NOTCH2 gene cDNA ORF clone, Pan troglodytes(chimpanzee)

mRNA	Protein	Name
XM_024354929.1	XP_024210697.1	neurogenic locus notch homolog protein 2 isoform X3
XM_016924542.1	XP_016780031.1	neurogenic locus notch homolog protein 2 isoform X3
XM_016924527.1	XP_016780016.1	neurogenic locus notch homolog protein 2 isoform X1
XM_024354928.1	XP_024210696.1	neurogenic locus notch homolog protein 2 isoform X2
XM_016924554.1	XP_016780043.1	neurogenic locus notch homolog protein 2 isoform X4
XM_016924533.1	XP_016780022.1	neurogenic locus notch homolog protein 2 isoform X2
XM_016924549.1	XP_016780038.1	neurogenic locus notch homolog protein 2 isoform X3
XM_024354924.1	XP_024210692.1	neurogenic locus notch homolog protein 2 isoform X1
XM_024354927.1	XP_024210695.1	neurogenic locus notch homolog protein 2 isoform X2

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OPc92102	XM_024354929.1 Latest version!	Pan troglodytes notch 2 (NOTCH2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OPc59961	XM_016924533.1 Latest version!	Pan troglodytes notch 2 (NOTCH2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
OPc59962	XM_016924542.1 Latest version!	Pan troglodytes notch 2 (NOTCH2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
OPc59962	XM_016924549.1 Latest version!	Pan troglodytes notch 2 (NOTCH2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
OPc92100	XM_024354924.1 Latest version!	Pan troglodytes notch 2 (NOTCH2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OPc92101	XM_024354927.1 Latest version!	Pan troglodytes notch 2 (NOTCH2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OPc92101	XM_024354928.1 Latest version!	Pan troglodytes notch 2 (NOTCH2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OPc59960	XM_016924527.1 Latest version!	Pan troglodytes notch 2 (NOTCH2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
OPc59963	XM_016924554.1 Latest version!	Pan troglodytes notch 2 (NOTCH2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$258.30 $369.00

NOTCH2 ( XM_024354929.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_024210697.1 Pan troglodytes notch 2 (NOTCH2), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OPc92102
Clone ID Related Accession (Same CDS sequence)	XM_024354929.1
Accession Version	XM_024354929.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 828bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	neurogenic locus notch homolog protein 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036879.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGGAC ATCAAAGATC TGAAGGCCCA GCTGCAGAAT GCCAACAAGG TCATTCAGAA 
CCTCAAGAGC CAGCATTGCA GTGTCGAGAT GGCTATGAAC CCTGTGTAAA TGAAGGAATG 
TGTGTTACCT ACCACAATGG CACAGGATAC TGCAAATGTC CAGAAGGCTT CTTGGGGGAA 
TATTGTCAAC ATCGAGACCC CTGTGAGAAG AACCGCTGCC AGAATGGTGG GACTTGTGTG 
GCCCAGGCCA TGCTGGGGAA AGCCACGTGC CGATGTGCCT CAGGGTTTAC AGGAGAGGAC 
TGCCAGTACT CGACATCTCA TCCATGCTTT GTGTCTCGAC CCTGCCTGAA TGGCGGCACA 
TGCCATATGC TCAGCCGGGA TACCTATGAG TGCACCTGTC AAGTCGGGTT TACAGGTAAG 
GAGTGCCAAT GGACCGATGC CTGCCTGTCT CATCCCTGTG CAAATGGAAG TACCTGTACC 
ACTGTGGCCA ACCAGTTCTC CTGCAAATGC CTCACAGGCT TCACAGGGCA GAAGTGTGAG 
ACTGATGTCA ATGAGTGTGA CATTCCAGGA CACTGCCAGC ATGGTGGCAC CTGCCTCAAC 
CTGCCTGGTT CCTACCAGTG CCAGTGCCCT CAGGGCTTCA CAGGCCAGTA CTGTGACAGC 
CTGTATGTGC CCTGTGCACC CTCGCCTTGT GTCAATGGAG GCACCTGTCG GCAGACTGGT 
GACTTCACTT TTGAGTGCAG CTGCCTTCCA GAAACAGAAA TAAGAGGAAC AGAGCTCTGG 
GAAAGAGACA GGCAAGTCTG GAATGGAAAA GAACACGATG AGAATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024210697.1
CDS	145..972
Translation	MKGHQRSEGPAAECQQGHSEPQEPALQCRDGYEPCVNEGMCVTYHNGTGYCKCPEGFLGEYCQHRDPCEKNRCQNGGTCVAQAMLGKATCRCASGFTGEDCQYSTSHPCFVSRPCLNGGTCHMLSRDTYECTCQVGFTGKECQWTDACLSHPCANGSTCTTVANQFSCKCLTGFTGQKCETDVNECDIPGHCQHGGTCLNLPGSYQCQCPQGFTGQYCDSLYVPCAPSPCVNGGTCRQTGDFTFECSCLPETEIRGTELWERDRQVWNGKEHDEN

Target ORF information:

RefSeq Version	XM_024354929.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes notch 2 (NOTCH2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024354929.1

ORF Insert Sequence:

ATGAAAGGAC ATCAAAGATC TGAAGGCCCA GCTGCAGAAT GCCAACAAGG TCATTCAGAA 
CCTCAAGAGC CAGCATTGCA GTGTCGAGAT GGCTATGAAC CCTGTGTAAA TGAAGGAATG 
TGTGTTACCT ACCACAATGG CACAGGATAC TGCAAATGTC CAGAAGGCTT CTTGGGGGAA 
TATTGTCAAC ATCGAGACCC CTGTGAGAAG AACCGCTGCC AGAATGGTGG GACTTGTGTG 
GCCCAGGCCA TGCTGGGGAA AGCCACGTGC CGATGTGCCT CAGGGTTTAC AGGAGAGGAC 
TGCCAGTACT CGACATCTCA TCCATGCTTT GTGTCTCGAC CCTGCCTGAA TGGCGGCACA 
TGCCATATGC TCAGCCGGGA TACCTATGAG TGCACCTGTC AAGTCGGGTT TACAGGTAAG 
GAGTGCCAAT GGACCGATGC CTGCCTGTCT CATCCCTGTG CAAATGGAAG TACCTGTACC 
ACTGTGGCCA ACCAGTTCTC CTGCAAATGC CTCACAGGCT TCACAGGGCA GAAGTGTGAG 
ACTGATGTCA ATGAGTGTGA CATTCCAGGA CACTGCCAGC ATGGTGGCAC CTGCCTCAAC 
CTGCCTGGTT CCTACCAGTG CCAGTGCCCT CAGGGCTTCA CAGGCCAGTA CTGTGACAGC 
CTGTATGTGC CCTGTGCACC CTCGCCTTGT GTCAATGGAG GCACCTGTCG GCAGACTGGT 
GACTTCACTT TTGAGTGCAG CTGCCTTCCA GAAACAGAAA TAAGAGGAAC AGAGCTCTGG 
GAAAGAGACA GGCAAGTCTG GAATGGAAAA GAACACGATG AGAATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NOTCH2 ( XM_016924533.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016780022.1 Pan troglodytes notch 2 (NOTCH2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OPc59961
Clone ID Related Accession (Same CDS sequence)	XM_016924533.1
Accession Version	XM_016924533.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7299bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	neurogenic locus notch homolog protein 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006468.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTGTTA CCTACCACAA TGGCACAGGA TACTGCAAAT GTCCAGAAGG CTTCTTGGGG 
GAATATTGTC AACATCGAGA CCCCTGTGAG AAGAACCGCT GCCAGAATGG TGGGACTTGT 
GTGGCCCAGG CCATGCTGGG GAAAGCCATG TGCCGATGTG CCTCAGGGTT TACAGGAGAG 
GACTGCCAGT ACTCGACATC TCATCCATGC TTTGTGTCTC GACCCTGCCT GAATGGCGGC 
ACATGCCATA TGCTCAGCCG GGATACCTAT GAGTGCACCT GTCAAGTCGG GTTTACAGGT 
AAGGAGTGCC AATGGACCGA TGCCTGCCTG TCTCATCCCT GTGCAAATGG AAGTACCTGT 
ACCACTGTGG CCAACCAGTT CTCCTGCAAA TGCCTCACAG GCTTCACAGG GCAGAAGTGT 
GAGACTGATG TCAATGAGTG TGACATTCCA GGACACTGCC AGCATGGTGG CACCTGCCTC 
AACCTGCCTG GTTCCTACCA GTGCCAGTGC CCTCAGGGCT TCACAGGCCA GTACTGTGAC 
AGCCTGTATG TGCCCTGTGC ACCCTCGCCT TGTGTCAATG GAGGCACCTG TCGGCAGACT 
GGTGACTTCA CTTTTGAGTG CAACTGCCTT CCAGGTTTTG AAGGGAGCAC CTGTGAGAGG 
AATATTGATG ACTGCCCTAA CCACAGGTGT CAGAATGGAG GGGTTTGTGT GGATGGGGTC 
AACACTTACA ACTGCCGCTG TCCCCCACAA TGGACAGGAC AGTTCTGCAC AGAGGATGTG 
GATGAATGCC TGCTGCAGCC CAATGCCTGT CAAAATGGGG GCACCTGTGC CAACCGCAAT 
GGAGGCTATG GCTGTGTATG TGTCAACGGC TGGAGTGGAG ATGACTGCAG TGAGAACATT 
GATGATTGTG CCTTCGCCTC CTGTACTCCA GGCTCCACCT GCATCGACCG TGTGGCCTCC 
TTCTCTTGCA TGTGCCCAGA GGGGAAGGCA GGTCTCCTGT GTCATCTGGA TGATGCATGC 
ATCAGCAATC CTTGCCACAA GGGGGCACTG TGTGACACCA ACCCCCTAAA TGGGCAATAT 
ATTTGCACCT GCCCACAAGG CTACAAAGGG GCTGACTGCA CAGAAGATGT GGATGAATGT 
TCCATGGCCA ATAGCAATCC TTGTGAGCAT GCAGGAAAAT GTGTGAACAC GGATGGCGCC 
TTCCACTGTG AGTGTCTGAA GGGTTATGCA GGACCTCGTT GTGAGATGGA CATCAATGAG 
TGCCATTCAG ACCCCTGCCA GAATGATGCT ACCTGTCTGG ATAAGATTGG AGGCTTCACA 
TGTCTGTGCA TGCCAGGTTT CAAAGGTGTG CATTGTGAAT TAGAAATAAA TGAATGTCAG 
AGCAACCCTT GTGTGAACAA TGGGCAGTGT GTGGATAAAG TCAATCGTTT CCAGTGCCTG 
TGTCCTCCTG GTTTCACTGG GCCAGTTTGC CAGATTGATA TTGATGACTG TTCCAGTACT 
CCGTGTCTGA ATGGGGCAAA GTGTATCGAT CACCCGAATG GCTATGAATG CCAGTGTGCC 
ACAGGTTTCA CTGGTGTGTT GTGTGAGGAG AACATTGACA ACTGTGACCC CGATCCTTGC 
CACCATGGTC AGTGTCAGGA TGGTATTGAT TCCTACACCT GCATCTGCAA TCCCGGGTAC 
ATGGGCGCCA TCTGCAGTGA CCAGATTGAT GAATGTTACA GCAGCCCTTG CCTGAACGAT 
GGTCGCTGCA TTGACCTGGT CAATGGCTAC CAGTGCAACT GCCAGCCGGG CACGTCAGGG 
GTTAATTGTG AAATTAATTT TGATGACTGT GCAAGTAACC CTTGTATCCA TGGAAACTGT 
ATGGATGGCA TTAATCGCTA CAGTTGTGTC TGCTCACCAG GATTCACAGG GCAGAGATGT 
AACATTGACA TTGATGAGTG TGCCTCCAAT CCCTGTCGCA AGGGTGCAAC ATGTATCAAC 
GGTGTGAATG GTTTCCGCTG TATATGCCCC GAGGGACCCC ATCACCCCAG CTGCTACTCA 
CAGGTGAACG AATGCCTGAG CAATCCCTGC ATCCATGGAA ACTGTACTGG AGGTCTCAGT 
GGATATAAGT GTCTCTGTGA TGCAGGCTGG GTTGGCATCA ACTGTGAAGT GGACAAAAAT 
GAATGCCTTT CGAATCCATG CCAGAATGGA GGAACTTGTG ACAATCTGGT GAATGGATAC 
AGGTGTACTT GCAAGAAGGG CTTTAAAGGC TATAACTGCC AGGTGAATAT TGATGAATGT 
GCCTCAAATC CATGCCTGAA CCAAGGAACC TGCTTTGATG ACATAAGTGG CTACACTTGC 
CACTGTGTGC TGCCATACAC AGGCAAGAAT TGTCAGACAG TATTGGCTCC CTGTTCCCCA 
AACCCTTGTG AGAATGCTGC TGTTTGCAAA GAGTCACCAA ATTTTGAGAG TTATACTTGC 
TTGTGTGCTC CTGGCTGGCA AGGTCAGCGG TGTACCATTG ACATTGACGA GTGTATCTCC 
AAGCCCTGCA TGAACCATGG TCTCTGCCAT AACACCCAGG GCAGCTACAT GTGTGAATGT 
CCACCAGGCT TCAGTGGTAT GGACTGTGAG GAGGACATTG ATGACTGCCT TGCCAATCCT 
TGCCAGAATG GAGGTTCCTG TGTGGATGGA GTGAATACTT TCTCCTGCCT CTGCCTTCCG 
GGTTTCACTG GGGATAAGTG CCAGACAGAC ATGAATGAGT GTCTGAGTGA ACCCTGTAAG 
AATGGAGGGA CCTGCTCTGA CTACGTCAAC AGTTACACTT GCAAGTGCCA GGCAGGATTT 
GATGGAGTCC ATTGTGAGAA CAACATCAAT GAGTGCACTG AGAGCTCCTG TTTCAATGGT 
GGCACATGTG TTGATGGGAT TAACTCCTTC TCTTGCTTGT GCCCTGTGGG TTTCACTGGA 
TCCTTCTGCC TCCATGAGAT CAATGAATGC AGCTCTCATC CATGCCTCAA TGAGGGAACG 
TGTGTTGATG GCCTGGGTAC CTACCGCTGC AGCTGCCCCC TGGGCTACAC TGGGAAAAAC 
TGTCAGACCC TGGTGAACCT CTGCAGTCGG TCTCCATGTA AAAACAAAGG TACTTGCGTT 
CAGAAAAAAG CAGAGTCCCA GTGCCTATGT CCATCTGGAT GGGCTGGTGC CTATTGTGAC 
GTGCCCAATG TCTCTTGTGA CATAGCAGCC TCCAGGAGAG GTGTGCTTGT TGAACACTTG 
TGCCAGCACT CAGGTGTCTG CATCAATGCT GGCAACACGC ATTACTGTCA GTGCCCCCTG 
GGCTATACTG GGAGCTACTG TGAGGAGCAA CTCGATGAGT GTGCGTCCAA CCCCTGCCAG 
CACGGGGCAA CATGCAGTGA CTTCATTGGT GGATACAGAT GTGAGTGTGT CCCAGGCTAT 
CAGGGTGTCA ACTGTGAGTA TGAAGTGGAT GAGTGCCAGA ATCAGCCCTG CCAGAATGGA 
GGCACCTGTA TTGACCTTGT GAACCATTTC AAGTGCTCTT GCCCACCAGG CACTCGGGGC 
CTACTCTGTG AAGAGAACAT TGATGACTGT GCCCGGGGTC CCCATTGCCT TAATGGTGGT 
CAGTGCATGG ATAGGATTGG AGGCTACAGT TGTCGCTGCT TGCCTGGCTT TGCTGGGGAG 
CGTTGTGAGG GAGACATCAA CGAGTGCCTC TCCAACCCCT GCAGCTCTGA GGGCAGCCTG 
GACTGTATAC AGCTCACCAA TGACTACCTG TGTGTTTGCC GTAGTGCCTT TACTGGCCGG 
CACTGTGAAA CCTTCGTCGA TGTGTGTCCC CAGATGCCCT GCCTGAATGG AGGGACTTGT 
GCTGTGGCCA GTAACATGCC TGATGGTTTC ATTTGCCGTT GTCCCCCGGG ATTTTCTGGG 
GCAAGGTGCC AGAGCAGCTG TGGACAAGTG AAATGTAGGA AAGGGGAGCA GTGTGTGCAC 
ACCGCCTCTG GACCCCGCTG CTTCTGCCCC AGTCCCCGGG ACTGCGAGTC AGGCTGTGCC 
AGTAGCCCCT GCCAGCACGG GGGCAGCTGC CACCCTCAGC GCCAGCCTCC TTATTACTCC 
TGCCAGTGTG CCCCACCATT CTCGGGTAGC CGCTGTGAAC TCTACACGGC ACCCCCCAGC 
ACCCCTCCTG CCACCTGTCT GAGCCAGTAT TGTGCCGACA AAGCTCGGGA TGGCGTCTGT 
GATGAGGCCT GCAACAGCCA TGCCTGCCAG TGGGATGGGG GTGACTGTTC TCTCACCATG 
GAGAACCCCT GGGCCAACTG CTCCTCCCCA CTTCCCTGCT GGGATTATAT CAACAACCAG 
TGTGATGAGC TGTGCAACAC GGTCGAGTGC CTGTTTGACA ACTTTGAATG CCAGGGGAAC 
AGCAAGACAT GCAAGTATGA CAAATACTGT GCAGACCACT TCAAAGACAA CCACTGTGAC 
CAGGGGTGCA ACAGTGAGGA GTGTGGTTGG GATGGGCTGG ACTGTGCTGC TGACCAACCT 
GAGAACCTGG CAGAAGGTAC CCTGGTTATT GTGGTATTGA TGCCACCTGA ACAACTGCTC 
CAGGATGCTC GCAGCTTCTT GCGGGCACTG GGTACCCTGC TGCACACCAA CCTGCGCATT 
AAGCGGGACT CCCAGGGGGA ACTCATGGTG TACCCCTATT ATGGTGAGAA GTCAGCTGCT 
ATGAAGAAAC AGAGGATGAC ACGCAGGTCC CTTCCTGGTG AACAAGAACA GGAGGTGGCT 
GGCTCTAAAG TCTTTCTGGA AATTGACAAC CGCCAGTGTG TTCAAGACTC AGACCACTGC 
TTCAAGAACA CGGATGCAGC AGCAGCTCTC CTGGCCTCTC ACGCCATACA GGGGACCCTG 
TCATACCCTC TTGTGTCTGT CGTCAGTGAA TCCCTGACTC CAGAACGCAC TCAGCTCCTC 
TATCTCCTTG CTGTTGCTGT TGTCATCATT CTGTTTATTA TTCTGCTGGG GGTAATCATG 
GCAAAACGAA AGCGTAAGCA TGGCTCTCTC TGGCTGCCTG AAGGTTTCAC TCTTCGCCGA 
GATGCAAGCA ATCACAAGCG TCGTGAGCCA GTGGGACAGG ATGCTGTGGG GCTAAAAAAT 
CTCTCAGTGC AAGTCTCAGA AGCTAACCTA ATTGGTTCTG GAACAAGTGA ACACTGGGTC 
GATGATGAAG GGCCCCAGCC AAAGAAAGTA AAGGCTGAAG ATGAGGCCTT ACTCTCAGAA 
GAAGATGACC CCATTGATCG ACGGCCATGG ACACAGCAGC ACCTTGAAGC CGCAGACATC 
CGTAGGACAC CATCGCTGGC TCTCACCCCT CCTCAGGCAG AGCAGGAGGT GGATGTGTTA 
GATGTGAATG TCCGTGGCCC AGATGGCTGC ACCCCATTGA TGTTGGCTTC TCTCCGAGGA 
GGCAGCTCAG ATTTGAGTGA TGAAGATGAA GATGCAGAGG ACTCTTCTGC TAACATCATC 
ACAGACTTGG TCTACCAGGG TGCCAGCCTC CAGGCCCAGA CAGACCGGAC TGGTGAGATG 
GCCCTGCACC TTGCAGCCCG CTACTCACGG GCTGATGCTG CCAAGCGTCT CCTGGATGCA 
GGTGCAGATG CCAATGCCCA GGACAACATG GGCCGCTGCC CACTCCATGC TGCAGTGGCA 
GCTGATGCCC AAGGTGTCTT CCAGATTCTG ATTCGCAACC GAGTAACTGA TCTAGATGCC 
AGGATGAATG ATGGTACTAC ACCCCTGATC CTGGCTGCCC GCCTGGCTGT GGAGGGAATG 
GTGGCAGAAC TGATCAACTG CCAAGCGGAT GTGAATGCAG TGGATGACCA TGGAAAATCT 
GCTCTTCACT GGGCAGCTGC TGTCAATAAT GTGGAGGCAA CTCTTTTGTT GTTGAAAAAT 
GGAGCCAACC GAGACATGCA GGACAACAAG GAAGAGACAC CTCTGTTTCT TGCTGCCCGG 
GAGGGGAGCT ATGAAGCAGC CAAGATCCTG TTAGACCATT TTGCCAATCG AGACATCACA 
GACCATATGG ATCGTCTTCC CCGGGATGTG GCTCGGGATC GCATGCACCA TGACATTGTG 
CGCCTTCTGG ATGAATACAA TGTGACCCCA AGCCCTCCAG GCACCGTGTT GACTTCTGCT 
CTCTCACCTG TCATCTGTGG GCCCAACAGA TCTTTCCTCA GCCTGAAGCA CACCCCAATG 
GGCAAGAAGT CTAGACGGCC CAGTGCCAAG AGTACCATGC CTACTAGCCT CCCTAACCTT 
GCCAAGGAGG CAAAGGATGC CAAGGGTAGT AGGAGGAAGA AGTCTCTGAG TGAGAAGGTC 
CAACTGTCTG AGAGTTCAGT AACTTTATCC CCTGTTGATT CCCTAGAATC TCCTCACACG 
TATGTTTCCG ACACCACATC CTCTCCAATG ATTACATCCC CTGGGATCTT ACAGGCCTCA 
CCCAACCCTA TGTTGGCCAC TGCCGCCCCT CCTGCCCCAG TCCATGCCCA GCATGCACTA 
TCTTTTTCTA ACCTTCATGA AATGCAGCCT TTGGCACATG GGGCCAGCAC TGTGCTTCCC 
TCAGTGAGCC AGTTGCTATC CCACCACCAC ATTGTGTCTC CAGGCAGTGG CAGTGCTGGA 
AGCTTGAGTA GGCTCCATCC AGTCCCAGTC CCAGCAGATT GGATGAACCG CATGGAGGTG 
AATGAGACCC AGTACAATGA GATGTTTGGT ATGGTCCTGG CTCCAGCTGA GGGCACCCAT 
CCTGGCATAG CTCCCCAGAG CAGGCCACCT GAAGGGAAGC ACATAACCAC CCCTCGGGAG 
CCCTTGCCCC CCATTGTGAC TTTCCAGCTC ATCCCTAAAG GCAGTATTGC CCAACCAGCG 
GGGGCTCCCC AGCCTCAGTC CACCTGCCCT CCAGCTGTTG CGGGCCCCCT GCCCACCATG 
TACCAGATTC CAGAAATGGC CCGTTTGCCC AGTGTGGCTT TCCCCACTGC CATGATGCCC 
CAGCAGGACG GGCAGGTAGC TCAGACCATT CTCCCAGCCT ATCATCCTTT CCCAGCCTCT 
GTGGGCAAGT ACCCCACACC CCCTTCACAG CACAGTTATG CTTCCTCAAA TGCTGCTGAG 
CGAACACCCA GTCACAGTGG TCACCTCCAG GGTGAGCATC CCTACCTGAC ACCATCCCCA 
GAGTCTCCTG ACCAGTGGTC AAGTTCATCA CCCCACTCTG CTTCTGACTG GTCAGATGTG 
ACCACCAGCC CTACCCCTGG GGGTGCTGGA GGAGGTCAGC GGGGACCTGG GACACACATG 
TCTGAGCCAC CACACAACAA CATGCAGGTT TATGCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016780022.1
CDS	381..7679
Translation	MCVTYHNGTGYCKCPEGFLGEYCQHRDPCEKNRCQNGGTCVAQAMLGKAMCRCASGFTGEDCQYSTSHPCFVSRPCLNGGTCHMLSRDTYECTCQVGFTGKECQWTDACLSHPCANGSTCTTVANQFSCKCLTGFTGQKCETDVNECDIPGHCQHGGTCLNLPGSYQCQCPQGFTGQYCDSLYVPCAPSPCVNGGTCRQTGDFTFECNCLPGFEGSTCERNIDDCPNHRCQNGGVCVDGVNTYNCRCPPQWTGQFCTEDVDECLLQPNACQNGGTCANRNGGYGCVCVNGWSGDDCSENIDDCAFASCTPGSTCIDRVASFSCMCPEGKAGLLCHLDDACISNPCHKGALCDTNPLNGQYICTCPQGYKGADCTEDVDECSMANSNPCEHAGKCVNTDGAFHCECLKGYAGPRCEMDINECHSDPCQNDATCLDKIGGFTCLCMPGFKGVHCELEINECQSNPCVNNGQCVDKVNRFQCLCPPGFTGPVCQIDIDDCSSTPCLNGAKCIDHPNGYECQCATGFTGVLCEENIDNCDPDPCHHGQCQDGIDSYTCICNPGYMGAICSDQIDECYSSPCLNDGRCIDLVNGYQCNCQPGTSGVNCEINFDDCASNPCIHGNCMDGINRYSCVCSPGFTGQRCNIDIDECASNPCRKGATCINGVNGFRCICPEGPHHPSCYSQVNECLSNPCIHGNCTGGLSGYKCLCDAGWVGINCEVDKNECLSNPCQNGGTCDNLVNGYRCTCKKGFKGYNCQVNIDECASNPCLNQGTCFDDISGYTCHCVLPYTGKNCQTVLAPCSPNPCENAAVCKESPNFESYTCLCAPGWQGQRCTIDIDECISKPCMNHGLCHNTQGSYMCECPPGFSGMDCEEDIDDCLANPCQNGGSCVDGVNTFSCLCLPGFTGDKCQTDMNECLSEPCKNGGTCSDYVNSYTCKCQAGFDGVHCENNINECTESSCFNGGTCVDGINSFSCLCPVGFTGSFCLHEINECSSHPCLNEGTCVDGLGTYRCSCPLGYTGKNCQTLVNLCSRSPCKNKGTCVQKKAESQCLCPSGWAGAYCDVPNVSCDIAASRRGVLVEHLCQHSGVCINAGNTHYCQCPLGYTGSYCEEQLDECASNPCQHGATCSDFIGGYRCECVPGYQGVNCEYEVDECQNQPCQNGGTCIDLVNHFKCSCPPGTRGLLCEENIDDCARGPHCLNGGQCMDRIGGYSCRCLPGFAGERCEGDINECLSNPCSSEGSLDCIQLTNDYLCVCRSAFTGRHCETFVDVCPQMPCLNGGTCAVASNMPDGFICRCPPGFSGARCQSSCGQVKCRKGEQCVHTASGPRCFCPSPRDCESGCASSPCQHGGSCHPQRQPPYYSCQCAPPFSGSRCELYTAPPSTPPATCLSQYCADKARDGVCDEACNSHACQWDGGDCSLTMENPWANCSSPLPCWDYINNQCDELCNTVECLFDNFECQGNSKTCKYDKYCADHFKDNHCDQGCNSEECGWDGLDCAADQPENLAEGTLVIVVLMPPEQLLQDARSFLRALGTLLHTNLRIKRDSQGELMVYPYYGEKSAAMKKQRMTRRSLPGEQEQEVAGSKVFLEIDNRQCVQDSDHCFKNTDAAAALLASHAIQGTLSYPLVSVVSESLTPERTQLLYLLAVAVVIILFIILLGVIMAKRKRKHGSLWLPEGFTLRRDASNHKRREPVGQDAVGLKNLSVQVSEANLIGSGTSEHWVDDEGPQPKKVKAEDEALLSEEDDPIDRRPWTQQHLEAADIRRTPSLALTPPQAEQEVDVLDVNVRGPDGCTPLMLASLRGGSSDLSDEDEDAEDSSANIITDLVYQGASLQAQTDRTGEMALHLAARYSRADAAKRLLDAGADANAQDNMGRCPLHAAVAADAQGVFQILIRNRVTDLDARMNDGTTPLILAARLAVEGMVAELINCQADVNAVDDHGKSALHWAAAVNNVEATLLLLKNGANRDMQDNKEETPLFLAAREGSYEAAKILLDHFANRDITDHMDRLPRDVARDRMHHDIVRLLDEYNVTPSPPGTVLTSALSPVICGPNRSFLSLKHTPMGKKSRRPSAKSTMPTSLPNLAKEAKDAKGSRRKKSLSEKVQLSESSVTLSPVDSLESPHTYVSDTTSSPMITSPGILQASPNPMLATAAPPAPVHAQHALSFSNLHEMQPLAHGASTVLPSVSQLLSHHHIVSPGSGSAGSLSRLHPVPVPADWMNRMEVNETQYNEMFGMVLAPAEGTHPGIAPQSRPPEGKHITTPREPLPPIVTFQLIPKGSIAQPAGAPQPQSTCPPAVAGPLPTMYQIPEMARLPSVAFPTAMMPQQDGQVAQTILPAYHPFPASVGKYPTPPSQHSYASSNAAERTPSHSGHLQGEHPYLTPSPESPDQWSSSSPHSASDWSDVTTSPTPGGAGGGQRGPGTHMSEPPHNNMQVYA

Target ORF information:

RefSeq Version	XM_016924533.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes notch 2 (NOTCH2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016924533.1

ORF Insert Sequence:

ATGTGTGTTA CCTACCACAA TGGCACAGGA TACTGCAAAT GTCCAGAAGG CTTCTTGGGG 
GAATATTGTC AACATCGAGA CCCCTGTGAG AAGAACCGCT GCCAGAATGG TGGGACTTGT 
GTGGCCCAGG CCATGCTGGG GAAAGCCATG TGCCGATGTG CCTCAGGGTT TACAGGAGAG 
GACTGCCAGT ACTCGACATC TCATCCATGC TTTGTGTCTC GACCCTGCCT GAATGGCGGC 
ACATGCCATA TGCTCAGCCG GGATACCTAT GAGTGCACCT GTCAAGTCGG GTTTACAGGT 
AAGGAGTGCC AATGGACCGA TGCCTGCCTG TCTCATCCCT GTGCAAATGG AAGTACCTGT 
ACCACTGTGG CCAACCAGTT CTCCTGCAAA TGCCTCACAG GCTTCACAGG GCAGAAGTGT 
GAGACTGATG TCAATGAGTG TGACATTCCA GGACACTGCC AGCATGGTGG CACCTGCCTC 
AACCTGCCTG GTTCCTACCA GTGCCAGTGC CCTCAGGGCT TCACAGGCCA GTACTGTGAC 
AGCCTGTATG TGCCCTGTGC ACCCTCGCCT TGTGTCAATG GAGGCACCTG TCGGCAGACT 
GGTGACTTCA CTTTTGAGTG CAACTGCCTT CCAGGTTTTG AAGGGAGCAC CTGTGAGAGG 
AATATTGATG ACTGCCCTAA CCACAGGTGT CAGAATGGAG GGGTTTGTGT GGATGGGGTC 
AACACTTACA ACTGCCGCTG TCCCCCACAA TGGACAGGAC AGTTCTGCAC AGAGGATGTG 
GATGAATGCC TGCTGCAGCC CAATGCCTGT CAAAATGGGG GCACCTGTGC CAACCGCAAT 
GGAGGCTATG GCTGTGTATG TGTCAACGGC TGGAGTGGAG ATGACTGCAG TGAGAACATT 
GATGATTGTG CCTTCGCCTC CTGTACTCCA GGCTCCACCT GCATCGACCG TGTGGCCTCC 
TTCTCTTGCA TGTGCCCAGA GGGGAAGGCA GGTCTCCTGT GTCATCTGGA TGATGCATGC 
ATCAGCAATC CTTGCCACAA GGGGGCACTG TGTGACACCA ACCCCCTAAA TGGGCAATAT 
ATTTGCACCT GCCCACAAGG CTACAAAGGG GCTGACTGCA CAGAAGATGT GGATGAATGT 
TCCATGGCCA ATAGCAATCC TTGTGAGCAT GCAGGAAAAT GTGTGAACAC GGATGGCGCC 
TTCCACTGTG AGTGTCTGAA GGGTTATGCA GGACCTCGTT GTGAGATGGA CATCAATGAG 
TGCCATTCAG ACCCCTGCCA GAATGATGCT ACCTGTCTGG ATAAGATTGG AGGCTTCACA 
TGTCTGTGCA TGCCAGGTTT CAAAGGTGTG CATTGTGAAT TAGAAATAAA TGAATGTCAG 
AGCAACCCTT GTGTGAACAA TGGGCAGTGT GTGGATAAAG TCAATCGTTT CCAGTGCCTG 
TGTCCTCCTG GTTTCACTGG GCCAGTTTGC CAGATTGATA TTGATGACTG TTCCAGTACT 
CCGTGTCTGA ATGGGGCAAA GTGTATCGAT CACCCGAATG GCTATGAATG CCAGTGTGCC 
ACAGGTTTCA CTGGTGTGTT GTGTGAGGAG AACATTGACA ACTGTGACCC CGATCCTTGC 
CACCATGGTC AGTGTCAGGA TGGTATTGAT TCCTACACCT GCATCTGCAA TCCCGGGTAC 
ATGGGCGCCA TCTGCAGTGA CCAGATTGAT GAATGTTACA GCAGCCCTTG CCTGAACGAT 
GGTCGCTGCA TTGACCTGGT CAATGGCTAC CAGTGCAACT GCCAGCCGGG CACGTCAGGG 
GTTAATTGTG AAATTAATTT TGATGACTGT GCAAGTAACC CTTGTATCCA TGGAAACTGT 
ATGGATGGCA TTAATCGCTA CAGTTGTGTC TGCTCACCAG GATTCACAGG GCAGAGATGT 
AACATTGACA TTGATGAGTG TGCCTCCAAT CCCTGTCGCA AGGGTGCAAC ATGTATCAAC 
GGTGTGAATG GTTTCCGCTG TATATGCCCC GAGGGACCCC ATCACCCCAG CTGCTACTCA 
CAGGTGAACG AATGCCTGAG CAATCCCTGC ATCCATGGAA ACTGTACTGG AGGTCTCAGT 
GGATATAAGT GTCTCTGTGA TGCAGGCTGG GTTGGCATCA ACTGTGAAGT GGACAAAAAT 
GAATGCCTTT CGAATCCATG CCAGAATGGA GGAACTTGTG ACAATCTGGT GAATGGATAC 
AGGTGTACTT GCAAGAAGGG CTTTAAAGGC TATAACTGCC AGGTGAATAT TGATGAATGT 
GCCTCAAATC CATGCCTGAA CCAAGGAACC TGCTTTGATG ACATAAGTGG CTACACTTGC 
CACTGTGTGC TGCCATACAC AGGCAAGAAT TGTCAGACAG TATTGGCTCC CTGTTCCCCA 
AACCCTTGTG AGAATGCTGC TGTTTGCAAA GAGTCACCAA ATTTTGAGAG TTATACTTGC 
TTGTGTGCTC CTGGCTGGCA AGGTCAGCGG TGTACCATTG ACATTGACGA GTGTATCTCC 
AAGCCCTGCA TGAACCATGG TCTCTGCCAT AACACCCAGG GCAGCTACAT GTGTGAATGT 
CCACCAGGCT TCAGTGGTAT GGACTGTGAG GAGGACATTG ATGACTGCCT TGCCAATCCT 
TGCCAGAATG GAGGTTCCTG TGTGGATGGA GTGAATACTT TCTCCTGCCT CTGCCTTCCG 
GGTTTCACTG GGGATAAGTG CCAGACAGAC ATGAATGAGT GTCTGAGTGA ACCCTGTAAG 
AATGGAGGGA CCTGCTCTGA CTACGTCAAC AGTTACACTT GCAAGTGCCA GGCAGGATTT 
GATGGAGTCC ATTGTGAGAA CAACATCAAT GAGTGCACTG AGAGCTCCTG TTTCAATGGT 
GGCACATGTG TTGATGGGAT TAACTCCTTC TCTTGCTTGT GCCCTGTGGG TTTCACTGGA 
TCCTTCTGCC TCCATGAGAT CAATGAATGC AGCTCTCATC CATGCCTCAA TGAGGGAACG 
TGTGTTGATG GCCTGGGTAC CTACCGCTGC AGCTGCCCCC TGGGCTACAC TGGGAAAAAC 
TGTCAGACCC TGGTGAACCT CTGCAGTCGG TCTCCATGTA AAAACAAAGG TACTTGCGTT 
CAGAAAAAAG CAGAGTCCCA GTGCCTATGT CCATCTGGAT GGGCTGGTGC CTATTGTGAC 
GTGCCCAATG TCTCTTGTGA CATAGCAGCC TCCAGGAGAG GTGTGCTTGT TGAACACTTG 
TGCCAGCACT CAGGTGTCTG CATCAATGCT GGCAACACGC ATTACTGTCA GTGCCCCCTG 
GGCTATACTG GGAGCTACTG TGAGGAGCAA CTCGATGAGT GTGCGTCCAA CCCCTGCCAG 
CACGGGGCAA CATGCAGTGA CTTCATTGGT GGATACAGAT GTGAGTGTGT CCCAGGCTAT 
CAGGGTGTCA ACTGTGAGTA TGAAGTGGAT GAGTGCCAGA ATCAGCCCTG CCAGAATGGA 
GGCACCTGTA TTGACCTTGT GAACCATTTC AAGTGCTCTT GCCCACCAGG CACTCGGGGC 
CTACTCTGTG AAGAGAACAT TGATGACTGT GCCCGGGGTC CCCATTGCCT TAATGGTGGT 
CAGTGCATGG ATAGGATTGG AGGCTACAGT TGTCGCTGCT TGCCTGGCTT TGCTGGGGAG 
CGTTGTGAGG GAGACATCAA CGAGTGCCTC TCCAACCCCT GCAGCTCTGA GGGCAGCCTG 
GACTGTATAC AGCTCACCAA TGACTACCTG TGTGTTTGCC GTAGTGCCTT TACTGGCCGG 
CACTGTGAAA CCTTCGTCGA TGTGTGTCCC CAGATGCCCT GCCTGAATGG AGGGACTTGT 
GCTGTGGCCA GTAACATGCC TGATGGTTTC ATTTGCCGTT GTCCCCCGGG ATTTTCTGGG 
GCAAGGTGCC AGAGCAGCTG TGGACAAGTG AAATGTAGGA AAGGGGAGCA GTGTGTGCAC 
ACCGCCTCTG GACCCCGCTG CTTCTGCCCC AGTCCCCGGG ACTGCGAGTC AGGCTGTGCC 
AGTAGCCCCT GCCAGCACGG GGGCAGCTGC CACCCTCAGC GCCAGCCTCC TTATTACTCC 
TGCCAGTGTG CCCCACCATT CTCGGGTAGC CGCTGTGAAC TCTACACGGC ACCCCCCAGC 
ACCCCTCCTG CCACCTGTCT GAGCCAGTAT TGTGCCGACA AAGCTCGGGA TGGCGTCTGT 
GATGAGGCCT GCAACAGCCA TGCCTGCCAG TGGGATGGGG GTGACTGTTC TCTCACCATG 
GAGAACCCCT GGGCCAACTG CTCCTCCCCA CTTCCCTGCT GGGATTATAT CAACAACCAG 
TGTGATGAGC TGTGCAACAC GGTCGAGTGC CTGTTTGACA ACTTTGAATG CCAGGGGAAC 
AGCAAGACAT GCAAGTATGA CAAATACTGT GCAGACCACT TCAAAGACAA CCACTGTGAC 
CAGGGGTGCA ACAGTGAGGA GTGTGGTTGG GATGGGCTGG ACTGTGCTGC TGACCAACCT 
GAGAACCTGG CAGAAGGTAC CCTGGTTATT GTGGTATTGA TGCCACCTGA ACAACTGCTC 
CAGGATGCTC GCAGCTTCTT GCGGGCACTG GGTACCCTGC TGCACACCAA CCTGCGCATT 
AAGCGGGACT CCCAGGGGGA ACTCATGGTG TACCCCTATT ATGGTGAGAA GTCAGCTGCT 
ATGAAGAAAC AGAGGATGAC ACGCAGGTCC CTTCCTGGTG AACAAGAACA GGAGGTGGCT 
GGCTCTAAAG TCTTTCTGGA AATTGACAAC CGCCAGTGTG TTCAAGACTC AGACCACTGC 
TTCAAGAACA CGGATGCAGC AGCAGCTCTC CTGGCCTCTC ACGCCATACA GGGGACCCTG 
TCATACCCTC TTGTGTCTGT CGTCAGTGAA TCCCTGACTC CAGAACGCAC TCAGCTCCTC 
TATCTCCTTG CTGTTGCTGT TGTCATCATT CTGTTTATTA TTCTGCTGGG GGTAATCATG 
GCAAAACGAA AGCGTAAGCA TGGCTCTCTC TGGCTGCCTG AAGGTTTCAC TCTTCGCCGA 
GATGCAAGCA ATCACAAGCG TCGTGAGCCA GTGGGACAGG ATGCTGTGGG GCTAAAAAAT 
CTCTCAGTGC AAGTCTCAGA AGCTAACCTA ATTGGTTCTG GAACAAGTGA ACACTGGGTC 
GATGATGAAG GGCCCCAGCC AAAGAAAGTA AAGGCTGAAG ATGAGGCCTT ACTCTCAGAA 
GAAGATGACC CCATTGATCG ACGGCCATGG ACACAGCAGC ACCTTGAAGC CGCAGACATC 
CGTAGGACAC CATCGCTGGC TCTCACCCCT CCTCAGGCAG AGCAGGAGGT GGATGTGTTA 
GATGTGAATG TCCGTGGCCC AGATGGCTGC ACCCCATTGA TGTTGGCTTC TCTCCGAGGA 
GGCAGCTCAG ATTTGAGTGA TGAAGATGAA GATGCAGAGG ACTCTTCTGC TAACATCATC 
ACAGACTTGG TCTACCAGGG TGCCAGCCTC CAGGCCCAGA CAGACCGGAC TGGTGAGATG 
GCCCTGCACC TTGCAGCCCG CTACTCACGG GCTGATGCTG CCAAGCGTCT CCTGGATGCA 
GGTGCAGATG CCAATGCCCA GGACAACATG GGCCGCTGCC CACTCCATGC TGCAGTGGCA 
GCTGATGCCC AAGGTGTCTT CCAGATTCTG ATTCGCAACC GAGTAACTGA TCTAGATGCC 
AGGATGAATG ATGGTACTAC ACCCCTGATC CTGGCTGCCC GCCTGGCTGT GGAGGGAATG 
GTGGCAGAAC TGATCAACTG CCAAGCGGAT GTGAATGCAG TGGATGACCA TGGAAAATCT 
GCTCTTCACT GGGCAGCTGC TGTCAATAAT GTGGAGGCAA CTCTTTTGTT GTTGAAAAAT 
GGAGCCAACC GAGACATGCA GGACAACAAG GAAGAGACAC CTCTGTTTCT TGCTGCCCGG 
GAGGGGAGCT ATGAAGCAGC CAAGATCCTG TTAGACCATT TTGCCAATCG AGACATCACA 
GACCATATGG ATCGTCTTCC CCGGGATGTG GCTCGGGATC GCATGCACCA TGACATTGTG 
CGCCTTCTGG ATGAATACAA TGTGACCCCA AGCCCTCCAG GCACCGTGTT GACTTCTGCT 
CTCTCACCTG TCATCTGTGG GCCCAACAGA TCTTTCCTCA GCCTGAAGCA CACCCCAATG 
GGCAAGAAGT CTAGACGGCC CAGTGCCAAG AGTACCATGC CTACTAGCCT CCCTAACCTT 
GCCAAGGAGG CAAAGGATGC CAAGGGTAGT AGGAGGAAGA AGTCTCTGAG TGAGAAGGTC 
CAACTGTCTG AGAGTTCAGT AACTTTATCC CCTGTTGATT CCCTAGAATC TCCTCACACG 
TATGTTTCCG ACACCACATC CTCTCCAATG ATTACATCCC CTGGGATCTT ACAGGCCTCA 
CCCAACCCTA TGTTGGCCAC TGCCGCCCCT CCTGCCCCAG TCCATGCCCA GCATGCACTA 
TCTTTTTCTA ACCTTCATGA AATGCAGCCT TTGGCACATG GGGCCAGCAC TGTGCTTCCC 
TCAGTGAGCC AGTTGCTATC CCACCACCAC ATTGTGTCTC CAGGCAGTGG CAGTGCTGGA 
AGCTTGAGTA GGCTCCATCC AGTCCCAGTC CCAGCAGATT GGATGAACCG CATGGAGGTG 
AATGAGACCC AGTACAATGA GATGTTTGGT ATGGTCCTGG CTCCAGCTGA GGGCACCCAT 
CCTGGCATAG CTCCCCAGAG CAGGCCACCT GAAGGGAAGC ACATAACCAC CCCTCGGGAG 
CCCTTGCCCC CCATTGTGAC TTTCCAGCTC ATCCCTAAAG GCAGTATTGC CCAACCAGCG 
GGGGCTCCCC AGCCTCAGTC CACCTGCCCT CCAGCTGTTG CGGGCCCCCT GCCCACCATG 
TACCAGATTC CAGAAATGGC CCGTTTGCCC AGTGTGGCTT TCCCCACTGC CATGATGCCC 
CAGCAGGACG GGCAGGTAGC TCAGACCATT CTCCCAGCCT ATCATCCTTT CCCAGCCTCT 
GTGGGCAAGT ACCCCACACC CCCTTCACAG CACAGTTATG CTTCCTCAAA TGCTGCTGAG 
CGAACACCCA GTCACAGTGG TCACCTCCAG GGTGAGCATC CCTACCTGAC ACCATCCCCA 
GAGTCTCCTG ACCAGTGGTC AAGTTCATCA CCCCACTCTG CTTCTGACTG GTCAGATGTG 
ACCACCAGCC CTACCCCTGG GGGTGCTGGA GGAGGTCAGC GGGGACCTGG GACACACATG 
TCTGAGCCAC CACACAACAA CATGCAGGTT TATGCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NOTCH2 ( XM_016924542.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016780031.1 Pan troglodytes notch 2 (NOTCH2), transcript variant X3, mRNA.

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CloneID	OPc59962
Clone ID Related Accession (Same CDS sequence)	XM_016924542.1 , XM_016924549.1
Accession Version	XM_016924542.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7167bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	neurogenic locus notch homolog protein 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006468.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGGGGA AAGCCATGTG CCGATGTGCC TCAGGGTTTA CAGGAGAGGA CTGCCAGTAC 
TCGACATCTC ATCCATGCTT TGTGTCTCGA CCCTGCCTGA ATGGCGGCAC ATGCCATATG 
CTCAGCCGGG ATACCTATGA GTGCACCTGT CAAGTCGGGT TTACAGGTAA GGAGTGCCAA 
TGGACCGATG CCTGCCTGTC TCATCCCTGT GCAAATGGAA GTACCTGTAC CACTGTGGCC 
AACCAGTTCT CCTGCAAATG CCTCACAGGC TTCACAGGGC AGAAGTGTGA GACTGATGTC 
AATGAGTGTG ACATTCCAGG ACACTGCCAG CATGGTGGCA CCTGCCTCAA CCTGCCTGGT 
TCCTACCAGT GCCAGTGCCC TCAGGGCTTC ACAGGCCAGT ACTGTGACAG CCTGTATGTG 
CCCTGTGCAC CCTCGCCTTG TGTCAATGGA GGCACCTGTC GGCAGACTGG TGACTTCACT 
TTTGAGTGCA ACTGCCTTCC AGGTTTTGAA GGGAGCACCT GTGAGAGGAA TATTGATGAC 
TGCCCTAACC ACAGGTGTCA GAATGGAGGG GTTTGTGTGG ATGGGGTCAA CACTTACAAC 
TGCCGCTGTC CCCCACAATG GACAGGACAG TTCTGCACAG AGGATGTGGA TGAATGCCTG 
CTGCAGCCCA ATGCCTGTCA AAATGGGGGC ACCTGTGCCA ACCGCAATGG AGGCTATGGC 
TGTGTATGTG TCAACGGCTG GAGTGGAGAT GACTGCAGTG AGAACATTGA TGATTGTGCC 
TTCGCCTCCT GTACTCCAGG CTCCACCTGC ATCGACCGTG TGGCCTCCTT CTCTTGCATG 
TGCCCAGAGG GGAAGGCAGG TCTCCTGTGT CATCTGGATG ATGCATGCAT CAGCAATCCT 
TGCCACAAGG GGGCACTGTG TGACACCAAC CCCCTAAATG GGCAATATAT TTGCACCTGC 
CCACAAGGCT ACAAAGGGGC TGACTGCACA GAAGATGTGG ATGAATGTTC CATGGCCAAT 
AGCAATCCTT GTGAGCATGC AGGAAAATGT GTGAACACGG ATGGCGCCTT CCACTGTGAG 
TGTCTGAAGG GTTATGCAGG ACCTCGTTGT GAGATGGACA TCAATGAGTG CCATTCAGAC 
CCCTGCCAGA ATGATGCTAC CTGTCTGGAT AAGATTGGAG GCTTCACATG TCTGTGCATG 
CCAGGTTTCA AAGGTGTGCA TTGTGAATTA GAAATAAATG AATGTCAGAG CAACCCTTGT 
GTGAACAATG GGCAGTGTGT GGATAAAGTC AATCGTTTCC AGTGCCTGTG TCCTCCTGGT 
TTCACTGGGC CAGTTTGCCA GATTGATATT GATGACTGTT CCAGTACTCC GTGTCTGAAT 
GGGGCAAAGT GTATCGATCA CCCGAATGGC TATGAATGCC AGTGTGCCAC AGGTTTCACT 
GGTGTGTTGT GTGAGGAGAA CATTGACAAC TGTGACCCCG ATCCTTGCCA CCATGGTCAG 
TGTCAGGATG GTATTGATTC CTACACCTGC ATCTGCAATC CCGGGTACAT GGGCGCCATC 
TGCAGTGACC AGATTGATGA ATGTTACAGC AGCCCTTGCC TGAACGATGG TCGCTGCATT 
GACCTGGTCA ATGGCTACCA GTGCAACTGC CAGCCGGGCA CGTCAGGGGT TAATTGTGAA 
ATTAATTTTG ATGACTGTGC AAGTAACCCT TGTATCCATG GAAACTGTAT GGATGGCATT 
AATCGCTACA GTTGTGTCTG CTCACCAGGA TTCACAGGGC AGAGATGTAA CATTGACATT 
GATGAGTGTG CCTCCAATCC CTGTCGCAAG GGTGCAACAT GTATCAACGG TGTGAATGGT 
TTCCGCTGTA TATGCCCCGA GGGACCCCAT CACCCCAGCT GCTACTCACA GGTGAACGAA 
TGCCTGAGCA ATCCCTGCAT CCATGGAAAC TGTACTGGAG GTCTCAGTGG ATATAAGTGT 
CTCTGTGATG CAGGCTGGGT TGGCATCAAC TGTGAAGTGG ACAAAAATGA ATGCCTTTCG 
AATCCATGCC AGAATGGAGG AACTTGTGAC AATCTGGTGA ATGGATACAG GTGTACTTGC 
AAGAAGGGCT TTAAAGGCTA TAACTGCCAG GTGAATATTG ATGAATGTGC CTCAAATCCA 
TGCCTGAACC AAGGAACCTG CTTTGATGAC ATAAGTGGCT ACACTTGCCA CTGTGTGCTG 
CCATACACAG GCAAGAATTG TCAGACAGTA TTGGCTCCCT GTTCCCCAAA CCCTTGTGAG 
AATGCTGCTG TTTGCAAAGA GTCACCAAAT TTTGAGAGTT ATACTTGCTT GTGTGCTCCT 
GGCTGGCAAG GTCAGCGGTG TACCATTGAC ATTGACGAGT GTATCTCCAA GCCCTGCATG 
AACCATGGTC TCTGCCATAA CACCCAGGGC AGCTACATGT GTGAATGTCC ACCAGGCTTC 
AGTGGTATGG ACTGTGAGGA GGACATTGAT GACTGCCTTG CCAATCCTTG CCAGAATGGA 
GGTTCCTGTG TGGATGGAGT GAATACTTTC TCCTGCCTCT GCCTTCCGGG TTTCACTGGG 
GATAAGTGCC AGACAGACAT GAATGAGTGT CTGAGTGAAC CCTGTAAGAA TGGAGGGACC 
TGCTCTGACT ACGTCAACAG TTACACTTGC AAGTGCCAGG CAGGATTTGA TGGAGTCCAT 
TGTGAGAACA ACATCAATGA GTGCACTGAG AGCTCCTGTT TCAATGGTGG CACATGTGTT 
GATGGGATTA ACTCCTTCTC TTGCTTGTGC CCTGTGGGTT TCACTGGATC CTTCTGCCTC 
CATGAGATCA ATGAATGCAG CTCTCATCCA TGCCTCAATG AGGGAACGTG TGTTGATGGC 
CTGGGTACCT ACCGCTGCAG CTGCCCCCTG GGCTACACTG GGAAAAACTG TCAGACCCTG 
GTGAACCTCT GCAGTCGGTC TCCATGTAAA AACAAAGGTA CTTGCGTTCA GAAAAAAGCA 
GAGTCCCAGT GCCTATGTCC ATCTGGATGG GCTGGTGCCT ATTGTGACGT GCCCAATGTC 
TCTTGTGACA TAGCAGCCTC CAGGAGAGGT GTGCTTGTTG AACACTTGTG CCAGCACTCA 
GGTGTCTGCA TCAATGCTGG CAACACGCAT TACTGTCAGT GCCCCCTGGG CTATACTGGG 
AGCTACTGTG AGGAGCAACT CGATGAGTGT GCGTCCAACC CCTGCCAGCA CGGGGCAACA 
TGCAGTGACT TCATTGGTGG ATACAGATGT GAGTGTGTCC CAGGCTATCA GGGTGTCAAC 
TGTGAGTATG AAGTGGATGA GTGCCAGAAT CAGCCCTGCC AGAATGGAGG CACCTGTATT 
GACCTTGTGA ACCATTTCAA GTGCTCTTGC CCACCAGGCA CTCGGGGCCT ACTCTGTGAA 
GAGAACATTG ATGACTGTGC CCGGGGTCCC CATTGCCTTA ATGGTGGTCA GTGCATGGAT 
AGGATTGGAG GCTACAGTTG TCGCTGCTTG CCTGGCTTTG CTGGGGAGCG TTGTGAGGGA 
GACATCAACG AGTGCCTCTC CAACCCCTGC AGCTCTGAGG GCAGCCTGGA CTGTATACAG 
CTCACCAATG ACTACCTGTG TGTTTGCCGT AGTGCCTTTA CTGGCCGGCA CTGTGAAACC 
TTCGTCGATG TGTGTCCCCA GATGCCCTGC CTGAATGGAG GGACTTGTGC TGTGGCCAGT 
AACATGCCTG ATGGTTTCAT TTGCCGTTGT CCCCCGGGAT TTTCTGGGGC AAGGTGCCAG 
AGCAGCTGTG GACAAGTGAA ATGTAGGAAA GGGGAGCAGT GTGTGCACAC CGCCTCTGGA 
CCCCGCTGCT TCTGCCCCAG TCCCCGGGAC TGCGAGTCAG GCTGTGCCAG TAGCCCCTGC 
CAGCACGGGG GCAGCTGCCA CCCTCAGCGC CAGCCTCCTT ATTACTCCTG CCAGTGTGCC 
CCACCATTCT CGGGTAGCCG CTGTGAACTC TACACGGCAC CCCCCAGCAC CCCTCCTGCC 
ACCTGTCTGA GCCAGTATTG TGCCGACAAA GCTCGGGATG GCGTCTGTGA TGAGGCCTGC 
AACAGCCATG CCTGCCAGTG GGATGGGGGT GACTGTTCTC TCACCATGGA GAACCCCTGG 
GCCAACTGCT CCTCCCCACT TCCCTGCTGG GATTATATCA ACAACCAGTG TGATGAGCTG 
TGCAACACGG TCGAGTGCCT GTTTGACAAC TTTGAATGCC AGGGGAACAG CAAGACATGC 
AAGTATGACA AATACTGTGC AGACCACTTC AAAGACAACC ACTGTGACCA GGGGTGCAAC 
AGTGAGGAGT GTGGTTGGGA TGGGCTGGAC TGTGCTGCTG ACCAACCTGA GAACCTGGCA 
GAAGGTACCC TGGTTATTGT GGTATTGATG CCACCTGAAC AACTGCTCCA GGATGCTCGC 
AGCTTCTTGC GGGCACTGGG TACCCTGCTG CACACCAACC TGCGCATTAA GCGGGACTCC 
CAGGGGGAAC TCATGGTGTA CCCCTATTAT GGTGAGAAGT CAGCTGCTAT GAAGAAACAG 
AGGATGACAC GCAGGTCCCT TCCTGGTGAA CAAGAACAGG AGGTGGCTGG CTCTAAAGTC 
TTTCTGGAAA TTGACAACCG CCAGTGTGTT CAAGACTCAG ACCACTGCTT CAAGAACACG 
GATGCAGCAG CAGCTCTCCT GGCCTCTCAC GCCATACAGG GGACCCTGTC ATACCCTCTT 
GTGTCTGTCG TCAGTGAATC CCTGACTCCA GAACGCACTC AGCTCCTCTA TCTCCTTGCT 
GTTGCTGTTG TCATCATTCT GTTTATTATT CTGCTGGGGG TAATCATGGC AAAACGAAAG 
CGTAAGCATG GCTCTCTCTG GCTGCCTGAA GGTTTCACTC TTCGCCGAGA TGCAAGCAAT 
CACAAGCGTC GTGAGCCAGT GGGACAGGAT GCTGTGGGGC TAAAAAATCT CTCAGTGCAA 
GTCTCAGAAG CTAACCTAAT TGGTTCTGGA ACAAGTGAAC ACTGGGTCGA TGATGAAGGG 
CCCCAGCCAA AGAAAGTAAA GGCTGAAGAT GAGGCCTTAC TCTCAGAAGA AGATGACCCC 
ATTGATCGAC GGCCATGGAC ACAGCAGCAC CTTGAAGCCG CAGACATCCG TAGGACACCA 
TCGCTGGCTC TCACCCCTCC TCAGGCAGAG CAGGAGGTGG ATGTGTTAGA TGTGAATGTC 
CGTGGCCCAG ATGGCTGCAC CCCATTGATG TTGGCTTCTC TCCGAGGAGG CAGCTCAGAT 
TTGAGTGATG AAGATGAAGA TGCAGAGGAC TCTTCTGCTA ACATCATCAC AGACTTGGTC 
TACCAGGGTG CCAGCCTCCA GGCCCAGACA GACCGGACTG GTGAGATGGC CCTGCACCTT 
GCAGCCCGCT ACTCACGGGC TGATGCTGCC AAGCGTCTCC TGGATGCAGG TGCAGATGCC 
AATGCCCAGG ACAACATGGG CCGCTGCCCA CTCCATGCTG CAGTGGCAGC TGATGCCCAA 
GGTGTCTTCC AGATTCTGAT TCGCAACCGA GTAACTGATC TAGATGCCAG GATGAATGAT 
GGTACTACAC CCCTGATCCT GGCTGCCCGC CTGGCTGTGG AGGGAATGGT GGCAGAACTG 
ATCAACTGCC AAGCGGATGT GAATGCAGTG GATGACCATG GAAAATCTGC TCTTCACTGG 
GCAGCTGCTG TCAATAATGT GGAGGCAACT CTTTTGTTGT TGAAAAATGG AGCCAACCGA 
GACATGCAGG ACAACAAGGA AGAGACACCT CTGTTTCTTG CTGCCCGGGA GGGGAGCTAT 
GAAGCAGCCA AGATCCTGTT AGACCATTTT GCCAATCGAG ACATCACAGA CCATATGGAT 
CGTCTTCCCC GGGATGTGGC TCGGGATCGC ATGCACCATG ACATTGTGCG CCTTCTGGAT 
GAATACAATG TGACCCCAAG CCCTCCAGGC ACCGTGTTGA CTTCTGCTCT CTCACCTGTC 
ATCTGTGGGC CCAACAGATC TTTCCTCAGC CTGAAGCACA CCCCAATGGG CAAGAAGTCT 
AGACGGCCCA GTGCCAAGAG TACCATGCCT ACTAGCCTCC CTAACCTTGC CAAGGAGGCA 
AAGGATGCCA AGGGTAGTAG GAGGAAGAAG TCTCTGAGTG AGAAGGTCCA ACTGTCTGAG 
AGTTCAGTAA CTTTATCCCC TGTTGATTCC CTAGAATCTC CTCACACGTA TGTTTCCGAC 
ACCACATCCT CTCCAATGAT TACATCCCCT GGGATCTTAC AGGCCTCACC CAACCCTATG 
TTGGCCACTG CCGCCCCTCC TGCCCCAGTC CATGCCCAGC ATGCACTATC TTTTTCTAAC 
CTTCATGAAA TGCAGCCTTT GGCACATGGG GCCAGCACTG TGCTTCCCTC AGTGAGCCAG 
TTGCTATCCC ACCACCACAT TGTGTCTCCA GGCAGTGGCA GTGCTGGAAG CTTGAGTAGG 
CTCCATCCAG TCCCAGTCCC AGCAGATTGG ATGAACCGCA TGGAGGTGAA TGAGACCCAG 
TACAATGAGA TGTTTGGTAT GGTCCTGGCT CCAGCTGAGG GCACCCATCC TGGCATAGCT 
CCCCAGAGCA GGCCACCTGA AGGGAAGCAC ATAACCACCC CTCGGGAGCC CTTGCCCCCC 
ATTGTGACTT TCCAGCTCAT CCCTAAAGGC AGTATTGCCC AACCAGCGGG GGCTCCCCAG 
CCTCAGTCCA CCTGCCCTCC AGCTGTTGCG GGCCCCCTGC CCACCATGTA CCAGATTCCA 
GAAATGGCCC GTTTGCCCAG TGTGGCTTTC CCCACTGCCA TGATGCCCCA GCAGGACGGG 
CAGGTAGCTC AGACCATTCT CCCAGCCTAT CATCCTTTCC CAGCCTCTGT GGGCAAGTAC 
CCCACACCCC CTTCACAGCA CAGTTATGCT TCCTCAAATG CTGCTGAGCG AACACCCAGT 
CACAGTGGTC ACCTCCAGGG TGAGCATCCC TACCTGACAC CATCCCCAGA GTCTCCTGAC 
CAGTGGTCAA GTTCATCACC CCACTCTGCT TCTGACTGGT CAGATGTGAC CACCAGCCCT 
ACCCCTGGGG GTGCTGGAGG AGGTCAGCGG GGACCTGGGA CACACATGTC TGAGCCACCA 
CACAACAACA TGCAGGTTTA TGCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016780031.1
CDS	386..7552
Translation	MLGKAMCRCASGFTGEDCQYSTSHPCFVSRPCLNGGTCHMLSRDTYECTCQVGFTGKECQWTDACLSHPCANGSTCTTVANQFSCKCLTGFTGQKCETDVNECDIPGHCQHGGTCLNLPGSYQCQCPQGFTGQYCDSLYVPCAPSPCVNGGTCRQTGDFTFECNCLPGFEGSTCERNIDDCPNHRCQNGGVCVDGVNTYNCRCPPQWTGQFCTEDVDECLLQPNACQNGGTCANRNGGYGCVCVNGWSGDDCSENIDDCAFASCTPGSTCIDRVASFSCMCPEGKAGLLCHLDDACISNPCHKGALCDTNPLNGQYICTCPQGYKGADCTEDVDECSMANSNPCEHAGKCVNTDGAFHCECLKGYAGPRCEMDINECHSDPCQNDATCLDKIGGFTCLCMPGFKGVHCELEINECQSNPCVNNGQCVDKVNRFQCLCPPGFTGPVCQIDIDDCSSTPCLNGAKCIDHPNGYECQCATGFTGVLCEENIDNCDPDPCHHGQCQDGIDSYTCICNPGYMGAICSDQIDECYSSPCLNDGRCIDLVNGYQCNCQPGTSGVNCEINFDDCASNPCIHGNCMDGINRYSCVCSPGFTGQRCNIDIDECASNPCRKGATCINGVNGFRCICPEGPHHPSCYSQVNECLSNPCIHGNCTGGLSGYKCLCDAGWVGINCEVDKNECLSNPCQNGGTCDNLVNGYRCTCKKGFKGYNCQVNIDECASNPCLNQGTCFDDISGYTCHCVLPYTGKNCQTVLAPCSPNPCENAAVCKESPNFESYTCLCAPGWQGQRCTIDIDECISKPCMNHGLCHNTQGSYMCECPPGFSGMDCEEDIDDCLANPCQNGGSCVDGVNTFSCLCLPGFTGDKCQTDMNECLSEPCKNGGTCSDYVNSYTCKCQAGFDGVHCENNINECTESSCFNGGTCVDGINSFSCLCPVGFTGSFCLHEINECSSHPCLNEGTCVDGLGTYRCSCPLGYTGKNCQTLVNLCSRSPCKNKGTCVQKKAESQCLCPSGWAGAYCDVPNVSCDIAASRRGVLVEHLCQHSGVCINAGNTHYCQCPLGYTGSYCEEQLDECASNPCQHGATCSDFIGGYRCECVPGYQGVNCEYEVDECQNQPCQNGGTCIDLVNHFKCSCPPGTRGLLCEENIDDCARGPHCLNGGQCMDRIGGYSCRCLPGFAGERCEGDINECLSNPCSSEGSLDCIQLTNDYLCVCRSAFTGRHCETFVDVCPQMPCLNGGTCAVASNMPDGFICRCPPGFSGARCQSSCGQVKCRKGEQCVHTASGPRCFCPSPRDCESGCASSPCQHGGSCHPQRQPPYYSCQCAPPFSGSRCELYTAPPSTPPATCLSQYCADKARDGVCDEACNSHACQWDGGDCSLTMENPWANCSSPLPCWDYINNQCDELCNTVECLFDNFECQGNSKTCKYDKYCADHFKDNHCDQGCNSEECGWDGLDCAADQPENLAEGTLVIVVLMPPEQLLQDARSFLRALGTLLHTNLRIKRDSQGELMVYPYYGEKSAAMKKQRMTRRSLPGEQEQEVAGSKVFLEIDNRQCVQDSDHCFKNTDAAAALLASHAIQGTLSYPLVSVVSESLTPERTQLLYLLAVAVVIILFIILLGVIMAKRKRKHGSLWLPEGFTLRRDASNHKRREPVGQDAVGLKNLSVQVSEANLIGSGTSEHWVDDEGPQPKKVKAEDEALLSEEDDPIDRRPWTQQHLEAADIRRTPSLALTPPQAEQEVDVLDVNVRGPDGCTPLMLASLRGGSSDLSDEDEDAEDSSANIITDLVYQGASLQAQTDRTGEMALHLAARYSRADAAKRLLDAGADANAQDNMGRCPLHAAVAADAQGVFQILIRNRVTDLDARMNDGTTPLILAARLAVEGMVAELINCQADVNAVDDHGKSALHWAAAVNNVEATLLLLKNGANRDMQDNKEETPLFLAAREGSYEAAKILLDHFANRDITDHMDRLPRDVARDRMHHDIVRLLDEYNVTPSPPGTVLTSALSPVICGPNRSFLSLKHTPMGKKSRRPSAKSTMPTSLPNLAKEAKDAKGSRRKKSLSEKVQLSESSVTLSPVDSLESPHTYVSDTTSSPMITSPGILQASPNPMLATAAPPAPVHAQHALSFSNLHEMQPLAHGASTVLPSVSQLLSHHHIVSPGSGSAGSLSRLHPVPVPADWMNRMEVNETQYNEMFGMVLAPAEGTHPGIAPQSRPPEGKHITTPREPLPPIVTFQLIPKGSIAQPAGAPQPQSTCPPAVAGPLPTMYQIPEMARLPSVAFPTAMMPQQDGQVAQTILPAYHPFPASVGKYPTPPSQHSYASSNAAERTPSHSGHLQGEHPYLTPSPESPDQWSSSSPHSASDWSDVTTSPTPGGAGGGQRGPGTHMSEPPHNNMQVYA

Target ORF information:

RefSeq Version	XM_016924542.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes notch 2 (NOTCH2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016924542.1

ORF Insert Sequence:

ATGCTGGGGA AAGCCATGTG CCGATGTGCC TCAGGGTTTA CAGGAGAGGA CTGCCAGTAC 
TCGACATCTC ATCCATGCTT TGTGTCTCGA CCCTGCCTGA ATGGCGGCAC ATGCCATATG 
CTCAGCCGGG ATACCTATGA GTGCACCTGT CAAGTCGGGT TTACAGGTAA GGAGTGCCAA 
TGGACCGATG CCTGCCTGTC TCATCCCTGT GCAAATGGAA GTACCTGTAC CACTGTGGCC 
AACCAGTTCT CCTGCAAATG CCTCACAGGC TTCACAGGGC AGAAGTGTGA GACTGATGTC 
AATGAGTGTG ACATTCCAGG ACACTGCCAG CATGGTGGCA CCTGCCTCAA CCTGCCTGGT 
TCCTACCAGT GCCAGTGCCC TCAGGGCTTC ACAGGCCAGT ACTGTGACAG CCTGTATGTG 
CCCTGTGCAC CCTCGCCTTG TGTCAATGGA GGCACCTGTC GGCAGACTGG TGACTTCACT 
TTTGAGTGCA ACTGCCTTCC AGGTTTTGAA GGGAGCACCT GTGAGAGGAA TATTGATGAC 
TGCCCTAACC ACAGGTGTCA GAATGGAGGG GTTTGTGTGG ATGGGGTCAA CACTTACAAC 
TGCCGCTGTC CCCCACAATG GACAGGACAG TTCTGCACAG AGGATGTGGA TGAATGCCTG 
CTGCAGCCCA ATGCCTGTCA AAATGGGGGC ACCTGTGCCA ACCGCAATGG AGGCTATGGC 
TGTGTATGTG TCAACGGCTG GAGTGGAGAT GACTGCAGTG AGAACATTGA TGATTGTGCC 
TTCGCCTCCT GTACTCCAGG CTCCACCTGC ATCGACCGTG TGGCCTCCTT CTCTTGCATG 
TGCCCAGAGG GGAAGGCAGG TCTCCTGTGT CATCTGGATG ATGCATGCAT CAGCAATCCT 
TGCCACAAGG GGGCACTGTG TGACACCAAC CCCCTAAATG GGCAATATAT TTGCACCTGC 
CCACAAGGCT ACAAAGGGGC TGACTGCACA GAAGATGTGG ATGAATGTTC CATGGCCAAT 
AGCAATCCTT GTGAGCATGC AGGAAAATGT GTGAACACGG ATGGCGCCTT CCACTGTGAG 
TGTCTGAAGG GTTATGCAGG ACCTCGTTGT GAGATGGACA TCAATGAGTG CCATTCAGAC 
CCCTGCCAGA ATGATGCTAC CTGTCTGGAT AAGATTGGAG GCTTCACATG TCTGTGCATG 
CCAGGTTTCA AAGGTGTGCA TTGTGAATTA GAAATAAATG AATGTCAGAG CAACCCTTGT 
GTGAACAATG GGCAGTGTGT GGATAAAGTC AATCGTTTCC AGTGCCTGTG TCCTCCTGGT 
TTCACTGGGC CAGTTTGCCA GATTGATATT GATGACTGTT CCAGTACTCC GTGTCTGAAT 
GGGGCAAAGT GTATCGATCA CCCGAATGGC TATGAATGCC AGTGTGCCAC AGGTTTCACT 
GGTGTGTTGT GTGAGGAGAA CATTGACAAC TGTGACCCCG ATCCTTGCCA CCATGGTCAG 
TGTCAGGATG GTATTGATTC CTACACCTGC ATCTGCAATC CCGGGTACAT GGGCGCCATC 
TGCAGTGACC AGATTGATGA ATGTTACAGC AGCCCTTGCC TGAACGATGG TCGCTGCATT 
GACCTGGTCA ATGGCTACCA GTGCAACTGC CAGCCGGGCA CGTCAGGGGT TAATTGTGAA 
ATTAATTTTG ATGACTGTGC AAGTAACCCT TGTATCCATG GAAACTGTAT GGATGGCATT 
AATCGCTACA GTTGTGTCTG CTCACCAGGA TTCACAGGGC AGAGATGTAA CATTGACATT 
GATGAGTGTG CCTCCAATCC CTGTCGCAAG GGTGCAACAT GTATCAACGG TGTGAATGGT 
TTCCGCTGTA TATGCCCCGA GGGACCCCAT CACCCCAGCT GCTACTCACA GGTGAACGAA 
TGCCTGAGCA ATCCCTGCAT CCATGGAAAC TGTACTGGAG GTCTCAGTGG ATATAAGTGT 
CTCTGTGATG CAGGCTGGGT TGGCATCAAC TGTGAAGTGG ACAAAAATGA ATGCCTTTCG 
AATCCATGCC AGAATGGAGG AACTTGTGAC AATCTGGTGA ATGGATACAG GTGTACTTGC 
AAGAAGGGCT TTAAAGGCTA TAACTGCCAG GTGAATATTG ATGAATGTGC CTCAAATCCA 
TGCCTGAACC AAGGAACCTG CTTTGATGAC ATAAGTGGCT ACACTTGCCA CTGTGTGCTG 
CCATACACAG GCAAGAATTG TCAGACAGTA TTGGCTCCCT GTTCCCCAAA CCCTTGTGAG 
AATGCTGCTG TTTGCAAAGA GTCACCAAAT TTTGAGAGTT ATACTTGCTT GTGTGCTCCT 
GGCTGGCAAG GTCAGCGGTG TACCATTGAC ATTGACGAGT GTATCTCCAA GCCCTGCATG 
AACCATGGTC TCTGCCATAA CACCCAGGGC AGCTACATGT GTGAATGTCC ACCAGGCTTC 
AGTGGTATGG ACTGTGAGGA GGACATTGAT GACTGCCTTG CCAATCCTTG CCAGAATGGA 
GGTTCCTGTG TGGATGGAGT GAATACTTTC TCCTGCCTCT GCCTTCCGGG TTTCACTGGG 
GATAAGTGCC AGACAGACAT GAATGAGTGT CTGAGTGAAC CCTGTAAGAA TGGAGGGACC 
TGCTCTGACT ACGTCAACAG TTACACTTGC AAGTGCCAGG CAGGATTTGA TGGAGTCCAT 
TGTGAGAACA ACATCAATGA GTGCACTGAG AGCTCCTGTT TCAATGGTGG CACATGTGTT 
GATGGGATTA ACTCCTTCTC TTGCTTGTGC CCTGTGGGTT TCACTGGATC CTTCTGCCTC 
CATGAGATCA ATGAATGCAG CTCTCATCCA TGCCTCAATG AGGGAACGTG TGTTGATGGC 
CTGGGTACCT ACCGCTGCAG CTGCCCCCTG GGCTACACTG GGAAAAACTG TCAGACCCTG 
GTGAACCTCT GCAGTCGGTC TCCATGTAAA AACAAAGGTA CTTGCGTTCA GAAAAAAGCA 
GAGTCCCAGT GCCTATGTCC ATCTGGATGG GCTGGTGCCT ATTGTGACGT GCCCAATGTC 
TCTTGTGACA TAGCAGCCTC CAGGAGAGGT GTGCTTGTTG AACACTTGTG CCAGCACTCA 
GGTGTCTGCA TCAATGCTGG CAACACGCAT TACTGTCAGT GCCCCCTGGG CTATACTGGG 
AGCTACTGTG AGGAGCAACT CGATGAGTGT GCGTCCAACC CCTGCCAGCA CGGGGCAACA 
TGCAGTGACT TCATTGGTGG ATACAGATGT GAGTGTGTCC CAGGCTATCA GGGTGTCAAC 
TGTGAGTATG AAGTGGATGA GTGCCAGAAT CAGCCCTGCC AGAATGGAGG CACCTGTATT 
GACCTTGTGA ACCATTTCAA GTGCTCTTGC CCACCAGGCA CTCGGGGCCT ACTCTGTGAA 
GAGAACATTG ATGACTGTGC CCGGGGTCCC CATTGCCTTA ATGGTGGTCA GTGCATGGAT 
AGGATTGGAG GCTACAGTTG TCGCTGCTTG CCTGGCTTTG CTGGGGAGCG TTGTGAGGGA 
GACATCAACG AGTGCCTCTC CAACCCCTGC AGCTCTGAGG GCAGCCTGGA CTGTATACAG 
CTCACCAATG ACTACCTGTG TGTTTGCCGT AGTGCCTTTA CTGGCCGGCA CTGTGAAACC 
TTCGTCGATG TGTGTCCCCA GATGCCCTGC CTGAATGGAG GGACTTGTGC TGTGGCCAGT 
AACATGCCTG ATGGTTTCAT TTGCCGTTGT CCCCCGGGAT TTTCTGGGGC AAGGTGCCAG 
AGCAGCTGTG GACAAGTGAA ATGTAGGAAA GGGGAGCAGT GTGTGCACAC CGCCTCTGGA 
CCCCGCTGCT TCTGCCCCAG TCCCCGGGAC TGCGAGTCAG GCTGTGCCAG TAGCCCCTGC 
CAGCACGGGG GCAGCTGCCA CCCTCAGCGC CAGCCTCCTT ATTACTCCTG CCAGTGTGCC 
CCACCATTCT CGGGTAGCCG CTGTGAACTC TACACGGCAC CCCCCAGCAC CCCTCCTGCC 
ACCTGTCTGA GCCAGTATTG TGCCGACAAA GCTCGGGATG GCGTCTGTGA TGAGGCCTGC 
AACAGCCATG CCTGCCAGTG GGATGGGGGT GACTGTTCTC TCACCATGGA GAACCCCTGG 
GCCAACTGCT CCTCCCCACT TCCCTGCTGG GATTATATCA ACAACCAGTG TGATGAGCTG 
TGCAACACGG TCGAGTGCCT GTTTGACAAC TTTGAATGCC AGGGGAACAG CAAGACATGC 
AAGTATGACA AATACTGTGC AGACCACTTC AAAGACAACC ACTGTGACCA GGGGTGCAAC 
AGTGAGGAGT GTGGTTGGGA TGGGCTGGAC TGTGCTGCTG ACCAACCTGA GAACCTGGCA 
GAAGGTACCC TGGTTATTGT GGTATTGATG CCACCTGAAC AACTGCTCCA GGATGCTCGC 
AGCTTCTTGC GGGCACTGGG TACCCTGCTG CACACCAACC TGCGCATTAA GCGGGACTCC 
CAGGGGGAAC TCATGGTGTA CCCCTATTAT GGTGAGAAGT CAGCTGCTAT GAAGAAACAG 
AGGATGACAC GCAGGTCCCT TCCTGGTGAA CAAGAACAGG AGGTGGCTGG CTCTAAAGTC 
TTTCTGGAAA TTGACAACCG CCAGTGTGTT CAAGACTCAG ACCACTGCTT CAAGAACACG 
GATGCAGCAG CAGCTCTCCT GGCCTCTCAC GCCATACAGG GGACCCTGTC ATACCCTCTT 
GTGTCTGTCG TCAGTGAATC CCTGACTCCA GAACGCACTC AGCTCCTCTA TCTCCTTGCT 
GTTGCTGTTG TCATCATTCT GTTTATTATT CTGCTGGGGG TAATCATGGC AAAACGAAAG 
CGTAAGCATG GCTCTCTCTG GCTGCCTGAA GGTTTCACTC TTCGCCGAGA TGCAAGCAAT 
CACAAGCGTC GTGAGCCAGT GGGACAGGAT GCTGTGGGGC TAAAAAATCT CTCAGTGCAA 
GTCTCAGAAG CTAACCTAAT TGGTTCTGGA ACAAGTGAAC ACTGGGTCGA TGATGAAGGG 
CCCCAGCCAA AGAAAGTAAA GGCTGAAGAT GAGGCCTTAC TCTCAGAAGA AGATGACCCC 
ATTGATCGAC GGCCATGGAC ACAGCAGCAC CTTGAAGCCG CAGACATCCG TAGGACACCA 
TCGCTGGCTC TCACCCCTCC TCAGGCAGAG CAGGAGGTGG ATGTGTTAGA TGTGAATGTC 
CGTGGCCCAG ATGGCTGCAC CCCATTGATG TTGGCTTCTC TCCGAGGAGG CAGCTCAGAT 
TTGAGTGATG AAGATGAAGA TGCAGAGGAC TCTTCTGCTA ACATCATCAC AGACTTGGTC 
TACCAGGGTG CCAGCCTCCA GGCCCAGACA GACCGGACTG GTGAGATGGC CCTGCACCTT 
GCAGCCCGCT ACTCACGGGC TGATGCTGCC AAGCGTCTCC TGGATGCAGG TGCAGATGCC 
AATGCCCAGG ACAACATGGG CCGCTGCCCA CTCCATGCTG CAGTGGCAGC TGATGCCCAA 
GGTGTCTTCC AGATTCTGAT TCGCAACCGA GTAACTGATC TAGATGCCAG GATGAATGAT 
GGTACTACAC CCCTGATCCT GGCTGCCCGC CTGGCTGTGG AGGGAATGGT GGCAGAACTG 
ATCAACTGCC AAGCGGATGT GAATGCAGTG GATGACCATG GAAAATCTGC TCTTCACTGG 
GCAGCTGCTG TCAATAATGT GGAGGCAACT CTTTTGTTGT TGAAAAATGG AGCCAACCGA 
GACATGCAGG ACAACAAGGA AGAGACACCT CTGTTTCTTG CTGCCCGGGA GGGGAGCTAT 
GAAGCAGCCA AGATCCTGTT AGACCATTTT GCCAATCGAG ACATCACAGA CCATATGGAT 
CGTCTTCCCC GGGATGTGGC TCGGGATCGC ATGCACCATG ACATTGTGCG CCTTCTGGAT 
GAATACAATG TGACCCCAAG CCCTCCAGGC ACCGTGTTGA CTTCTGCTCT CTCACCTGTC 
ATCTGTGGGC CCAACAGATC TTTCCTCAGC CTGAAGCACA CCCCAATGGG CAAGAAGTCT 
AGACGGCCCA GTGCCAAGAG TACCATGCCT ACTAGCCTCC CTAACCTTGC CAAGGAGGCA 
AAGGATGCCA AGGGTAGTAG GAGGAAGAAG TCTCTGAGTG AGAAGGTCCA ACTGTCTGAG 
AGTTCAGTAA CTTTATCCCC TGTTGATTCC CTAGAATCTC CTCACACGTA TGTTTCCGAC 
ACCACATCCT CTCCAATGAT TACATCCCCT GGGATCTTAC AGGCCTCACC CAACCCTATG 
TTGGCCACTG CCGCCCCTCC TGCCCCAGTC CATGCCCAGC ATGCACTATC TTTTTCTAAC 
CTTCATGAAA TGCAGCCTTT GGCACATGGG GCCAGCACTG TGCTTCCCTC AGTGAGCCAG 
TTGCTATCCC ACCACCACAT TGTGTCTCCA GGCAGTGGCA GTGCTGGAAG CTTGAGTAGG 
CTCCATCCAG TCCCAGTCCC AGCAGATTGG ATGAACCGCA TGGAGGTGAA TGAGACCCAG 
TACAATGAGA TGTTTGGTAT GGTCCTGGCT CCAGCTGAGG GCACCCATCC TGGCATAGCT 
CCCCAGAGCA GGCCACCTGA AGGGAAGCAC ATAACCACCC CTCGGGAGCC CTTGCCCCCC 
ATTGTGACTT TCCAGCTCAT CCCTAAAGGC AGTATTGCCC AACCAGCGGG GGCTCCCCAG 
CCTCAGTCCA CCTGCCCTCC AGCTGTTGCG GGCCCCCTGC CCACCATGTA CCAGATTCCA 
GAAATGGCCC GTTTGCCCAG TGTGGCTTTC CCCACTGCCA TGATGCCCCA GCAGGACGGG 
CAGGTAGCTC AGACCATTCT CCCAGCCTAT CATCCTTTCC CAGCCTCTGT GGGCAAGTAC 
CCCACACCCC CTTCACAGCA CAGTTATGCT TCCTCAAATG CTGCTGAGCG AACACCCAGT 
CACAGTGGTC ACCTCCAGGG TGAGCATCCC TACCTGACAC CATCCCCAGA GTCTCCTGAC 
CAGTGGTCAA GTTCATCACC CCACTCTGCT TCTGACTGGT CAGATGTGAC CACCAGCCCT 
ACCCCTGGGG GTGCTGGAGG AGGTCAGCGG GGACCTGGGA CACACATGTC TGAGCCACCA 
CACAACAACA TGCAGGTTTA TGCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NOTCH2 ( XM_016924549.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016780038.1 Pan troglodytes notch 2 (NOTCH2), transcript variant X4, mRNA.

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CloneID	OPc59962
Clone ID Related Accession (Same CDS sequence)	XM_016924542.1 , XM_016924549.1
Accession Version	XM_016924549.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7167bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	neurogenic locus notch homolog protein 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006468.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGGGGA AAGCCATGTG CCGATGTGCC TCAGGGTTTA CAGGAGAGGA CTGCCAGTAC 
TCGACATCTC ATCCATGCTT TGTGTCTCGA CCCTGCCTGA ATGGCGGCAC ATGCCATATG 
CTCAGCCGGG ATACCTATGA GTGCACCTGT CAAGTCGGGT TTACAGGTAA GGAGTGCCAA 
TGGACCGATG CCTGCCTGTC TCATCCCTGT GCAAATGGAA GTACCTGTAC CACTGTGGCC 
AACCAGTTCT CCTGCAAATG CCTCACAGGC TTCACAGGGC AGAAGTGTGA GACTGATGTC 
AATGAGTGTG ACATTCCAGG ACACTGCCAG CATGGTGGCA CCTGCCTCAA CCTGCCTGGT 
TCCTACCAGT GCCAGTGCCC TCAGGGCTTC ACAGGCCAGT ACTGTGACAG CCTGTATGTG 
CCCTGTGCAC CCTCGCCTTG TGTCAATGGA GGCACCTGTC GGCAGACTGG TGACTTCACT 
TTTGAGTGCA ACTGCCTTCC AGGTTTTGAA GGGAGCACCT GTGAGAGGAA TATTGATGAC 
TGCCCTAACC ACAGGTGTCA GAATGGAGGG GTTTGTGTGG ATGGGGTCAA CACTTACAAC 
TGCCGCTGTC CCCCACAATG GACAGGACAG TTCTGCACAG AGGATGTGGA TGAATGCCTG 
CTGCAGCCCA ATGCCTGTCA AAATGGGGGC ACCTGTGCCA ACCGCAATGG AGGCTATGGC 
TGTGTATGTG TCAACGGCTG GAGTGGAGAT GACTGCAGTG AGAACATTGA TGATTGTGCC 
TTCGCCTCCT GTACTCCAGG CTCCACCTGC ATCGACCGTG TGGCCTCCTT CTCTTGCATG 
TGCCCAGAGG GGAAGGCAGG TCTCCTGTGT CATCTGGATG ATGCATGCAT CAGCAATCCT 
TGCCACAAGG GGGCACTGTG TGACACCAAC CCCCTAAATG GGCAATATAT TTGCACCTGC 
CCACAAGGCT ACAAAGGGGC TGACTGCACA GAAGATGTGG ATGAATGTTC CATGGCCAAT 
AGCAATCCTT GTGAGCATGC AGGAAAATGT GTGAACACGG ATGGCGCCTT CCACTGTGAG 
TGTCTGAAGG GTTATGCAGG ACCTCGTTGT GAGATGGACA TCAATGAGTG CCATTCAGAC 
CCCTGCCAGA ATGATGCTAC CTGTCTGGAT AAGATTGGAG GCTTCACATG TCTGTGCATG 
CCAGGTTTCA AAGGTGTGCA TTGTGAATTA GAAATAAATG AATGTCAGAG CAACCCTTGT 
GTGAACAATG GGCAGTGTGT GGATAAAGTC AATCGTTTCC AGTGCCTGTG TCCTCCTGGT 
TTCACTGGGC CAGTTTGCCA GATTGATATT GATGACTGTT CCAGTACTCC GTGTCTGAAT 
GGGGCAAAGT GTATCGATCA CCCGAATGGC TATGAATGCC AGTGTGCCAC AGGTTTCACT 
GGTGTGTTGT GTGAGGAGAA CATTGACAAC TGTGACCCCG ATCCTTGCCA CCATGGTCAG 
TGTCAGGATG GTATTGATTC CTACACCTGC ATCTGCAATC CCGGGTACAT GGGCGCCATC 
TGCAGTGACC AGATTGATGA ATGTTACAGC AGCCCTTGCC TGAACGATGG TCGCTGCATT 
GACCTGGTCA ATGGCTACCA GTGCAACTGC CAGCCGGGCA CGTCAGGGGT TAATTGTGAA 
ATTAATTTTG ATGACTGTGC AAGTAACCCT TGTATCCATG GAAACTGTAT GGATGGCATT 
AATCGCTACA GTTGTGTCTG CTCACCAGGA TTCACAGGGC AGAGATGTAA CATTGACATT 
GATGAGTGTG CCTCCAATCC CTGTCGCAAG GGTGCAACAT GTATCAACGG TGTGAATGGT 
TTCCGCTGTA TATGCCCCGA GGGACCCCAT CACCCCAGCT GCTACTCACA GGTGAACGAA 
TGCCTGAGCA ATCCCTGCAT CCATGGAAAC TGTACTGGAG GTCTCAGTGG ATATAAGTGT 
CTCTGTGATG CAGGCTGGGT TGGCATCAAC TGTGAAGTGG ACAAAAATGA ATGCCTTTCG 
AATCCATGCC AGAATGGAGG AACTTGTGAC AATCTGGTGA ATGGATACAG GTGTACTTGC 
AAGAAGGGCT TTAAAGGCTA TAACTGCCAG GTGAATATTG ATGAATGTGC CTCAAATCCA 
TGCCTGAACC AAGGAACCTG CTTTGATGAC ATAAGTGGCT ACACTTGCCA CTGTGTGCTG 
CCATACACAG GCAAGAATTG TCAGACAGTA TTGGCTCCCT GTTCCCCAAA CCCTTGTGAG 
AATGCTGCTG TTTGCAAAGA GTCACCAAAT TTTGAGAGTT ATACTTGCTT GTGTGCTCCT 
GGCTGGCAAG GTCAGCGGTG TACCATTGAC ATTGACGAGT GTATCTCCAA GCCCTGCATG 
AACCATGGTC TCTGCCATAA CACCCAGGGC AGCTACATGT GTGAATGTCC ACCAGGCTTC 
AGTGGTATGG ACTGTGAGGA GGACATTGAT GACTGCCTTG CCAATCCTTG CCAGAATGGA 
GGTTCCTGTG TGGATGGAGT GAATACTTTC TCCTGCCTCT GCCTTCCGGG TTTCACTGGG 
GATAAGTGCC AGACAGACAT GAATGAGTGT CTGAGTGAAC CCTGTAAGAA TGGAGGGACC 
TGCTCTGACT ACGTCAACAG TTACACTTGC AAGTGCCAGG CAGGATTTGA TGGAGTCCAT 
TGTGAGAACA ACATCAATGA GTGCACTGAG AGCTCCTGTT TCAATGGTGG CACATGTGTT 
GATGGGATTA ACTCCTTCTC TTGCTTGTGC CCTGTGGGTT TCACTGGATC CTTCTGCCTC 
CATGAGATCA ATGAATGCAG CTCTCATCCA TGCCTCAATG AGGGAACGTG TGTTGATGGC 
CTGGGTACCT ACCGCTGCAG CTGCCCCCTG GGCTACACTG GGAAAAACTG TCAGACCCTG 
GTGAACCTCT GCAGTCGGTC TCCATGTAAA AACAAAGGTA CTTGCGTTCA GAAAAAAGCA 
GAGTCCCAGT GCCTATGTCC ATCTGGATGG GCTGGTGCCT ATTGTGACGT GCCCAATGTC 
TCTTGTGACA TAGCAGCCTC CAGGAGAGGT GTGCTTGTTG AACACTTGTG CCAGCACTCA 
GGTGTCTGCA TCAATGCTGG CAACACGCAT TACTGTCAGT GCCCCCTGGG CTATACTGGG 
AGCTACTGTG AGGAGCAACT CGATGAGTGT GCGTCCAACC CCTGCCAGCA CGGGGCAACA 
TGCAGTGACT TCATTGGTGG ATACAGATGT GAGTGTGTCC CAGGCTATCA GGGTGTCAAC 
TGTGAGTATG AAGTGGATGA GTGCCAGAAT CAGCCCTGCC AGAATGGAGG CACCTGTATT 
GACCTTGTGA ACCATTTCAA GTGCTCTTGC CCACCAGGCA CTCGGGGCCT ACTCTGTGAA 
GAGAACATTG ATGACTGTGC CCGGGGTCCC CATTGCCTTA ATGGTGGTCA GTGCATGGAT 
AGGATTGGAG GCTACAGTTG TCGCTGCTTG CCTGGCTTTG CTGGGGAGCG TTGTGAGGGA 
GACATCAACG AGTGCCTCTC CAACCCCTGC AGCTCTGAGG GCAGCCTGGA CTGTATACAG 
CTCACCAATG ACTACCTGTG TGTTTGCCGT AGTGCCTTTA CTGGCCGGCA CTGTGAAACC 
TTCGTCGATG TGTGTCCCCA GATGCCCTGC CTGAATGGAG GGACTTGTGC TGTGGCCAGT 
AACATGCCTG ATGGTTTCAT TTGCCGTTGT CCCCCGGGAT TTTCTGGGGC AAGGTGCCAG 
AGCAGCTGTG GACAAGTGAA ATGTAGGAAA GGGGAGCAGT GTGTGCACAC CGCCTCTGGA 
CCCCGCTGCT TCTGCCCCAG TCCCCGGGAC TGCGAGTCAG GCTGTGCCAG TAGCCCCTGC 
CAGCACGGGG GCAGCTGCCA CCCTCAGCGC CAGCCTCCTT ATTACTCCTG CCAGTGTGCC 
CCACCATTCT CGGGTAGCCG CTGTGAACTC TACACGGCAC CCCCCAGCAC CCCTCCTGCC 
ACCTGTCTGA GCCAGTATTG TGCCGACAAA GCTCGGGATG GCGTCTGTGA TGAGGCCTGC 
AACAGCCATG CCTGCCAGTG GGATGGGGGT GACTGTTCTC TCACCATGGA GAACCCCTGG 
GCCAACTGCT CCTCCCCACT TCCCTGCTGG GATTATATCA ACAACCAGTG TGATGAGCTG 
TGCAACACGG TCGAGTGCCT GTTTGACAAC TTTGAATGCC AGGGGAACAG CAAGACATGC 
AAGTATGACA AATACTGTGC AGACCACTTC AAAGACAACC ACTGTGACCA GGGGTGCAAC 
AGTGAGGAGT GTGGTTGGGA TGGGCTGGAC TGTGCTGCTG ACCAACCTGA GAACCTGGCA 
GAAGGTACCC TGGTTATTGT GGTATTGATG CCACCTGAAC AACTGCTCCA GGATGCTCGC 
AGCTTCTTGC GGGCACTGGG TACCCTGCTG CACACCAACC TGCGCATTAA GCGGGACTCC 
CAGGGGGAAC TCATGGTGTA CCCCTATTAT GGTGAGAAGT CAGCTGCTAT GAAGAAACAG 
AGGATGACAC GCAGGTCCCT TCCTGGTGAA CAAGAACAGG AGGTGGCTGG CTCTAAAGTC 
TTTCTGGAAA TTGACAACCG CCAGTGTGTT CAAGACTCAG ACCACTGCTT CAAGAACACG 
GATGCAGCAG CAGCTCTCCT GGCCTCTCAC GCCATACAGG GGACCCTGTC ATACCCTCTT 
GTGTCTGTCG TCAGTGAATC CCTGACTCCA GAACGCACTC AGCTCCTCTA TCTCCTTGCT 
GTTGCTGTTG TCATCATTCT GTTTATTATT CTGCTGGGGG TAATCATGGC AAAACGAAAG 
CGTAAGCATG GCTCTCTCTG GCTGCCTGAA GGTTTCACTC TTCGCCGAGA TGCAAGCAAT 
CACAAGCGTC GTGAGCCAGT GGGACAGGAT GCTGTGGGGC TAAAAAATCT CTCAGTGCAA 
GTCTCAGAAG CTAACCTAAT TGGTTCTGGA ACAAGTGAAC ACTGGGTCGA TGATGAAGGG 
CCCCAGCCAA AGAAAGTAAA GGCTGAAGAT GAGGCCTTAC TCTCAGAAGA AGATGACCCC 
ATTGATCGAC GGCCATGGAC ACAGCAGCAC CTTGAAGCCG CAGACATCCG TAGGACACCA 
TCGCTGGCTC TCACCCCTCC TCAGGCAGAG CAGGAGGTGG ATGTGTTAGA TGTGAATGTC 
CGTGGCCCAG ATGGCTGCAC CCCATTGATG TTGGCTTCTC TCCGAGGAGG CAGCTCAGAT 
TTGAGTGATG AAGATGAAGA TGCAGAGGAC TCTTCTGCTA ACATCATCAC AGACTTGGTC 
TACCAGGGTG CCAGCCTCCA GGCCCAGACA GACCGGACTG GTGAGATGGC CCTGCACCTT 
GCAGCCCGCT ACTCACGGGC TGATGCTGCC AAGCGTCTCC TGGATGCAGG TGCAGATGCC 
AATGCCCAGG ACAACATGGG CCGCTGCCCA CTCCATGCTG CAGTGGCAGC TGATGCCCAA 
GGTGTCTTCC AGATTCTGAT TCGCAACCGA GTAACTGATC TAGATGCCAG GATGAATGAT 
GGTACTACAC CCCTGATCCT GGCTGCCCGC CTGGCTGTGG AGGGAATGGT GGCAGAACTG 
ATCAACTGCC AAGCGGATGT GAATGCAGTG GATGACCATG GAAAATCTGC TCTTCACTGG 
GCAGCTGCTG TCAATAATGT GGAGGCAACT CTTTTGTTGT TGAAAAATGG AGCCAACCGA 
GACATGCAGG ACAACAAGGA AGAGACACCT CTGTTTCTTG CTGCCCGGGA GGGGAGCTAT 
GAAGCAGCCA AGATCCTGTT AGACCATTTT GCCAATCGAG ACATCACAGA CCATATGGAT 
CGTCTTCCCC GGGATGTGGC TCGGGATCGC ATGCACCATG ACATTGTGCG CCTTCTGGAT 
GAATACAATG TGACCCCAAG CCCTCCAGGC ACCGTGTTGA CTTCTGCTCT CTCACCTGTC 
ATCTGTGGGC CCAACAGATC TTTCCTCAGC CTGAAGCACA CCCCAATGGG CAAGAAGTCT 
AGACGGCCCA GTGCCAAGAG TACCATGCCT ACTAGCCTCC CTAACCTTGC CAAGGAGGCA 
AAGGATGCCA AGGGTAGTAG GAGGAAGAAG TCTCTGAGTG AGAAGGTCCA ACTGTCTGAG 
AGTTCAGTAA CTTTATCCCC TGTTGATTCC CTAGAATCTC CTCACACGTA TGTTTCCGAC 
ACCACATCCT CTCCAATGAT TACATCCCCT GGGATCTTAC AGGCCTCACC CAACCCTATG 
TTGGCCACTG CCGCCCCTCC TGCCCCAGTC CATGCCCAGC ATGCACTATC TTTTTCTAAC 
CTTCATGAAA TGCAGCCTTT GGCACATGGG GCCAGCACTG TGCTTCCCTC AGTGAGCCAG 
TTGCTATCCC ACCACCACAT TGTGTCTCCA GGCAGTGGCA GTGCTGGAAG CTTGAGTAGG 
CTCCATCCAG TCCCAGTCCC AGCAGATTGG ATGAACCGCA TGGAGGTGAA TGAGACCCAG 
TACAATGAGA TGTTTGGTAT GGTCCTGGCT CCAGCTGAGG GCACCCATCC TGGCATAGCT 
CCCCAGAGCA GGCCACCTGA AGGGAAGCAC ATAACCACCC CTCGGGAGCC CTTGCCCCCC 
ATTGTGACTT TCCAGCTCAT CCCTAAAGGC AGTATTGCCC AACCAGCGGG GGCTCCCCAG 
CCTCAGTCCA CCTGCCCTCC AGCTGTTGCG GGCCCCCTGC CCACCATGTA CCAGATTCCA 
GAAATGGCCC GTTTGCCCAG TGTGGCTTTC CCCACTGCCA TGATGCCCCA GCAGGACGGG 
CAGGTAGCTC AGACCATTCT CCCAGCCTAT CATCCTTTCC CAGCCTCTGT GGGCAAGTAC 
CCCACACCCC CTTCACAGCA CAGTTATGCT TCCTCAAATG CTGCTGAGCG AACACCCAGT 
CACAGTGGTC ACCTCCAGGG TGAGCATCCC TACCTGACAC CATCCCCAGA GTCTCCTGAC 
CAGTGGTCAA GTTCATCACC CCACTCTGCT TCTGACTGGT CAGATGTGAC CACCAGCCCT 
ACCCCTGGGG GTGCTGGAGG AGGTCAGCGG GGACCTGGGA CACACATGTC TGAGCCACCA 
CACAACAACA TGCAGGTTTA TGCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016780038.1
CDS	3120..10286
Translation	MLGKAMCRCASGFTGEDCQYSTSHPCFVSRPCLNGGTCHMLSRDTYECTCQVGFTGKECQWTDACLSHPCANGSTCTTVANQFSCKCLTGFTGQKCETDVNECDIPGHCQHGGTCLNLPGSYQCQCPQGFTGQYCDSLYVPCAPSPCVNGGTCRQTGDFTFECNCLPGFEGSTCERNIDDCPNHRCQNGGVCVDGVNTYNCRCPPQWTGQFCTEDVDECLLQPNACQNGGTCANRNGGYGCVCVNGWSGDDCSENIDDCAFASCTPGSTCIDRVASFSCMCPEGKAGLLCHLDDACISNPCHKGALCDTNPLNGQYICTCPQGYKGADCTEDVDECSMANSNPCEHAGKCVNTDGAFHCECLKGYAGPRCEMDINECHSDPCQNDATCLDKIGGFTCLCMPGFKGVHCELEINECQSNPCVNNGQCVDKVNRFQCLCPPGFTGPVCQIDIDDCSSTPCLNGAKCIDHPNGYECQCATGFTGVLCEENIDNCDPDPCHHGQCQDGIDSYTCICNPGYMGAICSDQIDECYSSPCLNDGRCIDLVNGYQCNCQPGTSGVNCEINFDDCASNPCIHGNCMDGINRYSCVCSPGFTGQRCNIDIDECASNPCRKGATCINGVNGFRCICPEGPHHPSCYSQVNECLSNPCIHGNCTGGLSGYKCLCDAGWVGINCEVDKNECLSNPCQNGGTCDNLVNGYRCTCKKGFKGYNCQVNIDECASNPCLNQGTCFDDISGYTCHCVLPYTGKNCQTVLAPCSPNPCENAAVCKESPNFESYTCLCAPGWQGQRCTIDIDECISKPCMNHGLCHNTQGSYMCECPPGFSGMDCEEDIDDCLANPCQNGGSCVDGVNTFSCLCLPGFTGDKCQTDMNECLSEPCKNGGTCSDYVNSYTCKCQAGFDGVHCENNINECTESSCFNGGTCVDGINSFSCLCPVGFTGSFCLHEINECSSHPCLNEGTCVDGLGTYRCSCPLGYTGKNCQTLVNLCSRSPCKNKGTCVQKKAESQCLCPSGWAGAYCDVPNVSCDIAASRRGVLVEHLCQHSGVCINAGNTHYCQCPLGYTGSYCEEQLDECASNPCQHGATCSDFIGGYRCECVPGYQGVNCEYEVDECQNQPCQNGGTCIDLVNHFKCSCPPGTRGLLCEENIDDCARGPHCLNGGQCMDRIGGYSCRCLPGFAGERCEGDINECLSNPCSSEGSLDCIQLTNDYLCVCRSAFTGRHCETFVDVCPQMPCLNGGTCAVASNMPDGFICRCPPGFSGARCQSSCGQVKCRKGEQCVHTASGPRCFCPSPRDCESGCASSPCQHGGSCHPQRQPPYYSCQCAPPFSGSRCELYTAPPSTPPATCLSQYCADKARDGVCDEACNSHACQWDGGDCSLTMENPWANCSSPLPCWDYINNQCDELCNTVECLFDNFECQGNSKTCKYDKYCADHFKDNHCDQGCNSEECGWDGLDCAADQPENLAEGTLVIVVLMPPEQLLQDARSFLRALGTLLHTNLRIKRDSQGELMVYPYYGEKSAAMKKQRMTRRSLPGEQEQEVAGSKVFLEIDNRQCVQDSDHCFKNTDAAAALLASHAIQGTLSYPLVSVVSESLTPERTQLLYLLAVAVVIILFIILLGVIMAKRKRKHGSLWLPEGFTLRRDASNHKRREPVGQDAVGLKNLSVQVSEANLIGSGTSEHWVDDEGPQPKKVKAEDEALLSEEDDPIDRRPWTQQHLEAADIRRTPSLALTPPQAEQEVDVLDVNVRGPDGCTPLMLASLRGGSSDLSDEDEDAEDSSANIITDLVYQGASLQAQTDRTGEMALHLAARYSRADAAKRLLDAGADANAQDNMGRCPLHAAVAADAQGVFQILIRNRVTDLDARMNDGTTPLILAARLAVEGMVAELINCQADVNAVDDHGKSALHWAAAVNNVEATLLLLKNGANRDMQDNKEETPLFLAAREGSYEAAKILLDHFANRDITDHMDRLPRDVARDRMHHDIVRLLDEYNVTPSPPGTVLTSALSPVICGPNRSFLSLKHTPMGKKSRRPSAKSTMPTSLPNLAKEAKDAKGSRRKKSLSEKVQLSESSVTLSPVDSLESPHTYVSDTTSSPMITSPGILQASPNPMLATAAPPAPVHAQHALSFSNLHEMQPLAHGASTVLPSVSQLLSHHHIVSPGSGSAGSLSRLHPVPVPADWMNRMEVNETQYNEMFGMVLAPAEGTHPGIAPQSRPPEGKHITTPREPLPPIVTFQLIPKGSIAQPAGAPQPQSTCPPAVAGPLPTMYQIPEMARLPSVAFPTAMMPQQDGQVAQTILPAYHPFPASVGKYPTPPSQHSYASSNAAERTPSHSGHLQGEHPYLTPSPESPDQWSSSSPHSASDWSDVTTSPTPGGAGGGQRGPGTHMSEPPHNNMQVYA

Target ORF information:

RefSeq Version	XM_016924549.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes notch 2 (NOTCH2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016924549.1

ORF Insert Sequence:

ATGCTGGGGA AAGCCATGTG CCGATGTGCC TCAGGGTTTA CAGGAGAGGA CTGCCAGTAC 
TCGACATCTC ATCCATGCTT TGTGTCTCGA CCCTGCCTGA ATGGCGGCAC ATGCCATATG 
CTCAGCCGGG ATACCTATGA GTGCACCTGT CAAGTCGGGT TTACAGGTAA GGAGTGCCAA 
TGGACCGATG CCTGCCTGTC TCATCCCTGT GCAAATGGAA GTACCTGTAC CACTGTGGCC 
AACCAGTTCT CCTGCAAATG CCTCACAGGC TTCACAGGGC AGAAGTGTGA GACTGATGTC 
AATGAGTGTG ACATTCCAGG ACACTGCCAG CATGGTGGCA CCTGCCTCAA CCTGCCTGGT 
TCCTACCAGT GCCAGTGCCC TCAGGGCTTC ACAGGCCAGT ACTGTGACAG CCTGTATGTG 
CCCTGTGCAC CCTCGCCTTG TGTCAATGGA GGCACCTGTC GGCAGACTGG TGACTTCACT 
TTTGAGTGCA ACTGCCTTCC AGGTTTTGAA GGGAGCACCT GTGAGAGGAA TATTGATGAC 
TGCCCTAACC ACAGGTGTCA GAATGGAGGG GTTTGTGTGG ATGGGGTCAA CACTTACAAC 
TGCCGCTGTC CCCCACAATG GACAGGACAG TTCTGCACAG AGGATGTGGA TGAATGCCTG 
CTGCAGCCCA ATGCCTGTCA AAATGGGGGC ACCTGTGCCA ACCGCAATGG AGGCTATGGC 
TGTGTATGTG TCAACGGCTG GAGTGGAGAT GACTGCAGTG AGAACATTGA TGATTGTGCC 
TTCGCCTCCT GTACTCCAGG CTCCACCTGC ATCGACCGTG TGGCCTCCTT CTCTTGCATG 
TGCCCAGAGG GGAAGGCAGG TCTCCTGTGT CATCTGGATG ATGCATGCAT CAGCAATCCT 
TGCCACAAGG GGGCACTGTG TGACACCAAC CCCCTAAATG GGCAATATAT TTGCACCTGC 
CCACAAGGCT ACAAAGGGGC TGACTGCACA GAAGATGTGG ATGAATGTTC CATGGCCAAT 
AGCAATCCTT GTGAGCATGC AGGAAAATGT GTGAACACGG ATGGCGCCTT CCACTGTGAG 
TGTCTGAAGG GTTATGCAGG ACCTCGTTGT GAGATGGACA TCAATGAGTG CCATTCAGAC 
CCCTGCCAGA ATGATGCTAC CTGTCTGGAT AAGATTGGAG GCTTCACATG TCTGTGCATG 
CCAGGTTTCA AAGGTGTGCA TTGTGAATTA GAAATAAATG AATGTCAGAG CAACCCTTGT 
GTGAACAATG GGCAGTGTGT GGATAAAGTC AATCGTTTCC AGTGCCTGTG TCCTCCTGGT 
TTCACTGGGC CAGTTTGCCA GATTGATATT GATGACTGTT CCAGTACTCC GTGTCTGAAT 
GGGGCAAAGT GTATCGATCA CCCGAATGGC TATGAATGCC AGTGTGCCAC AGGTTTCACT 
GGTGTGTTGT GTGAGGAGAA CATTGACAAC TGTGACCCCG ATCCTTGCCA CCATGGTCAG 
TGTCAGGATG GTATTGATTC CTACACCTGC ATCTGCAATC CCGGGTACAT GGGCGCCATC 
TGCAGTGACC AGATTGATGA ATGTTACAGC AGCCCTTGCC TGAACGATGG TCGCTGCATT 
GACCTGGTCA ATGGCTACCA GTGCAACTGC CAGCCGGGCA CGTCAGGGGT TAATTGTGAA 
ATTAATTTTG ATGACTGTGC AAGTAACCCT TGTATCCATG GAAACTGTAT GGATGGCATT 
AATCGCTACA GTTGTGTCTG CTCACCAGGA TTCACAGGGC AGAGATGTAA CATTGACATT 
GATGAGTGTG CCTCCAATCC CTGTCGCAAG GGTGCAACAT GTATCAACGG TGTGAATGGT 
TTCCGCTGTA TATGCCCCGA GGGACCCCAT CACCCCAGCT GCTACTCACA GGTGAACGAA 
TGCCTGAGCA ATCCCTGCAT CCATGGAAAC TGTACTGGAG GTCTCAGTGG ATATAAGTGT 
CTCTGTGATG CAGGCTGGGT TGGCATCAAC TGTGAAGTGG ACAAAAATGA ATGCCTTTCG 
AATCCATGCC AGAATGGAGG AACTTGTGAC AATCTGGTGA ATGGATACAG GTGTACTTGC 
AAGAAGGGCT TTAAAGGCTA TAACTGCCAG GTGAATATTG ATGAATGTGC CTCAAATCCA 
TGCCTGAACC AAGGAACCTG CTTTGATGAC ATAAGTGGCT ACACTTGCCA CTGTGTGCTG 
CCATACACAG GCAAGAATTG TCAGACAGTA TTGGCTCCCT GTTCCCCAAA CCCTTGTGAG 
AATGCTGCTG TTTGCAAAGA GTCACCAAAT TTTGAGAGTT ATACTTGCTT GTGTGCTCCT 
GGCTGGCAAG GTCAGCGGTG TACCATTGAC ATTGACGAGT GTATCTCCAA GCCCTGCATG 
AACCATGGTC TCTGCCATAA CACCCAGGGC AGCTACATGT GTGAATGTCC ACCAGGCTTC 
AGTGGTATGG ACTGTGAGGA GGACATTGAT GACTGCCTTG CCAATCCTTG CCAGAATGGA 
GGTTCCTGTG TGGATGGAGT GAATACTTTC TCCTGCCTCT GCCTTCCGGG TTTCACTGGG 
GATAAGTGCC AGACAGACAT GAATGAGTGT CTGAGTGAAC CCTGTAAGAA TGGAGGGACC 
TGCTCTGACT ACGTCAACAG TTACACTTGC AAGTGCCAGG CAGGATTTGA TGGAGTCCAT 
TGTGAGAACA ACATCAATGA GTGCACTGAG AGCTCCTGTT TCAATGGTGG CACATGTGTT 
GATGGGATTA ACTCCTTCTC TTGCTTGTGC CCTGTGGGTT TCACTGGATC CTTCTGCCTC 
CATGAGATCA ATGAATGCAG CTCTCATCCA TGCCTCAATG AGGGAACGTG TGTTGATGGC 
CTGGGTACCT ACCGCTGCAG CTGCCCCCTG GGCTACACTG GGAAAAACTG TCAGACCCTG 
GTGAACCTCT GCAGTCGGTC TCCATGTAAA AACAAAGGTA CTTGCGTTCA GAAAAAAGCA 
GAGTCCCAGT GCCTATGTCC ATCTGGATGG GCTGGTGCCT ATTGTGACGT GCCCAATGTC 
TCTTGTGACA TAGCAGCCTC CAGGAGAGGT GTGCTTGTTG AACACTTGTG CCAGCACTCA 
GGTGTCTGCA TCAATGCTGG CAACACGCAT TACTGTCAGT GCCCCCTGGG CTATACTGGG 
AGCTACTGTG AGGAGCAACT CGATGAGTGT GCGTCCAACC CCTGCCAGCA CGGGGCAACA 
TGCAGTGACT TCATTGGTGG ATACAGATGT GAGTGTGTCC CAGGCTATCA GGGTGTCAAC 
TGTGAGTATG AAGTGGATGA GTGCCAGAAT CAGCCCTGCC AGAATGGAGG CACCTGTATT 
GACCTTGTGA ACCATTTCAA GTGCTCTTGC CCACCAGGCA CTCGGGGCCT ACTCTGTGAA 
GAGAACATTG ATGACTGTGC CCGGGGTCCC CATTGCCTTA ATGGTGGTCA GTGCATGGAT 
AGGATTGGAG GCTACAGTTG TCGCTGCTTG CCTGGCTTTG CTGGGGAGCG TTGTGAGGGA 
GACATCAACG AGTGCCTCTC CAACCCCTGC AGCTCTGAGG GCAGCCTGGA CTGTATACAG 
CTCACCAATG ACTACCTGTG TGTTTGCCGT AGTGCCTTTA CTGGCCGGCA CTGTGAAACC 
TTCGTCGATG TGTGTCCCCA GATGCCCTGC CTGAATGGAG GGACTTGTGC TGTGGCCAGT 
AACATGCCTG ATGGTTTCAT TTGCCGTTGT CCCCCGGGAT TTTCTGGGGC AAGGTGCCAG 
AGCAGCTGTG GACAAGTGAA ATGTAGGAAA GGGGAGCAGT GTGTGCACAC CGCCTCTGGA 
CCCCGCTGCT TCTGCCCCAG TCCCCGGGAC TGCGAGTCAG GCTGTGCCAG TAGCCCCTGC 
CAGCACGGGG GCAGCTGCCA CCCTCAGCGC CAGCCTCCTT ATTACTCCTG CCAGTGTGCC 
CCACCATTCT CGGGTAGCCG CTGTGAACTC TACACGGCAC CCCCCAGCAC CCCTCCTGCC 
ACCTGTCTGA GCCAGTATTG TGCCGACAAA GCTCGGGATG GCGTCTGTGA TGAGGCCTGC 
AACAGCCATG CCTGCCAGTG GGATGGGGGT GACTGTTCTC TCACCATGGA GAACCCCTGG 
GCCAACTGCT CCTCCCCACT TCCCTGCTGG GATTATATCA ACAACCAGTG TGATGAGCTG 
TGCAACACGG TCGAGTGCCT GTTTGACAAC TTTGAATGCC AGGGGAACAG CAAGACATGC 
AAGTATGACA AATACTGTGC AGACCACTTC AAAGACAACC ACTGTGACCA GGGGTGCAAC 
AGTGAGGAGT GTGGTTGGGA TGGGCTGGAC TGTGCTGCTG ACCAACCTGA GAACCTGGCA 
GAAGGTACCC TGGTTATTGT GGTATTGATG CCACCTGAAC AACTGCTCCA GGATGCTCGC 
AGCTTCTTGC GGGCACTGGG TACCCTGCTG CACACCAACC TGCGCATTAA GCGGGACTCC 
CAGGGGGAAC TCATGGTGTA CCCCTATTAT GGTGAGAAGT CAGCTGCTAT GAAGAAACAG 
AGGATGACAC GCAGGTCCCT TCCTGGTGAA CAAGAACAGG AGGTGGCTGG CTCTAAAGTC 
TTTCTGGAAA TTGACAACCG CCAGTGTGTT CAAGACTCAG ACCACTGCTT CAAGAACACG 
GATGCAGCAG CAGCTCTCCT GGCCTCTCAC GCCATACAGG GGACCCTGTC ATACCCTCTT 
GTGTCTGTCG TCAGTGAATC CCTGACTCCA GAACGCACTC AGCTCCTCTA TCTCCTTGCT 
GTTGCTGTTG TCATCATTCT GTTTATTATT CTGCTGGGGG TAATCATGGC AAAACGAAAG 
CGTAAGCATG GCTCTCTCTG GCTGCCTGAA GGTTTCACTC TTCGCCGAGA TGCAAGCAAT 
CACAAGCGTC GTGAGCCAGT GGGACAGGAT GCTGTGGGGC TAAAAAATCT CTCAGTGCAA 
GTCTCAGAAG CTAACCTAAT TGGTTCTGGA ACAAGTGAAC ACTGGGTCGA TGATGAAGGG 
CCCCAGCCAA AGAAAGTAAA GGCTGAAGAT GAGGCCTTAC TCTCAGAAGA AGATGACCCC 
ATTGATCGAC GGCCATGGAC ACAGCAGCAC CTTGAAGCCG CAGACATCCG TAGGACACCA 
TCGCTGGCTC TCACCCCTCC TCAGGCAGAG CAGGAGGTGG ATGTGTTAGA TGTGAATGTC 
CGTGGCCCAG ATGGCTGCAC CCCATTGATG TTGGCTTCTC TCCGAGGAGG CAGCTCAGAT 
TTGAGTGATG AAGATGAAGA TGCAGAGGAC TCTTCTGCTA ACATCATCAC AGACTTGGTC 
TACCAGGGTG CCAGCCTCCA GGCCCAGACA GACCGGACTG GTGAGATGGC CCTGCACCTT 
GCAGCCCGCT ACTCACGGGC TGATGCTGCC AAGCGTCTCC TGGATGCAGG TGCAGATGCC 
AATGCCCAGG ACAACATGGG CCGCTGCCCA CTCCATGCTG CAGTGGCAGC TGATGCCCAA 
GGTGTCTTCC AGATTCTGAT TCGCAACCGA GTAACTGATC TAGATGCCAG GATGAATGAT 
GGTACTACAC CCCTGATCCT GGCTGCCCGC CTGGCTGTGG AGGGAATGGT GGCAGAACTG 
ATCAACTGCC AAGCGGATGT GAATGCAGTG GATGACCATG GAAAATCTGC TCTTCACTGG 
GCAGCTGCTG TCAATAATGT GGAGGCAACT CTTTTGTTGT TGAAAAATGG AGCCAACCGA 
GACATGCAGG ACAACAAGGA AGAGACACCT CTGTTTCTTG CTGCCCGGGA GGGGAGCTAT 
GAAGCAGCCA AGATCCTGTT AGACCATTTT GCCAATCGAG ACATCACAGA CCATATGGAT 
CGTCTTCCCC GGGATGTGGC TCGGGATCGC ATGCACCATG ACATTGTGCG CCTTCTGGAT 
GAATACAATG TGACCCCAAG CCCTCCAGGC ACCGTGTTGA CTTCTGCTCT CTCACCTGTC 
ATCTGTGGGC CCAACAGATC TTTCCTCAGC CTGAAGCACA CCCCAATGGG CAAGAAGTCT 
AGACGGCCCA GTGCCAAGAG TACCATGCCT ACTAGCCTCC CTAACCTTGC CAAGGAGGCA 
AAGGATGCCA AGGGTAGTAG GAGGAAGAAG TCTCTGAGTG AGAAGGTCCA ACTGTCTGAG 
AGTTCAGTAA CTTTATCCCC TGTTGATTCC CTAGAATCTC CTCACACGTA TGTTTCCGAC 
ACCACATCCT CTCCAATGAT TACATCCCCT GGGATCTTAC AGGCCTCACC CAACCCTATG 
TTGGCCACTG CCGCCCCTCC TGCCCCAGTC CATGCCCAGC ATGCACTATC TTTTTCTAAC 
CTTCATGAAA TGCAGCCTTT GGCACATGGG GCCAGCACTG TGCTTCCCTC AGTGAGCCAG 
TTGCTATCCC ACCACCACAT TGTGTCTCCA GGCAGTGGCA GTGCTGGAAG CTTGAGTAGG 
CTCCATCCAG TCCCAGTCCC AGCAGATTGG ATGAACCGCA TGGAGGTGAA TGAGACCCAG 
TACAATGAGA TGTTTGGTAT GGTCCTGGCT CCAGCTGAGG GCACCCATCC TGGCATAGCT 
CCCCAGAGCA GGCCACCTGA AGGGAAGCAC ATAACCACCC CTCGGGAGCC CTTGCCCCCC 
ATTGTGACTT TCCAGCTCAT CCCTAAAGGC AGTATTGCCC AACCAGCGGG GGCTCCCCAG 
CCTCAGTCCA CCTGCCCTCC AGCTGTTGCG GGCCCCCTGC CCACCATGTA CCAGATTCCA 
GAAATGGCCC GTTTGCCCAG TGTGGCTTTC CCCACTGCCA TGATGCCCCA GCAGGACGGG 
CAGGTAGCTC AGACCATTCT CCCAGCCTAT CATCCTTTCC CAGCCTCTGT GGGCAAGTAC 
CCCACACCCC CTTCACAGCA CAGTTATGCT TCCTCAAATG CTGCTGAGCG AACACCCAGT 
CACAGTGGTC ACCTCCAGGG TGAGCATCCC TACCTGACAC CATCCCCAGA GTCTCCTGAC 
CAGTGGTCAA GTTCATCACC CCACTCTGCT TCTGACTGGT CAGATGTGAC CACCAGCCCT 
ACCCCTGGGG GTGCTGGAGG AGGTCAGCGG GGACCTGGGA CACACATGTC TGAGCCACCA 
CACAACAACA TGCAGGTTTA TGCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NOTCH2 ( XM_024354924.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_024210692.1 Pan troglodytes notch 2 (NOTCH2), transcript variant X1, mRNA.

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CloneID	OPc92100
Clone ID Related Accession (Same CDS sequence)	XM_024354924.1
Accession Version	XM_024354924.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7416bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	neurogenic locus notch homolog protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036879.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGGAC ATCAAAGATC TGAAGGCCCA GCTGCAGAAT GCCAACAAGG TCATTCAGAA 
CCTCAAGAGC CAGCATTGCA GTGTCGAGAT GGCTATGAAC CCTGTGTAAA TGAAGGAATG 
TGTGTTACCT ACCACAATGG CACAGGATAC TGCAAATGTC CAGAAGGCTT CTTGGGGGAA 
TATTGTCAAC ATCGAGACCC CTGTGAGAAG AACCGCTGCC AGAATGGTGG GACTTGTGTG 
GCCCAGGCCA TGCTGGGGAA AGCCACGTGC CGATGTGCCT CAGGGTTTAC AGGAGAGGAC 
TGCCAGTACT CGACATCTCA TCCATGCTTT GTGTCTCGAC CCTGCCTGAA TGGCGGCACA 
TGCCATATGC TCAGCCGGGA TACCTATGAG TGCACCTGTC AAGTCGGGTT TACAGGTAAG 
GAGTGCCAAT GGACCGATGC CTGCCTGTCT CATCCCTGTG CAAATGGAAG TACCTGTACC 
ACTGTGGCCA ACCAGTTCTC CTGCAAATGC CTCACAGGCT TCACAGGGCA GAAGTGTGAG 
ACTGATGTCA ATGAGTGTGA CATTCCAGGA CACTGCCAGC ATGGTGGCAC CTGCCTCAAC 
CTGCCTGGTT CCTACCAGTG CCAGTGCCCT CAGGGCTTCA CAGGCCAGTA CTGTGACAGC 
CTGTATGTGC CCTGTGCACC CTCGCCTTGT GTCAATGGAG GCACCTGTCG GCAGACTGGT 
GACTTCACTT TTGAGTGCAG CTGCCTTCCA GGTTTTGAAG GGAGCACCTG TGAGAGGAAT 
ATTGATGACT GCCCTAACCA CAGGTGTCAG AATGGAGGGG TTTGTGTGGA TGGGGTCAAC 
ACTTACAACT GCCGCTGTCC CCCACAATGG ACAGGACAGT TCTGCACAGA GGATGTGGAT 
GAATGCCTGC TGCAGCCCAA TGCCTGTCAA AATGGGGGCA CCTGTGCCAA CCGCAATGGA 
GGCTATGGCT GTGTATGTGT CAACGGCTGG AGTGGAGATG ACTGCAGTGA GAACATTGAT 
GATTGTGCCT TCGCCTCCTG TACTCCAGGC TCCACCTGCA TCGACCGTGT GGCCTCCTTC 
TCTTGCATGT GCCCAGAGGG GAAGGCAGGT CTCCTGTGTC ATCTGGATGA TGCATGCATC 
AGCAATCCTT GCCACAAGGG GGCACTGTGT GACACCAACC CCCTAAATGG GCAATATATT 
TGCACCTGCC CACAAGGCTA CAAAGGGGCT GACTGCACAG AAGATGTGGA TGAATGTTCC 
ATGGCCAATA GCAATCCTTG TGAGCATGCA GGAAAATGTG TGAACACGGA TGGCGCCTTC 
CACTGTGAGT GTCTGAAGGG TTATGCAGGA CCTCGTTGTG AGATGGACAT CAATGAGTGC 
CATTCAGACC CCTGCCAGAA TGATGCTACC TGTCTGGATA AGATTGGAGG CTTCACATGT 
CTGTGCATGC CAGGTTTCAA AGGTGTGCAT TGTGAATTAG AAATAAATGA ATGTCAGAGC 
AACCCTTGTG TGAACAATGG GCAGTGTGTG GATAAAGTCA ATCGTTTCCA GTGCCTGTGT 
CCTCCTGGTT TCACTGGGCC AGTTTGCCAG ATTGATATTG ATGACTGTTC CAGTACTCCG 
TGTCTGAATG GGGCAAAGTG TATCGATCAC CCGAATGGCT ATGAATGCCA GTGTGCCACA 
GGTTTCACTG GTGTGTTGTG TGAGGAGAAC ATTGACAACT GTGACCCCGA TCCTTGCCAC 
CATGGTCAGT GTCAGGATGG TATTGATTCC TACACCTGCA TCTGCAATCC CGGGTACATG 
GGCGCCATCT GCAGTGACCA GATTGATGAA TGTTACAGCA GCCCTTGCCT GAACGATGGT 
CGCTGCATTG ACCTGGTCAA TGGCTACCAG TGCAACTGCC AGCCGGGCAC GTCAGGGGTT 
AATTGTGAAA TTAATTTTGA TGACTGTGCA AGTAACCCTT GTATCCATGG AAACTGTATG 
GATGGCATTA ATCGCTACAG TTGTGTCTGC TCACCAGGAT TCACAGGGCA GAGATGTAAC 
ATTGACATTG ATGAGTGTGC CTCCAATCCC TGTCGCAAGG GTGCAACATG TATCAACGGT 
GTGAATGGTT TCCGCTGTAT ATGCCCCGAG GGACCCCATC ACCCCAGCTG CTACTCACAG 
GTGAACGAAT GCCTGAGCAA TCCCTGCATC CATGGAAACT GTACTGGAGG TCTCAGTGGA 
TATAAGTGTC TCTGTGATGC AGGCTGGGTT GGCATCAACT GTGAAGTGGA CAAAAATGAA 
TGCCTTTCGA ATCCATGCCA GAATGGAGGA ACTTGTGACA ATCTGGTGAA TGGATACAGG 
TGTACTTGCA AGAAGGGCTT TAAAGGCTAT AACTGCCAGG TGAATATTGA TGAATGTGCC 
TCAAATCCAT GCCTGAACCA AGGAACCTGC TTTGATGACA TAAGTGGCTA CACTTGCCAC 
TGTGTGCTGC CATACACAGG CAAGAATTGT CAGACAGTAT TGGCTCCCTG TTCCCCAAAC 
CCTTGTGAGA ATGCTGCTGT TTGCAAAGAG TCACCAAATT TTGAGAGTTA TACTTGCTTG 
TGTGCTCCTG GCTGGCAAGG TCAGCGGTGT ACCATTGACA TTGACGAGTG TATCTCCAAG 
CCCTGCATGA ACCATGGTCT CTGCCATAAC ACCCAGGGCA GCTACATGTG TGAATGTCCA 
CCAGGCTTCA GTGGTATGGA CTGTGAGGAG GACATTGATG ACTGCCTTGC CAATCCTTGC 
CAGAATGGAG GTTCCTGTGT GGATGGAGTG AATACTTTCT CCTGCCTCTG CCTTCCGGGT 
TTCACTGGGG ATAAGTGCCA GACAGACATG AATGAGTGTC TGAGTGAACC CTGTAAGAAT 
GGAGGGACCT GCTCTGACTA CGTCAACAGT TACACTTGCA AGTGCCAGGC AGGATTTGAT 
GGAGTCCATT GTGAGAACAA CATCAATGAG TGCACTGAGA GCTCCTGTTT CAATGGTGGC 
ACATGTGTTG ATGGGATTAA CTCCTTCTCT TGCTTGTGCC CTGTGGGTTT CACTGGATCC 
TTCTGCCTCC ATGAGATCAA TGAATGCAGC TCTCATCCAT GCCTCAATGA GGGAACGTGT 
GTTGATGGCC TGGGTACCTA CCGCTGCAGC TGCCCCCTGG GCTACACTGG GAAAAACTGT 
CAGACCCTGG TGAACCTCTG CAGTCGGTCT CCATGTAAAA ACAAAGGTAC TTGCGTTCAG 
AAAAAAGCAG AGTCCCAGTG CCTATGTCCA TCTGGATGGG CTGGTGCCTA TTGTGACGTG 
CCCAATGTCT CTTGTGACAT AGCAGCCTCC AGGAGAGGTG TGCTTGTTGA ACACTTGTGC 
CAGCACTCAG GTGTCTGCAT CAATGCTGGC AACACGCATT ACTGTCAGTG CCCCCTGGGC 
TATACTGGGA GCTACTGTGA GGAGCAACTC GATGAGTGTG CGTCCAACCC CTGCCAGCAC 
GGGGCAACAT GCAGTGACTT CATTGGTGGA TACAGATGTG AGTGTGTCCC AGGCTATCAG 
GGTGTCAACT GTGAGTATGA AGTGGATGAG TGCCAGAATC AGCCCTGCCA GAATGGAGGC 
ACCTGTATTG ACCTTGTGAA CCATTTCAAG TGCTCTTGCC CACCAGGCAC TCGGGGCCTA 
CTCTGTGAAG AGAACATTGA TGACTGTGCC CGGGGTCCCC ATTGCCTTAA TGGTGGTCAG 
TGCATGGATA GGATTGGAGG CTACAGTTGT CGCTGCTTGC CTGGCTTTGC TGGGGAGCGT 
TGTGAGGGAG ACATCAACGA GTGCCTCTCC AACCCCTGCA GCTCTGAGGG CAGCCTGGAC 
TGTATACAGC TCACCAATGA CTACCTGTGT GTTTGCCGTA GTGCCTTTAC TGGCCGGCAC 
TGTGAAACCT TCGTCGATGT GTGTCCCCAG ATGCCCTGCC TGAATGGAGG GACTTGTGCT 
GTGGCCAGTA ACATGCCTGA TGGTTTCATT TGCCGTTGTC CCCCGGGATT TTCTGGGGCA 
AGGTGCCAGA GCAGCTGTGG ACAAGTGAAA TGTAGGAAAG GGGAGCAGTG TGTGCACACC 
GCCTCTGGAC CCCGCTGCTT CTGCCCCAGT CCCCGGGACT GCGAGTCAGG CTGTGCCAGT 
AGCCCCTGCC AGCACGGGGG CAGCTGCCAC CCTCAGCGCC AGCCTCCTTA TTACTCCTGC 
CAGTGTGCCC CACCATTCTC GGGTAGCCGC TGTGAACTCT ACACGGCACC CCCCAGCACC 
CCTCCTGCCA CCTGTCTGAG CCAGTATTGT GCCGACAAAG CTCGGGATGG CGTCTGTGAT 
GAGGCCTGCA ACAGCCATGC CTGCCAGTGG GATGGGGGTG ACTGTTCTCT CACCATGGAG 
AACCCCTGGG CCAACTGCTC CTCCCCACTT CCCTGCTGGG ATTATATCAA CAACCAGTGT 
GATGAGCTGT GCAACACGGT CGAGTGCCTG TTTGACAACT TTGAATGCCA GGGGAACAGC 
AAGACATGCA AGTATGACAA ATACTGTGCA GACCACTTCA AAGACAACCA CTGTGACCAG 
GGGTGCAACA GTGAGGAGTG TGGTTGGGAT GGGCTGGACT GTGCTGCTGA CCAACCTGAG 
AACCTGGCAG AAGGTACCCT GGTTATTGTG GTATTGATGC CACCTGAACA ACTGCTCCAG 
GATGCTCGCA GCTTCTTGCG GGCACTGGGT ACCCTGCTGC ACACCAACCT GCGCATTAAG 
CGGGACTCCC AGGGGGAACT CATGGTGTAC CCCTATTATG GTGAGAAGTC AGCTGCTATG 
AAGAAACAGA GGATGACACG CAGGTCCCTT CCTGGTGAAC AAGAACAGGA GGTGGCTGGC 
TCTAAAGTCT TTCTGGAAAT TGACAACCGC CAGTGTGTTC AAGACTCAGA CCACTGCTTC 
AAGAACACGG ATGCAGCAGC AGCTCTCCTG GCCTCTCACG CCATACAGGG GACCCTGTCA 
TACCCTCTTG TGTCTGTCGT CAGTGAATCC CTGACTCCAG AACGCACTCA GCTCCTCTAT 
CTCCTTGCTG TTGCTGTTGT CATCATTCTG TTTATTATTC TGCTGGGGGT AATCATGGCA 
AAACGAAAGC GTAAGCATGG CTCTCTCTGG CTGCCTGAAG GTTTCACTCT TCGCCGAGAT 
GCAAGCAATC ACAAGCGTCG TGAGCCAGTG GGACAGGATG CTGTGGGGCT AAAAAATCTC 
TCAGTGCAAG TCTCAGAAGC TAACCTAATT GGTTCTGGAA CAAGTGAACA CTGGGTCGAT 
GATGAAGGGC CCCAGCCAAA GAAAGTAAAG GCTGAAGATG AGGCCTTACT CTCAGAAGAA 
GATGACCCCA TTGATCGACG GCCATGGACA CAGCAGCACC TTGAAGCCGC AGACATCCGT 
AGGACACCAT CGCTGGCTCT CACCCCTCCT CAGGCAGAGC AGGAGGTGGA TGTGTTAGAT 
GTGAATGTCC GTGGCCCAGA TGGCTGCACC CCATTGATGT TGGCTTCTCT CCGAGGAGGC 
AGCTCAGATT TGAGTGATGA AGATGAAGAT GCAGAGGACT CTTCTGCTAA CATCATCACA 
GACTTGGTCT ACCAGGGTGC CAGCCTCCAG GCCCAGACAG ACCGGACTGG TGAGATGGCC 
CTGCACCTTG CAGCCCGCTA CTCACGGGCT GATGCTGCCA AGCGTCTCCT GGATGCAGGT 
GCAGATGCCA ATGCCCAGGA CAACATGGGC CGCTGCCCAC TCCATGCTGC AGTGGCAGCT 
GATGCCCAAG GTGTCTTCCA GATTCTGATT CGCAACCGAG TAACTGATCT AGATGCCAGG 
ATGAATGATG GTACTACACC CCTGATCCTG GCTGCCCGCC TGGCTGTGGA GGGAATGGTG 
GCAGAACTGA TCAACTGCCA AGCGGATGTG AATGCAGTGG ATGACCATGG AAAATCTGCT 
CTTCACTGGG CAGCTGCTGT CAATAATGTG GAGGCAACTC TTTTGTTGTT GAAAAATGGA 
GCCAACCGAG ACATGCAGGA CAACAAGGAA GAGACACCTC TGTTTCTTGC TGCCCGGGAG 
GGGAGCTATG AAGCAGCCAA GATCCTGTTA GACCATTTTG CCAATCGAGA CATCACAGAC 
CATATGGATC GTCTTCCCCG GGATGTGGCT CGGGATCGCA TGCACCATGA CATTGTGCGC 
CTTCTGGATG AATACAATGT GACCCCAAGC CCTCCAGGCA CCGTGTTGAC TTCTGCTCTC 
TCACCTGTCA TCTGTGGGCC CAACAGATCT TTCCTCAGCC TGAAGCACAC CCCAATGGGC 
AAGAAGTCTA GACGGCCCAG TGCCAAGAGT ACCATGCCTA CTAGCCTCCC TAACCTTGCC 
AAGGAGGCAA AGGATGCCAA GGGTAGTAGG AGGAAGAAGT CTCTGAGTGA GAAGGTCCAA 
CTGTCTGAGA GTTCAGTAAC TTTATCCCCT GTTGATTCCC TAGAATCTCC TCACACGTAT 
GTTTCCGACA CCACATCCTC TCCAATGATT ACATCCCCTG GGATCTTACA GGCCTCACCC 
AACCCTATGT TGGCCACTGC CGCCCCTCCT GCCCCAGTCC ATGCCCAGCA TGCACTATCT 
TTTTCTAACC TTCATGAAAT GCAGCCTTTG GCACATGGGG CCAGCACTGT GCTTCCCTCA 
GTGAGCCAGT TGCTATCCCA CCACCACATT GTGTCTCCAG GCAGTGGCAG TGCTGGAAGC 
TTGAGTAGGC TCCATCCAGT CCCAGTCCCA GCAGATTGGA TGAACCGCAT GGAGGTGAAT 
GAGACCCAGT ACAATGAGAT GTTTGGTATG GTCCTGGCTC CAGCTGAGGG CACCCATCCT 
GGCATAGCTC CCCAGAGCAG GCCACCTGAA GGGAAGCACA TAACCACCCC TCGGGAGCCC 
TTGCCCCCCA TTGTGACTTT CCAGCTCATC CCTAAAGGCA GTATTGCCCA ACCAGCGGGG 
GCTCCCCAGC CTCAGTCCAC CTGCCCTCCA GCTGTTGCGG GCCCCCTGCC CACCATGTAC 
CAGATTCCAG AAATGGCCCG TTTGCCCAGT GTGGCTTTCC CCACTGCCAT GATGCCCCAG 
CAGGACGGGC AGGTAGCTCA GACCATTCTC CCAGCCTATC ATCCTTTCCC AGCCTCTGTG 
GGCAAGTACC CCACACCCCC TTCACAGCAC AGTTATGCTT CCTCAAATGC TGCTGAGCGA 
ACACCCAGTC ACAGTGGTCA CCTCCAGGGT GAGCATCCCT ACCTGACACC ATCCCCAGAG 
TCTCCTGACC AGTGGTCAAG TTCATCACCC CACTCTGCTT CTGACTGGTC AGATGTGACC 
ACCAGCCCTA CCCCTGGGGG TGCTGGAGGA GGTCAGCGGG GACCTGGGAC ACACATGTCT 
GAGCCACCAC ACAACAACAT GCAGGTTTAT GCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024210692.1
CDS	168..7583
Translation	MKGHQRSEGPAAECQQGHSEPQEPALQCRDGYEPCVNEGMCVTYHNGTGYCKCPEGFLGEYCQHRDPCEKNRCQNGGTCVAQAMLGKATCRCASGFTGEDCQYSTSHPCFVSRPCLNGGTCHMLSRDTYECTCQVGFTGKECQWTDACLSHPCANGSTCTTVANQFSCKCLTGFTGQKCETDVNECDIPGHCQHGGTCLNLPGSYQCQCPQGFTGQYCDSLYVPCAPSPCVNGGTCRQTGDFTFECSCLPGFEGSTCERNIDDCPNHRCQNGGVCVDGVNTYNCRCPPQWTGQFCTEDVDECLLQPNACQNGGTCANRNGGYGCVCVNGWSGDDCSENIDDCAFASCTPGSTCIDRVASFSCMCPEGKAGLLCHLDDACISNPCHKGALCDTNPLNGQYICTCPQGYKGADCTEDVDECSMANSNPCEHAGKCVNTDGAFHCECLKGYAGPRCEMDINECHSDPCQNDATCLDKIGGFTCLCMPGFKGVHCELEINECQSNPCVNNGQCVDKVNRFQCLCPPGFTGPVCQIDIDDCSSTPCLNGAKCIDHPNGYECQCATGFTGVLCEENIDNCDPDPCHHGQCQDGIDSYTCICNPGYMGAICSDQIDECYSSPCLNDGRCIDLVNGYQCNCQPGTSGVNCEINFDDCASNPCIHGNCMDGINRYSCVCSPGFTGQRCNIDIDECASNPCRKGATCINGVNGFRCICPEGPHHPSCYSQVNECLSNPCIHGNCTGGLSGYKCLCDAGWVGINCEVDKNECLSNPCQNGGTCDNLVNGYRCTCKKGFKGYNCQVNIDECASNPCLNQGTCFDDISGYTCHCVLPYTGKNCQTVLAPCSPNPCENAAVCKESPNFESYTCLCAPGWQGQRCTIDIDECISKPCMNHGLCHNTQGSYMCECPPGFSGMDCEEDIDDCLANPCQNGGSCVDGVNTFSCLCLPGFTGDKCQTDMNECLSEPCKNGGTCSDYVNSYTCKCQAGFDGVHCENNINECTESSCFNGGTCVDGINSFSCLCPVGFTGSFCLHEINECSSHPCLNEGTCVDGLGTYRCSCPLGYTGKNCQTLVNLCSRSPCKNKGTCVQKKAESQCLCPSGWAGAYCDVPNVSCDIAASRRGVLVEHLCQHSGVCINAGNTHYCQCPLGYTGSYCEEQLDECASNPCQHGATCSDFIGGYRCECVPGYQGVNCEYEVDECQNQPCQNGGTCIDLVNHFKCSCPPGTRGLLCEENIDDCARGPHCLNGGQCMDRIGGYSCRCLPGFAGERCEGDINECLSNPCSSEGSLDCIQLTNDYLCVCRSAFTGRHCETFVDVCPQMPCLNGGTCAVASNMPDGFICRCPPGFSGARCQSSCGQVKCRKGEQCVHTASGPRCFCPSPRDCESGCASSPCQHGGSCHPQRQPPYYSCQCAPPFSGSRCELYTAPPSTPPATCLSQYCADKARDGVCDEACNSHACQWDGGDCSLTMENPWANCSSPLPCWDYINNQCDELCNTVECLFDNFECQGNSKTCKYDKYCADHFKDNHCDQGCNSEECGWDGLDCAADQPENLAEGTLVIVVLMPPEQLLQDARSFLRALGTLLHTNLRIKRDSQGELMVYPYYGEKSAAMKKQRMTRRSLPGEQEQEVAGSKVFLEIDNRQCVQDSDHCFKNTDAAAALLASHAIQGTLSYPLVSVVSESLTPERTQLLYLLAVAVVIILFIILLGVIMAKRKRKHGSLWLPEGFTLRRDASNHKRREPVGQDAVGLKNLSVQVSEANLIGSGTSEHWVDDEGPQPKKVKAEDEALLSEEDDPIDRRPWTQQHLEAADIRRTPSLALTPPQAEQEVDVLDVNVRGPDGCTPLMLASLRGGSSDLSDEDEDAEDSSANIITDLVYQGASLQAQTDRTGEMALHLAARYSRADAAKRLLDAGADANAQDNMGRCPLHAAVAADAQGVFQILIRNRVTDLDARMNDGTTPLILAARLAVEGMVAELINCQADVNAVDDHGKSALHWAAAVNNVEATLLLLKNGANRDMQDNKEETPLFLAAREGSYEAAKILLDHFANRDITDHMDRLPRDVARDRMHHDIVRLLDEYNVTPSPPGTVLTSALSPVICGPNRSFLSLKHTPMGKKSRRPSAKSTMPTSLPNLAKEAKDAKGSRRKKSLSEKVQLSESSVTLSPVDSLESPHTYVSDTTSSPMITSPGILQASPNPMLATAAPPAPVHAQHALSFSNLHEMQPLAHGASTVLPSVSQLLSHHHIVSPGSGSAGSLSRLHPVPVPADWMNRMEVNETQYNEMFGMVLAPAEGTHPGIAPQSRPPEGKHITTPREPLPPIVTFQLIPKGSIAQPAGAPQPQSTCPPAVAGPLPTMYQIPEMARLPSVAFPTAMMPQQDGQVAQTILPAYHPFPASVGKYPTPPSQHSYASSNAAERTPSHSGHLQGEHPYLTPSPESPDQWSSSSPHSASDWSDVTTSPTPGGAGGGQRGPGTHMSEPPHNNMQVYA

