC ACGATATGGC
    CAGATCGAAT ATGTTCACTA TCCTGGAGTT CCCATTACAA ACTTCAGCCA AATGGATATA
    GCGGGGCAGA CAGTTTGCTA TGTACACAAG AGCAAGGTGA CTGTCTCCAG TGACAGATTC
    AGATTCATCA TCAGCAATGG ACTGCGGACC GAGCACGGGG TGTTTGAGAT CACACTGGAG
    ACTGTGGACA GAGCCCTGCC TGTGGTAACC AGGAACAAGG GGTTGAGACT GGCCCAAGGG
    GCCGTGGGCC TGCTTTCCCC TGACCTCCTT CAGCTGACCG ACCCTGATAC ACCTGTGGAG
    AACCTCACCT TCCTCTTGGT TCAGCTTCCC CAGCATGGCC AGCTCTACCT GTGGGGGACA
    GGGCTACTTC AACACAATTT CACCCAGCAG GATGTGGACA GCAGGAATGT GGCCTATCGG
    CACTCAGGAG GGGACTCCCA GACTGACTGC TTTACTTTCA TGGCCACAGA CGGGACAAAC
    CAAGGCTTTA TTGTGAATGG GAGAGTATGG GAAGAACCTG TTTTATTCAC CATTCAGGTA
    GACCAATTGG ACAAAACAGC TCCTCGTATC ACACTCTTGC ATTCCCCTTC TCAAGTGGGG
    CTCCTGAAAA ATGGCTGCTA CGGGATTTAC ATCACTTCCC GCGTGTTGAA GGCATCAGAC
    CCTGACACTG AGGACGATCA GATCATCTTT AAAATTCTAC AAGGCCCAAA ACATGGACAT
    CTGGAGAACA CAACAACAGG TGAATTTATC CATGAGAAAT TTAGCCAAAA GGACTTAAAC
    AGTAAGACTA TTCTTTACAT CATAAACCCA TCTTTGGAAG TAAATTCAGA TACCGTGGAA
    TTTCAAATCA TGGACCCCAC AGGGAACTCG GCCACTCCTC AAATTTTGGA ACTGAAGTGG
    TCTCATATTG AATGGTCACA GACCGAATAT GAAGTCTGTG AGAATGTGGG TGTGTTGCCC
    TTGGAAATTA TCAGAAGGGG ATATTCCATG GACTCGGCCT TTGTGGGTAT AAAGGTCAAC
    CAAGTGTCAG CTGCAGATGG AAAAGATTTC ACCGTGATTC CATCTAAACT GATTCAGTTT
    GACCCAGGAA TGTCAACTAA GATGTGGAAT ATAGCAATTA CCTATGACGG ATTAGAGGAA
    GATGATGAGG TCTTTGAAGT AATTCTGAAC TCCCCTGTGA ATGCAGTTCT TGGCACAAAG
    ACAAAAGCTG CAGTGAAAAT TTTGGACTCA AAAGGAGGAC AATGCCATCC TTCATATTCC
    TCCAACCAAA GCAAGCACAG CACATGGGAG AAGGGCATTT GGCATCTGCT GCCCCCAGGG
    TCTTCCTCAT CCACCACTTC TGGTTCCTTT CATCTGGAAA GAAGACCTCT TCCATCTTCC
    ATGCAGCTAG CAGTCACCAG GGGAGACACC CTGCGGGGCT TTGATTCTAC AGATCTTTCT
    CAAAGGAAGC TTAGGACCCG TGGGAATGGC AAAACAGTTC GTCCATCCTC TGTTTATAGA
    AATGGAACAG ACATCATCTA TAATTATCAT GGGATAGTTT CCTTGAAACT AGAGGATGAC
    AGCTTCCCAA CTCACAAAAG GAAGGCCAAA GTATCCATCA TTAGTCAGCC ACAAAAGACA
    ATCAAAGTGG CAGAACTGCC TCAAGCAGAT AAGGTGGAAT CCACAACTGA CTCACACTTC
    CCCAGACAGG ACCAGTTGCC CTCATTTCCA AAGAACTGCA CTCTGGAATT AAAGGGACTC
    TTCCATTTTG AAGAAGGCAT CCAGAAGCTG TATCAGTGCA ATGGGATTGC CTGGAAAGCC
    TGGAGTCCCC AAACCAAGGA TGTGGAAGAA AAATCCTGTC CAGCCGGGTG GCACCAGCAC
    TCAGGCTACT GTCACATCTT GATCACAGAG CAGAAAGGCA CCTGGAATGC GGCTGCCCAA
    GCTTGCAGGG AACAATACCT GGGCAACCTT GTAACTGTAT TCTCCAGGCA GCACATGCGG
    TGGCTCTGGG ACATTGGTGA GAGAAAGTCC TTTTGGATAG GTTTGAACGA CCAAGTGCAT
    GCTGGCCACT GGGAGTGGAT CGGTGGTGAA CCTGTTGCCT TCACCAATGG GAGAAGAGGG
    CCCTCTCAAC GCTCCAAGCT TGGAAAGAGC TGTGTTTTGG TTCAAAGACA AGGGAAATGG
    CAAACAAAAG ATTGTAGGAG AGCCAAACCT CATAATTATG TGTGTTCCAG AAAACTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024201339.1
    CDS1379..7918
    Translation

    Target ORF information:

    RefSeq Version XM_024345571.1
    Organism Pan troglodytes(chimpanzee)
    Definition Pan troglodytes FRAS1 related extracellular matrix 1 (FREM1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024345571.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGAACTCTC TGAGTTGGGG GGCTGCGAAT GCCGTGCTGC TGCTGCTCCT CCTGGCCTGG 
    GCCAGCCCCA CCTTCATCAG CATCAACCGT GGGGTGAGGG TGATGAAGGG CCACTCTGCC
    TTCCTGTCAG GAGATGACCT GAAGTTTGCC ATCCCTAAAG AGAAAGATGC CTGCAAAGTG
    GAAGTTGTGA TGAATGAGCC AATAACCCAG AGGGTTGGGA AACTCACTCC ACAGGTCTTT
    GACTGCCATT TCCTTCCCAA TGAAGTCAAG TATGTTCACA ATGGTTGTCC AATTCTTGAT
    GAAGACACAG TGAAGCTTAG ACTTTACAGA TTTACTGAAA GAGATACCTT CATAGAAACC
    TTTATCCTGT GGGTCTATCT CCTGGAACCA GACTGTAACA TCATCCATAT GAGTAACAAT
    GTGCTGGAGG TGCCTGAATT CAATGGCCTG TCCCAAGCGA TTGATAAAAA TCTGCTCAGA
    TTCGATTATG ATAGGATGGC TAGCCTGGAA TGTACGGTCA GCCTGGACAC TGCGAGAACT
    CGGCTGCCAG CCCATGGCCA GATGGTTCTC GGGGAGCCTC GACCAGAAGA ACCTCGTGGA
    GATCAGCCAC ACAGTTTTTT CCCAGAGTCC CAACTGAGAG CAAAACTGAA GTGTCCAGGT
    GGAAGCTGTA CCCCAGGATT AAAGAAAATA GGAAGCCTCA AAGTGAGCTG TGAGGAGTTC
    CTGCTGATGG GCCTTCGTTA TCAGCATCTG GATCCCCCTT CACCCAACAT TGATTATATC
    TCCATCCAAC TGGACCTCAC AGATACCAGG AGTAAAATTG TGTACAAGTC AGAGAGTGCG
    TGGCTGCCTG TCTATATCAG AGCTGGAATT CCGAATCAGA TTCCAAAGGC TGCATTCATG
    GCCATGTTTA TTCTGGAAGT GGATCAGTTC ATCCTGACCT CCTTGACTAC ATCAGTTCTG
    GACTGTGAAG AAGATGAGAC CCCTAAACCC TTGCTGGTGT TCAACATTAC TAAAGCCCCG
    CTCCAGGGCT ATGTGACTCA CCTGTTGGAT CACACCAGAC CAATCTCCTC ATTCACCTGG
    AAAGATCTCA GTGACATGCA GATCGCCTAT CAGCCACCAA ACAGCAGCCA TTCTGAGAGG
    AGACATGATG AGGTAGAATT GGAGGTATAC GACTTCTTCT TTGAAAGGAG TGCACCTATG
    ACAGTCCACA TCTCCATCAG AACAGCAGAT ACAAATGCCC CCCGTGTATC CTGGAATACA
    GGTCTGAATC TCCTTGAGGG GCAGTCTCGA GCCATCACTT GGGAACAGTT TCAGGTTGTC
    GACAATGACG ACATCGGTGC TGTCCGGCTA GTCACCGTTG GTGGCCTGCA GCATGGATGG
    CTGACTTTAA GAGGGGGGAA AGGATTTCTC TTCACCGTGG CTGACCTCCA GGCTGGAGTT
    GTTCGCTATC ATCATGATGA CAGCGACTCC ACCAAAGACT TCGTGGTCTT CCGGATATTT
    GATGGCCATC ACAGCATCCG TCACAAATTC CCCATCAACG TCTTGCCCAA AGATGATAGT
    CCCCCGTTCC TCATAACCAA TGTTGTCATT GAACTGGAGG AGGGGCAGAC CATCCTGATC
    CAGGGATCCA TGCTGCGAGC TTCAGATGTG GATGCCAGTG ATGACTACAT CTTCTTCAAT
    ATCACAAAGC CTCCACAGGC TGGGGAGATC ATGAAGAAGC CAGGGCCAGG ACTGATAGGC
    TATCCTGTCC CTGGCTTCCT TCAGAGGGAT TTGTTTAATG GAATCATTTA TTATCGTCAT
    TTTGGTGGAG AAATCTTTGA AGATTCTTTT CAATTTGTCC TGTGGGACAG CCATGAACCT
    CCAAATCTTT CAGTGCCACA GGTGGCAACA ATCCATGTAA CTCCAGTGGA TGACCAGCTT
    CCAAAAGAGG CTCCTGGAGT TTCTCGGCAT TTGGTTGTCA AGGAAACTGA GGTGGCCTAT
    ATAACTAAGA AACAGCTACA TTTTATAGAT TCAGAATCAT ATGACAGGGA GCTGGTCTAC
    ACGATAACTA CTCCTCCATT TTTCTCCTTC AGCCACAGAC ACTTGGATGC TGGGAAATTA
    TTTATGGTGG ACAGCATACC AAAAGTAGTT AAGAATCCTA CGGCCCTGGA GCTGAGGTCA
    TTCACTCAGC ATGCTGTGAA CTATATGAAA GTGGCCTACA TGCCCCCCAT GCAAGACATT
    GGTCCCCATT GCAGAGATGT CCAGTTCACA TTTTCTGTCA GTAACCAACA TGGCGGCACT
    TTGCATGGGA TCTGCTTTAA CATTACAATC CTCCCAGTGG ACAATCAAGT TCCTGAGGCG
    TTCACCAACC CTCTGAAAGT GACTGAGGGA GGTCAAAGCA TCATCAGCAC AGAGCACATT
    CTGATTTCTG ATGCAGATAC CAAGCTGGAC AATATTGACC TCTCCCTGCG GGAATTGCCT
    CTGCACGGAA GGGTGGAGCT GAATGGATTT CCTCTAAATT CAGGGGGCAC ATTTTCTTGG
    GGCGATCTCC ATACCTTAAA AGTTAGGTAT CAACATGATG GAACTGAAGT TCTTCAGGAT
    GACCTACTCT TGGAGGTCAC CGATGGCACA AATTCAGCAG AATTTGTTCT ACATGTTGAG
    GTATTCCCTG TCAACGATGA GCCACCAGTC TTAAAGGCTG ACCTCATGCC TGTCATGAAT
    TGCTCAGAGG GAGGAGAGGT GGTCATCACC TCTGAATACA TTTTTGCTAC TGATGTGGAC
    AGCGATAACT TGAAGTTGAT GTTTGTGATT GCTCGCGAAC CTCAGCATGG GGTGGTGAGG
    AGAGCTGGAG TCACAGTGGA TCAGTTCTCT CAGAGAGATG TTATCTCAGA GGCCGTGACA
    TACAAACACA CAGGTGGCGA GATTGGCATG ATGCCCTGTT TTGACACCAT TACATTGGTG
    GTTTCGGATG GAGAGGCTGG CCCTTTTGTG AATGGCTGTT GCTACAATGG ACCCAATCCA
    TCTGTTCCGC TTCATGCGTC CTTTCCAGTG TATGATCTCA ACATCACGGT ATACCCAGTA
    GACAACCAGC CACCTTCCAT TGCAATAGGT CCCATGTTTG TTGTAGATGA GGGCTGCTCA
    ACAGCCCTTA CTGTTAACCA TTTGTCCGCC ACTGACCCTG ACACTGCAGC AGATGACTTG
    GAATTTGTTT TGGTTTCTCC TCCTCAGTTT GGCTACCTCG AAAATATACT CCCTTCTGTG
    GGTTTTGAAA AAAGCAATAT TGGCATAAGT ATAGATTCAT TTCAGTGGAA AGACATGAAC
    GCTTTTCACA TTAACTATGT GCAGTCCAGG CATCTGAGGA TAGAACCAAC TGCCGACCAG
    TTCACGGTGT ACGTCACAGA TGGGAAGCAA CACTCCTTGG AGATACCATT TTCTATTATA
    ATCAACCCCA CAAATGATGA AGCTCCTGAC TTTGTAGTGC AGAATATTAC TGTGTGCGAG
    GGTCAGATGA AAGAGCTGGA CTCTTCCATC ATCAGCGCTG TGGACCTGGA CATTCCCCAG
    GATGCCCTGC TGTTCAGCAT CACTCAAAAG CCACGCCATG GCCTCCTCAT CGATAGGGGG
    TTTAGCAAAG ACTTCTCTGA GAATAAGCAG CCAGCCAACC CTCACCAGAA ACATGTACCT
    GTTCACAGTT TTTCCATGGA ACTCCTCAAG ACTGGAATGA GGTTGACGTA CATGCATGAT
    GACTCAGAGA GCCTTGCTGA TGATTTTACA ATCCGTTTGT CAGATGGGAA ACATAAGATA
    CTTAAAACCA TTTCAATAGA GGTCATCCCA GTTAATGATG AAAAACCAAT GCTGAGCAAG
    AAGGCTGAAA TTGCAATGAA TATGGGTGAA ACTCGTATTA TTTCCAGTGC TATTCTTTCA
    GCCATAGATG AAGACTCACC CAGGGAGAAG ATTTACTATG TATTTGAAAG GCTTCCCCAA
    AATGGGCAAC TTCAGCTTAA GATAGGGAGG GACTGGGTTC CTCTCTCCCC TGGCATGAAA
    TGCACTCAGG AGGAAGTGGA TCTGAACTTG CTGAGATACA CACACACCGG GGCAATGGAT
    TCCCAGAATC AAGATAGCTT CACCTTCTAC CTTTGGGATG GCAACAACAG GTCCCCTGCT
    CTTGACTGTC AAATCACCAT CAAGGACATG GAAAAAGGTG ATATTGTCAT CCTTACAAAA
    CCACTCGTGG TGTCTAAAGG TGATAGAGGT TTCTTAACAA CCACCACTCT CCTGGCTGTG
    GACGGAACAG ACAAGCCTGA GGAACTGCTC TATGTCATCA CCTCCCCTCC ACGATATGGC
    CAGATCGAAT ATGTTCACTA TCCTGGAGTT CCCATTACAA ACTTCAGCCA AATGGATATA
    GCGGGGCAGA CAGTTTGCTA TGTACACAAG AGCAAGGTGA CTGTCTCCAG TGACAGATTC
    AGATTCATCA TCAGCAATGG ACTGCGGACC GAGCACGGGG TGTTTGAGAT CACACTGGAG
    ACTGTGGACA GAGCCCTGCC TGTGGTAACC AGGAACAAGG GGTTGAGACT GGCCCAAGGG
    GCCGTGGGCC TGCTTTCCCC TGACCTCCTT CAGCTGACCG ACCCTGATAC ACCTGTGGAG
    AACCTCACCT TCCTCTTGGT TCAGCTTCCC CAGCATGGCC AGCTCTACCT GTGGGGGACA
    GGGCTACTTC AACACAATTT CACCCAGCAG GATGTGGACA GCAGGAATGT GGCCTATCGG
    CACTCAGGAG GGGACTCCCA GACTGACTGC TTTACTTTCA TGGCCACAGA CGGGACAAAC
    CAAGGCTTTA TTGTGAATGG GAGAGTATGG GAAGAACCTG TTTTATTCAC CATTCAGGTA
    GACCAATTGG ACAAAACAGC TCCTCGTATC ACACTCTTGC ATTCCCCTTC TCAAGTGGGG
    CTCCTGAAAA ATGGCTGCTA CGGGATTTAC ATCACTTCCC GCGTGTTGAA GGCATCAGAC
    CCTGACACTG AGGACGATCA GATCATCTTT AAAATTCTAC AAGGCCCAAA ACATGGACAT
    CTGGAGAACA CAACAACAGG TGAATTTATC CATGAGAAAT TTAGCCAAAA GGACTTAAAC
    AGTAAGACTA TTCTTTACAT CATAAACCCA TCTTTGGAAG TAAATTCAGA TACCGTGGAA
    TTTCAAATCA TGGACCCCAC AGGGAACTCG GCCACTCCTC AAATTTTGGA ACTGAAGTGG
    TCTCATATTG AATGGTCACA GACCGAATAT GAAGTCTGTG AGAATGTGGG TGTGTTGCCC
    TTGGAAATTA TCAGAAGGGG ATATTCCATG GACTCGGCCT TTGTGGGTAT AAAGGTCAAC
    CAAGTGTCAG CTGCAGATGG AAAAGATTTC ACCGTGATTC CATCTAAACT GATTCAGTTT
    GACCCAGGAA TGTCAACTAA GATGTGGAAT ATAGCAATTA CCTATGACGG ATTAGAGGAA
    GATGATGAGG TCTTTGAAGT AATTCTGAAC TCCCCTGTGA ATGCAGTTCT TGGCACAAAG
    ACAAAAGCTG CAGTGAAAAT TTTGGACTCA AAAGGAGGAC AATGCCATCC TTCATATTCC
    TCCAACCAAA GCAAGCACAG CACATGGGAG AAGGGCATTT GGCATCTGCT GCCCCCAGGG
    TCTTCCTCAT CCACCACTTC TGGTTCCTTT CATCTGGAAA GAAGACCTCT TCCATCTTCC
    ATGCAGCTAG CAGTCACCAG GGGAGACACC CTGCGGGGCT TTGATTCTAC AGATCTTTCT
    CAAAGGAAGC TTAGGACCCG TGGGAATGGC AAAACAGTTC GTCCATCCTC TGTTTATAGA
    AATGGAACAG ACATCATCTA TAATTATCAT GGGATAGTTT CCTTGAAACT AGAGGATGAC
    AGCTTCCCAA CTCACAAAAG GAAGGCCAAA GTATCCATCA TTAGTCAGCC ACAAAAGACA
    ATCAAAGTGG CAGAACTGCC TCAAGCAGAT AAGGTGGAAT CCACAACTGA CTCACACTTC
    CCCAGACAGG ACCAGTTGCC CTCATTTCCA AAGAACTGCA CTCTGGAATT AAAGGGACTC
    TTCCATTTTG AAGAAGGCAT CCAGAAGCTG TATCAGTGCA ATGGGATTGC CTGGAAAGCC
    TGGAGTCCCC AAACCAAGGA TGTGGAAGAA AAATCCTGTC CAGCCGGGTG GCACCAGCAC
    TCAGGCTACT GTCACATCTT GATCACAGAG CAGAAAGGCA CCTGGAATGC GGCTGCCCAA
    GCTTGCAGGG AACAATACCT GGGCAACCTT GTAACTGTAT TCTCCAGGCA GCACATGCGG
    TGGCTCTGGG ACATTGGTGA GAGAAAGTCC TTTTGGATAG GTTTGAACGA CCAAGTGCAT
    GCTGGCCACT GGGAGTGGAT CGGTGGTGAA CCTGTTGCCT TCACCAATGG GAGAAGAGGG
    CCCTCTCAAC GCTCCAAGCT TGGAAAGAGC TGTGTTTTGG TTCAAAGACA AGGGAAATGG
    CAAACAAAAG ATTGTAGGAG AGCCAAACCT CATAATTATG TGTGTTCCAG AAAACTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OPc109244
    Clone ID Related Accession (Same CDS sequence) XM_024345572.1
    Accession Version XM_024345572.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6417bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-03-19
    Organism Pan troglodytes(chimpanzee)
    Product FRAS1-related extracellular matrix protein 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036888.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    ATGAACTCTC TGAGTTGGGG GGCTGCGAAT GCCGTGCTGC TGCTGCTCCT CCTGGCCTGG 
    GCCAGCCCCA CCTTCATCAG CATCAACCGT GGGGTGAGGG TGATGAAGGG CCACTCTGCC
    TTCCTGTCAG GAGATGACCT GAAGTTTGCC ATCCCTAAAG AGAAAGATGC CTGCAAAGTG
    GAAGTTGTGA TGAATGAGCC AATAACCCAG AGGGTTGGGA AACTCACTCC ACAGGTCTTT
    GACTGCCATT TCCTTCCCAA TGAAGTCAAG TATGTTCACA ATGGTTGTCC AATTCTTGAT
    GAAGACACAG TGAAGCTTAG ACTTTACAGA TTTACTGAAA GAGATACCTT CATAGAAACC
    TTTATCCTGT GGGTCTATCT CCTGGAACCA GACTGTAACA TCATCCATAT GAGTAACAAT
    GTGCTGGAGG TGCCTGAATT CAATGGCCTG TCCCAAGCGA TTGATAAAAA TCTGCTCAGA
    TTCGATTATG ATAGGATGGC TAGCCTGGAA TGTACGGTCA GCCTGGACAC TGCGAGAACT
    CGGCTGCCAG CCCATGGCCA GATGGTTCTC GGGGAGCCTC GACCAGAAGA ACCTCGTGGA
    GATCAGCCAC ACAGTTTTTT CCCAGAGTCC CGTATGATGT CTTTTAATGC CTTTCTGGAA
    CTGAGAGCAA AACTGAAGTG TCCAGGTGGA AGCTGTACCC CAGGATTAAA GAAAATAGGA
    AGCCTCAAAG TGAGCTGTGA GGAGTTCCTG CTGATGGGCC TTCGTTATCA GCATCTGGAT
    CCCCCTTCAC CCAACATTGA TTATATCTCC ATCCAACTGG ACCTCACAGA TACCAGGAGT
    AAAATTGTGT ACAAGTCAGA GAGTGCGTGG CTGCCTGTCT ATATCAGAGC TGGAATTCCG
    AATCAGATTC CAAAGGCTGC ATTCATGGCC ATGTTTATTC TGGAAGTGGA TCAGTTCATC
    CTGACCTCCT TGACTACATC AGTTCTGGAC TGTGAAGAAG ATGAGACCCC TAAACCCTTG
    CTGGTGTTCA ACATTACTAA AGCCCCGCTC CAGGGCTATG TGACTCACCT GTTGGATCAC
    ACCAGACCAA TCTCCTCATT CACCTGGAAA GATCTCAGTG ACATGCAGAT CGCCTATCAG
    CCACCAAACA GCAGCCATTC TGAGAGGAGA CATGATGAGG TAGAATTGGA GGTATACGAC
    TTCTTCTTTG AAAGGAGTGC ACCTATGACA GTCCACATCT CCATCAGAAC AGCAGATACA
    AATGCCCCCC GTGTATCCTG GAATACAGGT CTGAATCTCC TTGAGGGGCA GTCTCGAGCC
    ATCACTTGGG AACAGTTTCA GGTTGTCGAC AATGACGACA TCGGTGCTGT CCGGCTAGTC
    ACCGTTGGTG GCCTGCAGCA TGGATGGCTG ACTTTAAGAG GGGGGAAAGG ATTTCTCTTC
    ACCGTGGCTG ACCTCCAGGC TGGAGTTGTT CGCTATCATC ATGATGACAG CGACTCCACC
    AAAGACTTCG TGGTCTTCCG GATATTTGAT GGCCATCACA GCATCCGTCA CAAATTCCCC
    ATCAACGTCT TGCCCAAAGA TGATAGTCCC CCGTTCCTCA TAACCAATGT TGTCATTGAA
    CTGGAGGAGG GGCAGACCAT CCTGATCCAG GGATCCATGC TGCGAGCTTC AGATGTGGAT
    GCCAGTGATG ACTACATCTT CTTCAATATC ACAAAGCCTC CACAGGCTGG GGAGATCATG
    AAGAAGCCAG GGCCAGGACT GATAGGCTAT CCTGTCCCTG GCTTCCTTCA GAGGGATTTG
    TTTAATGGAA TCATTTATTA TCGTCATTTT GGTGGAGAAA TCTTTGAAGA TTCTTTTCAA
    TTTGTCCTGT GGGACAGCCA TGAACCTCCA AATCTTTCAG TGCCACAGGT GGCAACAATC
    CATGTAACTC CAGTGGATGA CCAGCTTCCA AAAGAGGCTC CTGGAGTTTC TCGGCATTTG
    GTTGTCAAGG AAACTGAGGT GGCCTATATA ACTAAGAAAC AGCTACATTT TATAGATTCA
    GAATCATATG ACAGGGAGCT GGTCTACACG ATAACTACTC CTCCATTTTT CTCCTTCAGC
    CACAGACACT TGGATGCTGG GAAATTATTT ATGGTGGACA GCATACCAAA AGTAGTTAAG
    AATCCTACGG CCCTGGAGCT GAGGTCATTC ACTCAGCATG CTGTGAACTA TATGAAAGTG
    GCCTACATGC CCCCCATGCA AGACATTGGT CCCCATTGCA GAGATGTCCA GTTCACATTT
    TCTGTCAGTA ACCAACATGG CGGCACTTTG CATGGGATCT GCTTTAACAT TACAATCCTC
    CCAGTGGACA ATCAAGTTCC TGAGGCGTTC ACCAACCCTC TGAAAGTGAC TGAGGGAGGT
    CAAAGCATCA TCAGCACAGA GCACATTCTG ATTTCTGATG CAGATACCAA GCTGGACAAT
    ATTGACCTCT CCCTGCGGGA ATTGCCTCTG CACGGAAGGG TGGAGCTGAA TGGATTTCCT
    CTAAATTCAG GGGGCACATT TTCTTGGGGC GATCTCCATA CCTTAAAAGT TAGGTATCAA
    CATGATGGAA CTGAAGTTCT TCAGGATGAC CTACTCTTGG AGGTCACCGA TGGCACAAAT
    TCAGCAGAAT TTGTTCTACA TGTTGAGGTA TTCCCTGTCA ACGATGAGCC ACCAGTCTTA
    AAGGCTGACC TCATGCCTGT CATGAATTGC TCAGAGGGAG GAGAGGTGGT CATCACCTCT
    GAATACATTT TTGCTACTGA TGTGGACAGC GATAACTTGA AGTTGATGTT TGTGATTGCT
    CGCGAACCTC AGCATGGGGT GGTGAGGAGA GCTGGAGTCA CAGTGGATCA GTTCTCTCAG
    AGAGATGTTA TCTCAGAGGC CGTGACATAC AAACACACAG GTGGCGAGAT TGGCATGATG
    CCCTGTTTTG ACACCATTAC ATTGGTGGTT TCGGATGGAG AGGCTGGCCC TTTTGTGAAT
    GGCTGTTGCT ACAATGGACC CAATCCATCT GTTCCGCTTC ATGCGTCCTT TCCAGTGTAT
    GATCTCAACA TCACGGTATA CCCAGTAGAC AACCAGCCAC CTTCCATTGC AATAGGTCCC
    ATGTTTGTTG TAGATGAGGG CTGCTCAACA GCCCTTACTG TTAACCATTT GTCCGCCACT
    GACCCTGACA CTGCAGCAGA TGACTTGGAA TTTGTTTTGG TTTCTCCTCC TCAGTTTGGC
    TACCTCGAAA ATATACTCCC TTCTGTGGGT TTTGAAAAAA GCAATATTGG CATAAGTATA
    GATTCATTTC AGTGGAAAGA CATGAACGCT TTTCACATTA ACTATGTGCA GTCCAGGCAT
    CTGAGGATAG AACCAACTGC CGACCAGTTC ACGGTGTACG TCACAGATGG GAAGCAACAC
    TCCTTGGAGA TACCATTTTC TATTATAATC AACCCCACAA ATGATGAAGC TCCTGACTTT
    GTAGTGCAGA ATATTACTGT GTGCGAGGGT CAGATGAAAG AGCTGGACTC TTCCATCATC
    AGCGCTGTGG ACCTGGACAT TCCCCAGGAT GCCCTGCTGT TCAGCATCAC TCAAAAGCCA
    CGCCATGGCC TCCTCATCGA TAGGGGGTTT AGCAAAGACT TCTCTGAGAA TAAGCAGCCA
    GCCAACCCTC ACCAGAAACA TGTACCTGTT CACAGTTTTT CCATGGAACT CCTCAAGACT
    GGAATGAGGT TGACGTACAT GCATGATGAC TCAGAGAGCC TTGCTGATGA TTTTACAATC
    CGTTTGTCAG ATGGGAAACA TAAGATACTT AAAACCATTT CAATAGAGGT CATCCCAGTT
    AATGATGAAA AACCAATGCT GAGCAAGAAG GCTGAAATTG CAATGAATAT GGGTGAAACT
    CGTATTATTT CCAGTGCTAT TCTTTCAGCC ATAGATGAAG ACTCACCCAG GGAGAAGATT
    TACTATGTAT TTGAAAGGCT TCCCCAAAAT GGGCAACTTC AGCTTAAGAT AGGGAGGGAC
    TGGGTTCCTC TCTCCCCTGG CATGAAATGC ACTCAGGAGG AAGTGGATCT GAACTTGCTG
    AGATACACAC ACACCGGGGC AATGGATTCC CAGAATCAAG ATAGCTTCAC CTTCTACCTT
    TGGGATGGCA ACAACAGGTC CCCTGCTCTT GACTGTCAAA TCACCATCAA GGACATGGAA
    AAAGGTGATA TTGTCATCCT TACAAAACCA CTCGTGGTGT CTAAAGGTGA TAGAGGTTTC
    TTAACAACCA CCACTCTCCT GGCTGTGGAC GGAACAGACA AGCCTGAGGA ACTGCTCTAT
    GTCATCACCT CCCCTCCACG ATATGGCCAG ATCGAATATG TTCACTATCC TGGAGTTCCC
    ATTACAAACT TCAGCCAAAT GGATATAGCG GGGCAGACAG TTTGCTATGT ACACAAGAGC
    AAGGTGACTG TCTCCAGTGA CAGATTCAGA TTCATCATCA GCAATGGACT GCGGACCGAG
    CACGGGGTGT TTGAGATCAC ACTGGAGACT GTGGACAGAG CCCTGCCTGT GGTAACCAGG
    AACAAGGGGT TGAGACTGGC CCAAGGGGCC GTGGGCCTGC TTTCCCCTGA CCTCCTTCAG
    CTGACCGACC CTGATACACC TGTGGAGAAC CTCACCTTCC TCTTGGTTCA GCTTCCCCAG
    CATGGCCAGC TCTACCTGTG GGGGACAGGG CTACTTCAAC ACAATTTCAC CCAGCAGGAT
    GTGGACAGCA GGAATGTGGC CTATCGGCAC TCAGGAGGGG ACTCCCAGAC TGACTGCTTT
    ACTTTCATGG CCACAGACGG GACAAACCAA GGCTTTATTG TGAATGGGAG AGTATGGGAA
    GAACCTGTTT TATTCACCAT TCAGGTAGAC CAATTGGACA AAACAGCTCC TCGTATCACA
    CTCTTGCATT CCCCTTCTCA AGTGGGGCTC CTGAAAAATG GCTGCTACGG GATTTACATC
    ACTTCCCGCG TGTTGAAGGC ATCAGACCCT GACACTGAGG ACGATCAGAT CATCTTTAAA
    ATTCTACAAG GCCCAAAACA TGGACATCTG GAGAACACAA CAACAGGTGA ATTTATCCAT
    GAGAAATTTA GCCAAAAGGA CTTAAACAGT AAGACTATTC TTTACATCAT AAACCCATCT
    TTGGAAGTAA ATTCAGATAC CGTGGAATTT CAAATCATGG ACCCCACAGG GAACTCGGCC
    ACTCCTCAAA TTTTGGAACT GAAGTGGTCT CATATTGAAT GGTCACAGAC CGAATATGAA
    GTCTGTGAGA ATGTGGGTGT GTTGCCCTTG GAAATTATCA GAAGGGGATA TTCCATGGAC
    TCGGCCTTTG TGGGTATAAA GGTCAACCAA GTGTCAGCTG CAGATGGAAA AGATTTCACC
    GTGATTCCAT CTAAACTGAT TCAGTTTGAC CCAGGACAAT GCCATCCTTC ATATTCCTCC
    AACCAAAGCA AGCACAGCAC ATGGGAGAAG GGCATTTGGC ATCTGCTGCC CCCAGGGTCT
    TCCTCATCCA CCACTTCTGG TTCCTTTCAT CTGGAAAGAA GACCTCTTCC ATCTTCCATG
    CAGCTAGCAG TCACCAGGGG AGACACCCTG CGGGGCTTTG ATTCTACAGA TCTTTCTCAA
    AGGAAGCTTA GGACCCGTGG GAATGGCAAA ACAGTTCGTC CATCCTCTGT TTATAGAAAT
    GGAACAGACA TCATCTATAA TTATCATGGG ATAGTTTCCT TGAAACTAGA GGATGACAGC
    TTCCCAACTC ACAAAAGGAA GGCCAAAGTA TCCATCATTA GTCAGCCACA AAAGACAATC
    AAAGTGGCAG AACTGCCTCA AGCAGATAAG GTGGAATCCA CAACTGACTC ACACTTCCCC
    AGACAGGACC AGTTGCCCTC ATTTCCAAAG AACTGCACTC TGGAATTAAA GGGACTCTTC
    CATTTTGAAG AAGGCATCCA GAAGCTGTAT CAGTGCAATG GGATTGCCTG GAAAGCCTGG
    AGTCCCCAAA CCAAGGATGT GGAAGAAAAA TCCTGTCCAG CCGGGTGGCA CCAGCACTCA
    GGCTACTGTC ACATCTTGAT CACAGAGCAG AAAGGCACCT GGAATGCGGC TGCCCAAGCT
    TGCAGGGAAC AATACCTGGG CAACCTTGTA ACTGTATTCT CCAGGCAGCA CATGCGGTGG
    CTCTGGGACA TTGGTGAGAG AAAGTCCTTT TGGATAGGTT TGAACGACCA AGTGCATGCT
    GGCCACTGGG AGTGGATCGG TGGTGAACCT GTTGCCTTCA CCAATGGGAG AAGAGGGCCC
    TCTCAACGCT CCAAGCTTGG AAAGAGCTGT GTTTTGGTTC AAAGACAAGG GAAATGGCAA
    ACAAAAGATT GTAGGAGAGC CAAACCTCAT AATTATGTGT GTTCCAGAAA ACTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024201340.1
    CDS1379..7795
    Translation

