ANKLE2 cDNA ORF clone, Pan troglodytes(chimpanzee)

Gene
Clones
gRNAs

Summary

Gene Symbol	ANKLE2
Entrez Gene ID	452390
Full Name	ankyrin repeat and LEM domain containing 2
Gene Type	protein-coding
Organism	Pan troglodytes(chimpanzee)

mRNA and Protein(s)

mRNA	Protein	Name
XM_024348186.1	XP_024203954.1	ankyrin repeat and LEM domain-containing protein 2 isoform X2
XM_024348184.1	XP_024203952.1	ankyrin repeat and LEM domain-containing protein 2 isoform X1
XM_016924701.1	XP_016780190.1	ankyrin repeat and LEM domain-containing protein 2
XM_024348187.1	XP_024203955.1	ankyrin repeat and LEM domain-containing protein 2 isoform X3
XM_024348185.1	XP_024203953.1	ankyrin repeat and LEM domain-containing protein 2 isoform X2

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Major chimpanzee-specific structural changes in sperm development-associated genes.
Kim RN, Kim DW, Choi SH, Chae SH, Nam SH, Kim DW, Kim A, Kang A, Park KH, Lee YS, Hirai M, Suzuki Y, Sugano S, Hashimoto K, Kim DS, Park HS
Functional & integrative genomics11(3)507-17(2011 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following ANKLE2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ANKLE2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OPc66894	XM_016924701.1 Latest version!	Pan troglodytes ankyrin repeat and LEM domain containing 2 (ANKLE2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OPc96705	XM_024348187.1 Latest version!	Pan troglodytes ankyrin repeat and LEM domain containing 2 (ANKLE2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OPc96703	XM_024348184.1 Latest version!	Pan troglodytes ankyrin repeat and LEM domain containing 2 (ANKLE2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OPc96704	XM_024348185.1 Latest version!	Pan troglodytes ankyrin repeat and LEM domain containing 2 (ANKLE2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OPc96704	XM_024348186.1 Latest version!	Pan troglodytes ankyrin repeat and LEM domain containing 2 (ANKLE2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ANKLE2 ( XM_016924701.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016780190.1 Pan troglodytes ankyrin repeat and LEM domain containing 2 (ANKLE2), mRNA.

Price & Availability↑

CloneID	OPc66894
Clone ID Related Accession (Same CDS sequence)	XM_016924701.1
Accession Version	XM_016924701.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2631bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	ankyrin repeat and LEM domain-containing protein 2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006479.4) and transcript sequence (AK306974.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 132 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGACAATGG ATGCTCTGTT GGCTCGATTG AAACTTCTGA ATCCAGATGA CCTTAGAGAA 
GAAATCGTCA AAGCTGGATT GAAATGTGGA CCCATTACAT CAACTACAAG GTTCATTTTT 
GAGAAAAAAT TGGCTCAGGC TTTACTGGAG CAAGGAGGAA GGCTGTCTTC TTTCTACCAC 
CATGAGGCAG GTGTCACAAC TCTCAGCCAA GACCCACAAA GGATTTTGAA GCCAGCTGAA 
GGGAACCCAA CTGATCAGGC TGGTTTTTCT GAAGACAGAG ATTTTGGTTA CAGTGTGGGC 
CTGAATCCTC CAGAGGAGGA AGCTGTGACA TCCAAGACCT GCTCGGTGCC CCCTAGTGCC 
ACCGACACCT ACAGAGCTGG AGCGACTGCG TCTAAGGAGC CGCCCCTGTA CTATGGGGTG 
TGTCCAGTGT ATGAGGACGT CCCAGCGAGA AATGAAAGGA TCTATGTTTA TGAAAATAAA 
AAGGAAGCAT TGCAAGCTGT CAAGATGATC AAAGGGTCCC GATTTAAAGC TTTTTCTACC 
AGAGAAGACG CTGAGAAATT TGCTAGAGGA ATTTGTGATT ATTTCCCTTC TCCAAGCAAA 
ACGTCCTTAC CACTGTCTCC TGTGAAAACA GCTCCACTCT TTAGCAATGA CGGGTTGAAA 
GATGGTTTGT GCTTGTCGGA ATCAGAAACA GTCAACAAAG AGCGAGCGAA CAGTTACAAA 
AATCCCCGCA CGCAGGACCT CACCGCCAAG CTTCGGAAAG CTGTGGAGAA GGGAGAGGAG 
GACACCTTTT CTGACCTTAT CTGGAGCAAC CCCCGGTATC TGATAGGCTC AGGAGACAAC 
CCCACTATCG TGCAGGAAGG GTGCAGGTAC AACGTGATGC ATGTTGCTGC CAAAGAGAAC 
CAGGCTTCCA TCTGCCAGCT GACTCTGGAC GTCCTGGAGA ACCCTGACTT CATGAGGCTG 
ATGTACCCTG ATGACGACGA GGCCATGCTG CAGAAGCGTA TCCGTTACGT GGTGGACCTG 
TACCTCAACA CCCCCGACAA GATGGGCTAC GACACACCGT TGCATTTTGC TTGTAAGTTT 
GGAAATGCAG ATGTAGTCAA CGTGCTTTCG TCACACCATT TGATTGTAAA AAACTCAAGG 
AATAAATATG ATAAAACACC TGAAGATGTA ATTTGTGAAA GAAGCAAAAA TAAATCTGTG 
GAACTGAAGG AGCGGATCAG AGAGTATTTA AAGGGCCACT ACTACGTGCC CCTCCTGAGA 
GCGGAAGAGA CTTCTTCTCC AGTCATCGGG GAGCTGTGGT CCCCAGACCA GACGGCCGAG 
GCCTCTCACG TCAGCCACTA TGGAGGCAGC CCCAGAGACC CGGTGCTGAC CCTGAGAGCC 
TTCGCAGGGC CCCTGAGTCC AGCCAAGGCA GAAGATTTTC GCAAGCTCTG GAAAACTCCA 
CCTCGAGAGA AAGCAGGCTT CCTTCACCAC GTCAAGAAGT CGGACCCGGA AAGAGGCTTT 
GAGAGAGTGG GAAGGGAGCT AGCTCATGAG CTGGGGTATC CCTGGGTTGA ATACTGGGAA 
TTTCTGGGCT GTTTTGTTGA TCTGTCTTCC CAGGAAGGCC TGCAAAGACT AGAAGAATAT 
CTCACACAGC AGGAAATAGG CAAAAAGGCT CAACAAGAAA CAGGAGAACG GGAAGCCTCC 
TGCCGAGATA AAGCCACCAC GTCTGGCAGC AATTCCATTT CCGTGAGGGC GTTTCTAGAT 
GAAGATGACA TGAGCTTGGA AGAAATAAAA AATCGGCAAA ATGCAGCTCG AAATAACAGC 
CCGCCCACAG TCGGTGCTTT TGGACATACG AGGTGCAGCA CCTTCCCCTT GGAGCAGGAG 
GCAGACCTCA TAGAAGCCGC CGAGCCGGGC GGTCCACACA GCAGCAGAAA TGGGCTCTGC 
CATCGTCTGA GTCACAGCAG GACCCTGGCG GGCAAGAGAC CAAAGGCCCC CCGTGGGGAG 
GAAGCCCGTC TGCCATCTGT CTCTGATTTG ACTGTTGCGT TTGATAAACT GAATTTGCAA 
AATGTAGGAA GTAGCGTTTC CAAGACACCA GATGAAAATA CAAAAACTAA AGATCAGATC 
CTGACTTCAA GAATCAATGC AGTAGAAAGA GACTTGTTAG AGCCTTCTCC CGCAGACCAA 
CTCGGGAATG GCCACAGGAG GACAGAAAGT GAAATGTCAG CCAGGATCGC TAAAATGTCC 
TTGAGTCCCA GCAGCCCCAG GCACGAGGAT CAGCTCAAGG TCACGAGGGA ACCGGCCAGG 
CGGCTCTTCC TTTTTGGAGA GGAGCCGTCA AAACTCGATC AGGATGTTTT GGCCGCTCTT 
GAATGTGCAG ACGTTGACCC CCATCAGTTC CCGGCCGTGC ACAGATGGAA GAGTGCTGTC 
CTGTGCTACT CACCCTCGGA CAGACAGAGT TGGCCCAGTC CCGCGGTGAA AGGAAAGTTC 
AAGTCTCAGC TGCCAGATCT CAGTGGCCCT CACAGCTACA GTCCAGGGAG AAACAGCGTG 
GCTGGAAGCA ACCCCGCAAA GCCGGGCCTG GGCAGTCCTG GGCGCTACAG CCCCGTGCAC 
AGGAGCCAGC TCCGCAGGAT GGCGCGCCTG GCTGAGCTTG CCGCCCTGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016780190.1
CDS	125..2755
Translation	MTMDALLARLKLLNPDDLREEIVKAGLKCGPITSTTRFIFEKKLAQALLEQGGRLSSFYHHEAGVTTLSQDPQRILKPAEGNPTDQAGFSEDRDFGYSVGLNPPEEEAVTSKTCSVPPSATDTYRAGATASKEPPLYYGVCPVYEDVPARNERIYVYENKKEALQAVKMIKGSRFKAFSTREDAEKFARGICDYFPSPSKTSLPLSPVKTAPLFSNDGLKDGLCLSESETVNKERANSYKNPRTQDLTAKLRKAVEKGEEDTFSDLIWSNPRYLIGSGDNPTIVQEGCRYNVMHVAAKENQASICQLTLDVLENPDFMRLMYPDDDEAMLQKRIRYVVDLYLNTPDKMGYDTPLHFACKFGNADVVNVLSSHHLIVKNSRNKYDKTPEDVICERSKNKSVELKERIREYLKGHYYVPLLRAEETSSPVIGELWSPDQTAEASHVSHYGGSPRDPVLTLRAFAGPLSPAKAEDFRKLWKTPPREKAGFLHHVKKSDPERGFERVGRELAHELGYPWVEYWEFLGCFVDLSSQEGLQRLEEYLTQQEIGKKAQQETGEREASCRDKATTSGSNSISVRAFLDEDDMSLEEIKNRQNAARNNSPPTVGAFGHTRCSTFPLEQEADLIEAAEPGGPHSSRNGLCHRLSHSRTLAGKRPKAPRGEEARLPSVSDLTVAFDKLNLQNVGSSVSKTPDENTKTKDQILTSRINAVERDLLEPSPADQLGNGHRRTESEMSARIAKMSLSPSSPRHEDQLKVTREPARRLFLFGEEPSKLDQDVLAALECADVDPHQFPAVHRWKSAVLCYSPSDRQSWPSPAVKGKFKSQLPDLSGPHSYSPGRNSVAGSNPAKPGLGSPGRYSPVHRSQLRRMARLAELAAL