Target ORF information:

RefSeq Version	XM_024354924.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes notch 2 (NOTCH2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024354924.1

ORF Insert Sequence:

ATGAAAGGAC ATCAAAGATC TGAAGGCCCA GCTGCAGAAT GCCAACAAGG TCATTCAGAA 
CCTCAAGAGC CAGCATTGCA GTGTCGAGAT GGCTATGAAC CCTGTGTAAA TGAAGGAATG 
TGTGTTACCT ACCACAATGG CACAGGATAC TGCAAATGTC CAGAAGGCTT CTTGGGGGAA 
TATTGTCAAC ATCGAGACCC CTGTGAGAAG AACCGCTGCC AGAATGGTGG GACTTGTGTG 
GCCCAGGCCA TGCTGGGGAA AGCCACGTGC CGATGTGCCT CAGGGTTTAC AGGAGAGGAC 
TGCCAGTACT CGACATCTCA TCCATGCTTT GTGTCTCGAC CCTGCCTGAA TGGCGGCACA 
TGCCATATGC TCAGCCGGGA TACCTATGAG TGCACCTGTC AAGTCGGGTT TACAGGTAAG 
GAGTGCCAAT GGACCGATGC CTGCCTGTCT CATCCCTGTG CAAATGGAAG TACCTGTACC 
ACTGTGGCCA ACCAGTTCTC CTGCAAATGC CTCACAGGCT TCACAGGGCA GAAGTGTGAG 
ACTGATGTCA ATGAGTGTGA CATTCCAGGA CACTGCCAGC ATGGTGGCAC CTGCCTCAAC 
CTGCCTGGTT CCTACCAGTG CCAGTGCCCT CAGGGCTTCA CAGGCCAGTA CTGTGACAGC 
CTGTATGTGC CCTGTGCACC CTCGCCTTGT GTCAATGGAG GCACCTGTCG GCAGACTGGT 
GACTTCACTT TTGAGTGCAG CTGCCTTCCA GGTTTTGAAG GGAGCACCTG TGAGAGGAAT 
ATTGATGACT GCCCTAACCA CAGGTGTCAG AATGGAGGGG TTTGTGTGGA TGGGGTCAAC 
ACTTACAACT GCCGCTGTCC CCCACAATGG ACAGGACAGT TCTGCACAGA GGATGTGGAT 
GAATGCCTGC TGCAGCCCAA TGCCTGTCAA AATGGGGGCA CCTGTGCCAA CCGCAATGGA 
GGCTATGGCT GTGTATGTGT CAACGGCTGG AGTGGAGATG ACTGCAGTGA GAACATTGAT 
GATTGTGCCT TCGCCTCCTG TACTCCAGGC TCCACCTGCA TCGACCGTGT GGCCTCCTTC 
TCTTGCATGT GCCCAGAGGG GAAGGCAGGT CTCCTGTGTC ATCTGGATGA TGCATGCATC 
AGCAATCCTT GCCACAAGGG GGCACTGTGT GACACCAACC CCCTAAATGG GCAATATATT 
TGCACCTGCC CACAAGGCTA CAAAGGGGCT GACTGCACAG AAGATGTGGA TGAATGTTCC 
ATGGCCAATA GCAATCCTTG TGAGCATGCA GGAAAATGTG TGAACACGGA TGGCGCCTTC 
CACTGTGAGT GTCTGAAGGG TTATGCAGGA CCTCGTTGTG AGATGGACAT CAATGAGTGC 
CATTCAGACC CCTGCCAGAA TGATGCTACC TGTCTGGATA AGATTGGAGG CTTCACATGT 
CTGTGCATGC CAGGTTTCAA AGGTGTGCAT TGTGAATTAG AAATAAATGA ATGTCAGAGC 
AACCCTTGTG TGAACAATGG GCAGTGTGTG GATAAAGTCA ATCGTTTCCA GTGCCTGTGT 
CCTCCTGGTT TCACTGGGCC AGTTTGCCAG ATTGATATTG ATGACTGTTC CAGTACTCCG 
TGTCTGAATG GGGCAAAGTG TATCGATCAC CCGAATGGCT ATGAATGCCA GTGTGCCACA 
GGTTTCACTG GTGTGTTGTG TGAGGAGAAC ATTGACAACT GTGACCCCGA TCCTTGCCAC 
CATGGTCAGT GTCAGGATGG TATTGATTCC TACACCTGCA TCTGCAATCC CGGGTACATG 
GGCGCCATCT GCAGTGACCA GATTGATGAA TGTTACAGCA GCCCTTGCCT GAACGATGGT 
CGCTGCATTG ACCTGGTCAA TGGCTACCAG TGCAACTGCC AGCCGGGCAC GTCAGGGGTT 
AATTGTGAAA TTAATTTTGA TGACTGTGCA AGTAACCCTT GTATCCATGG AAACTGTATG 
GATGGCATTA ATCGCTACAG TTGTGTCTGC TCACCAGGAT TCACAGGGCA GAGATGTAAC 
ATTGACATTG ATGAGTGTGC CTCCAATCCC TGTCGCAAGG GTGCAACATG TATCAACGGT 
GTGAATGGTT TCCGCTGTAT ATGCCCCGAG GGACCCCATC ACCCCAGCTG CTACTCACAG 
GTGAACGAAT GCCTGAGCAA TCCCTGCATC CATGGAAACT GTACTGGAGG TCTCAGTGGA 
TATAAGTGTC TCTGTGATGC AGGCTGGGTT GGCATCAACT GTGAAGTGGA CAAAAATGAA 
TGCCTTTCGA ATCCATGCCA GAATGGAGGA ACTTGTGACA ATCTGGTGAA TGGATACAGG 
TGTACTTGCA AGAAGGGCTT TAAAGGCTAT AACTGCCAGG TGAATATTGA TGAATGTGCC 
TCAAATCCAT GCCTGAACCA AGGAACCTGC TTTGATGACA TAAGTGGCTA CACTTGCCAC 
TGTGTGCTGC CATACACAGG CAAGAATTGT CAGACAGTAT TGGCTCCCTG TTCCCCAAAC 
CCTTGTGAGA ATGCTGCTGT TTGCAAAGAG TCACCAAATT TTGAGAGTTA TACTTGCTTG 
TGTGCTCCTG GCTGGCAAGG TCAGCGGTGT ACCATTGACA TTGACGAGTG TATCTCCAAG 
CCCTGCATGA ACCATGGTCT CTGCCATAAC ACCCAGGGCA GCTACATGTG TGAATGTCCA 
CCAGGCTTCA GTGGTATGGA CTGTGAGGAG GACATTGATG ACTGCCTTGC CAATCCTTGC 
CAGAATGGAG GTTCCTGTGT GGATGGAGTG AATACTTTCT CCTGCCTCTG CCTTCCGGGT 
TTCACTGGGG ATAAGTGCCA GACAGACATG AATGAGTGTC TGAGTGAACC CTGTAAGAAT 
GGAGGGACCT GCTCTGACTA CGTCAACAGT TACACTTGCA AGTGCCAGGC AGGATTTGAT 
GGAGTCCATT GTGAGAACAA CATCAATGAG TGCACTGAGA GCTCCTGTTT CAATGGTGGC 
ACATGTGTTG ATGGGATTAA CTCCTTCTCT TGCTTGTGCC CTGTGGGTTT CACTGGATCC 
TTCTGCCTCC ATGAGATCAA TGAATGCAGC TCTCATCCAT GCCTCAATGA GGGAACGTGT 
GTTGATGGCC TGGGTACCTA CCGCTGCAGC TGCCCCCTGG GCTACACTGG GAAAAACTGT 
CAGACCCTGG TGAACCTCTG CAGTCGGTCT CCATGTAAAA ACAAAGGTAC TTGCGTTCAG 
AAAAAAGCAG AGTCCCAGTG CCTATGTCCA TCTGGATGGG CTGGTGCCTA TTGTGACGTG 
CCCAATGTCT CTTGTGACAT AGCAGCCTCC AGGAGAGGTG TGCTTGTTGA ACACTTGTGC 
CAGCACTCAG GTGTCTGCAT CAATGCTGGC AACACGCATT ACTGTCAGTG CCCCCTGGGC 
TATACTGGGA GCTACTGTGA GGAGCAACTC GATGAGTGTG CGTCCAACCC CTGCCAGCAC 
GGGGCAACAT GCAGTGACTT CATTGGTGGA TACAGATGTG AGTGTGTCCC AGGCTATCAG 
GGTGTCAACT GTGAGTATGA AGTGGATGAG TGCCAGAATC AGCCCTGCCA GAATGGAGGC 
ACCTGTATTG ACCTTGTGAA CCATTTCAAG TGCTCTTGCC CACCAGGCAC TCGGGGCCTA 
CTCTGTGAAG AGAACATTGA TGACTGTGCC CGGGGTCCCC ATTGCCTTAA TGGTGGTCAG 
TGCATGGATA GGATTGGAGG CTACAGTTGT CGCTGCTTGC CTGGCTTTGC TGGGGAGCGT 
TGTGAGGGAG ACATCAACGA GTGCCTCTCC AACCCCTGCA GCTCTGAGGG CAGCCTGGAC 
TGTATACAGC TCACCAATGA CTACCTGTGT GTTTGCCGTA GTGCCTTTAC TGGCCGGCAC 
TGTGAAACCT TCGTCGATGT GTGTCCCCAG ATGCCCTGCC TGAATGGAGG GACTTGTGCT 
GTGGCCAGTA ACATGCCTGA TGGTTTCATT TGCCGTTGTC CCCCGGGATT TTCTGGGGCA 
AGGTGCCAGA GCAGCTGTGG ACAAGTGAAA TGTAGGAAAG GGGAGCAGTG TGTGCACACC 
GCCTCTGGAC CCCGCTGCTT CTGCCCCAGT CCCCGGGACT GCGAGTCAGG CTGTGCCAGT 
AGCCCCTGCC AGCACGGGGG CAGCTGCCAC CCTCAGCGCC AGCCTCCTTA TTACTCCTGC 
CAGTGTGCCC CACCATTCTC GGGTAGCCGC TGTGAACTCT ACACGGCACC CCCCAGCACC 
CCTCCTGCCA CCTGTCTGAG CCAGTATTGT GCCGACAAAG CTCGGGATGG CGTCTGTGAT 
GAGGCCTGCA ACAGCCATGC CTGCCAGTGG GATGGGGGTG ACTGTTCTCT CACCATGGAG 
AACCCCTGGG CCAACTGCTC CTCCCCACTT CCCTGCTGGG ATTATATCAA CAACCAGTGT 
GATGAGCTGT GCAACACGGT CGAGTGCCTG TTTGACAACT TTGAATGCCA GGGGAACAGC 
AAGACATGCA AGTATGACAA ATACTGTGCA GACCACTTCA AAGACAACCA CTGTGACCAG 
GGGTGCAACA GTGAGGAGTG TGGTTGGGAT GGGCTGGACT GTGCTGCTGA CCAACCTGAG 
AACCTGGCAG AAGGTACCCT GGTTATTGTG GTATTGATGC CACCTGAACA ACTGCTCCAG 
GATGCTCGCA GCTTCTTGCG GGCACTGGGT ACCCTGCTGC ACACCAACCT GCGCATTAAG 
CGGGACTCCC AGGGGGAACT CATGGTGTAC CCCTATTATG GTGAGAAGTC AGCTGCTATG 
AAGAAACAGA GGATGACACG CAGGTCCCTT CCTGGTGAAC AAGAACAGGA GGTGGCTGGC 
TCTAAAGTCT TTCTGGAAAT TGACAACCGC CAGTGTGTTC AAGACTCAGA CCACTGCTTC 
AAGAACACGG ATGCAGCAGC AGCTCTCCTG GCCTCTCACG CCATACAGGG GACCCTGTCA 
TACCCTCTTG TGTCTGTCGT CAGTGAATCC CTGACTCCAG AACGCACTCA GCTCCTCTAT 
CTCCTTGCTG TTGCTGTTGT CATCATTCTG TTTATTATTC TGCTGGGGGT AATCATGGCA 
AAACGAAAGC GTAAGCATGG CTCTCTCTGG CTGCCTGAAG GTTTCACTCT TCGCCGAGAT 
GCAAGCAATC ACAAGCGTCG TGAGCCAGTG GGACAGGATG CTGTGGGGCT AAAAAATCTC 
TCAGTGCAAG TCTCAGAAGC TAACCTAATT GGTTCTGGAA CAAGTGAACA CTGGGTCGAT 
GATGAAGGGC CCCAGCCAAA GAAAGTAAAG GCTGAAGATG AGGCCTTACT CTCAGAAGAA 
GATGACCCCA TTGATCGACG GCCATGGACA CAGCAGCACC TTGAAGCCGC AGACATCCGT 
AGGACACCAT CGCTGGCTCT CACCCCTCCT CAGGCAGAGC AGGAGGTGGA TGTGTTAGAT 
GTGAATGTCC GTGGCCCAGA TGGCTGCACC CCATTGATGT TGGCTTCTCT CCGAGGAGGC 
AGCTCAGATT TGAGTGATGA AGATGAAGAT GCAGAGGACT CTTCTGCTAA CATCATCACA 
GACTTGGTCT ACCAGGGTGC CAGCCTCCAG GCCCAGACAG ACCGGACTGG TGAGATGGCC 
CTGCACCTTG CAGCCCGCTA CTCACGGGCT GATGCTGCCA AGCGTCTCCT GGATGCAGGT 
GCAGATGCCA ATGCCCAGGA CAACATGGGC CGCTGCCCAC TCCATGCTGC AGTGGCAGCT 
GATGCCCAAG GTGTCTTCCA GATTCTGATT CGCAACCGAG TAACTGATCT AGATGCCAGG 
ATGAATGATG GTACTACACC CCTGATCCTG GCTGCCCGCC TGGCTGTGGA GGGAATGGTG 
GCAGAACTGA TCAACTGCCA AGCGGATGTG AATGCAGTGG ATGACCATGG AAAATCTGCT 
CTTCACTGGG CAGCTGCTGT CAATAATGTG GAGGCAACTC TTTTGTTGTT GAAAAATGGA 
GCCAACCGAG ACATGCAGGA CAACAAGGAA GAGACACCTC TGTTTCTTGC TGCCCGGGAG 
GGGAGCTATG AAGCAGCCAA GATCCTGTTA GACCATTTTG CCAATCGAGA CATCACAGAC 
CATATGGATC GTCTTCCCCG GGATGTGGCT CGGGATCGCA TGCACCATGA CATTGTGCGC 
CTTCTGGATG AATACAATGT GACCCCAAGC CCTCCAGGCA CCGTGTTGAC TTCTGCTCTC 
TCACCTGTCA TCTGTGGGCC CAACAGATCT TTCCTCAGCC TGAAGCACAC CCCAATGGGC 
AAGAAGTCTA GACGGCCCAG TGCCAAGAGT ACCATGCCTA CTAGCCTCCC TAACCTTGCC 
AAGGAGGCAA AGGATGCCAA GGGTAGTAGG AGGAAGAAGT CTCTGAGTGA GAAGGTCCAA 
CTGTCTGAGA GTTCAGTAAC TTTATCCCCT GTTGATTCCC TAGAATCTCC TCACACGTAT 
GTTTCCGACA CCACATCCTC TCCAATGATT ACATCCCCTG GGATCTTACA GGCCTCACCC 
AACCCTATGT TGGCCACTGC CGCCCCTCCT GCCCCAGTCC ATGCCCAGCA TGCACTATCT 
TTTTCTAACC TTCATGAAAT GCAGCCTTTG GCACATGGGG CCAGCACTGT GCTTCCCTCA 
GTGAGCCAGT TGCTATCCCA CCACCACATT GTGTCTCCAG GCAGTGGCAG TGCTGGAAGC 
TTGAGTAGGC TCCATCCAGT CCCAGTCCCA GCAGATTGGA TGAACCGCAT GGAGGTGAAT 
GAGACCCAGT ACAATGAGAT GTTTGGTATG GTCCTGGCTC CAGCTGAGGG CACCCATCCT 
GGCATAGCTC CCCAGAGCAG GCCACCTGAA GGGAAGCACA TAACCACCCC TCGGGAGCCC 
TTGCCCCCCA TTGTGACTTT CCAGCTCATC CCTAAAGGCA GTATTGCCCA ACCAGCGGGG 
GCTCCCCAGC CTCAGTCCAC CTGCCCTCCA GCTGTTGCGG GCCCCCTGCC CACCATGTAC 
CAGATTCCAG AAATGGCCCG TTTGCCCAGT GTGGCTTTCC CCACTGCCAT GATGCCCCAG 
CAGGACGGGC AGGTAGCTCA GACCATTCTC CCAGCCTATC ATCCTTTCCC AGCCTCTGTG 
GGCAAGTACC CCACACCCCC TTCACAGCAC AGTTATGCTT CCTCAAATGC TGCTGAGCGA 
ACACCCAGTC ACAGTGGTCA CCTCCAGGGT GAGCATCCCT ACCTGACACC ATCCCCAGAG 
TCTCCTGACC AGTGGTCAAG TTCATCACCC CACTCTGCTT CTGACTGGTC AGATGTGACC 
ACCAGCCCTA CCCCTGGGGG TGCTGGAGGA GGTCAGCGGG GACCTGGGAC ACACATGTCT 
GAGCCACCAC ACAACAACAT GCAGGTTTAT GCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NOTCH2 ( XM_024354928.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_024210696.1 Pan troglodytes notch 2 (NOTCH2), transcript variant X3, mRNA.

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CloneID	OPc92101
Clone ID Related Accession (Same CDS sequence)	XM_024354928.1 , XM_024354927.1
Accession Version	XM_024354928.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7299bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	neurogenic locus notch homolog protein 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036879.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTGTTA CCTACCACAA TGGCACAGGA TACTGCAAAT GTCCAGAAGG CTTCTTGGGG 
GAATATTGTC AACATCGAGA CCCCTGTGAG AAGAACCGCT GCCAGAATGG TGGGACTTGT 
GTGGCCCAGG CCATGCTGGG GAAAGCCACG TGCCGATGTG CCTCAGGGTT TACAGGAGAG 
GACTGCCAGT ACTCGACATC TCATCCATGC TTTGTGTCTC GACCCTGCCT GAATGGCGGC 
ACATGCCATA TGCTCAGCCG GGATACCTAT GAGTGCACCT GTCAAGTCGG GTTTACAGGT 
AAGGAGTGCC AATGGACCGA TGCCTGCCTG TCTCATCCCT GTGCAAATGG AAGTACCTGT 
ACCACTGTGG CCAACCAGTT CTCCTGCAAA TGCCTCACAG GCTTCACAGG GCAGAAGTGT 
GAGACTGATG TCAATGAGTG TGACATTCCA GGACACTGCC AGCATGGTGG CACCTGCCTC 
AACCTGCCTG GTTCCTACCA GTGCCAGTGC CCTCAGGGCT TCACAGGCCA GTACTGTGAC 
AGCCTGTATG TGCCCTGTGC ACCCTCGCCT TGTGTCAATG GAGGCACCTG TCGGCAGACT 
GGTGACTTCA CTTTTGAGTG CAGCTGCCTT CCAGGTTTTG AAGGGAGCAC CTGTGAGAGG 
AATATTGATG ACTGCCCTAA CCACAGGTGT CAGAATGGAG GGGTTTGTGT GGATGGGGTC 
AACACTTACA ACTGCCGCTG TCCCCCACAA TGGACAGGAC AGTTCTGCAC AGAGGATGTG 
GATGAATGCC TGCTGCAGCC CAATGCCTGT CAAAATGGGG GCACCTGTGC CAACCGCAAT 
GGAGGCTATG GCTGTGTATG TGTCAACGGC TGGAGTGGAG ATGACTGCAG TGAGAACATT 
GATGATTGTG CCTTCGCCTC CTGTACTCCA GGCTCCACCT GCATCGACCG TGTGGCCTCC 
TTCTCTTGCA TGTGCCCAGA GGGGAAGGCA GGTCTCCTGT GTCATCTGGA TGATGCATGC 
ATCAGCAATC CTTGCCACAA GGGGGCACTG TGTGACACCA ACCCCCTAAA TGGGCAATAT 
ATTTGCACCT GCCCACAAGG CTACAAAGGG GCTGACTGCA CAGAAGATGT GGATGAATGT 
TCCATGGCCA ATAGCAATCC TTGTGAGCAT GCAGGAAAAT GTGTGAACAC GGATGGCGCC 
TTCCACTGTG AGTGTCTGAA GGGTTATGCA GGACCTCGTT GTGAGATGGA CATCAATGAG 
TGCCATTCAG ACCCCTGCCA GAATGATGCT ACCTGTCTGG ATAAGATTGG AGGCTTCACA 
TGTCTGTGCA TGCCAGGTTT CAAAGGTGTG CATTGTGAAT TAGAAATAAA TGAATGTCAG 
AGCAACCCTT GTGTGAACAA TGGGCAGTGT GTGGATAAAG TCAATCGTTT CCAGTGCCTG 
TGTCCTCCTG GTTTCACTGG GCCAGTTTGC CAGATTGATA TTGATGACTG TTCCAGTACT 
CCGTGTCTGA ATGGGGCAAA GTGTATCGAT CACCCGAATG GCTATGAATG CCAGTGTGCC 
ACAGGTTTCA CTGGTGTGTT GTGTGAGGAG AACATTGACA ACTGTGACCC CGATCCTTGC 
CACCATGGTC AGTGTCAGGA TGGTATTGAT TCCTACACCT GCATCTGCAA TCCCGGGTAC 
ATGGGCGCCA TCTGCAGTGA CCAGATTGAT GAATGTTACA GCAGCCCTTG CCTGAACGAT 
GGTCGCTGCA TTGACCTGGT CAATGGCTAC CAGTGCAACT GCCAGCCGGG CACGTCAGGG 
GTTAATTGTG AAATTAATTT TGATGACTGT GCAAGTAACC CTTGTATCCA TGGAAACTGT 
ATGGATGGCA TTAATCGCTA CAGTTGTGTC TGCTCACCAG GATTCACAGG GCAGAGATGT 
AACATTGACA TTGATGAGTG TGCCTCCAAT CCCTGTCGCA AGGGTGCAAC ATGTATCAAC 
GGTGTGAATG GTTTCCGCTG TATATGCCCC GAGGGACCCC ATCACCCCAG CTGCTACTCA 
CAGGTGAACG AATGCCTGAG CAATCCCTGC ATCCATGGAA ACTGTACTGG AGGTCTCAGT 
GGATATAAGT GTCTCTGTGA TGCAGGCTGG GTTGGCATCA ACTGTGAAGT GGACAAAAAT 
GAATGCCTTT CGAATCCATG CCAGAATGGA GGAACTTGTG ACAATCTGGT GAATGGATAC 
AGGTGTACTT GCAAGAAGGG CTTTAAAGGC TATAACTGCC AGGTGAATAT TGATGAATGT 
GCCTCAAATC CATGCCTGAA CCAAGGAACC TGCTTTGATG ACATAAGTGG CTACACTTGC 
CACTGTGTGC TGCCATACAC AGGCAAGAAT TGTCAGACAG TATTGGCTCC CTGTTCCCCA 
AACCCTTGTG AGAATGCTGC TGTTTGCAAA GAGTCACCAA ATTTTGAGAG TTATACTTGC 
TTGTGTGCTC CTGGCTGGCA AGGTCAGCGG TGTACCATTG ACATTGACGA GTGTATCTCC 
AAGCCCTGCA TGAACCATGG TCTCTGCCAT AACACCCAGG GCAGCTACAT GTGTGAATGT 
CCACCAGGCT TCAGTGGTAT GGACTGTGAG GAGGACATTG ATGACTGCCT TGCCAATCCT 
TGCCAGAATG GAGGTTCCTG TGTGGATGGA GTGAATACTT TCTCCTGCCT CTGCCTTCCG 
GGTTTCACTG GGGATAAGTG CCAGACAGAC ATGAATGAGT GTCTGAGTGA ACCCTGTAAG 
AATGGAGGGA CCTGCTCTGA CTACGTCAAC AGTTACACTT GCAAGTGCCA GGCAGGATTT 
GATGGAGTCC ATTGTGAGAA CAACATCAAT GAGTGCACTG AGAGCTCCTG TTTCAATGGT 
GGCACATGTG TTGATGGGAT TAACTCCTTC TCTTGCTTGT GCCCTGTGGG TTTCACTGGA 
TCCTTCTGCC TCCATGAGAT CAATGAATGC AGCTCTCATC CATGCCTCAA TGAGGGAACG 
TGTGTTGATG GCCTGGGTAC CTACCGCTGC AGCTGCCCCC TGGGCTACAC TGGGAAAAAC 
TGTCAGACCC TGGTGAACCT CTGCAGTCGG TCTCCATGTA AAAACAAAGG TACTTGCGTT 
CAGAAAAAAG CAGAGTCCCA GTGCCTATGT CCATCTGGAT GGGCTGGTGC CTATTGTGAC 
GTGCCCAATG TCTCTTGTGA CATAGCAGCC TCCAGGAGAG GTGTGCTTGT TGAACACTTG 
TGCCAGCACT CAGGTGTCTG CATCAATGCT GGCAACACGC ATTACTGTCA GTGCCCCCTG 
GGCTATACTG GGAGCTACTG TGAGGAGCAA CTCGATGAGT GTGCGTCCAA CCCCTGCCAG 
CACGGGGCAA CATGCAGTGA CTTCATTGGT GGATACAGAT GTGAGTGTGT CCCAGGCTAT 
CAGGGTGTCA ACTGTGAGTA TGAAGTGGAT GAGTGCCAGA ATCAGCCCTG CCAGAATGGA 
GGCACCTGTA TTGACCTTGT GAACCATTTC AAGTGCTCTT GCCCACCAGG CACTCGGGGC 
CTACTCTGTG AAGAGAACAT TGATGACTGT GCCCGGGGTC CCCATTGCCT TAATGGTGGT 
CAGTGCATGG ATAGGATTGG AGGCTACAGT TGTCGCTGCT TGCCTGGCTT TGCTGGGGAG 
CGTTGTGAGG GAGACATCAA CGAGTGCCTC TCCAACCCCT GCAGCTCTGA GGGCAGCCTG 
GACTGTATAC AGCTCACCAA TGACTACCTG TGTGTTTGCC GTAGTGCCTT TACTGGCCGG 
CACTGTGAAA CCTTCGTCGA TGTGTGTCCC CAGATGCCCT GCCTGAATGG AGGGACTTGT 
GCTGTGGCCA GTAACATGCC TGATGGTTTC ATTTGCCGTT GTCCCCCGGG ATTTTCTGGG 
GCAAGGTGCC AGAGCAGCTG TGGACAAGTG AAATGTAGGA AAGGGGAGCA GTGTGTGCAC 
ACCGCCTCTG GACCCCGCTG CTTCTGCCCC AGTCCCCGGG ACTGCGAGTC AGGCTGTGCC 
AGTAGCCCCT GCCAGCACGG GGGCAGCTGC CACCCTCAGC GCCAGCCTCC TTATTACTCC 
TGCCAGTGTG CCCCACCATT CTCGGGTAGC CGCTGTGAAC TCTACACGGC ACCCCCCAGC 
ACCCCTCCTG CCACCTGTCT GAGCCAGTAT TGTGCCGACA AAGCTCGGGA TGGCGTCTGT 
GATGAGGCCT GCAACAGCCA TGCCTGCCAG TGGGATGGGG GTGACTGTTC TCTCACCATG 
GAGAACCCCT GGGCCAACTG CTCCTCCCCA CTTCCCTGCT GGGATTATAT CAACAACCAG 
TGTGATGAGC TGTGCAACAC GGTCGAGTGC CTGTTTGACA ACTTTGAATG CCAGGGGAAC 
AGCAAGACAT GCAAGTATGA CAAATACTGT GCAGACCACT TCAAAGACAA CCACTGTGAC 
CAGGGGTGCA ACAGTGAGGA GTGTGGTTGG GATGGGCTGG ACTGTGCTGC TGACCAACCT 
GAGAACCTGG CAGAAGGTAC CCTGGTTATT GTGGTATTGA TGCCACCTGA ACAACTGCTC 
CAGGATGCTC GCAGCTTCTT GCGGGCACTG GGTACCCTGC TGCACACCAA CCTGCGCATT 
AAGCGGGACT CCCAGGGGGA ACTCATGGTG TACCCCTATT ATGGTGAGAA GTCAGCTGCT 
ATGAAGAAAC AGAGGATGAC ACGCAGGTCC CTTCCTGGTG AACAAGAACA GGAGGTGGCT 
GGCTCTAAAG TCTTTCTGGA AATTGACAAC CGCCAGTGTG TTCAAGACTC AGACCACTGC 
TTCAAGAACA CGGATGCAGC AGCAGCTCTC CTGGCCTCTC ACGCCATACA GGGGACCCTG 
TCATACCCTC TTGTGTCTGT CGTCAGTGAA TCCCTGACTC CAGAACGCAC TCAGCTCCTC 
TATCTCCTTG CTGTTGCTGT TGTCATCATT CTGTTTATTA TTCTGCTGGG GGTAATCATG 
GCAAAACGAA AGCGTAAGCA TGGCTCTCTC TGGCTGCCTG AAGGTTTCAC TCTTCGCCGA 
GATGCAAGCA ATCACAAGCG TCGTGAGCCA GTGGGACAGG ATGCTGTGGG GCTAAAAAAT 
CTCTCAGTGC AAGTCTCAGA AGCTAACCTA ATTGGTTCTG GAACAAGTGA ACACTGGGTC 
GATGATGAAG GGCCCCAGCC AAAGAAAGTA AAGGCTGAAG ATGAGGCCTT ACTCTCAGAA 
GAAGATGACC CCATTGATCG ACGGCCATGG ACACAGCAGC ACCTTGAAGC CGCAGACATC 
CGTAGGACAC CATCGCTGGC TCTCACCCCT CCTCAGGCAG AGCAGGAGGT GGATGTGTTA 
GATGTGAATG TCCGTGGCCC AGATGGCTGC ACCCCATTGA TGTTGGCTTC TCTCCGAGGA 
GGCAGCTCAG ATTTGAGTGA TGAAGATGAA GATGCAGAGG ACTCTTCTGC TAACATCATC 
ACAGACTTGG TCTACCAGGG TGCCAGCCTC CAGGCCCAGA CAGACCGGAC TGGTGAGATG 
GCCCTGCACC TTGCAGCCCG CTACTCACGG GCTGATGCTG CCAAGCGTCT CCTGGATGCA 
GGTGCAGATG CCAATGCCCA GGACAACATG GGCCGCTGCC CACTCCATGC TGCAGTGGCA 
GCTGATGCCC AAGGTGTCTT CCAGATTCTG ATTCGCAACC GAGTAACTGA TCTAGATGCC 
AGGATGAATG ATGGTACTAC ACCCCTGATC CTGGCTGCCC GCCTGGCTGT GGAGGGAATG 
GTGGCAGAAC TGATCAACTG CCAAGCGGAT GTGAATGCAG TGGATGACCA TGGAAAATCT 
GCTCTTCACT GGGCAGCTGC TGTCAATAAT GTGGAGGCAA CTCTTTTGTT GTTGAAAAAT 
GGAGCCAACC GAGACATGCA GGACAACAAG GAAGAGACAC CTCTGTTTCT TGCTGCCCGG 
GAGGGGAGCT ATGAAGCAGC CAAGATCCTG TTAGACCATT TTGCCAATCG AGACATCACA 
GACCATATGG ATCGTCTTCC CCGGGATGTG GCTCGGGATC GCATGCACCA TGACATTGTG 
CGCCTTCTGG ATGAATACAA TGTGACCCCA AGCCCTCCAG GCACCGTGTT GACTTCTGCT 
CTCTCACCTG TCATCTGTGG GCCCAACAGA TCTTTCCTCA GCCTGAAGCA CACCCCAATG 
GGCAAGAAGT CTAGACGGCC CAGTGCCAAG AGTACCATGC CTACTAGCCT CCCTAACCTT 
GCCAAGGAGG CAAAGGATGC CAAGGGTAGT AGGAGGAAGA AGTCTCTGAG TGAGAAGGTC 
CAACTGTCTG AGAGTTCAGT AACTTTATCC CCTGTTGATT CCCTAGAATC TCCTCACACG 
TATGTTTCCG ACACCACATC CTCTCCAATG ATTACATCCC CTGGGATCTT ACAGGCCTCA 
CCCAACCCTA TGTTGGCCAC TGCCGCCCCT CCTGCCCCAG TCCATGCCCA GCATGCACTA 
TCTTTTTCTA ACCTTCATGA AATGCAGCCT TTGGCACATG GGGCCAGCAC TGTGCTTCCC 
TCAGTGAGCC AGTTGCTATC CCACCACCAC ATTGTGTCTC CAGGCAGTGG CAGTGCTGGA 
AGCTTGAGTA GGCTCCATCC AGTCCCAGTC CCAGCAGATT GGATGAACCG CATGGAGGTG 
AATGAGACCC AGTACAATGA GATGTTTGGT ATGGTCCTGG CTCCAGCTGA GGGCACCCAT 
CCTGGCATAG CTCCCCAGAG CAGGCCACCT GAAGGGAAGC ACATAACCAC CCCTCGGGAG 
CCCTTGCCCC CCATTGTGAC TTTCCAGCTC ATCCCTAAAG GCAGTATTGC CCAACCAGCG 
GGGGCTCCCC AGCCTCAGTC CACCTGCCCT CCAGCTGTTG CGGGCCCCCT GCCCACCATG 
TACCAGATTC CAGAAATGGC CCGTTTGCCC AGTGTGGCTT TCCCCACTGC CATGATGCCC 
CAGCAGGACG GGCAGGTAGC TCAGACCATT CTCCCAGCCT ATCATCCTTT CCCAGCCTCT 
GTGGGCAAGT ACCCCACACC CCCTTCACAG CACAGTTATG CTTCCTCAAA TGCTGCTGAG 
CGAACACCCA GTCACAGTGG TCACCTCCAG GGTGAGCATC CCTACCTGAC ACCATCCCCA 
GAGTCTCCTG ACCAGTGGTC AAGTTCATCA CCCCACTCTG CTTCTGACTG GTCAGATGTG 
ACCACCAGCC CTACCCCTGG GGGTGCTGGA GGAGGTCAGC GGGGACCTGG GACACACATG 
TCTGAGCCAC CACACAACAA CATGCAGGTT TATGCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024210696.1
CDS	224..7522
Translation	MCVTYHNGTGYCKCPEGFLGEYCQHRDPCEKNRCQNGGTCVAQAMLGKATCRCASGFTGEDCQYSTSHPCFVSRPCLNGGTCHMLSRDTYECTCQVGFTGKECQWTDACLSHPCANGSTCTTVANQFSCKCLTGFTGQKCETDVNECDIPGHCQHGGTCLNLPGSYQCQCPQGFTGQYCDSLYVPCAPSPCVNGGTCRQTGDFTFECSCLPGFEGSTCERNIDDCPNHRCQNGGVCVDGVNTYNCRCPPQWTGQFCTEDVDECLLQPNACQNGGTCANRNGGYGCVCVNGWSGDDCSENIDDCAFASCTPGSTCIDRVASFSCMCPEGKAGLLCHLDDACISNPCHKGALCDTNPLNGQYICTCPQGYKGADCTEDVDECSMANSNPCEHAGKCVNTDGAFHCECLKGYAGPRCEMDINECHSDPCQNDATCLDKIGGFTCLCMPGFKGVHCELEINECQSNPCVNNGQCVDKVNRFQCLCPPGFTGPVCQIDIDDCSSTPCLNGAKCIDHPNGYECQCATGFTGVLCEENIDNCDPDPCHHGQCQDGIDSYTCICNPGYMGAICSDQIDECYSSPCLNDGRCIDLVNGYQCNCQPGTSGVNCEINFDDCASNPCIHGNCMDGINRYSCVCSPGFTGQRCNIDIDECASNPCRKGATCINGVNGFRCICPEGPHHPSCYSQVNECLSNPCIHGNCTGGLSGYKCLCDAGWVGINCEVDKNECLSNPCQNGGTCDNLVNGYRCTCKKGFKGYNCQVNIDECASNPCLNQGTCFDDISGYTCHCVLPYTGKNCQTVLAPCSPNPCENAAVCKESPNFESYTCLCAPGWQGQRCTIDIDECISKPCMNHGLCHNTQGSYMCECPPGFSGMDCEEDIDDCLANPCQNGGSCVDGVNTFSCLCLPGFTGDKCQTDMNECLSEPCKNGGTCSDYVNSYTCKCQAGFDGVHCENNINECTESSCFNGGTCVDGINSFSCLCPVGFTGSFCLHEINECSSHPCLNEGTCVDGLGTYRCSCPLGYTGKNCQTLVNLCSRSPCKNKGTCVQKKAESQCLCPSGWAGAYCDVPNVSCDIAASRRGVLVEHLCQHSGVCINAGNTHYCQCPLGYTGSYCEEQLDECASNPCQHGATCSDFIGGYRCECVPGYQGVNCEYEVDECQNQPCQNGGTCIDLVNHFKCSCPPGTRGLLCEENIDDCARGPHCLNGGQCMDRIGGYSCRCLPGFAGERCEGDINECLSNPCSSEGSLDCIQLTNDYLCVCRSAFTGRHCETFVDVCPQMPCLNGGTCAVASNMPDGFICRCPPGFSGARCQSSCGQVKCRKGEQCVHTASGPRCFCPSPRDCESGCASSPCQHGGSCHPQRQPPYYSCQCAPPFSGSRCELYTAPPSTPPATCLSQYCADKARDGVCDEACNSHACQWDGGDCSLTMENPWANCSSPLPCWDYINNQCDELCNTVECLFDNFECQGNSKTCKYDKYCADHFKDNHCDQGCNSEECGWDGLDCAADQPENLAEGTLVIVVLMPPEQLLQDARSFLRALGTLLHTNLRIKRDSQGELMVYPYYGEKSAAMKKQRMTRRSLPGEQEQEVAGSKVFLEIDNRQCVQDSDHCFKNTDAAAALLASHAIQGTLSYPLVSVVSESLTPERTQLLYLLAVAVVIILFIILLGVIMAKRKRKHGSLWLPEGFTLRRDASNHKRREPVGQDAVGLKNLSVQVSEANLIGSGTSEHWVDDEGPQPKKVKAEDEALLSEEDDPIDRRPWTQQHLEAADIRRTPSLALTPPQAEQEVDVLDVNVRGPDGCTPLMLASLRGGSSDLSDEDEDAEDSSANIITDLVYQGASLQAQTDRTGEMALHLAARYSRADAAKRLLDAGADANAQDNMGRCPLHAAVAADAQGVFQILIRNRVTDLDARMNDGTTPLILAARLAVEGMVAELINCQADVNAVDDHGKSALHWAAAVNNVEATLLLLKNGANRDMQDNKEETPLFLAAREGSYEAAKILLDHFANRDITDHMDRLPRDVARDRMHHDIVRLLDEYNVTPSPPGTVLTSALSPVICGPNRSFLSLKHTPMGKKSRRPSAKSTMPTSLPNLAKEAKDAKGSRRKKSLSEKVQLSESSVTLSPVDSLESPHTYVSDTTSSPMITSPGILQASPNPMLATAAPPAPVHAQHALSFSNLHEMQPLAHGASTVLPSVSQLLSHHHIVSPGSGSAGSLSRLHPVPVPADWMNRMEVNETQYNEMFGMVLAPAEGTHPGIAPQSRPPEGKHITTPREPLPPIVTFQLIPKGSIAQPAGAPQPQSTCPPAVAGPLPTMYQIPEMARLPSVAFPTAMMPQQDGQVAQTILPAYHPFPASVGKYPTPPSQHSYASSNAAERTPSHSGHLQGEHPYLTPSPESPDQWSSSSPHSASDWSDVTTSPTPGGAGGGQRGPGTHMSEPPHNNMQVYA

