VARS2 cDNA ORF clone, Pan troglodytes(chimpanzee)

Gene
Clones
gRNAs

Summary

Gene Symbol	VARS2
Entrez Gene ID	107975445
Full Name	valyl-tRNA synthetase 2, mitochondrial
Synonyms	VARS2L,VARSL
Gene Type	protein-coding
Organism	Pan troglodytes(chimpanzee)

mRNA and Protein(s)

mRNA	Protein	Name
XM_016956717.1	XP_016812206.1	valine--tRNA ligase, mitochondrial isoform X2
XM_016956719.2	XP_016812208.1	valine--tRNA ligase, mitochondrial isoform X3
XM_016956719.1	XP_016812208.1	valine--tRNA ligase, mitochondrial isoform X3
XM_016956716.2	XP_016812205.1	valine--tRNA ligase, mitochondrial isoform X2
XM_016956716.1	XP_016812205.1	valine--tRNA ligase, mitochondrial isoform X2
XM_016956713.1	XP_016812202.1	valine--tRNA ligase, mitochondrial isoform X1
XM_016956718.1	XP_016812207.1	valine--tRNA ligase, mitochondrial isoform X2

Pathways from BioSystems
Gene Ontology

The following VARS2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the VARS2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OPc87418	XM_016956719.2 Latest version!	Pan troglodytes valyl-tRNA synthetase 2, mitochondrial (VARS2), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OPc87416	XM_016956713.1 Latest version!	Pan troglodytes valyl-tRNA synthetase 2, mitochondrial (VARS2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OPc87417	XM_016956717.1 Latest version!	Pan troglodytes valyl-tRNA synthetase 2, mitochondrial (VARS2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OPc87417	XM_016956718.1 Latest version!	Pan troglodytes valyl-tRNA synthetase 2, mitochondrial (VARS2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OPc87417	XM_016956716.2 Latest version!	Pan troglodytes valyl-tRNA synthetase 2, mitochondrial (VARS2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
View Old Accession Versions >>
OPc87418	XM_016956719.1	Pan troglodytes valyl-tRNA synthetase 2, mitochondrial (VARS2), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OPc87417	XM_016956716.1	Pan troglodytes valyl-tRNA synthetase 2, mitochondrial (VARS2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

VARS2 ( XM_016956719.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016812208.1 Pan troglodytes valyl-tRNA synthetase 2, mitochondrial (VARS2), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OPc87418
Clone ID Related Accession (Same CDS sequence)	XM_016956719.2 , XM_016956719.1
Accession Version	XM_016956719.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2631bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	valine--tRNA ligase, mitochondrial isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036885.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_016956719.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCGGGA AGGCCTGGCC GCGCCGGGCT GTGGGCACTG CAGGAGGCCC CTGTGCAGAA 
CAGATCTCGG CCCCTTTCCA AACACTCCTG ATGCCTCATT TGCCTCTCGC CTCTTTTCGA 
CCACCATTTT GGGGGCTGAG GCACTCACGG GGCCTCCCCA GGTTTCACTC CGTTTCTACA 
CAGTCGGAGC CCCATGGATC TCCCATCTCC CGGAGGAACC GTGAAGCCAA ACAGAAGCGC 
CTGCGAGAGA AGCAGGCGAC TCTGGAGGCT GAGATAGCAG GGGAGAGCAA GTCACCTGCA 
GAATCCATTA AGGCCTGGAG GCCTAAGGAG TTAGTATTGT ATGAAATCCC TACGAAACCC 
GGTGAAAAGA AAGATGTCTC TGGGCCCCTG CCTCCTGCAT ACAGCCCCCG ATATGTTGAG 
GCTGCCTGGT ACCCGTGGTG GGTACGAGAG GGCTTCTTCA AACCAGAATA TCAGGCCCGG 
CTGCCCCAAG CTACAGGGGA GACCTTTTCC ATGTGTATCC CACCTCCCAA TGTCACTGGC 
TCCCTGCACA TTGGCCACGC ACTCACGGTG GCCATACAGG ATGCCCTCGT GCGCTGGCAC 
CGGATGCGTG GGGATCAAGT GCTGTGGGTC CCTGGTTCAG ATCATGCAGG AATTGCTACA 
CAAGCTGTGG TGGAGAAACA ACTGTGGAAG GAACGGGGAG TGAGGAGACA TGAGCTGAGC 
CGGGAGGCCT TCCTTAGGGA GGTGTGGCAG TGGAAGGAGG CGAAAGGTGG AGAGATCTGT 
GAGCAGCTGC GAGCTCTGGG TGCCTCCCTG GACTGGGATC GAGAGTGTTT TACCATGGAT 
GTTGGCTCCT CAGTGGCTGT GACTGAAGCT TTTGTGCAGC TCTACAAGGC GGGGTTGCTG 
TACCGGAACC ATCAGCTTGT CAACTGGTCA TGTGCTTTAA GATCAGCCAT CTCAGACATT 
GAGGTGGAGA ACCGGCCCCT GCCTGGCCAC ACACAGCTTC GACTGCCTGG CTGCCCCACC 
CCCGTGTCTT TTGGCCTCCT ATTTTCTATT GCCTTCCCGG TGGATGGAGA GCCTGATGCA 
GAGGTTGTGG TAGGAACCAC AAGGCCAGAG ACGCTGCCTG GAGATGTGGC TGTGGCCGTT 
CATCCAGACG ACTCGCGATA CACACATCTA CACGGGCGAC AGCTTCGTCA CCCCTTGATG 
GGGCAGCCTC TTCCCCTCAT CACAGACTAT GCTGTTCAGC CACATGTGGG CACGGGGGCA 
GTGAAGGTGA CTCCAGCTCA CAGTCCTGCC GATGCTGAGA TGGGGGCCCG ACATGGCTTG 
AGCCCCTTGA ATGTCATTGC GGAGGATGGG ACCATGACCT CCCTCTGCGG GGACTGGCTG 
CAGGGTCTTC ACCGGTTTGT GGCCCGGGAA AAGATAATGT CTGTGCTGAG TGAACGGGGC 
CTGTTCCGGG GCCTCCAGAA CCACCCCATG GTACTGCCCA TCTGCAGCCG TTCTGGGGAT 
GTGATAGAAT ACCTGCTGAA GAACCAGTGG TTTGTCCGCT GCCAGGAAAT GGGGGCCCGA 
GCTGCCAAGG CTGTGGAGTC GGGGGCCCTG GAGCTCAGTC CCTCCTTCCA CCAGAAGAAT 
TGGCAGCACT GGTTTTCCCA TATTGGGGAC TGGTGTGTCT CCCGGCAGCT GTGGTGGGGC 
CATCAGATTC CAGCCTACCT GGTTGTAGAG GACCATGCGC AGGGAGAAGA GGACTGTTGG 
GTGGTTGGGC GGTCAGAGGC TGAGGCCAGA GAGGTAGCAG CGGAACTGAC AGGGAGGCCA 
GGGGCAGAGC TGACCCTGGA GAGGGATCCT GATGTCCTAG ACACATGGTT TTCTTCTGCC 
CTGTTCCCCT TTTCTGCCCT GGGCTGGCCC CAAGAGACCC CAGACCTTGC TCGTTTCTAC 
CCCCTGTCAC TTTTGGAAAC GGGCAGTGAC CTTCTGCTGT TCTGGGTGGG CCGCATGGTC 
ATGTTGGGGA CCCAGCTCAC AGGGCAGCTG CCCTTCAGCA AGGTGCTTCT TCATCCCATG 
GTTCGGGACA GGCAGGGCCG GAAGATGAGC AAGTCCCTGG GGAATGTGCT GGACCCAAGA 
GACATCATCA GTGGGGTGGA GATGCAGGTG CTGCAGGAAA AGCTGAGAAG CGGAAATTTG 
GACCCTGCAG AGCTGGCCAT TGTGGCTGCA GCACAGAAAA AGGACTTTCC TCACGGGATC 
CCTGAGTGTG GGACAGATGC CCTGAGATTC ACACTCTGCT CCCATGGAGT TCAGGTGGGC 
GACTTGCACC TGTCAGTCTC TGAGGTCCAG AGCTGCCGAC ATTTCTGCAA CAAGATCTGG 
AATGCTCTTC GCTTTATCCT CAATGCTTTA GGGGAGAAAT TTGTGCCACA GCCTGCTGAG 
GAGCTGTCTC CCTCCTCCCC GATGGATGCC TGGATCTTGA GCCGCCTTGC CCTGGCTGCC 
CAGGAGTGTG AGCGGGGCTT CCTCACCCGA GAGCTCTCGC TCGTCACTCA TGCCCTGCAC 
CACTTCTGGC TTCACAACCT CTGTGACGTC TACCTGGACC CATGGCCTGC CCCGCTGGCA 
GGTAGCAGTG GCTGTAGGGA GGAGGGCTGT GGCCCTGGAC CTGTCCTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016812208.1
CDS	62..2692
Translation	MGGKAWPRRAVGTAGGPCAEQISAPFQTLLMPHLPLASFRPPFWGLRHSRGLPRFHSVSTQSEPHGSPISRRNREAKQKRLREKQATLEAEIAGESKSPAESIKAWRPKELVLYEIPTKPGEKKDVSGPLPPAYSPRYVEAAWYPWWVREGFFKPEYQARLPQATGETFSMCIPPPNVTGSLHIGHALTVAIQDALVRWHRMRGDQVLWVPGSDHAGIATQAVVEKQLWKERGVRRHELSREAFLREVWQWKEAKGGEICEQLRALGASLDWDRECFTMDVGSSVAVTEAFVQLYKAGLLYRNHQLVNWSCALRSAISDIEVENRPLPGHTQLRLPGCPTPVSFGLLFSIAFPVDGEPDAEVVVGTTRPETLPGDVAVAVHPDDSRYTHLHGRQLRHPLMGQPLPLITDYAVQPHVGTGAVKVTPAHSPADAEMGARHGLSPLNVIAEDGTMTSLCGDWLQGLHRFVAREKIMSVLSERGLFRGLQNHPMVLPICSRSGDVIEYLLKNQWFVRCQEMGARAAKAVESGALELSPSFHQKNWQHWFSHIGDWCVSRQLWWGHQIPAYLVVEDHAQGEEDCWVVGRSEAEAREVAAELTGRPGAELTLERDPDVLDTWFSSALFPFSALGWPQETPDLARFYPLSLLETGSDLLLFWVGRMVMLGTQLTGQLPFSKVLLHPMVRDRQGRKMSKSLGNVLDPRDIISGVEMQVLQEKLRSGNLDPAELAIVAAAQKKDFPHGIPECGTDALRFTLCSHGVQVGDLHLSVSEVQSCRHFCNKIWNALRFILNALGEKFVPQPAEELSPSSPMDAWILSRLALAAQECERGFLTRELSLVTHALHHFWLHNLCDVYLDPWPAPLAGSSGCREEGCGPGPVL

Target ORF information:

RefSeq Version	XM_016956719.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes valyl-tRNA synthetase 2, mitochondrial (VARS2), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016956719.2

ORF Insert Sequence:

ATGGGCGGGA AGGCCTGGCC GCGCCGGGCT GTGGGCACTG CAGGAGGCCC CTGTGCAGAA 
CAGATCTCGG CCCCTTTCCA AACACTCCTG ATGCCTCATT TGCCTCTCGC CTCTTTTCGA 
CCACCATTTT GGGGGCTGAG GCACTCACGG GGCCTCCCCA GGTTTCACTC CGTTTCTACA 
CAGTCGGAGC CCCATGGATC TCCCATCTCC CGGAGGAACC GTGAAGCCAA ACAGAAGCGC 
CTGCGAGAGA AGCAGGCGAC TCTGGAGGCT GAGATAGCAG GGGAGAGCAA GTCACCTGCA 
GAATCCATTA AGGCCTGGAG GCCTAAGGAG TTAGTATTGT ATGAAATCCC TACGAAACCC 
GGTGAAAAGA AAGATGTCTC TGGGCCCCTG CCTCCTGCAT ACAGCCCCCG ATATGTTGAG 
GCTGCCTGGT ACCCGTGGTG GGTACGAGAG GGCTTCTTCA AACCAGAATA TCAGGCCCGG 
CTGCCCCAAG CTACAGGGGA GACCTTTTCC ATGTGTATCC CACCTCCCAA TGTCACTGGC 
TCCCTGCACA TTGGCCACGC ACTCACGGTG GCCATACAGG ATGCCCTCGT GCGCTGGCAC 
CGGATGCGTG GGGATCAAGT GCTGTGGGTC CCTGGTTCAG ATCATGCAGG AATTGCTACA 
CAAGCTGTGG TGGAGAAACA ACTGTGGAAG GAACGGGGAG TGAGGAGACA TGAGCTGAGC 
CGGGAGGCCT TCCTTAGGGA GGTGTGGCAG TGGAAGGAGG CGAAAGGTGG AGAGATCTGT 
GAGCAGCTGC GAGCTCTGGG TGCCTCCCTG GACTGGGATC GAGAGTGTTT TACCATGGAT 
GTTGGCTCCT CAGTGGCTGT GACTGAAGCT TTTGTGCAGC TCTACAAGGC GGGGTTGCTG 
TACCGGAACC ATCAGCTTGT CAACTGGTCA TGTGCTTTAA GATCAGCCAT CTCAGACATT 
GAGGTGGAGA ACCGGCCCCT GCCTGGCCAC ACACAGCTTC GACTGCCTGG CTGCCCCACC 
CCCGTGTCTT TTGGCCTCCT ATTTTCTATT GCCTTCCCGG TGGATGGAGA GCCTGATGCA 
GAGGTTGTGG TAGGAACCAC AAGGCCAGAG ACGCTGCCTG GAGATGTGGC TGTGGCCGTT 
CATCCAGACG ACTCGCGATA CACACATCTA CACGGGCGAC AGCTTCGTCA CCCCTTGATG 
GGGCAGCCTC TTCCCCTCAT CACAGACTAT GCTGTTCAGC CACATGTGGG CACGGGGGCA 
GTGAAGGTGA CTCCAGCTCA CAGTCCTGCC GATGCTGAGA TGGGGGCCCG ACATGGCTTG 
AGCCCCTTGA ATGTCATTGC GGAGGATGGG ACCATGACCT CCCTCTGCGG GGACTGGCTG 
CAGGGTCTTC ACCGGTTTGT GGCCCGGGAA AAGATAATGT CTGTGCTGAG TGAACGGGGC 
CTGTTCCGGG GCCTCCAGAA CCACCCCATG GTACTGCCCA TCTGCAGCCG TTCTGGGGAT 
GTGATAGAAT ACCTGCTGAA GAACCAGTGG TTTGTCCGCT GCCAGGAAAT GGGGGCCCGA 
GCTGCCAAGG CTGTGGAGTC GGGGGCCCTG GAGCTCAGTC CCTCCTTCCA CCAGAAGAAT 
TGGCAGCACT GGTTTTCCCA TATTGGGGAC TGGTGTGTCT CCCGGCAGCT GTGGTGGGGC 
CATCAGATTC CAGCCTACCT GGTTGTAGAG GACCATGCGC AGGGAGAAGA GGACTGTTGG 
GTGGTTGGGC GGTCAGAGGC TGAGGCCAGA GAGGTAGCAG CGGAACTGAC AGGGAGGCCA 
GGGGCAGAGC TGACCCTGGA GAGGGATCCT GATGTCCTAG ACACATGGTT TTCTTCTGCC 
CTGTTCCCCT TTTCTGCCCT GGGCTGGCCC CAAGAGACCC CAGACCTTGC TCGTTTCTAC 
CCCCTGTCAC TTTTGGAAAC GGGCAGTGAC CTTCTGCTGT TCTGGGTGGG CCGCATGGTC 
ATGTTGGGGA CCCAGCTCAC AGGGCAGCTG CCCTTCAGCA AGGTGCTTCT TCATCCCATG 
GTTCGGGACA GGCAGGGCCG GAAGATGAGC AAGTCCCTGG GGAATGTGCT GGACCCAAGA 
GACATCATCA GTGGGGTGGA GATGCAGGTG CTGCAGGAAA AGCTGAGAAG CGGAAATTTG 
GACCCTGCAG AGCTGGCCAT TGTGGCTGCA GCACAGAAAA AGGACTTTCC TCACGGGATC 
CCTGAGTGTG GGACAGATGC CCTGAGATTC ACACTCTGCT CCCATGGAGT TCAGGTGGGC 
GACTTGCACC TGTCAGTCTC TGAGGTCCAG AGCTGCCGAC ATTTCTGCAA CAAGATCTGG 
AATGCTCTTC GCTTTATCCT CAATGCTTTA GGGGAGAAAT TTGTGCCACA GCCTGCTGAG 
GAGCTGTCTC CCTCCTCCCC GATGGATGCC TGGATCTTGA GCCGCCTTGC CCTGGCTGCC 
CAGGAGTGTG AGCGGGGCTT CCTCACCCGA GAGCTCTCGC TCGTCACTCA TGCCCTGCAC 
CACTTCTGGC TTCACAACCT CTGTGACGTC TACCTGGACC CATGGCCTGC CCCGCTGGCA 
GGTAGCAGTG GCTGTAGGGA GGAGGGCTGT GGCCCTGGAC CTGTCCTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VARS2 ( XM_016956719.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016812208.1 Pan troglodytes valyl-tRNA synthetase 2, mitochondrial (VARS2), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OPc87418
Clone ID Related Accession (Same CDS sequence)	XM_016956719.2 , XM_016956719.1
Accession Version	XM_016956719.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2631bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	valine--tRNA ligase, mitochondrial isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006473.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCGGGA AGGCCTGGCC GCGCCGGGCT GTGGGCACTG CAGGAGGCCC CTGTGCAGAA 
CAGATCTCGG CCCCTTTCCA AACACTCCTG ATGCCTCATT TGCCTCTCGC CTCTTTTCGA 
CCACCATTTT GGGGGCTGAG GCACTCACGG GGCCTCCCCA GGTTTCACTC CGTTTCTACA 
CAGTCGGAGC CCCATGGATC TCCCATCTCC CGGAGGAACC GTGAAGCCAA ACAGAAGCGC 
CTGCGAGAGA AGCAGGCGAC TCTGGAGGCT GAGATAGCAG GGGAGAGCAA GTCACCTGCA 
GAATCCATTA AGGCCTGGAG GCCTAAGGAG TTAGTATTGT ATGAAATCCC TACGAAACCC 
GGTGAAAAGA AAGATGTCTC TGGGCCCCTG CCTCCTGCAT ACAGCCCCCG ATATGTTGAG 
GCTGCCTGGT ACCCGTGGTG GGTACGAGAG GGCTTCTTCA AACCAGAATA TCAGGCCCGG 
CTGCCCCAAG CTACAGGGGA GACCTTTTCC ATGTGTATCC CACCTCCCAA TGTCACTGGC 
TCCCTGCACA TTGGCCACGC ACTCACGGTG GCCATACAGG ATGCCCTCGT GCGCTGGCAC 
CGGATGCGTG GGGATCAAGT GCTGTGGGTC CCTGGTTCAG ATCATGCAGG AATTGCTACA 
CAAGCTGTGG TGGAGAAACA ACTGTGGAAG GAACGGGGAG TGAGGAGACA TGAGCTGAGC 
CGGGAGGCCT TCCTTAGGGA GGTGTGGCAG TGGAAGGAGG CGAAAGGTGG AGAGATCTGT 
GAGCAGCTGC GAGCTCTGGG TGCCTCCCTG GACTGGGATC GAGAGTGTTT TACCATGGAT 
GTTGGCTCCT CAGTGGCTGT GACTGAAGCT TTTGTGCAGC TCTACAAGGC GGGGTTGCTG 
TACCGGAACC ATCAGCTTGT CAACTGGTCA TGTGCTTTAA GATCAGCCAT CTCAGACATT 
GAGGTGGAGA ACCGGCCCCT GCCTGGCCAC ACACAGCTTC GACTGCCTGG CTGCCCCACC 
CCCGTGTCTT TTGGCCTCCT ATTTTCTATT GCCTTCCCGG TGGATGGAGA GCCTGATGCA 
GAGGTTGTGG TAGGAACCAC AAGGCCAGAG ACGCTGCCTG GAGATGTGGC TGTGGCCGTT 
CATCCAGACG ACTCGCGATA CACACATCTA CACGGGCGAC AGCTTCGTCA CCCCTTGATG 
GGGCAGCCTC TTCCCCTCAT CACAGACTAT GCTGTTCAGC CACATGTGGG CACGGGGGCA 
GTGAAGGTGA CTCCAGCTCA CAGTCCTGCC GATGCTGAGA TGGGGGCCCG ACATGGCTTG 
AGCCCCTTGA ATGTCATTGC GGAGGATGGG ACCATGACCT CCCTCTGCGG GGACTGGCTG 
CAGGGTCTTC ACCGGTTTGT GGCCCGGGAA AAGATAATGT CTGTGCTGAG TGAACGGGGC 
CTGTTCCGGG GCCTCCAGAA CCACCCCATG GTACTGCCCA TCTGCAGCCG TTCTGGGGAT 
GTGATAGAAT ACCTGCTGAA GAACCAGTGG TTTGTCCGCT GCCAGGAAAT GGGGGCCCGA 
GCTGCCAAGG CTGTGGAGTC GGGGGCCCTG GAGCTCAGTC CCTCCTTCCA CCAGAAGAAT 
TGGCAGCACT GGTTTTCCCA TATTGGGGAC TGGTGTGTCT CCCGGCAGCT GTGGTGGGGC 
CATCAGATTC CAGCCTACCT GGTTGTAGAG GACCATGCGC AGGGAGAAGA GGACTGTTGG 
GTGGTTGGGC GGTCAGAGGC TGAGGCCAGA GAGGTAGCAG CGGAACTGAC AGGGAGGCCA 
GGGGCAGAGC TGACCCTGGA GAGGGATCCT GATGTCCTAG ACACATGGTT TTCTTCTGCC 
CTGTTCCCCT TTTCTGCCCT GGGCTGGCCC CAAGAGACCC CAGACCTTGC TCGTTTCTAC 
CCCCTGTCAC TTTTGGAAAC GGGCAGTGAC CTTCTGCTGT TCTGGGTGGG CCGCATGGTC 
ATGTTGGGGA CCCAGCTCAC AGGGCAGCTG CCCTTCAGCA AGGTGCTTCT TCATCCCATG 
GTTCGGGACA GGCAGGGCCG GAAGATGAGC AAGTCCCTGG GGAATGTGCT GGACCCAAGA 
GACATCATCA GTGGGGTGGA GATGCAGGTG CTGCAGGAAA AGCTGAGAAG CGGAAATTTG 
GACCCTGCAG AGCTGGCCAT TGTGGCTGCA GCACAGAAAA AGGACTTTCC TCACGGGATC 
CCTGAGTGTG GGACAGATGC CCTGAGATTC ACACTCTGCT CCCATGGAGT TCAGGTGGGC 
GACTTGCACC TGTCAGTCTC TGAGGTCCAG AGCTGCCGAC ATTTCTGCAA CAAGATCTGG 
AATGCTCTTC GCTTTATCCT CAATGCTTTA GGGGAGAAAT TTGTGCCACA GCCTGCTGAG 
GAGCTGTCTC CCTCCTCCCC GATGGATGCC TGGATCTTGA GCCGCCTTGC CCTGGCTGCC 
CAGGAGTGTG AGCGGGGCTT CCTCACCCGA GAGCTCTCGC TCGTCACTCA TGCCCTGCAC 
CACTTCTGGC TTCACAACCT CTGTGACGTC TACCTGGACC CATGGCCTGC CCCGCTGGCA 
GGTAGCAGTG GCTGTAGGGA GGAGGGCTGT GGCCCTGGAC CTGTCCTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016812208.1
CDS	82..2712
Translation	MGGKAWPRRAVGTAGGPCAEQISAPFQTLLMPHLPLASFRPPFWGLRHSRGLPRFHSVSTQSEPHGSPISRRNREAKQKRLREKQATLEAEIAGESKSPAESIKAWRPKELVLYEIPTKPGEKKDVSGPLPPAYSPRYVEAAWYPWWVREGFFKPEYQARLPQATGETFSMCIPPPNVTGSLHIGHALTVAIQDALVRWHRMRGDQVLWVPGSDHAGIATQAVVEKQLWKERGVRRHELSREAFLREVWQWKEAKGGEICEQLRALGASLDWDRECFTMDVGSSVAVTEAFVQLYKAGLLYRNHQLVNWSCALRSAISDIEVENRPLPGHTQLRLPGCPTPVSFGLLFSIAFPVDGEPDAEVVVGTTRPETLPGDVAVAVHPDDSRYTHLHGRQLRHPLMGQPLPLITDYAVQPHVGTGAVKVTPAHSPADAEMGARHGLSPLNVIAEDGTMTSLCGDWLQGLHRFVAREKIMSVLSERGLFRGLQNHPMVLPICSRSGDVIEYLLKNQWFVRCQEMGARAAKAVESGALELSPSFHQKNWQHWFSHIGDWCVSRQLWWGHQIPAYLVVEDHAQGEEDCWVVGRSEAEAREVAAELTGRPGAELTLERDPDVLDTWFSSALFPFSALGWPQETPDLARFYPLSLLETGSDLLLFWVGRMVMLGTQLTGQLPFSKVLLHPMVRDRQGRKMSKSLGNVLDPRDIISGVEMQVLQEKLRSGNLDPAELAIVAAAQKKDFPHGIPECGTDALRFTLCSHGVQVGDLHLSVSEVQSCRHFCNKIWNALRFILNALGEKFVPQPAEELSPSSPMDAWILSRLALAAQECERGFLTRELSLVTHALHHFWLHNLCDVYLDPWPAPLAGSSGCREEGCGPGPVL

Target ORF information:

RefSeq Version	XM_016956719.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes valyl-tRNA synthetase 2, mitochondrial (VARS2), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016956719.1

ORF Insert Sequence:

ATGGGCGGGA AGGCCTGGCC GCGCCGGGCT GTGGGCACTG CAGGAGGCCC CTGTGCAGAA 
CAGATCTCGG CCCCTTTCCA AACACTCCTG ATGCCTCATT TGCCTCTCGC CTCTTTTCGA 
CCACCATTTT GGGGGCTGAG GCACTCACGG GGCCTCCCCA GGTTTCACTC CGTTTCTACA 
CAGTCGGAGC CCCATGGATC TCCCATCTCC CGGAGGAACC GTGAAGCCAA ACAGAAGCGC 
CTGCGAGAGA AGCAGGCGAC TCTGGAGGCT GAGATAGCAG GGGAGAGCAA GTCACCTGCA 
GAATCCATTA AGGCCTGGAG GCCTAAGGAG TTAGTATTGT ATGAAATCCC TACGAAACCC 
GGTGAAAAGA AAGATGTCTC TGGGCCCCTG CCTCCTGCAT ACAGCCCCCG ATATGTTGAG 
GCTGCCTGGT ACCCGTGGTG GGTACGAGAG GGCTTCTTCA AACCAGAATA TCAGGCCCGG 
CTGCCCCAAG CTACAGGGGA GACCTTTTCC ATGTGTATCC CACCTCCCAA TGTCACTGGC 
TCCCTGCACA TTGGCCACGC ACTCACGGTG GCCATACAGG ATGCCCTCGT GCGCTGGCAC 
CGGATGCGTG GGGATCAAGT GCTGTGGGTC CCTGGTTCAG ATCATGCAGG AATTGCTACA 
CAAGCTGTGG TGGAGAAACA ACTGTGGAAG GAACGGGGAG TGAGGAGACA TGAGCTGAGC 
CGGGAGGCCT TCCTTAGGGA GGTGTGGCAG TGGAAGGAGG CGAAAGGTGG AGAGATCTGT 
GAGCAGCTGC GAGCTCTGGG TGCCTCCCTG GACTGGGATC GAGAGTGTTT TACCATGGAT 
GTTGGCTCCT CAGTGGCTGT GACTGAAGCT TTTGTGCAGC TCTACAAGGC GGGGTTGCTG 
TACCGGAACC ATCAGCTTGT CAACTGGTCA TGTGCTTTAA GATCAGCCAT CTCAGACATT 
GAGGTGGAGA ACCGGCCCCT GCCTGGCCAC ACACAGCTTC GACTGCCTGG CTGCCCCACC 
CCCGTGTCTT TTGGCCTCCT ATTTTCTATT GCCTTCCCGG TGGATGGAGA GCCTGATGCA 
GAGGTTGTGG TAGGAACCAC AAGGCCAGAG ACGCTGCCTG GAGATGTGGC TGTGGCCGTT 
CATCCAGACG ACTCGCGATA CACACATCTA CACGGGCGAC AGCTTCGTCA CCCCTTGATG 
GGGCAGCCTC TTCCCCTCAT CACAGACTAT GCTGTTCAGC CACATGTGGG CACGGGGGCA 
GTGAAGGTGA CTCCAGCTCA CAGTCCTGCC GATGCTGAGA TGGGGGCCCG ACATGGCTTG 
AGCCCCTTGA ATGTCATTGC GGAGGATGGG ACCATGACCT CCCTCTGCGG GGACTGGCTG 
CAGGGTCTTC ACCGGTTTGT GGCCCGGGAA AAGATAATGT CTGTGCTGAG TGAACGGGGC 
CTGTTCCGGG GCCTCCAGAA CCACCCCATG GTACTGCCCA TCTGCAGCCG TTCTGGGGAT 
GTGATAGAAT ACCTGCTGAA GAACCAGTGG TTTGTCCGCT GCCAGGAAAT GGGGGCCCGA 
GCTGCCAAGG CTGTGGAGTC GGGGGCCCTG GAGCTCAGTC CCTCCTTCCA CCAGAAGAAT 
TGGCAGCACT GGTTTTCCCA TATTGGGGAC TGGTGTGTCT CCCGGCAGCT GTGGTGGGGC 
CATCAGATTC CAGCCTACCT GGTTGTAGAG GACCATGCGC AGGGAGAAGA GGACTGTTGG 
GTGGTTGGGC GGTCAGAGGC TGAGGCCAGA GAGGTAGCAG CGGAACTGAC AGGGAGGCCA 
GGGGCAGAGC TGACCCTGGA GAGGGATCCT GATGTCCTAG ACACATGGTT TTCTTCTGCC 
CTGTTCCCCT TTTCTGCCCT GGGCTGGCCC CAAGAGACCC CAGACCTTGC TCGTTTCTAC 
CCCCTGTCAC TTTTGGAAAC GGGCAGTGAC CTTCTGCTGT TCTGGGTGGG CCGCATGGTC 
ATGTTGGGGA CCCAGCTCAC AGGGCAGCTG CCCTTCAGCA AGGTGCTTCT TCATCCCATG 
GTTCGGGACA GGCAGGGCCG GAAGATGAGC AAGTCCCTGG GGAATGTGCT GGACCCAAGA 
GACATCATCA GTGGGGTGGA GATGCAGGTG CTGCAGGAAA AGCTGAGAAG CGGAAATTTG 
GACCCTGCAG AGCTGGCCAT TGTGGCTGCA GCACAGAAAA AGGACTTTCC TCACGGGATC 
CCTGAGTGTG GGACAGATGC CCTGAGATTC ACACTCTGCT CCCATGGAGT TCAGGTGGGC 
GACTTGCACC TGTCAGTCTC TGAGGTCCAG AGCTGCCGAC ATTTCTGCAA CAAGATCTGG 
AATGCTCTTC GCTTTATCCT CAATGCTTTA GGGGAGAAAT TTGTGCCACA GCCTGCTGAG 
GAGCTGTCTC CCTCCTCCCC GATGGATGCC TGGATCTTGA GCCGCCTTGC CCTGGCTGCC 
CAGGAGTGTG AGCGGGGCTT CCTCACCCGA GAGCTCTCGC TCGTCACTCA TGCCCTGCAC 
CACTTCTGGC TTCACAACCT CTGTGACGTC TACCTGGACC CATGGCCTGC CCCGCTGGCA 
GGTAGCAGTG GCTGTAGGGA GGAGGGCTGT GGCCCTGGAC CTGTCCTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VARS2 ( XM_016956713.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016812202.1 Pan troglodytes valyl-tRNA synthetase 2, mitochondrial (VARS2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OPc87416
Clone ID Related Accession (Same CDS sequence)	XM_016956713.1
Accession Version	XM_016956713.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3282bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	valine--tRNA ligase, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036885.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCGGGA AGGCCTGGCC GCGCCGGGCT GTGGGCACTG CAGGAGGCCC CTGTGCAGAA 
CAGATCTCGG CCCCTTTCCA AACACTCCTG ATGCCTCATT TGCCTCTCGC CTCTTTTCGA 
CCACCATTTT GGGGGCTGAG GCACTCACGG GGCCTCCCCA GGTTTCACTC CGTTTCTACA 
CAGTCGGAGC CCCATGGATC TCCCATCTCC CGGAGGAACC GTGAAGCCAA ACAGAAGCGC 
CTGCGAGAGA AGCAGGCGAC TCTGGAGGCT GAGATAGCAG GGGAGAGCAA GTCACCTGCA 
GAATCCATTA AGGCCTGGAG GCCTAAGGAG TTAGTATTGT ATGAAATCCC TACGAAACCC 
GGTGAAAAGA AAGATGTCTC TGGGCCCCTG CCTCCTGCAT ACAGCCCCCG ATATGTTGAG 
GCTGCCTGGT ACCCGTGGTG GGTACGAGAG GGCTTCTTCA AACCAGAATA TCAGGCCCGG 
CTGCCCCAAG CTACAGGGGA GACCTTTTCC ATGTGTATCC CACCTCCCAA TGTCACTGGC 
TCCCTGCACA TTGGCCACGC ACTCACGGTG GCCATACAGG ATGCCCTCGT GCGCTGGCAC 
CGGATGCGTG GGGATCAAGT GCTGTGGGTC CCTGGTTCAG ATCATGCAGG AATTGCTACA 
CAAGCTGTGG TGGAGAAACA ACTGTGGAAG GAACGGGGAG TGAGGAGACA TGAGCTGAGC 
CGGGAGGCCT TCCTTAGGGA GGTGTGGCAG TGGAAGGAGG CGAAAGGTGG AGAGATCTGT 
GAGCAGCTGC GAGCTCTGGG TGCCTCCCTG GACTGGGATC GAGAGTGTTT TACCATGGAT 
GTTGGCTCCT CAGTGGCTGT GACTGAAGCT TTTGTGCAGC TCTACAAGGC GGGGTTGCTG 
TACCGGAACC ATCAGCTTGT CAACTGGTCA TGTGCTTTAA GATCAGCCAT CTCAGACATT 
GAGGTGGAGA ACCGGCCCCT GCCTGGCCAC ACACAGCTTC GACTGCCTGG CTGCCCCACC 
CCCGTGTCTT TTGGCCTCCT ATTTTCTATT GCCTTCCCGG TGGATGGAGA GCCTGATGCA 
GAGGTTGTGG TAGGAACCAC AAGGCCAGAG ACGCTGCCTG GAGATGTGGC TGTGGCCGTT 
CATCCAGACG ACTCGCGATA CACACATCTA CACGGGCGAC AGCTTCGTCA CCCCTTGATG 
GGGCAGCCTC TTCCCCTCAT CACAGACTAT GCTGTTCAGC CACATGTGGG CACGGGGGCA 
GTGAAGGTGA CTCCAGCTCA CAGTCCTGCC GATGCTGAGA TGGGGGCCCG ACATGGCTTG 
AGCCCCTTGA ATGTCATTGC GGAGGATGGG ACCATGACCT CCCTCTGCGG GGACTGGCTG 
CAGGGTCTTC ACCGGTTTGT GGCCCGGGAA AAGATAATGT CTGTGCTGAG TGAACGGGGC 
CTGTTCCGGG GCCTCCAGAA CCACCCCATG GTACTGCCCA TCTGCAGCCG TTCTGGGGAT 
GTGATAGAAT ACCTGCTGAA GAACCAGTGG TTTGTCCGCT GCCAGGAAAT GGGGGCCCGA 
GCTGCCAAGG CTGTGGAGTC GGGGGCCCTG GAGCTCAGTC CCTCCTTCCA CCAGAAGAAT 
TGGCAGCACT GGTTTTCCCA TATTGGGGAC TGGTGTGTCT CCCGGCAGCT GTGGTGGGGC 
CATCAGATTC CAGCCTACCT GGTTGTAGAG GACCATGCGC AGGGAGAAGA GGACTGTTGG 
GTGGTTGGGC GGTCAGAGGC TGAGGCCAGA GAGGTAGCAG CGGAACTGAC AGGGAGGCCA 
GGGGCAGAGC TGACCCTGGA GAGGGATCCT GATGTCCTAG ACACATGGTT TTCTTCTGCC 
CTGTTCCCCT TTTCTGCCCT GGGCTGGCCC CAAGAGACCC CAGACCTTGC TCGTTTCTAC 
CCCCTGTCAC TTTTGGAAAC GGGCAGTGAC CTTCTGCTGT TCTGGGTGGG CCGCATGGTC 
ATGTTGGGGA CCCAGCTCAC AGGGCAGCTG CCCTTCAGCA AGGTGCTTCT TCATCCCATG 
GTTCGGGACA GGCAGGGCCG GAAGATGAGC AAGTCCCTGG GGAATGTGCT GGACCCAAGA 
GACATCATCA GTGGGGTGGA GATGCAGGTG CTGCAGGAAA AGCTGAGAAG CGGAAATTTG 
GACCCTGCAG AGCTGGCCAT TGTGGCTGCA GCACAGAAAA AGGACTTTCC TCACGGGATC 
CCTGAGTGTG GGACAGATGC CCTGAGATTC ACACTCTGCT CCCATGGAGT TCAGGTGGGC 
GACTTGCACC TGTCAGTCTC TGAGGTCCAG AGCTGCCGAC ATTTCTGCAA CAAGATCTGG 
AATGCTCTTC GCTTTATCCT CAATGCTTTA GGGGAGAAAT TTGTGCCACA GCCTGCTGAG 
GAGCTGTCTC CCTCCTCCCC GATGGATGCC TGGATCTTGA GCCGCCTTGC CCTGGCTGCC 
CAGGAGTGTG AGCGGGGCTT CCTCACCCGA GAGCTCTCGC TCGTCACTCA TGCCCTGCAC 
CACTTCTGGC TTCACAACCT CTGTGACGTC TACCTGGAGG CTGTGAAGCC CGTGCTGTGG 
CACTCGCCCC GCCCCCTGGG GCCCCCTCAG GTCCTGTTCT CCTGCGCTGA CCTCGGCCTC 
CGCCTCCTGG CCCCACTGAT GCCCTTCCTG GCTGAAGAGC TCTGGCAGAG GCTGCCCCCC 
AGGGCTGGTT GCCCCCCTGC CCCCAGCATC TCGGTTGCCC CCTACCCCAG CGCCTGCAGC 
TTGGAGCACT GGCGCCAGCC AGAGCTGGAG CGGCGCTTCT CCCGGGTCCA AGAGGTCGTG 
CAGGTGCTAA GGGCTCTCCG AGCCACGTAC CAGCTCACCA AAGCCCGGCC CCGAGTGCTG 
CTGCAGAGCT CAGAGCCTGG GGACCAGGGC CTCTTCGAGG CCTTCTTGGA GCCCCTGGGC 
ACCCTGGGCT ACTGTGGGGC TGTGGGCCTG TTACCCCCAG GCGCAGCAGC TCCCTCCGGC 
TGGGCCCAGG CTCCACTCAG TGACACGGCT CAAGTCTACA TGGAGCTGCA GGGCCTGGTG 
GACCCGCAGA TCCAGCTACC TCTGTTAGCC GCCCGAAGGT ACAAGTTGCA GAAGCAGCTT 
GATAGCCTCA CAGCCAGGAC CCCATCAGAA GGGGAGGCAG GGACTCAGAG GCAACAAAAG 
CTTTCTTCCC TCCAGCTGGA ATTGTCAAAA CTGGACAAGG CAGCCTCTCA CCTCCGGCAG 
CTGATGGATG AGCCTCCAGC CCCAGGGAGC CCGGAGCTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016812202.1
CDS	82..3363
Translation	MGGKAWPRRAVGTAGGPCAEQISAPFQTLLMPHLPLASFRPPFWGLRHSRGLPRFHSVSTQSEPHGSPISRRNREAKQKRLREKQATLEAEIAGESKSPAESIKAWRPKELVLYEIPTKPGEKKDVSGPLPPAYSPRYVEAAWYPWWVREGFFKPEYQARLPQATGETFSMCIPPPNVTGSLHIGHALTVAIQDALVRWHRMRGDQVLWVPGSDHAGIATQAVVEKQLWKERGVRRHELSREAFLREVWQWKEAKGGEICEQLRALGASLDWDRECFTMDVGSSVAVTEAFVQLYKAGLLYRNHQLVNWSCALRSAISDIEVENRPLPGHTQLRLPGCPTPVSFGLLFSIAFPVDGEPDAEVVVGTTRPETLPGDVAVAVHPDDSRYTHLHGRQLRHPLMGQPLPLITDYAVQPHVGTGAVKVTPAHSPADAEMGARHGLSPLNVIAEDGTMTSLCGDWLQGLHRFVAREKIMSVLSERGLFRGLQNHPMVLPICSRSGDVIEYLLKNQWFVRCQEMGARAAKAVESGALELSPSFHQKNWQHWFSHIGDWCVSRQLWWGHQIPAYLVVEDHAQGEEDCWVVGRSEAEAREVAAELTGRPGAELTLERDPDVLDTWFSSALFPFSALGWPQETPDLARFYPLSLLETGSDLLLFWVGRMVMLGTQLTGQLPFSKVLLHPMVRDRQGRKMSKSLGNVLDPRDIISGVEMQVLQEKLRSGNLDPAELAIVAAAQKKDFPHGIPECGTDALRFTLCSHGVQVGDLHLSVSEVQSCRHFCNKIWNALRFILNALGEKFVPQPAEELSPSSPMDAWILSRLALAAQECERGFLTRELSLVTHALHHFWLHNLCDVYLEAVKPVLWHSPRPLGPPQVLFSCADLGLRLLAPLMPFLAEELWQRLPPRAGCPPAPSISVAPYPSACSLEHWRQPELERRFSRVQEVVQVLRALRATYQLTKARPRVLLQSSEPGDQGLFEAFLEPLGTLGYCGAVGLLPPGAAAPSGWAQAPLSDTAQVYMELQGLVDPQIQLPLLAARRYKLQKQLDSLTARTPSEGEAGTQRQQKLSSLQLELSKLDKAASHLRQLMDEPPAPGSPEL

Target ORF information:

RefSeq Version	XM_016956713.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes valyl-tRNA synthetase 2, mitochondrial (VARS2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016956713.1

ORF Insert Sequence:

ATGGGCGGGA AGGCCTGGCC GCGCCGGGCT GTGGGCACTG CAGGAGGCCC CTGTGCAGAA 
CAGATCTCGG CCCCTTTCCA AACACTCCTG ATGCCTCATT TGCCTCTCGC CTCTTTTCGA 
CCACCATTTT GGGGGCTGAG GCACTCACGG GGCCTCCCCA GGTTTCACTC CGTTTCTACA 
CAGTCGGAGC CCCATGGATC TCCCATCTCC CGGAGGAACC GTGAAGCCAA ACAGAAGCGC 
CTGCGAGAGA AGCAGGCGAC TCTGGAGGCT GAGATAGCAG GGGAGAGCAA GTCACCTGCA 
GAATCCATTA AGGCCTGGAG GCCTAAGGAG TTAGTATTGT ATGAAATCCC TACGAAACCC 
GGTGAAAAGA AAGATGTCTC TGGGCCCCTG CCTCCTGCAT ACAGCCCCCG ATATGTTGAG 
GCTGCCTGGT ACCCGTGGTG GGTACGAGAG GGCTTCTTCA AACCAGAATA TCAGGCCCGG 
CTGCCCCAAG CTACAGGGGA GACCTTTTCC ATGTGTATCC CACCTCCCAA TGTCACTGGC 
TCCCTGCACA TTGGCCACGC ACTCACGGTG GCCATACAGG ATGCCCTCGT GCGCTGGCAC 
CGGATGCGTG GGGATCAAGT GCTGTGGGTC CCTGGTTCAG ATCATGCAGG AATTGCTACA 
CAAGCTGTGG TGGAGAAACA ACTGTGGAAG GAACGGGGAG TGAGGAGACA TGAGCTGAGC 
CGGGAGGCCT TCCTTAGGGA GGTGTGGCAG TGGAAGGAGG CGAAAGGTGG AGAGATCTGT 
GAGCAGCTGC GAGCTCTGGG TGCCTCCCTG GACTGGGATC GAGAGTGTTT TACCATGGAT 
GTTGGCTCCT CAGTGGCTGT GACTGAAGCT TTTGTGCAGC TCTACAAGGC GGGGTTGCTG 
TACCGGAACC ATCAGCTTGT CAACTGGTCA TGTGCTTTAA GATCAGCCAT CTCAGACATT 
GAGGTGGAGA ACCGGCCCCT GCCTGGCCAC ACACAGCTTC GACTGCCTGG CTGCCCCACC 
CCCGTGTCTT TTGGCCTCCT ATTTTCTATT GCCTTCCCGG TGGATGGAGA GCCTGATGCA 
GAGGTTGTGG TAGGAACCAC AAGGCCAGAG ACGCTGCCTG GAGATGTGGC TGTGGCCGTT 
CATCCAGACG ACTCGCGATA CACACATCTA CACGGGCGAC AGCTTCGTCA CCCCTTGATG 
GGGCAGCCTC TTCCCCTCAT CACAGACTAT GCTGTTCAGC CACATGTGGG CACGGGGGCA 
GTGAAGGTGA CTCCAGCTCA CAGTCCTGCC GATGCTGAGA TGGGGGCCCG ACATGGCTTG 
AGCCCCTTGA ATGTCATTGC GGAGGATGGG ACCATGACCT CCCTCTGCGG GGACTGGCTG 
CAGGGTCTTC ACCGGTTTGT GGCCCGGGAA AAGATAATGT CTGTGCTGAG TGAACGGGGC 
CTGTTCCGGG GCCTCCAGAA CCACCCCATG GTACTGCCCA TCTGCAGCCG TTCTGGGGAT 
GTGATAGAAT ACCTGCTGAA GAACCAGTGG TTTGTCCGCT GCCAGGAAAT GGGGGCCCGA 
GCTGCCAAGG CTGTGGAGTC GGGGGCCCTG GAGCTCAGTC CCTCCTTCCA CCAGAAGAAT 
TGGCAGCACT GGTTTTCCCA TATTGGGGAC TGGTGTGTCT CCCGGCAGCT GTGGTGGGGC 
CATCAGATTC CAGCCTACCT GGTTGTAGAG GACCATGCGC AGGGAGAAGA GGACTGTTGG 
GTGGTTGGGC GGTCAGAGGC TGAGGCCAGA GAGGTAGCAG CGGAACTGAC AGGGAGGCCA 
GGGGCAGAGC TGACCCTGGA GAGGGATCCT GATGTCCTAG ACACATGGTT TTCTTCTGCC 
CTGTTCCCCT TTTCTGCCCT GGGCTGGCCC CAAGAGACCC CAGACCTTGC TCGTTTCTAC 
CCCCTGTCAC TTTTGGAAAC GGGCAGTGAC CTTCTGCTGT TCTGGGTGGG CCGCATGGTC 
ATGTTGGGGA CCCAGCTCAC AGGGCAGCTG CCCTTCAGCA AGGTGCTTCT TCATCCCATG 
GTTCGGGACA GGCAGGGCCG GAAGATGAGC AAGTCCCTGG GGAATGTGCT GGACCCAAGA 
GACATCATCA GTGGGGTGGA GATGCAGGTG CTGCAGGAAA AGCTGAGAAG CGGAAATTTG 
GACCCTGCAG AGCTGGCCAT TGTGGCTGCA GCACAGAAAA AGGACTTTCC TCACGGGATC 
CCTGAGTGTG GGACAGATGC CCTGAGATTC ACACTCTGCT CCCATGGAGT TCAGGTGGGC 
GACTTGCACC TGTCAGTCTC TGAGGTCCAG AGCTGCCGAC ATTTCTGCAA CAAGATCTGG 
AATGCTCTTC GCTTTATCCT CAATGCTTTA GGGGAGAAAT TTGTGCCACA GCCTGCTGAG 
GAGCTGTCTC CCTCCTCCCC GATGGATGCC TGGATCTTGA GCCGCCTTGC CCTGGCTGCC 
CAGGAGTGTG AGCGGGGCTT CCTCACCCGA GAGCTCTCGC TCGTCACTCA TGCCCTGCAC 
CACTTCTGGC TTCACAACCT CTGTGACGTC TACCTGGAGG CTGTGAAGCC CGTGCTGTGG 
CACTCGCCCC GCCCCCTGGG GCCCCCTCAG GTCCTGTTCT CCTGCGCTGA CCTCGGCCTC 
CGCCTCCTGG CCCCACTGAT GCCCTTCCTG GCTGAAGAGC TCTGGCAGAG GCTGCCCCCC 
AGGGCTGGTT GCCCCCCTGC CCCCAGCATC TCGGTTGCCC CCTACCCCAG CGCCTGCAGC 
TTGGAGCACT GGCGCCAGCC AGAGCTGGAG CGGCGCTTCT CCCGGGTCCA AGAGGTCGTG 
CAGGTGCTAA GGGCTCTCCG AGCCACGTAC CAGCTCACCA AAGCCCGGCC CCGAGTGCTG 
CTGCAGAGCT CAGAGCCTGG GGACCAGGGC CTCTTCGAGG CCTTCTTGGA GCCCCTGGGC 
ACCCTGGGCT ACTGTGGGGC TGTGGGCCTG TTACCCCCAG GCGCAGCAGC TCCCTCCGGC 
TGGGCCCAGG CTCCACTCAG TGACACGGCT CAAGTCTACA TGGAGCTGCA GGGCCTGGTG 
GACCCGCAGA TCCAGCTACC TCTGTTAGCC GCCCGAAGGT ACAAGTTGCA GAAGCAGCTT 
GATAGCCTCA CAGCCAGGAC CCCATCAGAA GGGGAGGCAG GGACTCAGAG GCAACAAAAG 
CTTTCTTCCC TCCAGCTGGA ATTGTCAAAA CTGGACAAGG CAGCCTCTCA CCTCCGGCAG 
CTGATGGATG AGCCTCCAGC CCCAGGGAGC CCGGAGCTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VARS2 ( XM_016956717.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016812206.1 Pan troglodytes valyl-tRNA synthetase 2, mitochondrial (VARS2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OPc87417
Clone ID Related Accession (Same CDS sequence)	XM_016956717.1 , XM_016956716.2 , XM_016956716.1 , XM_016956718.1
Accession Version	XM_016956717.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3192bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	valine--tRNA ligase, mitochondrial isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036885.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTCATT TGCCTCTCGC CTCTTTTCGA CCACCATTTT GGGGGCTGAG GCACTCACGG 
GGCCTCCCCA GGTTTCACTC CGTTTCTACA CAGTCGGAGC CCCATGGATC TCCCATCTCC 
CGGAGGAACC GTGAAGCCAA ACAGAAGCGC CTGCGAGAGA AGCAGGCGAC TCTGGAGGCT 
GAGATAGCAG GGGAGAGCAA GTCACCTGCA GAATCCATTA AGGCCTGGAG GCCTAAGGAG 
TTAGTATTGT ATGAAATCCC TACGAAACCC GGTGAAAAGA AAGATGTCTC TGGGCCCCTG 
CCTCCTGCAT ACAGCCCCCG ATATGTTGAG GCTGCCTGGT ACCCGTGGTG GGTACGAGAG 
GGCTTCTTCA AACCAGAATA TCAGGCCCGG CTGCCCCAAG CTACAGGGGA GACCTTTTCC 
ATGTGTATCC CACCTCCCAA TGTCACTGGC TCCCTGCACA TTGGCCACGC ACTCACGGTG 
GCCATACAGG ATGCCCTCGT GCGCTGGCAC CGGATGCGTG GGGATCAAGT GCTGTGGGTC 
CCTGGTTCAG ATCATGCAGG AATTGCTACA CAAGCTGTGG TGGAGAAACA ACTGTGGAAG 
GAACGGGGAG TGAGGAGACA TGAGCTGAGC CGGGAGGCCT TCCTTAGGGA GGTGTGGCAG 
TGGAAGGAGG CGAAAGGTGG AGAGATCTGT GAGCAGCTGC GAGCTCTGGG TGCCTCCCTG 
GACTGGGATC GAGAGTGTTT TACCATGGAT GTTGGCTCCT CAGTGGCTGT GACTGAAGCT 
TTTGTGCAGC TCTACAAGGC GGGGTTGCTG TACCGGAACC ATCAGCTTGT CAACTGGTCA 
TGTGCTTTAA GATCAGCCAT CTCAGACATT GAGGTGGAGA ACCGGCCCCT GCCTGGCCAC 
ACACAGCTTC GACTGCCTGG CTGCCCCACC CCCGTGTCTT TTGGCCTCCT ATTTTCTATT 
GCCTTCCCGG TGGATGGAGA GCCTGATGCA GAGGTTGTGG TAGGAACCAC AAGGCCAGAG 
ACGCTGCCTG GAGATGTGGC TGTGGCCGTT CATCCAGACG ACTCGCGATA CACACATCTA 
CACGGGCGAC AGCTTCGTCA CCCCTTGATG GGGCAGCCTC TTCCCCTCAT CACAGACTAT 
GCTGTTCAGC CACATGTGGG CACGGGGGCA GTGAAGGTGA CTCCAGCTCA CAGTCCTGCC 
GATGCTGAGA TGGGGGCCCG ACATGGCTTG AGCCCCTTGA ATGTCATTGC GGAGGATGGG 
ACCATGACCT CCCTCTGCGG GGACTGGCTG CAGGGTCTTC ACCGGTTTGT GGCCCGGGAA 
AAGATAATGT CTGTGCTGAG TGAACGGGGC CTGTTCCGGG GCCTCCAGAA CCACCCCATG 
GTACTGCCCA TCTGCAGCCG TTCTGGGGAT GTGATAGAAT ACCTGCTGAA GAACCAGTGG 
TTTGTCCGCT GCCAGGAAAT GGGGGCCCGA GCTGCCAAGG CTGTGGAGTC GGGGGCCCTG 
GAGCTCAGTC CCTCCTTCCA CCAGAAGAAT TGGCAGCACT GGTTTTCCCA TATTGGGGAC 
TGGTGTGTCT CCCGGCAGCT GTGGTGGGGC CATCAGATTC CAGCCTACCT GGTTGTAGAG 
GACCATGCGC AGGGAGAAGA GGACTGTTGG GTGGTTGGGC GGTCAGAGGC TGAGGCCAGA 
GAGGTAGCAG CGGAACTGAC AGGGAGGCCA GGGGCAGAGC TGACCCTGGA GAGGGATCCT 
GATGTCCTAG ACACATGGTT TTCTTCTGCC CTGTTCCCCT TTTCTGCCCT GGGCTGGCCC 
CAAGAGACCC CAGACCTTGC TCGTTTCTAC CCCCTGTCAC TTTTGGAAAC GGGCAGTGAC 
CTTCTGCTGT TCTGGGTGGG CCGCATGGTC ATGTTGGGGA CCCAGCTCAC AGGGCAGCTG 
CCCTTCAGCA AGGTGCTTCT TCATCCCATG GTTCGGGACA GGCAGGGCCG GAAGATGAGC 
AAGTCCCTGG GGAATGTGCT GGACCCAAGA GACATCATCA GTGGGGTGGA GATGCAGGTG 
CTGCAGGAAA AGCTGAGAAG CGGAAATTTG GACCCTGCAG AGCTGGCCAT TGTGGCTGCA 
GCACAGAAAA AGGACTTTCC TCACGGGATC CCTGAGTGTG GGACAGATGC CCTGAGATTC 
ACACTCTGCT CCCATGGAGT TCAGGTGGGC GACTTGCACC TGTCAGTCTC TGAGGTCCAG 
AGCTGCCGAC ATTTCTGCAA CAAGATCTGG AATGCTCTTC GCTTTATCCT CAATGCTTTA 
GGGGAGAAAT TTGTGCCACA GCCTGCTGAG GAGCTGTCTC CCTCCTCCCC GATGGATGCC 
TGGATCTTGA GCCGCCTTGC CCTGGCTGCC CAGGAGTGTG AGCGGGGCTT CCTCACCCGA 
GAGCTCTCGC TCGTCACTCA TGCCCTGCAC CACTTCTGGC TTCACAACCT CTGTGACGTC 
TACCTGGAGG CTGTGAAGCC CGTGCTGTGG CACTCGCCCC GCCCCCTGGG GCCCCCTCAG 
GTCCTGTTCT CCTGCGCTGA CCTCGGCCTC CGCCTCCTGG CCCCACTGAT GCCCTTCCTG 
GCTGAAGAGC TCTGGCAGAG GCTGCCCCCC AGGGCTGGTT GCCCCCCTGC CCCCAGCATC 
TCGGTTGCCC CCTACCCCAG CGCCTGCAGC TTGGAGCACT GGCGCCAGCC AGAGCTGGAG 
CGGCGCTTCT CCCGGGTCCA AGAGGTCGTG CAGGTGCTAA GGGCTCTCCG AGCCACGTAC 
CAGCTCACCA AAGCCCGGCC CCGAGTGCTG CTGCAGAGCT CAGAGCCTGG GGACCAGGGC 
CTCTTCGAGG CCTTCTTGGA GCCCCTGGGC ACCCTGGGCT ACTGTGGGGC TGTGGGCCTG 
TTACCCCCAG GCGCAGCAGC TCCCTCCGGC TGGGCCCAGG CTCCACTCAG TGACACGGCT 
CAAGTCTACA TGGAGCTGCA GGGCCTGGTG GACCCGCAGA TCCAGCTACC TCTGTTAGCC 
GCCCGAAGGT ACAAGTTGCA GAAGCAGCTT GATAGCCTCA CAGCCAGGAC CCCATCAGAA 
GGGGAGGCAG GGACTCAGAG GCAACAAAAG CTTTCTTCCC TCCAGCTGGA ATTGTCAAAA 
CTGGACAAGG CAGCCTCTCA CCTCCGGCAG CTGATGGATG AGCCTCCAGC CCCAGGGAGC 
CCGGAGCTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016812206.1
CDS	184..3375
Translation	MPHLPLASFRPPFWGLRHSRGLPRFHSVSTQSEPHGSPISRRNREAKQKRLREKQATLEAEIAGESKSPAESIKAWRPKELVLYEIPTKPGEKKDVSGPLPPAYSPRYVEAAWYPWWVREGFFKPEYQARLPQATGETFSMCIPPPNVTGSLHIGHALTVAIQDALVRWHRMRGDQVLWVPGSDHAGIATQAVVEKQLWKERGVRRHELSREAFLREVWQWKEAKGGEICEQLRALGASLDWDRECFTMDVGSSVAVTEAFVQLYKAGLLYRNHQLVNWSCALRSAISDIEVENRPLPGHTQLRLPGCPTPVSFGLLFSIAFPVDGEPDAEVVVGTTRPETLPGDVAVAVHPDDSRYTHLHGRQLRHPLMGQPLPLITDYAVQPHVGTGAVKVTPAHSPADAEMGARHGLSPLNVIAEDGTMTSLCGDWLQGLHRFVAREKIMSVLSERGLFRGLQNHPMVLPICSRSGDVIEYLLKNQWFVRCQEMGARAAKAVESGALELSPSFHQKNWQHWFSHIGDWCVSRQLWWGHQIPAYLVVEDHAQGEEDCWVVGRSEAEAREVAAELTGRPGAELTLERDPDVLDTWFSSALFPFSALGWPQETPDLARFYPLSLLETGSDLLLFWVGRMVMLGTQLTGQLPFSKVLLHPMVRDRQGRKMSKSLGNVLDPRDIISGVEMQVLQEKLRSGNLDPAELAIVAAAQKKDFPHGIPECGTDALRFTLCSHGVQVGDLHLSVSEVQSCRHFCNKIWNALRFILNALGEKFVPQPAEELSPSSPMDAWILSRLALAAQECERGFLTRELSLVTHALHHFWLHNLCDVYLEAVKPVLWHSPRPLGPPQVLFSCADLGLRLLAPLMPFLAEELWQRLPPRAGCPPAPSISVAPYPSACSLEHWRQPELERRFSRVQEVVQVLRALRATYQLTKARPRVLLQSSEPGDQGLFEAFLEPLGTLGYCGAVGLLPPGAAAPSGWAQAPLSDTAQVYMELQGLVDPQIQLPLLAARRYKLQKQLDSLTARTPSEGEAGTQRQQKLSSLQLELSKLDKAASHLRQLMDEPPAPGSPEL

Target ORF information:

RefSeq Version	XM_016956717.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes valyl-tRNA synthetase 2, mitochondrial (VARS2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016956717.1

ORF Insert Sequence:

ATGCCTCATT TGCCTCTCGC CTCTTTTCGA CCACCATTTT GGGGGCTGAG GCACTCACGG 
GGCCTCCCCA GGTTTCACTC CGTTTCTACA CAGTCGGAGC CCCATGGATC TCCCATCTCC 
CGGAGGAACC GTGAAGCCAA ACAGAAGCGC CTGCGAGAGA AGCAGGCGAC TCTGGAGGCT 
GAGATAGCAG GGGAGAGCAA GTCACCTGCA GAATCCATTA AGGCCTGGAG GCCTAAGGAG 
TTAGTATTGT ATGAAATCCC TACGAAACCC GGTGAAAAGA AAGATGTCTC TGGGCCCCTG 
CCTCCTGCAT ACAGCCCCCG ATATGTTGAG GCTGCCTGGT ACCCGTGGTG GGTACGAGAG 
GGCTTCTTCA AACCAGAATA TCAGGCCCGG CTGCCCCAAG CTACAGGGGA GACCTTTTCC 
ATGTGTATCC CACCTCCCAA TGTCACTGGC TCCCTGCACA TTGGCCACGC ACTCACGGTG 
GCCATACAGG ATGCCCTCGT GCGCTGGCAC CGGATGCGTG GGGATCAAGT GCTGTGGGTC 
CCTGGTTCAG ATCATGCAGG AATTGCTACA CAAGCTGTGG TGGAGAAACA ACTGTGGAAG 
GAACGGGGAG TGAGGAGACA TGAGCTGAGC CGGGAGGCCT TCCTTAGGGA GGTGTGGCAG 
TGGAAGGAGG CGAAAGGTGG AGAGATCTGT GAGCAGCTGC GAGCTCTGGG TGCCTCCCTG 
GACTGGGATC GAGAGTGTTT TACCATGGAT GTTGGCTCCT CAGTGGCTGT GACTGAAGCT 
TTTGTGCAGC TCTACAAGGC GGGGTTGCTG TACCGGAACC ATCAGCTTGT CAACTGGTCA 
TGTGCTTTAA GATCAGCCAT CTCAGACATT GAGGTGGAGA ACCGGCCCCT GCCTGGCCAC 
ACACAGCTTC GACTGCCTGG CTGCCCCACC CCCGTGTCTT TTGGCCTCCT ATTTTCTATT 
GCCTTCCCGG TGGATGGAGA GCCTGATGCA GAGGTTGTGG TAGGAACCAC AAGGCCAGAG 
ACGCTGCCTG GAGATGTGGC TGTGGCCGTT CATCCAGACG ACTCGCGATA CACACATCTA 
CACGGGCGAC AGCTTCGTCA CCCCTTGATG GGGCAGCCTC TTCCCCTCAT CACAGACTAT 
GCTGTTCAGC CACATGTGGG CACGGGGGCA GTGAAGGTGA CTCCAGCTCA CAGTCCTGCC 
GATGCTGAGA TGGGGGCCCG ACATGGCTTG AGCCCCTTGA ATGTCATTGC GGAGGATGGG 
ACCATGACCT CCCTCTGCGG GGACTGGCTG CAGGGTCTTC ACCGGTTTGT GGCCCGGGAA 
AAGATAATGT CTGTGCTGAG TGAACGGGGC CTGTTCCGGG GCCTCCAGAA CCACCCCATG 
GTACTGCCCA TCTGCAGCCG TTCTGGGGAT GTGATAGAAT ACCTGCTGAA GAACCAGTGG 
TTTGTCCGCT GCCAGGAAAT GGGGGCCCGA GCTGCCAAGG CTGTGGAGTC GGGGGCCCTG 
GAGCTCAGTC CCTCCTTCCA CCAGAAGAAT TGGCAGCACT GGTTTTCCCA TATTGGGGAC 
TGGTGTGTCT CCCGGCAGCT GTGGTGGGGC CATCAGATTC CAGCCTACCT GGTTGTAGAG 
GACCATGCGC AGGGAGAAGA GGACTGTTGG GTGGTTGGGC GGTCAGAGGC TGAGGCCAGA 
GAGGTAGCAG CGGAACTGAC AGGGAGGCCA GGGGCAGAGC TGACCCTGGA GAGGGATCCT 
GATGTCCTAG ACACATGGTT TTCTTCTGCC CTGTTCCCCT TTTCTGCCCT GGGCTGGCCC 
CAAGAGACCC CAGACCTTGC TCGTTTCTAC CCCCTGTCAC TTTTGGAAAC GGGCAGTGAC 
CTTCTGCTGT TCTGGGTGGG CCGCATGGTC ATGTTGGGGA CCCAGCTCAC AGGGCAGCTG 
CCCTTCAGCA AGGTGCTTCT TCATCCCATG GTTCGGGACA GGCAGGGCCG GAAGATGAGC 
AAGTCCCTGG GGAATGTGCT GGACCCAAGA GACATCATCA GTGGGGTGGA GATGCAGGTG 
CTGCAGGAAA AGCTGAGAAG CGGAAATTTG GACCCTGCAG AGCTGGCCAT TGTGGCTGCA 
GCACAGAAAA AGGACTTTCC TCACGGGATC CCTGAGTGTG GGACAGATGC CCTGAGATTC 
ACACTCTGCT CCCATGGAGT TCAGGTGGGC GACTTGCACC TGTCAGTCTC TGAGGTCCAG 
AGCTGCCGAC ATTTCTGCAA CAAGATCTGG AATGCTCTTC GCTTTATCCT CAATGCTTTA 
GGGGAGAAAT TTGTGCCACA GCCTGCTGAG GAGCTGTCTC CCTCCTCCCC GATGGATGCC 
TGGATCTTGA GCCGCCTTGC CCTGGCTGCC CAGGAGTGTG AGCGGGGCTT CCTCACCCGA 
GAGCTCTCGC TCGTCACTCA TGCCCTGCAC CACTTCTGGC TTCACAACCT CTGTGACGTC 
TACCTGGAGG CTGTGAAGCC CGTGCTGTGG CACTCGCCCC GCCCCCTGGG GCCCCCTCAG 
GTCCTGTTCT CCTGCGCTGA CCTCGGCCTC CGCCTCCTGG CCCCACTGAT GCCCTTCCTG 
GCTGAAGAGC TCTGGCAGAG GCTGCCCCCC AGGGCTGGTT GCCCCCCTGC CCCCAGCATC 
TCGGTTGCCC CCTACCCCAG CGCCTGCAGC TTGGAGCACT GGCGCCAGCC AGAGCTGGAG 
CGGCGCTTCT CCCGGGTCCA AGAGGTCGTG CAGGTGCTAA GGGCTCTCCG AGCCACGTAC 
CAGCTCACCA AAGCCCGGCC CCGAGTGCTG CTGCAGAGCT CAGAGCCTGG GGACCAGGGC 
CTCTTCGAGG CCTTCTTGGA GCCCCTGGGC ACCCTGGGCT ACTGTGGGGC TGTGGGCCTG 
TTACCCCCAG GCGCAGCAGC TCCCTCCGGC TGGGCCCAGG CTCCACTCAG TGACACGGCT 
CAAGTCTACA TGGAGCTGCA GGGCCTGGTG GACCCGCAGA TCCAGCTACC TCTGTTAGCC 
GCCCGAAGGT ACAAGTTGCA GAAGCAGCTT GATAGCCTCA CAGCCAGGAC CCCATCAGAA 
GGGGAGGCAG GGACTCAGAG GCAACAAAAG CTTTCTTCCC TCCAGCTGGA ATTGTCAAAA 
CTGGACAAGG CAGCCTCTCA CCTCCGGCAG CTGATGGATG AGCCTCCAGC CCCAGGGAGC 
CCGGAGCTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VARS2 ( XM_016956716.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016812205.1 Pan troglodytes valyl-tRNA synthetase 2, mitochondrial (VARS2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OPc87417
Clone ID Related Accession (Same CDS sequence)	XM_016956717.1 , XM_016956716.2 , XM_016956716.1 , XM_016956718.1
Accession Version	XM_016956716.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3192bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	valine--tRNA ligase, mitochondrial isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036885.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_016956716.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTCATT TGCCTCTCGC CTCTTTTCGA CCACCATTTT GGGGGCTGAG GCACTCACGG 
GGCCTCCCCA GGTTTCACTC CGTTTCTACA CAGTCGGAGC CCCATGGATC TCCCATCTCC 
CGGAGGAACC GTGAAGCCAA ACAGAAGCGC CTGCGAGAGA AGCAGGCGAC TCTGGAGGCT 
GAGATAGCAG GGGAGAGCAA GTCACCTGCA GAATCCATTA AGGCCTGGAG GCCTAAGGAG 
TTAGTATTGT ATGAAATCCC TACGAAACCC GGTGAAAAGA AAGATGTCTC TGGGCCCCTG 
CCTCCTGCAT ACAGCCCCCG ATATGTTGAG GCTGCCTGGT ACCCGTGGTG GGTACGAGAG 
GGCTTCTTCA AACCAGAATA TCAGGCCCGG CTGCCCCAAG CTACAGGGGA GACCTTTTCC 
ATGTGTATCC CACCTCCCAA TGTCACTGGC TCCCTGCACA TTGGCCACGC ACTCACGGTG 
GCCATACAGG ATGCCCTCGT GCGCTGGCAC CGGATGCGTG GGGATCAAGT GCTGTGGGTC 
CCTGGTTCAG ATCATGCAGG AATTGCTACA CAAGCTGTGG TGGAGAAACA ACTGTGGAAG 
GAACGGGGAG TGAGGAGACA TGAGCTGAGC CGGGAGGCCT TCCTTAGGGA GGTGTGGCAG 
TGGAAGGAGG CGAAAGGTGG AGAGATCTGT GAGCAGCTGC GAGCTCTGGG TGCCTCCCTG 
GACTGGGATC GAGAGTGTTT TACCATGGAT GTTGGCTCCT CAGTGGCTGT GACTGAAGCT 
TTTGTGCAGC TCTACAAGGC GGGGTTGCTG TACCGGAACC ATCAGCTTGT CAACTGGTCA 
TGTGCTTTAA GATCAGCCAT CTCAGACATT GAGGTGGAGA ACCGGCCCCT GCCTGGCCAC 
ACACAGCTTC GACTGCCTGG CTGCCCCACC CCCGTGTCTT TTGGCCTCCT ATTTTCTATT 
GCCTTCCCGG TGGATGGAGA GCCTGATGCA GAGGTTGTGG TAGGAACCAC AAGGCCAGAG 
ACGCTGCCTG GAGATGTGGC TGTGGCCGTT CATCCAGACG ACTCGCGATA CACACATCTA 
CACGGGCGAC AGCTTCGTCA CCCCTTGATG GGGCAGCCTC TTCCCCTCAT CACAGACTAT 
GCTGTTCAGC CACATGTGGG CACGGGGGCA GTGAAGGTGA CTCCAGCTCA CAGTCCTGCC 
GATGCTGAGA TGGGGGCCCG ACATGGCTTG AGCCCCTTGA ATGTCATTGC GGAGGATGGG 
ACCATGACCT CCCTCTGCGG GGACTGGCTG CAGGGTCTTC ACCGGTTTGT GGCCCGGGAA 
AAGATAATGT CTGTGCTGAG TGAACGGGGC CTGTTCCGGG GCCTCCAGAA CCACCCCATG 
GTACTGCCCA TCTGCAGCCG TTCTGGGGAT GTGATAGAAT ACCTGCTGAA GAACCAGTGG 
TTTGTCCGCT GCCAGGAAAT GGGGGCCCGA GCTGCCAAGG CTGTGGAGTC GGGGGCCCTG 
GAGCTCAGTC CCTCCTTCCA CCAGAAGAAT TGGCAGCACT GGTTTTCCCA TATTGGGGAC 
TGGTGTGTCT CCCGGCAGCT GTGGTGGGGC CATCAGATTC CAGCCTACCT GGTTGTAGAG 
GACCATGCGC AGGGAGAAGA GGACTGTTGG GTGGTTGGGC GGTCAGAGGC TGAGGCCAGA 
GAGGTAGCAG CGGAACTGAC AGGGAGGCCA GGGGCAGAGC TGACCCTGGA GAGGGATCCT 
GATGTCCTAG ACACATGGTT TTCTTCTGCC CTGTTCCCCT TTTCTGCCCT GGGCTGGCCC 
CAAGAGACCC CAGACCTTGC TCGTTTCTAC CCCCTGTCAC TTTTGGAAAC GGGCAGTGAC 
CTTCTGCTGT TCTGGGTGGG CCGCATGGTC ATGTTGGGGA CCCAGCTCAC AGGGCAGCTG 
CCCTTCAGCA AGGTGCTTCT TCATCCCATG GTTCGGGACA GGCAGGGCCG GAAGATGAGC 
AAGTCCCTGG GGAATGTGCT GGACCCAAGA GACATCATCA GTGGGGTGGA GATGCAGGTG 
CTGCAGGAAA AGCTGAGAAG CGGAAATTTG GACCCTGCAG AGCTGGCCAT TGTGGCTGCA 
GCACAGAAAA AGGACTTTCC TCACGGGATC CCTGAGTGTG GGACAGATGC CCTGAGATTC 
ACACTCTGCT CCCATGGAGT TCAGGTGGGC GACTTGCACC TGTCAGTCTC TGAGGTCCAG 
AGCTGCCGAC ATTTCTGCAA CAAGATCTGG AATGCTCTTC GCTTTATCCT CAATGCTTTA 
GGGGAGAAAT TTGTGCCACA GCCTGCTGAG GAGCTGTCTC CCTCCTCCCC GATGGATGCC 
TGGATCTTGA GCCGCCTTGC CCTGGCTGCC CAGGAGTGTG AGCGGGGCTT CCTCACCCGA 
GAGCTCTCGC TCGTCACTCA TGCCCTGCAC CACTTCTGGC TTCACAACCT CTGTGACGTC 
TACCTGGAGG CTGTGAAGCC CGTGCTGTGG CACTCGCCCC GCCCCCTGGG GCCCCCTCAG 
GTCCTGTTCT CCTGCGCTGA CCTCGGCCTC CGCCTCCTGG CCCCACTGAT GCCCTTCCTG 
GCTGAAGAGC TCTGGCAGAG GCTGCCCCCC AGGGCTGGTT GCCCCCCTGC CCCCAGCATC 
TCGGTTGCCC CCTACCCCAG CGCCTGCAGC TTGGAGCACT GGCGCCAGCC AGAGCTGGAG 
CGGCGCTTCT CCCGGGTCCA AGAGGTCGTG CAGGTGCTAA GGGCTCTCCG AGCCACGTAC 
CAGCTCACCA AAGCCCGGCC CCGAGTGCTG CTGCAGAGCT CAGAGCCTGG GGACCAGGGC 
CTCTTCGAGG CCTTCTTGGA GCCCCTGGGC ACCCTGGGCT ACTGTGGGGC TGTGGGCCTG 
TTACCCCCAG GCGCAGCAGC TCCCTCCGGC TGGGCCCAGG CTCCACTCAG TGACACGGCT 
CAAGTCTACA TGGAGCTGCA GGGCCTGGTG GACCCGCAGA TCCAGCTACC TCTGTTAGCC 
GCCCGAAGGT ACAAGTTGCA GAAGCAGCTT GATAGCCTCA CAGCCAGGAC CCCATCAGAA 
GGGGAGGCAG GGACTCAGAG GCAACAAAAG CTTTCTTCCC TCCAGCTGGA ATTGTCAAAA 
CTGGACAAGG CAGCCTCTCA CCTCCGGCAG CTGATGGATG AGCCTCCAGC CCCAGGGAGC 
CCGGAGCTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016812205.1
CDS	188..3379
Translation	MPHLPLASFRPPFWGLRHSRGLPRFHSVSTQSEPHGSPISRRNREAKQKRLREKQATLEAEIAGESKSPAESIKAWRPKELVLYEIPTKPGEKKDVSGPLPPAYSPRYVEAAWYPWWVREGFFKPEYQARLPQATGETFSMCIPPPNVTGSLHIGHALTVAIQDALVRWHRMRGDQVLWVPGSDHAGIATQAVVEKQLWKERGVRRHELSREAFLREVWQWKEAKGGEICEQLRALGASLDWDRECFTMDVGSSVAVTEAFVQLYKAGLLYRNHQLVNWSCALRSAISDIEVENRPLPGHTQLRLPGCPTPVSFGLLFSIAFPVDGEPDAEVVVGTTRPETLPGDVAVAVHPDDSRYTHLHGRQLRHPLMGQPLPLITDYAVQPHVGTGAVKVTPAHSPADAEMGARHGLSPLNVIAEDGTMTSLCGDWLQGLHRFVAREKIMSVLSERGLFRGLQNHPMVLPICSRSGDVIEYLLKNQWFVRCQEMGARAAKAVESGALELSPSFHQKNWQHWFSHIGDWCVSRQLWWGHQIPAYLVVEDHAQGEEDCWVVGRSEAEAREVAAELTGRPGAELTLERDPDVLDTWFSSALFPFSALGWPQETPDLARFYPLSLLETGSDLLLFWVGRMVMLGTQLTGQLPFSKVLLHPMVRDRQGRKMSKSLGNVLDPRDIISGVEMQVLQEKLRSGNLDPAELAIVAAAQKKDFPHGIPECGTDALRFTLCSHGVQVGDLHLSVSEVQSCRHFCNKIWNALRFILNALGEKFVPQPAEELSPSSPMDAWILSRLALAAQECERGFLTRELSLVTHALHHFWLHNLCDVYLEAVKPVLWHSPRPLGPPQVLFSCADLGLRLLAPLMPFLAEELWQRLPPRAGCPPAPSISVAPYPSACSLEHWRQPELERRFSRVQEVVQVLRALRATYQLTKARPRVLLQSSEPGDQGLFEAFLEPLGTLGYCGAVGLLPPGAAAPSGWAQAPLSDTAQVYMELQGLVDPQIQLPLLAARRYKLQKQLDSLTARTPSEGEAGTQRQQKLSSLQLELSKLDKAASHLRQLMDEPPAPGSPEL