    Target ORF information:

    RefSeq Version XM_024345572.1
    Organism Pan troglodytes(chimpanzee)
    Definition Pan troglodytes FRAS1 related extracellular matrix 1 (FREM1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024345572.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    ATGAACTCTC TGAGTTGGGG GGCTGCGAAT GCCGTGCTGC TGCTGCTCCT CCTGGCCTGG 
    GCCAGCCCCA CCTTCATCAG CATCAACCGT GGGGTGAGGG TGATGAAGGG CCACTCTGCC
    TTCCTGTCAG GAGATGACCT GAAGTTTGCC ATCCCTAAAG AGAAAGATGC CTGCAAAGTG
    GAAGTTGTGA TGAATGAGCC AATAACCCAG AGGGTTGGGA AACTCACTCC ACAGGTCTTT
    GACTGCCATT TCCTTCCCAA TGAAGTCAAG TATGTTCACA ATGGTTGTCC AATTCTTGAT
    GAAGACACAG TGAAGCTTAG ACTTTACAGA TTTACTGAAA GAGATACCTT CATAGAAACC
    TTTATCCTGT GGGTCTATCT CCTGGAACCA GACTGTAACA TCATCCATAT GAGTAACAAT
    GTGCTGGAGG TGCCTGAATT CAATGGCCTG TCCCAAGCGA TTGATAAAAA TCTGCTCAGA
    TTCGATTATG ATAGGATGGC TAGCCTGGAA TGTACGGTCA GCCTGGACAC TGCGAGAACT
    CGGCTGCCAG CCCATGGCCA GATGGTTCTC GGGGAGCCTC GACCAGAAGA ACCTCGTGGA
    GATCAGCCAC ACAGTTTTTT CCCAGAGTCC CGTATGATGT CTTTTAATGC CTTTCTGGAA
    CTGAGAGCAA AACTGAAGTG TCCAGGTGGA AGCTGTACCC CAGGATTAAA GAAAATAGGA
    AGCCTCAAAG TGAGCTGTGA GGAGTTCCTG CTGATGGGCC TTCGTTATCA GCATCTGGAT
    CCCCCTTCAC CCAACATTGA TTATATCTCC ATCCAACTGG ACCTCACAGA TACCAGGAGT
    AAAATTGTGT ACAAGTCAGA GAGTGCGTGG CTGCCTGTCT ATATCAGAGC TGGAATTCCG
    AATCAGATTC CAAAGGCTGC ATTCATGGCC ATGTTTATTC TGGAAGTGGA TCAGTTCATC
    CTGACCTCCT TGACTACATC AGTTCTGGAC TGTGAAGAAG ATGAGACCCC TAAACCCTTG
    CTGGTGTTCA ACATTACTAA AGCCCCGCTC CAGGGCTATG TGACTCACCT GTTGGATCAC
    ACCAGACCAA TCTCCTCATT CACCTGGAAA GATCTCAGTG ACATGCAGAT CGCCTATCAG
    CCACCAAACA GCAGCCATTC TGAGAGGAGA CATGATGAGG TAGAATTGGA GGTATACGAC
    TTCTTCTTTG AAAGGAGTGC ACCTATGACA GTCCACATCT CCATCAGAAC AGCAGATACA
    AATGCCCCCC GTGTATCCTG GAATACAGGT CTGAATCTCC TTGAGGGGCA GTCTCGAGCC
    ATCACTTGGG AACAGTTTCA GGTTGTCGAC AATGACGACA TCGGTGCTGT CCGGCTAGTC
    ACCGTTGGTG GCCTGCAGCA TGGATGGCTG ACTTTAAGAG GGGGGAAAGG ATTTCTCTTC
    ACCGTGGCTG ACCTCCAGGC TGGAGTTGTT CGCTATCATC ATGATGACAG CGACTCCACC
    AAAGACTTCG TGGTCTTCCG GATATTTGAT GGCCATCACA GCATCCGTCA CAAATTCCCC
    ATCAACGTCT TGCCCAAAGA TGATAGTCCC CCGTTCCTCA TAACCAATGT TGTCATTGAA
    CTGGAGGAGG GGCAGACCAT CCTGATCCAG GGATCCATGC TGCGAGCTTC AGATGTGGAT
    GCCAGTGATG ACTACATCTT CTTCAATATC ACAAAGCCTC CACAGGCTGG GGAGATCATG
    AAGAAGCCAG GGCCAGGACT GATAGGCTAT CCTGTCCCTG GCTTCCTTCA GAGGGATTTG
    TTTAATGGAA TCATTTATTA TCGTCATTTT GGTGGAGAAA TCTTTGAAGA TTCTTTTCAA
    TTTGTCCTGT GGGACAGCCA TGAACCTCCA AATCTTTCAG TGCCACAGGT GGCAACAATC
    CATGTAACTC CAGTGGATGA CCAGCTTCCA AAAGAGGCTC CTGGAGTTTC TCGGCATTTG
    GTTGTCAAGG AAACTGAGGT GGCCTATATA ACTAAGAAAC AGCTACATTT TATAGATTCA
    GAATCATATG ACAGGGAGCT GGTCTACACG ATAACTACTC CTCCATTTTT CTCCTTCAGC
    CACAGACACT TGGATGCTGG GAAATTATTT ATGGTGGACA GCATACCAAA AGTAGTTAAG
    AATCCTACGG CCCTGGAGCT GAGGTCATTC ACTCAGCATG CTGTGAACTA TATGAAAGTG
    GCCTACATGC CCCCCATGCA AGACATTGGT CCCCATTGCA GAGATGTCCA GTTCACATTT
    TCTGTCAGTA ACCAACATGG CGGCACTTTG CATGGGATCT GCTTTAACAT TACAATCCTC
    CCAGTGGACA ATCAAGTTCC TGAGGCGTTC ACCAACCCTC TGAAAGTGAC TGAGGGAGGT
    CAAAGCATCA TCAGCACAGA GCACATTCTG ATTTCTGATG CAGATACCAA GCTGGACAAT
    ATTGACCTCT CCCTGCGGGA ATTGCCTCTG CACGGAAGGG TGGAGCTGAA TGGATTTCCT
    CTAAATTCAG GGGGCACATT TTCTTGGGGC GATCTCCATA CCTTAAAAGT TAGGTATCAA
    CATGATGGAA CTGAAGTTCT TCAGGATGAC CTACTCTTGG AGGTCACCGA TGGCACAAAT
    TCAGCAGAAT TTGTTCTACA TGTTGAGGTA TTCCCTGTCA ACGATGAGCC ACCAGTCTTA
    AAGGCTGACC TCATGCCTGT CATGAATTGC TCAGAGGGAG GAGAGGTGGT CATCACCTCT
    GAATACATTT TTGCTACTGA TGTGGACAGC GATAACTTGA AGTTGATGTT TGTGATTGCT
    CGCGAACCTC AGCATGGGGT GGTGAGGAGA GCTGGAGTCA CAGTGGATCA GTTCTCTCAG
    AGAGATGTTA TCTCAGAGGC CGTGACATAC AAACACACAG GTGGCGAGAT TGGCATGATG
    CCCTGTTTTG ACACCATTAC ATTGGTGGTT TCGGATGGAG AGGCTGGCCC TTTTGTGAAT
    GGCTGTTGCT ACAATGGACC CAATCCATCT GTTCCGCTTC ATGCGTCCTT TCCAGTGTAT
    GATCTCAACA TCACGGTATA CCCAGTAGAC AACCAGCCAC CTTCCATTGC AATAGGTCCC
    ATGTTTGTTG TAGATGAGGG CTGCTCAACA GCCCTTACTG TTAACCATTT GTCCGCCACT
    GACCCTGACA CTGCAGCAGA TGACTTGGAA TTTGTTTTGG TTTCTCCTCC TCAGTTTGGC
    TACCTCGAAA ATATACTCCC TTCTGTGGGT TTTGAAAAAA GCAATATTGG CATAAGTATA
    GATTCATTTC AGTGGAAAGA CATGAACGCT TTTCACATTA ACTATGTGCA GTCCAGGCAT
    CTGAGGATAG AACCAACTGC CGACCAGTTC ACGGTGTACG TCACAGATGG GAAGCAACAC
    TCCTTGGAGA TACCATTTTC TATTATAATC AACCCCACAA ATGATGAAGC TCCTGACTTT
    GTAGTGCAGA ATATTACTGT GTGCGAGGGT CAGATGAAAG AGCTGGACTC TTCCATCATC
    AGCGCTGTGG ACCTGGACAT TCCCCAGGAT GCCCTGCTGT TCAGCATCAC TCAAAAGCCA
    CGCCATGGCC TCCTCATCGA TAGGGGGTTT AGCAAAGACT TCTCTGAGAA TAAGCAGCCA
    GCCAACCCTC ACCAGAAACA TGTACCTGTT CACAGTTTTT CCATGGAACT CCTCAAGACT
    GGAATGAGGT TGACGTACAT GCATGATGAC TCAGAGAGCC TTGCTGATGA TTTTACAATC
    CGTTTGTCAG ATGGGAAACA TAAGATACTT AAAACCATTT CAATAGAGGT CATCCCAGTT
    AATGATGAAA AACCAATGCT GAGCAAGAAG GCTGAAATTG CAATGAATAT GGGTGAAACT
    CGTATTATTT CCAGTGCTAT TCTTTCAGCC ATAGATGAAG ACTCACCCAG GGAGAAGATT
    TACTATGTAT TTGAAAGGCT TCCCCAAAAT GGGCAACTTC AGCTTAAGAT AGGGAGGGAC
    TGGGTTCCTC TCTCCCCTGG CATGAAATGC ACTCAGGAGG AAGTGGATCT GAACTTGCTG
    AGATACACAC ACACCGGGGC AATGGATTCC CAGAATCAAG ATAGCTTCAC CTTCTACCTT
    TGGGATGGCA ACAACAGGTC CCCTGCTCTT GACTGTCAAA TCACCATCAA GGACATGGAA
    AAAGGTGATA TTGTCATCCT TACAAAACCA CTCGTGGTGT CTAAAGGTGA TAGAGGTTTC
    TTAACAACCA CCACTCTCCT GGCTGTGGAC GGAACAGACA AGCCTGAGGA ACTGCTCTAT
    GTCATCACCT CCCCTCCACG ATATGGCCAG ATCGAATATG TTCACTATCC TGGAGTTCCC
    ATTACAAACT TCAGCCAAAT GGATATAGCG GGGCAGACAG TTTGCTATGT ACACAAGAGC
    AAGGTGACTG TCTCCAGTGA CAGATTCAGA TTCATCATCA GCAATGGACT GCGGACCGAG
    CACGGGGTGT TTGAGATCAC ACTGGAGACT GTGGACAGAG CCCTGCCTGT GGTAACCAGG
    AACAAGGGGT TGAGACTGGC CCAAGGGGCC GTGGGCCTGC TTTCCCCTGA CCTCCTTCAG
    CTGACCGACC CTGATACACC TGTGGAGAAC CTCACCTTCC TCTTGGTTCA GCTTCCCCAG
    CATGGCCAGC TCTACCTGTG GGGGACAGGG CTACTTCAAC ACAATTTCAC CCAGCAGGAT
    GTGGACAGCA GGAATGTGGC CTATCGGCAC TCAGGAGGGG ACTCCCAGAC TGACTGCTTT
    ACTTTCATGG CCACAGACGG GACAAACCAA GGCTTTATTG TGAATGGGAG AGTATGGGAA
    GAACCTGTTT TATTCACCAT TCAGGTAGAC CAATTGGACA AAACAGCTCC TCGTATCACA
    CTCTTGCATT CCCCTTCTCA AGTGGGGCTC CTGAAAAATG GCTGCTACGG GATTTACATC
    ACTTCCCGCG TGTTGAAGGC ATCAGACCCT GACACTGAGG ACGATCAGAT CATCTTTAAA
    ATTCTACAAG GCCCAAAACA TGGACATCTG GAGAACACAA CAACAGGTGA ATTTATCCAT
    GAGAAATTTA GCCAAAAGGA CTTAAACAGT AAGACTATTC TTTACATCAT AAACCCATCT
    TTGGAAGTAA ATTCAGATAC CGTGGAATTT CAAATCATGG ACCCCACAGG GAACTCGGCC
    ACTCCTCAAA TTTTGGAACT GAAGTGGTCT CATATTGAAT GGTCACAGAC CGAATATGAA
    GTCTGTGAGA ATGTGGGTGT GTTGCCCTTG GAAATTATCA GAAGGGGATA TTCCATGGAC
    TCGGCCTTTG TGGGTATAAA GGTCAACCAA GTGTCAGCTG CAGATGGAAA AGATTTCACC
    GTGATTCCAT CTAAACTGAT TCAGTTTGAC CCAGGACAAT GCCATCCTTC ATATTCCTCC
    AACCAAAGCA AGCACAGCAC ATGGGAGAAG GGCATTTGGC ATCTGCTGCC CCCAGGGTCT
    TCCTCATCCA CCACTTCTGG TTCCTTTCAT CTGGAAAGAA GACCTCTTCC ATCTTCCATG
    CAGCTAGCAG TCACCAGGGG AGACACCCTG CGGGGCTTTG ATTCTACAGA TCTTTCTCAA
    AGGAAGCTTA GGACCCGTGG GAATGGCAAA ACAGTTCGTC CATCCTCTGT TTATAGAAAT
    GGAACAGACA TCATCTATAA TTATCATGGG ATAGTTTCCT TGAAACTAGA GGATGACAGC
    TTCCCAACTC ACAAAAGGAA GGCCAAAGTA TCCATCATTA GTCAGCCACA AAAGACAATC
    AAAGTGGCAG AACTGCCTCA AGCAGATAAG GTGGAATCCA CAACTGACTC ACACTTCCCC
    AGACAGGACC AGTTGCCCTC ATTTCCAAAG AACTGCACTC TGGAATTAAA GGGACTCTTC
    CATTTTGAAG AAGGCATCCA GAAGCTGTAT CAGTGCAATG GGATTGCCTG GAAAGCCTGG
    AGTCCCCAAA CCAAGGATGT GGAAGAAAAA TCCTGTCCAG CCGGGTGGCA CCAGCACTCA
    GGCTACTGTC ACATCTTGAT CACAGAGCAG AAAGGCACCT GGAATGCGGC TGCCCAAGCT
    TGCAGGGAAC AATACCTGGG CAACCTTGTA ACTGTATTCT CCAGGCAGCA CATGCGGTGG
    CTCTGGGACA TTGGTGAGAG AAAGTCCTTT TGGATAGGTT TGAACGACCA AGTGCATGCT
    GGCCACTGGG AGTGGATCGG TGGTGAACCT GTTGCCTTCA CCAATGGGAG AAGAGGGCCC
    TCTCAACGCT CCAAGCTTGG AAAGAGCTGT GTTTTGGTTC AAAGACAAGG GAAATGGCAA
    ACAAAAGATT GTAGGAGAGC CAAACCTCAT AATTATGTGT GTTCCAGAAA ACTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OPc88756
    Clone ID Related Accession (Same CDS sequence) XM_009456318.2 , XM_016960627.1 , XM_016960625.1 , XM_016960626.1
    Accession Version XM_009456318.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6540bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-01
    Organism Pan troglodytes(chimpanzee)
    Product FRAS1-related extracellular matrix protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006476.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 2, 2016 this sequence version replaced XM_009456318.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGAACTCTC TGAGTTGGGG GGCTGCGAAT GCCGTGCTGC TGCTGCTCCT CCTGGCCTGG 
    GCCAGCCCCA CCTTCATCAG CATCAACCGT GGGGTGAGGG TGATGAAGGG CCACTCTGCC
    TTCCTGTCAG GAGATGACCT GAAGTTTGCC ATCCCTAAAG AGAAAGATGC CTGCAAAGTG
    GAAGTTGTGA TGAATGAGCC AATAACCCAG AGGGTTGGGA AACTCACTCC ACAGGTCTTT
    GACTGCCATT TCCTTCCCAA TGAAGTCAAG TATGTTCACA ATGGTTGTCC AATTCTTGAT
    GAAGACACAG TGAAGCTTAG ACTTTACAGA TTTACTGAAA GAGATACCTT CATAGAAACC
    TTTATCCTGT GGGTCTATCT CCTGGAACCA GACTGTAACA TCATCCATAT GAGTAACAAT
    GTGCTGGAGG TGCCTGAATT CAATGGCCTG TCCCAAGCGA TTGATAAAAA TCTGCTCAGA
    TTCGATTATG ATAGGATGGC TAGCCTGGAA TGTACGGTCA GCCTGGACAC TGCGAGAACT
    CGGCTGCCAG CCCATGGCCA GATGGTTCTC GGGGAGCCTC GACCAGAAGA ACCTCGTGGA
    GATCAGCCAC ACAGTTTTTT CCCAGAGTCC CAACTGAGAG CAAAACTGAA GTGTCCAGGT
    GGAAGCTGTA CCCCAGGATT AAAGAAAATA GGAAGCCTCA AAGTGAGCTG TGAGGAGTTC
    CTGCTGATGG GCCTTCGTTA TCAGCATCTG GATCCCCCTT CACCCAACAT TGATTATATC
    TCCATCCAAC TGGACCTCAC AGATACCAGG AGTAAAATTG TGTACAAGTC AGAGAGTGCG
    TGGCTGCCTG TCTATATCAG AGCTGGAATT CCGAATCAGA TTCCAAAGGC TGCATTCATG
    GCCATGTTTA TTCTGGAAGT GGATCAGTTC ATCCTGACCT CCTTGACTAC ATCAGTTCTG
    GACTGTGAAG AAGATGAGAC CCCTAAACCC TTGCTGGTGT TCAACATTAC TAAAGCCCCG
    CTCCAGGGCT ATGTGACTCA CCTGTTGGAT CACACCAGAC CAATCTCCTC ATTCACCTGG
    AAAGATCTCA GTGACATGCA GATCGCCTAT CAGCCACCAA ACAGCAGCCA TTCTGAGAGG
    AGACATGATG AGGTAGAATT GGAGGTATAC GACTTCTTCT TTGAAAGGAG TGCACCTATG
    ACAGTCCACA TCTCCATCAG AACAGCAGAT ACAAATGCCC CCCGTGTATC CTGGAATACA
    GGTCTGAATC TCCTTGAGGG GCAGTCTCGA GCCATCACTT GGGAACAGTT TCAGGTTGTC
    GACAATGACG ACATCGGTGC TGTCCGGCTA GTCACCGTTG GTGGCCTGCA GCATGGATGG
    CTGACTTTAA GAGGGGGGAA AGGATTTCTC TTCACCGTGG CTGACCTCCA GGCTGGAGTT
    GTTCGCTATC ATCATGATGA CAGCGACTCC ACCAAAGACT TCGTGGTCTT CCGGATATTT
    GATGGCCATC ACAGCATCCG TCACAAATTC CCCATCAACG TCTTGCCCAA AGATGATAGT
    CCCCCGTTCC TCATAACCAA TGTTGTCATT GAACTGGAGG AGGGGCAGAC CATCCTGATC
    CAGGGATCCA TGCTGCGAGC TTCAGATGTG GATGCCAGTG ATGACTACAT CTTCTTCAAT
    ATCACAAAGC CTCCACAGGC TGGGGAGATC ATGAAGAAGC CAGGGCCAGG ACTGATAGGC
    TATCCTGTCC CTGGCTTCCT TCAGAGGGAT TTGTTTAATG GAATCATTTA TTATCGTCAT
    TTTGGTGGAG AAATCTTTGA AGATTCTTTT CAATTTGTCC TGTGGGACAG CCATGAACCT
    CCAAATCTTT CAGTGCCACA GGTGGCAACA ATCCATGTAA CTCCAGTGGA TGACCAGCTT
    CCAAAAGAGG CTCCTGGAGT TTCTCGGCAT TTGGTTGTCA AGGAAACTGA GGTGGCCTAT
    ATAACTAAGA AACAGCTACA TTTTATAGAT TCAGAATCAT ATGACAGGGA GCTGGTCTAC
    ACGATAACTA CTCCTCCATT TTTCTCCTTC AGCCACAGAC ACTTGGATGC TGGGAAATTA
    TTTATGGTGG ACAGCATACC AAAAGTAGTT AAGAATCCTA CGGCCCTGGA GCTGAGGTCA
    TTCACTCAGC ATGCTGTGAA CTATATGAAA GTGGCCTACA TGCCCCCCAT GCAAGACATT
    GGTCCCCATT GCAGAGATGT CCAGTTCACA TTTTCTGTCA GTAACCAACA TGGCGGCACT
    TTGCATGGGA TCTGCTTTAA CATTACAATC CTCCCAGTGG ACAATCAAGT TCCTGAGGCG
    TTCACCAACC CTCTGAAAGT GACTGAGGGA GGTCAAAGCA TCATCAGCAC AGAGCACATT
    CTGATTTCTG ATGCAGATAC CAAGCTGGAC AATATTGACC TCTCCCTGCG GGAATTGCCT
    CTGCACGGAA GGGTGGAGCT GAATGGATTT CCTCTAAATT CAGGGGGCAC ATTTTCTTGG
    GGCGATCTCC ATACCTTAAA AGTTAGGTAT CAACATGATG GAACTGAAGT TCTTCAGGAT
    GACCTACTCT TGGAGGTCAC CGATGGCACA AATTCAGCAG AATTTGTTCT ACATGTTGAG
    GTATTCCCTG TCAACGATGA GCCACCAGTC TTAAAGGCTG ACCTCATGCC TGTCATGAAT
    TGCTCAGAGG GAGGAGAGGT GGTCATCACC TCTGAATACA TTTTTGCTAC TGATGTGGAC
    AGCGATAACT TGAAGTTGAT GTTTGTGATT GCTCGCGAAC CTCAGCATGG GGTGGTGAGG
    AGAGCTGGAG TCACAGTGGA TCAGTTCTCT CAGAGAGATG TTATCTCAGA GGCCGTGACA
    TACAAACACA CAGGTGGCGA GATTGGCATG ATGCCCTGTT TTGACACCAT TACATTGGTG
    GTTTCGGATG GAGAGGCTGG CCCTTTTGTG AATGGCTGTT GCTACAATGG ACCCAATCCA
    TCTGTTCCGC TTCATGCGTC CTTTCCAGTG TATGATCTCA ACATCACGGT ATACCCAGTA
    GACAACCAGC CACCTTCCGT TGCAATAGGT CCCATGTTTG TTGTAGATGA GGGCTGCTCA
    ACAGCCCTTA CTGTTAACCA TTTGTCCGCC ACTGACCCTG ACACTGCAGC AGATGACTTG
    GAATTTGTTT TGGTTTCTCC TCCTCAGTTT GGCTACCTCG AAAATATACT CCCTTCTGTG
    GGTTTTGAAA AAAGCAATAT TGGCATAAGT ATAGATTCAT TTCAGTGGAA AGACATGAAC
    GCTTTTCACA TTAACTATGT GCAGTCCAGG CATCTGAGGA TAGAACCAAC TGCCGACCAG
    TTCACGGTGT ACGTCACAGA TGGGAAGCAA CACTCCTTGG AGATACCATT TTCTATTATA
    ATCAACCCCA CAAATGATGA AGCTCCTGAC TTTGTAGTGC AGAATATTAC TGTGTGCGAG
    GGTCAGATGA AAGAGCTGGA CTCTTCCATC ATCAGCGCTG TGGACCTGGA CATTCCCCAG
    GATGCCCTGC TGTTCAGCAT CACTCAAAAG CCACGCCATG GCCTCCTCAT CGATAGGGGG
    TTTAGCAAAG ACTTCTCTGA GAATAAGCAG CCAGCCAACC CTCACCAGAA ACATGTACCT
    GTTCACAGTT TTTCCATGGA ACTCCTCAAG ACTGGAATGA GGTTGACGTA CATGCATGAT
    GACTCAGAGA GCCTTGCTGA TGATTTTACA ATCCGTTTGT CAGATGGGAA ACATAAGATA
    CTTAAAACCA TTTCAATAGA GGTCATCCCA GTTAATGATG AAAAACCAAT GCTGAGCAAG
    AAGGCTGAAA TTGCAATGAA TATGGGTGAA ACTCGTATTA TTTCCAGTGC TATTCTTTCA
    GCCATAGATG AAGACTCACC CAGGGAGAAG ATTTACTATG TATTTGAAAG GCTTCCCCAA
    AATGGGCAAC TTCAGCTTAA GATAGGGAGG GACTGGGTTC CTCTCTCCCC TGGCATGAAA
    TGCACTCAGG AGGAAGTGGA TCTGAACTTG CTGAGATACA CACACACCGG GGCAATGGAT
    TCCCAGAATC AAGATAGCTT CACCTTCTAC CTTTGGGATG GCAACAACAG GTCCCCTGCT
    CTTGACTGTC AAATCACCAT CAAGGACATG GAAAAAGGTG ATATTGTCAT CCTTACAAAA
    CCACTCGTGG TGTCTAAAGG TGATAGAGGT TTCTTAACAA CCACCACTCT CCTGGCTGTG
    GACGGAACAG ACAAGCCTGA GGAACTGCTC TATGTCATCA CCTCCCCTCC ACGATATGGC
    CAGATCGAAT ATGTTCACTA TCCTGGAGTT CCCATTACAA ACTTCAGCCA AATGGATATA
    GCGGGGCAGA CAGTTTGCTA TGTACACAAG AGCAAGGTGA CTGTCTCCAG TGACAGATTC
    AGATTCATCA TCAGCAATGG ACTGCGGACC GAGCACGGGG TGTTTGAGAT CACACTGGAG
    ACTGTGGACA GAGCCCTGCC TGTGGTAACC AGGAACAAGG GGTTGAGACT GGCCCAAGGG
    GCCGTGGGCC TGCTTTCCCC TGACCTCCTT CAGCTGACCG ACCCTGATAC ACCTGTGGAG
    AACCTCACCT TCCTCTTGGT TCAGCTTCCC CAGCATGGCC AGCTCTACCT GTGGGGGACA
    GGGCTACTTC AACACAATTT CACCCAGCAG GATGTGGACA GCAGGAATGT GGCCTATCGG
    CACTCAGGAG GGGACTCCCA GACTGACTGC TTTACTTTCA TGGCCACAGA CGGGACAAAC
    CAAGGCTTTA TTGTGAATGG GAGAGTATGG GAAGAACCTG TTTTATTCAC CATTCAGGTA
    GACCAATTGG ACAAAACAGC TCCTCGTATC ACACTCTTGC ATTCCCCTTC TCAAGTGGGG
    CTCCTGAAAA ATGGCTGCTA CGGGATTTAC ATCACTTCCC GCGTGTTGAA GGCATCAGAC
    CCTGACACTG AGGACGATCA GATCATCTTT AAAATTCTAC AAGGCCCAAA ACATGGACAT
    CTGGAGAACA CAACAACAGG TGAATTTATC CATGAGAAAT TTAGCCAAAA GGACTTAAAC
    AGTAAGACTA TTCTTTACAT CATAAACCCA TCTTTGGAAG TAAATTCAGA TACCGTGGAA
    TTTCAAATCA TGGACCCCAC AGGGAACTCG GCCACTCCTC AAATTTTGGA ACTGAAGTGG
    TCTCATATTG AATGGTCACA GACCGAATAT GAAGTCTGTG AGAATGTGGG TGTGTTGCCC
    TTGGAAATTA TCAGAAGGGG ATATTCCATG GACTCGGCCT TTGTGGGTAT AAAGGTCAAC
    CAAGTGTCAG CTGCAGATGG AAAAGATTTC ACCGTGATTC CATCTAAACT GATTCAGTTT
    GACCCAGGAA TGTCAACTAA GATGTGGAAT ATAGCAATTA CCTATGACGG ATTAGAGGAA
    GATGATGAGG TCTTTGAAGT AATTCTGAAC TCCCCTGTGA ATGCAGTTCT TGGCACAAAG
    ACAAAAGCTG CAGTGAAAAT TTTGGACTCA AAAGGAGGAC AATGCCATCC TTCATATTCC
    TCCAACCAAA GCAAGCACAG CACATGGGAG AAGGGCATTT GGCATCTGCT GCCCCCAGGG
    TCTTCCTCAT CCACCACTTC TGGTTCCTTT CATCTGGAAA GAAGACCTCT TCCATCTTCC
    ATGCAGCTAG CAGTCACCAG GGGAGACACC CTGCGGGGCT TTGATTCTAC AGATCTTTCT
    CAAAGGAAGC TTAGGACCCG TGGGAATGGC AAAACAGTTC GTCCATCCTC TGTTTATAGA
    AATGGAACAG ACATCATCTA TAATTATCAT GGGATAGTTT CCTTGAAACT AGAGGATGAC
    AGCTTCCCAA CTCACAAAAG GAAGGCCAAA GTATCCATCA TTAGTCAGCC ACAAAAGACA
    ATCAAAGTGG CAGAACTGCC TCAAGCAGAT AAGGTGGAAT CCACAACTGA CTCACACTTC
    CCCAGACAGG ACCAGTTGCC CTCATTTCCA AAGAACTGCA CTCTGGAATT AAAGGGACTC
    TTCCATTTTG AAGAAGGCAT CCAGAAGCTG TATCAGTGCA ATGGGATTGC CTGGAAAGCC
    TGGAGTCCCC AAACCAAGGA TGTGGAAGAA AAATCCTGTC CAGCCGGGTG GCACCAGCAC
    TCAGGCTACT GTCACATCTT GATCACAGAG CAGAAAGGCA CCTGGAATGC GGCTGCCCAA
    GCTTGCAGGG AACAATACCT GGGCAACCTT GTAACTGTAT TCTCCAGGCA GCACATGCGG
    TGGCTCTGGG ACATTGGTGA GAGAAAGTCC TTTTGGATAG GTTTGAACGA CCAAGTGCAT
    GCTGGCCACT GGGAGTGGAT CGGTGGTGAA CCTGTTGCCT TCACCAATGG GAGAAGAGGG
    CCCTCTCAAC GCTCCAAGCT TGGAAAGAGC TGTGTTTTGG TTCAAAGACA AGGGAAATGG
    CAAACAAAAG ATTGTAGGAG AGCCAAACCT CATAATTATG TGTGTTCCAG AAAACTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009454593.2
    CDS1809..8348
    Translation

