COL4A4 gene cDNA ORF clone, Pan troglodytes(chimpanzee)

mRNA	Protein	Name
XM_009444465.2	XP_009442740.2	collagen alpha-4(IV) chain isoform X1
XM_009444467.2	XP_009442742.2	collagen alpha-4(IV) chain isoform X3
XM_009444468.2	XP_009442743.2	collagen alpha-4(IV) chain isoform X5
XM_009444465.3	XP_009442740.3	collagen alpha-4(IV) chain isoform X1
XM_009444466.3	XP_009442741.3	collagen alpha-4(IV) chain isoform X2
XM_009444469.3	XP_009442744.3	collagen alpha-4(IV) chain isoform X6
XM_009444466.2	XP_009442741.2	collagen alpha-4(IV) chain isoform X2
XM_016950595.1	XP_016806084.1	collagen alpha-4(IV) chain isoform X8
XM_009444467.3	XP_009442742.3	collagen alpha-4(IV) chain isoform X3
XM_516128.4	XP_516128.3	collagen alpha-4(IV) chain isoform X1
XM_016950591.1	XP_016806080.1	collagen alpha-4(IV) chain isoform X1
XM_016950593.1	XP_016806082.1	collagen alpha-4(IV) chain isoform X6
XM_009444472.2	XP_009442747.2	collagen alpha-4(IV) chain isoform X8
XM_016950594.1	XP_016806083.1	collagen alpha-4(IV) chain isoform X8
XM_016950594.2	XP_016806083.2	collagen alpha-4(IV) chain isoform X8
XM_024355097.1	XP_024210865.1	collagen alpha-4(IV) chain isoform X9
XM_016950592.1	XP_016806081.1	collagen alpha-4(IV) chain isoform X4
XM_009444471.2	XP_009442746.2	collagen alpha-4(IV) chain isoform X7
XM_016950596.1	XP_016806085.1	collagen alpha-4(IV) chain isoform X8
XM_516128.5	XP_516128.4	collagen alpha-4(IV) chain isoform X1
XM_009444464.3	XP_009442739.3	collagen alpha-4(IV) chain isoform X1
XM_009444468.3	XP_009442743.3	collagen alpha-4(IV) chain isoform X5
XM_009444470.3	XP_009442745.3	collagen alpha-4(IV) chain isoform X6
XM_009444471.3	XP_009442746.3	collagen alpha-4(IV) chain isoform X7
XM_016950595.2	XP_016806084.2	collagen alpha-4(IV) chain isoform X8
XM_016950596.2	XP_016806085.2	collagen alpha-4(IV) chain isoform X8
XM_009444464.2	XP_009442739.2	collagen alpha-4(IV) chain isoform X1
XM_009444469.2	XP_009442744.2	collagen alpha-4(IV) chain isoform X6
XM_009444470.2	XP_009442745.2	collagen alpha-4(IV) chain isoform X6
XM_016950591.2	XP_016806080.2	collagen alpha-4(IV) chain isoform X1
XM_016950592.2	XP_016806081.2	collagen alpha-4(IV) chain isoform X4
XM_016950593.2	XP_016806082.2	collagen alpha-4(IV) chain isoform X6
XM_009444472.3	XP_009442747.3	collagen alpha-4(IV) chain isoform X8

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OPc104276	XM_009444472.3 Latest version!	Pan troglodytes collagen type IV alpha 4 chain (COL4A4), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OPc104276	XM_016950594.2 Latest version!	Pan troglodytes collagen type IV alpha 4 chain (COL4A4), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OPc104276	XM_016950595.2 Latest version!	Pan troglodytes collagen type IV alpha 4 chain (COL4A4), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OPc104276	XM_016950596.2 Latest version!	Pan troglodytes collagen type IV alpha 4 chain (COL4A4), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OPc104277	XM_024355097.1 Latest version!	Pan troglodytes collagen type IV alpha 4 chain (COL4A4), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OPc104269	XM_516128.5 Latest version!	Pan troglodytes collagen type IV alpha 4 chain (COL4A4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OPc104269	XM_009444465.3 Latest version!	Pan troglodytes collagen type IV alpha 4 chain (COL4A4), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OPc104269	XM_016950591.2 Latest version!	Pan troglodytes collagen type IV alpha 4 chain (COL4A4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OPc104269	XM_009444464.3 Latest version!	Pan troglodytes collagen type IV alpha 4 chain (COL4A4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OPc104270	XM_009444466.3 Latest version!	Pan troglodytes collagen type IV alpha 4 chain (COL4A4), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OPc104271	XM_009444467.3 Latest version!	Pan troglodytes collagen type IV alpha 4 chain (COL4A4), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OPc104272	XM_016950592.2 Latest version!	Pan troglodytes collagen type IV alpha 4 chain (COL4A4), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OPc104273	XM_009444468.3 Latest version!	Pan troglodytes collagen type IV alpha 4 chain (COL4A4), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OPc104274	XM_009444470.3 Latest version!	Pan troglodytes collagen type IV alpha 4 chain (COL4A4), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OPc104274	XM_009444469.3 Latest version!	Pan troglodytes collagen type IV alpha 4 chain (COL4A4), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OPc104274	XM_016950593.2 Latest version!	Pan troglodytes collagen type IV alpha 4 chain (COL4A4), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OPc104275	XM_009444471.3 Latest version!	Pan troglodytes collagen type IV alpha 4 chain (COL4A4), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OPc80763	XM_516128.4 Latest version!	Pan troglodytes collagen type IV alpha 4 (COL4A4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OPc80763	XM_016950591.1 Latest version!	Pan troglodytes collagen type IV alpha 4 (COL4A4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OPc80763	XM_009444465.2 Latest version!	Pan troglodytes collagen type IV alpha 4 (COL4A4), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OPc80763	XM_009444464.2 Latest version!	Pan troglodytes collagen type IV alpha 4 (COL4A4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OPc80764	XM_009444466.2 Latest version!	Pan troglodytes collagen type IV alpha 4 (COL4A4), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OPc80765	XM_009444467.2 Latest version!	Pan troglodytes collagen type IV alpha 4 (COL4A4), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OPc80766	XM_016950592.1 Latest version!	Pan troglodytes collagen type IV alpha 4 (COL4A4), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OPc80767	XM_009444468.2 Latest version!	Pan troglodytes collagen type IV alpha 4 (COL4A4), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OPc80768	XM_009444470.2 Latest version!	Pan troglodytes collagen type IV alpha 4 (COL4A4), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OPc80768	XM_009444469.2 Latest version!	Pan troglodytes collagen type IV alpha 4 (COL4A4), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OPc80768	XM_016950593.1 Latest version!	Pan troglodytes collagen type IV alpha 4 (COL4A4), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OPc80769	XM_009444471.2 Latest version!	Pan troglodytes collagen type IV alpha 4 (COL4A4), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OPc80770	XM_016950596.1 Latest version!	Pan troglodytes collagen type IV alpha 4 (COL4A4), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OPc80770	XM_016950595.1 Latest version!	Pan troglodytes collagen type IV alpha 4 (COL4A4), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OPc80770	XM_009444472.2 Latest version!	Pan troglodytes collagen type IV alpha 4 (COL4A4), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OPc80770	XM_016950594.1 Latest version!	Pan troglodytes collagen type IV alpha 4 (COL4A4), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00

COL4A4 ( XM_016950594.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016806083.2 Pan troglodytes collagen type IV alpha 4 chain (COL4A4), transcript variant X14, mRNA.

Price & Availability↑

CloneID	OPc104276
Clone ID Related Accession (Same CDS sequence)	XM_016950594.2 , XM_016950595.2 , XM_016950596.2 , XM_009444472.3
Accession Version	XM_016950594.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4245bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	collagen alpha-4(IV) chain isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036881.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_016950594.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGTCTC TGCACATAGT ACTAATGAGG TGCTCCTTCA GATTGACCAA GTCCTTGGCC 
ACAGATCCCT GGTCACTTAC ACTCATTCTC TTTTCTGTAC AATATGTATA TGGGAGTGGA 
AAGAAATACG TTGGTCCTTG TGGAGGAAGA GATTGCTCTG TTTGCCACTG TGTTCCTGAA 
AAGGGGTCTC GGGGTCCACC AGGACCACCA GGGCCACAGG GTCCAATTGG ACCCCTGGGA 
GCCCCAGGAC CCATTGGGCT TTCAGGAGAG AAAGGAATGA GAGGGGACCG CGGCCCTCCT 
GGAGCAGCAG GGGACAAAGG AGATAAGGGT TCAACTGGTG TTCCTGGATT TCCAGGTTTA 
GATGGCATAC CTGGGCACCC AGGGCCTCCT GGACCCAGAG GCAAACCTGG TATGAGTGGC 
CACAATGGCT CAAGAGGTGA CCCAGGGTTT CCAGGAGGAA GAGGAGCTCT TGGCCCAGGA 
GGCCCCCTAG GCCATCCTGG GGAAAAGGGA GAAAAAGGAA ATTCAGTGTT CATTTTAGGT 
GCCGTTAAAG GTATTCAGGG AGACAGAGGG GACCCAGGAC TGCCTGGCTT ACCAGGATCT 
TGGGGGGCAG GAGGACCGGC GGGTCCCACA GGATATCCTG GAGAGCCAGG GTTAGTGGGA 
CCTCCGGGCC AACCAGGGCG TCCAGGTTTG AAGGGAAATC CCGGTGTGGG AGTAAAGGGG 
CAAATGGGAG ACCCGGGTGA GGTTGGTCAG CAAGGTTCTC CTGGACCCAC CCTGTTGGTG 
GAGCCACCTG ACTTTCGTCT CTATAAAGGA GAAAAGGGTA TAAAAGGAAT TCCTGGAATG 
GTTGGACTGC CAGGACCACC AGGACGCAAG GGAGAATCTG ATATTGGGGC AAAAGGAGAA 
AAAGGTATTC CTGGATTTCC AGGGCCTCGG GGGGATCCTG GTTCCTATGG ATCTCCAGGT 
TTTCCAGGAT TAAAGGGAGA ACTAGGACTG GTTGGAGATC CTGGGCTATT TGGATTAATT 
GGCCCAAAGG GGGATCCTGG AAATCGAGGG CACCCAGGAC CACCAGGTGT TTTGGTGACT 
CCACCTCTTC CACTCAAAGG CCCACCAGGG GACCCAGGGT TCCCTGGCCG CTATGGAGAA 
ACAGGGGATG TTGGACCACC TGGTCCCCCA GGTCTCTTGG GCAGACCAGG GGAAGCCTGT 
GCAGGCATGA TAGGACCCCC TGGGCCACAA GGATTTCCTG GTCTTCCTGG GCTTCCAGGA 
GAAGCTGGTA TTCCTGGGAG ACCTGATTCT GCTCCAGGAA AACCAGGGAA GCCAGGATCA 
CCTGGCTTGC CTGGAGCACC AGGCCTGCAG GGCCTCCCAG GATCAAGTGT GATATACTGT 
AGTGTTGGGA ATCCTGGACC ACAAGGAATA AAAGGCAAAG TGGGTCCCCC AGGAGGAAGA 
GGCTCAAAAG GAGAAAAAGG AAATGAAGGA CTCTGTGCCT GTGAGCCTGG ACCCATGGGC 
CCCCCTGGCC CTCCAGGACT TCCTGGGAGG CAGGGGAGTA AGGGAGACTT GGGGCTCCCT 
GGCTGGCTTG GAACAAAAGG TGACCCAGGA CCTCCTGGTG CTGAAGGACC TCCAGGGCTA 
CCAGGAAAGC ATGGTGCCTC TGGACCACCT GGCAACAAAG GGGCGAAGGG TGACATGGTT 
GTATCAAGAG TTAAAGGGCA CAAAGGAGAA AGAGGTCCTG ATGGGCCCCC AGGATTTCCA 
GGGCAGCCAG GATCACATGG TCGGGATGGA CATGCTGGAG AAAAAGGGGA TCCAGGACCC 
CCAGGGGATC ATGAAGATGC GACCCCAGGT GGTAAAGGAT TTCCTGGACC TCTGGGCCCC 
CCGGGCAAAG CAGGACCTTT GGGGCCCCCA GGACTGGGAT TTCCTGGTCC ACCAGGACAG 
CGAGGCCACC CAGGAGTTCC AGGCCACCCA GGTGTGAGGG GCCCTGATGG CTTGAAGGGT 
CAGAAAGGTG ACACAATTTC TTGCAACGTA ACCTACCCTG GGAGGCACGG CCCTCCAGGT 
TTTGATGGAC CTCCAGGTCC GAAGGGATTT CCAGGTCCCC AAGGTGCCCC TGGGCTGAGT 
GGTTCAGATG GGCATAAAGG CAGACCTGGC ACACCAGGAA CAGCGGAAAT ACCAGGTCCA 
CCTGGTTTTC GTGGTGACAT GGGAGATCCG GGTTTTGGAG GTGAAAAGGG GTCCTCCCCT 
GCTGGGCCCC CAGGCCCTCC CGGCTCACCA GGAGTGAATG GTCAGAAAGG AATCCCGGGA 
GACCCTGCAT TTGGTCACCT GGGACCCCCG GGAAAGAGGG GTCTTTCAGG AGTGCCAGGG 
ATAAAAGGAC CCAGAGGTGA TCCGGGATGT CCAGGGGCTG AAGGGCCAGC TGGCATTCCT 
GGATTCCCAG GTCTCAAAGG TCCCAAAGGC AGAGAGGGAC ATGCTGGGTT TCCAGGTGTC 
CCAGGTCCAC CTGGCCATTC CTGTGAAAGA GGTGCTCCAG GGATACCAGG GCAACCGGGA 
CTCCCTGGGT ATCCAGGTAG CCCAGGTGCT CCAGGTGGGA AAGGACAGCT GGGAGATGTG 
GGGCCTCCCG GGCCAGCTGG AATGAAAGGC CTCCCTGGAC TCCCAGGATG GCCTGGGGCA 
CATGGTCCCC CAGGCCTCCC AGGAATCCCA GGTCCCTTTG GAGATGATGG GCTACCTGGT 
CCTCCAGGTC CAAAGGGACC CCAGGGGCTG CCTGGTTTCC CAGGTTTTCC CGGAGAAAGA 
GGAAAGCCTG GTGCAGAGGG ATGTCCTGGC ACAAAGGGAG AACCTGGAGA GAAGGGCATG 
TCTGGCCTTC CCGGAGACCG GGGACTGAGA GGGGCCAAAG GAGCCATAGG ACCTCCCGGA 
GATGAAGGAG AAATGGCTAT CATTTCCCAA AAGGGAACAC CTGGGGAACC TGGACCTCCT 
GGAGATGATG GATTCCCAGG AGAAAGAGGT GATAAAGGAA CTCCCGGGAT GCAAGGGAGA 
AGAGGAGAGC CGGGAAGATA CGGACCACCT GGATTTCAGA GAGGGGAACC TGGTGAGAAA 
GGTCAGCCAG GGCATCCTGG ACCCCCAGGC CCTCCAGGCT CAACTGGTCT AAGAGGGTTC 
ATTGGTTTTC CAGGACTTCC AGGTGACCAG GGTGAGCCAG GTTCTCCAGG TCCCCCTGGA 
TTTTCAGGAA TTGATGGAGC AAGAGGACCT AAAGGAAACA AAGGTGACCC TGCCAGTCAC 
TTTGGTCCAC CTGGTCCAAA GGGTGAGCCA GGTAGCCCTG GATGTCCAGG GCATTTTGGA 
GCATCTGGAG AGCAGGGCTT GCCTGGTATT CAAGGGCCCA GAGGATCACC TGGAAGGCCA 
GGGCCACCTG GCTCCTCTGG ACCACCAGGG TGCCCAGGTG ATCACGGGAT GCCTGGGCTG 
AGGGGACAGC CAGGAGAAAT GGGAGACCCT GGGCCAAGAG GCCTCCAGGG GGATCCAGGG 
ATACCAGGTC CTCCGGGAAT AAAAGGTCCC TCCGGATCAC CTGGCCTGAA CGGCTTGCAT 
GGATTGAAAG GTCAGAAAGG AACTAAAGGT GCTTCAGGTT TGCATGATGT GGGGCCACCT 
GGTCCAGTGG GAATACCTGG GCTAAAAGGG GAGAGAGGAG ACCCTGGGAG CCCAGGAATC 
TCTCCTCCAG GTCCTCGTGG AGAAAAAGGT CCCCCAGGAC CCCCAGGGAG TTCAGGACCA 
CCTGGTCCTG CAGGTGCCAC AGGAAGAGCT CCTAAGGACA TTCCTGACCC GGGTCCACCT 
GGAGATCAGG GACCTCCTGG TCCTGATGGC CCAAGAGGAG CACCTGGGCC TCCAGGCCTC 
CCTGGGAGTG TTGACCTTCT GAGAGGGGAG CCAGGTGACT GTGGTCTACC AGGGCCACCA 
GGTCCCCCTG GCCCACCAGG CCCTCCAGGA TACAAAGGCT TTCCAGGATG TGATGGAAAA 
GATGGCCAGA AAGGACCAAT GGGATTCCCG GGACCGCAGG GACCACATGG ATTTCCTGGG 
CCACCTGGAG AGAAGGGTTT ACCTGGACCT CCAGGGAGAA AAGGGCCCAC TGGTCTTCCA 
GGTCCCAGAG GTGAACCGGG GCCACCTGCA GATGTGGATG ACTGTCCCCG AATCCCAGGC 
CTTCCTGGGG CGCCAGGCAT GAGAGGACCA GAAGGAGCCA TGGGGCTCTC TGGAATGAGA 
GGCCCCCCAG GACCAGCTCA TGGGTGTGTT CTGAGGACCA AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016806083.2
CDS	422..4666
Translation	MWSLHIVLMRCSFRLTKSLATDPWSLTLILFSVQYVYGSGKKYVGPCGGRDCSVCHCVPEKGSRGPPGPPGPQGPIGPLGAPGPIGLSGEKGMRGDRGPPGAAGDKGDKGSTGVPGFPGLDGIPGHPGPPGPRGKPGMSGHNGSRGDPGFPGGRGALGPGGPLGHPGEKGEKGNSVFILGAVKGIQGDRGDPGLPGLPGSWGAGGPAGPTGYPGEPGLVGPPGQPGRPGLKGNPGVGVKGQMGDPGEVGQQGSPGPTLLVEPPDFRLYKGEKGIKGIPGMVGLPGPPGRKGESDIGAKGEKGIPGFPGPRGDPGSYGSPGFPGLKGELGLVGDPGLFGLIGPKGDPGNRGHPGPPGVLVTPPLPLKGPPGDPGFPGRYGETGDVGPPGPPGLLGRPGEACAGMIGPPGPQGFPGLPGLPGEAGIPGRPDSAPGKPGKPGSPGLPGAPGLQGLPGSSVIYCSVGNPGPQGIKGKVGPPGGRGSKGEKGNEGLCACEPGPMGPPGPPGLPGRQGSKGDLGLPGWLGTKGDPGPPGAEGPPGLPGKHGASGPPGNKGAKGDMVVSRVKGHKGERGPDGPPGFPGQPGSHGRDGHAGEKGDPGPPGDHEDATPGGKGFPGPLGPPGKAGPLGPPGLGFPGPPGQRGHPGVPGHPGVRGPDGLKGQKGDTISCNVTYPGRHGPPGFDGPPGPKGFPGPQGAPGLSGSDGHKGRPGTPGTAEIPGPPGFRGDMGDPGFGGEKGSSPAGPPGPPGSPGVNGQKGIPGDPAFGHLGPPGKRGLSGVPGIKGPRGDPGCPGAEGPAGIPGFPGLKGPKGREGHAGFPGVPGPPGHSCERGAPGIPGQPGLPGYPGSPGAPGGKGQLGDVGPPGPAGMKGLPGLPGWPGAHGPPGLPGIPGPFGDDGLPGPPGPKGPQGLPGFPGFPGERGKPGAEGCPGTKGEPGEKGMSGLPGDRGLRGAKGAIGPPGDEGEMAIISQKGTPGEPGPPGDDGFPGERGDKGTPGMQGRRGEPGRYGPPGFQRGEPGEKGQPGHPGPPGPPGSTGLRGFIGFPGLPGDQGEPGSPGPPGFSGIDGARGPKGNKGDPASHFGPPGPKGEPGSPGCPGHFGASGEQGLPGIQGPRGSPGRPGPPGSSGPPGCPGDHGMPGLRGQPGEMGDPGPRGLQGDPGIPGPPGIKGPSGSPGLNGLHGLKGQKGTKGASGLHDVGPPGPVGIPGLKGERGDPGSPGISPPGPRGEKGPPGPPGSSGPPGPAGATGRAPKDIPDPGPPGDQGPPGPDGPRGAPGPPGLPGSVDLLRGEPGDCGLPGPPGPPGPPGPPGYKGFPGCDGKDGQKGPMGFPGPQGPHGFPGPPGEKGLPGPPGRKGPTGLPGPRGEPGPPADVDDCPRIPGLPGAPGMRGPEGAMGLSGMRGPPGPAHGCVLRTK

Target ORF information:

RefSeq Version	XM_016950594.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes collagen type IV alpha 4 chain (COL4A4), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016950594.2

ORF Insert Sequence:

ATGTGGTCTC TGCACATAGT ACTAATGAGG TGCTCCTTCA GATTGACCAA GTCCTTGGCC 
ACAGATCCCT GGTCACTTAC ACTCATTCTC TTTTCTGTAC AATATGTATA TGGGAGTGGA 
AAGAAATACG TTGGTCCTTG TGGAGGAAGA GATTGCTCTG TTTGCCACTG TGTTCCTGAA 
AAGGGGTCTC GGGGTCCACC AGGACCACCA GGGCCACAGG GTCCAATTGG ACCCCTGGGA 
GCCCCAGGAC CCATTGGGCT TTCAGGAGAG AAAGGAATGA GAGGGGACCG CGGCCCTCCT 
GGAGCAGCAG GGGACAAAGG AGATAAGGGT TCAACTGGTG TTCCTGGATT TCCAGGTTTA 
GATGGCATAC CTGGGCACCC AGGGCCTCCT GGACCCAGAG GCAAACCTGG TATGAGTGGC 
CACAATGGCT CAAGAGGTGA CCCAGGGTTT CCAGGAGGAA GAGGAGCTCT TGGCCCAGGA 
GGCCCCCTAG GCCATCCTGG GGAAAAGGGA GAAAAAGGAA ATTCAGTGTT CATTTTAGGT 
GCCGTTAAAG GTATTCAGGG AGACAGAGGG GACCCAGGAC TGCCTGGCTT ACCAGGATCT 
TGGGGGGCAG GAGGACCGGC GGGTCCCACA GGATATCCTG GAGAGCCAGG GTTAGTGGGA 
CCTCCGGGCC AACCAGGGCG TCCAGGTTTG AAGGGAAATC CCGGTGTGGG AGTAAAGGGG 
CAAATGGGAG ACCCGGGTGA GGTTGGTCAG CAAGGTTCTC CTGGACCCAC CCTGTTGGTG 
GAGCCACCTG ACTTTCGTCT CTATAAAGGA GAAAAGGGTA TAAAAGGAAT TCCTGGAATG 
GTTGGACTGC CAGGACCACC AGGACGCAAG GGAGAATCTG ATATTGGGGC AAAAGGAGAA 
AAAGGTATTC CTGGATTTCC AGGGCCTCGG GGGGATCCTG GTTCCTATGG ATCTCCAGGT 
TTTCCAGGAT TAAAGGGAGA ACTAGGACTG GTTGGAGATC CTGGGCTATT TGGATTAATT 
GGCCCAAAGG GGGATCCTGG AAATCGAGGG CACCCAGGAC CACCAGGTGT TTTGGTGACT 
CCACCTCTTC CACTCAAAGG CCCACCAGGG GACCCAGGGT TCCCTGGCCG CTATGGAGAA 
ACAGGGGATG TTGGACCACC TGGTCCCCCA GGTCTCTTGG GCAGACCAGG GGAAGCCTGT 
GCAGGCATGA TAGGACCCCC TGGGCCACAA GGATTTCCTG GTCTTCCTGG GCTTCCAGGA 
GAAGCTGGTA TTCCTGGGAG ACCTGATTCT GCTCCAGGAA AACCAGGGAA GCCAGGATCA 
CCTGGCTTGC CTGGAGCACC AGGCCTGCAG GGCCTCCCAG GATCAAGTGT GATATACTGT 
AGTGTTGGGA ATCCTGGACC ACAAGGAATA AAAGGCAAAG TGGGTCCCCC AGGAGGAAGA 
GGCTCAAAAG GAGAAAAAGG AAATGAAGGA CTCTGTGCCT GTGAGCCTGG ACCCATGGGC 
CCCCCTGGCC CTCCAGGACT TCCTGGGAGG CAGGGGAGTA AGGGAGACTT GGGGCTCCCT 
GGCTGGCTTG GAACAAAAGG TGACCCAGGA CCTCCTGGTG CTGAAGGACC TCCAGGGCTA 
CCAGGAAAGC ATGGTGCCTC TGGACCACCT GGCAACAAAG GGGCGAAGGG TGACATGGTT 
GTATCAAGAG TTAAAGGGCA CAAAGGAGAA AGAGGTCCTG ATGGGCCCCC AGGATTTCCA 
GGGCAGCCAG GATCACATGG TCGGGATGGA CATGCTGGAG AAAAAGGGGA TCCAGGACCC 
CCAGGGGATC ATGAAGATGC GACCCCAGGT GGTAAAGGAT TTCCTGGACC TCTGGGCCCC 
CCGGGCAAAG CAGGACCTTT GGGGCCCCCA GGACTGGGAT TTCCTGGTCC ACCAGGACAG 
CGAGGCCACC CAGGAGTTCC AGGCCACCCA GGTGTGAGGG GCCCTGATGG CTTGAAGGGT 
CAGAAAGGTG ACACAATTTC TTGCAACGTA ACCTACCCTG GGAGGCACGG CCCTCCAGGT 
TTTGATGGAC CTCCAGGTCC GAAGGGATTT CCAGGTCCCC AAGGTGCCCC TGGGCTGAGT 
GGTTCAGATG GGCATAAAGG CAGACCTGGC ACACCAGGAA CAGCGGAAAT ACCAGGTCCA 
CCTGGTTTTC GTGGTGACAT GGGAGATCCG GGTTTTGGAG GTGAAAAGGG GTCCTCCCCT 
GCTGGGCCCC CAGGCCCTCC CGGCTCACCA GGAGTGAATG GTCAGAAAGG AATCCCGGGA 
GACCCTGCAT TTGGTCACCT GGGACCCCCG GGAAAGAGGG GTCTTTCAGG AGTGCCAGGG 
ATAAAAGGAC CCAGAGGTGA TCCGGGATGT CCAGGGGCTG AAGGGCCAGC TGGCATTCCT 
GGATTCCCAG GTCTCAAAGG TCCCAAAGGC AGAGAGGGAC ATGCTGGGTT TCCAGGTGTC 
CCAGGTCCAC CTGGCCATTC CTGTGAAAGA GGTGCTCCAG GGATACCAGG GCAACCGGGA 
CTCCCTGGGT ATCCAGGTAG CCCAGGTGCT CCAGGTGGGA AAGGACAGCT GGGAGATGTG 
GGGCCTCCCG GGCCAGCTGG AATGAAAGGC CTCCCTGGAC TCCCAGGATG GCCTGGGGCA 
CATGGTCCCC CAGGCCTCCC AGGAATCCCA GGTCCCTTTG GAGATGATGG GCTACCTGGT 
CCTCCAGGTC CAAAGGGACC CCAGGGGCTG CCTGGTTTCC CAGGTTTTCC CGGAGAAAGA 
GGAAAGCCTG GTGCAGAGGG ATGTCCTGGC ACAAAGGGAG AACCTGGAGA GAAGGGCATG 
TCTGGCCTTC CCGGAGACCG GGGACTGAGA GGGGCCAAAG GAGCCATAGG ACCTCCCGGA 
GATGAAGGAG AAATGGCTAT CATTTCCCAA AAGGGAACAC CTGGGGAACC TGGACCTCCT 
GGAGATGATG GATTCCCAGG AGAAAGAGGT GATAAAGGAA CTCCCGGGAT GCAAGGGAGA 
AGAGGAGAGC CGGGAAGATA CGGACCACCT GGATTTCAGA GAGGGGAACC TGGTGAGAAA 
GGTCAGCCAG GGCATCCTGG ACCCCCAGGC CCTCCAGGCT CAACTGGTCT AAGAGGGTTC 
ATTGGTTTTC CAGGACTTCC AGGTGACCAG GGTGAGCCAG GTTCTCCAGG TCCCCCTGGA 
TTTTCAGGAA TTGATGGAGC AAGAGGACCT AAAGGAAACA AAGGTGACCC TGCCAGTCAC 
TTTGGTCCAC CTGGTCCAAA GGGTGAGCCA GGTAGCCCTG GATGTCCAGG GCATTTTGGA 
GCATCTGGAG AGCAGGGCTT GCCTGGTATT CAAGGGCCCA GAGGATCACC TGGAAGGCCA 
GGGCCACCTG GCTCCTCTGG ACCACCAGGG TGCCCAGGTG ATCACGGGAT GCCTGGGCTG 
AGGGGACAGC CAGGAGAAAT GGGAGACCCT GGGCCAAGAG GCCTCCAGGG GGATCCAGGG 
ATACCAGGTC CTCCGGGAAT AAAAGGTCCC TCCGGATCAC CTGGCCTGAA CGGCTTGCAT 
GGATTGAAAG GTCAGAAAGG AACTAAAGGT GCTTCAGGTT TGCATGATGT GGGGCCACCT 
GGTCCAGTGG GAATACCTGG GCTAAAAGGG GAGAGAGGAG ACCCTGGGAG CCCAGGAATC 
TCTCCTCCAG GTCCTCGTGG AGAAAAAGGT CCCCCAGGAC CCCCAGGGAG TTCAGGACCA 
CCTGGTCCTG CAGGTGCCAC AGGAAGAGCT CCTAAGGACA TTCCTGACCC GGGTCCACCT 
GGAGATCAGG GACCTCCTGG TCCTGATGGC CCAAGAGGAG CACCTGGGCC TCCAGGCCTC 
CCTGGGAGTG TTGACCTTCT GAGAGGGGAG CCAGGTGACT GTGGTCTACC AGGGCCACCA 
GGTCCCCCTG GCCCACCAGG CCCTCCAGGA TACAAAGGCT TTCCAGGATG TGATGGAAAA 
GATGGCCAGA AAGGACCAAT GGGATTCCCG GGACCGCAGG GACCACATGG ATTTCCTGGG 
CCACCTGGAG AGAAGGGTTT ACCTGGACCT CCAGGGAGAA AAGGGCCCAC TGGTCTTCCA 
GGTCCCAGAG GTGAACCGGG GCCACCTGCA GATGTGGATG ACTGTCCCCG AATCCCAGGC 
CTTCCTGGGG CGCCAGGCAT GAGAGGACCA GAAGGAGCCA TGGGGCTCTC TGGAATGAGA 
GGCCCCCCAG GACCAGCTCA TGGGTGTGTT CTGAGGACCA AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL4A4 ( XM_016950595.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016806084.2 Pan troglodytes collagen type IV alpha 4 chain (COL4A4), transcript variant X15, mRNA.

Price & Availability↑

CloneID	OPc104276
Clone ID Related Accession (Same CDS sequence)	XM_016950594.2 , XM_016950595.2 , XM_016950596.2 , XM_009444472.3
Accession Version	XM_016950595.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4245bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	collagen alpha-4(IV) chain isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036881.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_016950595.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGTCTC TGCACATAGT ACTAATGAGG TGCTCCTTCA GATTGACCAA GTCCTTGGCC 
ACAGATCCCT GGTCACTTAC ACTCATTCTC TTTTCTGTAC AATATGTATA TGGGAGTGGA 
AAGAAATACG TTGGTCCTTG TGGAGGAAGA GATTGCTCTG TTTGCCACTG TGTTCCTGAA 
AAGGGGTCTC GGGGTCCACC AGGACCACCA GGGCCACAGG GTCCAATTGG ACCCCTGGGA 
GCCCCAGGAC CCATTGGGCT TTCAGGAGAG AAAGGAATGA GAGGGGACCG CGGCCCTCCT 
GGAGCAGCAG GGGACAAAGG AGATAAGGGT TCAACTGGTG TTCCTGGATT TCCAGGTTTA 
GATGGCATAC CTGGGCACCC AGGGCCTCCT GGACCCAGAG GCAAACCTGG TATGAGTGGC 
CACAATGGCT CAAGAGGTGA CCCAGGGTTT CCAGGAGGAA GAGGAGCTCT TGGCCCAGGA 
GGCCCCCTAG GCCATCCTGG GGAAAAGGGA GAAAAAGGAA ATTCAGTGTT CATTTTAGGT 
GCCGTTAAAG GTATTCAGGG AGACAGAGGG GACCCAGGAC TGCCTGGCTT ACCAGGATCT 
TGGGGGGCAG GAGGACCGGC GGGTCCCACA GGATATCCTG GAGAGCCAGG GTTAGTGGGA 
CCTCCGGGCC AACCAGGGCG TCCAGGTTTG AAGGGAAATC CCGGTGTGGG AGTAAAGGGG 
CAAATGGGAG ACCCGGGTGA GGTTGGTCAG CAAGGTTCTC CTGGACCCAC CCTGTTGGTG 
GAGCCACCTG ACTTTCGTCT CTATAAAGGA GAAAAGGGTA TAAAAGGAAT TCCTGGAATG 
GTTGGACTGC CAGGACCACC AGGACGCAAG GGAGAATCTG ATATTGGGGC AAAAGGAGAA 
AAAGGTATTC CTGGATTTCC AGGGCCTCGG GGGGATCCTG GTTCCTATGG ATCTCCAGGT 
TTTCCAGGAT TAAAGGGAGA ACTAGGACTG GTTGGAGATC CTGGGCTATT TGGATTAATT 
GGCCCAAAGG GGGATCCTGG AAATCGAGGG CACCCAGGAC CACCAGGTGT TTTGGTGACT 
CCACCTCTTC CACTCAAAGG CCCACCAGGG GACCCAGGGT TCCCTGGCCG CTATGGAGAA 
ACAGGGGATG TTGGACCACC TGGTCCCCCA GGTCTCTTGG GCAGACCAGG GGAAGCCTGT 
GCAGGCATGA TAGGACCCCC TGGGCCACAA GGATTTCCTG GTCTTCCTGG GCTTCCAGGA 
GAAGCTGGTA TTCCTGGGAG ACCTGATTCT GCTCCAGGAA AACCAGGGAA GCCAGGATCA 
CCTGGCTTGC CTGGAGCACC AGGCCTGCAG GGCCTCCCAG GATCAAGTGT GATATACTGT 
AGTGTTGGGA ATCCTGGACC ACAAGGAATA AAAGGCAAAG TGGGTCCCCC AGGAGGAAGA 
GGCTCAAAAG GAGAAAAAGG AAATGAAGGA CTCTGTGCCT GTGAGCCTGG ACCCATGGGC 
CCCCCTGGCC CTCCAGGACT TCCTGGGAGG CAGGGGAGTA AGGGAGACTT GGGGCTCCCT 
GGCTGGCTTG GAACAAAAGG TGACCCAGGA CCTCCTGGTG CTGAAGGACC TCCAGGGCTA 
CCAGGAAAGC ATGGTGCCTC TGGACCACCT GGCAACAAAG GGGCGAAGGG TGACATGGTT 
GTATCAAGAG TTAAAGGGCA CAAAGGAGAA AGAGGTCCTG ATGGGCCCCC AGGATTTCCA 
GGGCAGCCAG GATCACATGG TCGGGATGGA CATGCTGGAG AAAAAGGGGA TCCAGGACCC 
CCAGGGGATC ATGAAGATGC GACCCCAGGT GGTAAAGGAT TTCCTGGACC TCTGGGCCCC 
CCGGGCAAAG CAGGACCTTT GGGGCCCCCA GGACTGGGAT TTCCTGGTCC ACCAGGACAG 
CGAGGCCACC CAGGAGTTCC AGGCCACCCA GGTGTGAGGG GCCCTGATGG CTTGAAGGGT 
CAGAAAGGTG ACACAATTTC TTGCAACGTA ACCTACCCTG GGAGGCACGG CCCTCCAGGT 
TTTGATGGAC CTCCAGGTCC GAAGGGATTT CCAGGTCCCC AAGGTGCCCC TGGGCTGAGT 
GGTTCAGATG GGCATAAAGG CAGACCTGGC ACACCAGGAA CAGCGGAAAT ACCAGGTCCA 
CCTGGTTTTC GTGGTGACAT GGGAGATCCG GGTTTTGGAG GTGAAAAGGG GTCCTCCCCT 
GCTGGGCCCC CAGGCCCTCC CGGCTCACCA GGAGTGAATG GTCAGAAAGG AATCCCGGGA 
GACCCTGCAT TTGGTCACCT GGGACCCCCG GGAAAGAGGG GTCTTTCAGG AGTGCCAGGG 
ATAAAAGGAC CCAGAGGTGA TCCGGGATGT CCAGGGGCTG AAGGGCCAGC TGGCATTCCT 
GGATTCCCAG GTCTCAAAGG TCCCAAAGGC AGAGAGGGAC ATGCTGGGTT TCCAGGTGTC 
CCAGGTCCAC CTGGCCATTC CTGTGAAAGA GGTGCTCCAG GGATACCAGG GCAACCGGGA 
CTCCCTGGGT ATCCAGGTAG CCCAGGTGCT CCAGGTGGGA AAGGACAGCT GGGAGATGTG 
GGGCCTCCCG GGCCAGCTGG AATGAAAGGC CTCCCTGGAC TCCCAGGATG GCCTGGGGCA 
CATGGTCCCC CAGGCCTCCC AGGAATCCCA GGTCCCTTTG GAGATGATGG GCTACCTGGT 
CCTCCAGGTC CAAAGGGACC CCAGGGGCTG CCTGGTTTCC CAGGTTTTCC CGGAGAAAGA 
GGAAAGCCTG GTGCAGAGGG ATGTCCTGGC ACAAAGGGAG AACCTGGAGA GAAGGGCATG 
TCTGGCCTTC CCGGAGACCG GGGACTGAGA GGGGCCAAAG GAGCCATAGG ACCTCCCGGA 
GATGAAGGAG AAATGGCTAT CATTTCCCAA AAGGGAACAC CTGGGGAACC TGGACCTCCT 
GGAGATGATG GATTCCCAGG AGAAAGAGGT GATAAAGGAA CTCCCGGGAT GCAAGGGAGA 
AGAGGAGAGC CGGGAAGATA CGGACCACCT GGATTTCAGA GAGGGGAACC TGGTGAGAAA 
GGTCAGCCAG GGCATCCTGG ACCCCCAGGC CCTCCAGGCT CAACTGGTCT AAGAGGGTTC 
ATTGGTTTTC CAGGACTTCC AGGTGACCAG GGTGAGCCAG GTTCTCCAGG TCCCCCTGGA 
TTTTCAGGAA TTGATGGAGC AAGAGGACCT AAAGGAAACA AAGGTGACCC TGCCAGTCAC 
TTTGGTCCAC CTGGTCCAAA GGGTGAGCCA GGTAGCCCTG GATGTCCAGG GCATTTTGGA 
GCATCTGGAG AGCAGGGCTT GCCTGGTATT CAAGGGCCCA GAGGATCACC TGGAAGGCCA 
GGGCCACCTG GCTCCTCTGG ACCACCAGGG TGCCCAGGTG ATCACGGGAT GCCTGGGCTG 
AGGGGACAGC CAGGAGAAAT GGGAGACCCT GGGCCAAGAG GCCTCCAGGG GGATCCAGGG 
ATACCAGGTC CTCCGGGAAT AAAAGGTCCC TCCGGATCAC CTGGCCTGAA CGGCTTGCAT 
GGATTGAAAG GTCAGAAAGG AACTAAAGGT GCTTCAGGTT TGCATGATGT GGGGCCACCT 
GGTCCAGTGG GAATACCTGG GCTAAAAGGG GAGAGAGGAG ACCCTGGGAG CCCAGGAATC 
TCTCCTCCAG GTCCTCGTGG AGAAAAAGGT CCCCCAGGAC CCCCAGGGAG TTCAGGACCA 
CCTGGTCCTG CAGGTGCCAC AGGAAGAGCT CCTAAGGACA TTCCTGACCC GGGTCCACCT 
GGAGATCAGG GACCTCCTGG TCCTGATGGC CCAAGAGGAG CACCTGGGCC TCCAGGCCTC 
CCTGGGAGTG TTGACCTTCT GAGAGGGGAG CCAGGTGACT GTGGTCTACC AGGGCCACCA 
GGTCCCCCTG GCCCACCAGG CCCTCCAGGA TACAAAGGCT TTCCAGGATG TGATGGAAAA 
GATGGCCAGA AAGGACCAAT GGGATTCCCG GGACCGCAGG GACCACATGG ATTTCCTGGG 
CCACCTGGAG AGAAGGGTTT ACCTGGACCT CCAGGGAGAA AAGGGCCCAC TGGTCTTCCA 
GGTCCCAGAG GTGAACCGGG GCCACCTGCA GATGTGGATG ACTGTCCCCG AATCCCAGGC 
CTTCCTGGGG CGCCAGGCAT GAGAGGACCA GAAGGAGCCA TGGGGCTCTC TGGAATGAGA 
GGCCCCCCAG GACCAGCTCA TGGGTGTGTT CTGAGGACCA AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016806084.2
CDS	422..4666
Translation	MWSLHIVLMRCSFRLTKSLATDPWSLTLILFSVQYVYGSGKKYVGPCGGRDCSVCHCVPEKGSRGPPGPPGPQGPIGPLGAPGPIGLSGEKGMRGDRGPPGAAGDKGDKGSTGVPGFPGLDGIPGHPGPPGPRGKPGMSGHNGSRGDPGFPGGRGALGPGGPLGHPGEKGEKGNSVFILGAVKGIQGDRGDPGLPGLPGSWGAGGPAGPTGYPGEPGLVGPPGQPGRPGLKGNPGVGVKGQMGDPGEVGQQGSPGPTLLVEPPDFRLYKGEKGIKGIPGMVGLPGPPGRKGESDIGAKGEKGIPGFPGPRGDPGSYGSPGFPGLKGELGLVGDPGLFGLIGPKGDPGNRGHPGPPGVLVTPPLPLKGPPGDPGFPGRYGETGDVGPPGPPGLLGRPGEACAGMIGPPGPQGFPGLPGLPGEAGIPGRPDSAPGKPGKPGSPGLPGAPGLQGLPGSSVIYCSVGNPGPQGIKGKVGPPGGRGSKGEKGNEGLCACEPGPMGPPGPPGLPGRQGSKGDLGLPGWLGTKGDPGPPGAEGPPGLPGKHGASGPPGNKGAKGDMVVSRVKGHKGERGPDGPPGFPGQPGSHGRDGHAGEKGDPGPPGDHEDATPGGKGFPGPLGPPGKAGPLGPPGLGFPGPPGQRGHPGVPGHPGVRGPDGLKGQKGDTISCNVTYPGRHGPPGFDGPPGPKGFPGPQGAPGLSGSDGHKGRPGTPGTAEIPGPPGFRGDMGDPGFGGEKGSSPAGPPGPPGSPGVNGQKGIPGDPAFGHLGPPGKRGLSGVPGIKGPRGDPGCPGAEGPAGIPGFPGLKGPKGREGHAGFPGVPGPPGHSCERGAPGIPGQPGLPGYPGSPGAPGGKGQLGDVGPPGPAGMKGLPGLPGWPGAHGPPGLPGIPGPFGDDGLPGPPGPKGPQGLPGFPGFPGERGKPGAEGCPGTKGEPGEKGMSGLPGDRGLRGAKGAIGPPGDEGEMAIISQKGTPGEPGPPGDDGFPGERGDKGTPGMQGRRGEPGRYGPPGFQRGEPGEKGQPGHPGPPGPPGSTGLRGFIGFPGLPGDQGEPGSPGPPGFSGIDGARGPKGNKGDPASHFGPPGPKGEPGSPGCPGHFGASGEQGLPGIQGPRGSPGRPGPPGSSGPPGCPGDHGMPGLRGQPGEMGDPGPRGLQGDPGIPGPPGIKGPSGSPGLNGLHGLKGQKGTKGASGLHDVGPPGPVGIPGLKGERGDPGSPGISPPGPRGEKGPPGPPGSSGPPGPAGATGRAPKDIPDPGPPGDQGPPGPDGPRGAPGPPGLPGSVDLLRGEPGDCGLPGPPGPPGPPGPPGYKGFPGCDGKDGQKGPMGFPGPQGPHGFPGPPGEKGLPGPPGRKGPTGLPGPRGEPGPPADVDDCPRIPGLPGAPGMRGPEGAMGLSGMRGPPGPAHGCVLRTK

Target ORF information:

RefSeq Version	XM_016950595.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes collagen type IV alpha 4 chain (COL4A4), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016950595.2

ORF Insert Sequence:

ATGTGGTCTC TGCACATAGT ACTAATGAGG TGCTCCTTCA GATTGACCAA GTCCTTGGCC 
ACAGATCCCT GGTCACTTAC ACTCATTCTC TTTTCTGTAC AATATGTATA TGGGAGTGGA 
AAGAAATACG TTGGTCCTTG TGGAGGAAGA GATTGCTCTG TTTGCCACTG TGTTCCTGAA 
AAGGGGTCTC GGGGTCCACC AGGACCACCA GGGCCACAGG GTCCAATTGG ACCCCTGGGA 
GCCCCAGGAC CCATTGGGCT TTCAGGAGAG AAAGGAATGA GAGGGGACCG CGGCCCTCCT 
GGAGCAGCAG GGGACAAAGG AGATAAGGGT TCAACTGGTG TTCCTGGATT TCCAGGTTTA 
GATGGCATAC CTGGGCACCC AGGGCCTCCT GGACCCAGAG GCAAACCTGG TATGAGTGGC 
CACAATGGCT CAAGAGGTGA CCCAGGGTTT CCAGGAGGAA GAGGAGCTCT TGGCCCAGGA 
GGCCCCCTAG GCCATCCTGG GGAAAAGGGA GAAAAAGGAA ATTCAGTGTT CATTTTAGGT 
GCCGTTAAAG GTATTCAGGG AGACAGAGGG GACCCAGGAC TGCCTGGCTT ACCAGGATCT 
TGGGGGGCAG GAGGACCGGC GGGTCCCACA GGATATCCTG GAGAGCCAGG GTTAGTGGGA 
CCTCCGGGCC AACCAGGGCG TCCAGGTTTG AAGGGAAATC CCGGTGTGGG AGTAAAGGGG 
CAAATGGGAG ACCCGGGTGA GGTTGGTCAG CAAGGTTCTC CTGGACCCAC CCTGTTGGTG 
GAGCCACCTG ACTTTCGTCT CTATAAAGGA GAAAAGGGTA TAAAAGGAAT TCCTGGAATG 
GTTGGACTGC CAGGACCACC AGGACGCAAG GGAGAATCTG ATATTGGGGC AAAAGGAGAA 
AAAGGTATTC CTGGATTTCC AGGGCCTCGG GGGGATCCTG GTTCCTATGG ATCTCCAGGT 
TTTCCAGGAT TAAAGGGAGA ACTAGGACTG GTTGGAGATC CTGGGCTATT TGGATTAATT 
GGCCCAAAGG GGGATCCTGG AAATCGAGGG CACCCAGGAC CACCAGGTGT TTTGGTGACT 
CCACCTCTTC CACTCAAAGG CCCACCAGGG GACCCAGGGT TCCCTGGCCG CTATGGAGAA 
ACAGGGGATG TTGGACCACC TGGTCCCCCA GGTCTCTTGG GCAGACCAGG GGAAGCCTGT 
GCAGGCATGA TAGGACCCCC TGGGCCACAA GGATTTCCTG GTCTTCCTGG GCTTCCAGGA 
GAAGCTGGTA TTCCTGGGAG ACCTGATTCT GCTCCAGGAA AACCAGGGAA GCCAGGATCA 
CCTGGCTTGC CTGGAGCACC AGGCCTGCAG GGCCTCCCAG GATCAAGTGT GATATACTGT 
AGTGTTGGGA ATCCTGGACC ACAAGGAATA AAAGGCAAAG TGGGTCCCCC AGGAGGAAGA 
GGCTCAAAAG GAGAAAAAGG AAATGAAGGA CTCTGTGCCT GTGAGCCTGG ACCCATGGGC 
CCCCCTGGCC CTCCAGGACT TCCTGGGAGG CAGGGGAGTA AGGGAGACTT GGGGCTCCCT 
GGCTGGCTTG GAACAAAAGG TGACCCAGGA CCTCCTGGTG CTGAAGGACC TCCAGGGCTA 
CCAGGAAAGC ATGGTGCCTC TGGACCACCT GGCAACAAAG GGGCGAAGGG TGACATGGTT 
GTATCAAGAG TTAAAGGGCA CAAAGGAGAA AGAGGTCCTG ATGGGCCCCC AGGATTTCCA 
GGGCAGCCAG GATCACATGG TCGGGATGGA CATGCTGGAG AAAAAGGGGA TCCAGGACCC 
CCAGGGGATC ATGAAGATGC GACCCCAGGT GGTAAAGGAT TTCCTGGACC TCTGGGCCCC 
CCGGGCAAAG CAGGACCTTT GGGGCCCCCA GGACTGGGAT TTCCTGGTCC ACCAGGACAG 
CGAGGCCACC CAGGAGTTCC AGGCCACCCA GGTGTGAGGG GCCCTGATGG CTTGAAGGGT 
CAGAAAGGTG ACACAATTTC TTGCAACGTA ACCTACCCTG GGAGGCACGG CCCTCCAGGT 
TTTGATGGAC CTCCAGGTCC GAAGGGATTT CCAGGTCCCC AAGGTGCCCC TGGGCTGAGT 
GGTTCAGATG GGCATAAAGG CAGACCTGGC ACACCAGGAA CAGCGGAAAT ACCAGGTCCA 
CCTGGTTTTC GTGGTGACAT GGGAGATCCG GGTTTTGGAG GTGAAAAGGG GTCCTCCCCT 
GCTGGGCCCC CAGGCCCTCC CGGCTCACCA GGAGTGAATG GTCAGAAAGG AATCCCGGGA 
GACCCTGCAT TTGGTCACCT GGGACCCCCG GGAAAGAGGG GTCTTTCAGG AGTGCCAGGG 
ATAAAAGGAC CCAGAGGTGA TCCGGGATGT CCAGGGGCTG AAGGGCCAGC TGGCATTCCT 
GGATTCCCAG GTCTCAAAGG TCCCAAAGGC AGAGAGGGAC ATGCTGGGTT TCCAGGTGTC 
CCAGGTCCAC CTGGCCATTC CTGTGAAAGA GGTGCTCCAG GGATACCAGG GCAACCGGGA 
CTCCCTGGGT ATCCAGGTAG CCCAGGTGCT CCAGGTGGGA AAGGACAGCT GGGAGATGTG 
GGGCCTCCCG GGCCAGCTGG AATGAAAGGC CTCCCTGGAC TCCCAGGATG GCCTGGGGCA 
CATGGTCCCC CAGGCCTCCC AGGAATCCCA GGTCCCTTTG GAGATGATGG GCTACCTGGT 
CCTCCAGGTC CAAAGGGACC CCAGGGGCTG CCTGGTTTCC CAGGTTTTCC CGGAGAAAGA 
GGAAAGCCTG GTGCAGAGGG ATGTCCTGGC ACAAAGGGAG AACCTGGAGA GAAGGGCATG 
TCTGGCCTTC CCGGAGACCG GGGACTGAGA GGGGCCAAAG GAGCCATAGG ACCTCCCGGA 
GATGAAGGAG AAATGGCTAT CATTTCCCAA AAGGGAACAC CTGGGGAACC TGGACCTCCT 
GGAGATGATG GATTCCCAGG AGAAAGAGGT GATAAAGGAA CTCCCGGGAT GCAAGGGAGA 
AGAGGAGAGC CGGGAAGATA CGGACCACCT GGATTTCAGA GAGGGGAACC TGGTGAGAAA 
GGTCAGCCAG GGCATCCTGG ACCCCCAGGC CCTCCAGGCT CAACTGGTCT AAGAGGGTTC 
ATTGGTTTTC CAGGACTTCC AGGTGACCAG GGTGAGCCAG GTTCTCCAGG TCCCCCTGGA 
TTTTCAGGAA TTGATGGAGC AAGAGGACCT AAAGGAAACA AAGGTGACCC TGCCAGTCAC 
TTTGGTCCAC CTGGTCCAAA GGGTGAGCCA GGTAGCCCTG GATGTCCAGG GCATTTTGGA 
GCATCTGGAG AGCAGGGCTT GCCTGGTATT CAAGGGCCCA GAGGATCACC TGGAAGGCCA 
GGGCCACCTG GCTCCTCTGG ACCACCAGGG TGCCCAGGTG ATCACGGGAT GCCTGGGCTG 
AGGGGACAGC CAGGAGAAAT GGGAGACCCT GGGCCAAGAG GCCTCCAGGG GGATCCAGGG 
ATACCAGGTC CTCCGGGAAT AAAAGGTCCC TCCGGATCAC CTGGCCTGAA CGGCTTGCAT 
GGATTGAAAG GTCAGAAAGG AACTAAAGGT GCTTCAGGTT TGCATGATGT GGGGCCACCT 
GGTCCAGTGG GAATACCTGG GCTAAAAGGG GAGAGAGGAG ACCCTGGGAG CCCAGGAATC 
TCTCCTCCAG GTCCTCGTGG AGAAAAAGGT CCCCCAGGAC CCCCAGGGAG TTCAGGACCA 
CCTGGTCCTG CAGGTGCCAC AGGAAGAGCT CCTAAGGACA TTCCTGACCC GGGTCCACCT 
GGAGATCAGG GACCTCCTGG TCCTGATGGC CCAAGAGGAG CACCTGGGCC TCCAGGCCTC 
CCTGGGAGTG TTGACCTTCT GAGAGGGGAG CCAGGTGACT GTGGTCTACC AGGGCCACCA 
GGTCCCCCTG GCCCACCAGG CCCTCCAGGA TACAAAGGCT TTCCAGGATG TGATGGAAAA 
GATGGCCAGA AAGGACCAAT GGGATTCCCG GGACCGCAGG GACCACATGG ATTTCCTGGG 
CCACCTGGAG AGAAGGGTTT ACCTGGACCT CCAGGGAGAA AAGGGCCCAC TGGTCTTCCA 
GGTCCCAGAG GTGAACCGGG GCCACCTGCA GATGTGGATG ACTGTCCCCG AATCCCAGGC 
CTTCCTGGGG CGCCAGGCAT GAGAGGACCA GAAGGAGCCA TGGGGCTCTC TGGAATGAGA 
GGCCCCCCAG GACCAGCTCA TGGGTGTGTT CTGAGGACCA AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL4A4 ( XM_016950596.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016806085.2 Pan troglodytes collagen type IV alpha 4 chain (COL4A4), transcript variant X16, mRNA.

Price & Availability↑

CloneID	OPc104276
Clone ID Related Accession (Same CDS sequence)	XM_016950594.2 , XM_016950595.2 , XM_016950596.2 , XM_009444472.3
Accession Version	XM_016950596.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4245bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	collagen alpha-4(IV) chain isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036881.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_016950596.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGTCTC TGCACATAGT ACTAATGAGG TGCTCCTTCA GATTGACCAA GTCCTTGGCC 
ACAGATCCCT GGTCACTTAC ACTCATTCTC TTTTCTGTAC AATATGTATA TGGGAGTGGA 
AAGAAATACG TTGGTCCTTG TGGAGGAAGA GATTGCTCTG TTTGCCACTG TGTTCCTGAA 
AAGGGGTCTC GGGGTCCACC AGGACCACCA GGGCCACAGG GTCCAATTGG ACCCCTGGGA 
GCCCCAGGAC CCATTGGGCT TTCAGGAGAG AAAGGAATGA GAGGGGACCG CGGCCCTCCT 
GGAGCAGCAG GGGACAAAGG AGATAAGGGT TCAACTGGTG TTCCTGGATT TCCAGGTTTA 
GATGGCATAC CTGGGCACCC AGGGCCTCCT GGACCCAGAG GCAAACCTGG TATGAGTGGC 
CACAATGGCT CAAGAGGTGA CCCAGGGTTT CCAGGAGGAA GAGGAGCTCT TGGCCCAGGA 
GGCCCCCTAG GCCATCCTGG GGAAAAGGGA GAAAAAGGAA ATTCAGTGTT CATTTTAGGT 
GCCGTTAAAG GTATTCAGGG AGACAGAGGG GACCCAGGAC TGCCTGGCTT ACCAGGATCT 
TGGGGGGCAG GAGGACCGGC GGGTCCCACA GGATATCCTG GAGAGCCAGG GTTAGTGGGA 
CCTCCGGGCC AACCAGGGCG TCCAGGTTTG AAGGGAAATC CCGGTGTGGG AGTAAAGGGG 
CAAATGGGAG ACCCGGGTGA GGTTGGTCAG CAAGGTTCTC CTGGACCCAC CCTGTTGGTG 
GAGCCACCTG ACTTTCGTCT CTATAAAGGA GAAAAGGGTA TAAAAGGAAT TCCTGGAATG 
GTTGGACTGC CAGGACCACC AGGACGCAAG GGAGAATCTG ATATTGGGGC AAAAGGAGAA 
AAAGGTATTC CTGGATTTCC AGGGCCTCGG GGGGATCCTG GTTCCTATGG ATCTCCAGGT 
TTTCCAGGAT TAAAGGGAGA ACTAGGACTG GTTGGAGATC CTGGGCTATT TGGATTAATT 
GGCCCAAAGG GGGATCCTGG AAATCGAGGG CACCCAGGAC CACCAGGTGT TTTGGTGACT 
CCACCTCTTC CACTCAAAGG CCCACCAGGG GACCCAGGGT TCCCTGGCCG CTATGGAGAA 
ACAGGGGATG TTGGACCACC TGGTCCCCCA GGTCTCTTGG GCAGACCAGG GGAAGCCTGT 
GCAGGCATGA TAGGACCCCC TGGGCCACAA GGATTTCCTG GTCTTCCTGG GCTTCCAGGA 
GAAGCTGGTA TTCCTGGGAG ACCTGATTCT GCTCCAGGAA AACCAGGGAA GCCAGGATCA 
CCTGGCTTGC CTGGAGCACC AGGCCTGCAG GGCCTCCCAG GATCAAGTGT GATATACTGT 
AGTGTTGGGA ATCCTGGACC ACAAGGAATA AAAGGCAAAG TGGGTCCCCC AGGAGGAAGA 
GGCTCAAAAG GAGAAAAAGG AAATGAAGGA CTCTGTGCCT GTGAGCCTGG ACCCATGGGC 
CCCCCTGGCC CTCCAGGACT TCCTGGGAGG CAGGGGAGTA AGGGAGACTT GGGGCTCCCT 
GGCTGGCTTG GAACAAAAGG TGACCCAGGA CCTCCTGGTG CTGAAGGACC TCCAGGGCTA 
CCAGGAAAGC ATGGTGCCTC TGGACCACCT GGCAACAAAG GGGCGAAGGG TGACATGGTT 
GTATCAAGAG TTAAAGGGCA CAAAGGAGAA AGAGGTCCTG ATGGGCCCCC AGGATTTCCA 
GGGCAGCCAG GATCACATGG TCGGGATGGA CATGCTGGAG AAAAAGGGGA TCCAGGACCC 
CCAGGGGATC ATGAAGATGC GACCCCAGGT GGTAAAGGAT TTCCTGGACC TCTGGGCCCC 
CCGGGCAAAG CAGGACCTTT GGGGCCCCCA GGACTGGGAT TTCCTGGTCC ACCAGGACAG 
CGAGGCCACC CAGGAGTTCC AGGCCACCCA GGTGTGAGGG GCCCTGATGG CTTGAAGGGT 
CAGAAAGGTG ACACAATTTC TTGCAACGTA ACCTACCCTG GGAGGCACGG CCCTCCAGGT 
TTTGATGGAC CTCCAGGTCC GAAGGGATTT CCAGGTCCCC AAGGTGCCCC TGGGCTGAGT 
GGTTCAGATG GGCATAAAGG CAGACCTGGC ACACCAGGAA CAGCGGAAAT ACCAGGTCCA 
CCTGGTTTTC GTGGTGACAT GGGAGATCCG GGTTTTGGAG GTGAAAAGGG GTCCTCCCCT 
GCTGGGCCCC CAGGCCCTCC CGGCTCACCA GGAGTGAATG GTCAGAAAGG AATCCCGGGA 
GACCCTGCAT TTGGTCACCT GGGACCCCCG GGAAAGAGGG GTCTTTCAGG AGTGCCAGGG 
ATAAAAGGAC CCAGAGGTGA TCCGGGATGT CCAGGGGCTG AAGGGCCAGC TGGCATTCCT 
GGATTCCCAG GTCTCAAAGG TCCCAAAGGC AGAGAGGGAC ATGCTGGGTT TCCAGGTGTC 
CCAGGTCCAC CTGGCCATTC CTGTGAAAGA GGTGCTCCAG GGATACCAGG GCAACCGGGA 
CTCCCTGGGT ATCCAGGTAG CCCAGGTGCT CCAGGTGGGA AAGGACAGCT GGGAGATGTG 
GGGCCTCCCG GGCCAGCTGG AATGAAAGGC CTCCCTGGAC TCCCAGGATG GCCTGGGGCA 
CATGGTCCCC CAGGCCTCCC AGGAATCCCA GGTCCCTTTG GAGATGATGG GCTACCTGGT 
CCTCCAGGTC CAAAGGGACC CCAGGGGCTG CCTGGTTTCC CAGGTTTTCC CGGAGAAAGA 
GGAAAGCCTG GTGCAGAGGG ATGTCCTGGC ACAAAGGGAG AACCTGGAGA GAAGGGCATG 
TCTGGCCTTC CCGGAGACCG GGGACTGAGA GGGGCCAAAG GAGCCATAGG ACCTCCCGGA 
GATGAAGGAG AAATGGCTAT CATTTCCCAA AAGGGAACAC CTGGGGAACC TGGACCTCCT 
GGAGATGATG GATTCCCAGG AGAAAGAGGT GATAAAGGAA CTCCCGGGAT GCAAGGGAGA 
AGAGGAGAGC CGGGAAGATA CGGACCACCT GGATTTCAGA GAGGGGAACC TGGTGAGAAA 
GGTCAGCCAG GGCATCCTGG ACCCCCAGGC CCTCCAGGCT CAACTGGTCT AAGAGGGTTC 
ATTGGTTTTC CAGGACTTCC AGGTGACCAG GGTGAGCCAG GTTCTCCAGG TCCCCCTGGA 
TTTTCAGGAA TTGATGGAGC AAGAGGACCT AAAGGAAACA AAGGTGACCC TGCCAGTCAC 
TTTGGTCCAC CTGGTCCAAA GGGTGAGCCA GGTAGCCCTG GATGTCCAGG GCATTTTGGA 
GCATCTGGAG AGCAGGGCTT GCCTGGTATT CAAGGGCCCA GAGGATCACC TGGAAGGCCA 
GGGCCACCTG GCTCCTCTGG ACCACCAGGG TGCCCAGGTG ATCACGGGAT GCCTGGGCTG 
AGGGGACAGC CAGGAGAAAT GGGAGACCCT GGGCCAAGAG GCCTCCAGGG GGATCCAGGG 
ATACCAGGTC CTCCGGGAAT AAAAGGTCCC TCCGGATCAC CTGGCCTGAA CGGCTTGCAT 
GGATTGAAAG GTCAGAAAGG AACTAAAGGT GCTTCAGGTT TGCATGATGT GGGGCCACCT 
GGTCCAGTGG GAATACCTGG GCTAAAAGGG GAGAGAGGAG ACCCTGGGAG CCCAGGAATC 
TCTCCTCCAG GTCCTCGTGG AGAAAAAGGT CCCCCAGGAC CCCCAGGGAG TTCAGGACCA 
CCTGGTCCTG CAGGTGCCAC AGGAAGAGCT CCTAAGGACA TTCCTGACCC GGGTCCACCT 
GGAGATCAGG GACCTCCTGG TCCTGATGGC CCAAGAGGAG CACCTGGGCC TCCAGGCCTC 
CCTGGGAGTG TTGACCTTCT GAGAGGGGAG CCAGGTGACT GTGGTCTACC AGGGCCACCA 
GGTCCCCCTG GCCCACCAGG CCCTCCAGGA TACAAAGGCT TTCCAGGATG TGATGGAAAA 
GATGGCCAGA AAGGACCAAT GGGATTCCCG GGACCGCAGG GACCACATGG ATTTCCTGGG 
CCACCTGGAG AGAAGGGTTT ACCTGGACCT CCAGGGAGAA AAGGGCCCAC TGGTCTTCCA 
GGTCCCAGAG GTGAACCGGG GCCACCTGCA GATGTGGATG ACTGTCCCCG AATCCCAGGC 
CTTCCTGGGG CGCCAGGCAT GAGAGGACCA GAAGGAGCCA TGGGGCTCTC TGGAATGAGA 
GGCCCCCCAG GACCAGCTCA TGGGTGTGTT CTGAGGACCA AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016806085.2
CDS	422..4666
Translation	MWSLHIVLMRCSFRLTKSLATDPWSLTLILFSVQYVYGSGKKYVGPCGGRDCSVCHCVPEKGSRGPPGPPGPQGPIGPLGAPGPIGLSGEKGMRGDRGPPGAAGDKGDKGSTGVPGFPGLDGIPGHPGPPGPRGKPGMSGHNGSRGDPGFPGGRGALGPGGPLGHPGEKGEKGNSVFILGAVKGIQGDRGDPGLPGLPGSWGAGGPAGPTGYPGEPGLVGPPGQPGRPGLKGNPGVGVKGQMGDPGEVGQQGSPGPTLLVEPPDFRLYKGEKGIKGIPGMVGLPGPPGRKGESDIGAKGEKGIPGFPGPRGDPGSYGSPGFPGLKGELGLVGDPGLFGLIGPKGDPGNRGHPGPPGVLVTPPLPLKGPPGDPGFPGRYGETGDVGPPGPPGLLGRPGEACAGMIGPPGPQGFPGLPGLPGEAGIPGRPDSAPGKPGKPGSPGLPGAPGLQGLPGSSVIYCSVGNPGPQGIKGKVGPPGGRGSKGEKGNEGLCACEPGPMGPPGPPGLPGRQGSKGDLGLPGWLGTKGDPGPPGAEGPPGLPGKHGASGPPGNKGAKGDMVVSRVKGHKGERGPDGPPGFPGQPGSHGRDGHAGEKGDPGPPGDHEDATPGGKGFPGPLGPPGKAGPLGPPGLGFPGPPGQRGHPGVPGHPGVRGPDGLKGQKGDTISCNVTYPGRHGPPGFDGPPGPKGFPGPQGAPGLSGSDGHKGRPGTPGTAEIPGPPGFRGDMGDPGFGGEKGSSPAGPPGPPGSPGVNGQKGIPGDPAFGHLGPPGKRGLSGVPGIKGPRGDPGCPGAEGPAGIPGFPGLKGPKGREGHAGFPGVPGPPGHSCERGAPGIPGQPGLPGYPGSPGAPGGKGQLGDVGPPGPAGMKGLPGLPGWPGAHGPPGLPGIPGPFGDDGLPGPPGPKGPQGLPGFPGFPGERGKPGAEGCPGTKGEPGEKGMSGLPGDRGLRGAKGAIGPPGDEGEMAIISQKGTPGEPGPPGDDGFPGERGDKGTPGMQGRRGEPGRYGPPGFQRGEPGEKGQPGHPGPPGPPGSTGLRGFIGFPGLPGDQGEPGSPGPPGFSGIDGARGPKGNKGDPASHFGPPGPKGEPGSPGCPGHFGASGEQGLPGIQGPRGSPGRPGPPGSSGPPGCPGDHGMPGLRGQPGEMGDPGPRGLQGDPGIPGPPGIKGPSGSPGLNGLHGLKGQKGTKGASGLHDVGPPGPVGIPGLKGERGDPGSPGISPPGPRGEKGPPGPPGSSGPPGPAGATGRAPKDIPDPGPPGDQGPPGPDGPRGAPGPPGLPGSVDLLRGEPGDCGLPGPPGPPGPPGPPGYKGFPGCDGKDGQKGPMGFPGPQGPHGFPGPPGEKGLPGPPGRKGPTGLPGPRGEPGPPADVDDCPRIPGLPGAPGMRGPEGAMGLSGMRGPPGPAHGCVLRTK

Target ORF information:

RefSeq Version	XM_016950596.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes collagen type IV alpha 4 chain (COL4A4), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016950596.2

ORF Insert Sequence:

ATGTGGTCTC TGCACATAGT ACTAATGAGG TGCTCCTTCA GATTGACCAA GTCCTTGGCC 
ACAGATCCCT GGTCACTTAC ACTCATTCTC TTTTCTGTAC AATATGTATA TGGGAGTGGA 
AAGAAATACG TTGGTCCTTG TGGAGGAAGA GATTGCTCTG TTTGCCACTG TGTTCCTGAA 
AAGGGGTCTC GGGGTCCACC AGGACCACCA GGGCCACAGG GTCCAATTGG ACCCCTGGGA 
GCCCCAGGAC CCATTGGGCT TTCAGGAGAG AAAGGAATGA GAGGGGACCG CGGCCCTCCT 
GGAGCAGCAG GGGACAAAGG AGATAAGGGT TCAACTGGTG TTCCTGGATT TCCAGGTTTA 
GATGGCATAC CTGGGCACCC AGGGCCTCCT GGACCCAGAG GCAAACCTGG TATGAGTGGC 
CACAATGGCT CAAGAGGTGA CCCAGGGTTT CCAGGAGGAA GAGGAGCTCT TGGCCCAGGA 
GGCCCCCTAG GCCATCCTGG GGAAAAGGGA GAAAAAGGAA ATTCAGTGTT CATTTTAGGT 
GCCGTTAAAG GTATTCAGGG AGACAGAGGG GACCCAGGAC TGCCTGGCTT ACCAGGATCT 
TGGGGGGCAG GAGGACCGGC GGGTCCCACA GGATATCCTG GAGAGCCAGG GTTAGTGGGA 
CCTCCGGGCC AACCAGGGCG TCCAGGTTTG AAGGGAAATC CCGGTGTGGG AGTAAAGGGG 
CAAATGGGAG ACCCGGGTGA GGTTGGTCAG CAAGGTTCTC CTGGACCCAC CCTGTTGGTG 
GAGCCACCTG ACTTTCGTCT CTATAAAGGA GAAAAGGGTA TAAAAGGAAT TCCTGGAATG 
GTTGGACTGC CAGGACCACC AGGACGCAAG GGAGAATCTG ATATTGGGGC AAAAGGAGAA 
AAAGGTATTC CTGGATTTCC AGGGCCTCGG GGGGATCCTG GTTCCTATGG ATCTCCAGGT 
TTTCCAGGAT TAAAGGGAGA ACTAGGACTG GTTGGAGATC CTGGGCTATT TGGATTAATT 
GGCCCAAAGG GGGATCCTGG AAATCGAGGG CACCCAGGAC CACCAGGTGT TTTGGTGACT 
CCACCTCTTC CACTCAAAGG CCCACCAGGG GACCCAGGGT TCCCTGGCCG CTATGGAGAA 
ACAGGGGATG TTGGACCACC TGGTCCCCCA GGTCTCTTGG GCAGACCAGG GGAAGCCTGT 
GCAGGCATGA TAGGACCCCC TGGGCCACAA GGATTTCCTG GTCTTCCTGG GCTTCCAGGA 
GAAGCTGGTA TTCCTGGGAG ACCTGATTCT GCTCCAGGAA AACCAGGGAA GCCAGGATCA 
CCTGGCTTGC CTGGAGCACC AGGCCTGCAG GGCCTCCCAG GATCAAGTGT GATATACTGT 
AGTGTTGGGA ATCCTGGACC ACAAGGAATA AAAGGCAAAG TGGGTCCCCC AGGAGGAAGA 
GGCTCAAAAG GAGAAAAAGG AAATGAAGGA CTCTGTGCCT GTGAGCCTGG ACCCATGGGC 
CCCCCTGGCC CTCCAGGACT TCCTGGGAGG CAGGGGAGTA AGGGAGACTT GGGGCTCCCT 
GGCTGGCTTG GAACAAAAGG TGACCCAGGA CCTCCTGGTG CTGAAGGACC TCCAGGGCTA 
CCAGGAAAGC ATGGTGCCTC TGGACCACCT GGCAACAAAG GGGCGAAGGG TGACATGGTT 
GTATCAAGAG TTAAAGGGCA CAAAGGAGAA AGAGGTCCTG ATGGGCCCCC AGGATTTCCA 
GGGCAGCCAG GATCACATGG TCGGGATGGA CATGCTGGAG AAAAAGGGGA TCCAGGACCC 
CCAGGGGATC ATGAAGATGC GACCCCAGGT GGTAAAGGAT TTCCTGGACC TCTGGGCCCC 
CCGGGCAAAG CAGGACCTTT GGGGCCCCCA GGACTGGGAT TTCCTGGTCC ACCAGGACAG 
CGAGGCCACC CAGGAGTTCC AGGCCACCCA GGTGTGAGGG GCCCTGATGG CTTGAAGGGT 
CAGAAAGGTG ACACAATTTC TTGCAACGTA ACCTACCCTG GGAGGCACGG CCCTCCAGGT 
TTTGATGGAC CTCCAGGTCC GAAGGGATTT CCAGGTCCCC AAGGTGCCCC TGGGCTGAGT 
GGTTCAGATG GGCATAAAGG CAGACCTGGC ACACCAGGAA CAGCGGAAAT ACCAGGTCCA 
CCTGGTTTTC GTGGTGACAT GGGAGATCCG GGTTTTGGAG GTGAAAAGGG GTCCTCCCCT 
GCTGGGCCCC CAGGCCCTCC CGGCTCACCA GGAGTGAATG GTCAGAAAGG AATCCCGGGA 
GACCCTGCAT TTGGTCACCT GGGACCCCCG GGAAAGAGGG GTCTTTCAGG AGTGCCAGGG 
ATAAAAGGAC CCAGAGGTGA TCCGGGATGT CCAGGGGCTG AAGGGCCAGC TGGCATTCCT 
GGATTCCCAG GTCTCAAAGG TCCCAAAGGC AGAGAGGGAC ATGCTGGGTT TCCAGGTGTC 
CCAGGTCCAC CTGGCCATTC CTGTGAAAGA GGTGCTCCAG GGATACCAGG GCAACCGGGA 
CTCCCTGGGT ATCCAGGTAG CCCAGGTGCT CCAGGTGGGA AAGGACAGCT GGGAGATGTG 
GGGCCTCCCG GGCCAGCTGG AATGAAAGGC CTCCCTGGAC TCCCAGGATG GCCTGGGGCA 
CATGGTCCCC CAGGCCTCCC AGGAATCCCA GGTCCCTTTG GAGATGATGG GCTACCTGGT 
CCTCCAGGTC CAAAGGGACC CCAGGGGCTG CCTGGTTTCC CAGGTTTTCC CGGAGAAAGA 
GGAAAGCCTG GTGCAGAGGG ATGTCCTGGC ACAAAGGGAG AACCTGGAGA GAAGGGCATG 
TCTGGCCTTC CCGGAGACCG GGGACTGAGA GGGGCCAAAG GAGCCATAGG ACCTCCCGGA 
GATGAAGGAG AAATGGCTAT CATTTCCCAA AAGGGAACAC CTGGGGAACC TGGACCTCCT 
GGAGATGATG GATTCCCAGG AGAAAGAGGT GATAAAGGAA CTCCCGGGAT GCAAGGGAGA 
AGAGGAGAGC CGGGAAGATA CGGACCACCT GGATTTCAGA GAGGGGAACC TGGTGAGAAA 
GGTCAGCCAG GGCATCCTGG ACCCCCAGGC CCTCCAGGCT CAACTGGTCT AAGAGGGTTC 
ATTGGTTTTC CAGGACTTCC AGGTGACCAG GGTGAGCCAG GTTCTCCAGG TCCCCCTGGA 
TTTTCAGGAA TTGATGGAGC AAGAGGACCT AAAGGAAACA AAGGTGACCC TGCCAGTCAC 
TTTGGTCCAC CTGGTCCAAA GGGTGAGCCA GGTAGCCCTG GATGTCCAGG GCATTTTGGA 
GCATCTGGAG AGCAGGGCTT GCCTGGTATT CAAGGGCCCA GAGGATCACC TGGAAGGCCA 
GGGCCACCTG GCTCCTCTGG ACCACCAGGG TGCCCAGGTG ATCACGGGAT GCCTGGGCTG 
AGGGGACAGC CAGGAGAAAT GGGAGACCCT GGGCCAAGAG GCCTCCAGGG GGATCCAGGG 
ATACCAGGTC CTCCGGGAAT AAAAGGTCCC TCCGGATCAC CTGGCCTGAA CGGCTTGCAT 
GGATTGAAAG GTCAGAAAGG AACTAAAGGT GCTTCAGGTT TGCATGATGT GGGGCCACCT 
GGTCCAGTGG GAATACCTGG GCTAAAAGGG GAGAGAGGAG ACCCTGGGAG CCCAGGAATC 
TCTCCTCCAG GTCCTCGTGG AGAAAAAGGT CCCCCAGGAC CCCCAGGGAG TTCAGGACCA 
CCTGGTCCTG CAGGTGCCAC AGGAAGAGCT CCTAAGGACA TTCCTGACCC GGGTCCACCT 
GGAGATCAGG GACCTCCTGG TCCTGATGGC CCAAGAGGAG CACCTGGGCC TCCAGGCCTC 
CCTGGGAGTG TTGACCTTCT GAGAGGGGAG CCAGGTGACT GTGGTCTACC AGGGCCACCA 
GGTCCCCCTG GCCCACCAGG CCCTCCAGGA TACAAAGGCT TTCCAGGATG TGATGGAAAA 
GATGGCCAGA AAGGACCAAT GGGATTCCCG GGACCGCAGG GACCACATGG ATTTCCTGGG 
CCACCTGGAG AGAAGGGTTT ACCTGGACCT CCAGGGAGAA AAGGGCCCAC TGGTCTTCCA 
GGTCCCAGAG GTGAACCGGG GCCACCTGCA GATGTGGATG ACTGTCCCCG AATCCCAGGC 
CTTCCTGGGG CGCCAGGCAT GAGAGGACCA GAAGGAGCCA TGGGGCTCTC TGGAATGAGA 
GGCCCCCCAG GACCAGCTCA TGGGTGTGTT CTGAGGACCA AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL4A4 ( XM_009444472.3 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_009442747.3 Pan troglodytes collagen type IV alpha 4 chain (COL4A4), transcript variant X13, mRNA.

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CloneID	OPc104276
Clone ID Related Accession (Same CDS sequence)	XM_016950594.2 , XM_016950595.2 , XM_016950596.2 , XM_009444472.3
Accession Version	XM_009444472.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4245bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	collagen alpha-4(IV) chain isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036881.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_009444472.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGTCTC TGCACATAGT ACTAATGAGG TGCTCCTTCA GATTGACCAA GTCCTTGGCC 
ACAGATCCCT GGTCACTTAC ACTCATTCTC TTTTCTGTAC AATATGTATA TGGGAGTGGA 
AAGAAATACG TTGGTCCTTG TGGAGGAAGA GATTGCTCTG TTTGCCACTG TGTTCCTGAA 
AAGGGGTCTC GGGGTCCACC AGGACCACCA GGGCCACAGG GTCCAATTGG ACCCCTGGGA 
GCCCCAGGAC CCATTGGGCT TTCAGGAGAG AAAGGAATGA GAGGGGACCG CGGCCCTCCT 
GGAGCAGCAG GGGACAAAGG AGATAAGGGT TCAACTGGTG TTCCTGGATT TCCAGGTTTA 
GATGGCATAC CTGGGCACCC AGGGCCTCCT GGACCCAGAG GCAAACCTGG TATGAGTGGC 
CACAATGGCT CAAGAGGTGA CCCAGGGTTT CCAGGAGGAA GAGGAGCTCT TGGCCCAGGA 
GGCCCCCTAG GCCATCCTGG GGAAAAGGGA GAAAAAGGAA ATTCAGTGTT CATTTTAGGT 
GCCGTTAAAG GTATTCAGGG AGACAGAGGG GACCCAGGAC TGCCTGGCTT ACCAGGATCT 
TGGGGGGCAG GAGGACCGGC GGGTCCCACA GGATATCCTG GAGAGCCAGG GTTAGTGGGA 
CCTCCGGGCC AACCAGGGCG TCCAGGTTTG AAGGGAAATC CCGGTGTGGG AGTAAAGGGG 
CAAATGGGAG ACCCGGGTGA GGTTGGTCAG CAAGGTTCTC CTGGACCCAC CCTGTTGGTG 
GAGCCACCTG ACTTTCGTCT CTATAAAGGA GAAAAGGGTA TAAAAGGAAT TCCTGGAATG 
GTTGGACTGC CAGGACCACC AGGACGCAAG GGAGAATCTG ATATTGGGGC AAAAGGAGAA 
AAAGGTATTC CTGGATTTCC AGGGCCTCGG GGGGATCCTG GTTCCTATGG ATCTCCAGGT 
TTTCCAGGAT TAAAGGGAGA ACTAGGACTG GTTGGAGATC CTGGGCTATT TGGATTAATT 
GGCCCAAAGG GGGATCCTGG AAATCGAGGG CACCCAGGAC CACCAGGTGT TTTGGTGACT 
CCACCTCTTC CACTCAAAGG CCCACCAGGG GACCCAGGGT TCCCTGGCCG CTATGGAGAA 
ACAGGGGATG TTGGACCACC TGGTCCCCCA GGTCTCTTGG GCAGACCAGG GGAAGCCTGT 
GCAGGCATGA TAGGACCCCC TGGGCCACAA GGATTTCCTG GTCTTCCTGG GCTTCCAGGA 
GAAGCTGGTA TTCCTGGGAG ACCTGATTCT GCTCCAGGAA AACCAGGGAA GCCAGGATCA 
CCTGGCTTGC CTGGAGCACC AGGCCTGCAG GGCCTCCCAG GATCAAGTGT GATATACTGT 
AGTGTTGGGA ATCCTGGACC ACAAGGAATA AAAGGCAAAG TGGGTCCCCC AGGAGGAAGA 
GGCTCAAAAG GAGAAAAAGG AAATGAAGGA CTCTGTGCCT GTGAGCCTGG ACCCATGGGC 
CCCCCTGGCC CTCCAGGACT TCCTGGGAGG CAGGGGAGTA AGGGAGACTT GGGGCTCCCT 
GGCTGGCTTG GAACAAAAGG TGACCCAGGA CCTCCTGGTG CTGAAGGACC TCCAGGGCTA 
CCAGGAAAGC ATGGTGCCTC TGGACCACCT GGCAACAAAG GGGCGAAGGG TGACATGGTT 
GTATCAAGAG TTAAAGGGCA CAAAGGAGAA AGAGGTCCTG ATGGGCCCCC AGGATTTCCA 
GGGCAGCCAG GATCACATGG TCGGGATGGA CATGCTGGAG AAAAAGGGGA TCCAGGACCC 
CCAGGGGATC ATGAAGATGC GACCCCAGGT GGTAAAGGAT TTCCTGGACC TCTGGGCCCC 
CCGGGCAAAG CAGGACCTTT GGGGCCCCCA GGACTGGGAT TTCCTGGTCC ACCAGGACAG 
CGAGGCCACC CAGGAGTTCC AGGCCACCCA GGTGTGAGGG GCCCTGATGG CTTGAAGGGT 
CAGAAAGGTG ACACAATTTC TTGCAACGTA ACCTACCCTG GGAGGCACGG CCCTCCAGGT 
TTTGATGGAC CTCCAGGTCC GAAGGGATTT CCAGGTCCCC AAGGTGCCCC TGGGCTGAGT 
GGTTCAGATG GGCATAAAGG CAGACCTGGC ACACCAGGAA CAGCGGAAAT ACCAGGTCCA 
CCTGGTTTTC GTGGTGACAT GGGAGATCCG GGTTTTGGAG GTGAAAAGGG GTCCTCCCCT 
GCTGGGCCCC CAGGCCCTCC CGGCTCACCA GGAGTGAATG GTCAGAAAGG AATCCCGGGA 
GACCCTGCAT TTGGTCACCT GGGACCCCCG GGAAAGAGGG GTCTTTCAGG AGTGCCAGGG 
ATAAAAGGAC CCAGAGGTGA TCCGGGATGT CCAGGGGCTG AAGGGCCAGC TGGCATTCCT 
GGATTCCCAG GTCTCAAAGG TCCCAAAGGC AGAGAGGGAC ATGCTGGGTT TCCAGGTGTC 
CCAGGTCCAC CTGGCCATTC CTGTGAAAGA GGTGCTCCAG GGATACCAGG GCAACCGGGA 
CTCCCTGGGT ATCCAGGTAG CCCAGGTGCT CCAGGTGGGA AAGGACAGCT GGGAGATGTG 
GGGCCTCCCG GGCCAGCTGG AATGAAAGGC CTCCCTGGAC TCCCAGGATG GCCTGGGGCA 
CATGGTCCCC CAGGCCTCCC AGGAATCCCA GGTCCCTTTG GAGATGATGG GCTACCTGGT 
CCTCCAGGTC CAAAGGGACC CCAGGGGCTG CCTGGTTTCC CAGGTTTTCC CGGAGAAAGA 
GGAAAGCCTG GTGCAGAGGG ATGTCCTGGC ACAAAGGGAG AACCTGGAGA GAAGGGCATG 
TCTGGCCTTC CCGGAGACCG GGGACTGAGA GGGGCCAAAG GAGCCATAGG ACCTCCCGGA 
GATGAAGGAG AAATGGCTAT CATTTCCCAA AAGGGAACAC CTGGGGAACC TGGACCTCCT 
GGAGATGATG GATTCCCAGG AGAAAGAGGT GATAAAGGAA CTCCCGGGAT GCAAGGGAGA 
AGAGGAGAGC CGGGAAGATA CGGACCACCT GGATTTCAGA GAGGGGAACC TGGTGAGAAA 
GGTCAGCCAG GGCATCCTGG ACCCCCAGGC CCTCCAGGCT CAACTGGTCT AAGAGGGTTC 
ATTGGTTTTC CAGGACTTCC AGGTGACCAG GGTGAGCCAG GTTCTCCAGG TCCCCCTGGA 
TTTTCAGGAA TTGATGGAGC AAGAGGACCT AAAGGAAACA AAGGTGACCC TGCCAGTCAC 
TTTGGTCCAC CTGGTCCAAA GGGTGAGCCA GGTAGCCCTG GATGTCCAGG GCATTTTGGA 
GCATCTGGAG AGCAGGGCTT GCCTGGTATT CAAGGGCCCA GAGGATCACC TGGAAGGCCA 
GGGCCACCTG GCTCCTCTGG ACCACCAGGG TGCCCAGGTG ATCACGGGAT GCCTGGGCTG 
AGGGGACAGC CAGGAGAAAT GGGAGACCCT GGGCCAAGAG GCCTCCAGGG GGATCCAGGG 
ATACCAGGTC CTCCGGGAAT AAAAGGTCCC TCCGGATCAC CTGGCCTGAA CGGCTTGCAT 
GGATTGAAAG GTCAGAAAGG AACTAAAGGT GCTTCAGGTT TGCATGATGT GGGGCCACCT 
GGTCCAGTGG GAATACCTGG GCTAAAAGGG GAGAGAGGAG ACCCTGGGAG CCCAGGAATC 
TCTCCTCCAG GTCCTCGTGG AGAAAAAGGT CCCCCAGGAC CCCCAGGGAG TTCAGGACCA 
CCTGGTCCTG CAGGTGCCAC AGGAAGAGCT CCTAAGGACA TTCCTGACCC GGGTCCACCT 
GGAGATCAGG GACCTCCTGG TCCTGATGGC CCAAGAGGAG CACCTGGGCC TCCAGGCCTC 
CCTGGGAGTG TTGACCTTCT GAGAGGGGAG CCAGGTGACT GTGGTCTACC AGGGCCACCA 
GGTCCCCCTG GCCCACCAGG CCCTCCAGGA TACAAAGGCT TTCCAGGATG TGATGGAAAA 
GATGGCCAGA AAGGACCAAT GGGATTCCCG GGACCGCAGG GACCACATGG ATTTCCTGGG 
CCACCTGGAG AGAAGGGTTT ACCTGGACCT CCAGGGAGAA AAGGGCCCAC TGGTCTTCCA 
GGTCCCAGAG GTGAACCGGG GCCACCTGCA GATGTGGATG ACTGTCCCCG AATCCCAGGC 
CTTCCTGGGG CGCCAGGCAT GAGAGGACCA GAAGGAGCCA TGGGGCTCTC TGGAATGAGA 
GGCCCCCCAG GACCAGCTCA TGGGTGTGTT CTGAGGACCA AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009442747.3
CDS	422..4666
Translation	MWSLHIVLMRCSFRLTKSLATDPWSLTLILFSVQYVYGSGKKYVGPCGGRDCSVCHCVPEKGSRGPPGPPGPQGPIGPLGAPGPIGLSGEKGMRGDRGPPGAAGDKGDKGSTGVPGFPGLDGIPGHPGPPGPRGKPGMSGHNGSRGDPGFPGGRGALGPGGPLGHPGEKGEKGNSVFILGAVKGIQGDRGDPGLPGLPGSWGAGGPAGPTGYPGEPGLVGPPGQPGRPGLKGNPGVGVKGQMGDPGEVGQQGSPGPTLLVEPPDFRLYKGEKGIKGIPGMVGLPGPPGRKGESDIGAKGEKGIPGFPGPRGDPGSYGSPGFPGLKGELGLVGDPGLFGLIGPKGDPGNRGHPGPPGVLVTPPLPLKGPPGDPGFPGRYGETGDVGPPGPPGLLGRPGEACAGMIGPPGPQGFPGLPGLPGEAGIPGRPDSAPGKPGKPGSPGLPGAPGLQGLPGSSVIYCSVGNPGPQGIKGKVGPPGGRGSKGEKGNEGLCACEPGPMGPPGPPGLPGRQGSKGDLGLPGWLGTKGDPGPPGAEGPPGLPGKHGASGPPGNKGAKGDMVVSRVKGHKGERGPDGPPGFPGQPGSHGRDGHAGEKGDPGPPGDHEDATPGGKGFPGPLGPPGKAGPLGPPGLGFPGPPGQRGHPGVPGHPGVRGPDGLKGQKGDTISCNVTYPGRHGPPGFDGPPGPKGFPGPQGAPGLSGSDGHKGRPGTPGTAEIPGPPGFRGDMGDPGFGGEKGSSPAGPPGPPGSPGVNGQKGIPGDPAFGHLGPPGKRGLSGVPGIKGPRGDPGCPGAEGPAGIPGFPGLKGPKGREGHAGFPGVPGPPGHSCERGAPGIPGQPGLPGYPGSPGAPGGKGQLGDVGPPGPAGMKGLPGLPGWPGAHGPPGLPGIPGPFGDDGLPGPPGPKGPQGLPGFPGFPGERGKPGAEGCPGTKGEPGEKGMSGLPGDRGLRGAKGAIGPPGDEGEMAIISQKGTPGEPGPPGDDGFPGERGDKGTPGMQGRRGEPGRYGPPGFQRGEPGEKGQPGHPGPPGPPGSTGLRGFIGFPGLPGDQGEPGSPGPPGFSGIDGARGPKGNKGDPASHFGPPGPKGEPGSPGCPGHFGASGEQGLPGIQGPRGSPGRPGPPGSSGPPGCPGDHGMPGLRGQPGEMGDPGPRGLQGDPGIPGPPGIKGPSGSPGLNGLHGLKGQKGTKGASGLHDVGPPGPVGIPGLKGERGDPGSPGISPPGPRGEKGPPGPPGSSGPPGPAGATGRAPKDIPDPGPPGDQGPPGPDGPRGAPGPPGLPGSVDLLRGEPGDCGLPGPPGPPGPPGPPGYKGFPGCDGKDGQKGPMGFPGPQGPHGFPGPPGEKGLPGPPGRKGPTGLPGPRGEPGPPADVDDCPRIPGLPGAPGMRGPEGAMGLSGMRGPPGPAHGCVLRTK

Target ORF information:

RefSeq Version	XM_009444472.3
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes collagen type IV alpha 4 chain (COL4A4), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009444472.3

ORF Insert Sequence:

ATGTGGTCTC TGCACATAGT ACTAATGAGG TGCTCCTTCA GATTGACCAA GTCCTTGGCC 
ACAGATCCCT GGTCACTTAC ACTCATTCTC TTTTCTGTAC AATATGTATA TGGGAGTGGA 
AAGAAATACG TTGGTCCTTG TGGAGGAAGA GATTGCTCTG TTTGCCACTG TGTTCCTGAA 
AAGGGGTCTC GGGGTCCACC AGGACCACCA GGGCCACAGG GTCCAATTGG ACCCCTGGGA 
GCCCCAGGAC CCATTGGGCT TTCAGGAGAG AAAGGAATGA GAGGGGACCG CGGCCCTCCT 
GGAGCAGCAG GGGACAAAGG AGATAAGGGT TCAACTGGTG TTCCTGGATT TCCAGGTTTA 
GATGGCATAC CTGGGCACCC AGGGCCTCCT GGACCCAGAG GCAAACCTGG TATGAGTGGC 
CACAATGGCT CAAGAGGTGA CCCAGGGTTT CCAGGAGGAA GAGGAGCTCT TGGCCCAGGA 
GGCCCCCTAG GCCATCCTGG GGAAAAGGGA GAAAAAGGAA ATTCAGTGTT CATTTTAGGT 
GCCGTTAAAG GTATTCAGGG AGACAGAGGG GACCCAGGAC TGCCTGGCTT ACCAGGATCT 
TGGGGGGCAG GAGGACCGGC GGGTCCCACA GGATATCCTG GAGAGCCAGG GTTAGTGGGA 
CCTCCGGGCC AACCAGGGCG TCCAGGTTTG AAGGGAAATC CCGGTGTGGG AGTAAAGGGG 
CAAATGGGAG ACCCGGGTGA GGTTGGTCAG CAAGGTTCTC CTGGACCCAC CCTGTTGGTG 
GAGCCACCTG ACTTTCGTCT CTATAAAGGA GAAAAGGGTA TAAAAGGAAT TCCTGGAATG 
GTTGGACTGC CAGGACCACC AGGACGCAAG GGAGAATCTG ATATTGGGGC AAAAGGAGAA 
AAAGGTATTC CTGGATTTCC AGGGCCTCGG GGGGATCCTG GTTCCTATGG ATCTCCAGGT 
TTTCCAGGAT TAAAGGGAGA ACTAGGACTG GTTGGAGATC CTGGGCTATT TGGATTAATT 
GGCCCAAAGG GGGATCCTGG AAATCGAGGG CACCCAGGAC CACCAGGTGT TTTGGTGACT 
CCACCTCTTC CACTCAAAGG CCCACCAGGG GACCCAGGGT TCCCTGGCCG CTATGGAGAA 
ACAGGGGATG TTGGACCACC TGGTCCCCCA GGTCTCTTGG GCAGACCAGG GGAAGCCTGT 
GCAGGCATGA TAGGACCCCC TGGGCCACAA GGATTTCCTG GTCTTCCTGG GCTTCCAGGA 
GAAGCTGGTA TTCCTGGGAG ACCTGATTCT GCTCCAGGAA AACCAGGGAA GCCAGGATCA 
CCTGGCTTGC CTGGAGCACC AGGCCTGCAG GGCCTCCCAG GATCAAGTGT GATATACTGT 
AGTGTTGGGA ATCCTGGACC ACAAGGAATA AAAGGCAAAG TGGGTCCCCC AGGAGGAAGA 
GGCTCAAAAG GAGAAAAAGG AAATGAAGGA CTCTGTGCCT GTGAGCCTGG ACCCATGGGC 
CCCCCTGGCC CTCCAGGACT TCCTGGGAGG CAGGGGAGTA AGGGAGACTT GGGGCTCCCT 
GGCTGGCTTG GAACAAAAGG TGACCCAGGA CCTCCTGGTG CTGAAGGACC TCCAGGGCTA 
CCAGGAAAGC ATGGTGCCTC TGGACCACCT GGCAACAAAG GGGCGAAGGG TGACATGGTT 
GTATCAAGAG TTAAAGGGCA CAAAGGAGAA AGAGGTCCTG ATGGGCCCCC AGGATTTCCA 
GGGCAGCCAG GATCACATGG TCGGGATGGA CATGCTGGAG AAAAAGGGGA TCCAGGACCC 
CCAGGGGATC ATGAAGATGC GACCCCAGGT GGTAAAGGAT TTCCTGGACC TCTGGGCCCC 
CCGGGCAAAG CAGGACCTTT GGGGCCCCCA GGACTGGGAT TTCCTGGTCC ACCAGGACAG 
CGAGGCCACC CAGGAGTTCC AGGCCACCCA GGTGTGAGGG GCCCTGATGG CTTGAAGGGT 
CAGAAAGGTG ACACAATTTC TTGCAACGTA ACCTACCCTG GGAGGCACGG CCCTCCAGGT 
TTTGATGGAC CTCCAGGTCC GAAGGGATTT CCAGGTCCCC AAGGTGCCCC TGGGCTGAGT 
GGTTCAGATG GGCATAAAGG CAGACCTGGC ACACCAGGAA CAGCGGAAAT ACCAGGTCCA 
CCTGGTTTTC GTGGTGACAT GGGAGATCCG GGTTTTGGAG GTGAAAAGGG GTCCTCCCCT 
GCTGGGCCCC CAGGCCCTCC CGGCTCACCA GGAGTGAATG GTCAGAAAGG AATCCCGGGA 
GACCCTGCAT TTGGTCACCT GGGACCCCCG GGAAAGAGGG GTCTTTCAGG AGTGCCAGGG 
ATAAAAGGAC CCAGAGGTGA TCCGGGATGT CCAGGGGCTG AAGGGCCAGC TGGCATTCCT 
GGATTCCCAG GTCTCAAAGG TCCCAAAGGC AGAGAGGGAC ATGCTGGGTT TCCAGGTGTC 
CCAGGTCCAC CTGGCCATTC CTGTGAAAGA GGTGCTCCAG GGATACCAGG GCAACCGGGA 
CTCCCTGGGT ATCCAGGTAG CCCAGGTGCT CCAGGTGGGA AAGGACAGCT GGGAGATGTG 
GGGCCTCCCG GGCCAGCTGG AATGAAAGGC CTCCCTGGAC TCCCAGGATG GCCTGGGGCA 
CATGGTCCCC CAGGCCTCCC AGGAATCCCA GGTCCCTTTG GAGATGATGG GCTACCTGGT 
CCTCCAGGTC CAAAGGGACC CCAGGGGCTG CCTGGTTTCC CAGGTTTTCC CGGAGAAAGA 
GGAAAGCCTG GTGCAGAGGG ATGTCCTGGC ACAAAGGGAG AACCTGGAGA GAAGGGCATG 
TCTGGCCTTC CCGGAGACCG GGGACTGAGA GGGGCCAAAG GAGCCATAGG ACCTCCCGGA 
GATGAAGGAG AAATGGCTAT CATTTCCCAA AAGGGAACAC CTGGGGAACC TGGACCTCCT 
GGAGATGATG GATTCCCAGG AGAAAGAGGT GATAAAGGAA CTCCCGGGAT GCAAGGGAGA 
AGAGGAGAGC CGGGAAGATA CGGACCACCT GGATTTCAGA GAGGGGAACC TGGTGAGAAA 
GGTCAGCCAG GGCATCCTGG ACCCCCAGGC CCTCCAGGCT CAACTGGTCT AAGAGGGTTC 
ATTGGTTTTC CAGGACTTCC AGGTGACCAG GGTGAGCCAG GTTCTCCAGG TCCCCCTGGA 
TTTTCAGGAA TTGATGGAGC AAGAGGACCT AAAGGAAACA AAGGTGACCC TGCCAGTCAC 
TTTGGTCCAC CTGGTCCAAA GGGTGAGCCA GGTAGCCCTG GATGTCCAGG GCATTTTGGA 
GCATCTGGAG AGCAGGGCTT GCCTGGTATT CAAGGGCCCA GAGGATCACC TGGAAGGCCA 
GGGCCACCTG GCTCCTCTGG ACCACCAGGG TGCCCAGGTG ATCACGGGAT GCCTGGGCTG 
AGGGGACAGC CAGGAGAAAT GGGAGACCCT GGGCCAAGAG GCCTCCAGGG GGATCCAGGG 
ATACCAGGTC CTCCGGGAAT AAAAGGTCCC TCCGGATCAC CTGGCCTGAA CGGCTTGCAT 
GGATTGAAAG GTCAGAAAGG AACTAAAGGT GCTTCAGGTT TGCATGATGT GGGGCCACCT 
GGTCCAGTGG GAATACCTGG GCTAAAAGGG GAGAGAGGAG ACCCTGGGAG CCCAGGAATC 
TCTCCTCCAG GTCCTCGTGG AGAAAAAGGT CCCCCAGGAC CCCCAGGGAG TTCAGGACCA 
CCTGGTCCTG CAGGTGCCAC AGGAAGAGCT CCTAAGGACA TTCCTGACCC GGGTCCACCT 
GGAGATCAGG GACCTCCTGG TCCTGATGGC CCAAGAGGAG CACCTGGGCC TCCAGGCCTC 
CCTGGGAGTG TTGACCTTCT GAGAGGGGAG CCAGGTGACT GTGGTCTACC AGGGCCACCA 
GGTCCCCCTG GCCCACCAGG CCCTCCAGGA TACAAAGGCT TTCCAGGATG TGATGGAAAA 
GATGGCCAGA AAGGACCAAT GGGATTCCCG GGACCGCAGG GACCACATGG ATTTCCTGGG 
CCACCTGGAG AGAAGGGTTT ACCTGGACCT CCAGGGAGAA AAGGGCCCAC TGGTCTTCCA 
GGTCCCAGAG GTGAACCGGG GCCACCTGCA GATGTGGATG ACTGTCCCCG AATCCCAGGC 
CTTCCTGGGG CGCCAGGCAT GAGAGGACCA GAAGGAGCCA TGGGGCTCTC TGGAATGAGA 
GGCCCCCCAG GACCAGCTCA TGGGTGTGTT CTGAGGACCA AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL4A4 ( XM_024355097.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_024210865.1 Pan troglodytes collagen type IV alpha 4 chain (COL4A4), transcript variant X17, mRNA.