Target ORF information:

RefSeq Version	XM_016924701.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes ankyrin repeat and LEM domain containing 2 (ANKLE2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016924701.1

ORF Insert Sequence:

ATGACAATGG ATGCTCTGTT GGCTCGATTG AAACTTCTGA ATCCAGATGA CCTTAGAGAA 
GAAATCGTCA AAGCTGGATT GAAATGTGGA CCCATTACAT CAACTACAAG GTTCATTTTT 
GAGAAAAAAT TGGCTCAGGC TTTACTGGAG CAAGGAGGAA GGCTGTCTTC TTTCTACCAC 
CATGAGGCAG GTGTCACAAC TCTCAGCCAA GACCCACAAA GGATTTTGAA GCCAGCTGAA 
GGGAACCCAA CTGATCAGGC TGGTTTTTCT GAAGACAGAG ATTTTGGTTA CAGTGTGGGC 
CTGAATCCTC CAGAGGAGGA AGCTGTGACA TCCAAGACCT GCTCGGTGCC CCCTAGTGCC 
ACCGACACCT ACAGAGCTGG AGCGACTGCG TCTAAGGAGC CGCCCCTGTA CTATGGGGTG 
TGTCCAGTGT ATGAGGACGT CCCAGCGAGA AATGAAAGGA TCTATGTTTA TGAAAATAAA 
AAGGAAGCAT TGCAAGCTGT CAAGATGATC AAAGGGTCCC GATTTAAAGC TTTTTCTACC 
AGAGAAGACG CTGAGAAATT TGCTAGAGGA ATTTGTGATT ATTTCCCTTC TCCAAGCAAA 
ACGTCCTTAC CACTGTCTCC TGTGAAAACA GCTCCACTCT TTAGCAATGA CGGGTTGAAA 
GATGGTTTGT GCTTGTCGGA ATCAGAAACA GTCAACAAAG AGCGAGCGAA CAGTTACAAA 
AATCCCCGCA CGCAGGACCT CACCGCCAAG CTTCGGAAAG CTGTGGAGAA GGGAGAGGAG 
GACACCTTTT CTGACCTTAT CTGGAGCAAC CCCCGGTATC TGATAGGCTC AGGAGACAAC 
CCCACTATCG TGCAGGAAGG GTGCAGGTAC AACGTGATGC ATGTTGCTGC CAAAGAGAAC 
CAGGCTTCCA TCTGCCAGCT GACTCTGGAC GTCCTGGAGA ACCCTGACTT CATGAGGCTG 
ATGTACCCTG ATGACGACGA GGCCATGCTG CAGAAGCGTA TCCGTTACGT GGTGGACCTG 
TACCTCAACA CCCCCGACAA GATGGGCTAC GACACACCGT TGCATTTTGC TTGTAAGTTT 
GGAAATGCAG ATGTAGTCAA CGTGCTTTCG TCACACCATT TGATTGTAAA AAACTCAAGG 
AATAAATATG ATAAAACACC TGAAGATGTA ATTTGTGAAA GAAGCAAAAA TAAATCTGTG 
GAACTGAAGG AGCGGATCAG AGAGTATTTA AAGGGCCACT ACTACGTGCC CCTCCTGAGA 
GCGGAAGAGA CTTCTTCTCC AGTCATCGGG GAGCTGTGGT CCCCAGACCA GACGGCCGAG 
GCCTCTCACG TCAGCCACTA TGGAGGCAGC CCCAGAGACC CGGTGCTGAC CCTGAGAGCC 
TTCGCAGGGC CCCTGAGTCC AGCCAAGGCA GAAGATTTTC GCAAGCTCTG GAAAACTCCA 
CCTCGAGAGA AAGCAGGCTT CCTTCACCAC GTCAAGAAGT CGGACCCGGA AAGAGGCTTT 
GAGAGAGTGG GAAGGGAGCT AGCTCATGAG CTGGGGTATC CCTGGGTTGA ATACTGGGAA 
TTTCTGGGCT GTTTTGTTGA TCTGTCTTCC CAGGAAGGCC TGCAAAGACT AGAAGAATAT 
CTCACACAGC AGGAAATAGG CAAAAAGGCT CAACAAGAAA CAGGAGAACG GGAAGCCTCC 
TGCCGAGATA AAGCCACCAC GTCTGGCAGC AATTCCATTT CCGTGAGGGC GTTTCTAGAT 
GAAGATGACA TGAGCTTGGA AGAAATAAAA AATCGGCAAA ATGCAGCTCG AAATAACAGC 
CCGCCCACAG TCGGTGCTTT TGGACATACG AGGTGCAGCA CCTTCCCCTT GGAGCAGGAG 
GCAGACCTCA TAGAAGCCGC CGAGCCGGGC GGTCCACACA GCAGCAGAAA TGGGCTCTGC 
CATCGTCTGA GTCACAGCAG GACCCTGGCG GGCAAGAGAC CAAAGGCCCC CCGTGGGGAG 
GAAGCCCGTC TGCCATCTGT CTCTGATTTG ACTGTTGCGT TTGATAAACT GAATTTGCAA 
AATGTAGGAA GTAGCGTTTC CAAGACACCA GATGAAAATA CAAAAACTAA AGATCAGATC 
CTGACTTCAA GAATCAATGC AGTAGAAAGA GACTTGTTAG AGCCTTCTCC CGCAGACCAA 
CTCGGGAATG GCCACAGGAG GACAGAAAGT GAAATGTCAG CCAGGATCGC TAAAATGTCC 
TTGAGTCCCA GCAGCCCCAG GCACGAGGAT CAGCTCAAGG TCACGAGGGA ACCGGCCAGG 
CGGCTCTTCC TTTTTGGAGA GGAGCCGTCA AAACTCGATC AGGATGTTTT GGCCGCTCTT 
GAATGTGCAG ACGTTGACCC CCATCAGTTC CCGGCCGTGC ACAGATGGAA GAGTGCTGTC 
CTGTGCTACT CACCCTCGGA CAGACAGAGT TGGCCCAGTC CCGCGGTGAA AGGAAAGTTC 
AAGTCTCAGC TGCCAGATCT CAGTGGCCCT CACAGCTACA GTCCAGGGAG AAACAGCGTG 
GCTGGAAGCA ACCCCGCAAA GCCGGGCCTG GGCAGTCCTG GGCGCTACAG CCCCGTGCAC 
AGGAGCCAGC TCCGCAGGAT GGCGCGCCTG GCTGAGCTTG CCGCCCTGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANKLE2 ( XM_024348187.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_024203955.1 Pan troglodytes ankyrin repeat and LEM domain containing 2 (ANKLE2), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OPc96705
Clone ID Related Accession (Same CDS sequence)	XM_024348187.1
Accession Version	XM_024348187.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2817bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	ankyrin repeat and LEM domain-containing protein 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036891.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTGGC CGCGGCTGGC GGCGGCCGAG TGGGCGGCGC TGGCCTGGGA GCTGCTGGGC 
GCCTCGGTGC TGCTGATCGC TGTGCGGTGG CTGGTGCGGC GGCTGGGCCC GCGGCCGGGA 
GGTCTGGGCC GCAGCGGGAC CCCAGTTCCT CCGCCAAGCG CGGCCGCCGC CCCCGCCCCA 
GGTGAAATGA CAATGGATGC TCTGTTGGCT CGATTGAAAC TTCTGAATCC AGATGACCTT 
AGAGAAGAAA TCGTCAAAGC TGGATTGAAA TGTGGACCCA TTACATCAAC TACAAGGTTC 
ATTTTTGAGA AAAAATTGGC TCAGGCTTTA CTGGAGCAAG GAGGAAGGCT GTCTTCTTTC 
TACCACCATG AGGCAGGTGT CACAACTCTC AGCCAAGACC CACAAAGGAT TTTGAAGCCA 
GCTGAAGGGA ACCCAACTGA TCAGGCTGGT TTTTCTGAAG ACAGAGATTT TGGTTACAGT 
GTGGGCCTGA ATCCTCCAGA GGAGGAAGCT GTGACATCCA AGACCTGCTC GGTGCCCCCT 
AGTGCCACCG ACACCTACAG AGCTGGAGCG ACTGCGTCTA AGGAGCCGCC CCTGTACTAT 
GGGGTGTGTC CAGTGTATGA GGACGTCCCA GCGAGAAATG AAAGGATCTA TGTTTATGAA 
AATAAAAAGG AAGCATTGCA AGCTGTCAAG ATGATCAAAG GGTCCCGATT TAAAGCTTTT 
TCTACCAGAG AAGACGCTGA GAAATTTGCT AGAGGAATTT GTGATTATTT CCCTTCTCCA 
AGCAAAACGT CCTTACCACT GTCTCCTGTG AAAACAGCTC CACTCTTTAG CAATGACGGG 
TTGAAAGATG GTTTGTGCTT GTCGGAATCA GAAACAGTCA ACAAAGAGCG AGCGAACAGT 
TACAAAAATC CCCGCACGCA GGACCTCACC GCCAAGCTTC GGAAAGCTGT GGAGAAGGGA 
GAGGAGGACA CCTTTTCTGA CCTTATCTGG AGCAACCCCC GGTATCTGAT AGGCTCAGGA 
GACAACCCCA CTATCGTGCA GGAAGGGTGC AGGTACAACG TGATGCATGT TGCTGCCAAA 
GAGAACCAGG CTTCCATCTG CCAGCTGACT CTGGACGTCC TGGAGAACCC TGACTTCATG 
AGGCTGATGT ACCCTGATGA CGACGAGGCC ATGCTGCAGA AGCGTATCCG TTACGTGGTG 
GACCTGTACC TCAACACCCC CGACAAGATG GGCTACGACA CACCGTTGCA TTTTGCTTGT 
AAGTTTGGAA ATGCAGATGT AGTCAACGTG CTTTCGTCAC ACCATTTGAT TGTAAAAAAC 
TCAAGGAATA AATATGATAA AACACCTGAA GATGTAATTT GTGAAAGAAG CAAAAATAAA 
TCTGTGGAAC TGAAGGAGCG GATCAGAGAG TATTTAAAGG GCCACTACTA CGTGCCCCTC 
CTGAGAGCGG AAGAGACTTC TTCTCCAGTC ATCGGGGAGC TGTGGTCCCC AGACCAGACG 
GCCGAGGCCT CTCACGTCAG CCACTATGGA GGCAGCCCCA GAGACCCGGT GCTGACCCTG 
AGAGCCTTCG CAGGGCCCCT GAGTCCAGCC AAGGCAGAAG ATTTTCGCAA GCTCTGGAAA 
ACTCCACCTC GAGAGAAAGC AGGCTTCCTT CACCACGTCA AGAAGTCGGA CCCGGAAAGA 
GGCTTTGAGA GAGTGGGAAG GGAGCTAGCT CATGAGCTGG GGTATCCCTG GGTTGAATAC 
TGGGAATTTC TGGGCTGTTT TGTTGATCTG TCTTCCCAGG AAGGCCTGCA AAGACTAGAA 
GAATATCTCA CACAGCAGGA AATAGGCAAA AAGGCTCAAC AAGAAACAGG AGAACGGGAA 
GCCTCCTGCC GAGATAAAGC CACCACGTCT GGCAGCAATT CCATTTCCGT GAGGGCGTTT 
CTAGATGAAG ATGACATGAG CTTGGAAGAA ATAAAAAATC GGCAAAATGC AGCTCGAAAT 
AACAGCCCGC CCACAGTCGG TGCTTTTGGA CATACGAGGT GCAGCACCTT CCCCTTGGAG 
CAGGAGGCAG ACCTCATAGA AGCCGCCGAG CCGGGCGGTC CACACAGCAG CAGAAATGGG 
CTCTGCCATC GTCTGAGTCA CAGCAGGACC CTGGCGGGCA AGAGACCAAA GGCCCCCCGT 
GGGGAGGAAG CCCGTCTGCC ATCTGTCTCT GATTTGACTG TTGCGTTTGA TAAACTGAAT 
TTGCAAAATG TAGGAAGTAG CGTTTCCAAG ACACCAGATG AAAATACAAA AACTAAAGAT 
CAGATCCTGA CTTCAAGAAT CAATGCAGTA GAAAGAGACT TGTTAGAGCC TTCTCCCGCA 
GACCAACTCG GGAATGGCCA CAGGAGGACA GAAAGTGAAA TGTCAGCCAG GATCGCTAAA 
ATGTCCTTGA GTCCCAGCAG CCCCAGGCAC GAGGATCAGC TCAAGGTCAC GAGGGAACCG 
GCCAGGCGGC TCTTCCTTTT TGGAGAGGAG CCGTCAAAAC TCGATCAGGA TGTTTTGGCC 
GCTCTTGAAT GTGCAGACGT TGACCCCCAT CAGTTCCCGG CCGTGCACAG ATGGAAGAGT 
GCTGTCCTGT GCTACTCACC CTCGGACAGA CAGAGTTGGC CCAGTCCCGC GGTGAAAGGA 
AAGTTCAAGT CTCAGCTGCC AGATCTCAGT GGCCCTCACA GCTACAGTCC AGGGAGAAAC 
AGCGTGGCTG GAAGCAACCC CGCAAAGCCG GGCCTGGGCA GTCCTGGGCG CTACAGCCCC 
GTGCACAGGA GCCAGCTCCG CAGGATGGCG CGCCTGGCTG AGCTTGCCGC CCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024203955.1
CDS	85..2901
Translation	MLWPRLAAAEWAALAWELLGASVLLIAVRWLVRRLGPRPGGLGRSGTPVPPPSAAAAPAPGEMTMDALLARLKLLNPDDLREEIVKAGLKCGPITSTTRFIFEKKLAQALLEQGGRLSSFYHHEAGVTTLSQDPQRILKPAEGNPTDQAGFSEDRDFGYSVGLNPPEEEAVTSKTCSVPPSATDTYRAGATASKEPPLYYGVCPVYEDVPARNERIYVYENKKEALQAVKMIKGSRFKAFSTREDAEKFARGICDYFPSPSKTSLPLSPVKTAPLFSNDGLKDGLCLSESETVNKERANSYKNPRTQDLTAKLRKAVEKGEEDTFSDLIWSNPRYLIGSGDNPTIVQEGCRYNVMHVAAKENQASICQLTLDVLENPDFMRLMYPDDDEAMLQKRIRYVVDLYLNTPDKMGYDTPLHFACKFGNADVVNVLSSHHLIVKNSRNKYDKTPEDVICERSKNKSVELKERIREYLKGHYYVPLLRAEETSSPVIGELWSPDQTAEASHVSHYGGSPRDPVLTLRAFAGPLSPAKAEDFRKLWKTPPREKAGFLHHVKKSDPERGFERVGRELAHELGYPWVEYWEFLGCFVDLSSQEGLQRLEEYLTQQEIGKKAQQETGEREASCRDKATTSGSNSISVRAFLDEDDMSLEEIKNRQNAARNNSPPTVGAFGHTRCSTFPLEQEADLIEAAEPGGPHSSRNGLCHRLSHSRTLAGKRPKAPRGEEARLPSVSDLTVAFDKLNLQNVGSSVSKTPDENTKTKDQILTSRINAVERDLLEPSPADQLGNGHRRTESEMSARIAKMSLSPSSPRHEDQLKVTREPARRLFLFGEEPSKLDQDVLAALECADVDPHQFPAVHRWKSAVLCYSPSDRQSWPSPAVKGKFKSQLPDLSGPHSYSPGRNSVAGSNPAKPGLGSPGRYSPVHRSQLRRMARLAELAAL