    Target ORF information:

    RefSeq Version XM_009456318.2
    Organism Pan troglodytes(chimpanzee)
    Definition Pan troglodytes FRAS1 related extracellular matrix 1 (FREM1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009456318.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGAACTCTC TGAGTTGGGG GGCTGCGAAT GCCGTGCTGC TGCTGCTCCT CCTGGCCTGG 
    GCCAGCCCCA CCTTCATCAG CATCAACCGT GGGGTGAGGG TGATGAAGGG CCACTCTGCC
    TTCCTGTCAG GAGATGACCT GAAGTTTGCC ATCCCTAAAG AGAAAGATGC CTGCAAAGTG
    GAAGTTGTGA TGAATGAGCC AATAACCCAG AGGGTTGGGA AACTCACTCC ACAGGTCTTT
    GACTGCCATT TCCTTCCCAA TGAAGTCAAG TATGTTCACA ATGGTTGTCC AATTCTTGAT
    GAAGACACAG TGAAGCTTAG ACTTTACAGA TTTACTGAAA GAGATACCTT CATAGAAACC
    TTTATCCTGT GGGTCTATCT CCTGGAACCA GACTGTAACA TCATCCATAT GAGTAACAAT
    GTGCTGGAGG TGCCTGAATT CAATGGCCTG TCCCAAGCGA TTGATAAAAA TCTGCTCAGA
    TTCGATTATG ATAGGATGGC TAGCCTGGAA TGTACGGTCA GCCTGGACAC TGCGAGAACT
    CGGCTGCCAG CCCATGGCCA GATGGTTCTC GGGGAGCCTC GACCAGAAGA ACCTCGTGGA
    GATCAGCCAC ACAGTTTTTT CCCAGAGTCC CAACTGAGAG CAAAACTGAA GTGTCCAGGT
    GGAAGCTGTA CCCCAGGATT AAAGAAAATA GGAAGCCTCA AAGTGAGCTG TGAGGAGTTC
    CTGCTGATGG GCCTTCGTTA TCAGCATCTG GATCCCCCTT CACCCAACAT TGATTATATC
    TCCATCCAAC TGGACCTCAC AGATACCAGG AGTAAAATTG TGTACAAGTC AGAGAGTGCG
    TGGCTGCCTG TCTATATCAG AGCTGGAATT CCGAATCAGA TTCCAAAGGC TGCATTCATG
    GCCATGTTTA TTCTGGAAGT GGATCAGTTC ATCCTGACCT CCTTGACTAC ATCAGTTCTG
    GACTGTGAAG AAGATGAGAC CCCTAAACCC TTGCTGGTGT TCAACATTAC TAAAGCCCCG
    CTCCAGGGCT ATGTGACTCA CCTGTTGGAT CACACCAGAC CAATCTCCTC ATTCACCTGG
    AAAGATCTCA GTGACATGCA GATCGCCTAT CAGCCACCAA ACAGCAGCCA TTCTGAGAGG
    AGACATGATG AGGTAGAATT GGAGGTATAC GACTTCTTCT TTGAAAGGAG TGCACCTATG
    ACAGTCCACA TCTCCATCAG AACAGCAGAT ACAAATGCCC CCCGTGTATC CTGGAATACA
    GGTCTGAATC TCCTTGAGGG GCAGTCTCGA GCCATCACTT GGGAACAGTT TCAGGTTGTC
    GACAATGACG ACATCGGTGC TGTCCGGCTA GTCACCGTTG GTGGCCTGCA GCATGGATGG
    CTGACTTTAA GAGGGGGGAA AGGATTTCTC TTCACCGTGG CTGACCTCCA GGCTGGAGTT
    GTTCGCTATC ATCATGATGA CAGCGACTCC ACCAAAGACT TCGTGGTCTT CCGGATATTT
    GATGGCCATC ACAGCATCCG TCACAAATTC CCCATCAACG TCTTGCCCAA AGATGATAGT
    CCCCCGTTCC TCATAACCAA TGTTGTCATT GAACTGGAGG AGGGGCAGAC CATCCTGATC
    CAGGGATCCA TGCTGCGAGC TTCAGATGTG GATGCCAGTG ATGACTACAT CTTCTTCAAT
    ATCACAAAGC CTCCACAGGC TGGGGAGATC ATGAAGAAGC CAGGGCCAGG ACTGATAGGC
    TATCCTGTCC CTGGCTTCCT TCAGAGGGAT TTGTTTAATG GAATCATTTA TTATCGTCAT
    TTTGGTGGAG AAATCTTTGA AGATTCTTTT CAATTTGTCC TGTGGGACAG CCATGAACCT
    CCAAATCTTT CAGTGCCACA GGTGGCAACA ATCCATGTAA CTCCAGTGGA TGACCAGCTT
    CCAAAAGAGG CTCCTGGAGT TTCTCGGCAT TTGGTTGTCA AGGAAACTGA GGTGGCCTAT
    ATAACTAAGA AACAGCTACA TTTTATAGAT TCAGAATCAT ATGACAGGGA GCTGGTCTAC
    ACGATAACTA CTCCTCCATT TTTCTCCTTC AGCCACAGAC ACTTGGATGC TGGGAAATTA
    TTTATGGTGG ACAGCATACC AAAAGTAGTT AAGAATCCTA CGGCCCTGGA GCTGAGGTCA
    TTCACTCAGC ATGCTGTGAA CTATATGAAA GTGGCCTACA TGCCCCCCAT GCAAGACATT
    GGTCCCCATT GCAGAGATGT CCAGTTCACA TTTTCTGTCA GTAACCAACA TGGCGGCACT
    TTGCATGGGA TCTGCTTTAA CATTACAATC CTCCCAGTGG ACAATCAAGT TCCTGAGGCG
    TTCACCAACC CTCTGAAAGT GACTGAGGGA GGTCAAAGCA TCATCAGCAC AGAGCACATT
    CTGATTTCTG ATGCAGATAC CAAGCTGGAC AATATTGACC TCTCCCTGCG GGAATTGCCT
    CTGCACGGAA GGGTGGAGCT GAATGGATTT CCTCTAAATT CAGGGGGCAC ATTTTCTTGG
    GGCGATCTCC ATACCTTAAA AGTTAGGTAT CAACATGATG GAACTGAAGT TCTTCAGGAT
    GACCTACTCT TGGAGGTCAC CGATGGCACA AATTCAGCAG AATTTGTTCT ACATGTTGAG
    GTATTCCCTG TCAACGATGA GCCACCAGTC TTAAAGGCTG ACCTCATGCC TGTCATGAAT
    TGCTCAGAGG GAGGAGAGGT GGTCATCACC TCTGAATACA TTTTTGCTAC TGATGTGGAC
    AGCGATAACT TGAAGTTGAT GTTTGTGATT GCTCGCGAAC CTCAGCATGG GGTGGTGAGG
    AGAGCTGGAG TCACAGTGGA TCAGTTCTCT CAGAGAGATG TTATCTCAGA GGCCGTGACA
    TACAAACACA CAGGTGGCGA GATTGGCATG ATGCCCTGTT TTGACACCAT TACATTGGTG
    GTTTCGGATG GAGAGGCTGG CCCTTTTGTG AATGGCTGTT GCTACAATGG ACCCAATCCA
    TCTGTTCCGC TTCATGCGTC CTTTCCAGTG TATGATCTCA ACATCACGGT ATACCCAGTA
    GACAACCAGC CACCTTCCGT TGCAATAGGT CCCATGTTTG TTGTAGATGA GGGCTGCTCA
    ACAGCCCTTA CTGTTAACCA TTTGTCCGCC ACTGACCCTG ACACTGCAGC AGATGACTTG
    GAATTTGTTT TGGTTTCTCC TCCTCAGTTT GGCTACCTCG AAAATATACT CCCTTCTGTG
    GGTTTTGAAA AAAGCAATAT TGGCATAAGT ATAGATTCAT TTCAGTGGAA AGACATGAAC
    GCTTTTCACA TTAACTATGT GCAGTCCAGG CATCTGAGGA TAGAACCAAC TGCCGACCAG
    TTCACGGTGT ACGTCACAGA TGGGAAGCAA CACTCCTTGG AGATACCATT TTCTATTATA
    ATCAACCCCA CAAATGATGA AGCTCCTGAC TTTGTAGTGC AGAATATTAC TGTGTGCGAG
    GGTCAGATGA AAGAGCTGGA CTCTTCCATC ATCAGCGCTG TGGACCTGGA CATTCCCCAG
    GATGCCCTGC TGTTCAGCAT CACTCAAAAG CCACGCCATG GCCTCCTCAT CGATAGGGGG
    TTTAGCAAAG ACTTCTCTGA GAATAAGCAG CCAGCCAACC CTCACCAGAA ACATGTACCT
    GTTCACAGTT TTTCCATGGA ACTCCTCAAG ACTGGAATGA GGTTGACGTA CATGCATGAT
    GACTCAGAGA GCCTTGCTGA TGATTTTACA ATCCGTTTGT CAGATGGGAA ACATAAGATA
    CTTAAAACCA TTTCAATAGA GGTCATCCCA GTTAATGATG AAAAACCAAT GCTGAGCAAG
    AAGGCTGAAA TTGCAATGAA TATGGGTGAA ACTCGTATTA TTTCCAGTGC TATTCTTTCA
    GCCATAGATG AAGACTCACC CAGGGAGAAG ATTTACTATG TATTTGAAAG GCTTCCCCAA
    AATGGGCAAC TTCAGCTTAA GATAGGGAGG GACTGGGTTC CTCTCTCCCC TGGCATGAAA
    TGCACTCAGG AGGAAGTGGA TCTGAACTTG CTGAGATACA CACACACCGG GGCAATGGAT
    TCCCAGAATC AAGATAGCTT CACCTTCTAC CTTTGGGATG GCAACAACAG GTCCCCTGCT
    CTTGACTGTC AAATCACCAT CAAGGACATG GAAAAAGGTG ATATTGTCAT CCTTACAAAA
    CCACTCGTGG TGTCTAAAGG TGATAGAGGT TTCTTAACAA CCACCACTCT CCTGGCTGTG
    GACGGAACAG ACAAGCCTGA GGAACTGCTC TATGTCATCA CCTCCCCTCC ACGATATGGC
    CAGATCGAAT ATGTTCACTA TCCTGGAGTT CCCATTACAA ACTTCAGCCA AATGGATATA
    GCGGGGCAGA CAGTTTGCTA TGTACACAAG AGCAAGGTGA CTGTCTCCAG TGACAGATTC
    AGATTCATCA TCAGCAATGG ACTGCGGACC GAGCACGGGG TGTTTGAGAT CACACTGGAG
    ACTGTGGACA GAGCCCTGCC TGTGGTAACC AGGAACAAGG GGTTGAGACT GGCCCAAGGG
    GCCGTGGGCC TGCTTTCCCC TGACCTCCTT CAGCTGACCG ACCCTGATAC ACCTGTGGAG
    AACCTCACCT TCCTCTTGGT TCAGCTTCCC CAGCATGGCC AGCTCTACCT GTGGGGGACA
    GGGCTACTTC AACACAATTT CACCCAGCAG GATGTGGACA GCAGGAATGT GGCCTATCGG
    CACTCAGGAG GGGACTCCCA GACTGACTGC TTTACTTTCA TGGCCACAGA CGGGACAAAC
    CAAGGCTTTA TTGTGAATGG GAGAGTATGG GAAGAACCTG TTTTATTCAC CATTCAGGTA
    GACCAATTGG ACAAAACAGC TCCTCGTATC ACACTCTTGC ATTCCCCTTC TCAAGTGGGG
    CTCCTGAAAA ATGGCTGCTA CGGGATTTAC ATCACTTCCC GCGTGTTGAA GGCATCAGAC
    CCTGACACTG AGGACGATCA GATCATCTTT AAAATTCTAC AAGGCCCAAA ACATGGACAT
    CTGGAGAACA CAACAACAGG TGAATTTATC CATGAGAAAT TTAGCCAAAA GGACTTAAAC
    AGTAAGACTA TTCTTTACAT CATAAACCCA TCTTTGGAAG TAAATTCAGA TACCGTGGAA
    TTTCAAATCA TGGACCCCAC AGGGAACTCG GCCACTCCTC AAATTTTGGA ACTGAAGTGG
    TCTCATATTG AATGGTCACA GACCGAATAT GAAGTCTGTG AGAATGTGGG TGTGTTGCCC
    TTGGAAATTA TCAGAAGGGG ATATTCCATG GACTCGGCCT TTGTGGGTAT AAAGGTCAAC
    CAAGTGTCAG CTGCAGATGG AAAAGATTTC ACCGTGATTC CATCTAAACT GATTCAGTTT
    GACCCAGGAA TGTCAACTAA GATGTGGAAT ATAGCAATTA CCTATGACGG ATTAGAGGAA
    GATGATGAGG TCTTTGAAGT AATTCTGAAC TCCCCTGTGA ATGCAGTTCT TGGCACAAAG
    ACAAAAGCTG CAGTGAAAAT TTTGGACTCA AAAGGAGGAC AATGCCATCC TTCATATTCC
    TCCAACCAAA GCAAGCACAG CACATGGGAG AAGGGCATTT GGCATCTGCT GCCCCCAGGG
    TCTTCCTCAT CCACCACTTC TGGTTCCTTT CATCTGGAAA GAAGACCTCT TCCATCTTCC
    ATGCAGCTAG CAGTCACCAG GGGAGACACC CTGCGGGGCT TTGATTCTAC AGATCTTTCT
    CAAAGGAAGC TTAGGACCCG TGGGAATGGC AAAACAGTTC GTCCATCCTC TGTTTATAGA
    AATGGAACAG ACATCATCTA TAATTATCAT GGGATAGTTT CCTTGAAACT AGAGGATGAC
    AGCTTCCCAA CTCACAAAAG GAAGGCCAAA GTATCCATCA TTAGTCAGCC ACAAAAGACA
    ATCAAAGTGG CAGAACTGCC TCAAGCAGAT AAGGTGGAAT CCACAACTGA CTCACACTTC
    CCCAGACAGG ACCAGTTGCC CTCATTTCCA AAGAACTGCA CTCTGGAATT AAAGGGACTC
    TTCCATTTTG AAGAAGGCAT CCAGAAGCTG TATCAGTGCA ATGGGATTGC CTGGAAAGCC
    TGGAGTCCCC AAACCAAGGA TGTGGAAGAA AAATCCTGTC CAGCCGGGTG GCACCAGCAC
    TCAGGCTACT GTCACATCTT GATCACAGAG CAGAAAGGCA CCTGGAATGC GGCTGCCCAA
    GCTTGCAGGG AACAATACCT GGGCAACCTT GTAACTGTAT TCTCCAGGCA GCACATGCGG
    TGGCTCTGGG ACATTGGTGA GAGAAAGTCC TTTTGGATAG GTTTGAACGA CCAAGTGCAT
    GCTGGCCACT GGGAGTGGAT CGGTGGTGAA CCTGTTGCCT TCACCAATGG GAGAAGAGGG
    CCCTCTCAAC GCTCCAAGCT TGGAAAGAGC TGTGTTTTGG TTCAAAGACA AGGGAAATGG
    CAAACAAAAG ATTGTAGGAG AGCCAAACCT CATAATTATG TGTGTTCCAG AAAACTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OPc88756
    Clone ID Related Accession (Same CDS sequence) XM_009456318.2 , XM_016960627.1 , XM_016960625.1 , XM_016960626.1
    Accession Version XM_016960627.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6540bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-01
    Organism Pan troglodytes(chimpanzee)
    Product FRAS1-related extracellular matrix protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006476.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGAACTCTC TGAGTTGGGG GGCTGCGAAT GCCGTGCTGC TGCTGCTCCT CCTGGCCTGG 
    GCCAGCCCCA CCTTCATCAG CATCAACCGT GGGGTGAGGG TGATGAAGGG CCACTCTGCC
    TTCCTGTCAG GAGATGACCT GAAGTTTGCC ATCCCTAAAG AGAAAGATGC CTGCAAAGTG
    GAAGTTGTGA TGAATGAGCC AATAACCCAG AGGGTTGGGA AACTCACTCC ACAGGTCTTT
    GACTGCCATT TCCTTCCCAA TGAAGTCAAG TATGTTCACA ATGGTTGTCC AATTCTTGAT
    GAAGACACAG TGAAGCTTAG ACTTTACAGA TTTACTGAAA GAGATACCTT CATAGAAACC
    TTTATCCTGT GGGTCTATCT CCTGGAACCA GACTGTAACA TCATCCATAT GAGTAACAAT
    GTGCTGGAGG TGCCTGAATT CAATGGCCTG TCCCAAGCGA TTGATAAAAA TCTGCTCAGA
    TTCGATTATG ATAGGATGGC TAGCCTGGAA TGTACGGTCA GCCTGGACAC TGCGAGAACT
    CGGCTGCCAG CCCATGGCCA GATGGTTCTC GGGGAGCCTC GACCAGAAGA ACCTCGTGGA
    GATCAGCCAC ACAGTTTTTT CCCAGAGTCC CAACTGAGAG CAAAACTGAA GTGTCCAGGT
    GGAAGCTGTA CCCCAGGATT AAAGAAAATA GGAAGCCTCA AAGTGAGCTG TGAGGAGTTC
    CTGCTGATGG GCCTTCGTTA TCAGCATCTG GATCCCCCTT CACCCAACAT TGATTATATC
    TCCATCCAAC TGGACCTCAC AGATACCAGG AGTAAAATTG TGTACAAGTC AGAGAGTGCG
    TGGCTGCCTG TCTATATCAG AGCTGGAATT CCGAATCAGA TTCCAAAGGC TGCATTCATG
    GCCATGTTTA TTCTGGAAGT GGATCAGTTC ATCCTGACCT CCTTGACTAC ATCAGTTCTG
    GACTGTGAAG AAGATGAGAC CCCTAAACCC TTGCTGGTGT TCAACATTAC TAAAGCCCCG
    CTCCAGGGCT ATGTGACTCA CCTGTTGGAT CACACCAGAC CAATCTCCTC ATTCACCTGG
    AAAGATCTCA GTGACATGCA GATCGCCTAT CAGCCACCAA ACAGCAGCCA TTCTGAGAGG
    AGACATGATG AGGTAGAATT GGAGGTATAC GACTTCTTCT TTGAAAGGAG TGCACCTATG
    ACAGTCCACA TCTCCATCAG AACAGCAGAT ACAAATGCCC CCCGTGTATC CTGGAATACA
    GGTCTGAATC TCCTTGAGGG GCAGTCTCGA GCCATCACTT GGGAACAGTT TCAGGTTGTC
    GACAATGACG ACATCGGTGC TGTCCGGCTA GTCACCGTTG GTGGCCTGCA GCATGGATGG
    CTGACTTTAA GAGGGGGGAA AGGATTTCTC TTCACCGTGG CTGACCTCCA GGCTGGAGTT
    GTTCGCTATC ATCATGATGA CAGCGACTCC ACCAAAGACT TCGTGGTCTT CCGGATATTT
    GATGGCCATC ACAGCATCCG TCACAAATTC CCCATCAACG TCTTGCCCAA AGATGATAGT
    CCCCCGTTCC TCATAACCAA TGTTGTCATT GAACTGGAGG AGGGGCAGAC CATCCTGATC
    CAGGGATCCA TGCTGCGAGC TTCAGATGTG GATGCCAGTG ATGACTACAT CTTCTTCAAT
    ATCACAAAGC CTCCACAGGC TGGGGAGATC ATGAAGAAGC CAGGGCCAGG ACTGATAGGC
    TATCCTGTCC CTGGCTTCCT TCAGAGGGAT TTGTTTAATG GAATCATTTA TTATCGTCAT
    TTTGGTGGAG AAATCTTTGA AGATTCTTTT CAATTTGTCC TGTGGGACAG CCATGAACCT
    CCAAATCTTT CAGTGCCACA GGTGGCAACA ATCCATGTAA CTCCAGTGGA TGACCAGCTT
    CCAAAAGAGG CTCCTGGAGT TTCTCGGCAT TTGGTTGTCA AGGAAACTGA GGTGGCCTAT
    ATAACTAAGA AACAGCTACA TTTTATAGAT TCAGAATCAT ATGACAGGGA GCTGGTCTAC
    ACGATAACTA CTCCTCCATT TTTCTCCTTC AGCCACAGAC ACTTGGATGC TGGGAAATTA
    TTTATGGTGG ACAGCATACC AAAAGTAGTT AAGAATCCTA CGGCCCTGGA GCTGAGGTCA
    TTCACTCAGC ATGCTGTGAA CTATATGAAA GTGGCCTACA TGCCCCCCAT GCAAGACATT
    GGTCCCCATT GCAGAGATGT CCAGTTCACA TTTTCTGTCA GTAACCAACA TGGCGGCACT
    TTGCATGGGA TCTGCTTTAA CATTACAATC CTCCCAGTGG ACAATCAAGT TCCTGAGGCG
    TTCACCAACC CTCTGAAAGT GACTGAGGGA GGTCAAAGCA TCATCAGCAC AGAGCACATT
    CTGATTTCTG ATGCAGATAC CAAGCTGGAC AATATTGACC TCTCCCTGCG GGAATTGCCT
    CTGCACGGAA GGGTGGAGCT GAATGGATTT CCTCTAAATT CAGGGGGCAC ATTTTCTTGG
    GGCGATCTCC ATACCTTAAA AGTTAGGTAT CAACATGATG GAACTGAAGT TCTTCAGGAT
    GACCTACTCT TGGAGGTCAC CGATGGCACA AATTCAGCAG AATTTGTTCT ACATGTTGAG
    GTATTCCCTG TCAACGATGA GCCACCAGTC TTAAAGGCTG ACCTCATGCC TGTCATGAAT
    TGCTCAGAGG GAGGAGAGGT GGTCATCACC TCTGAATACA TTTTTGCTAC TGATGTGGAC
    AGCGATAACT TGAAGTTGAT GTTTGTGATT GCTCGCGAAC CTCAGCATGG GGTGGTGAGG
    AGAGCTGGAG TCACAGTGGA TCAGTTCTCT CAGAGAGATG TTATCTCAGA GGCCGTGACA
    TACAAACACA CAGGTGGCGA GATTGGCATG ATGCCCTGTT TTGACACCAT TACATTGGTG
    GTTTCGGATG GAGAGGCTGG CCCTTTTGTG AATGGCTGTT GCTACAATGG ACCCAATCCA
    TCTGTTCCGC TTCATGCGTC CTTTCCAGTG TATGATCTCA ACATCACGGT ATACCCAGTA
    GACAACCAGC CACCTTCCGT TGCAATAGGT CCCATGTTTG TTGTAGATGA GGGCTGCTCA
    ACAGCCCTTA CTGTTAACCA TTTGTCCGCC ACTGACCCTG ACACTGCAGC AGATGACTTG
    GAATTTGTTT TGGTTTCTCC TCCTCAGTTT GGCTACCTCG AAAATATACT CCCTTCTGTG
    GGTTTTGAAA AAAGCAATAT TGGCATAAGT ATAGATTCAT TTCAGTGGAA AGACATGAAC
    GCTTTTCACA TTAACTATGT GCAGTCCAGG CATCTGAGGA TAGAACCAAC TGCCGACCAG
    TTCACGGTGT ACGTCACAGA TGGGAAGCAA CACTCCTTGG AGATACCATT TTCTATTATA
    ATCAACCCCA CAAATGATGA AGCTCCTGAC TTTGTAGTGC AGAATATTAC TGTGTGCGAG
    GGTCAGATGA AAGAGCTGGA CTCTTCCATC ATCAGCGCTG TGGACCTGGA CATTCCCCAG
    GATGCCCTGC TGTTCAGCAT CACTCAAAAG CCACGCCATG GCCTCCTCAT CGATAGGGGG
    TTTAGCAAAG ACTTCTCTGA GAATAAGCAG CCAGCCAACC CTCACCAGAA ACATGTACCT
    GTTCACAGTT TTTCCATGGA ACTCCTCAAG ACTGGAATGA GGTTGACGTA CATGCATGAT
    GACTCAGAGA GCCTTGCTGA TGATTTTACA ATCCGTTTGT CAGATGGGAA ACATAAGATA
    CTTAAAACCA TTTCAATAGA GGTCATCCCA GTTAATGATG AAAAACCAAT GCTGAGCAAG
    AAGGCTGAAA TTGCAATGAA TATGGGTGAA ACTCGTATTA TTTCCAGTGC TATTCTTTCA
    GCCATAGATG AAGACTCACC CAGGGAGAAG ATTTACTATG TATTTGAAAG GCTTCCCCAA
    AATGGGCAAC TTCAGCTTAA GATAGGGAGG GACTGGGTTC CTCTCTCCCC TGGCATGAAA
    TGCACTCAGG AGGAAGTGGA TCTGAACTTG CTGAGATACA CACACACCGG GGCAATGGAT
    TCCCAGAATC AAGATAGCTT CACCTTCTAC CTTTGGGATG GCAACAACAG GTCCCCTGCT
    CTTGACTGTC AAATCACCAT CAAGGACATG GAAAAAGGTG ATATTGTCAT CCTTACAAAA
    CCACTCGTGG TGTCTAAAGG TGATAGAGGT TTCTTAACAA CCACCACTCT CCTGGCTGTG
    GACGGAACAG ACAAGCCTGA GGAACTGCTC TATGTCATCA CCTCCCCTCC ACGATATGGC
    CAGATCGAAT ATGTTCACTA TCCTGGAGTT CCCATTACAA ACTTCAGCCA AATGGATATA
    GCGGGGCAGA CAGTTTGCTA TGTACACAAG AGCAAGGTGA CTGTCTCCAG TGACAGATTC
    AGATTCATCA TCAGCAATGG ACTGCGGACC GAGCACGGGG TGTTTGAGAT CACACTGGAG
    ACTGTGGACA GAGCCCTGCC TGTGGTAACC AGGAACAAGG GGTTGAGACT GGCCCAAGGG
    GCCGTGGGCC TGCTTTCCCC TGACCTCCTT CAGCTGACCG ACCCTGATAC ACCTGTGGAG
    AACCTCACCT TCCTCTTGGT TCAGCTTCCC CAGCATGGCC AGCTCTACCT GTGGGGGACA
    GGGCTACTTC AACACAATTT CACCCAGCAG GATGTGGACA GCAGGAATGT GGCCTATCGG
    CACTCAGGAG GGGACTCCCA GACTGACTGC TTTACTTTCA TGGCCACAGA CGGGACAAAC
    CAAGGCTTTA TTGTGAATGG GAGAGTATGG GAAGAACCTG TTTTATTCAC CATTCAGGTA
    GACCAATTGG ACAAAACAGC TCCTCGTATC ACACTCTTGC ATTCCCCTTC TCAAGTGGGG
    CTCCTGAAAA ATGGCTGCTA CGGGATTTAC ATCACTTCCC GCGTGTTGAA GGCATCAGAC
    CCTGACACTG AGGACGATCA GATCATCTTT AAAATTCTAC AAGGCCCAAA ACATGGACAT
    CTGGAGAACA CAACAACAGG TGAATTTATC CATGAGAAAT TTAGCCAAAA GGACTTAAAC
    AGTAAGACTA TTCTTTACAT CATAAACCCA TCTTTGGAAG TAAATTCAGA TACCGTGGAA
    TTTCAAATCA TGGACCCCAC AGGGAACTCG GCCACTCCTC AAATTTTGGA ACTGAAGTGG
    TCTCATATTG AATGGTCACA GACCGAATAT GAAGTCTGTG AGAATGTGGG TGTGTTGCCC
    TTGGAAATTA TCAGAAGGGG ATATTCCATG GACTCGGCCT TTGTGGGTAT AAAGGTCAAC
    CAAGTGTCAG CTGCAGATGG AAAAGATTTC ACCGTGATTC CATCTAAACT GATTCAGTTT
    GACCCAGGAA TGTCAACTAA GATGTGGAAT ATAGCAATTA CCTATGACGG ATTAGAGGAA
    GATGATGAGG TCTTTGAAGT AATTCTGAAC TCCCCTGTGA ATGCAGTTCT TGGCACAAAG
    ACAAAAGCTG CAGTGAAAAT TTTGGACTCA AAAGGAGGAC AATGCCATCC TTCATATTCC
    TCCAACCAAA GCAAGCACAG CACATGGGAG AAGGGCATTT GGCATCTGCT GCCCCCAGGG
    TCTTCCTCAT CCACCACTTC TGGTTCCTTT CATCTGGAAA GAAGACCTCT TCCATCTTCC
    ATGCAGCTAG CAGTCACCAG GGGAGACACC CTGCGGGGCT TTGATTCTAC AGATCTTTCT
    CAAAGGAAGC TTAGGACCCG TGGGAATGGC AAAACAGTTC GTCCATCCTC TGTTTATAGA
    AATGGAACAG ACATCATCTA TAATTATCAT GGGATAGTTT CCTTGAAACT AGAGGATGAC
    AGCTTCCCAA CTCACAAAAG GAAGGCCAAA GTATCCATCA TTAGTCAGCC ACAAAAGACA
    ATCAAAGTGG CAGAACTGCC TCAAGCAGAT AAGGTGGAAT CCACAACTGA CTCACACTTC
    CCCAGACAGG ACCAGTTGCC CTCATTTCCA AAGAACTGCA CTCTGGAATT AAAGGGACTC
    TTCCATTTTG AAGAAGGCAT CCAGAAGCTG TATCAGTGCA ATGGGATTGC CTGGAAAGCC
    TGGAGTCCCC AAACCAAGGA TGTGGAAGAA AAATCCTGTC CAGCCGGGTG GCACCAGCAC
    TCAGGCTACT GTCACATCTT GATCACAGAG CAGAAAGGCA CCTGGAATGC GGCTGCCCAA
    GCTTGCAGGG AACAATACCT GGGCAACCTT GTAACTGTAT TCTCCAGGCA GCACATGCGG
    TGGCTCTGGG ACATTGGTGA GAGAAAGTCC TTTTGGATAG GTTTGAACGA CCAAGTGCAT
    GCTGGCCACT GGGAGTGGAT CGGTGGTGAA CCTGTTGCCT TCACCAATGG GAGAAGAGGG
    CCCTCTCAAC GCTCCAAGCT TGGAAAGAGC TGTGTTTTGG TTCAAAGACA AGGGAAATGG
    CAAACAAAAG ATTGTAGGAG AGCCAAACCT CATAATTATG TGTGTTCCAG AAAACTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016816116.1
    CDS1601..8140
    Translation