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CloneID	OPc104277
Clone ID Related Accession (Same CDS sequence)	XM_024355097.1
Accession Version	XM_024355097.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4026bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	collagen alpha-4(IV) chain isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036881.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGTCTC TGCACATAGT ACTAATGAGG TGCTCCTTCA GATTGACCAA GTCCTTGGCC 
ACAGATCCCT GGTCACTTAC ACTCATTCTC TTTTCTGTAC AATATGTATA TGGGAGTGGA 
AAGAAATACG TTGGTCCTTG TGGAGGAAGA GATTGCTCTG TTTGCCACTG TGTTCCTGAA 
AAGGGGTCTC GGGGTCCACC AGGACCACCA GGGCCACAGG GTCCAATTGG ACCCCTGGGA 
GCCCCAGGAC CCATTGGGCT TTCAGGAGAG AAAGGAATGA GAGGGGACCG CGGCCCTCCT 
GGAGCAGCAG GGGACAAAGG AGATAAGGGT TCAACTGGTG TTCCTGGATT TCCAGGTTTA 
GATGGCATAC CTGGGCACCC AGGGCCTCCT GGACCCAGAG GCAAACCTGG TATGAGTGGC 
CACAATGGCT CAAGAGGTGA CCCAGGGTTT CCAGGAGGAA GAGGAGCTCT TGGCCCAGGA 
GGCCCCCTAG GCCATCCTGG GGAAAAGGGA GAAAAAGGAA ATTCAGTGTT CATTTTAGGT 
GCCGTTAAAG GTATTCAGGG AGACAGAGGG GACCCAGGAC TGCCTGGCTT ACCAGGATCT 
TGGGGGGCAG GAGGACCGGC GGGTCCCACA GGATATCCTG GAGAGCCAGG GTTAGTGGGA 
CCTCCGGGCC AACCAGGGCG TCCAGGTTTG AAGGGAAATC CCGGTGTGGG AGTAAAGGGG 
CAAATGGGAG ACCCGGGTGA GGTTGGTCAG CAAGGTTCTC CTGGACCCAC CCTGTTGGTG 
GAGCCACCTG ACTTTCGTCT CTATAAAGGA GAAAAGGGTA TAAAAGGAAT TCCTGGAATG 
GTTGGACTGC CAGGACCACC AGGACGCAAG GGAGAATCTG ATATTGGGGC AAAAGGAGAA 
AAAGGTATTC CTGGATTTCC AGGGCCTCGG GGGGATCCTG GTTCCTATGG ATCTCCAGGT 
TTTCCAGGAT TAAAGGGAGA ACTAGGACTG GTTGGAGATC CTGGGCTATT TGGATTAATT 
GGCCCAAAGG GGGATCCTGG AAATCGAGGG CACCCAGGAC CACCAGGTGT TTTGGTGACT 
CCACCTCTTC CACTCAAAGG CCCACCAGGG GACCCAGGGT TCCCTGGCCG CTATGGAGAA 
ACAGGGGATG TTGGACCACC TGGTCCCCCA GGTCTCTTGG GCAGACCAGG GGAAGCCTGT 
GCAGGCATGA TAGGACCCCC TGGGCCACAA GGATTTCCTG GTCTTCCTGG GCTTCCAGGA 
GAAGCTGGTA TTCCTGGGAG ACCTGATTCT GCTCCAGGAA AACCAGGGAA GCCAGGATCA 
CCTGGCTTGC CTGGAGCACC AGGCCTGCAG GGCCTCCCAG GATCAAGTGT GATATACTGT 
AGTGTTGGGA ATCCTGGACC ACAAGGAATA AAAGGCAAAG TGGGTCCCCC AGGAGGAAGA 
GGCTCAAAAG GAGAAAAAGG AAATGAAGGA CTCTGTGCCT GTGAGCCTGG ACCCATGGGC 
CCCCCTGGCC CTCCAGGACT TCCTGGGAGG CAGGGGAGTA AGGGAGACTT GGGGCTCCCT 
GGCTGGCTTG GAACAAAAGG TGACCCAGGA CCTCCTGGTG CTGAAGGACC TCCAGGGCTA 
CCAGGAAAGC ATGGTGCCTC TGGACCACCT GGCAACAAAG GGGCGAAGGG TGACATGGTT 
GTATCAAGAG TTAAAGGGCA CAAAGGAGAA AGAGGTCCTG ATGGGCCCCC AGGATTTCCA 
GGGCAGCCAG GATCACATGG TCGGGATGGA CATGCTGGAG AAAAAGGGGA TCCAGGACCC 
CCAGGGGATC ATGAAGATGC GACCCCAGGT GGTAAAGGAT TTCCTGGACC TCTGGGCCCC 
CCGGGCAAAG CAGGACCTTT GGGGCCCCCA GGACTGGGAT TTCCTGGTCC ACCAGGACAG 
CGAGGCCACC CAGGAGTTCC AGGCCACCCA GGTGTGAGGG GCCCTGATGG CTTGAAGGGT 
CAGAAAGGTG ACACAATTTC TTGCAACGTA ACCTACCCTG GGAGGCACGG CCCTCCAGGT 
TTTGATGGAC CTCCAGGTCC GAAGGGATTT CCAGGTCCCC AAGGTGCCCC TGGGCTGAGT 
GGTTCAGATG GGCATAAAGG CAGACCTGGC ACACCAGGAA CAGCGGAAAT ACCAGGTCCA 
CCTGGTTTTC GTGGTGACAT GGGAGATCCG GGTTTTGGAG GTGAAAAGGG GTCCTCCCCT 
GCTGGGCCCC CAGGCCCTCC CGGCTCACCA GGAGTGAATG GTCAGAAAGG AATCCCGGGA 
GACCCTGCAT TTGGTCACCT GGGACCCCCG GGAAAGAGGG GTCTTTCAGG AGTGCCAGGG 
ATAAAAGGAC CCAGAGGTGA TCCGGGATGT CCAGGGGCTG AAGGGCCAGC TGGCATTCCT 
GGATTCCCAG GTCTCAAAGG TCCCAAAGGC AGAGAGGGAC ATGCTGGGTT TCCAGGTGTC 
CCAGGTCCAC CTGGCCATTC CTGTGAAAGA GGTGCTCCAG GGATACCAGG GCAACCGGGA 
CTCCCTGGGT ATCCAGGTAG CCCAGGTGCT CCAGGTGGGA AAGGACAGCT GGGAGATGTG 
GGGCCTCCCG GGCCAGCTGG AATGAAAGGC CTCCCTGGAC TCCCAGGATG GCCTGGGGCA 
CATGGTCCCC CAGGCCTCCC AGGAATCCCA GGTCCCTTTG GAGATGATGG GCTACCTGGT 
CCTCCAGGTC CAAAGGGACC CCAGGGGCTG CCTGGTTTCC CAGGTTTTCC CGGAGAAAGA 
GGAAAGCCTG GTGCAGAGGG ATGTCCTGGC ACAAAGGGAG AACCTGGAGA GAAGGGCATG 
TCTGGCCTTC CCGGAGACCG GGGACTGAGA GGGGCCAAAG GAGCCATAGG ACCTCCCGGA 
GATGAAGGAG AAATGGCTAT CATTTCCCAA AAGGGAACAC CTGGGGAACC TGGACCTCCT 
GGAGATGATG GATTCCCAGG AGAAAGAGGT GATAAAGGAA CTCCCGGGAT GCAAGGGAGA 
AGAGGAGAGC CGGGAAGATA CGGACCACCT GGATTTCAGA GAGGGGAACC TGGTGAGAAA 
GGTCAGCCAG GGCATCCTGG ACCCCCAGGC CCTCCAGGCT CAACTGGTCT AAGAGGGTTC 
ATTGGTTTTC CAGGACTTCC AGGTGACCAG GGTGAGCCAG GTTCTCCAGG TCCCCCTGGA 
TTTTCAGGAA TTGATGGAGC AAGAGGACCT AAAGGAAACA AAGGTGACCC TGCCAGTCAC 
TTTGGTCCAC CTGGTCCAAA GGGTGAGCCA GGTAGCCCTG GATGTCCAGG GCATTTTGGA 
GCATCTGGAG AGCAGGGCTT GCCTGGTATT CAAGGGCCCA GAGGATCACC TGGAAGGCCA 
GGGCCACCTG GCTCCTCTGG ACCACCAGGG TGCCCAGGTG ATCACGGGAT GCCTGGGCTG 
AGGGGACAGC CAGGAGAAAT GGGAGACCCT GGGCCAAGAG GCCTCCAGGG GGATCCAGGG 
ATACCAGGTC CTCCGGGAAT AAAAGGTCCC TCCGGATCAC CTGGCCTGAA CGGCTTGCAT 
GGATTGAAAG GTCAGAAAGG AACTAAAGGT GCTTCAGGTT TGCATGATGT GGGGCCACCT 
GGTCCAGTGG GAATACCTGG GCTAAAAGGG GAGAGAGGAG ACCCTGGGAG CCCAGGAATC 
TCTCCTCCAG GTCCTCGTGG AGAAAAAGGT CCCCCAGGAC CCCCAGGGAG TTCAGGACCA 
CCTGGTCCTG CAGGTGCCAC AGGAAGAGCT CCTAAGGACA TTCCTGACCC GGGTCCACCT 
GGAGATCAGG GACCTCCTGG TCCTGATGGC CCAAGAGGAG CACCTGGGCC TCCAGGCCTC 
CCTGGGAGTG TTGACCTTCT GAGAGGGGAG CCAGGTGACT GTGGTCTACC AGGGCCACCA 
GGTCCCCCTG GCCCACCAGG CCCTCCAGGA TACAAAGGCT TTCCAGGATG TGATGGAAAA 
GATGGCCAGA AAGGAAGCTG GATATCAGAA AAGCAACAGT TGAAATTTGC AAAAGGAGCC 
CAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024210865.1
CDS	423..4448
Translation	MWSLHIVLMRCSFRLTKSLATDPWSLTLILFSVQYVYGSGKKYVGPCGGRDCSVCHCVPEKGSRGPPGPPGPQGPIGPLGAPGPIGLSGEKGMRGDRGPPGAAGDKGDKGSTGVPGFPGLDGIPGHPGPPGPRGKPGMSGHNGSRGDPGFPGGRGALGPGGPLGHPGEKGEKGNSVFILGAVKGIQGDRGDPGLPGLPGSWGAGGPAGPTGYPGEPGLVGPPGQPGRPGLKGNPGVGVKGQMGDPGEVGQQGSPGPTLLVEPPDFRLYKGEKGIKGIPGMVGLPGPPGRKGESDIGAKGEKGIPGFPGPRGDPGSYGSPGFPGLKGELGLVGDPGLFGLIGPKGDPGNRGHPGPPGVLVTPPLPLKGPPGDPGFPGRYGETGDVGPPGPPGLLGRPGEACAGMIGPPGPQGFPGLPGLPGEAGIPGRPDSAPGKPGKPGSPGLPGAPGLQGLPGSSVIYCSVGNPGPQGIKGKVGPPGGRGSKGEKGNEGLCACEPGPMGPPGPPGLPGRQGSKGDLGLPGWLGTKGDPGPPGAEGPPGLPGKHGASGPPGNKGAKGDMVVSRVKGHKGERGPDGPPGFPGQPGSHGRDGHAGEKGDPGPPGDHEDATPGGKGFPGPLGPPGKAGPLGPPGLGFPGPPGQRGHPGVPGHPGVRGPDGLKGQKGDTISCNVTYPGRHGPPGFDGPPGPKGFPGPQGAPGLSGSDGHKGRPGTPGTAEIPGPPGFRGDMGDPGFGGEKGSSPAGPPGPPGSPGVNGQKGIPGDPAFGHLGPPGKRGLSGVPGIKGPRGDPGCPGAEGPAGIPGFPGLKGPKGREGHAGFPGVPGPPGHSCERGAPGIPGQPGLPGYPGSPGAPGGKGQLGDVGPPGPAGMKGLPGLPGWPGAHGPPGLPGIPGPFGDDGLPGPPGPKGPQGLPGFPGFPGERGKPGAEGCPGTKGEPGEKGMSGLPGDRGLRGAKGAIGPPGDEGEMAIISQKGTPGEPGPPGDDGFPGERGDKGTPGMQGRRGEPGRYGPPGFQRGEPGEKGQPGHPGPPGPPGSTGLRGFIGFPGLPGDQGEPGSPGPPGFSGIDGARGPKGNKGDPASHFGPPGPKGEPGSPGCPGHFGASGEQGLPGIQGPRGSPGRPGPPGSSGPPGCPGDHGMPGLRGQPGEMGDPGPRGLQGDPGIPGPPGIKGPSGSPGLNGLHGLKGQKGTKGASGLHDVGPPGPVGIPGLKGERGDPGSPGISPPGPRGEKGPPGPPGSSGPPGPAGATGRAPKDIPDPGPPGDQGPPGPDGPRGAPGPPGLPGSVDLLRGEPGDCGLPGPPGPPGPPGPPGYKGFPGCDGKDGQKGSWISEKQQLKFAKGAQ

Target ORF information:

RefSeq Version	XM_024355097.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes collagen type IV alpha 4 chain (COL4A4), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024355097.1

ORF Insert Sequence:

ATGTGGTCTC TGCACATAGT ACTAATGAGG TGCTCCTTCA GATTGACCAA GTCCTTGGCC 
ACAGATCCCT GGTCACTTAC ACTCATTCTC TTTTCTGTAC AATATGTATA TGGGAGTGGA 
AAGAAATACG TTGGTCCTTG TGGAGGAAGA GATTGCTCTG TTTGCCACTG TGTTCCTGAA 
AAGGGGTCTC GGGGTCCACC AGGACCACCA GGGCCACAGG GTCCAATTGG ACCCCTGGGA 
GCCCCAGGAC CCATTGGGCT TTCAGGAGAG AAAGGAATGA GAGGGGACCG CGGCCCTCCT 
GGAGCAGCAG GGGACAAAGG AGATAAGGGT TCAACTGGTG TTCCTGGATT TCCAGGTTTA 
GATGGCATAC CTGGGCACCC AGGGCCTCCT GGACCCAGAG GCAAACCTGG TATGAGTGGC 
CACAATGGCT CAAGAGGTGA CCCAGGGTTT CCAGGAGGAA GAGGAGCTCT TGGCCCAGGA 
GGCCCCCTAG GCCATCCTGG GGAAAAGGGA GAAAAAGGAA ATTCAGTGTT CATTTTAGGT 
GCCGTTAAAG GTATTCAGGG AGACAGAGGG GACCCAGGAC TGCCTGGCTT ACCAGGATCT 
TGGGGGGCAG GAGGACCGGC GGGTCCCACA GGATATCCTG GAGAGCCAGG GTTAGTGGGA 
CCTCCGGGCC AACCAGGGCG TCCAGGTTTG AAGGGAAATC CCGGTGTGGG AGTAAAGGGG 
CAAATGGGAG ACCCGGGTGA GGTTGGTCAG CAAGGTTCTC CTGGACCCAC CCTGTTGGTG 
GAGCCACCTG ACTTTCGTCT CTATAAAGGA GAAAAGGGTA TAAAAGGAAT TCCTGGAATG 
GTTGGACTGC CAGGACCACC AGGACGCAAG GGAGAATCTG ATATTGGGGC AAAAGGAGAA 
AAAGGTATTC CTGGATTTCC AGGGCCTCGG GGGGATCCTG GTTCCTATGG ATCTCCAGGT 
TTTCCAGGAT TAAAGGGAGA ACTAGGACTG GTTGGAGATC CTGGGCTATT TGGATTAATT 
GGCCCAAAGG GGGATCCTGG AAATCGAGGG CACCCAGGAC CACCAGGTGT TTTGGTGACT 
CCACCTCTTC CACTCAAAGG CCCACCAGGG GACCCAGGGT TCCCTGGCCG CTATGGAGAA 
ACAGGGGATG TTGGACCACC TGGTCCCCCA GGTCTCTTGG GCAGACCAGG GGAAGCCTGT 
GCAGGCATGA TAGGACCCCC TGGGCCACAA GGATTTCCTG GTCTTCCTGG GCTTCCAGGA 
GAAGCTGGTA TTCCTGGGAG ACCTGATTCT GCTCCAGGAA AACCAGGGAA GCCAGGATCA 
CCTGGCTTGC CTGGAGCACC AGGCCTGCAG GGCCTCCCAG GATCAAGTGT GATATACTGT 
AGTGTTGGGA ATCCTGGACC ACAAGGAATA AAAGGCAAAG TGGGTCCCCC AGGAGGAAGA 
GGCTCAAAAG GAGAAAAAGG AAATGAAGGA CTCTGTGCCT GTGAGCCTGG ACCCATGGGC 
CCCCCTGGCC CTCCAGGACT TCCTGGGAGG CAGGGGAGTA AGGGAGACTT GGGGCTCCCT 
GGCTGGCTTG GAACAAAAGG TGACCCAGGA CCTCCTGGTG CTGAAGGACC TCCAGGGCTA 
CCAGGAAAGC ATGGTGCCTC TGGACCACCT GGCAACAAAG GGGCGAAGGG TGACATGGTT 
GTATCAAGAG TTAAAGGGCA CAAAGGAGAA AGAGGTCCTG ATGGGCCCCC AGGATTTCCA 
GGGCAGCCAG GATCACATGG TCGGGATGGA CATGCTGGAG AAAAAGGGGA TCCAGGACCC 
CCAGGGGATC ATGAAGATGC GACCCCAGGT GGTAAAGGAT TTCCTGGACC TCTGGGCCCC 
CCGGGCAAAG CAGGACCTTT GGGGCCCCCA GGACTGGGAT TTCCTGGTCC ACCAGGACAG 
CGAGGCCACC CAGGAGTTCC AGGCCACCCA GGTGTGAGGG GCCCTGATGG CTTGAAGGGT 
CAGAAAGGTG ACACAATTTC TTGCAACGTA ACCTACCCTG GGAGGCACGG CCCTCCAGGT 
TTTGATGGAC CTCCAGGTCC GAAGGGATTT CCAGGTCCCC AAGGTGCCCC TGGGCTGAGT 
GGTTCAGATG GGCATAAAGG CAGACCTGGC ACACCAGGAA CAGCGGAAAT ACCAGGTCCA 
CCTGGTTTTC GTGGTGACAT GGGAGATCCG GGTTTTGGAG GTGAAAAGGG GTCCTCCCCT 
GCTGGGCCCC CAGGCCCTCC CGGCTCACCA GGAGTGAATG GTCAGAAAGG AATCCCGGGA 
GACCCTGCAT TTGGTCACCT GGGACCCCCG GGAAAGAGGG GTCTTTCAGG AGTGCCAGGG 
ATAAAAGGAC CCAGAGGTGA TCCGGGATGT CCAGGGGCTG AAGGGCCAGC TGGCATTCCT 
GGATTCCCAG GTCTCAAAGG TCCCAAAGGC AGAGAGGGAC ATGCTGGGTT TCCAGGTGTC 
CCAGGTCCAC CTGGCCATTC CTGTGAAAGA GGTGCTCCAG GGATACCAGG GCAACCGGGA 
CTCCCTGGGT ATCCAGGTAG CCCAGGTGCT CCAGGTGGGA AAGGACAGCT GGGAGATGTG 
GGGCCTCCCG GGCCAGCTGG AATGAAAGGC CTCCCTGGAC TCCCAGGATG GCCTGGGGCA 
CATGGTCCCC CAGGCCTCCC AGGAATCCCA GGTCCCTTTG GAGATGATGG GCTACCTGGT 
CCTCCAGGTC CAAAGGGACC CCAGGGGCTG CCTGGTTTCC CAGGTTTTCC CGGAGAAAGA 
GGAAAGCCTG GTGCAGAGGG ATGTCCTGGC ACAAAGGGAG AACCTGGAGA GAAGGGCATG 
TCTGGCCTTC CCGGAGACCG GGGACTGAGA GGGGCCAAAG GAGCCATAGG ACCTCCCGGA 
GATGAAGGAG AAATGGCTAT CATTTCCCAA AAGGGAACAC CTGGGGAACC TGGACCTCCT 
GGAGATGATG GATTCCCAGG AGAAAGAGGT GATAAAGGAA CTCCCGGGAT GCAAGGGAGA 
AGAGGAGAGC CGGGAAGATA CGGACCACCT GGATTTCAGA GAGGGGAACC TGGTGAGAAA 
GGTCAGCCAG GGCATCCTGG ACCCCCAGGC CCTCCAGGCT CAACTGGTCT AAGAGGGTTC 
ATTGGTTTTC CAGGACTTCC AGGTGACCAG GGTGAGCCAG GTTCTCCAGG TCCCCCTGGA 
TTTTCAGGAA TTGATGGAGC AAGAGGACCT AAAGGAAACA AAGGTGACCC TGCCAGTCAC 
TTTGGTCCAC CTGGTCCAAA GGGTGAGCCA GGTAGCCCTG GATGTCCAGG GCATTTTGGA 
GCATCTGGAG AGCAGGGCTT GCCTGGTATT CAAGGGCCCA GAGGATCACC TGGAAGGCCA 
GGGCCACCTG GCTCCTCTGG ACCACCAGGG TGCCCAGGTG ATCACGGGAT GCCTGGGCTG 
AGGGGACAGC CAGGAGAAAT GGGAGACCCT GGGCCAAGAG GCCTCCAGGG GGATCCAGGG 
ATACCAGGTC CTCCGGGAAT AAAAGGTCCC TCCGGATCAC CTGGCCTGAA CGGCTTGCAT 
GGATTGAAAG GTCAGAAAGG AACTAAAGGT GCTTCAGGTT TGCATGATGT GGGGCCACCT 
GGTCCAGTGG GAATACCTGG GCTAAAAGGG GAGAGAGGAG ACCCTGGGAG CCCAGGAATC 
TCTCCTCCAG GTCCTCGTGG AGAAAAAGGT CCCCCAGGAC CCCCAGGGAG TTCAGGACCA 
CCTGGTCCTG CAGGTGCCAC AGGAAGAGCT CCTAAGGACA TTCCTGACCC GGGTCCACCT 
GGAGATCAGG GACCTCCTGG TCCTGATGGC CCAAGAGGAG CACCTGGGCC TCCAGGCCTC 
CCTGGGAGTG TTGACCTTCT GAGAGGGGAG CCAGGTGACT GTGGTCTACC AGGGCCACCA 
GGTCCCCCTG GCCCACCAGG CCCTCCAGGA TACAAAGGCT TTCCAGGATG TGATGGAAAA 
GATGGCCAGA AAGGAAGCTG GATATCAGAA AAGCAACAGT TGAAATTTGC AAAAGGAGCC 
CAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL4A4 ( XM_009444465.3 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_009442740.3 Pan troglodytes collagen type IV alpha 4 chain (COL4A4), transcript variant X4, mRNA.

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CloneID	OPc104269
Clone ID Related Accession (Same CDS sequence)	XM_009444465.3 , XM_516128.5 , XM_009444464.3 , XM_016950591.2
Accession Version	XM_009444465.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5073bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	collagen alpha-4(IV) chain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036881.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_009444465.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGTCTC TGCACATAGT ACTAATGAGG TGCTCCTTCA GATTGACCAA GTCCTTGGCC 
ACAGATCCCT GGTCACTTAC ACTCATTCTC TTTTCTGTAC AATATGTATA TGGGAGTGGA 
AAGAAATACG TTGGTCCTTG TGGAGGAAGA GATTGCTCTG TTTGCCACTG TGTTCCTGAA 
AAGGGGTCTC GGGGTCCACC AGGACCACCA GGGCCACAGG GTCCAATTGG ACCCCTGGGA 
GCCCCAGGAC CCATTGGGCT TTCAGGAGAG AAAGGAATGA GAGGGGACCG CGGCCCTCCT 
GGAGCAGCAG GGGACAAAGG AGATAAGGGT TCAACTGGTG TTCCTGGATT TCCAGGTTTA 
GATGGCATAC CTGGGCACCC AGGGCCTCCT GGACCCAGAG GCAAACCTGG TATGAGTGGC 
CACAATGGCT CAAGAGGTGA CCCAGGGTTT CCAGGAGGAA GAGGAGCTCT TGGCCCAGGA 
GGCCCCCTAG GCCATCCTGG GGAAAAGGGA GAAAAAGGAA ATTCAGTGTT CATTTTAGGT 
GCCGTTAAAG GTATTCAGGG AGACAGAGGG GACCCAGGAC TGCCTGGCTT ACCAGGATCT 
TGGGGGGCAG GAGGACCGGC GGGTCCCACA GGATATCCTG GAGAGCCAGG GTTAGTGGGA 
CCTCCGGGCC AACCAGGGCG TCCAGGTTTG AAGGGAAATC CCGGTGTGGG AGTAAAGGGG 
CAAATGGGAG ACCCGGGTGA GGTTGGTCAG CAAGGTTCTC CTGGACCCAC CCTGTTGGTG 
GAGCCACCTG ACTTTCGTCT CTATAAAGGA GAAAAGGGTA TAAAAGGAAT TCCTGGAATG 
GTTGGACTGC CAGGACCACC AGGACGCAAG GGAGAATCTG ATATTGGGGC AAAAGGAGAA 
AAAGGTATTC CTGGATTTCC AGGGCCTCGG GGGGATCCTG GTTCCTATGG ATCTCCAGGT 
TTTCCAGGAT TAAAGGGAGA ACTAGGACTG GTTGGAGATC CTGGGCTATT TGGATTAATT 
GGCCCAAAGG GGGATCCTGG AAATCGAGGG CACCCAGGAC CACCAGGTGT TTTGGTGACT 
CCACCTCTTC CACTCAAAGG CCCACCAGGG GACCCAGGGT TCCCTGGCCG CTATGGAGAA 
ACAGGGGATG TTGGACCACC TGGTCCCCCA GGTCTCTTGG GCAGACCAGG GGAAGCCTGT 
GCAGGCATGA TAGGACCCCC TGGGCCACAA GGATTTCCTG GTCTTCCTGG GCTTCCAGGA 
GAAGCTGGTA TTCCTGGGAG ACCTGATTCT GCTCCAGGAA AACCAGGGAA GCCAGGATCA 
CCTGGCTTGC CTGGAGCACC AGGCCTGCAG GGCCTCCCAG GATCAAGTGT GATATACTGT 
AGTGTTGGGA ATCCTGGACC ACAAGGAATA AAAGGCAAAG TGGGTCCCCC AGGAGGAAGA 
GGCTCAAAAG GAGAAAAAGG AAATGAAGGA CTCTGTGCCT GTGAGCCTGG ACCCATGGGC 
CCCCCTGGCC CTCCAGGACT TCCTGGGAGG CAGGGGAGTA AGGGAGACTT GGGGCTCCCT 
GGCTGGCTTG GAACAAAAGG TGACCCAGGA CCTCCTGGTG CTGAAGGACC TCCAGGGCTA 
CCAGGAAAGC ATGGTGCCTC TGGACCACCT GGCAACAAAG GGGCGAAGGG TGACATGGTT 
GTATCAAGAG TTAAAGGGCA CAAAGGAGAA AGAGGTCCTG ATGGGCCCCC AGGATTTCCA 
GGGCAGCCAG GATCACATGG TCGGGATGGA CATGCTGGAG AAAAAGGGGA TCCAGGACCC 
CCAGGGGATC ATGAAGATGC GACCCCAGGT GGTAAAGGAT TTCCTGGACC TCTGGGCCCC 
CCGGGCAAAG CAGGACCTTT GGGGCCCCCA GGACTGGGAT TTCCTGGTCC ACCAGGACAG 
CGAGGCCACC CAGGAGTTCC AGGCCACCCA GGTGTGAGGG GCCCTGATGG CTTGAAGGGT 
CAGAAAGGTG ACACAATTTC TTGCAACGTA ACCTACCCTG GGAGGCACGG CCCTCCAGGT 
TTTGATGGAC CTCCAGGTCC GAAGGGATTT CCAGGTCCCC AAGGTGCCCC TGGGCTGAGT 
GGTTCAGATG GGCATAAAGG CAGACCTGGC ACACCAGGAA CAGCGGAAAT ACCAGGTCCA 
CCTGGTTTTC GTGGTGACAT GGGAGATCCG GGTTTTGGAG GTGAAAAGGG GTCCTCCCCT 
GCTGGGCCCC CAGGCCCTCC CGGCTCACCA GGAGTGAATG GTCAGAAAGG AATCCCGGGA 
GACCCTGCAT TTGGTCACCT GGGACCCCCG GGAAAGAGGG GTCTTTCAGG AGTGCCAGGG 
ATAAAAGGAC CCAGAGGTGA TCCGGGATGT CCAGGGGCTG AAGGGCCAGC TGGCATTCCT 
GGATTCCCAG GTCTCAAAGG TCCCAAAGGC AGAGAGGGAC ATGCTGGGTT TCCAGGTGTC 
CCAGGTCCAC CTGGCCATTC CTGTGAAAGA GGTGCTCCAG GGATACCAGG GCAACCGGGA 
CTCCCTGGGT ATCCAGGTAG CCCAGGTGCT CCAGGTGGGA AAGGACAGCT GGGAGATGTG 
GGGCCTCCCG GGCCAGCTGG AATGAAAGGC CTCCCTGGAC TCCCAGGATG GCCTGGGGCA 
CATGGTCCCC CAGGCCTCCC AGGAATCCCA GGTCCCTTTG GAGATGATGG GCTACCTGGT 
CCTCCAGGTC CAAAGGGACC CCAGGGGCTG CCTGGTTTCC CAGGTTTTCC CGGAGAAAGA 
GGAAAGCCTG GTGCAGAGGG ATGTCCTGGC ACAAAGGGAG AACCTGGAGA GAAGGGCATG 
TCTGGCCTTC CCGGAGACCG GGGACTGAGA GGGGCCAAAG GAGCCATAGG ACCTCCCGGA 
GATGAAGGAG AAATGGCTAT CATTTCCCAA AAGGGAACAC CTGGGGAACC TGGACCTCCT 
GGAGATGATG GATTCCCAGG AGAAAGAGGT GATAAAGGAA CTCCCGGGAT GCAAGGGAGA 
AGAGGAGAGC CGGGAAGATA CGGACCACCT GGATTTCAGA GAGGGGAACC TGGTGAGAAA 
GGTCAGCCAG GGCATCCTGG ACCCCCAGGC CCTCCAGGCT CAACTGGTCT AAGAGGGTTC 
ATTGGTTTTC CAGGACTTCC AGGTGACCAG GGTGAGCCAG GTTCTCCAGG TCCCCCTGGA 
TTTTCAGGAA TTGATGGAGC AAGAGGACCT AAAGGAAACA AAGGTGACCC TGCCAGTCAC 
TTTGGTCCAC CTGGTCCAAA GGGTGAGCCA GGTAGCCCTG GATGTCCAGG GCATTTTGGA 
GCATCTGGAG AGCAGGGCTT GCCTGGTATT CAAGGGCCCA GAGGATCACC TGGAAGGCCA 
GGGCCACCTG GCTCCTCTGG ACCACCAGGG TGCCCAGGTG ATCACGGGAT GCCTGGGCTG 
AGGGGACAGC CAGGAGAAAT GGGAGACCCT GGGCCAAGAG GCCTCCAGGG GGATCCAGGG 
ATACCAGGTC CTCCGGGAAT AAAAGGTCCC TCCGGATCAC CTGGCCTGAA CGGCTTGCAT 
GGATTGAAAG GTCAGAAAGG AACTAAAGGT GCTTCAGGTT TGCATGATGT GGGGCCACCT 
GGTCCAGTGG GAATACCTGG GCTAAAAGGG GAGAGAGGAG ACCCTGGGAG CCCAGGAATC 
TCTCCTCCAG GTCCTCGTGG AGAAAAAGGT CCCCCAGGAC CCCCAGGGAG TTCAGGACCA 
CCTGGTCCTG CAGGTGCCAC AGGAAGAGCT CCTAAGGACA TTCCTGACCC GGGTCCACCT 
GGAGATCAGG GACCTCCTGG TCCTGATGGC CCAAGAGGAG CACCTGGGCC TCCAGGCCTC 
CCTGGGAGTG TTGACCTTCT GAGAGGGGAG CCAGGTGACT GTGGTCTACC AGGGCCACCA 
GGTCCCCCTG GCCCACCAGG CCCTCCAGGA TACAAAGGCT TTCCAGGATG TGATGGAAAA 
GATGGCCAGA AAGGACCAAT GGGATTCCCG GGACCGCAGG GACCACATGG ATTTCCTGGG 
CCACCTGGAG AGAAGGGTTT ACCTGGACCT CCAGGGAGAA AAGGGCCCAC TGGTCTTCCA 
GGTCCCAGAG GTGAACCGGG GCCACCTGCA GATGTGGATG ACTGTCCCCG AATCCCAGGC 
CTTCCTGGGG CGCCAGGCAT GAGAGGACCA GAAGGAGCCA TGGGGCTCTC TGGAATGAGA 
GGCCCCCCAG GACCAGGGTG CAAAGGAGAG CCTGGGCTGG ATGGCAGGAG GGGTGTGGAT 
GGCGTCCCTG GGTCTCCTGG GCCTCCCGGA CGTAAAGGTG ACACAGGAGA AGACGGCTAC 
CCTGGAGGAC CAGGGCCTCC TGGTCCCATT GGGGATCCTG GGCCCAAAGG GTTTGGCCCT 
GGATACCTCG GTGGCTTCCT CCTGGTTCTC CACAGTCAGA CGGACCAGGA GCCCACCTGC 
CCCCTGGGCA TGCCCAGGCT CTGGACTGGG TATAGTCTGT TATACCTGGA AGGGCAAGAG 
AAAGCTCACA ATCAAGACCT TGGTCTGGCA GGGTCTTGCC TTCCCGTGTT TAGCACGCTG 
CCCTTTGCCT ACTGCAACAT CCACCAGGTG TGCCACTATG CCCAGAGAAA CGACAGATCC 
TACTGGCTGG CCAGCGCTGC GCCCCTCCCC ATGATGCCAC TCTCTGAAGA GGCGATCCGC 
CCCTATGTCA GCCGCTGTGC GGTATGCGAG GCCCCGGCCC AGGCGGTGGC GGTGCACAGC 
CAGGACCAGT CCATCCCCCC ATGTCCGCAG ACCTGGAGGA GCCTCTGGAT CGGGTATTCA 
TTCCTGATGC ACACAGGAGC TGGGGACCAA GGAGGAGGGC AGGCCCTTAT GTCACCTGGC 
AGCTGCCTGG AAGATTTCAG AGCAGCACCA TTCCTTGAAT GCCAGGGCCG GCAGGGAACT 
TGCCACTTTT TTGCAAATAA GTATAGCTTC TGGCTCACAA CGGTGAAAGC AGACTTGCAG 
TTTTCCTCTG CTCCAGCACC AGACACCTTA AAAGAAAGCC AGGCCCAACG CCAGAAAATC 
AGCCGGTGCC AGGTCTGCGT GAAGTATAGC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009442740.3
CDS	533..5605
Translation	MWSLHIVLMRCSFRLTKSLATDPWSLTLILFSVQYVYGSGKKYVGPCGGRDCSVCHCVPEKGSRGPPGPPGPQGPIGPLGAPGPIGLSGEKGMRGDRGPPGAAGDKGDKGSTGVPGFPGLDGIPGHPGPPGPRGKPGMSGHNGSRGDPGFPGGRGALGPGGPLGHPGEKGEKGNSVFILGAVKGIQGDRGDPGLPGLPGSWGAGGPAGPTGYPGEPGLVGPPGQPGRPGLKGNPGVGVKGQMGDPGEVGQQGSPGPTLLVEPPDFRLYKGEKGIKGIPGMVGLPGPPGRKGESDIGAKGEKGIPGFPGPRGDPGSYGSPGFPGLKGELGLVGDPGLFGLIGPKGDPGNRGHPGPPGVLVTPPLPLKGPPGDPGFPGRYGETGDVGPPGPPGLLGRPGEACAGMIGPPGPQGFPGLPGLPGEAGIPGRPDSAPGKPGKPGSPGLPGAPGLQGLPGSSVIYCSVGNPGPQGIKGKVGPPGGRGSKGEKGNEGLCACEPGPMGPPGPPGLPGRQGSKGDLGLPGWLGTKGDPGPPGAEGPPGLPGKHGASGPPGNKGAKGDMVVSRVKGHKGERGPDGPPGFPGQPGSHGRDGHAGEKGDPGPPGDHEDATPGGKGFPGPLGPPGKAGPLGPPGLGFPGPPGQRGHPGVPGHPGVRGPDGLKGQKGDTISCNVTYPGRHGPPGFDGPPGPKGFPGPQGAPGLSGSDGHKGRPGTPGTAEIPGPPGFRGDMGDPGFGGEKGSSPAGPPGPPGSPGVNGQKGIPGDPAFGHLGPPGKRGLSGVPGIKGPRGDPGCPGAEGPAGIPGFPGLKGPKGREGHAGFPGVPGPPGHSCERGAPGIPGQPGLPGYPGSPGAPGGKGQLGDVGPPGPAGMKGLPGLPGWPGAHGPPGLPGIPGPFGDDGLPGPPGPKGPQGLPGFPGFPGERGKPGAEGCPGTKGEPGEKGMSGLPGDRGLRGAKGAIGPPGDEGEMAIISQKGTPGEPGPPGDDGFPGERGDKGTPGMQGRRGEPGRYGPPGFQRGEPGEKGQPGHPGPPGPPGSTGLRGFIGFPGLPGDQGEPGSPGPPGFSGIDGARGPKGNKGDPASHFGPPGPKGEPGSPGCPGHFGASGEQGLPGIQGPRGSPGRPGPPGSSGPPGCPGDHGMPGLRGQPGEMGDPGPRGLQGDPGIPGPPGIKGPSGSPGLNGLHGLKGQKGTKGASGLHDVGPPGPVGIPGLKGERGDPGSPGISPPGPRGEKGPPGPPGSSGPPGPAGATGRAPKDIPDPGPPGDQGPPGPDGPRGAPGPPGLPGSVDLLRGEPGDCGLPGPPGPPGPPGPPGYKGFPGCDGKDGQKGPMGFPGPQGPHGFPGPPGEKGLPGPPGRKGPTGLPGPRGEPGPPADVDDCPRIPGLPGAPGMRGPEGAMGLSGMRGPPGPGCKGEPGLDGRRGVDGVPGSPGPPGRKGDTGEDGYPGGPGPPGPIGDPGPKGFGPGYLGGFLLVLHSQTDQEPTCPLGMPRLWTGYSLLYLEGQEKAHNQDLGLAGSCLPVFSTLPFAYCNIHQVCHYAQRNDRSYWLASAAPLPMMPLSEEAIRPYVSRCAVCEAPAQAVAVHSQDQSIPPCPQTWRSLWIGYSFLMHTGAGDQGGGQALMSPGSCLEDFRAAPFLECQGRQGTCHFFANKYSFWLTTVKADLQFSSAPAPDTLKESQAQRQKISRCQVCVKYS

Target ORF information:

RefSeq Version	XM_009444465.3
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes collagen type IV alpha 4 chain (COL4A4), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009444465.3

ORF Insert Sequence:

ATGTGGTCTC TGCACATAGT ACTAATGAGG TGCTCCTTCA GATTGACCAA GTCCTTGGCC 
ACAGATCCCT GGTCACTTAC ACTCATTCTC TTTTCTGTAC AATATGTATA TGGGAGTGGA 
AAGAAATACG TTGGTCCTTG TGGAGGAAGA GATTGCTCTG TTTGCCACTG TGTTCCTGAA 
AAGGGGTCTC GGGGTCCACC AGGACCACCA GGGCCACAGG GTCCAATTGG ACCCCTGGGA 
GCCCCAGGAC CCATTGGGCT TTCAGGAGAG AAAGGAATGA GAGGGGACCG CGGCCCTCCT 
GGAGCAGCAG GGGACAAAGG AGATAAGGGT TCAACTGGTG TTCCTGGATT TCCAGGTTTA 
GATGGCATAC CTGGGCACCC AGGGCCTCCT GGACCCAGAG GCAAACCTGG TATGAGTGGC 
CACAATGGCT CAAGAGGTGA CCCAGGGTTT CCAGGAGGAA GAGGAGCTCT TGGCCCAGGA 
GGCCCCCTAG GCCATCCTGG GGAAAAGGGA GAAAAAGGAA ATTCAGTGTT CATTTTAGGT 
GCCGTTAAAG GTATTCAGGG AGACAGAGGG GACCCAGGAC TGCCTGGCTT ACCAGGATCT 
TGGGGGGCAG GAGGACCGGC GGGTCCCACA GGATATCCTG GAGAGCCAGG GTTAGTGGGA 
CCTCCGGGCC AACCAGGGCG TCCAGGTTTG AAGGGAAATC CCGGTGTGGG AGTAAAGGGG 
CAAATGGGAG ACCCGGGTGA GGTTGGTCAG CAAGGTTCTC CTGGACCCAC CCTGTTGGTG 
GAGCCACCTG ACTTTCGTCT CTATAAAGGA GAAAAGGGTA TAAAAGGAAT TCCTGGAATG 
GTTGGACTGC CAGGACCACC AGGACGCAAG GGAGAATCTG ATATTGGGGC AAAAGGAGAA 
AAAGGTATTC CTGGATTTCC AGGGCCTCGG GGGGATCCTG GTTCCTATGG ATCTCCAGGT 
TTTCCAGGAT TAAAGGGAGA ACTAGGACTG GTTGGAGATC CTGGGCTATT TGGATTAATT 
GGCCCAAAGG GGGATCCTGG AAATCGAGGG CACCCAGGAC CACCAGGTGT TTTGGTGACT 
CCACCTCTTC CACTCAAAGG CCCACCAGGG GACCCAGGGT TCCCTGGCCG CTATGGAGAA 
ACAGGGGATG TTGGACCACC TGGTCCCCCA GGTCTCTTGG GCAGACCAGG GGAAGCCTGT 
GCAGGCATGA TAGGACCCCC TGGGCCACAA GGATTTCCTG GTCTTCCTGG GCTTCCAGGA 
GAAGCTGGTA TTCCTGGGAG ACCTGATTCT GCTCCAGGAA AACCAGGGAA GCCAGGATCA 
CCTGGCTTGC CTGGAGCACC AGGCCTGCAG GGCCTCCCAG GATCAAGTGT GATATACTGT 
AGTGTTGGGA ATCCTGGACC ACAAGGAATA AAAGGCAAAG TGGGTCCCCC AGGAGGAAGA 
GGCTCAAAAG GAGAAAAAGG AAATGAAGGA CTCTGTGCCT GTGAGCCTGG ACCCATGGGC 
CCCCCTGGCC CTCCAGGACT TCCTGGGAGG CAGGGGAGTA AGGGAGACTT GGGGCTCCCT 
GGCTGGCTTG GAACAAAAGG TGACCCAGGA CCTCCTGGTG CTGAAGGACC TCCAGGGCTA 
CCAGGAAAGC ATGGTGCCTC TGGACCACCT GGCAACAAAG GGGCGAAGGG TGACATGGTT 
GTATCAAGAG TTAAAGGGCA CAAAGGAGAA AGAGGTCCTG ATGGGCCCCC AGGATTTCCA 
GGGCAGCCAG GATCACATGG TCGGGATGGA CATGCTGGAG AAAAAGGGGA TCCAGGACCC 
CCAGGGGATC ATGAAGATGC GACCCCAGGT GGTAAAGGAT TTCCTGGACC TCTGGGCCCC 
CCGGGCAAAG CAGGACCTTT GGGGCCCCCA GGACTGGGAT TTCCTGGTCC ACCAGGACAG 
CGAGGCCACC CAGGAGTTCC AGGCCACCCA GGTGTGAGGG GCCCTGATGG CTTGAAGGGT 
CAGAAAGGTG ACACAATTTC TTGCAACGTA ACCTACCCTG GGAGGCACGG CCCTCCAGGT 
TTTGATGGAC CTCCAGGTCC GAAGGGATTT CCAGGTCCCC AAGGTGCCCC TGGGCTGAGT 
GGTTCAGATG GGCATAAAGG CAGACCTGGC ACACCAGGAA CAGCGGAAAT ACCAGGTCCA 
CCTGGTTTTC GTGGTGACAT GGGAGATCCG GGTTTTGGAG GTGAAAAGGG GTCCTCCCCT 
GCTGGGCCCC CAGGCCCTCC CGGCTCACCA GGAGTGAATG GTCAGAAAGG AATCCCGGGA 
GACCCTGCAT TTGGTCACCT GGGACCCCCG GGAAAGAGGG GTCTTTCAGG AGTGCCAGGG 
ATAAAAGGAC CCAGAGGTGA TCCGGGATGT CCAGGGGCTG AAGGGCCAGC TGGCATTCCT 
GGATTCCCAG GTCTCAAAGG TCCCAAAGGC AGAGAGGGAC ATGCTGGGTT TCCAGGTGTC 
CCAGGTCCAC CTGGCCATTC CTGTGAAAGA GGTGCTCCAG GGATACCAGG GCAACCGGGA 
CTCCCTGGGT ATCCAGGTAG CCCAGGTGCT CCAGGTGGGA AAGGACAGCT GGGAGATGTG 
GGGCCTCCCG GGCCAGCTGG AATGAAAGGC CTCCCTGGAC TCCCAGGATG GCCTGGGGCA 
CATGGTCCCC CAGGCCTCCC AGGAATCCCA GGTCCCTTTG GAGATGATGG GCTACCTGGT 
CCTCCAGGTC CAAAGGGACC CCAGGGGCTG CCTGGTTTCC CAGGTTTTCC CGGAGAAAGA 
GGAAAGCCTG GTGCAGAGGG ATGTCCTGGC ACAAAGGGAG AACCTGGAGA GAAGGGCATG 
TCTGGCCTTC CCGGAGACCG GGGACTGAGA GGGGCCAAAG GAGCCATAGG ACCTCCCGGA 
GATGAAGGAG AAATGGCTAT CATTTCCCAA AAGGGAACAC CTGGGGAACC TGGACCTCCT 
GGAGATGATG GATTCCCAGG AGAAAGAGGT GATAAAGGAA CTCCCGGGAT GCAAGGGAGA 
AGAGGAGAGC CGGGAAGATA CGGACCACCT GGATTTCAGA GAGGGGAACC TGGTGAGAAA 
GGTCAGCCAG GGCATCCTGG ACCCCCAGGC CCTCCAGGCT CAACTGGTCT AAGAGGGTTC 
ATTGGTTTTC CAGGACTTCC AGGTGACCAG GGTGAGCCAG GTTCTCCAGG TCCCCCTGGA 
TTTTCAGGAA TTGATGGAGC AAGAGGACCT AAAGGAAACA AAGGTGACCC TGCCAGTCAC 
TTTGGTCCAC CTGGTCCAAA GGGTGAGCCA GGTAGCCCTG GATGTCCAGG GCATTTTGGA 
GCATCTGGAG AGCAGGGCTT GCCTGGTATT CAAGGGCCCA GAGGATCACC TGGAAGGCCA 
GGGCCACCTG GCTCCTCTGG ACCACCAGGG TGCCCAGGTG ATCACGGGAT GCCTGGGCTG 
AGGGGACAGC CAGGAGAAAT GGGAGACCCT GGGCCAAGAG GCCTCCAGGG GGATCCAGGG 
ATACCAGGTC CTCCGGGAAT AAAAGGTCCC TCCGGATCAC CTGGCCTGAA CGGCTTGCAT 
GGATTGAAAG GTCAGAAAGG AACTAAAGGT GCTTCAGGTT TGCATGATGT GGGGCCACCT 
GGTCCAGTGG GAATACCTGG GCTAAAAGGG GAGAGAGGAG ACCCTGGGAG CCCAGGAATC 
TCTCCTCCAG GTCCTCGTGG AGAAAAAGGT CCCCCAGGAC CCCCAGGGAG TTCAGGACCA 
CCTGGTCCTG CAGGTGCCAC AGGAAGAGCT CCTAAGGACA TTCCTGACCC GGGTCCACCT 
GGAGATCAGG GACCTCCTGG TCCTGATGGC CCAAGAGGAG CACCTGGGCC TCCAGGCCTC 
CCTGGGAGTG TTGACCTTCT GAGAGGGGAG CCAGGTGACT GTGGTCTACC AGGGCCACCA 
GGTCCCCCTG GCCCACCAGG CCCTCCAGGA TACAAAGGCT TTCCAGGATG TGATGGAAAA 
GATGGCCAGA AAGGACCAAT GGGATTCCCG GGACCGCAGG GACCACATGG ATTTCCTGGG 
CCACCTGGAG AGAAGGGTTT ACCTGGACCT CCAGGGAGAA AAGGGCCCAC TGGTCTTCCA 
GGTCCCAGAG GTGAACCGGG GCCACCTGCA GATGTGGATG ACTGTCCCCG AATCCCAGGC 
CTTCCTGGGG CGCCAGGCAT GAGAGGACCA GAAGGAGCCA TGGGGCTCTC TGGAATGAGA 
GGCCCCCCAG GACCAGGGTG CAAAGGAGAG CCTGGGCTGG ATGGCAGGAG GGGTGTGGAT 
GGCGTCCCTG GGTCTCCTGG GCCTCCCGGA CGTAAAGGTG ACACAGGAGA AGACGGCTAC 
CCTGGAGGAC CAGGGCCTCC TGGTCCCATT GGGGATCCTG GGCCCAAAGG GTTTGGCCCT 
GGATACCTCG GTGGCTTCCT CCTGGTTCTC CACAGTCAGA CGGACCAGGA GCCCACCTGC 
CCCCTGGGCA TGCCCAGGCT CTGGACTGGG TATAGTCTGT TATACCTGGA AGGGCAAGAG 
AAAGCTCACA ATCAAGACCT TGGTCTGGCA GGGTCTTGCC TTCCCGTGTT TAGCACGCTG 
CCCTTTGCCT ACTGCAACAT CCACCAGGTG TGCCACTATG CCCAGAGAAA CGACAGATCC 
TACTGGCTGG CCAGCGCTGC GCCCCTCCCC ATGATGCCAC TCTCTGAAGA GGCGATCCGC 
CCCTATGTCA GCCGCTGTGC GGTATGCGAG GCCCCGGCCC AGGCGGTGGC GGTGCACAGC 
CAGGACCAGT CCATCCCCCC ATGTCCGCAG ACCTGGAGGA GCCTCTGGAT CGGGTATTCA 
TTCCTGATGC ACACAGGAGC TGGGGACCAA GGAGGAGGGC AGGCCCTTAT GTCACCTGGC 
AGCTGCCTGG AAGATTTCAG AGCAGCACCA TTCCTTGAAT GCCAGGGCCG GCAGGGAACT 
TGCCACTTTT TTGCAAATAA GTATAGCTTC TGGCTCACAA CGGTGAAAGC AGACTTGCAG 
TTTTCCTCTG CTCCAGCACC AGACACCTTA AAAGAAAGCC AGGCCCAACG CCAGAAAATC 
AGCCGGTGCC AGGTCTGCGT GAAGTATAGC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL4A4 ( XM_516128.5 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_516128.4 Pan troglodytes collagen type IV alpha 4 chain (COL4A4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OPc104269
Clone ID Related Accession (Same CDS sequence)	XM_009444465.3 , XM_516128.5 , XM_009444464.3 , XM_016950591.2
Accession Version	XM_516128.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5073bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	collagen alpha-4(IV) chain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036881.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_516128.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGTCTC TGCACATAGT ACTAATGAGG TGCTCCTTCA GATTGACCAA GTCCTTGGCC 
ACAGATCCCT GGTCACTTAC ACTCATTCTC TTTTCTGTAC AATATGTATA TGGGAGTGGA 
AAGAAATACG TTGGTCCTTG TGGAGGAAGA GATTGCTCTG TTTGCCACTG TGTTCCTGAA 
AAGGGGTCTC GGGGTCCACC AGGACCACCA GGGCCACAGG GTCCAATTGG ACCCCTGGGA 
GCCCCAGGAC CCATTGGGCT TTCAGGAGAG AAAGGAATGA GAGGGGACCG CGGCCCTCCT 
GGAGCAGCAG GGGACAAAGG AGATAAGGGT TCAACTGGTG TTCCTGGATT TCCAGGTTTA 
GATGGCATAC CTGGGCACCC AGGGCCTCCT GGACCCAGAG GCAAACCTGG TATGAGTGGC 
CACAATGGCT CAAGAGGTGA CCCAGGGTTT CCAGGAGGAA GAGGAGCTCT TGGCCCAGGA 
GGCCCCCTAG GCCATCCTGG GGAAAAGGGA GAAAAAGGAA ATTCAGTGTT CATTTTAGGT 
GCCGTTAAAG GTATTCAGGG AGACAGAGGG GACCCAGGAC TGCCTGGCTT ACCAGGATCT 
TGGGGGGCAG GAGGACCGGC GGGTCCCACA GGATATCCTG GAGAGCCAGG GTTAGTGGGA 
CCTCCGGGCC AACCAGGGCG TCCAGGTTTG AAGGGAAATC CCGGTGTGGG AGTAAAGGGG 
CAAATGGGAG ACCCGGGTGA GGTTGGTCAG CAAGGTTCTC CTGGACCCAC CCTGTTGGTG 
GAGCCACCTG ACTTTCGTCT CTATAAAGGA GAAAAGGGTA TAAAAGGAAT TCCTGGAATG 
GTTGGACTGC CAGGACCACC AGGACGCAAG GGAGAATCTG ATATTGGGGC AAAAGGAGAA 
AAAGGTATTC CTGGATTTCC AGGGCCTCGG GGGGATCCTG GTTCCTATGG ATCTCCAGGT 
TTTCCAGGAT TAAAGGGAGA ACTAGGACTG GTTGGAGATC CTGGGCTATT TGGATTAATT 
GGCCCAAAGG GGGATCCTGG AAATCGAGGG CACCCAGGAC CACCAGGTGT TTTGGTGACT 
CCACCTCTTC CACTCAAAGG CCCACCAGGG GACCCAGGGT TCCCTGGCCG CTATGGAGAA 
ACAGGGGATG TTGGACCACC TGGTCCCCCA GGTCTCTTGG GCAGACCAGG GGAAGCCTGT 
GCAGGCATGA TAGGACCCCC TGGGCCACAA GGATTTCCTG GTCTTCCTGG GCTTCCAGGA 
GAAGCTGGTA TTCCTGGGAG ACCTGATTCT GCTCCAGGAA AACCAGGGAA GCCAGGATCA 
CCTGGCTTGC CTGGAGCACC AGGCCTGCAG GGCCTCCCAG GATCAAGTGT GATATACTGT 
AGTGTTGGGA ATCCTGGACC ACAAGGAATA AAAGGCAAAG TGGGTCCCCC AGGAGGAAGA 
GGCTCAAAAG GAGAAAAAGG AAATGAAGGA CTCTGTGCCT GTGAGCCTGG ACCCATGGGC 
CCCCCTGGCC CTCCAGGACT TCCTGGGAGG CAGGGGAGTA AGGGAGACTT GGGGCTCCCT 
GGCTGGCTTG GAACAAAAGG TGACCCAGGA CCTCCTGGTG CTGAAGGACC TCCAGGGCTA 
CCAGGAAAGC ATGGTGCCTC TGGACCACCT GGCAACAAAG GGGCGAAGGG TGACATGGTT 
GTATCAAGAG TTAAAGGGCA CAAAGGAGAA AGAGGTCCTG ATGGGCCCCC AGGATTTCCA 
GGGCAGCCAG GATCACATGG TCGGGATGGA CATGCTGGAG AAAAAGGGGA TCCAGGACCC 
CCAGGGGATC ATGAAGATGC GACCCCAGGT GGTAAAGGAT TTCCTGGACC TCTGGGCCCC 
CCGGGCAAAG CAGGACCTTT GGGGCCCCCA GGACTGGGAT TTCCTGGTCC ACCAGGACAG 
CGAGGCCACC CAGGAGTTCC AGGCCACCCA GGTGTGAGGG GCCCTGATGG CTTGAAGGGT 
CAGAAAGGTG ACACAATTTC TTGCAACGTA ACCTACCCTG GGAGGCACGG CCCTCCAGGT 
TTTGATGGAC CTCCAGGTCC GAAGGGATTT CCAGGTCCCC AAGGTGCCCC TGGGCTGAGT 
GGTTCAGATG GGCATAAAGG CAGACCTGGC ACACCAGGAA CAGCGGAAAT ACCAGGTCCA 
CCTGGTTTTC GTGGTGACAT GGGAGATCCG GGTTTTGGAG GTGAAAAGGG GTCCTCCCCT 
GCTGGGCCCC CAGGCCCTCC CGGCTCACCA GGAGTGAATG GTCAGAAAGG AATCCCGGGA 
GACCCTGCAT TTGGTCACCT GGGACCCCCG GGAAAGAGGG GTCTTTCAGG AGTGCCAGGG 
ATAAAAGGAC CCAGAGGTGA TCCGGGATGT CCAGGGGCTG AAGGGCCAGC TGGCATTCCT 
GGATTCCCAG GTCTCAAAGG TCCCAAAGGC AGAGAGGGAC ATGCTGGGTT TCCAGGTGTC 
CCAGGTCCAC CTGGCCATTC CTGTGAAAGA GGTGCTCCAG GGATACCAGG GCAACCGGGA 
CTCCCTGGGT ATCCAGGTAG CCCAGGTGCT CCAGGTGGGA AAGGACAGCT GGGAGATGTG 
GGGCCTCCCG GGCCAGCTGG AATGAAAGGC CTCCCTGGAC TCCCAGGATG GCCTGGGGCA 
CATGGTCCCC CAGGCCTCCC AGGAATCCCA GGTCCCTTTG GAGATGATGG GCTACCTGGT 
CCTCCAGGTC CAAAGGGACC CCAGGGGCTG CCTGGTTTCC CAGGTTTTCC CGGAGAAAGA 
GGAAAGCCTG GTGCAGAGGG ATGTCCTGGC ACAAAGGGAG AACCTGGAGA GAAGGGCATG 
TCTGGCCTTC CCGGAGACCG GGGACTGAGA GGGGCCAAAG GAGCCATAGG ACCTCCCGGA 
GATGAAGGAG AAATGGCTAT CATTTCCCAA AAGGGAACAC CTGGGGAACC TGGACCTCCT 
GGAGATGATG GATTCCCAGG AGAAAGAGGT GATAAAGGAA CTCCCGGGAT GCAAGGGAGA 
AGAGGAGAGC CGGGAAGATA CGGACCACCT GGATTTCAGA GAGGGGAACC TGGTGAGAAA 
GGTCAGCCAG GGCATCCTGG ACCCCCAGGC CCTCCAGGCT CAACTGGTCT AAGAGGGTTC 
ATTGGTTTTC CAGGACTTCC AGGTGACCAG GGTGAGCCAG GTTCTCCAGG TCCCCCTGGA 
TTTTCAGGAA TTGATGGAGC AAGAGGACCT AAAGGAAACA AAGGTGACCC TGCCAGTCAC 
TTTGGTCCAC CTGGTCCAAA GGGTGAGCCA GGTAGCCCTG GATGTCCAGG GCATTTTGGA 
GCATCTGGAG AGCAGGGCTT GCCTGGTATT CAAGGGCCCA GAGGATCACC TGGAAGGCCA 
GGGCCACCTG GCTCCTCTGG ACCACCAGGG TGCCCAGGTG ATCACGGGAT GCCTGGGCTG 
AGGGGACAGC CAGGAGAAAT GGGAGACCCT GGGCCAAGAG GCCTCCAGGG GGATCCAGGG 
ATACCAGGTC CTCCGGGAAT AAAAGGTCCC TCCGGATCAC CTGGCCTGAA CGGCTTGCAT 
GGATTGAAAG GTCAGAAAGG AACTAAAGGT GCTTCAGGTT TGCATGATGT GGGGCCACCT 
GGTCCAGTGG GAATACCTGG GCTAAAAGGG GAGAGAGGAG ACCCTGGGAG CCCAGGAATC 
TCTCCTCCAG GTCCTCGTGG AGAAAAAGGT CCCCCAGGAC CCCCAGGGAG TTCAGGACCA 
CCTGGTCCTG CAGGTGCCAC AGGAAGAGCT CCTAAGGACA TTCCTGACCC GGGTCCACCT 
GGAGATCAGG GACCTCCTGG TCCTGATGGC CCAAGAGGAG CACCTGGGCC TCCAGGCCTC 
CCTGGGAGTG TTGACCTTCT GAGAGGGGAG CCAGGTGACT GTGGTCTACC AGGGCCACCA 
GGTCCCCCTG GCCCACCAGG CCCTCCAGGA TACAAAGGCT TTCCAGGATG TGATGGAAAA 
GATGGCCAGA AAGGACCAAT GGGATTCCCG GGACCGCAGG GACCACATGG ATTTCCTGGG 
CCACCTGGAG AGAAGGGTTT ACCTGGACCT CCAGGGAGAA AAGGGCCCAC TGGTCTTCCA 
GGTCCCAGAG GTGAACCGGG GCCACCTGCA GATGTGGATG ACTGTCCCCG AATCCCAGGC 
CTTCCTGGGG CGCCAGGCAT GAGAGGACCA GAAGGAGCCA TGGGGCTCTC TGGAATGAGA 
GGCCCCCCAG GACCAGGGTG CAAAGGAGAG CCTGGGCTGG ATGGCAGGAG GGGTGTGGAT 
GGCGTCCCTG GGTCTCCTGG GCCTCCCGGA CGTAAAGGTG ACACAGGAGA AGACGGCTAC 
CCTGGAGGAC CAGGGCCTCC TGGTCCCATT GGGGATCCTG GGCCCAAAGG GTTTGGCCCT 
GGATACCTCG GTGGCTTCCT CCTGGTTCTC CACAGTCAGA CGGACCAGGA GCCCACCTGC 
CCCCTGGGCA TGCCCAGGCT CTGGACTGGG TATAGTCTGT TATACCTGGA AGGGCAAGAG 
AAAGCTCACA ATCAAGACCT TGGTCTGGCA GGGTCTTGCC TTCCCGTGTT TAGCACGCTG 
CCCTTTGCCT ACTGCAACAT CCACCAGGTG TGCCACTATG CCCAGAGAAA CGACAGATCC 
TACTGGCTGG CCAGCGCTGC GCCCCTCCCC ATGATGCCAC TCTCTGAAGA GGCGATCCGC 
CCCTATGTCA GCCGCTGTGC GGTATGCGAG GCCCCGGCCC AGGCGGTGGC GGTGCACAGC 
CAGGACCAGT CCATCCCCCC ATGTCCGCAG ACCTGGAGGA GCCTCTGGAT CGGGTATTCA 
TTCCTGATGC ACACAGGAGC TGGGGACCAA GGAGGAGGGC AGGCCCTTAT GTCACCTGGC 
AGCTGCCTGG AAGATTTCAG AGCAGCACCA TTCCTTGAAT GCCAGGGCCG GCAGGGAACT 
TGCCACTTTT TTGCAAATAA GTATAGCTTC TGGCTCACAA CGGTGAAAGC AGACTTGCAG 
TTTTCCTCTG CTCCAGCACC AGACACCTTA AAAGAAAGCC AGGCCCAACG CCAGAAAATC 
AGCCGGTGCC AGGTCTGCGT GAAGTATAGC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_516128.4
CDS	656..5728
Translation	MWSLHIVLMRCSFRLTKSLATDPWSLTLILFSVQYVYGSGKKYVGPCGGRDCSVCHCVPEKGSRGPPGPPGPQGPIGPLGAPGPIGLSGEKGMRGDRGPPGAAGDKGDKGSTGVPGFPGLDGIPGHPGPPGPRGKPGMSGHNGSRGDPGFPGGRGALGPGGPLGHPGEKGEKGNSVFILGAVKGIQGDRGDPGLPGLPGSWGAGGPAGPTGYPGEPGLVGPPGQPGRPGLKGNPGVGVKGQMGDPGEVGQQGSPGPTLLVEPPDFRLYKGEKGIKGIPGMVGLPGPPGRKGESDIGAKGEKGIPGFPGPRGDPGSYGSPGFPGLKGELGLVGDPGLFGLIGPKGDPGNRGHPGPPGVLVTPPLPLKGPPGDPGFPGRYGETGDVGPPGPPGLLGRPGEACAGMIGPPGPQGFPGLPGLPGEAGIPGRPDSAPGKPGKPGSPGLPGAPGLQGLPGSSVIYCSVGNPGPQGIKGKVGPPGGRGSKGEKGNEGLCACEPGPMGPPGPPGLPGRQGSKGDLGLPGWLGTKGDPGPPGAEGPPGLPGKHGASGPPGNKGAKGDMVVSRVKGHKGERGPDGPPGFPGQPGSHGRDGHAGEKGDPGPPGDHEDATPGGKGFPGPLGPPGKAGPLGPPGLGFPGPPGQRGHPGVPGHPGVRGPDGLKGQKGDTISCNVTYPGRHGPPGFDGPPGPKGFPGPQGAPGLSGSDGHKGRPGTPGTAEIPGPPGFRGDMGDPGFGGEKGSSPAGPPGPPGSPGVNGQKGIPGDPAFGHLGPPGKRGLSGVPGIKGPRGDPGCPGAEGPAGIPGFPGLKGPKGREGHAGFPGVPGPPGHSCERGAPGIPGQPGLPGYPGSPGAPGGKGQLGDVGPPGPAGMKGLPGLPGWPGAHGPPGLPGIPGPFGDDGLPGPPGPKGPQGLPGFPGFPGERGKPGAEGCPGTKGEPGEKGMSGLPGDRGLRGAKGAIGPPGDEGEMAIISQKGTPGEPGPPGDDGFPGERGDKGTPGMQGRRGEPGRYGPPGFQRGEPGEKGQPGHPGPPGPPGSTGLRGFIGFPGLPGDQGEPGSPGPPGFSGIDGARGPKGNKGDPASHFGPPGPKGEPGSPGCPGHFGASGEQGLPGIQGPRGSPGRPGPPGSSGPPGCPGDHGMPGLRGQPGEMGDPGPRGLQGDPGIPGPPGIKGPSGSPGLNGLHGLKGQKGTKGASGLHDVGPPGPVGIPGLKGERGDPGSPGISPPGPRGEKGPPGPPGSSGPPGPAGATGRAPKDIPDPGPPGDQGPPGPDGPRGAPGPPGLPGSVDLLRGEPGDCGLPGPPGPPGPPGPPGYKGFPGCDGKDGQKGPMGFPGPQGPHGFPGPPGEKGLPGPPGRKGPTGLPGPRGEPGPPADVDDCPRIPGLPGAPGMRGPEGAMGLSGMRGPPGPGCKGEPGLDGRRGVDGVPGSPGPPGRKGDTGEDGYPGGPGPPGPIGDPGPKGFGPGYLGGFLLVLHSQTDQEPTCPLGMPRLWTGYSLLYLEGQEKAHNQDLGLAGSCLPVFSTLPFAYCNIHQVCHYAQRNDRSYWLASAAPLPMMPLSEEAIRPYVSRCAVCEAPAQAVAVHSQDQSIPPCPQTWRSLWIGYSFLMHTGAGDQGGGQALMSPGSCLEDFRAAPFLECQGRQGTCHFFANKYSFWLTTVKADLQFSSAPAPDTLKESQAQRQKISRCQVCVKYS

Target ORF information:

RefSeq Version	XM_516128.5
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes collagen type IV alpha 4 chain (COL4A4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_516128.5

ORF Insert Sequence:

ATGTGGTCTC TGCACATAGT ACTAATGAGG TGCTCCTTCA GATTGACCAA GTCCTTGGCC 
ACAGATCCCT GGTCACTTAC ACTCATTCTC TTTTCTGTAC AATATGTATA TGGGAGTGGA 
AAGAAATACG TTGGTCCTTG TGGAGGAAGA GATTGCTCTG TTTGCCACTG TGTTCCTGAA 
AAGGGGTCTC GGGGTCCACC AGGACCACCA GGGCCACAGG GTCCAATTGG ACCCCTGGGA 
GCCCCAGGAC CCATTGGGCT TTCAGGAGAG AAAGGAATGA GAGGGGACCG CGGCCCTCCT 
GGAGCAGCAG GGGACAAAGG AGATAAGGGT TCAACTGGTG TTCCTGGATT TCCAGGTTTA 
GATGGCATAC CTGGGCACCC AGGGCCTCCT GGACCCAGAG GCAAACCTGG TATGAGTGGC 
CACAATGGCT CAAGAGGTGA CCCAGGGTTT CCAGGAGGAA GAGGAGCTCT TGGCCCAGGA 
GGCCCCCTAG GCCATCCTGG GGAAAAGGGA GAAAAAGGAA ATTCAGTGTT CATTTTAGGT 
GCCGTTAAAG GTATTCAGGG AGACAGAGGG GACCCAGGAC TGCCTGGCTT ACCAGGATCT 
TGGGGGGCAG GAGGACCGGC GGGTCCCACA GGATATCCTG GAGAGCCAGG GTTAGTGGGA 
CCTCCGGGCC AACCAGGGCG TCCAGGTTTG AAGGGAAATC CCGGTGTGGG AGTAAAGGGG 
CAAATGGGAG ACCCGGGTGA GGTTGGTCAG CAAGGTTCTC CTGGACCCAC CCTGTTGGTG 
GAGCCACCTG ACTTTCGTCT CTATAAAGGA GAAAAGGGTA TAAAAGGAAT TCCTGGAATG 
GTTGGACTGC CAGGACCACC AGGACGCAAG GGAGAATCTG ATATTGGGGC AAAAGGAGAA 
AAAGGTATTC CTGGATTTCC AGGGCCTCGG GGGGATCCTG GTTCCTATGG ATCTCCAGGT 
TTTCCAGGAT TAAAGGGAGA ACTAGGACTG GTTGGAGATC CTGGGCTATT TGGATTAATT 
GGCCCAAAGG GGGATCCTGG AAATCGAGGG CACCCAGGAC CACCAGGTGT TTTGGTGACT 
CCACCTCTTC CACTCAAAGG CCCACCAGGG GACCCAGGGT TCCCTGGCCG CTATGGAGAA 
ACAGGGGATG TTGGACCACC TGGTCCCCCA GGTCTCTTGG GCAGACCAGG GGAAGCCTGT 
GCAGGCATGA TAGGACCCCC TGGGCCACAA GGATTTCCTG GTCTTCCTGG GCTTCCAGGA 
GAAGCTGGTA TTCCTGGGAG ACCTGATTCT GCTCCAGGAA AACCAGGGAA GCCAGGATCA 
CCTGGCTTGC CTGGAGCACC AGGCCTGCAG GGCCTCCCAG GATCAAGTGT GATATACTGT 
AGTGTTGGGA ATCCTGGACC ACAAGGAATA AAAGGCAAAG TGGGTCCCCC AGGAGGAAGA 
GGCTCAAAAG GAGAAAAAGG AAATGAAGGA CTCTGTGCCT GTGAGCCTGG ACCCATGGGC 
CCCCCTGGCC CTCCAGGACT TCCTGGGAGG CAGGGGAGTA AGGGAGACTT GGGGCTCCCT 
GGCTGGCTTG GAACAAAAGG TGACCCAGGA CCTCCTGGTG CTGAAGGACC TCCAGGGCTA 
CCAGGAAAGC ATGGTGCCTC TGGACCACCT GGCAACAAAG GGGCGAAGGG TGACATGGTT 
GTATCAAGAG TTAAAGGGCA CAAAGGAGAA AGAGGTCCTG ATGGGCCCCC AGGATTTCCA 
GGGCAGCCAG GATCACATGG TCGGGATGGA CATGCTGGAG AAAAAGGGGA TCCAGGACCC 
CCAGGGGATC ATGAAGATGC GACCCCAGGT GGTAAAGGAT TTCCTGGACC TCTGGGCCCC 
CCGGGCAAAG CAGGACCTTT GGGGCCCCCA GGACTGGGAT TTCCTGGTCC ACCAGGACAG 
CGAGGCCACC CAGGAGTTCC AGGCCACCCA GGTGTGAGGG GCCCTGATGG CTTGAAGGGT 
CAGAAAGGTG ACACAATTTC TTGCAACGTA ACCTACCCTG GGAGGCACGG CCCTCCAGGT 
TTTGATGGAC CTCCAGGTCC GAAGGGATTT CCAGGTCCCC AAGGTGCCCC TGGGCTGAGT 
GGTTCAGATG GGCATAAAGG CAGACCTGGC ACACCAGGAA CAGCGGAAAT ACCAGGTCCA 
CCTGGTTTTC GTGGTGACAT GGGAGATCCG GGTTTTGGAG GTGAAAAGGG GTCCTCCCCT 
GCTGGGCCCC CAGGCCCTCC CGGCTCACCA GGAGTGAATG GTCAGAAAGG AATCCCGGGA 
GACCCTGCAT TTGGTCACCT GGGACCCCCG GGAAAGAGGG GTCTTTCAGG AGTGCCAGGG 
ATAAAAGGAC CCAGAGGTGA TCCGGGATGT CCAGGGGCTG AAGGGCCAGC TGGCATTCCT 
GGATTCCCAG GTCTCAAAGG TCCCAAAGGC AGAGAGGGAC ATGCTGGGTT TCCAGGTGTC 
CCAGGTCCAC CTGGCCATTC CTGTGAAAGA GGTGCTCCAG GGATACCAGG GCAACCGGGA 
CTCCCTGGGT ATCCAGGTAG CCCAGGTGCT CCAGGTGGGA AAGGACAGCT GGGAGATGTG 
GGGCCTCCCG GGCCAGCTGG AATGAAAGGC CTCCCTGGAC TCCCAGGATG GCCTGGGGCA 
CATGGTCCCC CAGGCCTCCC AGGAATCCCA GGTCCCTTTG GAGATGATGG GCTACCTGGT 
CCTCCAGGTC CAAAGGGACC CCAGGGGCTG CCTGGTTTCC CAGGTTTTCC CGGAGAAAGA 
GGAAAGCCTG GTGCAGAGGG ATGTCCTGGC ACAAAGGGAG AACCTGGAGA GAAGGGCATG 
TCTGGCCTTC CCGGAGACCG GGGACTGAGA GGGGCCAAAG GAGCCATAGG ACCTCCCGGA 
GATGAAGGAG AAATGGCTAT CATTTCCCAA AAGGGAACAC CTGGGGAACC TGGACCTCCT 
GGAGATGATG GATTCCCAGG AGAAAGAGGT GATAAAGGAA CTCCCGGGAT GCAAGGGAGA 
AGAGGAGAGC CGGGAAGATA CGGACCACCT GGATTTCAGA GAGGGGAACC TGGTGAGAAA 
GGTCAGCCAG GGCATCCTGG ACCCCCAGGC CCTCCAGGCT CAACTGGTCT AAGAGGGTTC 
ATTGGTTTTC CAGGACTTCC AGGTGACCAG GGTGAGCCAG GTTCTCCAGG TCCCCCTGGA 
TTTTCAGGAA TTGATGGAGC AAGAGGACCT AAAGGAAACA AAGGTGACCC TGCCAGTCAC 
TTTGGTCCAC CTGGTCCAAA GGGTGAGCCA GGTAGCCCTG GATGTCCAGG GCATTTTGGA 
GCATCTGGAG AGCAGGGCTT GCCTGGTATT CAAGGGCCCA GAGGATCACC TGGAAGGCCA 
GGGCCACCTG GCTCCTCTGG ACCACCAGGG TGCCCAGGTG ATCACGGGAT GCCTGGGCTG 
AGGGGACAGC CAGGAGAAAT GGGAGACCCT GGGCCAAGAG GCCTCCAGGG GGATCCAGGG 
ATACCAGGTC CTCCGGGAAT AAAAGGTCCC TCCGGATCAC CTGGCCTGAA CGGCTTGCAT 
GGATTGAAAG GTCAGAAAGG AACTAAAGGT GCTTCAGGTT TGCATGATGT GGGGCCACCT 
GGTCCAGTGG GAATACCTGG GCTAAAAGGG GAGAGAGGAG ACCCTGGGAG CCCAGGAATC 
TCTCCTCCAG GTCCTCGTGG AGAAAAAGGT CCCCCAGGAC CCCCAGGGAG TTCAGGACCA 
CCTGGTCCTG CAGGTGCCAC AGGAAGAGCT CCTAAGGACA TTCCTGACCC GGGTCCACCT 
GGAGATCAGG GACCTCCTGG TCCTGATGGC CCAAGAGGAG CACCTGGGCC TCCAGGCCTC 
CCTGGGAGTG TTGACCTTCT GAGAGGGGAG CCAGGTGACT GTGGTCTACC AGGGCCACCA 
GGTCCCCCTG GCCCACCAGG CCCTCCAGGA TACAAAGGCT TTCCAGGATG TGATGGAAAA 
GATGGCCAGA AAGGACCAAT GGGATTCCCG GGACCGCAGG GACCACATGG ATTTCCTGGG 
CCACCTGGAG AGAAGGGTTT ACCTGGACCT CCAGGGAGAA AAGGGCCCAC TGGTCTTCCA 
GGTCCCAGAG GTGAACCGGG GCCACCTGCA GATGTGGATG ACTGTCCCCG AATCCCAGGC 
CTTCCTGGGG CGCCAGGCAT GAGAGGACCA GAAGGAGCCA TGGGGCTCTC TGGAATGAGA 
GGCCCCCCAG GACCAGGGTG CAAAGGAGAG CCTGGGCTGG ATGGCAGGAG GGGTGTGGAT 
GGCGTCCCTG GGTCTCCTGG GCCTCCCGGA CGTAAAGGTG ACACAGGAGA AGACGGCTAC 
CCTGGAGGAC CAGGGCCTCC TGGTCCCATT GGGGATCCTG GGCCCAAAGG GTTTGGCCCT 
GGATACCTCG GTGGCTTCCT CCTGGTTCTC CACAGTCAGA CGGACCAGGA GCCCACCTGC 
CCCCTGGGCA TGCCCAGGCT CTGGACTGGG TATAGTCTGT TATACCTGGA AGGGCAAGAG 
AAAGCTCACA ATCAAGACCT TGGTCTGGCA GGGTCTTGCC TTCCCGTGTT TAGCACGCTG 
CCCTTTGCCT ACTGCAACAT CCACCAGGTG TGCCACTATG CCCAGAGAAA CGACAGATCC 
TACTGGCTGG CCAGCGCTGC GCCCCTCCCC ATGATGCCAC TCTCTGAAGA GGCGATCCGC 
CCCTATGTCA GCCGCTGTGC GGTATGCGAG GCCCCGGCCC AGGCGGTGGC GGTGCACAGC 
CAGGACCAGT CCATCCCCCC ATGTCCGCAG ACCTGGAGGA GCCTCTGGAT CGGGTATTCA 
TTCCTGATGC ACACAGGAGC TGGGGACCAA GGAGGAGGGC AGGCCCTTAT GTCACCTGGC 
AGCTGCCTGG AAGATTTCAG AGCAGCACCA TTCCTTGAAT GCCAGGGCCG GCAGGGAACT 
TGCCACTTTT TTGCAAATAA GTATAGCTTC TGGCTCACAA CGGTGAAAGC AGACTTGCAG 
TTTTCCTCTG CTCCAGCACC AGACACCTTA AAAGAAAGCC AGGCCCAACG CCAGAAAATC 
AGCCGGTGCC AGGTCTGCGT GAAGTATAGC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL4A4 ( XM_009444464.3 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_009442739.3 Pan troglodytes collagen type IV alpha 4 chain (COL4A4), transcript variant X2, mRNA.

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CloneID	OPc104269
Clone ID Related Accession (Same CDS sequence)	XM_009444465.3 , XM_516128.5 , XM_009444464.3 , XM_016950591.2
Accession Version	XM_009444464.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5073bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	collagen alpha-4(IV) chain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036881.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_009444464.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGTCTC TGCACATAGT ACTAATGAGG TGCTCCTTCA GATTGACCAA GTCCTTGGCC 
ACAGATCCCT GGTCACTTAC ACTCATTCTC TTTTCTGTAC AATATGTATA TGGGAGTGGA 
AAGAAATACG TTGGTCCTTG TGGAGGAAGA GATTGCTCTG TTTGCCACTG TGTTCCTGAA 
AAGGGGTCTC GGGGTCCACC AGGACCACCA GGGCCACAGG GTCCAATTGG ACCCCTGGGA 
GCCCCAGGAC CCATTGGGCT TTCAGGAGAG AAAGGAATGA GAGGGGACCG CGGCCCTCCT 
GGAGCAGCAG GGGACAAAGG AGATAAGGGT TCAACTGGTG TTCCTGGATT TCCAGGTTTA 
GATGGCATAC CTGGGCACCC AGGGCCTCCT GGACCCAGAG GCAAACCTGG TATGAGTGGC 
CACAATGGCT CAAGAGGTGA CCCAGGGTTT CCAGGAGGAA GAGGAGCTCT TGGCCCAGGA 
GGCCCCCTAG GCCATCCTGG GGAAAAGGGA GAAAAAGGAA ATTCAGTGTT CATTTTAGGT 
GCCGTTAAAG GTATTCAGGG AGACAGAGGG GACCCAGGAC TGCCTGGCTT ACCAGGATCT 
TGGGGGGCAG GAGGACCGGC GGGTCCCACA GGATATCCTG GAGAGCCAGG GTTAGTGGGA 
CCTCCGGGCC AACCAGGGCG TCCAGGTTTG AAGGGAAATC CCGGTGTGGG AGTAAAGGGG 
CAAATGGGAG ACCCGGGTGA GGTTGGTCAG CAAGGTTCTC CTGGACCCAC CCTGTTGGTG 
GAGCCACCTG ACTTTCGTCT CTATAAAGGA GAAAAGGGTA TAAAAGGAAT TCCTGGAATG 
GTTGGACTGC CAGGACCACC AGGACGCAAG GGAGAATCTG ATATTGGGGC AAAAGGAGAA 
AAAGGTATTC CTGGATTTCC AGGGCCTCGG GGGGATCCTG GTTCCTATGG ATCTCCAGGT 
TTTCCAGGAT TAAAGGGAGA ACTAGGACTG GTTGGAGATC CTGGGCTATT TGGATTAATT 
GGCCCAAAGG GGGATCCTGG AAATCGAGGG CACCCAGGAC CACCAGGTGT TTTGGTGACT 
CCACCTCTTC CACTCAAAGG CCCACCAGGG GACCCAGGGT TCCCTGGCCG CTATGGAGAA 
ACAGGGGATG TTGGACCACC TGGTCCCCCA GGTCTCTTGG GCAGACCAGG GGAAGCCTGT 
GCAGGCATGA TAGGACCCCC TGGGCCACAA GGATTTCCTG GTCTTCCTGG GCTTCCAGGA 
GAAGCTGGTA TTCCTGGGAG ACCTGATTCT GCTCCAGGAA AACCAGGGAA GCCAGGATCA 
CCTGGCTTGC CTGGAGCACC AGGCCTGCAG GGCCTCCCAG GATCAAGTGT GATATACTGT 
AGTGTTGGGA ATCCTGGACC ACAAGGAATA AAAGGCAAAG TGGGTCCCCC AGGAGGAAGA 
GGCTCAAAAG GAGAAAAAGG AAATGAAGGA CTCTGTGCCT GTGAGCCTGG ACCCATGGGC 
CCCCCTGGCC CTCCAGGACT TCCTGGGAGG CAGGGGAGTA AGGGAGACTT GGGGCTCCCT 
GGCTGGCTTG GAACAAAAGG TGACCCAGGA CCTCCTGGTG CTGAAGGACC TCCAGGGCTA 
CCAGGAAAGC ATGGTGCCTC TGGACCACCT GGCAACAAAG GGGCGAAGGG TGACATGGTT 
GTATCAAGAG TTAAAGGGCA CAAAGGAGAA AGAGGTCCTG ATGGGCCCCC AGGATTTCCA 
GGGCAGCCAG GATCACATGG TCGGGATGGA CATGCTGGAG AAAAAGGGGA TCCAGGACCC 
CCAGGGGATC ATGAAGATGC GACCCCAGGT GGTAAAGGAT TTCCTGGACC TCTGGGCCCC 
CCGGGCAAAG CAGGACCTTT GGGGCCCCCA GGACTGGGAT TTCCTGGTCC ACCAGGACAG 
CGAGGCCACC CAGGAGTTCC AGGCCACCCA GGTGTGAGGG GCCCTGATGG CTTGAAGGGT 
CAGAAAGGTG ACACAATTTC TTGCAACGTA ACCTACCCTG GGAGGCACGG CCCTCCAGGT 
TTTGATGGAC CTCCAGGTCC GAAGGGATTT CCAGGTCCCC AAGGTGCCCC TGGGCTGAGT 
GGTTCAGATG GGCATAAAGG CAGACCTGGC ACACCAGGAA CAGCGGAAAT ACCAGGTCCA 
CCTGGTTTTC GTGGTGACAT GGGAGATCCG GGTTTTGGAG GTGAAAAGGG GTCCTCCCCT 
GCTGGGCCCC CAGGCCCTCC CGGCTCACCA GGAGTGAATG GTCAGAAAGG AATCCCGGGA 
GACCCTGCAT TTGGTCACCT GGGACCCCCG GGAAAGAGGG GTCTTTCAGG AGTGCCAGGG 
ATAAAAGGAC CCAGAGGTGA TCCGGGATGT CCAGGGGCTG AAGGGCCAGC TGGCATTCCT 
GGATTCCCAG GTCTCAAAGG TCCCAAAGGC AGAGAGGGAC ATGCTGGGTT TCCAGGTGTC 
CCAGGTCCAC CTGGCCATTC CTGTGAAAGA GGTGCTCCAG GGATACCAGG GCAACCGGGA 
CTCCCTGGGT ATCCAGGTAG CCCAGGTGCT CCAGGTGGGA AAGGACAGCT GGGAGATGTG 
GGGCCTCCCG GGCCAGCTGG AATGAAAGGC CTCCCTGGAC TCCCAGGATG GCCTGGGGCA 
CATGGTCCCC CAGGCCTCCC AGGAATCCCA GGTCCCTTTG GAGATGATGG GCTACCTGGT 
CCTCCAGGTC CAAAGGGACC CCAGGGGCTG CCTGGTTTCC CAGGTTTTCC CGGAGAAAGA 
GGAAAGCCTG GTGCAGAGGG ATGTCCTGGC ACAAAGGGAG AACCTGGAGA GAAGGGCATG 
TCTGGCCTTC CCGGAGACCG GGGACTGAGA GGGGCCAAAG GAGCCATAGG ACCTCCCGGA 
GATGAAGGAG AAATGGCTAT CATTTCCCAA AAGGGAACAC CTGGGGAACC TGGACCTCCT 
GGAGATGATG GATTCCCAGG AGAAAGAGGT GATAAAGGAA CTCCCGGGAT GCAAGGGAGA 
AGAGGAGAGC CGGGAAGATA CGGACCACCT GGATTTCAGA GAGGGGAACC TGGTGAGAAA 
GGTCAGCCAG GGCATCCTGG ACCCCCAGGC CCTCCAGGCT CAACTGGTCT AAGAGGGTTC 
ATTGGTTTTC CAGGACTTCC AGGTGACCAG GGTGAGCCAG GTTCTCCAGG TCCCCCTGGA 
TTTTCAGGAA TTGATGGAGC AAGAGGACCT AAAGGAAACA AAGGTGACCC TGCCAGTCAC 
TTTGGTCCAC CTGGTCCAAA GGGTGAGCCA GGTAGCCCTG GATGTCCAGG GCATTTTGGA 
GCATCTGGAG AGCAGGGCTT GCCTGGTATT CAAGGGCCCA GAGGATCACC TGGAAGGCCA 
GGGCCACCTG GCTCCTCTGG ACCACCAGGG TGCCCAGGTG ATCACGGGAT GCCTGGGCTG 
AGGGGACAGC CAGGAGAAAT GGGAGACCCT GGGCCAAGAG GCCTCCAGGG GGATCCAGGG 
ATACCAGGTC CTCCGGGAAT AAAAGGTCCC TCCGGATCAC CTGGCCTGAA CGGCTTGCAT 
GGATTGAAAG GTCAGAAAGG AACTAAAGGT GCTTCAGGTT TGCATGATGT GGGGCCACCT 
GGTCCAGTGG GAATACCTGG GCTAAAAGGG GAGAGAGGAG ACCCTGGGAG CCCAGGAATC 
TCTCCTCCAG GTCCTCGTGG AGAAAAAGGT CCCCCAGGAC CCCCAGGGAG TTCAGGACCA 
CCTGGTCCTG CAGGTGCCAC AGGAAGAGCT CCTAAGGACA TTCCTGACCC GGGTCCACCT 
GGAGATCAGG GACCTCCTGG TCCTGATGGC CCAAGAGGAG CACCTGGGCC TCCAGGCCTC 
CCTGGGAGTG TTGACCTTCT GAGAGGGGAG CCAGGTGACT GTGGTCTACC AGGGCCACCA 
GGTCCCCCTG GCCCACCAGG CCCTCCAGGA TACAAAGGCT TTCCAGGATG TGATGGAAAA 
GATGGCCAGA AAGGACCAAT GGGATTCCCG GGACCGCAGG GACCACATGG ATTTCCTGGG 
CCACCTGGAG AGAAGGGTTT ACCTGGACCT CCAGGGAGAA AAGGGCCCAC TGGTCTTCCA 
GGTCCCAGAG GTGAACCGGG GCCACCTGCA GATGTGGATG ACTGTCCCCG AATCCCAGGC 
CTTCCTGGGG CGCCAGGCAT GAGAGGACCA GAAGGAGCCA TGGGGCTCTC TGGAATGAGA 
GGCCCCCCAG GACCAGGGTG CAAAGGAGAG CCTGGGCTGG ATGGCAGGAG GGGTGTGGAT 
GGCGTCCCTG GGTCTCCTGG GCCTCCCGGA CGTAAAGGTG ACACAGGAGA AGACGGCTAC 
CCTGGAGGAC CAGGGCCTCC TGGTCCCATT GGGGATCCTG GGCCCAAAGG GTTTGGCCCT 
GGATACCTCG GTGGCTTCCT CCTGGTTCTC CACAGTCAGA CGGACCAGGA GCCCACCTGC 
CCCCTGGGCA TGCCCAGGCT CTGGACTGGG TATAGTCTGT TATACCTGGA AGGGCAAGAG 
AAAGCTCACA ATCAAGACCT TGGTCTGGCA GGGTCTTGCC TTCCCGTGTT TAGCACGCTG 
CCCTTTGCCT ACTGCAACAT CCACCAGGTG TGCCACTATG CCCAGAGAAA CGACAGATCC 
TACTGGCTGG CCAGCGCTGC GCCCCTCCCC ATGATGCCAC TCTCTGAAGA GGCGATCCGC 
CCCTATGTCA GCCGCTGTGC GGTATGCGAG GCCCCGGCCC AGGCGGTGGC GGTGCACAGC 
CAGGACCAGT CCATCCCCCC ATGTCCGCAG ACCTGGAGGA GCCTCTGGAT CGGGTATTCA 
TTCCTGATGC ACACAGGAGC TGGGGACCAA GGAGGAGGGC AGGCCCTTAT GTCACCTGGC 
AGCTGCCTGG AAGATTTCAG AGCAGCACCA TTCCTTGAAT GCCAGGGCCG GCAGGGAACT 
TGCCACTTTT TTGCAAATAA GTATAGCTTC TGGCTCACAA CGGTGAAAGC AGACTTGCAG 
TTTTCCTCTG CTCCAGCACC AGACACCTTA AAAGAAAGCC AGGCCCAACG CCAGAAAATC 
AGCCGGTGCC AGGTCTGCGT GAAGTATAGC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009442739.3
CDS	190..5262
Translation	MWSLHIVLMRCSFRLTKSLATDPWSLTLILFSVQYVYGSGKKYVGPCGGRDCSVCHCVPEKGSRGPPGPPGPQGPIGPLGAPGPIGLSGEKGMRGDRGPPGAAGDKGDKGSTGVPGFPGLDGIPGHPGPPGPRGKPGMSGHNGSRGDPGFPGGRGALGPGGPLGHPGEKGEKGNSVFILGAVKGIQGDRGDPGLPGLPGSWGAGGPAGPTGYPGEPGLVGPPGQPGRPGLKGNPGVGVKGQMGDPGEVGQQGSPGPTLLVEPPDFRLYKGEKGIKGIPGMVGLPGPPGRKGESDIGAKGEKGIPGFPGPRGDPGSYGSPGFPGLKGELGLVGDPGLFGLIGPKGDPGNRGHPGPPGVLVTPPLPLKGPPGDPGFPGRYGETGDVGPPGPPGLLGRPGEACAGMIGPPGPQGFPGLPGLPGEAGIPGRPDSAPGKPGKPGSPGLPGAPGLQGLPGSSVIYCSVGNPGPQGIKGKVGPPGGRGSKGEKGNEGLCACEPGPMGPPGPPGLPGRQGSKGDLGLPGWLGTKGDPGPPGAEGPPGLPGKHGASGPPGNKGAKGDMVVSRVKGHKGERGPDGPPGFPGQPGSHGRDGHAGEKGDPGPPGDHEDATPGGKGFPGPLGPPGKAGPLGPPGLGFPGPPGQRGHPGVPGHPGVRGPDGLKGQKGDTISCNVTYPGRHGPPGFDGPPGPKGFPGPQGAPGLSGSDGHKGRPGTPGTAEIPGPPGFRGDMGDPGFGGEKGSSPAGPPGPPGSPGVNGQKGIPGDPAFGHLGPPGKRGLSGVPGIKGPRGDPGCPGAEGPAGIPGFPGLKGPKGREGHAGFPGVPGPPGHSCERGAPGIPGQPGLPGYPGSPGAPGGKGQLGDVGPPGPAGMKGLPGLPGWPGAHGPPGLPGIPGPFGDDGLPGPPGPKGPQGLPGFPGFPGERGKPGAEGCPGTKGEPGEKGMSGLPGDRGLRGAKGAIGPPGDEGEMAIISQKGTPGEPGPPGDDGFPGERGDKGTPGMQGRRGEPGRYGPPGFQRGEPGEKGQPGHPGPPGPPGSTGLRGFIGFPGLPGDQGEPGSPGPPGFSGIDGARGPKGNKGDPASHFGPPGPKGEPGSPGCPGHFGASGEQGLPGIQGPRGSPGRPGPPGSSGPPGCPGDHGMPGLRGQPGEMGDPGPRGLQGDPGIPGPPGIKGPSGSPGLNGLHGLKGQKGTKGASGLHDVGPPGPVGIPGLKGERGDPGSPGISPPGPRGEKGPPGPPGSSGPPGPAGATGRAPKDIPDPGPPGDQGPPGPDGPRGAPGPPGLPGSVDLLRGEPGDCGLPGPPGPPGPPGPPGYKGFPGCDGKDGQKGPMGFPGPQGPHGFPGPPGEKGLPGPPGRKGPTGLPGPRGEPGPPADVDDCPRIPGLPGAPGMRGPEGAMGLSGMRGPPGPGCKGEPGLDGRRGVDGVPGSPGPPGRKGDTGEDGYPGGPGPPGPIGDPGPKGFGPGYLGGFLLVLHSQTDQEPTCPLGMPRLWTGYSLLYLEGQEKAHNQDLGLAGSCLPVFSTLPFAYCNIHQVCHYAQRNDRSYWLASAAPLPMMPLSEEAIRPYVSRCAVCEAPAQAVAVHSQDQSIPPCPQTWRSLWIGYSFLMHTGAGDQGGGQALMSPGSCLEDFRAAPFLECQGRQGTCHFFANKYSFWLTTVKADLQFSSAPAPDTLKESQAQRQKISRCQVCVKYS

Target ORF information:

RefSeq Version	XM_009444464.3
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes collagen type IV alpha 4 chain (COL4A4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009444464.3

ORF Insert Sequence:

ATGTGGTCTC TGCACATAGT ACTAATGAGG TGCTCCTTCA GATTGACCAA GTCCTTGGCC 
ACAGATCCCT GGTCACTTAC ACTCATTCTC TTTTCTGTAC AATATGTATA TGGGAGTGGA 
AAGAAATACG TTGGTCCTTG TGGAGGAAGA GATTGCTCTG TTTGCCACTG TGTTCCTGAA 
AAGGGGTCTC GGGGTCCACC AGGACCACCA GGGCCACAGG GTCCAATTGG ACCCCTGGGA 
GCCCCAGGAC CCATTGGGCT TTCAGGAGAG AAAGGAATGA GAGGGGACCG CGGCCCTCCT 
GGAGCAGCAG GGGACAAAGG AGATAAGGGT TCAACTGGTG TTCCTGGATT TCCAGGTTTA 
GATGGCATAC CTGGGCACCC AGGGCCTCCT GGACCCAGAG GCAAACCTGG TATGAGTGGC 
CACAATGGCT CAAGAGGTGA CCCAGGGTTT CCAGGAGGAA GAGGAGCTCT TGGCCCAGGA 
GGCCCCCTAG GCCATCCTGG GGAAAAGGGA GAAAAAGGAA ATTCAGTGTT CATTTTAGGT 
GCCGTTAAAG GTATTCAGGG AGACAGAGGG GACCCAGGAC TGCCTGGCTT ACCAGGATCT 
TGGGGGGCAG GAGGACCGGC GGGTCCCACA GGATATCCTG GAGAGCCAGG GTTAGTGGGA 
CCTCCGGGCC AACCAGGGCG TCCAGGTTTG AAGGGAAATC CCGGTGTGGG AGTAAAGGGG 
CAAATGGGAG ACCCGGGTGA GGTTGGTCAG CAAGGTTCTC CTGGACCCAC CCTGTTGGTG 
GAGCCACCTG ACTTTCGTCT CTATAAAGGA GAAAAGGGTA TAAAAGGAAT TCCTGGAATG 
GTTGGACTGC CAGGACCACC AGGACGCAAG GGAGAATCTG ATATTGGGGC AAAAGGAGAA 
AAAGGTATTC CTGGATTTCC AGGGCCTCGG GGGGATCCTG GTTCCTATGG ATCTCCAGGT 
TTTCCAGGAT TAAAGGGAGA ACTAGGACTG GTTGGAGATC CTGGGCTATT TGGATTAATT 
GGCCCAAAGG GGGATCCTGG AAATCGAGGG CACCCAGGAC CACCAGGTGT TTTGGTGACT 
CCACCTCTTC CACTCAAAGG CCCACCAGGG GACCCAGGGT TCCCTGGCCG CTATGGAGAA 
ACAGGGGATG TTGGACCACC TGGTCCCCCA GGTCTCTTGG GCAGACCAGG GGAAGCCTGT 
GCAGGCATGA TAGGACCCCC TGGGCCACAA GGATTTCCTG GTCTTCCTGG GCTTCCAGGA 
GAAGCTGGTA TTCCTGGGAG ACCTGATTCT GCTCCAGGAA AACCAGGGAA GCCAGGATCA 
CCTGGCTTGC CTGGAGCACC AGGCCTGCAG GGCCTCCCAG GATCAAGTGT GATATACTGT 
AGTGTTGGGA ATCCTGGACC ACAAGGAATA AAAGGCAAAG TGGGTCCCCC AGGAGGAAGA 
GGCTCAAAAG GAGAAAAAGG AAATGAAGGA CTCTGTGCCT GTGAGCCTGG ACCCATGGGC 
CCCCCTGGCC CTCCAGGACT TCCTGGGAGG CAGGGGAGTA AGGGAGACTT GGGGCTCCCT 
GGCTGGCTTG GAACAAAAGG TGACCCAGGA CCTCCTGGTG CTGAAGGACC TCCAGGGCTA 
CCAGGAAAGC ATGGTGCCTC TGGACCACCT GGCAACAAAG GGGCGAAGGG TGACATGGTT 
GTATCAAGAG TTAAAGGGCA CAAAGGAGAA AGAGGTCCTG ATGGGCCCCC AGGATTTCCA 
GGGCAGCCAG GATCACATGG TCGGGATGGA CATGCTGGAG AAAAAGGGGA TCCAGGACCC 
CCAGGGGATC ATGAAGATGC GACCCCAGGT GGTAAAGGAT TTCCTGGACC TCTGGGCCCC 
CCGGGCAAAG CAGGACCTTT GGGGCCCCCA GGACTGGGAT TTCCTGGTCC ACCAGGACAG 
CGAGGCCACC CAGGAGTTCC AGGCCACCCA GGTGTGAGGG GCCCTGATGG CTTGAAGGGT 
CAGAAAGGTG ACACAATTTC TTGCAACGTA ACCTACCCTG GGAGGCACGG CCCTCCAGGT 
TTTGATGGAC CTCCAGGTCC GAAGGGATTT CCAGGTCCCC AAGGTGCCCC TGGGCTGAGT 
GGTTCAGATG GGCATAAAGG CAGACCTGGC ACACCAGGAA CAGCGGAAAT ACCAGGTCCA 
CCTGGTTTTC GTGGTGACAT GGGAGATCCG GGTTTTGGAG GTGAAAAGGG GTCCTCCCCT 
GCTGGGCCCC CAGGCCCTCC CGGCTCACCA GGAGTGAATG GTCAGAAAGG AATCCCGGGA 
GACCCTGCAT TTGGTCACCT GGGACCCCCG GGAAAGAGGG GTCTTTCAGG AGTGCCAGGG 
ATAAAAGGAC CCAGAGGTGA TCCGGGATGT CCAGGGGCTG AAGGGCCAGC TGGCATTCCT 
GGATTCCCAG GTCTCAAAGG TCCCAAAGGC AGAGAGGGAC ATGCTGGGTT TCCAGGTGTC 
CCAGGTCCAC CTGGCCATTC CTGTGAAAGA GGTGCTCCAG GGATACCAGG GCAACCGGGA 
CTCCCTGGGT ATCCAGGTAG CCCAGGTGCT CCAGGTGGGA AAGGACAGCT GGGAGATGTG 
GGGCCTCCCG GGCCAGCTGG AATGAAAGGC CTCCCTGGAC TCCCAGGATG GCCTGGGGCA 
CATGGTCCCC CAGGCCTCCC AGGAATCCCA GGTCCCTTTG GAGATGATGG GCTACCTGGT 
CCTCCAGGTC CAAAGGGACC CCAGGGGCTG CCTGGTTTCC CAGGTTTTCC CGGAGAAAGA 
GGAAAGCCTG GTGCAGAGGG ATGTCCTGGC ACAAAGGGAG AACCTGGAGA GAAGGGCATG 
TCTGGCCTTC CCGGAGACCG GGGACTGAGA GGGGCCAAAG GAGCCATAGG ACCTCCCGGA 
GATGAAGGAG AAATGGCTAT CATTTCCCAA AAGGGAACAC CTGGGGAACC TGGACCTCCT 
GGAGATGATG GATTCCCAGG AGAAAGAGGT GATAAAGGAA CTCCCGGGAT GCAAGGGAGA 
AGAGGAGAGC CGGGAAGATA CGGACCACCT GGATTTCAGA GAGGGGAACC TGGTGAGAAA 
GGTCAGCCAG GGCATCCTGG ACCCCCAGGC CCTCCAGGCT CAACTGGTCT AAGAGGGTTC 
ATTGGTTTTC CAGGACTTCC AGGTGACCAG GGTGAGCCAG GTTCTCCAGG TCCCCCTGGA 
TTTTCAGGAA TTGATGGAGC AAGAGGACCT AAAGGAAACA AAGGTGACCC TGCCAGTCAC 
TTTGGTCCAC CTGGTCCAAA GGGTGAGCCA GGTAGCCCTG GATGTCCAGG GCATTTTGGA 
GCATCTGGAG AGCAGGGCTT GCCTGGTATT CAAGGGCCCA GAGGATCACC TGGAAGGCCA 
GGGCCACCTG GCTCCTCTGG ACCACCAGGG TGCCCAGGTG ATCACGGGAT GCCTGGGCTG 
AGGGGACAGC CAGGAGAAAT GGGAGACCCT GGGCCAAGAG GCCTCCAGGG GGATCCAGGG 
ATACCAGGTC CTCCGGGAAT AAAAGGTCCC TCCGGATCAC CTGGCCTGAA CGGCTTGCAT 
GGATTGAAAG GTCAGAAAGG AACTAAAGGT GCTTCAGGTT TGCATGATGT GGGGCCACCT 
GGTCCAGTGG GAATACCTGG GCTAAAAGGG GAGAGAGGAG ACCCTGGGAG CCCAGGAATC 
TCTCCTCCAG GTCCTCGTGG AGAAAAAGGT CCCCCAGGAC CCCCAGGGAG TTCAGGACCA 
CCTGGTCCTG CAGGTGCCAC AGGAAGAGCT CCTAAGGACA TTCCTGACCC GGGTCCACCT 
GGAGATCAGG GACCTCCTGG TCCTGATGGC CCAAGAGGAG CACCTGGGCC TCCAGGCCTC 
CCTGGGAGTG TTGACCTTCT GAGAGGGGAG CCAGGTGACT GTGGTCTACC AGGGCCACCA 
GGTCCCCCTG GCCCACCAGG CCCTCCAGGA TACAAAGGCT TTCCAGGATG TGATGGAAAA 
GATGGCCAGA AAGGACCAAT GGGATTCCCG GGACCGCAGG GACCACATGG ATTTCCTGGG 
CCACCTGGAG AGAAGGGTTT ACCTGGACCT CCAGGGAGAA AAGGGCCCAC TGGTCTTCCA 
GGTCCCAGAG GTGAACCGGG GCCACCTGCA GATGTGGATG ACTGTCCCCG AATCCCAGGC 
CTTCCTGGGG CGCCAGGCAT GAGAGGACCA GAAGGAGCCA TGGGGCTCTC TGGAATGAGA 
GGCCCCCCAG GACCAGGGTG CAAAGGAGAG CCTGGGCTGG ATGGCAGGAG GGGTGTGGAT 
GGCGTCCCTG GGTCTCCTGG GCCTCCCGGA CGTAAAGGTG ACACAGGAGA AGACGGCTAC 
CCTGGAGGAC CAGGGCCTCC TGGTCCCATT GGGGATCCTG GGCCCAAAGG GTTTGGCCCT 
GGATACCTCG GTGGCTTCCT CCTGGTTCTC CACAGTCAGA CGGACCAGGA GCCCACCTGC 
CCCCTGGGCA TGCCCAGGCT CTGGACTGGG TATAGTCTGT TATACCTGGA AGGGCAAGAG 
AAAGCTCACA ATCAAGACCT TGGTCTGGCA GGGTCTTGCC TTCCCGTGTT TAGCACGCTG 
CCCTTTGCCT ACTGCAACAT CCACCAGGTG TGCCACTATG CCCAGAGAAA CGACAGATCC 
TACTGGCTGG CCAGCGCTGC GCCCCTCCCC ATGATGCCAC TCTCTGAAGA GGCGATCCGC 
CCCTATGTCA GCCGCTGTGC GGTATGCGAG GCCCCGGCCC AGGCGGTGGC GGTGCACAGC 
CAGGACCAGT CCATCCCCCC ATGTCCGCAG ACCTGGAGGA GCCTCTGGAT CGGGTATTCA 
TTCCTGATGC ACACAGGAGC TGGGGACCAA GGAGGAGGGC AGGCCCTTAT GTCACCTGGC 
AGCTGCCTGG AAGATTTCAG AGCAGCACCA TTCCTTGAAT GCCAGGGCCG GCAGGGAACT 
TGCCACTTTT TTGCAAATAA GTATAGCTTC TGGCTCACAA CGGTGAAAGC AGACTTGCAG 
TTTTCCTCTG CTCCAGCACC AGACACCTTA AAAGAAAGCC AGGCCCAACG CCAGAAAATC 
AGCCGGTGCC AGGTCTGCGT GAAGTATAGC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL4A4 ( XM_016950591.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016806080.2 Pan troglodytes collagen type IV alpha 4 chain (COL4A4), transcript variant X3, mRNA.