Target ORF information:

RefSeq Version	XM_024348187.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes ankyrin repeat and LEM domain containing 2 (ANKLE2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024348187.1

ORF Insert Sequence:

ATGCTGTGGC CGCGGCTGGC GGCGGCCGAG TGGGCGGCGC TGGCCTGGGA GCTGCTGGGC 
GCCTCGGTGC TGCTGATCGC TGTGCGGTGG CTGGTGCGGC GGCTGGGCCC GCGGCCGGGA 
GGTCTGGGCC GCAGCGGGAC CCCAGTTCCT CCGCCAAGCG CGGCCGCCGC CCCCGCCCCA 
GGTGAAATGA CAATGGATGC TCTGTTGGCT CGATTGAAAC TTCTGAATCC AGATGACCTT 
AGAGAAGAAA TCGTCAAAGC TGGATTGAAA TGTGGACCCA TTACATCAAC TACAAGGTTC 
ATTTTTGAGA AAAAATTGGC TCAGGCTTTA CTGGAGCAAG GAGGAAGGCT GTCTTCTTTC 
TACCACCATG AGGCAGGTGT CACAACTCTC AGCCAAGACC CACAAAGGAT TTTGAAGCCA 
GCTGAAGGGA ACCCAACTGA TCAGGCTGGT TTTTCTGAAG ACAGAGATTT TGGTTACAGT 
GTGGGCCTGA ATCCTCCAGA GGAGGAAGCT GTGACATCCA AGACCTGCTC GGTGCCCCCT 
AGTGCCACCG ACACCTACAG AGCTGGAGCG ACTGCGTCTA AGGAGCCGCC CCTGTACTAT 
GGGGTGTGTC CAGTGTATGA GGACGTCCCA GCGAGAAATG AAAGGATCTA TGTTTATGAA 
AATAAAAAGG AAGCATTGCA AGCTGTCAAG ATGATCAAAG GGTCCCGATT TAAAGCTTTT 
TCTACCAGAG AAGACGCTGA GAAATTTGCT AGAGGAATTT GTGATTATTT CCCTTCTCCA 
AGCAAAACGT CCTTACCACT GTCTCCTGTG AAAACAGCTC CACTCTTTAG CAATGACGGG 
TTGAAAGATG GTTTGTGCTT GTCGGAATCA GAAACAGTCA ACAAAGAGCG AGCGAACAGT 
TACAAAAATC CCCGCACGCA GGACCTCACC GCCAAGCTTC GGAAAGCTGT GGAGAAGGGA 
GAGGAGGACA CCTTTTCTGA CCTTATCTGG AGCAACCCCC GGTATCTGAT AGGCTCAGGA 
GACAACCCCA CTATCGTGCA GGAAGGGTGC AGGTACAACG TGATGCATGT TGCTGCCAAA 
GAGAACCAGG CTTCCATCTG CCAGCTGACT CTGGACGTCC TGGAGAACCC TGACTTCATG 
AGGCTGATGT ACCCTGATGA CGACGAGGCC ATGCTGCAGA AGCGTATCCG TTACGTGGTG 
GACCTGTACC TCAACACCCC CGACAAGATG GGCTACGACA CACCGTTGCA TTTTGCTTGT 
AAGTTTGGAA ATGCAGATGT AGTCAACGTG CTTTCGTCAC ACCATTTGAT TGTAAAAAAC 
TCAAGGAATA AATATGATAA AACACCTGAA GATGTAATTT GTGAAAGAAG CAAAAATAAA 
TCTGTGGAAC TGAAGGAGCG GATCAGAGAG TATTTAAAGG GCCACTACTA CGTGCCCCTC 
CTGAGAGCGG AAGAGACTTC TTCTCCAGTC ATCGGGGAGC TGTGGTCCCC AGACCAGACG 
GCCGAGGCCT CTCACGTCAG CCACTATGGA GGCAGCCCCA GAGACCCGGT GCTGACCCTG 
AGAGCCTTCG CAGGGCCCCT GAGTCCAGCC AAGGCAGAAG ATTTTCGCAA GCTCTGGAAA 
ACTCCACCTC GAGAGAAAGC AGGCTTCCTT CACCACGTCA AGAAGTCGGA CCCGGAAAGA 
GGCTTTGAGA GAGTGGGAAG GGAGCTAGCT CATGAGCTGG GGTATCCCTG GGTTGAATAC 
TGGGAATTTC TGGGCTGTTT TGTTGATCTG TCTTCCCAGG AAGGCCTGCA AAGACTAGAA 
GAATATCTCA CACAGCAGGA AATAGGCAAA AAGGCTCAAC AAGAAACAGG AGAACGGGAA 
GCCTCCTGCC GAGATAAAGC CACCACGTCT GGCAGCAATT CCATTTCCGT GAGGGCGTTT 
CTAGATGAAG ATGACATGAG CTTGGAAGAA ATAAAAAATC GGCAAAATGC AGCTCGAAAT 
AACAGCCCGC CCACAGTCGG TGCTTTTGGA CATACGAGGT GCAGCACCTT CCCCTTGGAG 
CAGGAGGCAG ACCTCATAGA AGCCGCCGAG CCGGGCGGTC CACACAGCAG CAGAAATGGG 
CTCTGCCATC GTCTGAGTCA CAGCAGGACC CTGGCGGGCA AGAGACCAAA GGCCCCCCGT 
GGGGAGGAAG CCCGTCTGCC ATCTGTCTCT GATTTGACTG TTGCGTTTGA TAAACTGAAT 
TTGCAAAATG TAGGAAGTAG CGTTTCCAAG ACACCAGATG AAAATACAAA AACTAAAGAT 
CAGATCCTGA CTTCAAGAAT CAATGCAGTA GAAAGAGACT TGTTAGAGCC TTCTCCCGCA 
GACCAACTCG GGAATGGCCA CAGGAGGACA GAAAGTGAAA TGTCAGCCAG GATCGCTAAA 
ATGTCCTTGA GTCCCAGCAG CCCCAGGCAC GAGGATCAGC TCAAGGTCAC GAGGGAACCG 
GCCAGGCGGC TCTTCCTTTT TGGAGAGGAG CCGTCAAAAC TCGATCAGGA TGTTTTGGCC 
GCTCTTGAAT GTGCAGACGT TGACCCCCAT CAGTTCCCGG CCGTGCACAG ATGGAAGAGT 
GCTGTCCTGT GCTACTCACC CTCGGACAGA CAGAGTTGGC CCAGTCCCGC GGTGAAAGGA 
AAGTTCAAGT CTCAGCTGCC AGATCTCAGT GGCCCTCACA GCTACAGTCC AGGGAGAAAC 
AGCGTGGCTG GAAGCAACCC CGCAAAGCCG GGCCTGGGCA GTCCTGGGCG CTACAGCCCC 
GTGCACAGGA GCCAGCTCCG CAGGATGGCG CGCCTGGCTG AGCTTGCCGC CCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANKLE2 ( XM_024348184.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_024203952.1 Pan troglodytes ankyrin repeat and LEM domain containing 2 (ANKLE2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OPc96703
Clone ID Related Accession (Same CDS sequence)	XM_024348184.1
Accession Version	XM_024348184.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3222bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	ankyrin repeat and LEM domain-containing protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036891.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTGGC CGCGGCTGGC GGCGGCCGAG TGGGCGGCGC TGGCCTGGGA GCTGCTGGGC 
GCCTCGGTGC TGCTGATCGC TGTGCGGTGG CTGGTGCGGC GGCTGGGCCC GCGGCCGGGA 
GGTCTGGGCC GCAGCGGGAC CCCAGTTCCT CCGCCAAGCG CGGCCGCCGC CCCCGCCCCA 
GGTGAAATGA CAATGGATGC TCTGTTGGCT CGATTGAAAC TTCTGAATCC AGATGACCTT 
AGAGAAGAAA TCGTCAAAGC TGGATTGAAA TGTGGACCCA TTACATCAAC TACAAGGTTC 
ATTTTTGAGA AAAAATTGGC TCAGGCTTTA CTGGAGCAAG GAGGAAGGCT GTCTTCTTTC 