    Target ORF information:

    RefSeq Version XM_016960627.1
    Organism Pan troglodytes(chimpanzee)
    Definition Pan troglodytes FRAS1 related extracellular matrix 1 (FREM1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_016960627.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGAACTCTC TGAGTTGGGG GGCTGCGAAT GCCGTGCTGC TGCTGCTCCT CCTGGCCTGG 
    GCCAGCCCCA CCTTCATCAG CATCAACCGT GGGGTGAGGG TGATGAAGGG CCACTCTGCC
    TTCCTGTCAG GAGATGACCT GAAGTTTGCC ATCCCTAAAG AGAAAGATGC CTGCAAAGTG
    GAAGTTGTGA TGAATGAGCC AATAACCCAG AGGGTTGGGA AACTCACTCC ACAGGTCTTT
    GACTGCCATT TCCTTCCCAA TGAAGTCAAG TATGTTCACA ATGGTTGTCC AATTCTTGAT
    GAAGACACAG TGAAGCTTAG ACTTTACAGA TTTACTGAAA GAGATACCTT CATAGAAACC
    TTTATCCTGT GGGTCTATCT CCTGGAACCA GACTGTAACA TCATCCATAT GAGTAACAAT
    GTGCTGGAGG TGCCTGAATT CAATGGCCTG TCCCAAGCGA TTGATAAAAA TCTGCTCAGA
    TTCGATTATG ATAGGATGGC TAGCCTGGAA TGTACGGTCA GCCTGGACAC TGCGAGAACT
    CGGCTGCCAG CCCATGGCCA GATGGTTCTC GGGGAGCCTC GACCAGAAGA ACCTCGTGGA
    GATCAGCCAC ACAGTTTTTT CCCAGAGTCC CAACTGAGAG CAAAACTGAA GTGTCCAGGT
    GGAAGCTGTA CCCCAGGATT AAAGAAAATA GGAAGCCTCA AAGTGAGCTG TGAGGAGTTC
    CTGCTGATGG GCCTTCGTTA TCAGCATCTG GATCCCCCTT CACCCAACAT TGATTATATC
    TCCATCCAAC TGGACCTCAC AGATACCAGG AGTAAAATTG TGTACAAGTC AGAGAGTGCG
    TGGCTGCCTG TCTATATCAG AGCTGGAATT CCGAATCAGA TTCCAAAGGC TGCATTCATG
    GCCATGTTTA TTCTGGAAGT GGATCAGTTC ATCCTGACCT CCTTGACTAC ATCAGTTCTG
    GACTGTGAAG AAGATGAGAC CCCTAAACCC TTGCTGGTGT TCAACATTAC TAAAGCCCCG
    CTCCAGGGCT ATGTGACTCA CCTGTTGGAT CACACCAGAC CAATCTCCTC ATTCACCTGG
    AAAGATCTCA GTGACATGCA GATCGCCTAT CAGCCACCAA ACAGCAGCCA TTCTGAGAGG
    AGACATGATG AGGTAGAATT GGAGGTATAC GACTTCTTCT TTGAAAGGAG TGCACCTATG
    ACAGTCCACA TCTCCATCAG AACAGCAGAT ACAAATGCCC CCCGTGTATC CTGGAATACA
    GGTCTGAATC TCCTTGAGGG GCAGTCTCGA GCCATCACTT GGGAACAGTT TCAGGTTGTC
    GACAATGACG ACATCGGTGC TGTCCGGCTA GTCACCGTTG GTGGCCTGCA GCATGGATGG
    CTGACTTTAA GAGGGGGGAA AGGATTTCTC TTCACCGTGG CTGACCTCCA GGCTGGAGTT
    GTTCGCTATC ATCATGATGA CAGCGACTCC ACCAAAGACT TCGTGGTCTT CCGGATATTT
    GATGGCCATC ACAGCATCCG TCACAAATTC CCCATCAACG TCTTGCCCAA AGATGATAGT
    CCCCCGTTCC TCATAACCAA TGTTGTCATT GAACTGGAGG AGGGGCAGAC CATCCTGATC
    CAGGGATCCA TGCTGCGAGC TTCAGATGTG GATGCCAGTG ATGACTACAT CTTCTTCAAT
    ATCACAAAGC CTCCACAGGC TGGGGAGATC ATGAAGAAGC CAGGGCCAGG ACTGATAGGC
    TATCCTGTCC CTGGCTTCCT TCAGAGGGAT TTGTTTAATG GAATCATTTA TTATCGTCAT
    TTTGGTGGAG AAATCTTTGA AGATTCTTTT CAATTTGTCC TGTGGGACAG CCATGAACCT
    CCAAATCTTT CAGTGCCACA GGTGGCAACA ATCCATGTAA CTCCAGTGGA TGACCAGCTT
    CCAAAAGAGG CTCCTGGAGT TTCTCGGCAT TTGGTTGTCA AGGAAACTGA GGTGGCCTAT
    ATAACTAAGA AACAGCTACA TTTTATAGAT TCAGAATCAT ATGACAGGGA GCTGGTCTAC
    ACGATAACTA CTCCTCCATT TTTCTCCTTC AGCCACAGAC ACTTGGATGC TGGGAAATTA
    TTTATGGTGG ACAGCATACC AAAAGTAGTT AAGAATCCTA CGGCCCTGGA GCTGAGGTCA
    TTCACTCAGC ATGCTGTGAA CTATATGAAA GTGGCCTACA TGCCCCCCAT GCAAGACATT
    GGTCCCCATT GCAGAGATGT CCAGTTCACA TTTTCTGTCA GTAACCAACA TGGCGGCACT
    TTGCATGGGA TCTGCTTTAA CATTACAATC CTCCCAGTGG ACAATCAAGT TCCTGAGGCG
    TTCACCAACC CTCTGAAAGT GACTGAGGGA GGTCAAAGCA TCATCAGCAC AGAGCACATT
    CTGATTTCTG ATGCAGATAC CAAGCTGGAC AATATTGACC TCTCCCTGCG GGAATTGCCT
    CTGCACGGAA GGGTGGAGCT GAATGGATTT CCTCTAAATT CAGGGGGCAC ATTTTCTTGG
    GGCGATCTCC ATACCTTAAA AGTTAGGTAT CAACATGATG GAACTGAAGT TCTTCAGGAT
    GACCTACTCT TGGAGGTCAC CGATGGCACA AATTCAGCAG AATTTGTTCT ACATGTTGAG
    GTATTCCCTG TCAACGATGA GCCACCAGTC TTAAAGGCTG ACCTCATGCC TGTCATGAAT
    TGCTCAGAGG GAGGAGAGGT GGTCATCACC TCTGAATACA TTTTTGCTAC TGATGTGGAC
    AGCGATAACT TGAAGTTGAT GTTTGTGATT GCTCGCGAAC CTCAGCATGG GGTGGTGAGG
    AGAGCTGGAG TCACAGTGGA TCAGTTCTCT CAGAGAGATG TTATCTCAGA GGCCGTGACA
    TACAAACACA CAGGTGGCGA GATTGGCATG ATGCCCTGTT TTGACACCAT TACATTGGTG
    GTTTCGGATG GAGAGGCTGG CCCTTTTGTG AATGGCTGTT GCTACAATGG ACCCAATCCA
    TCTGTTCCGC TTCATGCGTC CTTTCCAGTG TATGATCTCA ACATCACGGT ATACCCAGTA
    GACAACCAGC CACCTTCCGT TGCAATAGGT CCCATGTTTG TTGTAGATGA GGGCTGCTCA
    ACAGCCCTTA CTGTTAACCA TTTGTCCGCC ACTGACCCTG ACACTGCAGC AGATGACTTG
    GAATTTGTTT TGGTTTCTCC TCCTCAGTTT GGCTACCTCG AAAATATACT CCCTTCTGTG
    GGTTTTGAAA AAAGCAATAT TGGCATAAGT ATAGATTCAT TTCAGTGGAA AGACATGAAC
    GCTTTTCACA TTAACTATGT GCAGTCCAGG CATCTGAGGA TAGAACCAAC TGCCGACCAG
    TTCACGGTGT ACGTCACAGA TGGGAAGCAA CACTCCTTGG AGATACCATT TTCTATTATA
    ATCAACCCCA CAAATGATGA AGCTCCTGAC TTTGTAGTGC AGAATATTAC TGTGTGCGAG
    GGTCAGATGA AAGAGCTGGA CTCTTCCATC ATCAGCGCTG TGGACCTGGA CATTCCCCAG
    GATGCCCTGC TGTTCAGCAT CACTCAAAAG CCACGCCATG GCCTCCTCAT CGATAGGGGG
    TTTAGCAAAG ACTTCTCTGA GAATAAGCAG CCAGCCAACC CTCACCAGAA ACATGTACCT
    GTTCACAGTT TTTCCATGGA ACTCCTCAAG ACTGGAATGA GGTTGACGTA CATGCATGAT
    GACTCAGAGA GCCTTGCTGA TGATTTTACA ATCCGTTTGT CAGATGGGAA ACATAAGATA
    CTTAAAACCA TTTCAATAGA GGTCATCCCA GTTAATGATG AAAAACCAAT GCTGAGCAAG
    AAGGCTGAAA TTGCAATGAA TATGGGTGAA ACTCGTATTA TTTCCAGTGC TATTCTTTCA
    GCCATAGATG AAGACTCACC CAGGGAGAAG ATTTACTATG TATTTGAAAG GCTTCCCCAA
    AATGGGCAAC TTCAGCTTAA GATAGGGAGG GACTGGGTTC CTCTCTCCCC TGGCATGAAA
    TGCACTCAGG AGGAAGTGGA TCTGAACTTG CTGAGATACA CACACACCGG GGCAATGGAT
    TCCCAGAATC AAGATAGCTT CACCTTCTAC CTTTGGGATG GCAACAACAG GTCCCCTGCT
    CTTGACTGTC AAATCACCAT CAAGGACATG GAAAAAGGTG ATATTGTCAT CCTTACAAAA
    CCACTCGTGG TGTCTAAAGG TGATAGAGGT TTCTTAACAA CCACCACTCT CCTGGCTGTG
    GACGGAACAG ACAAGCCTGA GGAACTGCTC TATGTCATCA CCTCCCCTCC ACGATATGGC
    CAGATCGAAT ATGTTCACTA TCCTGGAGTT CCCATTACAA ACTTCAGCCA AATGGATATA
    GCGGGGCAGA CAGTTTGCTA TGTACACAAG AGCAAGGTGA CTGTCTCCAG TGACAGATTC
    AGATTCATCA TCAGCAATGG ACTGCGGACC GAGCACGGGG TGTTTGAGAT CACACTGGAG
    ACTGTGGACA GAGCCCTGCC TGTGGTAACC AGGAACAAGG GGTTGAGACT GGCCCAAGGG
    GCCGTGGGCC TGCTTTCCCC TGACCTCCTT CAGCTGACCG ACCCTGATAC ACCTGTGGAG
    AACCTCACCT TCCTCTTGGT TCAGCTTCCC CAGCATGGCC AGCTCTACCT GTGGGGGACA
    GGGCTACTTC AACACAATTT CACCCAGCAG GATGTGGACA GCAGGAATGT GGCCTATCGG
    CACTCAGGAG GGGACTCCCA GACTGACTGC TTTACTTTCA TGGCCACAGA CGGGACAAAC
    CAAGGCTTTA TTGTGAATGG GAGAGTATGG GAAGAACCTG TTTTATTCAC CATTCAGGTA
    GACCAATTGG ACAAAACAGC TCCTCGTATC ACACTCTTGC ATTCCCCTTC TCAAGTGGGG
    CTCCTGAAAA ATGGCTGCTA CGGGATTTAC ATCACTTCCC GCGTGTTGAA GGCATCAGAC
    CCTGACACTG AGGACGATCA GATCATCTTT AAAATTCTAC AAGGCCCAAA ACATGGACAT
    CTGGAGAACA CAACAACAGG TGAATTTATC CATGAGAAAT TTAGCCAAAA GGACTTAAAC
    AGTAAGACTA TTCTTTACAT CATAAACCCA TCTTTGGAAG TAAATTCAGA TACCGTGGAA
    TTTCAAATCA TGGACCCCAC AGGGAACTCG GCCACTCCTC AAATTTTGGA ACTGAAGTGG
    TCTCATATTG AATGGTCACA GACCGAATAT GAAGTCTGTG AGAATGTGGG TGTGTTGCCC
    TTGGAAATTA TCAGAAGGGG ATATTCCATG GACTCGGCCT TTGTGGGTAT AAAGGTCAAC
    CAAGTGTCAG CTGCAGATGG AAAAGATTTC ACCGTGATTC CATCTAAACT GATTCAGTTT
    GACCCAGGAA TGTCAACTAA GATGTGGAAT ATAGCAATTA CCTATGACGG ATTAGAGGAA
    GATGATGAGG TCTTTGAAGT AATTCTGAAC TCCCCTGTGA ATGCAGTTCT TGGCACAAAG
    ACAAAAGCTG CAGTGAAAAT TTTGGACTCA AAAGGAGGAC AATGCCATCC TTCATATTCC
    TCCAACCAAA GCAAGCACAG CACATGGGAG AAGGGCATTT GGCATCTGCT GCCCCCAGGG
    TCTTCCTCAT CCACCACTTC TGGTTCCTTT CATCTGGAAA GAAGACCTCT TCCATCTTCC
    ATGCAGCTAG CAGTCACCAG GGGAGACACC CTGCGGGGCT TTGATTCTAC AGATCTTTCT
    CAAAGGAAGC TTAGGACCCG TGGGAATGGC AAAACAGTTC GTCCATCCTC TGTTTATAGA
    AATGGAACAG ACATCATCTA TAATTATCAT GGGATAGTTT CCTTGAAACT AGAGGATGAC
    AGCTTCCCAA CTCACAAAAG GAAGGCCAAA GTATCCATCA TTAGTCAGCC ACAAAAGACA
    ATCAAAGTGG CAGAACTGCC TCAAGCAGAT AAGGTGGAAT CCACAACTGA CTCACACTTC
    CCCAGACAGG ACCAGTTGCC CTCATTTCCA AAGAACTGCA CTCTGGAATT AAAGGGACTC
    TTCCATTTTG AAGAAGGCAT CCAGAAGCTG TATCAGTGCA ATGGGATTGC CTGGAAAGCC
    TGGAGTCCCC AAACCAAGGA TGTGGAAGAA AAATCCTGTC CAGCCGGGTG GCACCAGCAC
    TCAGGCTACT GTCACATCTT GATCACAGAG CAGAAAGGCA CCTGGAATGC GGCTGCCCAA
    GCTTGCAGGG AACAATACCT GGGCAACCTT GTAACTGTAT TCTCCAGGCA GCACATGCGG
    TGGCTCTGGG ACATTGGTGA GAGAAAGTCC TTTTGGATAG GTTTGAACGA CCAAGTGCAT
    GCTGGCCACT GGGAGTGGAT CGGTGGTGAA CCTGTTGCCT TCACCAATGG GAGAAGAGGG
    CCCTCTCAAC GCTCCAAGCT TGGAAAGAGC TGTGTTTTGG TTCAAAGACA AGGGAAATGG
    CAAACAAAAG ATTGTAGGAG AGCCAAACCT CATAATTATG TGTGTTCCAG AAAACTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OPc88756
    Clone ID Related Accession (Same CDS sequence) XM_009456318.2 , XM_016960627.1 , XM_016960625.1 , XM_016960626.1
    Accession Version XM_016960625.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6540bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-01
    Organism Pan troglodytes(chimpanzee)
    Product FRAS1-related extracellular matrix protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006476.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGAACTCTC TGAGTTGGGG GGCTGCGAAT GCCGTGCTGC TGCTGCTCCT CCTGGCCTGG 
    GCCAGCCCCA CCTTCATCAG CATCAACCGT GGGGTGAGGG TGATGAAGGG CCACTCTGCC
    TTCCTGTCAG GAGATGACCT GAAGTTTGCC ATCCCTAAAG AGAAAGATGC CTGCAAAGTG
    GAAGTTGTGA TGAATGAGCC AATAACCCAG AGGGTTGGGA AACTCACTCC ACAGGTCTTT
    GACTGCCATT TCCTTCCCAA TGAAGTCAAG TATGTTCACA ATGGTTGTCC AATTCTTGAT
    GAAGACACAG TGAAGCTTAG ACTTTACAGA TTTACTGAAA GAGATACCTT CATAGAAACC
    TTTATCCTGT GGGTCTATCT CCTGGAACCA GACTGTAACA TCATCCATAT GAGTAACAAT
    GTGCTGGAGG TGCCTGAATT CAATGGCCTG TCCCAAGCGA TTGATAAAAA TCTGCTCAGA
    TTCGATTATG ATAGGATGGC TAGCCTGGAA TGTACGGTCA GCCTGGACAC TGCGAGAACT
    CGGCTGCCAG CCCATGGCCA GATGGTTCTC GGGGAGCCTC GACCAGAAGA ACCTCGTGGA
    GATCAGCCAC ACAGTTTTTT CCCAGAGTCC CAACTGAGAG CAAAACTGAA GTGTCCAGGT
    GGAAGCTGTA CCCCAGGATT AAAGAAAATA GGAAGCCTCA AAGTGAGCTG TGAGGAGTTC
    CTGCTGATGG GCCTTCGTTA TCAGCATCTG GATCCCCCTT CACCCAACAT TGATTATATC
    TCCATCCAAC TGGACCTCAC AGATACCAGG AGTAAAATTG TGTACAAGTC AGAGAGTGCG
    TGGCTGCCTG TCTATATCAG AGCTGGAATT CCGAATCAGA TTCCAAAGGC TGCATTCATG
    GCCATGTTTA TTCTGGAAGT GGATCAGTTC ATCCTGACCT CCTTGACTAC ATCAGTTCTG
    GACTGTGAAG AAGATGAGAC CCCTAAACCC TTGCTGGTGT TCAACATTAC TAAAGCCCCG
    CTCCAGGGCT ATGTGACTCA CCTGTTGGAT CACACCAGAC CAATCTCCTC ATTCACCTGG
    AAAGATCTCA GTGACATGCA GATCGCCTAT CAGCCACCAA ACAGCAGCCA TTCTGAGAGG
    AGACATGATG AGGTAGAATT GGAGGTATAC GACTTCTTCT TTGAAAGGAG TGCACCTATG
    ACAGTCCACA TCTCCATCAG AACAGCAGAT ACAAATGCCC CCCGTGTATC CTGGAATACA
    GGTCTGAATC TCCTTGAGGG GCAGTCTCGA GCCATCACTT GGGAACAGTT TCAGGTTGTC
    GACAATGACG ACATCGGTGC TGTCCGGCTA GTCACCGTTG GTGGCCTGCA GCATGGATGG
    CTGACTTTAA GAGGGGGGAA AGGATTTCTC TTCACCGTGG CTGACCTCCA GGCTGGAGTT
    GTTCGCTATC ATCATGATGA CAGCGACTCC ACCAAAGACT TCGTGGTCTT CCGGATATTT
    GATGGCCATC ACAGCATCCG TCACAAATTC CCCATCAACG TCTTGCCCAA AGATGATAGT
    CCCCCGTTCC TCATAACCAA TGTTGTCATT GAACTGGAGG AGGGGCAGAC CATCCTGATC
    CAGGGATCCA TGCTGCGAGC TTCAGATGTG GATGCCAGTG ATGACTACAT CTTCTTCAAT
    ATCACAAAGC CTCCACAGGC TGGGGAGATC ATGAAGAAGC CAGGGCCAGG ACTGATAGGC
    TATCCTGTCC CTGGCTTCCT TCAGAGGGAT TTGTTTAATG GAATCATTTA TTATCGTCAT
    TTTGGTGGAG AAATCTTTGA AGATTCTTTT CAATTTGTCC TGTGGGACAG CCATGAACCT
    CCAAATCTTT CAGTGCCACA GGTGGCAACA ATCCATGTAA CTCCAGTGGA TGACCAGCTT
    CCAAAAGAGG CTCCTGGAGT TTCTCGGCAT TTGGTTGTCA AGGAAACTGA GGTGGCCTAT
    ATAACTAAGA AACAGCTACA TTTTATAGAT TCAGAATCAT ATGACAGGGA GCTGGTCTAC
    ACGATAACTA CTCCTCCATT TTTCTCCTTC AGCCACAGAC ACTTGGATGC TGGGAAATTA
    TTTATGGTGG ACAGCATACC AAAAGTAGTT AAGAATCCTA CGGCCCTGGA GCTGAGGTCA
    TTCACTCAGC ATGCTGTGAA CTATATGAAA GTGGCCTACA TGCCCCCCAT GCAAGACATT
    GGTCCCCATT GCAGAGATGT CCAGTTCACA TTTTCTGTCA GTAACCAACA TGGCGGCACT
    TTGCATGGGA TCTGCTTTAA CATTACAATC CTCCCAGTGG ACAATCAAGT TCCTGAGGCG
    TTCACCAACC CTCTGAAAGT GACTGAGGGA GGTCAAAGCA TCATCAGCAC AGAGCACATT
    CTGATTTCTG ATGCAGATAC CAAGCTGGAC AATATTGACC TCTCCCTGCG GGAATTGCCT
    CTGCACGGAA GGGTGGAGCT GAATGGATTT CCTCTAAATT CAGGGGGCAC ATTTTCTTGG
    GGCGATCTCC ATACCTTAAA AGTTAGGTAT CAACATGATG GAACTGAAGT TCTTCAGGAT
    GACCTACTCT TGGAGGTCAC CGATGGCACA AATTCAGCAG AATTTGTTCT ACATGTTGAG
    GTATTCCCTG TCAACGATGA GCCACCAGTC TTAAAGGCTG ACCTCATGCC TGTCATGAAT
    TGCTCAGAGG GAGGAGAGGT GGTCATCACC TCTGAATACA TTTTTGCTAC TGATGTGGAC
    AGCGATAACT TGAAGTTGAT GTTTGTGATT GCTCGCGAAC CTCAGCATGG GGTGGTGAGG
    AGAGCTGGAG TCACAGTGGA TCAGTTCTCT CAGAGAGATG TTATCTCAGA GGCCGTGACA
    TACAAACACA CAGGTGGCGA GATTGGCATG ATGCCCTGTT TTGACACCAT TACATTGGTG
    GTTTCGGATG GAGAGGCTGG CCCTTTTGTG AATGGCTGTT GCTACAATGG ACCCAATCCA
    TCTGTTCCGC TTCATGCGTC CTTTCCAGTG TATGATCTCA ACATCACGGT ATACCCAGTA
    GACAACCAGC CACCTTCCGT TGCAATAGGT CCCATGTTTG TTGTAGATGA GGGCTGCTCA
    ACAGCCCTTA CTGTTAACCA TTTGTCCGCC ACTGACCCTG ACACTGCAGC AGATGACTTG
    GAATTTGTTT TGGTTTCTCC TCCTCAGTTT GGCTACCTCG AAAATATACT CCCTTCTGTG
    GGTTTTGAAA AAAGCAATAT TGGCATAAGT ATAGATTCAT TTCAGTGGAA AGACATGAAC
    GCTTTTCACA TTAACTATGT GCAGTCCAGG CATCTGAGGA TAGAACCAAC TGCCGACCAG
    TTCACGGTGT ACGTCACAGA TGGGAAGCAA CACTCCTTGG AGATACCATT TTCTATTATA
    ATCAACCCCA CAAATGATGA AGCTCCTGAC TTTGTAGTGC AGAATATTAC TGTGTGCGAG
    GGTCAGATGA AAGAGCTGGA CTCTTCCATC ATCAGCGCTG TGGACCTGGA CATTCCCCAG
    GATGCCCTGC TGTTCAGCAT CACTCAAAAG CCACGCCATG GCCTCCTCAT CGATAGGGGG
    TTTAGCAAAG ACTTCTCTGA GAATAAGCAG CCAGCCAACC CTCACCAGAA ACATGTACCT
    GTTCACAGTT TTTCCATGGA ACTCCTCAAG ACTGGAATGA GGTTGACGTA CATGCATGAT
    GACTCAGAGA GCCTTGCTGA TGATTTTACA ATCCGTTTGT CAGATGGGAA ACATAAGATA
    CTTAAAACCA TTTCAATAGA GGTCATCCCA GTTAATGATG AAAAACCAAT GCTGAGCAAG
    AAGGCTGAAA TTGCAATGAA TATGGGTGAA ACTCGTATTA TTTCCAGTGC TATTCTTTCA
    GCCATAGATG AAGACTCACC CAGGGAGAAG ATTTACTATG TATTTGAAAG GCTTCCCCAA
    AATGGGCAAC TTCAGCTTAA GATAGGGAGG GACTGGGTTC CTCTCTCCCC TGGCATGAAA
    TGCACTCAGG AGGAAGTGGA TCTGAACTTG CTGAGATACA CACACACCGG GGCAATGGAT
    TCCCAGAATC AAGATAGCTT CACCTTCTAC CTTTGGGATG GCAACAACAG GTCCCCTGCT
    CTTGACTGTC AAATCACCAT CAAGGACATG GAAAAAGGTG ATATTGTCAT CCTTACAAAA
    CCACTCGTGG TGTCTAAAGG TGATAGAGGT TTCTTAACAA CCACCACTCT CCTGGCTGTG
    GACGGAACAG ACAAGCCTGA GGAACTGCTC TATGTCATCA CCTCCCCTCC ACGATATGGC
    CAGATCGAAT ATGTTCACTA TCCTGGAGTT CCCATTACAA ACTTCAGCCA AATGGATATA
    GCGGGGCAGA CAGTTTGCTA TGTACACAAG AGCAAGGTGA CTGTCTCCAG TGACAGATTC
    AGATTCATCA TCAGCAATGG ACTGCGGACC GAGCACGGGG TGTTTGAGAT CACACTGGAG
    ACTGTGGACA GAGCCCTGCC TGTGGTAACC AGGAACAAGG GGTTGAGACT GGCCCAAGGG
    GCCGTGGGCC TGCTTTCCCC TGACCTCCTT CAGCTGACCG ACCCTGATAC ACCTGTGGAG
    AACCTCACCT TCCTCTTGGT TCAGCTTCCC CAGCATGGCC AGCTCTACCT GTGGGGGACA
    GGGCTACTTC AACACAATTT CACCCAGCAG GATGTGGACA GCAGGAATGT GGCCTATCGG
    CACTCAGGAG GGGACTCCCA GACTGACTGC TTTACTTTCA TGGCCACAGA CGGGACAAAC
    CAAGGCTTTA TTGTGAATGG GAGAGTATGG GAAGAACCTG TTTTATTCAC CATTCAGGTA
    GACCAATTGG ACAAAACAGC TCCTCGTATC ACACTCTTGC ATTCCCCTTC TCAAGTGGGG
    CTCCTGAAAA ATGGCTGCTA CGGGATTTAC ATCACTTCCC GCGTGTTGAA GGCATCAGAC
    CCTGACACTG AGGACGATCA GATCATCTTT AAAATTCTAC AAGGCCCAAA ACATGGACAT
    CTGGAGAACA CAACAACAGG TGAATTTATC CATGAGAAAT TTAGCCAAAA GGACTTAAAC
    AGTAAGACTA TTCTTTACAT CATAAACCCA TCTTTGGAAG TAAATTCAGA TACCGTGGAA
    TTTCAAATCA TGGACCCCAC AGGGAACTCG GCCACTCCTC AAATTTTGGA ACTGAAGTGG
    TCTCATATTG AATGGTCACA GACCGAATAT GAAGTCTGTG AGAATGTGGG TGTGTTGCCC
    TTGGAAATTA TCAGAAGGGG ATATTCCATG GACTCGGCCT TTGTGGGTAT AAAGGTCAAC
    CAAGTGTCAG CTGCAGATGG AAAAGATTTC ACCGTGATTC CATCTAAACT GATTCAGTTT
    GACCCAGGAA TGTCAACTAA GATGTGGAAT ATAGCAATTA CCTATGACGG ATTAGAGGAA
    GATGATGAGG TCTTTGAAGT AATTCTGAAC TCCCCTGTGA ATGCAGTTCT TGGCACAAAG
    ACAAAAGCTG CAGTGAAAAT TTTGGACTCA AAAGGAGGAC AATGCCATCC TTCATATTCC
    TCCAACCAAA GCAAGCACAG CACATGGGAG AAGGGCATTT GGCATCTGCT GCCCCCAGGG
    TCTTCCTCAT CCACCACTTC TGGTTCCTTT CATCTGGAAA GAAGACCTCT TCCATCTTCC
    ATGCAGCTAG CAGTCACCAG GGGAGACACC CTGCGGGGCT TTGATTCTAC AGATCTTTCT
    CAAAGGAAGC TTAGGACCCG TGGGAATGGC AAAACAGTTC GTCCATCCTC TGTTTATAGA
    AATGGAACAG ACATCATCTA TAATTATCAT GGGATAGTTT CCTTGAAACT AGAGGATGAC
    AGCTTCCCAA CTCACAAAAG GAAGGCCAAA GTATCCATCA TTAGTCAGCC ACAAAAGACA
    ATCAAAGTGG CAGAACTGCC TCAAGCAGAT AAGGTGGAAT CCACAACTGA CTCACACTTC
    CCCAGACAGG ACCAGTTGCC CTCATTTCCA AAGAACTGCA CTCTGGAATT AAAGGGACTC
    TTCCATTTTG AAGAAGGCAT CCAGAAGCTG TATCAGTGCA ATGGGATTGC CTGGAAAGCC
    TGGAGTCCCC AAACCAAGGA TGTGGAAGAA AAATCCTGTC CAGCCGGGTG GCACCAGCAC
    TCAGGCTACT GTCACATCTT GATCACAGAG CAGAAAGGCA CCTGGAATGC GGCTGCCCAA
    GCTTGCAGGG AACAATACCT GGGCAACCTT GTAACTGTAT TCTCCAGGCA GCACATGCGG
    TGGCTCTGGG ACATTGGTGA GAGAAAGTCC TTTTGGATAG GTTTGAACGA CCAAGTGCAT
    GCTGGCCACT GGGAGTGGAT CGGTGGTGAA CCTGTTGCCT TCACCAATGG GAGAAGAGGG
    CCCTCTCAAC GCTCCAAGCT TGGAAAGAGC TGTGTTTTGG TTCAAAGACA AGGGAAATGG
    CAAACAAAAG ATTGTAGGAG AGCCAAACCT CATAATTATG TGTGTTCCAG AAAACTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016816114.1
    CDS1938..8477
    Translation