Target ORF information:

RefSeq Version	XM_024354928.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes notch 2 (NOTCH2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024354928.1

ORF Insert Sequence:

ATGTGTGTTA CCTACCACAA TGGCACAGGA TACTGCAAAT GTCCAGAAGG CTTCTTGGGG 
GAATATTGTC AACATCGAGA CCCCTGTGAG AAGAACCGCT GCCAGAATGG TGGGACTTGT 
GTGGCCCAGG CCATGCTGGG GAAAGCCACG TGCCGATGTG CCTCAGGGTT TACAGGAGAG 
GACTGCCAGT ACTCGACATC TCATCCATGC TTTGTGTCTC GACCCTGCCT GAATGGCGGC 
ACATGCCATA TGCTCAGCCG GGATACCTAT GAGTGCACCT GTCAAGTCGG GTTTACAGGT 
AAGGAGTGCC AATGGACCGA TGCCTGCCTG TCTCATCCCT GTGCAAATGG AAGTACCTGT 
ACCACTGTGG CCAACCAGTT CTCCTGCAAA TGCCTCACAG GCTTCACAGG GCAGAAGTGT 
GAGACTGATG TCAATGAGTG TGACATTCCA GGACACTGCC AGCATGGTGG CACCTGCCTC 
AACCTGCCTG GTTCCTACCA GTGCCAGTGC CCTCAGGGCT TCACAGGCCA GTACTGTGAC 
AGCCTGTATG TGCCCTGTGC ACCCTCGCCT TGTGTCAATG GAGGCACCTG TCGGCAGACT 
GGTGACTTCA CTTTTGAGTG CAGCTGCCTT CCAGGTTTTG AAGGGAGCAC CTGTGAGAGG 
AATATTGATG ACTGCCCTAA CCACAGGTGT CAGAATGGAG GGGTTTGTGT GGATGGGGTC 
AACACTTACA ACTGCCGCTG TCCCCCACAA TGGACAGGAC AGTTCTGCAC AGAGGATGTG 
GATGAATGCC TGCTGCAGCC CAATGCCTGT CAAAATGGGG GCACCTGTGC CAACCGCAAT 
GGAGGCTATG GCTGTGTATG TGTCAACGGC TGGAGTGGAG ATGACTGCAG TGAGAACATT 
GATGATTGTG CCTTCGCCTC CTGTACTCCA GGCTCCACCT GCATCGACCG TGTGGCCTCC 
TTCTCTTGCA TGTGCCCAGA GGGGAAGGCA GGTCTCCTGT GTCATCTGGA TGATGCATGC 
ATCAGCAATC CTTGCCACAA GGGGGCACTG TGTGACACCA ACCCCCTAAA TGGGCAATAT 
ATTTGCACCT GCCCACAAGG CTACAAAGGG GCTGACTGCA CAGAAGATGT GGATGAATGT 
TCCATGGCCA ATAGCAATCC TTGTGAGCAT GCAGGAAAAT GTGTGAACAC GGATGGCGCC 
TTCCACTGTG AGTGTCTGAA GGGTTATGCA GGACCTCGTT GTGAGATGGA CATCAATGAG 
TGCCATTCAG ACCCCTGCCA GAATGATGCT ACCTGTCTGG ATAAGATTGG AGGCTTCACA 
TGTCTGTGCA TGCCAGGTTT CAAAGGTGTG CATTGTGAAT TAGAAATAAA TGAATGTCAG 
AGCAACCCTT GTGTGAACAA TGGGCAGTGT GTGGATAAAG TCAATCGTTT CCAGTGCCTG 
TGTCCTCCTG GTTTCACTGG GCCAGTTTGC CAGATTGATA TTGATGACTG TTCCAGTACT 
CCGTGTCTGA ATGGGGCAAA GTGTATCGAT CACCCGAATG GCTATGAATG CCAGTGTGCC 
ACAGGTTTCA CTGGTGTGTT GTGTGAGGAG AACATTGACA ACTGTGACCC CGATCCTTGC 
CACCATGGTC AGTGTCAGGA TGGTATTGAT TCCTACACCT GCATCTGCAA TCCCGGGTAC 
ATGGGCGCCA TCTGCAGTGA CCAGATTGAT GAATGTTACA GCAGCCCTTG CCTGAACGAT 
GGTCGCTGCA TTGACCTGGT CAATGGCTAC CAGTGCAACT GCCAGCCGGG CACGTCAGGG 
GTTAATTGTG AAATTAATTT TGATGACTGT GCAAGTAACC CTTGTATCCA TGGAAACTGT 
ATGGATGGCA TTAATCGCTA CAGTTGTGTC TGCTCACCAG GATTCACAGG GCAGAGATGT 
AACATTGACA TTGATGAGTG TGCCTCCAAT CCCTGTCGCA AGGGTGCAAC ATGTATCAAC 
GGTGTGAATG GTTTCCGCTG TATATGCCCC GAGGGACCCC ATCACCCCAG CTGCTACTCA 
CAGGTGAACG AATGCCTGAG CAATCCCTGC ATCCATGGAA ACTGTACTGG AGGTCTCAGT 
GGATATAAGT GTCTCTGTGA TGCAGGCTGG GTTGGCATCA ACTGTGAAGT GGACAAAAAT 
GAATGCCTTT CGAATCCATG CCAGAATGGA GGAACTTGTG ACAATCTGGT GAATGGATAC 
AGGTGTACTT GCAAGAAGGG CTTTAAAGGC TATAACTGCC AGGTGAATAT TGATGAATGT 
GCCTCAAATC CATGCCTGAA CCAAGGAACC TGCTTTGATG ACATAAGTGG CTACACTTGC 
CACTGTGTGC TGCCATACAC AGGCAAGAAT TGTCAGACAG TATTGGCTCC CTGTTCCCCA 
AACCCTTGTG AGAATGCTGC TGTTTGCAAA GAGTCACCAA ATTTTGAGAG TTATACTTGC 
TTGTGTGCTC CTGGCTGGCA AGGTCAGCGG TGTACCATTG ACATTGACGA GTGTATCTCC 
AAGCCCTGCA TGAACCATGG TCTCTGCCAT AACACCCAGG GCAGCTACAT GTGTGAATGT 
CCACCAGGCT TCAGTGGTAT GGACTGTGAG GAGGACATTG ATGACTGCCT TGCCAATCCT 
TGCCAGAATG GAGGTTCCTG TGTGGATGGA GTGAATACTT TCTCCTGCCT CTGCCTTCCG 
GGTTTCACTG GGGATAAGTG CCAGACAGAC ATGAATGAGT GTCTGAGTGA ACCCTGTAAG 
AATGGAGGGA CCTGCTCTGA CTACGTCAAC AGTTACACTT GCAAGTGCCA GGCAGGATTT 
GATGGAGTCC ATTGTGAGAA CAACATCAAT GAGTGCACTG AGAGCTCCTG TTTCAATGGT 
GGCACATGTG TTGATGGGAT TAACTCCTTC TCTTGCTTGT GCCCTGTGGG TTTCACTGGA 
TCCTTCTGCC TCCATGAGAT CAATGAATGC AGCTCTCATC CATGCCTCAA TGAGGGAACG 
TGTGTTGATG GCCTGGGTAC CTACCGCTGC AGCTGCCCCC TGGGCTACAC TGGGAAAAAC 
TGTCAGACCC TGGTGAACCT CTGCAGTCGG TCTCCATGTA AAAACAAAGG TACTTGCGTT 
CAGAAAAAAG CAGAGTCCCA GTGCCTATGT CCATCTGGAT GGGCTGGTGC CTATTGTGAC 
GTGCCCAATG TCTCTTGTGA CATAGCAGCC TCCAGGAGAG GTGTGCTTGT TGAACACTTG 
TGCCAGCACT CAGGTGTCTG CATCAATGCT GGCAACACGC ATTACTGTCA GTGCCCCCTG 
GGCTATACTG GGAGCTACTG TGAGGAGCAA CTCGATGAGT GTGCGTCCAA CCCCTGCCAG 
CACGGGGCAA CATGCAGTGA CTTCATTGGT GGATACAGAT GTGAGTGTGT CCCAGGCTAT 
CAGGGTGTCA ACTGTGAGTA TGAAGTGGAT GAGTGCCAGA ATCAGCCCTG CCAGAATGGA 
GGCACCTGTA TTGACCTTGT GAACCATTTC AAGTGCTCTT GCCCACCAGG CACTCGGGGC 
CTACTCTGTG AAGAGAACAT TGATGACTGT GCCCGGGGTC CCCATTGCCT TAATGGTGGT 
CAGTGCATGG ATAGGATTGG AGGCTACAGT TGTCGCTGCT TGCCTGGCTT TGCTGGGGAG 
CGTTGTGAGG GAGACATCAA CGAGTGCCTC TCCAACCCCT GCAGCTCTGA GGGCAGCCTG 
GACTGTATAC AGCTCACCAA TGACTACCTG TGTGTTTGCC GTAGTGCCTT TACTGGCCGG 
CACTGTGAAA CCTTCGTCGA TGTGTGTCCC CAGATGCCCT GCCTGAATGG AGGGACTTGT 
GCTGTGGCCA GTAACATGCC TGATGGTTTC ATTTGCCGTT GTCCCCCGGG ATTTTCTGGG 
GCAAGGTGCC AGAGCAGCTG TGGACAAGTG AAATGTAGGA AAGGGGAGCA GTGTGTGCAC 
ACCGCCTCTG GACCCCGCTG CTTCTGCCCC AGTCCCCGGG ACTGCGAGTC AGGCTGTGCC 
AGTAGCCCCT GCCAGCACGG GGGCAGCTGC CACCCTCAGC GCCAGCCTCC TTATTACTCC 
TGCCAGTGTG CCCCACCATT CTCGGGTAGC CGCTGTGAAC TCTACACGGC ACCCCCCAGC 
ACCCCTCCTG CCACCTGTCT GAGCCAGTAT TGTGCCGACA AAGCTCGGGA TGGCGTCTGT 
GATGAGGCCT GCAACAGCCA TGCCTGCCAG TGGGATGGGG GTGACTGTTC TCTCACCATG 
GAGAACCCCT GGGCCAACTG CTCCTCCCCA CTTCCCTGCT GGGATTATAT CAACAACCAG 
TGTGATGAGC TGTGCAACAC GGTCGAGTGC CTGTTTGACA ACTTTGAATG CCAGGGGAAC 
AGCAAGACAT GCAAGTATGA CAAATACTGT GCAGACCACT TCAAAGACAA CCACTGTGAC 
CAGGGGTGCA ACAGTGAGGA GTGTGGTTGG GATGGGCTGG ACTGTGCTGC TGACCAACCT 
GAGAACCTGG CAGAAGGTAC CCTGGTTATT GTGGTATTGA TGCCACCTGA ACAACTGCTC 
CAGGATGCTC GCAGCTTCTT GCGGGCACTG GGTACCCTGC TGCACACCAA CCTGCGCATT 
AAGCGGGACT CCCAGGGGGA ACTCATGGTG TACCCCTATT ATGGTGAGAA GTCAGCTGCT 
ATGAAGAAAC AGAGGATGAC ACGCAGGTCC CTTCCTGGTG AACAAGAACA GGAGGTGGCT 
GGCTCTAAAG TCTTTCTGGA AATTGACAAC CGCCAGTGTG TTCAAGACTC AGACCACTGC 
TTCAAGAACA CGGATGCAGC AGCAGCTCTC CTGGCCTCTC ACGCCATACA GGGGACCCTG 
TCATACCCTC TTGTGTCTGT CGTCAGTGAA TCCCTGACTC CAGAACGCAC TCAGCTCCTC 
TATCTCCTTG CTGTTGCTGT TGTCATCATT CTGTTTATTA TTCTGCTGGG GGTAATCATG 
GCAAAACGAA AGCGTAAGCA TGGCTCTCTC TGGCTGCCTG AAGGTTTCAC TCTTCGCCGA 
GATGCAAGCA ATCACAAGCG TCGTGAGCCA GTGGGACAGG ATGCTGTGGG GCTAAAAAAT 
CTCTCAGTGC AAGTCTCAGA AGCTAACCTA ATTGGTTCTG GAACAAGTGA ACACTGGGTC 
GATGATGAAG GGCCCCAGCC AAAGAAAGTA AAGGCTGAAG ATGAGGCCTT ACTCTCAGAA 
GAAGATGACC CCATTGATCG ACGGCCATGG ACACAGCAGC ACCTTGAAGC CGCAGACATC 
CGTAGGACAC CATCGCTGGC TCTCACCCCT CCTCAGGCAG AGCAGGAGGT GGATGTGTTA 
GATGTGAATG TCCGTGGCCC AGATGGCTGC ACCCCATTGA TGTTGGCTTC TCTCCGAGGA 
GGCAGCTCAG ATTTGAGTGA TGAAGATGAA GATGCAGAGG ACTCTTCTGC TAACATCATC 
ACAGACTTGG TCTACCAGGG TGCCAGCCTC CAGGCCCAGA CAGACCGGAC TGGTGAGATG 
GCCCTGCACC TTGCAGCCCG CTACTCACGG GCTGATGCTG CCAAGCGTCT CCTGGATGCA 
GGTGCAGATG CCAATGCCCA GGACAACATG GGCCGCTGCC CACTCCATGC TGCAGTGGCA 
GCTGATGCCC AAGGTGTCTT CCAGATTCTG ATTCGCAACC GAGTAACTGA TCTAGATGCC 
AGGATGAATG ATGGTACTAC ACCCCTGATC CTGGCTGCCC GCCTGGCTGT GGAGGGAATG 
GTGGCAGAAC TGATCAACTG CCAAGCGGAT GTGAATGCAG TGGATGACCA TGGAAAATCT 
GCTCTTCACT GGGCAGCTGC TGTCAATAAT GTGGAGGCAA CTCTTTTGTT GTTGAAAAAT 
GGAGCCAACC GAGACATGCA GGACAACAAG GAAGAGACAC CTCTGTTTCT TGCTGCCCGG 
GAGGGGAGCT ATGAAGCAGC CAAGATCCTG TTAGACCATT TTGCCAATCG AGACATCACA 
GACCATATGG ATCGTCTTCC CCGGGATGTG GCTCGGGATC GCATGCACCA TGACATTGTG 
CGCCTTCTGG ATGAATACAA TGTGACCCCA AGCCCTCCAG GCACCGTGTT GACTTCTGCT 
CTCTCACCTG TCATCTGTGG GCCCAACAGA TCTTTCCTCA GCCTGAAGCA CACCCCAATG 
GGCAAGAAGT CTAGACGGCC CAGTGCCAAG AGTACCATGC CTACTAGCCT CCCTAACCTT 
GCCAAGGAGG CAAAGGATGC CAAGGGTAGT AGGAGGAAGA AGTCTCTGAG TGAGAAGGTC 
CAACTGTCTG AGAGTTCAGT AACTTTATCC CCTGTTGATT CCCTAGAATC TCCTCACACG 
TATGTTTCCG ACACCACATC CTCTCCAATG ATTACATCCC CTGGGATCTT ACAGGCCTCA 
CCCAACCCTA TGTTGGCCAC TGCCGCCCCT CCTGCCCCAG TCCATGCCCA GCATGCACTA 
TCTTTTTCTA ACCTTCATGA AATGCAGCCT TTGGCACATG GGGCCAGCAC TGTGCTTCCC 
TCAGTGAGCC AGTTGCTATC CCACCACCAC ATTGTGTCTC CAGGCAGTGG CAGTGCTGGA 
AGCTTGAGTA GGCTCCATCC AGTCCCAGTC CCAGCAGATT GGATGAACCG CATGGAGGTG 
AATGAGACCC AGTACAATGA GATGTTTGGT ATGGTCCTGG CTCCAGCTGA GGGCACCCAT 
CCTGGCATAG CTCCCCAGAG CAGGCCACCT GAAGGGAAGC ACATAACCAC CCCTCGGGAG 
CCCTTGCCCC CCATTGTGAC TTTCCAGCTC ATCCCTAAAG GCAGTATTGC CCAACCAGCG 
GGGGCTCCCC AGCCTCAGTC CACCTGCCCT CCAGCTGTTG CGGGCCCCCT GCCCACCATG 
TACCAGATTC CAGAAATGGC CCGTTTGCCC AGTGTGGCTT TCCCCACTGC CATGATGCCC 
CAGCAGGACG GGCAGGTAGC TCAGACCATT CTCCCAGCCT ATCATCCTTT CCCAGCCTCT 
GTGGGCAAGT ACCCCACACC CCCTTCACAG CACAGTTATG CTTCCTCAAA TGCTGCTGAG 
CGAACACCCA GTCACAGTGG TCACCTCCAG GGTGAGCATC CCTACCTGAC ACCATCCCCA 
GAGTCTCCTG ACCAGTGGTC AAGTTCATCA CCCCACTCTG CTTCTGACTG GTCAGATGTG 
ACCACCAGCC CTACCCCTGG GGGTGCTGGA GGAGGTCAGC GGGGACCTGG GACACACATG 
TCTGAGCCAC CACACAACAA CATGCAGGTT TATGCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NOTCH2 ( XM_024354927.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_024210695.1 Pan troglodytes notch 2 (NOTCH2), transcript variant X2, mRNA.

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CloneID	OPc92101
Clone ID Related Accession (Same CDS sequence)	XM_024354928.1 , XM_024354927.1
Accession Version	XM_024354927.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7299bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	neurogenic locus notch homolog protein 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036879.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTGTTA CCTACCACAA TGGCACAGGA TACTGCAAAT GTCCAGAAGG CTTCTTGGGG 
GAATATTGTC AACATCGAGA CCCCTGTGAG AAGAACCGCT GCCAGAATGG TGGGACTTGT 
GTGGCCCAGG CCATGCTGGG GAAAGCCACG TGCCGATGTG CCTCAGGGTT TACAGGAGAG 
GACTGCCAGT ACTCGACATC TCATCCATGC TTTGTGTCTC GACCCTGCCT GAATGGCGGC 
ACATGCCATA TGCTCAGCCG GGATACCTAT GAGTGCACCT GTCAAGTCGG GTTTACAGGT 
AAGGAGTGCC AATGGACCGA TGCCTGCCTG TCTCATCCCT GTGCAAATGG AAGTACCTGT 
ACCACTGTGG CCAACCAGTT CTCCTGCAAA TGCCTCACAG GCTTCACAGG GCAGAAGTGT 
GAGACTGATG TCAATGAGTG TGACATTCCA GGACACTGCC AGCATGGTGG CACCTGCCTC 
AACCTGCCTG GTTCCTACCA GTGCCAGTGC CCTCAGGGCT TCACAGGCCA GTACTGTGAC 
AGCCTGTATG TGCCCTGTGC ACCCTCGCCT TGTGTCAATG GAGGCACCTG TCGGCAGACT 
GGTGACTTCA CTTTTGAGTG CAGCTGCCTT CCAGGTTTTG AAGGGAGCAC CTGTGAGAGG 
AATATTGATG ACTGCCCTAA CCACAGGTGT CAGAATGGAG GGGTTTGTGT GGATGGGGTC 
AACACTTACA ACTGCCGCTG TCCCCCACAA TGGACAGGAC AGTTCTGCAC AGAGGATGTG 
GATGAATGCC TGCTGCAGCC CAATGCCTGT CAAAATGGGG GCACCTGTGC CAACCGCAAT 
GGAGGCTATG GCTGTGTATG TGTCAACGGC TGGAGTGGAG ATGACTGCAG TGAGAACATT 
GATGATTGTG CCTTCGCCTC CTGTACTCCA GGCTCCACCT GCATCGACCG TGTGGCCTCC 
TTCTCTTGCA TGTGCCCAGA GGGGAAGGCA GGTCTCCTGT GTCATCTGGA TGATGCATGC 
ATCAGCAATC CTTGCCACAA GGGGGCACTG TGTGACACCA ACCCCCTAAA TGGGCAATAT 
ATTTGCACCT GCCCACAAGG CTACAAAGGG GCTGACTGCA CAGAAGATGT GGATGAATGT 
TCCATGGCCA ATAGCAATCC TTGTGAGCAT GCAGGAAAAT GTGTGAACAC GGATGGCGCC 
TTCCACTGTG AGTGTCTGAA GGGTTATGCA GGACCTCGTT GTGAGATGGA CATCAATGAG 
TGCCATTCAG ACCCCTGCCA GAATGATGCT ACCTGTCTGG ATAAGATTGG AGGCTTCACA 
TGTCTGTGCA TGCCAGGTTT CAAAGGTGTG CATTGTGAAT TAGAAATAAA TGAATGTCAG 
AGCAACCCTT GTGTGAACAA TGGGCAGTGT GTGGATAAAG TCAATCGTTT CCAGTGCCTG 
TGTCCTCCTG GTTTCACTGG GCCAGTTTGC CAGATTGATA TTGATGACTG TTCCAGTACT 
CCGTGTCTGA ATGGGGCAAA GTGTATCGAT CACCCGAATG GCTATGAATG CCAGTGTGCC 
ACAGGTTTCA CTGGTGTGTT GTGTGAGGAG AACATTGACA ACTGTGACCC CGATCCTTGC 
CACCATGGTC AGTGTCAGGA TGGTATTGAT TCCTACACCT GCATCTGCAA TCCCGGGTAC 
ATGGGCGCCA TCTGCAGTGA CCAGATTGAT GAATGTTACA GCAGCCCTTG CCTGAACGAT 
GGTCGCTGCA TTGACCTGGT CAATGGCTAC CAGTGCAACT GCCAGCCGGG CACGTCAGGG 
GTTAATTGTG AAATTAATTT TGATGACTGT GCAAGTAACC CTTGTATCCA TGGAAACTGT 
ATGGATGGCA TTAATCGCTA CAGTTGTGTC TGCTCACCAG GATTCACAGG GCAGAGATGT 
AACATTGACA TTGATGAGTG TGCCTCCAAT CCCTGTCGCA AGGGTGCAAC ATGTATCAAC 
GGTGTGAATG GTTTCCGCTG TATATGCCCC GAGGGACCCC ATCACCCCAG CTGCTACTCA 
CAGGTGAACG AATGCCTGAG CAATCCCTGC ATCCATGGAA ACTGTACTGG AGGTCTCAGT 
GGATATAAGT GTCTCTGTGA TGCAGGCTGG GTTGGCATCA ACTGTGAAGT GGACAAAAAT 
GAATGCCTTT CGAATCCATG CCAGAATGGA GGAACTTGTG ACAATCTGGT GAATGGATAC 
AGGTGTACTT GCAAGAAGGG CTTTAAAGGC TATAACTGCC AGGTGAATAT TGATGAATGT 
GCCTCAAATC CATGCCTGAA CCAAGGAACC TGCTTTGATG ACATAAGTGG CTACACTTGC 
CACTGTGTGC TGCCATACAC AGGCAAGAAT TGTCAGACAG TATTGGCTCC CTGTTCCCCA 
AACCCTTGTG AGAATGCTGC TGTTTGCAAA GAGTCACCAA ATTTTGAGAG TTATACTTGC 
TTGTGTGCTC CTGGCTGGCA AGGTCAGCGG TGTACCATTG ACATTGACGA GTGTATCTCC 
AAGCCCTGCA TGAACCATGG TCTCTGCCAT AACACCCAGG GCAGCTACAT GTGTGAATGT 
CCACCAGGCT TCAGTGGTAT GGACTGTGAG GAGGACATTG ATGACTGCCT TGCCAATCCT 
TGCCAGAATG GAGGTTCCTG TGTGGATGGA GTGAATACTT TCTCCTGCCT CTGCCTTCCG 
GGTTTCACTG GGGATAAGTG CCAGACAGAC ATGAATGAGT GTCTGAGTGA ACCCTGTAAG 
AATGGAGGGA CCTGCTCTGA CTACGTCAAC AGTTACACTT GCAAGTGCCA GGCAGGATTT 
GATGGAGTCC ATTGTGAGAA CAACATCAAT GAGTGCACTG AGAGCTCCTG TTTCAATGGT 
GGCACATGTG TTGATGGGAT TAACTCCTTC TCTTGCTTGT GCCCTGTGGG TTTCACTGGA 
TCCTTCTGCC TCCATGAGAT CAATGAATGC AGCTCTCATC CATGCCTCAA TGAGGGAACG 
TGTGTTGATG GCCTGGGTAC CTACCGCTGC AGCTGCCCCC TGGGCTACAC TGGGAAAAAC 
TGTCAGACCC TGGTGAACCT CTGCAGTCGG TCTCCATGTA AAAACAAAGG TACTTGCGTT 
CAGAAAAAAG CAGAGTCCCA GTGCCTATGT CCATCTGGAT GGGCTGGTGC CTATTGTGAC 
GTGCCCAATG TCTCTTGTGA CATAGCAGCC TCCAGGAGAG GTGTGCTTGT TGAACACTTG 
TGCCAGCACT CAGGTGTCTG CATCAATGCT GGCAACACGC ATTACTGTCA GTGCCCCCTG 
GGCTATACTG GGAGCTACTG TGAGGAGCAA CTCGATGAGT GTGCGTCCAA CCCCTGCCAG 
CACGGGGCAA CATGCAGTGA CTTCATTGGT GGATACAGAT GTGAGTGTGT CCCAGGCTAT 
CAGGGTGTCA ACTGTGAGTA TGAAGTGGAT GAGTGCCAGA ATCAGCCCTG CCAGAATGGA 
GGCACCTGTA TTGACCTTGT GAACCATTTC AAGTGCTCTT GCCCACCAGG CACTCGGGGC 
CTACTCTGTG AAGAGAACAT TGATGACTGT GCCCGGGGTC CCCATTGCCT TAATGGTGGT 
CAGTGCATGG ATAGGATTGG AGGCTACAGT TGTCGCTGCT TGCCTGGCTT TGCTGGGGAG 
CGTTGTGAGG GAGACATCAA CGAGTGCCTC TCCAACCCCT GCAGCTCTGA GGGCAGCCTG 
GACTGTATAC AGCTCACCAA TGACTACCTG TGTGTTTGCC GTAGTGCCTT TACTGGCCGG 
CACTGTGAAA CCTTCGTCGA TGTGTGTCCC CAGATGCCCT GCCTGAATGG AGGGACTTGT 
GCTGTGGCCA GTAACATGCC TGATGGTTTC ATTTGCCGTT GTCCCCCGGG ATTTTCTGGG 
GCAAGGTGCC AGAGCAGCTG TGGACAAGTG AAATGTAGGA AAGGGGAGCA GTGTGTGCAC 
ACCGCCTCTG GACCCCGCTG CTTCTGCCCC AGTCCCCGGG ACTGCGAGTC AGGCTGTGCC 
AGTAGCCCCT GCCAGCACGG GGGCAGCTGC CACCCTCAGC GCCAGCCTCC TTATTACTCC 
TGCCAGTGTG CCCCACCATT CTCGGGTAGC CGCTGTGAAC TCTACACGGC ACCCCCCAGC 
ACCCCTCCTG CCACCTGTCT GAGCCAGTAT TGTGCCGACA AAGCTCGGGA TGGCGTCTGT 
GATGAGGCCT GCAACAGCCA TGCCTGCCAG TGGGATGGGG GTGACTGTTC TCTCACCATG 
GAGAACCCCT GGGCCAACTG CTCCTCCCCA CTTCCCTGCT GGGATTATAT CAACAACCAG 
TGTGATGAGC TGTGCAACAC GGTCGAGTGC CTGTTTGACA ACTTTGAATG CCAGGGGAAC 
AGCAAGACAT GCAAGTATGA CAAATACTGT GCAGACCACT TCAAAGACAA CCACTGTGAC 
CAGGGGTGCA ACAGTGAGGA GTGTGGTTGG GATGGGCTGG ACTGTGCTGC TGACCAACCT 
GAGAACCTGG CAGAAGGTAC CCTGGTTATT GTGGTATTGA TGCCACCTGA ACAACTGCTC 
CAGGATGCTC GCAGCTTCTT GCGGGCACTG GGTACCCTGC TGCACACCAA CCTGCGCATT 
AAGCGGGACT CCCAGGGGGA ACTCATGGTG TACCCCTATT ATGGTGAGAA GTCAGCTGCT 
ATGAAGAAAC AGAGGATGAC ACGCAGGTCC CTTCCTGGTG AACAAGAACA GGAGGTGGCT 
GGCTCTAAAG TCTTTCTGGA AATTGACAAC CGCCAGTGTG TTCAAGACTC AGACCACTGC 
TTCAAGAACA CGGATGCAGC AGCAGCTCTC CTGGCCTCTC ACGCCATACA GGGGACCCTG 
TCATACCCTC TTGTGTCTGT CGTCAGTGAA TCCCTGACTC CAGAACGCAC TCAGCTCCTC 
TATCTCCTTG CTGTTGCTGT TGTCATCATT CTGTTTATTA TTCTGCTGGG GGTAATCATG 
GCAAAACGAA AGCGTAAGCA TGGCTCTCTC TGGCTGCCTG AAGGTTTCAC TCTTCGCCGA 
GATGCAAGCA ATCACAAGCG TCGTGAGCCA GTGGGACAGG ATGCTGTGGG GCTAAAAAAT 
CTCTCAGTGC AAGTCTCAGA AGCTAACCTA ATTGGTTCTG GAACAAGTGA ACACTGGGTC 
GATGATGAAG GGCCCCAGCC AAAGAAAGTA AAGGCTGAAG ATGAGGCCTT ACTCTCAGAA 
GAAGATGACC CCATTGATCG ACGGCCATGG ACACAGCAGC ACCTTGAAGC CGCAGACATC 
CGTAGGACAC CATCGCTGGC TCTCACCCCT CCTCAGGCAG AGCAGGAGGT GGATGTGTTA 
GATGTGAATG TCCGTGGCCC AGATGGCTGC ACCCCATTGA TGTTGGCTTC TCTCCGAGGA 
GGCAGCTCAG ATTTGAGTGA TGAAGATGAA GATGCAGAGG ACTCTTCTGC TAACATCATC 
ACAGACTTGG TCTACCAGGG TGCCAGCCTC CAGGCCCAGA CAGACCGGAC TGGTGAGATG 
GCCCTGCACC TTGCAGCCCG CTACTCACGG GCTGATGCTG CCAAGCGTCT CCTGGATGCA 
GGTGCAGATG CCAATGCCCA GGACAACATG GGCCGCTGCC CACTCCATGC TGCAGTGGCA 
GCTGATGCCC AAGGTGTCTT CCAGATTCTG ATTCGCAACC GAGTAACTGA TCTAGATGCC 
AGGATGAATG ATGGTACTAC ACCCCTGATC CTGGCTGCCC GCCTGGCTGT GGAGGGAATG 
GTGGCAGAAC TGATCAACTG CCAAGCGGAT GTGAATGCAG TGGATGACCA TGGAAAATCT 
GCTCTTCACT GGGCAGCTGC TGTCAATAAT GTGGAGGCAA CTCTTTTGTT GTTGAAAAAT 
GGAGCCAACC GAGACATGCA GGACAACAAG GAAGAGACAC CTCTGTTTCT TGCTGCCCGG 
GAGGGGAGCT ATGAAGCAGC CAAGATCCTG TTAGACCATT TTGCCAATCG AGACATCACA 
GACCATATGG ATCGTCTTCC CCGGGATGTG GCTCGGGATC GCATGCACCA TGACATTGTG 
CGCCTTCTGG ATGAATACAA TGTGACCCCA AGCCCTCCAG GCACCGTGTT GACTTCTGCT 
CTCTCACCTG TCATCTGTGG GCCCAACAGA TCTTTCCTCA GCCTGAAGCA CACCCCAATG 
GGCAAGAAGT CTAGACGGCC CAGTGCCAAG AGTACCATGC CTACTAGCCT CCCTAACCTT 
GCCAAGGAGG CAAAGGATGC CAAGGGTAGT AGGAGGAAGA AGTCTCTGAG TGAGAAGGTC 
CAACTGTCTG AGAGTTCAGT AACTTTATCC CCTGTTGATT CCCTAGAATC TCCTCACACG 
TATGTTTCCG ACACCACATC CTCTCCAATG ATTACATCCC CTGGGATCTT ACAGGCCTCA 
CCCAACCCTA TGTTGGCCAC TGCCGCCCCT CCTGCCCCAG TCCATGCCCA GCATGCACTA 
TCTTTTTCTA ACCTTCATGA AATGCAGCCT TTGGCACATG GGGCCAGCAC TGTGCTTCCC 
TCAGTGAGCC AGTTGCTATC CCACCACCAC ATTGTGTCTC CAGGCAGTGG CAGTGCTGGA 
AGCTTGAGTA GGCTCCATCC AGTCCCAGTC CCAGCAGATT GGATGAACCG CATGGAGGTG 
AATGAGACCC AGTACAATGA GATGTTTGGT ATGGTCCTGG CTCCAGCTGA GGGCACCCAT 
CCTGGCATAG CTCCCCAGAG CAGGCCACCT GAAGGGAAGC ACATAACCAC CCCTCGGGAG 
CCCTTGCCCC CCATTGTGAC TTTCCAGCTC ATCCCTAAAG GCAGTATTGC CCAACCAGCG 
GGGGCTCCCC AGCCTCAGTC CACCTGCCCT CCAGCTGTTG CGGGCCCCCT GCCCACCATG 
TACCAGATTC CAGAAATGGC CCGTTTGCCC AGTGTGGCTT TCCCCACTGC CATGATGCCC 
CAGCAGGACG GGCAGGTAGC TCAGACCATT CTCCCAGCCT ATCATCCTTT CCCAGCCTCT 
GTGGGCAAGT ACCCCACACC CCCTTCACAG CACAGTTATG CTTCCTCAAA TGCTGCTGAG 
CGAACACCCA GTCACAGTGG TCACCTCCAG GGTGAGCATC CCTACCTGAC ACCATCCCCA 
GAGTCTCCTG ACCAGTGGTC AAGTTCATCA CCCCACTCTG CTTCTGACTG GTCAGATGTG 
ACCACCAGCC CTACCCCTGG GGGTGCTGGA GGAGGTCAGC GGGGACCTGG GACACACATG 
TCTGAGCCAC CACACAACAA CATGCAGGTT TATGCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024210695.1
CDS	289..7587
Translation	MCVTYHNGTGYCKCPEGFLGEYCQHRDPCEKNRCQNGGTCVAQAMLGKATCRCASGFTGEDCQYSTSHPCFVSRPCLNGGTCHMLSRDTYECTCQVGFTGKECQWTDACLSHPCANGSTCTTVANQFSCKCLTGFTGQKCETDVNECDIPGHCQHGGTCLNLPGSYQCQCPQGFTGQYCDSLYVPCAPSPCVNGGTCRQTGDFTFECSCLPGFEGSTCERNIDDCPNHRCQNGGVCVDGVNTYNCRCPPQWTGQFCTEDVDECLLQPNACQNGGTCANRNGGYGCVCVNGWSGDDCSENIDDCAFASCTPGSTCIDRVASFSCMCPEGKAGLLCHLDDACISNPCHKGALCDTNPLNGQYICTCPQGYKGADCTEDVDECSMANSNPCEHAGKCVNTDGAFHCECLKGYAGPRCEMDINECHSDPCQNDATCLDKIGGFTCLCMPGFKGVHCELEINECQSNPCVNNGQCVDKVNRFQCLCPPGFTGPVCQIDIDDCSSTPCLNGAKCIDHPNGYECQCATGFTGVLCEENIDNCDPDPCHHGQCQDGIDSYTCICNPGYMGAICSDQIDECYSSPCLNDGRCIDLVNGYQCNCQPGTSGVNCEINFDDCASNPCIHGNCMDGINRYSCVCSPGFTGQRCNIDIDECASNPCRKGATCINGVNGFRCICPEGPHHPSCYSQVNECLSNPCIHGNCTGGLSGYKCLCDAGWVGINCEVDKNECLSNPCQNGGTCDNLVNGYRCTCKKGFKGYNCQVNIDECASNPCLNQGTCFDDISGYTCHCVLPYTGKNCQTVLAPCSPNPCENAAVCKESPNFESYTCLCAPGWQGQRCTIDIDECISKPCMNHGLCHNTQGSYMCECPPGFSGMDCEEDIDDCLANPCQNGGSCVDGVNTFSCLCLPGFTGDKCQTDMNECLSEPCKNGGTCSDYVNSYTCKCQAGFDGVHCENNINECTESSCFNGGTCVDGINSFSCLCPVGFTGSFCLHEINECSSHPCLNEGTCVDGLGTYRCSCPLGYTGKNCQTLVNLCSRSPCKNKGTCVQKKAESQCLCPSGWAGAYCDVPNVSCDIAASRRGVLVEHLCQHSGVCINAGNTHYCQCPLGYTGSYCEEQLDECASNPCQHGATCSDFIGGYRCECVPGYQGVNCEYEVDECQNQPCQNGGTCIDLVNHFKCSCPPGTRGLLCEENIDDCARGPHCLNGGQCMDRIGGYSCRCLPGFAGERCEGDINECLSNPCSSEGSLDCIQLTNDYLCVCRSAFTGRHCETFVDVCPQMPCLNGGTCAVASNMPDGFICRCPPGFSGARCQSSCGQVKCRKGEQCVHTASGPRCFCPSPRDCESGCASSPCQHGGSCHPQRQPPYYSCQCAPPFSGSRCELYTAPPSTPPATCLSQYCADKARDGVCDEACNSHACQWDGGDCSLTMENPWANCSSPLPCWDYINNQCDELCNTVECLFDNFECQGNSKTCKYDKYCADHFKDNHCDQGCNSEECGWDGLDCAADQPENLAEGTLVIVVLMPPEQLLQDARSFLRALGTLLHTNLRIKRDSQGELMVYPYYGEKSAAMKKQRMTRRSLPGEQEQEVAGSKVFLEIDNRQCVQDSDHCFKNTDAAAALLASHAIQGTLSYPLVSVVSESLTPERTQLLYLLAVAVVIILFIILLGVIMAKRKRKHGSLWLPEGFTLRRDASNHKRREPVGQDAVGLKNLSVQVSEANLIGSGTSEHWVDDEGPQPKKVKAEDEALLSEEDDPIDRRPWTQQHLEAADIRRTPSLALTPPQAEQEVDVLDVNVRGPDGCTPLMLASLRGGSSDLSDEDEDAEDSSANIITDLVYQGASLQAQTDRTGEMALHLAARYSRADAAKRLLDAGADANAQDNMGRCPLHAAVAADAQGVFQILIRNRVTDLDARMNDGTTPLILAARLAVEGMVAELINCQADVNAVDDHGKSALHWAAAVNNVEATLLLLKNGANRDMQDNKEETPLFLAAREGSYEAAKILLDHFANRDITDHMDRLPRDVARDRMHHDIVRLLDEYNVTPSPPGTVLTSALSPVICGPNRSFLSLKHTPMGKKSRRPSAKSTMPTSLPNLAKEAKDAKGSRRKKSLSEKVQLSESSVTLSPVDSLESPHTYVSDTTSSPMITSPGILQASPNPMLATAAPPAPVHAQHALSFSNLHEMQPLAHGASTVLPSVSQLLSHHHIVSPGSGSAGSLSRLHPVPVPADWMNRMEVNETQYNEMFGMVLAPAEGTHPGIAPQSRPPEGKHITTPREPLPPIVTFQLIPKGSIAQPAGAPQPQSTCPPAVAGPLPTMYQIPEMARLPSVAFPTAMMPQQDGQVAQTILPAYHPFPASVGKYPTPPSQHSYASSNAAERTPSHSGHLQGEHPYLTPSPESPDQWSSSSPHSASDWSDVTTSPTPGGAGGGQRGPGTHMSEPPHNNMQVYA