TACCACCATG AGGCAGGTGT CACAACTCTC AGCCAAGACC CACAAAGGAT TTTGAAGCCA 
GCTGAAGGGA ACCCAACTGA TCAGGCTGGT TTTTCTGAAG ACAGAGATTT TGGTTACAGT 
GTGGGCCTGA ATCCTCCAGA GGAGGAAGCT GTGACATCCA AGACCTGCTC GGTGCCCCCT 
AGTGCCACCG ACACCTACAG AGCTGGAGCG ACTGCGTCTA AGGAGCCGCC CCTGTACTAT 
GGGGTGTGTC CAGTGTATGA GGACGTCCCA GCGAGAAATG AAAGGATCTA TGTTTATGAA 
AATAAAAAGG AAGCATTGCA AGCTGTCAAG ATGATCAAAG GGTCCCGATT TAAAGCTTTT 
TCTACCAGAG AAGACGCTGA GAAATTTGCT AGAGGAATTT GTGATTATTT CCCTTCTCCA 
AGCAAAACGT CCTTACCACT GTCTCCTGTG AAAACAGCTC CACTCTTTAG CAATGACGGG 
TTGAAAGATG GTTTGTGCTT GTCGGAATCA GAAACAGTCA ACAAAGAGCG AGCGAACAGT 
TACAAAAATC CCCGCACGCA GGACCTCACC GCCAAGCTTC GGAAAGCTGT GGAGAAGGGA 
GAGGAGGACA CCTTTTCTGA CCTTATCTGG AGCAACCCCC GGTATCTGAT AGGCTCAGGA 
GACAACCCCA CTATCGTGCA GGAAGGGTGC AGGTACAACG TGATGCATGT TGCTGCCAAA 
GAGAACCAGG CTTCCATCTG CCAGCTGACT CTGGACGTCC TGGAGAACCC TGACTTCATG 
AGGCTGATGT ACCCTGATGA CGACGAGGCC ATGCTGCAGA AGCGTATCCG TTACGTGGTG 
GACCTGTACC TCAACACCCC CGACAAGATG GGCTACGACA CACCGTTGCA TTTTGCTTGT 
AAGTTTGGAA ATGCAGATGT AGTCAACGTG CTTTCGTCAC ACCATTTGAT TGTAAAAAAC 
TCAAGGAATA AATATGATAA AACACCTGAA GATGTAATTT GTGAAAGAAG CAAAAATAAA 
TCTGTGGAAC TGAAGGAGCG GATCAGAGAG TATTTAAAGG GCCACTACTA CGTGCCCCTC 
CTGAGAGCGG AAGAGACTTC TTCTCCAGTC ATCGGGGAGC TGTGGTCCCC AGACCAGACG 
GCCGAGGCCT CTCACGTCAG CCACTATGGA GGCAGCCCCA GAGACCCGGT GCTGACCCTG 
AGAGCCTTCG CAGGGCCCCT GAGTCCAGCC AAGGCAGAAG ATTTTCGCAA GCTCTGGAAA 
ACTCCACCTC GAGAGAAAGC AGGCTTCCTT CACCACGTCA AGAAGTCGGA CCCGGAAAGA 
GGCTTTGAGA GAGTGGGAAG GGAGCTAGCT CATGAGCTGG GGTATCCCTG GGTTGAATAC 
TGGGAATTTC TGGGCTGTTT TGTTGATCTG TCTTCCCAGG AAGGCCTGCA AAGACTAGAA 
GAATATCTCA CACAGCAGGA AATAGGCAAA AAGGCTCAAC AAGAAACAGG AGAACGGGAA 
GCCTCCTGCC GAGATAAAGC CACCACGTCT GGAAAAAGAC GAGTGCACAT CAGAATACTG 
GTGTATATCA GACACCAGGG CACAAAGCGC CTCGCACGGT GCGTATCAGA CACCAGGACG 
CAGAGCGCTT CACACAGTGC ATATCAGACA CCAGGACGCA GAGAGCTTCA CACGGTGCAT 
TTCAGACACC AGGACGCAGA GCGCTTCACA CGGTGCATAT CAGACACCAG GACGGAGAGC 
GCTTCACACG GTGCATATCA GACACCAGGA CGCAGAGCGC TTCAGACAGT GCATATCAGA 
CACCAGGACG CAGAGCGCTT CACACGGTGC ATATCAGACA CCAGGACGCA GAGAGCTTCG 
CACGGTGCAT ATCAGACACT AGGACGCAGA GAGCTTCGCA CGGTGCGTAT CAGACACCAG 
GATGGAGAGC GCTTCACACG GTGCATATCA GACACCAGGA CGCAGAGCGC TTCACACGGT 
GCATATCAGA CACCAGGACG CAGAGCGCTT CAGACGGTGC ATATCAGACA CCAGGACGCA 
GAGAGCTTCG CACGGTGCAT ATCAGACACT AGGACGCAGA GCGCTTCGCA CGGTGCATAT 
CAGACACCAG GACGCAGAGC GCTTCGCACG GTGCGTATCA GACACCGGGA CGCAGAGAGC 
TTCGCACGGT GCGTATCAGA CACCGGGACG CAGAGAGCTT CGCACGGTGC GTATCAGACA 
CCGGGACGCA GAGAGCTTCA CACGGTGCGT ATCAGACACC GGGACGGAGA GCGCTTCACA 
CGGTGCGTAT CAGACACCGG GACGCAGAGC GCTTCAGACA GTGCATATCA GACACCAGGA 
CGCAGAGCGC TTCACACGGT GCATATCAGA CACCAGGACG CAGAGAGCTT CGCACGGTGC 
ATATCAGACA CTAGGACGCA GAGAGCTTCG CACGGTGCGT ATCAGACACC AGGATGGAGA 
GCGCTTCACA CGGTGCATAT CAGACACCAG GACGCAGAGC GCTTCACACG GTGCATATCA 
GACACCAGGA CGCAGAGCGC TTCAGACGGT GCATATCAGA CACCAGGACG CAGAGAGCTT 
CGCACGGTGC ATATCAGACA CCAGGACGCA GAGCGCTTCG CACGGTGCGT ATCAGACACC 
GGGACGCAGA GAGCTTCACA CGGTGCGTAT CAGACACCGG GACGGAGAGA GCTTCGCACG 
GTGCGTATCA GACACCGGGA CGCAGAGCGC TTCACACGGT GCATATCAGA CACCAGGACG 
CAAACACAAA GCACTTCACA CGGTGTATTT CAAACACCAG GACGCAAAGT GCTTCCAAAG 
CACAGGTGCA CTGAATGGAA AGATCATGAT GGAAGAGGTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024203952.1
CDS	85..3306
Translation	MLWPRLAAAEWAALAWELLGASVLLIAVRWLVRRLGPRPGGLGRSGTPVPPPSAAAAPAPGEMTMDALLARLKLLNPDDLREEIVKAGLKCGPITSTTRFIFEKKLAQALLEQGGRLSSFYHHEAGVTTLSQDPQRILKPAEGNPTDQAGFSEDRDFGYSVGLNPPEEEAVTSKTCSVPPSATDTYRAGATASKEPPLYYGVCPVYEDVPARNERIYVYENKKEALQAVKMIKGSRFKAFSTREDAEKFARGICDYFPSPSKTSLPLSPVKTAPLFSNDGLKDGLCLSESETVNKERANSYKNPRTQDLTAKLRKAVEKGEEDTFSDLIWSNPRYLIGSGDNPTIVQEGCRYNVMHVAAKENQASICQLTLDVLENPDFMRLMYPDDDEAMLQKRIRYVVDLYLNTPDKMGYDTPLHFACKFGNADVVNVLSSHHLIVKNSRNKYDKTPEDVICERSKNKSVELKERIREYLKGHYYVPLLRAEETSSPVIGELWSPDQTAEASHVSHYGGSPRDPVLTLRAFAGPLSPAKAEDFRKLWKTPPREKAGFLHHVKKSDPERGFERVGRELAHELGYPWVEYWEFLGCFVDLSSQEGLQRLEEYLTQQEIGKKAQQETGEREASCRDKATTSGKRRVHIRILVYIRHQGTKRLARCVSDTRTQSASHSAYQTPGRRELHTVHFRHQDAERFTRCISDTRTESASHGAYQTPGRRALQTVHIRHQDAERFTRCISDTRTQRASHGAYQTLGRRELRTVRIRHQDGERFTRCISDTRTQSASHGAYQTPGRRALQTVHIRHQDAESFARCISDTRTQSASHGAYQTPGRRALRTVRIRHRDAESFARCVSDTGTQRASHGAYQTPGRRELHTVRIRHRDGERFTRCVSDTGTQSASDSAYQTPGRRALHTVHIRHQDAESFARCISDTRTQRASHGAYQTPGWRALHTVHIRHQDAERFTRCISDTRTQSASDGAYQTPGRRELRTVHIRHQDAERFARCVSDTGTQRASHGAYQTPGRRELRTVRIRHRDAERFTRCISDTRTQTQSTSHGVFQTPGRKVLPKHRCTEWKDHDGRG