    Target ORF information:

    RefSeq Version XM_016960625.1
    Organism Pan troglodytes(chimpanzee)
    Definition Pan troglodytes FRAS1 related extracellular matrix 1 (FREM1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_016960625.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGAACTCTC TGAGTTGGGG GGCTGCGAAT GCCGTGCTGC TGCTGCTCCT CCTGGCCTGG 
    GCCAGCCCCA CCTTCATCAG CATCAACCGT GGGGTGAGGG TGATGAAGGG CCACTCTGCC
    TTCCTGTCAG GAGATGACCT GAAGTTTGCC ATCCCTAAAG AGAAAGATGC CTGCAAAGTG
    GAAGTTGTGA TGAATGAGCC AATAACCCAG AGGGTTGGGA AACTCACTCC ACAGGTCTTT
    GACTGCCATT TCCTTCCCAA TGAAGTCAAG TATGTTCACA ATGGTTGTCC AATTCTTGAT
    GAAGACACAG TGAAGCTTAG ACTTTACAGA TTTACTGAAA GAGATACCTT CATAGAAACC
    TTTATCCTGT GGGTCTATCT CCTGGAACCA GACTGTAACA TCATCCATAT GAGTAACAAT
    GTGCTGGAGG TGCCTGAATT CAATGGCCTG TCCCAAGCGA TTGATAAAAA TCTGCTCAGA
    TTCGATTATG ATAGGATGGC TAGCCTGGAA TGTACGGTCA GCCTGGACAC TGCGAGAACT
    CGGCTGCCAG CCCATGGCCA GATGGTTCTC GGGGAGCCTC GACCAGAAGA ACCTCGTGGA
    GATCAGCCAC ACAGTTTTTT CCCAGAGTCC CAACTGAGAG CAAAACTGAA GTGTCCAGGT
    GGAAGCTGTA CCCCAGGATT AAAGAAAATA GGAAGCCTCA AAGTGAGCTG TGAGGAGTTC
    CTGCTGATGG GCCTTCGTTA TCAGCATCTG GATCCCCCTT CACCCAACAT TGATTATATC
    TCCATCCAAC TGGACCTCAC AGATACCAGG AGTAAAATTG TGTACAAGTC AGAGAGTGCG
    TGGCTGCCTG TCTATATCAG AGCTGGAATT CCGAATCAGA TTCCAAAGGC TGCATTCATG
    GCCATGTTTA TTCTGGAAGT GGATCAGTTC ATCCTGACCT CCTTGACTAC ATCAGTTCTG
    GACTGTGAAG AAGATGAGAC CCCTAAACCC TTGCTGGTGT TCAACATTAC TAAAGCCCCG
    CTCCAGGGCT ATGTGACTCA CCTGTTGGAT CACACCAGAC CAATCTCCTC ATTCACCTGG
    AAAGATCTCA GTGACATGCA GATCGCCTAT CAGCCACCAA ACAGCAGCCA TTCTGAGAGG
    AGACATGATG AGGTAGAATT GGAGGTATAC GACTTCTTCT TTGAAAGGAG TGCACCTATG
    ACAGTCCACA TCTCCATCAG AACAGCAGAT ACAAATGCCC CCCGTGTATC CTGGAATACA
    GGTCTGAATC TCCTTGAGGG GCAGTCTCGA GCCATCACTT GGGAACAGTT TCAGGTTGTC
    GACAATGACG ACATCGGTGC TGTCCGGCTA GTCACCGTTG GTGGCCTGCA GCATGGATGG
    CTGACTTTAA GAGGGGGGAA AGGATTTCTC TTCACCGTGG CTGACCTCCA GGCTGGAGTT
    GTTCGCTATC ATCATGATGA CAGCGACTCC ACCAAAGACT TCGTGGTCTT CCGGATATTT
    GATGGCCATC ACAGCATCCG TCACAAATTC CCCATCAACG TCTTGCCCAA AGATGATAGT
    CCCCCGTTCC TCATAACCAA TGTTGTCATT GAACTGGAGG AGGGGCAGAC CATCCTGATC
    CAGGGATCCA TGCTGCGAGC TTCAGATGTG GATGCCAGTG ATGACTACAT CTTCTTCAAT
    ATCACAAAGC CTCCACAGGC TGGGGAGATC ATGAAGAAGC CAGGGCCAGG ACTGATAGGC
    TATCCTGTCC CTGGCTTCCT TCAGAGGGAT TTGTTTAATG GAATCATTTA TTATCGTCAT
    TTTGGTGGAG AAATCTTTGA AGATTCTTTT CAATTTGTCC TGTGGGACAG CCATGAACCT
    CCAAATCTTT CAGTGCCACA GGTGGCAACA ATCCATGTAA CTCCAGTGGA TGACCAGCTT
    CCAAAAGAGG CTCCTGGAGT TTCTCGGCAT TTGGTTGTCA AGGAAACTGA GGTGGCCTAT
    ATAACTAAGA AACAGCTACA TTTTATAGAT TCAGAATCAT ATGACAGGGA GCTGGTCTAC
    ACGATAACTA CTCCTCCATT TTTCTCCTTC AGCCACAGAC ACTTGGATGC TGGGAAATTA
    TTTATGGTGG ACAGCATACC AAAAGTAGTT AAGAATCCTA CGGCCCTGGA GCTGAGGTCA
    TTCACTCAGC ATGCTGTGAA CTATATGAAA GTGGCCTACA TGCCCCCCAT GCAAGACATT
    GGTCCCCATT GCAGAGATGT CCAGTTCACA TTTTCTGTCA GTAACCAACA TGGCGGCACT
    TTGCATGGGA TCTGCTTTAA CATTACAATC CTCCCAGTGG ACAATCAAGT TCCTGAGGCG
    TTCACCAACC CTCTGAAAGT GACTGAGGGA GGTCAAAGCA TCATCAGCAC AGAGCACATT
    CTGATTTCTG ATGCAGATAC CAAGCTGGAC AATATTGACC TCTCCCTGCG GGAATTGCCT
    CTGCACGGAA GGGTGGAGCT GAATGGATTT CCTCTAAATT CAGGGGGCAC ATTTTCTTGG
    GGCGATCTCC ATACCTTAAA AGTTAGGTAT CAACATGATG GAACTGAAGT TCTTCAGGAT
    GACCTACTCT TGGAGGTCAC CGATGGCACA AATTCAGCAG AATTTGTTCT ACATGTTGAG
    GTATTCCCTG TCAACGATGA GCCACCAGTC TTAAAGGCTG ACCTCATGCC TGTCATGAAT
    TGCTCAGAGG GAGGAGAGGT GGTCATCACC TCTGAATACA TTTTTGCTAC TGATGTGGAC
    AGCGATAACT TGAAGTTGAT GTTTGTGATT GCTCGCGAAC CTCAGCATGG GGTGGTGAGG
    AGAGCTGGAG TCACAGTGGA TCAGTTCTCT CAGAGAGATG TTATCTCAGA GGCCGTGACA
    TACAAACACA CAGGTGGCGA GATTGGCATG ATGCCCTGTT TTGACACCAT TACATTGGTG
    GTTTCGGATG GAGAGGCTGG CCCTTTTGTG AATGGCTGTT GCTACAATGG ACCCAATCCA
    TCTGTTCCGC TTCATGCGTC CTTTCCAGTG TATGATCTCA ACATCACGGT ATACCCAGTA
    GACAACCAGC CACCTTCCGT TGCAATAGGT CCCATGTTTG TTGTAGATGA GGGCTGCTCA
    ACAGCCCTTA CTGTTAACCA TTTGTCCGCC ACTGACCCTG ACACTGCAGC AGATGACTTG
    GAATTTGTTT TGGTTTCTCC TCCTCAGTTT GGCTACCTCG AAAATATACT CCCTTCTGTG
    GGTTTTGAAA AAAGCAATAT TGGCATAAGT ATAGATTCAT TTCAGTGGAA AGACATGAAC
    GCTTTTCACA TTAACTATGT GCAGTCCAGG CATCTGAGGA TAGAACCAAC TGCCGACCAG
    TTCACGGTGT ACGTCACAGA TGGGAAGCAA CACTCCTTGG AGATACCATT TTCTATTATA
    ATCAACCCCA CAAATGATGA AGCTCCTGAC TTTGTAGTGC AGAATATTAC TGTGTGCGAG
    GGTCAGATGA AAGAGCTGGA CTCTTCCATC ATCAGCGCTG TGGACCTGGA CATTCCCCAG
    GATGCCCTGC TGTTCAGCAT CACTCAAAAG CCACGCCATG GCCTCCTCAT CGATAGGGGG
    TTTAGCAAAG ACTTCTCTGA GAATAAGCAG CCAGCCAACC CTCACCAGAA ACATGTACCT
    GTTCACAGTT TTTCCATGGA ACTCCTCAAG ACTGGAATGA GGTTGACGTA CATGCATGAT
    GACTCAGAGA GCCTTGCTGA TGATTTTACA ATCCGTTTGT CAGATGGGAA ACATAAGATA
    CTTAAAACCA TTTCAATAGA GGTCATCCCA GTTAATGATG AAAAACCAAT GCTGAGCAAG
    AAGGCTGAAA TTGCAATGAA TATGGGTGAA ACTCGTATTA TTTCCAGTGC TATTCTTTCA
    GCCATAGATG AAGACTCACC CAGGGAGAAG ATTTACTATG TATTTGAAAG GCTTCCCCAA
    AATGGGCAAC TTCAGCTTAA GATAGGGAGG GACTGGGTTC CTCTCTCCCC TGGCATGAAA
    TGCACTCAGG AGGAAGTGGA TCTGAACTTG CTGAGATACA CACACACCGG GGCAATGGAT
    TCCCAGAATC AAGATAGCTT CACCTTCTAC CTTTGGGATG GCAACAACAG GTCCCCTGCT
    CTTGACTGTC AAATCACCAT CAAGGACATG GAAAAAGGTG ATATTGTCAT CCTTACAAAA
    CCACTCGTGG TGTCTAAAGG TGATAGAGGT TTCTTAACAA CCACCACTCT CCTGGCTGTG
    GACGGAACAG ACAAGCCTGA GGAACTGCTC TATGTCATCA CCTCCCCTCC ACGATATGGC
    CAGATCGAAT ATGTTCACTA TCCTGGAGTT CCCATTACAA ACTTCAGCCA AATGGATATA
    GCGGGGCAGA CAGTTTGCTA TGTACACAAG AGCAAGGTGA CTGTCTCCAG TGACAGATTC
    AGATTCATCA TCAGCAATGG ACTGCGGACC GAGCACGGGG TGTTTGAGAT CACACTGGAG
    ACTGTGGACA GAGCCCTGCC TGTGGTAACC AGGAACAAGG GGTTGAGACT GGCCCAAGGG
    GCCGTGGGCC TGCTTTCCCC TGACCTCCTT CAGCTGACCG ACCCTGATAC ACCTGTGGAG
    AACCTCACCT TCCTCTTGGT TCAGCTTCCC CAGCATGGCC AGCTCTACCT GTGGGGGACA
    GGGCTACTTC AACACAATTT CACCCAGCAG GATGTGGACA GCAGGAATGT GGCCTATCGG
    CACTCAGGAG GGGACTCCCA GACTGACTGC TTTACTTTCA TGGCCACAGA CGGGACAAAC
    CAAGGCTTTA TTGTGAATGG GAGAGTATGG GAAGAACCTG TTTTATTCAC CATTCAGGTA
    GACCAATTGG ACAAAACAGC TCCTCGTATC ACACTCTTGC ATTCCCCTTC TCAAGTGGGG
    CTCCTGAAAA ATGGCTGCTA CGGGATTTAC ATCACTTCCC GCGTGTTGAA GGCATCAGAC
    CCTGACACTG AGGACGATCA GATCATCTTT AAAATTCTAC AAGGCCCAAA ACATGGACAT
    CTGGAGAACA CAACAACAGG TGAATTTATC CATGAGAAAT TTAGCCAAAA GGACTTAAAC
    AGTAAGACTA TTCTTTACAT CATAAACCCA TCTTTGGAAG TAAATTCAGA TACCGTGGAA
    TTTCAAATCA TGGACCCCAC AGGGAACTCG GCCACTCCTC AAATTTTGGA ACTGAAGTGG
    TCTCATATTG AATGGTCACA GACCGAATAT GAAGTCTGTG AGAATGTGGG TGTGTTGCCC
    TTGGAAATTA TCAGAAGGGG ATATTCCATG GACTCGGCCT TTGTGGGTAT AAAGGTCAAC
    CAAGTGTCAG CTGCAGATGG AAAAGATTTC ACCGTGATTC CATCTAAACT GATTCAGTTT
    GACCCAGGAA TGTCAACTAA GATGTGGAAT ATAGCAATTA CCTATGACGG ATTAGAGGAA
    GATGATGAGG TCTTTGAAGT AATTCTGAAC TCCCCTGTGA ATGCAGTTCT TGGCACAAAG
    ACAAAAGCTG CAGTGAAAAT TTTGGACTCA AAAGGAGGAC AATGCCATCC TTCATATTCC
    TCCAACCAAA GCAAGCACAG CACATGGGAG AAGGGCATTT GGCATCTGCT GCCCCCAGGG
    TCTTCCTCAT CCACCACTTC TGGTTCCTTT CATCTGGAAA GAAGACCTCT TCCATCTTCC
    ATGCAGCTAG CAGTCACCAG GGGAGACACC CTGCGGGGCT TTGATTCTAC AGATCTTTCT
    CAAAGGAAGC TTAGGACCCG TGGGAATGGC AAAACAGTTC GTCCATCCTC TGTTTATAGA
    AATGGAACAG ACATCATCTA TAATTATCAT GGGATAGTTT CCTTGAAACT AGAGGATGAC
    AGCTTCCCAA CTCACAAAAG GAAGGCCAAA GTATCCATCA TTAGTCAGCC ACAAAAGACA
    ATCAAAGTGG CAGAACTGCC TCAAGCAGAT AAGGTGGAAT CCACAACTGA CTCACACTTC
    CCCAGACAGG ACCAGTTGCC CTCATTTCCA AAGAACTGCA CTCTGGAATT AAAGGGACTC
    TTCCATTTTG AAGAAGGCAT CCAGAAGCTG TATCAGTGCA ATGGGATTGC CTGGAAAGCC
    TGGAGTCCCC AAACCAAGGA TGTGGAAGAA AAATCCTGTC CAGCCGGGTG GCACCAGCAC
    TCAGGCTACT GTCACATCTT GATCACAGAG CAGAAAGGCA CCTGGAATGC GGCTGCCCAA
    GCTTGCAGGG AACAATACCT GGGCAACCTT GTAACTGTAT TCTCCAGGCA GCACATGCGG
    TGGCTCTGGG ACATTGGTGA GAGAAAGTCC TTTTGGATAG GTTTGAACGA CCAAGTGCAT
    GCTGGCCACT GGGAGTGGAT CGGTGGTGAA CCTGTTGCCT TCACCAATGG GAGAAGAGGG
    CCCTCTCAAC GCTCCAAGCT TGGAAAGAGC TGTGTTTTGG TTCAAAGACA AGGGAAATGG
    CAAACAAAAG ATTGTAGGAG AGCCAAACCT CATAATTATG TGTGTTCCAG AAAACTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OPc88756
    Clone ID Related Accession (Same CDS sequence) XM_009456318.2 , XM_016960627.1 , XM_016960625.1 , XM_016960626.1
    Accession Version XM_016960626.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6540bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-01
    Organism Pan troglodytes(chimpanzee)
    Product FRAS1-related extracellular matrix protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006476.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGAACTCTC TGAGTTGGGG GGCTGCGAAT GCCGTGCTGC TGCTGCTCCT CCTGGCCTGG 
    GCCAGCCCCA CCTTCATCAG CATCAACCGT GGGGTGAGGG TGATGAAGGG CCACTCTGCC
    TTCCTGTCAG GAGATGACCT GAAGTTTGCC ATCCCTAAAG AGAAAGATGC CTGCAAAGTG
    GAAGTTGTGA TGAATGAGCC AATAACCCAG AGGGTTGGGA AACTCACTCC ACAGGTCTTT
    GACTGCCATT TCCTTCCCAA TGAAGTCAAG TATGTTCACA ATGGTTGTCC AATTCTTGAT
    GAAGACACAG TGAAGCTTAG ACTTTACAGA TTTACTGAAA GAGATACCTT CATAGAAACC
    TTTATCCTGT GGGTCTATCT CCTGGAACCA GACTGTAACA TCATCCATAT GAGTAACAAT
    GTGCTGGAGG TGCCTGAATT CAATGGCCTG TCCCAAGCGA TTGATAAAAA TCTGCTCAGA
    TTCGATTATG ATAGGATGGC TAGCCTGGAA TGTACGGTCA GCCTGGACAC TGCGAGAACT
    CGGCTGCCAG CCCATGGCCA GATGGTTCTC GGGGAGCCTC GACCAGAAGA ACCTCGTGGA
    GATCAGCCAC ACAGTTTTTT CCCAGAGTCC CAACTGAGAG CAAAACTGAA GTGTCCAGGT
    GGAAGCTGTA CCCCAGGATT AAAGAAAATA GGAAGCCTCA AAGTGAGCTG TGAGGAGTTC
    CTGCTGATGG GCCTTCGTTA TCAGCATCTG GATCCCCCTT CACCCAACAT TGATTATATC
    TCCATCCAAC TGGACCTCAC AGATACCAGG AGTAAAATTG TGTACAAGTC AGAGAGTGCG
    TGGCTGCCTG TCTATATCAG AGCTGGAATT CCGAATCAGA TTCCAAAGGC TGCATTCATG
    GCCATGTTTA TTCTGGAAGT GGATCAGTTC ATCCTGACCT CCTTGACTAC ATCAGTTCTG
    GACTGTGAAG AAGATGAGAC CCCTAAACCC TTGCTGGTGT TCAACATTAC TAAAGCCCCG
    CTCCAGGGCT ATGTGACTCA CCTGTTGGAT CACACCAGAC CAATCTCCTC ATTCACCTGG
    AAAGATCTCA GTGACATGCA GATCGCCTAT CAGCCACCAA ACAGCAGCCA TTCTGAGAGG
    AGACATGATG AGGTAGAATT GGAGGTATAC GACTTCTTCT TTGAAAGGAG TGCACCTATG
    ACAGTCCACA TCTCCATCAG AACAGCAGAT ACAAATGCCC CCCGTGTATC CTGGAATACA
    GGTCTGAATC TCCTTGAGGG GCAGTCTCGA GCCATCACTT GGGAACAGTT TCAGGTTGTC
    GACAATGACG ACATCGGTGC TGTCCGGCTA GTCACCGTTG GTGGCCTGCA GCATGGATGG
    CTGACTTTAA GAGGGGGGAA AGGATTTCTC TTCACCGTGG CTGACCTCCA GGCTGGAGTT
    GTTCGCTATC ATCATGATGA CAGCGACTCC ACCAAAGACT TCGTGGTCTT CCGGATATTT
    GATGGCCATC ACAGCATCCG TCACAAATTC CCCATCAACG TCTTGCCCAA AGATGATAGT
    CCCCCGTTCC TCATAACCAA TGTTGTCATT GAACTGGAGG AGGGGCAGAC CATCCTGATC
    CAGGGATCCA TGCTGCGAGC TTCAGATGTG GATGCCAGTG ATGACTACAT CTTCTTCAAT
    ATCACAAAGC CTCCACAGGC TGGGGAGATC ATGAAGAAGC CAGGGCCAGG ACTGATAGGC
    TATCCTGTCC CTGGCTTCCT TCAGAGGGAT TTGTTTAATG GAATCATTTA TTATCGTCAT
    TTTGGTGGAG AAATCTTTGA AGATTCTTTT CAATTTGTCC TGTGGGACAG CCATGAACCT
    CCAAATCTTT CAGTGCCACA GGTGGCAACA ATCCATGTAA CTCCAGTGGA TGACCAGCTT
    CCAAAAGAGG CTCCTGGAGT TTCTCGGCAT TTGGTTGTCA AGGAAACTGA GGTGGCCTAT
    ATAACTAAGA AACAGCTACA TTTTATAGAT TCAGAATCAT ATGACAGGGA GCTGGTCTAC
    ACGATAACTA CTCCTCCATT TTTCTCCTTC AGCCACAGAC ACTTGGATGC TGGGAAATTA
    TTTATGGTGG ACAGCATACC AAAAGTAGTT AAGAATCCTA CGGCCCTGGA GCTGAGGTCA
    TTCACTCAGC ATGCTGTGAA CTATATGAAA GTGGCCTACA TGCCCCCCAT GCAAGACATT
    GGTCCCCATT GCAGAGATGT CCAGTTCACA TTTTCTGTCA GTAACCAACA TGGCGGCACT
    TTGCATGGGA TCTGCTTTAA CATTACAATC CTCCCAGTGG ACAATCAAGT TCCTGAGGCG
    TTCACCAACC CTCTGAAAGT GACTGAGGGA GGTCAAAGCA TCATCAGCAC AGAGCACATT
    CTGATTTCTG ATGCAGATAC CAAGCTGGAC AATATTGACC TCTCCCTGCG GGAATTGCCT
    CTGCACGGAA GGGTGGAGCT GAATGGATTT CCTCTAAATT CAGGGGGCAC ATTTTCTTGG
    GGCGATCTCC ATACCTTAAA AGTTAGGTAT CAACATGATG GAACTGAAGT TCTTCAGGAT
    GACCTACTCT TGGAGGTCAC CGATGGCACA AATTCAGCAG AATTTGTTCT ACATGTTGAG
    GTATTCCCTG TCAACGATGA GCCACCAGTC TTAAAGGCTG ACCTCATGCC TGTCATGAAT
    TGCTCAGAGG GAGGAGAGGT GGTCATCACC TCTGAATACA TTTTTGCTAC TGATGTGGAC
    AGCGATAACT TGAAGTTGAT GTTTGTGATT GCTCGCGAAC CTCAGCATGG GGTGGTGAGG
    AGAGCTGGAG TCACAGTGGA TCAGTTCTCT CAGAGAGATG TTATCTCAGA GGCCGTGACA
    TACAAACACA CAGGTGGCGA GATTGGCATG ATGCCCTGTT TTGACACCAT TACATTGGTG
    GTTTCGGATG GAGAGGCTGG CCCTTTTGTG AATGGCTGTT GCTACAATGG ACCCAATCCA
    TCTGTTCCGC TTCATGCGTC CTTTCCAGTG TATGATCTCA ACATCACGGT ATACCCAGTA
    GACAACCAGC CACCTTCCGT TGCAATAGGT CCCATGTTTG TTGTAGATGA GGGCTGCTCA
    ACAGCCCTTA CTGTTAACCA TTTGTCCGCC ACTGACCCTG ACACTGCAGC AGATGACTTG
    GAATTTGTTT TGGTTTCTCC TCCTCAGTTT GGCTACCTCG AAAATATACT CCCTTCTGTG
    GGTTTTGAAA AAAGCAATAT TGGCATAAGT ATAGATTCAT TTCAGTGGAA AGACATGAAC
    GCTTTTCACA TTAACTATGT GCAGTCCAGG CATCTGAGGA TAGAACCAAC TGCCGACCAG
    TTCACGGTGT ACGTCACAGA TGGGAAGCAA CACTCCTTGG AGATACCATT TTCTATTATA
    ATCAACCCCA CAAATGATGA AGCTCCTGAC TTTGTAGTGC AGAATATTAC TGTGTGCGAG
    GGTCAGATGA AAGAGCTGGA CTCTTCCATC ATCAGCGCTG TGGACCTGGA CATTCCCCAG
    GATGCCCTGC TGTTCAGCAT CACTCAAAAG CCACGCCATG GCCTCCTCAT CGATAGGGGG
    TTTAGCAAAG ACTTCTCTGA GAATAAGCAG CCAGCCAACC CTCACCAGAA ACATGTACCT
    GTTCACAGTT TTTCCATGGA ACTCCTCAAG ACTGGAATGA GGTTGACGTA CATGCATGAT
    GACTCAGAGA GCCTTGCTGA TGATTTTACA ATCCGTTTGT CAGATGGGAA ACATAAGATA
    CTTAAAACCA TTTCAATAGA GGTCATCCCA GTTAATGATG AAAAACCAAT GCTGAGCAAG
    AAGGCTGAAA TTGCAATGAA TATGGGTGAA ACTCGTATTA TTTCCAGTGC TATTCTTTCA
    GCCATAGATG AAGACTCACC CAGGGAGAAG ATTTACTATG TATTTGAAAG GCTTCCCCAA
    AATGGGCAAC TTCAGCTTAA GATAGGGAGG GACTGGGTTC CTCTCTCCCC TGGCATGAAA
    TGCACTCAGG AGGAAGTGGA TCTGAACTTG CTGAGATACA CACACACCGG GGCAATGGAT
    TCCCAGAATC AAGATAGCTT CACCTTCTAC CTTTGGGATG GCAACAACAG GTCCCCTGCT
    CTTGACTGTC AAATCACCAT CAAGGACATG GAAAAAGGTG ATATTGTCAT CCTTACAAAA
    CCACTCGTGG TGTCTAAAGG TGATAGAGGT TTCTTAACAA CCACCACTCT CCTGGCTGTG
    GACGGAACAG ACAAGCCTGA GGAACTGCTC TATGTCATCA CCTCCCCTCC ACGATATGGC
    CAGATCGAAT ATGTTCACTA TCCTGGAGTT CCCATTACAA ACTTCAGCCA AATGGATATA
    GCGGGGCAGA CAGTTTGCTA TGTACACAAG AGCAAGGTGA CTGTCTCCAG TGACAGATTC
    AGATTCATCA TCAGCAATGG ACTGCGGACC GAGCACGGGG TGTTTGAGAT CACACTGGAG
    ACTGTGGACA GAGCCCTGCC TGTGGTAACC AGGAACAAGG GGTTGAGACT GGCCCAAGGG
    GCCGTGGGCC TGCTTTCCCC TGACCTCCTT CAGCTGACCG ACCCTGATAC ACCTGTGGAG
    AACCTCACCT TCCTCTTGGT TCAGCTTCCC CAGCATGGCC AGCTCTACCT GTGGGGGACA
    GGGCTACTTC AACACAATTT CACCCAGCAG GATGTGGACA GCAGGAATGT GGCCTATCGG
    CACTCAGGAG GGGACTCCCA GACTGACTGC TTTACTTTCA TGGCCACAGA CGGGACAAAC
    CAAGGCTTTA TTGTGAATGG GAGAGTATGG GAAGAACCTG TTTTATTCAC CATTCAGGTA
    GACCAATTGG ACAAAACAGC TCCTCGTATC ACACTCTTGC ATTCCCCTTC TCAAGTGGGG
    CTCCTGAAAA ATGGCTGCTA CGGGATTTAC ATCACTTCCC GCGTGTTGAA GGCATCAGAC
    CCTGACACTG AGGACGATCA GATCATCTTT AAAATTCTAC AAGGCCCAAA ACATGGACAT
    CTGGAGAACA CAACAACAGG TGAATTTATC CATGAGAAAT TTAGCCAAAA GGACTTAAAC
    AGTAAGACTA TTCTTTACAT CATAAACCCA TCTTTGGAAG TAAATTCAGA TACCGTGGAA
    TTTCAAATCA TGGACCCCAC AGGGAACTCG GCCACTCCTC AAATTTTGGA ACTGAAGTGG
    TCTCATATTG AATGGTCACA GACCGAATAT GAAGTCTGTG AGAATGTGGG TGTGTTGCCC
    TTGGAAATTA TCAGAAGGGG ATATTCCATG GACTCGGCCT TTGTGGGTAT AAAGGTCAAC
    CAAGTGTCAG CTGCAGATGG AAAAGATTTC ACCGTGATTC CATCTAAACT GATTCAGTTT
    GACCCAGGAA TGTCAACTAA GATGTGGAAT ATAGCAATTA CCTATGACGG ATTAGAGGAA
    GATGATGAGG TCTTTGAAGT AATTCTGAAC TCCCCTGTGA ATGCAGTTCT TGGCACAAAG
    ACAAAAGCTG CAGTGAAAAT TTTGGACTCA AAAGGAGGAC AATGCCATCC TTCATATTCC
    TCCAACCAAA GCAAGCACAG CACATGGGAG AAGGGCATTT GGCATCTGCT GCCCCCAGGG
    TCTTCCTCAT CCACCACTTC TGGTTCCTTT CATCTGGAAA GAAGACCTCT TCCATCTTCC
    ATGCAGCTAG CAGTCACCAG GGGAGACACC CTGCGGGGCT TTGATTCTAC AGATCTTTCT
    CAAAGGAAGC TTAGGACCCG TGGGAATGGC AAAACAGTTC GTCCATCCTC TGTTTATAGA
    AATGGAACAG ACATCATCTA TAATTATCAT GGGATAGTTT CCTTGAAACT AGAGGATGAC
    AGCTTCCCAA CTCACAAAAG GAAGGCCAAA GTATCCATCA TTAGTCAGCC ACAAAAGACA
    ATCAAAGTGG CAGAACTGCC TCAAGCAGAT AAGGTGGAAT CCACAACTGA CTCACACTTC
    CCCAGACAGG ACCAGTTGCC CTCATTTCCA AAGAACTGCA CTCTGGAATT AAAGGGACTC
    TTCCATTTTG AAGAAGGCAT CCAGAAGCTG TATCAGTGCA ATGGGATTGC CTGGAAAGCC
    TGGAGTCCCC AAACCAAGGA TGTGGAAGAA AAATCCTGTC CAGCCGGGTG GCACCAGCAC
    TCAGGCTACT GTCACATCTT GATCACAGAG CAGAAAGGCA CCTGGAATGC GGCTGCCCAA
    GCTTGCAGGG AACAATACCT GGGCAACCTT GTAACTGTAT TCTCCAGGCA GCACATGCGG
    TGGCTCTGGG ACATTGGTGA GAGAAAGTCC TTTTGGATAG GTTTGAACGA CCAAGTGCAT
    GCTGGCCACT GGGAGTGGAT CGGTGGTGAA CCTGTTGCCT TCACCAATGG GAGAAGAGGG
    CCCTCTCAAC GCTCCAAGCT TGGAAAGAGC TGTGTTTTGG TTCAAAGACA AGGGAAATGG
    CAAACAAAAG ATTGTAGGAG AGCCAAACCT CATAATTATG TGTGTTCCAG AAAACTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016816115.1
    CDS2013..8552
    Translation