Target ORF information:

RefSeq Version	XM_024354927.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes notch 2 (NOTCH2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024354927.1

ORF Insert Sequence:

ATGTGTGTTA CCTACCACAA TGGCACAGGA TACTGCAAAT GTCCAGAAGG CTTCTTGGGG 
GAATATTGTC AACATCGAGA CCCCTGTGAG AAGAACCGCT GCCAGAATGG TGGGACTTGT 
GTGGCCCAGG CCATGCTGGG GAAAGCCACG TGCCGATGTG CCTCAGGGTT TACAGGAGAG 
GACTGCCAGT ACTCGACATC TCATCCATGC TTTGTGTCTC GACCCTGCCT GAATGGCGGC 
ACATGCCATA TGCTCAGCCG GGATACCTAT GAGTGCACCT GTCAAGTCGG GTTTACAGGT 
AAGGAGTGCC AATGGACCGA TGCCTGCCTG TCTCATCCCT GTGCAAATGG AAGTACCTGT 
ACCACTGTGG CCAACCAGTT CTCCTGCAAA TGCCTCACAG GCTTCACAGG GCAGAAGTGT 
GAGACTGATG TCAATGAGTG TGACATTCCA GGACACTGCC AGCATGGTGG CACCTGCCTC 
AACCTGCCTG GTTCCTACCA GTGCCAGTGC CCTCAGGGCT TCACAGGCCA GTACTGTGAC 
AGCCTGTATG TGCCCTGTGC ACCCTCGCCT TGTGTCAATG GAGGCACCTG TCGGCAGACT 
GGTGACTTCA CTTTTGAGTG CAGCTGCCTT CCAGGTTTTG AAGGGAGCAC CTGTGAGAGG 
AATATTGATG ACTGCCCTAA CCACAGGTGT CAGAATGGAG GGGTTTGTGT GGATGGGGTC 
AACACTTACA ACTGCCGCTG TCCCCCACAA TGGACAGGAC AGTTCTGCAC AGAGGATGTG 
GATGAATGCC TGCTGCAGCC CAATGCCTGT CAAAATGGGG GCACCTGTGC CAACCGCAAT 
GGAGGCTATG GCTGTGTATG TGTCAACGGC TGGAGTGGAG ATGACTGCAG TGAGAACATT 
GATGATTGTG CCTTCGCCTC CTGTACTCCA GGCTCCACCT GCATCGACCG TGTGGCCTCC 
TTCTCTTGCA TGTGCCCAGA GGGGAAGGCA GGTCTCCTGT GTCATCTGGA TGATGCATGC 
ATCAGCAATC CTTGCCACAA GGGGGCACTG TGTGACACCA ACCCCCTAAA TGGGCAATAT 
ATTTGCACCT GCCCACAAGG CTACAAAGGG GCTGACTGCA CAGAAGATGT GGATGAATGT 
TCCATGGCCA ATAGCAATCC TTGTGAGCAT GCAGGAAAAT GTGTGAACAC GGATGGCGCC 
TTCCACTGTG AGTGTCTGAA GGGTTATGCA GGACCTCGTT GTGAGATGGA CATCAATGAG 
TGCCATTCAG ACCCCTGCCA GAATGATGCT ACCTGTCTGG ATAAGATTGG AGGCTTCACA 
TGTCTGTGCA TGCCAGGTTT CAAAGGTGTG CATTGTGAAT TAGAAATAAA TGAATGTCAG 
AGCAACCCTT GTGTGAACAA TGGGCAGTGT GTGGATAAAG TCAATCGTTT CCAGTGCCTG 
TGTCCTCCTG GTTTCACTGG GCCAGTTTGC CAGATTGATA TTGATGACTG TTCCAGTACT 
CCGTGTCTGA ATGGGGCAAA GTGTATCGAT CACCCGAATG GCTATGAATG CCAGTGTGCC 
ACAGGTTTCA CTGGTGTGTT GTGTGAGGAG AACATTGACA ACTGTGACCC CGATCCTTGC 
CACCATGGTC AGTGTCAGGA TGGTATTGAT TCCTACACCT GCATCTGCAA TCCCGGGTAC 
ATGGGCGCCA TCTGCAGTGA CCAGATTGAT GAATGTTACA GCAGCCCTTG CCTGAACGAT 
GGTCGCTGCA TTGACCTGGT CAATGGCTAC CAGTGCAACT GCCAGCCGGG CACGTCAGGG 
GTTAATTGTG AAATTAATTT TGATGACTGT GCAAGTAACC CTTGTATCCA TGGAAACTGT 
ATGGATGGCA TTAATCGCTA CAGTTGTGTC TGCTCACCAG GATTCACAGG GCAGAGATGT 
AACATTGACA TTGATGAGTG TGCCTCCAAT CCCTGTCGCA AGGGTGCAAC ATGTATCAAC 
GGTGTGAATG GTTTCCGCTG TATATGCCCC GAGGGACCCC ATCACCCCAG CTGCTACTCA 
CAGGTGAACG AATGCCTGAG CAATCCCTGC ATCCATGGAA ACTGTACTGG AGGTCTCAGT 
GGATATAAGT GTCTCTGTGA TGCAGGCTGG GTTGGCATCA ACTGTGAAGT GGACAAAAAT 
GAATGCCTTT CGAATCCATG CCAGAATGGA GGAACTTGTG ACAATCTGGT GAATGGATAC 
AGGTGTACTT GCAAGAAGGG CTTTAAAGGC TATAACTGCC AGGTGAATAT TGATGAATGT 
GCCTCAAATC CATGCCTGAA CCAAGGAACC TGCTTTGATG ACATAAGTGG CTACACTTGC 
CACTGTGTGC TGCCATACAC AGGCAAGAAT TGTCAGACAG TATTGGCTCC CTGTTCCCCA 
AACCCTTGTG AGAATGCTGC TGTTTGCAAA GAGTCACCAA ATTTTGAGAG TTATACTTGC 
TTGTGTGCTC CTGGCTGGCA AGGTCAGCGG TGTACCATTG ACATTGACGA GTGTATCTCC 
AAGCCCTGCA TGAACCATGG TCTCTGCCAT AACACCCAGG GCAGCTACAT GTGTGAATGT 
CCACCAGGCT TCAGTGGTAT GGACTGTGAG GAGGACATTG ATGACTGCCT TGCCAATCCT 
TGCCAGAATG GAGGTTCCTG TGTGGATGGA GTGAATACTT TCTCCTGCCT CTGCCTTCCG 
GGTTTCACTG GGGATAAGTG CCAGACAGAC ATGAATGAGT GTCTGAGTGA ACCCTGTAAG 
AATGGAGGGA CCTGCTCTGA CTACGTCAAC AGTTACACTT GCAAGTGCCA GGCAGGATTT 
GATGGAGTCC ATTGTGAGAA CAACATCAAT GAGTGCACTG AGAGCTCCTG TTTCAATGGT 
GGCACATGTG TTGATGGGAT TAACTCCTTC TCTTGCTTGT GCCCTGTGGG TTTCACTGGA 
TCCTTCTGCC TCCATGAGAT CAATGAATGC AGCTCTCATC CATGCCTCAA TGAGGGAACG 
TGTGTTGATG GCCTGGGTAC CTACCGCTGC AGCTGCCCCC TGGGCTACAC TGGGAAAAAC 
TGTCAGACCC TGGTGAACCT CTGCAGTCGG TCTCCATGTA AAAACAAAGG TACTTGCGTT 
CAGAAAAAAG CAGAGTCCCA GTGCCTATGT CCATCTGGAT GGGCTGGTGC CTATTGTGAC 
GTGCCCAATG TCTCTTGTGA CATAGCAGCC TCCAGGAGAG GTGTGCTTGT TGAACACTTG 
TGCCAGCACT CAGGTGTCTG CATCAATGCT GGCAACACGC ATTACTGTCA GTGCCCCCTG 
GGCTATACTG GGAGCTACTG TGAGGAGCAA CTCGATGAGT GTGCGTCCAA CCCCTGCCAG 
CACGGGGCAA CATGCAGTGA CTTCATTGGT GGATACAGAT GTGAGTGTGT CCCAGGCTAT 
CAGGGTGTCA ACTGTGAGTA TGAAGTGGAT GAGTGCCAGA ATCAGCCCTG CCAGAATGGA 
GGCACCTGTA TTGACCTTGT GAACCATTTC AAGTGCTCTT GCCCACCAGG CACTCGGGGC 
CTACTCTGTG AAGAGAACAT TGATGACTGT GCCCGGGGTC CCCATTGCCT TAATGGTGGT 
CAGTGCATGG ATAGGATTGG AGGCTACAGT TGTCGCTGCT TGCCTGGCTT TGCTGGGGAG 
CGTTGTGAGG GAGACATCAA CGAGTGCCTC TCCAACCCCT GCAGCTCTGA GGGCAGCCTG 
GACTGTATAC AGCTCACCAA TGACTACCTG TGTGTTTGCC GTAGTGCCTT TACTGGCCGG 
CACTGTGAAA CCTTCGTCGA TGTGTGTCCC CAGATGCCCT GCCTGAATGG AGGGACTTGT 
GCTGTGGCCA GTAACATGCC TGATGGTTTC ATTTGCCGTT GTCCCCCGGG ATTTTCTGGG 
GCAAGGTGCC AGAGCAGCTG TGGACAAGTG AAATGTAGGA AAGGGGAGCA GTGTGTGCAC 
ACCGCCTCTG GACCCCGCTG CTTCTGCCCC AGTCCCCGGG ACTGCGAGTC AGGCTGTGCC 
AGTAGCCCCT GCCAGCACGG GGGCAGCTGC CACCCTCAGC GCCAGCCTCC TTATTACTCC 
TGCCAGTGTG CCCCACCATT CTCGGGTAGC CGCTGTGAAC TCTACACGGC ACCCCCCAGC 
ACCCCTCCTG CCACCTGTCT GAGCCAGTAT TGTGCCGACA AAGCTCGGGA TGGCGTCTGT 
GATGAGGCCT GCAACAGCCA TGCCTGCCAG TGGGATGGGG GTGACTGTTC TCTCACCATG 
GAGAACCCCT GGGCCAACTG CTCCTCCCCA CTTCCCTGCT GGGATTATAT CAACAACCAG 
TGTGATGAGC TGTGCAACAC GGTCGAGTGC CTGTTTGACA ACTTTGAATG CCAGGGGAAC 
AGCAAGACAT GCAAGTATGA CAAATACTGT GCAGACCACT TCAAAGACAA CCACTGTGAC 
CAGGGGTGCA ACAGTGAGGA GTGTGGTTGG GATGGGCTGG ACTGTGCTGC TGACCAACCT 
GAGAACCTGG CAGAAGGTAC CCTGGTTATT GTGGTATTGA TGCCACCTGA ACAACTGCTC 
CAGGATGCTC GCAGCTTCTT GCGGGCACTG GGTACCCTGC TGCACACCAA CCTGCGCATT 
AAGCGGGACT CCCAGGGGGA ACTCATGGTG TACCCCTATT ATGGTGAGAA GTCAGCTGCT 
ATGAAGAAAC AGAGGATGAC ACGCAGGTCC CTTCCTGGTG AACAAGAACA GGAGGTGGCT 
GGCTCTAAAG TCTTTCTGGA AATTGACAAC CGCCAGTGTG TTCAAGACTC AGACCACTGC 
TTCAAGAACA CGGATGCAGC AGCAGCTCTC CTGGCCTCTC ACGCCATACA GGGGACCCTG 
TCATACCCTC TTGTGTCTGT CGTCAGTGAA TCCCTGACTC CAGAACGCAC TCAGCTCCTC 
TATCTCCTTG CTGTTGCTGT TGTCATCATT CTGTTTATTA TTCTGCTGGG GGTAATCATG 
GCAAAACGAA AGCGTAAGCA TGGCTCTCTC TGGCTGCCTG AAGGTTTCAC TCTTCGCCGA 
GATGCAAGCA ATCACAAGCG TCGTGAGCCA GTGGGACAGG ATGCTGTGGG GCTAAAAAAT 
CTCTCAGTGC AAGTCTCAGA AGCTAACCTA ATTGGTTCTG GAACAAGTGA ACACTGGGTC 
GATGATGAAG GGCCCCAGCC AAAGAAAGTA AAGGCTGAAG ATGAGGCCTT ACTCTCAGAA 
GAAGATGACC CCATTGATCG ACGGCCATGG ACACAGCAGC ACCTTGAAGC CGCAGACATC 
CGTAGGACAC CATCGCTGGC TCTCACCCCT CCTCAGGCAG AGCAGGAGGT GGATGTGTTA 
GATGTGAATG TCCGTGGCCC AGATGGCTGC ACCCCATTGA TGTTGGCTTC TCTCCGAGGA 
GGCAGCTCAG ATTTGAGTGA TGAAGATGAA GATGCAGAGG ACTCTTCTGC TAACATCATC 
ACAGACTTGG TCTACCAGGG TGCCAGCCTC CAGGCCCAGA CAGACCGGAC TGGTGAGATG 
GCCCTGCACC TTGCAGCCCG CTACTCACGG GCTGATGCTG CCAAGCGTCT CCTGGATGCA 
GGTGCAGATG CCAATGCCCA GGACAACATG GGCCGCTGCC CACTCCATGC TGCAGTGGCA 
GCTGATGCCC AAGGTGTCTT CCAGATTCTG ATTCGCAACC GAGTAACTGA TCTAGATGCC 
AGGATGAATG ATGGTACTAC ACCCCTGATC CTGGCTGCCC GCCTGGCTGT GGAGGGAATG 
GTGGCAGAAC TGATCAACTG CCAAGCGGAT GTGAATGCAG TGGATGACCA TGGAAAATCT 
GCTCTTCACT GGGCAGCTGC TGTCAATAAT GTGGAGGCAA CTCTTTTGTT GTTGAAAAAT 
GGAGCCAACC GAGACATGCA GGACAACAAG GAAGAGACAC CTCTGTTTCT TGCTGCCCGG 
GAGGGGAGCT ATGAAGCAGC CAAGATCCTG TTAGACCATT TTGCCAATCG AGACATCACA 
GACCATATGG ATCGTCTTCC CCGGGATGTG GCTCGGGATC GCATGCACCA TGACATTGTG 
CGCCTTCTGG ATGAATACAA TGTGACCCCA AGCCCTCCAG GCACCGTGTT GACTTCTGCT 
CTCTCACCTG TCATCTGTGG GCCCAACAGA TCTTTCCTCA GCCTGAAGCA CACCCCAATG 
GGCAAGAAGT CTAGACGGCC CAGTGCCAAG AGTACCATGC CTACTAGCCT CCCTAACCTT 
GCCAAGGAGG CAAAGGATGC CAAGGGTAGT AGGAGGAAGA AGTCTCTGAG TGAGAAGGTC 
CAACTGTCTG AGAGTTCAGT AACTTTATCC CCTGTTGATT CCCTAGAATC TCCTCACACG 
TATGTTTCCG ACACCACATC CTCTCCAATG ATTACATCCC CTGGGATCTT ACAGGCCTCA 
CCCAACCCTA TGTTGGCCAC TGCCGCCCCT CCTGCCCCAG TCCATGCCCA GCATGCACTA 
TCTTTTTCTA ACCTTCATGA AATGCAGCCT TTGGCACATG GGGCCAGCAC TGTGCTTCCC 
TCAGTGAGCC AGTTGCTATC CCACCACCAC ATTGTGTCTC CAGGCAGTGG CAGTGCTGGA 
AGCTTGAGTA GGCTCCATCC AGTCCCAGTC CCAGCAGATT GGATGAACCG CATGGAGGTG 
AATGAGACCC AGTACAATGA GATGTTTGGT ATGGTCCTGG CTCCAGCTGA GGGCACCCAT 
CCTGGCATAG CTCCCCAGAG CAGGCCACCT GAAGGGAAGC ACATAACCAC CCCTCGGGAG 
CCCTTGCCCC CCATTGTGAC TTTCCAGCTC ATCCCTAAAG GCAGTATTGC CCAACCAGCG 
GGGGCTCCCC AGCCTCAGTC CACCTGCCCT CCAGCTGTTG CGGGCCCCCT GCCCACCATG 
TACCAGATTC CAGAAATGGC CCGTTTGCCC AGTGTGGCTT TCCCCACTGC CATGATGCCC 
CAGCAGGACG GGCAGGTAGC TCAGACCATT CTCCCAGCCT ATCATCCTTT CCCAGCCTCT 
GTGGGCAAGT ACCCCACACC CCCTTCACAG CACAGTTATG CTTCCTCAAA TGCTGCTGAG 
CGAACACCCA GTCACAGTGG TCACCTCCAG GGTGAGCATC CCTACCTGAC ACCATCCCCA 
GAGTCTCCTG ACCAGTGGTC AAGTTCATCA CCCCACTCTG CTTCTGACTG GTCAGATGTG 
ACCACCAGCC CTACCCCTGG GGGTGCTGGA GGAGGTCAGC GGGGACCTGG GACACACATG 
TCTGAGCCAC CACACAACAA CATGCAGGTT TATGCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NOTCH2 ( XM_016924527.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016780016.1 Pan troglodytes notch 2 (NOTCH2), transcript variant X1, mRNA.

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CloneID	OPc59960
Clone ID Related Accession (Same CDS sequence)	XM_016924527.1
Accession Version	XM_016924527.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7839bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	neurogenic locus notch homolog protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006468.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGACGG GGCCAGTGAC TCGTAGTAGA TCCCTCCGCG CGGAGCTCGG GCCGGCGCTT 
CTTCCTGCGG GAAACCCCTG GGTGCCCAAG GCGGCGGGGC CGAGGCCGCG GCGACAGTGG 
GGCGGGGCTT GCGGTGGGAG GAGGCGGCTG AGGCGGAAGG ACACACGAGG CTGCTTCGCT 
GCACACCCGA GAAAGTTTCA GCCAAACTTC GGGCGGCGGC TGAGGCGGCG GCCGAGGAGC 
GGCGGACTCG GGGGCCGGGG AGTCCAGGCA TTTGCGCCTG GGCTTCGGAG CGTAGCGCCA 
GGGCCTGAGC CTTTGAAGCA GGAGGAGGGG AGGAGAGAGT GGGGCTCCTC TATCGGGACC 
CCCTCCCCAT GTGGATCTGC CCAGGCGGCG GCGGCGGCGG CGGCGGAGGA GGCGACCGAG 
AAGATGCCCG CCCTGCGCCC CGCTCTGCTG TGGGCGCTGC TGGCGCTCTG GCTGTGCTGC 
GCGGCCCCCG CGCATGCATT GCAGTGTCGA GATGGCTATG AACCCTGTGT AAATGAAGGA 
ATGTGTGTTA CCTACCACAA TGGCACAGGA TACTGCAAAT GTCCAGAAGG CTTCTTGGGG 
GAATATTGTC AACATCGAGA CCCCTGTGAG AAGAACCGCT GCCAGAATGG TGGGACTTGT 
GTGGCCCAGG CCATGCTGGG GAAAGCCATG TGCCGATGTG CCTCAGGGTT TACAGGAGAG 
GACTGCCAGT ACTCGACATC TCATCCATGC TTTGTGTCTC GACCCTGCCT GAATGGCGGC 
ACATGCCATA TGCTCAGCCG GGATACCTAT GAGTGCACCT GTCAAGTCGG GTTTACAGGT 
AAGGAGTGCC AATGGACCGA TGCCTGCCTG TCTCATCCCT GTGCAAATGG AAGTACCTGT 
ACCACTGTGG CCAACCAGTT CTCCTGCAAA TGCCTCACAG GCTTCACAGG GCAGAAGTGT 
GAGACTGATG TCAATGAGTG TGACATTCCA GGACACTGCC AGCATGGTGG CACCTGCCTC 
AACCTGCCTG GTTCCTACCA GTGCCAGTGC CCTCAGGGCT TCACAGGCCA GTACTGTGAC 
AGCCTGTATG TGCCCTGTGC ACCCTCGCCT TGTGTCAATG GAGGCACCTG TCGGCAGACT 
GGTGACTTCA CTTTTGAGTG CAACTGCCTT CCAGGTTTTG AAGGGAGCAC CTGTGAGAGG 
AATATTGATG ACTGCCCTAA CCACAGGTGT CAGAATGGAG GGGTTTGTGT GGATGGGGTC 
AACACTTACA ACTGCCGCTG TCCCCCACAA TGGACAGGAC AGTTCTGCAC AGAGGATGTG 
GATGAATGCC TGCTGCAGCC CAATGCCTGT CAAAATGGGG GCACCTGTGC CAACCGCAAT 
GGAGGCTATG GCTGTGTATG TGTCAACGGC TGGAGTGGAG ATGACTGCAG TGAGAACATT 
GATGATTGTG CCTTCGCCTC CTGTACTCCA GGCTCCACCT GCATCGACCG TGTGGCCTCC 
TTCTCTTGCA TGTGCCCAGA GGGGAAGGCA GGTCTCCTGT GTCATCTGGA TGATGCATGC 
ATCAGCAATC CTTGCCACAA GGGGGCACTG TGTGACACCA ACCCCCTAAA TGGGCAATAT 
ATTTGCACCT GCCCACAAGG CTACAAAGGG GCTGACTGCA CAGAAGATGT GGATGAATGT 
TCCATGGCCA ATAGCAATCC TTGTGAGCAT GCAGGAAAAT GTGTGAACAC GGATGGCGCC 
TTCCACTGTG AGTGTCTGAA GGGTTATGCA GGACCTCGTT GTGAGATGGA CATCAATGAG 
TGCCATTCAG ACCCCTGCCA GAATGATGCT ACCTGTCTGG ATAAGATTGG AGGCTTCACA 
TGTCTGTGCA TGCCAGGTTT CAAAGGTGTG CATTGTGAAT TAGAAATAAA TGAATGTCAG 
AGCAACCCTT GTGTGAACAA TGGGCAGTGT GTGGATAAAG TCAATCGTTT CCAGTGCCTG 
TGTCCTCCTG GTTTCACTGG GCCAGTTTGC CAGATTGATA TTGATGACTG TTCCAGTACT 
CCGTGTCTGA ATGGGGCAAA GTGTATCGAT CACCCGAATG GCTATGAATG CCAGTGTGCC 
ACAGGTTTCA CTGGTGTGTT GTGTGAGGAG AACATTGACA ACTGTGACCC CGATCCTTGC 
CACCATGGTC AGTGTCAGGA TGGTATTGAT TCCTACACCT GCATCTGCAA TCCCGGGTAC 
ATGGGCGCCA TCTGCAGTGA CCAGATTGAT GAATGTTACA GCAGCCCTTG CCTGAACGAT 
GGTCGCTGCA TTGACCTGGT CAATGGCTAC CAGTGCAACT GCCAGCCGGG CACGTCAGGG 
GTTAATTGTG AAATTAATTT TGATGACTGT GCAAGTAACC CTTGTATCCA TGGAAACTGT 
ATGGATGGCA TTAATCGCTA CAGTTGTGTC TGCTCACCAG GATTCACAGG GCAGAGATGT 
AACATTGACA TTGATGAGTG TGCCTCCAAT CCCTGTCGCA AGGGTGCAAC ATGTATCAAC 
GGTGTGAATG GTTTCCGCTG TATATGCCCC GAGGGACCCC ATCACCCCAG CTGCTACTCA 
CAGGTGAACG AATGCCTGAG CAATCCCTGC ATCCATGGAA ACTGTACTGG AGGTCTCAGT 
GGATATAAGT GTCTCTGTGA TGCAGGCTGG GTTGGCATCA ACTGTGAAGT GGACAAAAAT 
GAATGCCTTT CGAATCCATG CCAGAATGGA GGAACTTGTG ACAATCTGGT GAATGGATAC 
AGGTGTACTT GCAAGAAGGG CTTTAAAGGC TATAACTGCC AGGTGAATAT TGATGAATGT 
GCCTCAAATC CATGCCTGAA CCAAGGAACC TGCTTTGATG ACATAAGTGG CTACACTTGC 
CACTGTGTGC TGCCATACAC AGGCAAGAAT TGTCAGACAG TATTGGCTCC CTGTTCCCCA 
AACCCTTGTG AGAATGCTGC TGTTTGCAAA GAGTCACCAA ATTTTGAGAG TTATACTTGC 
TTGTGTGCTC CTGGCTGGCA AGGTCAGCGG TGTACCATTG ACATTGACGA GTGTATCTCC 
AAGCCCTGCA TGAACCATGG TCTCTGCCAT AACACCCAGG GCAGCTACAT GTGTGAATGT 
CCACCAGGCT TCAGTGGTAT GGACTGTGAG GAGGACATTG ATGACTGCCT TGCCAATCCT 
TGCCAGAATG GAGGTTCCTG TGTGGATGGA GTGAATACTT TCTCCTGCCT CTGCCTTCCG 
GGTTTCACTG GGGATAAGTG CCAGACAGAC ATGAATGAGT GTCTGAGTGA ACCCTGTAAG 
AATGGAGGGA CCTGCTCTGA CTACGTCAAC AGTTACACTT GCAAGTGCCA GGCAGGATTT 
GATGGAGTCC ATTGTGAGAA CAACATCAAT GAGTGCACTG AGAGCTCCTG TTTCAATGGT 
GGCACATGTG TTGATGGGAT TAACTCCTTC TCTTGCTTGT GCCCTGTGGG TTTCACTGGA 
TCCTTCTGCC TCCATGAGAT CAATGAATGC AGCTCTCATC CATGCCTCAA TGAGGGAACG 
TGTGTTGATG GCCTGGGTAC CTACCGCTGC AGCTGCCCCC TGGGCTACAC TGGGAAAAAC 
TGTCAGACCC TGGTGAACCT CTGCAGTCGG TCTCCATGTA AAAACAAAGG TACTTGCGTT 
CAGAAAAAAG CAGAGTCCCA GTGCCTATGT CCATCTGGAT GGGCTGGTGC CTATTGTGAC 
GTGCCCAATG TCTCTTGTGA CATAGCAGCC TCCAGGAGAG GTGTGCTTGT TGAACACTTG 
TGCCAGCACT CAGGTGTCTG CATCAATGCT GGCAACACGC ATTACTGTCA GTGCCCCCTG 
GGCTATACTG GGAGCTACTG TGAGGAGCAA CTCGATGAGT GTGCGTCCAA CCCCTGCCAG 
CACGGGGCAA CATGCAGTGA CTTCATTGGT GGATACAGAT GTGAGTGTGT CCCAGGCTAT 
CAGGGTGTCA ACTGTGAGTA TGAAGTGGAT GAGTGCCAGA ATCAGCCCTG CCAGAATGGA 
GGCACCTGTA TTGACCTTGT GAACCATTTC AAGTGCTCTT GCCCACCAGG CACTCGGGGC 
CTACTCTGTG AAGAGAACAT TGATGACTGT GCCCGGGGTC CCCATTGCCT TAATGGTGGT 
CAGTGCATGG ATAGGATTGG AGGCTACAGT TGTCGCTGCT TGCCTGGCTT TGCTGGGGAG 
CGTTGTGAGG GAGACATCAA CGAGTGCCTC TCCAACCCCT GCAGCTCTGA GGGCAGCCTG 
GACTGTATAC AGCTCACCAA TGACTACCTG TGTGTTTGCC GTAGTGCCTT TACTGGCCGG 
CACTGTGAAA CCTTCGTCGA TGTGTGTCCC CAGATGCCCT GCCTGAATGG AGGGACTTGT 
GCTGTGGCCA GTAACATGCC TGATGGTTTC ATTTGCCGTT GTCCCCCGGG ATTTTCTGGG 
GCAAGGTGCC AGAGCAGCTG TGGACAAGTG AAATGTAGGA AAGGGGAGCA GTGTGTGCAC 
ACCGCCTCTG GACCCCGCTG CTTCTGCCCC AGTCCCCGGG ACTGCGAGTC AGGCTGTGCC 
AGTAGCCCCT GCCAGCACGG GGGCAGCTGC CACCCTCAGC GCCAGCCTCC TTATTACTCC 
TGCCAGTGTG CCCCACCATT CTCGGGTAGC CGCTGTGAAC TCTACACGGC ACCCCCCAGC 
ACCCCTCCTG CCACCTGTCT GAGCCAGTAT TGTGCCGACA AAGCTCGGGA TGGCGTCTGT 
GATGAGGCCT GCAACAGCCA TGCCTGCCAG TGGGATGGGG GTGACTGTTC TCTCACCATG 
GAGAACCCCT GGGCCAACTG CTCCTCCCCA CTTCCCTGCT GGGATTATAT CAACAACCAG 
TGTGATGAGC TGTGCAACAC GGTCGAGTGC CTGTTTGACA ACTTTGAATG CCAGGGGAAC 
AGCAAGACAT GCAAGTATGA CAAATACTGT GCAGACCACT TCAAAGACAA CCACTGTGAC 
CAGGGGTGCA ACAGTGAGGA GTGTGGTTGG GATGGGCTGG ACTGTGCTGC TGACCAACCT 
GAGAACCTGG CAGAAGGTAC CCTGGTTATT GTGGTATTGA TGCCACCTGA ACAACTGCTC 
CAGGATGCTC GCAGCTTCTT GCGGGCACTG GGTACCCTGC TGCACACCAA CCTGCGCATT 
AAGCGGGACT CCCAGGGGGA ACTCATGGTG TACCCCTATT ATGGTGAGAA GTCAGCTGCT 
ATGAAGAAAC AGAGGATGAC ACGCAGGTCC CTTCCTGGTG AACAAGAACA GGAGGTGGCT 
GGCTCTAAAG TCTTTCTGGA AATTGACAAC CGCCAGTGTG TTCAAGACTC AGACCACTGC 
TTCAAGAACA CGGATGCAGC AGCAGCTCTC CTGGCCTCTC ACGCCATACA GGGGACCCTG 
TCATACCCTC TTGTGTCTGT CGTCAGTGAA TCCCTGACTC CAGAACGCAC TCAGCTCCTC 
TATCTCCTTG CTGTTGCTGT TGTCATCATT CTGTTTATTA TTCTGCTGGG GGTAATCATG 
GCAAAACGAA AGCGTAAGCA TGGCTCTCTC TGGCTGCCTG AAGGTTTCAC TCTTCGCCGA 
GATGCAAGCA ATCACAAGCG TCGTGAGCCA GTGGGACAGG ATGCTGTGGG GCTAAAAAAT 
CTCTCAGTGC AAGTCTCAGA AGCTAACCTA ATTGGTTCTG GAACAAGTGA ACACTGGGTC 
GATGATGAAG GGCCCCAGCC AAAGAAAGTA AAGGCTGAAG ATGAGGCCTT ACTCTCAGAA 
GAAGATGACC CCATTGATCG ACGGCCATGG ACACAGCAGC ACCTTGAAGC CGCAGACATC 
CGTAGGACAC CATCGCTGGC TCTCACCCCT CCTCAGGCAG AGCAGGAGGT GGATGTGTTA 
GATGTGAATG TCCGTGGCCC AGATGGCTGC ACCCCATTGA TGTTGGCTTC TCTCCGAGGA 
GGCAGCTCAG ATTTGAGTGA TGAAGATGAA GATGCAGAGG ACTCTTCTGC TAACATCATC 
ACAGACTTGG TCTACCAGGG TGCCAGCCTC CAGGCCCAGA CAGACCGGAC TGGTGAGATG 
GCCCTGCACC TTGCAGCCCG CTACTCACGG GCTGATGCTG CCAAGCGTCT CCTGGATGCA 
GGTGCAGATG CCAATGCCCA GGACAACATG GGCCGCTGCC CACTCCATGC TGCAGTGGCA 
GCTGATGCCC AAGGTGTCTT CCAGATTCTG ATTCGCAACC GAGTAACTGA TCTAGATGCC 
AGGATGAATG ATGGTACTAC ACCCCTGATC CTGGCTGCCC GCCTGGCTGT GGAGGGAATG 
GTGGCAGAAC TGATCAACTG CCAAGCGGAT GTGAATGCAG TGGATGACCA TGGAAAATCT 
GCTCTTCACT GGGCAGCTGC TGTCAATAAT GTGGAGGCAA CTCTTTTGTT GTTGAAAAAT 
GGAGCCAACC GAGACATGCA GGACAACAAG GAAGAGACAC CTCTGTTTCT TGCTGCCCGG 
GAGGGGAGCT ATGAAGCAGC CAAGATCCTG TTAGACCATT TTGCCAATCG AGACATCACA 
GACCATATGG ATCGTCTTCC CCGGGATGTG GCTCGGGATC GCATGCACCA TGACATTGTG 
CGCCTTCTGG ATGAATACAA TGTGACCCCA AGCCCTCCAG GCACCGTGTT GACTTCTGCT 
CTCTCACCTG TCATCTGTGG GCCCAACAGA TCTTTCCTCA GCCTGAAGCA CACCCCAATG 
GGCAAGAAGT CTAGACGGCC CAGTGCCAAG AGTACCATGC CTACTAGCCT CCCTAACCTT 
GCCAAGGAGG CAAAGGATGC CAAGGGTAGT AGGAGGAAGA AGTCTCTGAG TGAGAAGGTC 
CAACTGTCTG AGAGTTCAGT AACTTTATCC CCTGTTGATT CCCTAGAATC TCCTCACACG 
TATGTTTCCG ACACCACATC CTCTCCAATG ATTACATCCC CTGGGATCTT ACAGGCCTCA 
CCCAACCCTA TGTTGGCCAC TGCCGCCCCT CCTGCCCCAG TCCATGCCCA GCATGCACTA 
TCTTTTTCTA ACCTTCATGA AATGCAGCCT TTGGCACATG GGGCCAGCAC TGTGCTTCCC 
TCAGTGAGCC AGTTGCTATC CCACCACCAC ATTGTGTCTC CAGGCAGTGG CAGTGCTGGA 
AGCTTGAGTA GGCTCCATCC AGTCCCAGTC CCAGCAGATT GGATGAACCG CATGGAGGTG 
AATGAGACCC AGTACAATGA GATGTTTGGT ATGGTCCTGG CTCCAGCTGA GGGCACCCAT 
CCTGGCATAG CTCCCCAGAG CAGGCCACCT GAAGGGAAGC ACATAACCAC CCCTCGGGAG 
CCCTTGCCCC CCATTGTGAC TTTCCAGCTC ATCCCTAAAG GCAGTATTGC CCAACCAGCG 
GGGGCTCCCC AGCCTCAGTC CACCTGCCCT CCAGCTGTTG CGGGCCCCCT GCCCACCATG 
TACCAGATTC CAGAAATGGC CCGTTTGCCC AGTGTGGCTT TCCCCACTGC CATGATGCCC 
CAGCAGGACG GGCAGGTAGC TCAGACCATT CTCCCAGCCT ATCATCCTTT CCCAGCCTCT 
GTGGGCAAGT ACCCCACACC CCCTTCACAG CACAGTTATG CTTCCTCAAA TGCTGCTGAG 
CGAACACCCA GTCACAGTGG TCACCTCCAG GGTGAGCATC CCTACCTGAC ACCATCCCCA 
GAGTCTCCTG ACCAGTGGTC AAGTTCATCA CCCCACTCTG CTTCTGACTG GTCAGATGTG 
ACCACCAGCC CTACCCCTGG GGGTGCTGGA GGAGGTCAGC GGGGACCTGG GACACACATG 
TCTGAGCCAC CACACAACAA CATGCAGGTT TATGCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016780016.1
CDS	878..8716
Translation	MRTGPVTRSRSLRAELGPALLPAGNPWVPKAAGPRPRRQWGGACGGRRRLRRKDTRGCFAAHPRKFQPNFGRRLRRRPRSGGLGGRGVQAFAPGLRSVAPGPEPLKQEEGRREWGSSIGTPSPCGSAQAAAAAAAEEATEKMPALRPALLWALLALWLCCAAPAHALQCRDGYEPCVNEGMCVTYHNGTGYCKCPEGFLGEYCQHRDPCEKNRCQNGGTCVAQAMLGKAMCRCASGFTGEDCQYSTSHPCFVSRPCLNGGTCHMLSRDTYECTCQVGFTGKECQWTDACLSHPCANGSTCTTVANQFSCKCLTGFTGQKCETDVNECDIPGHCQHGGTCLNLPGSYQCQCPQGFTGQYCDSLYVPCAPSPCVNGGTCRQTGDFTFECNCLPGFEGSTCERNIDDCPNHRCQNGGVCVDGVNTYNCRCPPQWTGQFCTEDVDECLLQPNACQNGGTCANRNGGYGCVCVNGWSGDDCSENIDDCAFASCTPGSTCIDRVASFSCMCPEGKAGLLCHLDDACISNPCHKGALCDTNPLNGQYICTCPQGYKGADCTEDVDECSMANSNPCEHAGKCVNTDGAFHCECLKGYAGPRCEMDINECHSDPCQNDATCLDKIGGFTCLCMPGFKGVHCELEINECQSNPCVNNGQCVDKVNRFQCLCPPGFTGPVCQIDIDDCSSTPCLNGAKCIDHPNGYECQCATGFTGVLCEENIDNCDPDPCHHGQCQDGIDSYTCICNPGYMGAICSDQIDECYSSPCLNDGRCIDLVNGYQCNCQPGTSGVNCEINFDDCASNPCIHGNCMDGINRYSCVCSPGFTGQRCNIDIDECASNPCRKGATCINGVNGFRCICPEGPHHPSCYSQVNECLSNPCIHGNCTGGLSGYKCLCDAGWVGINCEVDKNECLSNPCQNGGTCDNLVNGYRCTCKKGFKGYNCQVNIDECASNPCLNQGTCFDDISGYTCHCVLPYTGKNCQTVLAPCSPNPCENAAVCKESPNFESYTCLCAPGWQGQRCTIDIDECISKPCMNHGLCHNTQGSYMCECPPGFSGMDCEEDIDDCLANPCQNGGSCVDGVNTFSCLCLPGFTGDKCQTDMNECLSEPCKNGGTCSDYVNSYTCKCQAGFDGVHCENNINECTESSCFNGGTCVDGINSFSCLCPVGFTGSFCLHEINECSSHPCLNEGTCVDGLGTYRCSCPLGYTGKNCQTLVNLCSRSPCKNKGTCVQKKAESQCLCPSGWAGAYCDVPNVSCDIAASRRGVLVEHLCQHSGVCINAGNTHYCQCPLGYTGSYCEEQLDECASNPCQHGATCSDFIGGYRCECVPGYQGVNCEYEVDECQNQPCQNGGTCIDLVNHFKCSCPPGTRGLLCEENIDDCARGPHCLNGGQCMDRIGGYSCRCLPGFAGERCEGDINECLSNPCSSEGSLDCIQLTNDYLCVCRSAFTGRHCETFVDVCPQMPCLNGGTCAVASNMPDGFICRCPPGFSGARCQSSCGQVKCRKGEQCVHTASGPRCFCPSPRDCESGCASSPCQHGGSCHPQRQPPYYSCQCAPPFSGSRCELYTAPPSTPPATCLSQYCADKARDGVCDEACNSHACQWDGGDCSLTMENPWANCSSPLPCWDYINNQCDELCNTVECLFDNFECQGNSKTCKYDKYCADHFKDNHCDQGCNSEECGWDGLDCAADQPENLAEGTLVIVVLMPPEQLLQDARSFLRALGTLLHTNLRIKRDSQGELMVYPYYGEKSAAMKKQRMTRRSLPGEQEQEVAGSKVFLEIDNRQCVQDSDHCFKNTDAAAALLASHAIQGTLSYPLVSVVSESLTPERTQLLYLLAVAVVIILFIILLGVIMAKRKRKHGSLWLPEGFTLRRDASNHKRREPVGQDAVGLKNLSVQVSEANLIGSGTSEHWVDDEGPQPKKVKAEDEALLSEEDDPIDRRPWTQQHLEAADIRRTPSLALTPPQAEQEVDVLDVNVRGPDGCTPLMLASLRGGSSDLSDEDEDAEDSSANIITDLVYQGASLQAQTDRTGEMALHLAARYSRADAAKRLLDAGADANAQDNMGRCPLHAAVAADAQGVFQILIRNRVTDLDARMNDGTTPLILAARLAVEGMVAELINCQADVNAVDDHGKSALHWAAAVNNVEATLLLLKNGANRDMQDNKEETPLFLAAREGSYEAAKILLDHFANRDITDHMDRLPRDVARDRMHHDIVRLLDEYNVTPSPPGTVLTSALSPVICGPNRSFLSLKHTPMGKKSRRPSAKSTMPTSLPNLAKEAKDAKGSRRKKSLSEKVQLSESSVTLSPVDSLESPHTYVSDTTSSPMITSPGILQASPNPMLATAAPPAPVHAQHALSFSNLHEMQPLAHGASTVLPSVSQLLSHHHIVSPGSGSAGSLSRLHPVPVPADWMNRMEVNETQYNEMFGMVLAPAEGTHPGIAPQSRPPEGKHITTPREPLPPIVTFQLIPKGSIAQPAGAPQPQSTCPPAVAGPLPTMYQIPEMARLPSVAFPTAMMPQQDGQVAQTILPAYHPFPASVGKYPTPPSQHSYASSNAAERTPSHSGHLQGEHPYLTPSPESPDQWSSSSPHSASDWSDVTTSPTPGGAGGGQRGPGTHMSEPPHNNMQVYA