Target ORF information:

RefSeq Version	XM_024348184.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes ankyrin repeat and LEM domain containing 2 (ANKLE2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024348184.1

ORF Insert Sequence:

ATGCTGTGGC CGCGGCTGGC GGCGGCCGAG TGGGCGGCGC TGGCCTGGGA GCTGCTGGGC 
GCCTCGGTGC TGCTGATCGC TGTGCGGTGG CTGGTGCGGC GGCTGGGCCC GCGGCCGGGA 
GGTCTGGGCC GCAGCGGGAC CCCAGTTCCT CCGCCAAGCG CGGCCGCCGC CCCCGCCCCA 
GGTGAAATGA CAATGGATGC TCTGTTGGCT CGATTGAAAC TTCTGAATCC AGATGACCTT 
AGAGAAGAAA TCGTCAAAGC TGGATTGAAA TGTGGACCCA TTACATCAAC TACAAGGTTC 
ATTTTTGAGA AAAAATTGGC TCAGGCTTTA CTGGAGCAAG GAGGAAGGCT GTCTTCTTTC 
TACCACCATG AGGCAGGTGT CACAACTCTC AGCCAAGACC CACAAAGGAT TTTGAAGCCA 
GCTGAAGGGA ACCCAACTGA TCAGGCTGGT TTTTCTGAAG ACAGAGATTT TGGTTACAGT 
GTGGGCCTGA ATCCTCCAGA GGAGGAAGCT GTGACATCCA AGACCTGCTC GGTGCCCCCT 
AGTGCCACCG ACACCTACAG AGCTGGAGCG ACTGCGTCTA AGGAGCCGCC CCTGTACTAT 
GGGGTGTGTC CAGTGTATGA GGACGTCCCA GCGAGAAATG AAAGGATCTA TGTTTATGAA 
AATAAAAAGG AAGCATTGCA AGCTGTCAAG ATGATCAAAG GGTCCCGATT TAAAGCTTTT 
TCTACCAGAG AAGACGCTGA GAAATTTGCT AGAGGAATTT GTGATTATTT CCCTTCTCCA 
AGCAAAACGT CCTTACCACT GTCTCCTGTG AAAACAGCTC CACTCTTTAG CAATGACGGG 
TTGAAAGATG GTTTGTGCTT GTCGGAATCA GAAACAGTCA ACAAAGAGCG AGCGAACAGT 
TACAAAAATC CCCGCACGCA GGACCTCACC GCCAAGCTTC GGAAAGCTGT GGAGAAGGGA 
GAGGAGGACA CCTTTTCTGA CCTTATCTGG AGCAACCCCC GGTATCTGAT AGGCTCAGGA 
GACAACCCCA CTATCGTGCA GGAAGGGTGC AGGTACAACG TGATGCATGT TGCTGCCAAA 
GAGAACCAGG CTTCCATCTG CCAGCTGACT CTGGACGTCC TGGAGAACCC TGACTTCATG 
AGGCTGATGT ACCCTGATGA CGACGAGGCC ATGCTGCAGA AGCGTATCCG TTACGTGGTG 
GACCTGTACC TCAACACCCC CGACAAGATG GGCTACGACA CACCGTTGCA TTTTGCTTGT 
AAGTTTGGAA ATGCAGATGT AGTCAACGTG CTTTCGTCAC ACCATTTGAT TGTAAAAAAC 
TCAAGGAATA AATATGATAA AACACCTGAA GATGTAATTT GTGAAAGAAG CAAAAATAAA 
TCTGTGGAAC TGAAGGAGCG GATCAGAGAG TATTTAAAGG GCCACTACTA CGTGCCCCTC 
CTGAGAGCGG AAGAGACTTC TTCTCCAGTC ATCGGGGAGC TGTGGTCCCC AGACCAGACG 
GCCGAGGCCT CTCACGTCAG CCACTATGGA GGCAGCCCCA GAGACCCGGT GCTGACCCTG 
AGAGCCTTCG CAGGGCCCCT GAGTCCAGCC AAGGCAGAAG ATTTTCGCAA GCTCTGGAAA 
ACTCCACCTC GAGAGAAAGC AGGCTTCCTT CACCACGTCA AGAAGTCGGA CCCGGAAAGA 
GGCTTTGAGA GAGTGGGAAG GGAGCTAGCT CATGAGCTGG GGTATCCCTG GGTTGAATAC 
TGGGAATTTC TGGGCTGTTT TGTTGATCTG TCTTCCCAGG AAGGCCTGCA AAGACTAGAA 
GAATATCTCA CACAGCAGGA AATAGGCAAA AAGGCTCAAC AAGAAACAGG AGAACGGGAA 
GCCTCCTGCC GAGATAAAGC CACCACGTCT GGAAAAAGAC GAGTGCACAT CAGAATACTG 
GTGTATATCA GACACCAGGG CACAAAGCGC CTCGCACGGT GCGTATCAGA CACCAGGACG 
CAGAGCGCTT CACACAGTGC ATATCAGACA CCAGGACGCA GAGAGCTTCA CACGGTGCAT 
TTCAGACACC AGGACGCAGA GCGCTTCACA CGGTGCATAT CAGACACCAG GACGGAGAGC 
GCTTCACACG GTGCATATCA GACACCAGGA CGCAGAGCGC TTCAGACAGT GCATATCAGA 
CACCAGGACG CAGAGCGCTT CACACGGTGC ATATCAGACA CCAGGACGCA GAGAGCTTCG 
CACGGTGCAT ATCAGACACT AGGACGCAGA GAGCTTCGCA CGGTGCGTAT CAGACACCAG 
GATGGAGAGC GCTTCACACG GTGCATATCA GACACCAGGA CGCAGAGCGC TTCACACGGT 
GCATATCAGA CACCAGGACG CAGAGCGCTT CAGACGGTGC ATATCAGACA CCAGGACGCA 
GAGAGCTTCG CACGGTGCAT ATCAGACACT AGGACGCAGA GCGCTTCGCA CGGTGCATAT 
CAGACACCAG GACGCAGAGC GCTTCGCACG GTGCGTATCA GACACCGGGA CGCAGAGAGC 
TTCGCACGGT GCGTATCAGA CACCGGGACG CAGAGAGCTT CGCACGGTGC GTATCAGACA 
CCGGGACGCA GAGAGCTTCA CACGGTGCGT ATCAGACACC GGGACGGAGA GCGCTTCACA 
CGGTGCGTAT CAGACACCGG GACGCAGAGC GCTTCAGACA GTGCATATCA GACACCAGGA 
CGCAGAGCGC TTCACACGGT GCATATCAGA CACCAGGACG CAGAGAGCTT CGCACGGTGC 
ATATCAGACA CTAGGACGCA GAGAGCTTCG CACGGTGCGT ATCAGACACC AGGATGGAGA 
GCGCTTCACA CGGTGCATAT CAGACACCAG GACGCAGAGC GCTTCACACG GTGCATATCA 
GACACCAGGA CGCAGAGCGC TTCAGACGGT GCATATCAGA CACCAGGACG CAGAGAGCTT 
CGCACGGTGC ATATCAGACA CCAGGACGCA GAGCGCTTCG CACGGTGCGT ATCAGACACC 
GGGACGCAGA GAGCTTCACA CGGTGCGTAT CAGACACCGG GACGGAGAGA GCTTCGCACG 
GTGCGTATCA GACACCGGGA CGCAGAGCGC TTCACACGGT GCATATCAGA CACCAGGACG 
CAAACACAAA GCACTTCACA CGGTGTATTT CAAACACCAG GACGCAAAGT GCTTCCAAAG 
CACAGGTGCA CTGAATGGAA AGATCATGAT GGAAGAGGTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANKLE2 ( XM_024348186.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_024203954.1 Pan troglodytes ankyrin repeat and LEM domain containing 2 (ANKLE2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OPc96704
Clone ID Related Accession (Same CDS sequence)	XM_024348186.1 , XM_024348185.1
Accession Version	XM_024348186.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3036bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	ankyrin repeat and LEM domain-containing protein 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036891.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACAATGG ATGCTCTGTT GGCTCGATTG AAACTTCTGA ATCCAGATGA CCTTAGAGAA 