Target ORF information:

RefSeq Version	XM_016956716.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes valyl-tRNA synthetase 2, mitochondrial (VARS2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016956716.2

ORF Insert Sequence:

ATGCCTCATT TGCCTCTCGC CTCTTTTCGA CCACCATTTT GGGGGCTGAG GCACTCACGG 
GGCCTCCCCA GGTTTCACTC CGTTTCTACA CAGTCGGAGC CCCATGGATC TCCCATCTCC 
CGGAGGAACC GTGAAGCCAA ACAGAAGCGC CTGCGAGAGA AGCAGGCGAC TCTGGAGGCT 
GAGATAGCAG GGGAGAGCAA GTCACCTGCA GAATCCATTA AGGCCTGGAG GCCTAAGGAG 
TTAGTATTGT ATGAAATCCC TACGAAACCC GGTGAAAAGA AAGATGTCTC TGGGCCCCTG 
CCTCCTGCAT ACAGCCCCCG ATATGTTGAG GCTGCCTGGT ACCCGTGGTG GGTACGAGAG 
GGCTTCTTCA AACCAGAATA TCAGGCCCGG CTGCCCCAAG CTACAGGGGA GACCTTTTCC 
ATGTGTATCC CACCTCCCAA TGTCACTGGC TCCCTGCACA TTGGCCACGC ACTCACGGTG 
GCCATACAGG ATGCCCTCGT GCGCTGGCAC CGGATGCGTG GGGATCAAGT GCTGTGGGTC 
CCTGGTTCAG ATCATGCAGG AATTGCTACA CAAGCTGTGG TGGAGAAACA ACTGTGGAAG 
GAACGGGGAG TGAGGAGACA TGAGCTGAGC CGGGAGGCCT TCCTTAGGGA GGTGTGGCAG 
TGGAAGGAGG CGAAAGGTGG AGAGATCTGT GAGCAGCTGC GAGCTCTGGG TGCCTCCCTG 
GACTGGGATC GAGAGTGTTT TACCATGGAT GTTGGCTCCT CAGTGGCTGT GACTGAAGCT 
TTTGTGCAGC TCTACAAGGC GGGGTTGCTG TACCGGAACC ATCAGCTTGT CAACTGGTCA 
TGTGCTTTAA GATCAGCCAT CTCAGACATT GAGGTGGAGA ACCGGCCCCT GCCTGGCCAC 
ACACAGCTTC GACTGCCTGG CTGCCCCACC CCCGTGTCTT TTGGCCTCCT ATTTTCTATT 
GCCTTCCCGG TGGATGGAGA GCCTGATGCA GAGGTTGTGG TAGGAACCAC AAGGCCAGAG 
ACGCTGCCTG GAGATGTGGC TGTGGCCGTT CATCCAGACG ACTCGCGATA CACACATCTA 
CACGGGCGAC AGCTTCGTCA CCCCTTGATG GGGCAGCCTC TTCCCCTCAT CACAGACTAT 
GCTGTTCAGC CACATGTGGG CACGGGGGCA GTGAAGGTGA CTCCAGCTCA CAGTCCTGCC 
GATGCTGAGA TGGGGGCCCG ACATGGCTTG AGCCCCTTGA ATGTCATTGC GGAGGATGGG 
ACCATGACCT CCCTCTGCGG GGACTGGCTG CAGGGTCTTC ACCGGTTTGT GGCCCGGGAA 
AAGATAATGT CTGTGCTGAG TGAACGGGGC CTGTTCCGGG GCCTCCAGAA CCACCCCATG 
GTACTGCCCA TCTGCAGCCG TTCTGGGGAT GTGATAGAAT ACCTGCTGAA GAACCAGTGG 
TTTGTCCGCT GCCAGGAAAT GGGGGCCCGA GCTGCCAAGG CTGTGGAGTC GGGGGCCCTG 
GAGCTCAGTC CCTCCTTCCA CCAGAAGAAT TGGCAGCACT GGTTTTCCCA TATTGGGGAC 
TGGTGTGTCT CCCGGCAGCT GTGGTGGGGC CATCAGATTC CAGCCTACCT GGTTGTAGAG 
GACCATGCGC AGGGAGAAGA GGACTGTTGG GTGGTTGGGC GGTCAGAGGC TGAGGCCAGA 
GAGGTAGCAG CGGAACTGAC AGGGAGGCCA GGGGCAGAGC TGACCCTGGA GAGGGATCCT 
GATGTCCTAG ACACATGGTT TTCTTCTGCC CTGTTCCCCT TTTCTGCCCT GGGCTGGCCC 
CAAGAGACCC CAGACCTTGC TCGTTTCTAC CCCCTGTCAC TTTTGGAAAC GGGCAGTGAC 
CTTCTGCTGT TCTGGGTGGG CCGCATGGTC ATGTTGGGGA CCCAGCTCAC AGGGCAGCTG 
CCCTTCAGCA AGGTGCTTCT TCATCCCATG GTTCGGGACA GGCAGGGCCG GAAGATGAGC 
AAGTCCCTGG GGAATGTGCT GGACCCAAGA GACATCATCA GTGGGGTGGA GATGCAGGTG 
CTGCAGGAAA AGCTGAGAAG CGGAAATTTG GACCCTGCAG AGCTGGCCAT TGTGGCTGCA 
GCACAGAAAA AGGACTTTCC TCACGGGATC CCTGAGTGTG GGACAGATGC CCTGAGATTC 
ACACTCTGCT CCCATGGAGT TCAGGTGGGC GACTTGCACC TGTCAGTCTC TGAGGTCCAG 
AGCTGCCGAC ATTTCTGCAA CAAGATCTGG AATGCTCTTC GCTTTATCCT CAATGCTTTA 
GGGGAGAAAT TTGTGCCACA GCCTGCTGAG GAGCTGTCTC CCTCCTCCCC GATGGATGCC 
TGGATCTTGA GCCGCCTTGC CCTGGCTGCC CAGGAGTGTG AGCGGGGCTT CCTCACCCGA 
GAGCTCTCGC TCGTCACTCA TGCCCTGCAC CACTTCTGGC TTCACAACCT CTGTGACGTC 
TACCTGGAGG CTGTGAAGCC CGTGCTGTGG CACTCGCCCC GCCCCCTGGG GCCCCCTCAG 
GTCCTGTTCT CCTGCGCTGA CCTCGGCCTC CGCCTCCTGG CCCCACTGAT GCCCTTCCTG 
GCTGAAGAGC TCTGGCAGAG GCTGCCCCCC AGGGCTGGTT GCCCCCCTGC CCCCAGCATC 
TCGGTTGCCC CCTACCCCAG CGCCTGCAGC TTGGAGCACT GGCGCCAGCC AGAGCTGGAG 
CGGCGCTTCT CCCGGGTCCA AGAGGTCGTG CAGGTGCTAA GGGCTCTCCG AGCCACGTAC 
CAGCTCACCA AAGCCCGGCC CCGAGTGCTG CTGCAGAGCT CAGAGCCTGG GGACCAGGGC 
CTCTTCGAGG CCTTCTTGGA GCCCCTGGGC ACCCTGGGCT ACTGTGGGGC TGTGGGCCTG 
TTACCCCCAG GCGCAGCAGC TCCCTCCGGC TGGGCCCAGG CTCCACTCAG TGACACGGCT 
CAAGTCTACA TGGAGCTGCA GGGCCTGGTG GACCCGCAGA TCCAGCTACC TCTGTTAGCC 
GCCCGAAGGT ACAAGTTGCA GAAGCAGCTT GATAGCCTCA CAGCCAGGAC CCCATCAGAA 
GGGGAGGCAG GGACTCAGAG GCAACAAAAG CTTTCTTCCC TCCAGCTGGA ATTGTCAAAA 
CTGGACAAGG CAGCCTCTCA CCTCCGGCAG CTGATGGATG AGCCTCCAGC CCCAGGGAGC 
CCGGAGCTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VARS2 ( XM_016956716.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016812205.1 Pan troglodytes valyl-tRNA synthetase 2, mitochondrial (VARS2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OPc87417
Clone ID Related Accession (Same CDS sequence)	XM_016956717.1 , XM_016956716.2 , XM_016956716.1 , XM_016956718.1
Accession Version	XM_016956716.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3192bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	valine--tRNA ligase, mitochondrial isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006473.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTCATT TGCCTCTCGC CTCTTTTCGA CCACCATTTT GGGGGCTGAG GCACTCACGG 
GGCCTCCCCA GGTTTCACTC CGTTTCTACA CAGTCGGAGC CCCATGGATC TCCCATCTCC 
CGGAGGAACC GTGAAGCCAA ACAGAAGCGC CTGCGAGAGA AGCAGGCGAC TCTGGAGGCT 
GAGATAGCAG GGGAGAGCAA GTCACCTGCA GAATCCATTA AGGCCTGGAG GCCTAAGGAG 
TTAGTATTGT ATGAAATCCC TACGAAACCC GGTGAAAAGA AAGATGTCTC TGGGCCCCTG 
CCTCCTGCAT ACAGCCCCCG ATATGTTGAG GCTGCCTGGT ACCCGTGGTG GGTACGAGAG 
GGCTTCTTCA AACCAGAATA TCAGGCCCGG CTGCCCCAAG CTACAGGGGA GACCTTTTCC 
ATGTGTATCC CACCTCCCAA TGTCACTGGC TCCCTGCACA TTGGCCACGC ACTCACGGTG 
GCCATACAGG ATGCCCTCGT GCGCTGGCAC CGGATGCGTG GGGATCAAGT GCTGTGGGTC 
CCTGGTTCAG ATCATGCAGG AATTGCTACA CAAGCTGTGG TGGAGAAACA ACTGTGGAAG 
GAACGGGGAG TGAGGAGACA TGAGCTGAGC CGGGAGGCCT TCCTTAGGGA GGTGTGGCAG 
TGGAAGGAGG CGAAAGGTGG AGAGATCTGT GAGCAGCTGC GAGCTCTGGG TGCCTCCCTG 
GACTGGGATC GAGAGTGTTT TACCATGGAT GTTGGCTCCT CAGTGGCTGT GACTGAAGCT 
TTTGTGCAGC TCTACAAGGC GGGGTTGCTG TACCGGAACC ATCAGCTTGT CAACTGGTCA 
TGTGCTTTAA GATCAGCCAT CTCAGACATT GAGGTGGAGA ACCGGCCCCT GCCTGGCCAC 
ACACAGCTTC GACTGCCTGG CTGCCCCACC CCCGTGTCTT TTGGCCTCCT ATTTTCTATT 
GCCTTCCCGG TGGATGGAGA GCCTGATGCA GAGGTTGTGG TAGGAACCAC AAGGCCAGAG 
ACGCTGCCTG GAGATGTGGC TGTGGCCGTT CATCCAGACG ACTCGCGATA CACACATCTA 
CACGGGCGAC AGCTTCGTCA CCCCTTGATG GGGCAGCCTC TTCCCCTCAT CACAGACTAT 
GCTGTTCAGC CACATGTGGG CACGGGGGCA GTGAAGGTGA CTCCAGCTCA CAGTCCTGCC 
GATGCTGAGA TGGGGGCCCG ACATGGCTTG AGCCCCTTGA ATGTCATTGC GGAGGATGGG 
ACCATGACCT CCCTCTGCGG GGACTGGCTG CAGGGTCTTC ACCGGTTTGT GGCCCGGGAA 
AAGATAATGT CTGTGCTGAG TGAACGGGGC CTGTTCCGGG GCCTCCAGAA CCACCCCATG 
GTACTGCCCA TCTGCAGCCG TTCTGGGGAT GTGATAGAAT ACCTGCTGAA GAACCAGTGG 
TTTGTCCGCT GCCAGGAAAT GGGGGCCCGA GCTGCCAAGG CTGTGGAGTC GGGGGCCCTG 
GAGCTCAGTC CCTCCTTCCA CCAGAAGAAT TGGCAGCACT GGTTTTCCCA TATTGGGGAC 
TGGTGTGTCT CCCGGCAGCT GTGGTGGGGC CATCAGATTC CAGCCTACCT GGTTGTAGAG 
GACCATGCGC AGGGAGAAGA GGACTGTTGG GTGGTTGGGC GGTCAGAGGC TGAGGCCAGA 
GAGGTAGCAG CGGAACTGAC AGGGAGGCCA GGGGCAGAGC TGACCCTGGA GAGGGATCCT 
GATGTCCTAG ACACATGGTT TTCTTCTGCC CTGTTCCCCT TTTCTGCCCT GGGCTGGCCC 
CAAGAGACCC CAGACCTTGC TCGTTTCTAC CCCCTGTCAC TTTTGGAAAC GGGCAGTGAC 
CTTCTGCTGT TCTGGGTGGG CCGCATGGTC ATGTTGGGGA CCCAGCTCAC AGGGCAGCTG 
CCCTTCAGCA AGGTGCTTCT TCATCCCATG GTTCGGGACA GGCAGGGCCG GAAGATGAGC 
AAGTCCCTGG GGAATGTGCT GGACCCAAGA GACATCATCA GTGGGGTGGA GATGCAGGTG 
CTGCAGGAAA AGCTGAGAAG CGGAAATTTG GACCCTGCAG AGCTGGCCAT TGTGGCTGCA 
GCACAGAAAA AGGACTTTCC TCACGGGATC CCTGAGTGTG GGACAGATGC CCTGAGATTC 
ACACTCTGCT CCCATGGAGT TCAGGTGGGC GACTTGCACC TGTCAGTCTC TGAGGTCCAG 
AGCTGCCGAC ATTTCTGCAA CAAGATCTGG AATGCTCTTC GCTTTATCCT CAATGCTTTA 
GGGGAGAAAT TTGTGCCACA GCCTGCTGAG GAGCTGTCTC CCTCCTCCCC GATGGATGCC 
TGGATCTTGA GCCGCCTTGC CCTGGCTGCC CAGGAGTGTG AGCGGGGCTT CCTCACCCGA 
GAGCTCTCGC TCGTCACTCA TGCCCTGCAC CACTTCTGGC TTCACAACCT CTGTGACGTC 
TACCTGGAGG CTGTGAAGCC CGTGCTGTGG CACTCGCCCC GCCCCCTGGG GCCCCCTCAG 
GTCCTGTTCT CCTGCGCTGA CCTCGGCCTC CGCCTCCTGG CCCCACTGAT GCCCTTCCTG 
GCTGAAGAGC TCTGGCAGAG GCTGCCCCCC AGGGCTGGTT GCCCCCCTGC CCCCAGCATC 
TCGGTTGCCC CCTACCCCAG CGCCTGCAGC TTGGAGCACT GGCGCCAGCC AGAGCTGGAG 
CGGCGCTTCT CCCGGGTCCA AGAGGTCGTG CAGGTGCTAA GGGCTCTCCG AGCCACGTAC 
CAGCTCACCA AAGCCCGGCC CCGAGTGCTG CTGCAGAGCT CAGAGCCTGG GGACCAGGGC 
CTCTTCGAGG CCTTCTTGGA GCCCCTGGGC ACCCTGGGCT ACTGTGGGGC TGTGGGCCTG 
TTACCCCCAG GCGCAGCAGC TCCCTCCGGC TGGGCCCAGG CTCCACTCAG TGACACGGCT 
CAAGTCTACA TGGAGCTGCA GGGCCTGGTG GACCCGCAGA TCCAGCTACC TCTGTTAGCC 
GCCCGAAGGT ACAAGTTGCA GAAGCAGCTT GATAGCCTCA CAGCCAGGAC CCCATCAGAA 
GGGGAGGCAG GGACTCAGAG GCAACAAAAG CTTTCTTCCC TCCAGCTGGA ATTGTCAAAA 
CTGGACAAGG CAGCCTCTCA CCTCCGGCAG CTGATGGATG AGCCTCCAGC CCCAGGGAGC 
CCGGAGCTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016812205.1
CDS	189..3380
Translation	MPHLPLASFRPPFWGLRHSRGLPRFHSVSTQSEPHGSPISRRNREAKQKRLREKQATLEAEIAGESKSPAESIKAWRPKELVLYEIPTKPGEKKDVSGPLPPAYSPRYVEAAWYPWWVREGFFKPEYQARLPQATGETFSMCIPPPNVTGSLHIGHALTVAIQDALVRWHRMRGDQVLWVPGSDHAGIATQAVVEKQLWKERGVRRHELSREAFLREVWQWKEAKGGEICEQLRALGASLDWDRECFTMDVGSSVAVTEAFVQLYKAGLLYRNHQLVNWSCALRSAISDIEVENRPLPGHTQLRLPGCPTPVSFGLLFSIAFPVDGEPDAEVVVGTTRPETLPGDVAVAVHPDDSRYTHLHGRQLRHPLMGQPLPLITDYAVQPHVGTGAVKVTPAHSPADAEMGARHGLSPLNVIAEDGTMTSLCGDWLQGLHRFVAREKIMSVLSERGLFRGLQNHPMVLPICSRSGDVIEYLLKNQWFVRCQEMGARAAKAVESGALELSPSFHQKNWQHWFSHIGDWCVSRQLWWGHQIPAYLVVEDHAQGEEDCWVVGRSEAEAREVAAELTGRPGAELTLERDPDVLDTWFSSALFPFSALGWPQETPDLARFYPLSLLETGSDLLLFWVGRMVMLGTQLTGQLPFSKVLLHPMVRDRQGRKMSKSLGNVLDPRDIISGVEMQVLQEKLRSGNLDPAELAIVAAAQKKDFPHGIPECGTDALRFTLCSHGVQVGDLHLSVSEVQSCRHFCNKIWNALRFILNALGEKFVPQPAEELSPSSPMDAWILSRLALAAQECERGFLTRELSLVTHALHHFWLHNLCDVYLEAVKPVLWHSPRPLGPPQVLFSCADLGLRLLAPLMPFLAEELWQRLPPRAGCPPAPSISVAPYPSACSLEHWRQPELERRFSRVQEVVQVLRALRATYQLTKARPRVLLQSSEPGDQGLFEAFLEPLGTLGYCGAVGLLPPGAAAPSGWAQAPLSDTAQVYMELQGLVDPQIQLPLLAARRYKLQKQLDSLTARTPSEGEAGTQRQQKLSSLQLELSKLDKAASHLRQLMDEPPAPGSPEL