Target ORF information:

RefSeq Version	XM_016924527.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes notch 2 (NOTCH2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016924527.1

ORF Insert Sequence:

ATGCGGACGG GGCCAGTGAC TCGTAGTAGA TCCCTCCGCG CGGAGCTCGG GCCGGCGCTT 
CTTCCTGCGG GAAACCCCTG GGTGCCCAAG GCGGCGGGGC CGAGGCCGCG GCGACAGTGG 
GGCGGGGCTT GCGGTGGGAG GAGGCGGCTG AGGCGGAAGG ACACACGAGG CTGCTTCGCT 
GCACACCCGA GAAAGTTTCA GCCAAACTTC GGGCGGCGGC TGAGGCGGCG GCCGAGGAGC 
GGCGGACTCG GGGGCCGGGG AGTCCAGGCA TTTGCGCCTG GGCTTCGGAG CGTAGCGCCA 
GGGCCTGAGC CTTTGAAGCA GGAGGAGGGG AGGAGAGAGT GGGGCTCCTC TATCGGGACC 
CCCTCCCCAT GTGGATCTGC CCAGGCGGCG GCGGCGGCGG CGGCGGAGGA GGCGACCGAG 
AAGATGCCCG CCCTGCGCCC CGCTCTGCTG TGGGCGCTGC TGGCGCTCTG GCTGTGCTGC 
GCGGCCCCCG CGCATGCATT GCAGTGTCGA GATGGCTATG AACCCTGTGT AAATGAAGGA 
ATGTGTGTTA CCTACCACAA TGGCACAGGA TACTGCAAAT GTCCAGAAGG CTTCTTGGGG 
GAATATTGTC AACATCGAGA CCCCTGTGAG AAGAACCGCT GCCAGAATGG TGGGACTTGT 
GTGGCCCAGG CCATGCTGGG GAAAGCCATG TGCCGATGTG CCTCAGGGTT TACAGGAGAG 
GACTGCCAGT ACTCGACATC TCATCCATGC TTTGTGTCTC GACCCTGCCT GAATGGCGGC 
ACATGCCATA TGCTCAGCCG GGATACCTAT GAGTGCACCT GTCAAGTCGG GTTTACAGGT 
AAGGAGTGCC AATGGACCGA TGCCTGCCTG TCTCATCCCT GTGCAAATGG AAGTACCTGT 
ACCACTGTGG CCAACCAGTT CTCCTGCAAA TGCCTCACAG GCTTCACAGG GCAGAAGTGT 
GAGACTGATG TCAATGAGTG TGACATTCCA GGACACTGCC AGCATGGTGG CACCTGCCTC 
AACCTGCCTG GTTCCTACCA GTGCCAGTGC CCTCAGGGCT TCACAGGCCA GTACTGTGAC 
AGCCTGTATG TGCCCTGTGC ACCCTCGCCT TGTGTCAATG GAGGCACCTG TCGGCAGACT 
GGTGACTTCA CTTTTGAGTG CAACTGCCTT CCAGGTTTTG AAGGGAGCAC CTGTGAGAGG 
AATATTGATG ACTGCCCTAA CCACAGGTGT CAGAATGGAG GGGTTTGTGT GGATGGGGTC 
AACACTTACA ACTGCCGCTG TCCCCCACAA TGGACAGGAC AGTTCTGCAC AGAGGATGTG 
GATGAATGCC TGCTGCAGCC CAATGCCTGT CAAAATGGGG GCACCTGTGC CAACCGCAAT 
GGAGGCTATG GCTGTGTATG TGTCAACGGC TGGAGTGGAG ATGACTGCAG TGAGAACATT 
GATGATTGTG CCTTCGCCTC CTGTACTCCA GGCTCCACCT GCATCGACCG TGTGGCCTCC 
TTCTCTTGCA TGTGCCCAGA GGGGAAGGCA GGTCTCCTGT GTCATCTGGA TGATGCATGC 
ATCAGCAATC CTTGCCACAA GGGGGCACTG TGTGACACCA ACCCCCTAAA TGGGCAATAT 
ATTTGCACCT GCCCACAAGG CTACAAAGGG GCTGACTGCA CAGAAGATGT GGATGAATGT 
TCCATGGCCA ATAGCAATCC TTGTGAGCAT GCAGGAAAAT GTGTGAACAC GGATGGCGCC 
TTCCACTGTG AGTGTCTGAA GGGTTATGCA GGACCTCGTT GTGAGATGGA CATCAATGAG 
TGCCATTCAG ACCCCTGCCA GAATGATGCT ACCTGTCTGG ATAAGATTGG AGGCTTCACA 
TGTCTGTGCA TGCCAGGTTT CAAAGGTGTG CATTGTGAAT TAGAAATAAA TGAATGTCAG 
AGCAACCCTT GTGTGAACAA TGGGCAGTGT GTGGATAAAG TCAATCGTTT CCAGTGCCTG 
TGTCCTCCTG GTTTCACTGG GCCAGTTTGC CAGATTGATA TTGATGACTG TTCCAGTACT 
CCGTGTCTGA ATGGGGCAAA GTGTATCGAT CACCCGAATG GCTATGAATG CCAGTGTGCC 
ACAGGTTTCA CTGGTGTGTT GTGTGAGGAG AACATTGACA ACTGTGACCC CGATCCTTGC 
CACCATGGTC AGTGTCAGGA TGGTATTGAT TCCTACACCT GCATCTGCAA TCCCGGGTAC 
ATGGGCGCCA TCTGCAGTGA CCAGATTGAT GAATGTTACA GCAGCCCTTG CCTGAACGAT 
GGTCGCTGCA TTGACCTGGT CAATGGCTAC CAGTGCAACT GCCAGCCGGG CACGTCAGGG 
GTTAATTGTG AAATTAATTT TGATGACTGT GCAAGTAACC CTTGTATCCA TGGAAACTGT 
ATGGATGGCA TTAATCGCTA CAGTTGTGTC TGCTCACCAG GATTCACAGG GCAGAGATGT 
AACATTGACA TTGATGAGTG TGCCTCCAAT CCCTGTCGCA AGGGTGCAAC ATGTATCAAC 
GGTGTGAATG GTTTCCGCTG TATATGCCCC GAGGGACCCC ATCACCCCAG CTGCTACTCA 
CAGGTGAACG AATGCCTGAG CAATCCCTGC ATCCATGGAA ACTGTACTGG AGGTCTCAGT 
GGATATAAGT GTCTCTGTGA TGCAGGCTGG GTTGGCATCA ACTGTGAAGT GGACAAAAAT 
GAATGCCTTT CGAATCCATG CCAGAATGGA GGAACTTGTG ACAATCTGGT GAATGGATAC 
AGGTGTACTT GCAAGAAGGG CTTTAAAGGC TATAACTGCC AGGTGAATAT TGATGAATGT 
GCCTCAAATC CATGCCTGAA CCAAGGAACC TGCTTTGATG ACATAAGTGG CTACACTTGC 
CACTGTGTGC TGCCATACAC AGGCAAGAAT TGTCAGACAG TATTGGCTCC CTGTTCCCCA 
AACCCTTGTG AGAATGCTGC TGTTTGCAAA GAGTCACCAA ATTTTGAGAG TTATACTTGC 
TTGTGTGCTC CTGGCTGGCA AGGTCAGCGG TGTACCATTG ACATTGACGA GTGTATCTCC 
AAGCCCTGCA TGAACCATGG TCTCTGCCAT AACACCCAGG GCAGCTACAT GTGTGAATGT 
CCACCAGGCT TCAGTGGTAT GGACTGTGAG GAGGACATTG ATGACTGCCT TGCCAATCCT 
TGCCAGAATG GAGGTTCCTG TGTGGATGGA GTGAATACTT TCTCCTGCCT CTGCCTTCCG 
GGTTTCACTG GGGATAAGTG CCAGACAGAC ATGAATGAGT GTCTGAGTGA ACCCTGTAAG 
AATGGAGGGA CCTGCTCTGA CTACGTCAAC AGTTACACTT GCAAGTGCCA GGCAGGATTT 
GATGGAGTCC ATTGTGAGAA CAACATCAAT GAGTGCACTG AGAGCTCCTG TTTCAATGGT 
GGCACATGTG TTGATGGGAT TAACTCCTTC TCTTGCTTGT GCCCTGTGGG TTTCACTGGA 
TCCTTCTGCC TCCATGAGAT CAATGAATGC AGCTCTCATC CATGCCTCAA TGAGGGAACG 
TGTGTTGATG GCCTGGGTAC CTACCGCTGC AGCTGCCCCC TGGGCTACAC TGGGAAAAAC 
TGTCAGACCC TGGTGAACCT CTGCAGTCGG TCTCCATGTA AAAACAAAGG TACTTGCGTT 
CAGAAAAAAG CAGAGTCCCA GTGCCTATGT CCATCTGGAT GGGCTGGTGC CTATTGTGAC 
GTGCCCAATG TCTCTTGTGA CATAGCAGCC TCCAGGAGAG GTGTGCTTGT TGAACACTTG 
TGCCAGCACT CAGGTGTCTG CATCAATGCT GGCAACACGC ATTACTGTCA GTGCCCCCTG 
GGCTATACTG GGAGCTACTG TGAGGAGCAA CTCGATGAGT GTGCGTCCAA CCCCTGCCAG 
CACGGGGCAA CATGCAGTGA CTTCATTGGT GGATACAGAT GTGAGTGTGT CCCAGGCTAT 
CAGGGTGTCA ACTGTGAGTA TGAAGTGGAT GAGTGCCAGA ATCAGCCCTG CCAGAATGGA 
GGCACCTGTA TTGACCTTGT GAACCATTTC AAGTGCTCTT GCCCACCAGG CACTCGGGGC 
CTACTCTGTG AAGAGAACAT TGATGACTGT GCCCGGGGTC CCCATTGCCT TAATGGTGGT 
CAGTGCATGG ATAGGATTGG AGGCTACAGT TGTCGCTGCT TGCCTGGCTT TGCTGGGGAG 
CGTTGTGAGG GAGACATCAA CGAGTGCCTC TCCAACCCCT GCAGCTCTGA GGGCAGCCTG 
GACTGTATAC AGCTCACCAA TGACTACCTG TGTGTTTGCC GTAGTGCCTT TACTGGCCGG 
CACTGTGAAA CCTTCGTCGA TGTGTGTCCC CAGATGCCCT GCCTGAATGG AGGGACTTGT 
GCTGTGGCCA GTAACATGCC TGATGGTTTC ATTTGCCGTT GTCCCCCGGG ATTTTCTGGG 
GCAAGGTGCC AGAGCAGCTG TGGACAAGTG AAATGTAGGA AAGGGGAGCA GTGTGTGCAC 
ACCGCCTCTG GACCCCGCTG CTTCTGCCCC AGTCCCCGGG ACTGCGAGTC AGGCTGTGCC 
AGTAGCCCCT GCCAGCACGG GGGCAGCTGC CACCCTCAGC GCCAGCCTCC TTATTACTCC 
TGCCAGTGTG CCCCACCATT CTCGGGTAGC CGCTGTGAAC TCTACACGGC ACCCCCCAGC 
ACCCCTCCTG CCACCTGTCT GAGCCAGTAT TGTGCCGACA AAGCTCGGGA TGGCGTCTGT 
GATGAGGCCT GCAACAGCCA TGCCTGCCAG TGGGATGGGG GTGACTGTTC TCTCACCATG 
GAGAACCCCT GGGCCAACTG CTCCTCCCCA CTTCCCTGCT GGGATTATAT CAACAACCAG 
TGTGATGAGC TGTGCAACAC GGTCGAGTGC CTGTTTGACA ACTTTGAATG CCAGGGGAAC 
AGCAAGACAT GCAAGTATGA CAAATACTGT GCAGACCACT TCAAAGACAA CCACTGTGAC 
CAGGGGTGCA ACAGTGAGGA GTGTGGTTGG GATGGGCTGG ACTGTGCTGC TGACCAACCT 
GAGAACCTGG CAGAAGGTAC CCTGGTTATT GTGGTATTGA TGCCACCTGA ACAACTGCTC 
CAGGATGCTC GCAGCTTCTT GCGGGCACTG GGTACCCTGC TGCACACCAA CCTGCGCATT 
AAGCGGGACT CCCAGGGGGA ACTCATGGTG TACCCCTATT ATGGTGAGAA GTCAGCTGCT 
ATGAAGAAAC AGAGGATGAC ACGCAGGTCC CTTCCTGGTG AACAAGAACA GGAGGTGGCT 
GGCTCTAAAG TCTTTCTGGA AATTGACAAC CGCCAGTGTG TTCAAGACTC AGACCACTGC 
TTCAAGAACA CGGATGCAGC AGCAGCTCTC CTGGCCTCTC ACGCCATACA GGGGACCCTG 
TCATACCCTC TTGTGTCTGT CGTCAGTGAA TCCCTGACTC CAGAACGCAC TCAGCTCCTC 
TATCTCCTTG CTGTTGCTGT TGTCATCATT CTGTTTATTA TTCTGCTGGG GGTAATCATG 
GCAAAACGAA AGCGTAAGCA TGGCTCTCTC TGGCTGCCTG AAGGTTTCAC TCTTCGCCGA 
GATGCAAGCA ATCACAAGCG TCGTGAGCCA GTGGGACAGG ATGCTGTGGG GCTAAAAAAT 
CTCTCAGTGC AAGTCTCAGA AGCTAACCTA ATTGGTTCTG GAACAAGTGA ACACTGGGTC 
GATGATGAAG GGCCCCAGCC AAAGAAAGTA AAGGCTGAAG ATGAGGCCTT ACTCTCAGAA 
GAAGATGACC CCATTGATCG ACGGCCATGG ACACAGCAGC ACCTTGAAGC CGCAGACATC 
CGTAGGACAC CATCGCTGGC TCTCACCCCT CCTCAGGCAG AGCAGGAGGT GGATGTGTTA 
GATGTGAATG TCCGTGGCCC AGATGGCTGC ACCCCATTGA TGTTGGCTTC TCTCCGAGGA 
GGCAGCTCAG ATTTGAGTGA TGAAGATGAA GATGCAGAGG ACTCTTCTGC TAACATCATC 
ACAGACTTGG TCTACCAGGG TGCCAGCCTC CAGGCCCAGA CAGACCGGAC TGGTGAGATG 
GCCCTGCACC TTGCAGCCCG CTACTCACGG GCTGATGCTG CCAAGCGTCT CCTGGATGCA 
GGTGCAGATG CCAATGCCCA GGACAACATG GGCCGCTGCC CACTCCATGC TGCAGTGGCA 
GCTGATGCCC AAGGTGTCTT CCAGATTCTG ATTCGCAACC GAGTAACTGA TCTAGATGCC 
AGGATGAATG ATGGTACTAC ACCCCTGATC CTGGCTGCCC GCCTGGCTGT GGAGGGAATG 
GTGGCAGAAC TGATCAACTG CCAAGCGGAT GTGAATGCAG TGGATGACCA TGGAAAATCT 
GCTCTTCACT GGGCAGCTGC TGTCAATAAT GTGGAGGCAA CTCTTTTGTT GTTGAAAAAT 
GGAGCCAACC GAGACATGCA GGACAACAAG GAAGAGACAC CTCTGTTTCT TGCTGCCCGG 
GAGGGGAGCT ATGAAGCAGC CAAGATCCTG TTAGACCATT TTGCCAATCG AGACATCACA 
GACCATATGG ATCGTCTTCC CCGGGATGTG GCTCGGGATC GCATGCACCA TGACATTGTG 
CGCCTTCTGG ATGAATACAA TGTGACCCCA AGCCCTCCAG GCACCGTGTT GACTTCTGCT 
CTCTCACCTG TCATCTGTGG GCCCAACAGA TCTTTCCTCA GCCTGAAGCA CACCCCAATG 
GGCAAGAAGT CTAGACGGCC CAGTGCCAAG AGTACCATGC CTACTAGCCT CCCTAACCTT 
GCCAAGGAGG CAAAGGATGC CAAGGGTAGT AGGAGGAAGA AGTCTCTGAG TGAGAAGGTC 
CAACTGTCTG AGAGTTCAGT AACTTTATCC CCTGTTGATT CCCTAGAATC TCCTCACACG 
TATGTTTCCG ACACCACATC CTCTCCAATG ATTACATCCC CTGGGATCTT ACAGGCCTCA 
CCCAACCCTA TGTTGGCCAC TGCCGCCCCT CCTGCCCCAG TCCATGCCCA GCATGCACTA 
TCTTTTTCTA ACCTTCATGA AATGCAGCCT TTGGCACATG GGGCCAGCAC TGTGCTTCCC 
TCAGTGAGCC AGTTGCTATC CCACCACCAC ATTGTGTCTC CAGGCAGTGG CAGTGCTGGA 
AGCTTGAGTA GGCTCCATCC AGTCCCAGTC CCAGCAGATT GGATGAACCG CATGGAGGTG 
AATGAGACCC AGTACAATGA GATGTTTGGT ATGGTCCTGG CTCCAGCTGA GGGCACCCAT 
CCTGGCATAG CTCCCCAGAG CAGGCCACCT GAAGGGAAGC ACATAACCAC CCCTCGGGAG 
CCCTTGCCCC CCATTGTGAC TTTCCAGCTC ATCCCTAAAG GCAGTATTGC CCAACCAGCG 
GGGGCTCCCC AGCCTCAGTC CACCTGCCCT CCAGCTGTTG CGGGCCCCCT GCCCACCATG 
TACCAGATTC CAGAAATGGC CCGTTTGCCC AGTGTGGCTT TCCCCACTGC CATGATGCCC 
CAGCAGGACG GGCAGGTAGC TCAGACCATT CTCCCAGCCT ATCATCCTTT CCCAGCCTCT 
GTGGGCAAGT ACCCCACACC CCCTTCACAG CACAGTTATG CTTCCTCAAA TGCTGCTGAG 
CGAACACCCA GTCACAGTGG TCACCTCCAG GGTGAGCATC CCTACCTGAC ACCATCCCCA 
GAGTCTCCTG ACCAGTGGTC AAGTTCATCA CCCCACTCTG CTTCTGACTG GTCAGATGTG 
ACCACCAGCC CTACCCCTGG GGGTGCTGGA GGAGGTCAGC GGGGACCTGG GACACACATG 
TCTGAGCCAC CACACAACAA CATGCAGGTT TATGCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NOTCH2 ( XM_016924554.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016780043.1 Pan troglodytes notch 2 (NOTCH2), transcript variant X5, mRNA.

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CloneID	OPc59963
Clone ID Related Accession (Same CDS sequence)	XM_016924554.1
Accession Version	XM_016924554.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1245bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	neurogenic locus notch homolog protein 2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006468.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGACGG GGCCAGTGAC TCGTAGTAGA TCCCTCCGCG CGGAGCTCGG GCCGGCGCTT 
CTTCCTGCGG GAAACCCCTG GGTGCCCAAG GCGGCGGGGC CGAGGCCGCG GCGACAGTGG 
GGCGGGGCTT GCGGTGGGAG GAGGCGGCTG AGGCGGAAGG ACACACGAGG CTGCTTCGCT 
GCACACCCGA GAAAGTTTCA GCCAAACTTC GGGCGGCGGC TGAGGCGGCG GCCGAGGAGC 
GGCGGACTCG GGGGCCGGGG AGTCCAGGCA TTTGCGCCTG GGCTTCGGAG CGTAGCGCCA 
GGGCCTGAGC CTTTGAAGCA GGAGGAGGGG AGGAGAGAGT GGGGCTCCTC TATCGGGACC 
CCCTCCCCAT GTGGATCTGC CCAGGCGGCG GCGGCGGCGG CGGCGGAGGA GGCGACCGAG 
AAGATGCCCG CCCTGCGCCC CGCTCTGCTG TGGGCGCTGC TGGCGCTCTG GCTGTGCTGC 
GCGGCCCCCG CGCATGCATT GCAGTGTCGA GATGGCTATG AACCCTGTGT AAATGAAGGA 
ATGTGTGTTA CCTACCACAA TGGCACAGGA TACTGCAAAT GTCCAGAAGG CTTCTTGGGG 
GAATATTGTC AACATCGAGA CCCCTGTGAG AAGAACCGCT GCCAGAATGG TGGGACTTGT 
GTGGCCCAGG CCATGCTGGG GAAAGCCATG TGCCGATGTG CCTCAGGGTT TACAGGAGAG 
GACTGCCAGT ACTCGACATC TCATCCATGC TTTGTGTCTC GACCCTGCCT GAATGGCGGC 
ACATGCCATA TGCTCAGCCG GGATACCTAT GAGTGCACCT GTCAAGTCGG GTTTACAGGT 
AAGGAGTGCC AATGGACCGA TGCCTGCCTG TCTCATCCCT GTGCAAATGG AAGTACCTGT 
ACCACTGTGG CCAACCAGTT CTCCTGCAAA TGCCTCACAG GCTTCACAGG GCAGAAGTGT 
GAGACTGATG TCAATGAGTG TGACATTCCA GGACACTGCC AGCATGGTGG CACCTGCCTC 
AACCTGCCTG GTTCCTACCA GTGCCAGTGC CCTCAGGGCT TCACAGGCCA GTACTGTGAC 
AGCCTGTATG TGCCCTGTGC ACCCTCGCCT TGTGTCAATG GAGGCACCTG TCGGCAGACT 
GGTGACTTCA CTTTTGAGTG CAACTGCCTT CCAGAAATAA GAGGAACAGA GCTCTGGGAA 
AGAGACAGGC AAGTCTGGAA TGGAAAAGAA CACGATGAGA ATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016780043.1
CDS	878..2122
Translation	MRTGPVTRSRSLRAELGPALLPAGNPWVPKAAGPRPRRQWGGACGGRRRLRRKDTRGCFAAHPRKFQPNFGRRLRRRPRSGGLGGRGVQAFAPGLRSVAPGPEPLKQEEGRREWGSSIGTPSPCGSAQAAAAAAAEEATEKMPALRPALLWALLALWLCCAAPAHALQCRDGYEPCVNEGMCVTYHNGTGYCKCPEGFLGEYCQHRDPCEKNRCQNGGTCVAQAMLGKAMCRCASGFTGEDCQYSTSHPCFVSRPCLNGGTCHMLSRDTYECTCQVGFTGKECQWTDACLSHPCANGSTCTTVANQFSCKCLTGFTGQKCETDVNECDIPGHCQHGGTCLNLPGSYQCQCPQGFTGQYCDSLYVPCAPSPCVNGGTCRQTGDFTFECNCLPEIRGTELWERDRQVWNGKEHDEN

Target ORF information:

RefSeq Version	XM_016924554.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes notch 2 (NOTCH2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016924554.1

ORF Insert Sequence:

ATGCGGACGG GGCCAGTGAC TCGTAGTAGA TCCCTCCGCG CGGAGCTCGG GCCGGCGCTT 
CTTCCTGCGG GAAACCCCTG GGTGCCCAAG GCGGCGGGGC CGAGGCCGCG GCGACAGTGG 
GGCGGGGCTT GCGGTGGGAG GAGGCGGCTG AGGCGGAAGG ACACACGAGG CTGCTTCGCT 
GCACACCCGA GAAAGTTTCA GCCAAACTTC GGGCGGCGGC TGAGGCGGCG GCCGAGGAGC 
GGCGGACTCG GGGGCCGGGG AGTCCAGGCA TTTGCGCCTG GGCTTCGGAG CGTAGCGCCA 
GGGCCTGAGC CTTTGAAGCA GGAGGAGGGG AGGAGAGAGT GGGGCTCCTC TATCGGGACC 
CCCTCCCCAT GTGGATCTGC CCAGGCGGCG GCGGCGGCGG CGGCGGAGGA GGCGACCGAG 
AAGATGCCCG CCCTGCGCCC CGCTCTGCTG TGGGCGCTGC TGGCGCTCTG GCTGTGCTGC 
GCGGCCCCCG CGCATGCATT GCAGTGTCGA GATGGCTATG AACCCTGTGT AAATGAAGGA 
ATGTGTGTTA CCTACCACAA TGGCACAGGA TACTGCAAAT GTCCAGAAGG CTTCTTGGGG 
GAATATTGTC AACATCGAGA CCCCTGTGAG AAGAACCGCT GCCAGAATGG TGGGACTTGT 
GTGGCCCAGG CCATGCTGGG GAAAGCCATG TGCCGATGTG CCTCAGGGTT TACAGGAGAG 
GACTGCCAGT ACTCGACATC TCATCCATGC TTTGTGTCTC GACCCTGCCT GAATGGCGGC 
ACATGCCATA TGCTCAGCCG GGATACCTAT GAGTGCACCT GTCAAGTCGG GTTTACAGGT 
AAGGAGTGCC AATGGACCGA TGCCTGCCTG TCTCATCCCT GTGCAAATGG AAGTACCTGT 
ACCACTGTGG CCAACCAGTT CTCCTGCAAA TGCCTCACAG GCTTCACAGG GCAGAAGTGT 
GAGACTGATG TCAATGAGTG TGACATTCCA GGACACTGCC AGCATGGTGG CACCTGCCTC 
AACCTGCCTG GTTCCTACCA GTGCCAGTGC CCTCAGGGCT TCACAGGCCA GTACTGTGAC 
AGCCTGTATG TGCCCTGTGC ACCCTCGCCT TGTGTCAATG GAGGCACCTG TCGGCAGACT 
GGTGACTTCA CTTTTGAGTG CAACTGCCTT CCAGAAATAA GAGGAACAGA GCTCTGGGAA 
AGAGACAGGC AAGTCTGGAA TGGAAAAGAA CACGATGAGA ATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	NOTCH2
Entrez Gene ID	457173
Full Name	notch 2
Gene Type	protein-coding
Organism	Pan troglodytes(chimpanzee)

	NOTCH2	XP_001114248.1
Rattus norvegicus (Norway rat)	Notch2	NP_077334.1
Homo sapiens (human)	NOTCH2	NP_077719.2
Canis lupus familiaris (dog)	NOTCH2	XP_005630745.1
Bos taurus (cattle)	NOTCH2	XP_002686160.2
Xenopus tropicalis (tropical clawed frog)	LOC100486344	XP_002939126.2
Pan troglodytes (chimpanzee)	NOTCH2	XP_513697.3
Mus musculus (house mouse)	Notch2	NP_035058.2
Gallus gallus (chicken)	NOTCH2	NP_001238962.1
Danio rerio (zebrafish)	notch2	NP_001108566.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

NOTCH2 cDNA ORF clone, Pan troglodytes(chimpanzee)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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