GAAATCGTCA AAGCTGGATT GAAATGTGGA CCCATTACAT CAACTACAAG GTTCATTTTT 
GAGAAAAAAT TGGCTCAGGC TTTACTGGAG CAAGGAGGAA GGCTGTCTTC TTTCTACCAC 
CATGAGGCAG GTGTCACAAC TCTCAGCCAA GACCCACAAA GGATTTTGAA GCCAGCTGAA 
GGGAACCCAA CTGATCAGGC TGGTTTTTCT GAAGACAGAG ATTTTGGTTA CAGTGTGGGC 
CTGAATCCTC CAGAGGAGGA AGCTGTGACA TCCAAGACCT GCTCGGTGCC CCCTAGTGCC 
ACCGACACCT ACAGAGCTGG AGCGACTGCG TCTAAGGAGC CGCCCCTGTA CTATGGGGTG 
TGTCCAGTGT ATGAGGACGT CCCAGCGAGA AATGAAAGGA TCTATGTTTA TGAAAATAAA 
AAGGAAGCAT TGCAAGCTGT CAAGATGATC AAAGGGTCCC GATTTAAAGC TTTTTCTACC 
AGAGAAGACG CTGAGAAATT TGCTAGAGGA ATTTGTGATT ATTTCCCTTC TCCAAGCAAA 
ACGTCCTTAC CACTGTCTCC TGTGAAAACA GCTCCACTCT TTAGCAATGA CGGGTTGAAA 
GATGGTTTGT GCTTGTCGGA ATCAGAAACA GTCAACAAAG AGCGAGCGAA CAGTTACAAA 
AATCCCCGCA CGCAGGACCT CACCGCCAAG CTTCGGAAAG CTGTGGAGAA GGGAGAGGAG 
GACACCTTTT CTGACCTTAT CTGGAGCAAC CCCCGGTATC TGATAGGCTC AGGAGACAAC 
CCCACTATCG TGCAGGAAGG GTGCAGGTAC AACGTGATGC ATGTTGCTGC CAAAGAGAAC 
CAGGCTTCCA TCTGCCAGCT GACTCTGGAC GTCCTGGAGA ACCCTGACTT CATGAGGCTG 
ATGTACCCTG ATGACGACGA GGCCATGCTG CAGAAGCGTA TCCGTTACGT GGTGGACCTG 
TACCTCAACA CCCCCGACAA GATGGGCTAC GACACACCGT TGCATTTTGC TTGTAAGTTT 
GGAAATGCAG ATGTAGTCAA CGTGCTTTCG TCACACCATT TGATTGTAAA AAACTCAAGG 
AATAAATATG ATAAAACACC TGAAGATGTA ATTTGTGAAA GAAGCAAAAA TAAATCTGTG 
GAACTGAAGG AGCGGATCAG AGAGTATTTA AAGGGCCACT ACTACGTGCC CCTCCTGAGA 
GCGGAAGAGA CTTCTTCTCC AGTCATCGGG GAGCTGTGGT CCCCAGACCA GACGGCCGAG 
GCCTCTCACG TCAGCCACTA TGGAGGCAGC CCCAGAGACC CGGTGCTGAC CCTGAGAGCC 
TTCGCAGGGC CCCTGAGTCC AGCCAAGGCA GAAGATTTTC GCAAGCTCTG GAAAACTCCA 
CCTCGAGAGA AAGCAGGCTT CCTTCACCAC GTCAAGAAGT CGGACCCGGA AAGAGGCTTT 
GAGAGAGTGG GAAGGGAGCT AGCTCATGAG CTGGGGTATC CCTGGGTTGA ATACTGGGAA 
TTTCTGGGCT GTTTTGTTGA TCTGTCTTCC CAGGAAGGCC TGCAAAGACT AGAAGAATAT 
CTCACACAGC AGGAAATAGG CAAAAAGGCT CAACAAGAAA CAGGAGAACG GGAAGCCTCC 
TGCCGAGATA AAGCCACCAC GTCTGGAAAA AGACGAGTGC ACATCAGAAT ACTGGTGTAT 
ATCAGACACC AGGGCACAAA GCGCCTCGCA CGGTGCGTAT CAGACACCAG GACGCAGAGC 
GCTTCACACA GTGCATATCA GACACCAGGA CGCAGAGAGC TTCACACGGT GCATTTCAGA 
CACCAGGACG CAGAGCGCTT CACACGGTGC ATATCAGACA CCAGGACGGA GAGCGCTTCA 
CACGGTGCAT ATCAGACACC AGGACGCAGA GCGCTTCAGA CAGTGCATAT CAGACACCAG 
GACGCAGAGC GCTTCACACG GTGCATATCA GACACCAGGA CGCAGAGAGC TTCGCACGGT 
GCATATCAGA CACTAGGACG CAGAGAGCTT CGCACGGTGC GTATCAGACA CCAGGATGGA 
GAGCGCTTCA CACGGTGCAT ATCAGACACC AGGACGCAGA GCGCTTCACA CGGTGCATAT 
CAGACACCAG GACGCAGAGC GCTTCAGACG GTGCATATCA GACACCAGGA CGCAGAGAGC 
TTCGCACGGT GCATATCAGA CACTAGGACG CAGAGCGCTT CGCACGGTGC ATATCAGACA 
CCAGGACGCA GAGCGCTTCG CACGGTGCGT ATCAGACACC GGGACGCAGA GAGCTTCGCA 
CGGTGCGTAT CAGACACCGG GACGCAGAGA GCTTCGCACG GTGCGTATCA GACACCGGGA 
CGCAGAGAGC TTCACACGGT GCGTATCAGA CACCGGGACG GAGAGCGCTT CACACGGTGC 
GTATCAGACA CCGGGACGCA GAGCGCTTCA GACAGTGCAT ATCAGACACC AGGACGCAGA 
GCGCTTCACA CGGTGCATAT CAGACACCAG GACGCAGAGA GCTTCGCACG GTGCATATCA 
GACACTAGGA CGCAGAGAGC TTCGCACGGT GCGTATCAGA CACCAGGATG GAGAGCGCTT 
CACACGGTGC ATATCAGACA CCAGGACGCA GAGCGCTTCA CACGGTGCAT ATCAGACACC 
AGGACGCAGA GCGCTTCAGA CGGTGCATAT CAGACACCAG GACGCAGAGA GCTTCGCACG 
GTGCATATCA GACACCAGGA CGCAGAGCGC TTCGCACGGT GCGTATCAGA CACCGGGACG 
CAGAGAGCTT CACACGGTGC GTATCAGACA CCGGGACGGA GAGAGCTTCG CACGGTGCGT 
ATCAGACACC GGGACGCAGA GCGCTTCACA CGGTGCATAT CAGACACCAG GACGCAAACA 
CAAAGCACTT CACACGGTGT ATTTCAAACA CCAGGACGCA AAGTGCTTCC AAAGCACAGG 
TGCACTGAAT GGAAAGATCA TGATGGAAGA GGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024203954.1
CDS	137..3172
Translation	MTMDALLARLKLLNPDDLREEIVKAGLKCGPITSTTRFIFEKKLAQALLEQGGRLSSFYHHEAGVTTLSQDPQRILKPAEGNPTDQAGFSEDRDFGYSVGLNPPEEEAVTSKTCSVPPSATDTYRAGATASKEPPLYYGVCPVYEDVPARNERIYVYENKKEALQAVKMIKGSRFKAFSTREDAEKFARGICDYFPSPSKTSLPLSPVKTAPLFSNDGLKDGLCLSESETVNKERANSYKNPRTQDLTAKLRKAVEKGEEDTFSDLIWSNPRYLIGSGDNPTIVQEGCRYNVMHVAAKENQASICQLTLDVLENPDFMRLMYPDDDEAMLQKRIRYVVDLYLNTPDKMGYDTPLHFACKFGNADVVNVLSSHHLIVKNSRNKYDKTPEDVICERSKNKSVELKERIREYLKGHYYVPLLRAEETSSPVIGELWSPDQTAEASHVSHYGGSPRDPVLTLRAFAGPLSPAKAEDFRKLWKTPPREKAGFLHHVKKSDPERGFERVGRELAHELGYPWVEYWEFLGCFVDLSSQEGLQRLEEYLTQQEIGKKAQQETGEREASCRDKATTSGKRRVHIRILVYIRHQGTKRLARCVSDTRTQSASHSAYQTPGRRELHTVHFRHQDAERFTRCISDTRTESASHGAYQTPGRRALQTVHIRHQDAERFTRCISDTRTQRASHGAYQTLGRRELRTVRIRHQDGERFTRCISDTRTQSASHGAYQTPGRRALQTVHIRHQDAESFARCISDTRTQSASHGAYQTPGRRALRTVRIRHRDAESFARCVSDTGTQRASHGAYQTPGRRELHTVRIRHRDGERFTRCVSDTGTQSASDSAYQTPGRRALHTVHIRHQDAESFARCISDTRTQRASHGAYQTPGWRALHTVHIRHQDAERFTRCISDTRTQSASDGAYQTPGRRELRTVHIRHQDAERFARCVSDTGTQRASHGAYQTPGRRELRTVRIRHRDAERFTRCISDTRTQTQSTSHGVFQTPGRKVLPKHRCTEWKDHDGRG