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CloneID	OPc104269
Clone ID Related Accession (Same CDS sequence)	XM_009444465.3 , XM_516128.5 , XM_009444464.3 , XM_016950591.2
Accession Version	XM_016950591.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5073bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	collagen alpha-4(IV) chain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036881.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_016950591.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGTCTC TGCACATAGT ACTAATGAGG TGCTCCTTCA GATTGACCAA GTCCTTGGCC 
ACAGATCCCT GGTCACTTAC ACTCATTCTC TTTTCTGTAC AATATGTATA TGGGAGTGGA 
AAGAAATACG TTGGTCCTTG TGGAGGAAGA GATTGCTCTG TTTGCCACTG TGTTCCTGAA 
AAGGGGTCTC GGGGTCCACC AGGACCACCA GGGCCACAGG GTCCAATTGG ACCCCTGGGA 
GCCCCAGGAC CCATTGGGCT TTCAGGAGAG AAAGGAATGA GAGGGGACCG CGGCCCTCCT 
GGAGCAGCAG GGGACAAAGG AGATAAGGGT TCAACTGGTG TTCCTGGATT TCCAGGTTTA 
GATGGCATAC CTGGGCACCC AGGGCCTCCT GGACCCAGAG GCAAACCTGG TATGAGTGGC 
CACAATGGCT CAAGAGGTGA CCCAGGGTTT CCAGGAGGAA GAGGAGCTCT TGGCCCAGGA 
GGCCCCCTAG GCCATCCTGG GGAAAAGGGA GAAAAAGGAA ATTCAGTGTT CATTTTAGGT 
GCCGTTAAAG GTATTCAGGG AGACAGAGGG GACCCAGGAC TGCCTGGCTT ACCAGGATCT 
TGGGGGGCAG GAGGACCGGC GGGTCCCACA GGATATCCTG GAGAGCCAGG GTTAGTGGGA 
CCTCCGGGCC AACCAGGGCG TCCAGGTTTG AAGGGAAATC CCGGTGTGGG AGTAAAGGGG 
CAAATGGGAG ACCCGGGTGA GGTTGGTCAG CAAGGTTCTC CTGGACCCAC CCTGTTGGTG 
GAGCCACCTG ACTTTCGTCT CTATAAAGGA GAAAAGGGTA TAAAAGGAAT TCCTGGAATG 
GTTGGACTGC CAGGACCACC AGGACGCAAG GGAGAATCTG ATATTGGGGC AAAAGGAGAA 
AAAGGTATTC CTGGATTTCC AGGGCCTCGG GGGGATCCTG GTTCCTATGG ATCTCCAGGT 
TTTCCAGGAT TAAAGGGAGA ACTAGGACTG GTTGGAGATC CTGGGCTATT TGGATTAATT 
GGCCCAAAGG GGGATCCTGG AAATCGAGGG CACCCAGGAC CACCAGGTGT TTTGGTGACT 
CCACCTCTTC CACTCAAAGG CCCACCAGGG GACCCAGGGT TCCCTGGCCG CTATGGAGAA 
ACAGGGGATG TTGGACCACC TGGTCCCCCA GGTCTCTTGG GCAGACCAGG GGAAGCCTGT 
GCAGGCATGA TAGGACCCCC TGGGCCACAA GGATTTCCTG GTCTTCCTGG GCTTCCAGGA 
GAAGCTGGTA TTCCTGGGAG ACCTGATTCT GCTCCAGGAA AACCAGGGAA GCCAGGATCA 
CCTGGCTTGC CTGGAGCACC AGGCCTGCAG GGCCTCCCAG GATCAAGTGT GATATACTGT 
AGTGTTGGGA ATCCTGGACC ACAAGGAATA AAAGGCAAAG TGGGTCCCCC AGGAGGAAGA 
GGCTCAAAAG GAGAAAAAGG AAATGAAGGA CTCTGTGCCT GTGAGCCTGG ACCCATGGGC 
CCCCCTGGCC CTCCAGGACT TCCTGGGAGG CAGGGGAGTA AGGGAGACTT GGGGCTCCCT 
GGCTGGCTTG GAACAAAAGG TGACCCAGGA CCTCCTGGTG CTGAAGGACC TCCAGGGCTA 
CCAGGAAAGC ATGGTGCCTC TGGACCACCT GGCAACAAAG GGGCGAAGGG TGACATGGTT 
GTATCAAGAG TTAAAGGGCA CAAAGGAGAA AGAGGTCCTG ATGGGCCCCC AGGATTTCCA 
GGGCAGCCAG GATCACATGG TCGGGATGGA CATGCTGGAG AAAAAGGGGA TCCAGGACCC 
CCAGGGGATC ATGAAGATGC GACCCCAGGT GGTAAAGGAT TTCCTGGACC TCTGGGCCCC 
CCGGGCAAAG CAGGACCTTT GGGGCCCCCA GGACTGGGAT TTCCTGGTCC ACCAGGACAG 
CGAGGCCACC CAGGAGTTCC AGGCCACCCA GGTGTGAGGG GCCCTGATGG CTTGAAGGGT 
CAGAAAGGTG ACACAATTTC TTGCAACGTA ACCTACCCTG GGAGGCACGG CCCTCCAGGT 
TTTGATGGAC CTCCAGGTCC GAAGGGATTT CCAGGTCCCC AAGGTGCCCC TGGGCTGAGT 
GGTTCAGATG GGCATAAAGG CAGACCTGGC ACACCAGGAA CAGCGGAAAT ACCAGGTCCA 
CCTGGTTTTC GTGGTGACAT GGGAGATCCG GGTTTTGGAG GTGAAAAGGG GTCCTCCCCT 
GCTGGGCCCC CAGGCCCTCC CGGCTCACCA GGAGTGAATG GTCAGAAAGG AATCCCGGGA 
GACCCTGCAT TTGGTCACCT GGGACCCCCG GGAAAGAGGG GTCTTTCAGG AGTGCCAGGG 
ATAAAAGGAC CCAGAGGTGA TCCGGGATGT CCAGGGGCTG AAGGGCCAGC TGGCATTCCT 
GGATTCCCAG GTCTCAAAGG TCCCAAAGGC AGAGAGGGAC ATGCTGGGTT TCCAGGTGTC 
CCAGGTCCAC CTGGCCATTC CTGTGAAAGA GGTGCTCCAG GGATACCAGG GCAACCGGGA 
CTCCCTGGGT ATCCAGGTAG CCCAGGTGCT CCAGGTGGGA AAGGACAGCT GGGAGATGTG 
GGGCCTCCCG GGCCAGCTGG AATGAAAGGC CTCCCTGGAC TCCCAGGATG GCCTGGGGCA 
CATGGTCCCC CAGGCCTCCC AGGAATCCCA GGTCCCTTTG GAGATGATGG GCTACCTGGT 
CCTCCAGGTC CAAAGGGACC CCAGGGGCTG CCTGGTTTCC CAGGTTTTCC CGGAGAAAGA 
GGAAAGCCTG GTGCAGAGGG ATGTCCTGGC ACAAAGGGAG AACCTGGAGA GAAGGGCATG 
TCTGGCCTTC CCGGAGACCG GGGACTGAGA GGGGCCAAAG GAGCCATAGG ACCTCCCGGA 
GATGAAGGAG AAATGGCTAT CATTTCCCAA AAGGGAACAC CTGGGGAACC TGGACCTCCT 
GGAGATGATG GATTCCCAGG AGAAAGAGGT GATAAAGGAA CTCCCGGGAT GCAAGGGAGA 
AGAGGAGAGC CGGGAAGATA CGGACCACCT GGATTTCAGA GAGGGGAACC TGGTGAGAAA 
GGTCAGCCAG GGCATCCTGG ACCCCCAGGC CCTCCAGGCT CAACTGGTCT AAGAGGGTTC 
ATTGGTTTTC CAGGACTTCC AGGTGACCAG GGTGAGCCAG GTTCTCCAGG TCCCCCTGGA 
TTTTCAGGAA TTGATGGAGC AAGAGGACCT AAAGGAAACA AAGGTGACCC TGCCAGTCAC 
TTTGGTCCAC CTGGTCCAAA GGGTGAGCCA GGTAGCCCTG GATGTCCAGG GCATTTTGGA 
GCATCTGGAG AGCAGGGCTT GCCTGGTATT CAAGGGCCCA GAGGATCACC TGGAAGGCCA 
GGGCCACCTG GCTCCTCTGG ACCACCAGGG TGCCCAGGTG ATCACGGGAT GCCTGGGCTG 
AGGGGACAGC CAGGAGAAAT GGGAGACCCT GGGCCAAGAG GCCTCCAGGG GGATCCAGGG 
ATACCAGGTC CTCCGGGAAT AAAAGGTCCC TCCGGATCAC CTGGCCTGAA CGGCTTGCAT 
GGATTGAAAG GTCAGAAAGG AACTAAAGGT GCTTCAGGTT TGCATGATGT GGGGCCACCT 
GGTCCAGTGG GAATACCTGG GCTAAAAGGG GAGAGAGGAG ACCCTGGGAG CCCAGGAATC 
TCTCCTCCAG GTCCTCGTGG AGAAAAAGGT CCCCCAGGAC CCCCAGGGAG TTCAGGACCA 
CCTGGTCCTG CAGGTGCCAC AGGAAGAGCT CCTAAGGACA TTCCTGACCC GGGTCCACCT 
GGAGATCAGG GACCTCCTGG TCCTGATGGC CCAAGAGGAG CACCTGGGCC TCCAGGCCTC 
CCTGGGAGTG TTGACCTTCT GAGAGGGGAG CCAGGTGACT GTGGTCTACC AGGGCCACCA 
GGTCCCCCTG GCCCACCAGG CCCTCCAGGA TACAAAGGCT TTCCAGGATG TGATGGAAAA 
GATGGCCAGA AAGGACCAAT GGGATTCCCG GGACCGCAGG GACCACATGG ATTTCCTGGG 
CCACCTGGAG AGAAGGGTTT ACCTGGACCT CCAGGGAGAA AAGGGCCCAC TGGTCTTCCA 
GGTCCCAGAG GTGAACCGGG GCCACCTGCA GATGTGGATG ACTGTCCCCG AATCCCAGGC 
CTTCCTGGGG CGCCAGGCAT GAGAGGACCA GAAGGAGCCA TGGGGCTCTC TGGAATGAGA 
GGCCCCCCAG GACCAGGGTG CAAAGGAGAG CCTGGGCTGG ATGGCAGGAG GGGTGTGGAT 
GGCGTCCCTG GGTCTCCTGG GCCTCCCGGA CGTAAAGGTG ACACAGGAGA AGACGGCTAC 
CCTGGAGGAC CAGGGCCTCC TGGTCCCATT GGGGATCCTG GGCCCAAAGG GTTTGGCCCT 
GGATACCTCG GTGGCTTCCT CCTGGTTCTC CACAGTCAGA CGGACCAGGA GCCCACCTGC 
CCCCTGGGCA TGCCCAGGCT CTGGACTGGG TATAGTCTGT TATACCTGGA AGGGCAAGAG 
AAAGCTCACA ATCAAGACCT TGGTCTGGCA GGGTCTTGCC TTCCCGTGTT TAGCACGCTG 
CCCTTTGCCT ACTGCAACAT CCACCAGGTG TGCCACTATG CCCAGAGAAA CGACAGATCC 
TACTGGCTGG CCAGCGCTGC GCCCCTCCCC ATGATGCCAC TCTCTGAAGA GGCGATCCGC 
CCCTATGTCA GCCGCTGTGC GGTATGCGAG GCCCCGGCCC AGGCGGTGGC GGTGCACAGC 
CAGGACCAGT CCATCCCCCC ATGTCCGCAG ACCTGGAGGA GCCTCTGGAT CGGGTATTCA 
TTCCTGATGC ACACAGGAGC TGGGGACCAA GGAGGAGGGC AGGCCCTTAT GTCACCTGGC 
AGCTGCCTGG AAGATTTCAG AGCAGCACCA TTCCTTGAAT GCCAGGGCCG GCAGGGAACT 
TGCCACTTTT TTGCAAATAA GTATAGCTTC TGGCTCACAA CGGTGAAAGC AGACTTGCAG 
TTTTCCTCTG CTCCAGCACC AGACACCTTA AAAGAAAGCC AGGCCCAACG CCAGAAAATC 
AGCCGGTGCC AGGTCTGCGT GAAGTATAGC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016806080.2
CDS	523..5595
Translation	MWSLHIVLMRCSFRLTKSLATDPWSLTLILFSVQYVYGSGKKYVGPCGGRDCSVCHCVPEKGSRGPPGPPGPQGPIGPLGAPGPIGLSGEKGMRGDRGPPGAAGDKGDKGSTGVPGFPGLDGIPGHPGPPGPRGKPGMSGHNGSRGDPGFPGGRGALGPGGPLGHPGEKGEKGNSVFILGAVKGIQGDRGDPGLPGLPGSWGAGGPAGPTGYPGEPGLVGPPGQPGRPGLKGNPGVGVKGQMGDPGEVGQQGSPGPTLLVEPPDFRLYKGEKGIKGIPGMVGLPGPPGRKGESDIGAKGEKGIPGFPGPRGDPGSYGSPGFPGLKGELGLVGDPGLFGLIGPKGDPGNRGHPGPPGVLVTPPLPLKGPPGDPGFPGRYGETGDVGPPGPPGLLGRPGEACAGMIGPPGPQGFPGLPGLPGEAGIPGRPDSAPGKPGKPGSPGLPGAPGLQGLPGSSVIYCSVGNPGPQGIKGKVGPPGGRGSKGEKGNEGLCACEPGPMGPPGPPGLPGRQGSKGDLGLPGWLGTKGDPGPPGAEGPPGLPGKHGASGPPGNKGAKGDMVVSRVKGHKGERGPDGPPGFPGQPGSHGRDGHAGEKGDPGPPGDHEDATPGGKGFPGPLGPPGKAGPLGPPGLGFPGPPGQRGHPGVPGHPGVRGPDGLKGQKGDTISCNVTYPGRHGPPGFDGPPGPKGFPGPQGAPGLSGSDGHKGRPGTPGTAEIPGPPGFRGDMGDPGFGGEKGSSPAGPPGPPGSPGVNGQKGIPGDPAFGHLGPPGKRGLSGVPGIKGPRGDPGCPGAEGPAGIPGFPGLKGPKGREGHAGFPGVPGPPGHSCERGAPGIPGQPGLPGYPGSPGAPGGKGQLGDVGPPGPAGMKGLPGLPGWPGAHGPPGLPGIPGPFGDDGLPGPPGPKGPQGLPGFPGFPGERGKPGAEGCPGTKGEPGEKGMSGLPGDRGLRGAKGAIGPPGDEGEMAIISQKGTPGEPGPPGDDGFPGERGDKGTPGMQGRRGEPGRYGPPGFQRGEPGEKGQPGHPGPPGPPGSTGLRGFIGFPGLPGDQGEPGSPGPPGFSGIDGARGPKGNKGDPASHFGPPGPKGEPGSPGCPGHFGASGEQGLPGIQGPRGSPGRPGPPGSSGPPGCPGDHGMPGLRGQPGEMGDPGPRGLQGDPGIPGPPGIKGPSGSPGLNGLHGLKGQKGTKGASGLHDVGPPGPVGIPGLKGERGDPGSPGISPPGPRGEKGPPGPPGSSGPPGPAGATGRAPKDIPDPGPPGDQGPPGPDGPRGAPGPPGLPGSVDLLRGEPGDCGLPGPPGPPGPPGPPGYKGFPGCDGKDGQKGPMGFPGPQGPHGFPGPPGEKGLPGPPGRKGPTGLPGPRGEPGPPADVDDCPRIPGLPGAPGMRGPEGAMGLSGMRGPPGPGCKGEPGLDGRRGVDGVPGSPGPPGRKGDTGEDGYPGGPGPPGPIGDPGPKGFGPGYLGGFLLVLHSQTDQEPTCPLGMPRLWTGYSLLYLEGQEKAHNQDLGLAGSCLPVFSTLPFAYCNIHQVCHYAQRNDRSYWLASAAPLPMMPLSEEAIRPYVSRCAVCEAPAQAVAVHSQDQSIPPCPQTWRSLWIGYSFLMHTGAGDQGGGQALMSPGSCLEDFRAAPFLECQGRQGTCHFFANKYSFWLTTVKADLQFSSAPAPDTLKESQAQRQKISRCQVCVKYS

Target ORF information:

RefSeq Version	XM_016950591.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes collagen type IV alpha 4 chain (COL4A4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016950591.2

ORF Insert Sequence:

ATGTGGTCTC TGCACATAGT ACTAATGAGG TGCTCCTTCA GATTGACCAA GTCCTTGGCC 
ACAGATCCCT GGTCACTTAC ACTCATTCTC TTTTCTGTAC AATATGTATA TGGGAGTGGA 
AAGAAATACG TTGGTCCTTG TGGAGGAAGA GATTGCTCTG TTTGCCACTG TGTTCCTGAA 
AAGGGGTCTC GGGGTCCACC AGGACCACCA GGGCCACAGG GTCCAATTGG ACCCCTGGGA 
GCCCCAGGAC CCATTGGGCT TTCAGGAGAG AAAGGAATGA GAGGGGACCG CGGCCCTCCT 
GGAGCAGCAG GGGACAAAGG AGATAAGGGT TCAACTGGTG TTCCTGGATT TCCAGGTTTA 
GATGGCATAC CTGGGCACCC AGGGCCTCCT GGACCCAGAG GCAAACCTGG TATGAGTGGC 
CACAATGGCT CAAGAGGTGA CCCAGGGTTT CCAGGAGGAA GAGGAGCTCT TGGCCCAGGA 
GGCCCCCTAG GCCATCCTGG GGAAAAGGGA GAAAAAGGAA ATTCAGTGTT CATTTTAGGT 
GCCGTTAAAG GTATTCAGGG AGACAGAGGG GACCCAGGAC TGCCTGGCTT ACCAGGATCT 
TGGGGGGCAG GAGGACCGGC GGGTCCCACA GGATATCCTG GAGAGCCAGG GTTAGTGGGA 
CCTCCGGGCC AACCAGGGCG TCCAGGTTTG AAGGGAAATC CCGGTGTGGG AGTAAAGGGG 
CAAATGGGAG ACCCGGGTGA GGTTGGTCAG CAAGGTTCTC CTGGACCCAC CCTGTTGGTG 
GAGCCACCTG ACTTTCGTCT CTATAAAGGA GAAAAGGGTA TAAAAGGAAT TCCTGGAATG 
GTTGGACTGC CAGGACCACC AGGACGCAAG GGAGAATCTG ATATTGGGGC AAAAGGAGAA 
AAAGGTATTC CTGGATTTCC AGGGCCTCGG GGGGATCCTG GTTCCTATGG ATCTCCAGGT 
TTTCCAGGAT TAAAGGGAGA ACTAGGACTG GTTGGAGATC CTGGGCTATT TGGATTAATT 
GGCCCAAAGG GGGATCCTGG AAATCGAGGG CACCCAGGAC CACCAGGTGT TTTGGTGACT 
CCACCTCTTC CACTCAAAGG CCCACCAGGG GACCCAGGGT TCCCTGGCCG CTATGGAGAA 
ACAGGGGATG TTGGACCACC TGGTCCCCCA GGTCTCTTGG GCAGACCAGG GGAAGCCTGT 
GCAGGCATGA TAGGACCCCC TGGGCCACAA GGATTTCCTG GTCTTCCTGG GCTTCCAGGA 
GAAGCTGGTA TTCCTGGGAG ACCTGATTCT GCTCCAGGAA AACCAGGGAA GCCAGGATCA 
CCTGGCTTGC CTGGAGCACC AGGCCTGCAG GGCCTCCCAG GATCAAGTGT GATATACTGT 
AGTGTTGGGA ATCCTGGACC ACAAGGAATA AAAGGCAAAG TGGGTCCCCC AGGAGGAAGA 
GGCTCAAAAG GAGAAAAAGG AAATGAAGGA CTCTGTGCCT GTGAGCCTGG ACCCATGGGC 
CCCCCTGGCC CTCCAGGACT TCCTGGGAGG CAGGGGAGTA AGGGAGACTT GGGGCTCCCT 
GGCTGGCTTG GAACAAAAGG TGACCCAGGA CCTCCTGGTG CTGAAGGACC TCCAGGGCTA 
CCAGGAAAGC ATGGTGCCTC TGGACCACCT GGCAACAAAG GGGCGAAGGG TGACATGGTT 
GTATCAAGAG TTAAAGGGCA CAAAGGAGAA AGAGGTCCTG ATGGGCCCCC AGGATTTCCA 
GGGCAGCCAG GATCACATGG TCGGGATGGA CATGCTGGAG AAAAAGGGGA TCCAGGACCC 
CCAGGGGATC ATGAAGATGC GACCCCAGGT GGTAAAGGAT TTCCTGGACC TCTGGGCCCC 
CCGGGCAAAG CAGGACCTTT GGGGCCCCCA GGACTGGGAT TTCCTGGTCC ACCAGGACAG 
CGAGGCCACC CAGGAGTTCC AGGCCACCCA GGTGTGAGGG GCCCTGATGG CTTGAAGGGT 
CAGAAAGGTG ACACAATTTC TTGCAACGTA ACCTACCCTG GGAGGCACGG CCCTCCAGGT 
TTTGATGGAC CTCCAGGTCC GAAGGGATTT CCAGGTCCCC AAGGTGCCCC TGGGCTGAGT 
GGTTCAGATG GGCATAAAGG CAGACCTGGC ACACCAGGAA CAGCGGAAAT ACCAGGTCCA 
CCTGGTTTTC GTGGTGACAT GGGAGATCCG GGTTTTGGAG GTGAAAAGGG GTCCTCCCCT 
GCTGGGCCCC CAGGCCCTCC CGGCTCACCA GGAGTGAATG GTCAGAAAGG AATCCCGGGA 
GACCCTGCAT TTGGTCACCT GGGACCCCCG GGAAAGAGGG GTCTTTCAGG AGTGCCAGGG 
ATAAAAGGAC CCAGAGGTGA TCCGGGATGT CCAGGGGCTG AAGGGCCAGC TGGCATTCCT 
GGATTCCCAG GTCTCAAAGG TCCCAAAGGC AGAGAGGGAC ATGCTGGGTT TCCAGGTGTC 
CCAGGTCCAC CTGGCCATTC CTGTGAAAGA GGTGCTCCAG GGATACCAGG GCAACCGGGA 
CTCCCTGGGT ATCCAGGTAG CCCAGGTGCT CCAGGTGGGA AAGGACAGCT GGGAGATGTG 
GGGCCTCCCG GGCCAGCTGG AATGAAAGGC CTCCCTGGAC TCCCAGGATG GCCTGGGGCA 
CATGGTCCCC CAGGCCTCCC AGGAATCCCA GGTCCCTTTG GAGATGATGG GCTACCTGGT 
CCTCCAGGTC CAAAGGGACC CCAGGGGCTG CCTGGTTTCC CAGGTTTTCC CGGAGAAAGA 
GGAAAGCCTG GTGCAGAGGG ATGTCCTGGC ACAAAGGGAG AACCTGGAGA GAAGGGCATG 
TCTGGCCTTC CCGGAGACCG GGGACTGAGA GGGGCCAAAG GAGCCATAGG ACCTCCCGGA 
GATGAAGGAG AAATGGCTAT CATTTCCCAA AAGGGAACAC CTGGGGAACC TGGACCTCCT 
GGAGATGATG GATTCCCAGG AGAAAGAGGT GATAAAGGAA CTCCCGGGAT GCAAGGGAGA 
AGAGGAGAGC CGGGAAGATA CGGACCACCT GGATTTCAGA GAGGGGAACC TGGTGAGAAA 
GGTCAGCCAG GGCATCCTGG ACCCCCAGGC CCTCCAGGCT CAACTGGTCT AAGAGGGTTC 
ATTGGTTTTC CAGGACTTCC AGGTGACCAG GGTGAGCCAG GTTCTCCAGG TCCCCCTGGA 
TTTTCAGGAA TTGATGGAGC AAGAGGACCT AAAGGAAACA AAGGTGACCC TGCCAGTCAC 
TTTGGTCCAC CTGGTCCAAA GGGTGAGCCA GGTAGCCCTG GATGTCCAGG GCATTTTGGA 
GCATCTGGAG AGCAGGGCTT GCCTGGTATT CAAGGGCCCA GAGGATCACC TGGAAGGCCA 
GGGCCACCTG GCTCCTCTGG ACCACCAGGG TGCCCAGGTG ATCACGGGAT GCCTGGGCTG 
AGGGGACAGC CAGGAGAAAT GGGAGACCCT GGGCCAAGAG GCCTCCAGGG GGATCCAGGG 
ATACCAGGTC CTCCGGGAAT AAAAGGTCCC TCCGGATCAC CTGGCCTGAA CGGCTTGCAT 
GGATTGAAAG GTCAGAAAGG AACTAAAGGT GCTTCAGGTT TGCATGATGT GGGGCCACCT 
GGTCCAGTGG GAATACCTGG GCTAAAAGGG GAGAGAGGAG ACCCTGGGAG CCCAGGAATC 
TCTCCTCCAG GTCCTCGTGG AGAAAAAGGT CCCCCAGGAC CCCCAGGGAG TTCAGGACCA 
CCTGGTCCTG CAGGTGCCAC AGGAAGAGCT CCTAAGGACA TTCCTGACCC GGGTCCACCT 
GGAGATCAGG GACCTCCTGG TCCTGATGGC CCAAGAGGAG CACCTGGGCC TCCAGGCCTC 
CCTGGGAGTG TTGACCTTCT GAGAGGGGAG CCAGGTGACT GTGGTCTACC AGGGCCACCA 
GGTCCCCCTG GCCCACCAGG CCCTCCAGGA TACAAAGGCT TTCCAGGATG TGATGGAAAA 
GATGGCCAGA AAGGACCAAT GGGATTCCCG GGACCGCAGG GACCACATGG ATTTCCTGGG 
CCACCTGGAG AGAAGGGTTT ACCTGGACCT CCAGGGAGAA AAGGGCCCAC TGGTCTTCCA 
GGTCCCAGAG GTGAACCGGG GCCACCTGCA GATGTGGATG ACTGTCCCCG AATCCCAGGC 
CTTCCTGGGG CGCCAGGCAT GAGAGGACCA GAAGGAGCCA TGGGGCTCTC TGGAATGAGA 
GGCCCCCCAG GACCAGGGTG CAAAGGAGAG CCTGGGCTGG ATGGCAGGAG GGGTGTGGAT 
GGCGTCCCTG GGTCTCCTGG GCCTCCCGGA CGTAAAGGTG ACACAGGAGA AGACGGCTAC 
CCTGGAGGAC CAGGGCCTCC TGGTCCCATT GGGGATCCTG GGCCCAAAGG GTTTGGCCCT 
GGATACCTCG GTGGCTTCCT CCTGGTTCTC CACAGTCAGA CGGACCAGGA GCCCACCTGC 
CCCCTGGGCA TGCCCAGGCT CTGGACTGGG TATAGTCTGT TATACCTGGA AGGGCAAGAG 
AAAGCTCACA ATCAAGACCT TGGTCTGGCA GGGTCTTGCC TTCCCGTGTT TAGCACGCTG 
CCCTTTGCCT ACTGCAACAT CCACCAGGTG TGCCACTATG CCCAGAGAAA CGACAGATCC 
TACTGGCTGG CCAGCGCTGC GCCCCTCCCC ATGATGCCAC TCTCTGAAGA GGCGATCCGC 
CCCTATGTCA GCCGCTGTGC GGTATGCGAG GCCCCGGCCC AGGCGGTGGC GGTGCACAGC 
CAGGACCAGT CCATCCCCCC ATGTCCGCAG ACCTGGAGGA GCCTCTGGAT CGGGTATTCA 
TTCCTGATGC ACACAGGAGC TGGGGACCAA GGAGGAGGGC AGGCCCTTAT GTCACCTGGC 
AGCTGCCTGG AAGATTTCAG AGCAGCACCA TTCCTTGAAT GCCAGGGCCG GCAGGGAACT 
TGCCACTTTT TTGCAAATAA GTATAGCTTC TGGCTCACAA CGGTGAAAGC AGACTTGCAG 
TTTTCCTCTG CTCCAGCACC AGACACCTTA AAAGAAAGCC AGGCCCAACG CCAGAAAATC 
AGCCGGTGCC AGGTCTGCGT GAAGTATAGC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL4A4 ( XM_009444466.3 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_009442741.3 Pan troglodytes collagen type IV alpha 4 chain (COL4A4), transcript variant X5, mRNA.

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CloneID	OPc104270
Clone ID Related Accession (Same CDS sequence)	XM_009444466.3
Accession Version	XM_009444466.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5028bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	collagen alpha-4(IV) chain isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036881.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_009444466.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGTCTC TGCACATAGT ACTAATGAGG TGCTCCTTCA GATTGACCAA GTCCTTGGCC 
ACAGATCCCT GGTCACTTAC ACTCATTCTC TTTTCTGTAC AATATGTATA TGGGAGTGGA 
AAGAAATACG TTGGTCCTTG TGGAGGAAGA GATTGCTCTG TTTGCCACTG TGTTCCTGAA 
AAGGGGTCTC GGGGTCCACC AGGACCACCA GGGCCACAGG GTCCAATTGG ACCCCTGGGA 
GCCCCAGGAC CCATTGGGCT TTCAGGAGAG AAAGGAATGA GAGGGGACCG CGGCCCTCCT 
GGAGCAGCAG GGGACAAAGG AGATAAGGGT TCAACTGGTG TTCCTGGATT TCCAGGTTTA 
GATGGCATAC CTGGGCACCC AGGGCCTCCT GGACCCAGAG GCAAACCTGG TATGAGTGGC 
CACAATGGCT CAAGAGGTGA CCCAGGGTTT CCAGGAGGAA GAGGAGCTCT TGGCCCAGGA 
GGCCCCCTAG GCCATCCTGG GGAAAAGGGA GAAAAAGGAA ATTCAGTGTT CATTTTAGGT 
GCCGTTAAAG GTATTCAGGG AGACAGAGGG GACCCAGGAC TGCCTGGCTT ACCAGGATCT 
TGGGGGGCAG GAGGACCGGC GGGTCCCACA GGATATCCTG GAGAGCCAGG GTTAGTGGGA 
CCTCCGGGCC AACCAGGGCG TCCAGGTTTG AAGGGAAATC CCGGTGTGGG AGTAAAGGGG 
CAAATGGGAG ACCCGGGTGA GGTTGGTCAG CAAGGTTCTC CTGGACCCAC CCTGTTGGTG 
GAGCCACCTG ACTTTCGTCT CTATAAAGGA GAAAAGGGTA TAAAAGGAAT TCCTGGAATG 
GTTGGACTGC CAGGACCACC AGGACGCAAG GGAGAATCTG ATATTGGGGC AAAAGGAGAA 
AAAGGTATTC CTGGATTTCC AGGGCCTCGG GGAGAACTAG GACTGGTTGG AGATCCTGGG 
CTATTTGGAT TAATTGGCCC AAAGGGGGAT CCTGGAAATC GAGGGCACCC AGGACCACCA 
GGTGTTTTGG TGACTCCACC TCTTCCACTC AAAGGCCCAC CAGGGGACCC AGGGTTCCCT 
GGCCGCTATG GAGAAACAGG GGATGTTGGA CCACCTGGTC CCCCAGGTCT CTTGGGCAGA 
CCAGGGGAAG CCTGTGCAGG CATGATAGGA CCCCCTGGGC CACAAGGATT TCCTGGTCTT 
CCTGGGCTTC CAGGAGAAGC TGGTATTCCT GGGAGACCTG ATTCTGCTCC AGGAAAACCA 
GGGAAGCCAG GATCACCTGG CTTGCCTGGA GCACCAGGCC TGCAGGGCCT CCCAGGATCA 
AGTGTGATAT ACTGTAGTGT TGGGAATCCT GGACCACAAG GAATAAAAGG CAAAGTGGGT 
CCCCCAGGAG GAAGAGGCTC AAAAGGAGAA AAAGGAAATG AAGGACTCTG TGCCTGTGAG 
CCTGGACCCA TGGGCCCCCC TGGCCCTCCA GGACTTCCTG GGAGGCAGGG GAGTAAGGGA 
GACTTGGGGC TCCCTGGCTG GCTTGGAACA AAAGGTGACC CAGGACCTCC TGGTGCTGAA 
GGACCTCCAG GGCTACCAGG AAAGCATGGT GCCTCTGGAC CACCTGGCAA CAAAGGGGCG 
AAGGGTGACA TGGTTGTATC AAGAGTTAAA GGGCACAAAG GAGAAAGAGG TCCTGATGGG 
CCCCCAGGAT TTCCAGGGCA GCCAGGATCA CATGGTCGGG ATGGACATGC TGGAGAAAAA 
GGGGATCCAG GACCCCCAGG GGATCATGAA GATGCGACCC CAGGTGGTAA AGGATTTCCT 
GGACCTCTGG GCCCCCCGGG CAAAGCAGGA CCTTTGGGGC CCCCAGGACT GGGATTTCCT 
GGTCCACCAG GACAGCGAGG CCACCCAGGA GTTCCAGGCC ACCCAGGTGT GAGGGGCCCT 
GATGGCTTGA AGGGTCAGAA AGGTGACACA ATTTCTTGCA ACGTAACCTA CCCTGGGAGG 
CACGGCCCTC CAGGTTTTGA TGGACCTCCA GGTCCGAAGG GATTTCCAGG TCCCCAAGGT 
GCCCCTGGGC TGAGTGGTTC AGATGGGCAT AAAGGCAGAC CTGGCACACC AGGAACAGCG 
GAAATACCAG GTCCACCTGG TTTTCGTGGT GACATGGGAG ATCCGGGTTT TGGAGGTGAA 
AAGGGGTCCT CCCCTGCTGG GCCCCCAGGC CCTCCCGGCT CACCAGGAGT GAATGGTCAG 
AAAGGAATCC CGGGAGACCC TGCATTTGGT CACCTGGGAC CCCCGGGAAA GAGGGGTCTT 
TCAGGAGTGC CAGGGATAAA AGGACCCAGA GGTGATCCGG GATGTCCAGG GGCTGAAGGG 
CCAGCTGGCA TTCCTGGATT CCCAGGTCTC AAAGGTCCCA AAGGCAGAGA GGGACATGCT 
GGGTTTCCAG GTGTCCCAGG TCCACCTGGC CATTCCTGTG AAAGAGGTGC TCCAGGGATA 
CCAGGGCAAC CGGGACTCCC TGGGTATCCA GGTAGCCCAG GTGCTCCAGG TGGGAAAGGA 
CAGCTGGGAG ATGTGGGGCC TCCCGGGCCA GCTGGAATGA AAGGCCTCCC TGGACTCCCA 
GGATGGCCTG GGGCACATGG TCCCCCAGGC CTCCCAGGAA TCCCAGGTCC CTTTGGAGAT 
GATGGGCTAC CTGGTCCTCC AGGTCCAAAG GGACCCCAGG GGCTGCCTGG TTTCCCAGGT 
TTTCCCGGAG AAAGAGGAAA GCCTGGTGCA GAGGGATGTC CTGGCACAAA GGGAGAACCT 
GGAGAGAAGG GCATGTCTGG CCTTCCCGGA GACCGGGGAC TGAGAGGGGC CAAAGGAGCC 
ATAGGACCTC CCGGAGATGA AGGAGAAATG GCTATCATTT CCCAAAAGGG AACACCTGGG 
GAACCTGGAC CTCCTGGAGA TGATGGATTC CCAGGAGAAA GAGGTGATAA AGGAACTCCC 
GGGATGCAAG GGAGAAGAGG AGAGCCGGGA AGATACGGAC CACCTGGATT TCAGAGAGGG 
GAACCTGGTG AGAAAGGTCA GCCAGGGCAT CCTGGACCCC CAGGCCCTCC AGGCTCAACT 
GGTCTAAGAG GGTTCATTGG TTTTCCAGGA CTTCCAGGTG ACCAGGGTGA GCCAGGTTCT 
CCAGGTCCCC CTGGATTTTC AGGAATTGAT GGAGCAAGAG GACCTAAAGG AAACAAAGGT 
GACCCTGCCA GTCACTTTGG TCCACCTGGT CCAAAGGGTG AGCCAGGTAG CCCTGGATGT 
CCAGGGCATT TTGGAGCATC TGGAGAGCAG GGCTTGCCTG GTATTCAAGG GCCCAGAGGA 
TCACCTGGAA GGCCAGGGCC ACCTGGCTCC TCTGGACCAC CAGGGTGCCC AGGTGATCAC 
GGGATGCCTG GGCTGAGGGG ACAGCCAGGA GAAATGGGAG ACCCTGGGCC AAGAGGCCTC 
CAGGGGGATC CAGGGATACC AGGTCCTCCG GGAATAAAAG GTCCCTCCGG ATCACCTGGC 
CTGAACGGCT TGCATGGATT GAAAGGTCAG AAAGGAACTA AAGGTGCTTC AGGTTTGCAT 
GATGTGGGGC CACCTGGTCC AGTGGGAATA CCTGGGCTAA AAGGGGAGAG AGGAGACCCT 
GGGAGCCCAG GAATCTCTCC TCCAGGTCCT CGTGGAGAAA AAGGTCCCCC AGGACCCCCA 
GGGAGTTCAG GACCACCTGG TCCTGCAGGT GCCACAGGAA GAGCTCCTAA GGACATTCCT 
GACCCGGGTC CACCTGGAGA TCAGGGACCT CCTGGTCCTG ATGGCCCAAG AGGAGCACCT 
GGGCCTCCAG GCCTCCCTGG GAGTGTTGAC CTTCTGAGAG GGGAGCCAGG TGACTGTGGT 
CTACCAGGGC CACCAGGTCC CCCTGGCCCA CCAGGCCCTC CAGGATACAA AGGCTTTCCA 
GGATGTGATG GAAAAGATGG CCAGAAAGGA CCAATGGGAT TCCCGGGACC GCAGGGACCA 
CATGGATTTC CTGGGCCACC TGGAGAGAAG GGTTTACCTG GACCTCCAGG GAGAAAAGGG 
CCCACTGGTC TTCCAGGTCC CAGAGGTGAA CCGGGGCCAC CTGCAGATGT GGATGACTGT 
CCCCGAATCC CAGGCCTTCC TGGGGCGCCA GGCATGAGAG GACCAGAAGG AGCCATGGGG 
CTCTCTGGAA TGAGAGGCCC CCCAGGACCA GGGTGCAAAG GAGAGCCTGG GCTGGATGGC 
AGGAGGGGTG TGGATGGCGT CCCTGGGTCT CCTGGGCCTC CCGGACGTAA AGGTGACACA 
GGAGAAGACG GCTACCCTGG AGGACCAGGG CCTCCTGGTC CCATTGGGGA TCCTGGGCCC 
AAAGGGTTTG GCCCTGGATA CCTCGGTGGC TTCCTCCTGG TTCTCCACAG TCAGACGGAC 
CAGGAGCCCA CCTGCCCCCT GGGCATGCCC AGGCTCTGGA CTGGGTATAG TCTGTTATAC 
CTGGAAGGGC AAGAGAAAGC TCACAATCAA GACCTTGGTC TGGCAGGGTC TTGCCTTCCC 
GTGTTTAGCA CGCTGCCCTT TGCCTACTGC AACATCCACC AGGTGTGCCA CTATGCCCAG 
AGAAACGACA GATCCTACTG GCTGGCCAGC GCTGCGCCCC TCCCCATGAT GCCACTCTCT 
GAAGAGGCGA TCCGCCCCTA TGTCAGCCGC TGTGCGGTAT GCGAGGCCCC GGCCCAGGCG 
GTGGCGGTGC ACAGCCAGGA CCAGTCCATC CCCCCATGTC CGCAGACCTG GAGGAGCCTC 
TGGATCGGGT ATTCATTCCT GATGCACACA GGAGCTGGGG ACCAAGGAGG AGGGCAGGCC 
CTTATGTCAC CTGGCAGCTG CCTGGAAGAT TTCAGAGCAG CACCATTCCT TGAATGCCAG 
GGCCGGCAGG GAACTTGCCA CTTTTTTGCA AATAAGTATA GCTTCTGGCT CACAACGGTG 
AAAGCAGACT TGCAGTTTTC CTCTGCTCCA GCACCAGACA CCTTAAAAGA AAGCCAGGCC 
CAACGCCAGA AAATCAGCCG GTGCCAGGTC TGCGTGAAGT ATAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009442741.3
CDS	425..5452
Translation	MWSLHIVLMRCSFRLTKSLATDPWSLTLILFSVQYVYGSGKKYVGPCGGRDCSVCHCVPEKGSRGPPGPPGPQGPIGPLGAPGPIGLSGEKGMRGDRGPPGAAGDKGDKGSTGVPGFPGLDGIPGHPGPPGPRGKPGMSGHNGSRGDPGFPGGRGALGPGGPLGHPGEKGEKGNSVFILGAVKGIQGDRGDPGLPGLPGSWGAGGPAGPTGYPGEPGLVGPPGQPGRPGLKGNPGVGVKGQMGDPGEVGQQGSPGPTLLVEPPDFRLYKGEKGIKGIPGMVGLPGPPGRKGESDIGAKGEKGIPGFPGPRGELGLVGDPGLFGLIGPKGDPGNRGHPGPPGVLVTPPLPLKGPPGDPGFPGRYGETGDVGPPGPPGLLGRPGEACAGMIGPPGPQGFPGLPGLPGEAGIPGRPDSAPGKPGKPGSPGLPGAPGLQGLPGSSVIYCSVGNPGPQGIKGKVGPPGGRGSKGEKGNEGLCACEPGPMGPPGPPGLPGRQGSKGDLGLPGWLGTKGDPGPPGAEGPPGLPGKHGASGPPGNKGAKGDMVVSRVKGHKGERGPDGPPGFPGQPGSHGRDGHAGEKGDPGPPGDHEDATPGGKGFPGPLGPPGKAGPLGPPGLGFPGPPGQRGHPGVPGHPGVRGPDGLKGQKGDTISCNVTYPGRHGPPGFDGPPGPKGFPGPQGAPGLSGSDGHKGRPGTPGTAEIPGPPGFRGDMGDPGFGGEKGSSPAGPPGPPGSPGVNGQKGIPGDPAFGHLGPPGKRGLSGVPGIKGPRGDPGCPGAEGPAGIPGFPGLKGPKGREGHAGFPGVPGPPGHSCERGAPGIPGQPGLPGYPGSPGAPGGKGQLGDVGPPGPAGMKGLPGLPGWPGAHGPPGLPGIPGPFGDDGLPGPPGPKGPQGLPGFPGFPGERGKPGAEGCPGTKGEPGEKGMSGLPGDRGLRGAKGAIGPPGDEGEMAIISQKGTPGEPGPPGDDGFPGERGDKGTPGMQGRRGEPGRYGPPGFQRGEPGEKGQPGHPGPPGPPGSTGLRGFIGFPGLPGDQGEPGSPGPPGFSGIDGARGPKGNKGDPASHFGPPGPKGEPGSPGCPGHFGASGEQGLPGIQGPRGSPGRPGPPGSSGPPGCPGDHGMPGLRGQPGEMGDPGPRGLQGDPGIPGPPGIKGPSGSPGLNGLHGLKGQKGTKGASGLHDVGPPGPVGIPGLKGERGDPGSPGISPPGPRGEKGPPGPPGSSGPPGPAGATGRAPKDIPDPGPPGDQGPPGPDGPRGAPGPPGLPGSVDLLRGEPGDCGLPGPPGPPGPPGPPGYKGFPGCDGKDGQKGPMGFPGPQGPHGFPGPPGEKGLPGPPGRKGPTGLPGPRGEPGPPADVDDCPRIPGLPGAPGMRGPEGAMGLSGMRGPPGPGCKGEPGLDGRRGVDGVPGSPGPPGRKGDTGEDGYPGGPGPPGPIGDPGPKGFGPGYLGGFLLVLHSQTDQEPTCPLGMPRLWTGYSLLYLEGQEKAHNQDLGLAGSCLPVFSTLPFAYCNIHQVCHYAQRNDRSYWLASAAPLPMMPLSEEAIRPYVSRCAVCEAPAQAVAVHSQDQSIPPCPQTWRSLWIGYSFLMHTGAGDQGGGQALMSPGSCLEDFRAAPFLECQGRQGTCHFFANKYSFWLTTVKADLQFSSAPAPDTLKESQAQRQKISRCQVCVKYS

Target ORF information:

RefSeq Version	XM_009444466.3
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes collagen type IV alpha 4 chain (COL4A4), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009444466.3

ORF Insert Sequence:

ATGTGGTCTC TGCACATAGT ACTAATGAGG TGCTCCTTCA GATTGACCAA GTCCTTGGCC 
ACAGATCCCT GGTCACTTAC ACTCATTCTC TTTTCTGTAC AATATGTATA TGGGAGTGGA 
AAGAAATACG TTGGTCCTTG TGGAGGAAGA GATTGCTCTG TTTGCCACTG TGTTCCTGAA 
AAGGGGTCTC GGGGTCCACC AGGACCACCA GGGCCACAGG GTCCAATTGG ACCCCTGGGA 
GCCCCAGGAC CCATTGGGCT TTCAGGAGAG AAAGGAATGA GAGGGGACCG CGGCCCTCCT 
GGAGCAGCAG GGGACAAAGG AGATAAGGGT TCAACTGGTG TTCCTGGATT TCCAGGTTTA 
GATGGCATAC CTGGGCACCC AGGGCCTCCT GGACCCAGAG GCAAACCTGG TATGAGTGGC 
CACAATGGCT CAAGAGGTGA CCCAGGGTTT CCAGGAGGAA GAGGAGCTCT TGGCCCAGGA 
GGCCCCCTAG GCCATCCTGG GGAAAAGGGA GAAAAAGGAA ATTCAGTGTT CATTTTAGGT 
GCCGTTAAAG GTATTCAGGG AGACAGAGGG GACCCAGGAC TGCCTGGCTT ACCAGGATCT 
TGGGGGGCAG GAGGACCGGC GGGTCCCACA GGATATCCTG GAGAGCCAGG GTTAGTGGGA 
CCTCCGGGCC AACCAGGGCG TCCAGGTTTG AAGGGAAATC CCGGTGTGGG AGTAAAGGGG 
CAAATGGGAG ACCCGGGTGA GGTTGGTCAG CAAGGTTCTC CTGGACCCAC CCTGTTGGTG 
GAGCCACCTG ACTTTCGTCT CTATAAAGGA GAAAAGGGTA TAAAAGGAAT TCCTGGAATG 
GTTGGACTGC CAGGACCACC AGGACGCAAG GGAGAATCTG ATATTGGGGC AAAAGGAGAA 
AAAGGTATTC CTGGATTTCC AGGGCCTCGG GGAGAACTAG GACTGGTTGG AGATCCTGGG 
CTATTTGGAT TAATTGGCCC AAAGGGGGAT CCTGGAAATC GAGGGCACCC AGGACCACCA 
GGTGTTTTGG TGACTCCACC TCTTCCACTC AAAGGCCCAC CAGGGGACCC AGGGTTCCCT 
GGCCGCTATG GAGAAACAGG GGATGTTGGA CCACCTGGTC CCCCAGGTCT CTTGGGCAGA 
CCAGGGGAAG CCTGTGCAGG CATGATAGGA CCCCCTGGGC CACAAGGATT TCCTGGTCTT 
CCTGGGCTTC CAGGAGAAGC TGGTATTCCT GGGAGACCTG ATTCTGCTCC AGGAAAACCA 
GGGAAGCCAG GATCACCTGG CTTGCCTGGA GCACCAGGCC TGCAGGGCCT CCCAGGATCA 
AGTGTGATAT ACTGTAGTGT TGGGAATCCT GGACCACAAG GAATAAAAGG CAAAGTGGGT 
CCCCCAGGAG GAAGAGGCTC AAAAGGAGAA AAAGGAAATG AAGGACTCTG TGCCTGTGAG 
CCTGGACCCA TGGGCCCCCC TGGCCCTCCA GGACTTCCTG GGAGGCAGGG GAGTAAGGGA 
GACTTGGGGC TCCCTGGCTG GCTTGGAACA AAAGGTGACC CAGGACCTCC TGGTGCTGAA 
GGACCTCCAG GGCTACCAGG AAAGCATGGT GCCTCTGGAC CACCTGGCAA CAAAGGGGCG 
AAGGGTGACA TGGTTGTATC AAGAGTTAAA GGGCACAAAG GAGAAAGAGG TCCTGATGGG 
CCCCCAGGAT TTCCAGGGCA GCCAGGATCA CATGGTCGGG ATGGACATGC TGGAGAAAAA 
GGGGATCCAG GACCCCCAGG GGATCATGAA GATGCGACCC CAGGTGGTAA AGGATTTCCT 
GGACCTCTGG GCCCCCCGGG CAAAGCAGGA CCTTTGGGGC CCCCAGGACT GGGATTTCCT 
GGTCCACCAG GACAGCGAGG CCACCCAGGA GTTCCAGGCC ACCCAGGTGT GAGGGGCCCT 
GATGGCTTGA AGGGTCAGAA AGGTGACACA ATTTCTTGCA ACGTAACCTA CCCTGGGAGG 
CACGGCCCTC CAGGTTTTGA TGGACCTCCA GGTCCGAAGG GATTTCCAGG TCCCCAAGGT 
GCCCCTGGGC TGAGTGGTTC AGATGGGCAT AAAGGCAGAC CTGGCACACC AGGAACAGCG 
GAAATACCAG GTCCACCTGG TTTTCGTGGT GACATGGGAG ATCCGGGTTT TGGAGGTGAA 
AAGGGGTCCT CCCCTGCTGG GCCCCCAGGC CCTCCCGGCT CACCAGGAGT GAATGGTCAG 
AAAGGAATCC CGGGAGACCC TGCATTTGGT CACCTGGGAC CCCCGGGAAA GAGGGGTCTT 
TCAGGAGTGC CAGGGATAAA AGGACCCAGA GGTGATCCGG GATGTCCAGG GGCTGAAGGG 
CCAGCTGGCA TTCCTGGATT CCCAGGTCTC AAAGGTCCCA AAGGCAGAGA GGGACATGCT 
GGGTTTCCAG GTGTCCCAGG TCCACCTGGC CATTCCTGTG AAAGAGGTGC TCCAGGGATA 
CCAGGGCAAC CGGGACTCCC TGGGTATCCA GGTAGCCCAG GTGCTCCAGG TGGGAAAGGA 
CAGCTGGGAG ATGTGGGGCC TCCCGGGCCA GCTGGAATGA AAGGCCTCCC TGGACTCCCA 
GGATGGCCTG GGGCACATGG TCCCCCAGGC CTCCCAGGAA TCCCAGGTCC CTTTGGAGAT 
GATGGGCTAC CTGGTCCTCC AGGTCCAAAG GGACCCCAGG GGCTGCCTGG TTTCCCAGGT 
TTTCCCGGAG AAAGAGGAAA GCCTGGTGCA GAGGGATGTC CTGGCACAAA GGGAGAACCT 
GGAGAGAAGG GCATGTCTGG CCTTCCCGGA GACCGGGGAC TGAGAGGGGC CAAAGGAGCC 
ATAGGACCTC CCGGAGATGA AGGAGAAATG GCTATCATTT CCCAAAAGGG AACACCTGGG 
GAACCTGGAC CTCCTGGAGA TGATGGATTC CCAGGAGAAA GAGGTGATAA AGGAACTCCC 
GGGATGCAAG GGAGAAGAGG AGAGCCGGGA AGATACGGAC CACCTGGATT TCAGAGAGGG 
GAACCTGGTG AGAAAGGTCA GCCAGGGCAT CCTGGACCCC CAGGCCCTCC AGGCTCAACT 
GGTCTAAGAG GGTTCATTGG TTTTCCAGGA CTTCCAGGTG ACCAGGGTGA GCCAGGTTCT 
CCAGGTCCCC CTGGATTTTC AGGAATTGAT GGAGCAAGAG GACCTAAAGG AAACAAAGGT 
GACCCTGCCA GTCACTTTGG TCCACCTGGT CCAAAGGGTG AGCCAGGTAG CCCTGGATGT 
CCAGGGCATT TTGGAGCATC TGGAGAGCAG GGCTTGCCTG GTATTCAAGG GCCCAGAGGA 
TCACCTGGAA GGCCAGGGCC ACCTGGCTCC TCTGGACCAC CAGGGTGCCC AGGTGATCAC 
GGGATGCCTG GGCTGAGGGG ACAGCCAGGA GAAATGGGAG ACCCTGGGCC AAGAGGCCTC 
CAGGGGGATC CAGGGATACC AGGTCCTCCG GGAATAAAAG GTCCCTCCGG ATCACCTGGC 
CTGAACGGCT TGCATGGATT GAAAGGTCAG AAAGGAACTA AAGGTGCTTC AGGTTTGCAT 
GATGTGGGGC CACCTGGTCC AGTGGGAATA CCTGGGCTAA AAGGGGAGAG AGGAGACCCT 
GGGAGCCCAG GAATCTCTCC TCCAGGTCCT CGTGGAGAAA AAGGTCCCCC AGGACCCCCA 
GGGAGTTCAG GACCACCTGG TCCTGCAGGT GCCACAGGAA GAGCTCCTAA GGACATTCCT 
GACCCGGGTC CACCTGGAGA TCAGGGACCT CCTGGTCCTG ATGGCCCAAG AGGAGCACCT 
GGGCCTCCAG GCCTCCCTGG GAGTGTTGAC CTTCTGAGAG GGGAGCCAGG TGACTGTGGT 
CTACCAGGGC CACCAGGTCC CCCTGGCCCA CCAGGCCCTC CAGGATACAA AGGCTTTCCA 
GGATGTGATG GAAAAGATGG CCAGAAAGGA CCAATGGGAT TCCCGGGACC GCAGGGACCA 
CATGGATTTC CTGGGCCACC TGGAGAGAAG GGTTTACCTG GACCTCCAGG GAGAAAAGGG 
CCCACTGGTC TTCCAGGTCC CAGAGGTGAA CCGGGGCCAC CTGCAGATGT GGATGACTGT 
CCCCGAATCC CAGGCCTTCC TGGGGCGCCA GGCATGAGAG GACCAGAAGG AGCCATGGGG 
CTCTCTGGAA TGAGAGGCCC CCCAGGACCA GGGTGCAAAG GAGAGCCTGG GCTGGATGGC 
AGGAGGGGTG TGGATGGCGT CCCTGGGTCT CCTGGGCCTC CCGGACGTAA AGGTGACACA 
GGAGAAGACG GCTACCCTGG AGGACCAGGG CCTCCTGGTC CCATTGGGGA TCCTGGGCCC 
AAAGGGTTTG GCCCTGGATA CCTCGGTGGC TTCCTCCTGG TTCTCCACAG TCAGACGGAC 
CAGGAGCCCA CCTGCCCCCT GGGCATGCCC AGGCTCTGGA CTGGGTATAG TCTGTTATAC 
CTGGAAGGGC AAGAGAAAGC TCACAATCAA GACCTTGGTC TGGCAGGGTC TTGCCTTCCC 
GTGTTTAGCA CGCTGCCCTT TGCCTACTGC AACATCCACC AGGTGTGCCA CTATGCCCAG 
AGAAACGACA GATCCTACTG GCTGGCCAGC GCTGCGCCCC TCCCCATGAT GCCACTCTCT 
GAAGAGGCGA TCCGCCCCTA TGTCAGCCGC TGTGCGGTAT GCGAGGCCCC GGCCCAGGCG 
GTGGCGGTGC ACAGCCAGGA CCAGTCCATC CCCCCATGTC CGCAGACCTG GAGGAGCCTC 
TGGATCGGGT ATTCATTCCT GATGCACACA GGAGCTGGGG ACCAAGGAGG AGGGCAGGCC 
CTTATGTCAC CTGGCAGCTG CCTGGAAGAT TTCAGAGCAG CACCATTCCT TGAATGCCAG 
GGCCGGCAGG GAACTTGCCA CTTTTTTGCA AATAAGTATA GCTTCTGGCT CACAACGGTG 
AAAGCAGACT TGCAGTTTTC CTCTGCTCCA GCACCAGACA CCTTAAAAGA AAGCCAGGCC 
CAACGCCAGA AAATCAGCCG GTGCCAGGTC TGCGTGAAGT ATAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL4A4 ( XM_009444467.3 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_009442742.3 Pan troglodytes collagen type IV alpha 4 chain (COL4A4), transcript variant X6, mRNA.

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CloneID	OPc104271
Clone ID Related Accession (Same CDS sequence)	XM_009444467.3
Accession Version	XM_009444467.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4992bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	collagen alpha-4(IV) chain isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036881.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_009444467.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGTCTC TGCACATAGT ACTAATGAGG TGCTCCTTCA GATTGACCAA GTCCTTGGCC 
ACAGATCCCT GGTCACTTAC ACTCATTCTC TTTTCTGTAC AATATGTATA TGGGAGTGGA 
AAGAAATACG TTGGTCCTTG TGGAGGAAGA GATTGCTCTG TTTGCCACTG TGTTCCTGAA 
AAGGGGTCTC GGGGTCCACC AGGACCACCA GGGCCACAGG GTCCAATTGG ACCCCTGGGA 
GCCCCAGGAC CCATTGGGCT TTCAGGAGAG AAAGGAATGA GAGGGGACCG CGGCCCTCCT 
GGAGCAGCAG GGGACAAAGG AGATAAGGGT TCAACTGGTG TTCCTGGATT TCCAGGTTTA 
GATGGCATAC CTGGGCACCC AGGGCCTCCT GGACCCAGAG GCAAACCTGG TATGAGTGGC 
CACAATGGCT CAAGAGGTGA CCCAGGGTTT CCAGGAGGAA GAGGAGCTCT TGGCCCAGGA 
GGCCCCCTAG GCCATCCTGG GGAAAAGGGA GAAAAAGGAA ATTCAGTGTT CATTTTAGGT 
GCCGTTAAAG GTATTCAGGG AGACAGAGGG GACCCAGGAC TGCCTGGCTT ACCAGGATCT 
TGGGGGGCAG GAGGACCGGC GGGTCCCACA GGATATCCTG GAGAGCCAGG GTTAGTGGGA 
CCTCCGGGCC AACCAGGGCG TCCAGGTTTG AAGGGAAATC CCGGTGTGGG AGTAAAGGGG 
CAAATGGGAG ACCCGGGTGA GGTTGGTCAG CAAGGTTCTC CTGGACCCAC CCTGTTGGTG 
GAGCCACCTG ACTTTCGTCT CTATAAAGGA GAAAAGGGTA TAAAAGGAAT TCCTGGAATG 
GTTGGACTGC CAGGACCACC AGGACGCAAG GGAGAATCTG ATATTGGGGC AAAAGGAGAA 
AAAGGTATTC CTGGATTTCC AGGGCCTCGG GGGGATCCTG GTTCCTATGG ATCTCCAGGT 
TTTCCAGGAT TAAAGGGAGA ACTAGGACTG GTTGGAGATC CTGGGCTATT TGGATTAATT 
GGCCCAAAGG GGGATCCTGG AAATCGAGGG CACCCAGGAC CACCAGGTGT TTTGGTGACT 
CCACCTCTTC CACTCAAAGG CCCACCAGGG GACCCAGGGT TCCCTGGCCG CTATGGAGAA 
ACAGGGGATG TTGGACCACC TGGTCCCCCA GGTCTCTTGG GCAGACCAGG GGAAGCCTGT 
GCAGGCATGA TAGGACCCCC TGGGCCACAA GGATTTCCTG GTCTTCCTGG GCTTCCAGGA 
GAAGCTGGTA TTCCTGGGAG ACCTGATTCT GCTCCAGGAA AACCAGGGAA GCCAGGATCA 
CCTGGCTTGC CTGGAGCACC AGGCCTGCAG GGCCTCCCAG GATCAAGTGT GATATACTGT 
AGTGTTGGGA ATCCTGGACC ACAAGGAATA AAAGGCAAAG TGGGTCCCCC AGGAGGAAGA 
GGCTCAAAAG GAGAAAAAGG AAATGAAGGA CTCTGTGCCT GTGAGCCTGG ACCCATGGGC 
CCCCCTGGCC CTCCAGGACT TCCTGGGAGG CAGGGGAGTA AGGGAGACTT GGGGCTCCCT 
GGCTGGCTTG GAACAAAAGG TGACCCAGGA CCTCCTGGTG CTGAAGGACC TCCAGGGCTA 
CCAGGAAAGC ATGGTGCCTC TGGACCACCT GGCAACAAAG GGGCGAAGGG TGACATGGTT 
GTATCAAGAG TTAAAGGGCA CAAAGGAGAA AGAGGTCCTG ATGGGCCCCC AGGATTTCCA 
GGGCAGCCAG GATCACATGG TCGGGATGGA CATGCTGGAG AAAAAGGGGA TCCAGGACCC 
CCAGGGGATC ATGAAGATGC GACCCCAGGT GGTAAAGGAT TTCCTGGACC TCTGGGCCCC 
CCGGGCAAAG CAGGACCTTT GGGGCCCCCA GGACTGGGAT TTCCTGGTCC ACCAGGACAG 
CGAGGCCACC CAGGAGTTCC AGGCCACCCA GGTGTGAGGG GCCCTGATGG CTTGAAGGGT 
CAGAAAGGTG ACACAATTTC TTGCAACGTA ACCTACCCTG GGAGGCACGG CCCTCCAGGT 
TTTGATGGAC CTCCAGGTCC GAAGGGATTT CCAGGTCCCC AAGGTGCCCC TGGGCTGAGT 
GGTTCAGATG GGCATAAAGG CAGACCTGGC ACACCAGGAA CAGCGGAAAT ACCAGGTCCA 
CCTGGTTTTC GTGGTGACAT GGGAGATCCG GGTTTTGGAG GTGAAAAGGG GTCCTCCCCT 
GCTGGGCCCC CAGGCCCTCC CGGCTCACCA GGAGTGAATG GTCAGAAAGG AATCCCGGGA 
GACCCTGCAT TTGGTCACCT GGGACCCCCG GGAAAGAGGG GTCTTTCAGG AGTGCCAGGG 
ATAAAAGGAC CCAGAGGTGA TCCGGGATGT CCAGGGGCTG AAGGGCCAGC TGGCATTCCT 
GGATTCCCAG GTCTCAAAGG TCCCAAAGGC AGAGAGGGAC ATGCTGGGTT TCCAGGTGTC 
CCAGGTCCAC CTGGCCATTC CTGTGAAAGA GGTGCTCCAG GGATACCAGG GCAACCGGGA 
CTCCCTGGGT ATCCAGGTAG CCCAGGTGCT CCAGGTGGGA AAGGACAGCT GGGAGATGTG 
GGGCCTCCCG GGCCAGCTGG AATGAAAGGC CTCCCTGGAC TCCCAGGATG GCCTGGGGCA 
CATGGTCCCC CAGGCCTCCC AGGAATCCCA GGTCCCTTTG GAGATGATGG GCTACCTGGT 
CCTCCAGGTC CAAAGGGACC CCAGGGGCTG CCTGGTTTCC CAGGTTTTCC CGGAGAAAGA 
GGAAAGCCTG GTGCAGAGGG ATGTCCTGGC ACAAAGGGAG AACCTGGAGA GAAGGGCATG 
TCTGGCCTTC CCGGAGACCG GGGACTGAGA GGGGCCAAAG GAGCCATAGG ACCTCCCGGA 
GATGAAGGAG AAATGGCTAT CATTTCCCAA AAGGGAACAC CTGGGGAACC TGGACCTCCT 
GGAGATGATG GATTCCCAGG AGAAAGAGGT GATAAAGGAA CTCCCGGGAT GCAAGGGAGA 
AGAGGAGAGC CGGGAAGATA CGGACCACCT GGATTTCAGA GAGGGGAACC TGGACTTCCA 
GGTGACCAGG GTGAGCCAGG TTCTCCAGGT CCCCCTGGAT TTTCAGGAAT TGATGGAGCA 
AGAGGACCTA AAGGAAACAA AGGTGACCCT GCCAGTCACT TTGGTCCACC TGGTCCAAAG 
GGTGAGCCAG GTAGCCCTGG ATGTCCAGGG CATTTTGGAG CATCTGGAGA GCAGGGCTTG 
CCTGGTATTC AAGGGCCCAG AGGATCACCT GGAAGGCCAG GGCCACCTGG CTCCTCTGGA 
CCACCAGGGT GCCCAGGTGA TCACGGGATG CCTGGGCTGA GGGGACAGCC AGGAGAAATG 
GGAGACCCTG GGCCAAGAGG CCTCCAGGGG GATCCAGGGA TACCAGGTCC TCCGGGAATA 
AAAGGTCCCT CCGGATCACC TGGCCTGAAC GGCTTGCATG GATTGAAAGG TCAGAAAGGA 
ACTAAAGGTG CTTCAGGTTT GCATGATGTG GGGCCACCTG GTCCAGTGGG AATACCTGGG 
CTAAAAGGGG AGAGAGGAGA CCCTGGGAGC CCAGGAATCT CTCCTCCAGG TCCTCGTGGA 
GAAAAAGGTC CCCCAGGACC CCCAGGGAGT TCAGGACCAC CTGGTCCTGC AGGTGCCACA 
GGAAGAGCTC CTAAGGACAT TCCTGACCCG GGTCCACCTG GAGATCAGGG ACCTCCTGGT 
CCTGATGGCC CAAGAGGAGC ACCTGGGCCT CCAGGCCTCC CTGGGAGTGT TGACCTTCTG 
AGAGGGGAGC CAGGTGACTG TGGTCTACCA GGGCCACCAG GTCCCCCTGG CCCACCAGGC 
CCTCCAGGAT ACAAAGGCTT TCCAGGATGT GATGGAAAAG ATGGCCAGAA AGGACCAATG 
GGATTCCCGG GACCGCAGGG ACCACATGGA TTTCCTGGGC CACCTGGAGA GAAGGGTTTA 
CCTGGACCTC CAGGGAGAAA AGGGCCCACT GGTCTTCCAG GTCCCAGAGG TGAACCGGGG 
CCACCTGCAG ATGTGGATGA CTGTCCCCGA ATCCCAGGCC TTCCTGGGGC GCCAGGCATG 
AGAGGACCAG AAGGAGCCAT GGGGCTCTCT GGAATGAGAG GCCCCCCAGG ACCAGGGTGC 
AAAGGAGAGC CTGGGCTGGA TGGCAGGAGG GGTGTGGATG GCGTCCCTGG GTCTCCTGGG 
CCTCCCGGAC GTAAAGGTGA CACAGGAGAA GACGGCTACC CTGGAGGACC AGGGCCTCCT 
GGTCCCATTG GGGATCCTGG GCCCAAAGGG TTTGGCCCTG GATACCTCGG TGGCTTCCTC 
CTGGTTCTCC ACAGTCAGAC GGACCAGGAG CCCACCTGCC CCCTGGGCAT GCCCAGGCTC 
TGGACTGGGT ATAGTCTGTT ATACCTGGAA GGGCAAGAGA AAGCTCACAA TCAAGACCTT 
GGTCTGGCAG GGTCTTGCCT TCCCGTGTTT AGCACGCTGC CCTTTGCCTA CTGCAACATC 
CACCAGGTGT GCCACTATGC CCAGAGAAAC GACAGATCCT ACTGGCTGGC CAGCGCTGCG 
CCCCTCCCCA TGATGCCACT CTCTGAAGAG GCGATCCGCC CCTATGTCAG CCGCTGTGCG 
GTATGCGAGG CCCCGGCCCA GGCGGTGGCG GTGCACAGCC AGGACCAGTC CATCCCCCCA 
TGTCCGCAGA CCTGGAGGAG CCTCTGGATC GGGTATTCAT TCCTGATGCA CACAGGAGCT 
GGGGACCAAG GAGGAGGGCA GGCCCTTATG TCACCTGGCA GCTGCCTGGA AGATTTCAGA 
GCAGCACCAT TCCTTGAATG CCAGGGCCGG CAGGGAACTT GCCACTTTTT TGCAAATAAG 
TATAGCTTCT GGCTCACAAC GGTGAAAGCA GACTTGCAGT TTTCCTCTGC TCCAGCACCA 
GACACCTTAA AAGAAAGCCA GGCCCAACGC CAGAAAATCA GCCGGTGCCA GGTCTGCGTG 
AAGTATAGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009442742.3
CDS	425..5416
Translation	MWSLHIVLMRCSFRLTKSLATDPWSLTLILFSVQYVYGSGKKYVGPCGGRDCSVCHCVPEKGSRGPPGPPGPQGPIGPLGAPGPIGLSGEKGMRGDRGPPGAAGDKGDKGSTGVPGFPGLDGIPGHPGPPGPRGKPGMSGHNGSRGDPGFPGGRGALGPGGPLGHPGEKGEKGNSVFILGAVKGIQGDRGDPGLPGLPGSWGAGGPAGPTGYPGEPGLVGPPGQPGRPGLKGNPGVGVKGQMGDPGEVGQQGSPGPTLLVEPPDFRLYKGEKGIKGIPGMVGLPGPPGRKGESDIGAKGEKGIPGFPGPRGDPGSYGSPGFPGLKGELGLVGDPGLFGLIGPKGDPGNRGHPGPPGVLVTPPLPLKGPPGDPGFPGRYGETGDVGPPGPPGLLGRPGEACAGMIGPPGPQGFPGLPGLPGEAGIPGRPDSAPGKPGKPGSPGLPGAPGLQGLPGSSVIYCSVGNPGPQGIKGKVGPPGGRGSKGEKGNEGLCACEPGPMGPPGPPGLPGRQGSKGDLGLPGWLGTKGDPGPPGAEGPPGLPGKHGASGPPGNKGAKGDMVVSRVKGHKGERGPDGPPGFPGQPGSHGRDGHAGEKGDPGPPGDHEDATPGGKGFPGPLGPPGKAGPLGPPGLGFPGPPGQRGHPGVPGHPGVRGPDGLKGQKGDTISCNVTYPGRHGPPGFDGPPGPKGFPGPQGAPGLSGSDGHKGRPGTPGTAEIPGPPGFRGDMGDPGFGGEKGSSPAGPPGPPGSPGVNGQKGIPGDPAFGHLGPPGKRGLSGVPGIKGPRGDPGCPGAEGPAGIPGFPGLKGPKGREGHAGFPGVPGPPGHSCERGAPGIPGQPGLPGYPGSPGAPGGKGQLGDVGPPGPAGMKGLPGLPGWPGAHGPPGLPGIPGPFGDDGLPGPPGPKGPQGLPGFPGFPGERGKPGAEGCPGTKGEPGEKGMSGLPGDRGLRGAKGAIGPPGDEGEMAIISQKGTPGEPGPPGDDGFPGERGDKGTPGMQGRRGEPGRYGPPGFQRGEPGLPGDQGEPGSPGPPGFSGIDGARGPKGNKGDPASHFGPPGPKGEPGSPGCPGHFGASGEQGLPGIQGPRGSPGRPGPPGSSGPPGCPGDHGMPGLRGQPGEMGDPGPRGLQGDPGIPGPPGIKGPSGSPGLNGLHGLKGQKGTKGASGLHDVGPPGPVGIPGLKGERGDPGSPGISPPGPRGEKGPPGPPGSSGPPGPAGATGRAPKDIPDPGPPGDQGPPGPDGPRGAPGPPGLPGSVDLLRGEPGDCGLPGPPGPPGPPGPPGYKGFPGCDGKDGQKGPMGFPGPQGPHGFPGPPGEKGLPGPPGRKGPTGLPGPRGEPGPPADVDDCPRIPGLPGAPGMRGPEGAMGLSGMRGPPGPGCKGEPGLDGRRGVDGVPGSPGPPGRKGDTGEDGYPGGPGPPGPIGDPGPKGFGPGYLGGFLLVLHSQTDQEPTCPLGMPRLWTGYSLLYLEGQEKAHNQDLGLAGSCLPVFSTLPFAYCNIHQVCHYAQRNDRSYWLASAAPLPMMPLSEEAIRPYVSRCAVCEAPAQAVAVHSQDQSIPPCPQTWRSLWIGYSFLMHTGAGDQGGGQALMSPGSCLEDFRAAPFLECQGRQGTCHFFANKYSFWLTTVKADLQFSSAPAPDTLKESQAQRQKISRCQVCVKYS

Target ORF information:

RefSeq Version	XM_009444467.3
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes collagen type IV alpha 4 chain (COL4A4), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009444467.3

ORF Insert Sequence:

ATGTGGTCTC TGCACATAGT ACTAATGAGG TGCTCCTTCA GATTGACCAA GTCCTTGGCC 
ACAGATCCCT GGTCACTTAC ACTCATTCTC TTTTCTGTAC AATATGTATA TGGGAGTGGA 
AAGAAATACG TTGGTCCTTG TGGAGGAAGA GATTGCTCTG TTTGCCACTG TGTTCCTGAA 
AAGGGGTCTC GGGGTCCACC AGGACCACCA GGGCCACAGG GTCCAATTGG ACCCCTGGGA 
GCCCCAGGAC CCATTGGGCT TTCAGGAGAG AAAGGAATGA GAGGGGACCG CGGCCCTCCT 
GGAGCAGCAG GGGACAAAGG AGATAAGGGT TCAACTGGTG TTCCTGGATT TCCAGGTTTA 
GATGGCATAC CTGGGCACCC AGGGCCTCCT GGACCCAGAG GCAAACCTGG TATGAGTGGC 
CACAATGGCT CAAGAGGTGA CCCAGGGTTT CCAGGAGGAA GAGGAGCTCT TGGCCCAGGA 
GGCCCCCTAG GCCATCCTGG GGAAAAGGGA GAAAAAGGAA ATTCAGTGTT CATTTTAGGT 
GCCGTTAAAG GTATTCAGGG AGACAGAGGG GACCCAGGAC TGCCTGGCTT ACCAGGATCT 
TGGGGGGCAG GAGGACCGGC GGGTCCCACA GGATATCCTG GAGAGCCAGG GTTAGTGGGA 
CCTCCGGGCC AACCAGGGCG TCCAGGTTTG AAGGGAAATC CCGGTGTGGG AGTAAAGGGG 
CAAATGGGAG ACCCGGGTGA GGTTGGTCAG CAAGGTTCTC CTGGACCCAC CCTGTTGGTG 
GAGCCACCTG ACTTTCGTCT CTATAAAGGA GAAAAGGGTA TAAAAGGAAT TCCTGGAATG 
GTTGGACTGC CAGGACCACC AGGACGCAAG GGAGAATCTG ATATTGGGGC AAAAGGAGAA 
AAAGGTATTC CTGGATTTCC AGGGCCTCGG GGGGATCCTG GTTCCTATGG ATCTCCAGGT 
TTTCCAGGAT TAAAGGGAGA ACTAGGACTG GTTGGAGATC CTGGGCTATT TGGATTAATT 
GGCCCAAAGG GGGATCCTGG AAATCGAGGG CACCCAGGAC CACCAGGTGT TTTGGTGACT 
CCACCTCTTC CACTCAAAGG CCCACCAGGG GACCCAGGGT TCCCTGGCCG CTATGGAGAA 
ACAGGGGATG TTGGACCACC TGGTCCCCCA GGTCTCTTGG GCAGACCAGG GGAAGCCTGT 
GCAGGCATGA TAGGACCCCC TGGGCCACAA GGATTTCCTG GTCTTCCTGG GCTTCCAGGA 
GAAGCTGGTA TTCCTGGGAG ACCTGATTCT GCTCCAGGAA AACCAGGGAA GCCAGGATCA 
CCTGGCTTGC CTGGAGCACC AGGCCTGCAG GGCCTCCCAG GATCAAGTGT GATATACTGT 
AGTGTTGGGA ATCCTGGACC ACAAGGAATA AAAGGCAAAG TGGGTCCCCC AGGAGGAAGA 
GGCTCAAAAG GAGAAAAAGG AAATGAAGGA CTCTGTGCCT GTGAGCCTGG ACCCATGGGC 
CCCCCTGGCC CTCCAGGACT TCCTGGGAGG CAGGGGAGTA AGGGAGACTT GGGGCTCCCT 
GGCTGGCTTG GAACAAAAGG TGACCCAGGA CCTCCTGGTG CTGAAGGACC TCCAGGGCTA 
CCAGGAAAGC ATGGTGCCTC TGGACCACCT GGCAACAAAG GGGCGAAGGG TGACATGGTT 
GTATCAAGAG TTAAAGGGCA CAAAGGAGAA AGAGGTCCTG ATGGGCCCCC AGGATTTCCA 
GGGCAGCCAG GATCACATGG TCGGGATGGA CATGCTGGAG AAAAAGGGGA TCCAGGACCC 
CCAGGGGATC ATGAAGATGC GACCCCAGGT GGTAAAGGAT TTCCTGGACC TCTGGGCCCC 
CCGGGCAAAG CAGGACCTTT GGGGCCCCCA GGACTGGGAT TTCCTGGTCC ACCAGGACAG 
CGAGGCCACC CAGGAGTTCC AGGCCACCCA GGTGTGAGGG GCCCTGATGG CTTGAAGGGT 
CAGAAAGGTG ACACAATTTC TTGCAACGTA ACCTACCCTG GGAGGCACGG CCCTCCAGGT 
TTTGATGGAC CTCCAGGTCC GAAGGGATTT CCAGGTCCCC AAGGTGCCCC TGGGCTGAGT 
GGTTCAGATG GGCATAAAGG CAGACCTGGC ACACCAGGAA CAGCGGAAAT ACCAGGTCCA 
CCTGGTTTTC GTGGTGACAT GGGAGATCCG GGTTTTGGAG GTGAAAAGGG GTCCTCCCCT 
GCTGGGCCCC CAGGCCCTCC CGGCTCACCA GGAGTGAATG GTCAGAAAGG AATCCCGGGA 
GACCCTGCAT TTGGTCACCT GGGACCCCCG GGAAAGAGGG GTCTTTCAGG AGTGCCAGGG 
ATAAAAGGAC CCAGAGGTGA TCCGGGATGT CCAGGGGCTG AAGGGCCAGC TGGCATTCCT 
GGATTCCCAG GTCTCAAAGG TCCCAAAGGC AGAGAGGGAC ATGCTGGGTT TCCAGGTGTC 
CCAGGTCCAC CTGGCCATTC CTGTGAAAGA GGTGCTCCAG GGATACCAGG GCAACCGGGA 
CTCCCTGGGT ATCCAGGTAG CCCAGGTGCT CCAGGTGGGA AAGGACAGCT GGGAGATGTG 
GGGCCTCCCG GGCCAGCTGG AATGAAAGGC CTCCCTGGAC TCCCAGGATG GCCTGGGGCA 
CATGGTCCCC CAGGCCTCCC AGGAATCCCA GGTCCCTTTG GAGATGATGG GCTACCTGGT 
CCTCCAGGTC CAAAGGGACC CCAGGGGCTG CCTGGTTTCC CAGGTTTTCC CGGAGAAAGA 
GGAAAGCCTG GTGCAGAGGG ATGTCCTGGC ACAAAGGGAG AACCTGGAGA GAAGGGCATG 
TCTGGCCTTC CCGGAGACCG GGGACTGAGA GGGGCCAAAG GAGCCATAGG ACCTCCCGGA 
GATGAAGGAG AAATGGCTAT CATTTCCCAA AAGGGAACAC CTGGGGAACC TGGACCTCCT 
GGAGATGATG GATTCCCAGG AGAAAGAGGT GATAAAGGAA CTCCCGGGAT GCAAGGGAGA 
AGAGGAGAGC CGGGAAGATA CGGACCACCT GGATTTCAGA GAGGGGAACC TGGACTTCCA 
GGTGACCAGG GTGAGCCAGG TTCTCCAGGT CCCCCTGGAT TTTCAGGAAT TGATGGAGCA 
AGAGGACCTA AAGGAAACAA AGGTGACCCT GCCAGTCACT TTGGTCCACC TGGTCCAAAG 
GGTGAGCCAG GTAGCCCTGG ATGTCCAGGG CATTTTGGAG CATCTGGAGA GCAGGGCTTG 
CCTGGTATTC AAGGGCCCAG AGGATCACCT GGAAGGCCAG GGCCACCTGG CTCCTCTGGA 
CCACCAGGGT GCCCAGGTGA TCACGGGATG CCTGGGCTGA GGGGACAGCC AGGAGAAATG 
GGAGACCCTG GGCCAAGAGG CCTCCAGGGG GATCCAGGGA TACCAGGTCC TCCGGGAATA 
AAAGGTCCCT CCGGATCACC TGGCCTGAAC GGCTTGCATG GATTGAAAGG TCAGAAAGGA 
ACTAAAGGTG CTTCAGGTTT GCATGATGTG GGGCCACCTG GTCCAGTGGG AATACCTGGG 
CTAAAAGGGG AGAGAGGAGA CCCTGGGAGC CCAGGAATCT CTCCTCCAGG TCCTCGTGGA 
GAAAAAGGTC CCCCAGGACC CCCAGGGAGT TCAGGACCAC CTGGTCCTGC AGGTGCCACA 
GGAAGAGCTC CTAAGGACAT TCCTGACCCG GGTCCACCTG GAGATCAGGG ACCTCCTGGT 
CCTGATGGCC CAAGAGGAGC ACCTGGGCCT CCAGGCCTCC CTGGGAGTGT TGACCTTCTG 
AGAGGGGAGC CAGGTGACTG TGGTCTACCA GGGCCACCAG GTCCCCCTGG CCCACCAGGC 
CCTCCAGGAT ACAAAGGCTT TCCAGGATGT GATGGAAAAG ATGGCCAGAA AGGACCAATG 
GGATTCCCGG GACCGCAGGG ACCACATGGA TTTCCTGGGC CACCTGGAGA GAAGGGTTTA 
CCTGGACCTC CAGGGAGAAA AGGGCCCACT GGTCTTCCAG GTCCCAGAGG TGAACCGGGG 
CCACCTGCAG ATGTGGATGA CTGTCCCCGA ATCCCAGGCC TTCCTGGGGC GCCAGGCATG 
AGAGGACCAG AAGGAGCCAT GGGGCTCTCT GGAATGAGAG GCCCCCCAGG ACCAGGGTGC 
AAAGGAGAGC CTGGGCTGGA TGGCAGGAGG GGTGTGGATG GCGTCCCTGG GTCTCCTGGG 
CCTCCCGGAC GTAAAGGTGA CACAGGAGAA GACGGCTACC CTGGAGGACC AGGGCCTCCT 
GGTCCCATTG GGGATCCTGG GCCCAAAGGG TTTGGCCCTG GATACCTCGG TGGCTTCCTC 
CTGGTTCTCC ACAGTCAGAC GGACCAGGAG CCCACCTGCC CCCTGGGCAT GCCCAGGCTC 
TGGACTGGGT ATAGTCTGTT ATACCTGGAA GGGCAAGAGA AAGCTCACAA TCAAGACCTT 
GGTCTGGCAG GGTCTTGCCT TCCCGTGTTT AGCACGCTGC CCTTTGCCTA CTGCAACATC 
CACCAGGTGT GCCACTATGC CCAGAGAAAC GACAGATCCT ACTGGCTGGC CAGCGCTGCG 
CCCCTCCCCA TGATGCCACT CTCTGAAGAG GCGATCCGCC CCTATGTCAG CCGCTGTGCG 
GTATGCGAGG CCCCGGCCCA GGCGGTGGCG GTGCACAGCC AGGACCAGTC CATCCCCCCA 
TGTCCGCAGA CCTGGAGGAG CCTCTGGATC GGGTATTCAT TCCTGATGCA CACAGGAGCT 
GGGGACCAAG GAGGAGGGCA GGCCCTTATG TCACCTGGCA GCTGCCTGGA AGATTTCAGA 
GCAGCACCAT TCCTTGAATG CCAGGGCCGG CAGGGAACTT GCCACTTTTT TGCAAATAAG 
TATAGCTTCT GGCTCACAAC GGTGAAAGCA GACTTGCAGT TTTCCTCTGC TCCAGCACCA 
GACACCTTAA AAGAAAGCCA GGCCCAACGC CAGAAAATCA GCCGGTGCCA GGTCTGCGTG 
AAGTATAGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL4A4 ( XM_016950592.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016806081.2 Pan troglodytes collagen type IV alpha 4 chain (COL4A4), transcript variant X7, mRNA.

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CloneID	OPc104272
Clone ID Related Accession (Same CDS sequence)	XM_016950592.2
Accession Version	XM_016950592.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4965bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	collagen alpha-4(IV) chain isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036881.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_016950592.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGTCTC TGCACATAGT ACTAATGAGG TGCTCCTTCA GATTGACCAA GTCCTTGGCC 
ACAGATCCCT GGTCACTTAC ACTCATTCTC TTTTCTGTAC AATATGTATA TGGGAGTGGA 
AAGAAATACG TTGGTCCTTG TGGAGGAAGA GATTGCTCTG TTTGCCACTG TGTTCCTGAA 
AAGGGGTCTC GGGGTCCACC AGGACCACCA GGGCCACAGG GTCCAATTGG ACCCCTGGGA 
GCCCCAGGAC CCATTGGGCT TTCAGGAGAG AAAGGAATGA GAGGGGACCG CGGCCCTCCT 
GGAGCAGCAG GGGACAAAGG AGATAAGGGT TCAACTGGTG TTCCTGGATT TCCAGGTTTA 
GATGGCATAC CTGGGCACCC AGGGCCTCCT GGACCCAGAG GCAAACCTGG TATGAGTGGC 
CACAATGGCT CAAGAGGTGA CCCAGGGTTT CCAGGAGGAA GAGGAGCTCT TGGCCCAGGA 
GGCCCCCTAG GCCATCCTGG GGAAAAGGGA GAAAAAGGAA ATTCAGTGTT CATTTTAGGT 
GCCGTTAAAG GTATTCAGGG AGACAGAGGG GACCCAGGAC TGCCTGGCTT ACCAGGATCT 
TGGGGGGCAG GAGGACCGGC GGGTCCCACA GGATATCCTG GAGAGCCAGG GTTAGTGGGA 
CCTCCGGGCC AACCAGGGCG TCCAGGTTTG AAGGGAAATC CCGGTGTGGG AGTAAAGGGG 
CAAATGGGAG ACCCGGGTGA GGTTGGTCAG CAAGGTTCTC CTGGACCCAC CCTGTTGGTG 
GAGCCACCTG ACTTTCGTCT CTATAAAGGA GAAAAGGGTA TAAAAGGAAT TCCTGGAATG 
GTTGGACTGC CAGGACCACC AGGACGCAAG GGAGAATCTG ATATTGGGGC AAAAGGAGAA 
AAAGGTATTC CTGGATTTCC AGGGCCTCGG GGGGATCCTG GTTCCTATGG ATCTCCAGGT 
TTTCCAGGAT TAAAGGGAGA ACTAGGACTG GTTGGAGATC CTGGGCTATT TGGATTAATT 
GGCCCAAAGG GGGATCCTGG AAATCGAGGG CACCCAGGAC CACCAGGTGT TTTGGTGACT 
CCACCTCTTC CACTCAAAGG CCCACCAGGG GACCCAGGGT TCCCTGGCCG CTATGGAGAA 
ACAGGGGATG TTGGACCACC TGGTCCCCCA GGTCTCTTGG GCAGACCAGG GGAAGCCTGT 
GCAGGCATGA TAGGACCCCC TGGGCCACAA GGATTTCCTG GTCTTCCTGG GCTTCCAGGA 
GAAGCTGGTA TTCCTGGGAG ACCTGATTCT GCTCCAGGAA AACCAGGGAA GCCAGGATCA 
CCTGGCTTGC CTGGAGCACC AGGCCTGCAG GGCCTCCCAG GATCAAGTGT GATATACTGT 
AGTGTTGGGA ATCCTGGACC ACAAGGAATA AAAGGCAAAG TGGGTCCCCC AGGAGGAAGA 
GGCTCAAAAG GAGAAAAAGG AAATGAAGGA CTCTGTGCCT GTGAGCCTGG ACCCATGGGC 
CCCCCTGGCC CTCCAGGACT TCCTGGGAGG CAGGGGAGTA AGGGAGACTT GGGGCTCCCT 
GGCTGGCTTG GAACAAAAGG TGACCCAGGA CCTCCTGGTG CTGAAGGACC TCCAGGGCTA 
CCAGGAAAGC ATGGTGCCTC TGGACCACCT GGCAACAAAG GGGCGAAGGG TGACATGGTT 
GTATCAAGAG TTAAAGGGCA CAAAGGAGAA AGAGGTCCTG ATGGGCCCCC AGGATTTCCA 
GGGCAGCCAG GATCACATGG TCGGGATGGA CATGCTGGAG AAAAAGGGGA TCCAGGACCC 
CCAGGGGATC ATGAAGATGC GACCCCAGGT GGTAAAGGAT TTCCTGGACC TCTGGGCCCC 
CCGGGCAAAG CAGGACCTTT GGGGCCCCCA GGACTGGGAT TTCCTGGTCC ACCAGGACAG 
CGAGGCCACC CAGGAGTTCC AGGCCACCCA GGTGTGAGGG GCCCTGATGG CTTGAAGGGT 
CAGAAAGGTG ACACAATTTC TTGCAACGTA ACCTACCCTG GGAGGCACGG CCCTCCAGGT 
TTTGATGGAC CTCCAGGTTT TCGTGGTGAC ATGGGAGATC CGGGTTTTGG AGGTGAAAAG 
GGGTCCTCCC CTGCTGGGCC CCCAGGCCCT CCCGGCTCAC CAGGAGTGAA TGGTCAGAAA 
GGAATCCCGG GAGACCCTGC ATTTGGTCAC CTGGGACCCC CGGGAAAGAG GGGTCTTTCA 
GGAGTGCCAG GGATAAAAGG ACCCAGAGGT GATCCGGGAT GTCCAGGGGC TGAAGGGCCA 
GCTGGCATTC CTGGATTCCC AGGTCTCAAA GGTCCCAAAG GCAGAGAGGG ACATGCTGGG 
TTTCCAGGTG TCCCAGGTCC ACCTGGCCAT TCCTGTGAAA GAGGTGCTCC AGGGATACCA 
GGGCAACCGG GACTCCCTGG GTATCCAGGT AGCCCAGGTG CTCCAGGTGG GAAAGGACAG 
CTGGGAGATG TGGGGCCTCC CGGGCCAGCT GGAATGAAAG GCCTCCCTGG ACTCCCAGGA 
TGGCCTGGGG CACATGGTCC CCCAGGCCTC CCAGGAATCC CAGGTCCCTT TGGAGATGAT 
GGGCTACCTG GTCCTCCAGG TCCAAAGGGA CCCCAGGGGC TGCCTGGTTT CCCAGGTTTT 
CCCGGAGAAA GAGGAAAGCC TGGTGCAGAG GGATGTCCTG GCACAAAGGG AGAACCTGGA 
GAGAAGGGCA TGTCTGGCCT TCCCGGAGAC CGGGGACTGA GAGGGGCCAA AGGAGCCATA 
GGACCTCCCG GAGATGAAGG AGAAATGGCT ATCATTTCCC AAAAGGGAAC ACCTGGGGAA 
CCTGGACCTC CTGGAGATGA TGGATTCCCA GGAGAAAGAG GTGATAAAGG AACTCCCGGG 
ATGCAAGGGA GAAGAGGAGA GCCGGGAAGA TACGGACCAC CTGGATTTCA GAGAGGGGAA 
CCTGGTGAGA AAGGTCAGCC AGGGCATCCT GGACCCCCAG GCCCTCCAGG CTCAACTGGT 
CTAAGAGGGT TCATTGGTTT TCCAGGACTT CCAGGTGACC AGGGTGAGCC AGGTTCTCCA 
GGTCCCCCTG GATTTTCAGG AATTGATGGA GCAAGAGGAC CTAAAGGAAA CAAAGGTGAC 
CCTGCCAGTC ACTTTGGTCC ACCTGGTCCA AAGGGTGAGC CAGGTAGCCC TGGATGTCCA 
GGGCATTTTG GAGCATCTGG AGAGCAGGGC TTGCCTGGTA TTCAAGGGCC CAGAGGATCA 
CCTGGAAGGC CAGGGCCACC TGGCTCCTCT GGACCACCAG GGTGCCCAGG TGATCACGGG 
ATGCCTGGGC TGAGGGGACA GCCAGGAGAA ATGGGAGACC CTGGGCCAAG AGGCCTCCAG 
GGGGATCCAG GGATACCAGG TCCTCCGGGA ATAAAAGGTC CCTCCGGATC ACCTGGCCTG 
AACGGCTTGC ATGGATTGAA AGGTCAGAAA GGAACTAAAG GTGCTTCAGG TTTGCATGAT 
GTGGGGCCAC CTGGTCCAGT GGGAATACCT GGGCTAAAAG GGGAGAGAGG AGACCCTGGG 
AGCCCAGGAA TCTCTCCTCC AGGTCCTCGT GGAGAAAAAG GTCCCCCAGG ACCCCCAGGG 
AGTTCAGGAC CACCTGGTCC TGCAGGTGCC ACAGGAAGAG CTCCTAAGGA CATTCCTGAC 
CCGGGTCCAC CTGGAGATCA GGGACCTCCT GGTCCTGATG GCCCAAGAGG AGCACCTGGG 
CCTCCAGGCC TCCCTGGGAG TGTTGACCTT CTGAGAGGGG AGCCAGGTGA CTGTGGTCTA 
CCAGGGCCAC CAGGTCCCCC TGGCCCACCA GGCCCTCCAG GATACAAAGG CTTTCCAGGA 
TGTGATGGAA AAGATGGCCA GAAAGGACCA ATGGGATTCC CGGGACCGCA GGGACCACAT 
GGATTTCCTG GGCCACCTGG AGAGAAGGGT TTACCTGGAC CTCCAGGGAG AAAAGGGCCC 
ACTGGTCTTC CAGGTCCCAG AGGTGAACCG GGGCCACCTG CAGATGTGGA TGACTGTCCC 
CGAATCCCAG GCCTTCCTGG GGCGCCAGGC ATGAGAGGAC CAGAAGGAGC CATGGGGCTC 
TCTGGAATGA GAGGCCCCCC AGGACCAGGG TGCAAAGGAG AGCCTGGGCT GGATGGCAGG 
AGGGGTGTGG ATGGCGTCCC TGGGTCTCCT GGGCCTCCCG GACGTAAAGG TGACACAGGA 
GAAGACGGCT ACCCTGGAGG ACCAGGGCCT CCTGGTCCCA TTGGGGATCC TGGGCCCAAA 
GGGTTTGGCC CTGGATACCT CGGTGGCTTC CTCCTGGTTC TCCACAGTCA GACGGACCAG 
GAGCCCACCT GCCCCCTGGG CATGCCCAGG CTCTGGACTG GGTATAGTCT GTTATACCTG 
GAAGGGCAAG AGAAAGCTCA CAATCAAGAC CTTGGTCTGG CAGGGTCTTG CCTTCCCGTG 
TTTAGCACGC TGCCCTTTGC CTACTGCAAC ATCCACCAGG TGTGCCACTA TGCCCAGAGA 
AACGACAGAT CCTACTGGCT GGCCAGCGCT GCGCCCCTCC CCATGATGCC ACTCTCTGAA 
GAGGCGATCC GCCCCTATGT CAGCCGCTGT GCGGTATGCG AGGCCCCGGC CCAGGCGGTG 
GCGGTGCACA GCCAGGACCA GTCCATCCCC CCATGTCCGC AGACCTGGAG GAGCCTCTGG 
ATCGGGTATT CATTCCTGAT GCACACAGGA GCTGGGGACC AAGGAGGAGG GCAGGCCCTT 
ATGTCACCTG GCAGCTGCCT GGAAGATTTC AGAGCAGCAC CATTCCTTGA ATGCCAGGGC 
CGGCAGGGAA CTTGCCACTT TTTTGCAAAT AAGTATAGCT TCTGGCTCAC AACGGTGAAA 
GCAGACTTGC AGTTTTCCTC TGCTCCAGCA CCAGACACCT TAAAAGAAAG CCAGGCCCAA 
CGCCAGAAAA TCAGCCGGTG CCAGGTCTGC GTGAAGTATA GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016806081.2
CDS	425..5389
Translation	MWSLHIVLMRCSFRLTKSLATDPWSLTLILFSVQYVYGSGKKYVGPCGGRDCSVCHCVPEKGSRGPPGPPGPQGPIGPLGAPGPIGLSGEKGMRGDRGPPGAAGDKGDKGSTGVPGFPGLDGIPGHPGPPGPRGKPGMSGHNGSRGDPGFPGGRGALGPGGPLGHPGEKGEKGNSVFILGAVKGIQGDRGDPGLPGLPGSWGAGGPAGPTGYPGEPGLVGPPGQPGRPGLKGNPGVGVKGQMGDPGEVGQQGSPGPTLLVEPPDFRLYKGEKGIKGIPGMVGLPGPPGRKGESDIGAKGEKGIPGFPGPRGDPGSYGSPGFPGLKGELGLVGDPGLFGLIGPKGDPGNRGHPGPPGVLVTPPLPLKGPPGDPGFPGRYGETGDVGPPGPPGLLGRPGEACAGMIGPPGPQGFPGLPGLPGEAGIPGRPDSAPGKPGKPGSPGLPGAPGLQGLPGSSVIYCSVGNPGPQGIKGKVGPPGGRGSKGEKGNEGLCACEPGPMGPPGPPGLPGRQGSKGDLGLPGWLGTKGDPGPPGAEGPPGLPGKHGASGPPGNKGAKGDMVVSRVKGHKGERGPDGPPGFPGQPGSHGRDGHAGEKGDPGPPGDHEDATPGGKGFPGPLGPPGKAGPLGPPGLGFPGPPGQRGHPGVPGHPGVRGPDGLKGQKGDTISCNVTYPGRHGPPGFDGPPGFRGDMGDPGFGGEKGSSPAGPPGPPGSPGVNGQKGIPGDPAFGHLGPPGKRGLSGVPGIKGPRGDPGCPGAEGPAGIPGFPGLKGPKGREGHAGFPGVPGPPGHSCERGAPGIPGQPGLPGYPGSPGAPGGKGQLGDVGPPGPAGMKGLPGLPGWPGAHGPPGLPGIPGPFGDDGLPGPPGPKGPQGLPGFPGFPGERGKPGAEGCPGTKGEPGEKGMSGLPGDRGLRGAKGAIGPPGDEGEMAIISQKGTPGEPGPPGDDGFPGERGDKGTPGMQGRRGEPGRYGPPGFQRGEPGEKGQPGHPGPPGPPGSTGLRGFIGFPGLPGDQGEPGSPGPPGFSGIDGARGPKGNKGDPASHFGPPGPKGEPGSPGCPGHFGASGEQGLPGIQGPRGSPGRPGPPGSSGPPGCPGDHGMPGLRGQPGEMGDPGPRGLQGDPGIPGPPGIKGPSGSPGLNGLHGLKGQKGTKGASGLHDVGPPGPVGIPGLKGERGDPGSPGISPPGPRGEKGPPGPPGSSGPPGPAGATGRAPKDIPDPGPPGDQGPPGPDGPRGAPGPPGLPGSVDLLRGEPGDCGLPGPPGPPGPPGPPGYKGFPGCDGKDGQKGPMGFPGPQGPHGFPGPPGEKGLPGPPGRKGPTGLPGPRGEPGPPADVDDCPRIPGLPGAPGMRGPEGAMGLSGMRGPPGPGCKGEPGLDGRRGVDGVPGSPGPPGRKGDTGEDGYPGGPGPPGPIGDPGPKGFGPGYLGGFLLVLHSQTDQEPTCPLGMPRLWTGYSLLYLEGQEKAHNQDLGLAGSCLPVFSTLPFAYCNIHQVCHYAQRNDRSYWLASAAPLPMMPLSEEAIRPYVSRCAVCEAPAQAVAVHSQDQSIPPCPQTWRSLWIGYSFLMHTGAGDQGGGQALMSPGSCLEDFRAAPFLECQGRQGTCHFFANKYSFWLTTVKADLQFSSAPAPDTLKESQAQRQKISRCQVCVKYS

Target ORF information:

RefSeq Version	XM_016950592.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes collagen type IV alpha 4 chain (COL4A4), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016950592.2

ORF Insert Sequence:

ATGTGGTCTC TGCACATAGT ACTAATGAGG TGCTCCTTCA GATTGACCAA GTCCTTGGCC 
ACAGATCCCT GGTCACTTAC ACTCATTCTC TTTTCTGTAC AATATGTATA TGGGAGTGGA 
AAGAAATACG TTGGTCCTTG TGGAGGAAGA GATTGCTCTG TTTGCCACTG TGTTCCTGAA 
AAGGGGTCTC GGGGTCCACC AGGACCACCA GGGCCACAGG GTCCAATTGG ACCCCTGGGA 
GCCCCAGGAC CCATTGGGCT TTCAGGAGAG AAAGGAATGA GAGGGGACCG CGGCCCTCCT 
GGAGCAGCAG GGGACAAAGG AGATAAGGGT TCAACTGGTG TTCCTGGATT TCCAGGTTTA 
GATGGCATAC CTGGGCACCC AGGGCCTCCT GGACCCAGAG GCAAACCTGG TATGAGTGGC 
CACAATGGCT CAAGAGGTGA CCCAGGGTTT CCAGGAGGAA GAGGAGCTCT TGGCCCAGGA 
GGCCCCCTAG GCCATCCTGG GGAAAAGGGA GAAAAAGGAA ATTCAGTGTT CATTTTAGGT 
GCCGTTAAAG GTATTCAGGG AGACAGAGGG GACCCAGGAC TGCCTGGCTT ACCAGGATCT 
TGGGGGGCAG GAGGACCGGC GGGTCCCACA GGATATCCTG GAGAGCCAGG GTTAGTGGGA 
CCTCCGGGCC AACCAGGGCG TCCAGGTTTG AAGGGAAATC CCGGTGTGGG AGTAAAGGGG 
CAAATGGGAG ACCCGGGTGA GGTTGGTCAG CAAGGTTCTC CTGGACCCAC CCTGTTGGTG 
GAGCCACCTG ACTTTCGTCT CTATAAAGGA GAAAAGGGTA TAAAAGGAAT TCCTGGAATG 
GTTGGACTGC CAGGACCACC AGGACGCAAG GGAGAATCTG ATATTGGGGC AAAAGGAGAA 
AAAGGTATTC CTGGATTTCC AGGGCCTCGG GGGGATCCTG GTTCCTATGG ATCTCCAGGT 
TTTCCAGGAT TAAAGGGAGA ACTAGGACTG GTTGGAGATC CTGGGCTATT TGGATTAATT 
GGCCCAAAGG GGGATCCTGG AAATCGAGGG CACCCAGGAC CACCAGGTGT TTTGGTGACT 
CCACCTCTTC CACTCAAAGG CCCACCAGGG GACCCAGGGT TCCCTGGCCG CTATGGAGAA 
ACAGGGGATG TTGGACCACC TGGTCCCCCA GGTCTCTTGG GCAGACCAGG GGAAGCCTGT 
GCAGGCATGA TAGGACCCCC TGGGCCACAA GGATTTCCTG GTCTTCCTGG GCTTCCAGGA 
GAAGCTGGTA TTCCTGGGAG ACCTGATTCT GCTCCAGGAA AACCAGGGAA GCCAGGATCA 
CCTGGCTTGC CTGGAGCACC AGGCCTGCAG GGCCTCCCAG GATCAAGTGT GATATACTGT 
AGTGTTGGGA ATCCTGGACC ACAAGGAATA AAAGGCAAAG TGGGTCCCCC AGGAGGAAGA 
GGCTCAAAAG GAGAAAAAGG AAATGAAGGA CTCTGTGCCT GTGAGCCTGG ACCCATGGGC 
CCCCCTGGCC CTCCAGGACT TCCTGGGAGG CAGGGGAGTA AGGGAGACTT GGGGCTCCCT 
GGCTGGCTTG GAACAAAAGG TGACCCAGGA CCTCCTGGTG CTGAAGGACC TCCAGGGCTA 
CCAGGAAAGC ATGGTGCCTC TGGACCACCT GGCAACAAAG GGGCGAAGGG TGACATGGTT 
GTATCAAGAG TTAAAGGGCA CAAAGGAGAA AGAGGTCCTG ATGGGCCCCC AGGATTTCCA 
GGGCAGCCAG GATCACATGG TCGGGATGGA CATGCTGGAG AAAAAGGGGA TCCAGGACCC 
CCAGGGGATC ATGAAGATGC GACCCCAGGT GGTAAAGGAT TTCCTGGACC TCTGGGCCCC 
CCGGGCAAAG CAGGACCTTT GGGGCCCCCA GGACTGGGAT TTCCTGGTCC ACCAGGACAG 
CGAGGCCACC CAGGAGTTCC AGGCCACCCA GGTGTGAGGG GCCCTGATGG CTTGAAGGGT 
CAGAAAGGTG ACACAATTTC TTGCAACGTA ACCTACCCTG GGAGGCACGG CCCTCCAGGT 
TTTGATGGAC CTCCAGGTTT TCGTGGTGAC ATGGGAGATC CGGGTTTTGG AGGTGAAAAG 
GGGTCCTCCC CTGCTGGGCC CCCAGGCCCT CCCGGCTCAC CAGGAGTGAA TGGTCAGAAA 
GGAATCCCGG GAGACCCTGC ATTTGGTCAC CTGGGACCCC CGGGAAAGAG GGGTCTTTCA 
GGAGTGCCAG GGATAAAAGG ACCCAGAGGT GATCCGGGAT GTCCAGGGGC TGAAGGGCCA 
GCTGGCATTC CTGGATTCCC AGGTCTCAAA GGTCCCAAAG GCAGAGAGGG ACATGCTGGG 
TTTCCAGGTG TCCCAGGTCC ACCTGGCCAT TCCTGTGAAA GAGGTGCTCC AGGGATACCA 
GGGCAACCGG GACTCCCTGG GTATCCAGGT AGCCCAGGTG CTCCAGGTGG GAAAGGACAG 
CTGGGAGATG TGGGGCCTCC CGGGCCAGCT GGAATGAAAG GCCTCCCTGG ACTCCCAGGA 
TGGCCTGGGG CACATGGTCC CCCAGGCCTC CCAGGAATCC CAGGTCCCTT TGGAGATGAT 
GGGCTACCTG GTCCTCCAGG TCCAAAGGGA CCCCAGGGGC TGCCTGGTTT CCCAGGTTTT 
CCCGGAGAAA GAGGAAAGCC TGGTGCAGAG GGATGTCCTG GCACAAAGGG AGAACCTGGA 
GAGAAGGGCA TGTCTGGCCT TCCCGGAGAC CGGGGACTGA GAGGGGCCAA AGGAGCCATA 
GGACCTCCCG GAGATGAAGG AGAAATGGCT ATCATTTCCC AAAAGGGAAC ACCTGGGGAA 
CCTGGACCTC CTGGAGATGA TGGATTCCCA GGAGAAAGAG GTGATAAAGG AACTCCCGGG 
ATGCAAGGGA GAAGAGGAGA GCCGGGAAGA TACGGACCAC CTGGATTTCA GAGAGGGGAA 
CCTGGTGAGA AAGGTCAGCC AGGGCATCCT GGACCCCCAG GCCCTCCAGG CTCAACTGGT 
CTAAGAGGGT TCATTGGTTT TCCAGGACTT CCAGGTGACC AGGGTGAGCC AGGTTCTCCA 
GGTCCCCCTG GATTTTCAGG AATTGATGGA GCAAGAGGAC CTAAAGGAAA CAAAGGTGAC 
CCTGCCAGTC ACTTTGGTCC ACCTGGTCCA AAGGGTGAGC CAGGTAGCCC TGGATGTCCA 
GGGCATTTTG GAGCATCTGG AGAGCAGGGC TTGCCTGGTA TTCAAGGGCC CAGAGGATCA 
CCTGGAAGGC CAGGGCCACC TGGCTCCTCT GGACCACCAG GGTGCCCAGG TGATCACGGG 
ATGCCTGGGC TGAGGGGACA GCCAGGAGAA ATGGGAGACC CTGGGCCAAG AGGCCTCCAG 
GGGGATCCAG GGATACCAGG TCCTCCGGGA ATAAAAGGTC CCTCCGGATC ACCTGGCCTG 
AACGGCTTGC ATGGATTGAA AGGTCAGAAA GGAACTAAAG GTGCTTCAGG TTTGCATGAT 
GTGGGGCCAC CTGGTCCAGT GGGAATACCT GGGCTAAAAG GGGAGAGAGG AGACCCTGGG 
AGCCCAGGAA TCTCTCCTCC AGGTCCTCGT GGAGAAAAAG GTCCCCCAGG ACCCCCAGGG 
AGTTCAGGAC CACCTGGTCC TGCAGGTGCC ACAGGAAGAG CTCCTAAGGA CATTCCTGAC 
CCGGGTCCAC CTGGAGATCA GGGACCTCCT GGTCCTGATG GCCCAAGAGG AGCACCTGGG 
CCTCCAGGCC TCCCTGGGAG TGTTGACCTT CTGAGAGGGG AGCCAGGTGA CTGTGGTCTA 
CCAGGGCCAC CAGGTCCCCC TGGCCCACCA GGCCCTCCAG GATACAAAGG CTTTCCAGGA 
TGTGATGGAA AAGATGGCCA GAAAGGACCA ATGGGATTCC CGGGACCGCA GGGACCACAT 
GGATTTCCTG GGCCACCTGG AGAGAAGGGT TTACCTGGAC CTCCAGGGAG AAAAGGGCCC 
ACTGGTCTTC CAGGTCCCAG AGGTGAACCG GGGCCACCTG CAGATGTGGA TGACTGTCCC 
CGAATCCCAG GCCTTCCTGG GGCGCCAGGC ATGAGAGGAC CAGAAGGAGC CATGGGGCTC 
TCTGGAATGA GAGGCCCCCC AGGACCAGGG TGCAAAGGAG AGCCTGGGCT GGATGGCAGG 
AGGGGTGTGG ATGGCGTCCC TGGGTCTCCT GGGCCTCCCG GACGTAAAGG TGACACAGGA 
GAAGACGGCT ACCCTGGAGG ACCAGGGCCT CCTGGTCCCA TTGGGGATCC TGGGCCCAAA 
GGGTTTGGCC CTGGATACCT CGGTGGCTTC CTCCTGGTTC TCCACAGTCA GACGGACCAG 
GAGCCCACCT GCCCCCTGGG CATGCCCAGG CTCTGGACTG GGTATAGTCT GTTATACCTG 
GAAGGGCAAG AGAAAGCTCA CAATCAAGAC CTTGGTCTGG CAGGGTCTTG CCTTCCCGTG 
TTTAGCACGC TGCCCTTTGC CTACTGCAAC ATCCACCAGG TGTGCCACTA TGCCCAGAGA 
AACGACAGAT CCTACTGGCT GGCCAGCGCT GCGCCCCTCC CCATGATGCC ACTCTCTGAA 
GAGGCGATCC GCCCCTATGT CAGCCGCTGT GCGGTATGCG AGGCCCCGGC CCAGGCGGTG 
GCGGTGCACA GCCAGGACCA GTCCATCCCC CCATGTCCGC AGACCTGGAG GAGCCTCTGG 
ATCGGGTATT CATTCCTGAT GCACACAGGA GCTGGGGACC AAGGAGGAGG GCAGGCCCTT 
ATGTCACCTG GCAGCTGCCT GGAAGATTTC AGAGCAGCAC CATTCCTTGA ATGCCAGGGC 
CGGCAGGGAA CTTGCCACTT TTTTGCAAAT AAGTATAGCT TCTGGCTCAC AACGGTGAAA 
GCAGACTTGC AGTTTTCCTC TGCTCCAGCA CCAGACACCT TAAAAGAAAG CCAGGCCCAA 
CGCCAGAAAA TCAGCCGGTG CCAGGTCTGC GTGAAGTATA GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL4A4 ( XM_009444468.3 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_009442743.3 Pan troglodytes collagen type IV alpha 4 chain (COL4A4), transcript variant X8, mRNA.