    Target ORF information:

    RefSeq Version XM_016960626.1
    Organism Pan troglodytes(chimpanzee)
    Definition Pan troglodytes FRAS1 related extracellular matrix 1 (FREM1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_016960626.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGAACTCTC TGAGTTGGGG GGCTGCGAAT GCCGTGCTGC TGCTGCTCCT CCTGGCCTGG 
    GCCAGCCCCA CCTTCATCAG CATCAACCGT GGGGTGAGGG TGATGAAGGG CCACTCTGCC
    TTCCTGTCAG GAGATGACCT GAAGTTTGCC ATCCCTAAAG AGAAAGATGC CTGCAAAGTG
    GAAGTTGTGA TGAATGAGCC AATAACCCAG AGGGTTGGGA AACTCACTCC ACAGGTCTTT
    GACTGCCATT TCCTTCCCAA TGAAGTCAAG TATGTTCACA ATGGTTGTCC AATTCTTGAT
    GAAGACACAG TGAAGCTTAG ACTTTACAGA TTTACTGAAA GAGATACCTT CATAGAAACC
    TTTATCCTGT GGGTCTATCT CCTGGAACCA GACTGTAACA TCATCCATAT GAGTAACAAT
    GTGCTGGAGG TGCCTGAATT CAATGGCCTG TCCCAAGCGA TTGATAAAAA TCTGCTCAGA
    TTCGATTATG ATAGGATGGC TAGCCTGGAA TGTACGGTCA GCCTGGACAC TGCGAGAACT
    CGGCTGCCAG CCCATGGCCA GATGGTTCTC GGGGAGCCTC GACCAGAAGA ACCTCGTGGA
    GATCAGCCAC ACAGTTTTTT CCCAGAGTCC CAACTGAGAG CAAAACTGAA GTGTCCAGGT
    GGAAGCTGTA CCCCAGGATT AAAGAAAATA GGAAGCCTCA AAGTGAGCTG TGAGGAGTTC
    CTGCTGATGG GCCTTCGTTA TCAGCATCTG GATCCCCCTT CACCCAACAT TGATTATATC
    TCCATCCAAC TGGACCTCAC AGATACCAGG AGTAAAATTG TGTACAAGTC AGAGAGTGCG
    TGGCTGCCTG TCTATATCAG AGCTGGAATT CCGAATCAGA TTCCAAAGGC TGCATTCATG
    GCCATGTTTA TTCTGGAAGT GGATCAGTTC ATCCTGACCT CCTTGACTAC ATCAGTTCTG
    GACTGTGAAG AAGATGAGAC CCCTAAACCC TTGCTGGTGT TCAACATTAC TAAAGCCCCG
    CTCCAGGGCT ATGTGACTCA CCTGTTGGAT CACACCAGAC CAATCTCCTC ATTCACCTGG
    AAAGATCTCA GTGACATGCA GATCGCCTAT CAGCCACCAA ACAGCAGCCA TTCTGAGAGG
    AGACATGATG AGGTAGAATT GGAGGTATAC GACTTCTTCT TTGAAAGGAG TGCACCTATG
    ACAGTCCACA TCTCCATCAG AACAGCAGAT ACAAATGCCC CCCGTGTATC CTGGAATACA
    GGTCTGAATC TCCTTGAGGG GCAGTCTCGA GCCATCACTT GGGAACAGTT TCAGGTTGTC
    GACAATGACG ACATCGGTGC TGTCCGGCTA GTCACCGTTG GTGGCCTGCA GCATGGATGG
    CTGACTTTAA GAGGGGGGAA AGGATTTCTC TTCACCGTGG CTGACCTCCA GGCTGGAGTT
    GTTCGCTATC ATCATGATGA CAGCGACTCC ACCAAAGACT TCGTGGTCTT CCGGATATTT
    GATGGCCATC ACAGCATCCG TCACAAATTC CCCATCAACG TCTTGCCCAA AGATGATAGT
    CCCCCGTTCC TCATAACCAA TGTTGTCATT GAACTGGAGG AGGGGCAGAC CATCCTGATC
    CAGGGATCCA TGCTGCGAGC TTCAGATGTG GATGCCAGTG ATGACTACAT CTTCTTCAAT
    ATCACAAAGC CTCCACAGGC TGGGGAGATC ATGAAGAAGC CAGGGCCAGG ACTGATAGGC
    TATCCTGTCC CTGGCTTCCT TCAGAGGGAT TTGTTTAATG GAATCATTTA TTATCGTCAT
    TTTGGTGGAG AAATCTTTGA AGATTCTTTT CAATTTGTCC TGTGGGACAG CCATGAACCT
    CCAAATCTTT CAGTGCCACA GGTGGCAACA ATCCATGTAA CTCCAGTGGA TGACCAGCTT
    CCAAAAGAGG CTCCTGGAGT TTCTCGGCAT TTGGTTGTCA AGGAAACTGA GGTGGCCTAT
    ATAACTAAGA AACAGCTACA TTTTATAGAT TCAGAATCAT ATGACAGGGA GCTGGTCTAC
    ACGATAACTA CTCCTCCATT TTTCTCCTTC AGCCACAGAC ACTTGGATGC TGGGAAATTA
    TTTATGGTGG ACAGCATACC AAAAGTAGTT AAGAATCCTA CGGCCCTGGA GCTGAGGTCA
    TTCACTCAGC ATGCTGTGAA CTATATGAAA GTGGCCTACA TGCCCCCCAT GCAAGACATT
    GGTCCCCATT GCAGAGATGT CCAGTTCACA TTTTCTGTCA GTAACCAACA TGGCGGCACT
    TTGCATGGGA TCTGCTTTAA CATTACAATC CTCCCAGTGG ACAATCAAGT TCCTGAGGCG
    TTCACCAACC CTCTGAAAGT GACTGAGGGA GGTCAAAGCA TCATCAGCAC AGAGCACATT
    CTGATTTCTG ATGCAGATAC CAAGCTGGAC AATATTGACC TCTCCCTGCG GGAATTGCCT
    CTGCACGGAA GGGTGGAGCT GAATGGATTT CCTCTAAATT CAGGGGGCAC ATTTTCTTGG
    GGCGATCTCC ATACCTTAAA AGTTAGGTAT CAACATGATG GAACTGAAGT TCTTCAGGAT
    GACCTACTCT TGGAGGTCAC CGATGGCACA AATTCAGCAG AATTTGTTCT ACATGTTGAG
    GTATTCCCTG TCAACGATGA GCCACCAGTC TTAAAGGCTG ACCTCATGCC TGTCATGAAT
    TGCTCAGAGG GAGGAGAGGT GGTCATCACC TCTGAATACA TTTTTGCTAC TGATGTGGAC
    AGCGATAACT TGAAGTTGAT GTTTGTGATT GCTCGCGAAC CTCAGCATGG GGTGGTGAGG
    AGAGCTGGAG TCACAGTGGA TCAGTTCTCT CAGAGAGATG TTATCTCAGA GGCCGTGACA
    TACAAACACA CAGGTGGCGA GATTGGCATG ATGCCCTGTT TTGACACCAT TACATTGGTG
    GTTTCGGATG GAGAGGCTGG CCCTTTTGTG AATGGCTGTT GCTACAATGG ACCCAATCCA
    TCTGTTCCGC TTCATGCGTC CTTTCCAGTG TATGATCTCA ACATCACGGT ATACCCAGTA
    GACAACCAGC CACCTTCCGT TGCAATAGGT CCCATGTTTG TTGTAGATGA GGGCTGCTCA
    ACAGCCCTTA CTGTTAACCA TTTGTCCGCC ACTGACCCTG ACACTGCAGC AGATGACTTG
    GAATTTGTTT TGGTTTCTCC TCCTCAGTTT GGCTACCTCG AAAATATACT CCCTTCTGTG
    GGTTTTGAAA AAAGCAATAT TGGCATAAGT ATAGATTCAT TTCAGTGGAA AGACATGAAC
    GCTTTTCACA TTAACTATGT GCAGTCCAGG CATCTGAGGA TAGAACCAAC TGCCGACCAG
    TTCACGGTGT ACGTCACAGA TGGGAAGCAA CACTCCTTGG AGATACCATT TTCTATTATA
    ATCAACCCCA CAAATGATGA AGCTCCTGAC TTTGTAGTGC AGAATATTAC TGTGTGCGAG
    GGTCAGATGA AAGAGCTGGA CTCTTCCATC ATCAGCGCTG TGGACCTGGA CATTCCCCAG
    GATGCCCTGC TGTTCAGCAT CACTCAAAAG CCACGCCATG GCCTCCTCAT CGATAGGGGG
    TTTAGCAAAG ACTTCTCTGA GAATAAGCAG CCAGCCAACC CTCACCAGAA ACATGTACCT
    GTTCACAGTT TTTCCATGGA ACTCCTCAAG ACTGGAATGA GGTTGACGTA CATGCATGAT
    GACTCAGAGA GCCTTGCTGA TGATTTTACA ATCCGTTTGT CAGATGGGAA ACATAAGATA
    CTTAAAACCA TTTCAATAGA GGTCATCCCA GTTAATGATG AAAAACCAAT GCTGAGCAAG
    AAGGCTGAAA TTGCAATGAA TATGGGTGAA ACTCGTATTA TTTCCAGTGC TATTCTTTCA
    GCCATAGATG AAGACTCACC CAGGGAGAAG ATTTACTATG TATTTGAAAG GCTTCCCCAA
    AATGGGCAAC TTCAGCTTAA GATAGGGAGG GACTGGGTTC CTCTCTCCCC TGGCATGAAA
    TGCACTCAGG AGGAAGTGGA TCTGAACTTG CTGAGATACA CACACACCGG GGCAATGGAT
    TCCCAGAATC AAGATAGCTT CACCTTCTAC CTTTGGGATG GCAACAACAG GTCCCCTGCT
    CTTGACTGTC AAATCACCAT CAAGGACATG GAAAAAGGTG ATATTGTCAT CCTTACAAAA
    CCACTCGTGG TGTCTAAAGG TGATAGAGGT TTCTTAACAA CCACCACTCT CCTGGCTGTG
    GACGGAACAG ACAAGCCTGA GGAACTGCTC TATGTCATCA CCTCCCCTCC ACGATATGGC
    CAGATCGAAT ATGTTCACTA TCCTGGAGTT CCCATTACAA ACTTCAGCCA AATGGATATA
    GCGGGGCAGA CAGTTTGCTA TGTACACAAG AGCAAGGTGA CTGTCTCCAG TGACAGATTC
    AGATTCATCA TCAGCAATGG ACTGCGGACC GAGCACGGGG TGTTTGAGAT CACACTGGAG
    ACTGTGGACA GAGCCCTGCC TGTGGTAACC AGGAACAAGG GGTTGAGACT GGCCCAAGGG
    GCCGTGGGCC TGCTTTCCCC TGACCTCCTT CAGCTGACCG ACCCTGATAC ACCTGTGGAG
    AACCTCACCT TCCTCTTGGT TCAGCTTCCC CAGCATGGCC AGCTCTACCT GTGGGGGACA
    GGGCTACTTC AACACAATTT CACCCAGCAG GATGTGGACA GCAGGAATGT GGCCTATCGG
    CACTCAGGAG GGGACTCCCA GACTGACTGC TTTACTTTCA TGGCCACAGA CGGGACAAAC
    CAAGGCTTTA TTGTGAATGG GAGAGTATGG GAAGAACCTG TTTTATTCAC CATTCAGGTA
    GACCAATTGG ACAAAACAGC TCCTCGTATC ACACTCTTGC ATTCCCCTTC TCAAGTGGGG
    CTCCTGAAAA ATGGCTGCTA CGGGATTTAC ATCACTTCCC GCGTGTTGAA GGCATCAGAC
    CCTGACACTG AGGACGATCA GATCATCTTT AAAATTCTAC AAGGCCCAAA ACATGGACAT
    CTGGAGAACA CAACAACAGG TGAATTTATC CATGAGAAAT TTAGCCAAAA GGACTTAAAC
    AGTAAGACTA TTCTTTACAT CATAAACCCA TCTTTGGAAG TAAATTCAGA TACCGTGGAA
    TTTCAAATCA TGGACCCCAC AGGGAACTCG GCCACTCCTC AAATTTTGGA ACTGAAGTGG
    TCTCATATTG AATGGTCACA GACCGAATAT GAAGTCTGTG AGAATGTGGG TGTGTTGCCC
    TTGGAAATTA TCAGAAGGGG ATATTCCATG GACTCGGCCT TTGTGGGTAT AAAGGTCAAC
    CAAGTGTCAG CTGCAGATGG AAAAGATTTC ACCGTGATTC CATCTAAACT GATTCAGTTT
    GACCCAGGAA TGTCAACTAA GATGTGGAAT ATAGCAATTA CCTATGACGG ATTAGAGGAA
    GATGATGAGG TCTTTGAAGT AATTCTGAAC TCCCCTGTGA ATGCAGTTCT TGGCACAAAG
    ACAAAAGCTG CAGTGAAAAT TTTGGACTCA AAAGGAGGAC AATGCCATCC TTCATATTCC
    TCCAACCAAA GCAAGCACAG CACATGGGAG AAGGGCATTT GGCATCTGCT GCCCCCAGGG
    TCTTCCTCAT CCACCACTTC TGGTTCCTTT CATCTGGAAA GAAGACCTCT TCCATCTTCC
    ATGCAGCTAG CAGTCACCAG GGGAGACACC CTGCGGGGCT TTGATTCTAC AGATCTTTCT
    CAAAGGAAGC TTAGGACCCG TGGGAATGGC AAAACAGTTC GTCCATCCTC TGTTTATAGA
    AATGGAACAG ACATCATCTA TAATTATCAT GGGATAGTTT CCTTGAAACT AGAGGATGAC
    AGCTTCCCAA CTCACAAAAG GAAGGCCAAA GTATCCATCA TTAGTCAGCC ACAAAAGACA
    ATCAAAGTGG CAGAACTGCC TCAAGCAGAT AAGGTGGAAT CCACAACTGA CTCACACTTC
    CCCAGACAGG ACCAGTTGCC CTCATTTCCA AAGAACTGCA CTCTGGAATT AAAGGGACTC
    TTCCATTTTG AAGAAGGCAT CCAGAAGCTG TATCAGTGCA ATGGGATTGC CTGGAAAGCC
    TGGAGTCCCC AAACCAAGGA TGTGGAAGAA AAATCCTGTC CAGCCGGGTG GCACCAGCAC
    TCAGGCTACT GTCACATCTT GATCACAGAG CAGAAAGGCA CCTGGAATGC GGCTGCCCAA
    GCTTGCAGGG AACAATACCT GGGCAACCTT GTAACTGTAT TCTCCAGGCA GCACATGCGG
    TGGCTCTGGG ACATTGGTGA GAGAAAGTCC TTTTGGATAG GTTTGAACGA CCAAGTGCAT
    GCTGGCCACT GGGAGTGGAT CGGTGGTGAA CCTGTTGCCT TCACCAATGG GAGAAGAGGG
    CCCTCTCAAC GCTCCAAGCT TGGAAAGAGC TGTGTTTTGG TTCAAAGACA AGGGAAATGG
    CAAACAAAAG ATTGTAGGAG AGCCAAACCT CATAATTATG TGTGTTCCAG AAAACTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OPc88757
    Clone ID Related Accession (Same CDS sequence) XM_016960628.1
    Accession Version XM_016960628.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6516bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-01
    Organism Pan troglodytes(chimpanzee)
    Product FRAS1-related extracellular matrix protein 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006476.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGAACTCTC TGAGTTGGGG GGCTGCGAAT GCCGTGCTGC TGCTGCTCCT CCTGGCCTGG 
    GCCAGCCCCA CCTTCATCAG CATCAACCGT GGGGTGAGGG TGATGAAGGG CCACTCTGCC
    TTCCTGTCAG GAGATGACCT GAAGTTTGCC ATCCCTAAAG AGAAAGATGC CTGCAAAGTG
    GAAGTTGTGA TGAATGAGCC AATAACCCAG AGGGTTGGGA AACTCACTCC ACAGGTCTTT
    GACTGCCATT TCCTTCCCAA TGAAGTCAAG TATGTTCACA ATGGTTGTCC AATTCTTGAT
    GAAGACACAG TGAAGCTTAG ACTTTACAGA TTTACTGAAA GAGATACCTT CATAGAAACC
    TTTATCCTGT GGGTCTATCT CCTGGAACCA GACTGTAACA TCATCCATAT GAGTAACAAT
    GTGCTGGAGG TGCCTGAATT CAATGGCCTG TCCCAAGCGA TTGATAAAAA TCTGCTCAGA
    TTCGATTATG ATAGGATGGC TAGCCTGGAA TGTACGGTCA GCCTGGACAC TGCGAGAACT
    CGGCTGCCAG CCCATGGCCA GATGGTTCTC GGGGAGCCTC GACCAGAAGA ACCTCGTGGA
    GATCAGCCAC ACAGTTTTTT CCCAGAGTCC CAACTGAGAG CAAAACTGAA GTGTCCAGGT
    GGAAGCTGTA CCCCAGGATT AAAGAAAATA GGAAGCCTCA AAGTGAGCTG TGAGGAGTTC
    CTGCTGATGG GCCTTCGTTA TCAGCATCTG GATCCCCCTT CACCCAACAT TGATTATATC
    TCCATCCAAC TGGACCTCAC AGATACCAGG AGTAAAATTG TGTACAAGTC AGAGAGTGCG
    TGGCTGCCTG TCTATATCAG AGCTGGAATT CCGAATCAGA TTCCAAAGGC TGCATTCATG
    GCCATGTTTA TTCTGGAAGT GGATCAGTTC ATCCTGACCT CCTTGACTAC ATCAGTTCTG
    GACTGTGAAG AAGATGAGAC CCCTAAACCC TTGCTGGTGT TCAACATTAC TAAAGCCCCG
    CTCCAGGGCT ATGTGACTCA CCTGTTGGAT CACACCAGAC CAATCTCCTC ATTCACCTGG
    AAAGATCTCA GTGACATGCA GATCGCCTAT CAGCCACCAA ACAGCAGCCA TTCTGAGAGG
    AGACATGATG AGGTAGAATT GGAGGTATAC GACTTCTTCT TTGAAAGGAG TGCACCTATG
    ACAGTCCACA TCTCCATCAG AACAGCAGAT ACAAATGCCC CCCGTGTATC CTGGAATACA
    GGTCTGAATC TCCTTGAGGG GCAGTCTCGA GCCATCACTT GGGAACAGTT TCAGGTTGTC
    GACAATGACG ACATCGGTGC TGTCCGGCTA GTCACCGTTG GTGGCCTGCA GCATGGATGG
    CTGACTTTAA GAGGGGGGAA AGGATTTCTC TTCACCGTGG CTGACCTCCA GGCTGGAGTT
    GTTCGCTATC ATCATGATGA CAGCGACTCC ACCAAAGACT TCGTGGTCTT CCGGATATTT
    GATGGCCATC ACAGCATCCG TCACAAATTC CCCATCAACG TCTTGCCCAA AGATGATAGT
    CCCCCGTTCC TCATAACCAA TGTTGTCATT GAACTGGAGG AGGGGCAGAC CATCCTGATC
    CAGGGATCCA TGCTGCGAGC TTCAGATGTG GATGCCAGTG ATGACTACAT CTTCTTCAAT
    ATCACAAAGC CTCCACAGGC TGGGGAGATC ATGAAGAAGC CAGGGCCAGG ACTGATAGGC
    TATCCTGTCC CTGGCTTCCT TCAGAGGGAT TTGTTTAATG GAATCATTTA TTATCGTCAT
    TTTGGTGGAG AAATCTTTGA AGATTCTTTT CAATTTGTCC TGTGGGACAG CCATGAACCT
    CCAAATCTTT CAGTGCCACA GGTGGCAACA ATCCATGTAA CTCCAGTGGA TGACCAGCTT
    CCAAAAGAGG CTCCTGGAGT TTCTCGGCAT TTGGTTGTCA AGGAAACTGA GGTGGCCTAT
    ATAACTAAGA AACAGCTACA TTTTATAGAT TCAGAATCAT ATGACAGGGA GCTGGTCTAC
    ACGATAACTA CTCCTCCATT TTTCTCCTTC AGCCACAGAC ACTTGGATGC TGGGAAATTA
    TTTATGGTGG ACAGCATACC AAAAGTAGTT AAGAATCCTA CGGCCCTGGA GCTGAGGTCA
    TTCACTCAGC ATGCTGTGAA CTATATGAAA GTGGCCTACA TGCCCCCCAT GCAAGACATT
    GGTCCCCATT GCAGAGATGT CCAGTTCACA TTTTCTGTCA GTAACCAACA TGGCGGCACT
    TTGCATGGGA TCTGCTTTAA CATTACAATC CTCCCAGTGG ACAATCAAGT TCCTGAGGCG
    TTCACCAACC CTCTGAAAGT GACTGAGGGA GGTCAAAGCA TCATCAGCAC AGAGCACATT
    CTGATTTCTG ATGCAGATAC CAAGCTGGAC AATATTGACC TCTCCCTGCG GGAATTGCCT
    CTGCACGGAA GGGTGGAGCT GAATGGATTT CCTCTAAATT CAGGGGGCAC ATTTTCTTGG
    GGCGATCTCC ATACCTTAAA AGTTAGGTAT CAACATGATG GAACTGAAGT TCTTCAGGAT
    GACCTACTCT TGGAGGTCAC CGATGGCACA AATTCAGCAG AATTTGTTCT ACATGTTGAG
    GTATTCCCTG TCAACGATGA GCCACCAGTC TTAAAGGCTG ACCTCATGCC TGTCATGAAT
    TGCTCAGAGG GAGGAGAGGT GGTCATCACC TCTGAATACA TTTTTGCTAC TGATGTGGAC
    AGCGATAACT TGAAGTTGAT GTTTGTGATT GCTCGCGAAC CTCAGCATGG GGTGGTGAGG
    AGAGCTGGAG TCACAGTGGA TCAGTTCTCT CAGAGAGATG TTATCTCAGA GGCCGTGACA
    TACAAACACA CAGGTGGCGA GATTGGCATG ATGCCCTGTT TTGACACCAT TACATTGGTG
    GTTTCGGATG GAGAGGCTGG CCCTTTTGTG AATGGCTGTT GCTACAATGG ACCCAATCCA
    TCTGTTCCGC TTCATGCGTC CTTTCCAGTG TATGATCTCA ACATCACGGT ATACCCAGTA
    GACAACCAGC CACCTTCCGT TGCAATAGGT CCCATGTTTG TTGTAGATGA GGGCTGCTCA
    ACAGCCCTTA CTGTTAACCA TTTGTCCGCC ACTGACCCTG ACACTGCAGC AGATGACTTG
    GAATTTGTTT TGGTTTCTCC TCCTCAGTTT GGCTACCTCG AAAATATACT CCCTTCTGTG
    GGTTTTGAAA AAAGCAATAT TGGCATAAGT ATAGATTCAT TTCAGTGGAA AGACATGAAC
    GCTTTTCACA TTAACTATGT GCAGTCCAGG CATCTGAGGA TAGAACCAAC TGCCGACCAG
    TTCACGGTGT ACGTCACAGA TGGGAAGCAA CACTCCTTGG AGATACCATT TTCTATTATA
    ATCAACCCCA CAAATGATGA AGCTCCTGAC TTTGTAGTGC AGAATATTAC TGTGTGCGAG
    GGTCAGATGA AAGAGCTGGA CTCTTCCATC ATCAGCGCTG TGGACCTGGA CATTCCCCAG
    GATGCCCTGC TGTTCAGCAT CACTCAAAAG CCACGCCATG GCCTCCTCAT CGATAGGGGG
    TTTAGCAAAG ACTTCTCTGA GAATAAGCAG CCAGCCAACC CTCACCAGAA ACATGTACCT
    GTTCACAGTT TTTCCATGGA ACTCCTCAAG ACTGGAATGA GGTTGACGTA CATGCATGAT
    GACTCAGAGA GCCTTGCTGA TGATTTTACA ATCCGTTTGT CAGATGGGAA ACATAAGATA
    CTTAAAACCA TTTCAATAGA GGTCATCCCA GTTAATGATG AAAAACCAAT GCTGAGCAAG
    AAGGCTGAAA TTGCAATGAA TATGGGTGAA ACTCGTATTA TTTCCAGTGC TATTCTTTCA
    GCCATAGATG AAGACTCACC CAGGGAGAAG ATTTACTATG TATTTGAAAG GCTTCCCCAA
    AATGGGCAAC TTCAGCTTAA GATAGGGAGG GACTGGGTTC CTCTCTCCCC TGGCATGAAA
    TGCACTCAGG AGGAAGTGGA TCTGAACTTG CTGAGATACA CACACACCGG GGCAATGGAT
    TCCCAGAATC AAGATAGCTT CACCTTCTAC CTTTGGGATG GCAACAACAG GTCCCCTGCT
    CTTGACTGTC AAATCACCAT CAAGGACATG GAAAAAGGTG ATATTGTCAT CCTTACAAAA
    CCACTCGTGG TGTCTAAAGG TGATAGAGGT TTCTTAACAA CCACCACTCT CCTGGCTGTG
    GACGGAACAG ACAAGCCTGA GGAACTGCTC TATGTCATCA CCTCCCCTCC ACGATATGGC
    CAGATCGAAT ATGTTCACTA TCCTGGAGTT CCCATTACAA ACTTCAGCCA AATGGATATA
    GCGGGGCAGA CAGTTTGCTA TGTACACAAG AGCAAGGTGA CTGTCTCCAG TGACAGATTC
    AGATTCATCA TCAGCAATGG ACTGCGGACC GAGCACGGGG TGTTTGAGAT CACACTGGAG
    ACTGTGGACA GAGCCCTGCC TGTGGTAACC AGGAACAAGG GGTTGAGACT GGCCCAAGGG
    GCCGTGGGCC TGCTTTCCCC TGACCTCCTT CAGCTGACCG ACCCTGATAC ACCTGTGGAG
    AACCTCACCT TCCTCTTGGT TCAGCTTCCC CAGCATGGCC AGCTCTACCT GTGGGGGACA
    GGGCTACTTC AACACAATTT CACCCAGCAG GATGTGGACA GCAGGAATGT GGCCTATCGG
    CACTCAGGAG GGGACTCCCA GACTGACTGC TTTACTTTCA TGGCCACAGA CGGGACAAAC
    CAAGGCTTTA TTGTGAATGG GAGAGTATGG GAAGAACCTG TTTTATTCAC CATTCAGGTA
    GACCAATTGG ACAAAACAGC TCCTCGTATC ACACTCTTGC ATTCCCCTTC TCAAGTGGGG
    CTCCTGAAAA ATGGCTGCTA CGGGATTTAC ATCACTTCCC GCGTGTTGAA GGCATCAGAC
    CCTGACACTG AGGACGATCA GATCATCTTT AAAATTCTAC AAGGCCCAAA ACATGGACAT
    CTGGAGAACA CAACAACAGG TGAATTTATC CATGAGAAAT TTAGCCAAAA GGACTTAAAC
    AGTAAGACTA TTCTTTACAT CATAAACCCA TCTTTGGAAG TAAATTCAGA TACCGTGGAA
    TTTCAAATCA TGGACCCCAC AGGGAACTCG GCCACTCCTC AAATTTTGGA ACTGAAGTGG
    TCTCATATTG AATGGTCACA GACCGAATAT GAAGTCTGTG AGAATGTGGG TGTGTTGCCC
    TTGGAAATTA TCAGAAGGGG ATATTCCATG GACTCGGCCT TTGTGGGTAT AAAGGTCAAC
    CAAGTGTCAG CTGCAGATGG AAAAGATTTC ACCGTGATTC CATCTAAACT GATTCAGTTT
    GACCCAGGAA TGTCAACTAA GATGTGGAAT ATAGCAATTA CCTATGACGG ATTAGAGGAA
    GATGATGAGG TCTTTGAAGT AATTCTGAAC TCCCCTGTGA ATGCAGTTCT TGGCACAAAG
    ACAAAAGCTG CAGTGAAAAT TTTGGACTCA AAAGGAGGAC AATGCCATCC TTCATATTCC
    TCCAACCAAA GCAAGCACAG CACATGGGAG AAGGGCATTT GGCATCTGCT GCCCCCAGGG
    TCTTCCTCAT CCACCACTTC TGGTTCCTTT CATCTGGAAA GAAGACCTCT TCCATCTTCC
    ATGCAGCTAG CAGTCACCAG GGGAGACACC CTGCGGGGCT TTGATTCTAC AGATCTTTCT
    CAAAGGAAGC TTAGGACCCG TGGGAATGGC AAAACAGTTC GTCCATCCTC TGTTTATAGA
    AATGGAACAG ACATCATCTA TAATTATCAT GGGATAGTTT CCTTGAAACT AGAGGATGAC
    AGCTTCCCAA CTCACAAAAG GAAGGCCAAA GTATCCATCA TTAGTCAGCC ACAAAAGACA
    ATCAAAGTGG CAGAACTGCC TCAAGCAGAT AAGGTGGAAT CCACAACTGA CTCACACTTC
    CCCAGACAGG ACCAGTTGCC CTCATTTCCA AAGAACTGCA CTCTGGAATT AAAGGGACTC
    TTCCATTTTG AAGAAGGCAT CCAGAAGCTG TATCAGTGCA ATGGGATTGC CTGGAAAGCC
    TGGAGTCCCC AAACCAAGGA TGTGGAAGAA AAATCCTGTC CAGCCGGGTG GCACCAGCAC
    TCAGGCTACT GTCACATCTT GATCACAGAG CAGAAAGGCA CCTGGAATGC GGCTGCCCAA
    GCTTGCAGGG AACAATACCT GGGCAACCTT GTAACTGTAT TCTCCAGGCA GCACATGCGG
    TGGCTCTGGG ACATTGGTTT GAACGACCAA GTGCATGCTG GCCACTGGGA GTGGATCGGT
    GGTGAACCTG TTGCCTTCAC CAATGGGAGA AGAGGGCCCT CTCAACGCTC CAAGCTTGGA
    AAGAGCTGTG TTTTGGTTCA AAGACAAGGG AAATGGCAAA CAAAAGATTG TAGGAGAGCC
    AAACCTCATA ATTATGTGTG TTCCAGAAAA CTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016816117.1
    CDS2126..8641
    Translation