Target ORF information:

RefSeq Version	XM_024348186.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes ankyrin repeat and LEM domain containing 2 (ANKLE2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024348186.1

ORF Insert Sequence:

ATGACAATGG ATGCTCTGTT GGCTCGATTG AAACTTCTGA ATCCAGATGA CCTTAGAGAA 
GAAATCGTCA AAGCTGGATT GAAATGTGGA CCCATTACAT CAACTACAAG GTTCATTTTT 
GAGAAAAAAT TGGCTCAGGC TTTACTGGAG CAAGGAGGAA GGCTGTCTTC TTTCTACCAC 
CATGAGGCAG GTGTCACAAC TCTCAGCCAA GACCCACAAA GGATTTTGAA GCCAGCTGAA 
GGGAACCCAA CTGATCAGGC TGGTTTTTCT GAAGACAGAG ATTTTGGTTA CAGTGTGGGC 
CTGAATCCTC CAGAGGAGGA AGCTGTGACA TCCAAGACCT GCTCGGTGCC CCCTAGTGCC 
ACCGACACCT ACAGAGCTGG AGCGACTGCG TCTAAGGAGC CGCCCCTGTA CTATGGGGTG 
TGTCCAGTGT ATGAGGACGT CCCAGCGAGA AATGAAAGGA TCTATGTTTA TGAAAATAAA 
AAGGAAGCAT TGCAAGCTGT CAAGATGATC AAAGGGTCCC GATTTAAAGC TTTTTCTACC 
AGAGAAGACG CTGAGAAATT TGCTAGAGGA ATTTGTGATT ATTTCCCTTC TCCAAGCAAA 
ACGTCCTTAC CACTGTCTCC TGTGAAAACA GCTCCACTCT TTAGCAATGA CGGGTTGAAA 
GATGGTTTGT GCTTGTCGGA ATCAGAAACA GTCAACAAAG AGCGAGCGAA CAGTTACAAA 
AATCCCCGCA CGCAGGACCT CACCGCCAAG CTTCGGAAAG CTGTGGAGAA GGGAGAGGAG 
GACACCTTTT CTGACCTTAT CTGGAGCAAC CCCCGGTATC TGATAGGCTC AGGAGACAAC 
CCCACTATCG TGCAGGAAGG GTGCAGGTAC AACGTGATGC ATGTTGCTGC CAAAGAGAAC 
CAGGCTTCCA TCTGCCAGCT GACTCTGGAC GTCCTGGAGA ACCCTGACTT CATGAGGCTG 
ATGTACCCTG ATGACGACGA GGCCATGCTG CAGAAGCGTA TCCGTTACGT GGTGGACCTG 
TACCTCAACA CCCCCGACAA GATGGGCTAC GACACACCGT TGCATTTTGC TTGTAAGTTT 
GGAAATGCAG ATGTAGTCAA CGTGCTTTCG TCACACCATT TGATTGTAAA AAACTCAAGG 
AATAAATATG ATAAAACACC TGAAGATGTA ATTTGTGAAA GAAGCAAAAA TAAATCTGTG 
GAACTGAAGG AGCGGATCAG AGAGTATTTA AAGGGCCACT ACTACGTGCC CCTCCTGAGA 
GCGGAAGAGA CTTCTTCTCC AGTCATCGGG GAGCTGTGGT CCCCAGACCA GACGGCCGAG 
GCCTCTCACG TCAGCCACTA TGGAGGCAGC CCCAGAGACC CGGTGCTGAC CCTGAGAGCC 
TTCGCAGGGC CCCTGAGTCC AGCCAAGGCA GAAGATTTTC GCAAGCTCTG GAAAACTCCA 
CCTCGAGAGA AAGCAGGCTT CCTTCACCAC GTCAAGAAGT CGGACCCGGA AAGAGGCTTT 
GAGAGAGTGG GAAGGGAGCT AGCTCATGAG CTGGGGTATC CCTGGGTTGA ATACTGGGAA 
TTTCTGGGCT GTTTTGTTGA TCTGTCTTCC CAGGAAGGCC TGCAAAGACT AGAAGAATAT 
CTCACACAGC AGGAAATAGG CAAAAAGGCT CAACAAGAAA CAGGAGAACG GGAAGCCTCC 
TGCCGAGATA AAGCCACCAC GTCTGGAAAA AGACGAGTGC ACATCAGAAT ACTGGTGTAT 
ATCAGACACC AGGGCACAAA GCGCCTCGCA CGGTGCGTAT CAGACACCAG GACGCAGAGC 
GCTTCACACA GTGCATATCA GACACCAGGA CGCAGAGAGC TTCACACGGT GCATTTCAGA 
CACCAGGACG CAGAGCGCTT CACACGGTGC ATATCAGACA CCAGGACGGA GAGCGCTTCA 
CACGGTGCAT ATCAGACACC AGGACGCAGA GCGCTTCAGA CAGTGCATAT CAGACACCAG 
GACGCAGAGC GCTTCACACG GTGCATATCA GACACCAGGA CGCAGAGAGC TTCGCACGGT 
GCATATCAGA CACTAGGACG CAGAGAGCTT CGCACGGTGC GTATCAGACA CCAGGATGGA 
GAGCGCTTCA CACGGTGCAT ATCAGACACC AGGACGCAGA GCGCTTCACA CGGTGCATAT 
CAGACACCAG GACGCAGAGC GCTTCAGACG GTGCATATCA GACACCAGGA CGCAGAGAGC 
TTCGCACGGT GCATATCAGA CACTAGGACG CAGAGCGCTT CGCACGGTGC ATATCAGACA 
CCAGGACGCA GAGCGCTTCG CACGGTGCGT ATCAGACACC GGGACGCAGA GAGCTTCGCA 
CGGTGCGTAT CAGACACCGG GACGCAGAGA GCTTCGCACG GTGCGTATCA GACACCGGGA 
CGCAGAGAGC TTCACACGGT GCGTATCAGA CACCGGGACG GAGAGCGCTT CACACGGTGC 
GTATCAGACA CCGGGACGCA GAGCGCTTCA GACAGTGCAT ATCAGACACC AGGACGCAGA 
GCGCTTCACA CGGTGCATAT CAGACACCAG GACGCAGAGA GCTTCGCACG GTGCATATCA 
GACACTAGGA CGCAGAGAGC TTCGCACGGT GCGTATCAGA CACCAGGATG GAGAGCGCTT 
CACACGGTGC ATATCAGACA CCAGGACGCA GAGCGCTTCA CACGGTGCAT ATCAGACACC 
AGGACGCAGA GCGCTTCAGA CGGTGCATAT CAGACACCAG GACGCAGAGA GCTTCGCACG 
GTGCATATCA GACACCAGGA CGCAGAGCGC TTCGCACGGT GCGTATCAGA CACCGGGACG 
CAGAGAGCTT CACACGGTGC GTATCAGACA CCGGGACGGA GAGAGCTTCG CACGGTGCGT 
ATCAGACACC GGGACGCAGA GCGCTTCACA CGGTGCATAT CAGACACCAG GACGCAAACA 
CAAAGCACTT CACACGGTGT ATTTCAAACA CCAGGACGCA AAGTGCTTCC AAAGCACAGG 
TGCACTGAAT GGAAAGATCA TGATGGAAGA GGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANKLE2 ( XM_024348185.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_024203953.1 Pan troglodytes ankyrin repeat and LEM domain containing 2 (ANKLE2), transcript variant X2, mRNA.

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CloneID	OPc96704
Clone ID Related Accession (Same CDS sequence)	XM_024348186.1 , XM_024348185.1
Accession Version	XM_024348185.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3036bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	ankyrin repeat and LEM domain-containing protein 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036891.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACAATGG ATGCTCTGTT GGCTCGATTG AAACTTCTGA ATCCAGATGA CCTTAGAGAA 
GAAATCGTCA AAGCTGGATT GAAATGTGGA CCCATTACAT CAACTACAAG GTTCATTTTT 
GAGAAAAAAT TGGCTCAGGC TTTACTGGAG CAAGGAGGAA GGCTGTCTTC TTTCTACCAC 
CATGAGGCAG GTGTCACAAC TCTCAGCCAA GACCCACAAA GGATTTTGAA GCCAGCTGAA 
GGGAACCCAA CTGATCAGGC TGGTTTTTCT GAAGACAGAG ATTTTGGTTA CAGTGTGGGC 
CTGAATCCTC CAGAGGAGGA AGCTGTGACA TCCAAGACCT GCTCGGTGCC CCCTAGTGCC 
ACCGACACCT ACAGAGCTGG AGCGACTGCG TCTAAGGAGC CGCCCCTGTA CTATGGGGTG 
TGTCCAGTGT ATGAGGACGT CCCAGCGAGA AATGAAAGGA TCTATGTTTA TGAAAATAAA 
AAGGAAGCAT TGCAAGCTGT CAAGATGATC AAAGGGTCCC GATTTAAAGC TTTTTCTACC 
AGAGAAGACG CTGAGAAATT TGCTAGAGGA ATTTGTGATT ATTTCCCTTC TCCAAGCAAA 
ACGTCCTTAC CACTGTCTCC TGTGAAAACA GCTCCACTCT TTAGCAATGA CGGGTTGAAA 
GATGGTTTGT GCTTGTCGGA ATCAGAAACA GTCAACAAAG AGCGAGCGAA CAGTTACAAA 
AATCCCCGCA CGCAGGACCT CACCGCCAAG CTTCGGAAAG CTGTGGAGAA GGGAGAGGAG 
GACACCTTTT CTGACCTTAT CTGGAGCAAC CCCCGGTATC TGATAGGCTC AGGAGACAAC 
CCCACTATCG TGCAGGAAGG GTGCAGGTAC AACGTGATGC ATGTTGCTGC CAAAGAGAAC 
CAGGCTTCCA TCTGCCAGCT GACTCTGGAC GTCCTGGAGA ACCCTGACTT CATGAGGCTG 
ATGTACCCTG ATGACGACGA GGCCATGCTG CAGAAGCGTA TCCGTTACGT GGTGGACCTG 
TACCTCAACA CCCCCGACAA GATGGGCTAC GACACACCGT TGCATTTTGC TTGTAAGTTT 
GGAAATGCAG ATGTAGTCAA CGTGCTTTCG TCACACCATT TGATTGTAAA AAACTCAAGG 
AATAAATATG ATAAAACACC TGAAGATGTA ATTTGTGAAA GAAGCAAAAA TAAATCTGTG 
GAACTGAAGG AGCGGATCAG AGAGTATTTA AAGGGCCACT ACTACGTGCC CCTCCTGAGA 
GCGGAAGAGA CTTCTTCTCC AGTCATCGGG GAGCTGTGGT CCCCAGACCA GACGGCCGAG 
GCCTCTCACG TCAGCCACTA TGGAGGCAGC CCCAGAGACC CGGTGCTGAC CCTGAGAGCC 
TTCGCAGGGC CCCTGAGTCC AGCCAAGGCA GAAGATTTTC GCAAGCTCTG GAAAACTCCA 
CCTCGAGAGA AAGCAGGCTT CCTTCACCAC GTCAAGAAGT CGGACCCGGA AAGAGGCTTT 
GAGAGAGTGG GAAGGGAGCT AGCTCATGAG CTGGGGTATC CCTGGGTTGA ATACTGGGAA 
TTTCTGGGCT GTTTTGTTGA TCTGTCTTCC CAGGAAGGCC TGCAAAGACT AGAAGAATAT 
CTCACACAGC AGGAAATAGG CAAAAAGGCT CAACAAGAAA CAGGAGAACG GGAAGCCTCC 
TGCCGAGATA AAGCCACCAC GTCTGGAAAA AGACGAGTGC ACATCAGAAT ACTGGTGTAT 
ATCAGACACC AGGGCACAAA GCGCCTCGCA CGGTGCGTAT CAGACACCAG GACGCAGAGC 
GCTTCACACA GTGCATATCA GACACCAGGA CGCAGAGAGC TTCACACGGT GCATTTCAGA 
CACCAGGACG CAGAGCGCTT CACACGGTGC ATATCAGACA CCAGGACGGA GAGCGCTTCA 
CACGGTGCAT ATCAGACACC AGGACGCAGA GCGCTTCAGA CAGTGCATAT CAGACACCAG 
GACGCAGAGC GCTTCACACG GTGCATATCA GACACCAGGA CGCAGAGAGC TTCGCACGGT 
GCATATCAGA CACTAGGACG CAGAGAGCTT CGCACGGTGC GTATCAGACA CCAGGATGGA 
GAGCGCTTCA CACGGTGCAT ATCAGACACC AGGACGCAGA GCGCTTCACA CGGTGCATAT 
CAGACACCAG GACGCAGAGC GCTTCAGACG GTGCATATCA GACACCAGGA CGCAGAGAGC 
TTCGCACGGT GCATATCAGA CACTAGGACG CAGAGCGCTT CGCACGGTGC ATATCAGACA 
CCAGGACGCA GAGCGCTTCG CACGGTGCGT ATCAGACACC GGGACGCAGA GAGCTTCGCA 
CGGTGCGTAT CAGACACCGG GACGCAGAGA GCTTCGCACG GTGCGTATCA GACACCGGGA 
CGCAGAGAGC TTCACACGGT GCGTATCAGA CACCGGGACG GAGAGCGCTT CACACGGTGC 
GTATCAGACA CCGGGACGCA GAGCGCTTCA GACAGTGCAT ATCAGACACC AGGACGCAGA 
GCGCTTCACA CGGTGCATAT CAGACACCAG GACGCAGAGA GCTTCGCACG GTGCATATCA 
GACACTAGGA CGCAGAGAGC TTCGCACGGT GCGTATCAGA CACCAGGATG GAGAGCGCTT 
CACACGGTGC ATATCAGACA CCAGGACGCA GAGCGCTTCA CACGGTGCAT ATCAGACACC 
AGGACGCAGA GCGCTTCAGA CGGTGCATAT CAGACACCAG GACGCAGAGA GCTTCGCACG 
GTGCATATCA GACACCAGGA CGCAGAGCGC TTCGCACGGT GCGTATCAGA CACCGGGACG 
CAGAGAGCTT CACACGGTGC GTATCAGACA CCGGGACGGA GAGAGCTTCG CACGGTGCGT 
ATCAGACACC GGGACGCAGA GCGCTTCACA CGGTGCATAT CAGACACCAG GACGCAAACA 
CAAAGCACTT CACACGGTGT ATTTCAAACA CCAGGACGCA AAGTGCTTCC AAAGCACAGG 
TGCACTGAAT GGAAAGATCA TGATGGAAGA GGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024203953.1
CDS	337..3372
Translation	MTMDALLARLKLLNPDDLREEIVKAGLKCGPITSTTRFIFEKKLAQALLEQGGRLSSFYHHEAGVTTLSQDPQRILKPAEGNPTDQAGFSEDRDFGYSVGLNPPEEEAVTSKTCSVPPSATDTYRAGATASKEPPLYYGVCPVYEDVPARNERIYVYENKKEALQAVKMIKGSRFKAFSTREDAEKFARGICDYFPSPSKTSLPLSPVKTAPLFSNDGLKDGLCLSESETVNKERANSYKNPRTQDLTAKLRKAVEKGEEDTFSDLIWSNPRYLIGSGDNPTIVQEGCRYNVMHVAAKENQASICQLTLDVLENPDFMRLMYPDDDEAMLQKRIRYVVDLYLNTPDKMGYDTPLHFACKFGNADVVNVLSSHHLIVKNSRNKYDKTPEDVICERSKNKSVELKERIREYLKGHYYVPLLRAEETSSPVIGELWSPDQTAEASHVSHYGGSPRDPVLTLRAFAGPLSPAKAEDFRKLWKTPPREKAGFLHHVKKSDPERGFERVGRELAHELGYPWVEYWEFLGCFVDLSSQEGLQRLEEYLTQQEIGKKAQQETGEREASCRDKATTSGKRRVHIRILVYIRHQGTKRLARCVSDTRTQSASHSAYQTPGRRELHTVHFRHQDAERFTRCISDTRTESASHGAYQTPGRRALQTVHIRHQDAERFTRCISDTRTQRASHGAYQTLGRRELRTVRIRHQDGERFTRCISDTRTQSASHGAYQTPGRRALQTVHIRHQDAESFARCISDTRTQSASHGAYQTPGRRALRTVRIRHRDAESFARCVSDTGTQRASHGAYQTPGRRELHTVRIRHRDGERFTRCVSDTGTQSASDSAYQTPGRRALHTVHIRHQDAESFARCISDTRTQRASHGAYQTPGWRALHTVHIRHQDAERFTRCISDTRTQSASDGAYQTPGRRELRTVHIRHQDAERFARCVSDTGTQRASHGAYQTPGRRELRTVRIRHRDAERFTRCISDTRTQTQSTSHGVFQTPGRKVLPKHRCTEWKDHDGRG