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CloneID	OPc104273
Clone ID Related Accession (Same CDS sequence)	XM_009444468.3
Accession Version	XM_009444468.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4884bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	collagen alpha-4(IV) chain isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036881.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_009444468.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGTCTC TGCACATAGT ACTAATGAGG TGCTCCTTCA GATTGACCAA GTCCTTGGCC 
ACAGATCCCT GGTCACTTAC ACTCATTCTC TTTTCTGTAC AATATGTATA TGGGAGTGGA 
AAGAAATACG TTGGTCCTTG TGGAGGAAGA GATTGCTCTG TTTGCCACTG TGTTCCTGAA 
AAGGGGTCTC GGGGTCCACC AGGACCACCA GGGCCACAGG GTCCAATTGG ACCCCTGGGA 
GCCCCAGGAC CCATTGGGCT TTCAGGAGAG AAAGGAATGA GAGGGGACCG CGGCCCTCCT 
GGAGCAGCAG GGGACAAAGG AGATAAGGGT TCAACTGGTG TTCCTGGATT TCCAGGTTTA 
GATGGCATAC CTGGGCACCC AGGGCCTCCT GGACCCAGAG GCAAACCTGG TATGAGTGGC 
CACAATGGCT CAAGAGGTGA CCCAGGGTTT CCAGGAGGAA GAGGAGCTCT TGGCCCAGGA 
GGCCCCCTAG GCCATCCTGG GGAAAAGGGA GAAAAAGGAA ATTCAGTGTT CATTTTAGGT 
GCCGTTAAAG GTATTCAGGG AGACAGAGGG GACCCAGGAC TGCCTGGCTT ACCAGGATCT 
TGGGGGGCAG GAGGACCGGC GGGTCCCACA GGATATCCTG GAGAGCCAGG GTTAGTGGGA 
CCTCCGGGCC AACCAGGGCG TCCAGGTTTG AAGGGAAATC CCGGTGTGGG AGTAAAGGGG 
CAAATGGGAG ACCCGGGTGA GGTTGGTCAG CAAGGTTCTC CTGGACCCAC CCTGTTGGTG 
GAGCCACCTG ACTTTCGTCT CTATAAAGGA GAAAAGGGTA TAAAAGGAAT TCCTGGAATG 
GTTGGACTGC CAGGACCACC AGGACGCAAG GGAGAATCTG ATATTGGGGC AAAAGGAGAA 
AAAGGTATTC CTGGATTTCC AGGGCCTCGG GGGGATCCTG GTTCCTATGG ATCTCCAGGT 
TTTCCAGGAT TAAAGGGAGA ACTAGGACTG GTTGGAGATC CTGGGCTATT TGGATTAATT 
GGCCCAAAGG GGGATCCTGG AAATCGAGGG CACCCAGGAC CACCAGGTGT TTTGGTGACT 
CCACCTCTTC CACTCAAAGG CCCACCAGGG GACCCAGGGT TCCCTGGCCG CTATGGAGAA 
ACAGGGGATG TTGGACCACC TGGTCCCCCA GGTCTCTTGG GCAGACCAGG GGAAGCCTGT 
GCAGGCATGA TAGGACCCCC TGGGCCACAA GGATTTCCTG GTCTTCCTGG GCTTCCAGGA 
GAAGCTGGTA TTCCTGGGAG ACCTGATTCT GCTCCAGGAA AACCAGGGAA GCCAGGATCA 
CCTGGCTTGC CTGGAGCACC AGGCCTGCAG GGCCTCCCAG GATCAAGTGT GATATACTGT 
AGTGTTGGGA ATCCTGGACC ACAAGGAATA AAAGGCAAAG TGGGTCCCCC AGGAGGAAGA 
GGCTCAAAAG GAGAAAAAGG AAATGAAGGA CTCTGTGCCT GTGAGCCTGG ACCCATGGGC 
CCCCCTGGCC CTCCAGGACT TCCTGGGAGG CAGGGGAGTA AGGGAGACTT GGGGCTCCCT 
GGCTGGCTTG GAACAAAAGG TGACCCAGGA CCTCCTGGTG CTGAAGGACC TCCAGGGCTA 
CCAGGAAAGC ATGGTGCCTC TGGACCACCT GGCAACAAAG GGGCGAAGGG TGACATGGTT 
GTATCAAGAG TTAAAGGGCA CAAAGGAGAA AGAGGTCCTG ATGGGCCCCC AGGATTTCCA 
GGGCAGCCAG GATCACATGG TCGGGATGGA CATGCTGGAG AAAAAGGGGA TCCAGGACCC 
CCAGGGGATC ATGAAGATGC GACCCCAGGT GGTAAAGGAT TTCCTGGACC TCTGGGCCCC 
CCGGGCAAAG CAGGACCTTT GGGGCCCCCA GGACTGGGAT TTCCTGGTCC ACCAGGACAG 
CGAGGCCACC CAGGAGTTCC AGGCCACCCA GGTGTGAGGG GCCCTGATGG CTTGAAGGGT 
CAGAAAGGTG ACACAATTTC TTGCAACGTA ACCTACCCTG GGAGGCACGG CCCTCCAGGT 
TTTGATGGAC CTCCAGGTCC GAAGGGATTT CCAGGTCCCC AAGGTGCCCC TGGGCTGAGT 
GGTTCAGATG GGCATAAAGG CAGACCTGGC ACACCAGGAA CAGCGGAAAT ACCAGGTCCA 
CCTGGTTTTC GTGGTGACAT GGGAGATCCG GGTTTTGGAG GTGAAAAGGG GTCCTCCCCT 
GCTGGGCCCC CAGGCCCTCC CGGCTCACCA GGAGTGAATG GTCAGAAAGG AATCCCGGGA 
GACCCTGCAT TTGGTCACCT GGGACCCCCG GGAAAGAGGG GTCTTTCAGG AGTGCCAGGG 
ATAAAAGGAC CCAGAGGTGA TCCGGGATGT CCAGGGGCTG AAGGGCCAGC TGGCATTCCT 
GGATTCCCAG GTCTCAAAGG TCCCAAAGGC AGAGAGGGAC ATGCTGGGTT TCCAGGTGTC 
CCAGGTCCAC CTGGCCATTC CTGTGAAAGA GGTGCTCCAG GGATACCAGG GCAACCGGGA 
CTCCCTGGGT ATCCAGGTAG CCCAGGTGCT CCAGGTGGGA AAGGACAGCT GGGAGATGTG 
GGGCCTCCCG GGCCAGCTGG AATGAAAGGC CTCCCTGGAC TCCCAGGATG GCCTGGGGCA 
CATGGTCCCC CAGGCCTCCC AGGAATCCCA GGTCCCTTTG GAGATGATGG GCTACCTGGT 
CCTCCAGGTC CAAAGGGACC CCAGGGGCTG CCTGGTTTCC CAGGTTTTCC CGGAGAAAGA 
GGAAAGCCTG GTGCAGAGGG ATGTCCTGGC ACAAAGGGAG AACCTGGAGA GAAGGGCATG 
TCTGGCCTTC CCGGAGACCG GGGACTGAGA GGGGCCAAAG GAGCCATAGG ACCTCCCGGA 
GATGAAGGAG AAATGGCTAT CATTTCCCAA AAGGGAACAC CTGGGGAACC TGGACCTCCT 
GGAGATGATG GATTCCCAGG AGAAAGAGGT GATAAAGGAA CTCCCGGGAT GCAAGGGAGA 
AGAGGAGAGC CGGGAAGATA CGGACCACCT GGATTTCAGA GAGGGGAACC TGGTGAGAAA 
GGTCAGCCAG GGCATCCTGG ACCCCCAGGC CCTCCAGGCT CAACTGGTCT AAGAGGGTTC 
ATTGGTTTTC CAGGACTTCC AGGTGACCAG GGTGAGCCAG GTTCTCCAGG TCCCCCTGGA 
TTTTCAGGAA TTGATGGAGC AAGAGGACCT AAAGGAAACA AAGGTGACCC TGCCAGTCAC 
TTTGGTCCAC CTGGTCCAAA GGGTGAGCCA GGTAGCCCTG GATGTCCAGG GCATTTTGGA 
GCATCTGGAG AGCAGGGCTT GCCTGGTATT CAAGGGCCCA GAGGATCACC TGGAAGGCCA 
GGGCCACCTG GCTCCTCTGG ACCACCAGGG TGCCCAGGTG ATCACGGGAT GCCTGGGCTG 
AGGGGACAGC CAGGAGAAAT GGGAGACCCT GGGCCAAGAG GCCTCCAGGG GGATCCAGGG 
ATACCAGGTC CTCCGGGAAT AAAAGGTCCC TCCGGATCAC CTGGCCTGAA CGGCTTGCAT 
GGATTGAAAG GTCAGAAAGG AACTAAAGGT GCTTCAGGTT TGCATGATGT GGGGCCACCT 
GGTCCAGTGG GAATACCTGG GCTAAAAGGG GAGAGAGGAG ACCCTGGGAG CCCAGGAATC 
TCTCCTCCAG GTCCTCGTGG AGAAAAAGGT CCCCCAGGAC CCCCAGGGAG TTCAGGACCA 
CCTGGTCCTG CAGGTGCCAC AGGAAGAGCT CCTAAGGACA TTCCTGACCC GGGTCCACCT 
GGAGATCAGG GACCTCCTGG TCCTGATGGC CCAAGAGGAG CACCTGGGCC TCCAGGCCTC 
CCTGGGAGTG TTGACCTTCT GAGAGGGGAG CCAGGTGACT GTGGTCTACC AGGGCCACCA 
GGTCCCCCTG GCCCACCAGG CCCTCCAGGA TACAAAGGCT TTCCAGGATG TGATGGAAAA 
GATGGCCAGA AAGGACCAAT GGGATTCCCG GGACCGCAGG GACCACATGG ATTTCCTGGG 
CCACCTGGAG AGAAGGGTTT ACCTGGACCT CCAGGGAGAA AAGGGCCCAC TGGTCTTCCA 
GGTCCCAGAG GTGAACCGGG GCCACCTGCA GATGTGGATG ACTGTCCCCG AATCCCAGGC 
CTTCCTGGGG CGCCAGGCAT GAGAGGACCA GAAGGAGCCA TGGGGCTCTC TGGAATGAGA 
GGCCCCCCAG GACCAGGGTG CAAAGGAGAG CCTGGGCTGG ATGGCAGGAG GGGTGTGGAT 
GGCGTCCCTG GGTCTCCTGG GCCTCCCGGA CGTAAAGGTG ACACAGGAGA AGACGGCTAC 
CCTGGAGGAC CAGGTCTGGC AGGGTCTTGC CTTCCCGTGT TTAGCACGCT GCCCTTTGCC 
TACTGCAACA TCCACCAGGT GTGCCACTAT GCCCAGAGAA ACGACAGATC CTACTGGCTG 
GCCAGCGCTG CGCCCCTCCC CATGATGCCA CTCTCTGAAG AGGCGATCCG CCCCTATGTC 
AGCCGCTGTG CGGTATGCGA GGCCCCGGCC CAGGCGGTGG CGGTGCACAG CCAGGACCAG 
TCCATCCCCC CATGTCCGCA GACCTGGAGG AGCCTCTGGA TCGGGTATTC ATTCCTGATG 
CACACAGGAG CTGGGGACCA AGGAGGAGGG CAGGCCCTTA TGTCACCTGG CAGCTGCCTG 
GAAGATTTCA GAGCAGCACC ATTCCTTGAA TGCCAGGGCC GGCAGGGAAC TTGCCACTTT 
TTTGCAAATA AGTATAGCTT CTGGCTCACA ACGGTGAAAG CAGACTTGCA GTTTTCCTCT 
GCTCCAGCAC CAGACACCTT AAAAGAAAGC CAGGCCCAAC GCCAGAAAAT CAGCCGGTGC 
CAGGTCTGCG TGAAGTATAG CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009442743.3
CDS	423..5306
Translation	MWSLHIVLMRCSFRLTKSLATDPWSLTLILFSVQYVYGSGKKYVGPCGGRDCSVCHCVPEKGSRGPPGPPGPQGPIGPLGAPGPIGLSGEKGMRGDRGPPGAAGDKGDKGSTGVPGFPGLDGIPGHPGPPGPRGKPGMSGHNGSRGDPGFPGGRGALGPGGPLGHPGEKGEKGNSVFILGAVKGIQGDRGDPGLPGLPGSWGAGGPAGPTGYPGEPGLVGPPGQPGRPGLKGNPGVGVKGQMGDPGEVGQQGSPGPTLLVEPPDFRLYKGEKGIKGIPGMVGLPGPPGRKGESDIGAKGEKGIPGFPGPRGDPGSYGSPGFPGLKGELGLVGDPGLFGLIGPKGDPGNRGHPGPPGVLVTPPLPLKGPPGDPGFPGRYGETGDVGPPGPPGLLGRPGEACAGMIGPPGPQGFPGLPGLPGEAGIPGRPDSAPGKPGKPGSPGLPGAPGLQGLPGSSVIYCSVGNPGPQGIKGKVGPPGGRGSKGEKGNEGLCACEPGPMGPPGPPGLPGRQGSKGDLGLPGWLGTKGDPGPPGAEGPPGLPGKHGASGPPGNKGAKGDMVVSRVKGHKGERGPDGPPGFPGQPGSHGRDGHAGEKGDPGPPGDHEDATPGGKGFPGPLGPPGKAGPLGPPGLGFPGPPGQRGHPGVPGHPGVRGPDGLKGQKGDTISCNVTYPGRHGPPGFDGPPGPKGFPGPQGAPGLSGSDGHKGRPGTPGTAEIPGPPGFRGDMGDPGFGGEKGSSPAGPPGPPGSPGVNGQKGIPGDPAFGHLGPPGKRGLSGVPGIKGPRGDPGCPGAEGPAGIPGFPGLKGPKGREGHAGFPGVPGPPGHSCERGAPGIPGQPGLPGYPGSPGAPGGKGQLGDVGPPGPAGMKGLPGLPGWPGAHGPPGLPGIPGPFGDDGLPGPPGPKGPQGLPGFPGFPGERGKPGAEGCPGTKGEPGEKGMSGLPGDRGLRGAKGAIGPPGDEGEMAIISQKGTPGEPGPPGDDGFPGERGDKGTPGMQGRRGEPGRYGPPGFQRGEPGEKGQPGHPGPPGPPGSTGLRGFIGFPGLPGDQGEPGSPGPPGFSGIDGARGPKGNKGDPASHFGPPGPKGEPGSPGCPGHFGASGEQGLPGIQGPRGSPGRPGPPGSSGPPGCPGDHGMPGLRGQPGEMGDPGPRGLQGDPGIPGPPGIKGPSGSPGLNGLHGLKGQKGTKGASGLHDVGPPGPVGIPGLKGERGDPGSPGISPPGPRGEKGPPGPPGSSGPPGPAGATGRAPKDIPDPGPPGDQGPPGPDGPRGAPGPPGLPGSVDLLRGEPGDCGLPGPPGPPGPPGPPGYKGFPGCDGKDGQKGPMGFPGPQGPHGFPGPPGEKGLPGPPGRKGPTGLPGPRGEPGPPADVDDCPRIPGLPGAPGMRGPEGAMGLSGMRGPPGPGCKGEPGLDGRRGVDGVPGSPGPPGRKGDTGEDGYPGGPGLAGSCLPVFSTLPFAYCNIHQVCHYAQRNDRSYWLASAAPLPMMPLSEEAIRPYVSRCAVCEAPAQAVAVHSQDQSIPPCPQTWRSLWIGYSFLMHTGAGDQGGGQALMSPGSCLEDFRAAPFLECQGRQGTCHFFANKYSFWLTTVKADLQFSSAPAPDTLKESQAQRQKISRCQVCVKYS

Target ORF information:

RefSeq Version	XM_009444468.3
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes collagen type IV alpha 4 chain (COL4A4), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009444468.3

ORF Insert Sequence:

ATGTGGTCTC TGCACATAGT ACTAATGAGG TGCTCCTTCA GATTGACCAA GTCCTTGGCC 
ACAGATCCCT GGTCACTTAC ACTCATTCTC TTTTCTGTAC AATATGTATA TGGGAGTGGA 
AAGAAATACG TTGGTCCTTG TGGAGGAAGA GATTGCTCTG TTTGCCACTG TGTTCCTGAA 
AAGGGGTCTC GGGGTCCACC AGGACCACCA GGGCCACAGG GTCCAATTGG ACCCCTGGGA 
GCCCCAGGAC CCATTGGGCT TTCAGGAGAG AAAGGAATGA GAGGGGACCG CGGCCCTCCT 
GGAGCAGCAG GGGACAAAGG AGATAAGGGT TCAACTGGTG TTCCTGGATT TCCAGGTTTA 
GATGGCATAC CTGGGCACCC AGGGCCTCCT GGACCCAGAG GCAAACCTGG TATGAGTGGC 
CACAATGGCT CAAGAGGTGA CCCAGGGTTT CCAGGAGGAA GAGGAGCTCT TGGCCCAGGA 
GGCCCCCTAG GCCATCCTGG GGAAAAGGGA GAAAAAGGAA ATTCAGTGTT CATTTTAGGT 
GCCGTTAAAG GTATTCAGGG AGACAGAGGG GACCCAGGAC TGCCTGGCTT ACCAGGATCT 
TGGGGGGCAG GAGGACCGGC GGGTCCCACA GGATATCCTG GAGAGCCAGG GTTAGTGGGA 
CCTCCGGGCC AACCAGGGCG TCCAGGTTTG AAGGGAAATC CCGGTGTGGG AGTAAAGGGG 
CAAATGGGAG ACCCGGGTGA GGTTGGTCAG CAAGGTTCTC CTGGACCCAC CCTGTTGGTG 
GAGCCACCTG ACTTTCGTCT CTATAAAGGA GAAAAGGGTA TAAAAGGAAT TCCTGGAATG 
GTTGGACTGC CAGGACCACC AGGACGCAAG GGAGAATCTG ATATTGGGGC AAAAGGAGAA 
AAAGGTATTC CTGGATTTCC AGGGCCTCGG GGGGATCCTG GTTCCTATGG ATCTCCAGGT 
TTTCCAGGAT TAAAGGGAGA ACTAGGACTG GTTGGAGATC CTGGGCTATT TGGATTAATT 
GGCCCAAAGG GGGATCCTGG AAATCGAGGG CACCCAGGAC CACCAGGTGT TTTGGTGACT 
CCACCTCTTC CACTCAAAGG CCCACCAGGG GACCCAGGGT TCCCTGGCCG CTATGGAGAA 
ACAGGGGATG TTGGACCACC TGGTCCCCCA GGTCTCTTGG GCAGACCAGG GGAAGCCTGT 
GCAGGCATGA TAGGACCCCC TGGGCCACAA GGATTTCCTG GTCTTCCTGG GCTTCCAGGA 
GAAGCTGGTA TTCCTGGGAG ACCTGATTCT GCTCCAGGAA AACCAGGGAA GCCAGGATCA 
CCTGGCTTGC CTGGAGCACC AGGCCTGCAG GGCCTCCCAG GATCAAGTGT GATATACTGT 
AGTGTTGGGA ATCCTGGACC ACAAGGAATA AAAGGCAAAG TGGGTCCCCC AGGAGGAAGA 
GGCTCAAAAG GAGAAAAAGG AAATGAAGGA CTCTGTGCCT GTGAGCCTGG ACCCATGGGC 
CCCCCTGGCC CTCCAGGACT TCCTGGGAGG CAGGGGAGTA AGGGAGACTT GGGGCTCCCT 
GGCTGGCTTG GAACAAAAGG TGACCCAGGA CCTCCTGGTG CTGAAGGACC TCCAGGGCTA 
CCAGGAAAGC ATGGTGCCTC TGGACCACCT GGCAACAAAG GGGCGAAGGG TGACATGGTT 
GTATCAAGAG TTAAAGGGCA CAAAGGAGAA AGAGGTCCTG ATGGGCCCCC AGGATTTCCA 
GGGCAGCCAG GATCACATGG TCGGGATGGA CATGCTGGAG AAAAAGGGGA TCCAGGACCC 
CCAGGGGATC ATGAAGATGC GACCCCAGGT GGTAAAGGAT TTCCTGGACC TCTGGGCCCC 
CCGGGCAAAG CAGGACCTTT GGGGCCCCCA GGACTGGGAT TTCCTGGTCC ACCAGGACAG 
CGAGGCCACC CAGGAGTTCC AGGCCACCCA GGTGTGAGGG GCCCTGATGG CTTGAAGGGT 
CAGAAAGGTG ACACAATTTC TTGCAACGTA ACCTACCCTG GGAGGCACGG CCCTCCAGGT 
TTTGATGGAC CTCCAGGTCC GAAGGGATTT CCAGGTCCCC AAGGTGCCCC TGGGCTGAGT 
GGTTCAGATG GGCATAAAGG CAGACCTGGC ACACCAGGAA CAGCGGAAAT ACCAGGTCCA 
CCTGGTTTTC GTGGTGACAT GGGAGATCCG GGTTTTGGAG GTGAAAAGGG GTCCTCCCCT 
GCTGGGCCCC CAGGCCCTCC CGGCTCACCA GGAGTGAATG GTCAGAAAGG AATCCCGGGA 
GACCCTGCAT TTGGTCACCT GGGACCCCCG GGAAAGAGGG GTCTTTCAGG AGTGCCAGGG 
ATAAAAGGAC CCAGAGGTGA TCCGGGATGT CCAGGGGCTG AAGGGCCAGC TGGCATTCCT 
GGATTCCCAG GTCTCAAAGG TCCCAAAGGC AGAGAGGGAC ATGCTGGGTT TCCAGGTGTC 
CCAGGTCCAC CTGGCCATTC CTGTGAAAGA GGTGCTCCAG GGATACCAGG GCAACCGGGA 
CTCCCTGGGT ATCCAGGTAG CCCAGGTGCT CCAGGTGGGA AAGGACAGCT GGGAGATGTG 
GGGCCTCCCG GGCCAGCTGG AATGAAAGGC CTCCCTGGAC TCCCAGGATG GCCTGGGGCA 
CATGGTCCCC CAGGCCTCCC AGGAATCCCA GGTCCCTTTG GAGATGATGG GCTACCTGGT 
CCTCCAGGTC CAAAGGGACC CCAGGGGCTG CCTGGTTTCC CAGGTTTTCC CGGAGAAAGA 
GGAAAGCCTG GTGCAGAGGG ATGTCCTGGC ACAAAGGGAG AACCTGGAGA GAAGGGCATG 
TCTGGCCTTC CCGGAGACCG GGGACTGAGA GGGGCCAAAG GAGCCATAGG ACCTCCCGGA 
GATGAAGGAG AAATGGCTAT CATTTCCCAA AAGGGAACAC CTGGGGAACC TGGACCTCCT 
GGAGATGATG GATTCCCAGG AGAAAGAGGT GATAAAGGAA CTCCCGGGAT GCAAGGGAGA 
AGAGGAGAGC CGGGAAGATA CGGACCACCT GGATTTCAGA GAGGGGAACC TGGTGAGAAA 
GGTCAGCCAG GGCATCCTGG ACCCCCAGGC CCTCCAGGCT CAACTGGTCT AAGAGGGTTC 
ATTGGTTTTC CAGGACTTCC AGGTGACCAG GGTGAGCCAG GTTCTCCAGG TCCCCCTGGA 
TTTTCAGGAA TTGATGGAGC AAGAGGACCT AAAGGAAACA AAGGTGACCC TGCCAGTCAC 
TTTGGTCCAC CTGGTCCAAA GGGTGAGCCA GGTAGCCCTG GATGTCCAGG GCATTTTGGA 
GCATCTGGAG AGCAGGGCTT GCCTGGTATT CAAGGGCCCA GAGGATCACC TGGAAGGCCA 
GGGCCACCTG GCTCCTCTGG ACCACCAGGG TGCCCAGGTG ATCACGGGAT GCCTGGGCTG 
AGGGGACAGC CAGGAGAAAT GGGAGACCCT GGGCCAAGAG GCCTCCAGGG GGATCCAGGG 
ATACCAGGTC CTCCGGGAAT AAAAGGTCCC TCCGGATCAC CTGGCCTGAA CGGCTTGCAT 
GGATTGAAAG GTCAGAAAGG AACTAAAGGT GCTTCAGGTT TGCATGATGT GGGGCCACCT 
GGTCCAGTGG GAATACCTGG GCTAAAAGGG GAGAGAGGAG ACCCTGGGAG CCCAGGAATC 
TCTCCTCCAG GTCCTCGTGG AGAAAAAGGT CCCCCAGGAC CCCCAGGGAG TTCAGGACCA 
CCTGGTCCTG CAGGTGCCAC AGGAAGAGCT CCTAAGGACA TTCCTGACCC GGGTCCACCT 
GGAGATCAGG GACCTCCTGG TCCTGATGGC CCAAGAGGAG CACCTGGGCC TCCAGGCCTC 
CCTGGGAGTG TTGACCTTCT GAGAGGGGAG CCAGGTGACT GTGGTCTACC AGGGCCACCA 
GGTCCCCCTG GCCCACCAGG CCCTCCAGGA TACAAAGGCT TTCCAGGATG TGATGGAAAA 
GATGGCCAGA AAGGACCAAT GGGATTCCCG GGACCGCAGG GACCACATGG ATTTCCTGGG 
CCACCTGGAG AGAAGGGTTT ACCTGGACCT CCAGGGAGAA AAGGGCCCAC TGGTCTTCCA 
GGTCCCAGAG GTGAACCGGG GCCACCTGCA GATGTGGATG ACTGTCCCCG AATCCCAGGC 
CTTCCTGGGG CGCCAGGCAT GAGAGGACCA GAAGGAGCCA TGGGGCTCTC TGGAATGAGA 
GGCCCCCCAG GACCAGGGTG CAAAGGAGAG CCTGGGCTGG ATGGCAGGAG GGGTGTGGAT 
GGCGTCCCTG GGTCTCCTGG GCCTCCCGGA CGTAAAGGTG ACACAGGAGA AGACGGCTAC 
CCTGGAGGAC CAGGTCTGGC AGGGTCTTGC CTTCCCGTGT TTAGCACGCT GCCCTTTGCC 
TACTGCAACA TCCACCAGGT GTGCCACTAT GCCCAGAGAA ACGACAGATC CTACTGGCTG 
GCCAGCGCTG CGCCCCTCCC CATGATGCCA CTCTCTGAAG AGGCGATCCG CCCCTATGTC 
AGCCGCTGTG CGGTATGCGA GGCCCCGGCC CAGGCGGTGG CGGTGCACAG CCAGGACCAG 
TCCATCCCCC CATGTCCGCA GACCTGGAGG AGCCTCTGGA TCGGGTATTC ATTCCTGATG 
CACACAGGAG CTGGGGACCA AGGAGGAGGG CAGGCCCTTA TGTCACCTGG CAGCTGCCTG 
GAAGATTTCA GAGCAGCACC ATTCCTTGAA TGCCAGGGCC GGCAGGGAAC TTGCCACTTT 
TTTGCAAATA AGTATAGCTT CTGGCTCACA ACGGTGAAAG CAGACTTGCA GTTTTCCTCT 
GCTCCAGCAC CAGACACCTT AAAAGAAAGC CAGGCCCAAC GCCAGAAAAT CAGCCGGTGC 
CAGGTCTGCG TGAAGTATAG CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL4A4 ( XM_009444469.3 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_009442744.3 Pan troglodytes collagen type IV alpha 4 chain (COL4A4), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OPc104274
Clone ID Related Accession (Same CDS sequence)	XM_009444469.3 , XM_009444470.3 , XM_016950593.2
Accession Version	XM_009444469.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4827bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	collagen alpha-4(IV) chain isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036881.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_009444469.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGTCTC TGCACATAGT ACTAATGAGG TGCTCCTTCA GATTGACCAA GTCCTTGGCC 
ACAGATCCCT GGTCACTTAC ACTCATTCTC TTTTCTGTAC AATATGTATA TGGGAGTGGA 
AAGAAATACG TTGGTCCTTG TGGAGGAAGA GATTGCTCTG TTTGCCACTG TGTTCCTGAA 
AAGGGGTCTC GGGGTCCACC AGGACCACCA GGGCCACAGG GTCCAATTGG ACCCCTGGGA 
GCCCCAGGAC CCATTGGGCT TTCAGGAGAG AAAGGAATGA GAGGGGACCG CGGCCCTCCT 
GGAGCAGCAG GGGACAAAGG AGATAAGGGT TCAACTGGTG TTCCTGGATT TCCAGGTTTA 
GATGGCATAC CTGGGCACCC AGGGCCTCCT GGACCCAGAG GCAAACCTGG TATGAGTGGC 
CACAATGGCT CAAGAGGTGA CCCAGGGTTT CCAGGAGGAA GAGGAGCTCT TGGCCCAGGA 
GGCCCCCTAG GCCATCCTGG GGAAAAGGGA GAAAAAGGAA ATTCAGTGTT CATTTTAGGT 
GCCGTTAAAG GTATTCAGGG AGACAGAGGG GACCCAGGAC TGCCTGGCTT ACCAGGATCT 
TGGGGGGCAG GAGGACCGGC GGGTCCCACA GGATATCCTG GAGAGCCAGG GTTAGTGGGA 
CCTCCGGGCC AACCAGGGCG TCCAGGTTTG AAGGGAAATC CCGGTGTGGG AGTAAAGGGG 
CAAATGGGAG ACCCGGGTGA GGTTGGTCAG CAAGGTTCTC CTGGACCCAC CCTGTTGGTG 
GAGCCACCTG ACTTTCGTCT CTATAAAGGA GAAAAGGGTA TAAAAGGAAT TCCTGGAATG 
GTTGGACTGC CAGGACCACC AGGACGCAAG GGAGAATCTG ATATTGGGGC AAAAGGAGAA 
AAAGGTATTC CTGGATTTCC AGGGCCTCGG GGGGATCCTG GTTCCTATGG ATCTCCAGGT 
TTTCCAGGAT TAAAGGGAGA ACTAGGACTG GTTGGAGATC CTGGGCTATT TGGATTAATT 
GGCCCAAAGG GGGATCCTGG AAATCGAGGG CACCCAGGAC CACCAGGTGT TTTGGTGACT 
CCACCTCTTC CACTCAAAGG CCCACCAGGG GACCCAGGGT TCCCTGGCCG CTATGGAGAA 
ACAGGGGATG TTGGACCACC TGGTCCCCCA GGTCTCTTGG GCAGACCAGG GGAAGCCTGT 
GCAGGCATGA TAGGACCCCC TGGGCCACAA GGATTTCCTG GTCTTCCTGG GCTTCCAGGA 
GAAGCTGGTA TTCCTGGGAG ACCTGATTCT GCTCCAGGAA AACCAGGGAA GCCAGGATCA 
CCTGGCTTGC CTGGAGCACC AGGCCTGCAG GGCCTCCCAG GATCAAGTGT GATATACTGT 
AGTGTTGGGA ATCCTGGACC ACAAGGAATA AAAGGCAAAG TGGGTCCCCC AGGAGGAAGA 
GGCTCAAAAG GAGAAAAAGG AAATGAAGGA CTCTGTGCCT GTGAGCCTGG ACCCATGGGC 
CCCCCTGGCC CTCCAGGACT TCCTGGGAGG CAGGGGAGTA AGGGAGACTT GGGGCTCCCT 
GGCTGGCTTG GAACAAAAGG TGACCCAGGA CCTCCTGGTG CTGAAGGACC TCCAGGGCTA 
CCAGGAAAGC ATGGTGCCTC TGGACCACCT GGCAACAAAG GGGCGAAGGG TGACATGGTT 
GTATCAAGAG TTAAAGGGCA CAAAGGAGAA AGAGGTCCTG ATGGGCCCCC AGGATTTCCA 
GGGCAGCCAG GATCACATGG TCGGGATGGA CATGCTGGAG AAAAAGGGGA TCCAGGACCC 
CCAGGGGATC ATGAAGATGC GACCCCAGGT GGTAAAGGAT TTCCTGGACC TCTGGGCCCC 
CCGGGCAAAG CAGGACCTTT GGGGCCCCCA GGACTGGGAT TTCCTGGTCC ACCAGGACAG 
CGAGGCCACC CAGGAGTTCC AGGCCACCCA GGTGTGAGGG GCCCTGATGG CTTGAAGGGT 
CAGAAAGGTG ACACAATTTC TTGCAACGTA ACCTACCCTG GGAGGCACGG CCCTCCAGGT 
TTTGATGGAC CTCCAGGTCC GAAGGGATTT CCAGGTCCCC AAGGTGCCCC TGGGCTGAGT 
GGTTCAGATG GGCATAAAGG CAGACCTGGC ACACCAGGAA CAGCGGAAAT ACCAGGTCCA 
CCTGGTTTTC GTGGTGACAT GGGAGATCCG GGTTTTGGAG GTGAAAAGGG GTCCTCCCCT 
GCTGGGCCCC CAGGCCCTCC CGGCTCACCA GGAGTGAATG GTCAGAAAGG AATCCCGGGA 
GACCCTGCAT TTGGTCACCT GGGACCCCCG GGAAAGAGGG GTCTTTCAGG AGTGCCAGGG 
ATAAAAGGAC CCAGAGGTGA TCCGGGATGT CCAGGGGCTG AAGGGCCAGC TGGCATTCCT 
GGATTCCCAG GTCTCAAAGG TCCCAAAGGC AGAGAGGGAC ATGCTGGGTT TCCAGGTGTC 
CCAGGTCCAC CTGGCCATTC CTGTGAAAGA GGTGCTCCAG GGATACCAGG GCAACCGGGA 
CTCCCTGGGT ATCCAGGTAG CCCAGGTGCT CCAGGTGGGA AAGGACAGCT GGGAGATGTG 
GGGCCTCCCG GGCCAGCTGG AATGAAAGGC CTCCCTGGAC TCCCAGGATG GCCTGGGGCA 
CATGGTCCCC CAGGCCTCCC AGGAATCCCA GGTCCCTTTG GAGATGATGG GCTACCTGGT 
CCTCCAGGTC CAAAGGGACC CCAGGGGCTG CCTGGTTTCC CAGGTTTTCC CGGAGAAAGA 
GGAAAGCCTG GTGCAGAGGG ATGTCCTGGC ACAAAGGGAG AACCTGGAGA GAAGGGCATG 
TCTGGCCTTC CCGGAGACCG GGGACTGAGA GGGGCCAAAG GAGCCATAGG ACCTCCCGGA 
GATGAAGGAG AAATGGCTAT CATTTCCCAA AAGGGAACAC CTGGGGAACC TGGACCTCCT 
GGAGATGATG GATTCCCAGG AGAAAGAGGT GATAAAGGAA CTCCCGGGAT GCAAGGGAGA 
AGAGGAGAGC CGGGAAGATA CGGACCACCT GGATTTCAGA GAGGGGAACC TGGTGAGAAA 
GGTCAGCCAG GGCATCCTGG ACCCCCAGGC CCTCCAGGCT CAACTGGTCT AAGAGGGTTC 
ATTGGTTTTC CAGGACTTCC AGGTGACCAG GGTGAGCCAG GTTCTCCAGG TCCCCCTGGA 
TTTTCAGGAA TTGATGGAGC AAGAGGACCT AAAGGAAACA AAGGTGACCC TGCCAGTCAC 
TTTGGTCCAC CTGGTCCAAA GGGTGAGCCA GGTAGCCCTG GATGTCCAGG GCATTTTGGA 
GCATCTGGAG AGCAGGGCTT GCCTGGTATT CAAGGGCCCA GAGGATCACC TGGAAGGCCA 
GGGCCACCTG GCTCCTCTGG ACCACCAGGG TGCCCAGGTG ATCACGGGAT GCCTGGGCTG 
AGGGGACAGC CAGGAGAAAT GGGAGACCCT GGGCCAAGAG GCCTCCAGGG GGATCCAGGG 
ATACCAGGTC CTCCGGGAAT AAAAGGTCCC TCCGGATCAC CTGGCCTGAA CGGCTTGCAT 
GGATTGAAAG GTCAGAAAGG AACTAAAGGT GCTTCAGGTT TGCATGATGT GGGGCCACCT 
GGTCCAGTGG GAATACCTGG GCTAAAAGGG GAGAGAGGAG ACCCTGGGAG CCCAGGAATC 
TCTCCTCCAG GTCCTCGTGG AGAAAAAGGT CCCCCAGGAC CCCCAGGGAG TTCAGGACCA 
CCTGGTCCTG CAGGTGCCAC AGGAAGAGCT CCTAAGGACA TTCCTGACCC GGGTCCACCT 
GGAGATCAGG GACCTCCTGG TCCTGATGGC CCAAGAGGAG CACCTGGGCC TCCAGGCCTC 
CCTGGGAGTG TTGACCTTCT GAGAGGGGAG CCAGGTGACT GTGGTCTACC AGGGCCACCA 
GGTCCCCCTG GCCCACCAGG CCCTCCAGGA TACAAAGGCT TTCCAGGATG TGATGGAAAA 
GATGGCCAGA AAGGACCAAT GGGATTCCCG GGACCGCAGG GACCACATGG ATTTCCTGGG 
CCACCTGGAG AGAAGGGTTT ACCTGGACCT CCAGGGAGAA AAGGGCCCAC TGGTCTTCCA 
GGTCCCAGAG GTGAACCGGG GCCACCTGCA GATGTGGATG ACTGTCCCCG AATCCCAGGC 
CTTCCTGGGG CGCCAGGCAT GAGAGGACCA GAAGGAGCCA TGGGGCTCTC TGGAATGAGA 
GGCCCCCCAG GACCAGGGTG CAAAGGAGAG CCTGGGCTGG ATGGCAGGAG GGGTGTGGAT 
GGCGTCCCTG GGTCTCCTGG GCCTCCCGGA CGTAAAGGTG ACACAGGAGA AGACGGCTAC 
CCTGGAGGAC CAGGGCCTCC TGGTCCCATT GGGGATCCTG GGCCCAAAGG GTTTGGCCCT 
GGATACCTCG GTGGCTTCCT CCTGGTTCTC CACAGTCAGA CGGACCAGGA GCCCACCTGC 
CCCCTGGGCA TGCCCAGGCT CTGGACTGGG TATAGTCTGT TATACCTGGA AGGGCAAGAG 
AAAGCTCACA ATCAAGACCT TGGTCTGGCA GGGTCTTGCC TTCCCGTGTT TAGCACGCTG 
CCCTTTGCCT ACTGCAACAT CCACCAGGTG TGCCACTATG CCCAGAGAAA CGACAGATCC 
TACTGGCTGG CCAGCGCTGC GCCCCTCCCC ATGATGCCAC TCTCTGAAGA GGCGATCCGC 
CCCTATGTCA GCCGCTGTGC GGTATGCGAG GCCCCGGCCC AGGCGGTGGC GGTGCACAGC 
CAGGACCAGT CCATCCCCCC ATGTCCGCAG ACCTGGAGGA GCCTCTGGAT CGGGTATTCA 
TTCCTGATGC TCATGGGTGT GTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009442744.3
CDS	423..5249
Translation	MWSLHIVLMRCSFRLTKSLATDPWSLTLILFSVQYVYGSGKKYVGPCGGRDCSVCHCVPEKGSRGPPGPPGPQGPIGPLGAPGPIGLSGEKGMRGDRGPPGAAGDKGDKGSTGVPGFPGLDGIPGHPGPPGPRGKPGMSGHNGSRGDPGFPGGRGALGPGGPLGHPGEKGEKGNSVFILGAVKGIQGDRGDPGLPGLPGSWGAGGPAGPTGYPGEPGLVGPPGQPGRPGLKGNPGVGVKGQMGDPGEVGQQGSPGPTLLVEPPDFRLYKGEKGIKGIPGMVGLPGPPGRKGESDIGAKGEKGIPGFPGPRGDPGSYGSPGFPGLKGELGLVGDPGLFGLIGPKGDPGNRGHPGPPGVLVTPPLPLKGPPGDPGFPGRYGETGDVGPPGPPGLLGRPGEACAGMIGPPGPQGFPGLPGLPGEAGIPGRPDSAPGKPGKPGSPGLPGAPGLQGLPGSSVIYCSVGNPGPQGIKGKVGPPGGRGSKGEKGNEGLCACEPGPMGPPGPPGLPGRQGSKGDLGLPGWLGTKGDPGPPGAEGPPGLPGKHGASGPPGNKGAKGDMVVSRVKGHKGERGPDGPPGFPGQPGSHGRDGHAGEKGDPGPPGDHEDATPGGKGFPGPLGPPGKAGPLGPPGLGFPGPPGQRGHPGVPGHPGVRGPDGLKGQKGDTISCNVTYPGRHGPPGFDGPPGPKGFPGPQGAPGLSGSDGHKGRPGTPGTAEIPGPPGFRGDMGDPGFGGEKGSSPAGPPGPPGSPGVNGQKGIPGDPAFGHLGPPGKRGLSGVPGIKGPRGDPGCPGAEGPAGIPGFPGLKGPKGREGHAGFPGVPGPPGHSCERGAPGIPGQPGLPGYPGSPGAPGGKGQLGDVGPPGPAGMKGLPGLPGWPGAHGPPGLPGIPGPFGDDGLPGPPGPKGPQGLPGFPGFPGERGKPGAEGCPGTKGEPGEKGMSGLPGDRGLRGAKGAIGPPGDEGEMAIISQKGTPGEPGPPGDDGFPGERGDKGTPGMQGRRGEPGRYGPPGFQRGEPGEKGQPGHPGPPGPPGSTGLRGFIGFPGLPGDQGEPGSPGPPGFSGIDGARGPKGNKGDPASHFGPPGPKGEPGSPGCPGHFGASGEQGLPGIQGPRGSPGRPGPPGSSGPPGCPGDHGMPGLRGQPGEMGDPGPRGLQGDPGIPGPPGIKGPSGSPGLNGLHGLKGQKGTKGASGLHDVGPPGPVGIPGLKGERGDPGSPGISPPGPRGEKGPPGPPGSSGPPGPAGATGRAPKDIPDPGPPGDQGPPGPDGPRGAPGPPGLPGSVDLLRGEPGDCGLPGPPGPPGPPGPPGYKGFPGCDGKDGQKGPMGFPGPQGPHGFPGPPGEKGLPGPPGRKGPTGLPGPRGEPGPPADVDDCPRIPGLPGAPGMRGPEGAMGLSGMRGPPGPGCKGEPGLDGRRGVDGVPGSPGPPGRKGDTGEDGYPGGPGPPGPIGDPGPKGFGPGYLGGFLLVLHSQTDQEPTCPLGMPRLWTGYSLLYLEGQEKAHNQDLGLAGSCLPVFSTLPFAYCNIHQVCHYAQRNDRSYWLASAAPLPMMPLSEEAIRPYVSRCAVCEAPAQAVAVHSQDQSIPPCPQTWRSLWIGYSFLMLMGVF

Target ORF information:

RefSeq Version	XM_009444469.3
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes collagen type IV alpha 4 chain (COL4A4), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009444469.3

ORF Insert Sequence:

ATGTGGTCTC TGCACATAGT ACTAATGAGG TGCTCCTTCA GATTGACCAA GTCCTTGGCC 
ACAGATCCCT GGTCACTTAC ACTCATTCTC TTTTCTGTAC AATATGTATA TGGGAGTGGA 
AAGAAATACG TTGGTCCTTG TGGAGGAAGA GATTGCTCTG TTTGCCACTG TGTTCCTGAA 
AAGGGGTCTC GGGGTCCACC AGGACCACCA GGGCCACAGG GTCCAATTGG ACCCCTGGGA 
GCCCCAGGAC CCATTGGGCT TTCAGGAGAG AAAGGAATGA GAGGGGACCG CGGCCCTCCT 
GGAGCAGCAG GGGACAAAGG AGATAAGGGT TCAACTGGTG TTCCTGGATT TCCAGGTTTA 
GATGGCATAC CTGGGCACCC AGGGCCTCCT GGACCCAGAG GCAAACCTGG TATGAGTGGC 
CACAATGGCT CAAGAGGTGA CCCAGGGTTT CCAGGAGGAA GAGGAGCTCT TGGCCCAGGA 
GGCCCCCTAG GCCATCCTGG GGAAAAGGGA GAAAAAGGAA ATTCAGTGTT CATTTTAGGT 
GCCGTTAAAG GTATTCAGGG AGACAGAGGG GACCCAGGAC TGCCTGGCTT ACCAGGATCT 
TGGGGGGCAG GAGGACCGGC GGGTCCCACA GGATATCCTG GAGAGCCAGG GTTAGTGGGA 
CCTCCGGGCC AACCAGGGCG TCCAGGTTTG AAGGGAAATC CCGGTGTGGG AGTAAAGGGG 
CAAATGGGAG ACCCGGGTGA GGTTGGTCAG CAAGGTTCTC CTGGACCCAC CCTGTTGGTG 
GAGCCACCTG ACTTTCGTCT CTATAAAGGA GAAAAGGGTA TAAAAGGAAT TCCTGGAATG 
GTTGGACTGC CAGGACCACC AGGACGCAAG GGAGAATCTG ATATTGGGGC AAAAGGAGAA 
AAAGGTATTC CTGGATTTCC AGGGCCTCGG GGGGATCCTG GTTCCTATGG ATCTCCAGGT 
TTTCCAGGAT TAAAGGGAGA ACTAGGACTG GTTGGAGATC CTGGGCTATT TGGATTAATT 
GGCCCAAAGG GGGATCCTGG AAATCGAGGG CACCCAGGAC CACCAGGTGT TTTGGTGACT 
CCACCTCTTC CACTCAAAGG CCCACCAGGG GACCCAGGGT TCCCTGGCCG CTATGGAGAA 
ACAGGGGATG TTGGACCACC TGGTCCCCCA GGTCTCTTGG GCAGACCAGG GGAAGCCTGT 
GCAGGCATGA TAGGACCCCC TGGGCCACAA GGATTTCCTG GTCTTCCTGG GCTTCCAGGA 
GAAGCTGGTA TTCCTGGGAG ACCTGATTCT GCTCCAGGAA AACCAGGGAA GCCAGGATCA 
CCTGGCTTGC CTGGAGCACC AGGCCTGCAG GGCCTCCCAG GATCAAGTGT GATATACTGT 
AGTGTTGGGA ATCCTGGACC ACAAGGAATA AAAGGCAAAG TGGGTCCCCC AGGAGGAAGA 
GGCTCAAAAG GAGAAAAAGG AAATGAAGGA CTCTGTGCCT GTGAGCCTGG ACCCATGGGC 
CCCCCTGGCC CTCCAGGACT TCCTGGGAGG CAGGGGAGTA AGGGAGACTT GGGGCTCCCT 
GGCTGGCTTG GAACAAAAGG TGACCCAGGA CCTCCTGGTG CTGAAGGACC TCCAGGGCTA 
CCAGGAAAGC ATGGTGCCTC TGGACCACCT GGCAACAAAG GGGCGAAGGG TGACATGGTT 
GTATCAAGAG TTAAAGGGCA CAAAGGAGAA AGAGGTCCTG ATGGGCCCCC AGGATTTCCA 
GGGCAGCCAG GATCACATGG TCGGGATGGA CATGCTGGAG AAAAAGGGGA TCCAGGACCC 
CCAGGGGATC ATGAAGATGC GACCCCAGGT GGTAAAGGAT TTCCTGGACC TCTGGGCCCC 
CCGGGCAAAG CAGGACCTTT GGGGCCCCCA GGACTGGGAT TTCCTGGTCC ACCAGGACAG 
CGAGGCCACC CAGGAGTTCC AGGCCACCCA GGTGTGAGGG GCCCTGATGG CTTGAAGGGT 
CAGAAAGGTG ACACAATTTC TTGCAACGTA ACCTACCCTG GGAGGCACGG CCCTCCAGGT 
TTTGATGGAC CTCCAGGTCC GAAGGGATTT CCAGGTCCCC AAGGTGCCCC TGGGCTGAGT 
GGTTCAGATG GGCATAAAGG CAGACCTGGC ACACCAGGAA CAGCGGAAAT ACCAGGTCCA 
CCTGGTTTTC GTGGTGACAT GGGAGATCCG GGTTTTGGAG GTGAAAAGGG GTCCTCCCCT 
GCTGGGCCCC CAGGCCCTCC CGGCTCACCA GGAGTGAATG GTCAGAAAGG AATCCCGGGA 
GACCCTGCAT TTGGTCACCT GGGACCCCCG GGAAAGAGGG GTCTTTCAGG AGTGCCAGGG 
ATAAAAGGAC CCAGAGGTGA TCCGGGATGT CCAGGGGCTG AAGGGCCAGC TGGCATTCCT 
GGATTCCCAG GTCTCAAAGG TCCCAAAGGC AGAGAGGGAC ATGCTGGGTT TCCAGGTGTC 
CCAGGTCCAC CTGGCCATTC CTGTGAAAGA GGTGCTCCAG GGATACCAGG GCAACCGGGA 
CTCCCTGGGT ATCCAGGTAG CCCAGGTGCT CCAGGTGGGA AAGGACAGCT GGGAGATGTG 
GGGCCTCCCG GGCCAGCTGG AATGAAAGGC CTCCCTGGAC TCCCAGGATG GCCTGGGGCA 
CATGGTCCCC CAGGCCTCCC AGGAATCCCA GGTCCCTTTG GAGATGATGG GCTACCTGGT 
CCTCCAGGTC CAAAGGGACC CCAGGGGCTG CCTGGTTTCC CAGGTTTTCC CGGAGAAAGA 
GGAAAGCCTG GTGCAGAGGG ATGTCCTGGC ACAAAGGGAG AACCTGGAGA GAAGGGCATG 
TCTGGCCTTC CCGGAGACCG GGGACTGAGA GGGGCCAAAG GAGCCATAGG ACCTCCCGGA 
GATGAAGGAG AAATGGCTAT CATTTCCCAA AAGGGAACAC CTGGGGAACC TGGACCTCCT 
GGAGATGATG GATTCCCAGG AGAAAGAGGT GATAAAGGAA CTCCCGGGAT GCAAGGGAGA 
AGAGGAGAGC CGGGAAGATA CGGACCACCT GGATTTCAGA GAGGGGAACC TGGTGAGAAA 
GGTCAGCCAG GGCATCCTGG ACCCCCAGGC CCTCCAGGCT CAACTGGTCT AAGAGGGTTC 
ATTGGTTTTC CAGGACTTCC AGGTGACCAG GGTGAGCCAG GTTCTCCAGG TCCCCCTGGA 
TTTTCAGGAA TTGATGGAGC AAGAGGACCT AAAGGAAACA AAGGTGACCC TGCCAGTCAC 
TTTGGTCCAC CTGGTCCAAA GGGTGAGCCA GGTAGCCCTG GATGTCCAGG GCATTTTGGA 
GCATCTGGAG AGCAGGGCTT GCCTGGTATT CAAGGGCCCA GAGGATCACC TGGAAGGCCA 
GGGCCACCTG GCTCCTCTGG ACCACCAGGG TGCCCAGGTG ATCACGGGAT GCCTGGGCTG 
AGGGGACAGC CAGGAGAAAT GGGAGACCCT GGGCCAAGAG GCCTCCAGGG GGATCCAGGG 
ATACCAGGTC CTCCGGGAAT AAAAGGTCCC TCCGGATCAC CTGGCCTGAA CGGCTTGCAT 
GGATTGAAAG GTCAGAAAGG AACTAAAGGT GCTTCAGGTT TGCATGATGT GGGGCCACCT 
GGTCCAGTGG GAATACCTGG GCTAAAAGGG GAGAGAGGAG ACCCTGGGAG CCCAGGAATC 
TCTCCTCCAG GTCCTCGTGG AGAAAAAGGT CCCCCAGGAC CCCCAGGGAG TTCAGGACCA 
CCTGGTCCTG CAGGTGCCAC AGGAAGAGCT CCTAAGGACA TTCCTGACCC GGGTCCACCT 
GGAGATCAGG GACCTCCTGG TCCTGATGGC CCAAGAGGAG CACCTGGGCC TCCAGGCCTC 
CCTGGGAGTG TTGACCTTCT GAGAGGGGAG CCAGGTGACT GTGGTCTACC AGGGCCACCA 
GGTCCCCCTG GCCCACCAGG CCCTCCAGGA TACAAAGGCT TTCCAGGATG TGATGGAAAA 
GATGGCCAGA AAGGACCAAT GGGATTCCCG GGACCGCAGG GACCACATGG ATTTCCTGGG 
CCACCTGGAG AGAAGGGTTT ACCTGGACCT CCAGGGAGAA AAGGGCCCAC TGGTCTTCCA 
GGTCCCAGAG GTGAACCGGG GCCACCTGCA GATGTGGATG ACTGTCCCCG AATCCCAGGC 
CTTCCTGGGG CGCCAGGCAT GAGAGGACCA GAAGGAGCCA TGGGGCTCTC TGGAATGAGA 
GGCCCCCCAG GACCAGGGTG CAAAGGAGAG CCTGGGCTGG ATGGCAGGAG GGGTGTGGAT 
GGCGTCCCTG GGTCTCCTGG GCCTCCCGGA CGTAAAGGTG ACACAGGAGA AGACGGCTAC 
CCTGGAGGAC CAGGGCCTCC TGGTCCCATT GGGGATCCTG GGCCCAAAGG GTTTGGCCCT 
GGATACCTCG GTGGCTTCCT CCTGGTTCTC CACAGTCAGA CGGACCAGGA GCCCACCTGC 
CCCCTGGGCA TGCCCAGGCT CTGGACTGGG TATAGTCTGT TATACCTGGA AGGGCAAGAG 
AAAGCTCACA ATCAAGACCT TGGTCTGGCA GGGTCTTGCC TTCCCGTGTT TAGCACGCTG 
CCCTTTGCCT ACTGCAACAT CCACCAGGTG TGCCACTATG CCCAGAGAAA CGACAGATCC 
TACTGGCTGG CCAGCGCTGC GCCCCTCCCC ATGATGCCAC TCTCTGAAGA GGCGATCCGC 
CCCTATGTCA GCCGCTGTGC GGTATGCGAG GCCCCGGCCC AGGCGGTGGC GGTGCACAGC 
CAGGACCAGT CCATCCCCCC ATGTCCGCAG ACCTGGAGGA GCCTCTGGAT CGGGTATTCA 
TTCCTGATGC TCATGGGTGT GTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL4A4 ( XM_009444470.3 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_009442745.3 Pan troglodytes collagen type IV alpha 4 chain (COL4A4), transcript variant X11, mRNA.

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CloneID	OPc104274
Clone ID Related Accession (Same CDS sequence)	XM_009444469.3 , XM_009444470.3 , XM_016950593.2
Accession Version	XM_009444470.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4827bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	collagen alpha-4(IV) chain isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036881.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_009444470.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGTCTC TGCACATAGT ACTAATGAGG TGCTCCTTCA GATTGACCAA GTCCTTGGCC 
ACAGATCCCT GGTCACTTAC ACTCATTCTC TTTTCTGTAC AATATGTATA TGGGAGTGGA 
AAGAAATACG TTGGTCCTTG TGGAGGAAGA GATTGCTCTG TTTGCCACTG TGTTCCTGAA 
AAGGGGTCTC GGGGTCCACC AGGACCACCA GGGCCACAGG GTCCAATTGG ACCCCTGGGA 
GCCCCAGGAC CCATTGGGCT TTCAGGAGAG AAAGGAATGA GAGGGGACCG CGGCCCTCCT 
GGAGCAGCAG GGGACAAAGG AGATAAGGGT TCAACTGGTG TTCCTGGATT TCCAGGTTTA 
GATGGCATAC CTGGGCACCC AGGGCCTCCT GGACCCAGAG GCAAACCTGG TATGAGTGGC 
CACAATGGCT CAAGAGGTGA CCCAGGGTTT CCAGGAGGAA GAGGAGCTCT TGGCCCAGGA 
GGCCCCCTAG GCCATCCTGG GGAAAAGGGA GAAAAAGGAA ATTCAGTGTT CATTTTAGGT 
GCCGTTAAAG GTATTCAGGG AGACAGAGGG GACCCAGGAC TGCCTGGCTT ACCAGGATCT 
TGGGGGGCAG GAGGACCGGC GGGTCCCACA GGATATCCTG GAGAGCCAGG GTTAGTGGGA 
CCTCCGGGCC AACCAGGGCG TCCAGGTTTG AAGGGAAATC CCGGTGTGGG AGTAAAGGGG 
CAAATGGGAG ACCCGGGTGA GGTTGGTCAG CAAGGTTCTC CTGGACCCAC CCTGTTGGTG 
GAGCCACCTG ACTTTCGTCT CTATAAAGGA GAAAAGGGTA TAAAAGGAAT TCCTGGAATG 
GTTGGACTGC CAGGACCACC AGGACGCAAG GGAGAATCTG ATATTGGGGC AAAAGGAGAA 
AAAGGTATTC CTGGATTTCC AGGGCCTCGG GGGGATCCTG GTTCCTATGG ATCTCCAGGT 
TTTCCAGGAT TAAAGGGAGA ACTAGGACTG GTTGGAGATC CTGGGCTATT TGGATTAATT 
GGCCCAAAGG GGGATCCTGG AAATCGAGGG CACCCAGGAC CACCAGGTGT TTTGGTGACT 
CCACCTCTTC CACTCAAAGG CCCACCAGGG GACCCAGGGT TCCCTGGCCG CTATGGAGAA 
ACAGGGGATG TTGGACCACC TGGTCCCCCA GGTCTCTTGG GCAGACCAGG GGAAGCCTGT 
GCAGGCATGA TAGGACCCCC TGGGCCACAA GGATTTCCTG GTCTTCCTGG GCTTCCAGGA 
GAAGCTGGTA TTCCTGGGAG ACCTGATTCT GCTCCAGGAA AACCAGGGAA GCCAGGATCA 
CCTGGCTTGC CTGGAGCACC AGGCCTGCAG GGCCTCCCAG GATCAAGTGT GATATACTGT 
AGTGTTGGGA ATCCTGGACC ACAAGGAATA AAAGGCAAAG TGGGTCCCCC AGGAGGAAGA 
GGCTCAAAAG GAGAAAAAGG AAATGAAGGA CTCTGTGCCT GTGAGCCTGG ACCCATGGGC 
CCCCCTGGCC CTCCAGGACT TCCTGGGAGG CAGGGGAGTA AGGGAGACTT GGGGCTCCCT 
GGCTGGCTTG GAACAAAAGG TGACCCAGGA CCTCCTGGTG CTGAAGGACC TCCAGGGCTA 
CCAGGAAAGC ATGGTGCCTC TGGACCACCT GGCAACAAAG GGGCGAAGGG TGACATGGTT 
GTATCAAGAG TTAAAGGGCA CAAAGGAGAA AGAGGTCCTG ATGGGCCCCC AGGATTTCCA 
GGGCAGCCAG GATCACATGG TCGGGATGGA CATGCTGGAG AAAAAGGGGA TCCAGGACCC 
CCAGGGGATC ATGAAGATGC GACCCCAGGT GGTAAAGGAT TTCCTGGACC TCTGGGCCCC 
CCGGGCAAAG CAGGACCTTT GGGGCCCCCA GGACTGGGAT TTCCTGGTCC ACCAGGACAG 
CGAGGCCACC CAGGAGTTCC AGGCCACCCA GGTGTGAGGG GCCCTGATGG CTTGAAGGGT 
CAGAAAGGTG ACACAATTTC TTGCAACGTA ACCTACCCTG GGAGGCACGG CCCTCCAGGT 
TTTGATGGAC CTCCAGGTCC GAAGGGATTT CCAGGTCCCC AAGGTGCCCC TGGGCTGAGT 
GGTTCAGATG GGCATAAAGG CAGACCTGGC ACACCAGGAA CAGCGGAAAT ACCAGGTCCA 
CCTGGTTTTC GTGGTGACAT GGGAGATCCG GGTTTTGGAG GTGAAAAGGG GTCCTCCCCT 
GCTGGGCCCC CAGGCCCTCC CGGCTCACCA GGAGTGAATG GTCAGAAAGG AATCCCGGGA 
GACCCTGCAT TTGGTCACCT GGGACCCCCG GGAAAGAGGG GTCTTTCAGG AGTGCCAGGG 
ATAAAAGGAC CCAGAGGTGA TCCGGGATGT CCAGGGGCTG AAGGGCCAGC TGGCATTCCT 
GGATTCCCAG GTCTCAAAGG TCCCAAAGGC AGAGAGGGAC ATGCTGGGTT TCCAGGTGTC 
CCAGGTCCAC CTGGCCATTC CTGTGAAAGA GGTGCTCCAG GGATACCAGG GCAACCGGGA 
CTCCCTGGGT ATCCAGGTAG CCCAGGTGCT CCAGGTGGGA AAGGACAGCT GGGAGATGTG 
GGGCCTCCCG GGCCAGCTGG AATGAAAGGC CTCCCTGGAC TCCCAGGATG GCCTGGGGCA 
CATGGTCCCC CAGGCCTCCC AGGAATCCCA GGTCCCTTTG GAGATGATGG GCTACCTGGT 
CCTCCAGGTC CAAAGGGACC CCAGGGGCTG CCTGGTTTCC CAGGTTTTCC CGGAGAAAGA 
GGAAAGCCTG GTGCAGAGGG ATGTCCTGGC ACAAAGGGAG AACCTGGAGA GAAGGGCATG 
TCTGGCCTTC CCGGAGACCG GGGACTGAGA GGGGCCAAAG GAGCCATAGG ACCTCCCGGA 
GATGAAGGAG AAATGGCTAT CATTTCCCAA AAGGGAACAC CTGGGGAACC TGGACCTCCT 
GGAGATGATG GATTCCCAGG AGAAAGAGGT GATAAAGGAA CTCCCGGGAT GCAAGGGAGA 
AGAGGAGAGC CGGGAAGATA CGGACCACCT GGATTTCAGA GAGGGGAACC TGGTGAGAAA 
GGTCAGCCAG GGCATCCTGG ACCCCCAGGC CCTCCAGGCT CAACTGGTCT AAGAGGGTTC 
ATTGGTTTTC CAGGACTTCC AGGTGACCAG GGTGAGCCAG GTTCTCCAGG TCCCCCTGGA 
TTTTCAGGAA TTGATGGAGC AAGAGGACCT AAAGGAAACA AAGGTGACCC TGCCAGTCAC 
TTTGGTCCAC CTGGTCCAAA GGGTGAGCCA GGTAGCCCTG GATGTCCAGG GCATTTTGGA 
GCATCTGGAG AGCAGGGCTT GCCTGGTATT CAAGGGCCCA GAGGATCACC TGGAAGGCCA 
GGGCCACCTG GCTCCTCTGG ACCACCAGGG TGCCCAGGTG ATCACGGGAT GCCTGGGCTG 
AGGGGACAGC CAGGAGAAAT GGGAGACCCT GGGCCAAGAG GCCTCCAGGG GGATCCAGGG 
ATACCAGGTC CTCCGGGAAT AAAAGGTCCC TCCGGATCAC CTGGCCTGAA CGGCTTGCAT 
GGATTGAAAG GTCAGAAAGG AACTAAAGGT GCTTCAGGTT TGCATGATGT GGGGCCACCT 
GGTCCAGTGG GAATACCTGG GCTAAAAGGG GAGAGAGGAG ACCCTGGGAG CCCAGGAATC 
TCTCCTCCAG GTCCTCGTGG AGAAAAAGGT CCCCCAGGAC CCCCAGGGAG TTCAGGACCA 
CCTGGTCCTG CAGGTGCCAC AGGAAGAGCT CCTAAGGACA TTCCTGACCC GGGTCCACCT 
GGAGATCAGG GACCTCCTGG TCCTGATGGC CCAAGAGGAG CACCTGGGCC TCCAGGCCTC 
CCTGGGAGTG TTGACCTTCT GAGAGGGGAG CCAGGTGACT GTGGTCTACC AGGGCCACCA 
GGTCCCCCTG GCCCACCAGG CCCTCCAGGA TACAAAGGCT TTCCAGGATG TGATGGAAAA 
GATGGCCAGA AAGGACCAAT GGGATTCCCG GGACCGCAGG GACCACATGG ATTTCCTGGG 
CCACCTGGAG AGAAGGGTTT ACCTGGACCT CCAGGGAGAA AAGGGCCCAC TGGTCTTCCA 
GGTCCCAGAG GTGAACCGGG GCCACCTGCA GATGTGGATG ACTGTCCCCG AATCCCAGGC 
CTTCCTGGGG CGCCAGGCAT GAGAGGACCA GAAGGAGCCA TGGGGCTCTC TGGAATGAGA 
GGCCCCCCAG GACCAGGGTG CAAAGGAGAG CCTGGGCTGG ATGGCAGGAG GGGTGTGGAT 
GGCGTCCCTG GGTCTCCTGG GCCTCCCGGA CGTAAAGGTG ACACAGGAGA AGACGGCTAC 
CCTGGAGGAC CAGGGCCTCC TGGTCCCATT GGGGATCCTG GGCCCAAAGG GTTTGGCCCT 
GGATACCTCG GTGGCTTCCT CCTGGTTCTC CACAGTCAGA CGGACCAGGA GCCCACCTGC 
CCCCTGGGCA TGCCCAGGCT CTGGACTGGG TATAGTCTGT TATACCTGGA AGGGCAAGAG 
AAAGCTCACA ATCAAGACCT TGGTCTGGCA GGGTCTTGCC TTCCCGTGTT TAGCACGCTG 
CCCTTTGCCT ACTGCAACAT CCACCAGGTG TGCCACTATG CCCAGAGAAA CGACAGATCC 
TACTGGCTGG CCAGCGCTGC GCCCCTCCCC ATGATGCCAC TCTCTGAAGA GGCGATCCGC 
CCCTATGTCA GCCGCTGTGC GGTATGCGAG GCCCCGGCCC AGGCGGTGGC GGTGCACAGC 
CAGGACCAGT CCATCCCCCC ATGTCCGCAG ACCTGGAGGA GCCTCTGGAT CGGGTATTCA 
TTCCTGATGC TCATGGGTGT GTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009442745.3
CDS	423..5249
Translation	MWSLHIVLMRCSFRLTKSLATDPWSLTLILFSVQYVYGSGKKYVGPCGGRDCSVCHCVPEKGSRGPPGPPGPQGPIGPLGAPGPIGLSGEKGMRGDRGPPGAAGDKGDKGSTGVPGFPGLDGIPGHPGPPGPRGKPGMSGHNGSRGDPGFPGGRGALGPGGPLGHPGEKGEKGNSVFILGAVKGIQGDRGDPGLPGLPGSWGAGGPAGPTGYPGEPGLVGPPGQPGRPGLKGNPGVGVKGQMGDPGEVGQQGSPGPTLLVEPPDFRLYKGEKGIKGIPGMVGLPGPPGRKGESDIGAKGEKGIPGFPGPRGDPGSYGSPGFPGLKGELGLVGDPGLFGLIGPKGDPGNRGHPGPPGVLVTPPLPLKGPPGDPGFPGRYGETGDVGPPGPPGLLGRPGEACAGMIGPPGPQGFPGLPGLPGEAGIPGRPDSAPGKPGKPGSPGLPGAPGLQGLPGSSVIYCSVGNPGPQGIKGKVGPPGGRGSKGEKGNEGLCACEPGPMGPPGPPGLPGRQGSKGDLGLPGWLGTKGDPGPPGAEGPPGLPGKHGASGPPGNKGAKGDMVVSRVKGHKGERGPDGPPGFPGQPGSHGRDGHAGEKGDPGPPGDHEDATPGGKGFPGPLGPPGKAGPLGPPGLGFPGPPGQRGHPGVPGHPGVRGPDGLKGQKGDTISCNVTYPGRHGPPGFDGPPGPKGFPGPQGAPGLSGSDGHKGRPGTPGTAEIPGPPGFRGDMGDPGFGGEKGSSPAGPPGPPGSPGVNGQKGIPGDPAFGHLGPPGKRGLSGVPGIKGPRGDPGCPGAEGPAGIPGFPGLKGPKGREGHAGFPGVPGPPGHSCERGAPGIPGQPGLPGYPGSPGAPGGKGQLGDVGPPGPAGMKGLPGLPGWPGAHGPPGLPGIPGPFGDDGLPGPPGPKGPQGLPGFPGFPGERGKPGAEGCPGTKGEPGEKGMSGLPGDRGLRGAKGAIGPPGDEGEMAIISQKGTPGEPGPPGDDGFPGERGDKGTPGMQGRRGEPGRYGPPGFQRGEPGEKGQPGHPGPPGPPGSTGLRGFIGFPGLPGDQGEPGSPGPPGFSGIDGARGPKGNKGDPASHFGPPGPKGEPGSPGCPGHFGASGEQGLPGIQGPRGSPGRPGPPGSSGPPGCPGDHGMPGLRGQPGEMGDPGPRGLQGDPGIPGPPGIKGPSGSPGLNGLHGLKGQKGTKGASGLHDVGPPGPVGIPGLKGERGDPGSPGISPPGPRGEKGPPGPPGSSGPPGPAGATGRAPKDIPDPGPPGDQGPPGPDGPRGAPGPPGLPGSVDLLRGEPGDCGLPGPPGPPGPPGPPGYKGFPGCDGKDGQKGPMGFPGPQGPHGFPGPPGEKGLPGPPGRKGPTGLPGPRGEPGPPADVDDCPRIPGLPGAPGMRGPEGAMGLSGMRGPPGPGCKGEPGLDGRRGVDGVPGSPGPPGRKGDTGEDGYPGGPGPPGPIGDPGPKGFGPGYLGGFLLVLHSQTDQEPTCPLGMPRLWTGYSLLYLEGQEKAHNQDLGLAGSCLPVFSTLPFAYCNIHQVCHYAQRNDRSYWLASAAPLPMMPLSEEAIRPYVSRCAVCEAPAQAVAVHSQDQSIPPCPQTWRSLWIGYSFLMLMGVF

Target ORF information:

RefSeq Version	XM_009444470.3
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes collagen type IV alpha 4 chain (COL4A4), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009444470.3

ORF Insert Sequence:

ATGTGGTCTC TGCACATAGT ACTAATGAGG TGCTCCTTCA GATTGACCAA GTCCTTGGCC 
ACAGATCCCT GGTCACTTAC ACTCATTCTC TTTTCTGTAC AATATGTATA TGGGAGTGGA 
AAGAAATACG TTGGTCCTTG TGGAGGAAGA GATTGCTCTG TTTGCCACTG TGTTCCTGAA 
AAGGGGTCTC GGGGTCCACC AGGACCACCA GGGCCACAGG GTCCAATTGG ACCCCTGGGA 
GCCCCAGGAC CCATTGGGCT TTCAGGAGAG AAAGGAATGA GAGGGGACCG CGGCCCTCCT 
GGAGCAGCAG GGGACAAAGG AGATAAGGGT TCAACTGGTG TTCCTGGATT TCCAGGTTTA 
GATGGCATAC CTGGGCACCC AGGGCCTCCT GGACCCAGAG GCAAACCTGG TATGAGTGGC 
CACAATGGCT CAAGAGGTGA CCCAGGGTTT CCAGGAGGAA GAGGAGCTCT TGGCCCAGGA 
GGCCCCCTAG GCCATCCTGG GGAAAAGGGA GAAAAAGGAA ATTCAGTGTT CATTTTAGGT 
GCCGTTAAAG GTATTCAGGG AGACAGAGGG GACCCAGGAC TGCCTGGCTT ACCAGGATCT 
TGGGGGGCAG GAGGACCGGC GGGTCCCACA GGATATCCTG GAGAGCCAGG GTTAGTGGGA 
CCTCCGGGCC AACCAGGGCG TCCAGGTTTG AAGGGAAATC CCGGTGTGGG AGTAAAGGGG 
CAAATGGGAG ACCCGGGTGA GGTTGGTCAG CAAGGTTCTC CTGGACCCAC CCTGTTGGTG 
GAGCCACCTG ACTTTCGTCT CTATAAAGGA GAAAAGGGTA TAAAAGGAAT TCCTGGAATG 
GTTGGACTGC CAGGACCACC AGGACGCAAG GGAGAATCTG ATATTGGGGC AAAAGGAGAA 
AAAGGTATTC CTGGATTTCC AGGGCCTCGG GGGGATCCTG GTTCCTATGG ATCTCCAGGT 
TTTCCAGGAT TAAAGGGAGA ACTAGGACTG GTTGGAGATC CTGGGCTATT TGGATTAATT 
GGCCCAAAGG GGGATCCTGG AAATCGAGGG CACCCAGGAC CACCAGGTGT TTTGGTGACT 
CCACCTCTTC CACTCAAAGG CCCACCAGGG GACCCAGGGT TCCCTGGCCG CTATGGAGAA 
ACAGGGGATG TTGGACCACC TGGTCCCCCA GGTCTCTTGG GCAGACCAGG GGAAGCCTGT 
GCAGGCATGA TAGGACCCCC TGGGCCACAA GGATTTCCTG GTCTTCCTGG GCTTCCAGGA 
GAAGCTGGTA TTCCTGGGAG ACCTGATTCT GCTCCAGGAA AACCAGGGAA GCCAGGATCA 
CCTGGCTTGC CTGGAGCACC AGGCCTGCAG GGCCTCCCAG GATCAAGTGT GATATACTGT 
AGTGTTGGGA ATCCTGGACC ACAAGGAATA AAAGGCAAAG TGGGTCCCCC AGGAGGAAGA 
GGCTCAAAAG GAGAAAAAGG AAATGAAGGA CTCTGTGCCT GTGAGCCTGG ACCCATGGGC 
CCCCCTGGCC CTCCAGGACT TCCTGGGAGG CAGGGGAGTA AGGGAGACTT GGGGCTCCCT 
GGCTGGCTTG GAACAAAAGG TGACCCAGGA CCTCCTGGTG CTGAAGGACC TCCAGGGCTA 
CCAGGAAAGC ATGGTGCCTC TGGACCACCT GGCAACAAAG GGGCGAAGGG TGACATGGTT 
GTATCAAGAG TTAAAGGGCA CAAAGGAGAA AGAGGTCCTG ATGGGCCCCC AGGATTTCCA 
GGGCAGCCAG GATCACATGG TCGGGATGGA CATGCTGGAG AAAAAGGGGA TCCAGGACCC 
CCAGGGGATC ATGAAGATGC GACCCCAGGT GGTAAAGGAT TTCCTGGACC TCTGGGCCCC 
CCGGGCAAAG CAGGACCTTT GGGGCCCCCA GGACTGGGAT TTCCTGGTCC ACCAGGACAG 
CGAGGCCACC CAGGAGTTCC AGGCCACCCA GGTGTGAGGG GCCCTGATGG CTTGAAGGGT 
CAGAAAGGTG ACACAATTTC TTGCAACGTA ACCTACCCTG GGAGGCACGG CCCTCCAGGT 
TTTGATGGAC CTCCAGGTCC GAAGGGATTT CCAGGTCCCC AAGGTGCCCC TGGGCTGAGT 
GGTTCAGATG GGCATAAAGG CAGACCTGGC ACACCAGGAA CAGCGGAAAT ACCAGGTCCA 
CCTGGTTTTC GTGGTGACAT GGGAGATCCG GGTTTTGGAG GTGAAAAGGG GTCCTCCCCT 
GCTGGGCCCC CAGGCCCTCC CGGCTCACCA GGAGTGAATG GTCAGAAAGG AATCCCGGGA 
GACCCTGCAT TTGGTCACCT GGGACCCCCG GGAAAGAGGG GTCTTTCAGG AGTGCCAGGG 
ATAAAAGGAC CCAGAGGTGA TCCGGGATGT CCAGGGGCTG AAGGGCCAGC TGGCATTCCT 
GGATTCCCAG GTCTCAAAGG TCCCAAAGGC AGAGAGGGAC ATGCTGGGTT TCCAGGTGTC 
CCAGGTCCAC CTGGCCATTC CTGTGAAAGA GGTGCTCCAG GGATACCAGG GCAACCGGGA 
CTCCCTGGGT ATCCAGGTAG CCCAGGTGCT CCAGGTGGGA AAGGACAGCT GGGAGATGTG 
GGGCCTCCCG GGCCAGCTGG AATGAAAGGC CTCCCTGGAC TCCCAGGATG GCCTGGGGCA 
CATGGTCCCC CAGGCCTCCC AGGAATCCCA GGTCCCTTTG GAGATGATGG GCTACCTGGT 
CCTCCAGGTC CAAAGGGACC CCAGGGGCTG CCTGGTTTCC CAGGTTTTCC CGGAGAAAGA 
GGAAAGCCTG GTGCAGAGGG ATGTCCTGGC ACAAAGGGAG AACCTGGAGA GAAGGGCATG 
TCTGGCCTTC CCGGAGACCG GGGACTGAGA GGGGCCAAAG GAGCCATAGG ACCTCCCGGA 
GATGAAGGAG AAATGGCTAT CATTTCCCAA AAGGGAACAC CTGGGGAACC TGGACCTCCT 
GGAGATGATG GATTCCCAGG AGAAAGAGGT GATAAAGGAA CTCCCGGGAT GCAAGGGAGA 
AGAGGAGAGC CGGGAAGATA CGGACCACCT GGATTTCAGA GAGGGGAACC TGGTGAGAAA 
GGTCAGCCAG GGCATCCTGG ACCCCCAGGC CCTCCAGGCT CAACTGGTCT AAGAGGGTTC 
ATTGGTTTTC CAGGACTTCC AGGTGACCAG GGTGAGCCAG GTTCTCCAGG TCCCCCTGGA 
TTTTCAGGAA TTGATGGAGC AAGAGGACCT AAAGGAAACA AAGGTGACCC TGCCAGTCAC 
TTTGGTCCAC CTGGTCCAAA GGGTGAGCCA GGTAGCCCTG GATGTCCAGG GCATTTTGGA 
GCATCTGGAG AGCAGGGCTT GCCTGGTATT CAAGGGCCCA GAGGATCACC TGGAAGGCCA 
GGGCCACCTG GCTCCTCTGG ACCACCAGGG TGCCCAGGTG ATCACGGGAT GCCTGGGCTG 
AGGGGACAGC CAGGAGAAAT GGGAGACCCT GGGCCAAGAG GCCTCCAGGG GGATCCAGGG 
ATACCAGGTC CTCCGGGAAT AAAAGGTCCC TCCGGATCAC CTGGCCTGAA CGGCTTGCAT 
GGATTGAAAG GTCAGAAAGG AACTAAAGGT GCTTCAGGTT TGCATGATGT GGGGCCACCT 
GGTCCAGTGG GAATACCTGG GCTAAAAGGG GAGAGAGGAG ACCCTGGGAG CCCAGGAATC 
TCTCCTCCAG GTCCTCGTGG AGAAAAAGGT CCCCCAGGAC CCCCAGGGAG TTCAGGACCA 
CCTGGTCCTG CAGGTGCCAC AGGAAGAGCT CCTAAGGACA TTCCTGACCC GGGTCCACCT 
GGAGATCAGG GACCTCCTGG TCCTGATGGC CCAAGAGGAG CACCTGGGCC TCCAGGCCTC 
CCTGGGAGTG TTGACCTTCT GAGAGGGGAG CCAGGTGACT GTGGTCTACC AGGGCCACCA 
GGTCCCCCTG GCCCACCAGG CCCTCCAGGA TACAAAGGCT TTCCAGGATG TGATGGAAAA 
GATGGCCAGA AAGGACCAAT GGGATTCCCG GGACCGCAGG GACCACATGG ATTTCCTGGG 
CCACCTGGAG AGAAGGGTTT ACCTGGACCT CCAGGGAGAA AAGGGCCCAC TGGTCTTCCA 
GGTCCCAGAG GTGAACCGGG GCCACCTGCA GATGTGGATG ACTGTCCCCG AATCCCAGGC 
CTTCCTGGGG CGCCAGGCAT GAGAGGACCA GAAGGAGCCA TGGGGCTCTC TGGAATGAGA 
GGCCCCCCAG GACCAGGGTG CAAAGGAGAG CCTGGGCTGG ATGGCAGGAG GGGTGTGGAT 
GGCGTCCCTG GGTCTCCTGG GCCTCCCGGA CGTAAAGGTG ACACAGGAGA AGACGGCTAC 
CCTGGAGGAC CAGGGCCTCC TGGTCCCATT GGGGATCCTG GGCCCAAAGG GTTTGGCCCT 
GGATACCTCG GTGGCTTCCT CCTGGTTCTC CACAGTCAGA CGGACCAGGA GCCCACCTGC 
CCCCTGGGCA TGCCCAGGCT CTGGACTGGG TATAGTCTGT TATACCTGGA AGGGCAAGAG 
AAAGCTCACA ATCAAGACCT TGGTCTGGCA GGGTCTTGCC TTCCCGTGTT TAGCACGCTG 
CCCTTTGCCT ACTGCAACAT CCACCAGGTG TGCCACTATG CCCAGAGAAA CGACAGATCC 
TACTGGCTGG CCAGCGCTGC GCCCCTCCCC ATGATGCCAC TCTCTGAAGA GGCGATCCGC 
CCCTATGTCA GCCGCTGTGC GGTATGCGAG GCCCCGGCCC AGGCGGTGGC GGTGCACAGC 
CAGGACCAGT CCATCCCCCC ATGTCCGCAG ACCTGGAGGA GCCTCTGGAT CGGGTATTCA 
TTCCTGATGC TCATGGGTGT GTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL4A4 ( XM_016950593.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016806082.2 Pan troglodytes collagen type IV alpha 4 chain (COL4A4), transcript variant X10, mRNA.

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CloneID	OPc104274
Clone ID Related Accession (Same CDS sequence)	XM_009444469.3 , XM_009444470.3 , XM_016950593.2
Accession Version	XM_016950593.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4827bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	collagen alpha-4(IV) chain isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036881.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_016950593.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGTCTC TGCACATAGT ACTAATGAGG TGCTCCTTCA GATTGACCAA GTCCTTGGCC 
ACAGATCCCT GGTCACTTAC ACTCATTCTC TTTTCTGTAC AATATGTATA TGGGAGTGGA 
AAGAAATACG TTGGTCCTTG TGGAGGAAGA GATTGCTCTG TTTGCCACTG TGTTCCTGAA 
AAGGGGTCTC GGGGTCCACC AGGACCACCA GGGCCACAGG GTCCAATTGG ACCCCTGGGA 
GCCCCAGGAC CCATTGGGCT TTCAGGAGAG AAAGGAATGA GAGGGGACCG CGGCCCTCCT 
GGAGCAGCAG GGGACAAAGG AGATAAGGGT TCAACTGGTG TTCCTGGATT TCCAGGTTTA 
GATGGCATAC CTGGGCACCC AGGGCCTCCT GGACCCAGAG GCAAACCTGG TATGAGTGGC 
CACAATGGCT CAAGAGGTGA CCCAGGGTTT CCAGGAGGAA GAGGAGCTCT TGGCCCAGGA 
GGCCCCCTAG GCCATCCTGG GGAAAAGGGA GAAAAAGGAA ATTCAGTGTT CATTTTAGGT 
GCCGTTAAAG GTATTCAGGG AGACAGAGGG GACCCAGGAC TGCCTGGCTT ACCAGGATCT 
TGGGGGGCAG GAGGACCGGC GGGTCCCACA GGATATCCTG GAGAGCCAGG GTTAGTGGGA 
CCTCCGGGCC AACCAGGGCG TCCAGGTTTG AAGGGAAATC CCGGTGTGGG AGTAAAGGGG 
CAAATGGGAG ACCCGGGTGA GGTTGGTCAG CAAGGTTCTC CTGGACCCAC CCTGTTGGTG 
GAGCCACCTG ACTTTCGTCT CTATAAAGGA GAAAAGGGTA TAAAAGGAAT TCCTGGAATG 
GTTGGACTGC CAGGACCACC AGGACGCAAG GGAGAATCTG ATATTGGGGC AAAAGGAGAA 
AAAGGTATTC CTGGATTTCC AGGGCCTCGG GGGGATCCTG GTTCCTATGG ATCTCCAGGT 
TTTCCAGGAT TAAAGGGAGA ACTAGGACTG GTTGGAGATC CTGGGCTATT TGGATTAATT 
GGCCCAAAGG GGGATCCTGG AAATCGAGGG CACCCAGGAC CACCAGGTGT TTTGGTGACT 
CCACCTCTTC CACTCAAAGG CCCACCAGGG GACCCAGGGT TCCCTGGCCG CTATGGAGAA 
ACAGGGGATG TTGGACCACC TGGTCCCCCA GGTCTCTTGG GCAGACCAGG GGAAGCCTGT 
GCAGGCATGA TAGGACCCCC TGGGCCACAA GGATTTCCTG GTCTTCCTGG GCTTCCAGGA 
GAAGCTGGTA TTCCTGGGAG ACCTGATTCT GCTCCAGGAA AACCAGGGAA GCCAGGATCA 
CCTGGCTTGC CTGGAGCACC AGGCCTGCAG GGCCTCCCAG GATCAAGTGT GATATACTGT 
AGTGTTGGGA ATCCTGGACC ACAAGGAATA AAAGGCAAAG TGGGTCCCCC AGGAGGAAGA 
GGCTCAAAAG GAGAAAAAGG AAATGAAGGA CTCTGTGCCT GTGAGCCTGG ACCCATGGGC 
CCCCCTGGCC CTCCAGGACT TCCTGGGAGG CAGGGGAGTA AGGGAGACTT GGGGCTCCCT 
GGCTGGCTTG GAACAAAAGG TGACCCAGGA CCTCCTGGTG CTGAAGGACC TCCAGGGCTA 
CCAGGAAAGC ATGGTGCCTC TGGACCACCT GGCAACAAAG GGGCGAAGGG TGACATGGTT 
GTATCAAGAG TTAAAGGGCA CAAAGGAGAA AGAGGTCCTG ATGGGCCCCC AGGATTTCCA 
GGGCAGCCAG GATCACATGG TCGGGATGGA CATGCTGGAG AAAAAGGGGA TCCAGGACCC 
CCAGGGGATC ATGAAGATGC GACCCCAGGT GGTAAAGGAT TTCCTGGACC TCTGGGCCCC 
CCGGGCAAAG CAGGACCTTT GGGGCCCCCA GGACTGGGAT TTCCTGGTCC ACCAGGACAG 
CGAGGCCACC CAGGAGTTCC AGGCCACCCA GGTGTGAGGG GCCCTGATGG CTTGAAGGGT 
CAGAAAGGTG ACACAATTTC TTGCAACGTA ACCTACCCTG GGAGGCACGG CCCTCCAGGT 
TTTGATGGAC CTCCAGGTCC GAAGGGATTT CCAGGTCCCC AAGGTGCCCC TGGGCTGAGT 
GGTTCAGATG GGCATAAAGG CAGACCTGGC ACACCAGGAA CAGCGGAAAT ACCAGGTCCA 
CCTGGTTTTC GTGGTGACAT GGGAGATCCG GGTTTTGGAG GTGAAAAGGG GTCCTCCCCT 
GCTGGGCCCC CAGGCCCTCC CGGCTCACCA GGAGTGAATG GTCAGAAAGG AATCCCGGGA 
GACCCTGCAT TTGGTCACCT GGGACCCCCG GGAAAGAGGG GTCTTTCAGG AGTGCCAGGG 
ATAAAAGGAC CCAGAGGTGA TCCGGGATGT CCAGGGGCTG AAGGGCCAGC TGGCATTCCT 
GGATTCCCAG GTCTCAAAGG TCCCAAAGGC AGAGAGGGAC ATGCTGGGTT TCCAGGTGTC 
CCAGGTCCAC CTGGCCATTC CTGTGAAAGA GGTGCTCCAG GGATACCAGG GCAACCGGGA 
CTCCCTGGGT ATCCAGGTAG CCCAGGTGCT CCAGGTGGGA AAGGACAGCT GGGAGATGTG 
GGGCCTCCCG GGCCAGCTGG AATGAAAGGC CTCCCTGGAC TCCCAGGATG GCCTGGGGCA 
CATGGTCCCC CAGGCCTCCC AGGAATCCCA GGTCCCTTTG GAGATGATGG GCTACCTGGT 
CCTCCAGGTC CAAAGGGACC CCAGGGGCTG CCTGGTTTCC CAGGTTTTCC CGGAGAAAGA 
GGAAAGCCTG GTGCAGAGGG ATGTCCTGGC ACAAAGGGAG AACCTGGAGA GAAGGGCATG 
TCTGGCCTTC CCGGAGACCG GGGACTGAGA GGGGCCAAAG GAGCCATAGG ACCTCCCGGA 
GATGAAGGAG AAATGGCTAT CATTTCCCAA AAGGGAACAC CTGGGGAACC TGGACCTCCT 
GGAGATGATG GATTCCCAGG AGAAAGAGGT GATAAAGGAA CTCCCGGGAT GCAAGGGAGA 
AGAGGAGAGC CGGGAAGATA CGGACCACCT GGATTTCAGA GAGGGGAACC TGGTGAGAAA 
GGTCAGCCAG GGCATCCTGG ACCCCCAGGC CCTCCAGGCT CAACTGGTCT AAGAGGGTTC 
ATTGGTTTTC CAGGACTTCC AGGTGACCAG GGTGAGCCAG GTTCTCCAGG TCCCCCTGGA 
TTTTCAGGAA TTGATGGAGC AAGAGGACCT AAAGGAAACA AAGGTGACCC TGCCAGTCAC 
TTTGGTCCAC CTGGTCCAAA GGGTGAGCCA GGTAGCCCTG GATGTCCAGG GCATTTTGGA 
GCATCTGGAG AGCAGGGCTT GCCTGGTATT CAAGGGCCCA GAGGATCACC TGGAAGGCCA 
GGGCCACCTG GCTCCTCTGG ACCACCAGGG TGCCCAGGTG ATCACGGGAT GCCTGGGCTG 
AGGGGACAGC CAGGAGAAAT GGGAGACCCT GGGCCAAGAG GCCTCCAGGG GGATCCAGGG 
ATACCAGGTC CTCCGGGAAT AAAAGGTCCC TCCGGATCAC CTGGCCTGAA CGGCTTGCAT 
GGATTGAAAG GTCAGAAAGG AACTAAAGGT GCTTCAGGTT TGCATGATGT GGGGCCACCT 
GGTCCAGTGG GAATACCTGG GCTAAAAGGG GAGAGAGGAG ACCCTGGGAG CCCAGGAATC 
TCTCCTCCAG GTCCTCGTGG AGAAAAAGGT CCCCCAGGAC CCCCAGGGAG TTCAGGACCA 
CCTGGTCCTG CAGGTGCCAC AGGAAGAGCT CCTAAGGACA TTCCTGACCC GGGTCCACCT 
GGAGATCAGG GACCTCCTGG TCCTGATGGC CCAAGAGGAG CACCTGGGCC TCCAGGCCTC 
CCTGGGAGTG TTGACCTTCT GAGAGGGGAG CCAGGTGACT GTGGTCTACC AGGGCCACCA 
GGTCCCCCTG GCCCACCAGG CCCTCCAGGA TACAAAGGCT TTCCAGGATG TGATGGAAAA 
GATGGCCAGA AAGGACCAAT GGGATTCCCG GGACCGCAGG GACCACATGG ATTTCCTGGG 
CCACCTGGAG AGAAGGGTTT ACCTGGACCT CCAGGGAGAA AAGGGCCCAC TGGTCTTCCA 
GGTCCCAGAG GTGAACCGGG GCCACCTGCA GATGTGGATG ACTGTCCCCG AATCCCAGGC 
CTTCCTGGGG CGCCAGGCAT GAGAGGACCA GAAGGAGCCA TGGGGCTCTC TGGAATGAGA 
GGCCCCCCAG GACCAGGGTG CAAAGGAGAG CCTGGGCTGG ATGGCAGGAG GGGTGTGGAT 
GGCGTCCCTG GGTCTCCTGG GCCTCCCGGA CGTAAAGGTG ACACAGGAGA AGACGGCTAC 
CCTGGAGGAC CAGGGCCTCC TGGTCCCATT GGGGATCCTG GGCCCAAAGG GTTTGGCCCT 
GGATACCTCG GTGGCTTCCT CCTGGTTCTC CACAGTCAGA CGGACCAGGA GCCCACCTGC 
CCCCTGGGCA TGCCCAGGCT CTGGACTGGG TATAGTCTGT TATACCTGGA AGGGCAAGAG 
AAAGCTCACA ATCAAGACCT TGGTCTGGCA GGGTCTTGCC TTCCCGTGTT TAGCACGCTG 
CCCTTTGCCT ACTGCAACAT CCACCAGGTG TGCCACTATG CCCAGAGAAA CGACAGATCC 
TACTGGCTGG CCAGCGCTGC GCCCCTCCCC ATGATGCCAC TCTCTGAAGA GGCGATCCGC 
CCCTATGTCA GCCGCTGTGC GGTATGCGAG GCCCCGGCCC AGGCGGTGGC GGTGCACAGC 
CAGGACCAGT CCATCCCCCC ATGTCCGCAG ACCTGGAGGA GCCTCTGGAT CGGGTATTCA 
TTCCTGATGC TCATGGGTGT GTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016806082.2
CDS	423..5249
Translation	MWSLHIVLMRCSFRLTKSLATDPWSLTLILFSVQYVYGSGKKYVGPCGGRDCSVCHCVPEKGSRGPPGPPGPQGPIGPLGAPGPIGLSGEKGMRGDRGPPGAAGDKGDKGSTGVPGFPGLDGIPGHPGPPGPRGKPGMSGHNGSRGDPGFPGGRGALGPGGPLGHPGEKGEKGNSVFILGAVKGIQGDRGDPGLPGLPGSWGAGGPAGPTGYPGEPGLVGPPGQPGRPGLKGNPGVGVKGQMGDPGEVGQQGSPGPTLLVEPPDFRLYKGEKGIKGIPGMVGLPGPPGRKGESDIGAKGEKGIPGFPGPRGDPGSYGSPGFPGLKGELGLVGDPGLFGLIGPKGDPGNRGHPGPPGVLVTPPLPLKGPPGDPGFPGRYGETGDVGPPGPPGLLGRPGEACAGMIGPPGPQGFPGLPGLPGEAGIPGRPDSAPGKPGKPGSPGLPGAPGLQGLPGSSVIYCSVGNPGPQGIKGKVGPPGGRGSKGEKGNEGLCACEPGPMGPPGPPGLPGRQGSKGDLGLPGWLGTKGDPGPPGAEGPPGLPGKHGASGPPGNKGAKGDMVVSRVKGHKGERGPDGPPGFPGQPGSHGRDGHAGEKGDPGPPGDHEDATPGGKGFPGPLGPPGKAGPLGPPGLGFPGPPGQRGHPGVPGHPGVRGPDGLKGQKGDTISCNVTYPGRHGPPGFDGPPGPKGFPGPQGAPGLSGSDGHKGRPGTPGTAEIPGPPGFRGDMGDPGFGGEKGSSPAGPPGPPGSPGVNGQKGIPGDPAFGHLGPPGKRGLSGVPGIKGPRGDPGCPGAEGPAGIPGFPGLKGPKGREGHAGFPGVPGPPGHSCERGAPGIPGQPGLPGYPGSPGAPGGKGQLGDVGPPGPAGMKGLPGLPGWPGAHGPPGLPGIPGPFGDDGLPGPPGPKGPQGLPGFPGFPGERGKPGAEGCPGTKGEPGEKGMSGLPGDRGLRGAKGAIGPPGDEGEMAIISQKGTPGEPGPPGDDGFPGERGDKGTPGMQGRRGEPGRYGPPGFQRGEPGEKGQPGHPGPPGPPGSTGLRGFIGFPGLPGDQGEPGSPGPPGFSGIDGARGPKGNKGDPASHFGPPGPKGEPGSPGCPGHFGASGEQGLPGIQGPRGSPGRPGPPGSSGPPGCPGDHGMPGLRGQPGEMGDPGPRGLQGDPGIPGPPGIKGPSGSPGLNGLHGLKGQKGTKGASGLHDVGPPGPVGIPGLKGERGDPGSPGISPPGPRGEKGPPGPPGSSGPPGPAGATGRAPKDIPDPGPPGDQGPPGPDGPRGAPGPPGLPGSVDLLRGEPGDCGLPGPPGPPGPPGPPGYKGFPGCDGKDGQKGPMGFPGPQGPHGFPGPPGEKGLPGPPGRKGPTGLPGPRGEPGPPADVDDCPRIPGLPGAPGMRGPEGAMGLSGMRGPPGPGCKGEPGLDGRRGVDGVPGSPGPPGRKGDTGEDGYPGGPGPPGPIGDPGPKGFGPGYLGGFLLVLHSQTDQEPTCPLGMPRLWTGYSLLYLEGQEKAHNQDLGLAGSCLPVFSTLPFAYCNIHQVCHYAQRNDRSYWLASAAPLPMMPLSEEAIRPYVSRCAVCEAPAQAVAVHSQDQSIPPCPQTWRSLWIGYSFLMLMGVF

Target ORF information:

RefSeq Version	XM_016950593.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes collagen type IV alpha 4 chain (COL4A4), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016950593.2

ORF Insert Sequence:

ATGTGGTCTC TGCACATAGT ACTAATGAGG TGCTCCTTCA GATTGACCAA GTCCTTGGCC 
ACAGATCCCT GGTCACTTAC ACTCATTCTC TTTTCTGTAC AATATGTATA TGGGAGTGGA 
AAGAAATACG TTGGTCCTTG TGGAGGAAGA GATTGCTCTG TTTGCCACTG TGTTCCTGAA 
AAGGGGTCTC GGGGTCCACC AGGACCACCA GGGCCACAGG GTCCAATTGG ACCCCTGGGA 
GCCCCAGGAC CCATTGGGCT TTCAGGAGAG AAAGGAATGA GAGGGGACCG CGGCCCTCCT 
GGAGCAGCAG GGGACAAAGG AGATAAGGGT TCAACTGGTG TTCCTGGATT TCCAGGTTTA 
GATGGCATAC CTGGGCACCC AGGGCCTCCT GGACCCAGAG GCAAACCTGG TATGAGTGGC 
CACAATGGCT CAAGAGGTGA CCCAGGGTTT CCAGGAGGAA GAGGAGCTCT TGGCCCAGGA 
GGCCCCCTAG GCCATCCTGG GGAAAAGGGA GAAAAAGGAA ATTCAGTGTT CATTTTAGGT 
GCCGTTAAAG GTATTCAGGG AGACAGAGGG GACCCAGGAC TGCCTGGCTT ACCAGGATCT 
TGGGGGGCAG GAGGACCGGC GGGTCCCACA GGATATCCTG GAGAGCCAGG GTTAGTGGGA 
CCTCCGGGCC AACCAGGGCG TCCAGGTTTG AAGGGAAATC CCGGTGTGGG AGTAAAGGGG 
CAAATGGGAG ACCCGGGTGA GGTTGGTCAG CAAGGTTCTC CTGGACCCAC CCTGTTGGTG 
GAGCCACCTG ACTTTCGTCT CTATAAAGGA GAAAAGGGTA TAAAAGGAAT TCCTGGAATG 
GTTGGACTGC CAGGACCACC AGGACGCAAG GGAGAATCTG ATATTGGGGC AAAAGGAGAA 
AAAGGTATTC CTGGATTTCC AGGGCCTCGG GGGGATCCTG GTTCCTATGG ATCTCCAGGT 
TTTCCAGGAT TAAAGGGAGA ACTAGGACTG GTTGGAGATC CTGGGCTATT TGGATTAATT 
GGCCCAAAGG GGGATCCTGG AAATCGAGGG CACCCAGGAC CACCAGGTGT TTTGGTGACT 
CCACCTCTTC CACTCAAAGG CCCACCAGGG GACCCAGGGT TCCCTGGCCG CTATGGAGAA 
ACAGGGGATG TTGGACCACC TGGTCCCCCA GGTCTCTTGG GCAGACCAGG GGAAGCCTGT 
GCAGGCATGA TAGGACCCCC TGGGCCACAA GGATTTCCTG GTCTTCCTGG GCTTCCAGGA 
GAAGCTGGTA TTCCTGGGAG ACCTGATTCT GCTCCAGGAA AACCAGGGAA GCCAGGATCA 
CCTGGCTTGC CTGGAGCACC AGGCCTGCAG GGCCTCCCAG GATCAAGTGT GATATACTGT 
AGTGTTGGGA ATCCTGGACC ACAAGGAATA AAAGGCAAAG TGGGTCCCCC AGGAGGAAGA 
GGCTCAAAAG GAGAAAAAGG AAATGAAGGA CTCTGTGCCT GTGAGCCTGG ACCCATGGGC 
CCCCCTGGCC CTCCAGGACT TCCTGGGAGG CAGGGGAGTA AGGGAGACTT GGGGCTCCCT 
GGCTGGCTTG GAACAAAAGG TGACCCAGGA CCTCCTGGTG CTGAAGGACC TCCAGGGCTA 
CCAGGAAAGC ATGGTGCCTC TGGACCACCT GGCAACAAAG GGGCGAAGGG TGACATGGTT 
GTATCAAGAG TTAAAGGGCA CAAAGGAGAA AGAGGTCCTG ATGGGCCCCC AGGATTTCCA 
GGGCAGCCAG GATCACATGG TCGGGATGGA CATGCTGGAG AAAAAGGGGA TCCAGGACCC 
CCAGGGGATC ATGAAGATGC GACCCCAGGT GGTAAAGGAT TTCCTGGACC TCTGGGCCCC 
CCGGGCAAAG CAGGACCTTT GGGGCCCCCA GGACTGGGAT TTCCTGGTCC ACCAGGACAG 
CGAGGCCACC CAGGAGTTCC AGGCCACCCA GGTGTGAGGG GCCCTGATGG CTTGAAGGGT 
CAGAAAGGTG ACACAATTTC TTGCAACGTA ACCTACCCTG GGAGGCACGG CCCTCCAGGT 
TTTGATGGAC CTCCAGGTCC GAAGGGATTT CCAGGTCCCC AAGGTGCCCC TGGGCTGAGT 
GGTTCAGATG GGCATAAAGG CAGACCTGGC ACACCAGGAA CAGCGGAAAT ACCAGGTCCA 
CCTGGTTTTC GTGGTGACAT GGGAGATCCG GGTTTTGGAG GTGAAAAGGG GTCCTCCCCT 
GCTGGGCCCC CAGGCCCTCC CGGCTCACCA GGAGTGAATG GTCAGAAAGG AATCCCGGGA 
GACCCTGCAT TTGGTCACCT GGGACCCCCG GGAAAGAGGG GTCTTTCAGG AGTGCCAGGG 
ATAAAAGGAC CCAGAGGTGA TCCGGGATGT CCAGGGGCTG AAGGGCCAGC TGGCATTCCT 
GGATTCCCAG GTCTCAAAGG TCCCAAAGGC AGAGAGGGAC ATGCTGGGTT TCCAGGTGTC 
CCAGGTCCAC CTGGCCATTC CTGTGAAAGA GGTGCTCCAG GGATACCAGG GCAACCGGGA 
CTCCCTGGGT ATCCAGGTAG CCCAGGTGCT CCAGGTGGGA AAGGACAGCT GGGAGATGTG 
GGGCCTCCCG GGCCAGCTGG AATGAAAGGC CTCCCTGGAC TCCCAGGATG GCCTGGGGCA 
CATGGTCCCC CAGGCCTCCC AGGAATCCCA GGTCCCTTTG GAGATGATGG GCTACCTGGT 
CCTCCAGGTC CAAAGGGACC CCAGGGGCTG CCTGGTTTCC CAGGTTTTCC CGGAGAAAGA 
GGAAAGCCTG GTGCAGAGGG ATGTCCTGGC ACAAAGGGAG AACCTGGAGA GAAGGGCATG 
TCTGGCCTTC CCGGAGACCG GGGACTGAGA GGGGCCAAAG GAGCCATAGG ACCTCCCGGA 
GATGAAGGAG AAATGGCTAT CATTTCCCAA AAGGGAACAC CTGGGGAACC TGGACCTCCT 
GGAGATGATG GATTCCCAGG AGAAAGAGGT GATAAAGGAA CTCCCGGGAT GCAAGGGAGA 
AGAGGAGAGC CGGGAAGATA CGGACCACCT GGATTTCAGA GAGGGGAACC TGGTGAGAAA 
GGTCAGCCAG GGCATCCTGG ACCCCCAGGC CCTCCAGGCT CAACTGGTCT AAGAGGGTTC 
ATTGGTTTTC CAGGACTTCC AGGTGACCAG GGTGAGCCAG GTTCTCCAGG TCCCCCTGGA 
TTTTCAGGAA TTGATGGAGC AAGAGGACCT AAAGGAAACA AAGGTGACCC TGCCAGTCAC 
TTTGGTCCAC CTGGTCCAAA GGGTGAGCCA GGTAGCCCTG GATGTCCAGG GCATTTTGGA 
GCATCTGGAG AGCAGGGCTT GCCTGGTATT CAAGGGCCCA GAGGATCACC TGGAAGGCCA 
GGGCCACCTG GCTCCTCTGG ACCACCAGGG TGCCCAGGTG ATCACGGGAT GCCTGGGCTG 
AGGGGACAGC CAGGAGAAAT GGGAGACCCT GGGCCAAGAG GCCTCCAGGG GGATCCAGGG 
ATACCAGGTC CTCCGGGAAT AAAAGGTCCC TCCGGATCAC CTGGCCTGAA CGGCTTGCAT 
GGATTGAAAG GTCAGAAAGG AACTAAAGGT GCTTCAGGTT TGCATGATGT GGGGCCACCT 
GGTCCAGTGG GAATACCTGG GCTAAAAGGG GAGAGAGGAG ACCCTGGGAG CCCAGGAATC 
TCTCCTCCAG GTCCTCGTGG AGAAAAAGGT CCCCCAGGAC CCCCAGGGAG TTCAGGACCA 
CCTGGTCCTG CAGGTGCCAC AGGAAGAGCT CCTAAGGACA TTCCTGACCC GGGTCCACCT 
GGAGATCAGG GACCTCCTGG TCCTGATGGC CCAAGAGGAG CACCTGGGCC TCCAGGCCTC 
CCTGGGAGTG TTGACCTTCT GAGAGGGGAG CCAGGTGACT GTGGTCTACC AGGGCCACCA 
GGTCCCCCTG GCCCACCAGG CCCTCCAGGA TACAAAGGCT TTCCAGGATG TGATGGAAAA 
GATGGCCAGA AAGGACCAAT GGGATTCCCG GGACCGCAGG GACCACATGG ATTTCCTGGG 
CCACCTGGAG AGAAGGGTTT ACCTGGACCT CCAGGGAGAA AAGGGCCCAC TGGTCTTCCA 
GGTCCCAGAG GTGAACCGGG GCCACCTGCA GATGTGGATG ACTGTCCCCG AATCCCAGGC 
CTTCCTGGGG CGCCAGGCAT GAGAGGACCA GAAGGAGCCA TGGGGCTCTC TGGAATGAGA 
GGCCCCCCAG GACCAGGGTG CAAAGGAGAG CCTGGGCTGG ATGGCAGGAG GGGTGTGGAT 
GGCGTCCCTG GGTCTCCTGG GCCTCCCGGA CGTAAAGGTG ACACAGGAGA AGACGGCTAC 
CCTGGAGGAC CAGGGCCTCC TGGTCCCATT GGGGATCCTG GGCCCAAAGG GTTTGGCCCT 
GGATACCTCG GTGGCTTCCT CCTGGTTCTC CACAGTCAGA CGGACCAGGA GCCCACCTGC 
CCCCTGGGCA TGCCCAGGCT CTGGACTGGG TATAGTCTGT TATACCTGGA AGGGCAAGAG 
AAAGCTCACA ATCAAGACCT TGGTCTGGCA GGGTCTTGCC TTCCCGTGTT TAGCACGCTG 
CCCTTTGCCT ACTGCAACAT CCACCAGGTG TGCCACTATG CCCAGAGAAA CGACAGATCC 
TACTGGCTGG CCAGCGCTGC GCCCCTCCCC ATGATGCCAC TCTCTGAAGA GGCGATCCGC 
CCCTATGTCA GCCGCTGTGC GGTATGCGAG GCCCCGGCCC AGGCGGTGGC GGTGCACAGC 
CAGGACCAGT CCATCCCCCC ATGTCCGCAG ACCTGGAGGA GCCTCTGGAT CGGGTATTCA 
TTCCTGATGC TCATGGGTGT GTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL4A4 ( XM_009444471.3 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_009442746.3 Pan troglodytes collagen type IV alpha 4 chain (COL4A4), transcript variant X12, mRNA.