    Target ORF information:

    RefSeq Version XM_016960628.1
    Organism Pan troglodytes(chimpanzee)
    Definition Pan troglodytes FRAS1 related extracellular matrix 1 (FREM1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_016960628.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGAACTCTC TGAGTTGGGG GGCTGCGAAT GCCGTGCTGC TGCTGCTCCT CCTGGCCTGG 
    GCCAGCCCCA CCTTCATCAG CATCAACCGT GGGGTGAGGG TGATGAAGGG CCACTCTGCC
    TTCCTGTCAG GAGATGACCT GAAGTTTGCC ATCCCTAAAG AGAAAGATGC CTGCAAAGTG
    GAAGTTGTGA TGAATGAGCC AATAACCCAG AGGGTTGGGA AACTCACTCC ACAGGTCTTT
    GACTGCCATT TCCTTCCCAA TGAAGTCAAG TATGTTCACA ATGGTTGTCC AATTCTTGAT
    GAAGACACAG TGAAGCTTAG ACTTTACAGA TTTACTGAAA GAGATACCTT CATAGAAACC
    TTTATCCTGT GGGTCTATCT CCTGGAACCA GACTGTAACA TCATCCATAT GAGTAACAAT
    GTGCTGGAGG TGCCTGAATT CAATGGCCTG TCCCAAGCGA TTGATAAAAA TCTGCTCAGA
    TTCGATTATG ATAGGATGGC TAGCCTGGAA TGTACGGTCA GCCTGGACAC TGCGAGAACT
    CGGCTGCCAG CCCATGGCCA GATGGTTCTC GGGGAGCCTC GACCAGAAGA ACCTCGTGGA
    GATCAGCCAC ACAGTTTTTT CCCAGAGTCC CAACTGAGAG CAAAACTGAA GTGTCCAGGT
    GGAAGCTGTA CCCCAGGATT AAAGAAAATA GGAAGCCTCA AAGTGAGCTG TGAGGAGTTC
    CTGCTGATGG GCCTTCGTTA TCAGCATCTG GATCCCCCTT CACCCAACAT TGATTATATC
    TCCATCCAAC TGGACCTCAC AGATACCAGG AGTAAAATTG TGTACAAGTC AGAGAGTGCG
    TGGCTGCCTG TCTATATCAG AGCTGGAATT CCGAATCAGA TTCCAAAGGC TGCATTCATG
    GCCATGTTTA TTCTGGAAGT GGATCAGTTC ATCCTGACCT CCTTGACTAC ATCAGTTCTG
    GACTGTGAAG AAGATGAGAC CCCTAAACCC TTGCTGGTGT TCAACATTAC TAAAGCCCCG
    CTCCAGGGCT ATGTGACTCA CCTGTTGGAT CACACCAGAC CAATCTCCTC ATTCACCTGG
    AAAGATCTCA GTGACATGCA GATCGCCTAT CAGCCACCAA ACAGCAGCCA TTCTGAGAGG
    AGACATGATG AGGTAGAATT GGAGGTATAC GACTTCTTCT TTGAAAGGAG TGCACCTATG
    ACAGTCCACA TCTCCATCAG AACAGCAGAT ACAAATGCCC CCCGTGTATC CTGGAATACA
    GGTCTGAATC TCCTTGAGGG GCAGTCTCGA GCCATCACTT GGGAACAGTT TCAGGTTGTC
    GACAATGACG ACATCGGTGC TGTCCGGCTA GTCACCGTTG GTGGCCTGCA GCATGGATGG
    CTGACTTTAA GAGGGGGGAA AGGATTTCTC TTCACCGTGG CTGACCTCCA GGCTGGAGTT
    GTTCGCTATC ATCATGATGA CAGCGACTCC ACCAAAGACT TCGTGGTCTT CCGGATATTT
    GATGGCCATC ACAGCATCCG TCACAAATTC CCCATCAACG TCTTGCCCAA AGATGATAGT
    CCCCCGTTCC TCATAACCAA TGTTGTCATT GAACTGGAGG AGGGGCAGAC CATCCTGATC
    CAGGGATCCA TGCTGCGAGC TTCAGATGTG GATGCCAGTG ATGACTACAT CTTCTTCAAT
    ATCACAAAGC CTCCACAGGC TGGGGAGATC ATGAAGAAGC CAGGGCCAGG ACTGATAGGC
    TATCCTGTCC CTGGCTTCCT TCAGAGGGAT TTGTTTAATG GAATCATTTA TTATCGTCAT
    TTTGGTGGAG AAATCTTTGA AGATTCTTTT CAATTTGTCC TGTGGGACAG CCATGAACCT
    CCAAATCTTT CAGTGCCACA GGTGGCAACA ATCCATGTAA CTCCAGTGGA TGACCAGCTT
    CCAAAAGAGG CTCCTGGAGT TTCTCGGCAT TTGGTTGTCA AGGAAACTGA GGTGGCCTAT
    ATAACTAAGA AACAGCTACA TTTTATAGAT TCAGAATCAT ATGACAGGGA GCTGGTCTAC
    ACGATAACTA CTCCTCCATT TTTCTCCTTC AGCCACAGAC ACTTGGATGC TGGGAAATTA
    TTTATGGTGG ACAGCATACC AAAAGTAGTT AAGAATCCTA CGGCCCTGGA GCTGAGGTCA
    TTCACTCAGC ATGCTGTGAA CTATATGAAA GTGGCCTACA TGCCCCCCAT GCAAGACATT
    GGTCCCCATT GCAGAGATGT CCAGTTCACA TTTTCTGTCA GTAACCAACA TGGCGGCACT
    TTGCATGGGA TCTGCTTTAA CATTACAATC CTCCCAGTGG ACAATCAAGT TCCTGAGGCG
    TTCACCAACC CTCTGAAAGT GACTGAGGGA GGTCAAAGCA TCATCAGCAC AGAGCACATT
    CTGATTTCTG ATGCAGATAC CAAGCTGGAC AATATTGACC TCTCCCTGCG GGAATTGCCT
    CTGCACGGAA GGGTGGAGCT GAATGGATTT CCTCTAAATT CAGGGGGCAC ATTTTCTTGG
    GGCGATCTCC ATACCTTAAA AGTTAGGTAT CAACATGATG GAACTGAAGT TCTTCAGGAT
    GACCTACTCT TGGAGGTCAC CGATGGCACA AATTCAGCAG AATTTGTTCT ACATGTTGAG
    GTATTCCCTG TCAACGATGA GCCACCAGTC TTAAAGGCTG ACCTCATGCC TGTCATGAAT
    TGCTCAGAGG GAGGAGAGGT GGTCATCACC TCTGAATACA TTTTTGCTAC TGATGTGGAC
    AGCGATAACT TGAAGTTGAT GTTTGTGATT GCTCGCGAAC CTCAGCATGG GGTGGTGAGG
    AGAGCTGGAG TCACAGTGGA TCAGTTCTCT CAGAGAGATG TTATCTCAGA GGCCGTGACA
    TACAAACACA CAGGTGGCGA GATTGGCATG ATGCCCTGTT TTGACACCAT TACATTGGTG
    GTTTCGGATG GAGAGGCTGG CCCTTTTGTG AATGGCTGTT GCTACAATGG ACCCAATCCA
    TCTGTTCCGC TTCATGCGTC CTTTCCAGTG TATGATCTCA ACATCACGGT ATACCCAGTA
    GACAACCAGC CACCTTCCGT TGCAATAGGT CCCATGTTTG TTGTAGATGA GGGCTGCTCA
    ACAGCCCTTA CTGTTAACCA TTTGTCCGCC ACTGACCCTG ACACTGCAGC AGATGACTTG
    GAATTTGTTT TGGTTTCTCC TCCTCAGTTT GGCTACCTCG AAAATATACT CCCTTCTGTG
    GGTTTTGAAA AAAGCAATAT TGGCATAAGT ATAGATTCAT TTCAGTGGAA AGACATGAAC
    GCTTTTCACA TTAACTATGT GCAGTCCAGG CATCTGAGGA TAGAACCAAC TGCCGACCAG
    TTCACGGTGT ACGTCACAGA TGGGAAGCAA CACTCCTTGG AGATACCATT TTCTATTATA
    ATCAACCCCA CAAATGATGA AGCTCCTGAC TTTGTAGTGC AGAATATTAC TGTGTGCGAG
    GGTCAGATGA AAGAGCTGGA CTCTTCCATC ATCAGCGCTG TGGACCTGGA CATTCCCCAG
    GATGCCCTGC TGTTCAGCAT CACTCAAAAG CCACGCCATG GCCTCCTCAT CGATAGGGGG
    TTTAGCAAAG ACTTCTCTGA GAATAAGCAG CCAGCCAACC CTCACCAGAA ACATGTACCT
    GTTCACAGTT TTTCCATGGA ACTCCTCAAG ACTGGAATGA GGTTGACGTA CATGCATGAT
    GACTCAGAGA GCCTTGCTGA TGATTTTACA ATCCGTTTGT CAGATGGGAA ACATAAGATA
    CTTAAAACCA TTTCAATAGA GGTCATCCCA GTTAATGATG AAAAACCAAT GCTGAGCAAG
    AAGGCTGAAA TTGCAATGAA TATGGGTGAA ACTCGTATTA TTTCCAGTGC TATTCTTTCA
    GCCATAGATG AAGACTCACC CAGGGAGAAG ATTTACTATG TATTTGAAAG GCTTCCCCAA
    AATGGGCAAC TTCAGCTTAA GATAGGGAGG GACTGGGTTC CTCTCTCCCC TGGCATGAAA
    TGCACTCAGG AGGAAGTGGA TCTGAACTTG CTGAGATACA CACACACCGG GGCAATGGAT
    TCCCAGAATC AAGATAGCTT CACCTTCTAC CTTTGGGATG GCAACAACAG GTCCCCTGCT
    CTTGACTGTC AAATCACCAT CAAGGACATG GAAAAAGGTG ATATTGTCAT CCTTACAAAA
    CCACTCGTGG TGTCTAAAGG TGATAGAGGT TTCTTAACAA CCACCACTCT CCTGGCTGTG
    GACGGAACAG ACAAGCCTGA GGAACTGCTC TATGTCATCA CCTCCCCTCC ACGATATGGC
    CAGATCGAAT ATGTTCACTA TCCTGGAGTT CCCATTACAA ACTTCAGCCA AATGGATATA
    GCGGGGCAGA CAGTTTGCTA TGTACACAAG AGCAAGGTGA CTGTCTCCAG TGACAGATTC
    AGATTCATCA TCAGCAATGG ACTGCGGACC GAGCACGGGG TGTTTGAGAT CACACTGGAG
    ACTGTGGACA GAGCCCTGCC TGTGGTAACC AGGAACAAGG GGTTGAGACT GGCCCAAGGG
    GCCGTGGGCC TGCTTTCCCC TGACCTCCTT CAGCTGACCG ACCCTGATAC ACCTGTGGAG
    AACCTCACCT TCCTCTTGGT TCAGCTTCCC CAGCATGGCC AGCTCTACCT GTGGGGGACA
    GGGCTACTTC AACACAATTT CACCCAGCAG GATGTGGACA GCAGGAATGT GGCCTATCGG
    CACTCAGGAG GGGACTCCCA GACTGACTGC TTTACTTTCA TGGCCACAGA CGGGACAAAC
    CAAGGCTTTA TTGTGAATGG GAGAGTATGG GAAGAACCTG TTTTATTCAC CATTCAGGTA
    GACCAATTGG ACAAAACAGC TCCTCGTATC ACACTCTTGC ATTCCCCTTC TCAAGTGGGG
    CTCCTGAAAA ATGGCTGCTA CGGGATTTAC ATCACTTCCC GCGTGTTGAA GGCATCAGAC
    CCTGACACTG AGGACGATCA GATCATCTTT AAAATTCTAC AAGGCCCAAA ACATGGACAT
    CTGGAGAACA CAACAACAGG TGAATTTATC CATGAGAAAT TTAGCCAAAA GGACTTAAAC
    AGTAAGACTA TTCTTTACAT CATAAACCCA TCTTTGGAAG TAAATTCAGA TACCGTGGAA
    TTTCAAATCA TGGACCCCAC AGGGAACTCG GCCACTCCTC AAATTTTGGA ACTGAAGTGG
    TCTCATATTG AATGGTCACA GACCGAATAT GAAGTCTGTG AGAATGTGGG TGTGTTGCCC
    TTGGAAATTA TCAGAAGGGG ATATTCCATG GACTCGGCCT TTGTGGGTAT AAAGGTCAAC
    CAAGTGTCAG CTGCAGATGG AAAAGATTTC ACCGTGATTC CATCTAAACT GATTCAGTTT
    GACCCAGGAA TGTCAACTAA GATGTGGAAT ATAGCAATTA CCTATGACGG ATTAGAGGAA
    GATGATGAGG TCTTTGAAGT AATTCTGAAC TCCCCTGTGA ATGCAGTTCT TGGCACAAAG
    ACAAAAGCTG CAGTGAAAAT TTTGGACTCA AAAGGAGGAC AATGCCATCC TTCATATTCC
    TCCAACCAAA GCAAGCACAG CACATGGGAG AAGGGCATTT GGCATCTGCT GCCCCCAGGG
    TCTTCCTCAT CCACCACTTC TGGTTCCTTT CATCTGGAAA GAAGACCTCT TCCATCTTCC
    ATGCAGCTAG CAGTCACCAG GGGAGACACC CTGCGGGGCT TTGATTCTAC AGATCTTTCT
    CAAAGGAAGC TTAGGACCCG TGGGAATGGC AAAACAGTTC GTCCATCCTC TGTTTATAGA
    AATGGAACAG ACATCATCTA TAATTATCAT GGGATAGTTT CCTTGAAACT AGAGGATGAC
    AGCTTCCCAA CTCACAAAAG GAAGGCCAAA GTATCCATCA TTAGTCAGCC ACAAAAGACA
    ATCAAAGTGG CAGAACTGCC TCAAGCAGAT AAGGTGGAAT CCACAACTGA CTCACACTTC
    CCCAGACAGG ACCAGTTGCC CTCATTTCCA AAGAACTGCA CTCTGGAATT AAAGGGACTC
    TTCCATTTTG AAGAAGGCAT CCAGAAGCTG TATCAGTGCA ATGGGATTGC CTGGAAAGCC
    TGGAGTCCCC AAACCAAGGA TGTGGAAGAA AAATCCTGTC CAGCCGGGTG GCACCAGCAC
    TCAGGCTACT GTCACATCTT GATCACAGAG CAGAAAGGCA CCTGGAATGC GGCTGCCCAA
    GCTTGCAGGG AACAATACCT GGGCAACCTT GTAACTGTAT TCTCCAGGCA GCACATGCGG
    TGGCTCTGGG ACATTGGTTT GAACGACCAA GTGCATGCTG GCCACTGGGA GTGGATCGGT
    GGTGAACCTG TTGCCTTCAC CAATGGGAGA AGAGGGCCCT CTCAACGCTC CAAGCTTGGA
    AAGAGCTGTG TTTTGGTTCA AAGACAAGGG AAATGGCAAA CAAAAGATTG TAGGAGAGCC
    AAACCTCATA ATTATGTGTG TTCCAGAAAA CTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OPc88758
    Clone ID Related Accession (Same CDS sequence) XM_016960629.1
    Accession Version XM_016960629.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6390bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-01
    Organism Pan troglodytes(chimpanzee)
    Product FRAS1-related extracellular matrix protein 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006476.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    ATGAACTCTC TGAGTTGGGG GGCTGCGAAT GCCGTGCTGC TGCTGCTCCT CCTGGCCTGG 
    GCCAGCCCCA CCTTCATCAG CATCAACCGT GGGGTGAGGG TGATGAAGGG CCACTCTGCC
    TTCCTGTCAG GAGATGACCT GAAGTTTGCC ATCCCTAAAG AGAAAGATGC CTGCAAAGTG
    GAAGTTGTGA TGAATGAGCC AATAACCCAG AGGGTTGGGA AACTCACTCC ACAGGTCTTT
    GACTGCCATT TCCTTCCCAA TGAAGTCAAG TATGTTCACA ATGGTTGTCC AATTCTTGAT
    GAAGACACAG TGAAGCTTAG ACTTTACAGA TTTACTGAAA GAGATACCTT CATAGAAACC
    TTTATCCTGT GGGTCTATCT CCTGGAACCA GACTGTAACA TCATCCATAT GAGTAACAAT
    GTGCTGGAGG TGCCTGAATT CAATGGCCTG TCCCAAGCGA TTGATAAAAA TCTGCTCAGA
    TTCGATTATG ATAGGATGGC TAGCCTGGAA TGTACGGTCA GCCTGGACAC TGCGAGAACT
    CGGCTGCCAG CCCATGGCCA GATGGTTCTC GGGGAGCCTC GACCAGAAGA ACCTCGTGGA
    GATCAGCCAC ACAGTTTTTT CCCAGAGTCC CAACTGAGAG CAAAACTGAA GTGTCCAGGT
    GGAAGCTGTA CCCCAGGATT AAAGAAAATA GGAAGCCTCA AAGTGAGCTG TGAGGAGTTC
    CTGCTGATGG GCCTTCGTTA TCAGCATCTG GATCCCCCTT CACCCAACAT TGATTATATC
    TCCATCCAAC TGGACCTCAC AGATACCAGG AGTAAAATTG TGTACAAGTC AGAGAGTGCG
    TGGCTGCCTG TCTATATCAG AGCTGGAATT CCGAATCAGA TTCCAAAGGC TGCATTCATG
    GCCATGTTTA TTCTGGAAGT GGATCAGTTC ATCCTGACCT CCTTGACTAC ATCAGTTCTG
    GACTGTGAAG AAGATGAGAC CCCTAAACCC TTGCTGGTGT TCAACATTAC TAAAGCCCCG
    CTCCAGGGCT ATGTGACTCA CCTGTTGGAT CACACCAGAC CAATCTCCTC ATTCACCTGG
    AAAGATCTCA GTGACATGCA GATCGCCTAT CAGCCACCAA ACAGCAGCCA TTCTGAGAGG
    AGACATGATG AGGTAGAATT GGAGGTATAC GACTTCTTCT TTGAAAGGAG TGCACCTATG
    ACAGTCCACA TCTCCATCAG AACAGCAGAT ACAAATGCCC CCCGTGTATC CTGGAATACA
    GGTCTGAATC TCCTTGAGGG GCAGTCTCGA GCCATCACTT GGGAACAGTT TCAGGTTGTC
    GACAATGACG ACATCGGTGC TGTCCGGCTA GTCACCGTTG GTGGCCTGCA GCATGGATGG
    CTGACTTTAA GAGGGGGGAA AGGATTTCTC TTCACCGTGG CTGACCTCCA GGCTGGAGTT
    GTTCGCTATC ATCATGATGA CAGCGACTCC ACCAAAGACT TCGTGGTCTT CCGGATATTT
    GATGGCCATC ACAGCATCCG TCACAAATTC CCCATCAACG TCTTGCCCAA AGATGATAGT
    CCCCCGTTCC TCATAACCAA TGTTGTCATT GAACTGGAGG AGGGGCAGAC CATCCTGATC
    CAGGGATCCA TGCTGCGAGC TTCAGATGTG GATGCCAGTG ATGACTACAT CTTCTTCAAT
    ATCACAAAGC CTCCACAGGC TGGGGAGATC ATGAAGAAGC CAGGGCCAGG ACTGATAGGC
    TATCCTGTCC CTGGCTTCCT TCAGAGGGAT TTGTTTAATG GAATCATTTA TTATCGTCAT
    TTTGGTGGAG AAATCTTTGA AGATTCTTTT CAATTTGTCC TGTGGGACAG CCATGAACCT
    CCAAATCTTT CAGTGCCACA GGTGGCAACA ATCCATGTAA CTCCAGTGGA TGACCAGCTT
    CCAAAAGAGG CTCCTGGAGT TTCTCGGCAT TTGGTTGTCA AGGAAACTGA GGTGGCCTAT
    ATAACTAAGA AACAGCTACA TTTTATAGAT TCAGAATCAT ATGACAGGGA GCTGGTCTAC
    ACGATAACTA CTCCTCCATT TTTCTCCTTC AGCCACAGAC ACTTGGATGC TGGGAAATTA
    TTTATGGTGG ACAGCATACC AAAAGTAGTT AAGAATCCTA CGGCCCTGGA GCTGAGGTCA
    TTCACTCAGC ATGCTGTGAA CTATATGAAA GTGGCCTACA TGCCCCCCAT GCAAGACATT
    GGTCCCCATT GCAGAGATGT CCAGTTCACA TTTTCTGTCA GTAACCAACA TGGCGGCACT
    TTGCATGGGA TCTGCTTTAA CATTACAATC CTCCCAGTGG ACAATCAAGT TCCTGAGGCG
    TTCACCAACC CTCTGAAAGT GACTGAGGGA GGTCAAAGCA TCATCAGCAC AGAGCACATT
    CTGATTTCTG ATGCAGATAC CAAGCTGGAC AATATTGACC TCTCCCTGCG GGAATTGCCT
    CTGCACGGAA GGGTGGAGCT GAATGGATTT CCTCTAAATT CAGGGGGCAC ATTTTCTTGG
    GGCGATCTCC ATACCTTAAA AGTTAGGTAT CAACATGATG GAACTGAAGT TCTTCAGGAT
    GACCTACTCT TGGAGGTCAC CGATGGCACA AATTCAGCAG AATTTGTTCT ACATGTTGAG
    GTATTCCCTG TCAACGATGA GCCACCAGTC TTAAAGGCTG ACCTCATGCC TGTCATGAAT
    TGCTCAGAGG GAGGAGAGGT GGTCATCACC TCTGAATACA TTTTTGCTAC TGATGTGGAC
    AGCGATAACT TGAAGTTGAT GTTTGTGATT GCTCGCGAAC CTCAGCATGG GGTGGTGAGG
    AGAGCTGGAG TCACAGTGGA TCAGTTCTCT CAGAGAGATG TTATCTCAGA GGCCGTGACA
    TACAAACACA CAGGTGGCGA GATTGGCATG ATGCCCTGTT TTGACACCAT TACATTGGTG
    GTTTCGGATG GAGAGGCTGG CCCTTTTGTG AATGGCTGTT GCTACAATGG ACCCAATCCA
    TCTGTTCCGC TTCATGCGTC CTTTCCAGTG TATGATCTCA ACATCACGGT ATACCCAGTA
    GACAACCAGC CACCTTCCGT TGCAATAGGT CCCATGTTTG TTGTAGATGA GGGCTGCTCA
    ACAGCCCTTA CTGTTAACCA TTTGTCCGCC ACTGACCCTG ACACTGCAGC AGATGACTTG
    GAATTTGTTT TGGTTTCTCC TCCTCAGTTT GGCTACCTCG AAAATATACT CCCTTCTGTG
    GGTTTTGAAA AAAGCAATAT TGGCATAAGT ATAGATTCAT TTCAGTGGAA AGACATGAAC
    GCTTTTCACA TTAACTATGT GCAGTCCAGG CATCTGAGGA TAGAACCAAC TGCCGACCAG
    TTCACGGTGT ACGTCACAGA TGGGAAGCAA CACTCCTTGG AGATACCATT TTCTATTATA
    ATCAACCCCA CAAATGATGA AGCTCCTGAC TTTGTAGTGC AGAATATTAC TGTGTGCGAG
    GGTCAGATGA AAGAGCTGGA CTCTTCCATC ATCAGCGCTG TGGACCTGGA CATTCCCCAG
    GATGCCCTGC TGTTCAGCAT CACTCAAAAG CCACGCCATG GCCTCCTCAT CGATAGGGGG
    TTTAGCAAAG ACTTCTCTGA GAATAAGCAG CCAGCCAACC CTCACCAGAA ACATGTACCT
    GTTCACAGTT TTTCCATGGA ACTCCTCAAG ACTGGAATGA GGTTGACGTA CATGCATGAT
    GACTCAGAGA GCCTTGCTGA TGATTTTACA ATCCGTTTGT CAGATGGGAA ACATAAGATA
    CTTAAAACCA TTTCAATAGA GGTCATCCCA GTTAATGATG AAAAACCAAT GCTGAGCAAG
    AAGGCTGAAA TTGCAATGAA TATGGGTGAA ACTCGTATTA TTTCCAGTGC TATTCTTTCA
    GCCATAGATG AAGACTCACC CAGGGAGAAG ATTTACTATG TATTTGAAAG GCTTCCCCAA
    AATGGGCAAC TTCAGCTTAA GATAGGGAGG GACTGGGTTC CTCTCTCCCC TGGCATGAAA
    TGCACTCAGG AGGAAGTGGA TCTGAACTTG CTGAGATACA CACACACCGG GGCAATGGAT
    TCCCAGAATC AAGATAGCTT CACCTTCTAC CTTTGGGATG GCAACAACAG GTCCCCTGCT
    CTTGACTGTC AAATCACCAT CAAGGACATG GAAAAAGGTG ATATTGTCAT CCTTACAAAA
    CCACTCGTGG TGTCTAAAGG TGATAGAGGT TTCTTAACAA CCACCACTCT CCTGGCTGTG
    GACGGAACAG ACAAGCCTGA GGAACTGCTC TATGTCATCA CCTCCCCTCC ACGATATGGC
    CAGATCGAAT ATGTTCACTA TCCTGGAGTT CCCATTACAA ACTTCAGCCA AATGGATATA
    GCGGGGCAGA CAGTTTGCTA TGTACACAAG AGCAAGGTGA CTGTCTCCAG TGACAGATTC
    AGATTCATCA TCAGCAATGG ACTGCGGACC GAGCACGGGG TGTTTGAGAT CACACTGGAG
    ACTGTGGACA GAGCCCTGCC TGTGGTAACC AGGAACAAGG GGTTGAGACT GGCCCAAGGG
    GCCGTGGGCC TGCTTTCCCC TGACCTCCTT CAGCTGACCG ACCCTGATAC ACCTGTGGAG
    AACCTCACCT TCCTCTTGGT TCAGCTTCCC CAGCATGGCC AGCTCTACCT GTGGGGGACA
    GGGCTACTTC AACACAATTT CACCCAGCAG GATGTGGACA GCAGGAATGT GGCCTATCGG
    CACTCAGGAG GGGACTCCCA GACTGACTGC TTTACTTTCA TGGCCACAGA CGGGACAAAC
    CAAGGCTTTA TTGTGAATGG GAGAGTATGG GAAGAACCTG TTTTATTCAC CATTCAGGTA
    GACCAATTGG ACAAAACAGC TCCTCGTATC ACACTCTTGC ATTCCCCTTC TCAAGTGGGG
    CTCCTGAAAA ATGGCTGCTA CGGGATTTAC ATCACTTCCC GCGTGTTGAA GGCATCAGAC
    CCTGACACTG AGGACGATCA GATCATCTTT AAAATTCTAC AAGGCCCAAA ACATGGACAT
    CTGGAGAACA CAACAACAGG TGAATTTATC CATGAGAAAT TTAGCCAAAA GGACTTAAAC
    AGTAAGACTA TTCTTTACAT CATAAACCCA TCTTTGGAAG TAAATTCAGA TACCGTGGAA
    TTTCAAATCA TGGACCCCAC AGGGAACTCG GCCACTCCTC AAATTTTGGA ACTGAAGTGG
    TCTCATATTG AATGGTCACA GACCGAATAT GAAGTCTGTG AGAATGTGGG TGTGTTGCCC
    TTGGAAATTA TCAGAAGGGG ATATTCCATG GACTCGGCCT TTGTGGGTAT AAAGGTCAAC
    CAAGTGTCAG CTGCAGATGG AAAAGATTTC ACCGTGATTC CATCTAAACT GATTCAGTTT
    GACCCAGGAC AATGCCATCC TTCATATTCC TCCAACCAAA GCAAGCACAG CACATGGGAG
    AAGGGCATTT GGCATCTGCT GCCCCCAGGG TCTTCCTCAT CCACCACTTC TGGTTCCTTT
    CATCTGGAAA GAAGACCTCT TCCATCTTCC ATGCAGCTAG CAGTCACCAG GGGAGACACC
    CTGCGGGGCT TTGATTCTAC AGATCTTTCT CAAAGGAAGC TTAGGACCCG TGGGAATGGC
    AAAACAGTTC GTCCATCCTC TGTTTATAGA AATGGAACAG ACATCATCTA TAATTATCAT
    GGGATAGTTT CCTTGAAACT AGAGGATGAC AGCTTCCCAA CTCACAAAAG GAAGGCCAAA
    GTATCCATCA TTAGTCAGCC ACAAAAGACA ATCAAAGTGG CAGAACTGCC TCAAGCAGAT
    AAGGTGGAAT CCACAACTGA CTCACACTTC CCCAGACAGG ACCAGTTGCC CTCATTTCCA
    AAGAACTGCA CTCTGGAATT AAAGGGACTC TTCCATTTTG AAGAAGGCAT CCAGAAGCTG
    TATCAGTGCA ATGGGATTGC CTGGAAAGCC TGGAGTCCCC AAACCAAGGA TGTGGAAGAA
    AAATCCTGTC CAGCCGGGTG GCACCAGCAC TCAGGCTACT GTCACATCTT GATCACAGAG
    CAGAAAGGCA CCTGGAATGC GGCTGCCCAA GCTTGCAGGG AACAATACCT GGGCAACCTT
    GTAACTGTAT TCTCCAGGCA GCACATGCGG TGGCTCTGGG ACATTGGTGA GAGAAAGTCC
    TTTTGGATAG GTTTGAACGA CCAAGTGCAT GCTGGCCACT GGGAGTGGAT CGGTGGTGAA
    CCTGTTGCCT TCACCAATGG GAGAAGAGGG CCCTCTCAAC GCTCCAAGCT TGGAAAGAGC
    TGTGTTTTGG TTCAAAGACA AGGGAAATGG CAAACAAAAG ATTGTAGGAG AGCCAAACCT
    CATAATTATG TGTGTTCCAG AAAACTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016816118.1
    CDS2126..8515
    Translation