Target ORF information:

RefSeq Version	XM_024348185.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes ankyrin repeat and LEM domain containing 2 (ANKLE2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024348185.1

ORF Insert Sequence:

ATGACAATGG ATGCTCTGTT GGCTCGATTG AAACTTCTGA ATCCAGATGA CCTTAGAGAA 
GAAATCGTCA AAGCTGGATT GAAATGTGGA CCCATTACAT CAACTACAAG GTTCATTTTT 
GAGAAAAAAT TGGCTCAGGC TTTACTGGAG CAAGGAGGAA GGCTGTCTTC TTTCTACCAC 
CATGAGGCAG GTGTCACAAC TCTCAGCCAA GACCCACAAA GGATTTTGAA GCCAGCTGAA 
GGGAACCCAA CTGATCAGGC TGGTTTTTCT GAAGACAGAG ATTTTGGTTA CAGTGTGGGC 
CTGAATCCTC CAGAGGAGGA AGCTGTGACA TCCAAGACCT GCTCGGTGCC CCCTAGTGCC 
ACCGACACCT ACAGAGCTGG AGCGACTGCG TCTAAGGAGC CGCCCCTGTA CTATGGGGTG 
TGTCCAGTGT ATGAGGACGT CCCAGCGAGA AATGAAAGGA TCTATGTTTA TGAAAATAAA 
AAGGAAGCAT TGCAAGCTGT CAAGATGATC AAAGGGTCCC GATTTAAAGC TTTTTCTACC 
AGAGAAGACG CTGAGAAATT TGCTAGAGGA ATTTGTGATT ATTTCCCTTC TCCAAGCAAA 
ACGTCCTTAC CACTGTCTCC TGTGAAAACA GCTCCACTCT TTAGCAATGA CGGGTTGAAA 
GATGGTTTGT GCTTGTCGGA ATCAGAAACA GTCAACAAAG AGCGAGCGAA CAGTTACAAA 
AATCCCCGCA CGCAGGACCT CACCGCCAAG CTTCGGAAAG CTGTGGAGAA GGGAGAGGAG 
GACACCTTTT CTGACCTTAT CTGGAGCAAC CCCCGGTATC TGATAGGCTC AGGAGACAAC 
CCCACTATCG TGCAGGAAGG GTGCAGGTAC AACGTGATGC ATGTTGCTGC CAAAGAGAAC 
CAGGCTTCCA TCTGCCAGCT GACTCTGGAC GTCCTGGAGA ACCCTGACTT CATGAGGCTG 
ATGTACCCTG ATGACGACGA GGCCATGCTG CAGAAGCGTA TCCGTTACGT GGTGGACCTG 
TACCTCAACA CCCCCGACAA GATGGGCTAC GACACACCGT TGCATTTTGC TTGTAAGTTT 
GGAAATGCAG ATGTAGTCAA CGTGCTTTCG TCACACCATT TGATTGTAAA AAACTCAAGG 
AATAAATATG ATAAAACACC TGAAGATGTA ATTTGTGAAA GAAGCAAAAA TAAATCTGTG 
GAACTGAAGG AGCGGATCAG AGAGTATTTA AAGGGCCACT ACTACGTGCC CCTCCTGAGA 
GCGGAAGAGA CTTCTTCTCC AGTCATCGGG GAGCTGTGGT CCCCAGACCA GACGGCCGAG 
GCCTCTCACG TCAGCCACTA TGGAGGCAGC CCCAGAGACC CGGTGCTGAC CCTGAGAGCC 
TTCGCAGGGC CCCTGAGTCC AGCCAAGGCA GAAGATTTTC GCAAGCTCTG GAAAACTCCA 
CCTCGAGAGA AAGCAGGCTT CCTTCACCAC GTCAAGAAGT CGGACCCGGA AAGAGGCTTT 
GAGAGAGTGG GAAGGGAGCT AGCTCATGAG CTGGGGTATC CCTGGGTTGA ATACTGGGAA 
TTTCTGGGCT GTTTTGTTGA TCTGTCTTCC CAGGAAGGCC TGCAAAGACT AGAAGAATAT 
CTCACACAGC AGGAAATAGG CAAAAAGGCT CAACAAGAAA CAGGAGAACG GGAAGCCTCC 
TGCCGAGATA AAGCCACCAC GTCTGGAAAA AGACGAGTGC ACATCAGAAT ACTGGTGTAT 
ATCAGACACC AGGGCACAAA GCGCCTCGCA CGGTGCGTAT CAGACACCAG GACGCAGAGC 
GCTTCACACA GTGCATATCA GACACCAGGA CGCAGAGAGC TTCACACGGT GCATTTCAGA 
CACCAGGACG CAGAGCGCTT CACACGGTGC ATATCAGACA CCAGGACGGA GAGCGCTTCA 
CACGGTGCAT ATCAGACACC AGGACGCAGA GCGCTTCAGA CAGTGCATAT CAGACACCAG 
GACGCAGAGC GCTTCACACG GTGCATATCA GACACCAGGA CGCAGAGAGC TTCGCACGGT 
GCATATCAGA CACTAGGACG CAGAGAGCTT CGCACGGTGC GTATCAGACA CCAGGATGGA 
GAGCGCTTCA CACGGTGCAT ATCAGACACC AGGACGCAGA GCGCTTCACA CGGTGCATAT 
CAGACACCAG GACGCAGAGC GCTTCAGACG GTGCATATCA GACACCAGGA CGCAGAGAGC 
TTCGCACGGT GCATATCAGA CACTAGGACG CAGAGCGCTT CGCACGGTGC ATATCAGACA 
CCAGGACGCA GAGCGCTTCG CACGGTGCGT ATCAGACACC GGGACGCAGA GAGCTTCGCA 
CGGTGCGTAT CAGACACCGG GACGCAGAGA GCTTCGCACG GTGCGTATCA GACACCGGGA 
CGCAGAGAGC TTCACACGGT GCGTATCAGA CACCGGGACG GAGAGCGCTT CACACGGTGC 
GTATCAGACA CCGGGACGCA GAGCGCTTCA GACAGTGCAT ATCAGACACC AGGACGCAGA 
GCGCTTCACA CGGTGCATAT CAGACACCAG GACGCAGAGA GCTTCGCACG GTGCATATCA 
GACACTAGGA CGCAGAGAGC TTCGCACGGT GCGTATCAGA CACCAGGATG GAGAGCGCTT 
CACACGGTGC ATATCAGACA CCAGGACGCA GAGCGCTTCA CACGGTGCAT ATCAGACACC 
AGGACGCAGA GCGCTTCAGA CGGTGCATAT CAGACACCAG GACGCAGAGA GCTTCGCACG 
GTGCATATCA GACACCAGGA CGCAGAGCGC TTCGCACGGT GCGTATCAGA CACCGGGACG 
CAGAGAGCTT CACACGGTGC GTATCAGACA CCGGGACGGA GAGAGCTTCG CACGGTGCGT 
ATCAGACACC GGGACGCAGA GCGCTTCACA CGGTGCATAT CAGACACCAG GACGCAAACA 
CAAAGCACTT CACACGGTGT ATTTCAAACA CCAGGACGCA AAGTGCTTCC AAAGCACAGG 
TGCACTGAAT GGAAAGATCA TGATGGAAGA GGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Major chimpanzee-specific structural changes in sperm development-associated genes.
Functional & integrative genomics11(3)507-17(2011 Sep)
Kim RN,Kim DW,Choi SH,Chae SH,Nam SH,Kim DW,Kim A,Kang A,Park KH,Lee YS,Hirai M,Suzuki Y,Sugano S,Hashimoto K,Kim DS,Park HS

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ANKLE2 cDNA ORF clone, Pan troglodytes(chimpanzee)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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