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CloneID	OPc104275
Clone ID Related Accession (Same CDS sequence)	XM_009444471.3
Accession Version	XM_009444471.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4821bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	collagen alpha-4(IV) chain isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036881.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_009444471.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGTCTC TGCACATAGT ACTAATGAGG TGCTCCTTCA GATTGACCAA GTCCTTGGCC 
ACAGATCCCT GGTCACTTAC ACTCATTCTC TTTTCTGTAC AATATGTATA TGGGAGTGGA 
AAGAAATACG TTGGTCCTTG TGGAGGAAGA GATTGCTCTG TTTGCCACTG TGTTCCTGAA 
AAGGGGTCTC GGGGTCCACC AGGACCACCA GGGCCACAGG GTCCAATTGG ACCCCTGGGA 
GCCCCAGGAC CCATTGGGCT TTCAGGAGAG AAAGGAATGA GAGGGGACCG CGGCCCTCCT 
GGAGCAGCAG GGGACAAAGG AGATAAGGGT TCAACTGGTG TTCCTGGATT TCCAGGTTTA 
GATGGCATAC CTGGGCACCC AGGGCCTCCT GGACCCAGAG GCAAACCTGG TATGAGTGGC 
CACAATGGCT CAAGAGGTGA CCCAGGGTTT CCAGGAGGAA GAGGAGCTCT TGGCCCAGGA 
GGCCCCCTAG GCCATCCTGG GGAAAAGGGA GAAAAAGGAA ATTCAGTGTT CATTTTAGGT 
GCCGTTAAAG GTATTCAGGG AGACAGAGGG GACCCAGGAC TGCCTGGCTT ACCAGGATCT 
TGGGGGGCAG GAGGACCGGC GGGTCCCACA GGATATCCTG GAGAGCCAGG GTTAGTGGGA 
CCTCCGGGCC AACCAGGGCG TCCAGGTTTG AAGGGAAATC CCGGTGTGGG AGTAAAGGGG 
CAAATGGGAG ACCCGGGTGA GGTTGGTCAG CAAGGTTCTC CTGGACCCAC CCTGTTGGTG 
GAGCCACCTG ACTTTCGTCT CTATAAAGGA GAAAAGGGTA TAAAAGGAAT TCCTGGAATG 
GTTGGACTGC CAGGACCACC AGGACGCAAG GGAGAATCTG ATATTGGGGC AAAAGGAGAA 
AAAGGTATTC CTGGATTTCC AGGGCCTCGG GGGGATCCTG GTTCCTATGG ATCTCCAGGT 
TTTCCAGGAT TAAAGGGAGA ACTAGGACTG GTTGGAGATC CTGGGCTATT TGGATTAATT 
GGCCCAAAGG GGGATCCTGG AAATCGAGGG CACCCAGGAC CACCAGGTGT TTTGGTGACT 
CCACCTCTTC CACTCAAAGG CCCACCAGGG GACCCAGGGT TCCCTGGCCG CTATGGAGAA 
ACAGGGGATG TTGGACCACC TGGTCCCCCA GGTCTCTTGG GCAGACCAGG GGAAGCCTGT 
GCAGGCATGA TAGGACCCCC TGGGCCACAA GGATTTCCTG GTCTTCCTGG GCTTCCAGGA 
GAAGCTGGTA TTCCTGGGAG ACCTGATTCT GCTCCAGGAA AACCAGGGAA GCCAGGATCA 
CCTGGCTTGC CTGGAGCACC AGGCCTGCAG GGCCTCCCAG GATCAAGTGT GATATACTGT 
AGTGTTGGGA ATCCTGGACC ACAAGGAATA AAAGGCAAAG TGGGTCCCCC AGGAGGAAGA 
GGCTCAAAAG GAGAAAAAGG AAATGAAGGA CTCTGTGCCT GTGAGCCTGG ACCCATGGGC 
CCCCCTGGCC CTCCAGGACT TCCTGGGAGG CAGGGGAGTA AGGGAGACTT GGGGCTCCCT 
GGCTGGCTTG GAACAAAAGG TGACCCAGGA CCTCCTGGTG CTGAAGGACC TCCAGGGCTA 
CCAGGAAAGC ATGGTGCCTC TGGACCACCT GGCAACAAAG GGGCGAAGGG TGACATGGTT 
GTATCAAGAG TTAAAGGGCA CAAAGGAGAA AGAGGTCCTG ATGGGCCCCC AGGATTTCCA 
GGGCAGCCAG GATCACATGG TCGGGATGGA CATGCTGGAG AAAAAGGGGA TCCAGGACCC 
CCAGGGGATC ATGAAGATGC GACCCCAGGT GGTAAAGGAT TTCCTGGACC TCTGGGCCCC 
CCGGGCAAAG CAGGACCTTT GGGGCCCCCA GGACTGGGAT TTCCTGGTCC ACCAGGACAG 
CGAGGCCACC CAGGAGTTCC AGGCCACCCA GGTGTGAGGG GCCCTGATGG CTTGAAGGGT 
CAGAAAGGTG ACACAATTTC TTGCAACGTA ACCTACCCTG GGAGGCACGG CCCTCCAGGT 
TTTGATGGAC CTCCAGGTCC GAAGGGATTT CCAGGTCCCC AAGGTGCCCC TGGGCTGAGT 
GGTTCAGATG GGCATAAAGG CAGACCTGGC ACACCAGGAA CAGCGGAAAT ACCAGGTCCA 
CCTGGTTTTC GTGGTGACAT GGGAGATCCG GGTTTTGGAG GTGAAAAGGG GTCCTCCCCT 
GCTGGGCCCC CAGGCCCTCC CGGCTCACCA GGAGTGAATG GTCAGAAAGG AATCCCGGGA 
GACCCTGCAT TTGGTCACCT GGGACCCCCG GGAAAGAGGG GTCTTTCAGG AGTGCCAGGG 
ATAAAAGGAC CCAGAGGTGA TCCGGGATGT CCAGGGGCTG AAGGGCCAGC TGGCATTCCT 
GGATTCCCAG GTCTCAAAGG TCCCAAAGGC AGAGAGGGAC ATGCTGGGTT TCCAGGTGTC 
CCAGGTCCAC CTGGCCATTC CTGTGAAAGA GGTGCTCCAG GGATACCAGG GCAACCGGGA 
CTCCCTGGGT ATCCAGGTAG CCCAGGTGCT CCAGGTGGGA AAGGACAGCT GGGAGATGTG 
GGGCCTCCCG GGCCAGCTGG AATGAAAGGC CTCCCTGGAC TCCCAGGATG GCCTGGGGCA 
CATGGTCCCC CAGGCCTCCC AGGAATCCCA GGTCCCTTTG GAGATGATGG GCTACCTGGT 
CCTCCAGGTC CAAAGGGACC CCAGGGGCTG CCTGGTTTCC CAGGTTTTCC CGGAGAAAGA 
GGAAAGCCTG GTGCAGAGGG ATGTCCTGGC ACAAAGGGAG AACCTGGAGA GAAGGGCATG 
TCTGGCCTTC CCGGAGACCG GGGACTGAGA GGGGCCAAAG GAGCCATAGG ACCTCCCGGA 
GATGAAGGAG AAATGGCTAT CATTTCCCAA AAGGGAACAC CTGGGGAACC TGGACCTCCT 
GGAGATGATG GATTCCCAGG AGAAAGAGGT GATAAAGGAA CTCCCGGGAT GCAAGGGAGA 
AGAGGAGAGC CGGGAAGATA CGGACCACCT GGATTTCAGA GAGGGGAACC TGGTGAGAAA 
GGTCAGCCAG GGCATCCTGG ACCCCCAGGC CCTCCAGGCT CAACTGGTCT AAGAGGGTTC 
ATTGGTTTTC CAGGACTTCC AGGTGACCAG GGTGAGCCAG GTTCTCCAGG TCCCCCTGGA 
TTTTCAGGAA TTGATGGAGC AAGAGGACCT AAAGGAAACA AAGGTGACCC TGCCAGTCAC 
TTTGGTCCAC CTGGTCCAAA GGGTGAGCCA GGTAGCCCTG GATGTCCAGG GCATTTTGGA 
GCATCTGGAG AGCAGGGCTT GCCTGGTATT CAAGGGCCCA GAGGATCACC TGGAAGGCCA 
GGGCCACCTG GCTCCTCTGG ACCACCAGGG TGCCCAGGTG ATCACGGGAT GCCTGGGCTG 
AGGGGACAGC CAGGAGAAAT GGGAGACCCT GGGCCAAGAG GCCTCCAGGG GGATCCAGGG 
ATACCAGGTC CTCCGGGAAT AAAAGGTCCC TCCGGATCAC CTGGCCTGAA CGGCTTGCAT 
GGATTGAAAG GTCAGAAAGG AACTAAAGGT GCTTCAGGTT TGCATGATGT GGGGCCACCT 
GGTCCAGTGG GAATACCTGG GCTAAAAGGG GAGAGAGGAG ACCCTGGGAG CCCAGGAATC 
TCTCCTCCAG GTCCTCGTGG AGAAAAAGGT CCCCCAGGAC CCCCAGGGAG TTCAGGACCA 
CCTGGTCCTG CAGGTGCCAC AGGAAGAGCT CCTAAGGACA TTCCTGACCC GGGTCCACCT 
GGAGATCAGG GACCTCCTGG TCCTGATGGC CCAAGAGGAG CACCTGGGCC TCCAGGCCTC 
CCTGGGAGTG TTGACCTTCT GAGAGGGGAG CCAGGTGACT GTGGTCTACC AGGGCCACCA 
GGTCCCCCTG GCCCACCAGG CCCTCCAGGA TACAAAGGCT TTCCAGGATG TGATGGAAAA 
GATGGCCAGA AAGGACCAAT GGGATTCCCG GGACCGCAGG GACCACATGG ATTTCCTGGG 
CCACCTGGAG AGAAGGGTTT ACCTGGACCT CCAGGGAGAA AAGGGCCCAC TGGTCTTCCA 
GGTCCCAGAG GTGAACCGGG GCCACCTGCA GATGTGGATG ACTGTCCCCG AATCCCAGGC 
CTTCCTGGGG CGCCAGGCAT GAGAGGACCA GAAGGAGCCA TGGGGCTCTC TGGAATGAGA 
GGCCCCCCAG GACCAGGGTG CAAAGGAGAG CCTGGGCTGG ATGGCAGGAG GGGTGTGGAT 
GGCGTCCCTG GGTCTCCTGG GCCTCCCGGA CGTAAAGGTG ACACAGGAGA AGACGGCTAC 
CCTGGAGGAC CAGGGCCTCC TGGTCCCATT GGGGATCCTG GGCCCAAAGG GTTTGGCCCT 
GGATACCTCG GTGGCTTCCT CCTGGTTCTC CACAGTCAGA CGGACCAGGA GCCCACCTGC 
CCCCTGGGCA TGCCCAGGCT CTGGACTGGG TATAGTCTGT TATACCTGGA AGGGCAAGAG 
AAAGCTCACA ATCAAGACCT TGGTCTGGCA GGGTCTTGCC TTCCCGTGTT TAGCACGCTG 
CCCTTTGCCT ACTGCAACAT CCACCAGGTG TGCCACTATG CCCAGAGAAA CGACAGATCC 
TACTGGCTGG CCAGCGCTGC GCCCCTCCCC ATGATGCCAC TCTCTGAAGA GGCGATCCGC 
CCCTATGTCA GCCGCTGTGC GGTATGCGAG GCCCCGGCCC AGGCGGTGGC GGTGCACAGC 
CAGGACCAGT CCATCCCCCC ATGTCCGCAG ACCTGGAGGA GCCTCTGGAT CGGGTATTCA 
TTCCTGATGC TTACATTGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009442746.3
CDS	423..5243
Translation	MWSLHIVLMRCSFRLTKSLATDPWSLTLILFSVQYVYGSGKKYVGPCGGRDCSVCHCVPEKGSRGPPGPPGPQGPIGPLGAPGPIGLSGEKGMRGDRGPPGAAGDKGDKGSTGVPGFPGLDGIPGHPGPPGPRGKPGMSGHNGSRGDPGFPGGRGALGPGGPLGHPGEKGEKGNSVFILGAVKGIQGDRGDPGLPGLPGSWGAGGPAGPTGYPGEPGLVGPPGQPGRPGLKGNPGVGVKGQMGDPGEVGQQGSPGPTLLVEPPDFRLYKGEKGIKGIPGMVGLPGPPGRKGESDIGAKGEKGIPGFPGPRGDPGSYGSPGFPGLKGELGLVGDPGLFGLIGPKGDPGNRGHPGPPGVLVTPPLPLKGPPGDPGFPGRYGETGDVGPPGPPGLLGRPGEACAGMIGPPGPQGFPGLPGLPGEAGIPGRPDSAPGKPGKPGSPGLPGAPGLQGLPGSSVIYCSVGNPGPQGIKGKVGPPGGRGSKGEKGNEGLCACEPGPMGPPGPPGLPGRQGSKGDLGLPGWLGTKGDPGPPGAEGPPGLPGKHGASGPPGNKGAKGDMVVSRVKGHKGERGPDGPPGFPGQPGSHGRDGHAGEKGDPGPPGDHEDATPGGKGFPGPLGPPGKAGPLGPPGLGFPGPPGQRGHPGVPGHPGVRGPDGLKGQKGDTISCNVTYPGRHGPPGFDGPPGPKGFPGPQGAPGLSGSDGHKGRPGTPGTAEIPGPPGFRGDMGDPGFGGEKGSSPAGPPGPPGSPGVNGQKGIPGDPAFGHLGPPGKRGLSGVPGIKGPRGDPGCPGAEGPAGIPGFPGLKGPKGREGHAGFPGVPGPPGHSCERGAPGIPGQPGLPGYPGSPGAPGGKGQLGDVGPPGPAGMKGLPGLPGWPGAHGPPGLPGIPGPFGDDGLPGPPGPKGPQGLPGFPGFPGERGKPGAEGCPGTKGEPGEKGMSGLPGDRGLRGAKGAIGPPGDEGEMAIISQKGTPGEPGPPGDDGFPGERGDKGTPGMQGRRGEPGRYGPPGFQRGEPGEKGQPGHPGPPGPPGSTGLRGFIGFPGLPGDQGEPGSPGPPGFSGIDGARGPKGNKGDPASHFGPPGPKGEPGSPGCPGHFGASGEQGLPGIQGPRGSPGRPGPPGSSGPPGCPGDHGMPGLRGQPGEMGDPGPRGLQGDPGIPGPPGIKGPSGSPGLNGLHGLKGQKGTKGASGLHDVGPPGPVGIPGLKGERGDPGSPGISPPGPRGEKGPPGPPGSSGPPGPAGATGRAPKDIPDPGPPGDQGPPGPDGPRGAPGPPGLPGSVDLLRGEPGDCGLPGPPGPPGPPGPPGYKGFPGCDGKDGQKGPMGFPGPQGPHGFPGPPGEKGLPGPPGRKGPTGLPGPRGEPGPPADVDDCPRIPGLPGAPGMRGPEGAMGLSGMRGPPGPGCKGEPGLDGRRGVDGVPGSPGPPGRKGDTGEDGYPGGPGPPGPIGDPGPKGFGPGYLGGFLLVLHSQTDQEPTCPLGMPRLWTGYSLLYLEGQEKAHNQDLGLAGSCLPVFSTLPFAYCNIHQVCHYAQRNDRSYWLASAAPLPMMPLSEEAIRPYVSRCAVCEAPAQAVAVHSQDQSIPPCPQTWRSLWIGYSFLMLTL

Target ORF information:

RefSeq Version	XM_009444471.3
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes collagen type IV alpha 4 chain (COL4A4), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009444471.3

ORF Insert Sequence:

ATGTGGTCTC TGCACATAGT ACTAATGAGG TGCTCCTTCA GATTGACCAA GTCCTTGGCC 
ACAGATCCCT GGTCACTTAC ACTCATTCTC TTTTCTGTAC AATATGTATA TGGGAGTGGA 
AAGAAATACG TTGGTCCTTG TGGAGGAAGA GATTGCTCTG TTTGCCACTG TGTTCCTGAA 
AAGGGGTCTC GGGGTCCACC AGGACCACCA GGGCCACAGG GTCCAATTGG ACCCCTGGGA 
GCCCCAGGAC CCATTGGGCT TTCAGGAGAG AAAGGAATGA GAGGGGACCG CGGCCCTCCT 
GGAGCAGCAG GGGACAAAGG AGATAAGGGT TCAACTGGTG TTCCTGGATT TCCAGGTTTA 
GATGGCATAC CTGGGCACCC AGGGCCTCCT GGACCCAGAG GCAAACCTGG TATGAGTGGC 
CACAATGGCT CAAGAGGTGA CCCAGGGTTT CCAGGAGGAA GAGGAGCTCT TGGCCCAGGA 
GGCCCCCTAG GCCATCCTGG GGAAAAGGGA GAAAAAGGAA ATTCAGTGTT CATTTTAGGT 
GCCGTTAAAG GTATTCAGGG AGACAGAGGG GACCCAGGAC TGCCTGGCTT ACCAGGATCT 
TGGGGGGCAG GAGGACCGGC GGGTCCCACA GGATATCCTG GAGAGCCAGG GTTAGTGGGA 
CCTCCGGGCC AACCAGGGCG TCCAGGTTTG AAGGGAAATC CCGGTGTGGG AGTAAAGGGG 
CAAATGGGAG ACCCGGGTGA GGTTGGTCAG CAAGGTTCTC CTGGACCCAC CCTGTTGGTG 
GAGCCACCTG ACTTTCGTCT CTATAAAGGA GAAAAGGGTA TAAAAGGAAT TCCTGGAATG 
GTTGGACTGC CAGGACCACC AGGACGCAAG GGAGAATCTG ATATTGGGGC AAAAGGAGAA 
AAAGGTATTC CTGGATTTCC AGGGCCTCGG GGGGATCCTG GTTCCTATGG ATCTCCAGGT 
TTTCCAGGAT TAAAGGGAGA ACTAGGACTG GTTGGAGATC CTGGGCTATT TGGATTAATT 
GGCCCAAAGG GGGATCCTGG AAATCGAGGG CACCCAGGAC CACCAGGTGT TTTGGTGACT 
CCACCTCTTC CACTCAAAGG CCCACCAGGG GACCCAGGGT TCCCTGGCCG CTATGGAGAA 
ACAGGGGATG TTGGACCACC TGGTCCCCCA GGTCTCTTGG GCAGACCAGG GGAAGCCTGT 
GCAGGCATGA TAGGACCCCC TGGGCCACAA GGATTTCCTG GTCTTCCTGG GCTTCCAGGA 
GAAGCTGGTA TTCCTGGGAG ACCTGATTCT GCTCCAGGAA AACCAGGGAA GCCAGGATCA 
CCTGGCTTGC CTGGAGCACC AGGCCTGCAG GGCCTCCCAG GATCAAGTGT GATATACTGT 
AGTGTTGGGA ATCCTGGACC ACAAGGAATA AAAGGCAAAG TGGGTCCCCC AGGAGGAAGA 
GGCTCAAAAG GAGAAAAAGG AAATGAAGGA CTCTGTGCCT GTGAGCCTGG ACCCATGGGC 
CCCCCTGGCC CTCCAGGACT TCCTGGGAGG CAGGGGAGTA AGGGAGACTT GGGGCTCCCT 
GGCTGGCTTG GAACAAAAGG TGACCCAGGA CCTCCTGGTG CTGAAGGACC TCCAGGGCTA 
CCAGGAAAGC ATGGTGCCTC TGGACCACCT GGCAACAAAG GGGCGAAGGG TGACATGGTT 
GTATCAAGAG TTAAAGGGCA CAAAGGAGAA AGAGGTCCTG ATGGGCCCCC AGGATTTCCA 
GGGCAGCCAG GATCACATGG TCGGGATGGA CATGCTGGAG AAAAAGGGGA TCCAGGACCC 
CCAGGGGATC ATGAAGATGC GACCCCAGGT GGTAAAGGAT TTCCTGGACC TCTGGGCCCC 
CCGGGCAAAG CAGGACCTTT GGGGCCCCCA GGACTGGGAT TTCCTGGTCC ACCAGGACAG 
CGAGGCCACC CAGGAGTTCC AGGCCACCCA GGTGTGAGGG GCCCTGATGG CTTGAAGGGT 
CAGAAAGGTG ACACAATTTC TTGCAACGTA ACCTACCCTG GGAGGCACGG CCCTCCAGGT 
TTTGATGGAC CTCCAGGTCC GAAGGGATTT CCAGGTCCCC AAGGTGCCCC TGGGCTGAGT 
GGTTCAGATG GGCATAAAGG CAGACCTGGC ACACCAGGAA CAGCGGAAAT ACCAGGTCCA 
CCTGGTTTTC GTGGTGACAT GGGAGATCCG GGTTTTGGAG GTGAAAAGGG GTCCTCCCCT 
GCTGGGCCCC CAGGCCCTCC CGGCTCACCA GGAGTGAATG GTCAGAAAGG AATCCCGGGA 
GACCCTGCAT TTGGTCACCT GGGACCCCCG GGAAAGAGGG GTCTTTCAGG AGTGCCAGGG 
ATAAAAGGAC CCAGAGGTGA TCCGGGATGT CCAGGGGCTG AAGGGCCAGC TGGCATTCCT 
GGATTCCCAG GTCTCAAAGG TCCCAAAGGC AGAGAGGGAC ATGCTGGGTT TCCAGGTGTC 
CCAGGTCCAC CTGGCCATTC CTGTGAAAGA GGTGCTCCAG GGATACCAGG GCAACCGGGA 
CTCCCTGGGT ATCCAGGTAG CCCAGGTGCT CCAGGTGGGA AAGGACAGCT GGGAGATGTG 
GGGCCTCCCG GGCCAGCTGG AATGAAAGGC CTCCCTGGAC TCCCAGGATG GCCTGGGGCA 
CATGGTCCCC CAGGCCTCCC AGGAATCCCA GGTCCCTTTG GAGATGATGG GCTACCTGGT 
CCTCCAGGTC CAAAGGGACC CCAGGGGCTG CCTGGTTTCC CAGGTTTTCC CGGAGAAAGA 
GGAAAGCCTG GTGCAGAGGG ATGTCCTGGC ACAAAGGGAG AACCTGGAGA GAAGGGCATG 
TCTGGCCTTC CCGGAGACCG GGGACTGAGA GGGGCCAAAG GAGCCATAGG ACCTCCCGGA 
GATGAAGGAG AAATGGCTAT CATTTCCCAA AAGGGAACAC CTGGGGAACC TGGACCTCCT 
GGAGATGATG GATTCCCAGG AGAAAGAGGT GATAAAGGAA CTCCCGGGAT GCAAGGGAGA 
AGAGGAGAGC CGGGAAGATA CGGACCACCT GGATTTCAGA GAGGGGAACC TGGTGAGAAA 
GGTCAGCCAG GGCATCCTGG ACCCCCAGGC CCTCCAGGCT CAACTGGTCT AAGAGGGTTC 
ATTGGTTTTC CAGGACTTCC AGGTGACCAG GGTGAGCCAG GTTCTCCAGG TCCCCCTGGA 
TTTTCAGGAA TTGATGGAGC AAGAGGACCT AAAGGAAACA AAGGTGACCC TGCCAGTCAC 
TTTGGTCCAC CTGGTCCAAA GGGTGAGCCA GGTAGCCCTG GATGTCCAGG GCATTTTGGA 
GCATCTGGAG AGCAGGGCTT GCCTGGTATT CAAGGGCCCA GAGGATCACC TGGAAGGCCA 
GGGCCACCTG GCTCCTCTGG ACCACCAGGG TGCCCAGGTG ATCACGGGAT GCCTGGGCTG 
AGGGGACAGC CAGGAGAAAT GGGAGACCCT GGGCCAAGAG GCCTCCAGGG GGATCCAGGG 
ATACCAGGTC CTCCGGGAAT AAAAGGTCCC TCCGGATCAC CTGGCCTGAA CGGCTTGCAT 
GGATTGAAAG GTCAGAAAGG AACTAAAGGT GCTTCAGGTT TGCATGATGT GGGGCCACCT 
GGTCCAGTGG GAATACCTGG GCTAAAAGGG GAGAGAGGAG ACCCTGGGAG CCCAGGAATC 
TCTCCTCCAG GTCCTCGTGG AGAAAAAGGT CCCCCAGGAC CCCCAGGGAG TTCAGGACCA 
CCTGGTCCTG CAGGTGCCAC AGGAAGAGCT CCTAAGGACA TTCCTGACCC GGGTCCACCT 
GGAGATCAGG GACCTCCTGG TCCTGATGGC CCAAGAGGAG CACCTGGGCC TCCAGGCCTC 
CCTGGGAGTG TTGACCTTCT GAGAGGGGAG CCAGGTGACT GTGGTCTACC AGGGCCACCA 
GGTCCCCCTG GCCCACCAGG CCCTCCAGGA TACAAAGGCT TTCCAGGATG TGATGGAAAA 
GATGGCCAGA AAGGACCAAT GGGATTCCCG GGACCGCAGG GACCACATGG ATTTCCTGGG 
CCACCTGGAG AGAAGGGTTT ACCTGGACCT CCAGGGAGAA AAGGGCCCAC TGGTCTTCCA 
GGTCCCAGAG GTGAACCGGG GCCACCTGCA GATGTGGATG ACTGTCCCCG AATCCCAGGC 
CTTCCTGGGG CGCCAGGCAT GAGAGGACCA GAAGGAGCCA TGGGGCTCTC TGGAATGAGA 
GGCCCCCCAG GACCAGGGTG CAAAGGAGAG CCTGGGCTGG ATGGCAGGAG GGGTGTGGAT 
GGCGTCCCTG GGTCTCCTGG GCCTCCCGGA CGTAAAGGTG ACACAGGAGA AGACGGCTAC 
CCTGGAGGAC CAGGGCCTCC TGGTCCCATT GGGGATCCTG GGCCCAAAGG GTTTGGCCCT 
GGATACCTCG GTGGCTTCCT CCTGGTTCTC CACAGTCAGA CGGACCAGGA GCCCACCTGC 
CCCCTGGGCA TGCCCAGGCT CTGGACTGGG TATAGTCTGT TATACCTGGA AGGGCAAGAG 
AAAGCTCACA ATCAAGACCT TGGTCTGGCA GGGTCTTGCC TTCCCGTGTT TAGCACGCTG 
CCCTTTGCCT ACTGCAACAT CCACCAGGTG TGCCACTATG CCCAGAGAAA CGACAGATCC 
TACTGGCTGG CCAGCGCTGC GCCCCTCCCC ATGATGCCAC TCTCTGAAGA GGCGATCCGC 
CCCTATGTCA GCCGCTGTGC GGTATGCGAG GCCCCGGCCC AGGCGGTGGC GGTGCACAGC 
CAGGACCAGT CCATCCCCCC ATGTCCGCAG ACCTGGAGGA GCCTCTGGAT CGGGTATTCA 
TTCCTGATGC TTACATTGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL4A4 ( XM_009444465.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_009442740.2 Pan troglodytes collagen type IV alpha 4 (COL4A4), transcript variant X4, mRNA.

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CloneID	OPc80763
Clone ID Related Accession (Same CDS sequence)	XM_009444465.2 , XM_516128.4 , XM_016950591.1 , XM_009444464.2
Accession Version	XM_009444465.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5073bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	collagen alpha-4(IV) chain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006470.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 2, 2016 this sequence version replaced XM_009444465.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGTCTC TGCACATAGT ACTAATGAGG TGCTCCTTCA GATTGACCAA GTCCTTGGCC 
ACAGATCCCT GGTCACTTAC ACTCATTCTC TTTTCTGTAC AATATGTATA TGGGAGTGGA 
AAGAAATACG TTGGTCCTTG TGGAGGAAGA GATTGCTCTG TTTGCCACTG TGTTCCTGAA 
AAGGGGTCTC GGGGTCCACC AGGACCACCA GGGCCACAGG GTCCAATTGG ACCCCTGGGA 
GCCCCAGGAC CCATTGGGCT TTCAGGAGAG AAAGGAATGA GAGGGGACCA CGGCCCTCCT 
GGAGCAGCAG GGGACAAAGG AGATAAGGGT TCAACTGGTG TTCCTGGATT TCCAGGTTTA 
GATGGCATAC CTGGGCACCC AGGGCCTCCT GGACCCAGAG GCAAACCTGG TATGAGTGGC 
CACAATGGCT CAAGAGGTGA CCCAGGGTTT CCAGGAGGAA GAGGAGCTCT TGGCCCAGGA 
GGCCCCCTAG GCCATCCTGG GGAAAAGGGA GAAAAAGGAA ATTCAGTGTT CATTTTAGGT 
GCCGTTAAAG GTATTCAGGG AGACAGAGGG GACCCAGGAC TGCCTGGCTT ACCAGGATCT 
TGGGGGGCAG GAGGACCGGC GGGTCCCACA GGATATCCTG GAGAGCCAGG GTTAGTGGGA 
CCTCCGGGCC AACCAGGGCG TCCAGGTTTG AAGGGAAATC CCGGTGTGGG AGTAAAGGGG 
CAAATGGGAG ACCCGGGTGA GGTTGGTCAG CAAGGTTCTC CTGGACCCAC CCTGTTGGTG 
GAGCCACCTG ACTTTCGTCT CTATAAAGGA GAAAAGGGTA TAAAAGGAAT TCCTGGAATG 
GTTGGACTGC CAGGACCACC AGGACGCAAG GGAGAATCTG ATATTGGGGC AAAAGGAGAA 
AAAGGTATTC CTGGATTTCC AGGGCCTCGG GGGGATCCTG GTTCCTATGG ATCTCCAGGT 
TTTCCAGGAT TAAAGGGAGA ACTAGGACTG GTTGGAGATC CTGGGCTATT TGGATTAATT 
GGCCCAAAGG GGGATCCTGG AAATCGAGGG CACCCAGGAC CACCAGGTGT TTTGGTGACT 
CCACCTCTTC CACTCAAAGG CCCACCAGGG GACCCAGGGT TCCCTGGCCG CTATGGAGAA 
ACAGGGGATG TTGGACCACC TGGTCCCCCA GGTCTCTTGG GCAGACCAGG GGAAGCCTGT 
GCAGGCATGA TAGGACCCCC TGGGCCACAA GGATTTCCTG GTCTTCCTGG GCTTCCAGGA 
GAAGCTGGTA TTCCTGGGAG ACCTGATTCT GCTCCAGGAA AACCAGGGAA GCCAGGATCA 
CCTGGCTTGC CTGGAGCACC AGGCCTGCAG GGCCTCCCAG GATCAAGTGT GATATACTGT 
AGTGTTGGGA ATCCTGGACC ACAAGGAATA AAAGGCAAAG TGGGTCCCCC AGGAGGAAGA 
GGCTCAAAAG GAGAAAAAGG AAATGAAGGA CTCTGTGCCT GTGAGCCTGG ACCCATGGGC 
CCCCCTGGCC CTCCAGGACT TCCTGGGAGG CAGGGGAGTA AGGGAGACTT GGGGCTCCCT 
GGCTGGCTTG GAACAAAAGG TGACCCAGGA CCTCCTGGTG CTGAAGGACC TCCAGGGCTA 
CCAGGAAAGC ATGGTGCCTC TGGACCACCT GGCAACAAAG GGGCGAAGGG TGACATGGTT 
GTATCAAGAG TTAAAGGGCA CAAAGGAGAA AGAGGTCCTG ATGGGCCCCC AGGATTTCCA 
GGGCAGCCAG GATCACATGG TCGGGATGGA CATGCTGGAG AAAAAGGGGA TCCAGGACCC 
CCAGGGGATC ATGAAGATGC GACCCCAGGT GGTAAAGGAT TTCCTGGACC TCTGGGCCCC 
CCGGGCAAAG CAGGACCTTT GGGGCCCCCA GGACTGGGAT TTCCTGGTCC ACCAGGACAG 
CGAGGCCACC CAGGAGTTCC AGGCCACCCA GGTGTGAGGG GCCCTGATGG CTTGAAGGGT 
CAGAAAGGTG ACACAATTTC TTGCAACGTA ACCTACCCTG GGAGGCACGG CCCTCCAGGT 
TTTGATGGAC CTCCAGGTCC GAAGGGATTT CCAGGTCCCC AAGGTGCCCC TGGGCTGAGT 
GGTTCAGATG GGCATAAAGG CAGACCTGGC ACACCAGGAA CAGCGGAAAT ACCAGGTCCA 
CCTGGTTTTC GTGGTGACAT GGGAGATCCG GGTTTTGGAG GTGAAAAGGG GTCCTCCCCT 
GCTGGGCCCC CAGGCCCTCC CGGCTCACCA GGAGTGAATG GTCAGAAAGG AATCCCGGGA 
GACCCTGCAT TTGGTCACCT GGGACCCCCG GGAAAGAGGG GTCTTTCAGG AGTGCCAGGG 
ATAAAAGGAC CCAGAGGTGA TCCGGGATGT CCAGGGGCTG AAGGGCCAGC TGGCATTCCT 
GGATTCCCAG GTCTCAAAGG TCCCAAAGGC AGAGAGGGAC ATGCTGGGTT TCCAGGTGTC 
CCAGGTCCAC CTGGCCATTC CTGTGAAAGA GGTGCTCCAG GGATACCAGG GCAACCGGGA 
CTCCCTGGGT ATCCAGGTAG CCCAGGTGCT CCAGGTGGGA AAGGACAGCT GGGAGATGTG 
GGGCCTCCCG GGCCAGCTGG AATGAAAGGC CTCCCTGGAC TCCCAGGATG GCCTGGGGCA 
CATGGTCCCC CAGGCCTCCC AGGAATCCCA GGTCCCTTTG GAGATGATGG GCTACCTGGT 
CCTCCAGGTC CAAAGGGACC CCAGGGGCTG CCTGGTTTCC CAGGTTTTCC CGGAGAAAGA 
GGAAAGCCTG GTGCAGAGGG ATGTCCTGGC ACAAAGGGAG AACCTGGAGA GAAGGGCATG 
TCTGGCCTTC CCGGAGACCG GGGACTGAGA GGGGCCAAAG GAGCCATAGG ACCTCCCGGA 
GATGAAGGAG AAATGGCTAT CATTTCCCAA AAGGGAACAC CTGGGGAACC TGGACCTCCT 
GGAGATGATG GATTCCCAGG AGAAAGAGGT GATAAAGGAA CTCCCGGGAT GCAAGGGAGA 
AGAGGAGAGC CGGGAAGATA CGGACCACCT GGATTTCAGA GAGGGGAACC TGGTGAGAAA 
GGTCAGCCAG GGCATCCTGG ACCCCCAGGC CCTCCAGGCT CAACTGGTCT AAGAGGGTTC 
ATTGGTTTTC CAGGACTTCC AGGTGACCAG GGTGAGCCAG GTTCTCCAGG TCCCCCTGGA 
TTTTCAGGAA TTGATGGAGC AAGAGGACCT AAAGGAAACA AAGGTGACCC TGCCAGTCAC 
TTTGGTCCAC CTGGTCCAAA GGGTGAGCCA GGTAGCCCTG GATGTCCAGG GCATTTTGGA 
GCATCTGGAG AGCAGGGCTT GCCTGGTATT CAAGGGCCCA GAGGATCACC TGGAAGGCCA 
GGGCCACCTG GCTCCTCTGG ACCACCAGGG TGCCCAGGTG ATCACGGGAT GCCTGGGCTG 
AGGGGACAGC CAGGAGAAAT GGGAGACCCT GGGCCAAGAG GCCTCCAGGG GGATCCAGGG 
ATACCAGGTC CTCCGGGAAT AAAAGGTCCC TCCGGATCAC CTGGCCTGAA CGGCTTGCAT 
GGATTGAAAG GTCAGAAAGG AACTAAAGGT GCTTCAGGTT TGCATGATGT GGGGCCACCT 
GGTCCAGTGG GAATACCTGG GCTAAAAGGG GAGAGAGGAG ACCCTGGGAG CCCAGGAATC 
TCTCCTCCAG GTCCTCGTGG AGAAAAAGGT CCCCCAGGAC CCCCAGGGAG TTCAGGACCA 
CCTGGTCCTG CAGGTGCCAC AGGAAGAGCT CCTAAGGACA TTCCTGACCC GGGTCCACCT 
GGAGATCAGG GACCTCCTGG TCCTGATGGC CCAAGAGGAG CACCTGGGCC TCCAGGCCTC 
CCTGGGAGTG TTGACCTTCT GAGAGGGGAG CCAGGTGACT GTGGTCTACC AGGGCCACCA 
GGTCCCCCTG GCCCACCAGG CCCTCCAGGA TACAAAGGCT TTCCAGGATG TGATGGAAAA 
GATGGCCAGA AAGGACCAAT GGGATTCCCG GGACCGCAGG GACCACATGG ATTTCCTGGG 
CCACCTGGAG AGAAGGGTTT ACCTGGACCT CCAGGGAGAA AAGGGCCCAC TGGTCTTCCA 
GGTCCCAGAG GTGAACCGGG GCCACCTGCA GATGTGGATG ACTGTCCCCG AATCCCAGGC 
CTTCCTGGGG CGCCAGGCAT GAGAGGACCA GAAGGAGCCA TGGGGCTCTC TGGAATGAGA 
GGCCCCCCAG GACCAGGGTG CAAAGGAGAG CCTGGGCTGG ATGGCAGGAG GGGTGTGGAT 
GGCGTCCCTG GGTCTCCTGG GCCTCCCGGA CGTAAAGGTG ACACAGGAGA AGACGGCTAC 
CCTGGAGGAC CAGGGCCTCC TGGTCCCATT GGGGATCCTG GGCCCAAAGG GTTTGGCCCT 
GGATACCTCG GTGGCTTCCT CCTGGTTCTC CACAGTCAGA CGGACCAGGA GCCCACCTGC 
CCCCTGGGCA TGCCCAGGCT CTGGACTGGG TATAGTCTGT TATACCTGGA AGGGCAAGAG 
AAAGCTCACA ATCAAGACCT TGGTCTGGCA GGGTCTTGCC TTCCCGTGTT TAGCACGCTG 
CCCTTTGCCT ACTGCAACAT CCACCAGGTG TGCCACTATG CCCAGAGAAA CGACAGATCC 
TACTGGCTGG CCAGCGCTGC GCCCCTCCCC ATGATGCCAC TCTCTGAAGA GGCGATCCGC 
CCCTATGTCA GCCGCTGTGC GGTATGCGAG GCCCCGGCCC AGGCGGTGGC GGTGCACAGC 
CAGGACAAGT CCATCCCCCC ATGTCCGCAG ACCTGGAGGA GCCTCTGGAT CGGGTATTCA 
TTCCTGATGC ACACAGGAGC TGGGGACCAA GGAGGAGGGC AGGCCCTTAT GTCACCTGGC 
AGCTGCCTGG AAGATTTCAG AGCAGCACCA TTCCTTGAAT GCCAGGGCCG GCAGGGAACT 
TGCCACTTTT TTGCAAATAA GTATAGCTTC TGGCTCACAA CGGTGAAAGC AGACTTGCAG 
TTTTCCTCTG CTCCAGCACC AGACACCTTA AAAGAAAGCC AGGCCCAACG CCAGAAAATC 
AGCCGGTGCC AGGTCTGCGT GAAGTATAGC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009442740.2
CDS	533..5605
Translation	MWSLHIVLMRCSFRLTKSLATDPWSLTLILFSVQYVYGSGKKYVGPCGGRDCSVCHCVPEKGSRGPPGPPGPQGPIGPLGAPGPIGLSGEKGMRGDHGPPGAAGDKGDKGSTGVPGFPGLDGIPGHPGPPGPRGKPGMSGHNGSRGDPGFPGGRGALGPGGPLGHPGEKGEKGNSVFILGAVKGIQGDRGDPGLPGLPGSWGAGGPAGPTGYPGEPGLVGPPGQPGRPGLKGNPGVGVKGQMGDPGEVGQQGSPGPTLLVEPPDFRLYKGEKGIKGIPGMVGLPGPPGRKGESDIGAKGEKGIPGFPGPRGDPGSYGSPGFPGLKGELGLVGDPGLFGLIGPKGDPGNRGHPGPPGVLVTPPLPLKGPPGDPGFPGRYGETGDVGPPGPPGLLGRPGEACAGMIGPPGPQGFPGLPGLPGEAGIPGRPDSAPGKPGKPGSPGLPGAPGLQGLPGSSVIYCSVGNPGPQGIKGKVGPPGGRGSKGEKGNEGLCACEPGPMGPPGPPGLPGRQGSKGDLGLPGWLGTKGDPGPPGAEGPPGLPGKHGASGPPGNKGAKGDMVVSRVKGHKGERGPDGPPGFPGQPGSHGRDGHAGEKGDPGPPGDHEDATPGGKGFPGPLGPPGKAGPLGPPGLGFPGPPGQRGHPGVPGHPGVRGPDGLKGQKGDTISCNVTYPGRHGPPGFDGPPGPKGFPGPQGAPGLSGSDGHKGRPGTPGTAEIPGPPGFRGDMGDPGFGGEKGSSPAGPPGPPGSPGVNGQKGIPGDPAFGHLGPPGKRGLSGVPGIKGPRGDPGCPGAEGPAGIPGFPGLKGPKGREGHAGFPGVPGPPGHSCERGAPGIPGQPGLPGYPGSPGAPGGKGQLGDVGPPGPAGMKGLPGLPGWPGAHGPPGLPGIPGPFGDDGLPGPPGPKGPQGLPGFPGFPGERGKPGAEGCPGTKGEPGEKGMSGLPGDRGLRGAKGAIGPPGDEGEMAIISQKGTPGEPGPPGDDGFPGERGDKGTPGMQGRRGEPGRYGPPGFQRGEPGEKGQPGHPGPPGPPGSTGLRGFIGFPGLPGDQGEPGSPGPPGFSGIDGARGPKGNKGDPASHFGPPGPKGEPGSPGCPGHFGASGEQGLPGIQGPRGSPGRPGPPGSSGPPGCPGDHGMPGLRGQPGEMGDPGPRGLQGDPGIPGPPGIKGPSGSPGLNGLHGLKGQKGTKGASGLHDVGPPGPVGIPGLKGERGDPGSPGISPPGPRGEKGPPGPPGSSGPPGPAGATGRAPKDIPDPGPPGDQGPPGPDGPRGAPGPPGLPGSVDLLRGEPGDCGLPGPPGPPGPPGPPGYKGFPGCDGKDGQKGPMGFPGPQGPHGFPGPPGEKGLPGPPGRKGPTGLPGPRGEPGPPADVDDCPRIPGLPGAPGMRGPEGAMGLSGMRGPPGPGCKGEPGLDGRRGVDGVPGSPGPPGRKGDTGEDGYPGGPGPPGPIGDPGPKGFGPGYLGGFLLVLHSQTDQEPTCPLGMPRLWTGYSLLYLEGQEKAHNQDLGLAGSCLPVFSTLPFAYCNIHQVCHYAQRNDRSYWLASAAPLPMMPLSEEAIRPYVSRCAVCEAPAQAVAVHSQDKSIPPCPQTWRSLWIGYSFLMHTGAGDQGGGQALMSPGSCLEDFRAAPFLECQGRQGTCHFFANKYSFWLTTVKADLQFSSAPAPDTLKESQAQRQKISRCQVCVKYS

Target ORF information:

RefSeq Version	XM_009444465.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes collagen type IV alpha 4 (COL4A4), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009444465.2

ORF Insert Sequence:

ATGTGGTCTC TGCACATAGT ACTAATGAGG TGCTCCTTCA GATTGACCAA GTCCTTGGCC 
ACAGATCCCT GGTCACTTAC ACTCATTCTC TTTTCTGTAC AATATGTATA TGGGAGTGGA 
AAGAAATACG TTGGTCCTTG TGGAGGAAGA GATTGCTCTG TTTGCCACTG TGTTCCTGAA 
AAGGGGTCTC GGGGTCCACC AGGACCACCA GGGCCACAGG GTCCAATTGG ACCCCTGGGA 
GCCCCAGGAC CCATTGGGCT TTCAGGAGAG AAAGGAATGA GAGGGGACCA CGGCCCTCCT 
GGAGCAGCAG GGGACAAAGG AGATAAGGGT TCAACTGGTG TTCCTGGATT TCCAGGTTTA 
GATGGCATAC CTGGGCACCC AGGGCCTCCT GGACCCAGAG GCAAACCTGG TATGAGTGGC 
CACAATGGCT CAAGAGGTGA CCCAGGGTTT CCAGGAGGAA GAGGAGCTCT TGGCCCAGGA 
GGCCCCCTAG GCCATCCTGG GGAAAAGGGA GAAAAAGGAA ATTCAGTGTT CATTTTAGGT 
GCCGTTAAAG GTATTCAGGG AGACAGAGGG GACCCAGGAC TGCCTGGCTT ACCAGGATCT 
TGGGGGGCAG GAGGACCGGC GGGTCCCACA GGATATCCTG GAGAGCCAGG GTTAGTGGGA 
CCTCCGGGCC AACCAGGGCG TCCAGGTTTG AAGGGAAATC CCGGTGTGGG AGTAAAGGGG 
CAAATGGGAG ACCCGGGTGA GGTTGGTCAG CAAGGTTCTC CTGGACCCAC CCTGTTGGTG 
GAGCCACCTG ACTTTCGTCT CTATAAAGGA GAAAAGGGTA TAAAAGGAAT TCCTGGAATG 
GTTGGACTGC CAGGACCACC AGGACGCAAG GGAGAATCTG ATATTGGGGC AAAAGGAGAA 
AAAGGTATTC CTGGATTTCC AGGGCCTCGG GGGGATCCTG GTTCCTATGG ATCTCCAGGT 
TTTCCAGGAT TAAAGGGAGA ACTAGGACTG GTTGGAGATC CTGGGCTATT TGGATTAATT 
GGCCCAAAGG GGGATCCTGG AAATCGAGGG CACCCAGGAC CACCAGGTGT TTTGGTGACT 
CCACCTCTTC CACTCAAAGG CCCACCAGGG GACCCAGGGT TCCCTGGCCG CTATGGAGAA 
ACAGGGGATG TTGGACCACC TGGTCCCCCA GGTCTCTTGG GCAGACCAGG GGAAGCCTGT 
GCAGGCATGA TAGGACCCCC TGGGCCACAA GGATTTCCTG GTCTTCCTGG GCTTCCAGGA 
GAAGCTGGTA TTCCTGGGAG ACCTGATTCT GCTCCAGGAA AACCAGGGAA GCCAGGATCA 
CCTGGCTTGC CTGGAGCACC AGGCCTGCAG GGCCTCCCAG GATCAAGTGT GATATACTGT 
AGTGTTGGGA ATCCTGGACC ACAAGGAATA AAAGGCAAAG TGGGTCCCCC AGGAGGAAGA 
GGCTCAAAAG GAGAAAAAGG AAATGAAGGA CTCTGTGCCT GTGAGCCTGG ACCCATGGGC 
CCCCCTGGCC CTCCAGGACT TCCTGGGAGG CAGGGGAGTA AGGGAGACTT GGGGCTCCCT 
GGCTGGCTTG GAACAAAAGG TGACCCAGGA CCTCCTGGTG CTGAAGGACC TCCAGGGCTA 
CCAGGAAAGC ATGGTGCCTC TGGACCACCT GGCAACAAAG GGGCGAAGGG TGACATGGTT 
GTATCAAGAG TTAAAGGGCA CAAAGGAGAA AGAGGTCCTG ATGGGCCCCC AGGATTTCCA 
GGGCAGCCAG GATCACATGG TCGGGATGGA CATGCTGGAG AAAAAGGGGA TCCAGGACCC 
CCAGGGGATC ATGAAGATGC GACCCCAGGT GGTAAAGGAT TTCCTGGACC TCTGGGCCCC 
CCGGGCAAAG CAGGACCTTT GGGGCCCCCA GGACTGGGAT TTCCTGGTCC ACCAGGACAG 
CGAGGCCACC CAGGAGTTCC AGGCCACCCA GGTGTGAGGG GCCCTGATGG CTTGAAGGGT 
CAGAAAGGTG ACACAATTTC TTGCAACGTA ACCTACCCTG GGAGGCACGG CCCTCCAGGT 
TTTGATGGAC CTCCAGGTCC GAAGGGATTT CCAGGTCCCC AAGGTGCCCC TGGGCTGAGT 
GGTTCAGATG GGCATAAAGG CAGACCTGGC ACACCAGGAA CAGCGGAAAT ACCAGGTCCA 
CCTGGTTTTC GTGGTGACAT GGGAGATCCG GGTTTTGGAG GTGAAAAGGG GTCCTCCCCT 
GCTGGGCCCC CAGGCCCTCC CGGCTCACCA GGAGTGAATG GTCAGAAAGG AATCCCGGGA 
GACCCTGCAT TTGGTCACCT GGGACCCCCG GGAAAGAGGG GTCTTTCAGG AGTGCCAGGG 
ATAAAAGGAC CCAGAGGTGA TCCGGGATGT CCAGGGGCTG AAGGGCCAGC TGGCATTCCT 
GGATTCCCAG GTCTCAAAGG TCCCAAAGGC AGAGAGGGAC ATGCTGGGTT TCCAGGTGTC 
CCAGGTCCAC CTGGCCATTC CTGTGAAAGA GGTGCTCCAG GGATACCAGG GCAACCGGGA 
CTCCCTGGGT ATCCAGGTAG CCCAGGTGCT CCAGGTGGGA AAGGACAGCT GGGAGATGTG 
GGGCCTCCCG GGCCAGCTGG AATGAAAGGC CTCCCTGGAC TCCCAGGATG GCCTGGGGCA 
CATGGTCCCC CAGGCCTCCC AGGAATCCCA GGTCCCTTTG GAGATGATGG GCTACCTGGT 
CCTCCAGGTC CAAAGGGACC CCAGGGGCTG CCTGGTTTCC CAGGTTTTCC CGGAGAAAGA 
GGAAAGCCTG GTGCAGAGGG ATGTCCTGGC ACAAAGGGAG AACCTGGAGA GAAGGGCATG 
TCTGGCCTTC CCGGAGACCG GGGACTGAGA GGGGCCAAAG GAGCCATAGG ACCTCCCGGA 
GATGAAGGAG AAATGGCTAT CATTTCCCAA AAGGGAACAC CTGGGGAACC TGGACCTCCT 
GGAGATGATG GATTCCCAGG AGAAAGAGGT GATAAAGGAA CTCCCGGGAT GCAAGGGAGA 
AGAGGAGAGC CGGGAAGATA CGGACCACCT GGATTTCAGA GAGGGGAACC TGGTGAGAAA 
GGTCAGCCAG GGCATCCTGG ACCCCCAGGC CCTCCAGGCT CAACTGGTCT AAGAGGGTTC 
ATTGGTTTTC CAGGACTTCC AGGTGACCAG GGTGAGCCAG GTTCTCCAGG TCCCCCTGGA 
TTTTCAGGAA TTGATGGAGC AAGAGGACCT AAAGGAAACA AAGGTGACCC TGCCAGTCAC 
TTTGGTCCAC CTGGTCCAAA GGGTGAGCCA GGTAGCCCTG GATGTCCAGG GCATTTTGGA 
GCATCTGGAG AGCAGGGCTT GCCTGGTATT CAAGGGCCCA GAGGATCACC TGGAAGGCCA 
GGGCCACCTG GCTCCTCTGG ACCACCAGGG TGCCCAGGTG ATCACGGGAT GCCTGGGCTG 
AGGGGACAGC CAGGAGAAAT GGGAGACCCT GGGCCAAGAG GCCTCCAGGG GGATCCAGGG 
ATACCAGGTC CTCCGGGAAT AAAAGGTCCC TCCGGATCAC CTGGCCTGAA CGGCTTGCAT 
GGATTGAAAG GTCAGAAAGG AACTAAAGGT GCTTCAGGTT TGCATGATGT GGGGCCACCT 
GGTCCAGTGG GAATACCTGG GCTAAAAGGG GAGAGAGGAG ACCCTGGGAG CCCAGGAATC 
TCTCCTCCAG GTCCTCGTGG AGAAAAAGGT CCCCCAGGAC CCCCAGGGAG TTCAGGACCA 
CCTGGTCCTG CAGGTGCCAC AGGAAGAGCT CCTAAGGACA TTCCTGACCC GGGTCCACCT 
GGAGATCAGG GACCTCCTGG TCCTGATGGC CCAAGAGGAG CACCTGGGCC TCCAGGCCTC 
CCTGGGAGTG TTGACCTTCT GAGAGGGGAG CCAGGTGACT GTGGTCTACC AGGGCCACCA 
GGTCCCCCTG GCCCACCAGG CCCTCCAGGA TACAAAGGCT TTCCAGGATG TGATGGAAAA 
GATGGCCAGA AAGGACCAAT GGGATTCCCG GGACCGCAGG GACCACATGG ATTTCCTGGG 
CCACCTGGAG AGAAGGGTTT ACCTGGACCT CCAGGGAGAA AAGGGCCCAC TGGTCTTCCA 
GGTCCCAGAG GTGAACCGGG GCCACCTGCA GATGTGGATG ACTGTCCCCG AATCCCAGGC 
CTTCCTGGGG CGCCAGGCAT GAGAGGACCA GAAGGAGCCA TGGGGCTCTC TGGAATGAGA 
GGCCCCCCAG GACCAGGGTG CAAAGGAGAG CCTGGGCTGG ATGGCAGGAG GGGTGTGGAT 
GGCGTCCCTG GGTCTCCTGG GCCTCCCGGA CGTAAAGGTG ACACAGGAGA AGACGGCTAC 
CCTGGAGGAC CAGGGCCTCC TGGTCCCATT GGGGATCCTG GGCCCAAAGG GTTTGGCCCT 
GGATACCTCG GTGGCTTCCT CCTGGTTCTC CACAGTCAGA CGGACCAGGA GCCCACCTGC 
CCCCTGGGCA TGCCCAGGCT CTGGACTGGG TATAGTCTGT TATACCTGGA AGGGCAAGAG 
AAAGCTCACA ATCAAGACCT TGGTCTGGCA GGGTCTTGCC TTCCCGTGTT TAGCACGCTG 
CCCTTTGCCT ACTGCAACAT CCACCAGGTG TGCCACTATG CCCAGAGAAA CGACAGATCC 
TACTGGCTGG CCAGCGCTGC GCCCCTCCCC ATGATGCCAC TCTCTGAAGA GGCGATCCGC 
CCCTATGTCA GCCGCTGTGC GGTATGCGAG GCCCCGGCCC AGGCGGTGGC GGTGCACAGC 
CAGGACAAGT CCATCCCCCC ATGTCCGCAG ACCTGGAGGA GCCTCTGGAT CGGGTATTCA 
TTCCTGATGC ACACAGGAGC TGGGGACCAA GGAGGAGGGC AGGCCCTTAT GTCACCTGGC 
AGCTGCCTGG AAGATTTCAG AGCAGCACCA TTCCTTGAAT GCCAGGGCCG GCAGGGAACT 
TGCCACTTTT TTGCAAATAA GTATAGCTTC TGGCTCACAA CGGTGAAAGC AGACTTGCAG 
TTTTCCTCTG CTCCAGCACC AGACACCTTA AAAGAAAGCC AGGCCCAACG CCAGAAAATC 
AGCCGGTGCC AGGTCTGCGT GAAGTATAGC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL4A4 ( XM_516128.4 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_516128.3 Pan troglodytes collagen type IV alpha 4 (COL4A4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OPc80763
Clone ID Related Accession (Same CDS sequence)	XM_009444465.2 , XM_516128.4 , XM_016950591.1 , XM_009444464.2
Accession Version	XM_516128.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5073bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	collagen alpha-4(IV) chain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006470.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 2, 2016 this sequence version replaced XM_516128.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGTCTC TGCACATAGT ACTAATGAGG TGCTCCTTCA GATTGACCAA GTCCTTGGCC 
ACAGATCCCT GGTCACTTAC ACTCATTCTC TTTTCTGTAC AATATGTATA TGGGAGTGGA 
AAGAAATACG TTGGTCCTTG TGGAGGAAGA GATTGCTCTG TTTGCCACTG TGTTCCTGAA 
AAGGGGTCTC GGGGTCCACC AGGACCACCA GGGCCACAGG GTCCAATTGG ACCCCTGGGA 
GCCCCAGGAC CCATTGGGCT TTCAGGAGAG AAAGGAATGA GAGGGGACCA CGGCCCTCCT 
GGAGCAGCAG GGGACAAAGG AGATAAGGGT TCAACTGGTG TTCCTGGATT TCCAGGTTTA 
GATGGCATAC CTGGGCACCC AGGGCCTCCT GGACCCAGAG GCAAACCTGG TATGAGTGGC 
CACAATGGCT CAAGAGGTGA CCCAGGGTTT CCAGGAGGAA GAGGAGCTCT TGGCCCAGGA 
GGCCCCCTAG GCCATCCTGG GGAAAAGGGA GAAAAAGGAA ATTCAGTGTT CATTTTAGGT 
GCCGTTAAAG GTATTCAGGG AGACAGAGGG GACCCAGGAC TGCCTGGCTT ACCAGGATCT 
TGGGGGGCAG GAGGACCGGC GGGTCCCACA GGATATCCTG GAGAGCCAGG GTTAGTGGGA 
CCTCCGGGCC AACCAGGGCG TCCAGGTTTG AAGGGAAATC CCGGTGTGGG AGTAAAGGGG 
CAAATGGGAG ACCCGGGTGA GGTTGGTCAG CAAGGTTCTC CTGGACCCAC CCTGTTGGTG 
GAGCCACCTG ACTTTCGTCT CTATAAAGGA GAAAAGGGTA TAAAAGGAAT TCCTGGAATG 
GTTGGACTGC CAGGACCACC AGGACGCAAG GGAGAATCTG ATATTGGGGC AAAAGGAGAA 
AAAGGTATTC CTGGATTTCC AGGGCCTCGG GGGGATCCTG GTTCCTATGG ATCTCCAGGT 
TTTCCAGGAT TAAAGGGAGA ACTAGGACTG GTTGGAGATC CTGGGCTATT TGGATTAATT 
GGCCCAAAGG GGGATCCTGG AAATCGAGGG CACCCAGGAC CACCAGGTGT TTTGGTGACT 
CCACCTCTTC CACTCAAAGG CCCACCAGGG GACCCAGGGT TCCCTGGCCG CTATGGAGAA 
ACAGGGGATG TTGGACCACC TGGTCCCCCA GGTCTCTTGG GCAGACCAGG GGAAGCCTGT 
GCAGGCATGA TAGGACCCCC TGGGCCACAA GGATTTCCTG GTCTTCCTGG GCTTCCAGGA 
GAAGCTGGTA TTCCTGGGAG ACCTGATTCT GCTCCAGGAA AACCAGGGAA GCCAGGATCA 
CCTGGCTTGC CTGGAGCACC AGGCCTGCAG GGCCTCCCAG GATCAAGTGT GATATACTGT 
AGTGTTGGGA ATCCTGGACC ACAAGGAATA AAAGGCAAAG TGGGTCCCCC AGGAGGAAGA 
GGCTCAAAAG GAGAAAAAGG AAATGAAGGA CTCTGTGCCT GTGAGCCTGG ACCCATGGGC 
CCCCCTGGCC CTCCAGGACT TCCTGGGAGG CAGGGGAGTA AGGGAGACTT GGGGCTCCCT 
GGCTGGCTTG GAACAAAAGG TGACCCAGGA CCTCCTGGTG CTGAAGGACC TCCAGGGCTA 
CCAGGAAAGC ATGGTGCCTC TGGACCACCT GGCAACAAAG GGGCGAAGGG TGACATGGTT 
GTATCAAGAG TTAAAGGGCA CAAAGGAGAA AGAGGTCCTG ATGGGCCCCC AGGATTTCCA 
GGGCAGCCAG GATCACATGG TCGGGATGGA CATGCTGGAG AAAAAGGGGA TCCAGGACCC 
CCAGGGGATC ATGAAGATGC GACCCCAGGT GGTAAAGGAT TTCCTGGACC TCTGGGCCCC 
CCGGGCAAAG CAGGACCTTT GGGGCCCCCA GGACTGGGAT TTCCTGGTCC ACCAGGACAG 
CGAGGCCACC CAGGAGTTCC AGGCCACCCA GGTGTGAGGG GCCCTGATGG CTTGAAGGGT 
CAGAAAGGTG ACACAATTTC TTGCAACGTA ACCTACCCTG GGAGGCACGG CCCTCCAGGT 
TTTGATGGAC CTCCAGGTCC GAAGGGATTT CCAGGTCCCC AAGGTGCCCC TGGGCTGAGT 
GGTTCAGATG GGCATAAAGG CAGACCTGGC ACACCAGGAA CAGCGGAAAT ACCAGGTCCA 
CCTGGTTTTC GTGGTGACAT GGGAGATCCG GGTTTTGGAG GTGAAAAGGG GTCCTCCCCT 
GCTGGGCCCC CAGGCCCTCC CGGCTCACCA GGAGTGAATG GTCAGAAAGG AATCCCGGGA 
GACCCTGCAT TTGGTCACCT GGGACCCCCG GGAAAGAGGG GTCTTTCAGG AGTGCCAGGG 
ATAAAAGGAC CCAGAGGTGA TCCGGGATGT CCAGGGGCTG AAGGGCCAGC TGGCATTCCT 
GGATTCCCAG GTCTCAAAGG TCCCAAAGGC AGAGAGGGAC ATGCTGGGTT TCCAGGTGTC 
CCAGGTCCAC CTGGCCATTC CTGTGAAAGA GGTGCTCCAG GGATACCAGG GCAACCGGGA 
CTCCCTGGGT ATCCAGGTAG CCCAGGTGCT CCAGGTGGGA AAGGACAGCT GGGAGATGTG 
GGGCCTCCCG GGCCAGCTGG AATGAAAGGC CTCCCTGGAC TCCCAGGATG GCCTGGGGCA 
CATGGTCCCC CAGGCCTCCC AGGAATCCCA GGTCCCTTTG GAGATGATGG GCTACCTGGT 
CCTCCAGGTC CAAAGGGACC CCAGGGGCTG CCTGGTTTCC CAGGTTTTCC CGGAGAAAGA 
GGAAAGCCTG GTGCAGAGGG ATGTCCTGGC ACAAAGGGAG AACCTGGAGA GAAGGGCATG 
TCTGGCCTTC CCGGAGACCG GGGACTGAGA GGGGCCAAAG GAGCCATAGG ACCTCCCGGA 
GATGAAGGAG AAATGGCTAT CATTTCCCAA AAGGGAACAC CTGGGGAACC TGGACCTCCT 
GGAGATGATG GATTCCCAGG AGAAAGAGGT GATAAAGGAA CTCCCGGGAT GCAAGGGAGA 
AGAGGAGAGC CGGGAAGATA CGGACCACCT GGATTTCAGA GAGGGGAACC TGGTGAGAAA 
GGTCAGCCAG GGCATCCTGG ACCCCCAGGC CCTCCAGGCT CAACTGGTCT AAGAGGGTTC 
ATTGGTTTTC CAGGACTTCC AGGTGACCAG GGTGAGCCAG GTTCTCCAGG TCCCCCTGGA 
TTTTCAGGAA TTGATGGAGC AAGAGGACCT AAAGGAAACA AAGGTGACCC TGCCAGTCAC 
TTTGGTCCAC CTGGTCCAAA GGGTGAGCCA GGTAGCCCTG GATGTCCAGG GCATTTTGGA 
GCATCTGGAG AGCAGGGCTT GCCTGGTATT CAAGGGCCCA GAGGATCACC TGGAAGGCCA 
GGGCCACCTG GCTCCTCTGG ACCACCAGGG TGCCCAGGTG ATCACGGGAT GCCTGGGCTG 
AGGGGACAGC CAGGAGAAAT GGGAGACCCT GGGCCAAGAG GCCTCCAGGG GGATCCAGGG 
ATACCAGGTC CTCCGGGAAT AAAAGGTCCC TCCGGATCAC CTGGCCTGAA CGGCTTGCAT 
GGATTGAAAG GTCAGAAAGG AACTAAAGGT GCTTCAGGTT TGCATGATGT GGGGCCACCT 
GGTCCAGTGG GAATACCTGG GCTAAAAGGG GAGAGAGGAG ACCCTGGGAG CCCAGGAATC 
TCTCCTCCAG GTCCTCGTGG AGAAAAAGGT CCCCCAGGAC CCCCAGGGAG TTCAGGACCA 
CCTGGTCCTG CAGGTGCCAC AGGAAGAGCT CCTAAGGACA TTCCTGACCC GGGTCCACCT 
GGAGATCAGG GACCTCCTGG TCCTGATGGC CCAAGAGGAG CACCTGGGCC TCCAGGCCTC 
CCTGGGAGTG TTGACCTTCT GAGAGGGGAG CCAGGTGACT GTGGTCTACC AGGGCCACCA 
GGTCCCCCTG GCCCACCAGG CCCTCCAGGA TACAAAGGCT TTCCAGGATG TGATGGAAAA 
GATGGCCAGA AAGGACCAAT GGGATTCCCG GGACCGCAGG GACCACATGG ATTTCCTGGG 
CCACCTGGAG AGAAGGGTTT ACCTGGACCT CCAGGGAGAA AAGGGCCCAC TGGTCTTCCA 
GGTCCCAGAG GTGAACCGGG GCCACCTGCA GATGTGGATG ACTGTCCCCG AATCCCAGGC 
CTTCCTGGGG CGCCAGGCAT GAGAGGACCA GAAGGAGCCA TGGGGCTCTC TGGAATGAGA 
GGCCCCCCAG GACCAGGGTG CAAAGGAGAG CCTGGGCTGG ATGGCAGGAG GGGTGTGGAT 
GGCGTCCCTG GGTCTCCTGG GCCTCCCGGA CGTAAAGGTG ACACAGGAGA AGACGGCTAC 
CCTGGAGGAC CAGGGCCTCC TGGTCCCATT GGGGATCCTG GGCCCAAAGG GTTTGGCCCT 
GGATACCTCG GTGGCTTCCT CCTGGTTCTC CACAGTCAGA CGGACCAGGA GCCCACCTGC 
CCCCTGGGCA TGCCCAGGCT CTGGACTGGG TATAGTCTGT TATACCTGGA AGGGCAAGAG 
AAAGCTCACA ATCAAGACCT TGGTCTGGCA GGGTCTTGCC TTCCCGTGTT TAGCACGCTG 
CCCTTTGCCT ACTGCAACAT CCACCAGGTG TGCCACTATG CCCAGAGAAA CGACAGATCC 
TACTGGCTGG CCAGCGCTGC GCCCCTCCCC ATGATGCCAC TCTCTGAAGA GGCGATCCGC 
CCCTATGTCA GCCGCTGTGC GGTATGCGAG GCCCCGGCCC AGGCGGTGGC GGTGCACAGC 
CAGGACAAGT CCATCCCCCC ATGTCCGCAG ACCTGGAGGA GCCTCTGGAT CGGGTATTCA 
TTCCTGATGC ACACAGGAGC TGGGGACCAA GGAGGAGGGC AGGCCCTTAT GTCACCTGGC 
AGCTGCCTGG AAGATTTCAG AGCAGCACCA TTCCTTGAAT GCCAGGGCCG GCAGGGAACT 
TGCCACTTTT TTGCAAATAA GTATAGCTTC TGGCTCACAA CGGTGAAAGC AGACTTGCAG 
TTTTCCTCTG CTCCAGCACC AGACACCTTA AAAGAAAGCC AGGCCCAACG CCAGAAAATC 
AGCCGGTGCC AGGTCTGCGT GAAGTATAGC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_516128.3
CDS	656..5728
Translation	MWSLHIVLMRCSFRLTKSLATDPWSLTLILFSVQYVYGSGKKYVGPCGGRDCSVCHCVPEKGSRGPPGPPGPQGPIGPLGAPGPIGLSGEKGMRGDHGPPGAAGDKGDKGSTGVPGFPGLDGIPGHPGPPGPRGKPGMSGHNGSRGDPGFPGGRGALGPGGPLGHPGEKGEKGNSVFILGAVKGIQGDRGDPGLPGLPGSWGAGGPAGPTGYPGEPGLVGPPGQPGRPGLKGNPGVGVKGQMGDPGEVGQQGSPGPTLLVEPPDFRLYKGEKGIKGIPGMVGLPGPPGRKGESDIGAKGEKGIPGFPGPRGDPGSYGSPGFPGLKGELGLVGDPGLFGLIGPKGDPGNRGHPGPPGVLVTPPLPLKGPPGDPGFPGRYGETGDVGPPGPPGLLGRPGEACAGMIGPPGPQGFPGLPGLPGEAGIPGRPDSAPGKPGKPGSPGLPGAPGLQGLPGSSVIYCSVGNPGPQGIKGKVGPPGGRGSKGEKGNEGLCACEPGPMGPPGPPGLPGRQGSKGDLGLPGWLGTKGDPGPPGAEGPPGLPGKHGASGPPGNKGAKGDMVVSRVKGHKGERGPDGPPGFPGQPGSHGRDGHAGEKGDPGPPGDHEDATPGGKGFPGPLGPPGKAGPLGPPGLGFPGPPGQRGHPGVPGHPGVRGPDGLKGQKGDTISCNVTYPGRHGPPGFDGPPGPKGFPGPQGAPGLSGSDGHKGRPGTPGTAEIPGPPGFRGDMGDPGFGGEKGSSPAGPPGPPGSPGVNGQKGIPGDPAFGHLGPPGKRGLSGVPGIKGPRGDPGCPGAEGPAGIPGFPGLKGPKGREGHAGFPGVPGPPGHSCERGAPGIPGQPGLPGYPGSPGAPGGKGQLGDVGPPGPAGMKGLPGLPGWPGAHGPPGLPGIPGPFGDDGLPGPPGPKGPQGLPGFPGFPGERGKPGAEGCPGTKGEPGEKGMSGLPGDRGLRGAKGAIGPPGDEGEMAIISQKGTPGEPGPPGDDGFPGERGDKGTPGMQGRRGEPGRYGPPGFQRGEPGEKGQPGHPGPPGPPGSTGLRGFIGFPGLPGDQGEPGSPGPPGFSGIDGARGPKGNKGDPASHFGPPGPKGEPGSPGCPGHFGASGEQGLPGIQGPRGSPGRPGPPGSSGPPGCPGDHGMPGLRGQPGEMGDPGPRGLQGDPGIPGPPGIKGPSGSPGLNGLHGLKGQKGTKGASGLHDVGPPGPVGIPGLKGERGDPGSPGISPPGPRGEKGPPGPPGSSGPPGPAGATGRAPKDIPDPGPPGDQGPPGPDGPRGAPGPPGLPGSVDLLRGEPGDCGLPGPPGPPGPPGPPGYKGFPGCDGKDGQKGPMGFPGPQGPHGFPGPPGEKGLPGPPGRKGPTGLPGPRGEPGPPADVDDCPRIPGLPGAPGMRGPEGAMGLSGMRGPPGPGCKGEPGLDGRRGVDGVPGSPGPPGRKGDTGEDGYPGGPGPPGPIGDPGPKGFGPGYLGGFLLVLHSQTDQEPTCPLGMPRLWTGYSLLYLEGQEKAHNQDLGLAGSCLPVFSTLPFAYCNIHQVCHYAQRNDRSYWLASAAPLPMMPLSEEAIRPYVSRCAVCEAPAQAVAVHSQDKSIPPCPQTWRSLWIGYSFLMHTGAGDQGGGQALMSPGSCLEDFRAAPFLECQGRQGTCHFFANKYSFWLTTVKADLQFSSAPAPDTLKESQAQRQKISRCQVCVKYS

Target ORF information:

RefSeq Version	XM_516128.4
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes collagen type IV alpha 4 (COL4A4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_516128.4

ORF Insert Sequence:

ATGTGGTCTC TGCACATAGT ACTAATGAGG TGCTCCTTCA GATTGACCAA GTCCTTGGCC 
ACAGATCCCT GGTCACTTAC ACTCATTCTC TTTTCTGTAC AATATGTATA TGGGAGTGGA 
AAGAAATACG TTGGTCCTTG TGGAGGAAGA GATTGCTCTG TTTGCCACTG TGTTCCTGAA 
AAGGGGTCTC GGGGTCCACC AGGACCACCA GGGCCACAGG GTCCAATTGG ACCCCTGGGA 
GCCCCAGGAC CCATTGGGCT TTCAGGAGAG AAAGGAATGA GAGGGGACCA CGGCCCTCCT 
GGAGCAGCAG GGGACAAAGG AGATAAGGGT TCAACTGGTG TTCCTGGATT TCCAGGTTTA 
GATGGCATAC CTGGGCACCC AGGGCCTCCT GGACCCAGAG GCAAACCTGG TATGAGTGGC 
CACAATGGCT CAAGAGGTGA CCCAGGGTTT CCAGGAGGAA GAGGAGCTCT TGGCCCAGGA 
GGCCCCCTAG GCCATCCTGG GGAAAAGGGA GAAAAAGGAA ATTCAGTGTT CATTTTAGGT 
GCCGTTAAAG GTATTCAGGG AGACAGAGGG GACCCAGGAC TGCCTGGCTT ACCAGGATCT 
TGGGGGGCAG GAGGACCGGC GGGTCCCACA GGATATCCTG GAGAGCCAGG GTTAGTGGGA 
CCTCCGGGCC AACCAGGGCG TCCAGGTTTG AAGGGAAATC CCGGTGTGGG AGTAAAGGGG 
CAAATGGGAG ACCCGGGTGA GGTTGGTCAG CAAGGTTCTC CTGGACCCAC CCTGTTGGTG 
GAGCCACCTG ACTTTCGTCT CTATAAAGGA GAAAAGGGTA TAAAAGGAAT TCCTGGAATG 
GTTGGACTGC CAGGACCACC AGGACGCAAG GGAGAATCTG ATATTGGGGC AAAAGGAGAA 
AAAGGTATTC CTGGATTTCC AGGGCCTCGG GGGGATCCTG GTTCCTATGG ATCTCCAGGT 
TTTCCAGGAT TAAAGGGAGA ACTAGGACTG GTTGGAGATC CTGGGCTATT TGGATTAATT 
GGCCCAAAGG GGGATCCTGG AAATCGAGGG CACCCAGGAC CACCAGGTGT TTTGGTGACT 
CCACCTCTTC CACTCAAAGG CCCACCAGGG GACCCAGGGT TCCCTGGCCG CTATGGAGAA 
ACAGGGGATG TTGGACCACC TGGTCCCCCA GGTCTCTTGG GCAGACCAGG GGAAGCCTGT 
GCAGGCATGA TAGGACCCCC TGGGCCACAA GGATTTCCTG GTCTTCCTGG GCTTCCAGGA 
GAAGCTGGTA TTCCTGGGAG ACCTGATTCT GCTCCAGGAA AACCAGGGAA GCCAGGATCA 
CCTGGCTTGC CTGGAGCACC AGGCCTGCAG GGCCTCCCAG GATCAAGTGT GATATACTGT 
AGTGTTGGGA ATCCTGGACC ACAAGGAATA AAAGGCAAAG TGGGTCCCCC AGGAGGAAGA 
GGCTCAAAAG GAGAAAAAGG AAATGAAGGA CTCTGTGCCT GTGAGCCTGG ACCCATGGGC 
CCCCCTGGCC CTCCAGGACT TCCTGGGAGG CAGGGGAGTA AGGGAGACTT GGGGCTCCCT 
GGCTGGCTTG GAACAAAAGG TGACCCAGGA CCTCCTGGTG CTGAAGGACC TCCAGGGCTA 
CCAGGAAAGC ATGGTGCCTC TGGACCACCT GGCAACAAAG GGGCGAAGGG TGACATGGTT 
GTATCAAGAG TTAAAGGGCA CAAAGGAGAA AGAGGTCCTG ATGGGCCCCC AGGATTTCCA 
GGGCAGCCAG GATCACATGG TCGGGATGGA CATGCTGGAG AAAAAGGGGA TCCAGGACCC 
CCAGGGGATC ATGAAGATGC GACCCCAGGT GGTAAAGGAT TTCCTGGACC TCTGGGCCCC 
CCGGGCAAAG CAGGACCTTT GGGGCCCCCA GGACTGGGAT TTCCTGGTCC ACCAGGACAG 
CGAGGCCACC CAGGAGTTCC AGGCCACCCA GGTGTGAGGG GCCCTGATGG CTTGAAGGGT 
CAGAAAGGTG ACACAATTTC TTGCAACGTA ACCTACCCTG GGAGGCACGG CCCTCCAGGT 
TTTGATGGAC CTCCAGGTCC GAAGGGATTT CCAGGTCCCC AAGGTGCCCC TGGGCTGAGT 
GGTTCAGATG GGCATAAAGG CAGACCTGGC ACACCAGGAA CAGCGGAAAT ACCAGGTCCA 
CCTGGTTTTC GTGGTGACAT GGGAGATCCG GGTTTTGGAG GTGAAAAGGG GTCCTCCCCT 
GCTGGGCCCC CAGGCCCTCC CGGCTCACCA GGAGTGAATG GTCAGAAAGG AATCCCGGGA 
GACCCTGCAT TTGGTCACCT GGGACCCCCG GGAAAGAGGG GTCTTTCAGG AGTGCCAGGG 
ATAAAAGGAC CCAGAGGTGA TCCGGGATGT CCAGGGGCTG AAGGGCCAGC TGGCATTCCT 
GGATTCCCAG GTCTCAAAGG TCCCAAAGGC AGAGAGGGAC ATGCTGGGTT TCCAGGTGTC 
CCAGGTCCAC CTGGCCATTC CTGTGAAAGA GGTGCTCCAG GGATACCAGG GCAACCGGGA 
CTCCCTGGGT ATCCAGGTAG CCCAGGTGCT CCAGGTGGGA AAGGACAGCT GGGAGATGTG 
GGGCCTCCCG GGCCAGCTGG AATGAAAGGC CTCCCTGGAC TCCCAGGATG GCCTGGGGCA 
CATGGTCCCC CAGGCCTCCC AGGAATCCCA GGTCCCTTTG GAGATGATGG GCTACCTGGT 
CCTCCAGGTC CAAAGGGACC CCAGGGGCTG CCTGGTTTCC CAGGTTTTCC CGGAGAAAGA 
GGAAAGCCTG GTGCAGAGGG ATGTCCTGGC ACAAAGGGAG AACCTGGAGA GAAGGGCATG 
TCTGGCCTTC CCGGAGACCG GGGACTGAGA GGGGCCAAAG GAGCCATAGG ACCTCCCGGA 
GATGAAGGAG AAATGGCTAT CATTTCCCAA AAGGGAACAC CTGGGGAACC TGGACCTCCT 
GGAGATGATG GATTCCCAGG AGAAAGAGGT GATAAAGGAA CTCCCGGGAT GCAAGGGAGA 
AGAGGAGAGC CGGGAAGATA CGGACCACCT GGATTTCAGA GAGGGGAACC TGGTGAGAAA 
GGTCAGCCAG GGCATCCTGG ACCCCCAGGC CCTCCAGGCT CAACTGGTCT AAGAGGGTTC 
ATTGGTTTTC CAGGACTTCC AGGTGACCAG GGTGAGCCAG GTTCTCCAGG TCCCCCTGGA 
TTTTCAGGAA TTGATGGAGC AAGAGGACCT AAAGGAAACA AAGGTGACCC TGCCAGTCAC 
TTTGGTCCAC CTGGTCCAAA GGGTGAGCCA GGTAGCCCTG GATGTCCAGG GCATTTTGGA 
GCATCTGGAG AGCAGGGCTT GCCTGGTATT CAAGGGCCCA GAGGATCACC TGGAAGGCCA 
GGGCCACCTG GCTCCTCTGG ACCACCAGGG TGCCCAGGTG ATCACGGGAT GCCTGGGCTG 
AGGGGACAGC CAGGAGAAAT GGGAGACCCT GGGCCAAGAG GCCTCCAGGG GGATCCAGGG 
ATACCAGGTC CTCCGGGAAT AAAAGGTCCC TCCGGATCAC CTGGCCTGAA CGGCTTGCAT 
GGATTGAAAG GTCAGAAAGG AACTAAAGGT GCTTCAGGTT TGCATGATGT GGGGCCACCT 
GGTCCAGTGG GAATACCTGG GCTAAAAGGG GAGAGAGGAG ACCCTGGGAG CCCAGGAATC 
TCTCCTCCAG GTCCTCGTGG AGAAAAAGGT CCCCCAGGAC CCCCAGGGAG TTCAGGACCA 
CCTGGTCCTG CAGGTGCCAC AGGAAGAGCT CCTAAGGACA TTCCTGACCC GGGTCCACCT 
GGAGATCAGG GACCTCCTGG TCCTGATGGC CCAAGAGGAG CACCTGGGCC TCCAGGCCTC 
CCTGGGAGTG TTGACCTTCT GAGAGGGGAG CCAGGTGACT GTGGTCTACC AGGGCCACCA 
GGTCCCCCTG GCCCACCAGG CCCTCCAGGA TACAAAGGCT TTCCAGGATG TGATGGAAAA 
GATGGCCAGA AAGGACCAAT GGGATTCCCG GGACCGCAGG GACCACATGG ATTTCCTGGG 
CCACCTGGAG AGAAGGGTTT ACCTGGACCT CCAGGGAGAA AAGGGCCCAC TGGTCTTCCA 
GGTCCCAGAG GTGAACCGGG GCCACCTGCA GATGTGGATG ACTGTCCCCG AATCCCAGGC 
CTTCCTGGGG CGCCAGGCAT GAGAGGACCA GAAGGAGCCA TGGGGCTCTC TGGAATGAGA 
GGCCCCCCAG GACCAGGGTG CAAAGGAGAG CCTGGGCTGG ATGGCAGGAG GGGTGTGGAT 
GGCGTCCCTG GGTCTCCTGG GCCTCCCGGA CGTAAAGGTG ACACAGGAGA AGACGGCTAC 
CCTGGAGGAC CAGGGCCTCC TGGTCCCATT GGGGATCCTG GGCCCAAAGG GTTTGGCCCT 
GGATACCTCG GTGGCTTCCT CCTGGTTCTC CACAGTCAGA CGGACCAGGA GCCCACCTGC 
CCCCTGGGCA TGCCCAGGCT CTGGACTGGG TATAGTCTGT TATACCTGGA AGGGCAAGAG 
AAAGCTCACA ATCAAGACCT TGGTCTGGCA GGGTCTTGCC TTCCCGTGTT TAGCACGCTG 
CCCTTTGCCT ACTGCAACAT CCACCAGGTG TGCCACTATG CCCAGAGAAA CGACAGATCC 
TACTGGCTGG CCAGCGCTGC GCCCCTCCCC ATGATGCCAC TCTCTGAAGA GGCGATCCGC 
CCCTATGTCA GCCGCTGTGC GGTATGCGAG GCCCCGGCCC AGGCGGTGGC GGTGCACAGC 
CAGGACAAGT CCATCCCCCC ATGTCCGCAG ACCTGGAGGA GCCTCTGGAT CGGGTATTCA 
TTCCTGATGC ACACAGGAGC TGGGGACCAA GGAGGAGGGC AGGCCCTTAT GTCACCTGGC 
AGCTGCCTGG AAGATTTCAG AGCAGCACCA TTCCTTGAAT GCCAGGGCCG GCAGGGAACT 
TGCCACTTTT TTGCAAATAA GTATAGCTTC TGGCTCACAA CGGTGAAAGC AGACTTGCAG 
TTTTCCTCTG CTCCAGCACC AGACACCTTA AAAGAAAGCC AGGCCCAACG CCAGAAAATC 
AGCCGGTGCC AGGTCTGCGT GAAGTATAGC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL4A4 ( XM_016950591.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016806080.1 Pan troglodytes collagen type IV alpha 4 (COL4A4), transcript variant X3, mRNA.

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CloneID	OPc80763
Clone ID Related Accession (Same CDS sequence)	XM_009444465.2 , XM_516128.4 , XM_016950591.1 , XM_009444464.2
Accession Version	XM_016950591.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5073bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	collagen alpha-4(IV) chain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006470.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGTCTC TGCACATAGT ACTAATGAGG TGCTCCTTCA GATTGACCAA GTCCTTGGCC 
ACAGATCCCT GGTCACTTAC ACTCATTCTC TTTTCTGTAC AATATGTATA TGGGAGTGGA 
AAGAAATACG TTGGTCCTTG TGGAGGAAGA GATTGCTCTG TTTGCCACTG TGTTCCTGAA 
AAGGGGTCTC GGGGTCCACC AGGACCACCA GGGCCACAGG GTCCAATTGG ACCCCTGGGA 
GCCCCAGGAC CCATTGGGCT TTCAGGAGAG AAAGGAATGA GAGGGGACCA CGGCCCTCCT 
GGAGCAGCAG GGGACAAAGG AGATAAGGGT TCAACTGGTG TTCCTGGATT TCCAGGTTTA 
GATGGCATAC CTGGGCACCC AGGGCCTCCT GGACCCAGAG GCAAACCTGG TATGAGTGGC 
CACAATGGCT CAAGAGGTGA CCCAGGGTTT CCAGGAGGAA GAGGAGCTCT TGGCCCAGGA 
GGCCCCCTAG GCCATCCTGG GGAAAAGGGA GAAAAAGGAA ATTCAGTGTT CATTTTAGGT 
GCCGTTAAAG GTATTCAGGG AGACAGAGGG GACCCAGGAC TGCCTGGCTT ACCAGGATCT 
TGGGGGGCAG GAGGACCGGC GGGTCCCACA GGATATCCTG GAGAGCCAGG GTTAGTGGGA 
CCTCCGGGCC AACCAGGGCG TCCAGGTTTG AAGGGAAATC CCGGTGTGGG AGTAAAGGGG 
CAAATGGGAG ACCCGGGTGA GGTTGGTCAG CAAGGTTCTC CTGGACCCAC CCTGTTGGTG 
GAGCCACCTG ACTTTCGTCT CTATAAAGGA GAAAAGGGTA TAAAAGGAAT TCCTGGAATG 
GTTGGACTGC CAGGACCACC AGGACGCAAG GGAGAATCTG ATATTGGGGC AAAAGGAGAA 
AAAGGTATTC CTGGATTTCC AGGGCCTCGG GGGGATCCTG GTTCCTATGG ATCTCCAGGT 
TTTCCAGGAT TAAAGGGAGA ACTAGGACTG GTTGGAGATC CTGGGCTATT TGGATTAATT 
GGCCCAAAGG GGGATCCTGG AAATCGAGGG CACCCAGGAC CACCAGGTGT TTTGGTGACT 
CCACCTCTTC CACTCAAAGG CCCACCAGGG GACCCAGGGT TCCCTGGCCG CTATGGAGAA 
ACAGGGGATG TTGGACCACC TGGTCCCCCA GGTCTCTTGG GCAGACCAGG GGAAGCCTGT 
GCAGGCATGA TAGGACCCCC TGGGCCACAA GGATTTCCTG GTCTTCCTGG GCTTCCAGGA 
GAAGCTGGTA TTCCTGGGAG ACCTGATTCT GCTCCAGGAA AACCAGGGAA GCCAGGATCA 
CCTGGCTTGC CTGGAGCACC AGGCCTGCAG GGCCTCCCAG GATCAAGTGT GATATACTGT 
AGTGTTGGGA ATCCTGGACC ACAAGGAATA AAAGGCAAAG TGGGTCCCCC AGGAGGAAGA 
GGCTCAAAAG GAGAAAAAGG AAATGAAGGA CTCTGTGCCT GTGAGCCTGG ACCCATGGGC 
CCCCCTGGCC CTCCAGGACT TCCTGGGAGG CAGGGGAGTA AGGGAGACTT GGGGCTCCCT 
GGCTGGCTTG GAACAAAAGG TGACCCAGGA CCTCCTGGTG CTGAAGGACC TCCAGGGCTA 
CCAGGAAAGC ATGGTGCCTC TGGACCACCT GGCAACAAAG GGGCGAAGGG TGACATGGTT 
GTATCAAGAG TTAAAGGGCA CAAAGGAGAA AGAGGTCCTG ATGGGCCCCC AGGATTTCCA 
GGGCAGCCAG GATCACATGG TCGGGATGGA CATGCTGGAG AAAAAGGGGA TCCAGGACCC 
CCAGGGGATC ATGAAGATGC GACCCCAGGT GGTAAAGGAT TTCCTGGACC TCTGGGCCCC 
CCGGGCAAAG CAGGACCTTT GGGGCCCCCA GGACTGGGAT TTCCTGGTCC ACCAGGACAG 
CGAGGCCACC CAGGAGTTCC AGGCCACCCA GGTGTGAGGG GCCCTGATGG CTTGAAGGGT 
CAGAAAGGTG ACACAATTTC TTGCAACGTA ACCTACCCTG GGAGGCACGG CCCTCCAGGT 
TTTGATGGAC CTCCAGGTCC GAAGGGATTT CCAGGTCCCC AAGGTGCCCC TGGGCTGAGT 
GGTTCAGATG GGCATAAAGG CAGACCTGGC ACACCAGGAA CAGCGGAAAT ACCAGGTCCA 
CCTGGTTTTC GTGGTGACAT GGGAGATCCG GGTTTTGGAG GTGAAAAGGG GTCCTCCCCT 
GCTGGGCCCC CAGGCCCTCC CGGCTCACCA GGAGTGAATG GTCAGAAAGG AATCCCGGGA 
GACCCTGCAT TTGGTCACCT GGGACCCCCG GGAAAGAGGG GTCTTTCAGG AGTGCCAGGG 
ATAAAAGGAC CCAGAGGTGA TCCGGGATGT CCAGGGGCTG AAGGGCCAGC TGGCATTCCT 
GGATTCCCAG GTCTCAAAGG TCCCAAAGGC AGAGAGGGAC ATGCTGGGTT TCCAGGTGTC 
CCAGGTCCAC CTGGCCATTC CTGTGAAAGA GGTGCTCCAG GGATACCAGG GCAACCGGGA 
CTCCCTGGGT ATCCAGGTAG CCCAGGTGCT CCAGGTGGGA AAGGACAGCT GGGAGATGTG 
GGGCCTCCCG GGCCAGCTGG AATGAAAGGC CTCCCTGGAC TCCCAGGATG GCCTGGGGCA 
CATGGTCCCC CAGGCCTCCC AGGAATCCCA GGTCCCTTTG GAGATGATGG GCTACCTGGT 
CCTCCAGGTC CAAAGGGACC CCAGGGGCTG CCTGGTTTCC CAGGTTTTCC CGGAGAAAGA 
GGAAAGCCTG GTGCAGAGGG ATGTCCTGGC ACAAAGGGAG AACCTGGAGA GAAGGGCATG 
TCTGGCCTTC CCGGAGACCG GGGACTGAGA GGGGCCAAAG GAGCCATAGG ACCTCCCGGA 
GATGAAGGAG AAATGGCTAT CATTTCCCAA AAGGGAACAC CTGGGGAACC TGGACCTCCT 
GGAGATGATG GATTCCCAGG AGAAAGAGGT GATAAAGGAA CTCCCGGGAT GCAAGGGAGA 
AGAGGAGAGC CGGGAAGATA CGGACCACCT GGATTTCAGA GAGGGGAACC TGGTGAGAAA 
GGTCAGCCAG GGCATCCTGG ACCCCCAGGC CCTCCAGGCT CAACTGGTCT AAGAGGGTTC 
ATTGGTTTTC CAGGACTTCC AGGTGACCAG GGTGAGCCAG GTTCTCCAGG TCCCCCTGGA 
TTTTCAGGAA TTGATGGAGC AAGAGGACCT AAAGGAAACA AAGGTGACCC TGCCAGTCAC 
TTTGGTCCAC CTGGTCCAAA GGGTGAGCCA GGTAGCCCTG GATGTCCAGG GCATTTTGGA 
GCATCTGGAG AGCAGGGCTT GCCTGGTATT CAAGGGCCCA GAGGATCACC TGGAAGGCCA 
GGGCCACCTG GCTCCTCTGG ACCACCAGGG TGCCCAGGTG ATCACGGGAT GCCTGGGCTG 
AGGGGACAGC CAGGAGAAAT GGGAGACCCT GGGCCAAGAG GCCTCCAGGG GGATCCAGGG 
ATACCAGGTC CTCCGGGAAT AAAAGGTCCC TCCGGATCAC CTGGCCTGAA CGGCTTGCAT 
GGATTGAAAG GTCAGAAAGG AACTAAAGGT GCTTCAGGTT TGCATGATGT GGGGCCACCT 
GGTCCAGTGG GAATACCTGG GCTAAAAGGG GAGAGAGGAG ACCCTGGGAG CCCAGGAATC 
TCTCCTCCAG GTCCTCGTGG AGAAAAAGGT CCCCCAGGAC CCCCAGGGAG TTCAGGACCA 
CCTGGTCCTG CAGGTGCCAC AGGAAGAGCT CCTAAGGACA TTCCTGACCC GGGTCCACCT 
GGAGATCAGG GACCTCCTGG TCCTGATGGC CCAAGAGGAG CACCTGGGCC TCCAGGCCTC 
CCTGGGAGTG TTGACCTTCT GAGAGGGGAG CCAGGTGACT GTGGTCTACC AGGGCCACCA 
GGTCCCCCTG GCCCACCAGG CCCTCCAGGA TACAAAGGCT TTCCAGGATG TGATGGAAAA 
GATGGCCAGA AAGGACCAAT GGGATTCCCG GGACCGCAGG GACCACATGG ATTTCCTGGG 
CCACCTGGAG AGAAGGGTTT ACCTGGACCT CCAGGGAGAA AAGGGCCCAC TGGTCTTCCA 
GGTCCCAGAG GTGAACCGGG GCCACCTGCA GATGTGGATG ACTGTCCCCG AATCCCAGGC 
CTTCCTGGGG CGCCAGGCAT GAGAGGACCA GAAGGAGCCA TGGGGCTCTC TGGAATGAGA 
GGCCCCCCAG GACCAGGGTG CAAAGGAGAG CCTGGGCTGG ATGGCAGGAG GGGTGTGGAT 
GGCGTCCCTG GGTCTCCTGG GCCTCCCGGA CGTAAAGGTG ACACAGGAGA AGACGGCTAC 
CCTGGAGGAC CAGGGCCTCC TGGTCCCATT GGGGATCCTG GGCCCAAAGG GTTTGGCCCT 
GGATACCTCG GTGGCTTCCT CCTGGTTCTC CACAGTCAGA CGGACCAGGA GCCCACCTGC 
CCCCTGGGCA TGCCCAGGCT CTGGACTGGG TATAGTCTGT TATACCTGGA AGGGCAAGAG 
AAAGCTCACA ATCAAGACCT TGGTCTGGCA GGGTCTTGCC TTCCCGTGTT TAGCACGCTG 
CCCTTTGCCT ACTGCAACAT CCACCAGGTG TGCCACTATG CCCAGAGAAA CGACAGATCC 
TACTGGCTGG CCAGCGCTGC GCCCCTCCCC ATGATGCCAC TCTCTGAAGA GGCGATCCGC 
CCCTATGTCA GCCGCTGTGC GGTATGCGAG GCCCCGGCCC AGGCGGTGGC GGTGCACAGC 
CAGGACAAGT CCATCCCCCC ATGTCCGCAG ACCTGGAGGA GCCTCTGGAT CGGGTATTCA 
TTCCTGATGC ACACAGGAGC TGGGGACCAA GGAGGAGGGC AGGCCCTTAT GTCACCTGGC 
AGCTGCCTGG AAGATTTCAG AGCAGCACCA TTCCTTGAAT GCCAGGGCCG GCAGGGAACT 
TGCCACTTTT TTGCAAATAA GTATAGCTTC TGGCTCACAA CGGTGAAAGC AGACTTGCAG 
TTTTCCTCTG CTCCAGCACC AGACACCTTA AAAGAAAGCC AGGCCCAACG CCAGAAAATC 
AGCCGGTGCC AGGTCTGCGT GAAGTATAGC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016806080.1
CDS	523..5595
Translation	MWSLHIVLMRCSFRLTKSLATDPWSLTLILFSVQYVYGSGKKYVGPCGGRDCSVCHCVPEKGSRGPPGPPGPQGPIGPLGAPGPIGLSGEKGMRGDHGPPGAAGDKGDKGSTGVPGFPGLDGIPGHPGPPGPRGKPGMSGHNGSRGDPGFPGGRGALGPGGPLGHPGEKGEKGNSVFILGAVKGIQGDRGDPGLPGLPGSWGAGGPAGPTGYPGEPGLVGPPGQPGRPGLKGNPGVGVKGQMGDPGEVGQQGSPGPTLLVEPPDFRLYKGEKGIKGIPGMVGLPGPPGRKGESDIGAKGEKGIPGFPGPRGDPGSYGSPGFPGLKGELGLVGDPGLFGLIGPKGDPGNRGHPGPPGVLVTPPLPLKGPPGDPGFPGRYGETGDVGPPGPPGLLGRPGEACAGMIGPPGPQGFPGLPGLPGEAGIPGRPDSAPGKPGKPGSPGLPGAPGLQGLPGSSVIYCSVGNPGPQGIKGKVGPPGGRGSKGEKGNEGLCACEPGPMGPPGPPGLPGRQGSKGDLGLPGWLGTKGDPGPPGAEGPPGLPGKHGASGPPGNKGAKGDMVVSRVKGHKGERGPDGPPGFPGQPGSHGRDGHAGEKGDPGPPGDHEDATPGGKGFPGPLGPPGKAGPLGPPGLGFPGPPGQRGHPGVPGHPGVRGPDGLKGQKGDTISCNVTYPGRHGPPGFDGPPGPKGFPGPQGAPGLSGSDGHKGRPGTPGTAEIPGPPGFRGDMGDPGFGGEKGSSPAGPPGPPGSPGVNGQKGIPGDPAFGHLGPPGKRGLSGVPGIKGPRGDPGCPGAEGPAGIPGFPGLKGPKGREGHAGFPGVPGPPGHSCERGAPGIPGQPGLPGYPGSPGAPGGKGQLGDVGPPGPAGMKGLPGLPGWPGAHGPPGLPGIPGPFGDDGLPGPPGPKGPQGLPGFPGFPGERGKPGAEGCPGTKGEPGEKGMSGLPGDRGLRGAKGAIGPPGDEGEMAIISQKGTPGEPGPPGDDGFPGERGDKGTPGMQGRRGEPGRYGPPGFQRGEPGEKGQPGHPGPPGPPGSTGLRGFIGFPGLPGDQGEPGSPGPPGFSGIDGARGPKGNKGDPASHFGPPGPKGEPGSPGCPGHFGASGEQGLPGIQGPRGSPGRPGPPGSSGPPGCPGDHGMPGLRGQPGEMGDPGPRGLQGDPGIPGPPGIKGPSGSPGLNGLHGLKGQKGTKGASGLHDVGPPGPVGIPGLKGERGDPGSPGISPPGPRGEKGPPGPPGSSGPPGPAGATGRAPKDIPDPGPPGDQGPPGPDGPRGAPGPPGLPGSVDLLRGEPGDCGLPGPPGPPGPPGPPGYKGFPGCDGKDGQKGPMGFPGPQGPHGFPGPPGEKGLPGPPGRKGPTGLPGPRGEPGPPADVDDCPRIPGLPGAPGMRGPEGAMGLSGMRGPPGPGCKGEPGLDGRRGVDGVPGSPGPPGRKGDTGEDGYPGGPGPPGPIGDPGPKGFGPGYLGGFLLVLHSQTDQEPTCPLGMPRLWTGYSLLYLEGQEKAHNQDLGLAGSCLPVFSTLPFAYCNIHQVCHYAQRNDRSYWLASAAPLPMMPLSEEAIRPYVSRCAVCEAPAQAVAVHSQDKSIPPCPQTWRSLWIGYSFLMHTGAGDQGGGQALMSPGSCLEDFRAAPFLECQGRQGTCHFFANKYSFWLTTVKADLQFSSAPAPDTLKESQAQRQKISRCQVCVKYS

Target ORF information:

RefSeq Version	XM_016950591.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes collagen type IV alpha 4 (COL4A4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016950591.1

ORF Insert Sequence:

ATGTGGTCTC TGCACATAGT ACTAATGAGG TGCTCCTTCA GATTGACCAA GTCCTTGGCC 
ACAGATCCCT GGTCACTTAC ACTCATTCTC TTTTCTGTAC AATATGTATA TGGGAGTGGA 
AAGAAATACG TTGGTCCTTG TGGAGGAAGA GATTGCTCTG TTTGCCACTG TGTTCCTGAA 
AAGGGGTCTC GGGGTCCACC AGGACCACCA GGGCCACAGG GTCCAATTGG ACCCCTGGGA 
GCCCCAGGAC CCATTGGGCT TTCAGGAGAG AAAGGAATGA GAGGGGACCA CGGCCCTCCT 
GGAGCAGCAG GGGACAAAGG AGATAAGGGT TCAACTGGTG TTCCTGGATT TCCAGGTTTA 
GATGGCATAC CTGGGCACCC AGGGCCTCCT GGACCCAGAG GCAAACCTGG TATGAGTGGC 
CACAATGGCT CAAGAGGTGA CCCAGGGTTT CCAGGAGGAA GAGGAGCTCT TGGCCCAGGA 
GGCCCCCTAG GCCATCCTGG GGAAAAGGGA GAAAAAGGAA ATTCAGTGTT CATTTTAGGT 
GCCGTTAAAG GTATTCAGGG AGACAGAGGG GACCCAGGAC TGCCTGGCTT ACCAGGATCT 
TGGGGGGCAG GAGGACCGGC GGGTCCCACA GGATATCCTG GAGAGCCAGG GTTAGTGGGA 
CCTCCGGGCC AACCAGGGCG TCCAGGTTTG AAGGGAAATC CCGGTGTGGG AGTAAAGGGG 
CAAATGGGAG ACCCGGGTGA GGTTGGTCAG CAAGGTTCTC CTGGACCCAC CCTGTTGGTG 
GAGCCACCTG ACTTTCGTCT CTATAAAGGA GAAAAGGGTA TAAAAGGAAT TCCTGGAATG 
GTTGGACTGC CAGGACCACC AGGACGCAAG GGAGAATCTG ATATTGGGGC AAAAGGAGAA 
AAAGGTATTC CTGGATTTCC AGGGCCTCGG GGGGATCCTG GTTCCTATGG ATCTCCAGGT 
TTTCCAGGAT TAAAGGGAGA ACTAGGACTG GTTGGAGATC CTGGGCTATT TGGATTAATT 
GGCCCAAAGG GGGATCCTGG AAATCGAGGG CACCCAGGAC CACCAGGTGT TTTGGTGACT 
CCACCTCTTC CACTCAAAGG CCCACCAGGG GACCCAGGGT TCCCTGGCCG CTATGGAGAA 
ACAGGGGATG TTGGACCACC TGGTCCCCCA GGTCTCTTGG GCAGACCAGG GGAAGCCTGT 
GCAGGCATGA TAGGACCCCC TGGGCCACAA GGATTTCCTG GTCTTCCTGG GCTTCCAGGA 
GAAGCTGGTA TTCCTGGGAG ACCTGATTCT GCTCCAGGAA AACCAGGGAA GCCAGGATCA 
CCTGGCTTGC CTGGAGCACC AGGCCTGCAG GGCCTCCCAG GATCAAGTGT GATATACTGT 
AGTGTTGGGA ATCCTGGACC ACAAGGAATA AAAGGCAAAG TGGGTCCCCC AGGAGGAAGA 
GGCTCAAAAG GAGAAAAAGG AAATGAAGGA CTCTGTGCCT GTGAGCCTGG ACCCATGGGC 
CCCCCTGGCC CTCCAGGACT TCCTGGGAGG CAGGGGAGTA AGGGAGACTT GGGGCTCCCT 
GGCTGGCTTG GAACAAAAGG TGACCCAGGA CCTCCTGGTG CTGAAGGACC TCCAGGGCTA 
CCAGGAAAGC ATGGTGCCTC TGGACCACCT GGCAACAAAG GGGCGAAGGG TGACATGGTT 
GTATCAAGAG TTAAAGGGCA CAAAGGAGAA AGAGGTCCTG ATGGGCCCCC AGGATTTCCA 
GGGCAGCCAG GATCACATGG TCGGGATGGA CATGCTGGAG AAAAAGGGGA TCCAGGACCC 
CCAGGGGATC ATGAAGATGC GACCCCAGGT GGTAAAGGAT TTCCTGGACC TCTGGGCCCC 
CCGGGCAAAG CAGGACCTTT GGGGCCCCCA GGACTGGGAT TTCCTGGTCC ACCAGGACAG 
CGAGGCCACC CAGGAGTTCC AGGCCACCCA GGTGTGAGGG GCCCTGATGG CTTGAAGGGT 
CAGAAAGGTG ACACAATTTC TTGCAACGTA ACCTACCCTG GGAGGCACGG CCCTCCAGGT 
TTTGATGGAC CTCCAGGTCC GAAGGGATTT CCAGGTCCCC AAGGTGCCCC TGGGCTGAGT 
GGTTCAGATG GGCATAAAGG CAGACCTGGC ACACCAGGAA CAGCGGAAAT ACCAGGTCCA 
CCTGGTTTTC GTGGTGACAT GGGAGATCCG GGTTTTGGAG GTGAAAAGGG GTCCTCCCCT 
GCTGGGCCCC CAGGCCCTCC CGGCTCACCA GGAGTGAATG GTCAGAAAGG AATCCCGGGA 
GACCCTGCAT TTGGTCACCT GGGACCCCCG GGAAAGAGGG GTCTTTCAGG AGTGCCAGGG 
ATAAAAGGAC CCAGAGGTGA TCCGGGATGT CCAGGGGCTG AAGGGCCAGC TGGCATTCCT 
GGATTCCCAG GTCTCAAAGG TCCCAAAGGC AGAGAGGGAC ATGCTGGGTT TCCAGGTGTC 
CCAGGTCCAC CTGGCCATTC CTGTGAAAGA GGTGCTCCAG GGATACCAGG GCAACCGGGA 
CTCCCTGGGT ATCCAGGTAG CCCAGGTGCT CCAGGTGGGA AAGGACAGCT GGGAGATGTG 
GGGCCTCCCG GGCCAGCTGG AATGAAAGGC CTCCCTGGAC TCCCAGGATG GCCTGGGGCA 
CATGGTCCCC CAGGCCTCCC AGGAATCCCA GGTCCCTTTG GAGATGATGG GCTACCTGGT 
CCTCCAGGTC CAAAGGGACC CCAGGGGCTG CCTGGTTTCC CAGGTTTTCC CGGAGAAAGA 
GGAAAGCCTG GTGCAGAGGG ATGTCCTGGC ACAAAGGGAG AACCTGGAGA GAAGGGCATG 
TCTGGCCTTC CCGGAGACCG GGGACTGAGA GGGGCCAAAG GAGCCATAGG ACCTCCCGGA 
GATGAAGGAG AAATGGCTAT CATTTCCCAA AAGGGAACAC CTGGGGAACC TGGACCTCCT 
GGAGATGATG GATTCCCAGG AGAAAGAGGT GATAAAGGAA CTCCCGGGAT GCAAGGGAGA 
AGAGGAGAGC CGGGAAGATA CGGACCACCT GGATTTCAGA GAGGGGAACC TGGTGAGAAA 
GGTCAGCCAG GGCATCCTGG ACCCCCAGGC CCTCCAGGCT CAACTGGTCT AAGAGGGTTC 
ATTGGTTTTC CAGGACTTCC AGGTGACCAG GGTGAGCCAG GTTCTCCAGG TCCCCCTGGA 
TTTTCAGGAA TTGATGGAGC AAGAGGACCT AAAGGAAACA AAGGTGACCC TGCCAGTCAC 
TTTGGTCCAC CTGGTCCAAA GGGTGAGCCA GGTAGCCCTG GATGTCCAGG GCATTTTGGA 
GCATCTGGAG AGCAGGGCTT GCCTGGTATT CAAGGGCCCA GAGGATCACC TGGAAGGCCA 
GGGCCACCTG GCTCCTCTGG ACCACCAGGG TGCCCAGGTG ATCACGGGAT GCCTGGGCTG 
AGGGGACAGC CAGGAGAAAT GGGAGACCCT GGGCCAAGAG GCCTCCAGGG GGATCCAGGG 
ATACCAGGTC CTCCGGGAAT AAAAGGTCCC TCCGGATCAC CTGGCCTGAA CGGCTTGCAT 
GGATTGAAAG GTCAGAAAGG AACTAAAGGT GCTTCAGGTT TGCATGATGT GGGGCCACCT 
GGTCCAGTGG GAATACCTGG GCTAAAAGGG GAGAGAGGAG ACCCTGGGAG CCCAGGAATC 
TCTCCTCCAG GTCCTCGTGG AGAAAAAGGT CCCCCAGGAC CCCCAGGGAG TTCAGGACCA 
CCTGGTCCTG CAGGTGCCAC AGGAAGAGCT CCTAAGGACA TTCCTGACCC GGGTCCACCT 
GGAGATCAGG GACCTCCTGG TCCTGATGGC CCAAGAGGAG CACCTGGGCC TCCAGGCCTC 
CCTGGGAGTG TTGACCTTCT GAGAGGGGAG CCAGGTGACT GTGGTCTACC AGGGCCACCA 
GGTCCCCCTG GCCCACCAGG CCCTCCAGGA TACAAAGGCT TTCCAGGATG TGATGGAAAA 
GATGGCCAGA AAGGACCAAT GGGATTCCCG GGACCGCAGG GACCACATGG ATTTCCTGGG 
CCACCTGGAG AGAAGGGTTT ACCTGGACCT CCAGGGAGAA AAGGGCCCAC TGGTCTTCCA 
GGTCCCAGAG GTGAACCGGG GCCACCTGCA GATGTGGATG ACTGTCCCCG AATCCCAGGC 
CTTCCTGGGG CGCCAGGCAT GAGAGGACCA GAAGGAGCCA TGGGGCTCTC TGGAATGAGA 
GGCCCCCCAG GACCAGGGTG CAAAGGAGAG CCTGGGCTGG ATGGCAGGAG GGGTGTGGAT 
GGCGTCCCTG GGTCTCCTGG GCCTCCCGGA CGTAAAGGTG ACACAGGAGA AGACGGCTAC 
CCTGGAGGAC CAGGGCCTCC TGGTCCCATT GGGGATCCTG GGCCCAAAGG GTTTGGCCCT 
GGATACCTCG GTGGCTTCCT CCTGGTTCTC CACAGTCAGA CGGACCAGGA GCCCACCTGC 
CCCCTGGGCA TGCCCAGGCT CTGGACTGGG TATAGTCTGT TATACCTGGA AGGGCAAGAG 
AAAGCTCACA ATCAAGACCT TGGTCTGGCA GGGTCTTGCC TTCCCGTGTT TAGCACGCTG 
CCCTTTGCCT ACTGCAACAT CCACCAGGTG TGCCACTATG CCCAGAGAAA CGACAGATCC 
TACTGGCTGG CCAGCGCTGC GCCCCTCCCC ATGATGCCAC TCTCTGAAGA GGCGATCCGC 
CCCTATGTCA GCCGCTGTGC GGTATGCGAG GCCCCGGCCC AGGCGGTGGC GGTGCACAGC 
CAGGACAAGT CCATCCCCCC ATGTCCGCAG ACCTGGAGGA GCCTCTGGAT CGGGTATTCA 
TTCCTGATGC ACACAGGAGC TGGGGACCAA GGAGGAGGGC AGGCCCTTAT GTCACCTGGC 
AGCTGCCTGG AAGATTTCAG AGCAGCACCA TTCCTTGAAT GCCAGGGCCG GCAGGGAACT 
TGCCACTTTT TTGCAAATAA GTATAGCTTC TGGCTCACAA CGGTGAAAGC AGACTTGCAG 
TTTTCCTCTG CTCCAGCACC AGACACCTTA AAAGAAAGCC AGGCCCAACG CCAGAAAATC 
AGCCGGTGCC AGGTCTGCGT GAAGTATAGC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL4A4 ( XM_009444464.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_009442739.2 Pan troglodytes collagen type IV alpha 4 (COL4A4), transcript variant X2, mRNA.