    Target ORF information:

    RefSeq Version XM_016960629.1
    Organism Pan troglodytes(chimpanzee)
    Definition Pan troglodytes FRAS1 related extracellular matrix 1 (FREM1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_016960629.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    ATGAACTCTC TGAGTTGGGG GGCTGCGAAT GCCGTGCTGC TGCTGCTCCT CCTGGCCTGG 
    GCCAGCCCCA CCTTCATCAG CATCAACCGT GGGGTGAGGG TGATGAAGGG CCACTCTGCC
    TTCCTGTCAG GAGATGACCT GAAGTTTGCC ATCCCTAAAG AGAAAGATGC CTGCAAAGTG
    GAAGTTGTGA TGAATGAGCC AATAACCCAG AGGGTTGGGA AACTCACTCC ACAGGTCTTT
    GACTGCCATT TCCTTCCCAA TGAAGTCAAG TATGTTCACA ATGGTTGTCC AATTCTTGAT
    GAAGACACAG TGAAGCTTAG ACTTTACAGA TTTACTGAAA GAGATACCTT CATAGAAACC
    TTTATCCTGT GGGTCTATCT CCTGGAACCA GACTGTAACA TCATCCATAT GAGTAACAAT
    GTGCTGGAGG TGCCTGAATT CAATGGCCTG TCCCAAGCGA TTGATAAAAA TCTGCTCAGA
    TTCGATTATG ATAGGATGGC TAGCCTGGAA TGTACGGTCA GCCTGGACAC TGCGAGAACT
    CGGCTGCCAG CCCATGGCCA GATGGTTCTC GGGGAGCCTC GACCAGAAGA ACCTCGTGGA
    GATCAGCCAC ACAGTTTTTT CCCAGAGTCC CAACTGAGAG CAAAACTGAA GTGTCCAGGT
    GGAAGCTGTA CCCCAGGATT AAAGAAAATA GGAAGCCTCA AAGTGAGCTG TGAGGAGTTC
    CTGCTGATGG GCCTTCGTTA TCAGCATCTG GATCCCCCTT CACCCAACAT TGATTATATC
    TCCATCCAAC TGGACCTCAC AGATACCAGG AGTAAAATTG TGTACAAGTC AGAGAGTGCG
    TGGCTGCCTG TCTATATCAG AGCTGGAATT CCGAATCAGA TTCCAAAGGC TGCATTCATG
    GCCATGTTTA TTCTGGAAGT GGATCAGTTC ATCCTGACCT CCTTGACTAC ATCAGTTCTG
    GACTGTGAAG AAGATGAGAC CCCTAAACCC TTGCTGGTGT TCAACATTAC TAAAGCCCCG
    CTCCAGGGCT ATGTGACTCA CCTGTTGGAT CACACCAGAC CAATCTCCTC ATTCACCTGG
    AAAGATCTCA GTGACATGCA GATCGCCTAT CAGCCACCAA ACAGCAGCCA TTCTGAGAGG
    AGACATGATG AGGTAGAATT GGAGGTATAC GACTTCTTCT TTGAAAGGAG TGCACCTATG
    ACAGTCCACA TCTCCATCAG AACAGCAGAT ACAAATGCCC CCCGTGTATC CTGGAATACA
    GGTCTGAATC TCCTTGAGGG GCAGTCTCGA GCCATCACTT GGGAACAGTT TCAGGTTGTC
    GACAATGACG ACATCGGTGC TGTCCGGCTA GTCACCGTTG GTGGCCTGCA GCATGGATGG
    CTGACTTTAA GAGGGGGGAA AGGATTTCTC TTCACCGTGG CTGACCTCCA GGCTGGAGTT
    GTTCGCTATC ATCATGATGA CAGCGACTCC ACCAAAGACT TCGTGGTCTT CCGGATATTT
    GATGGCCATC ACAGCATCCG TCACAAATTC CCCATCAACG TCTTGCCCAA AGATGATAGT
    CCCCCGTTCC TCATAACCAA TGTTGTCATT GAACTGGAGG AGGGGCAGAC CATCCTGATC
    CAGGGATCCA TGCTGCGAGC TTCAGATGTG GATGCCAGTG ATGACTACAT CTTCTTCAAT
    ATCACAAAGC CTCCACAGGC TGGGGAGATC ATGAAGAAGC CAGGGCCAGG ACTGATAGGC
    TATCCTGTCC CTGGCTTCCT TCAGAGGGAT TTGTTTAATG GAATCATTTA TTATCGTCAT
    TTTGGTGGAG AAATCTTTGA AGATTCTTTT CAATTTGTCC TGTGGGACAG CCATGAACCT
    CCAAATCTTT CAGTGCCACA GGTGGCAACA ATCCATGTAA CTCCAGTGGA TGACCAGCTT
    CCAAAAGAGG CTCCTGGAGT TTCTCGGCAT TTGGTTGTCA AGGAAACTGA GGTGGCCTAT
    ATAACTAAGA AACAGCTACA TTTTATAGAT TCAGAATCAT ATGACAGGGA GCTGGTCTAC
    ACGATAACTA CTCCTCCATT TTTCTCCTTC AGCCACAGAC ACTTGGATGC TGGGAAATTA
    TTTATGGTGG ACAGCATACC AAAAGTAGTT AAGAATCCTA CGGCCCTGGA GCTGAGGTCA
    TTCACTCAGC ATGCTGTGAA CTATATGAAA GTGGCCTACA TGCCCCCCAT GCAAGACATT
    GGTCCCCATT GCAGAGATGT CCAGTTCACA TTTTCTGTCA GTAACCAACA TGGCGGCACT
    TTGCATGGGA TCTGCTTTAA CATTACAATC CTCCCAGTGG ACAATCAAGT TCCTGAGGCG
    TTCACCAACC CTCTGAAAGT GACTGAGGGA GGTCAAAGCA TCATCAGCAC AGAGCACATT
    CTGATTTCTG ATGCAGATAC CAAGCTGGAC AATATTGACC TCTCCCTGCG GGAATTGCCT
    CTGCACGGAA GGGTGGAGCT GAATGGATTT CCTCTAAATT CAGGGGGCAC ATTTTCTTGG
    GGCGATCTCC ATACCTTAAA AGTTAGGTAT CAACATGATG GAACTGAAGT TCTTCAGGAT
    GACCTACTCT TGGAGGTCAC CGATGGCACA AATTCAGCAG AATTTGTTCT ACATGTTGAG
    GTATTCCCTG TCAACGATGA GCCACCAGTC TTAAAGGCTG ACCTCATGCC TGTCATGAAT
    TGCTCAGAGG GAGGAGAGGT GGTCATCACC TCTGAATACA TTTTTGCTAC TGATGTGGAC
    AGCGATAACT TGAAGTTGAT GTTTGTGATT GCTCGCGAAC CTCAGCATGG GGTGGTGAGG
    AGAGCTGGAG TCACAGTGGA TCAGTTCTCT CAGAGAGATG TTATCTCAGA GGCCGTGACA
    TACAAACACA CAGGTGGCGA GATTGGCATG ATGCCCTGTT TTGACACCAT TACATTGGTG
    GTTTCGGATG GAGAGGCTGG CCCTTTTGTG AATGGCTGTT GCTACAATGG ACCCAATCCA
    TCTGTTCCGC TTCATGCGTC CTTTCCAGTG TATGATCTCA ACATCACGGT ATACCCAGTA
    GACAACCAGC CACCTTCCGT TGCAATAGGT CCCATGTTTG TTGTAGATGA GGGCTGCTCA
    ACAGCCCTTA CTGTTAACCA TTTGTCCGCC ACTGACCCTG ACACTGCAGC AGATGACTTG
    GAATTTGTTT TGGTTTCTCC TCCTCAGTTT GGCTACCTCG AAAATATACT CCCTTCTGTG
    GGTTTTGAAA AAAGCAATAT TGGCATAAGT ATAGATTCAT TTCAGTGGAA AGACATGAAC
    GCTTTTCACA TTAACTATGT GCAGTCCAGG CATCTGAGGA TAGAACCAAC TGCCGACCAG
    TTCACGGTGT ACGTCACAGA TGGGAAGCAA CACTCCTTGG AGATACCATT TTCTATTATA
    ATCAACCCCA CAAATGATGA AGCTCCTGAC TTTGTAGTGC AGAATATTAC TGTGTGCGAG
    GGTCAGATGA AAGAGCTGGA CTCTTCCATC ATCAGCGCTG TGGACCTGGA CATTCCCCAG
    GATGCCCTGC TGTTCAGCAT CACTCAAAAG CCACGCCATG GCCTCCTCAT CGATAGGGGG
    TTTAGCAAAG ACTTCTCTGA GAATAAGCAG CCAGCCAACC CTCACCAGAA ACATGTACCT
    GTTCACAGTT TTTCCATGGA ACTCCTCAAG ACTGGAATGA GGTTGACGTA CATGCATGAT
    GACTCAGAGA GCCTTGCTGA TGATTTTACA ATCCGTTTGT CAGATGGGAA ACATAAGATA
    CTTAAAACCA TTTCAATAGA GGTCATCCCA GTTAATGATG AAAAACCAAT GCTGAGCAAG
    AAGGCTGAAA TTGCAATGAA TATGGGTGAA ACTCGTATTA TTTCCAGTGC TATTCTTTCA
    GCCATAGATG AAGACTCACC CAGGGAGAAG ATTTACTATG TATTTGAAAG GCTTCCCCAA
    AATGGGCAAC TTCAGCTTAA GATAGGGAGG GACTGGGTTC CTCTCTCCCC TGGCATGAAA
    TGCACTCAGG AGGAAGTGGA TCTGAACTTG CTGAGATACA CACACACCGG GGCAATGGAT
    TCCCAGAATC AAGATAGCTT CACCTTCTAC CTTTGGGATG GCAACAACAG GTCCCCTGCT
    CTTGACTGTC AAATCACCAT CAAGGACATG GAAAAAGGTG ATATTGTCAT CCTTACAAAA
    CCACTCGTGG TGTCTAAAGG TGATAGAGGT TTCTTAACAA CCACCACTCT CCTGGCTGTG
    GACGGAACAG ACAAGCCTGA GGAACTGCTC TATGTCATCA CCTCCCCTCC ACGATATGGC
    CAGATCGAAT ATGTTCACTA TCCTGGAGTT CCCATTACAA ACTTCAGCCA AATGGATATA
    GCGGGGCAGA CAGTTTGCTA TGTACACAAG AGCAAGGTGA CTGTCTCCAG TGACAGATTC
    AGATTCATCA TCAGCAATGG ACTGCGGACC GAGCACGGGG TGTTTGAGAT CACACTGGAG
    ACTGTGGACA GAGCCCTGCC TGTGGTAACC AGGAACAAGG GGTTGAGACT GGCCCAAGGG
    GCCGTGGGCC TGCTTTCCCC TGACCTCCTT CAGCTGACCG ACCCTGATAC ACCTGTGGAG
    AACCTCACCT TCCTCTTGGT TCAGCTTCCC CAGCATGGCC AGCTCTACCT GTGGGGGACA
    GGGCTACTTC AACACAATTT CACCCAGCAG GATGTGGACA GCAGGAATGT GGCCTATCGG
    CACTCAGGAG GGGACTCCCA GACTGACTGC TTTACTTTCA TGGCCACAGA CGGGACAAAC
    CAAGGCTTTA TTGTGAATGG GAGAGTATGG GAAGAACCTG TTTTATTCAC CATTCAGGTA
    GACCAATTGG ACAAAACAGC TCCTCGTATC ACACTCTTGC ATTCCCCTTC TCAAGTGGGG
    CTCCTGAAAA ATGGCTGCTA CGGGATTTAC ATCACTTCCC GCGTGTTGAA GGCATCAGAC
    CCTGACACTG AGGACGATCA GATCATCTTT AAAATTCTAC AAGGCCCAAA ACATGGACAT
    CTGGAGAACA CAACAACAGG TGAATTTATC CATGAGAAAT TTAGCCAAAA GGACTTAAAC
    AGTAAGACTA TTCTTTACAT CATAAACCCA TCTTTGGAAG TAAATTCAGA TACCGTGGAA
    TTTCAAATCA TGGACCCCAC AGGGAACTCG GCCACTCCTC AAATTTTGGA ACTGAAGTGG
    TCTCATATTG AATGGTCACA GACCGAATAT GAAGTCTGTG AGAATGTGGG TGTGTTGCCC
    TTGGAAATTA TCAGAAGGGG ATATTCCATG GACTCGGCCT TTGTGGGTAT AAAGGTCAAC
    CAAGTGTCAG CTGCAGATGG AAAAGATTTC ACCGTGATTC CATCTAAACT GATTCAGTTT
    GACCCAGGAC AATGCCATCC TTCATATTCC TCCAACCAAA GCAAGCACAG CACATGGGAG
    AAGGGCATTT GGCATCTGCT GCCCCCAGGG TCTTCCTCAT CCACCACTTC TGGTTCCTTT
    CATCTGGAAA GAAGACCTCT TCCATCTTCC ATGCAGCTAG CAGTCACCAG GGGAGACACC
    CTGCGGGGCT TTGATTCTAC AGATCTTTCT CAAAGGAAGC TTAGGACCCG TGGGAATGGC
    AAAACAGTTC GTCCATCCTC TGTTTATAGA AATGGAACAG ACATCATCTA TAATTATCAT
    GGGATAGTTT CCTTGAAACT AGAGGATGAC AGCTTCCCAA CTCACAAAAG GAAGGCCAAA
    GTATCCATCA TTAGTCAGCC ACAAAAGACA ATCAAAGTGG CAGAACTGCC TCAAGCAGAT
    AAGGTGGAAT CCACAACTGA CTCACACTTC CCCAGACAGG ACCAGTTGCC CTCATTTCCA
    AAGAACTGCA CTCTGGAATT AAAGGGACTC TTCCATTTTG AAGAAGGCAT CCAGAAGCTG
    TATCAGTGCA ATGGGATTGC CTGGAAAGCC TGGAGTCCCC AAACCAAGGA TGTGGAAGAA
    AAATCCTGTC CAGCCGGGTG GCACCAGCAC TCAGGCTACT GTCACATCTT GATCACAGAG
    CAGAAAGGCA CCTGGAATGC GGCTGCCCAA GCTTGCAGGG AACAATACCT GGGCAACCTT
    GTAACTGTAT TCTCCAGGCA GCACATGCGG TGGCTCTGGG ACATTGGTGA GAGAAAGTCC
    TTTTGGATAG GTTTGAACGA CCAAGTGCAT GCTGGCCACT GGGAGTGGAT CGGTGGTGAA
    CCTGTTGCCT TCACCAATGG GAGAAGAGGG CCCTCTCAAC GCTCCAAGCT TGGAAAGAGC
    TGTGTTTTGG TTCAAAGACA AGGGAAATGG CAAACAAAAG ATTGTAGGAG AGCCAAACCT
    CATAATTATG TGTGTTCCAG AAAACTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.