Target ORF information:

RefSeq Version	XM_016956716.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes valyl-tRNA synthetase 2, mitochondrial (VARS2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016956716.1

ORF Insert Sequence:

ATGCCTCATT TGCCTCTCGC CTCTTTTCGA CCACCATTTT GGGGGCTGAG GCACTCACGG 
GGCCTCCCCA GGTTTCACTC CGTTTCTACA CAGTCGGAGC CCCATGGATC TCCCATCTCC 
CGGAGGAACC GTGAAGCCAA ACAGAAGCGC CTGCGAGAGA AGCAGGCGAC TCTGGAGGCT 
GAGATAGCAG GGGAGAGCAA GTCACCTGCA GAATCCATTA AGGCCTGGAG GCCTAAGGAG 
TTAGTATTGT ATGAAATCCC TACGAAACCC GGTGAAAAGA AAGATGTCTC TGGGCCCCTG 
CCTCCTGCAT ACAGCCCCCG ATATGTTGAG GCTGCCTGGT ACCCGTGGTG GGTACGAGAG 
GGCTTCTTCA AACCAGAATA TCAGGCCCGG CTGCCCCAAG CTACAGGGGA GACCTTTTCC 
ATGTGTATCC CACCTCCCAA TGTCACTGGC TCCCTGCACA TTGGCCACGC ACTCACGGTG 
GCCATACAGG ATGCCCTCGT GCGCTGGCAC CGGATGCGTG GGGATCAAGT GCTGTGGGTC 
CCTGGTTCAG ATCATGCAGG AATTGCTACA CAAGCTGTGG TGGAGAAACA ACTGTGGAAG 
GAACGGGGAG TGAGGAGACA TGAGCTGAGC CGGGAGGCCT TCCTTAGGGA GGTGTGGCAG 
TGGAAGGAGG CGAAAGGTGG AGAGATCTGT GAGCAGCTGC GAGCTCTGGG TGCCTCCCTG 
GACTGGGATC GAGAGTGTTT TACCATGGAT GTTGGCTCCT CAGTGGCTGT GACTGAAGCT 
TTTGTGCAGC TCTACAAGGC GGGGTTGCTG TACCGGAACC ATCAGCTTGT CAACTGGTCA 
TGTGCTTTAA GATCAGCCAT CTCAGACATT GAGGTGGAGA ACCGGCCCCT GCCTGGCCAC 
ACACAGCTTC GACTGCCTGG CTGCCCCACC CCCGTGTCTT TTGGCCTCCT ATTTTCTATT 
GCCTTCCCGG TGGATGGAGA GCCTGATGCA GAGGTTGTGG TAGGAACCAC AAGGCCAGAG 
ACGCTGCCTG GAGATGTGGC TGTGGCCGTT CATCCAGACG ACTCGCGATA CACACATCTA 
CACGGGCGAC AGCTTCGTCA CCCCTTGATG GGGCAGCCTC TTCCCCTCAT CACAGACTAT 
GCTGTTCAGC CACATGTGGG CACGGGGGCA GTGAAGGTGA CTCCAGCTCA CAGTCCTGCC 
GATGCTGAGA TGGGGGCCCG ACATGGCTTG AGCCCCTTGA ATGTCATTGC GGAGGATGGG 
ACCATGACCT CCCTCTGCGG GGACTGGCTG CAGGGTCTTC ACCGGTTTGT GGCCCGGGAA 
AAGATAATGT CTGTGCTGAG TGAACGGGGC CTGTTCCGGG GCCTCCAGAA CCACCCCATG 
GTACTGCCCA TCTGCAGCCG TTCTGGGGAT GTGATAGAAT ACCTGCTGAA GAACCAGTGG 
TTTGTCCGCT GCCAGGAAAT GGGGGCCCGA GCTGCCAAGG CTGTGGAGTC GGGGGCCCTG 
GAGCTCAGTC CCTCCTTCCA CCAGAAGAAT TGGCAGCACT GGTTTTCCCA TATTGGGGAC 
TGGTGTGTCT CCCGGCAGCT GTGGTGGGGC CATCAGATTC CAGCCTACCT GGTTGTAGAG 
GACCATGCGC AGGGAGAAGA GGACTGTTGG GTGGTTGGGC GGTCAGAGGC TGAGGCCAGA 
GAGGTAGCAG CGGAACTGAC AGGGAGGCCA GGGGCAGAGC TGACCCTGGA GAGGGATCCT 
GATGTCCTAG ACACATGGTT TTCTTCTGCC CTGTTCCCCT TTTCTGCCCT GGGCTGGCCC 
CAAGAGACCC CAGACCTTGC TCGTTTCTAC CCCCTGTCAC TTTTGGAAAC GGGCAGTGAC 
CTTCTGCTGT TCTGGGTGGG CCGCATGGTC ATGTTGGGGA CCCAGCTCAC AGGGCAGCTG 
CCCTTCAGCA AGGTGCTTCT TCATCCCATG GTTCGGGACA GGCAGGGCCG GAAGATGAGC 
AAGTCCCTGG GGAATGTGCT GGACCCAAGA GACATCATCA GTGGGGTGGA GATGCAGGTG 
CTGCAGGAAA AGCTGAGAAG CGGAAATTTG GACCCTGCAG AGCTGGCCAT TGTGGCTGCA 
GCACAGAAAA AGGACTTTCC TCACGGGATC CCTGAGTGTG GGACAGATGC CCTGAGATTC 
ACACTCTGCT CCCATGGAGT TCAGGTGGGC GACTTGCACC TGTCAGTCTC TGAGGTCCAG 
AGCTGCCGAC ATTTCTGCAA CAAGATCTGG AATGCTCTTC GCTTTATCCT CAATGCTTTA 
GGGGAGAAAT TTGTGCCACA GCCTGCTGAG GAGCTGTCTC CCTCCTCCCC GATGGATGCC 
TGGATCTTGA GCCGCCTTGC CCTGGCTGCC CAGGAGTGTG AGCGGGGCTT CCTCACCCGA 
GAGCTCTCGC TCGTCACTCA TGCCCTGCAC CACTTCTGGC TTCACAACCT CTGTGACGTC 
TACCTGGAGG CTGTGAAGCC CGTGCTGTGG CACTCGCCCC GCCCCCTGGG GCCCCCTCAG 
GTCCTGTTCT CCTGCGCTGA CCTCGGCCTC CGCCTCCTGG CCCCACTGAT GCCCTTCCTG 
GCTGAAGAGC TCTGGCAGAG GCTGCCCCCC AGGGCTGGTT GCCCCCCTGC CCCCAGCATC 
TCGGTTGCCC CCTACCCCAG CGCCTGCAGC TTGGAGCACT GGCGCCAGCC AGAGCTGGAG 
CGGCGCTTCT CCCGGGTCCA AGAGGTCGTG CAGGTGCTAA GGGCTCTCCG AGCCACGTAC 
CAGCTCACCA AAGCCCGGCC CCGAGTGCTG CTGCAGAGCT CAGAGCCTGG GGACCAGGGC 
CTCTTCGAGG CCTTCTTGGA GCCCCTGGGC ACCCTGGGCT ACTGTGGGGC TGTGGGCCTG 
TTACCCCCAG GCGCAGCAGC TCCCTCCGGC TGGGCCCAGG CTCCACTCAG TGACACGGCT 
CAAGTCTACA TGGAGCTGCA GGGCCTGGTG GACCCGCAGA TCCAGCTACC TCTGTTAGCC 
GCCCGAAGGT ACAAGTTGCA GAAGCAGCTT GATAGCCTCA CAGCCAGGAC CCCATCAGAA 
GGGGAGGCAG GGACTCAGAG GCAACAAAAG CTTTCTTCCC TCCAGCTGGA ATTGTCAAAA 
CTGGACAAGG CAGCCTCTCA CCTCCGGCAG CTGATGGATG AGCCTCCAGC CCCAGGGAGC 
CCGGAGCTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VARS2 ( XM_016956718.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016812207.1 Pan troglodytes valyl-tRNA synthetase 2, mitochondrial (VARS2), transcript variant X5, mRNA.