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CloneID	OPc80763
Clone ID Related Accession (Same CDS sequence)	XM_009444465.2 , XM_516128.4 , XM_016950591.1 , XM_009444464.2
Accession Version	XM_009444464.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5073bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	collagen alpha-4(IV) chain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006470.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 2, 2016 this sequence version replaced XM_009444464.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGTCTC TGCACATAGT ACTAATGAGG TGCTCCTTCA GATTGACCAA GTCCTTGGCC 
ACAGATCCCT GGTCACTTAC ACTCATTCTC TTTTCTGTAC AATATGTATA TGGGAGTGGA 
AAGAAATACG TTGGTCCTTG TGGAGGAAGA GATTGCTCTG TTTGCCACTG TGTTCCTGAA 
AAGGGGTCTC GGGGTCCACC AGGACCACCA GGGCCACAGG GTCCAATTGG ACCCCTGGGA 
GCCCCAGGAC CCATTGGGCT TTCAGGAGAG AAAGGAATGA GAGGGGACCA CGGCCCTCCT 
GGAGCAGCAG GGGACAAAGG AGATAAGGGT TCAACTGGTG TTCCTGGATT TCCAGGTTTA 
GATGGCATAC CTGGGCACCC AGGGCCTCCT GGACCCAGAG GCAAACCTGG TATGAGTGGC 
CACAATGGCT CAAGAGGTGA CCCAGGGTTT CCAGGAGGAA GAGGAGCTCT TGGCCCAGGA 
GGCCCCCTAG GCCATCCTGG GGAAAAGGGA GAAAAAGGAA ATTCAGTGTT CATTTTAGGT 
GCCGTTAAAG GTATTCAGGG AGACAGAGGG GACCCAGGAC TGCCTGGCTT ACCAGGATCT 
TGGGGGGCAG GAGGACCGGC GGGTCCCACA GGATATCCTG GAGAGCCAGG GTTAGTGGGA 
CCTCCGGGCC AACCAGGGCG TCCAGGTTTG AAGGGAAATC CCGGTGTGGG AGTAAAGGGG 
CAAATGGGAG ACCCGGGTGA GGTTGGTCAG CAAGGTTCTC CTGGACCCAC CCTGTTGGTG 
GAGCCACCTG ACTTTCGTCT CTATAAAGGA GAAAAGGGTA TAAAAGGAAT TCCTGGAATG 
GTTGGACTGC CAGGACCACC AGGACGCAAG GGAGAATCTG ATATTGGGGC AAAAGGAGAA 
AAAGGTATTC CTGGATTTCC AGGGCCTCGG GGGGATCCTG GTTCCTATGG ATCTCCAGGT 
TTTCCAGGAT TAAAGGGAGA ACTAGGACTG GTTGGAGATC CTGGGCTATT TGGATTAATT 
GGCCCAAAGG GGGATCCTGG AAATCGAGGG CACCCAGGAC CACCAGGTGT TTTGGTGACT 
CCACCTCTTC CACTCAAAGG CCCACCAGGG GACCCAGGGT TCCCTGGCCG CTATGGAGAA 
ACAGGGGATG TTGGACCACC TGGTCCCCCA GGTCTCTTGG GCAGACCAGG GGAAGCCTGT 
GCAGGCATGA TAGGACCCCC TGGGCCACAA GGATTTCCTG GTCTTCCTGG GCTTCCAGGA 
GAAGCTGGTA TTCCTGGGAG ACCTGATTCT GCTCCAGGAA AACCAGGGAA GCCAGGATCA 
CCTGGCTTGC CTGGAGCACC AGGCCTGCAG GGCCTCCCAG GATCAAGTGT GATATACTGT 
AGTGTTGGGA ATCCTGGACC ACAAGGAATA AAAGGCAAAG TGGGTCCCCC AGGAGGAAGA 
GGCTCAAAAG GAGAAAAAGG AAATGAAGGA CTCTGTGCCT GTGAGCCTGG ACCCATGGGC 
CCCCCTGGCC CTCCAGGACT TCCTGGGAGG CAGGGGAGTA AGGGAGACTT GGGGCTCCCT 
GGCTGGCTTG GAACAAAAGG TGACCCAGGA CCTCCTGGTG CTGAAGGACC TCCAGGGCTA 
CCAGGAAAGC ATGGTGCCTC TGGACCACCT GGCAACAAAG GGGCGAAGGG TGACATGGTT 
GTATCAAGAG TTAAAGGGCA CAAAGGAGAA AGAGGTCCTG ATGGGCCCCC AGGATTTCCA 
GGGCAGCCAG GATCACATGG TCGGGATGGA CATGCTGGAG AAAAAGGGGA TCCAGGACCC 
CCAGGGGATC ATGAAGATGC GACCCCAGGT GGTAAAGGAT TTCCTGGACC TCTGGGCCCC 
CCGGGCAAAG CAGGACCTTT GGGGCCCCCA GGACTGGGAT TTCCTGGTCC ACCAGGACAG 
CGAGGCCACC CAGGAGTTCC AGGCCACCCA GGTGTGAGGG GCCCTGATGG CTTGAAGGGT 
CAGAAAGGTG ACACAATTTC TTGCAACGTA ACCTACCCTG GGAGGCACGG CCCTCCAGGT 
TTTGATGGAC CTCCAGGTCC GAAGGGATTT CCAGGTCCCC AAGGTGCCCC TGGGCTGAGT 
GGTTCAGATG GGCATAAAGG CAGACCTGGC ACACCAGGAA CAGCGGAAAT ACCAGGTCCA 
CCTGGTTTTC GTGGTGACAT GGGAGATCCG GGTTTTGGAG GTGAAAAGGG GTCCTCCCCT 
GCTGGGCCCC CAGGCCCTCC CGGCTCACCA GGAGTGAATG GTCAGAAAGG AATCCCGGGA 
GACCCTGCAT TTGGTCACCT GGGACCCCCG GGAAAGAGGG GTCTTTCAGG AGTGCCAGGG 
ATAAAAGGAC CCAGAGGTGA TCCGGGATGT CCAGGGGCTG AAGGGCCAGC TGGCATTCCT 
GGATTCCCAG GTCTCAAAGG TCCCAAAGGC AGAGAGGGAC ATGCTGGGTT TCCAGGTGTC 
CCAGGTCCAC CTGGCCATTC CTGTGAAAGA GGTGCTCCAG GGATACCAGG GCAACCGGGA 
CTCCCTGGGT ATCCAGGTAG CCCAGGTGCT CCAGGTGGGA AAGGACAGCT GGGAGATGTG 
GGGCCTCCCG GGCCAGCTGG AATGAAAGGC CTCCCTGGAC TCCCAGGATG GCCTGGGGCA 
CATGGTCCCC CAGGCCTCCC AGGAATCCCA GGTCCCTTTG GAGATGATGG GCTACCTGGT 
CCTCCAGGTC CAAAGGGACC CCAGGGGCTG CCTGGTTTCC CAGGTTTTCC CGGAGAAAGA 
GGAAAGCCTG GTGCAGAGGG ATGTCCTGGC ACAAAGGGAG AACCTGGAGA GAAGGGCATG 
TCTGGCCTTC CCGGAGACCG GGGACTGAGA GGGGCCAAAG GAGCCATAGG ACCTCCCGGA 
GATGAAGGAG AAATGGCTAT CATTTCCCAA AAGGGAACAC CTGGGGAACC TGGACCTCCT 
GGAGATGATG GATTCCCAGG AGAAAGAGGT GATAAAGGAA CTCCCGGGAT GCAAGGGAGA 
AGAGGAGAGC CGGGAAGATA CGGACCACCT GGATTTCAGA GAGGGGAACC TGGTGAGAAA 
GGTCAGCCAG GGCATCCTGG ACCCCCAGGC CCTCCAGGCT CAACTGGTCT AAGAGGGTTC 
ATTGGTTTTC CAGGACTTCC AGGTGACCAG GGTGAGCCAG GTTCTCCAGG TCCCCCTGGA 
TTTTCAGGAA TTGATGGAGC AAGAGGACCT AAAGGAAACA AAGGTGACCC TGCCAGTCAC 
TTTGGTCCAC CTGGTCCAAA GGGTGAGCCA GGTAGCCCTG GATGTCCAGG GCATTTTGGA 
GCATCTGGAG AGCAGGGCTT GCCTGGTATT CAAGGGCCCA GAGGATCACC TGGAAGGCCA 
GGGCCACCTG GCTCCTCTGG ACCACCAGGG TGCCCAGGTG ATCACGGGAT GCCTGGGCTG 
AGGGGACAGC CAGGAGAAAT GGGAGACCCT GGGCCAAGAG GCCTCCAGGG GGATCCAGGG 
ATACCAGGTC CTCCGGGAAT AAAAGGTCCC TCCGGATCAC CTGGCCTGAA CGGCTTGCAT 
GGATTGAAAG GTCAGAAAGG AACTAAAGGT GCTTCAGGTT TGCATGATGT GGGGCCACCT 
GGTCCAGTGG GAATACCTGG GCTAAAAGGG GAGAGAGGAG ACCCTGGGAG CCCAGGAATC 
TCTCCTCCAG GTCCTCGTGG AGAAAAAGGT CCCCCAGGAC CCCCAGGGAG TTCAGGACCA 
CCTGGTCCTG CAGGTGCCAC AGGAAGAGCT CCTAAGGACA TTCCTGACCC GGGTCCACCT 
GGAGATCAGG GACCTCCTGG TCCTGATGGC CCAAGAGGAG CACCTGGGCC TCCAGGCCTC 
CCTGGGAGTG TTGACCTTCT GAGAGGGGAG CCAGGTGACT GTGGTCTACC AGGGCCACCA 
GGTCCCCCTG GCCCACCAGG CCCTCCAGGA TACAAAGGCT TTCCAGGATG TGATGGAAAA 
GATGGCCAGA AAGGACCAAT GGGATTCCCG GGACCGCAGG GACCACATGG ATTTCCTGGG 
CCACCTGGAG AGAAGGGTTT ACCTGGACCT CCAGGGAGAA AAGGGCCCAC TGGTCTTCCA 
GGTCCCAGAG GTGAACCGGG GCCACCTGCA GATGTGGATG ACTGTCCCCG AATCCCAGGC 
CTTCCTGGGG CGCCAGGCAT GAGAGGACCA GAAGGAGCCA TGGGGCTCTC TGGAATGAGA 
GGCCCCCCAG GACCAGGGTG CAAAGGAGAG CCTGGGCTGG ATGGCAGGAG GGGTGTGGAT 
GGCGTCCCTG GGTCTCCTGG GCCTCCCGGA CGTAAAGGTG ACACAGGAGA AGACGGCTAC 
CCTGGAGGAC CAGGGCCTCC TGGTCCCATT GGGGATCCTG GGCCCAAAGG GTTTGGCCCT 
GGATACCTCG GTGGCTTCCT CCTGGTTCTC CACAGTCAGA CGGACCAGGA GCCCACCTGC 
CCCCTGGGCA TGCCCAGGCT CTGGACTGGG TATAGTCTGT TATACCTGGA AGGGCAAGAG 
AAAGCTCACA ATCAAGACCT TGGTCTGGCA GGGTCTTGCC TTCCCGTGTT TAGCACGCTG 
CCCTTTGCCT ACTGCAACAT CCACCAGGTG TGCCACTATG CCCAGAGAAA CGACAGATCC 
TACTGGCTGG CCAGCGCTGC GCCCCTCCCC ATGATGCCAC TCTCTGAAGA GGCGATCCGC 
CCCTATGTCA GCCGCTGTGC GGTATGCGAG GCCCCGGCCC AGGCGGTGGC GGTGCACAGC 
CAGGACAAGT CCATCCCCCC ATGTCCGCAG ACCTGGAGGA GCCTCTGGAT CGGGTATTCA 
TTCCTGATGC ACACAGGAGC TGGGGACCAA GGAGGAGGGC AGGCCCTTAT GTCACCTGGC 
AGCTGCCTGG AAGATTTCAG AGCAGCACCA TTCCTTGAAT GCCAGGGCCG GCAGGGAACT 
TGCCACTTTT TTGCAAATAA GTATAGCTTC TGGCTCACAA CGGTGAAAGC AGACTTGCAG 
TTTTCCTCTG CTCCAGCACC AGACACCTTA AAAGAAAGCC AGGCCCAACG CCAGAAAATC 
AGCCGGTGCC AGGTCTGCGT GAAGTATAGC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009442739.2
CDS	190..5262
Translation	MWSLHIVLMRCSFRLTKSLATDPWSLTLILFSVQYVYGSGKKYVGPCGGRDCSVCHCVPEKGSRGPPGPPGPQGPIGPLGAPGPIGLSGEKGMRGDHGPPGAAGDKGDKGSTGVPGFPGLDGIPGHPGPPGPRGKPGMSGHNGSRGDPGFPGGRGALGPGGPLGHPGEKGEKGNSVFILGAVKGIQGDRGDPGLPGLPGSWGAGGPAGPTGYPGEPGLVGPPGQPGRPGLKGNPGVGVKGQMGDPGEVGQQGSPGPTLLVEPPDFRLYKGEKGIKGIPGMVGLPGPPGRKGESDIGAKGEKGIPGFPGPRGDPGSYGSPGFPGLKGELGLVGDPGLFGLIGPKGDPGNRGHPGPPGVLVTPPLPLKGPPGDPGFPGRYGETGDVGPPGPPGLLGRPGEACAGMIGPPGPQGFPGLPGLPGEAGIPGRPDSAPGKPGKPGSPGLPGAPGLQGLPGSSVIYCSVGNPGPQGIKGKVGPPGGRGSKGEKGNEGLCACEPGPMGPPGPPGLPGRQGSKGDLGLPGWLGTKGDPGPPGAEGPPGLPGKHGASGPPGNKGAKGDMVVSRVKGHKGERGPDGPPGFPGQPGSHGRDGHAGEKGDPGPPGDHEDATPGGKGFPGPLGPPGKAGPLGPPGLGFPGPPGQRGHPGVPGHPGVRGPDGLKGQKGDTISCNVTYPGRHGPPGFDGPPGPKGFPGPQGAPGLSGSDGHKGRPGTPGTAEIPGPPGFRGDMGDPGFGGEKGSSPAGPPGPPGSPGVNGQKGIPGDPAFGHLGPPGKRGLSGVPGIKGPRGDPGCPGAEGPAGIPGFPGLKGPKGREGHAGFPGVPGPPGHSCERGAPGIPGQPGLPGYPGSPGAPGGKGQLGDVGPPGPAGMKGLPGLPGWPGAHGPPGLPGIPGPFGDDGLPGPPGPKGPQGLPGFPGFPGERGKPGAEGCPGTKGEPGEKGMSGLPGDRGLRGAKGAIGPPGDEGEMAIISQKGTPGEPGPPGDDGFPGERGDKGTPGMQGRRGEPGRYGPPGFQRGEPGEKGQPGHPGPPGPPGSTGLRGFIGFPGLPGDQGEPGSPGPPGFSGIDGARGPKGNKGDPASHFGPPGPKGEPGSPGCPGHFGASGEQGLPGIQGPRGSPGRPGPPGSSGPPGCPGDHGMPGLRGQPGEMGDPGPRGLQGDPGIPGPPGIKGPSGSPGLNGLHGLKGQKGTKGASGLHDVGPPGPVGIPGLKGERGDPGSPGISPPGPRGEKGPPGPPGSSGPPGPAGATGRAPKDIPDPGPPGDQGPPGPDGPRGAPGPPGLPGSVDLLRGEPGDCGLPGPPGPPGPPGPPGYKGFPGCDGKDGQKGPMGFPGPQGPHGFPGPPGEKGLPGPPGRKGPTGLPGPRGEPGPPADVDDCPRIPGLPGAPGMRGPEGAMGLSGMRGPPGPGCKGEPGLDGRRGVDGVPGSPGPPGRKGDTGEDGYPGGPGPPGPIGDPGPKGFGPGYLGGFLLVLHSQTDQEPTCPLGMPRLWTGYSLLYLEGQEKAHNQDLGLAGSCLPVFSTLPFAYCNIHQVCHYAQRNDRSYWLASAAPLPMMPLSEEAIRPYVSRCAVCEAPAQAVAVHSQDKSIPPCPQTWRSLWIGYSFLMHTGAGDQGGGQALMSPGSCLEDFRAAPFLECQGRQGTCHFFANKYSFWLTTVKADLQFSSAPAPDTLKESQAQRQKISRCQVCVKYS

Target ORF information:

RefSeq Version	XM_009444464.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes collagen type IV alpha 4 (COL4A4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009444464.2

ORF Insert Sequence:

ATGTGGTCTC TGCACATAGT ACTAATGAGG TGCTCCTTCA GATTGACCAA GTCCTTGGCC 
ACAGATCCCT GGTCACTTAC ACTCATTCTC TTTTCTGTAC AATATGTATA TGGGAGTGGA 
AAGAAATACG TTGGTCCTTG TGGAGGAAGA GATTGCTCTG TTTGCCACTG TGTTCCTGAA 
AAGGGGTCTC GGGGTCCACC AGGACCACCA GGGCCACAGG GTCCAATTGG ACCCCTGGGA 
GCCCCAGGAC CCATTGGGCT TTCAGGAGAG AAAGGAATGA GAGGGGACCA CGGCCCTCCT 
GGAGCAGCAG GGGACAAAGG AGATAAGGGT TCAACTGGTG TTCCTGGATT TCCAGGTTTA 
GATGGCATAC CTGGGCACCC AGGGCCTCCT GGACCCAGAG GCAAACCTGG TATGAGTGGC 
CACAATGGCT CAAGAGGTGA CCCAGGGTTT CCAGGAGGAA GAGGAGCTCT TGGCCCAGGA 
GGCCCCCTAG GCCATCCTGG GGAAAAGGGA GAAAAAGGAA ATTCAGTGTT CATTTTAGGT 
GCCGTTAAAG GTATTCAGGG AGACAGAGGG GACCCAGGAC TGCCTGGCTT ACCAGGATCT 
TGGGGGGCAG GAGGACCGGC GGGTCCCACA GGATATCCTG GAGAGCCAGG GTTAGTGGGA 
CCTCCGGGCC AACCAGGGCG TCCAGGTTTG AAGGGAAATC CCGGTGTGGG AGTAAAGGGG 
CAAATGGGAG ACCCGGGTGA GGTTGGTCAG CAAGGTTCTC CTGGACCCAC CCTGTTGGTG 
GAGCCACCTG ACTTTCGTCT CTATAAAGGA GAAAAGGGTA TAAAAGGAAT TCCTGGAATG 
GTTGGACTGC CAGGACCACC AGGACGCAAG GGAGAATCTG ATATTGGGGC AAAAGGAGAA 
AAAGGTATTC CTGGATTTCC AGGGCCTCGG GGGGATCCTG GTTCCTATGG ATCTCCAGGT 
TTTCCAGGAT TAAAGGGAGA ACTAGGACTG GTTGGAGATC CTGGGCTATT TGGATTAATT 
GGCCCAAAGG GGGATCCTGG AAATCGAGGG CACCCAGGAC CACCAGGTGT TTTGGTGACT 
CCACCTCTTC CACTCAAAGG CCCACCAGGG GACCCAGGGT TCCCTGGCCG CTATGGAGAA 
ACAGGGGATG TTGGACCACC TGGTCCCCCA GGTCTCTTGG GCAGACCAGG GGAAGCCTGT 
GCAGGCATGA TAGGACCCCC TGGGCCACAA GGATTTCCTG GTCTTCCTGG GCTTCCAGGA 
GAAGCTGGTA TTCCTGGGAG ACCTGATTCT GCTCCAGGAA AACCAGGGAA GCCAGGATCA 
CCTGGCTTGC CTGGAGCACC AGGCCTGCAG GGCCTCCCAG GATCAAGTGT GATATACTGT 
AGTGTTGGGA ATCCTGGACC ACAAGGAATA AAAGGCAAAG TGGGTCCCCC AGGAGGAAGA 
GGCTCAAAAG GAGAAAAAGG AAATGAAGGA CTCTGTGCCT GTGAGCCTGG ACCCATGGGC 
CCCCCTGGCC CTCCAGGACT TCCTGGGAGG CAGGGGAGTA AGGGAGACTT GGGGCTCCCT 
GGCTGGCTTG GAACAAAAGG TGACCCAGGA CCTCCTGGTG CTGAAGGACC TCCAGGGCTA 
CCAGGAAAGC ATGGTGCCTC TGGACCACCT GGCAACAAAG GGGCGAAGGG TGACATGGTT 
GTATCAAGAG TTAAAGGGCA CAAAGGAGAA AGAGGTCCTG ATGGGCCCCC AGGATTTCCA 
GGGCAGCCAG GATCACATGG TCGGGATGGA CATGCTGGAG AAAAAGGGGA TCCAGGACCC 
CCAGGGGATC ATGAAGATGC GACCCCAGGT GGTAAAGGAT TTCCTGGACC TCTGGGCCCC 
CCGGGCAAAG CAGGACCTTT GGGGCCCCCA GGACTGGGAT TTCCTGGTCC ACCAGGACAG 
CGAGGCCACC CAGGAGTTCC AGGCCACCCA GGTGTGAGGG GCCCTGATGG CTTGAAGGGT 
CAGAAAGGTG ACACAATTTC TTGCAACGTA ACCTACCCTG GGAGGCACGG CCCTCCAGGT 
TTTGATGGAC CTCCAGGTCC GAAGGGATTT CCAGGTCCCC AAGGTGCCCC TGGGCTGAGT 
GGTTCAGATG GGCATAAAGG CAGACCTGGC ACACCAGGAA CAGCGGAAAT ACCAGGTCCA 
CCTGGTTTTC GTGGTGACAT GGGAGATCCG GGTTTTGGAG GTGAAAAGGG GTCCTCCCCT 
GCTGGGCCCC CAGGCCCTCC CGGCTCACCA GGAGTGAATG GTCAGAAAGG AATCCCGGGA 
GACCCTGCAT TTGGTCACCT GGGACCCCCG GGAAAGAGGG GTCTTTCAGG AGTGCCAGGG 
ATAAAAGGAC CCAGAGGTGA TCCGGGATGT CCAGGGGCTG AAGGGCCAGC TGGCATTCCT 
GGATTCCCAG GTCTCAAAGG TCCCAAAGGC AGAGAGGGAC ATGCTGGGTT TCCAGGTGTC 
CCAGGTCCAC CTGGCCATTC CTGTGAAAGA GGTGCTCCAG GGATACCAGG GCAACCGGGA 
CTCCCTGGGT ATCCAGGTAG CCCAGGTGCT CCAGGTGGGA AAGGACAGCT GGGAGATGTG 
GGGCCTCCCG GGCCAGCTGG AATGAAAGGC CTCCCTGGAC TCCCAGGATG GCCTGGGGCA 
CATGGTCCCC CAGGCCTCCC AGGAATCCCA GGTCCCTTTG GAGATGATGG GCTACCTGGT 
CCTCCAGGTC CAAAGGGACC CCAGGGGCTG CCTGGTTTCC CAGGTTTTCC CGGAGAAAGA 
GGAAAGCCTG GTGCAGAGGG ATGTCCTGGC ACAAAGGGAG AACCTGGAGA GAAGGGCATG 
TCTGGCCTTC CCGGAGACCG GGGACTGAGA GGGGCCAAAG GAGCCATAGG ACCTCCCGGA 
GATGAAGGAG AAATGGCTAT CATTTCCCAA AAGGGAACAC CTGGGGAACC TGGACCTCCT 
GGAGATGATG GATTCCCAGG AGAAAGAGGT GATAAAGGAA CTCCCGGGAT GCAAGGGAGA 
AGAGGAGAGC CGGGAAGATA CGGACCACCT GGATTTCAGA GAGGGGAACC TGGTGAGAAA 
GGTCAGCCAG GGCATCCTGG ACCCCCAGGC CCTCCAGGCT CAACTGGTCT AAGAGGGTTC 
ATTGGTTTTC CAGGACTTCC AGGTGACCAG GGTGAGCCAG GTTCTCCAGG TCCCCCTGGA 
TTTTCAGGAA TTGATGGAGC AAGAGGACCT AAAGGAAACA AAGGTGACCC TGCCAGTCAC 
TTTGGTCCAC CTGGTCCAAA GGGTGAGCCA GGTAGCCCTG GATGTCCAGG GCATTTTGGA 
GCATCTGGAG AGCAGGGCTT GCCTGGTATT CAAGGGCCCA GAGGATCACC TGGAAGGCCA 
GGGCCACCTG GCTCCTCTGG ACCACCAGGG TGCCCAGGTG ATCACGGGAT GCCTGGGCTG 
AGGGGACAGC CAGGAGAAAT GGGAGACCCT GGGCCAAGAG GCCTCCAGGG GGATCCAGGG 
ATACCAGGTC CTCCGGGAAT AAAAGGTCCC TCCGGATCAC CTGGCCTGAA CGGCTTGCAT 
GGATTGAAAG GTCAGAAAGG AACTAAAGGT GCTTCAGGTT TGCATGATGT GGGGCCACCT 
GGTCCAGTGG GAATACCTGG GCTAAAAGGG GAGAGAGGAG ACCCTGGGAG CCCAGGAATC 
TCTCCTCCAG GTCCTCGTGG AGAAAAAGGT CCCCCAGGAC CCCCAGGGAG TTCAGGACCA 
CCTGGTCCTG CAGGTGCCAC AGGAAGAGCT CCTAAGGACA TTCCTGACCC GGGTCCACCT 
GGAGATCAGG GACCTCCTGG TCCTGATGGC CCAAGAGGAG CACCTGGGCC TCCAGGCCTC 
CCTGGGAGTG TTGACCTTCT GAGAGGGGAG CCAGGTGACT GTGGTCTACC AGGGCCACCA 
GGTCCCCCTG GCCCACCAGG CCCTCCAGGA TACAAAGGCT TTCCAGGATG TGATGGAAAA 
GATGGCCAGA AAGGACCAAT GGGATTCCCG GGACCGCAGG GACCACATGG ATTTCCTGGG 
CCACCTGGAG AGAAGGGTTT ACCTGGACCT CCAGGGAGAA AAGGGCCCAC TGGTCTTCCA 
GGTCCCAGAG GTGAACCGGG GCCACCTGCA GATGTGGATG ACTGTCCCCG AATCCCAGGC 
CTTCCTGGGG CGCCAGGCAT GAGAGGACCA GAAGGAGCCA TGGGGCTCTC TGGAATGAGA 
GGCCCCCCAG GACCAGGGTG CAAAGGAGAG CCTGGGCTGG ATGGCAGGAG GGGTGTGGAT 
GGCGTCCCTG GGTCTCCTGG GCCTCCCGGA CGTAAAGGTG ACACAGGAGA AGACGGCTAC 
CCTGGAGGAC CAGGGCCTCC TGGTCCCATT GGGGATCCTG GGCCCAAAGG GTTTGGCCCT 
GGATACCTCG GTGGCTTCCT CCTGGTTCTC CACAGTCAGA CGGACCAGGA GCCCACCTGC 
CCCCTGGGCA TGCCCAGGCT CTGGACTGGG TATAGTCTGT TATACCTGGA AGGGCAAGAG 
AAAGCTCACA ATCAAGACCT TGGTCTGGCA GGGTCTTGCC TTCCCGTGTT TAGCACGCTG 
CCCTTTGCCT ACTGCAACAT CCACCAGGTG TGCCACTATG CCCAGAGAAA CGACAGATCC 
TACTGGCTGG CCAGCGCTGC GCCCCTCCCC ATGATGCCAC TCTCTGAAGA GGCGATCCGC 
CCCTATGTCA GCCGCTGTGC GGTATGCGAG GCCCCGGCCC AGGCGGTGGC GGTGCACAGC 
CAGGACAAGT CCATCCCCCC ATGTCCGCAG ACCTGGAGGA GCCTCTGGAT CGGGTATTCA 
TTCCTGATGC ACACAGGAGC TGGGGACCAA GGAGGAGGGC AGGCCCTTAT GTCACCTGGC 
AGCTGCCTGG AAGATTTCAG AGCAGCACCA TTCCTTGAAT GCCAGGGCCG GCAGGGAACT 
TGCCACTTTT TTGCAAATAA GTATAGCTTC TGGCTCACAA CGGTGAAAGC AGACTTGCAG 
TTTTCCTCTG CTCCAGCACC AGACACCTTA AAAGAAAGCC AGGCCCAACG CCAGAAAATC 
AGCCGGTGCC AGGTCTGCGT GAAGTATAGC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL4A4 ( XM_009444466.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_009442741.2 Pan troglodytes collagen type IV alpha 4 (COL4A4), transcript variant X5, mRNA.

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CloneID	OPc80764
Clone ID Related Accession (Same CDS sequence)	XM_009444466.2
Accession Version	XM_009444466.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5028bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	collagen alpha-4(IV) chain isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006470.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 2, 2016 this sequence version replaced XM_009444466.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGTCTC TGCACATAGT ACTAATGAGG TGCTCCTTCA GATTGACCAA GTCCTTGGCC 
ACAGATCCCT GGTCACTTAC ACTCATTCTC TTTTCTGTAC AATATGTATA TGGGAGTGGA 
AAGAAATACG TTGGTCCTTG TGGAGGAAGA GATTGCTCTG TTTGCCACTG TGTTCCTGAA 
AAGGGGTCTC GGGGTCCACC AGGACCACCA GGGCCACAGG GTCCAATTGG ACCCCTGGGA 
GCCCCAGGAC CCATTGGGCT TTCAGGAGAG AAAGGAATGA GAGGGGACCA CGGCCCTCCT 
GGAGCAGCAG GGGACAAAGG AGATAAGGGT TCAACTGGTG TTCCTGGATT TCCAGGTTTA 
GATGGCATAC CTGGGCACCC AGGGCCTCCT GGACCCAGAG GCAAACCTGG TATGAGTGGC 
CACAATGGCT CAAGAGGTGA CCCAGGGTTT CCAGGAGGAA GAGGAGCTCT TGGCCCAGGA 
GGCCCCCTAG GCCATCCTGG GGAAAAGGGA GAAAAAGGAA ATTCAGTGTT CATTTTAGGT 
GCCGTTAAAG GTATTCAGGG AGACAGAGGG GACCCAGGAC TGCCTGGCTT ACCAGGATCT 
TGGGGGGCAG GAGGACCGGC GGGTCCCACA GGATATCCTG GAGAGCCAGG GTTAGTGGGA 
CCTCCGGGCC AACCAGGGCG TCCAGGTTTG AAGGGAAATC CCGGTGTGGG AGTAAAGGGG 
CAAATGGGAG ACCCGGGTGA GGTTGGTCAG CAAGGTTCTC CTGGACCCAC CCTGTTGGTG 
GAGCCACCTG ACTTTCGTCT CTATAAAGGA GAAAAGGGTA TAAAAGGAAT TCCTGGAATG 
GTTGGACTGC CAGGACCACC AGGACGCAAG GGAGAATCTG ATATTGGGGC AAAAGGAGAA 
AAAGGTATTC CTGGATTTCC AGGGCCTCGG GGAGAACTAG GACTGGTTGG AGATCCTGGG 
CTATTTGGAT TAATTGGCCC AAAGGGGGAT CCTGGAAATC GAGGGCACCC AGGACCACCA 
GGTGTTTTGG TGACTCCACC TCTTCCACTC AAAGGCCCAC CAGGGGACCC AGGGTTCCCT 
GGCCGCTATG GAGAAACAGG GGATGTTGGA CCACCTGGTC CCCCAGGTCT CTTGGGCAGA 
CCAGGGGAAG CCTGTGCAGG CATGATAGGA CCCCCTGGGC CACAAGGATT TCCTGGTCTT 
CCTGGGCTTC CAGGAGAAGC TGGTATTCCT GGGAGACCTG ATTCTGCTCC AGGAAAACCA 
GGGAAGCCAG GATCACCTGG CTTGCCTGGA GCACCAGGCC TGCAGGGCCT CCCAGGATCA 
AGTGTGATAT ACTGTAGTGT TGGGAATCCT GGACCACAAG GAATAAAAGG CAAAGTGGGT 
CCCCCAGGAG GAAGAGGCTC AAAAGGAGAA AAAGGAAATG AAGGACTCTG TGCCTGTGAG 
CCTGGACCCA TGGGCCCCCC TGGCCCTCCA GGACTTCCTG GGAGGCAGGG GAGTAAGGGA 
GACTTGGGGC TCCCTGGCTG GCTTGGAACA AAAGGTGACC CAGGACCTCC TGGTGCTGAA 
GGACCTCCAG GGCTACCAGG AAAGCATGGT GCCTCTGGAC CACCTGGCAA CAAAGGGGCG 
AAGGGTGACA TGGTTGTATC AAGAGTTAAA GGGCACAAAG GAGAAAGAGG TCCTGATGGG 
CCCCCAGGAT TTCCAGGGCA GCCAGGATCA CATGGTCGGG ATGGACATGC TGGAGAAAAA 
GGGGATCCAG GACCCCCAGG GGATCATGAA GATGCGACCC CAGGTGGTAA AGGATTTCCT 
GGACCTCTGG GCCCCCCGGG CAAAGCAGGA CCTTTGGGGC CCCCAGGACT GGGATTTCCT 
GGTCCACCAG GACAGCGAGG CCACCCAGGA GTTCCAGGCC ACCCAGGTGT GAGGGGCCCT 
GATGGCTTGA AGGGTCAGAA AGGTGACACA ATTTCTTGCA ACGTAACCTA CCCTGGGAGG 
CACGGCCCTC CAGGTTTTGA TGGACCTCCA GGTCCGAAGG GATTTCCAGG TCCCCAAGGT 
GCCCCTGGGC TGAGTGGTTC AGATGGGCAT AAAGGCAGAC CTGGCACACC AGGAACAGCG 
GAAATACCAG GTCCACCTGG TTTTCGTGGT GACATGGGAG ATCCGGGTTT TGGAGGTGAA 
AAGGGGTCCT CCCCTGCTGG GCCCCCAGGC CCTCCCGGCT CACCAGGAGT GAATGGTCAG 
AAAGGAATCC CGGGAGACCC TGCATTTGGT CACCTGGGAC CCCCGGGAAA GAGGGGTCTT 
TCAGGAGTGC CAGGGATAAA AGGACCCAGA GGTGATCCGG GATGTCCAGG GGCTGAAGGG 
CCAGCTGGCA TTCCTGGATT CCCAGGTCTC AAAGGTCCCA AAGGCAGAGA GGGACATGCT 
GGGTTTCCAG GTGTCCCAGG TCCACCTGGC CATTCCTGTG AAAGAGGTGC TCCAGGGATA 
CCAGGGCAAC CGGGACTCCC TGGGTATCCA GGTAGCCCAG GTGCTCCAGG TGGGAAAGGA 
CAGCTGGGAG ATGTGGGGCC TCCCGGGCCA GCTGGAATGA AAGGCCTCCC TGGACTCCCA 
GGATGGCCTG GGGCACATGG TCCCCCAGGC CTCCCAGGAA TCCCAGGTCC CTTTGGAGAT 
GATGGGCTAC CTGGTCCTCC AGGTCCAAAG GGACCCCAGG GGCTGCCTGG TTTCCCAGGT 
TTTCCCGGAG AAAGAGGAAA GCCTGGTGCA GAGGGATGTC CTGGCACAAA GGGAGAACCT 
GGAGAGAAGG GCATGTCTGG CCTTCCCGGA GACCGGGGAC TGAGAGGGGC CAAAGGAGCC 
ATAGGACCTC CCGGAGATGA AGGAGAAATG GCTATCATTT CCCAAAAGGG AACACCTGGG 
GAACCTGGAC CTCCTGGAGA TGATGGATTC CCAGGAGAAA GAGGTGATAA AGGAACTCCC 
GGGATGCAAG GGAGAAGAGG AGAGCCGGGA AGATACGGAC CACCTGGATT TCAGAGAGGG 
GAACCTGGTG AGAAAGGTCA GCCAGGGCAT CCTGGACCCC CAGGCCCTCC AGGCTCAACT 
GGTCTAAGAG GGTTCATTGG TTTTCCAGGA CTTCCAGGTG ACCAGGGTGA GCCAGGTTCT 
CCAGGTCCCC CTGGATTTTC AGGAATTGAT GGAGCAAGAG GACCTAAAGG AAACAAAGGT 
GACCCTGCCA GTCACTTTGG TCCACCTGGT CCAAAGGGTG AGCCAGGTAG CCCTGGATGT 
CCAGGGCATT TTGGAGCATC TGGAGAGCAG GGCTTGCCTG GTATTCAAGG GCCCAGAGGA 
TCACCTGGAA GGCCAGGGCC ACCTGGCTCC TCTGGACCAC CAGGGTGCCC AGGTGATCAC 
GGGATGCCTG GGCTGAGGGG ACAGCCAGGA GAAATGGGAG ACCCTGGGCC AAGAGGCCTC 
CAGGGGGATC CAGGGATACC AGGTCCTCCG GGAATAAAAG GTCCCTCCGG ATCACCTGGC 
CTGAACGGCT TGCATGGATT GAAAGGTCAG AAAGGAACTA AAGGTGCTTC AGGTTTGCAT 
GATGTGGGGC CACCTGGTCC AGTGGGAATA CCTGGGCTAA AAGGGGAGAG AGGAGACCCT 
GGGAGCCCAG GAATCTCTCC TCCAGGTCCT CGTGGAGAAA AAGGTCCCCC AGGACCCCCA 
GGGAGTTCAG GACCACCTGG TCCTGCAGGT GCCACAGGAA GAGCTCCTAA GGACATTCCT 
GACCCGGGTC CACCTGGAGA TCAGGGACCT CCTGGTCCTG ATGGCCCAAG AGGAGCACCT 
GGGCCTCCAG GCCTCCCTGG GAGTGTTGAC CTTCTGAGAG GGGAGCCAGG TGACTGTGGT 
CTACCAGGGC CACCAGGTCC CCCTGGCCCA CCAGGCCCTC CAGGATACAA AGGCTTTCCA 
GGATGTGATG GAAAAGATGG CCAGAAAGGA CCAATGGGAT TCCCGGGACC GCAGGGACCA 
CATGGATTTC CTGGGCCACC TGGAGAGAAG GGTTTACCTG GACCTCCAGG GAGAAAAGGG 
CCCACTGGTC TTCCAGGTCC CAGAGGTGAA CCGGGGCCAC CTGCAGATGT GGATGACTGT 
CCCCGAATCC CAGGCCTTCC TGGGGCGCCA GGCATGAGAG GACCAGAAGG AGCCATGGGG 
CTCTCTGGAA TGAGAGGCCC CCCAGGACCA GGGTGCAAAG GAGAGCCTGG GCTGGATGGC 
AGGAGGGGTG TGGATGGCGT CCCTGGGTCT CCTGGGCCTC CCGGACGTAA AGGTGACACA 
GGAGAAGACG GCTACCCTGG AGGACCAGGG CCTCCTGGTC CCATTGGGGA TCCTGGGCCC 
AAAGGGTTTG GCCCTGGATA CCTCGGTGGC TTCCTCCTGG TTCTCCACAG TCAGACGGAC 
CAGGAGCCCA CCTGCCCCCT GGGCATGCCC AGGCTCTGGA CTGGGTATAG TCTGTTATAC 
CTGGAAGGGC AAGAGAAAGC TCACAATCAA GACCTTGGTC TGGCAGGGTC TTGCCTTCCC 
GTGTTTAGCA CGCTGCCCTT TGCCTACTGC AACATCCACC AGGTGTGCCA CTATGCCCAG 
AGAAACGACA GATCCTACTG GCTGGCCAGC GCTGCGCCCC TCCCCATGAT GCCACTCTCT 
GAAGAGGCGA TCCGCCCCTA TGTCAGCCGC TGTGCGGTAT GCGAGGCCCC GGCCCAGGCG 
GTGGCGGTGC ACAGCCAGGA CAAGTCCATC CCCCCATGTC CGCAGACCTG GAGGAGCCTC 
TGGATCGGGT ATTCATTCCT GATGCACACA GGAGCTGGGG ACCAAGGAGG AGGGCAGGCC 
CTTATGTCAC CTGGCAGCTG CCTGGAAGAT TTCAGAGCAG CACCATTCCT TGAATGCCAG 
GGCCGGCAGG GAACTTGCCA CTTTTTTGCA AATAAGTATA GCTTCTGGCT CACAACGGTG 
AAAGCAGACT TGCAGTTTTC CTCTGCTCCA GCACCAGACA CCTTAAAAGA AAGCCAGGCC 
CAACGCCAGA AAATCAGCCG GTGCCAGGTC TGCGTGAAGT ATAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009442741.2
CDS	425..5452
Translation	MWSLHIVLMRCSFRLTKSLATDPWSLTLILFSVQYVYGSGKKYVGPCGGRDCSVCHCVPEKGSRGPPGPPGPQGPIGPLGAPGPIGLSGEKGMRGDHGPPGAAGDKGDKGSTGVPGFPGLDGIPGHPGPPGPRGKPGMSGHNGSRGDPGFPGGRGALGPGGPLGHPGEKGEKGNSVFILGAVKGIQGDRGDPGLPGLPGSWGAGGPAGPTGYPGEPGLVGPPGQPGRPGLKGNPGVGVKGQMGDPGEVGQQGSPGPTLLVEPPDFRLYKGEKGIKGIPGMVGLPGPPGRKGESDIGAKGEKGIPGFPGPRGELGLVGDPGLFGLIGPKGDPGNRGHPGPPGVLVTPPLPLKGPPGDPGFPGRYGETGDVGPPGPPGLLGRPGEACAGMIGPPGPQGFPGLPGLPGEAGIPGRPDSAPGKPGKPGSPGLPGAPGLQGLPGSSVIYCSVGNPGPQGIKGKVGPPGGRGSKGEKGNEGLCACEPGPMGPPGPPGLPGRQGSKGDLGLPGWLGTKGDPGPPGAEGPPGLPGKHGASGPPGNKGAKGDMVVSRVKGHKGERGPDGPPGFPGQPGSHGRDGHAGEKGDPGPPGDHEDATPGGKGFPGPLGPPGKAGPLGPPGLGFPGPPGQRGHPGVPGHPGVRGPDGLKGQKGDTISCNVTYPGRHGPPGFDGPPGPKGFPGPQGAPGLSGSDGHKGRPGTPGTAEIPGPPGFRGDMGDPGFGGEKGSSPAGPPGPPGSPGVNGQKGIPGDPAFGHLGPPGKRGLSGVPGIKGPRGDPGCPGAEGPAGIPGFPGLKGPKGREGHAGFPGVPGPPGHSCERGAPGIPGQPGLPGYPGSPGAPGGKGQLGDVGPPGPAGMKGLPGLPGWPGAHGPPGLPGIPGPFGDDGLPGPPGPKGPQGLPGFPGFPGERGKPGAEGCPGTKGEPGEKGMSGLPGDRGLRGAKGAIGPPGDEGEMAIISQKGTPGEPGPPGDDGFPGERGDKGTPGMQGRRGEPGRYGPPGFQRGEPGEKGQPGHPGPPGPPGSTGLRGFIGFPGLPGDQGEPGSPGPPGFSGIDGARGPKGNKGDPASHFGPPGPKGEPGSPGCPGHFGASGEQGLPGIQGPRGSPGRPGPPGSSGPPGCPGDHGMPGLRGQPGEMGDPGPRGLQGDPGIPGPPGIKGPSGSPGLNGLHGLKGQKGTKGASGLHDVGPPGPVGIPGLKGERGDPGSPGISPPGPRGEKGPPGPPGSSGPPGPAGATGRAPKDIPDPGPPGDQGPPGPDGPRGAPGPPGLPGSVDLLRGEPGDCGLPGPPGPPGPPGPPGYKGFPGCDGKDGQKGPMGFPGPQGPHGFPGPPGEKGLPGPPGRKGPTGLPGPRGEPGPPADVDDCPRIPGLPGAPGMRGPEGAMGLSGMRGPPGPGCKGEPGLDGRRGVDGVPGSPGPPGRKGDTGEDGYPGGPGPPGPIGDPGPKGFGPGYLGGFLLVLHSQTDQEPTCPLGMPRLWTGYSLLYLEGQEKAHNQDLGLAGSCLPVFSTLPFAYCNIHQVCHYAQRNDRSYWLASAAPLPMMPLSEEAIRPYVSRCAVCEAPAQAVAVHSQDKSIPPCPQTWRSLWIGYSFLMHTGAGDQGGGQALMSPGSCLEDFRAAPFLECQGRQGTCHFFANKYSFWLTTVKADLQFSSAPAPDTLKESQAQRQKISRCQVCVKYS

Target ORF information:

RefSeq Version	XM_009444466.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes collagen type IV alpha 4 (COL4A4), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009444466.2

ORF Insert Sequence:

ATGTGGTCTC TGCACATAGT ACTAATGAGG TGCTCCTTCA GATTGACCAA GTCCTTGGCC 
ACAGATCCCT GGTCACTTAC ACTCATTCTC TTTTCTGTAC AATATGTATA TGGGAGTGGA 
AAGAAATACG TTGGTCCTTG TGGAGGAAGA GATTGCTCTG TTTGCCACTG TGTTCCTGAA 
AAGGGGTCTC GGGGTCCACC AGGACCACCA GGGCCACAGG GTCCAATTGG ACCCCTGGGA 
GCCCCAGGAC CCATTGGGCT TTCAGGAGAG AAAGGAATGA GAGGGGACCA CGGCCCTCCT 
GGAGCAGCAG GGGACAAAGG AGATAAGGGT TCAACTGGTG TTCCTGGATT TCCAGGTTTA 
GATGGCATAC CTGGGCACCC AGGGCCTCCT GGACCCAGAG GCAAACCTGG TATGAGTGGC 
CACAATGGCT CAAGAGGTGA CCCAGGGTTT CCAGGAGGAA GAGGAGCTCT TGGCCCAGGA 
GGCCCCCTAG GCCATCCTGG GGAAAAGGGA GAAAAAGGAA ATTCAGTGTT CATTTTAGGT 
GCCGTTAAAG GTATTCAGGG AGACAGAGGG GACCCAGGAC TGCCTGGCTT ACCAGGATCT 
TGGGGGGCAG GAGGACCGGC GGGTCCCACA GGATATCCTG GAGAGCCAGG GTTAGTGGGA 
CCTCCGGGCC AACCAGGGCG TCCAGGTTTG AAGGGAAATC CCGGTGTGGG AGTAAAGGGG 
CAAATGGGAG ACCCGGGTGA GGTTGGTCAG CAAGGTTCTC CTGGACCCAC CCTGTTGGTG 
GAGCCACCTG ACTTTCGTCT CTATAAAGGA GAAAAGGGTA TAAAAGGAAT TCCTGGAATG 
GTTGGACTGC CAGGACCACC AGGACGCAAG GGAGAATCTG ATATTGGGGC AAAAGGAGAA 
AAAGGTATTC CTGGATTTCC AGGGCCTCGG GGAGAACTAG GACTGGTTGG AGATCCTGGG 
CTATTTGGAT TAATTGGCCC AAAGGGGGAT CCTGGAAATC GAGGGCACCC AGGACCACCA 
GGTGTTTTGG TGACTCCACC TCTTCCACTC AAAGGCCCAC CAGGGGACCC AGGGTTCCCT 
GGCCGCTATG GAGAAACAGG GGATGTTGGA CCACCTGGTC CCCCAGGTCT CTTGGGCAGA 
CCAGGGGAAG CCTGTGCAGG CATGATAGGA CCCCCTGGGC CACAAGGATT TCCTGGTCTT 
CCTGGGCTTC CAGGAGAAGC TGGTATTCCT GGGAGACCTG ATTCTGCTCC AGGAAAACCA 
GGGAAGCCAG GATCACCTGG CTTGCCTGGA GCACCAGGCC TGCAGGGCCT CCCAGGATCA 
AGTGTGATAT ACTGTAGTGT TGGGAATCCT GGACCACAAG GAATAAAAGG CAAAGTGGGT 
CCCCCAGGAG GAAGAGGCTC AAAAGGAGAA AAAGGAAATG AAGGACTCTG TGCCTGTGAG 
CCTGGACCCA TGGGCCCCCC TGGCCCTCCA GGACTTCCTG GGAGGCAGGG GAGTAAGGGA 
GACTTGGGGC TCCCTGGCTG GCTTGGAACA AAAGGTGACC CAGGACCTCC TGGTGCTGAA 
GGACCTCCAG GGCTACCAGG AAAGCATGGT GCCTCTGGAC CACCTGGCAA CAAAGGGGCG 
AAGGGTGACA TGGTTGTATC AAGAGTTAAA GGGCACAAAG GAGAAAGAGG TCCTGATGGG 
CCCCCAGGAT TTCCAGGGCA GCCAGGATCA CATGGTCGGG ATGGACATGC TGGAGAAAAA 
GGGGATCCAG GACCCCCAGG GGATCATGAA GATGCGACCC CAGGTGGTAA AGGATTTCCT 
GGACCTCTGG GCCCCCCGGG CAAAGCAGGA CCTTTGGGGC CCCCAGGACT GGGATTTCCT 
GGTCCACCAG GACAGCGAGG CCACCCAGGA GTTCCAGGCC ACCCAGGTGT GAGGGGCCCT 
GATGGCTTGA AGGGTCAGAA AGGTGACACA ATTTCTTGCA ACGTAACCTA CCCTGGGAGG 
CACGGCCCTC CAGGTTTTGA TGGACCTCCA GGTCCGAAGG GATTTCCAGG TCCCCAAGGT 
GCCCCTGGGC TGAGTGGTTC AGATGGGCAT AAAGGCAGAC CTGGCACACC AGGAACAGCG 
GAAATACCAG GTCCACCTGG TTTTCGTGGT GACATGGGAG ATCCGGGTTT TGGAGGTGAA 
AAGGGGTCCT CCCCTGCTGG GCCCCCAGGC CCTCCCGGCT CACCAGGAGT GAATGGTCAG 
AAAGGAATCC CGGGAGACCC TGCATTTGGT CACCTGGGAC CCCCGGGAAA GAGGGGTCTT 
TCAGGAGTGC CAGGGATAAA AGGACCCAGA GGTGATCCGG GATGTCCAGG GGCTGAAGGG 
CCAGCTGGCA TTCCTGGATT CCCAGGTCTC AAAGGTCCCA AAGGCAGAGA GGGACATGCT 
GGGTTTCCAG GTGTCCCAGG TCCACCTGGC CATTCCTGTG AAAGAGGTGC TCCAGGGATA 
CCAGGGCAAC CGGGACTCCC TGGGTATCCA GGTAGCCCAG GTGCTCCAGG TGGGAAAGGA 
CAGCTGGGAG ATGTGGGGCC TCCCGGGCCA GCTGGAATGA AAGGCCTCCC TGGACTCCCA 
GGATGGCCTG GGGCACATGG TCCCCCAGGC CTCCCAGGAA TCCCAGGTCC CTTTGGAGAT 
GATGGGCTAC CTGGTCCTCC AGGTCCAAAG GGACCCCAGG GGCTGCCTGG TTTCCCAGGT 
TTTCCCGGAG AAAGAGGAAA GCCTGGTGCA GAGGGATGTC CTGGCACAAA GGGAGAACCT 
GGAGAGAAGG GCATGTCTGG CCTTCCCGGA GACCGGGGAC TGAGAGGGGC CAAAGGAGCC 
ATAGGACCTC CCGGAGATGA AGGAGAAATG GCTATCATTT CCCAAAAGGG AACACCTGGG 
GAACCTGGAC CTCCTGGAGA TGATGGATTC CCAGGAGAAA GAGGTGATAA AGGAACTCCC 
GGGATGCAAG GGAGAAGAGG AGAGCCGGGA AGATACGGAC CACCTGGATT TCAGAGAGGG 
GAACCTGGTG AGAAAGGTCA GCCAGGGCAT CCTGGACCCC CAGGCCCTCC AGGCTCAACT 
GGTCTAAGAG GGTTCATTGG TTTTCCAGGA CTTCCAGGTG ACCAGGGTGA GCCAGGTTCT 
CCAGGTCCCC CTGGATTTTC AGGAATTGAT GGAGCAAGAG GACCTAAAGG AAACAAAGGT 
GACCCTGCCA GTCACTTTGG TCCACCTGGT CCAAAGGGTG AGCCAGGTAG CCCTGGATGT 
CCAGGGCATT TTGGAGCATC TGGAGAGCAG GGCTTGCCTG GTATTCAAGG GCCCAGAGGA 
TCACCTGGAA GGCCAGGGCC ACCTGGCTCC TCTGGACCAC CAGGGTGCCC AGGTGATCAC 
GGGATGCCTG GGCTGAGGGG ACAGCCAGGA GAAATGGGAG ACCCTGGGCC AAGAGGCCTC 
CAGGGGGATC CAGGGATACC AGGTCCTCCG GGAATAAAAG GTCCCTCCGG ATCACCTGGC 
CTGAACGGCT TGCATGGATT GAAAGGTCAG AAAGGAACTA AAGGTGCTTC AGGTTTGCAT 
GATGTGGGGC CACCTGGTCC AGTGGGAATA CCTGGGCTAA AAGGGGAGAG AGGAGACCCT 
GGGAGCCCAG GAATCTCTCC TCCAGGTCCT CGTGGAGAAA AAGGTCCCCC AGGACCCCCA 
GGGAGTTCAG GACCACCTGG TCCTGCAGGT GCCACAGGAA GAGCTCCTAA GGACATTCCT 
GACCCGGGTC CACCTGGAGA TCAGGGACCT CCTGGTCCTG ATGGCCCAAG AGGAGCACCT 
GGGCCTCCAG GCCTCCCTGG GAGTGTTGAC CTTCTGAGAG GGGAGCCAGG TGACTGTGGT 
CTACCAGGGC CACCAGGTCC CCCTGGCCCA CCAGGCCCTC CAGGATACAA AGGCTTTCCA 
GGATGTGATG GAAAAGATGG CCAGAAAGGA CCAATGGGAT TCCCGGGACC GCAGGGACCA 
CATGGATTTC CTGGGCCACC TGGAGAGAAG GGTTTACCTG GACCTCCAGG GAGAAAAGGG 
CCCACTGGTC TTCCAGGTCC CAGAGGTGAA CCGGGGCCAC CTGCAGATGT GGATGACTGT 
CCCCGAATCC CAGGCCTTCC TGGGGCGCCA GGCATGAGAG GACCAGAAGG AGCCATGGGG 
CTCTCTGGAA TGAGAGGCCC CCCAGGACCA GGGTGCAAAG GAGAGCCTGG GCTGGATGGC 
AGGAGGGGTG TGGATGGCGT CCCTGGGTCT CCTGGGCCTC CCGGACGTAA AGGTGACACA 
GGAGAAGACG GCTACCCTGG AGGACCAGGG CCTCCTGGTC CCATTGGGGA TCCTGGGCCC 
AAAGGGTTTG GCCCTGGATA CCTCGGTGGC TTCCTCCTGG TTCTCCACAG TCAGACGGAC 
CAGGAGCCCA CCTGCCCCCT GGGCATGCCC AGGCTCTGGA CTGGGTATAG TCTGTTATAC 
CTGGAAGGGC AAGAGAAAGC TCACAATCAA GACCTTGGTC TGGCAGGGTC TTGCCTTCCC 
GTGTTTAGCA CGCTGCCCTT TGCCTACTGC AACATCCACC AGGTGTGCCA CTATGCCCAG 
AGAAACGACA GATCCTACTG GCTGGCCAGC GCTGCGCCCC TCCCCATGAT GCCACTCTCT 
GAAGAGGCGA TCCGCCCCTA TGTCAGCCGC TGTGCGGTAT GCGAGGCCCC GGCCCAGGCG 
GTGGCGGTGC ACAGCCAGGA CAAGTCCATC CCCCCATGTC CGCAGACCTG GAGGAGCCTC 
TGGATCGGGT ATTCATTCCT GATGCACACA GGAGCTGGGG ACCAAGGAGG AGGGCAGGCC 
CTTATGTCAC CTGGCAGCTG CCTGGAAGAT TTCAGAGCAG CACCATTCCT TGAATGCCAG 
GGCCGGCAGG GAACTTGCCA CTTTTTTGCA AATAAGTATA GCTTCTGGCT CACAACGGTG 
AAAGCAGACT TGCAGTTTTC CTCTGCTCCA GCACCAGACA CCTTAAAAGA AAGCCAGGCC 
CAACGCCAGA AAATCAGCCG GTGCCAGGTC TGCGTGAAGT ATAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL4A4 ( XM_009444467.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_009442742.2 Pan troglodytes collagen type IV alpha 4 (COL4A4), transcript variant X6, mRNA.

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CloneID	OPc80765
Clone ID Related Accession (Same CDS sequence)	XM_009444467.2
Accession Version	XM_009444467.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4992bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	collagen alpha-4(IV) chain isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006470.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 2, 2016 this sequence version replaced XM_009444467.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGTCTC TGCACATAGT ACTAATGAGG TGCTCCTTCA GATTGACCAA GTCCTTGGCC 
ACAGATCCCT GGTCACTTAC ACTCATTCTC TTTTCTGTAC AATATGTATA TGGGAGTGGA 
AAGAAATACG TTGGTCCTTG TGGAGGAAGA GATTGCTCTG TTTGCCACTG TGTTCCTGAA 
AAGGGGTCTC GGGGTCCACC AGGACCACCA GGGCCACAGG GTCCAATTGG ACCCCTGGGA 
GCCCCAGGAC CCATTGGGCT TTCAGGAGAG AAAGGAATGA GAGGGGACCA CGGCCCTCCT 
GGAGCAGCAG GGGACAAAGG AGATAAGGGT TCAACTGGTG TTCCTGGATT TCCAGGTTTA 
GATGGCATAC CTGGGCACCC AGGGCCTCCT GGACCCAGAG GCAAACCTGG TATGAGTGGC 
CACAATGGCT CAAGAGGTGA CCCAGGGTTT CCAGGAGGAA GAGGAGCTCT TGGCCCAGGA 
GGCCCCCTAG GCCATCCTGG GGAAAAGGGA GAAAAAGGAA ATTCAGTGTT CATTTTAGGT 
GCCGTTAAAG GTATTCAGGG AGACAGAGGG GACCCAGGAC TGCCTGGCTT ACCAGGATCT 
TGGGGGGCAG GAGGACCGGC GGGTCCCACA GGATATCCTG GAGAGCCAGG GTTAGTGGGA 
CCTCCGGGCC AACCAGGGCG TCCAGGTTTG AAGGGAAATC CCGGTGTGGG AGTAAAGGGG 
CAAATGGGAG ACCCGGGTGA GGTTGGTCAG CAAGGTTCTC CTGGACCCAC CCTGTTGGTG 
GAGCCACCTG ACTTTCGTCT CTATAAAGGA GAAAAGGGTA TAAAAGGAAT TCCTGGAATG 
GTTGGACTGC CAGGACCACC AGGACGCAAG GGAGAATCTG ATATTGGGGC AAAAGGAGAA 
AAAGGTATTC CTGGATTTCC AGGGCCTCGG GGGGATCCTG GTTCCTATGG ATCTCCAGGT 
TTTCCAGGAT TAAAGGGAGA ACTAGGACTG GTTGGAGATC CTGGGCTATT TGGATTAATT 
GGCCCAAAGG GGGATCCTGG AAATCGAGGG CACCCAGGAC CACCAGGTGT TTTGGTGACT 
CCACCTCTTC CACTCAAAGG CCCACCAGGG GACCCAGGGT TCCCTGGCCG CTATGGAGAA 
ACAGGGGATG TTGGACCACC TGGTCCCCCA GGTCTCTTGG GCAGACCAGG GGAAGCCTGT 
GCAGGCATGA TAGGACCCCC TGGGCCACAA GGATTTCCTG GTCTTCCTGG GCTTCCAGGA 
GAAGCTGGTA TTCCTGGGAG ACCTGATTCT GCTCCAGGAA AACCAGGGAA GCCAGGATCA 
CCTGGCTTGC CTGGAGCACC AGGCCTGCAG GGCCTCCCAG GATCAAGTGT GATATACTGT 
AGTGTTGGGA ATCCTGGACC ACAAGGAATA AAAGGCAAAG TGGGTCCCCC AGGAGGAAGA 
GGCTCAAAAG GAGAAAAAGG AAATGAAGGA CTCTGTGCCT GTGAGCCTGG ACCCATGGGC 
CCCCCTGGCC CTCCAGGACT TCCTGGGAGG CAGGGGAGTA AGGGAGACTT GGGGCTCCCT 
GGCTGGCTTG GAACAAAAGG TGACCCAGGA CCTCCTGGTG CTGAAGGACC TCCAGGGCTA 
CCAGGAAAGC ATGGTGCCTC TGGACCACCT GGCAACAAAG GGGCGAAGGG TGACATGGTT 
GTATCAAGAG TTAAAGGGCA CAAAGGAGAA AGAGGTCCTG ATGGGCCCCC AGGATTTCCA 
GGGCAGCCAG GATCACATGG TCGGGATGGA CATGCTGGAG AAAAAGGGGA TCCAGGACCC 
CCAGGGGATC ATGAAGATGC GACCCCAGGT GGTAAAGGAT TTCCTGGACC TCTGGGCCCC 
CCGGGCAAAG CAGGACCTTT GGGGCCCCCA GGACTGGGAT TTCCTGGTCC ACCAGGACAG 
CGAGGCCACC CAGGAGTTCC AGGCCACCCA GGTGTGAGGG GCCCTGATGG CTTGAAGGGT 
CAGAAAGGTG ACACAATTTC TTGCAACGTA ACCTACCCTG GGAGGCACGG CCCTCCAGGT 
TTTGATGGAC CTCCAGGTCC GAAGGGATTT CCAGGTCCCC AAGGTGCCCC TGGGCTGAGT 
GGTTCAGATG GGCATAAAGG CAGACCTGGC ACACCAGGAA CAGCGGAAAT ACCAGGTCCA 
CCTGGTTTTC GTGGTGACAT GGGAGATCCG GGTTTTGGAG GTGAAAAGGG GTCCTCCCCT 
GCTGGGCCCC CAGGCCCTCC CGGCTCACCA GGAGTGAATG GTCAGAAAGG AATCCCGGGA 
GACCCTGCAT TTGGTCACCT GGGACCCCCG GGAAAGAGGG GTCTTTCAGG AGTGCCAGGG 
ATAAAAGGAC CCAGAGGTGA TCCGGGATGT CCAGGGGCTG AAGGGCCAGC TGGCATTCCT 
GGATTCCCAG GTCTCAAAGG TCCCAAAGGC AGAGAGGGAC ATGCTGGGTT TCCAGGTGTC 
CCAGGTCCAC CTGGCCATTC CTGTGAAAGA GGTGCTCCAG GGATACCAGG GCAACCGGGA 
CTCCCTGGGT ATCCAGGTAG CCCAGGTGCT CCAGGTGGGA AAGGACAGCT GGGAGATGTG 
GGGCCTCCCG GGCCAGCTGG AATGAAAGGC CTCCCTGGAC TCCCAGGATG GCCTGGGGCA 
CATGGTCCCC CAGGCCTCCC AGGAATCCCA GGTCCCTTTG GAGATGATGG GCTACCTGGT 
CCTCCAGGTC CAAAGGGACC CCAGGGGCTG CCTGGTTTCC CAGGTTTTCC CGGAGAAAGA 
GGAAAGCCTG GTGCAGAGGG ATGTCCTGGC ACAAAGGGAG AACCTGGAGA GAAGGGCATG 
TCTGGCCTTC CCGGAGACCG GGGACTGAGA GGGGCCAAAG GAGCCATAGG ACCTCCCGGA 
GATGAAGGAG AAATGGCTAT CATTTCCCAA AAGGGAACAC CTGGGGAACC TGGACCTCCT 
GGAGATGATG GATTCCCAGG AGAAAGAGGT GATAAAGGAA CTCCCGGGAT GCAAGGGAGA 
AGAGGAGAGC CGGGAAGATA CGGACCACCT GGATTTCAGA GAGGGGAACC TGGACTTCCA 
GGTGACCAGG GTGAGCCAGG TTCTCCAGGT CCCCCTGGAT TTTCAGGAAT TGATGGAGCA 
AGAGGACCTA AAGGAAACAA AGGTGACCCT GCCAGTCACT TTGGTCCACC TGGTCCAAAG 
GGTGAGCCAG GTAGCCCTGG ATGTCCAGGG CATTTTGGAG CATCTGGAGA GCAGGGCTTG 
CCTGGTATTC AAGGGCCCAG AGGATCACCT GGAAGGCCAG GGCCACCTGG CTCCTCTGGA 
CCACCAGGGT GCCCAGGTGA TCACGGGATG CCTGGGCTGA GGGGACAGCC AGGAGAAATG 
GGAGACCCTG GGCCAAGAGG CCTCCAGGGG GATCCAGGGA TACCAGGTCC TCCGGGAATA 
AAAGGTCCCT CCGGATCACC TGGCCTGAAC GGCTTGCATG GATTGAAAGG TCAGAAAGGA 
ACTAAAGGTG CTTCAGGTTT GCATGATGTG GGGCCACCTG GTCCAGTGGG AATACCTGGG 
CTAAAAGGGG AGAGAGGAGA CCCTGGGAGC CCAGGAATCT CTCCTCCAGG TCCTCGTGGA 
GAAAAAGGTC CCCCAGGACC CCCAGGGAGT TCAGGACCAC CTGGTCCTGC AGGTGCCACA 
GGAAGAGCTC CTAAGGACAT TCCTGACCCG GGTCCACCTG GAGATCAGGG ACCTCCTGGT 
CCTGATGGCC CAAGAGGAGC ACCTGGGCCT CCAGGCCTCC CTGGGAGTGT TGACCTTCTG 
AGAGGGGAGC CAGGTGACTG TGGTCTACCA GGGCCACCAG GTCCCCCTGG CCCACCAGGC 
CCTCCAGGAT ACAAAGGCTT TCCAGGATGT GATGGAAAAG ATGGCCAGAA AGGACCAATG 
GGATTCCCGG GACCGCAGGG ACCACATGGA TTTCCTGGGC CACCTGGAGA GAAGGGTTTA 
CCTGGACCTC CAGGGAGAAA AGGGCCCACT GGTCTTCCAG GTCCCAGAGG TGAACCGGGG 
CCACCTGCAG ATGTGGATGA CTGTCCCCGA ATCCCAGGCC TTCCTGGGGC GCCAGGCATG 
AGAGGACCAG AAGGAGCCAT GGGGCTCTCT GGAATGAGAG GCCCCCCAGG ACCAGGGTGC 
AAAGGAGAGC CTGGGCTGGA TGGCAGGAGG GGTGTGGATG GCGTCCCTGG GTCTCCTGGG 
CCTCCCGGAC GTAAAGGTGA CACAGGAGAA GACGGCTACC CTGGAGGACC AGGGCCTCCT 
GGTCCCATTG GGGATCCTGG GCCCAAAGGG TTTGGCCCTG GATACCTCGG TGGCTTCCTC 
CTGGTTCTCC ACAGTCAGAC GGACCAGGAG CCCACCTGCC CCCTGGGCAT GCCCAGGCTC 
TGGACTGGGT ATAGTCTGTT ATACCTGGAA GGGCAAGAGA AAGCTCACAA TCAAGACCTT 
GGTCTGGCAG GGTCTTGCCT TCCCGTGTTT AGCACGCTGC CCTTTGCCTA CTGCAACATC 
CACCAGGTGT GCCACTATGC CCAGAGAAAC GACAGATCCT ACTGGCTGGC CAGCGCTGCG 
CCCCTCCCCA TGATGCCACT CTCTGAAGAG GCGATCCGCC CCTATGTCAG CCGCTGTGCG 
GTATGCGAGG CCCCGGCCCA GGCGGTGGCG GTGCACAGCC AGGACAAGTC CATCCCCCCA 
TGTCCGCAGA CCTGGAGGAG CCTCTGGATC GGGTATTCAT TCCTGATGCA CACAGGAGCT 
GGGGACCAAG GAGGAGGGCA GGCCCTTATG TCACCTGGCA GCTGCCTGGA AGATTTCAGA 
GCAGCACCAT TCCTTGAATG CCAGGGCCGG CAGGGAACTT GCCACTTTTT TGCAAATAAG 
TATAGCTTCT GGCTCACAAC GGTGAAAGCA GACTTGCAGT TTTCCTCTGC TCCAGCACCA 
GACACCTTAA AAGAAAGCCA GGCCCAACGC CAGAAAATCA GCCGGTGCCA GGTCTGCGTG 
AAGTATAGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009442742.2
CDS	425..5416
Translation	MWSLHIVLMRCSFRLTKSLATDPWSLTLILFSVQYVYGSGKKYVGPCGGRDCSVCHCVPEKGSRGPPGPPGPQGPIGPLGAPGPIGLSGEKGMRGDHGPPGAAGDKGDKGSTGVPGFPGLDGIPGHPGPPGPRGKPGMSGHNGSRGDPGFPGGRGALGPGGPLGHPGEKGEKGNSVFILGAVKGIQGDRGDPGLPGLPGSWGAGGPAGPTGYPGEPGLVGPPGQPGRPGLKGNPGVGVKGQMGDPGEVGQQGSPGPTLLVEPPDFRLYKGEKGIKGIPGMVGLPGPPGRKGESDIGAKGEKGIPGFPGPRGDPGSYGSPGFPGLKGELGLVGDPGLFGLIGPKGDPGNRGHPGPPGVLVTPPLPLKGPPGDPGFPGRYGETGDVGPPGPPGLLGRPGEACAGMIGPPGPQGFPGLPGLPGEAGIPGRPDSAPGKPGKPGSPGLPGAPGLQGLPGSSVIYCSVGNPGPQGIKGKVGPPGGRGSKGEKGNEGLCACEPGPMGPPGPPGLPGRQGSKGDLGLPGWLGTKGDPGPPGAEGPPGLPGKHGASGPPGNKGAKGDMVVSRVKGHKGERGPDGPPGFPGQPGSHGRDGHAGEKGDPGPPGDHEDATPGGKGFPGPLGPPGKAGPLGPPGLGFPGPPGQRGHPGVPGHPGVRGPDGLKGQKGDTISCNVTYPGRHGPPGFDGPPGPKGFPGPQGAPGLSGSDGHKGRPGTPGTAEIPGPPGFRGDMGDPGFGGEKGSSPAGPPGPPGSPGVNGQKGIPGDPAFGHLGPPGKRGLSGVPGIKGPRGDPGCPGAEGPAGIPGFPGLKGPKGREGHAGFPGVPGPPGHSCERGAPGIPGQPGLPGYPGSPGAPGGKGQLGDVGPPGPAGMKGLPGLPGWPGAHGPPGLPGIPGPFGDDGLPGPPGPKGPQGLPGFPGFPGERGKPGAEGCPGTKGEPGEKGMSGLPGDRGLRGAKGAIGPPGDEGEMAIISQKGTPGEPGPPGDDGFPGERGDKGTPGMQGRRGEPGRYGPPGFQRGEPGLPGDQGEPGSPGPPGFSGIDGARGPKGNKGDPASHFGPPGPKGEPGSPGCPGHFGASGEQGLPGIQGPRGSPGRPGPPGSSGPPGCPGDHGMPGLRGQPGEMGDPGPRGLQGDPGIPGPPGIKGPSGSPGLNGLHGLKGQKGTKGASGLHDVGPPGPVGIPGLKGERGDPGSPGISPPGPRGEKGPPGPPGSSGPPGPAGATGRAPKDIPDPGPPGDQGPPGPDGPRGAPGPPGLPGSVDLLRGEPGDCGLPGPPGPPGPPGPPGYKGFPGCDGKDGQKGPMGFPGPQGPHGFPGPPGEKGLPGPPGRKGPTGLPGPRGEPGPPADVDDCPRIPGLPGAPGMRGPEGAMGLSGMRGPPGPGCKGEPGLDGRRGVDGVPGSPGPPGRKGDTGEDGYPGGPGPPGPIGDPGPKGFGPGYLGGFLLVLHSQTDQEPTCPLGMPRLWTGYSLLYLEGQEKAHNQDLGLAGSCLPVFSTLPFAYCNIHQVCHYAQRNDRSYWLASAAPLPMMPLSEEAIRPYVSRCAVCEAPAQAVAVHSQDKSIPPCPQTWRSLWIGYSFLMHTGAGDQGGGQALMSPGSCLEDFRAAPFLECQGRQGTCHFFANKYSFWLTTVKADLQFSSAPAPDTLKESQAQRQKISRCQVCVKYS

Target ORF information:

RefSeq Version	XM_009444467.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes collagen type IV alpha 4 (COL4A4), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009444467.2

ORF Insert Sequence:

ATGTGGTCTC TGCACATAGT ACTAATGAGG TGCTCCTTCA GATTGACCAA GTCCTTGGCC 
ACAGATCCCT GGTCACTTAC ACTCATTCTC TTTTCTGTAC AATATGTATA TGGGAGTGGA 
AAGAAATACG TTGGTCCTTG TGGAGGAAGA GATTGCTCTG TTTGCCACTG TGTTCCTGAA 
AAGGGGTCTC GGGGTCCACC AGGACCACCA GGGCCACAGG GTCCAATTGG ACCCCTGGGA 
GCCCCAGGAC CCATTGGGCT TTCAGGAGAG AAAGGAATGA GAGGGGACCA CGGCCCTCCT 
GGAGCAGCAG GGGACAAAGG AGATAAGGGT TCAACTGGTG TTCCTGGATT TCCAGGTTTA 
GATGGCATAC CTGGGCACCC AGGGCCTCCT GGACCCAGAG GCAAACCTGG TATGAGTGGC 
CACAATGGCT CAAGAGGTGA CCCAGGGTTT CCAGGAGGAA GAGGAGCTCT TGGCCCAGGA 
GGCCCCCTAG GCCATCCTGG GGAAAAGGGA GAAAAAGGAA ATTCAGTGTT CATTTTAGGT 
GCCGTTAAAG GTATTCAGGG AGACAGAGGG GACCCAGGAC TGCCTGGCTT ACCAGGATCT 
TGGGGGGCAG GAGGACCGGC GGGTCCCACA GGATATCCTG GAGAGCCAGG GTTAGTGGGA 
CCTCCGGGCC AACCAGGGCG TCCAGGTTTG AAGGGAAATC CCGGTGTGGG AGTAAAGGGG 
CAAATGGGAG ACCCGGGTGA GGTTGGTCAG CAAGGTTCTC CTGGACCCAC CCTGTTGGTG 
GAGCCACCTG ACTTTCGTCT CTATAAAGGA GAAAAGGGTA TAAAAGGAAT TCCTGGAATG 
GTTGGACTGC CAGGACCACC AGGACGCAAG GGAGAATCTG ATATTGGGGC AAAAGGAGAA 
AAAGGTATTC CTGGATTTCC AGGGCCTCGG GGGGATCCTG GTTCCTATGG ATCTCCAGGT 
TTTCCAGGAT TAAAGGGAGA ACTAGGACTG GTTGGAGATC CTGGGCTATT TGGATTAATT 
GGCCCAAAGG GGGATCCTGG AAATCGAGGG CACCCAGGAC CACCAGGTGT TTTGGTGACT 
CCACCTCTTC CACTCAAAGG CCCACCAGGG GACCCAGGGT TCCCTGGCCG CTATGGAGAA 
ACAGGGGATG TTGGACCACC TGGTCCCCCA GGTCTCTTGG GCAGACCAGG GGAAGCCTGT 
GCAGGCATGA TAGGACCCCC TGGGCCACAA GGATTTCCTG GTCTTCCTGG GCTTCCAGGA 
GAAGCTGGTA TTCCTGGGAG ACCTGATTCT GCTCCAGGAA AACCAGGGAA GCCAGGATCA 
CCTGGCTTGC CTGGAGCACC AGGCCTGCAG GGCCTCCCAG GATCAAGTGT GATATACTGT 
AGTGTTGGGA ATCCTGGACC ACAAGGAATA AAAGGCAAAG TGGGTCCCCC AGGAGGAAGA 
GGCTCAAAAG GAGAAAAAGG AAATGAAGGA CTCTGTGCCT GTGAGCCTGG ACCCATGGGC 
CCCCCTGGCC CTCCAGGACT TCCTGGGAGG CAGGGGAGTA AGGGAGACTT GGGGCTCCCT 
GGCTGGCTTG GAACAAAAGG TGACCCAGGA CCTCCTGGTG CTGAAGGACC TCCAGGGCTA 
CCAGGAAAGC ATGGTGCCTC TGGACCACCT GGCAACAAAG GGGCGAAGGG TGACATGGTT 
GTATCAAGAG TTAAAGGGCA CAAAGGAGAA AGAGGTCCTG ATGGGCCCCC AGGATTTCCA 
GGGCAGCCAG GATCACATGG TCGGGATGGA CATGCTGGAG AAAAAGGGGA TCCAGGACCC 
CCAGGGGATC ATGAAGATGC GACCCCAGGT GGTAAAGGAT TTCCTGGACC TCTGGGCCCC 
CCGGGCAAAG CAGGACCTTT GGGGCCCCCA GGACTGGGAT TTCCTGGTCC ACCAGGACAG 
CGAGGCCACC CAGGAGTTCC AGGCCACCCA GGTGTGAGGG GCCCTGATGG CTTGAAGGGT 
CAGAAAGGTG ACACAATTTC TTGCAACGTA ACCTACCCTG GGAGGCACGG CCCTCCAGGT 
TTTGATGGAC CTCCAGGTCC GAAGGGATTT CCAGGTCCCC AAGGTGCCCC TGGGCTGAGT 
GGTTCAGATG GGCATAAAGG CAGACCTGGC ACACCAGGAA CAGCGGAAAT ACCAGGTCCA 
CCTGGTTTTC GTGGTGACAT GGGAGATCCG GGTTTTGGAG GTGAAAAGGG GTCCTCCCCT 
GCTGGGCCCC CAGGCCCTCC CGGCTCACCA GGAGTGAATG GTCAGAAAGG AATCCCGGGA 
GACCCTGCAT TTGGTCACCT GGGACCCCCG GGAAAGAGGG GTCTTTCAGG AGTGCCAGGG 
ATAAAAGGAC CCAGAGGTGA TCCGGGATGT CCAGGGGCTG AAGGGCCAGC TGGCATTCCT 
GGATTCCCAG GTCTCAAAGG TCCCAAAGGC AGAGAGGGAC ATGCTGGGTT TCCAGGTGTC 
CCAGGTCCAC CTGGCCATTC CTGTGAAAGA GGTGCTCCAG GGATACCAGG GCAACCGGGA 
CTCCCTGGGT ATCCAGGTAG CCCAGGTGCT CCAGGTGGGA AAGGACAGCT GGGAGATGTG 
GGGCCTCCCG GGCCAGCTGG AATGAAAGGC CTCCCTGGAC TCCCAGGATG GCCTGGGGCA 
CATGGTCCCC CAGGCCTCCC AGGAATCCCA GGTCCCTTTG GAGATGATGG GCTACCTGGT 
CCTCCAGGTC CAAAGGGACC CCAGGGGCTG CCTGGTTTCC CAGGTTTTCC CGGAGAAAGA 
GGAAAGCCTG GTGCAGAGGG ATGTCCTGGC ACAAAGGGAG AACCTGGAGA GAAGGGCATG 
TCTGGCCTTC CCGGAGACCG GGGACTGAGA GGGGCCAAAG GAGCCATAGG ACCTCCCGGA 
GATGAAGGAG AAATGGCTAT CATTTCCCAA AAGGGAACAC CTGGGGAACC TGGACCTCCT 
GGAGATGATG GATTCCCAGG AGAAAGAGGT GATAAAGGAA CTCCCGGGAT GCAAGGGAGA 
AGAGGAGAGC CGGGAAGATA CGGACCACCT GGATTTCAGA GAGGGGAACC TGGACTTCCA 
GGTGACCAGG GTGAGCCAGG TTCTCCAGGT CCCCCTGGAT TTTCAGGAAT TGATGGAGCA 
AGAGGACCTA AAGGAAACAA AGGTGACCCT GCCAGTCACT TTGGTCCACC TGGTCCAAAG 
GGTGAGCCAG GTAGCCCTGG ATGTCCAGGG CATTTTGGAG CATCTGGAGA GCAGGGCTTG 
CCTGGTATTC AAGGGCCCAG AGGATCACCT GGAAGGCCAG GGCCACCTGG CTCCTCTGGA 
CCACCAGGGT GCCCAGGTGA TCACGGGATG CCTGGGCTGA GGGGACAGCC AGGAGAAATG 
GGAGACCCTG GGCCAAGAGG CCTCCAGGGG GATCCAGGGA TACCAGGTCC TCCGGGAATA 
AAAGGTCCCT CCGGATCACC TGGCCTGAAC GGCTTGCATG GATTGAAAGG TCAGAAAGGA 
ACTAAAGGTG CTTCAGGTTT GCATGATGTG GGGCCACCTG GTCCAGTGGG AATACCTGGG 
CTAAAAGGGG AGAGAGGAGA CCCTGGGAGC CCAGGAATCT CTCCTCCAGG TCCTCGTGGA 
GAAAAAGGTC CCCCAGGACC CCCAGGGAGT TCAGGACCAC CTGGTCCTGC AGGTGCCACA 
GGAAGAGCTC CTAAGGACAT TCCTGACCCG GGTCCACCTG GAGATCAGGG ACCTCCTGGT 
CCTGATGGCC CAAGAGGAGC ACCTGGGCCT CCAGGCCTCC CTGGGAGTGT TGACCTTCTG 
AGAGGGGAGC CAGGTGACTG TGGTCTACCA GGGCCACCAG GTCCCCCTGG CCCACCAGGC 
CCTCCAGGAT ACAAAGGCTT TCCAGGATGT GATGGAAAAG ATGGCCAGAA AGGACCAATG 
GGATTCCCGG GACCGCAGGG ACCACATGGA TTTCCTGGGC CACCTGGAGA GAAGGGTTTA 
CCTGGACCTC CAGGGAGAAA AGGGCCCACT GGTCTTCCAG GTCCCAGAGG TGAACCGGGG 
CCACCTGCAG ATGTGGATGA CTGTCCCCGA ATCCCAGGCC TTCCTGGGGC GCCAGGCATG 
AGAGGACCAG AAGGAGCCAT GGGGCTCTCT GGAATGAGAG GCCCCCCAGG ACCAGGGTGC 
AAAGGAGAGC CTGGGCTGGA TGGCAGGAGG GGTGTGGATG GCGTCCCTGG GTCTCCTGGG 
CCTCCCGGAC GTAAAGGTGA CACAGGAGAA GACGGCTACC CTGGAGGACC AGGGCCTCCT 
GGTCCCATTG GGGATCCTGG GCCCAAAGGG TTTGGCCCTG GATACCTCGG TGGCTTCCTC 
CTGGTTCTCC ACAGTCAGAC GGACCAGGAG CCCACCTGCC CCCTGGGCAT GCCCAGGCTC 
TGGACTGGGT ATAGTCTGTT ATACCTGGAA GGGCAAGAGA AAGCTCACAA TCAAGACCTT 
GGTCTGGCAG GGTCTTGCCT TCCCGTGTTT AGCACGCTGC CCTTTGCCTA CTGCAACATC 
CACCAGGTGT GCCACTATGC CCAGAGAAAC GACAGATCCT ACTGGCTGGC CAGCGCTGCG 
CCCCTCCCCA TGATGCCACT CTCTGAAGAG GCGATCCGCC CCTATGTCAG CCGCTGTGCG 
GTATGCGAGG CCCCGGCCCA GGCGGTGGCG GTGCACAGCC AGGACAAGTC CATCCCCCCA 
TGTCCGCAGA CCTGGAGGAG CCTCTGGATC GGGTATTCAT TCCTGATGCA CACAGGAGCT 
GGGGACCAAG GAGGAGGGCA GGCCCTTATG TCACCTGGCA GCTGCCTGGA AGATTTCAGA 
GCAGCACCAT TCCTTGAATG CCAGGGCCGG CAGGGAACTT GCCACTTTTT TGCAAATAAG 
TATAGCTTCT GGCTCACAAC GGTGAAAGCA GACTTGCAGT TTTCCTCTGC TCCAGCACCA 
GACACCTTAA AAGAAAGCCA GGCCCAACGC CAGAAAATCA GCCGGTGCCA GGTCTGCGTG 
AAGTATAGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL4A4 ( XM_016950592.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016806081.1 Pan troglodytes collagen type IV alpha 4 (COL4A4), transcript variant X7, mRNA.

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CloneID	OPc80766
Clone ID Related Accession (Same CDS sequence)	XM_016950592.1
Accession Version	XM_016950592.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4965bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	collagen alpha-4(IV) chain isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006470.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGTCTC TGCACATAGT ACTAATGAGG TGCTCCTTCA GATTGACCAA GTCCTTGGCC 
ACAGATCCCT GGTCACTTAC ACTCATTCTC TTTTCTGTAC AATATGTATA TGGGAGTGGA 
AAGAAATACG TTGGTCCTTG TGGAGGAAGA GATTGCTCTG TTTGCCACTG TGTTCCTGAA 
AAGGGGTCTC GGGGTCCACC AGGACCACCA GGGCCACAGG GTCCAATTGG ACCCCTGGGA 
GCCCCAGGAC CCATTGGGCT TTCAGGAGAG AAAGGAATGA GAGGGGACCA CGGCCCTCCT 
GGAGCAGCAG GGGACAAAGG AGATAAGGGT TCAACTGGTG TTCCTGGATT TCCAGGTTTA 
GATGGCATAC CTGGGCACCC AGGGCCTCCT GGACCCAGAG GCAAACCTGG TATGAGTGGC 
CACAATGGCT CAAGAGGTGA CCCAGGGTTT CCAGGAGGAA GAGGAGCTCT TGGCCCAGGA 
GGCCCCCTAG GCCATCCTGG GGAAAAGGGA GAAAAAGGAA ATTCAGTGTT CATTTTAGGT 
GCCGTTAAAG GTATTCAGGG AGACAGAGGG GACCCAGGAC TGCCTGGCTT ACCAGGATCT 
TGGGGGGCAG GAGGACCGGC GGGTCCCACA GGATATCCTG GAGAGCCAGG GTTAGTGGGA 
CCTCCGGGCC AACCAGGGCG TCCAGGTTTG AAGGGAAATC CCGGTGTGGG AGTAAAGGGG 
CAAATGGGAG ACCCGGGTGA GGTTGGTCAG CAAGGTTCTC CTGGACCCAC CCTGTTGGTG 
GAGCCACCTG ACTTTCGTCT CTATAAAGGA GAAAAGGGTA TAAAAGGAAT TCCTGGAATG 
GTTGGACTGC CAGGACCACC AGGACGCAAG GGAGAATCTG ATATTGGGGC AAAAGGAGAA 
AAAGGTATTC CTGGATTTCC AGGGCCTCGG GGGGATCCTG GTTCCTATGG ATCTCCAGGT 
TTTCCAGGAT TAAAGGGAGA ACTAGGACTG GTTGGAGATC CTGGGCTATT TGGATTAATT 
GGCCCAAAGG GGGATCCTGG AAATCGAGGG CACCCAGGAC CACCAGGTGT TTTGGTGACT 
CCACCTCTTC CACTCAAAGG CCCACCAGGG GACCCAGGGT TCCCTGGCCG CTATGGAGAA 
ACAGGGGATG TTGGACCACC TGGTCCCCCA GGTCTCTTGG GCAGACCAGG GGAAGCCTGT 
GCAGGCATGA TAGGACCCCC TGGGCCACAA GGATTTCCTG GTCTTCCTGG GCTTCCAGGA 
GAAGCTGGTA TTCCTGGGAG ACCTGATTCT GCTCCAGGAA AACCAGGGAA GCCAGGATCA 
CCTGGCTTGC CTGGAGCACC AGGCCTGCAG GGCCTCCCAG GATCAAGTGT GATATACTGT 
AGTGTTGGGA ATCCTGGACC ACAAGGAATA AAAGGCAAAG TGGGTCCCCC AGGAGGAAGA 
GGCTCAAAAG GAGAAAAAGG AAATGAAGGA CTCTGTGCCT GTGAGCCTGG ACCCATGGGC 
CCCCCTGGCC CTCCAGGACT TCCTGGGAGG CAGGGGAGTA AGGGAGACTT GGGGCTCCCT 
GGCTGGCTTG GAACAAAAGG TGACCCAGGA CCTCCTGGTG CTGAAGGACC TCCAGGGCTA 
CCAGGAAAGC ATGGTGCCTC TGGACCACCT GGCAACAAAG GGGCGAAGGG TGACATGGTT 
GTATCAAGAG TTAAAGGGCA CAAAGGAGAA AGAGGTCCTG ATGGGCCCCC AGGATTTCCA 
GGGCAGCCAG GATCACATGG TCGGGATGGA CATGCTGGAG AAAAAGGGGA TCCAGGACCC 
CCAGGGGATC ATGAAGATGC GACCCCAGGT GGTAAAGGAT TTCCTGGACC TCTGGGCCCC 
CCGGGCAAAG CAGGACCTTT GGGGCCCCCA GGACTGGGAT TTCCTGGTCC ACCAGGACAG 
CGAGGCCACC CAGGAGTTCC AGGCCACCCA GGTGTGAGGG GCCCTGATGG CTTGAAGGGT 
CAGAAAGGTG ACACAATTTC TTGCAACGTA ACCTACCCTG GGAGGCACGG CCCTCCAGGT 
TTTGATGGAC CTCCAGGTTT TCGTGGTGAC ATGGGAGATC CGGGTTTTGG AGGTGAAAAG 
GGGTCCTCCC CTGCTGGGCC CCCAGGCCCT CCCGGCTCAC CAGGAGTGAA TGGTCAGAAA 
GGAATCCCGG GAGACCCTGC ATTTGGTCAC CTGGGACCCC CGGGAAAGAG GGGTCTTTCA 
GGAGTGCCAG GGATAAAAGG ACCCAGAGGT GATCCGGGAT GTCCAGGGGC TGAAGGGCCA 
GCTGGCATTC CTGGATTCCC AGGTCTCAAA GGTCCCAAAG GCAGAGAGGG ACATGCTGGG 
TTTCCAGGTG TCCCAGGTCC ACCTGGCCAT TCCTGTGAAA GAGGTGCTCC AGGGATACCA 
GGGCAACCGG GACTCCCTGG GTATCCAGGT AGCCCAGGTG CTCCAGGTGG GAAAGGACAG 
CTGGGAGATG TGGGGCCTCC CGGGCCAGCT GGAATGAAAG GCCTCCCTGG ACTCCCAGGA 
TGGCCTGGGG CACATGGTCC CCCAGGCCTC CCAGGAATCC CAGGTCCCTT TGGAGATGAT 
GGGCTACCTG GTCCTCCAGG TCCAAAGGGA CCCCAGGGGC TGCCTGGTTT CCCAGGTTTT 
CCCGGAGAAA GAGGAAAGCC TGGTGCAGAG GGATGTCCTG GCACAAAGGG AGAACCTGGA 
GAGAAGGGCA TGTCTGGCCT TCCCGGAGAC CGGGGACTGA GAGGGGCCAA AGGAGCCATA 
GGACCTCCCG GAGATGAAGG AGAAATGGCT ATCATTTCCC AAAAGGGAAC ACCTGGGGAA 
CCTGGACCTC CTGGAGATGA TGGATTCCCA GGAGAAAGAG GTGATAAAGG AACTCCCGGG 
ATGCAAGGGA GAAGAGGAGA GCCGGGAAGA TACGGACCAC CTGGATTTCA GAGAGGGGAA 
CCTGGTGAGA AAGGTCAGCC AGGGCATCCT GGACCCCCAG GCCCTCCAGG CTCAACTGGT 
CTAAGAGGGT TCATTGGTTT TCCAGGACTT CCAGGTGACC AGGGTGAGCC AGGTTCTCCA 
GGTCCCCCTG GATTTTCAGG AATTGATGGA GCAAGAGGAC CTAAAGGAAA CAAAGGTGAC 
CCTGCCAGTC ACTTTGGTCC ACCTGGTCCA AAGGGTGAGC CAGGTAGCCC TGGATGTCCA 
GGGCATTTTG GAGCATCTGG AGAGCAGGGC TTGCCTGGTA TTCAAGGGCC CAGAGGATCA 
CCTGGAAGGC CAGGGCCACC TGGCTCCTCT GGACCACCAG GGTGCCCAGG TGATCACGGG 
ATGCCTGGGC TGAGGGGACA GCCAGGAGAA ATGGGAGACC CTGGGCCAAG AGGCCTCCAG 
GGGGATCCAG GGATACCAGG TCCTCCGGGA ATAAAAGGTC CCTCCGGATC ACCTGGCCTG 
AACGGCTTGC ATGGATTGAA AGGTCAGAAA GGAACTAAAG GTGCTTCAGG TTTGCATGAT 
GTGGGGCCAC CTGGTCCAGT GGGAATACCT GGGCTAAAAG GGGAGAGAGG AGACCCTGGG 
AGCCCAGGAA TCTCTCCTCC AGGTCCTCGT GGAGAAAAAG GTCCCCCAGG ACCCCCAGGG 
AGTTCAGGAC CACCTGGTCC TGCAGGTGCC ACAGGAAGAG CTCCTAAGGA CATTCCTGAC 
CCGGGTCCAC CTGGAGATCA GGGACCTCCT GGTCCTGATG GCCCAAGAGG AGCACCTGGG 
CCTCCAGGCC TCCCTGGGAG TGTTGACCTT CTGAGAGGGG AGCCAGGTGA CTGTGGTCTA 
CCAGGGCCAC CAGGTCCCCC TGGCCCACCA GGCCCTCCAG GATACAAAGG CTTTCCAGGA 
TGTGATGGAA AAGATGGCCA GAAAGGACCA ATGGGATTCC CGGGACCGCA GGGACCACAT 
GGATTTCCTG GGCCACCTGG AGAGAAGGGT TTACCTGGAC CTCCAGGGAG AAAAGGGCCC 
ACTGGTCTTC CAGGTCCCAG AGGTGAACCG GGGCCACCTG CAGATGTGGA TGACTGTCCC 
CGAATCCCAG GCCTTCCTGG GGCGCCAGGC ATGAGAGGAC CAGAAGGAGC CATGGGGCTC 
TCTGGAATGA GAGGCCCCCC AGGACCAGGG TGCAAAGGAG AGCCTGGGCT GGATGGCAGG 
AGGGGTGTGG ATGGCGTCCC TGGGTCTCCT GGGCCTCCCG GACGTAAAGG TGACACAGGA 
GAAGACGGCT ACCCTGGAGG ACCAGGGCCT CCTGGTCCCA TTGGGGATCC TGGGCCCAAA 
GGGTTTGGCC CTGGATACCT CGGTGGCTTC CTCCTGGTTC TCCACAGTCA GACGGACCAG 
GAGCCCACCT GCCCCCTGGG CATGCCCAGG CTCTGGACTG GGTATAGTCT GTTATACCTG 
GAAGGGCAAG AGAAAGCTCA CAATCAAGAC CTTGGTCTGG CAGGGTCTTG CCTTCCCGTG 
TTTAGCACGC TGCCCTTTGC CTACTGCAAC ATCCACCAGG TGTGCCACTA TGCCCAGAGA 
AACGACAGAT CCTACTGGCT GGCCAGCGCT GCGCCCCTCC CCATGATGCC ACTCTCTGAA 
GAGGCGATCC GCCCCTATGT CAGCCGCTGT GCGGTATGCG AGGCCCCGGC CCAGGCGGTG 
GCGGTGCACA GCCAGGACAA GTCCATCCCC CCATGTCCGC AGACCTGGAG GAGCCTCTGG 
ATCGGGTATT CATTCCTGAT GCACACAGGA GCTGGGGACC AAGGAGGAGG GCAGGCCCTT 
ATGTCACCTG GCAGCTGCCT GGAAGATTTC AGAGCAGCAC CATTCCTTGA ATGCCAGGGC 
CGGCAGGGAA CTTGCCACTT TTTTGCAAAT AAGTATAGCT TCTGGCTCAC AACGGTGAAA 
GCAGACTTGC AGTTTTCCTC TGCTCCAGCA CCAGACACCT TAAAAGAAAG CCAGGCCCAA 
CGCCAGAAAA TCAGCCGGTG CCAGGTCTGC GTGAAGTATA GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016806081.1
CDS	425..5389
Translation	MWSLHIVLMRCSFRLTKSLATDPWSLTLILFSVQYVYGSGKKYVGPCGGRDCSVCHCVPEKGSRGPPGPPGPQGPIGPLGAPGPIGLSGEKGMRGDHGPPGAAGDKGDKGSTGVPGFPGLDGIPGHPGPPGPRGKPGMSGHNGSRGDPGFPGGRGALGPGGPLGHPGEKGEKGNSVFILGAVKGIQGDRGDPGLPGLPGSWGAGGPAGPTGYPGEPGLVGPPGQPGRPGLKGNPGVGVKGQMGDPGEVGQQGSPGPTLLVEPPDFRLYKGEKGIKGIPGMVGLPGPPGRKGESDIGAKGEKGIPGFPGPRGDPGSYGSPGFPGLKGELGLVGDPGLFGLIGPKGDPGNRGHPGPPGVLVTPPLPLKGPPGDPGFPGRYGETGDVGPPGPPGLLGRPGEACAGMIGPPGPQGFPGLPGLPGEAGIPGRPDSAPGKPGKPGSPGLPGAPGLQGLPGSSVIYCSVGNPGPQGIKGKVGPPGGRGSKGEKGNEGLCACEPGPMGPPGPPGLPGRQGSKGDLGLPGWLGTKGDPGPPGAEGPPGLPGKHGASGPPGNKGAKGDMVVSRVKGHKGERGPDGPPGFPGQPGSHGRDGHAGEKGDPGPPGDHEDATPGGKGFPGPLGPPGKAGPLGPPGLGFPGPPGQRGHPGVPGHPGVRGPDGLKGQKGDTISCNVTYPGRHGPPGFDGPPGFRGDMGDPGFGGEKGSSPAGPPGPPGSPGVNGQKGIPGDPAFGHLGPPGKRGLSGVPGIKGPRGDPGCPGAEGPAGIPGFPGLKGPKGREGHAGFPGVPGPPGHSCERGAPGIPGQPGLPGYPGSPGAPGGKGQLGDVGPPGPAGMKGLPGLPGWPGAHGPPGLPGIPGPFGDDGLPGPPGPKGPQGLPGFPGFPGERGKPGAEGCPGTKGEPGEKGMSGLPGDRGLRGAKGAIGPPGDEGEMAIISQKGTPGEPGPPGDDGFPGERGDKGTPGMQGRRGEPGRYGPPGFQRGEPGEKGQPGHPGPPGPPGSTGLRGFIGFPGLPGDQGEPGSPGPPGFSGIDGARGPKGNKGDPASHFGPPGPKGEPGSPGCPGHFGASGEQGLPGIQGPRGSPGRPGPPGSSGPPGCPGDHGMPGLRGQPGEMGDPGPRGLQGDPGIPGPPGIKGPSGSPGLNGLHGLKGQKGTKGASGLHDVGPPGPVGIPGLKGERGDPGSPGISPPGPRGEKGPPGPPGSSGPPGPAGATGRAPKDIPDPGPPGDQGPPGPDGPRGAPGPPGLPGSVDLLRGEPGDCGLPGPPGPPGPPGPPGYKGFPGCDGKDGQKGPMGFPGPQGPHGFPGPPGEKGLPGPPGRKGPTGLPGPRGEPGPPADVDDCPRIPGLPGAPGMRGPEGAMGLSGMRGPPGPGCKGEPGLDGRRGVDGVPGSPGPPGRKGDTGEDGYPGGPGPPGPIGDPGPKGFGPGYLGGFLLVLHSQTDQEPTCPLGMPRLWTGYSLLYLEGQEKAHNQDLGLAGSCLPVFSTLPFAYCNIHQVCHYAQRNDRSYWLASAAPLPMMPLSEEAIRPYVSRCAVCEAPAQAVAVHSQDKSIPPCPQTWRSLWIGYSFLMHTGAGDQGGGQALMSPGSCLEDFRAAPFLECQGRQGTCHFFANKYSFWLTTVKADLQFSSAPAPDTLKESQAQRQKISRCQVCVKYS

Target ORF information:

RefSeq Version	XM_016950592.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes collagen type IV alpha 4 (COL4A4), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016950592.1

ORF Insert Sequence:

ATGTGGTCTC TGCACATAGT ACTAATGAGG TGCTCCTTCA GATTGACCAA GTCCTTGGCC 
ACAGATCCCT GGTCACTTAC ACTCATTCTC TTTTCTGTAC AATATGTATA TGGGAGTGGA 
AAGAAATACG TTGGTCCTTG TGGAGGAAGA GATTGCTCTG TTTGCCACTG TGTTCCTGAA 
AAGGGGTCTC GGGGTCCACC AGGACCACCA GGGCCACAGG GTCCAATTGG ACCCCTGGGA 
GCCCCAGGAC CCATTGGGCT TTCAGGAGAG AAAGGAATGA GAGGGGACCA CGGCCCTCCT 
GGAGCAGCAG GGGACAAAGG AGATAAGGGT TCAACTGGTG TTCCTGGATT TCCAGGTTTA 
GATGGCATAC CTGGGCACCC AGGGCCTCCT GGACCCAGAG GCAAACCTGG TATGAGTGGC 
CACAATGGCT CAAGAGGTGA CCCAGGGTTT CCAGGAGGAA GAGGAGCTCT TGGCCCAGGA 
GGCCCCCTAG GCCATCCTGG GGAAAAGGGA GAAAAAGGAA ATTCAGTGTT CATTTTAGGT 
GCCGTTAAAG GTATTCAGGG AGACAGAGGG GACCCAGGAC TGCCTGGCTT ACCAGGATCT 
TGGGGGGCAG GAGGACCGGC GGGTCCCACA GGATATCCTG GAGAGCCAGG GTTAGTGGGA 
CCTCCGGGCC AACCAGGGCG TCCAGGTTTG AAGGGAAATC CCGGTGTGGG AGTAAAGGGG 
CAAATGGGAG ACCCGGGTGA GGTTGGTCAG CAAGGTTCTC CTGGACCCAC CCTGTTGGTG 
GAGCCACCTG ACTTTCGTCT CTATAAAGGA GAAAAGGGTA TAAAAGGAAT TCCTGGAATG 
GTTGGACTGC CAGGACCACC AGGACGCAAG GGAGAATCTG ATATTGGGGC AAAAGGAGAA 
AAAGGTATTC CTGGATTTCC AGGGCCTCGG GGGGATCCTG GTTCCTATGG ATCTCCAGGT 
TTTCCAGGAT TAAAGGGAGA ACTAGGACTG GTTGGAGATC CTGGGCTATT TGGATTAATT 
GGCCCAAAGG GGGATCCTGG AAATCGAGGG CACCCAGGAC CACCAGGTGT TTTGGTGACT 
CCACCTCTTC CACTCAAAGG CCCACCAGGG GACCCAGGGT TCCCTGGCCG CTATGGAGAA 
ACAGGGGATG TTGGACCACC TGGTCCCCCA GGTCTCTTGG GCAGACCAGG GGAAGCCTGT 
GCAGGCATGA TAGGACCCCC TGGGCCACAA GGATTTCCTG GTCTTCCTGG GCTTCCAGGA 
GAAGCTGGTA TTCCTGGGAG ACCTGATTCT GCTCCAGGAA AACCAGGGAA GCCAGGATCA 
CCTGGCTTGC CTGGAGCACC AGGCCTGCAG GGCCTCCCAG GATCAAGTGT GATATACTGT 
AGTGTTGGGA ATCCTGGACC ACAAGGAATA AAAGGCAAAG TGGGTCCCCC AGGAGGAAGA 
GGCTCAAAAG GAGAAAAAGG AAATGAAGGA CTCTGTGCCT GTGAGCCTGG ACCCATGGGC 
CCCCCTGGCC CTCCAGGACT TCCTGGGAGG CAGGGGAGTA AGGGAGACTT GGGGCTCCCT 
GGCTGGCTTG GAACAAAAGG TGACCCAGGA CCTCCTGGTG CTGAAGGACC TCCAGGGCTA 
CCAGGAAAGC ATGGTGCCTC TGGACCACCT GGCAACAAAG GGGCGAAGGG TGACATGGTT 
GTATCAAGAG TTAAAGGGCA CAAAGGAGAA AGAGGTCCTG ATGGGCCCCC AGGATTTCCA 
GGGCAGCCAG GATCACATGG TCGGGATGGA CATGCTGGAG AAAAAGGGGA TCCAGGACCC 
CCAGGGGATC ATGAAGATGC GACCCCAGGT GGTAAAGGAT TTCCTGGACC TCTGGGCCCC 
CCGGGCAAAG CAGGACCTTT GGGGCCCCCA GGACTGGGAT TTCCTGGTCC ACCAGGACAG 
CGAGGCCACC CAGGAGTTCC AGGCCACCCA GGTGTGAGGG GCCCTGATGG CTTGAAGGGT 
CAGAAAGGTG ACACAATTTC TTGCAACGTA ACCTACCCTG GGAGGCACGG CCCTCCAGGT 
TTTGATGGAC CTCCAGGTTT TCGTGGTGAC ATGGGAGATC CGGGTTTTGG AGGTGAAAAG 
GGGTCCTCCC CTGCTGGGCC CCCAGGCCCT CCCGGCTCAC CAGGAGTGAA TGGTCAGAAA 
GGAATCCCGG GAGACCCTGC ATTTGGTCAC CTGGGACCCC CGGGAAAGAG GGGTCTTTCA 
GGAGTGCCAG GGATAAAAGG ACCCAGAGGT GATCCGGGAT GTCCAGGGGC TGAAGGGCCA 
GCTGGCATTC CTGGATTCCC AGGTCTCAAA GGTCCCAAAG GCAGAGAGGG ACATGCTGGG 
TTTCCAGGTG TCCCAGGTCC ACCTGGCCAT TCCTGTGAAA GAGGTGCTCC AGGGATACCA 
GGGCAACCGG GACTCCCTGG GTATCCAGGT AGCCCAGGTG CTCCAGGTGG GAAAGGACAG 
CTGGGAGATG TGGGGCCTCC CGGGCCAGCT GGAATGAAAG GCCTCCCTGG ACTCCCAGGA 
TGGCCTGGGG CACATGGTCC CCCAGGCCTC CCAGGAATCC CAGGTCCCTT TGGAGATGAT 
GGGCTACCTG GTCCTCCAGG TCCAAAGGGA CCCCAGGGGC TGCCTGGTTT CCCAGGTTTT 
CCCGGAGAAA GAGGAAAGCC TGGTGCAGAG GGATGTCCTG GCACAAAGGG AGAACCTGGA 
GAGAAGGGCA TGTCTGGCCT TCCCGGAGAC CGGGGACTGA GAGGGGCCAA AGGAGCCATA 
GGACCTCCCG GAGATGAAGG AGAAATGGCT ATCATTTCCC AAAAGGGAAC ACCTGGGGAA 
CCTGGACCTC CTGGAGATGA TGGATTCCCA GGAGAAAGAG GTGATAAAGG AACTCCCGGG 
ATGCAAGGGA GAAGAGGAGA GCCGGGAAGA TACGGACCAC CTGGATTTCA GAGAGGGGAA 
CCTGGTGAGA AAGGTCAGCC AGGGCATCCT GGACCCCCAG GCCCTCCAGG CTCAACTGGT 
CTAAGAGGGT TCATTGGTTT TCCAGGACTT CCAGGTGACC AGGGTGAGCC AGGTTCTCCA 
GGTCCCCCTG GATTTTCAGG AATTGATGGA GCAAGAGGAC CTAAAGGAAA CAAAGGTGAC 
CCTGCCAGTC ACTTTGGTCC ACCTGGTCCA AAGGGTGAGC CAGGTAGCCC TGGATGTCCA 
GGGCATTTTG GAGCATCTGG AGAGCAGGGC TTGCCTGGTA TTCAAGGGCC CAGAGGATCA 
CCTGGAAGGC CAGGGCCACC TGGCTCCTCT GGACCACCAG GGTGCCCAGG TGATCACGGG 
ATGCCTGGGC TGAGGGGACA GCCAGGAGAA ATGGGAGACC CTGGGCCAAG AGGCCTCCAG 
GGGGATCCAG GGATACCAGG TCCTCCGGGA ATAAAAGGTC CCTCCGGATC ACCTGGCCTG 
AACGGCTTGC ATGGATTGAA AGGTCAGAAA GGAACTAAAG GTGCTTCAGG TTTGCATGAT 
GTGGGGCCAC CTGGTCCAGT GGGAATACCT GGGCTAAAAG GGGAGAGAGG AGACCCTGGG 
AGCCCAGGAA TCTCTCCTCC AGGTCCTCGT GGAGAAAAAG GTCCCCCAGG ACCCCCAGGG 
AGTTCAGGAC CACCTGGTCC TGCAGGTGCC ACAGGAAGAG CTCCTAAGGA CATTCCTGAC 
CCGGGTCCAC CTGGAGATCA GGGACCTCCT GGTCCTGATG GCCCAAGAGG AGCACCTGGG 
CCTCCAGGCC TCCCTGGGAG TGTTGACCTT CTGAGAGGGG AGCCAGGTGA CTGTGGTCTA 
CCAGGGCCAC CAGGTCCCCC TGGCCCACCA GGCCCTCCAG GATACAAAGG CTTTCCAGGA 
TGTGATGGAA AAGATGGCCA GAAAGGACCA ATGGGATTCC CGGGACCGCA GGGACCACAT 
GGATTTCCTG GGCCACCTGG AGAGAAGGGT TTACCTGGAC CTCCAGGGAG AAAAGGGCCC 
ACTGGTCTTC CAGGTCCCAG AGGTGAACCG GGGCCACCTG CAGATGTGGA TGACTGTCCC 
CGAATCCCAG GCCTTCCTGG GGCGCCAGGC ATGAGAGGAC CAGAAGGAGC CATGGGGCTC 
TCTGGAATGA GAGGCCCCCC AGGACCAGGG TGCAAAGGAG AGCCTGGGCT GGATGGCAGG 
AGGGGTGTGG ATGGCGTCCC TGGGTCTCCT GGGCCTCCCG GACGTAAAGG TGACACAGGA 
GAAGACGGCT ACCCTGGAGG ACCAGGGCCT CCTGGTCCCA TTGGGGATCC TGGGCCCAAA 
GGGTTTGGCC CTGGATACCT CGGTGGCTTC CTCCTGGTTC TCCACAGTCA GACGGACCAG 
GAGCCCACCT GCCCCCTGGG CATGCCCAGG CTCTGGACTG GGTATAGTCT GTTATACCTG 
GAAGGGCAAG AGAAAGCTCA CAATCAAGAC CTTGGTCTGG CAGGGTCTTG CCTTCCCGTG 
TTTAGCACGC TGCCCTTTGC CTACTGCAAC ATCCACCAGG TGTGCCACTA TGCCCAGAGA 
AACGACAGAT CCTACTGGCT GGCCAGCGCT GCGCCCCTCC CCATGATGCC ACTCTCTGAA 
GAGGCGATCC GCCCCTATGT CAGCCGCTGT GCGGTATGCG AGGCCCCGGC CCAGGCGGTG 
GCGGTGCACA GCCAGGACAA GTCCATCCCC CCATGTCCGC AGACCTGGAG GAGCCTCTGG 
ATCGGGTATT CATTCCTGAT GCACACAGGA GCTGGGGACC AAGGAGGAGG GCAGGCCCTT 
ATGTCACCTG GCAGCTGCCT GGAAGATTTC AGAGCAGCAC CATTCCTTGA ATGCCAGGGC 
CGGCAGGGAA CTTGCCACTT TTTTGCAAAT AAGTATAGCT TCTGGCTCAC AACGGTGAAA 
GCAGACTTGC AGTTTTCCTC TGCTCCAGCA CCAGACACCT TAAAAGAAAG CCAGGCCCAA 
CGCCAGAAAA TCAGCCGGTG CCAGGTCTGC GTGAAGTATA GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL4A4 ( XM_009444468.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_009442743.2 Pan troglodytes collagen type IV alpha 4 (COL4A4), transcript variant X8, mRNA.

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CloneID	OPc80767
Clone ID Related Accession (Same CDS sequence)	XM_009444468.2
Accession Version	XM_009444468.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4884bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	collagen alpha-4(IV) chain isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006470.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 2, 2016 this sequence version replaced XM_009444468.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGTCTC TGCACATAGT ACTAATGAGG TGCTCCTTCA GATTGACCAA GTCCTTGGCC 
ACAGATCCCT GGTCACTTAC ACTCATTCTC TTTTCTGTAC AATATGTATA TGGGAGTGGA 
AAGAAATACG TTGGTCCTTG TGGAGGAAGA GATTGCTCTG TTTGCCACTG TGTTCCTGAA 
AAGGGGTCTC GGGGTCCACC AGGACCACCA GGGCCACAGG GTCCAATTGG ACCCCTGGGA 
GCCCCAGGAC CCATTGGGCT TTCAGGAGAG AAAGGAATGA GAGGGGACCA CGGCCCTCCT 
GGAGCAGCAG GGGACAAAGG AGATAAGGGT TCAACTGGTG TTCCTGGATT TCCAGGTTTA 
GATGGCATAC CTGGGCACCC AGGGCCTCCT GGACCCAGAG GCAAACCTGG TATGAGTGGC 
CACAATGGCT CAAGAGGTGA CCCAGGGTTT CCAGGAGGAA GAGGAGCTCT TGGCCCAGGA 
GGCCCCCTAG GCCATCCTGG GGAAAAGGGA GAAAAAGGAA ATTCAGTGTT CATTTTAGGT 
GCCGTTAAAG GTATTCAGGG AGACAGAGGG GACCCAGGAC TGCCTGGCTT ACCAGGATCT 
TGGGGGGCAG GAGGACCGGC GGGTCCCACA GGATATCCTG GAGAGCCAGG GTTAGTGGGA 
CCTCCGGGCC AACCAGGGCG TCCAGGTTTG AAGGGAAATC CCGGTGTGGG AGTAAAGGGG 
CAAATGGGAG ACCCGGGTGA GGTTGGTCAG CAAGGTTCTC CTGGACCCAC CCTGTTGGTG 
GAGCCACCTG ACTTTCGTCT CTATAAAGGA GAAAAGGGTA TAAAAGGAAT TCCTGGAATG 
GTTGGACTGC CAGGACCACC AGGACGCAAG GGAGAATCTG ATATTGGGGC AAAAGGAGAA 
AAAGGTATTC CTGGATTTCC AGGGCCTCGG GGGGATCCTG GTTCCTATGG ATCTCCAGGT 
TTTCCAGGAT TAAAGGGAGA ACTAGGACTG GTTGGAGATC CTGGGCTATT TGGATTAATT 
GGCCCAAAGG GGGATCCTGG AAATCGAGGG CACCCAGGAC CACCAGGTGT TTTGGTGACT 
CCACCTCTTC CACTCAAAGG CCCACCAGGG GACCCAGGGT TCCCTGGCCG CTATGGAGAA 
ACAGGGGATG TTGGACCACC TGGTCCCCCA GGTCTCTTGG GCAGACCAGG GGAAGCCTGT 
GCAGGCATGA TAGGACCCCC TGGGCCACAA GGATTTCCTG GTCTTCCTGG GCTTCCAGGA 
GAAGCTGGTA TTCCTGGGAG ACCTGATTCT GCTCCAGGAA AACCAGGGAA GCCAGGATCA 
CCTGGCTTGC CTGGAGCACC AGGCCTGCAG GGCCTCCCAG GATCAAGTGT GATATACTGT 
AGTGTTGGGA ATCCTGGACC ACAAGGAATA AAAGGCAAAG TGGGTCCCCC AGGAGGAAGA 
GGCTCAAAAG GAGAAAAAGG AAATGAAGGA CTCTGTGCCT GTGAGCCTGG ACCCATGGGC 
CCCCCTGGCC CTCCAGGACT TCCTGGGAGG CAGGGGAGTA AGGGAGACTT GGGGCTCCCT 
GGCTGGCTTG GAACAAAAGG TGACCCAGGA CCTCCTGGTG CTGAAGGACC TCCAGGGCTA 
CCAGGAAAGC ATGGTGCCTC TGGACCACCT GGCAACAAAG GGGCGAAGGG TGACATGGTT 
GTATCAAGAG TTAAAGGGCA CAAAGGAGAA AGAGGTCCTG ATGGGCCCCC AGGATTTCCA 
GGGCAGCCAG GATCACATGG TCGGGATGGA CATGCTGGAG AAAAAGGGGA TCCAGGACCC 
CCAGGGGATC ATGAAGATGC GACCCCAGGT GGTAAAGGAT TTCCTGGACC TCTGGGCCCC 
CCGGGCAAAG CAGGACCTTT GGGGCCCCCA GGACTGGGAT TTCCTGGTCC ACCAGGACAG 
CGAGGCCACC CAGGAGTTCC AGGCCACCCA GGTGTGAGGG GCCCTGATGG CTTGAAGGGT 
CAGAAAGGTG ACACAATTTC TTGCAACGTA ACCTACCCTG GGAGGCACGG CCCTCCAGGT 
TTTGATGGAC CTCCAGGTCC GAAGGGATTT CCAGGTCCCC AAGGTGCCCC TGGGCTGAGT 
GGTTCAGATG GGCATAAAGG CAGACCTGGC ACACCAGGAA CAGCGGAAAT ACCAGGTCCA 
CCTGGTTTTC GTGGTGACAT GGGAGATCCG GGTTTTGGAG GTGAAAAGGG GTCCTCCCCT 
GCTGGGCCCC CAGGCCCTCC CGGCTCACCA GGAGTGAATG GTCAGAAAGG AATCCCGGGA 
GACCCTGCAT TTGGTCACCT GGGACCCCCG GGAAAGAGGG GTCTTTCAGG AGTGCCAGGG 
ATAAAAGGAC CCAGAGGTGA TCCGGGATGT CCAGGGGCTG AAGGGCCAGC TGGCATTCCT 
GGATTCCCAG GTCTCAAAGG TCCCAAAGGC AGAGAGGGAC ATGCTGGGTT TCCAGGTGTC 
CCAGGTCCAC CTGGCCATTC CTGTGAAAGA GGTGCTCCAG GGATACCAGG GCAACCGGGA 
CTCCCTGGGT ATCCAGGTAG CCCAGGTGCT CCAGGTGGGA AAGGACAGCT GGGAGATGTG 
GGGCCTCCCG GGCCAGCTGG AATGAAAGGC CTCCCTGGAC TCCCAGGATG GCCTGGGGCA 
CATGGTCCCC CAGGCCTCCC AGGAATCCCA GGTCCCTTTG GAGATGATGG GCTACCTGGT 
CCTCCAGGTC CAAAGGGACC CCAGGGGCTG CCTGGTTTCC CAGGTTTTCC CGGAGAAAGA 
GGAAAGCCTG GTGCAGAGGG ATGTCCTGGC ACAAAGGGAG AACCTGGAGA GAAGGGCATG 
TCTGGCCTTC CCGGAGACCG GGGACTGAGA GGGGCCAAAG GAGCCATAGG ACCTCCCGGA 
GATGAAGGAG AAATGGCTAT CATTTCCCAA AAGGGAACAC CTGGGGAACC TGGACCTCCT 
GGAGATGATG GATTCCCAGG AGAAAGAGGT GATAAAGGAA CTCCCGGGAT GCAAGGGAGA 
AGAGGAGAGC CGGGAAGATA CGGACCACCT GGATTTCAGA GAGGGGAACC TGGTGAGAAA 
GGTCAGCCAG GGCATCCTGG ACCCCCAGGC CCTCCAGGCT CAACTGGTCT AAGAGGGTTC 
ATTGGTTTTC CAGGACTTCC AGGTGACCAG GGTGAGCCAG GTTCTCCAGG TCCCCCTGGA 
TTTTCAGGAA TTGATGGAGC AAGAGGACCT AAAGGAAACA AAGGTGACCC TGCCAGTCAC 
TTTGGTCCAC CTGGTCCAAA GGGTGAGCCA GGTAGCCCTG GATGTCCAGG GCATTTTGGA 
GCATCTGGAG AGCAGGGCTT GCCTGGTATT CAAGGGCCCA GAGGATCACC TGGAAGGCCA 
GGGCCACCTG GCTCCTCTGG ACCACCAGGG TGCCCAGGTG ATCACGGGAT GCCTGGGCTG 
AGGGGACAGC CAGGAGAAAT GGGAGACCCT GGGCCAAGAG GCCTCCAGGG GGATCCAGGG 
ATACCAGGTC CTCCGGGAAT AAAAGGTCCC TCCGGATCAC CTGGCCTGAA CGGCTTGCAT 
GGATTGAAAG GTCAGAAAGG AACTAAAGGT GCTTCAGGTT TGCATGATGT GGGGCCACCT 
GGTCCAGTGG GAATACCTGG GCTAAAAGGG GAGAGAGGAG ACCCTGGGAG CCCAGGAATC 
TCTCCTCCAG GTCCTCGTGG AGAAAAAGGT CCCCCAGGAC CCCCAGGGAG TTCAGGACCA 
CCTGGTCCTG CAGGTGCCAC AGGAAGAGCT CCTAAGGACA TTCCTGACCC GGGTCCACCT 
GGAGATCAGG GACCTCCTGG TCCTGATGGC CCAAGAGGAG CACCTGGGCC TCCAGGCCTC 
CCTGGGAGTG TTGACCTTCT GAGAGGGGAG CCAGGTGACT GTGGTCTACC AGGGCCACCA 
GGTCCCCCTG GCCCACCAGG CCCTCCAGGA TACAAAGGCT TTCCAGGATG TGATGGAAAA 
GATGGCCAGA AAGGACCAAT GGGATTCCCG GGACCGCAGG GACCACATGG ATTTCCTGGG 
CCACCTGGAG AGAAGGGTTT ACCTGGACCT CCAGGGAGAA AAGGGCCCAC TGGTCTTCCA 
GGTCCCAGAG GTGAACCGGG GCCACCTGCA GATGTGGATG ACTGTCCCCG AATCCCAGGC 
CTTCCTGGGG CGCCAGGCAT GAGAGGACCA GAAGGAGCCA TGGGGCTCTC TGGAATGAGA 
GGCCCCCCAG GACCAGGGTG CAAAGGAGAG CCTGGGCTGG ATGGCAGGAG GGGTGTGGAT 
GGCGTCCCTG GGTCTCCTGG GCCTCCCGGA CGTAAAGGTG ACACAGGAGA AGACGGCTAC 
CCTGGAGGAC CAGGTCTGGC AGGGTCTTGC CTTCCCGTGT TTAGCACGCT GCCCTTTGCC 
TACTGCAACA TCCACCAGGT GTGCCACTAT GCCCAGAGAA ACGACAGATC CTACTGGCTG 
GCCAGCGCTG CGCCCCTCCC CATGATGCCA CTCTCTGAAG AGGCGATCCG CCCCTATGTC 
AGCCGCTGTG CGGTATGCGA GGCCCCGGCC CAGGCGGTGG CGGTGCACAG CCAGGACAAG 
TCCATCCCCC CATGTCCGCA GACCTGGAGG AGCCTCTGGA TCGGGTATTC ATTCCTGATG 
CACACAGGAG CTGGGGACCA AGGAGGAGGG CAGGCCCTTA TGTCACCTGG CAGCTGCCTG 
GAAGATTTCA GAGCAGCACC ATTCCTTGAA TGCCAGGGCC GGCAGGGAAC TTGCCACTTT 
TTTGCAAATA AGTATAGCTT CTGGCTCACA ACGGTGAAAG CAGACTTGCA GTTTTCCTCT 
GCTCCAGCAC CAGACACCTT AAAAGAAAGC CAGGCCCAAC GCCAGAAAAT CAGCCGGTGC 
CAGGTCTGCG TGAAGTATAG CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009442743.2
CDS	424..5307
Translation	MWSLHIVLMRCSFRLTKSLATDPWSLTLILFSVQYVYGSGKKYVGPCGGRDCSVCHCVPEKGSRGPPGPPGPQGPIGPLGAPGPIGLSGEKGMRGDHGPPGAAGDKGDKGSTGVPGFPGLDGIPGHPGPPGPRGKPGMSGHNGSRGDPGFPGGRGALGPGGPLGHPGEKGEKGNSVFILGAVKGIQGDRGDPGLPGLPGSWGAGGPAGPTGYPGEPGLVGPPGQPGRPGLKGNPGVGVKGQMGDPGEVGQQGSPGPTLLVEPPDFRLYKGEKGIKGIPGMVGLPGPPGRKGESDIGAKGEKGIPGFPGPRGDPGSYGSPGFPGLKGELGLVGDPGLFGLIGPKGDPGNRGHPGPPGVLVTPPLPLKGPPGDPGFPGRYGETGDVGPPGPPGLLGRPGEACAGMIGPPGPQGFPGLPGLPGEAGIPGRPDSAPGKPGKPGSPGLPGAPGLQGLPGSSVIYCSVGNPGPQGIKGKVGPPGGRGSKGEKGNEGLCACEPGPMGPPGPPGLPGRQGSKGDLGLPGWLGTKGDPGPPGAEGPPGLPGKHGASGPPGNKGAKGDMVVSRVKGHKGERGPDGPPGFPGQPGSHGRDGHAGEKGDPGPPGDHEDATPGGKGFPGPLGPPGKAGPLGPPGLGFPGPPGQRGHPGVPGHPGVRGPDGLKGQKGDTISCNVTYPGRHGPPGFDGPPGPKGFPGPQGAPGLSGSDGHKGRPGTPGTAEIPGPPGFRGDMGDPGFGGEKGSSPAGPPGPPGSPGVNGQKGIPGDPAFGHLGPPGKRGLSGVPGIKGPRGDPGCPGAEGPAGIPGFPGLKGPKGREGHAGFPGVPGPPGHSCERGAPGIPGQPGLPGYPGSPGAPGGKGQLGDVGPPGPAGMKGLPGLPGWPGAHGPPGLPGIPGPFGDDGLPGPPGPKGPQGLPGFPGFPGERGKPGAEGCPGTKGEPGEKGMSGLPGDRGLRGAKGAIGPPGDEGEMAIISQKGTPGEPGPPGDDGFPGERGDKGTPGMQGRRGEPGRYGPPGFQRGEPGEKGQPGHPGPPGPPGSTGLRGFIGFPGLPGDQGEPGSPGPPGFSGIDGARGPKGNKGDPASHFGPPGPKGEPGSPGCPGHFGASGEQGLPGIQGPRGSPGRPGPPGSSGPPGCPGDHGMPGLRGQPGEMGDPGPRGLQGDPGIPGPPGIKGPSGSPGLNGLHGLKGQKGTKGASGLHDVGPPGPVGIPGLKGERGDPGSPGISPPGPRGEKGPPGPPGSSGPPGPAGATGRAPKDIPDPGPPGDQGPPGPDGPRGAPGPPGLPGSVDLLRGEPGDCGLPGPPGPPGPPGPPGYKGFPGCDGKDGQKGPMGFPGPQGPHGFPGPPGEKGLPGPPGRKGPTGLPGPRGEPGPPADVDDCPRIPGLPGAPGMRGPEGAMGLSGMRGPPGPGCKGEPGLDGRRGVDGVPGSPGPPGRKGDTGEDGYPGGPGLAGSCLPVFSTLPFAYCNIHQVCHYAQRNDRSYWLASAAPLPMMPLSEEAIRPYVSRCAVCEAPAQAVAVHSQDKSIPPCPQTWRSLWIGYSFLMHTGAGDQGGGQALMSPGSCLEDFRAAPFLECQGRQGTCHFFANKYSFWLTTVKADLQFSSAPAPDTLKESQAQRQKISRCQVCVKYS

Target ORF information:

RefSeq Version	XM_009444468.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes collagen type IV alpha 4 (COL4A4), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009444468.2

ORF Insert Sequence:

ATGTGGTCTC TGCACATAGT ACTAATGAGG TGCTCCTTCA GATTGACCAA GTCCTTGGCC 
ACAGATCCCT GGTCACTTAC ACTCATTCTC TTTTCTGTAC AATATGTATA TGGGAGTGGA 
AAGAAATACG TTGGTCCTTG TGGAGGAAGA GATTGCTCTG TTTGCCACTG TGTTCCTGAA 
AAGGGGTCTC GGGGTCCACC AGGACCACCA GGGCCACAGG GTCCAATTGG ACCCCTGGGA 
GCCCCAGGAC CCATTGGGCT TTCAGGAGAG AAAGGAATGA GAGGGGACCA CGGCCCTCCT 
GGAGCAGCAG GGGACAAAGG AGATAAGGGT TCAACTGGTG TTCCTGGATT TCCAGGTTTA 
GATGGCATAC CTGGGCACCC AGGGCCTCCT GGACCCAGAG GCAAACCTGG TATGAGTGGC 
CACAATGGCT CAAGAGGTGA CCCAGGGTTT CCAGGAGGAA GAGGAGCTCT TGGCCCAGGA 
GGCCCCCTAG GCCATCCTGG GGAAAAGGGA GAAAAAGGAA ATTCAGTGTT CATTTTAGGT 
GCCGTTAAAG GTATTCAGGG AGACAGAGGG GACCCAGGAC TGCCTGGCTT ACCAGGATCT 
TGGGGGGCAG GAGGACCGGC GGGTCCCACA GGATATCCTG GAGAGCCAGG GTTAGTGGGA 
CCTCCGGGCC AACCAGGGCG TCCAGGTTTG AAGGGAAATC CCGGTGTGGG AGTAAAGGGG 
CAAATGGGAG ACCCGGGTGA GGTTGGTCAG CAAGGTTCTC CTGGACCCAC CCTGTTGGTG 
GAGCCACCTG ACTTTCGTCT CTATAAAGGA GAAAAGGGTA TAAAAGGAAT TCCTGGAATG 
GTTGGACTGC CAGGACCACC AGGACGCAAG GGAGAATCTG ATATTGGGGC AAAAGGAGAA 
AAAGGTATTC CTGGATTTCC AGGGCCTCGG GGGGATCCTG GTTCCTATGG ATCTCCAGGT 
TTTCCAGGAT TAAAGGGAGA ACTAGGACTG GTTGGAGATC CTGGGCTATT TGGATTAATT 
GGCCCAAAGG GGGATCCTGG AAATCGAGGG CACCCAGGAC CACCAGGTGT TTTGGTGACT 
CCACCTCTTC CACTCAAAGG CCCACCAGGG GACCCAGGGT TCCCTGGCCG CTATGGAGAA 
ACAGGGGATG TTGGACCACC TGGTCCCCCA GGTCTCTTGG GCAGACCAGG GGAAGCCTGT 
GCAGGCATGA TAGGACCCCC TGGGCCACAA GGATTTCCTG GTCTTCCTGG GCTTCCAGGA 
GAAGCTGGTA TTCCTGGGAG ACCTGATTCT GCTCCAGGAA AACCAGGGAA GCCAGGATCA 
CCTGGCTTGC CTGGAGCACC AGGCCTGCAG GGCCTCCCAG GATCAAGTGT GATATACTGT 
AGTGTTGGGA ATCCTGGACC ACAAGGAATA AAAGGCAAAG TGGGTCCCCC AGGAGGAAGA 
GGCTCAAAAG GAGAAAAAGG AAATGAAGGA CTCTGTGCCT GTGAGCCTGG ACCCATGGGC 
CCCCCTGGCC CTCCAGGACT TCCTGGGAGG CAGGGGAGTA AGGGAGACTT GGGGCTCCCT 
GGCTGGCTTG GAACAAAAGG TGACCCAGGA CCTCCTGGTG CTGAAGGACC TCCAGGGCTA 
CCAGGAAAGC ATGGTGCCTC TGGACCACCT GGCAACAAAG GGGCGAAGGG TGACATGGTT 
GTATCAAGAG TTAAAGGGCA CAAAGGAGAA AGAGGTCCTG ATGGGCCCCC AGGATTTCCA 
GGGCAGCCAG GATCACATGG TCGGGATGGA CATGCTGGAG AAAAAGGGGA TCCAGGACCC 
CCAGGGGATC ATGAAGATGC GACCCCAGGT GGTAAAGGAT TTCCTGGACC TCTGGGCCCC 
CCGGGCAAAG CAGGACCTTT GGGGCCCCCA GGACTGGGAT TTCCTGGTCC ACCAGGACAG 
CGAGGCCACC CAGGAGTTCC AGGCCACCCA GGTGTGAGGG GCCCTGATGG CTTGAAGGGT 
CAGAAAGGTG ACACAATTTC TTGCAACGTA ACCTACCCTG GGAGGCACGG CCCTCCAGGT 
TTTGATGGAC CTCCAGGTCC GAAGGGATTT CCAGGTCCCC AAGGTGCCCC TGGGCTGAGT 
GGTTCAGATG GGCATAAAGG CAGACCTGGC ACACCAGGAA CAGCGGAAAT ACCAGGTCCA 
CCTGGTTTTC GTGGTGACAT GGGAGATCCG GGTTTTGGAG GTGAAAAGGG GTCCTCCCCT 
GCTGGGCCCC CAGGCCCTCC CGGCTCACCA GGAGTGAATG GTCAGAAAGG AATCCCGGGA 
GACCCTGCAT TTGGTCACCT GGGACCCCCG GGAAAGAGGG GTCTTTCAGG AGTGCCAGGG 
ATAAAAGGAC CCAGAGGTGA TCCGGGATGT CCAGGGGCTG AAGGGCCAGC TGGCATTCCT 
GGATTCCCAG GTCTCAAAGG TCCCAAAGGC AGAGAGGGAC ATGCTGGGTT TCCAGGTGTC 
CCAGGTCCAC CTGGCCATTC CTGTGAAAGA GGTGCTCCAG GGATACCAGG GCAACCGGGA 
CTCCCTGGGT ATCCAGGTAG CCCAGGTGCT CCAGGTGGGA AAGGACAGCT GGGAGATGTG 
GGGCCTCCCG GGCCAGCTGG AATGAAAGGC CTCCCTGGAC TCCCAGGATG GCCTGGGGCA 
CATGGTCCCC CAGGCCTCCC AGGAATCCCA GGTCCCTTTG GAGATGATGG GCTACCTGGT 
CCTCCAGGTC CAAAGGGACC CCAGGGGCTG CCTGGTTTCC CAGGTTTTCC CGGAGAAAGA 
GGAAAGCCTG GTGCAGAGGG ATGTCCTGGC ACAAAGGGAG AACCTGGAGA GAAGGGCATG 
TCTGGCCTTC CCGGAGACCG GGGACTGAGA GGGGCCAAAG GAGCCATAGG ACCTCCCGGA 
GATGAAGGAG AAATGGCTAT CATTTCCCAA AAGGGAACAC CTGGGGAACC TGGACCTCCT 
GGAGATGATG GATTCCCAGG AGAAAGAGGT GATAAAGGAA CTCCCGGGAT GCAAGGGAGA 
AGAGGAGAGC CGGGAAGATA CGGACCACCT GGATTTCAGA GAGGGGAACC TGGTGAGAAA 
GGTCAGCCAG GGCATCCTGG ACCCCCAGGC CCTCCAGGCT CAACTGGTCT AAGAGGGTTC 
ATTGGTTTTC CAGGACTTCC AGGTGACCAG GGTGAGCCAG GTTCTCCAGG TCCCCCTGGA 
TTTTCAGGAA TTGATGGAGC AAGAGGACCT AAAGGAAACA AAGGTGACCC TGCCAGTCAC 
TTTGGTCCAC CTGGTCCAAA GGGTGAGCCA GGTAGCCCTG GATGTCCAGG GCATTTTGGA 
GCATCTGGAG AGCAGGGCTT GCCTGGTATT CAAGGGCCCA GAGGATCACC TGGAAGGCCA 
GGGCCACCTG GCTCCTCTGG ACCACCAGGG TGCCCAGGTG ATCACGGGAT GCCTGGGCTG 
AGGGGACAGC CAGGAGAAAT GGGAGACCCT GGGCCAAGAG GCCTCCAGGG GGATCCAGGG 
ATACCAGGTC CTCCGGGAAT AAAAGGTCCC TCCGGATCAC CTGGCCTGAA CGGCTTGCAT 
GGATTGAAAG GTCAGAAAGG AACTAAAGGT GCTTCAGGTT TGCATGATGT GGGGCCACCT 
GGTCCAGTGG GAATACCTGG GCTAAAAGGG GAGAGAGGAG ACCCTGGGAG CCCAGGAATC 
TCTCCTCCAG GTCCTCGTGG AGAAAAAGGT CCCCCAGGAC CCCCAGGGAG TTCAGGACCA 
CCTGGTCCTG CAGGTGCCAC AGGAAGAGCT CCTAAGGACA TTCCTGACCC GGGTCCACCT 
GGAGATCAGG GACCTCCTGG TCCTGATGGC CCAAGAGGAG CACCTGGGCC TCCAGGCCTC 
CCTGGGAGTG TTGACCTTCT GAGAGGGGAG CCAGGTGACT GTGGTCTACC AGGGCCACCA 
GGTCCCCCTG GCCCACCAGG CCCTCCAGGA TACAAAGGCT TTCCAGGATG TGATGGAAAA 
GATGGCCAGA AAGGACCAAT GGGATTCCCG GGACCGCAGG GACCACATGG ATTTCCTGGG 
CCACCTGGAG AGAAGGGTTT ACCTGGACCT CCAGGGAGAA AAGGGCCCAC TGGTCTTCCA 
GGTCCCAGAG GTGAACCGGG GCCACCTGCA GATGTGGATG ACTGTCCCCG AATCCCAGGC 
CTTCCTGGGG CGCCAGGCAT GAGAGGACCA GAAGGAGCCA TGGGGCTCTC TGGAATGAGA 
GGCCCCCCAG GACCAGGGTG CAAAGGAGAG CCTGGGCTGG ATGGCAGGAG GGGTGTGGAT 
GGCGTCCCTG GGTCTCCTGG GCCTCCCGGA CGTAAAGGTG ACACAGGAGA AGACGGCTAC 
CCTGGAGGAC CAGGTCTGGC AGGGTCTTGC CTTCCCGTGT TTAGCACGCT GCCCTTTGCC 
TACTGCAACA TCCACCAGGT GTGCCACTAT GCCCAGAGAA ACGACAGATC CTACTGGCTG 
GCCAGCGCTG CGCCCCTCCC CATGATGCCA CTCTCTGAAG AGGCGATCCG CCCCTATGTC 
AGCCGCTGTG CGGTATGCGA GGCCCCGGCC CAGGCGGTGG CGGTGCACAG CCAGGACAAG 
TCCATCCCCC CATGTCCGCA GACCTGGAGG AGCCTCTGGA TCGGGTATTC ATTCCTGATG 
CACACAGGAG CTGGGGACCA AGGAGGAGGG CAGGCCCTTA TGTCACCTGG CAGCTGCCTG 
GAAGATTTCA GAGCAGCACC ATTCCTTGAA TGCCAGGGCC GGCAGGGAAC TTGCCACTTT 
TTTGCAAATA AGTATAGCTT CTGGCTCACA ACGGTGAAAG CAGACTTGCA GTTTTCCTCT 
GCTCCAGCAC CAGACACCTT AAAAGAAAGC CAGGCCCAAC GCCAGAAAAT CAGCCGGTGC 
CAGGTCTGCG TGAAGTATAG CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL4A4 ( XM_016950593.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016806082.1 Pan troglodytes collagen type IV alpha 4 (COL4A4), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OPc80768
Clone ID Related Accession (Same CDS sequence)	XM_016950593.1 , XM_009444469.2 , XM_009444470.2
Accession Version	XM_016950593.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4827bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	collagen alpha-4(IV) chain isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006470.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGTCTC TGCACATAGT ACTAATGAGG TGCTCCTTCA GATTGACCAA GTCCTTGGCC 
ACAGATCCCT GGTCACTTAC ACTCATTCTC TTTTCTGTAC AATATGTATA TGGGAGTGGA 
AAGAAATACG TTGGTCCTTG TGGAGGAAGA GATTGCTCTG TTTGCCACTG TGTTCCTGAA 
AAGGGGTCTC GGGGTCCACC AGGACCACCA GGGCCACAGG GTCCAATTGG ACCCCTGGGA 
GCCCCAGGAC CCATTGGGCT TTCAGGAGAG AAAGGAATGA GAGGGGACCA CGGCCCTCCT 
GGAGCAGCAG GGGACAAAGG AGATAAGGGT TCAACTGGTG TTCCTGGATT TCCAGGTTTA 
GATGGCATAC CTGGGCACCC AGGGCCTCCT GGACCCAGAG GCAAACCTGG TATGAGTGGC 
CACAATGGCT CAAGAGGTGA CCCAGGGTTT CCAGGAGGAA GAGGAGCTCT TGGCCCAGGA 
GGCCCCCTAG GCCATCCTGG GGAAAAGGGA GAAAAAGGAA ATTCAGTGTT CATTTTAGGT 
GCCGTTAAAG GTATTCAGGG AGACAGAGGG GACCCAGGAC TGCCTGGCTT ACCAGGATCT 
TGGGGGGCAG GAGGACCGGC GGGTCCCACA GGATATCCTG GAGAGCCAGG GTTAGTGGGA 
CCTCCGGGCC AACCAGGGCG TCCAGGTTTG AAGGGAAATC CCGGTGTGGG AGTAAAGGGG 
CAAATGGGAG ACCCGGGTGA GGTTGGTCAG CAAGGTTCTC CTGGACCCAC CCTGTTGGTG 
GAGCCACCTG ACTTTCGTCT CTATAAAGGA GAAAAGGGTA TAAAAGGAAT TCCTGGAATG 
GTTGGACTGC CAGGACCACC AGGACGCAAG GGAGAATCTG ATATTGGGGC AAAAGGAGAA 
AAAGGTATTC CTGGATTTCC AGGGCCTCGG GGGGATCCTG GTTCCTATGG ATCTCCAGGT 
TTTCCAGGAT TAAAGGGAGA ACTAGGACTG GTTGGAGATC CTGGGCTATT TGGATTAATT 
GGCCCAAAGG GGGATCCTGG AAATCGAGGG CACCCAGGAC CACCAGGTGT TTTGGTGACT 
CCACCTCTTC CACTCAAAGG CCCACCAGGG GACCCAGGGT TCCCTGGCCG CTATGGAGAA 
ACAGGGGATG TTGGACCACC TGGTCCCCCA GGTCTCTTGG GCAGACCAGG GGAAGCCTGT 
GCAGGCATGA TAGGACCCCC TGGGCCACAA GGATTTCCTG GTCTTCCTGG GCTTCCAGGA 
GAAGCTGGTA TTCCTGGGAG ACCTGATTCT GCTCCAGGAA AACCAGGGAA GCCAGGATCA 
CCTGGCTTGC CTGGAGCACC AGGCCTGCAG GGCCTCCCAG GATCAAGTGT GATATACTGT 
AGTGTTGGGA ATCCTGGACC ACAAGGAATA AAAGGCAAAG TGGGTCCCCC AGGAGGAAGA 
GGCTCAAAAG GAGAAAAAGG AAATGAAGGA CTCTGTGCCT GTGAGCCTGG ACCCATGGGC 
CCCCCTGGCC CTCCAGGACT TCCTGGGAGG CAGGGGAGTA AGGGAGACTT GGGGCTCCCT 
GGCTGGCTTG GAACAAAAGG TGACCCAGGA CCTCCTGGTG CTGAAGGACC TCCAGGGCTA 
CCAGGAAAGC ATGGTGCCTC TGGACCACCT GGCAACAAAG GGGCGAAGGG TGACATGGTT 
GTATCAAGAG TTAAAGGGCA CAAAGGAGAA AGAGGTCCTG ATGGGCCCCC AGGATTTCCA 
GGGCAGCCAG GATCACATGG TCGGGATGGA CATGCTGGAG AAAAAGGGGA TCCAGGACCC 
CCAGGGGATC ATGAAGATGC GACCCCAGGT GGTAAAGGAT TTCCTGGACC TCTGGGCCCC 
CCGGGCAAAG CAGGACCTTT GGGGCCCCCA GGACTGGGAT TTCCTGGTCC ACCAGGACAG 
CGAGGCCACC CAGGAGTTCC AGGCCACCCA GGTGTGAGGG GCCCTGATGG CTTGAAGGGT 
CAGAAAGGTG ACACAATTTC TTGCAACGTA ACCTACCCTG GGAGGCACGG CCCTCCAGGT 
TTTGATGGAC CTCCAGGTCC GAAGGGATTT CCAGGTCCCC AAGGTGCCCC TGGGCTGAGT 
GGTTCAGATG GGCATAAAGG CAGACCTGGC ACACCAGGAA CAGCGGAAAT ACCAGGTCCA 
CCTGGTTTTC GTGGTGACAT GGGAGATCCG GGTTTTGGAG GTGAAAAGGG GTCCTCCCCT 
GCTGGGCCCC CAGGCCCTCC CGGCTCACCA GGAGTGAATG GTCAGAAAGG AATCCCGGGA 
GACCCTGCAT TTGGTCACCT GGGACCCCCG GGAAAGAGGG GTCTTTCAGG AGTGCCAGGG 
ATAAAAGGAC CCAGAGGTGA TCCGGGATGT CCAGGGGCTG AAGGGCCAGC TGGCATTCCT 
GGATTCCCAG GTCTCAAAGG TCCCAAAGGC AGAGAGGGAC ATGCTGGGTT TCCAGGTGTC 
CCAGGTCCAC CTGGCCATTC CTGTGAAAGA GGTGCTCCAG GGATACCAGG GCAACCGGGA 
CTCCCTGGGT ATCCAGGTAG CCCAGGTGCT CCAGGTGGGA AAGGACAGCT GGGAGATGTG 
GGGCCTCCCG GGCCAGCTGG AATGAAAGGC CTCCCTGGAC TCCCAGGATG GCCTGGGGCA 
CATGGTCCCC CAGGCCTCCC AGGAATCCCA GGTCCCTTTG GAGATGATGG GCTACCTGGT 
CCTCCAGGTC CAAAGGGACC CCAGGGGCTG CCTGGTTTCC CAGGTTTTCC CGGAGAAAGA 
GGAAAGCCTG GTGCAGAGGG ATGTCCTGGC ACAAAGGGAG AACCTGGAGA GAAGGGCATG 
TCTGGCCTTC CCGGAGACCG GGGACTGAGA GGGGCCAAAG GAGCCATAGG ACCTCCCGGA 
GATGAAGGAG AAATGGCTAT CATTTCCCAA AAGGGAACAC CTGGGGAACC TGGACCTCCT 
GGAGATGATG GATTCCCAGG AGAAAGAGGT GATAAAGGAA CTCCCGGGAT GCAAGGGAGA 
AGAGGAGAGC CGGGAAGATA CGGACCACCT GGATTTCAGA GAGGGGAACC TGGTGAGAAA 
GGTCAGCCAG GGCATCCTGG ACCCCCAGGC CCTCCAGGCT CAACTGGTCT AAGAGGGTTC 
ATTGGTTTTC CAGGACTTCC AGGTGACCAG GGTGAGCCAG GTTCTCCAGG TCCCCCTGGA 
TTTTCAGGAA TTGATGGAGC AAGAGGACCT AAAGGAAACA AAGGTGACCC TGCCAGTCAC 
TTTGGTCCAC CTGGTCCAAA GGGTGAGCCA GGTAGCCCTG GATGTCCAGG GCATTTTGGA 
GCATCTGGAG AGCAGGGCTT GCCTGGTATT CAAGGGCCCA GAGGATCACC TGGAAGGCCA 
GGGCCACCTG GCTCCTCTGG ACCACCAGGG TGCCCAGGTG ATCACGGGAT GCCTGGGCTG 
AGGGGACAGC CAGGAGAAAT GGGAGACCCT GGGCCAAGAG GCCTCCAGGG GGATCCAGGG 
ATACCAGGTC CTCCGGGAAT AAAAGGTCCC TCCGGATCAC CTGGCCTGAA CGGCTTGCAT 
GGATTGAAAG GTCAGAAAGG AACTAAAGGT GCTTCAGGTT TGCATGATGT GGGGCCACCT 
GGTCCAGTGG GAATACCTGG GCTAAAAGGG GAGAGAGGAG ACCCTGGGAG CCCAGGAATC 
TCTCCTCCAG GTCCTCGTGG AGAAAAAGGT CCCCCAGGAC CCCCAGGGAG TTCAGGACCA 
CCTGGTCCTG CAGGTGCCAC AGGAAGAGCT CCTAAGGACA TTCCTGACCC GGGTCCACCT 
GGAGATCAGG GACCTCCTGG TCCTGATGGC CCAAGAGGAG CACCTGGGCC TCCAGGCCTC 
CCTGGGAGTG TTGACCTTCT GAGAGGGGAG CCAGGTGACT GTGGTCTACC AGGGCCACCA 
GGTCCCCCTG GCCCACCAGG CCCTCCAGGA TACAAAGGCT TTCCAGGATG TGATGGAAAA 
GATGGCCAGA AAGGACCAAT GGGATTCCCG GGACCGCAGG GACCACATGG ATTTCCTGGG 
CCACCTGGAG AGAAGGGTTT ACCTGGACCT CCAGGGAGAA AAGGGCCCAC TGGTCTTCCA 
GGTCCCAGAG GTGAACCGGG GCCACCTGCA GATGTGGATG ACTGTCCCCG AATCCCAGGC 
CTTCCTGGGG CGCCAGGCAT GAGAGGACCA GAAGGAGCCA TGGGGCTCTC TGGAATGAGA 
GGCCCCCCAG GACCAGGGTG CAAAGGAGAG CCTGGGCTGG ATGGCAGGAG GGGTGTGGAT 
GGCGTCCCTG GGTCTCCTGG GCCTCCCGGA CGTAAAGGTG ACACAGGAGA AGACGGCTAC 
CCTGGAGGAC CAGGGCCTCC TGGTCCCATT GGGGATCCTG GGCCCAAAGG GTTTGGCCCT 
GGATACCTCG GTGGCTTCCT CCTGGTTCTC CACAGTCAGA CGGACCAGGA GCCCACCTGC 
CCCCTGGGCA TGCCCAGGCT CTGGACTGGG TATAGTCTGT TATACCTGGA AGGGCAAGAG 
AAAGCTCACA ATCAAGACCT TGGTCTGGCA GGGTCTTGCC TTCCCGTGTT TAGCACGCTG 
CCCTTTGCCT ACTGCAACAT CCACCAGGTG TGCCACTATG CCCAGAGAAA CGACAGATCC 
TACTGGCTGG CCAGCGCTGC GCCCCTCCCC ATGATGCCAC TCTCTGAAGA GGCGATCCGC 
CCCTATGTCA GCCGCTGTGC GGTATGCGAG GCCCCGGCCC AGGCGGTGGC GGTGCACAGC 
CAGGACAAGT CCATCCCCCC ATGTCCGCAG ACCTGGAGGA GCCTCTGGAT CGGGTATTCA 
TTCCTGATGC TCATGGGTGT GTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016806082.1
CDS	424..5250
Translation	MWSLHIVLMRCSFRLTKSLATDPWSLTLILFSVQYVYGSGKKYVGPCGGRDCSVCHCVPEKGSRGPPGPPGPQGPIGPLGAPGPIGLSGEKGMRGDHGPPGAAGDKGDKGSTGVPGFPGLDGIPGHPGPPGPRGKPGMSGHNGSRGDPGFPGGRGALGPGGPLGHPGEKGEKGNSVFILGAVKGIQGDRGDPGLPGLPGSWGAGGPAGPTGYPGEPGLVGPPGQPGRPGLKGNPGVGVKGQMGDPGEVGQQGSPGPTLLVEPPDFRLYKGEKGIKGIPGMVGLPGPPGRKGESDIGAKGEKGIPGFPGPRGDPGSYGSPGFPGLKGELGLVGDPGLFGLIGPKGDPGNRGHPGPPGVLVTPPLPLKGPPGDPGFPGRYGETGDVGPPGPPGLLGRPGEACAGMIGPPGPQGFPGLPGLPGEAGIPGRPDSAPGKPGKPGSPGLPGAPGLQGLPGSSVIYCSVGNPGPQGIKGKVGPPGGRGSKGEKGNEGLCACEPGPMGPPGPPGLPGRQGSKGDLGLPGWLGTKGDPGPPGAEGPPGLPGKHGASGPPGNKGAKGDMVVSRVKGHKGERGPDGPPGFPGQPGSHGRDGHAGEKGDPGPPGDHEDATPGGKGFPGPLGPPGKAGPLGPPGLGFPGPPGQRGHPGVPGHPGVRGPDGLKGQKGDTISCNVTYPGRHGPPGFDGPPGPKGFPGPQGAPGLSGSDGHKGRPGTPGTAEIPGPPGFRGDMGDPGFGGEKGSSPAGPPGPPGSPGVNGQKGIPGDPAFGHLGPPGKRGLSGVPGIKGPRGDPGCPGAEGPAGIPGFPGLKGPKGREGHAGFPGVPGPPGHSCERGAPGIPGQPGLPGYPGSPGAPGGKGQLGDVGPPGPAGMKGLPGLPGWPGAHGPPGLPGIPGPFGDDGLPGPPGPKGPQGLPGFPGFPGERGKPGAEGCPGTKGEPGEKGMSGLPGDRGLRGAKGAIGPPGDEGEMAIISQKGTPGEPGPPGDDGFPGERGDKGTPGMQGRRGEPGRYGPPGFQRGEPGEKGQPGHPGPPGPPGSTGLRGFIGFPGLPGDQGEPGSPGPPGFSGIDGARGPKGNKGDPASHFGPPGPKGEPGSPGCPGHFGASGEQGLPGIQGPRGSPGRPGPPGSSGPPGCPGDHGMPGLRGQPGEMGDPGPRGLQGDPGIPGPPGIKGPSGSPGLNGLHGLKGQKGTKGASGLHDVGPPGPVGIPGLKGERGDPGSPGISPPGPRGEKGPPGPPGSSGPPGPAGATGRAPKDIPDPGPPGDQGPPGPDGPRGAPGPPGLPGSVDLLRGEPGDCGLPGPPGPPGPPGPPGYKGFPGCDGKDGQKGPMGFPGPQGPHGFPGPPGEKGLPGPPGRKGPTGLPGPRGEPGPPADVDDCPRIPGLPGAPGMRGPEGAMGLSGMRGPPGPGCKGEPGLDGRRGVDGVPGSPGPPGRKGDTGEDGYPGGPGPPGPIGDPGPKGFGPGYLGGFLLVLHSQTDQEPTCPLGMPRLWTGYSLLYLEGQEKAHNQDLGLAGSCLPVFSTLPFAYCNIHQVCHYAQRNDRSYWLASAAPLPMMPLSEEAIRPYVSRCAVCEAPAQAVAVHSQDKSIPPCPQTWRSLWIGYSFLMLMGVF

Target ORF information:

RefSeq Version	XM_016950593.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes collagen type IV alpha 4 (COL4A4), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016950593.1

ORF Insert Sequence:

ATGTGGTCTC TGCACATAGT ACTAATGAGG TGCTCCTTCA GATTGACCAA GTCCTTGGCC 
ACAGATCCCT GGTCACTTAC ACTCATTCTC TTTTCTGTAC AATATGTATA TGGGAGTGGA 
AAGAAATACG TTGGTCCTTG TGGAGGAAGA GATTGCTCTG TTTGCCACTG TGTTCCTGAA 
AAGGGGTCTC GGGGTCCACC AGGACCACCA GGGCCACAGG GTCCAATTGG ACCCCTGGGA 
GCCCCAGGAC CCATTGGGCT TTCAGGAGAG AAAGGAATGA GAGGGGACCA CGGCCCTCCT 
GGAGCAGCAG GGGACAAAGG AGATAAGGGT TCAACTGGTG TTCCTGGATT TCCAGGTTTA 
GATGGCATAC CTGGGCACCC AGGGCCTCCT GGACCCAGAG GCAAACCTGG TATGAGTGGC 
CACAATGGCT CAAGAGGTGA CCCAGGGTTT CCAGGAGGAA GAGGAGCTCT TGGCCCAGGA 
GGCCCCCTAG GCCATCCTGG GGAAAAGGGA GAAAAAGGAA ATTCAGTGTT CATTTTAGGT 
GCCGTTAAAG GTATTCAGGG AGACAGAGGG GACCCAGGAC TGCCTGGCTT ACCAGGATCT 
TGGGGGGCAG GAGGACCGGC GGGTCCCACA GGATATCCTG GAGAGCCAGG GTTAGTGGGA 
CCTCCGGGCC AACCAGGGCG TCCAGGTTTG AAGGGAAATC CCGGTGTGGG AGTAAAGGGG 
CAAATGGGAG ACCCGGGTGA GGTTGGTCAG CAAGGTTCTC CTGGACCCAC CCTGTTGGTG 
GAGCCACCTG ACTTTCGTCT CTATAAAGGA GAAAAGGGTA TAAAAGGAAT TCCTGGAATG 
GTTGGACTGC CAGGACCACC AGGACGCAAG GGAGAATCTG ATATTGGGGC AAAAGGAGAA 
AAAGGTATTC CTGGATTTCC AGGGCCTCGG GGGGATCCTG GTTCCTATGG ATCTCCAGGT 
TTTCCAGGAT TAAAGGGAGA ACTAGGACTG GTTGGAGATC CTGGGCTATT TGGATTAATT 
GGCCCAAAGG GGGATCCTGG AAATCGAGGG CACCCAGGAC CACCAGGTGT TTTGGTGACT 
CCACCTCTTC CACTCAAAGG CCCACCAGGG GACCCAGGGT TCCCTGGCCG CTATGGAGAA 
ACAGGGGATG TTGGACCACC TGGTCCCCCA GGTCTCTTGG GCAGACCAGG GGAAGCCTGT 
GCAGGCATGA TAGGACCCCC TGGGCCACAA GGATTTCCTG GTCTTCCTGG GCTTCCAGGA 
GAAGCTGGTA TTCCTGGGAG ACCTGATTCT GCTCCAGGAA AACCAGGGAA GCCAGGATCA 
CCTGGCTTGC CTGGAGCACC AGGCCTGCAG GGCCTCCCAG GATCAAGTGT GATATACTGT 
AGTGTTGGGA ATCCTGGACC ACAAGGAATA AAAGGCAAAG TGGGTCCCCC AGGAGGAAGA 
GGCTCAAAAG GAGAAAAAGG AAATGAAGGA CTCTGTGCCT GTGAGCCTGG ACCCATGGGC 
CCCCCTGGCC CTCCAGGACT TCCTGGGAGG CAGGGGAGTA AGGGAGACTT GGGGCTCCCT 
GGCTGGCTTG GAACAAAAGG TGACCCAGGA CCTCCTGGTG CTGAAGGACC TCCAGGGCTA 
CCAGGAAAGC ATGGTGCCTC TGGACCACCT GGCAACAAAG GGGCGAAGGG TGACATGGTT 
GTATCAAGAG TTAAAGGGCA CAAAGGAGAA AGAGGTCCTG ATGGGCCCCC AGGATTTCCA 
GGGCAGCCAG GATCACATGG TCGGGATGGA CATGCTGGAG AAAAAGGGGA TCCAGGACCC 
CCAGGGGATC ATGAAGATGC GACCCCAGGT GGTAAAGGAT TTCCTGGACC TCTGGGCCCC 
CCGGGCAAAG CAGGACCTTT GGGGCCCCCA GGACTGGGAT TTCCTGGTCC ACCAGGACAG 
CGAGGCCACC CAGGAGTTCC AGGCCACCCA GGTGTGAGGG GCCCTGATGG CTTGAAGGGT 
CAGAAAGGTG ACACAATTTC TTGCAACGTA ACCTACCCTG GGAGGCACGG CCCTCCAGGT 
TTTGATGGAC CTCCAGGTCC GAAGGGATTT CCAGGTCCCC AAGGTGCCCC TGGGCTGAGT 
GGTTCAGATG GGCATAAAGG CAGACCTGGC ACACCAGGAA CAGCGGAAAT ACCAGGTCCA 
CCTGGTTTTC GTGGTGACAT GGGAGATCCG GGTTTTGGAG GTGAAAAGGG GTCCTCCCCT 
GCTGGGCCCC CAGGCCCTCC CGGCTCACCA GGAGTGAATG GTCAGAAAGG AATCCCGGGA 
GACCCTGCAT TTGGTCACCT GGGACCCCCG GGAAAGAGGG GTCTTTCAGG AGTGCCAGGG 
ATAAAAGGAC CCAGAGGTGA TCCGGGATGT CCAGGGGCTG AAGGGCCAGC TGGCATTCCT 
GGATTCCCAG GTCTCAAAGG TCCCAAAGGC AGAGAGGGAC ATGCTGGGTT TCCAGGTGTC 
CCAGGTCCAC CTGGCCATTC CTGTGAAAGA GGTGCTCCAG GGATACCAGG GCAACCGGGA 
CTCCCTGGGT ATCCAGGTAG CCCAGGTGCT CCAGGTGGGA AAGGACAGCT GGGAGATGTG 
GGGCCTCCCG GGCCAGCTGG AATGAAAGGC CTCCCTGGAC TCCCAGGATG GCCTGGGGCA 
CATGGTCCCC CAGGCCTCCC AGGAATCCCA GGTCCCTTTG GAGATGATGG GCTACCTGGT 
CCTCCAGGTC CAAAGGGACC CCAGGGGCTG CCTGGTTTCC CAGGTTTTCC CGGAGAAAGA 
GGAAAGCCTG GTGCAGAGGG ATGTCCTGGC ACAAAGGGAG AACCTGGAGA GAAGGGCATG 
TCTGGCCTTC CCGGAGACCG GGGACTGAGA GGGGCCAAAG GAGCCATAGG ACCTCCCGGA 
GATGAAGGAG AAATGGCTAT CATTTCCCAA AAGGGAACAC CTGGGGAACC TGGACCTCCT 
GGAGATGATG GATTCCCAGG AGAAAGAGGT GATAAAGGAA CTCCCGGGAT GCAAGGGAGA 
AGAGGAGAGC CGGGAAGATA CGGACCACCT GGATTTCAGA GAGGGGAACC TGGTGAGAAA 
GGTCAGCCAG GGCATCCTGG ACCCCCAGGC CCTCCAGGCT CAACTGGTCT AAGAGGGTTC 
ATTGGTTTTC CAGGACTTCC AGGTGACCAG GGTGAGCCAG GTTCTCCAGG TCCCCCTGGA 
TTTTCAGGAA TTGATGGAGC AAGAGGACCT AAAGGAAACA AAGGTGACCC TGCCAGTCAC 
TTTGGTCCAC CTGGTCCAAA GGGTGAGCCA GGTAGCCCTG GATGTCCAGG GCATTTTGGA 
GCATCTGGAG AGCAGGGCTT GCCTGGTATT CAAGGGCCCA GAGGATCACC TGGAAGGCCA 
GGGCCACCTG GCTCCTCTGG ACCACCAGGG TGCCCAGGTG ATCACGGGAT GCCTGGGCTG 
AGGGGACAGC CAGGAGAAAT GGGAGACCCT GGGCCAAGAG GCCTCCAGGG GGATCCAGGG 
ATACCAGGTC CTCCGGGAAT AAAAGGTCCC TCCGGATCAC CTGGCCTGAA CGGCTTGCAT 
GGATTGAAAG GTCAGAAAGG AACTAAAGGT GCTTCAGGTT TGCATGATGT GGGGCCACCT 
GGTCCAGTGG GAATACCTGG GCTAAAAGGG GAGAGAGGAG ACCCTGGGAG CCCAGGAATC 
TCTCCTCCAG GTCCTCGTGG AGAAAAAGGT CCCCCAGGAC CCCCAGGGAG TTCAGGACCA 
CCTGGTCCTG CAGGTGCCAC AGGAAGAGCT CCTAAGGACA TTCCTGACCC GGGTCCACCT 
GGAGATCAGG GACCTCCTGG TCCTGATGGC CCAAGAGGAG CACCTGGGCC TCCAGGCCTC 
CCTGGGAGTG TTGACCTTCT GAGAGGGGAG CCAGGTGACT GTGGTCTACC AGGGCCACCA 
GGTCCCCCTG GCCCACCAGG CCCTCCAGGA TACAAAGGCT TTCCAGGATG TGATGGAAAA 
GATGGCCAGA AAGGACCAAT GGGATTCCCG GGACCGCAGG GACCACATGG ATTTCCTGGG 
CCACCTGGAG AGAAGGGTTT ACCTGGACCT CCAGGGAGAA AAGGGCCCAC TGGTCTTCCA 
GGTCCCAGAG GTGAACCGGG GCCACCTGCA GATGTGGATG ACTGTCCCCG AATCCCAGGC 
CTTCCTGGGG CGCCAGGCAT GAGAGGACCA GAAGGAGCCA TGGGGCTCTC TGGAATGAGA 
GGCCCCCCAG GACCAGGGTG CAAAGGAGAG CCTGGGCTGG ATGGCAGGAG GGGTGTGGAT 
GGCGTCCCTG GGTCTCCTGG GCCTCCCGGA CGTAAAGGTG ACACAGGAGA AGACGGCTAC 
CCTGGAGGAC CAGGGCCTCC TGGTCCCATT GGGGATCCTG GGCCCAAAGG GTTTGGCCCT 
GGATACCTCG GTGGCTTCCT CCTGGTTCTC CACAGTCAGA CGGACCAGGA GCCCACCTGC 
CCCCTGGGCA TGCCCAGGCT CTGGACTGGG TATAGTCTGT TATACCTGGA AGGGCAAGAG 
AAAGCTCACA ATCAAGACCT TGGTCTGGCA GGGTCTTGCC TTCCCGTGTT TAGCACGCTG 
CCCTTTGCCT ACTGCAACAT CCACCAGGTG TGCCACTATG CCCAGAGAAA CGACAGATCC 
TACTGGCTGG CCAGCGCTGC GCCCCTCCCC ATGATGCCAC TCTCTGAAGA GGCGATCCGC 
CCCTATGTCA GCCGCTGTGC GGTATGCGAG GCCCCGGCCC AGGCGGTGGC GGTGCACAGC 
CAGGACAAGT CCATCCCCCC ATGTCCGCAG ACCTGGAGGA GCCTCTGGAT CGGGTATTCA 
TTCCTGATGC TCATGGGTGT GTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL4A4 ( XM_009444469.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_009442744.2 Pan troglodytes collagen type IV alpha 4 (COL4A4), transcript variant X9, mRNA.

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CloneID	OPc80768
Clone ID Related Accession (Same CDS sequence)	XM_016950593.1 , XM_009444469.2 , XM_009444470.2
Accession Version	XM_009444469.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4827bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	collagen alpha-4(IV) chain isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006470.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 2, 2016 this sequence version replaced XM_009444469.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGTCTC TGCACATAGT ACTAATGAGG TGCTCCTTCA GATTGACCAA GTCCTTGGCC 
ACAGATCCCT GGTCACTTAC ACTCATTCTC TTTTCTGTAC AATATGTATA TGGGAGTGGA 
AAGAAATACG TTGGTCCTTG TGGAGGAAGA GATTGCTCTG TTTGCCACTG TGTTCCTGAA 
AAGGGGTCTC GGGGTCCACC AGGACCACCA GGGCCACAGG GTCCAATTGG ACCCCTGGGA 
GCCCCAGGAC CCATTGGGCT TTCAGGAGAG AAAGGAATGA GAGGGGACCA CGGCCCTCCT 
GGAGCAGCAG GGGACAAAGG AGATAAGGGT TCAACTGGTG TTCCTGGATT TCCAGGTTTA 
GATGGCATAC CTGGGCACCC AGGGCCTCCT GGACCCAGAG GCAAACCTGG TATGAGTGGC 
CACAATGGCT CAAGAGGTGA CCCAGGGTTT CCAGGAGGAA GAGGAGCTCT TGGCCCAGGA 
GGCCCCCTAG GCCATCCTGG GGAAAAGGGA GAAAAAGGAA ATTCAGTGTT CATTTTAGGT 
GCCGTTAAAG GTATTCAGGG AGACAGAGGG GACCCAGGAC TGCCTGGCTT ACCAGGATCT 
TGGGGGGCAG GAGGACCGGC GGGTCCCACA GGATATCCTG GAGAGCCAGG GTTAGTGGGA 
CCTCCGGGCC AACCAGGGCG TCCAGGTTTG AAGGGAAATC CCGGTGTGGG AGTAAAGGGG 
CAAATGGGAG ACCCGGGTGA GGTTGGTCAG CAAGGTTCTC CTGGACCCAC CCTGTTGGTG 
GAGCCACCTG ACTTTCGTCT CTATAAAGGA GAAAAGGGTA TAAAAGGAAT TCCTGGAATG 
GTTGGACTGC CAGGACCACC AGGACGCAAG GGAGAATCTG ATATTGGGGC AAAAGGAGAA 
AAAGGTATTC CTGGATTTCC AGGGCCTCGG GGGGATCCTG GTTCCTATGG ATCTCCAGGT 
TTTCCAGGAT TAAAGGGAGA ACTAGGACTG GTTGGAGATC CTGGGCTATT TGGATTAATT 
GGCCCAAAGG GGGATCCTGG AAATCGAGGG CACCCAGGAC CACCAGGTGT TTTGGTGACT 
CCACCTCTTC CACTCAAAGG CCCACCAGGG GACCCAGGGT TCCCTGGCCG CTATGGAGAA 
ACAGGGGATG TTGGACCACC TGGTCCCCCA GGTCTCTTGG GCAGACCAGG GGAAGCCTGT 
GCAGGCATGA TAGGACCCCC TGGGCCACAA GGATTTCCTG GTCTTCCTGG GCTTCCAGGA 
GAAGCTGGTA TTCCTGGGAG ACCTGATTCT GCTCCAGGAA AACCAGGGAA GCCAGGATCA 
CCTGGCTTGC CTGGAGCACC AGGCCTGCAG GGCCTCCCAG GATCAAGTGT GATATACTGT 
AGTGTTGGGA ATCCTGGACC ACAAGGAATA AAAGGCAAAG TGGGTCCCCC AGGAGGAAGA 
GGCTCAAAAG GAGAAAAAGG AAATGAAGGA CTCTGTGCCT GTGAGCCTGG ACCCATGGGC 
CCCCCTGGCC CTCCAGGACT TCCTGGGAGG CAGGGGAGTA AGGGAGACTT GGGGCTCCCT 
GGCTGGCTTG GAACAAAAGG TGACCCAGGA CCTCCTGGTG CTGAAGGACC TCCAGGGCTA 
CCAGGAAAGC ATGGTGCCTC TGGACCACCT GGCAACAAAG GGGCGAAGGG TGACATGGTT 
GTATCAAGAG TTAAAGGGCA CAAAGGAGAA AGAGGTCCTG ATGGGCCCCC AGGATTTCCA 
GGGCAGCCAG GATCACATGG TCGGGATGGA CATGCTGGAG AAAAAGGGGA TCCAGGACCC 
CCAGGGGATC ATGAAGATGC GACCCCAGGT GGTAAAGGAT TTCCTGGACC TCTGGGCCCC 
CCGGGCAAAG CAGGACCTTT GGGGCCCCCA GGACTGGGAT TTCCTGGTCC ACCAGGACAG 
CGAGGCCACC CAGGAGTTCC AGGCCACCCA GGTGTGAGGG GCCCTGATGG CTTGAAGGGT 
CAGAAAGGTG ACACAATTTC TTGCAACGTA ACCTACCCTG GGAGGCACGG CCCTCCAGGT 
TTTGATGGAC CTCCAGGTCC GAAGGGATTT CCAGGTCCCC AAGGTGCCCC TGGGCTGAGT 
GGTTCAGATG GGCATAAAGG CAGACCTGGC ACACCAGGAA CAGCGGAAAT ACCAGGTCCA 
CCTGGTTTTC GTGGTGACAT GGGAGATCCG GGTTTTGGAG GTGAAAAGGG GTCCTCCCCT 
GCTGGGCCCC CAGGCCCTCC CGGCTCACCA GGAGTGAATG GTCAGAAAGG AATCCCGGGA 
GACCCTGCAT TTGGTCACCT GGGACCCCCG GGAAAGAGGG GTCTTTCAGG AGTGCCAGGG 
ATAAAAGGAC CCAGAGGTGA TCCGGGATGT CCAGGGGCTG AAGGGCCAGC TGGCATTCCT 
GGATTCCCAG GTCTCAAAGG TCCCAAAGGC AGAGAGGGAC ATGCTGGGTT TCCAGGTGTC 
CCAGGTCCAC CTGGCCATTC CTGTGAAAGA GGTGCTCCAG GGATACCAGG GCAACCGGGA 
CTCCCTGGGT ATCCAGGTAG CCCAGGTGCT CCAGGTGGGA AAGGACAGCT GGGAGATGTG 
GGGCCTCCCG GGCCAGCTGG AATGAAAGGC CTCCCTGGAC TCCCAGGATG GCCTGGGGCA 
CATGGTCCCC CAGGCCTCCC AGGAATCCCA GGTCCCTTTG GAGATGATGG GCTACCTGGT 
CCTCCAGGTC CAAAGGGACC CCAGGGGCTG CCTGGTTTCC CAGGTTTTCC CGGAGAAAGA 
GGAAAGCCTG GTGCAGAGGG ATGTCCTGGC ACAAAGGGAG AACCTGGAGA GAAGGGCATG 
TCTGGCCTTC CCGGAGACCG GGGACTGAGA GGGGCCAAAG GAGCCATAGG ACCTCCCGGA 
GATGAAGGAG AAATGGCTAT CATTTCCCAA AAGGGAACAC CTGGGGAACC TGGACCTCCT 
GGAGATGATG GATTCCCAGG AGAAAGAGGT GATAAAGGAA CTCCCGGGAT GCAAGGGAGA 
AGAGGAGAGC CGGGAAGATA CGGACCACCT GGATTTCAGA GAGGGGAACC TGGTGAGAAA 
GGTCAGCCAG GGCATCCTGG ACCCCCAGGC CCTCCAGGCT CAACTGGTCT AAGAGGGTTC 
ATTGGTTTTC CAGGACTTCC AGGTGACCAG GGTGAGCCAG GTTCTCCAGG TCCCCCTGGA 
TTTTCAGGAA TTGATGGAGC AAGAGGACCT AAAGGAAACA AAGGTGACCC TGCCAGTCAC 
TTTGGTCCAC CTGGTCCAAA GGGTGAGCCA GGTAGCCCTG GATGTCCAGG GCATTTTGGA 
GCATCTGGAG AGCAGGGCTT GCCTGGTATT CAAGGGCCCA GAGGATCACC TGGAAGGCCA 
GGGCCACCTG GCTCCTCTGG ACCACCAGGG TGCCCAGGTG ATCACGGGAT GCCTGGGCTG 
AGGGGACAGC CAGGAGAAAT GGGAGACCCT GGGCCAAGAG GCCTCCAGGG GGATCCAGGG 
ATACCAGGTC CTCCGGGAAT AAAAGGTCCC TCCGGATCAC CTGGCCTGAA CGGCTTGCAT 
GGATTGAAAG GTCAGAAAGG AACTAAAGGT GCTTCAGGTT TGCATGATGT GGGGCCACCT 
GGTCCAGTGG GAATACCTGG GCTAAAAGGG GAGAGAGGAG ACCCTGGGAG CCCAGGAATC 
TCTCCTCCAG GTCCTCGTGG AGAAAAAGGT CCCCCAGGAC CCCCAGGGAG TTCAGGACCA 
CCTGGTCCTG CAGGTGCCAC AGGAAGAGCT CCTAAGGACA TTCCTGACCC GGGTCCACCT 
GGAGATCAGG GACCTCCTGG TCCTGATGGC CCAAGAGGAG CACCTGGGCC TCCAGGCCTC 
CCTGGGAGTG TTGACCTTCT GAGAGGGGAG CCAGGTGACT GTGGTCTACC AGGGCCACCA 
GGTCCCCCTG GCCCACCAGG CCCTCCAGGA TACAAAGGCT TTCCAGGATG TGATGGAAAA 
GATGGCCAGA AAGGACCAAT GGGATTCCCG GGACCGCAGG GACCACATGG ATTTCCTGGG 
CCACCTGGAG AGAAGGGTTT ACCTGGACCT CCAGGGAGAA AAGGGCCCAC TGGTCTTCCA 
GGTCCCAGAG GTGAACCGGG GCCACCTGCA GATGTGGATG ACTGTCCCCG AATCCCAGGC 
CTTCCTGGGG CGCCAGGCAT GAGAGGACCA GAAGGAGCCA TGGGGCTCTC TGGAATGAGA 
GGCCCCCCAG GACCAGGGTG CAAAGGAGAG CCTGGGCTGG ATGGCAGGAG GGGTGTGGAT 
GGCGTCCCTG GGTCTCCTGG GCCTCCCGGA CGTAAAGGTG ACACAGGAGA AGACGGCTAC 
CCTGGAGGAC CAGGGCCTCC TGGTCCCATT GGGGATCCTG GGCCCAAAGG GTTTGGCCCT 
GGATACCTCG GTGGCTTCCT CCTGGTTCTC CACAGTCAGA CGGACCAGGA GCCCACCTGC 
CCCCTGGGCA TGCCCAGGCT CTGGACTGGG TATAGTCTGT TATACCTGGA AGGGCAAGAG 
AAAGCTCACA ATCAAGACCT TGGTCTGGCA GGGTCTTGCC TTCCCGTGTT TAGCACGCTG 
CCCTTTGCCT ACTGCAACAT CCACCAGGTG TGCCACTATG CCCAGAGAAA CGACAGATCC 
TACTGGCTGG CCAGCGCTGC GCCCCTCCCC ATGATGCCAC TCTCTGAAGA GGCGATCCGC 
CCCTATGTCA GCCGCTGTGC GGTATGCGAG GCCCCGGCCC AGGCGGTGGC GGTGCACAGC 
CAGGACAAGT CCATCCCCCC ATGTCCGCAG ACCTGGAGGA GCCTCTGGAT CGGGTATTCA 
TTCCTGATGC TCATGGGTGT GTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009442744.2
CDS	424..5250
Translation	MWSLHIVLMRCSFRLTKSLATDPWSLTLILFSVQYVYGSGKKYVGPCGGRDCSVCHCVPEKGSRGPPGPPGPQGPIGPLGAPGPIGLSGEKGMRGDHGPPGAAGDKGDKGSTGVPGFPGLDGIPGHPGPPGPRGKPGMSGHNGSRGDPGFPGGRGALGPGGPLGHPGEKGEKGNSVFILGAVKGIQGDRGDPGLPGLPGSWGAGGPAGPTGYPGEPGLVGPPGQPGRPGLKGNPGVGVKGQMGDPGEVGQQGSPGPTLLVEPPDFRLYKGEKGIKGIPGMVGLPGPPGRKGESDIGAKGEKGIPGFPGPRGDPGSYGSPGFPGLKGELGLVGDPGLFGLIGPKGDPGNRGHPGPPGVLVTPPLPLKGPPGDPGFPGRYGETGDVGPPGPPGLLGRPGEACAGMIGPPGPQGFPGLPGLPGEAGIPGRPDSAPGKPGKPGSPGLPGAPGLQGLPGSSVIYCSVGNPGPQGIKGKVGPPGGRGSKGEKGNEGLCACEPGPMGPPGPPGLPGRQGSKGDLGLPGWLGTKGDPGPPGAEGPPGLPGKHGASGPPGNKGAKGDMVVSRVKGHKGERGPDGPPGFPGQPGSHGRDGHAGEKGDPGPPGDHEDATPGGKGFPGPLGPPGKAGPLGPPGLGFPGPPGQRGHPGVPGHPGVRGPDGLKGQKGDTISCNVTYPGRHGPPGFDGPPGPKGFPGPQGAPGLSGSDGHKGRPGTPGTAEIPGPPGFRGDMGDPGFGGEKGSSPAGPPGPPGSPGVNGQKGIPGDPAFGHLGPPGKRGLSGVPGIKGPRGDPGCPGAEGPAGIPGFPGLKGPKGREGHAGFPGVPGPPGHSCERGAPGIPGQPGLPGYPGSPGAPGGKGQLGDVGPPGPAGMKGLPGLPGWPGAHGPPGLPGIPGPFGDDGLPGPPGPKGPQGLPGFPGFPGERGKPGAEGCPGTKGEPGEKGMSGLPGDRGLRGAKGAIGPPGDEGEMAIISQKGTPGEPGPPGDDGFPGERGDKGTPGMQGRRGEPGRYGPPGFQRGEPGEKGQPGHPGPPGPPGSTGLRGFIGFPGLPGDQGEPGSPGPPGFSGIDGARGPKGNKGDPASHFGPPGPKGEPGSPGCPGHFGASGEQGLPGIQGPRGSPGRPGPPGSSGPPGCPGDHGMPGLRGQPGEMGDPGPRGLQGDPGIPGPPGIKGPSGSPGLNGLHGLKGQKGTKGASGLHDVGPPGPVGIPGLKGERGDPGSPGISPPGPRGEKGPPGPPGSSGPPGPAGATGRAPKDIPDPGPPGDQGPPGPDGPRGAPGPPGLPGSVDLLRGEPGDCGLPGPPGPPGPPGPPGYKGFPGCDGKDGQKGPMGFPGPQGPHGFPGPPGEKGLPGPPGRKGPTGLPGPRGEPGPPADVDDCPRIPGLPGAPGMRGPEGAMGLSGMRGPPGPGCKGEPGLDGRRGVDGVPGSPGPPGRKGDTGEDGYPGGPGPPGPIGDPGPKGFGPGYLGGFLLVLHSQTDQEPTCPLGMPRLWTGYSLLYLEGQEKAHNQDLGLAGSCLPVFSTLPFAYCNIHQVCHYAQRNDRSYWLASAAPLPMMPLSEEAIRPYVSRCAVCEAPAQAVAVHSQDKSIPPCPQTWRSLWIGYSFLMLMGVF

Target ORF information:

RefSeq Version	XM_009444469.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes collagen type IV alpha 4 (COL4A4), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009444469.2

ORF Insert Sequence:

ATGTGGTCTC TGCACATAGT ACTAATGAGG TGCTCCTTCA GATTGACCAA GTCCTTGGCC 
ACAGATCCCT GGTCACTTAC ACTCATTCTC TTTTCTGTAC AATATGTATA TGGGAGTGGA 
AAGAAATACG TTGGTCCTTG TGGAGGAAGA GATTGCTCTG TTTGCCACTG TGTTCCTGAA 
AAGGGGTCTC GGGGTCCACC AGGACCACCA GGGCCACAGG GTCCAATTGG ACCCCTGGGA 
GCCCCAGGAC CCATTGGGCT TTCAGGAGAG AAAGGAATGA GAGGGGACCA CGGCCCTCCT 
GGAGCAGCAG GGGACAAAGG AGATAAGGGT TCAACTGGTG TTCCTGGATT TCCAGGTTTA 
GATGGCATAC CTGGGCACCC AGGGCCTCCT GGACCCAGAG GCAAACCTGG TATGAGTGGC 
CACAATGGCT CAAGAGGTGA CCCAGGGTTT CCAGGAGGAA GAGGAGCTCT TGGCCCAGGA 
GGCCCCCTAG GCCATCCTGG GGAAAAGGGA GAAAAAGGAA ATTCAGTGTT CATTTTAGGT 
GCCGTTAAAG GTATTCAGGG AGACAGAGGG GACCCAGGAC TGCCTGGCTT ACCAGGATCT 
TGGGGGGCAG GAGGACCGGC GGGTCCCACA GGATATCCTG GAGAGCCAGG GTTAGTGGGA 
CCTCCGGGCC AACCAGGGCG TCCAGGTTTG AAGGGAAATC CCGGTGTGGG AGTAAAGGGG 
CAAATGGGAG ACCCGGGTGA GGTTGGTCAG CAAGGTTCTC CTGGACCCAC CCTGTTGGTG 
GAGCCACCTG ACTTTCGTCT CTATAAAGGA GAAAAGGGTA TAAAAGGAAT TCCTGGAATG 
GTTGGACTGC CAGGACCACC AGGACGCAAG GGAGAATCTG ATATTGGGGC AAAAGGAGAA 
AAAGGTATTC CTGGATTTCC AGGGCCTCGG GGGGATCCTG GTTCCTATGG ATCTCCAGGT 
TTTCCAGGAT TAAAGGGAGA ACTAGGACTG GTTGGAGATC CTGGGCTATT TGGATTAATT 
GGCCCAAAGG GGGATCCTGG AAATCGAGGG CACCCAGGAC CACCAGGTGT TTTGGTGACT 
CCACCTCTTC CACTCAAAGG CCCACCAGGG GACCCAGGGT TCCCTGGCCG CTATGGAGAA 
ACAGGGGATG TTGGACCACC TGGTCCCCCA GGTCTCTTGG GCAGACCAGG GGAAGCCTGT 
GCAGGCATGA TAGGACCCCC TGGGCCACAA GGATTTCCTG GTCTTCCTGG GCTTCCAGGA 
GAAGCTGGTA TTCCTGGGAG ACCTGATTCT GCTCCAGGAA AACCAGGGAA GCCAGGATCA 
CCTGGCTTGC CTGGAGCACC AGGCCTGCAG GGCCTCCCAG GATCAAGTGT GATATACTGT 
AGTGTTGGGA ATCCTGGACC ACAAGGAATA AAAGGCAAAG TGGGTCCCCC AGGAGGAAGA 
GGCTCAAAAG GAGAAAAAGG AAATGAAGGA CTCTGTGCCT GTGAGCCTGG ACCCATGGGC 
CCCCCTGGCC CTCCAGGACT TCCTGGGAGG CAGGGGAGTA AGGGAGACTT GGGGCTCCCT 
GGCTGGCTTG GAACAAAAGG TGACCCAGGA CCTCCTGGTG CTGAAGGACC TCCAGGGCTA 
CCAGGAAAGC ATGGTGCCTC TGGACCACCT GGCAACAAAG GGGCGAAGGG TGACATGGTT 
GTATCAAGAG TTAAAGGGCA CAAAGGAGAA AGAGGTCCTG ATGGGCCCCC AGGATTTCCA 
GGGCAGCCAG GATCACATGG TCGGGATGGA CATGCTGGAG AAAAAGGGGA TCCAGGACCC 
CCAGGGGATC ATGAAGATGC GACCCCAGGT GGTAAAGGAT TTCCTGGACC TCTGGGCCCC 
CCGGGCAAAG CAGGACCTTT GGGGCCCCCA GGACTGGGAT TTCCTGGTCC ACCAGGACAG 
CGAGGCCACC CAGGAGTTCC AGGCCACCCA GGTGTGAGGG GCCCTGATGG CTTGAAGGGT 
CAGAAAGGTG ACACAATTTC TTGCAACGTA ACCTACCCTG GGAGGCACGG CCCTCCAGGT 
TTTGATGGAC CTCCAGGTCC GAAGGGATTT CCAGGTCCCC AAGGTGCCCC TGGGCTGAGT 
GGTTCAGATG GGCATAAAGG CAGACCTGGC ACACCAGGAA CAGCGGAAAT ACCAGGTCCA 
CCTGGTTTTC GTGGTGACAT GGGAGATCCG GGTTTTGGAG GTGAAAAGGG GTCCTCCCCT 
GCTGGGCCCC CAGGCCCTCC CGGCTCACCA GGAGTGAATG GTCAGAAAGG AATCCCGGGA 
GACCCTGCAT TTGGTCACCT GGGACCCCCG GGAAAGAGGG GTCTTTCAGG AGTGCCAGGG 
ATAAAAGGAC CCAGAGGTGA TCCGGGATGT CCAGGGGCTG AAGGGCCAGC TGGCATTCCT 
GGATTCCCAG GTCTCAAAGG TCCCAAAGGC AGAGAGGGAC ATGCTGGGTT TCCAGGTGTC 
CCAGGTCCAC CTGGCCATTC CTGTGAAAGA GGTGCTCCAG GGATACCAGG GCAACCGGGA 
CTCCCTGGGT ATCCAGGTAG CCCAGGTGCT CCAGGTGGGA AAGGACAGCT GGGAGATGTG 
GGGCCTCCCG GGCCAGCTGG AATGAAAGGC CTCCCTGGAC TCCCAGGATG GCCTGGGGCA 
CATGGTCCCC CAGGCCTCCC AGGAATCCCA GGTCCCTTTG GAGATGATGG GCTACCTGGT 
CCTCCAGGTC CAAAGGGACC CCAGGGGCTG CCTGGTTTCC CAGGTTTTCC CGGAGAAAGA 
GGAAAGCCTG GTGCAGAGGG ATGTCCTGGC ACAAAGGGAG AACCTGGAGA GAAGGGCATG 
TCTGGCCTTC CCGGAGACCG GGGACTGAGA GGGGCCAAAG GAGCCATAGG ACCTCCCGGA 
GATGAAGGAG AAATGGCTAT CATTTCCCAA AAGGGAACAC CTGGGGAACC TGGACCTCCT 
GGAGATGATG GATTCCCAGG AGAAAGAGGT GATAAAGGAA CTCCCGGGAT GCAAGGGAGA 
AGAGGAGAGC CGGGAAGATA CGGACCACCT GGATTTCAGA GAGGGGAACC TGGTGAGAAA 
GGTCAGCCAG GGCATCCTGG ACCCCCAGGC CCTCCAGGCT CAACTGGTCT AAGAGGGTTC 
ATTGGTTTTC CAGGACTTCC AGGTGACCAG GGTGAGCCAG GTTCTCCAGG TCCCCCTGGA 
TTTTCAGGAA TTGATGGAGC AAGAGGACCT AAAGGAAACA AAGGTGACCC TGCCAGTCAC 
TTTGGTCCAC CTGGTCCAAA GGGTGAGCCA GGTAGCCCTG GATGTCCAGG GCATTTTGGA 
GCATCTGGAG AGCAGGGCTT GCCTGGTATT CAAGGGCCCA GAGGATCACC TGGAAGGCCA 
GGGCCACCTG GCTCCTCTGG ACCACCAGGG TGCCCAGGTG ATCACGGGAT GCCTGGGCTG 
AGGGGACAGC CAGGAGAAAT GGGAGACCCT GGGCCAAGAG GCCTCCAGGG GGATCCAGGG 
ATACCAGGTC CTCCGGGAAT AAAAGGTCCC TCCGGATCAC CTGGCCTGAA CGGCTTGCAT 
GGATTGAAAG GTCAGAAAGG AACTAAAGGT GCTTCAGGTT TGCATGATGT GGGGCCACCT 
GGTCCAGTGG GAATACCTGG GCTAAAAGGG GAGAGAGGAG ACCCTGGGAG CCCAGGAATC 
TCTCCTCCAG GTCCTCGTGG AGAAAAAGGT CCCCCAGGAC CCCCAGGGAG TTCAGGACCA 
CCTGGTCCTG CAGGTGCCAC AGGAAGAGCT CCTAAGGACA TTCCTGACCC GGGTCCACCT 
GGAGATCAGG GACCTCCTGG TCCTGATGGC CCAAGAGGAG CACCTGGGCC TCCAGGCCTC 
CCTGGGAGTG TTGACCTTCT GAGAGGGGAG CCAGGTGACT GTGGTCTACC AGGGCCACCA 
GGTCCCCCTG GCCCACCAGG CCCTCCAGGA TACAAAGGCT TTCCAGGATG TGATGGAAAA 
GATGGCCAGA AAGGACCAAT GGGATTCCCG GGACCGCAGG GACCACATGG ATTTCCTGGG 
CCACCTGGAG AGAAGGGTTT ACCTGGACCT CCAGGGAGAA AAGGGCCCAC TGGTCTTCCA 
GGTCCCAGAG GTGAACCGGG GCCACCTGCA GATGTGGATG ACTGTCCCCG AATCCCAGGC 
CTTCCTGGGG CGCCAGGCAT GAGAGGACCA GAAGGAGCCA TGGGGCTCTC TGGAATGAGA 
GGCCCCCCAG GACCAGGGTG CAAAGGAGAG CCTGGGCTGG ATGGCAGGAG GGGTGTGGAT 
GGCGTCCCTG GGTCTCCTGG GCCTCCCGGA CGTAAAGGTG ACACAGGAGA AGACGGCTAC 
CCTGGAGGAC CAGGGCCTCC TGGTCCCATT GGGGATCCTG GGCCCAAAGG GTTTGGCCCT 
GGATACCTCG GTGGCTTCCT CCTGGTTCTC CACAGTCAGA CGGACCAGGA GCCCACCTGC 
CCCCTGGGCA TGCCCAGGCT CTGGACTGGG TATAGTCTGT TATACCTGGA AGGGCAAGAG 
AAAGCTCACA ATCAAGACCT TGGTCTGGCA GGGTCTTGCC TTCCCGTGTT TAGCACGCTG 
CCCTTTGCCT ACTGCAACAT CCACCAGGTG TGCCACTATG CCCAGAGAAA CGACAGATCC 
TACTGGCTGG CCAGCGCTGC GCCCCTCCCC ATGATGCCAC TCTCTGAAGA GGCGATCCGC 
CCCTATGTCA GCCGCTGTGC GGTATGCGAG GCCCCGGCCC AGGCGGTGGC GGTGCACAGC 
CAGGACAAGT CCATCCCCCC ATGTCCGCAG ACCTGGAGGA GCCTCTGGAT CGGGTATTCA 
TTCCTGATGC TCATGGGTGT GTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL4A4 ( XM_009444470.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_009442745.2 Pan troglodytes collagen type IV alpha 4 (COL4A4), transcript variant X11, mRNA.

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CloneID	OPc80768
Clone ID Related Accession (Same CDS sequence)	XM_016950593.1 , XM_009444469.2 , XM_009444470.2
Accession Version	XM_009444470.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4827bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	collagen alpha-4(IV) chain isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006470.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 2, 2016 this sequence version replaced XM_009444470.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGTCTC TGCACATAGT ACTAATGAGG TGCTCCTTCA GATTGACCAA GTCCTTGGCC 
ACAGATCCCT GGTCACTTAC ACTCATTCTC TTTTCTGTAC AATATGTATA TGGGAGTGGA 
AAGAAATACG TTGGTCCTTG TGGAGGAAGA GATTGCTCTG TTTGCCACTG TGTTCCTGAA 
AAGGGGTCTC GGGGTCCACC AGGACCACCA GGGCCACAGG GTCCAATTGG ACCCCTGGGA 
GCCCCAGGAC CCATTGGGCT TTCAGGAGAG AAAGGAATGA GAGGGGACCA CGGCCCTCCT 
GGAGCAGCAG GGGACAAAGG AGATAAGGGT TCAACTGGTG TTCCTGGATT TCCAGGTTTA 
GATGGCATAC CTGGGCACCC AGGGCCTCCT GGACCCAGAG GCAAACCTGG TATGAGTGGC 
CACAATGGCT CAAGAGGTGA CCCAGGGTTT CCAGGAGGAA GAGGAGCTCT TGGCCCAGGA 
GGCCCCCTAG GCCATCCTGG GGAAAAGGGA GAAAAAGGAA ATTCAGTGTT CATTTTAGGT 
GCCGTTAAAG GTATTCAGGG AGACAGAGGG GACCCAGGAC TGCCTGGCTT ACCAGGATCT 
TGGGGGGCAG GAGGACCGGC GGGTCCCACA GGATATCCTG GAGAGCCAGG GTTAGTGGGA 
CCTCCGGGCC AACCAGGGCG TCCAGGTTTG AAGGGAAATC CCGGTGTGGG AGTAAAGGGG 
CAAATGGGAG ACCCGGGTGA GGTTGGTCAG CAAGGTTCTC CTGGACCCAC CCTGTTGGTG 
GAGCCACCTG ACTTTCGTCT CTATAAAGGA GAAAAGGGTA TAAAAGGAAT TCCTGGAATG 
GTTGGACTGC CAGGACCACC AGGACGCAAG GGAGAATCTG ATATTGGGGC AAAAGGAGAA 
AAAGGTATTC CTGGATTTCC AGGGCCTCGG GGGGATCCTG GTTCCTATGG ATCTCCAGGT 
TTTCCAGGAT TAAAGGGAGA ACTAGGACTG GTTGGAGATC CTGGGCTATT TGGATTAATT 
GGCCCAAAGG GGGATCCTGG AAATCGAGGG CACCCAGGAC CACCAGGTGT TTTGGTGACT 
CCACCTCTTC CACTCAAAGG CCCACCAGGG GACCCAGGGT TCCCTGGCCG CTATGGAGAA 
ACAGGGGATG TTGGACCACC TGGTCCCCCA GGTCTCTTGG GCAGACCAGG GGAAGCCTGT 
GCAGGCATGA TAGGACCCCC TGGGCCACAA GGATTTCCTG GTCTTCCTGG GCTTCCAGGA 
GAAGCTGGTA TTCCTGGGAG ACCTGATTCT GCTCCAGGAA AACCAGGGAA GCCAGGATCA 
CCTGGCTTGC CTGGAGCACC AGGCCTGCAG GGCCTCCCAG GATCAAGTGT GATATACTGT 
AGTGTTGGGA ATCCTGGACC ACAAGGAATA AAAGGCAAAG TGGGTCCCCC AGGAGGAAGA 
GGCTCAAAAG GAGAAAAAGG AAATGAAGGA CTCTGTGCCT GTGAGCCTGG ACCCATGGGC 
CCCCCTGGCC CTCCAGGACT TCCTGGGAGG CAGGGGAGTA AGGGAGACTT GGGGCTCCCT 
GGCTGGCTTG GAACAAAAGG TGACCCAGGA CCTCCTGGTG CTGAAGGACC TCCAGGGCTA 
CCAGGAAAGC ATGGTGCCTC TGGACCACCT GGCAACAAAG GGGCGAAGGG TGACATGGTT 
GTATCAAGAG TTAAAGGGCA CAAAGGAGAA AGAGGTCCTG ATGGGCCCCC AGGATTTCCA 
GGGCAGCCAG GATCACATGG TCGGGATGGA CATGCTGGAG AAAAAGGGGA TCCAGGACCC 
CCAGGGGATC ATGAAGATGC GACCCCAGGT GGTAAAGGAT TTCCTGGACC TCTGGGCCCC 
CCGGGCAAAG CAGGACCTTT GGGGCCCCCA GGACTGGGAT TTCCTGGTCC ACCAGGACAG 
CGAGGCCACC CAGGAGTTCC AGGCCACCCA GGTGTGAGGG GCCCTGATGG CTTGAAGGGT 
CAGAAAGGTG ACACAATTTC TTGCAACGTA ACCTACCCTG GGAGGCACGG CCCTCCAGGT 
TTTGATGGAC CTCCAGGTCC GAAGGGATTT CCAGGTCCCC AAGGTGCCCC TGGGCTGAGT 
GGTTCAGATG GGCATAAAGG CAGACCTGGC ACACCAGGAA CAGCGGAAAT ACCAGGTCCA 
CCTGGTTTTC GTGGTGACAT GGGAGATCCG GGTTTTGGAG GTGAAAAGGG GTCCTCCCCT 
GCTGGGCCCC CAGGCCCTCC CGGCTCACCA GGAGTGAATG GTCAGAAAGG AATCCCGGGA 
GACCCTGCAT TTGGTCACCT GGGACCCCCG GGAAAGAGGG GTCTTTCAGG AGTGCCAGGG 
ATAAAAGGAC CCAGAGGTGA TCCGGGATGT CCAGGGGCTG AAGGGCCAGC TGGCATTCCT 
GGATTCCCAG GTCTCAAAGG TCCCAAAGGC AGAGAGGGAC ATGCTGGGTT TCCAGGTGTC 
CCAGGTCCAC CTGGCCATTC CTGTGAAAGA GGTGCTCCAG GGATACCAGG GCAACCGGGA 
CTCCCTGGGT ATCCAGGTAG CCCAGGTGCT CCAGGTGGGA AAGGACAGCT GGGAGATGTG 
GGGCCTCCCG GGCCAGCTGG AATGAAAGGC CTCCCTGGAC TCCCAGGATG GCCTGGGGCA 
CATGGTCCCC CAGGCCTCCC AGGAATCCCA GGTCCCTTTG GAGATGATGG GCTACCTGGT 
CCTCCAGGTC CAAAGGGACC CCAGGGGCTG CCTGGTTTCC CAGGTTTTCC CGGAGAAAGA 
GGAAAGCCTG GTGCAGAGGG ATGTCCTGGC ACAAAGGGAG AACCTGGAGA GAAGGGCATG 
TCTGGCCTTC CCGGAGACCG GGGACTGAGA GGGGCCAAAG GAGCCATAGG ACCTCCCGGA 
GATGAAGGAG AAATGGCTAT CATTTCCCAA AAGGGAACAC CTGGGGAACC TGGACCTCCT 
GGAGATGATG GATTCCCAGG AGAAAGAGGT GATAAAGGAA CTCCCGGGAT GCAAGGGAGA 
AGAGGAGAGC CGGGAAGATA CGGACCACCT GGATTTCAGA GAGGGGAACC TGGTGAGAAA 
GGTCAGCCAG GGCATCCTGG ACCCCCAGGC CCTCCAGGCT CAACTGGTCT AAGAGGGTTC 
ATTGGTTTTC CAGGACTTCC AGGTGACCAG GGTGAGCCAG GTTCTCCAGG TCCCCCTGGA 
TTTTCAGGAA TTGATGGAGC AAGAGGACCT AAAGGAAACA AAGGTGACCC TGCCAGTCAC 
TTTGGTCCAC CTGGTCCAAA GGGTGAGCCA GGTAGCCCTG GATGTCCAGG GCATTTTGGA 
GCATCTGGAG AGCAGGGCTT GCCTGGTATT CAAGGGCCCA GAGGATCACC TGGAAGGCCA 
GGGCCACCTG GCTCCTCTGG ACCACCAGGG TGCCCAGGTG ATCACGGGAT GCCTGGGCTG 
AGGGGACAGC CAGGAGAAAT GGGAGACCCT GGGCCAAGAG GCCTCCAGGG GGATCCAGGG 
ATACCAGGTC CTCCGGGAAT AAAAGGTCCC TCCGGATCAC CTGGCCTGAA CGGCTTGCAT 
GGATTGAAAG GTCAGAAAGG AACTAAAGGT GCTTCAGGTT TGCATGATGT GGGGCCACCT 
GGTCCAGTGG GAATACCTGG GCTAAAAGGG GAGAGAGGAG ACCCTGGGAG CCCAGGAATC 
TCTCCTCCAG GTCCTCGTGG AGAAAAAGGT CCCCCAGGAC CCCCAGGGAG TTCAGGACCA 
CCTGGTCCTG CAGGTGCCAC AGGAAGAGCT CCTAAGGACA TTCCTGACCC GGGTCCACCT 
GGAGATCAGG GACCTCCTGG TCCTGATGGC CCAAGAGGAG CACCTGGGCC TCCAGGCCTC 
CCTGGGAGTG TTGACCTTCT GAGAGGGGAG CCAGGTGACT GTGGTCTACC AGGGCCACCA 
GGTCCCCCTG GCCCACCAGG CCCTCCAGGA TACAAAGGCT TTCCAGGATG TGATGGAAAA 
GATGGCCAGA AAGGACCAAT GGGATTCCCG GGACCGCAGG GACCACATGG ATTTCCTGGG 
CCACCTGGAG AGAAGGGTTT ACCTGGACCT CCAGGGAGAA AAGGGCCCAC TGGTCTTCCA 
GGTCCCAGAG GTGAACCGGG GCCACCTGCA GATGTGGATG ACTGTCCCCG AATCCCAGGC 
CTTCCTGGGG CGCCAGGCAT GAGAGGACCA GAAGGAGCCA TGGGGCTCTC TGGAATGAGA 
GGCCCCCCAG GACCAGGGTG CAAAGGAGAG CCTGGGCTGG ATGGCAGGAG GGGTGTGGAT 
GGCGTCCCTG GGTCTCCTGG GCCTCCCGGA CGTAAAGGTG ACACAGGAGA AGACGGCTAC 
CCTGGAGGAC CAGGGCCTCC TGGTCCCATT GGGGATCCTG GGCCCAAAGG GTTTGGCCCT 
GGATACCTCG GTGGCTTCCT CCTGGTTCTC CACAGTCAGA CGGACCAGGA GCCCACCTGC 
CCCCTGGGCA TGCCCAGGCT CTGGACTGGG TATAGTCTGT TATACCTGGA AGGGCAAGAG 
AAAGCTCACA ATCAAGACCT TGGTCTGGCA GGGTCTTGCC TTCCCGTGTT TAGCACGCTG 
CCCTTTGCCT ACTGCAACAT CCACCAGGTG TGCCACTATG CCCAGAGAAA CGACAGATCC 
TACTGGCTGG CCAGCGCTGC GCCCCTCCCC ATGATGCCAC TCTCTGAAGA GGCGATCCGC 
CCCTATGTCA GCCGCTGTGC GGTATGCGAG GCCCCGGCCC AGGCGGTGGC GGTGCACAGC 
CAGGACAAGT CCATCCCCCC ATGTCCGCAG ACCTGGAGGA GCCTCTGGAT CGGGTATTCA 
TTCCTGATGC TCATGGGTGT GTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009442745.2
CDS	424..5250
Translation	MWSLHIVLMRCSFRLTKSLATDPWSLTLILFSVQYVYGSGKKYVGPCGGRDCSVCHCVPEKGSRGPPGPPGPQGPIGPLGAPGPIGLSGEKGMRGDHGPPGAAGDKGDKGSTGVPGFPGLDGIPGHPGPPGPRGKPGMSGHNGSRGDPGFPGGRGALGPGGPLGHPGEKGEKGNSVFILGAVKGIQGDRGDPGLPGLPGSWGAGGPAGPTGYPGEPGLVGPPGQPGRPGLKGNPGVGVKGQMGDPGEVGQQGSPGPTLLVEPPDFRLYKGEKGIKGIPGMVGLPGPPGRKGESDIGAKGEKGIPGFPGPRGDPGSYGSPGFPGLKGELGLVGDPGLFGLIGPKGDPGNRGHPGPPGVLVTPPLPLKGPPGDPGFPGRYGETGDVGPPGPPGLLGRPGEACAGMIGPPGPQGFPGLPGLPGEAGIPGRPDSAPGKPGKPGSPGLPGAPGLQGLPGSSVIYCSVGNPGPQGIKGKVGPPGGRGSKGEKGNEGLCACEPGPMGPPGPPGLPGRQGSKGDLGLPGWLGTKGDPGPPGAEGPPGLPGKHGASGPPGNKGAKGDMVVSRVKGHKGERGPDGPPGFPGQPGSHGRDGHAGEKGDPGPPGDHEDATPGGKGFPGPLGPPGKAGPLGPPGLGFPGPPGQRGHPGVPGHPGVRGPDGLKGQKGDTISCNVTYPGRHGPPGFDGPPGPKGFPGPQGAPGLSGSDGHKGRPGTPGTAEIPGPPGFRGDMGDPGFGGEKGSSPAGPPGPPGSPGVNGQKGIPGDPAFGHLGPPGKRGLSGVPGIKGPRGDPGCPGAEGPAGIPGFPGLKGPKGREGHAGFPGVPGPPGHSCERGAPGIPGQPGLPGYPGSPGAPGGKGQLGDVGPPGPAGMKGLPGLPGWPGAHGPPGLPGIPGPFGDDGLPGPPGPKGPQGLPGFPGFPGERGKPGAEGCPGTKGEPGEKGMSGLPGDRGLRGAKGAIGPPGDEGEMAIISQKGTPGEPGPPGDDGFPGERGDKGTPGMQGRRGEPGRYGPPGFQRGEPGEKGQPGHPGPPGPPGSTGLRGFIGFPGLPGDQGEPGSPGPPGFSGIDGARGPKGNKGDPASHFGPPGPKGEPGSPGCPGHFGASGEQGLPGIQGPRGSPGRPGPPGSSGPPGCPGDHGMPGLRGQPGEMGDPGPRGLQGDPGIPGPPGIKGPSGSPGLNGLHGLKGQKGTKGASGLHDVGPPGPVGIPGLKGERGDPGSPGISPPGPRGEKGPPGPPGSSGPPGPAGATGRAPKDIPDPGPPGDQGPPGPDGPRGAPGPPGLPGSVDLLRGEPGDCGLPGPPGPPGPPGPPGYKGFPGCDGKDGQKGPMGFPGPQGPHGFPGPPGEKGLPGPPGRKGPTGLPGPRGEPGPPADVDDCPRIPGLPGAPGMRGPEGAMGLSGMRGPPGPGCKGEPGLDGRRGVDGVPGSPGPPGRKGDTGEDGYPGGPGPPGPIGDPGPKGFGPGYLGGFLLVLHSQTDQEPTCPLGMPRLWTGYSLLYLEGQEKAHNQDLGLAGSCLPVFSTLPFAYCNIHQVCHYAQRNDRSYWLASAAPLPMMPLSEEAIRPYVSRCAVCEAPAQAVAVHSQDKSIPPCPQTWRSLWIGYSFLMLMGVF

Target ORF information:

RefSeq Version	XM_009444470.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes collagen type IV alpha 4 (COL4A4), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009444470.2

ORF Insert Sequence:

ATGTGGTCTC TGCACATAGT ACTAATGAGG TGCTCCTTCA GATTGACCAA GTCCTTGGCC 
ACAGATCCCT GGTCACTTAC ACTCATTCTC TTTTCTGTAC AATATGTATA TGGGAGTGGA 
AAGAAATACG TTGGTCCTTG TGGAGGAAGA GATTGCTCTG TTTGCCACTG TGTTCCTGAA 
AAGGGGTCTC GGGGTCCACC AGGACCACCA GGGCCACAGG GTCCAATTGG ACCCCTGGGA 
GCCCCAGGAC CCATTGGGCT TTCAGGAGAG AAAGGAATGA GAGGGGACCA CGGCCCTCCT 
GGAGCAGCAG GGGACAAAGG AGATAAGGGT TCAACTGGTG TTCCTGGATT TCCAGGTTTA 
GATGGCATAC CTGGGCACCC AGGGCCTCCT GGACCCAGAG GCAAACCTGG TATGAGTGGC 
CACAATGGCT CAAGAGGTGA CCCAGGGTTT CCAGGAGGAA GAGGAGCTCT TGGCCCAGGA 
GGCCCCCTAG GCCATCCTGG GGAAAAGGGA GAAAAAGGAA ATTCAGTGTT CATTTTAGGT 
GCCGTTAAAG GTATTCAGGG AGACAGAGGG GACCCAGGAC TGCCTGGCTT ACCAGGATCT 
TGGGGGGCAG GAGGACCGGC GGGTCCCACA GGATATCCTG GAGAGCCAGG GTTAGTGGGA 
CCTCCGGGCC AACCAGGGCG TCCAGGTTTG AAGGGAAATC CCGGTGTGGG AGTAAAGGGG 
CAAATGGGAG ACCCGGGTGA GGTTGGTCAG CAAGGTTCTC CTGGACCCAC CCTGTTGGTG 
GAGCCACCTG ACTTTCGTCT CTATAAAGGA GAAAAGGGTA TAAAAGGAAT TCCTGGAATG 
GTTGGACTGC CAGGACCACC AGGACGCAAG GGAGAATCTG ATATTGGGGC AAAAGGAGAA 
AAAGGTATTC CTGGATTTCC AGGGCCTCGG GGGGATCCTG GTTCCTATGG ATCTCCAGGT 
TTTCCAGGAT TAAAGGGAGA ACTAGGACTG GTTGGAGATC CTGGGCTATT TGGATTAATT 
GGCCCAAAGG GGGATCCTGG AAATCGAGGG CACCCAGGAC CACCAGGTGT TTTGGTGACT 
CCACCTCTTC CACTCAAAGG CCCACCAGGG GACCCAGGGT TCCCTGGCCG CTATGGAGAA 
ACAGGGGATG TTGGACCACC TGGTCCCCCA GGTCTCTTGG GCAGACCAGG GGAAGCCTGT 
GCAGGCATGA TAGGACCCCC TGGGCCACAA GGATTTCCTG GTCTTCCTGG GCTTCCAGGA 
GAAGCTGGTA TTCCTGGGAG ACCTGATTCT GCTCCAGGAA AACCAGGGAA GCCAGGATCA 
CCTGGCTTGC CTGGAGCACC AGGCCTGCAG GGCCTCCCAG GATCAAGTGT GATATACTGT 
AGTGTTGGGA ATCCTGGACC ACAAGGAATA AAAGGCAAAG TGGGTCCCCC AGGAGGAAGA 
GGCTCAAAAG GAGAAAAAGG AAATGAAGGA CTCTGTGCCT GTGAGCCTGG ACCCATGGGC 
CCCCCTGGCC CTCCAGGACT TCCTGGGAGG CAGGGGAGTA AGGGAGACTT GGGGCTCCCT 
GGCTGGCTTG GAACAAAAGG TGACCCAGGA CCTCCTGGTG CTGAAGGACC TCCAGGGCTA 
CCAGGAAAGC ATGGTGCCTC TGGACCACCT GGCAACAAAG GGGCGAAGGG TGACATGGTT 
GTATCAAGAG TTAAAGGGCA CAAAGGAGAA AGAGGTCCTG ATGGGCCCCC AGGATTTCCA 
GGGCAGCCAG GATCACATGG TCGGGATGGA CATGCTGGAG AAAAAGGGGA TCCAGGACCC 
CCAGGGGATC ATGAAGATGC GACCCCAGGT GGTAAAGGAT TTCCTGGACC TCTGGGCCCC 
CCGGGCAAAG CAGGACCTTT GGGGCCCCCA GGACTGGGAT TTCCTGGTCC ACCAGGACAG 
CGAGGCCACC CAGGAGTTCC AGGCCACCCA GGTGTGAGGG GCCCTGATGG CTTGAAGGGT 
CAGAAAGGTG ACACAATTTC TTGCAACGTA ACCTACCCTG GGAGGCACGG CCCTCCAGGT 
TTTGATGGAC CTCCAGGTCC GAAGGGATTT CCAGGTCCCC AAGGTGCCCC TGGGCTGAGT 
GGTTCAGATG GGCATAAAGG CAGACCTGGC ACACCAGGAA CAGCGGAAAT ACCAGGTCCA 
CCTGGTTTTC GTGGTGACAT GGGAGATCCG GGTTTTGGAG GTGAAAAGGG GTCCTCCCCT 
GCTGGGCCCC CAGGCCCTCC CGGCTCACCA GGAGTGAATG GTCAGAAAGG AATCCCGGGA 
GACCCTGCAT TTGGTCACCT GGGACCCCCG GGAAAGAGGG GTCTTTCAGG AGTGCCAGGG 
ATAAAAGGAC CCAGAGGTGA TCCGGGATGT CCAGGGGCTG AAGGGCCAGC TGGCATTCCT 
GGATTCCCAG GTCTCAAAGG TCCCAAAGGC AGAGAGGGAC ATGCTGGGTT TCCAGGTGTC 
CCAGGTCCAC CTGGCCATTC CTGTGAAAGA GGTGCTCCAG GGATACCAGG GCAACCGGGA 
CTCCCTGGGT ATCCAGGTAG CCCAGGTGCT CCAGGTGGGA AAGGACAGCT GGGAGATGTG 
GGGCCTCCCG GGCCAGCTGG AATGAAAGGC CTCCCTGGAC TCCCAGGATG GCCTGGGGCA 
CATGGTCCCC CAGGCCTCCC AGGAATCCCA GGTCCCTTTG GAGATGATGG GCTACCTGGT 
CCTCCAGGTC CAAAGGGACC CCAGGGGCTG CCTGGTTTCC CAGGTTTTCC CGGAGAAAGA 
GGAAAGCCTG GTGCAGAGGG ATGTCCTGGC ACAAAGGGAG AACCTGGAGA GAAGGGCATG 
TCTGGCCTTC CCGGAGACCG GGGACTGAGA GGGGCCAAAG GAGCCATAGG ACCTCCCGGA 
GATGAAGGAG AAATGGCTAT CATTTCCCAA AAGGGAACAC CTGGGGAACC TGGACCTCCT 
GGAGATGATG GATTCCCAGG AGAAAGAGGT GATAAAGGAA CTCCCGGGAT GCAAGGGAGA 
AGAGGAGAGC CGGGAAGATA CGGACCACCT GGATTTCAGA GAGGGGAACC TGGTGAGAAA 
GGTCAGCCAG GGCATCCTGG ACCCCCAGGC CCTCCAGGCT CAACTGGTCT AAGAGGGTTC 
ATTGGTTTTC CAGGACTTCC AGGTGACCAG GGTGAGCCAG GTTCTCCAGG TCCCCCTGGA 
TTTTCAGGAA TTGATGGAGC AAGAGGACCT AAAGGAAACA AAGGTGACCC TGCCAGTCAC 
TTTGGTCCAC CTGGTCCAAA GGGTGAGCCA GGTAGCCCTG GATGTCCAGG GCATTTTGGA 
GCATCTGGAG AGCAGGGCTT GCCTGGTATT CAAGGGCCCA GAGGATCACC TGGAAGGCCA 
GGGCCACCTG GCTCCTCTGG ACCACCAGGG TGCCCAGGTG ATCACGGGAT GCCTGGGCTG 
AGGGGACAGC CAGGAGAAAT GGGAGACCCT GGGCCAAGAG GCCTCCAGGG GGATCCAGGG 
ATACCAGGTC CTCCGGGAAT AAAAGGTCCC TCCGGATCAC CTGGCCTGAA CGGCTTGCAT 
GGATTGAAAG GTCAGAAAGG AACTAAAGGT GCTTCAGGTT TGCATGATGT GGGGCCACCT 
GGTCCAGTGG GAATACCTGG GCTAAAAGGG GAGAGAGGAG ACCCTGGGAG CCCAGGAATC 
TCTCCTCCAG GTCCTCGTGG AGAAAAAGGT CCCCCAGGAC CCCCAGGGAG TTCAGGACCA 
CCTGGTCCTG CAGGTGCCAC AGGAAGAGCT CCTAAGGACA TTCCTGACCC GGGTCCACCT 
GGAGATCAGG GACCTCCTGG TCCTGATGGC CCAAGAGGAG CACCTGGGCC TCCAGGCCTC 
CCTGGGAGTG TTGACCTTCT GAGAGGGGAG CCAGGTGACT GTGGTCTACC AGGGCCACCA 
GGTCCCCCTG GCCCACCAGG CCCTCCAGGA TACAAAGGCT TTCCAGGATG TGATGGAAAA 
GATGGCCAGA AAGGACCAAT GGGATTCCCG GGACCGCAGG GACCACATGG ATTTCCTGGG 
CCACCTGGAG AGAAGGGTTT ACCTGGACCT CCAGGGAGAA AAGGGCCCAC TGGTCTTCCA 
GGTCCCAGAG GTGAACCGGG GCCACCTGCA GATGTGGATG ACTGTCCCCG AATCCCAGGC 
CTTCCTGGGG CGCCAGGCAT GAGAGGACCA GAAGGAGCCA TGGGGCTCTC TGGAATGAGA 
GGCCCCCCAG GACCAGGGTG CAAAGGAGAG CCTGGGCTGG ATGGCAGGAG GGGTGTGGAT 
GGCGTCCCTG GGTCTCCTGG GCCTCCCGGA CGTAAAGGTG ACACAGGAGA AGACGGCTAC 
CCTGGAGGAC CAGGGCCTCC TGGTCCCATT GGGGATCCTG GGCCCAAAGG GTTTGGCCCT 
GGATACCTCG GTGGCTTCCT CCTGGTTCTC CACAGTCAGA CGGACCAGGA GCCCACCTGC 
CCCCTGGGCA TGCCCAGGCT CTGGACTGGG TATAGTCTGT TATACCTGGA AGGGCAAGAG 
AAAGCTCACA ATCAAGACCT TGGTCTGGCA GGGTCTTGCC TTCCCGTGTT TAGCACGCTG 
CCCTTTGCCT ACTGCAACAT CCACCAGGTG TGCCACTATG CCCAGAGAAA CGACAGATCC 
TACTGGCTGG CCAGCGCTGC GCCCCTCCCC ATGATGCCAC TCTCTGAAGA GGCGATCCGC 
CCCTATGTCA GCCGCTGTGC GGTATGCGAG GCCCCGGCCC AGGCGGTGGC GGTGCACAGC 
CAGGACAAGT CCATCCCCCC ATGTCCGCAG ACCTGGAGGA GCCTCTGGAT CGGGTATTCA 
TTCCTGATGC TCATGGGTGT GTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL4A4 ( XM_009444471.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_009442746.2 Pan troglodytes collagen type IV alpha 4 (COL4A4), transcript variant X12, mRNA.