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CloneID	OPc87417
Clone ID Related Accession (Same CDS sequence)	XM_016956717.1 , XM_016956716.2 , XM_016956716.1 , XM_016956718.1
Accession Version	XM_016956718.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3192bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	valine--tRNA ligase, mitochondrial isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036885.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTCATT TGCCTCTCGC CTCTTTTCGA CCACCATTTT GGGGGCTGAG GCACTCACGG 
GGCCTCCCCA GGTTTCACTC CGTTTCTACA CAGTCGGAGC CCCATGGATC TCCCATCTCC 
CGGAGGAACC GTGAAGCCAA ACAGAAGCGC CTGCGAGAGA AGCAGGCGAC TCTGGAGGCT 
GAGATAGCAG GGGAGAGCAA GTCACCTGCA GAATCCATTA AGGCCTGGAG GCCTAAGGAG 
TTAGTATTGT ATGAAATCCC TACGAAACCC GGTGAAAAGA AAGATGTCTC TGGGCCCCTG 
CCTCCTGCAT ACAGCCCCCG ATATGTTGAG GCTGCCTGGT ACCCGTGGTG GGTACGAGAG 
GGCTTCTTCA AACCAGAATA TCAGGCCCGG CTGCCCCAAG CTACAGGGGA GACCTTTTCC 
ATGTGTATCC CACCTCCCAA TGTCACTGGC TCCCTGCACA TTGGCCACGC ACTCACGGTG 
GCCATACAGG ATGCCCTCGT GCGCTGGCAC CGGATGCGTG GGGATCAAGT GCTGTGGGTC 
CCTGGTTCAG ATCATGCAGG AATTGCTACA CAAGCTGTGG TGGAGAAACA ACTGTGGAAG 
GAACGGGGAG TGAGGAGACA TGAGCTGAGC CGGGAGGCCT TCCTTAGGGA GGTGTGGCAG 
TGGAAGGAGG CGAAAGGTGG AGAGATCTGT GAGCAGCTGC GAGCTCTGGG TGCCTCCCTG 
GACTGGGATC GAGAGTGTTT TACCATGGAT GTTGGCTCCT CAGTGGCTGT GACTGAAGCT 
TTTGTGCAGC TCTACAAGGC GGGGTTGCTG TACCGGAACC ATCAGCTTGT CAACTGGTCA 
TGTGCTTTAA GATCAGCCAT CTCAGACATT GAGGTGGAGA ACCGGCCCCT GCCTGGCCAC 
ACACAGCTTC GACTGCCTGG CTGCCCCACC CCCGTGTCTT TTGGCCTCCT ATTTTCTATT 
GCCTTCCCGG TGGATGGAGA GCCTGATGCA GAGGTTGTGG TAGGAACCAC AAGGCCAGAG 
ACGCTGCCTG GAGATGTGGC TGTGGCCGTT CATCCAGACG ACTCGCGATA CACACATCTA 
CACGGGCGAC AGCTTCGTCA CCCCTTGATG GGGCAGCCTC TTCCCCTCAT CACAGACTAT 
GCTGTTCAGC CACATGTGGG CACGGGGGCA GTGAAGGTGA CTCCAGCTCA CAGTCCTGCC 
GATGCTGAGA TGGGGGCCCG ACATGGCTTG AGCCCCTTGA ATGTCATTGC GGAGGATGGG 
ACCATGACCT CCCTCTGCGG GGACTGGCTG CAGGGTCTTC ACCGGTTTGT GGCCCGGGAA 
AAGATAATGT CTGTGCTGAG TGAACGGGGC CTGTTCCGGG GCCTCCAGAA CCACCCCATG 
GTACTGCCCA TCTGCAGCCG TTCTGGGGAT GTGATAGAAT ACCTGCTGAA GAACCAGTGG 
TTTGTCCGCT GCCAGGAAAT GGGGGCCCGA GCTGCCAAGG CTGTGGAGTC GGGGGCCCTG 
GAGCTCAGTC CCTCCTTCCA CCAGAAGAAT TGGCAGCACT GGTTTTCCCA TATTGGGGAC 
TGGTGTGTCT CCCGGCAGCT GTGGTGGGGC CATCAGATTC CAGCCTACCT GGTTGTAGAG 
GACCATGCGC AGGGAGAAGA GGACTGTTGG GTGGTTGGGC GGTCAGAGGC TGAGGCCAGA 
GAGGTAGCAG CGGAACTGAC AGGGAGGCCA GGGGCAGAGC TGACCCTGGA GAGGGATCCT 
GATGTCCTAG ACACATGGTT TTCTTCTGCC CTGTTCCCCT TTTCTGCCCT GGGCTGGCCC 
CAAGAGACCC CAGACCTTGC TCGTTTCTAC CCCCTGTCAC TTTTGGAAAC GGGCAGTGAC 
CTTCTGCTGT TCTGGGTGGG CCGCATGGTC ATGTTGGGGA CCCAGCTCAC AGGGCAGCTG 
CCCTTCAGCA AGGTGCTTCT TCATCCCATG GTTCGGGACA GGCAGGGCCG GAAGATGAGC 
AAGTCCCTGG GGAATGTGCT GGACCCAAGA GACATCATCA GTGGGGTGGA GATGCAGGTG 
CTGCAGGAAA AGCTGAGAAG CGGAAATTTG GACCCTGCAG AGCTGGCCAT TGTGGCTGCA 
GCACAGAAAA AGGACTTTCC TCACGGGATC CCTGAGTGTG GGACAGATGC CCTGAGATTC 
ACACTCTGCT CCCATGGAGT TCAGGTGGGC GACTTGCACC TGTCAGTCTC TGAGGTCCAG 
AGCTGCCGAC ATTTCTGCAA CAAGATCTGG AATGCTCTTC GCTTTATCCT CAATGCTTTA 
GGGGAGAAAT TTGTGCCACA GCCTGCTGAG GAGCTGTCTC CCTCCTCCCC GATGGATGCC 
TGGATCTTGA GCCGCCTTGC CCTGGCTGCC CAGGAGTGTG AGCGGGGCTT CCTCACCCGA 
GAGCTCTCGC TCGTCACTCA TGCCCTGCAC CACTTCTGGC TTCACAACCT CTGTGACGTC 
TACCTGGAGG CTGTGAAGCC CGTGCTGTGG CACTCGCCCC GCCCCCTGGG GCCCCCTCAG 
GTCCTGTTCT CCTGCGCTGA CCTCGGCCTC CGCCTCCTGG CCCCACTGAT GCCCTTCCTG 
GCTGAAGAGC TCTGGCAGAG GCTGCCCCCC AGGGCTGGTT GCCCCCCTGC CCCCAGCATC 
TCGGTTGCCC CCTACCCCAG CGCCTGCAGC TTGGAGCACT GGCGCCAGCC AGAGCTGGAG 
CGGCGCTTCT CCCGGGTCCA AGAGGTCGTG CAGGTGCTAA GGGCTCTCCG AGCCACGTAC 
CAGCTCACCA AAGCCCGGCC CCGAGTGCTG CTGCAGAGCT CAGAGCCTGG GGACCAGGGC 
CTCTTCGAGG CCTTCTTGGA GCCCCTGGGC ACCCTGGGCT ACTGTGGGGC TGTGGGCCTG 
TTACCCCCAG GCGCAGCAGC TCCCTCCGGC TGGGCCCAGG CTCCACTCAG TGACACGGCT 
CAAGTCTACA TGGAGCTGCA GGGCCTGGTG GACCCGCAGA TCCAGCTACC TCTGTTAGCC 
GCCCGAAGGT ACAAGTTGCA GAAGCAGCTT GATAGCCTCA CAGCCAGGAC CCCATCAGAA 
GGGGAGGCAG GGACTCAGAG GCAACAAAAG CTTTCTTCCC TCCAGCTGGA ATTGTCAAAA 
CTGGACAAGG CAGCCTCTCA CCTCCGGCAG CTGATGGATG AGCCTCCAGC CCCAGGGAGC 
CCGGAGCTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016812207.1
CDS	309..3500
Translation	MPHLPLASFRPPFWGLRHSRGLPRFHSVSTQSEPHGSPISRRNREAKQKRLREKQATLEAEIAGESKSPAESIKAWRPKELVLYEIPTKPGEKKDVSGPLPPAYSPRYVEAAWYPWWVREGFFKPEYQARLPQATGETFSMCIPPPNVTGSLHIGHALTVAIQDALVRWHRMRGDQVLWVPGSDHAGIATQAVVEKQLWKERGVRRHELSREAFLREVWQWKEAKGGEICEQLRALGASLDWDRECFTMDVGSSVAVTEAFVQLYKAGLLYRNHQLVNWSCALRSAISDIEVENRPLPGHTQLRLPGCPTPVSFGLLFSIAFPVDGEPDAEVVVGTTRPETLPGDVAVAVHPDDSRYTHLHGRQLRHPLMGQPLPLITDYAVQPHVGTGAVKVTPAHSPADAEMGARHGLSPLNVIAEDGTMTSLCGDWLQGLHRFVAREKIMSVLSERGLFRGLQNHPMVLPICSRSGDVIEYLLKNQWFVRCQEMGARAAKAVESGALELSPSFHQKNWQHWFSHIGDWCVSRQLWWGHQIPAYLVVEDHAQGEEDCWVVGRSEAEAREVAAELTGRPGAELTLERDPDVLDTWFSSALFPFSALGWPQETPDLARFYPLSLLETGSDLLLFWVGRMVMLGTQLTGQLPFSKVLLHPMVRDRQGRKMSKSLGNVLDPRDIISGVEMQVLQEKLRSGNLDPAELAIVAAAQKKDFPHGIPECGTDALRFTLCSHGVQVGDLHLSVSEVQSCRHFCNKIWNALRFILNALGEKFVPQPAEELSPSSPMDAWILSRLALAAQECERGFLTRELSLVTHALHHFWLHNLCDVYLEAVKPVLWHSPRPLGPPQVLFSCADLGLRLLAPLMPFLAEELWQRLPPRAGCPPAPSISVAPYPSACSLEHWRQPELERRFSRVQEVVQVLRALRATYQLTKARPRVLLQSSEPGDQGLFEAFLEPLGTLGYCGAVGLLPPGAAAPSGWAQAPLSDTAQVYMELQGLVDPQIQLPLLAARRYKLQKQLDSLTARTPSEGEAGTQRQQKLSSLQLELSKLDKAASHLRQLMDEPPAPGSPEL

Target ORF information:

RefSeq Version	XM_016956718.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes valyl-tRNA synthetase 2, mitochondrial (VARS2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016956718.1

ORF Insert Sequence:

ATGCCTCATT TGCCTCTCGC CTCTTTTCGA CCACCATTTT GGGGGCTGAG GCACTCACGG 
GGCCTCCCCA GGTTTCACTC CGTTTCTACA CAGTCGGAGC CCCATGGATC TCCCATCTCC 
CGGAGGAACC GTGAAGCCAA ACAGAAGCGC CTGCGAGAGA AGCAGGCGAC TCTGGAGGCT 
GAGATAGCAG GGGAGAGCAA GTCACCTGCA GAATCCATTA AGGCCTGGAG GCCTAAGGAG 
TTAGTATTGT ATGAAATCCC TACGAAACCC GGTGAAAAGA AAGATGTCTC TGGGCCCCTG 
CCTCCTGCAT ACAGCCCCCG ATATGTTGAG GCTGCCTGGT ACCCGTGGTG GGTACGAGAG 
GGCTTCTTCA AACCAGAATA TCAGGCCCGG CTGCCCCAAG CTACAGGGGA GACCTTTTCC 
ATGTGTATCC CACCTCCCAA TGTCACTGGC TCCCTGCACA TTGGCCACGC ACTCACGGTG 
GCCATACAGG ATGCCCTCGT GCGCTGGCAC CGGATGCGTG GGGATCAAGT GCTGTGGGTC 
CCTGGTTCAG ATCATGCAGG AATTGCTACA CAAGCTGTGG TGGAGAAACA ACTGTGGAAG 
GAACGGGGAG TGAGGAGACA TGAGCTGAGC CGGGAGGCCT TCCTTAGGGA GGTGTGGCAG 
TGGAAGGAGG CGAAAGGTGG AGAGATCTGT GAGCAGCTGC GAGCTCTGGG TGCCTCCCTG 
GACTGGGATC GAGAGTGTTT TACCATGGAT GTTGGCTCCT CAGTGGCTGT GACTGAAGCT 
TTTGTGCAGC TCTACAAGGC GGGGTTGCTG TACCGGAACC ATCAGCTTGT CAACTGGTCA 
TGTGCTTTAA GATCAGCCAT CTCAGACATT GAGGTGGAGA ACCGGCCCCT GCCTGGCCAC 
ACACAGCTTC GACTGCCTGG CTGCCCCACC CCCGTGTCTT TTGGCCTCCT ATTTTCTATT 
GCCTTCCCGG TGGATGGAGA GCCTGATGCA GAGGTTGTGG TAGGAACCAC AAGGCCAGAG 
ACGCTGCCTG GAGATGTGGC TGTGGCCGTT CATCCAGACG ACTCGCGATA CACACATCTA 
CACGGGCGAC AGCTTCGTCA CCCCTTGATG GGGCAGCCTC TTCCCCTCAT CACAGACTAT 
GCTGTTCAGC CACATGTGGG CACGGGGGCA GTGAAGGTGA CTCCAGCTCA CAGTCCTGCC 
GATGCTGAGA TGGGGGCCCG ACATGGCTTG AGCCCCTTGA ATGTCATTGC GGAGGATGGG 
ACCATGACCT CCCTCTGCGG GGACTGGCTG CAGGGTCTTC ACCGGTTTGT GGCCCGGGAA 
AAGATAATGT CTGTGCTGAG TGAACGGGGC CTGTTCCGGG GCCTCCAGAA CCACCCCATG 
GTACTGCCCA TCTGCAGCCG TTCTGGGGAT GTGATAGAAT ACCTGCTGAA GAACCAGTGG 
TTTGTCCGCT GCCAGGAAAT GGGGGCCCGA GCTGCCAAGG CTGTGGAGTC GGGGGCCCTG 
GAGCTCAGTC CCTCCTTCCA CCAGAAGAAT TGGCAGCACT GGTTTTCCCA TATTGGGGAC 
TGGTGTGTCT CCCGGCAGCT GTGGTGGGGC CATCAGATTC CAGCCTACCT GGTTGTAGAG 
GACCATGCGC AGGGAGAAGA GGACTGTTGG GTGGTTGGGC GGTCAGAGGC TGAGGCCAGA 
GAGGTAGCAG CGGAACTGAC AGGGAGGCCA GGGGCAGAGC TGACCCTGGA GAGGGATCCT 
GATGTCCTAG ACACATGGTT TTCTTCTGCC CTGTTCCCCT TTTCTGCCCT GGGCTGGCCC 
CAAGAGACCC CAGACCTTGC TCGTTTCTAC CCCCTGTCAC TTTTGGAAAC GGGCAGTGAC 
CTTCTGCTGT TCTGGGTGGG CCGCATGGTC ATGTTGGGGA CCCAGCTCAC AGGGCAGCTG 
CCCTTCAGCA AGGTGCTTCT TCATCCCATG GTTCGGGACA GGCAGGGCCG GAAGATGAGC 
AAGTCCCTGG GGAATGTGCT GGACCCAAGA GACATCATCA GTGGGGTGGA GATGCAGGTG 
CTGCAGGAAA AGCTGAGAAG CGGAAATTTG GACCCTGCAG AGCTGGCCAT TGTGGCTGCA 
GCACAGAAAA AGGACTTTCC TCACGGGATC CCTGAGTGTG GGACAGATGC CCTGAGATTC 
ACACTCTGCT CCCATGGAGT TCAGGTGGGC GACTTGCACC TGTCAGTCTC TGAGGTCCAG 
AGCTGCCGAC ATTTCTGCAA CAAGATCTGG AATGCTCTTC GCTTTATCCT CAATGCTTTA 
GGGGAGAAAT TTGTGCCACA GCCTGCTGAG GAGCTGTCTC CCTCCTCCCC GATGGATGCC 
TGGATCTTGA GCCGCCTTGC CCTGGCTGCC CAGGAGTGTG AGCGGGGCTT CCTCACCCGA 
GAGCTCTCGC TCGTCACTCA TGCCCTGCAC CACTTCTGGC TTCACAACCT CTGTGACGTC 
TACCTGGAGG CTGTGAAGCC CGTGCTGTGG CACTCGCCCC GCCCCCTGGG GCCCCCTCAG 
GTCCTGTTCT CCTGCGCTGA CCTCGGCCTC CGCCTCCTGG CCCCACTGAT GCCCTTCCTG 
GCTGAAGAGC TCTGGCAGAG GCTGCCCCCC AGGGCTGGTT GCCCCCCTGC CCCCAGCATC 
TCGGTTGCCC CCTACCCCAG CGCCTGCAGC TTGGAGCACT GGCGCCAGCC AGAGCTGGAG 
CGGCGCTTCT CCCGGGTCCA AGAGGTCGTG CAGGTGCTAA GGGCTCTCCG AGCCACGTAC 
CAGCTCACCA AAGCCCGGCC CCGAGTGCTG CTGCAGAGCT CAGAGCCTGG GGACCAGGGC 
CTCTTCGAGG CCTTCTTGGA GCCCCTGGGC ACCCTGGGCT ACTGTGGGGC TGTGGGCCTG 
TTACCCCCAG GCGCAGCAGC TCCCTCCGGC TGGGCCCAGG CTCCACTCAG TGACACGGCT 
CAAGTCTACA TGGAGCTGCA GGGCCTGGTG GACCCGCAGA TCCAGCTACC TCTGTTAGCC 
GCCCGAAGGT ACAAGTTGCA GAAGCAGCTT GATAGCCTCA CAGCCAGGAC CCCATCAGAA 
GGGGAGGCAG GGACTCAGAG GCAACAAAAG CTTTCTTCCC TCCAGCTGGA ATTGTCAAAA 
CTGGACAAGG CAGCCTCTCA CCTCCGGCAG CTGATGGATG AGCCTCCAGC CCCAGGGAGC 
CCGGAGCTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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VARS2 cDNA ORF clone, Pan troglodytes(chimpanzee)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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