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CloneID	OPc80769
Clone ID Related Accession (Same CDS sequence)	XM_009444471.2
Accession Version	XM_009444471.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4821bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	collagen alpha-4(IV) chain isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006470.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 2, 2016 this sequence version replaced XM_009444471.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGTCTC TGCACATAGT ACTAATGAGG TGCTCCTTCA GATTGACCAA GTCCTTGGCC 
ACAGATCCCT GGTCACTTAC ACTCATTCTC TTTTCTGTAC AATATGTATA TGGGAGTGGA 
AAGAAATACG TTGGTCCTTG TGGAGGAAGA GATTGCTCTG TTTGCCACTG TGTTCCTGAA 
AAGGGGTCTC GGGGTCCACC AGGACCACCA GGGCCACAGG GTCCAATTGG ACCCCTGGGA 
GCCCCAGGAC CCATTGGGCT TTCAGGAGAG AAAGGAATGA GAGGGGACCA CGGCCCTCCT 
GGAGCAGCAG GGGACAAAGG AGATAAGGGT TCAACTGGTG TTCCTGGATT TCCAGGTTTA 
GATGGCATAC CTGGGCACCC AGGGCCTCCT GGACCCAGAG GCAAACCTGG TATGAGTGGC 
CACAATGGCT CAAGAGGTGA CCCAGGGTTT CCAGGAGGAA GAGGAGCTCT TGGCCCAGGA 
GGCCCCCTAG GCCATCCTGG GGAAAAGGGA GAAAAAGGAA ATTCAGTGTT CATTTTAGGT 
GCCGTTAAAG GTATTCAGGG AGACAGAGGG GACCCAGGAC TGCCTGGCTT ACCAGGATCT 
TGGGGGGCAG GAGGACCGGC GGGTCCCACA GGATATCCTG GAGAGCCAGG GTTAGTGGGA 
CCTCCGGGCC AACCAGGGCG TCCAGGTTTG AAGGGAAATC CCGGTGTGGG AGTAAAGGGG 
CAAATGGGAG ACCCGGGTGA GGTTGGTCAG CAAGGTTCTC CTGGACCCAC CCTGTTGGTG 
GAGCCACCTG ACTTTCGTCT CTATAAAGGA GAAAAGGGTA TAAAAGGAAT TCCTGGAATG 
GTTGGACTGC CAGGACCACC AGGACGCAAG GGAGAATCTG ATATTGGGGC AAAAGGAGAA 
AAAGGTATTC CTGGATTTCC AGGGCCTCGG GGGGATCCTG GTTCCTATGG ATCTCCAGGT 
TTTCCAGGAT TAAAGGGAGA ACTAGGACTG GTTGGAGATC CTGGGCTATT TGGATTAATT 
GGCCCAAAGG GGGATCCTGG AAATCGAGGG CACCCAGGAC CACCAGGTGT TTTGGTGACT 
CCACCTCTTC CACTCAAAGG CCCACCAGGG GACCCAGGGT TCCCTGGCCG CTATGGAGAA 
ACAGGGGATG TTGGACCACC TGGTCCCCCA GGTCTCTTGG GCAGACCAGG GGAAGCCTGT 
GCAGGCATGA TAGGACCCCC TGGGCCACAA GGATTTCCTG GTCTTCCTGG GCTTCCAGGA 
GAAGCTGGTA TTCCTGGGAG ACCTGATTCT GCTCCAGGAA AACCAGGGAA GCCAGGATCA 
CCTGGCTTGC CTGGAGCACC AGGCCTGCAG GGCCTCCCAG GATCAAGTGT GATATACTGT 
AGTGTTGGGA ATCCTGGACC ACAAGGAATA AAAGGCAAAG TGGGTCCCCC AGGAGGAAGA 
GGCTCAAAAG GAGAAAAAGG AAATGAAGGA CTCTGTGCCT GTGAGCCTGG ACCCATGGGC 
CCCCCTGGCC CTCCAGGACT TCCTGGGAGG CAGGGGAGTA AGGGAGACTT GGGGCTCCCT 
GGCTGGCTTG GAACAAAAGG TGACCCAGGA CCTCCTGGTG CTGAAGGACC TCCAGGGCTA 
CCAGGAAAGC ATGGTGCCTC TGGACCACCT GGCAACAAAG GGGCGAAGGG TGACATGGTT 
GTATCAAGAG TTAAAGGGCA CAAAGGAGAA AGAGGTCCTG ATGGGCCCCC AGGATTTCCA 
GGGCAGCCAG GATCACATGG TCGGGATGGA CATGCTGGAG AAAAAGGGGA TCCAGGACCC 
CCAGGGGATC ATGAAGATGC GACCCCAGGT GGTAAAGGAT TTCCTGGACC TCTGGGCCCC 
CCGGGCAAAG CAGGACCTTT GGGGCCCCCA GGACTGGGAT TTCCTGGTCC ACCAGGACAG 
CGAGGCCACC CAGGAGTTCC AGGCCACCCA GGTGTGAGGG GCCCTGATGG CTTGAAGGGT 
CAGAAAGGTG ACACAATTTC TTGCAACGTA ACCTACCCTG GGAGGCACGG CCCTCCAGGT 
TTTGATGGAC CTCCAGGTCC GAAGGGATTT CCAGGTCCCC AAGGTGCCCC TGGGCTGAGT 
GGTTCAGATG GGCATAAAGG CAGACCTGGC ACACCAGGAA CAGCGGAAAT ACCAGGTCCA 
CCTGGTTTTC GTGGTGACAT GGGAGATCCG GGTTTTGGAG GTGAAAAGGG GTCCTCCCCT 
GCTGGGCCCC CAGGCCCTCC CGGCTCACCA GGAGTGAATG GTCAGAAAGG AATCCCGGGA 
GACCCTGCAT TTGGTCACCT GGGACCCCCG GGAAAGAGGG GTCTTTCAGG AGTGCCAGGG 
ATAAAAGGAC CCAGAGGTGA TCCGGGATGT CCAGGGGCTG AAGGGCCAGC TGGCATTCCT 
GGATTCCCAG GTCTCAAAGG TCCCAAAGGC AGAGAGGGAC ATGCTGGGTT TCCAGGTGTC 
CCAGGTCCAC CTGGCCATTC CTGTGAAAGA GGTGCTCCAG GGATACCAGG GCAACCGGGA 
CTCCCTGGGT ATCCAGGTAG CCCAGGTGCT CCAGGTGGGA AAGGACAGCT GGGAGATGTG 
GGGCCTCCCG GGCCAGCTGG AATGAAAGGC CTCCCTGGAC TCCCAGGATG GCCTGGGGCA 
CATGGTCCCC CAGGCCTCCC AGGAATCCCA GGTCCCTTTG GAGATGATGG GCTACCTGGT 
CCTCCAGGTC CAAAGGGACC CCAGGGGCTG CCTGGTTTCC CAGGTTTTCC CGGAGAAAGA 
GGAAAGCCTG GTGCAGAGGG ATGTCCTGGC ACAAAGGGAG AACCTGGAGA GAAGGGCATG 
TCTGGCCTTC CCGGAGACCG GGGACTGAGA GGGGCCAAAG GAGCCATAGG ACCTCCCGGA 
GATGAAGGAG AAATGGCTAT CATTTCCCAA AAGGGAACAC CTGGGGAACC TGGACCTCCT 
GGAGATGATG GATTCCCAGG AGAAAGAGGT GATAAAGGAA CTCCCGGGAT GCAAGGGAGA 
AGAGGAGAGC CGGGAAGATA CGGACCACCT GGATTTCAGA GAGGGGAACC TGGTGAGAAA 
GGTCAGCCAG GGCATCCTGG ACCCCCAGGC CCTCCAGGCT CAACTGGTCT AAGAGGGTTC 
ATTGGTTTTC CAGGACTTCC AGGTGACCAG GGTGAGCCAG GTTCTCCAGG TCCCCCTGGA 
TTTTCAGGAA TTGATGGAGC AAGAGGACCT AAAGGAAACA AAGGTGACCC TGCCAGTCAC 
TTTGGTCCAC CTGGTCCAAA GGGTGAGCCA GGTAGCCCTG GATGTCCAGG GCATTTTGGA 
GCATCTGGAG AGCAGGGCTT GCCTGGTATT CAAGGGCCCA GAGGATCACC TGGAAGGCCA 
GGGCCACCTG GCTCCTCTGG ACCACCAGGG TGCCCAGGTG ATCACGGGAT GCCTGGGCTG 
AGGGGACAGC CAGGAGAAAT GGGAGACCCT GGGCCAAGAG GCCTCCAGGG GGATCCAGGG 
ATACCAGGTC CTCCGGGAAT AAAAGGTCCC TCCGGATCAC CTGGCCTGAA CGGCTTGCAT 
GGATTGAAAG GTCAGAAAGG AACTAAAGGT GCTTCAGGTT TGCATGATGT GGGGCCACCT 
GGTCCAGTGG GAATACCTGG GCTAAAAGGG GAGAGAGGAG ACCCTGGGAG CCCAGGAATC 
TCTCCTCCAG GTCCTCGTGG AGAAAAAGGT CCCCCAGGAC CCCCAGGGAG TTCAGGACCA 
CCTGGTCCTG CAGGTGCCAC AGGAAGAGCT CCTAAGGACA TTCCTGACCC GGGTCCACCT 
GGAGATCAGG GACCTCCTGG TCCTGATGGC CCAAGAGGAG CACCTGGGCC TCCAGGCCTC 
CCTGGGAGTG TTGACCTTCT GAGAGGGGAG CCAGGTGACT GTGGTCTACC AGGGCCACCA 
GGTCCCCCTG GCCCACCAGG CCCTCCAGGA TACAAAGGCT TTCCAGGATG TGATGGAAAA 
GATGGCCAGA AAGGACCAAT GGGATTCCCG GGACCGCAGG GACCACATGG ATTTCCTGGG 
CCACCTGGAG AGAAGGGTTT ACCTGGACCT CCAGGGAGAA AAGGGCCCAC TGGTCTTCCA 
GGTCCCAGAG GTGAACCGGG GCCACCTGCA GATGTGGATG ACTGTCCCCG AATCCCAGGC 
CTTCCTGGGG CGCCAGGCAT GAGAGGACCA GAAGGAGCCA TGGGGCTCTC TGGAATGAGA 
GGCCCCCCAG GACCAGGGTG CAAAGGAGAG CCTGGGCTGG ATGGCAGGAG GGGTGTGGAT 
GGCGTCCCTG GGTCTCCTGG GCCTCCCGGA CGTAAAGGTG ACACAGGAGA AGACGGCTAC 
CCTGGAGGAC CAGGGCCTCC TGGTCCCATT GGGGATCCTG GGCCCAAAGG GTTTGGCCCT 
GGATACCTCG GTGGCTTCCT CCTGGTTCTC CACAGTCAGA CGGACCAGGA GCCCACCTGC 
CCCCTGGGCA TGCCCAGGCT CTGGACTGGG TATAGTCTGT TATACCTGGA AGGGCAAGAG 
AAAGCTCACA ATCAAGACCT TGGTCTGGCA GGGTCTTGCC TTCCCGTGTT TAGCACGCTG 
CCCTTTGCCT ACTGCAACAT CCACCAGGTG TGCCACTATG CCCAGAGAAA CGACAGATCC 
TACTGGCTGG CCAGCGCTGC GCCCCTCCCC ATGATGCCAC TCTCTGAAGA GGCGATCCGC 
CCCTATGTCA GCCGCTGTGC GGTATGCGAG GCCCCGGCCC AGGCGGTGGC GGTGCACAGC 
CAGGACAAGT CCATCCCCCC ATGTCCGCAG ACCTGGAGGA GCCTCTGGAT CGGGTATTCA 
TTCCTGATGC TTACATTGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009442746.2
CDS	424..5244
Translation	MWSLHIVLMRCSFRLTKSLATDPWSLTLILFSVQYVYGSGKKYVGPCGGRDCSVCHCVPEKGSRGPPGPPGPQGPIGPLGAPGPIGLSGEKGMRGDHGPPGAAGDKGDKGSTGVPGFPGLDGIPGHPGPPGPRGKPGMSGHNGSRGDPGFPGGRGALGPGGPLGHPGEKGEKGNSVFILGAVKGIQGDRGDPGLPGLPGSWGAGGPAGPTGYPGEPGLVGPPGQPGRPGLKGNPGVGVKGQMGDPGEVGQQGSPGPTLLVEPPDFRLYKGEKGIKGIPGMVGLPGPPGRKGESDIGAKGEKGIPGFPGPRGDPGSYGSPGFPGLKGELGLVGDPGLFGLIGPKGDPGNRGHPGPPGVLVTPPLPLKGPPGDPGFPGRYGETGDVGPPGPPGLLGRPGEACAGMIGPPGPQGFPGLPGLPGEAGIPGRPDSAPGKPGKPGSPGLPGAPGLQGLPGSSVIYCSVGNPGPQGIKGKVGPPGGRGSKGEKGNEGLCACEPGPMGPPGPPGLPGRQGSKGDLGLPGWLGTKGDPGPPGAEGPPGLPGKHGASGPPGNKGAKGDMVVSRVKGHKGERGPDGPPGFPGQPGSHGRDGHAGEKGDPGPPGDHEDATPGGKGFPGPLGPPGKAGPLGPPGLGFPGPPGQRGHPGVPGHPGVRGPDGLKGQKGDTISCNVTYPGRHGPPGFDGPPGPKGFPGPQGAPGLSGSDGHKGRPGTPGTAEIPGPPGFRGDMGDPGFGGEKGSSPAGPPGPPGSPGVNGQKGIPGDPAFGHLGPPGKRGLSGVPGIKGPRGDPGCPGAEGPAGIPGFPGLKGPKGREGHAGFPGVPGPPGHSCERGAPGIPGQPGLPGYPGSPGAPGGKGQLGDVGPPGPAGMKGLPGLPGWPGAHGPPGLPGIPGPFGDDGLPGPPGPKGPQGLPGFPGFPGERGKPGAEGCPGTKGEPGEKGMSGLPGDRGLRGAKGAIGPPGDEGEMAIISQKGTPGEPGPPGDDGFPGERGDKGTPGMQGRRGEPGRYGPPGFQRGEPGEKGQPGHPGPPGPPGSTGLRGFIGFPGLPGDQGEPGSPGPPGFSGIDGARGPKGNKGDPASHFGPPGPKGEPGSPGCPGHFGASGEQGLPGIQGPRGSPGRPGPPGSSGPPGCPGDHGMPGLRGQPGEMGDPGPRGLQGDPGIPGPPGIKGPSGSPGLNGLHGLKGQKGTKGASGLHDVGPPGPVGIPGLKGERGDPGSPGISPPGPRGEKGPPGPPGSSGPPGPAGATGRAPKDIPDPGPPGDQGPPGPDGPRGAPGPPGLPGSVDLLRGEPGDCGLPGPPGPPGPPGPPGYKGFPGCDGKDGQKGPMGFPGPQGPHGFPGPPGEKGLPGPPGRKGPTGLPGPRGEPGPPADVDDCPRIPGLPGAPGMRGPEGAMGLSGMRGPPGPGCKGEPGLDGRRGVDGVPGSPGPPGRKGDTGEDGYPGGPGPPGPIGDPGPKGFGPGYLGGFLLVLHSQTDQEPTCPLGMPRLWTGYSLLYLEGQEKAHNQDLGLAGSCLPVFSTLPFAYCNIHQVCHYAQRNDRSYWLASAAPLPMMPLSEEAIRPYVSRCAVCEAPAQAVAVHSQDKSIPPCPQTWRSLWIGYSFLMLTL

Target ORF information:

RefSeq Version	XM_009444471.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes collagen type IV alpha 4 (COL4A4), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009444471.2

ORF Insert Sequence:

ATGTGGTCTC TGCACATAGT ACTAATGAGG TGCTCCTTCA GATTGACCAA GTCCTTGGCC 
ACAGATCCCT GGTCACTTAC ACTCATTCTC TTTTCTGTAC AATATGTATA TGGGAGTGGA 
AAGAAATACG TTGGTCCTTG TGGAGGAAGA GATTGCTCTG TTTGCCACTG TGTTCCTGAA 
AAGGGGTCTC GGGGTCCACC AGGACCACCA GGGCCACAGG GTCCAATTGG ACCCCTGGGA 
GCCCCAGGAC CCATTGGGCT TTCAGGAGAG AAAGGAATGA GAGGGGACCA CGGCCCTCCT 
GGAGCAGCAG GGGACAAAGG AGATAAGGGT TCAACTGGTG TTCCTGGATT TCCAGGTTTA 
GATGGCATAC CTGGGCACCC AGGGCCTCCT GGACCCAGAG GCAAACCTGG TATGAGTGGC 
CACAATGGCT CAAGAGGTGA CCCAGGGTTT CCAGGAGGAA GAGGAGCTCT TGGCCCAGGA 
GGCCCCCTAG GCCATCCTGG GGAAAAGGGA GAAAAAGGAA ATTCAGTGTT CATTTTAGGT 
GCCGTTAAAG GTATTCAGGG AGACAGAGGG GACCCAGGAC TGCCTGGCTT ACCAGGATCT 
TGGGGGGCAG GAGGACCGGC GGGTCCCACA GGATATCCTG GAGAGCCAGG GTTAGTGGGA 
CCTCCGGGCC AACCAGGGCG TCCAGGTTTG AAGGGAAATC CCGGTGTGGG AGTAAAGGGG 
CAAATGGGAG ACCCGGGTGA GGTTGGTCAG CAAGGTTCTC CTGGACCCAC CCTGTTGGTG 
GAGCCACCTG ACTTTCGTCT CTATAAAGGA GAAAAGGGTA TAAAAGGAAT TCCTGGAATG 
GTTGGACTGC CAGGACCACC AGGACGCAAG GGAGAATCTG ATATTGGGGC AAAAGGAGAA 
AAAGGTATTC CTGGATTTCC AGGGCCTCGG GGGGATCCTG GTTCCTATGG ATCTCCAGGT 
TTTCCAGGAT TAAAGGGAGA ACTAGGACTG GTTGGAGATC CTGGGCTATT TGGATTAATT 
GGCCCAAAGG GGGATCCTGG AAATCGAGGG CACCCAGGAC CACCAGGTGT TTTGGTGACT 
CCACCTCTTC CACTCAAAGG CCCACCAGGG GACCCAGGGT TCCCTGGCCG CTATGGAGAA 
ACAGGGGATG TTGGACCACC TGGTCCCCCA GGTCTCTTGG GCAGACCAGG GGAAGCCTGT 
GCAGGCATGA TAGGACCCCC TGGGCCACAA GGATTTCCTG GTCTTCCTGG GCTTCCAGGA 
GAAGCTGGTA TTCCTGGGAG ACCTGATTCT GCTCCAGGAA AACCAGGGAA GCCAGGATCA 
CCTGGCTTGC CTGGAGCACC AGGCCTGCAG GGCCTCCCAG GATCAAGTGT GATATACTGT 
AGTGTTGGGA ATCCTGGACC ACAAGGAATA AAAGGCAAAG TGGGTCCCCC AGGAGGAAGA 
GGCTCAAAAG GAGAAAAAGG AAATGAAGGA CTCTGTGCCT GTGAGCCTGG ACCCATGGGC 
CCCCCTGGCC CTCCAGGACT TCCTGGGAGG CAGGGGAGTA AGGGAGACTT GGGGCTCCCT 
GGCTGGCTTG GAACAAAAGG TGACCCAGGA CCTCCTGGTG CTGAAGGACC TCCAGGGCTA 
CCAGGAAAGC ATGGTGCCTC TGGACCACCT GGCAACAAAG GGGCGAAGGG TGACATGGTT 
GTATCAAGAG TTAAAGGGCA CAAAGGAGAA AGAGGTCCTG ATGGGCCCCC AGGATTTCCA 
GGGCAGCCAG GATCACATGG TCGGGATGGA CATGCTGGAG AAAAAGGGGA TCCAGGACCC 
CCAGGGGATC ATGAAGATGC GACCCCAGGT GGTAAAGGAT TTCCTGGACC TCTGGGCCCC 
CCGGGCAAAG CAGGACCTTT GGGGCCCCCA GGACTGGGAT TTCCTGGTCC ACCAGGACAG 
CGAGGCCACC CAGGAGTTCC AGGCCACCCA GGTGTGAGGG GCCCTGATGG CTTGAAGGGT 
CAGAAAGGTG ACACAATTTC TTGCAACGTA ACCTACCCTG GGAGGCACGG CCCTCCAGGT 
TTTGATGGAC CTCCAGGTCC GAAGGGATTT CCAGGTCCCC AAGGTGCCCC TGGGCTGAGT 
GGTTCAGATG GGCATAAAGG CAGACCTGGC ACACCAGGAA CAGCGGAAAT ACCAGGTCCA 
CCTGGTTTTC GTGGTGACAT GGGAGATCCG GGTTTTGGAG GTGAAAAGGG GTCCTCCCCT 
GCTGGGCCCC CAGGCCCTCC CGGCTCACCA GGAGTGAATG GTCAGAAAGG AATCCCGGGA 
GACCCTGCAT TTGGTCACCT GGGACCCCCG GGAAAGAGGG GTCTTTCAGG AGTGCCAGGG 
ATAAAAGGAC CCAGAGGTGA TCCGGGATGT CCAGGGGCTG AAGGGCCAGC TGGCATTCCT 
GGATTCCCAG GTCTCAAAGG TCCCAAAGGC AGAGAGGGAC ATGCTGGGTT TCCAGGTGTC 
CCAGGTCCAC CTGGCCATTC CTGTGAAAGA GGTGCTCCAG GGATACCAGG GCAACCGGGA 
CTCCCTGGGT ATCCAGGTAG CCCAGGTGCT CCAGGTGGGA AAGGACAGCT GGGAGATGTG 
GGGCCTCCCG GGCCAGCTGG AATGAAAGGC CTCCCTGGAC TCCCAGGATG GCCTGGGGCA 
CATGGTCCCC CAGGCCTCCC AGGAATCCCA GGTCCCTTTG GAGATGATGG GCTACCTGGT 
CCTCCAGGTC CAAAGGGACC CCAGGGGCTG CCTGGTTTCC CAGGTTTTCC CGGAGAAAGA 
GGAAAGCCTG GTGCAGAGGG ATGTCCTGGC ACAAAGGGAG AACCTGGAGA GAAGGGCATG 
TCTGGCCTTC CCGGAGACCG GGGACTGAGA GGGGCCAAAG GAGCCATAGG ACCTCCCGGA 
GATGAAGGAG AAATGGCTAT CATTTCCCAA AAGGGAACAC CTGGGGAACC TGGACCTCCT 
GGAGATGATG GATTCCCAGG AGAAAGAGGT GATAAAGGAA CTCCCGGGAT GCAAGGGAGA 
AGAGGAGAGC CGGGAAGATA CGGACCACCT GGATTTCAGA GAGGGGAACC TGGTGAGAAA 
GGTCAGCCAG GGCATCCTGG ACCCCCAGGC CCTCCAGGCT CAACTGGTCT AAGAGGGTTC 
ATTGGTTTTC CAGGACTTCC AGGTGACCAG GGTGAGCCAG GTTCTCCAGG TCCCCCTGGA 
TTTTCAGGAA TTGATGGAGC AAGAGGACCT AAAGGAAACA AAGGTGACCC TGCCAGTCAC 
TTTGGTCCAC CTGGTCCAAA GGGTGAGCCA GGTAGCCCTG GATGTCCAGG GCATTTTGGA 
GCATCTGGAG AGCAGGGCTT GCCTGGTATT CAAGGGCCCA GAGGATCACC TGGAAGGCCA 
GGGCCACCTG GCTCCTCTGG ACCACCAGGG TGCCCAGGTG ATCACGGGAT GCCTGGGCTG 
AGGGGACAGC CAGGAGAAAT GGGAGACCCT GGGCCAAGAG GCCTCCAGGG GGATCCAGGG 
ATACCAGGTC CTCCGGGAAT AAAAGGTCCC TCCGGATCAC CTGGCCTGAA CGGCTTGCAT 
GGATTGAAAG GTCAGAAAGG AACTAAAGGT GCTTCAGGTT TGCATGATGT GGGGCCACCT 
GGTCCAGTGG GAATACCTGG GCTAAAAGGG GAGAGAGGAG ACCCTGGGAG CCCAGGAATC 
TCTCCTCCAG GTCCTCGTGG AGAAAAAGGT CCCCCAGGAC CCCCAGGGAG TTCAGGACCA 
CCTGGTCCTG CAGGTGCCAC AGGAAGAGCT CCTAAGGACA TTCCTGACCC GGGTCCACCT 
GGAGATCAGG GACCTCCTGG TCCTGATGGC CCAAGAGGAG CACCTGGGCC TCCAGGCCTC 
CCTGGGAGTG TTGACCTTCT GAGAGGGGAG CCAGGTGACT GTGGTCTACC AGGGCCACCA 
GGTCCCCCTG GCCCACCAGG CCCTCCAGGA TACAAAGGCT TTCCAGGATG TGATGGAAAA 
GATGGCCAGA AAGGACCAAT GGGATTCCCG GGACCGCAGG GACCACATGG ATTTCCTGGG 
CCACCTGGAG AGAAGGGTTT ACCTGGACCT CCAGGGAGAA AAGGGCCCAC TGGTCTTCCA 
GGTCCCAGAG GTGAACCGGG GCCACCTGCA GATGTGGATG ACTGTCCCCG AATCCCAGGC 
CTTCCTGGGG CGCCAGGCAT GAGAGGACCA GAAGGAGCCA TGGGGCTCTC TGGAATGAGA 
GGCCCCCCAG GACCAGGGTG CAAAGGAGAG CCTGGGCTGG ATGGCAGGAG GGGTGTGGAT 
GGCGTCCCTG GGTCTCCTGG GCCTCCCGGA CGTAAAGGTG ACACAGGAGA AGACGGCTAC 
CCTGGAGGAC CAGGGCCTCC TGGTCCCATT GGGGATCCTG GGCCCAAAGG GTTTGGCCCT 
GGATACCTCG GTGGCTTCCT CCTGGTTCTC CACAGTCAGA CGGACCAGGA GCCCACCTGC 
CCCCTGGGCA TGCCCAGGCT CTGGACTGGG TATAGTCTGT TATACCTGGA AGGGCAAGAG 
AAAGCTCACA ATCAAGACCT TGGTCTGGCA GGGTCTTGCC TTCCCGTGTT TAGCACGCTG 
CCCTTTGCCT ACTGCAACAT CCACCAGGTG TGCCACTATG CCCAGAGAAA CGACAGATCC 
TACTGGCTGG CCAGCGCTGC GCCCCTCCCC ATGATGCCAC TCTCTGAAGA GGCGATCCGC 
CCCTATGTCA GCCGCTGTGC GGTATGCGAG GCCCCGGCCC AGGCGGTGGC GGTGCACAGC 
CAGGACAAGT CCATCCCCCC ATGTCCGCAG ACCTGGAGGA GCCTCTGGAT CGGGTATTCA 
TTCCTGATGC TTACATTGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL4A4 ( XM_016950595.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016806084.1 Pan troglodytes collagen type IV alpha 4 (COL4A4), transcript variant X15, mRNA.

Price & Availability↑

CloneID	OPc80770
Clone ID Related Accession (Same CDS sequence)	XM_016950595.1 , XM_009444472.2 , XM_016950594.1 , XM_016950596.1
Accession Version	XM_016950595.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4245bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	collagen alpha-4(IV) chain isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006470.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGTCTC TGCACATAGT ACTAATGAGG TGCTCCTTCA GATTGACCAA GTCCTTGGCC 
ACAGATCCCT GGTCACTTAC ACTCATTCTC TTTTCTGTAC AATATGTATA TGGGAGTGGA 
AAGAAATACG TTGGTCCTTG TGGAGGAAGA GATTGCTCTG TTTGCCACTG TGTTCCTGAA 
AAGGGGTCTC GGGGTCCACC AGGACCACCA GGGCCACAGG GTCCAATTGG ACCCCTGGGA 
GCCCCAGGAC CCATTGGGCT TTCAGGAGAG AAAGGAATGA GAGGGGACCA CGGCCCTCCT 
GGAGCAGCAG GGGACAAAGG AGATAAGGGT TCAACTGGTG TTCCTGGATT TCCAGGTTTA 
GATGGCATAC CTGGGCACCC AGGGCCTCCT GGACCCAGAG GCAAACCTGG TATGAGTGGC 
CACAATGGCT CAAGAGGTGA CCCAGGGTTT CCAGGAGGAA GAGGAGCTCT TGGCCCAGGA 
GGCCCCCTAG GCCATCCTGG GGAAAAGGGA GAAAAAGGAA ATTCAGTGTT CATTTTAGGT 
GCCGTTAAAG GTATTCAGGG AGACAGAGGG GACCCAGGAC TGCCTGGCTT ACCAGGATCT 
TGGGGGGCAG GAGGACCGGC GGGTCCCACA GGATATCCTG GAGAGCCAGG GTTAGTGGGA 
CCTCCGGGCC AACCAGGGCG TCCAGGTTTG AAGGGAAATC CCGGTGTGGG AGTAAAGGGG 
CAAATGGGAG ACCCGGGTGA GGTTGGTCAG CAAGGTTCTC CTGGACCCAC CCTGTTGGTG 
GAGCCACCTG ACTTTCGTCT CTATAAAGGA GAAAAGGGTA TAAAAGGAAT TCCTGGAATG 
GTTGGACTGC CAGGACCACC AGGACGCAAG GGAGAATCTG ATATTGGGGC AAAAGGAGAA 
AAAGGTATTC CTGGATTTCC AGGGCCTCGG GGGGATCCTG GTTCCTATGG ATCTCCAGGT 
TTTCCAGGAT TAAAGGGAGA ACTAGGACTG GTTGGAGATC CTGGGCTATT TGGATTAATT 
GGCCCAAAGG GGGATCCTGG AAATCGAGGG CACCCAGGAC CACCAGGTGT TTTGGTGACT 
CCACCTCTTC CACTCAAAGG CCCACCAGGG GACCCAGGGT TCCCTGGCCG CTATGGAGAA 
ACAGGGGATG TTGGACCACC TGGTCCCCCA GGTCTCTTGG GCAGACCAGG GGAAGCCTGT 
GCAGGCATGA TAGGACCCCC TGGGCCACAA GGATTTCCTG GTCTTCCTGG GCTTCCAGGA 
GAAGCTGGTA TTCCTGGGAG ACCTGATTCT GCTCCAGGAA AACCAGGGAA GCCAGGATCA 
CCTGGCTTGC CTGGAGCACC AGGCCTGCAG GGCCTCCCAG GATCAAGTGT GATATACTGT 
AGTGTTGGGA ATCCTGGACC ACAAGGAATA AAAGGCAAAG TGGGTCCCCC AGGAGGAAGA 
GGCTCAAAAG GAGAAAAAGG AAATGAAGGA CTCTGTGCCT GTGAGCCTGG ACCCATGGGC 
CCCCCTGGCC CTCCAGGACT TCCTGGGAGG CAGGGGAGTA AGGGAGACTT GGGGCTCCCT 
GGCTGGCTTG GAACAAAAGG TGACCCAGGA CCTCCTGGTG CTGAAGGACC TCCAGGGCTA 
CCAGGAAAGC ATGGTGCCTC TGGACCACCT GGCAACAAAG GGGCGAAGGG TGACATGGTT 
GTATCAAGAG TTAAAGGGCA CAAAGGAGAA AGAGGTCCTG ATGGGCCCCC AGGATTTCCA 
GGGCAGCCAG GATCACATGG TCGGGATGGA CATGCTGGAG AAAAAGGGGA TCCAGGACCC 
CCAGGGGATC ATGAAGATGC GACCCCAGGT GGTAAAGGAT TTCCTGGACC TCTGGGCCCC 
CCGGGCAAAG CAGGACCTTT GGGGCCCCCA GGACTGGGAT TTCCTGGTCC ACCAGGACAG 
CGAGGCCACC CAGGAGTTCC AGGCCACCCA GGTGTGAGGG GCCCTGATGG CTTGAAGGGT 
CAGAAAGGTG ACACAATTTC TTGCAACGTA ACCTACCCTG GGAGGCACGG CCCTCCAGGT 
TTTGATGGAC CTCCAGGTCC GAAGGGATTT CCAGGTCCCC AAGGTGCCCC TGGGCTGAGT 
GGTTCAGATG GGCATAAAGG CAGACCTGGC ACACCAGGAA CAGCGGAAAT ACCAGGTCCA 
CCTGGTTTTC GTGGTGACAT GGGAGATCCG GGTTTTGGAG GTGAAAAGGG GTCCTCCCCT 
GCTGGGCCCC CAGGCCCTCC CGGCTCACCA GGAGTGAATG GTCAGAAAGG AATCCCGGGA 
GACCCTGCAT TTGGTCACCT GGGACCCCCG GGAAAGAGGG GTCTTTCAGG AGTGCCAGGG 
ATAAAAGGAC CCAGAGGTGA TCCGGGATGT CCAGGGGCTG AAGGGCCAGC TGGCATTCCT 
GGATTCCCAG GTCTCAAAGG TCCCAAAGGC AGAGAGGGAC ATGCTGGGTT TCCAGGTGTC 
CCAGGTCCAC CTGGCCATTC CTGTGAAAGA GGTGCTCCAG GGATACCAGG GCAACCGGGA 
CTCCCTGGGT ATCCAGGTAG CCCAGGTGCT CCAGGTGGGA AAGGACAGCT GGGAGATGTG 
GGGCCTCCCG GGCCAGCTGG AATGAAAGGC CTCCCTGGAC TCCCAGGATG GCCTGGGGCA 
CATGGTCCCC CAGGCCTCCC AGGAATCCCA GGTCCCTTTG GAGATGATGG GCTACCTGGT 
CCTCCAGGTC CAAAGGGACC CCAGGGGCTG CCTGGTTTCC CAGGTTTTCC CGGAGAAAGA 
GGAAAGCCTG GTGCAGAGGG ATGTCCTGGC ACAAAGGGAG AACCTGGAGA GAAGGGCATG 
TCTGGCCTTC CCGGAGACCG GGGACTGAGA GGGGCCAAAG GAGCCATAGG ACCTCCCGGA 
GATGAAGGAG AAATGGCTAT CATTTCCCAA AAGGGAACAC CTGGGGAACC TGGACCTCCT 
GGAGATGATG GATTCCCAGG AGAAAGAGGT GATAAAGGAA CTCCCGGGAT GCAAGGGAGA 
AGAGGAGAGC CGGGAAGATA CGGACCACCT GGATTTCAGA GAGGGGAACC TGGTGAGAAA 
GGTCAGCCAG GGCATCCTGG ACCCCCAGGC CCTCCAGGCT CAACTGGTCT AAGAGGGTTC 
ATTGGTTTTC CAGGACTTCC AGGTGACCAG GGTGAGCCAG GTTCTCCAGG TCCCCCTGGA 
TTTTCAGGAA TTGATGGAGC AAGAGGACCT AAAGGAAACA AAGGTGACCC TGCCAGTCAC 
TTTGGTCCAC CTGGTCCAAA GGGTGAGCCA GGTAGCCCTG GATGTCCAGG GCATTTTGGA 
GCATCTGGAG AGCAGGGCTT GCCTGGTATT CAAGGGCCCA GAGGATCACC TGGAAGGCCA 
GGGCCACCTG GCTCCTCTGG ACCACCAGGG TGCCCAGGTG ATCACGGGAT GCCTGGGCTG 
AGGGGACAGC CAGGAGAAAT GGGAGACCCT GGGCCAAGAG GCCTCCAGGG GGATCCAGGG 
ATACCAGGTC CTCCGGGAAT AAAAGGTCCC TCCGGATCAC CTGGCCTGAA CGGCTTGCAT 
GGATTGAAAG GTCAGAAAGG AACTAAAGGT GCTTCAGGTT TGCATGATGT GGGGCCACCT 
GGTCCAGTGG GAATACCTGG GCTAAAAGGG GAGAGAGGAG ACCCTGGGAG CCCAGGAATC 
TCTCCTCCAG GTCCTCGTGG AGAAAAAGGT CCCCCAGGAC CCCCAGGGAG TTCAGGACCA 
CCTGGTCCTG CAGGTGCCAC AGGAAGAGCT CCTAAGGACA TTCCTGACCC GGGTCCACCT 
GGAGATCAGG GACCTCCTGG TCCTGATGGC CCAAGAGGAG CACCTGGGCC TCCAGGCCTC 
CCTGGGAGTG TTGACCTTCT GAGAGGGGAG CCAGGTGACT GTGGTCTACC AGGGCCACCA 
GGTCCCCCTG GCCCACCAGG CCCTCCAGGA TACAAAGGCT TTCCAGGATG TGATGGAAAA 
GATGGCCAGA AAGGACCAAT GGGATTCCCG GGACCGCAGG GACCACATGG ATTTCCTGGG 
CCACCTGGAG AGAAGGGTTT ACCTGGACCT CCAGGGAGAA AAGGGCCCAC TGGTCTTCCA 
GGTCCCAGAG GTGAACCGGG GCCACCTGCA GATGTGGATG ACTGTCCCCG AATCCCAGGC 
CTTCCTGGGG CGCCAGGCAT GAGAGGACCA GAAGGAGCCA TGGGGCTCTC TGGAATGAGA 
GGCCCCCCAG GACCAGCTCA TGGGTGTGTT CTGAGGACCA AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016806084.1
CDS	423..4667
Translation	MWSLHIVLMRCSFRLTKSLATDPWSLTLILFSVQYVYGSGKKYVGPCGGRDCSVCHCVPEKGSRGPPGPPGPQGPIGPLGAPGPIGLSGEKGMRGDHGPPGAAGDKGDKGSTGVPGFPGLDGIPGHPGPPGPRGKPGMSGHNGSRGDPGFPGGRGALGPGGPLGHPGEKGEKGNSVFILGAVKGIQGDRGDPGLPGLPGSWGAGGPAGPTGYPGEPGLVGPPGQPGRPGLKGNPGVGVKGQMGDPGEVGQQGSPGPTLLVEPPDFRLYKGEKGIKGIPGMVGLPGPPGRKGESDIGAKGEKGIPGFPGPRGDPGSYGSPGFPGLKGELGLVGDPGLFGLIGPKGDPGNRGHPGPPGVLVTPPLPLKGPPGDPGFPGRYGETGDVGPPGPPGLLGRPGEACAGMIGPPGPQGFPGLPGLPGEAGIPGRPDSAPGKPGKPGSPGLPGAPGLQGLPGSSVIYCSVGNPGPQGIKGKVGPPGGRGSKGEKGNEGLCACEPGPMGPPGPPGLPGRQGSKGDLGLPGWLGTKGDPGPPGAEGPPGLPGKHGASGPPGNKGAKGDMVVSRVKGHKGERGPDGPPGFPGQPGSHGRDGHAGEKGDPGPPGDHEDATPGGKGFPGPLGPPGKAGPLGPPGLGFPGPPGQRGHPGVPGHPGVRGPDGLKGQKGDTISCNVTYPGRHGPPGFDGPPGPKGFPGPQGAPGLSGSDGHKGRPGTPGTAEIPGPPGFRGDMGDPGFGGEKGSSPAGPPGPPGSPGVNGQKGIPGDPAFGHLGPPGKRGLSGVPGIKGPRGDPGCPGAEGPAGIPGFPGLKGPKGREGHAGFPGVPGPPGHSCERGAPGIPGQPGLPGYPGSPGAPGGKGQLGDVGPPGPAGMKGLPGLPGWPGAHGPPGLPGIPGPFGDDGLPGPPGPKGPQGLPGFPGFPGERGKPGAEGCPGTKGEPGEKGMSGLPGDRGLRGAKGAIGPPGDEGEMAIISQKGTPGEPGPPGDDGFPGERGDKGTPGMQGRRGEPGRYGPPGFQRGEPGEKGQPGHPGPPGPPGSTGLRGFIGFPGLPGDQGEPGSPGPPGFSGIDGARGPKGNKGDPASHFGPPGPKGEPGSPGCPGHFGASGEQGLPGIQGPRGSPGRPGPPGSSGPPGCPGDHGMPGLRGQPGEMGDPGPRGLQGDPGIPGPPGIKGPSGSPGLNGLHGLKGQKGTKGASGLHDVGPPGPVGIPGLKGERGDPGSPGISPPGPRGEKGPPGPPGSSGPPGPAGATGRAPKDIPDPGPPGDQGPPGPDGPRGAPGPPGLPGSVDLLRGEPGDCGLPGPPGPPGPPGPPGYKGFPGCDGKDGQKGPMGFPGPQGPHGFPGPPGEKGLPGPPGRKGPTGLPGPRGEPGPPADVDDCPRIPGLPGAPGMRGPEGAMGLSGMRGPPGPAHGCVLRTK

Target ORF information:

RefSeq Version	XM_016950595.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes collagen type IV alpha 4 (COL4A4), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016950595.1

ORF Insert Sequence:

ATGTGGTCTC TGCACATAGT ACTAATGAGG TGCTCCTTCA GATTGACCAA GTCCTTGGCC 
ACAGATCCCT GGTCACTTAC ACTCATTCTC TTTTCTGTAC AATATGTATA TGGGAGTGGA 
AAGAAATACG TTGGTCCTTG TGGAGGAAGA GATTGCTCTG TTTGCCACTG TGTTCCTGAA 
AAGGGGTCTC GGGGTCCACC AGGACCACCA GGGCCACAGG GTCCAATTGG ACCCCTGGGA 
GCCCCAGGAC CCATTGGGCT TTCAGGAGAG AAAGGAATGA GAGGGGACCA CGGCCCTCCT 
GGAGCAGCAG GGGACAAAGG AGATAAGGGT TCAACTGGTG TTCCTGGATT TCCAGGTTTA 
GATGGCATAC CTGGGCACCC AGGGCCTCCT GGACCCAGAG GCAAACCTGG TATGAGTGGC 
CACAATGGCT CAAGAGGTGA CCCAGGGTTT CCAGGAGGAA GAGGAGCTCT TGGCCCAGGA 
GGCCCCCTAG GCCATCCTGG GGAAAAGGGA GAAAAAGGAA ATTCAGTGTT CATTTTAGGT 
GCCGTTAAAG GTATTCAGGG AGACAGAGGG GACCCAGGAC TGCCTGGCTT ACCAGGATCT 
TGGGGGGCAG GAGGACCGGC GGGTCCCACA GGATATCCTG GAGAGCCAGG GTTAGTGGGA 
CCTCCGGGCC AACCAGGGCG TCCAGGTTTG AAGGGAAATC CCGGTGTGGG AGTAAAGGGG 
CAAATGGGAG ACCCGGGTGA GGTTGGTCAG CAAGGTTCTC CTGGACCCAC CCTGTTGGTG 
GAGCCACCTG ACTTTCGTCT CTATAAAGGA GAAAAGGGTA TAAAAGGAAT TCCTGGAATG 
GTTGGACTGC CAGGACCACC AGGACGCAAG GGAGAATCTG ATATTGGGGC AAAAGGAGAA 
AAAGGTATTC CTGGATTTCC AGGGCCTCGG GGGGATCCTG GTTCCTATGG ATCTCCAGGT 
TTTCCAGGAT TAAAGGGAGA ACTAGGACTG GTTGGAGATC CTGGGCTATT TGGATTAATT 
GGCCCAAAGG GGGATCCTGG AAATCGAGGG CACCCAGGAC CACCAGGTGT TTTGGTGACT 
CCACCTCTTC CACTCAAAGG CCCACCAGGG GACCCAGGGT TCCCTGGCCG CTATGGAGAA 
ACAGGGGATG TTGGACCACC TGGTCCCCCA GGTCTCTTGG GCAGACCAGG GGAAGCCTGT 
GCAGGCATGA TAGGACCCCC TGGGCCACAA GGATTTCCTG GTCTTCCTGG GCTTCCAGGA 
GAAGCTGGTA TTCCTGGGAG ACCTGATTCT GCTCCAGGAA AACCAGGGAA GCCAGGATCA 
CCTGGCTTGC CTGGAGCACC AGGCCTGCAG GGCCTCCCAG GATCAAGTGT GATATACTGT 
AGTGTTGGGA ATCCTGGACC ACAAGGAATA AAAGGCAAAG TGGGTCCCCC AGGAGGAAGA 
GGCTCAAAAG GAGAAAAAGG AAATGAAGGA CTCTGTGCCT GTGAGCCTGG ACCCATGGGC 
CCCCCTGGCC CTCCAGGACT TCCTGGGAGG CAGGGGAGTA AGGGAGACTT GGGGCTCCCT 
GGCTGGCTTG GAACAAAAGG TGACCCAGGA CCTCCTGGTG CTGAAGGACC TCCAGGGCTA 
CCAGGAAAGC ATGGTGCCTC TGGACCACCT GGCAACAAAG GGGCGAAGGG TGACATGGTT 
GTATCAAGAG TTAAAGGGCA CAAAGGAGAA AGAGGTCCTG ATGGGCCCCC AGGATTTCCA 
GGGCAGCCAG GATCACATGG TCGGGATGGA CATGCTGGAG AAAAAGGGGA TCCAGGACCC 
CCAGGGGATC ATGAAGATGC GACCCCAGGT GGTAAAGGAT TTCCTGGACC TCTGGGCCCC 
CCGGGCAAAG CAGGACCTTT GGGGCCCCCA GGACTGGGAT TTCCTGGTCC ACCAGGACAG 
CGAGGCCACC CAGGAGTTCC AGGCCACCCA GGTGTGAGGG GCCCTGATGG CTTGAAGGGT 
CAGAAAGGTG ACACAATTTC TTGCAACGTA ACCTACCCTG GGAGGCACGG CCCTCCAGGT 
TTTGATGGAC CTCCAGGTCC GAAGGGATTT CCAGGTCCCC AAGGTGCCCC TGGGCTGAGT 
GGTTCAGATG GGCATAAAGG CAGACCTGGC ACACCAGGAA CAGCGGAAAT ACCAGGTCCA 
CCTGGTTTTC GTGGTGACAT GGGAGATCCG GGTTTTGGAG GTGAAAAGGG GTCCTCCCCT 
GCTGGGCCCC CAGGCCCTCC CGGCTCACCA GGAGTGAATG GTCAGAAAGG AATCCCGGGA 
GACCCTGCAT TTGGTCACCT GGGACCCCCG GGAAAGAGGG GTCTTTCAGG AGTGCCAGGG 
ATAAAAGGAC CCAGAGGTGA TCCGGGATGT CCAGGGGCTG AAGGGCCAGC TGGCATTCCT 
GGATTCCCAG GTCTCAAAGG TCCCAAAGGC AGAGAGGGAC ATGCTGGGTT TCCAGGTGTC 
CCAGGTCCAC CTGGCCATTC CTGTGAAAGA GGTGCTCCAG GGATACCAGG GCAACCGGGA 
CTCCCTGGGT ATCCAGGTAG CCCAGGTGCT CCAGGTGGGA AAGGACAGCT GGGAGATGTG 
GGGCCTCCCG GGCCAGCTGG AATGAAAGGC CTCCCTGGAC TCCCAGGATG GCCTGGGGCA 
CATGGTCCCC CAGGCCTCCC AGGAATCCCA GGTCCCTTTG GAGATGATGG GCTACCTGGT 
CCTCCAGGTC CAAAGGGACC CCAGGGGCTG CCTGGTTTCC CAGGTTTTCC CGGAGAAAGA 
GGAAAGCCTG GTGCAGAGGG ATGTCCTGGC ACAAAGGGAG AACCTGGAGA GAAGGGCATG 
TCTGGCCTTC CCGGAGACCG GGGACTGAGA GGGGCCAAAG GAGCCATAGG ACCTCCCGGA 
GATGAAGGAG AAATGGCTAT CATTTCCCAA AAGGGAACAC CTGGGGAACC TGGACCTCCT 
GGAGATGATG GATTCCCAGG AGAAAGAGGT GATAAAGGAA CTCCCGGGAT GCAAGGGAGA 
AGAGGAGAGC CGGGAAGATA CGGACCACCT GGATTTCAGA GAGGGGAACC TGGTGAGAAA 
GGTCAGCCAG GGCATCCTGG ACCCCCAGGC CCTCCAGGCT CAACTGGTCT AAGAGGGTTC 
ATTGGTTTTC CAGGACTTCC AGGTGACCAG GGTGAGCCAG GTTCTCCAGG TCCCCCTGGA 
TTTTCAGGAA TTGATGGAGC AAGAGGACCT AAAGGAAACA AAGGTGACCC TGCCAGTCAC 
TTTGGTCCAC CTGGTCCAAA GGGTGAGCCA GGTAGCCCTG GATGTCCAGG GCATTTTGGA 
GCATCTGGAG AGCAGGGCTT GCCTGGTATT CAAGGGCCCA GAGGATCACC TGGAAGGCCA 
GGGCCACCTG GCTCCTCTGG ACCACCAGGG TGCCCAGGTG ATCACGGGAT GCCTGGGCTG 
AGGGGACAGC CAGGAGAAAT GGGAGACCCT GGGCCAAGAG GCCTCCAGGG GGATCCAGGG 
ATACCAGGTC CTCCGGGAAT AAAAGGTCCC TCCGGATCAC CTGGCCTGAA CGGCTTGCAT 
GGATTGAAAG GTCAGAAAGG AACTAAAGGT GCTTCAGGTT TGCATGATGT GGGGCCACCT 
GGTCCAGTGG GAATACCTGG GCTAAAAGGG GAGAGAGGAG ACCCTGGGAG CCCAGGAATC 
TCTCCTCCAG GTCCTCGTGG AGAAAAAGGT CCCCCAGGAC CCCCAGGGAG TTCAGGACCA 
CCTGGTCCTG CAGGTGCCAC AGGAAGAGCT CCTAAGGACA TTCCTGACCC GGGTCCACCT 
GGAGATCAGG GACCTCCTGG TCCTGATGGC CCAAGAGGAG CACCTGGGCC TCCAGGCCTC 
CCTGGGAGTG TTGACCTTCT GAGAGGGGAG CCAGGTGACT GTGGTCTACC AGGGCCACCA 
GGTCCCCCTG GCCCACCAGG CCCTCCAGGA TACAAAGGCT TTCCAGGATG TGATGGAAAA 
GATGGCCAGA AAGGACCAAT GGGATTCCCG GGACCGCAGG GACCACATGG ATTTCCTGGG 
CCACCTGGAG AGAAGGGTTT ACCTGGACCT CCAGGGAGAA AAGGGCCCAC TGGTCTTCCA 
GGTCCCAGAG GTGAACCGGG GCCACCTGCA GATGTGGATG ACTGTCCCCG AATCCCAGGC 
CTTCCTGGGG CGCCAGGCAT GAGAGGACCA GAAGGAGCCA TGGGGCTCTC TGGAATGAGA 
GGCCCCCCAG GACCAGCTCA TGGGTGTGTT CTGAGGACCA AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL4A4 ( XM_009444472.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_009442747.2 Pan troglodytes collagen type IV alpha 4 (COL4A4), transcript variant X13, mRNA.

Price & Availability↑

CloneID	OPc80770
Clone ID Related Accession (Same CDS sequence)	XM_016950595.1 , XM_009444472.2 , XM_016950594.1 , XM_016950596.1
Accession Version	XM_009444472.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4245bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	collagen alpha-4(IV) chain isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006470.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 2, 2016 this sequence version replaced XM_009444472.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGTCTC TGCACATAGT ACTAATGAGG TGCTCCTTCA GATTGACCAA GTCCTTGGCC 
ACAGATCCCT GGTCACTTAC ACTCATTCTC TTTTCTGTAC AATATGTATA TGGGAGTGGA 
AAGAAATACG TTGGTCCTTG TGGAGGAAGA GATTGCTCTG TTTGCCACTG TGTTCCTGAA 
AAGGGGTCTC GGGGTCCACC AGGACCACCA GGGCCACAGG GTCCAATTGG ACCCCTGGGA 
GCCCCAGGAC CCATTGGGCT TTCAGGAGAG AAAGGAATGA GAGGGGACCA CGGCCCTCCT 
GGAGCAGCAG GGGACAAAGG AGATAAGGGT TCAACTGGTG TTCCTGGATT TCCAGGTTTA 
GATGGCATAC CTGGGCACCC AGGGCCTCCT GGACCCAGAG GCAAACCTGG TATGAGTGGC 
CACAATGGCT CAAGAGGTGA CCCAGGGTTT CCAGGAGGAA GAGGAGCTCT TGGCCCAGGA 
GGCCCCCTAG GCCATCCTGG GGAAAAGGGA GAAAAAGGAA ATTCAGTGTT CATTTTAGGT 
GCCGTTAAAG GTATTCAGGG AGACAGAGGG GACCCAGGAC TGCCTGGCTT ACCAGGATCT 
TGGGGGGCAG GAGGACCGGC GGGTCCCACA GGATATCCTG GAGAGCCAGG GTTAGTGGGA 
CCTCCGGGCC AACCAGGGCG TCCAGGTTTG AAGGGAAATC CCGGTGTGGG AGTAAAGGGG 
CAAATGGGAG ACCCGGGTGA GGTTGGTCAG CAAGGTTCTC CTGGACCCAC CCTGTTGGTG 
GAGCCACCTG ACTTTCGTCT CTATAAAGGA GAAAAGGGTA TAAAAGGAAT TCCTGGAATG 
GTTGGACTGC CAGGACCACC AGGACGCAAG GGAGAATCTG ATATTGGGGC AAAAGGAGAA 
AAAGGTATTC CTGGATTTCC AGGGCCTCGG GGGGATCCTG GTTCCTATGG ATCTCCAGGT 
TTTCCAGGAT TAAAGGGAGA ACTAGGACTG GTTGGAGATC CTGGGCTATT TGGATTAATT 
GGCCCAAAGG GGGATCCTGG AAATCGAGGG CACCCAGGAC CACCAGGTGT TTTGGTGACT 
CCACCTCTTC CACTCAAAGG CCCACCAGGG GACCCAGGGT TCCCTGGCCG CTATGGAGAA 
ACAGGGGATG TTGGACCACC TGGTCCCCCA GGTCTCTTGG GCAGACCAGG GGAAGCCTGT 
GCAGGCATGA TAGGACCCCC TGGGCCACAA GGATTTCCTG GTCTTCCTGG GCTTCCAGGA 
GAAGCTGGTA TTCCTGGGAG ACCTGATTCT GCTCCAGGAA AACCAGGGAA GCCAGGATCA 
CCTGGCTTGC CTGGAGCACC AGGCCTGCAG GGCCTCCCAG GATCAAGTGT GATATACTGT 
AGTGTTGGGA ATCCTGGACC ACAAGGAATA AAAGGCAAAG TGGGTCCCCC AGGAGGAAGA 
GGCTCAAAAG GAGAAAAAGG AAATGAAGGA CTCTGTGCCT GTGAGCCTGG ACCCATGGGC 
CCCCCTGGCC CTCCAGGACT TCCTGGGAGG CAGGGGAGTA AGGGAGACTT GGGGCTCCCT 
GGCTGGCTTG GAACAAAAGG TGACCCAGGA CCTCCTGGTG CTGAAGGACC TCCAGGGCTA 
CCAGGAAAGC ATGGTGCCTC TGGACCACCT GGCAACAAAG GGGCGAAGGG TGACATGGTT 
GTATCAAGAG TTAAAGGGCA CAAAGGAGAA AGAGGTCCTG ATGGGCCCCC AGGATTTCCA 
GGGCAGCCAG GATCACATGG TCGGGATGGA CATGCTGGAG AAAAAGGGGA TCCAGGACCC 
CCAGGGGATC ATGAAGATGC GACCCCAGGT GGTAAAGGAT TTCCTGGACC TCTGGGCCCC 
CCGGGCAAAG CAGGACCTTT GGGGCCCCCA GGACTGGGAT TTCCTGGTCC ACCAGGACAG 
CGAGGCCACC CAGGAGTTCC AGGCCACCCA GGTGTGAGGG GCCCTGATGG CTTGAAGGGT 
CAGAAAGGTG ACACAATTTC TTGCAACGTA ACCTACCCTG GGAGGCACGG CCCTCCAGGT 
TTTGATGGAC CTCCAGGTCC GAAGGGATTT CCAGGTCCCC AAGGTGCCCC TGGGCTGAGT 
GGTTCAGATG GGCATAAAGG CAGACCTGGC ACACCAGGAA CAGCGGAAAT ACCAGGTCCA 
CCTGGTTTTC GTGGTGACAT GGGAGATCCG GGTTTTGGAG GTGAAAAGGG GTCCTCCCCT 
GCTGGGCCCC CAGGCCCTCC CGGCTCACCA GGAGTGAATG GTCAGAAAGG AATCCCGGGA 
GACCCTGCAT TTGGTCACCT GGGACCCCCG GGAAAGAGGG GTCTTTCAGG AGTGCCAGGG 
ATAAAAGGAC CCAGAGGTGA TCCGGGATGT CCAGGGGCTG AAGGGCCAGC TGGCATTCCT 
GGATTCCCAG GTCTCAAAGG TCCCAAAGGC AGAGAGGGAC ATGCTGGGTT TCCAGGTGTC 
CCAGGTCCAC CTGGCCATTC CTGTGAAAGA GGTGCTCCAG GGATACCAGG GCAACCGGGA 
CTCCCTGGGT ATCCAGGTAG CCCAGGTGCT CCAGGTGGGA AAGGACAGCT GGGAGATGTG 
GGGCCTCCCG GGCCAGCTGG AATGAAAGGC CTCCCTGGAC TCCCAGGATG GCCTGGGGCA 
CATGGTCCCC CAGGCCTCCC AGGAATCCCA GGTCCCTTTG GAGATGATGG GCTACCTGGT 
CCTCCAGGTC CAAAGGGACC CCAGGGGCTG CCTGGTTTCC CAGGTTTTCC CGGAGAAAGA 
GGAAAGCCTG GTGCAGAGGG ATGTCCTGGC ACAAAGGGAG AACCTGGAGA GAAGGGCATG 
TCTGGCCTTC CCGGAGACCG GGGACTGAGA GGGGCCAAAG GAGCCATAGG ACCTCCCGGA 
GATGAAGGAG AAATGGCTAT CATTTCCCAA AAGGGAACAC CTGGGGAACC TGGACCTCCT 
GGAGATGATG GATTCCCAGG AGAAAGAGGT GATAAAGGAA CTCCCGGGAT GCAAGGGAGA 
AGAGGAGAGC CGGGAAGATA CGGACCACCT GGATTTCAGA GAGGGGAACC TGGTGAGAAA 
GGTCAGCCAG GGCATCCTGG ACCCCCAGGC CCTCCAGGCT CAACTGGTCT AAGAGGGTTC 
ATTGGTTTTC CAGGACTTCC AGGTGACCAG GGTGAGCCAG GTTCTCCAGG TCCCCCTGGA 
TTTTCAGGAA TTGATGGAGC AAGAGGACCT AAAGGAAACA AAGGTGACCC TGCCAGTCAC 
TTTGGTCCAC CTGGTCCAAA GGGTGAGCCA GGTAGCCCTG GATGTCCAGG GCATTTTGGA 
GCATCTGGAG AGCAGGGCTT GCCTGGTATT CAAGGGCCCA GAGGATCACC TGGAAGGCCA 
GGGCCACCTG GCTCCTCTGG ACCACCAGGG TGCCCAGGTG ATCACGGGAT GCCTGGGCTG 
AGGGGACAGC CAGGAGAAAT GGGAGACCCT GGGCCAAGAG GCCTCCAGGG GGATCCAGGG 
ATACCAGGTC CTCCGGGAAT AAAAGGTCCC TCCGGATCAC CTGGCCTGAA CGGCTTGCAT 
GGATTGAAAG GTCAGAAAGG AACTAAAGGT GCTTCAGGTT TGCATGATGT GGGGCCACCT 
GGTCCAGTGG GAATACCTGG GCTAAAAGGG GAGAGAGGAG ACCCTGGGAG CCCAGGAATC 
TCTCCTCCAG GTCCTCGTGG AGAAAAAGGT CCCCCAGGAC CCCCAGGGAG TTCAGGACCA 
CCTGGTCCTG CAGGTGCCAC AGGAAGAGCT CCTAAGGACA TTCCTGACCC GGGTCCACCT 
GGAGATCAGG GACCTCCTGG TCCTGATGGC CCAAGAGGAG CACCTGGGCC TCCAGGCCTC 
CCTGGGAGTG TTGACCTTCT GAGAGGGGAG CCAGGTGACT GTGGTCTACC AGGGCCACCA 
GGTCCCCCTG GCCCACCAGG CCCTCCAGGA TACAAAGGCT TTCCAGGATG TGATGGAAAA 
GATGGCCAGA AAGGACCAAT GGGATTCCCG GGACCGCAGG GACCACATGG ATTTCCTGGG 
CCACCTGGAG AGAAGGGTTT ACCTGGACCT CCAGGGAGAA AAGGGCCCAC TGGTCTTCCA 
GGTCCCAGAG GTGAACCGGG GCCACCTGCA GATGTGGATG ACTGTCCCCG AATCCCAGGC 
CTTCCTGGGG CGCCAGGCAT GAGAGGACCA GAAGGAGCCA TGGGGCTCTC TGGAATGAGA 
GGCCCCCCAG GACCAGCTCA TGGGTGTGTT CTGAGGACCA AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009442747.2
CDS	423..4667
Translation	MWSLHIVLMRCSFRLTKSLATDPWSLTLILFSVQYVYGSGKKYVGPCGGRDCSVCHCVPEKGSRGPPGPPGPQGPIGPLGAPGPIGLSGEKGMRGDHGPPGAAGDKGDKGSTGVPGFPGLDGIPGHPGPPGPRGKPGMSGHNGSRGDPGFPGGRGALGPGGPLGHPGEKGEKGNSVFILGAVKGIQGDRGDPGLPGLPGSWGAGGPAGPTGYPGEPGLVGPPGQPGRPGLKGNPGVGVKGQMGDPGEVGQQGSPGPTLLVEPPDFRLYKGEKGIKGIPGMVGLPGPPGRKGESDIGAKGEKGIPGFPGPRGDPGSYGSPGFPGLKGELGLVGDPGLFGLIGPKGDPGNRGHPGPPGVLVTPPLPLKGPPGDPGFPGRYGETGDVGPPGPPGLLGRPGEACAGMIGPPGPQGFPGLPGLPGEAGIPGRPDSAPGKPGKPGSPGLPGAPGLQGLPGSSVIYCSVGNPGPQGIKGKVGPPGGRGSKGEKGNEGLCACEPGPMGPPGPPGLPGRQGSKGDLGLPGWLGTKGDPGPPGAEGPPGLPGKHGASGPPGNKGAKGDMVVSRVKGHKGERGPDGPPGFPGQPGSHGRDGHAGEKGDPGPPGDHEDATPGGKGFPGPLGPPGKAGPLGPPGLGFPGPPGQRGHPGVPGHPGVRGPDGLKGQKGDTISCNVTYPGRHGPPGFDGPPGPKGFPGPQGAPGLSGSDGHKGRPGTPGTAEIPGPPGFRGDMGDPGFGGEKGSSPAGPPGPPGSPGVNGQKGIPGDPAFGHLGPPGKRGLSGVPGIKGPRGDPGCPGAEGPAGIPGFPGLKGPKGREGHAGFPGVPGPPGHSCERGAPGIPGQPGLPGYPGSPGAPGGKGQLGDVGPPGPAGMKGLPGLPGWPGAHGPPGLPGIPGPFGDDGLPGPPGPKGPQGLPGFPGFPGERGKPGAEGCPGTKGEPGEKGMSGLPGDRGLRGAKGAIGPPGDEGEMAIISQKGTPGEPGPPGDDGFPGERGDKGTPGMQGRRGEPGRYGPPGFQRGEPGEKGQPGHPGPPGPPGSTGLRGFIGFPGLPGDQGEPGSPGPPGFSGIDGARGPKGNKGDPASHFGPPGPKGEPGSPGCPGHFGASGEQGLPGIQGPRGSPGRPGPPGSSGPPGCPGDHGMPGLRGQPGEMGDPGPRGLQGDPGIPGPPGIKGPSGSPGLNGLHGLKGQKGTKGASGLHDVGPPGPVGIPGLKGERGDPGSPGISPPGPRGEKGPPGPPGSSGPPGPAGATGRAPKDIPDPGPPGDQGPPGPDGPRGAPGPPGLPGSVDLLRGEPGDCGLPGPPGPPGPPGPPGYKGFPGCDGKDGQKGPMGFPGPQGPHGFPGPPGEKGLPGPPGRKGPTGLPGPRGEPGPPADVDDCPRIPGLPGAPGMRGPEGAMGLSGMRGPPGPAHGCVLRTK

Target ORF information:

RefSeq Version	XM_009444472.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes collagen type IV alpha 4 (COL4A4), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009444472.2

ORF Insert Sequence:

ATGTGGTCTC TGCACATAGT ACTAATGAGG TGCTCCTTCA GATTGACCAA GTCCTTGGCC 
ACAGATCCCT GGTCACTTAC ACTCATTCTC TTTTCTGTAC AATATGTATA TGGGAGTGGA 
AAGAAATACG TTGGTCCTTG TGGAGGAAGA GATTGCTCTG TTTGCCACTG TGTTCCTGAA 
AAGGGGTCTC GGGGTCCACC AGGACCACCA GGGCCACAGG GTCCAATTGG ACCCCTGGGA 
GCCCCAGGAC CCATTGGGCT TTCAGGAGAG AAAGGAATGA GAGGGGACCA CGGCCCTCCT 
GGAGCAGCAG GGGACAAAGG AGATAAGGGT TCAACTGGTG TTCCTGGATT TCCAGGTTTA 
GATGGCATAC CTGGGCACCC AGGGCCTCCT GGACCCAGAG GCAAACCTGG TATGAGTGGC 
CACAATGGCT CAAGAGGTGA CCCAGGGTTT CCAGGAGGAA GAGGAGCTCT TGGCCCAGGA 
GGCCCCCTAG GCCATCCTGG GGAAAAGGGA GAAAAAGGAA ATTCAGTGTT CATTTTAGGT 
GCCGTTAAAG GTATTCAGGG AGACAGAGGG GACCCAGGAC TGCCTGGCTT ACCAGGATCT 
TGGGGGGCAG GAGGACCGGC GGGTCCCACA GGATATCCTG GAGAGCCAGG GTTAGTGGGA 
CCTCCGGGCC AACCAGGGCG TCCAGGTTTG AAGGGAAATC CCGGTGTGGG AGTAAAGGGG 
CAAATGGGAG ACCCGGGTGA GGTTGGTCAG CAAGGTTCTC CTGGACCCAC CCTGTTGGTG 
GAGCCACCTG ACTTTCGTCT CTATAAAGGA GAAAAGGGTA TAAAAGGAAT TCCTGGAATG 
GTTGGACTGC CAGGACCACC AGGACGCAAG GGAGAATCTG ATATTGGGGC AAAAGGAGAA 
AAAGGTATTC CTGGATTTCC AGGGCCTCGG GGGGATCCTG GTTCCTATGG ATCTCCAGGT 
TTTCCAGGAT TAAAGGGAGA ACTAGGACTG GTTGGAGATC CTGGGCTATT TGGATTAATT 
GGCCCAAAGG GGGATCCTGG AAATCGAGGG CACCCAGGAC CACCAGGTGT TTTGGTGACT 
CCACCTCTTC CACTCAAAGG CCCACCAGGG GACCCAGGGT TCCCTGGCCG CTATGGAGAA 
ACAGGGGATG TTGGACCACC TGGTCCCCCA GGTCTCTTGG GCAGACCAGG GGAAGCCTGT 
GCAGGCATGA TAGGACCCCC TGGGCCACAA GGATTTCCTG GTCTTCCTGG GCTTCCAGGA 
GAAGCTGGTA TTCCTGGGAG ACCTGATTCT GCTCCAGGAA AACCAGGGAA GCCAGGATCA 
CCTGGCTTGC CTGGAGCACC AGGCCTGCAG GGCCTCCCAG GATCAAGTGT GATATACTGT 
AGTGTTGGGA ATCCTGGACC ACAAGGAATA AAAGGCAAAG TGGGTCCCCC AGGAGGAAGA 
GGCTCAAAAG GAGAAAAAGG AAATGAAGGA CTCTGTGCCT GTGAGCCTGG ACCCATGGGC 
CCCCCTGGCC CTCCAGGACT TCCTGGGAGG CAGGGGAGTA AGGGAGACTT GGGGCTCCCT 
GGCTGGCTTG GAACAAAAGG TGACCCAGGA CCTCCTGGTG CTGAAGGACC TCCAGGGCTA 
CCAGGAAAGC ATGGTGCCTC TGGACCACCT GGCAACAAAG GGGCGAAGGG TGACATGGTT 
GTATCAAGAG TTAAAGGGCA CAAAGGAGAA AGAGGTCCTG ATGGGCCCCC AGGATTTCCA 
GGGCAGCCAG GATCACATGG TCGGGATGGA CATGCTGGAG AAAAAGGGGA TCCAGGACCC 
CCAGGGGATC ATGAAGATGC GACCCCAGGT GGTAAAGGAT TTCCTGGACC TCTGGGCCCC 
CCGGGCAAAG CAGGACCTTT GGGGCCCCCA GGACTGGGAT TTCCTGGTCC ACCAGGACAG 
CGAGGCCACC CAGGAGTTCC AGGCCACCCA GGTGTGAGGG GCCCTGATGG CTTGAAGGGT 
CAGAAAGGTG ACACAATTTC TTGCAACGTA ACCTACCCTG GGAGGCACGG CCCTCCAGGT 
TTTGATGGAC CTCCAGGTCC GAAGGGATTT CCAGGTCCCC AAGGTGCCCC TGGGCTGAGT 
GGTTCAGATG GGCATAAAGG CAGACCTGGC ACACCAGGAA CAGCGGAAAT ACCAGGTCCA 
CCTGGTTTTC GTGGTGACAT GGGAGATCCG GGTTTTGGAG GTGAAAAGGG GTCCTCCCCT 
GCTGGGCCCC CAGGCCCTCC CGGCTCACCA GGAGTGAATG GTCAGAAAGG AATCCCGGGA 
GACCCTGCAT TTGGTCACCT GGGACCCCCG GGAAAGAGGG GTCTTTCAGG AGTGCCAGGG 
ATAAAAGGAC CCAGAGGTGA TCCGGGATGT CCAGGGGCTG AAGGGCCAGC TGGCATTCCT 
GGATTCCCAG GTCTCAAAGG TCCCAAAGGC AGAGAGGGAC ATGCTGGGTT TCCAGGTGTC 
CCAGGTCCAC CTGGCCATTC CTGTGAAAGA GGTGCTCCAG GGATACCAGG GCAACCGGGA 
CTCCCTGGGT ATCCAGGTAG CCCAGGTGCT CCAGGTGGGA AAGGACAGCT GGGAGATGTG 
GGGCCTCCCG GGCCAGCTGG AATGAAAGGC CTCCCTGGAC TCCCAGGATG GCCTGGGGCA 
CATGGTCCCC CAGGCCTCCC AGGAATCCCA GGTCCCTTTG GAGATGATGG GCTACCTGGT 
CCTCCAGGTC CAAAGGGACC CCAGGGGCTG CCTGGTTTCC CAGGTTTTCC CGGAGAAAGA 
GGAAAGCCTG GTGCAGAGGG ATGTCCTGGC ACAAAGGGAG AACCTGGAGA GAAGGGCATG 
TCTGGCCTTC CCGGAGACCG GGGACTGAGA GGGGCCAAAG GAGCCATAGG ACCTCCCGGA 
GATGAAGGAG AAATGGCTAT CATTTCCCAA AAGGGAACAC CTGGGGAACC TGGACCTCCT 
GGAGATGATG GATTCCCAGG AGAAAGAGGT GATAAAGGAA CTCCCGGGAT GCAAGGGAGA 
AGAGGAGAGC CGGGAAGATA CGGACCACCT GGATTTCAGA GAGGGGAACC TGGTGAGAAA 
GGTCAGCCAG GGCATCCTGG ACCCCCAGGC CCTCCAGGCT CAACTGGTCT AAGAGGGTTC 
ATTGGTTTTC CAGGACTTCC AGGTGACCAG GGTGAGCCAG GTTCTCCAGG TCCCCCTGGA 
TTTTCAGGAA TTGATGGAGC AAGAGGACCT AAAGGAAACA AAGGTGACCC TGCCAGTCAC 
TTTGGTCCAC CTGGTCCAAA GGGTGAGCCA GGTAGCCCTG GATGTCCAGG GCATTTTGGA 
GCATCTGGAG AGCAGGGCTT GCCTGGTATT CAAGGGCCCA GAGGATCACC TGGAAGGCCA 
GGGCCACCTG GCTCCTCTGG ACCACCAGGG TGCCCAGGTG ATCACGGGAT GCCTGGGCTG 
AGGGGACAGC CAGGAGAAAT GGGAGACCCT GGGCCAAGAG GCCTCCAGGG GGATCCAGGG 
ATACCAGGTC CTCCGGGAAT AAAAGGTCCC TCCGGATCAC CTGGCCTGAA CGGCTTGCAT 
GGATTGAAAG GTCAGAAAGG AACTAAAGGT GCTTCAGGTT TGCATGATGT GGGGCCACCT 
GGTCCAGTGG GAATACCTGG GCTAAAAGGG GAGAGAGGAG ACCCTGGGAG CCCAGGAATC 
TCTCCTCCAG GTCCTCGTGG AGAAAAAGGT CCCCCAGGAC CCCCAGGGAG TTCAGGACCA 
CCTGGTCCTG CAGGTGCCAC AGGAAGAGCT CCTAAGGACA TTCCTGACCC GGGTCCACCT 
GGAGATCAGG GACCTCCTGG TCCTGATGGC CCAAGAGGAG CACCTGGGCC TCCAGGCCTC 
CCTGGGAGTG TTGACCTTCT GAGAGGGGAG CCAGGTGACT GTGGTCTACC AGGGCCACCA 
GGTCCCCCTG GCCCACCAGG CCCTCCAGGA TACAAAGGCT TTCCAGGATG TGATGGAAAA 
GATGGCCAGA AAGGACCAAT GGGATTCCCG GGACCGCAGG GACCACATGG ATTTCCTGGG 
CCACCTGGAG AGAAGGGTTT ACCTGGACCT CCAGGGAGAA AAGGGCCCAC TGGTCTTCCA 
GGTCCCAGAG GTGAACCGGG GCCACCTGCA GATGTGGATG ACTGTCCCCG AATCCCAGGC 
CTTCCTGGGG CGCCAGGCAT GAGAGGACCA GAAGGAGCCA TGGGGCTCTC TGGAATGAGA 
GGCCCCCCAG GACCAGCTCA TGGGTGTGTT CTGAGGACCA AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL4A4 ( XM_016950594.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016806083.1 Pan troglodytes collagen type IV alpha 4 (COL4A4), transcript variant X14, mRNA.

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CloneID	OPc80770
Clone ID Related Accession (Same CDS sequence)	XM_016950595.1 , XM_009444472.2 , XM_016950594.1 , XM_016950596.1
Accession Version	XM_016950594.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4245bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	collagen alpha-4(IV) chain isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006470.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGTCTC TGCACATAGT ACTAATGAGG TGCTCCTTCA GATTGACCAA GTCCTTGGCC 
ACAGATCCCT GGTCACTTAC ACTCATTCTC TTTTCTGTAC AATATGTATA TGGGAGTGGA 
AAGAAATACG TTGGTCCTTG TGGAGGAAGA GATTGCTCTG TTTGCCACTG TGTTCCTGAA 
AAGGGGTCTC GGGGTCCACC AGGACCACCA GGGCCACAGG GTCCAATTGG ACCCCTGGGA 
GCCCCAGGAC CCATTGGGCT TTCAGGAGAG AAAGGAATGA GAGGGGACCA CGGCCCTCCT 
GGAGCAGCAG GGGACAAAGG AGATAAGGGT TCAACTGGTG TTCCTGGATT TCCAGGTTTA 
GATGGCATAC CTGGGCACCC AGGGCCTCCT GGACCCAGAG GCAAACCTGG TATGAGTGGC 
CACAATGGCT CAAGAGGTGA CCCAGGGTTT CCAGGAGGAA GAGGAGCTCT TGGCCCAGGA 
GGCCCCCTAG GCCATCCTGG GGAAAAGGGA GAAAAAGGAA ATTCAGTGTT CATTTTAGGT 
GCCGTTAAAG GTATTCAGGG AGACAGAGGG GACCCAGGAC TGCCTGGCTT ACCAGGATCT 
TGGGGGGCAG GAGGACCGGC GGGTCCCACA GGATATCCTG GAGAGCCAGG GTTAGTGGGA 
CCTCCGGGCC AACCAGGGCG TCCAGGTTTG AAGGGAAATC CCGGTGTGGG AGTAAAGGGG 
CAAATGGGAG ACCCGGGTGA GGTTGGTCAG CAAGGTTCTC CTGGACCCAC CCTGTTGGTG 
GAGCCACCTG ACTTTCGTCT CTATAAAGGA GAAAAGGGTA TAAAAGGAAT TCCTGGAATG 
GTTGGACTGC CAGGACCACC AGGACGCAAG GGAGAATCTG ATATTGGGGC AAAAGGAGAA 
AAAGGTATTC CTGGATTTCC AGGGCCTCGG GGGGATCCTG GTTCCTATGG ATCTCCAGGT 
TTTCCAGGAT TAAAGGGAGA ACTAGGACTG GTTGGAGATC CTGGGCTATT TGGATTAATT 
GGCCCAAAGG GGGATCCTGG AAATCGAGGG CACCCAGGAC CACCAGGTGT TTTGGTGACT 
CCACCTCTTC CACTCAAAGG CCCACCAGGG GACCCAGGGT TCCCTGGCCG CTATGGAGAA 
ACAGGGGATG TTGGACCACC TGGTCCCCCA GGTCTCTTGG GCAGACCAGG GGAAGCCTGT 
GCAGGCATGA TAGGACCCCC TGGGCCACAA GGATTTCCTG GTCTTCCTGG GCTTCCAGGA 
GAAGCTGGTA TTCCTGGGAG ACCTGATTCT GCTCCAGGAA AACCAGGGAA GCCAGGATCA 
CCTGGCTTGC CTGGAGCACC AGGCCTGCAG GGCCTCCCAG GATCAAGTGT GATATACTGT 
AGTGTTGGGA ATCCTGGACC ACAAGGAATA AAAGGCAAAG TGGGTCCCCC AGGAGGAAGA 
GGCTCAAAAG GAGAAAAAGG AAATGAAGGA CTCTGTGCCT GTGAGCCTGG ACCCATGGGC 
CCCCCTGGCC CTCCAGGACT TCCTGGGAGG CAGGGGAGTA AGGGAGACTT GGGGCTCCCT 
GGCTGGCTTG GAACAAAAGG TGACCCAGGA CCTCCTGGTG CTGAAGGACC TCCAGGGCTA 
CCAGGAAAGC ATGGTGCCTC TGGACCACCT GGCAACAAAG GGGCGAAGGG TGACATGGTT 
GTATCAAGAG TTAAAGGGCA CAAAGGAGAA AGAGGTCCTG ATGGGCCCCC AGGATTTCCA 
GGGCAGCCAG GATCACATGG TCGGGATGGA CATGCTGGAG AAAAAGGGGA TCCAGGACCC 
CCAGGGGATC ATGAAGATGC GACCCCAGGT GGTAAAGGAT TTCCTGGACC TCTGGGCCCC 
CCGGGCAAAG CAGGACCTTT GGGGCCCCCA GGACTGGGAT TTCCTGGTCC ACCAGGACAG 
CGAGGCCACC CAGGAGTTCC AGGCCACCCA GGTGTGAGGG GCCCTGATGG CTTGAAGGGT 
CAGAAAGGTG ACACAATTTC TTGCAACGTA ACCTACCCTG GGAGGCACGG CCCTCCAGGT 
TTTGATGGAC CTCCAGGTCC GAAGGGATTT CCAGGTCCCC AAGGTGCCCC TGGGCTGAGT 
GGTTCAGATG GGCATAAAGG CAGACCTGGC ACACCAGGAA CAGCGGAAAT ACCAGGTCCA 
CCTGGTTTTC GTGGTGACAT GGGAGATCCG GGTTTTGGAG GTGAAAAGGG GTCCTCCCCT 
GCTGGGCCCC CAGGCCCTCC CGGCTCACCA GGAGTGAATG GTCAGAAAGG AATCCCGGGA 
GACCCTGCAT TTGGTCACCT GGGACCCCCG GGAAAGAGGG GTCTTTCAGG AGTGCCAGGG 
ATAAAAGGAC CCAGAGGTGA TCCGGGATGT CCAGGGGCTG AAGGGCCAGC TGGCATTCCT 
GGATTCCCAG GTCTCAAAGG TCCCAAAGGC AGAGAGGGAC ATGCTGGGTT TCCAGGTGTC 
CCAGGTCCAC CTGGCCATTC CTGTGAAAGA GGTGCTCCAG GGATACCAGG GCAACCGGGA 
CTCCCTGGGT ATCCAGGTAG CCCAGGTGCT CCAGGTGGGA AAGGACAGCT GGGAGATGTG 
GGGCCTCCCG GGCCAGCTGG AATGAAAGGC CTCCCTGGAC TCCCAGGATG GCCTGGGGCA 
CATGGTCCCC CAGGCCTCCC AGGAATCCCA GGTCCCTTTG GAGATGATGG GCTACCTGGT 
CCTCCAGGTC CAAAGGGACC CCAGGGGCTG CCTGGTTTCC CAGGTTTTCC CGGAGAAAGA 
GGAAAGCCTG GTGCAGAGGG ATGTCCTGGC ACAAAGGGAG AACCTGGAGA GAAGGGCATG 
TCTGGCCTTC CCGGAGACCG GGGACTGAGA GGGGCCAAAG GAGCCATAGG ACCTCCCGGA 
GATGAAGGAG AAATGGCTAT CATTTCCCAA AAGGGAACAC CTGGGGAACC TGGACCTCCT 
GGAGATGATG GATTCCCAGG AGAAAGAGGT GATAAAGGAA CTCCCGGGAT GCAAGGGAGA 
AGAGGAGAGC CGGGAAGATA CGGACCACCT GGATTTCAGA GAGGGGAACC TGGTGAGAAA 
GGTCAGCCAG GGCATCCTGG ACCCCCAGGC CCTCCAGGCT CAACTGGTCT AAGAGGGTTC 
ATTGGTTTTC CAGGACTTCC AGGTGACCAG GGTGAGCCAG GTTCTCCAGG TCCCCCTGGA 
TTTTCAGGAA TTGATGGAGC AAGAGGACCT AAAGGAAACA AAGGTGACCC TGCCAGTCAC 
TTTGGTCCAC CTGGTCCAAA GGGTGAGCCA GGTAGCCCTG GATGTCCAGG GCATTTTGGA 
GCATCTGGAG AGCAGGGCTT GCCTGGTATT CAAGGGCCCA GAGGATCACC TGGAAGGCCA 
GGGCCACCTG GCTCCTCTGG ACCACCAGGG TGCCCAGGTG ATCACGGGAT GCCTGGGCTG 
AGGGGACAGC CAGGAGAAAT GGGAGACCCT GGGCCAAGAG GCCTCCAGGG GGATCCAGGG 
ATACCAGGTC CTCCGGGAAT AAAAGGTCCC TCCGGATCAC CTGGCCTGAA CGGCTTGCAT 
GGATTGAAAG GTCAGAAAGG AACTAAAGGT GCTTCAGGTT TGCATGATGT GGGGCCACCT 
GGTCCAGTGG GAATACCTGG GCTAAAAGGG GAGAGAGGAG ACCCTGGGAG CCCAGGAATC 
TCTCCTCCAG GTCCTCGTGG AGAAAAAGGT CCCCCAGGAC CCCCAGGGAG TTCAGGACCA 
CCTGGTCCTG CAGGTGCCAC AGGAAGAGCT CCTAAGGACA TTCCTGACCC GGGTCCACCT 
GGAGATCAGG GACCTCCTGG TCCTGATGGC CCAAGAGGAG CACCTGGGCC TCCAGGCCTC 
CCTGGGAGTG TTGACCTTCT GAGAGGGGAG CCAGGTGACT GTGGTCTACC AGGGCCACCA 
GGTCCCCCTG GCCCACCAGG CCCTCCAGGA TACAAAGGCT TTCCAGGATG TGATGGAAAA 
GATGGCCAGA AAGGACCAAT GGGATTCCCG GGACCGCAGG GACCACATGG ATTTCCTGGG 
CCACCTGGAG AGAAGGGTTT ACCTGGACCT CCAGGGAGAA AAGGGCCCAC TGGTCTTCCA 
GGTCCCAGAG GTGAACCGGG GCCACCTGCA GATGTGGATG ACTGTCCCCG AATCCCAGGC 
CTTCCTGGGG CGCCAGGCAT GAGAGGACCA GAAGGAGCCA TGGGGCTCTC TGGAATGAGA 
GGCCCCCCAG GACCAGCTCA TGGGTGTGTT CTGAGGACCA AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016806083.1
CDS	423..4667
Translation	MWSLHIVLMRCSFRLTKSLATDPWSLTLILFSVQYVYGSGKKYVGPCGGRDCSVCHCVPEKGSRGPPGPPGPQGPIGPLGAPGPIGLSGEKGMRGDHGPPGAAGDKGDKGSTGVPGFPGLDGIPGHPGPPGPRGKPGMSGHNGSRGDPGFPGGRGALGPGGPLGHPGEKGEKGNSVFILGAVKGIQGDRGDPGLPGLPGSWGAGGPAGPTGYPGEPGLVGPPGQPGRPGLKGNPGVGVKGQMGDPGEVGQQGSPGPTLLVEPPDFRLYKGEKGIKGIPGMVGLPGPPGRKGESDIGAKGEKGIPGFPGPRGDPGSYGSPGFPGLKGELGLVGDPGLFGLIGPKGDPGNRGHPGPPGVLVTPPLPLKGPPGDPGFPGRYGETGDVGPPGPPGLLGRPGEACAGMIGPPGPQGFPGLPGLPGEAGIPGRPDSAPGKPGKPGSPGLPGAPGLQGLPGSSVIYCSVGNPGPQGIKGKVGPPGGRGSKGEKGNEGLCACEPGPMGPPGPPGLPGRQGSKGDLGLPGWLGTKGDPGPPGAEGPPGLPGKHGASGPPGNKGAKGDMVVSRVKGHKGERGPDGPPGFPGQPGSHGRDGHAGEKGDPGPPGDHEDATPGGKGFPGPLGPPGKAGPLGPPGLGFPGPPGQRGHPGVPGHPGVRGPDGLKGQKGDTISCNVTYPGRHGPPGFDGPPGPKGFPGPQGAPGLSGSDGHKGRPGTPGTAEIPGPPGFRGDMGDPGFGGEKGSSPAGPPGPPGSPGVNGQKGIPGDPAFGHLGPPGKRGLSGVPGIKGPRGDPGCPGAEGPAGIPGFPGLKGPKGREGHAGFPGVPGPPGHSCERGAPGIPGQPGLPGYPGSPGAPGGKGQLGDVGPPGPAGMKGLPGLPGWPGAHGPPGLPGIPGPFGDDGLPGPPGPKGPQGLPGFPGFPGERGKPGAEGCPGTKGEPGEKGMSGLPGDRGLRGAKGAIGPPGDEGEMAIISQKGTPGEPGPPGDDGFPGERGDKGTPGMQGRRGEPGRYGPPGFQRGEPGEKGQPGHPGPPGPPGSTGLRGFIGFPGLPGDQGEPGSPGPPGFSGIDGARGPKGNKGDPASHFGPPGPKGEPGSPGCPGHFGASGEQGLPGIQGPRGSPGRPGPPGSSGPPGCPGDHGMPGLRGQPGEMGDPGPRGLQGDPGIPGPPGIKGPSGSPGLNGLHGLKGQKGTKGASGLHDVGPPGPVGIPGLKGERGDPGSPGISPPGPRGEKGPPGPPGSSGPPGPAGATGRAPKDIPDPGPPGDQGPPGPDGPRGAPGPPGLPGSVDLLRGEPGDCGLPGPPGPPGPPGPPGYKGFPGCDGKDGQKGPMGFPGPQGPHGFPGPPGEKGLPGPPGRKGPTGLPGPRGEPGPPADVDDCPRIPGLPGAPGMRGPEGAMGLSGMRGPPGPAHGCVLRTK

Target ORF information:

RefSeq Version	XM_016950594.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes collagen type IV alpha 4 (COL4A4), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016950594.1

ORF Insert Sequence:

ATGTGGTCTC TGCACATAGT ACTAATGAGG TGCTCCTTCA GATTGACCAA GTCCTTGGCC 
ACAGATCCCT GGTCACTTAC ACTCATTCTC TTTTCTGTAC AATATGTATA TGGGAGTGGA 
AAGAAATACG TTGGTCCTTG TGGAGGAAGA GATTGCTCTG TTTGCCACTG TGTTCCTGAA 
AAGGGGTCTC GGGGTCCACC AGGACCACCA GGGCCACAGG GTCCAATTGG ACCCCTGGGA 
GCCCCAGGAC CCATTGGGCT TTCAGGAGAG AAAGGAATGA GAGGGGACCA CGGCCCTCCT 
GGAGCAGCAG GGGACAAAGG AGATAAGGGT TCAACTGGTG TTCCTGGATT TCCAGGTTTA 
GATGGCATAC CTGGGCACCC AGGGCCTCCT GGACCCAGAG GCAAACCTGG TATGAGTGGC 
CACAATGGCT CAAGAGGTGA CCCAGGGTTT CCAGGAGGAA GAGGAGCTCT TGGCCCAGGA 
GGCCCCCTAG GCCATCCTGG GGAAAAGGGA GAAAAAGGAA ATTCAGTGTT CATTTTAGGT 
GCCGTTAAAG GTATTCAGGG AGACAGAGGG GACCCAGGAC TGCCTGGCTT ACCAGGATCT 
TGGGGGGCAG GAGGACCGGC GGGTCCCACA GGATATCCTG GAGAGCCAGG GTTAGTGGGA 
CCTCCGGGCC AACCAGGGCG TCCAGGTTTG AAGGGAAATC CCGGTGTGGG AGTAAAGGGG 
CAAATGGGAG ACCCGGGTGA GGTTGGTCAG CAAGGTTCTC CTGGACCCAC CCTGTTGGTG 
GAGCCACCTG ACTTTCGTCT CTATAAAGGA GAAAAGGGTA TAAAAGGAAT TCCTGGAATG 
GTTGGACTGC CAGGACCACC AGGACGCAAG GGAGAATCTG ATATTGGGGC AAAAGGAGAA 
AAAGGTATTC CTGGATTTCC AGGGCCTCGG GGGGATCCTG GTTCCTATGG ATCTCCAGGT 
TTTCCAGGAT TAAAGGGAGA ACTAGGACTG GTTGGAGATC CTGGGCTATT TGGATTAATT 
GGCCCAAAGG GGGATCCTGG AAATCGAGGG CACCCAGGAC CACCAGGTGT TTTGGTGACT 
CCACCTCTTC CACTCAAAGG CCCACCAGGG GACCCAGGGT TCCCTGGCCG CTATGGAGAA 
ACAGGGGATG TTGGACCACC TGGTCCCCCA GGTCTCTTGG GCAGACCAGG GGAAGCCTGT 
GCAGGCATGA TAGGACCCCC TGGGCCACAA GGATTTCCTG GTCTTCCTGG GCTTCCAGGA 
GAAGCTGGTA TTCCTGGGAG ACCTGATTCT GCTCCAGGAA AACCAGGGAA GCCAGGATCA 
CCTGGCTTGC CTGGAGCACC AGGCCTGCAG GGCCTCCCAG GATCAAGTGT GATATACTGT 
AGTGTTGGGA ATCCTGGACC ACAAGGAATA AAAGGCAAAG TGGGTCCCCC AGGAGGAAGA 
GGCTCAAAAG GAGAAAAAGG AAATGAAGGA CTCTGTGCCT GTGAGCCTGG ACCCATGGGC 
CCCCCTGGCC CTCCAGGACT TCCTGGGAGG CAGGGGAGTA AGGGAGACTT GGGGCTCCCT 
GGCTGGCTTG GAACAAAAGG TGACCCAGGA CCTCCTGGTG CTGAAGGACC TCCAGGGCTA 
CCAGGAAAGC ATGGTGCCTC TGGACCACCT GGCAACAAAG GGGCGAAGGG TGACATGGTT 
GTATCAAGAG TTAAAGGGCA CAAAGGAGAA AGAGGTCCTG ATGGGCCCCC AGGATTTCCA 
GGGCAGCCAG GATCACATGG TCGGGATGGA CATGCTGGAG AAAAAGGGGA TCCAGGACCC 
CCAGGGGATC ATGAAGATGC GACCCCAGGT GGTAAAGGAT TTCCTGGACC TCTGGGCCCC 
CCGGGCAAAG CAGGACCTTT GGGGCCCCCA GGACTGGGAT TTCCTGGTCC ACCAGGACAG 
CGAGGCCACC CAGGAGTTCC AGGCCACCCA GGTGTGAGGG GCCCTGATGG CTTGAAGGGT 
CAGAAAGGTG ACACAATTTC TTGCAACGTA ACCTACCCTG GGAGGCACGG CCCTCCAGGT 
TTTGATGGAC CTCCAGGTCC GAAGGGATTT CCAGGTCCCC AAGGTGCCCC TGGGCTGAGT 
GGTTCAGATG GGCATAAAGG CAGACCTGGC ACACCAGGAA CAGCGGAAAT ACCAGGTCCA 
CCTGGTTTTC GTGGTGACAT GGGAGATCCG GGTTTTGGAG GTGAAAAGGG GTCCTCCCCT 
GCTGGGCCCC CAGGCCCTCC CGGCTCACCA GGAGTGAATG GTCAGAAAGG AATCCCGGGA 
GACCCTGCAT TTGGTCACCT GGGACCCCCG GGAAAGAGGG GTCTTTCAGG AGTGCCAGGG 
ATAAAAGGAC CCAGAGGTGA TCCGGGATGT CCAGGGGCTG AAGGGCCAGC TGGCATTCCT 
GGATTCCCAG GTCTCAAAGG TCCCAAAGGC AGAGAGGGAC ATGCTGGGTT TCCAGGTGTC 
CCAGGTCCAC CTGGCCATTC CTGTGAAAGA GGTGCTCCAG GGATACCAGG GCAACCGGGA 
CTCCCTGGGT ATCCAGGTAG CCCAGGTGCT CCAGGTGGGA AAGGACAGCT GGGAGATGTG 
GGGCCTCCCG GGCCAGCTGG AATGAAAGGC CTCCCTGGAC TCCCAGGATG GCCTGGGGCA 
CATGGTCCCC CAGGCCTCCC AGGAATCCCA GGTCCCTTTG GAGATGATGG GCTACCTGGT 
CCTCCAGGTC CAAAGGGACC CCAGGGGCTG CCTGGTTTCC CAGGTTTTCC CGGAGAAAGA 
GGAAAGCCTG GTGCAGAGGG ATGTCCTGGC ACAAAGGGAG AACCTGGAGA GAAGGGCATG 
TCTGGCCTTC CCGGAGACCG GGGACTGAGA GGGGCCAAAG GAGCCATAGG ACCTCCCGGA 
GATGAAGGAG AAATGGCTAT CATTTCCCAA AAGGGAACAC CTGGGGAACC TGGACCTCCT 
GGAGATGATG GATTCCCAGG AGAAAGAGGT GATAAAGGAA CTCCCGGGAT GCAAGGGAGA 
AGAGGAGAGC CGGGAAGATA CGGACCACCT GGATTTCAGA GAGGGGAACC TGGTGAGAAA 
GGTCAGCCAG GGCATCCTGG ACCCCCAGGC CCTCCAGGCT CAACTGGTCT AAGAGGGTTC 
ATTGGTTTTC CAGGACTTCC AGGTGACCAG GGTGAGCCAG GTTCTCCAGG TCCCCCTGGA 
TTTTCAGGAA TTGATGGAGC AAGAGGACCT AAAGGAAACA AAGGTGACCC TGCCAGTCAC 
TTTGGTCCAC CTGGTCCAAA GGGTGAGCCA GGTAGCCCTG GATGTCCAGG GCATTTTGGA 
GCATCTGGAG AGCAGGGCTT GCCTGGTATT CAAGGGCCCA GAGGATCACC TGGAAGGCCA 
GGGCCACCTG GCTCCTCTGG ACCACCAGGG TGCCCAGGTG ATCACGGGAT GCCTGGGCTG 
AGGGGACAGC CAGGAGAAAT GGGAGACCCT GGGCCAAGAG GCCTCCAGGG GGATCCAGGG 
ATACCAGGTC CTCCGGGAAT AAAAGGTCCC TCCGGATCAC CTGGCCTGAA CGGCTTGCAT 
GGATTGAAAG GTCAGAAAGG AACTAAAGGT GCTTCAGGTT TGCATGATGT GGGGCCACCT 
GGTCCAGTGG GAATACCTGG GCTAAAAGGG GAGAGAGGAG ACCCTGGGAG CCCAGGAATC 
TCTCCTCCAG GTCCTCGTGG AGAAAAAGGT CCCCCAGGAC CCCCAGGGAG TTCAGGACCA 
CCTGGTCCTG CAGGTGCCAC AGGAAGAGCT CCTAAGGACA TTCCTGACCC GGGTCCACCT 
GGAGATCAGG GACCTCCTGG TCCTGATGGC CCAAGAGGAG CACCTGGGCC TCCAGGCCTC 
CCTGGGAGTG TTGACCTTCT GAGAGGGGAG CCAGGTGACT GTGGTCTACC AGGGCCACCA 
GGTCCCCCTG GCCCACCAGG CCCTCCAGGA TACAAAGGCT TTCCAGGATG TGATGGAAAA 
GATGGCCAGA AAGGACCAAT GGGATTCCCG GGACCGCAGG GACCACATGG ATTTCCTGGG 
CCACCTGGAG AGAAGGGTTT ACCTGGACCT CCAGGGAGAA AAGGGCCCAC TGGTCTTCCA 
GGTCCCAGAG GTGAACCGGG GCCACCTGCA GATGTGGATG ACTGTCCCCG AATCCCAGGC 
CTTCCTGGGG CGCCAGGCAT GAGAGGACCA GAAGGAGCCA TGGGGCTCTC TGGAATGAGA 
GGCCCCCCAG GACCAGCTCA TGGGTGTGTT CTGAGGACCA AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL4A4 ( XM_016950596.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016806085.1 Pan troglodytes collagen type IV alpha 4 (COL4A4), transcript variant X16, mRNA.

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CloneID	OPc80770
Clone ID Related Accession (Same CDS sequence)	XM_016950595.1 , XM_009444472.2 , XM_016950594.1 , XM_016950596.1
Accession Version	XM_016950596.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4245bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	collagen alpha-4(IV) chain isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006470.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGTCTC TGCACATAGT ACTAATGAGG TGCTCCTTCA GATTGACCAA GTCCTTGGCC 
ACAGATCCCT GGTCACTTAC ACTCATTCTC TTTTCTGTAC AATATGTATA TGGGAGTGGA 
AAGAAATACG TTGGTCCTTG TGGAGGAAGA GATTGCTCTG TTTGCCACTG TGTTCCTGAA 
AAGGGGTCTC GGGGTCCACC AGGACCACCA GGGCCACAGG GTCCAATTGG ACCCCTGGGA 
GCCCCAGGAC CCATTGGGCT TTCAGGAGAG AAAGGAATGA GAGGGGACCA CGGCCCTCCT 
GGAGCAGCAG GGGACAAAGG AGATAAGGGT TCAACTGGTG TTCCTGGATT TCCAGGTTTA 
GATGGCATAC CTGGGCACCC AGGGCCTCCT GGACCCAGAG GCAAACCTGG TATGAGTGGC 
CACAATGGCT CAAGAGGTGA CCCAGGGTTT CCAGGAGGAA GAGGAGCTCT TGGCCCAGGA 
GGCCCCCTAG GCCATCCTGG GGAAAAGGGA GAAAAAGGAA ATTCAGTGTT CATTTTAGGT 
GCCGTTAAAG GTATTCAGGG AGACAGAGGG GACCCAGGAC TGCCTGGCTT ACCAGGATCT 
TGGGGGGCAG GAGGACCGGC GGGTCCCACA GGATATCCTG GAGAGCCAGG GTTAGTGGGA 
CCTCCGGGCC AACCAGGGCG TCCAGGTTTG AAGGGAAATC CCGGTGTGGG AGTAAAGGGG 
CAAATGGGAG ACCCGGGTGA GGTTGGTCAG CAAGGTTCTC CTGGACCCAC CCTGTTGGTG 
GAGCCACCTG ACTTTCGTCT CTATAAAGGA GAAAAGGGTA TAAAAGGAAT TCCTGGAATG 
GTTGGACTGC CAGGACCACC AGGACGCAAG GGAGAATCTG ATATTGGGGC AAAAGGAGAA 
AAAGGTATTC CTGGATTTCC AGGGCCTCGG GGGGATCCTG GTTCCTATGG ATCTCCAGGT 
TTTCCAGGAT TAAAGGGAGA ACTAGGACTG GTTGGAGATC CTGGGCTATT TGGATTAATT 
GGCCCAAAGG GGGATCCTGG AAATCGAGGG CACCCAGGAC CACCAGGTGT TTTGGTGACT 
CCACCTCTTC CACTCAAAGG CCCACCAGGG GACCCAGGGT TCCCTGGCCG CTATGGAGAA 
ACAGGGGATG TTGGACCACC TGGTCCCCCA GGTCTCTTGG GCAGACCAGG GGAAGCCTGT 
GCAGGCATGA TAGGACCCCC TGGGCCACAA GGATTTCCTG GTCTTCCTGG GCTTCCAGGA 
GAAGCTGGTA TTCCTGGGAG ACCTGATTCT GCTCCAGGAA AACCAGGGAA GCCAGGATCA 
CCTGGCTTGC CTGGAGCACC AGGCCTGCAG GGCCTCCCAG GATCAAGTGT GATATACTGT 
AGTGTTGGGA ATCCTGGACC ACAAGGAATA AAAGGCAAAG TGGGTCCCCC AGGAGGAAGA 
GGCTCAAAAG GAGAAAAAGG AAATGAAGGA CTCTGTGCCT GTGAGCCTGG ACCCATGGGC 
CCCCCTGGCC CTCCAGGACT TCCTGGGAGG CAGGGGAGTA AGGGAGACTT GGGGCTCCCT 
GGCTGGCTTG GAACAAAAGG TGACCCAGGA CCTCCTGGTG CTGAAGGACC TCCAGGGCTA 
CCAGGAAAGC ATGGTGCCTC TGGACCACCT GGCAACAAAG GGGCGAAGGG TGACATGGTT 
GTATCAAGAG TTAAAGGGCA CAAAGGAGAA AGAGGTCCTG ATGGGCCCCC AGGATTTCCA 
GGGCAGCCAG GATCACATGG TCGGGATGGA CATGCTGGAG AAAAAGGGGA TCCAGGACCC 
CCAGGGGATC ATGAAGATGC GACCCCAGGT GGTAAAGGAT TTCCTGGACC TCTGGGCCCC 
CCGGGCAAAG CAGGACCTTT GGGGCCCCCA GGACTGGGAT TTCCTGGTCC ACCAGGACAG 
CGAGGCCACC CAGGAGTTCC AGGCCACCCA GGTGTGAGGG GCCCTGATGG CTTGAAGGGT 
CAGAAAGGTG ACACAATTTC TTGCAACGTA ACCTACCCTG GGAGGCACGG CCCTCCAGGT 
TTTGATGGAC CTCCAGGTCC GAAGGGATTT CCAGGTCCCC AAGGTGCCCC TGGGCTGAGT 
GGTTCAGATG GGCATAAAGG CAGACCTGGC ACACCAGGAA CAGCGGAAAT ACCAGGTCCA 
CCTGGTTTTC GTGGTGACAT GGGAGATCCG GGTTTTGGAG GTGAAAAGGG GTCCTCCCCT 
GCTGGGCCCC CAGGCCCTCC CGGCTCACCA GGAGTGAATG GTCAGAAAGG AATCCCGGGA 
GACCCTGCAT TTGGTCACCT GGGACCCCCG GGAAAGAGGG GTCTTTCAGG AGTGCCAGGG 
ATAAAAGGAC CCAGAGGTGA TCCGGGATGT CCAGGGGCTG AAGGGCCAGC TGGCATTCCT 
GGATTCCCAG GTCTCAAAGG TCCCAAAGGC AGAGAGGGAC ATGCTGGGTT TCCAGGTGTC 
CCAGGTCCAC CTGGCCATTC CTGTGAAAGA GGTGCTCCAG GGATACCAGG GCAACCGGGA 
CTCCCTGGGT ATCCAGGTAG CCCAGGTGCT CCAGGTGGGA AAGGACAGCT GGGAGATGTG 
GGGCCTCCCG GGCCAGCTGG AATGAAAGGC CTCCCTGGAC TCCCAGGATG GCCTGGGGCA 
CATGGTCCCC CAGGCCTCCC AGGAATCCCA GGTCCCTTTG GAGATGATGG GCTACCTGGT 
CCTCCAGGTC CAAAGGGACC CCAGGGGCTG CCTGGTTTCC CAGGTTTTCC CGGAGAAAGA 
GGAAAGCCTG GTGCAGAGGG ATGTCCTGGC ACAAAGGGAG AACCTGGAGA GAAGGGCATG 
TCTGGCCTTC CCGGAGACCG GGGACTGAGA GGGGCCAAAG GAGCCATAGG ACCTCCCGGA 
GATGAAGGAG AAATGGCTAT CATTTCCCAA AAGGGAACAC CTGGGGAACC TGGACCTCCT 
GGAGATGATG GATTCCCAGG AGAAAGAGGT GATAAAGGAA CTCCCGGGAT GCAAGGGAGA 
AGAGGAGAGC CGGGAAGATA CGGACCACCT GGATTTCAGA GAGGGGAACC TGGTGAGAAA 
GGTCAGCCAG GGCATCCTGG ACCCCCAGGC CCTCCAGGCT CAACTGGTCT AAGAGGGTTC 
ATTGGTTTTC CAGGACTTCC AGGTGACCAG GGTGAGCCAG GTTCTCCAGG TCCCCCTGGA 
TTTTCAGGAA TTGATGGAGC AAGAGGACCT AAAGGAAACA AAGGTGACCC TGCCAGTCAC 
TTTGGTCCAC CTGGTCCAAA GGGTGAGCCA GGTAGCCCTG GATGTCCAGG GCATTTTGGA 
GCATCTGGAG AGCAGGGCTT GCCTGGTATT CAAGGGCCCA GAGGATCACC TGGAAGGCCA 
GGGCCACCTG GCTCCTCTGG ACCACCAGGG TGCCCAGGTG ATCACGGGAT GCCTGGGCTG 
AGGGGACAGC CAGGAGAAAT GGGAGACCCT GGGCCAAGAG GCCTCCAGGG GGATCCAGGG 
ATACCAGGTC CTCCGGGAAT AAAAGGTCCC TCCGGATCAC CTGGCCTGAA CGGCTTGCAT 
GGATTGAAAG GTCAGAAAGG AACTAAAGGT GCTTCAGGTT TGCATGATGT GGGGCCACCT 
GGTCCAGTGG GAATACCTGG GCTAAAAGGG GAGAGAGGAG ACCCTGGGAG CCCAGGAATC 
TCTCCTCCAG GTCCTCGTGG AGAAAAAGGT CCCCCAGGAC CCCCAGGGAG TTCAGGACCA 
CCTGGTCCTG CAGGTGCCAC AGGAAGAGCT CCTAAGGACA TTCCTGACCC GGGTCCACCT 
GGAGATCAGG GACCTCCTGG TCCTGATGGC CCAAGAGGAG CACCTGGGCC TCCAGGCCTC 
CCTGGGAGTG TTGACCTTCT GAGAGGGGAG CCAGGTGACT GTGGTCTACC AGGGCCACCA 
GGTCCCCCTG GCCCACCAGG CCCTCCAGGA TACAAAGGCT TTCCAGGATG TGATGGAAAA 
GATGGCCAGA AAGGACCAAT GGGATTCCCG GGACCGCAGG GACCACATGG ATTTCCTGGG 
CCACCTGGAG AGAAGGGTTT ACCTGGACCT CCAGGGAGAA AAGGGCCCAC TGGTCTTCCA 
GGTCCCAGAG GTGAACCGGG GCCACCTGCA GATGTGGATG ACTGTCCCCG AATCCCAGGC 
CTTCCTGGGG CGCCAGGCAT GAGAGGACCA GAAGGAGCCA TGGGGCTCTC TGGAATGAGA 
GGCCCCCCAG GACCAGCTCA TGGGTGTGTT CTGAGGACCA AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016806085.1
CDS	423..4667
Translation	MWSLHIVLMRCSFRLTKSLATDPWSLTLILFSVQYVYGSGKKYVGPCGGRDCSVCHCVPEKGSRGPPGPPGPQGPIGPLGAPGPIGLSGEKGMRGDHGPPGAAGDKGDKGSTGVPGFPGLDGIPGHPGPPGPRGKPGMSGHNGSRGDPGFPGGRGALGPGGPLGHPGEKGEKGNSVFILGAVKGIQGDRGDPGLPGLPGSWGAGGPAGPTGYPGEPGLVGPPGQPGRPGLKGNPGVGVKGQMGDPGEVGQQGSPGPTLLVEPPDFRLYKGEKGIKGIPGMVGLPGPPGRKGESDIGAKGEKGIPGFPGPRGDPGSYGSPGFPGLKGELGLVGDPGLFGLIGPKGDPGNRGHPGPPGVLVTPPLPLKGPPGDPGFPGRYGETGDVGPPGPPGLLGRPGEACAGMIGPPGPQGFPGLPGLPGEAGIPGRPDSAPGKPGKPGSPGLPGAPGLQGLPGSSVIYCSVGNPGPQGIKGKVGPPGGRGSKGEKGNEGLCACEPGPMGPPGPPGLPGRQGSKGDLGLPGWLGTKGDPGPPGAEGPPGLPGKHGASGPPGNKGAKGDMVVSRVKGHKGERGPDGPPGFPGQPGSHGRDGHAGEKGDPGPPGDHEDATPGGKGFPGPLGPPGKAGPLGPPGLGFPGPPGQRGHPGVPGHPGVRGPDGLKGQKGDTISCNVTYPGRHGPPGFDGPPGPKGFPGPQGAPGLSGSDGHKGRPGTPGTAEIPGPPGFRGDMGDPGFGGEKGSSPAGPPGPPGSPGVNGQKGIPGDPAFGHLGPPGKRGLSGVPGIKGPRGDPGCPGAEGPAGIPGFPGLKGPKGREGHAGFPGVPGPPGHSCERGAPGIPGQPGLPGYPGSPGAPGGKGQLGDVGPPGPAGMKGLPGLPGWPGAHGPPGLPGIPGPFGDDGLPGPPGPKGPQGLPGFPGFPGERGKPGAEGCPGTKGEPGEKGMSGLPGDRGLRGAKGAIGPPGDEGEMAIISQKGTPGEPGPPGDDGFPGERGDKGTPGMQGRRGEPGRYGPPGFQRGEPGEKGQPGHPGPPGPPGSTGLRGFIGFPGLPGDQGEPGSPGPPGFSGIDGARGPKGNKGDPASHFGPPGPKGEPGSPGCPGHFGASGEQGLPGIQGPRGSPGRPGPPGSSGPPGCPGDHGMPGLRGQPGEMGDPGPRGLQGDPGIPGPPGIKGPSGSPGLNGLHGLKGQKGTKGASGLHDVGPPGPVGIPGLKGERGDPGSPGISPPGPRGEKGPPGPPGSSGPPGPAGATGRAPKDIPDPGPPGDQGPPGPDGPRGAPGPPGLPGSVDLLRGEPGDCGLPGPPGPPGPPGPPGYKGFPGCDGKDGQKGPMGFPGPQGPHGFPGPPGEKGLPGPPGRKGPTGLPGPRGEPGPPADVDDCPRIPGLPGAPGMRGPEGAMGLSGMRGPPGPAHGCVLRTK

Target ORF information:

RefSeq Version	XM_016950596.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes collagen type IV alpha 4 (COL4A4), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016950596.1

ORF Insert Sequence:

ATGTGGTCTC TGCACATAGT ACTAATGAGG TGCTCCTTCA GATTGACCAA GTCCTTGGCC 
ACAGATCCCT GGTCACTTAC ACTCATTCTC TTTTCTGTAC AATATGTATA TGGGAGTGGA 
AAGAAATACG TTGGTCCTTG TGGAGGAAGA GATTGCTCTG TTTGCCACTG TGTTCCTGAA 
AAGGGGTCTC GGGGTCCACC AGGACCACCA GGGCCACAGG GTCCAATTGG ACCCCTGGGA 
GCCCCAGGAC CCATTGGGCT TTCAGGAGAG AAAGGAATGA GAGGGGACCA CGGCCCTCCT 
GGAGCAGCAG GGGACAAAGG AGATAAGGGT TCAACTGGTG TTCCTGGATT TCCAGGTTTA 
GATGGCATAC CTGGGCACCC AGGGCCTCCT GGACCCAGAG GCAAACCTGG TATGAGTGGC 
CACAATGGCT CAAGAGGTGA CCCAGGGTTT CCAGGAGGAA GAGGAGCTCT TGGCCCAGGA 
GGCCCCCTAG GCCATCCTGG GGAAAAGGGA GAAAAAGGAA ATTCAGTGTT CATTTTAGGT 
GCCGTTAAAG GTATTCAGGG AGACAGAGGG GACCCAGGAC TGCCTGGCTT ACCAGGATCT 
TGGGGGGCAG GAGGACCGGC GGGTCCCACA GGATATCCTG GAGAGCCAGG GTTAGTGGGA 
CCTCCGGGCC AACCAGGGCG TCCAGGTTTG AAGGGAAATC CCGGTGTGGG AGTAAAGGGG 
CAAATGGGAG ACCCGGGTGA GGTTGGTCAG CAAGGTTCTC CTGGACCCAC CCTGTTGGTG 
GAGCCACCTG ACTTTCGTCT CTATAAAGGA GAAAAGGGTA TAAAAGGAAT TCCTGGAATG 
GTTGGACTGC CAGGACCACC AGGACGCAAG GGAGAATCTG ATATTGGGGC AAAAGGAGAA 
AAAGGTATTC CTGGATTTCC AGGGCCTCGG GGGGATCCTG GTTCCTATGG ATCTCCAGGT 
TTTCCAGGAT TAAAGGGAGA ACTAGGACTG GTTGGAGATC CTGGGCTATT TGGATTAATT 
GGCCCAAAGG GGGATCCTGG AAATCGAGGG CACCCAGGAC CACCAGGTGT TTTGGTGACT 
CCACCTCTTC CACTCAAAGG CCCACCAGGG GACCCAGGGT TCCCTGGCCG CTATGGAGAA 
ACAGGGGATG TTGGACCACC TGGTCCCCCA GGTCTCTTGG GCAGACCAGG GGAAGCCTGT 
GCAGGCATGA TAGGACCCCC TGGGCCACAA GGATTTCCTG GTCTTCCTGG GCTTCCAGGA 
GAAGCTGGTA TTCCTGGGAG ACCTGATTCT GCTCCAGGAA AACCAGGGAA GCCAGGATCA 
CCTGGCTTGC CTGGAGCACC AGGCCTGCAG GGCCTCCCAG GATCAAGTGT GATATACTGT 
AGTGTTGGGA ATCCTGGACC ACAAGGAATA AAAGGCAAAG TGGGTCCCCC AGGAGGAAGA 
GGCTCAAAAG GAGAAAAAGG AAATGAAGGA CTCTGTGCCT GTGAGCCTGG ACCCATGGGC 
CCCCCTGGCC CTCCAGGACT TCCTGGGAGG CAGGGGAGTA AGGGAGACTT GGGGCTCCCT 
GGCTGGCTTG GAACAAAAGG TGACCCAGGA CCTCCTGGTG CTGAAGGACC TCCAGGGCTA 
CCAGGAAAGC ATGGTGCCTC TGGACCACCT GGCAACAAAG GGGCGAAGGG TGACATGGTT 
GTATCAAGAG TTAAAGGGCA CAAAGGAGAA AGAGGTCCTG ATGGGCCCCC AGGATTTCCA 
GGGCAGCCAG GATCACATGG TCGGGATGGA CATGCTGGAG AAAAAGGGGA TCCAGGACCC 
CCAGGGGATC ATGAAGATGC GACCCCAGGT GGTAAAGGAT TTCCTGGACC TCTGGGCCCC 
CCGGGCAAAG CAGGACCTTT GGGGCCCCCA GGACTGGGAT TTCCTGGTCC ACCAGGACAG 
CGAGGCCACC CAGGAGTTCC AGGCCACCCA GGTGTGAGGG GCCCTGATGG CTTGAAGGGT 
CAGAAAGGTG ACACAATTTC TTGCAACGTA ACCTACCCTG GGAGGCACGG CCCTCCAGGT 
TTTGATGGAC CTCCAGGTCC GAAGGGATTT CCAGGTCCCC AAGGTGCCCC TGGGCTGAGT 
GGTTCAGATG GGCATAAAGG CAGACCTGGC ACACCAGGAA CAGCGGAAAT ACCAGGTCCA 
CCTGGTTTTC GTGGTGACAT GGGAGATCCG GGTTTTGGAG GTGAAAAGGG GTCCTCCCCT 
GCTGGGCCCC CAGGCCCTCC CGGCTCACCA GGAGTGAATG GTCAGAAAGG AATCCCGGGA 
GACCCTGCAT TTGGTCACCT GGGACCCCCG GGAAAGAGGG GTCTTTCAGG AGTGCCAGGG 
ATAAAAGGAC CCAGAGGTGA TCCGGGATGT CCAGGGGCTG AAGGGCCAGC TGGCATTCCT 
GGATTCCCAG GTCTCAAAGG TCCCAAAGGC AGAGAGGGAC ATGCTGGGTT TCCAGGTGTC 
CCAGGTCCAC CTGGCCATTC CTGTGAAAGA GGTGCTCCAG GGATACCAGG GCAACCGGGA 
CTCCCTGGGT ATCCAGGTAG CCCAGGTGCT CCAGGTGGGA AAGGACAGCT GGGAGATGTG 
GGGCCTCCCG GGCCAGCTGG AATGAAAGGC CTCCCTGGAC TCCCAGGATG GCCTGGGGCA 
CATGGTCCCC CAGGCCTCCC AGGAATCCCA GGTCCCTTTG GAGATGATGG GCTACCTGGT 
CCTCCAGGTC CAAAGGGACC CCAGGGGCTG CCTGGTTTCC CAGGTTTTCC CGGAGAAAGA 
GGAAAGCCTG GTGCAGAGGG ATGTCCTGGC ACAAAGGGAG AACCTGGAGA GAAGGGCATG 
TCTGGCCTTC CCGGAGACCG GGGACTGAGA GGGGCCAAAG GAGCCATAGG ACCTCCCGGA 
GATGAAGGAG AAATGGCTAT CATTTCCCAA AAGGGAACAC CTGGGGAACC TGGACCTCCT 
GGAGATGATG GATTCCCAGG AGAAAGAGGT GATAAAGGAA CTCCCGGGAT GCAAGGGAGA 
AGAGGAGAGC CGGGAAGATA CGGACCACCT GGATTTCAGA GAGGGGAACC TGGTGAGAAA 
GGTCAGCCAG GGCATCCTGG ACCCCCAGGC CCTCCAGGCT CAACTGGTCT AAGAGGGTTC 
ATTGGTTTTC CAGGACTTCC AGGTGACCAG GGTGAGCCAG GTTCTCCAGG TCCCCCTGGA 
TTTTCAGGAA TTGATGGAGC AAGAGGACCT AAAGGAAACA AAGGTGACCC TGCCAGTCAC 
TTTGGTCCAC CTGGTCCAAA GGGTGAGCCA GGTAGCCCTG GATGTCCAGG GCATTTTGGA 
GCATCTGGAG AGCAGGGCTT GCCTGGTATT CAAGGGCCCA GAGGATCACC TGGAAGGCCA 
GGGCCACCTG GCTCCTCTGG ACCACCAGGG TGCCCAGGTG ATCACGGGAT GCCTGGGCTG 
AGGGGACAGC CAGGAGAAAT GGGAGACCCT GGGCCAAGAG GCCTCCAGGG GGATCCAGGG 
ATACCAGGTC CTCCGGGAAT AAAAGGTCCC TCCGGATCAC CTGGCCTGAA CGGCTTGCAT 
GGATTGAAAG GTCAGAAAGG AACTAAAGGT GCTTCAGGTT TGCATGATGT GGGGCCACCT 
GGTCCAGTGG GAATACCTGG GCTAAAAGGG GAGAGAGGAG ACCCTGGGAG CCCAGGAATC 
TCTCCTCCAG GTCCTCGTGG AGAAAAAGGT CCCCCAGGAC CCCCAGGGAG TTCAGGACCA 
CCTGGTCCTG CAGGTGCCAC AGGAAGAGCT CCTAAGGACA TTCCTGACCC GGGTCCACCT 
GGAGATCAGG GACCTCCTGG TCCTGATGGC CCAAGAGGAG CACCTGGGCC TCCAGGCCTC 
CCTGGGAGTG TTGACCTTCT GAGAGGGGAG CCAGGTGACT GTGGTCTACC AGGGCCACCA 
GGTCCCCCTG GCCCACCAGG CCCTCCAGGA TACAAAGGCT TTCCAGGATG TGATGGAAAA 
GATGGCCAGA AAGGACCAAT GGGATTCCCG GGACCGCAGG GACCACATGG ATTTCCTGGG 
CCACCTGGAG AGAAGGGTTT ACCTGGACCT CCAGGGAGAA AAGGGCCCAC TGGTCTTCCA 
GGTCCCAGAG GTGAACCGGG GCCACCTGCA GATGTGGATG ACTGTCCCCG AATCCCAGGC 
CTTCCTGGGG CGCCAGGCAT GAGAGGACCA GAAGGAGCCA TGGGGCTCTC TGGAATGAGA 
GGCCCCCCAG GACCAGCTCA TGGGTGTGTT CTGAGGACCA AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	COL4A4
Entrez Gene ID	459986
Full Name	collagen type IV alpha 4 chain
General protein information	Preferred Names collagen type IV alpha 4 chain Names collagen alpha-4(IV) chain alpha 4 type IV collagen collagen, type IV, alpha 4
Gene Type	protein-coding
Organism	Pan troglodytes(chimpanzee)

Pan troglodytes (chimpanzee)	COL4A4	XP_516128.2
	COL4A4	XP_001110249.1
Canis lupus familiaris (dog)	COL4A4	NP_001026988.1
Homo sapiens (human)	COL4A4	NP_000083.3
Mus musculus (house mouse)	Col4a4	NP_031761.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

COL4A4 cDNA ORF clone, Pan troglodytes(chimpanzee)

Gene

Clones

gRNAs

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