BUB1 cDNA ORF clone, Ovis aries(sheep)

Gene
Clones
gRNAs

Summary

Gene Symbol	BUB1
Entrez Gene ID	101117404
Full Name	BUB1 mitotic checkpoint serine/threonine kinase
Gene Type	protein-coding
Organism	Ovis aries(sheep)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012173898.2	XP_012029288.1	mitotic checkpoint serine/threonine-protein kinase BUB1 isoform X2
XM_012150065.2	XP_012005455.1	mitotic checkpoint serine/threonine-protein kinase BUB1 isoform X3
XM_012150067.2	XP_012005457.1	mitotic checkpoint serine/threonine-protein kinase BUB1 isoform X1
XM_012150069.2	XP_012005459.1	mitotic checkpoint serine/threonine-protein kinase BUB1 isoform X2
XM_012173898.3	XP_012029288.2	mitotic checkpoint serine/threonine-protein kinase BUB1
XM_015109180.1	XP_014964666.1	mitotic checkpoint serine/threonine-protein kinase BUB1 isoform X4
XM_012150066.2	XP_012005456.1	mitotic checkpoint serine/threonine-protein kinase BUB1 isoform X5
XM_004006184.3	XP_004006233.1	mitotic checkpoint serine/threonine-protein kinase BUB1 isoform X1

Pathways from BioSystems
Gene Ontology

The following BUB1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the BUB1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OOv26086	XM_012150065.2 Latest version!	Ovis aries musimon BUB1, mitotic checkpoint serine/threonine kinase (BUB1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OOv26087	XM_015109180.1 Latest version!	Ovis aries musimon BUB1, mitotic checkpoint serine/threonine kinase (BUB1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OOv26088	XM_012150066.2 Latest version!	Ovis aries musimon BUB1, mitotic checkpoint serine/threonine kinase (BUB1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OOv26089	XM_012150067.2 Latest version!	Ovis aries musimon BUB1, mitotic checkpoint serine/threonine kinase (BUB1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OOv26090	XM_012150069.2 Latest version!	Ovis aries musimon BUB1, mitotic checkpoint serine/threonine kinase (BUB1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OSm77604	XM_012173898.3 Latest version!	Ovis aries BUB1 mitotic checkpoint serine/threonine kinase (BUB1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OSm04132	XM_004006184.3 Latest version!	Ovis aries BUB1, mitotic checkpoint serine/threonine kinase (BUB1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OSm38428	XM_012173898.2 Latest version!	Ovis aries BUB1, mitotic checkpoint serine/threonine kinase (BUB1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

BUB1 ( XM_012150065.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_012005455.1 Ovis aries musimon BUB1, mitotic checkpoint serine/threonine kinase (BUB1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OOv26086
Clone ID Related Accession (Same CDS sequence)	XM_012150065.2
Accession Version	XM_012150065.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3411bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	mitotic checkpoint serine/threonine-protein kinase BUB1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942623.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012150065.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGAGGTG CCCTCGGGGA CGCGTACCTG GGCCGGAAGA GCGGACAGCC GCCCAGGAGC 
CCGCGCAGCT CGTTTTTGAG GCTCCAGAAC TCGCCGTCCA GAAACGCGGA ACAAAAAACT 
GCGGGAAATG GAGAGGCGTG CCACTCGGCA GGATTCCGTT TTTCACCCCA TCAGCCTCTC 
CTAAGCCAGA GAATGTTTGA AGCCCATATG CAGAGCTACA AGGGCGATGA CCCACTTGGT 
GAATGGGAAA GTTACATGCG ATGGGTAGAA GAGAATTTTC CTGAGAATAA AGAATACTTA 
ACAACTTTAT TAGAGCATCT AATGAAGGAA TTTTTAGATA AGAAGAGATA TCACAATGAC 
CCAAGGTTCA TCAATTATTG TCTACAATTT GCCAAGTGCA ACAGTGACCT TCACCAGTTT 
TTTGAGTTTC TGCACAACCA CGGTATCGGA ACCCAGGCAG CTCCTCTGTA CGTAGCCTGG 
GCCGGGCACC TGGAAGGCCA GGGGGAGTGG CAGCACGCCA GTGCCGTTTT TCGGAGAGGA 
ATTCAGAACC AGGCCGAACC TAGAGAGCTG CTGCAACAAC AGTACAGGAT GTTTCAGACA 
CGCCTCACTG AAACCCATTT GTCTACTCAA GTGAGAACCT CAGAACCTCT GCATGATGCT 
CAGATTTTAA ATCAAATGGT AACATCAAAC TCAAATCCAG GAAGTAACTC AGCCTGCATT 
TCTAAGAATC AGGGTCCACA ACTTCCTGGA GCAACACCTG CAGCTGGTGA TAAAGGGTCA 
GACATGGAAC AAAGGGTGAT CACAATTTCT AAATCAGAAT ATTCTTCACA CGCATCTGTG 
GCAGACAAAG CTGATGTTCA GCAGGTTGTC ATGTATTGCA AGGAGAAGCT TATTCGCGGA 
GAATCGGAGT TTTCCTTCGA AGAGCTGAGA GCCCAGAGAT ACAATCTACG GAGAAAGCAT 
GAACAATGGG TAAATGAAGA GAGGCATTAT ATGAAAAGGA AAGAAGCAAA TGCTTTGGAA 
GAACAACTAT TAAAACAGAA AATGGATGAG CTTCATAAGA AGCTGCACCA GGTGGTGGAG 
ATGTCGCAGG GGAACCTGCC CTCTTCCCGG GGGACGTCTG AGGTCCGTCC AGCGTGTTTG 
GAGGCACACA CAGACTCCCA GCAGGAACTA AGAGCTCCGG GTCTTCCAGC CGTCCCTCAG 
CAGACCTCGA AGAACGCGGA CGAGAAGCCC GGGGAGGCAC CTTCTGTCCC TCTTACAGCA 
AGTGCTGTCG CCACTGCTTT GGTGTCCCCG GCCGCCAGCC AGGGTGTGGC TTGCCCGGCT 
CCTTTGGCAG ACCCGCCCGT GACGGACGCC GTGTTTTCAG GGGGCGGCAG AGATGCCAGG 
TGTGTGAACA AAAGTACTCA TGAATTCAGG CCACAGAGCG GAGCAGAGAT AAAAGAAGGG 
TGTGAAACAC AGAAGGTTGT TAACACAAGT TCCTTTCACA CAACTCCAAA CACATCTCTG 
GGACTGGTTC AGGCAACACC ATCCAAAGTG CAGCCATCAC CCACGGTGCA CACCAAGGAA 
GCTCTCGGTT TCATCATGAA TGTGTTTCAG GCTCCTACAC TTCCTGATAT TTCCGATGAC 
AAAGAGGAAT GGCCATCTCT AGGTCAAAAC GAGTTTGAAG CCCAGTTTCA AAAAAATGCA 
CCATCTTCTG GGGCTTGGGG AGTCAATAAG ATCATCTCTT CTTTGTCATC TGCTTTTCCT 
GTATTTGAAG ATGGAAACAA AGAAAATTAT GGATTACCAC AGCCGAAAAA TAAACCTGCA 
GGAGCCAGGA CCTTTGGAGA ACGTTCCGTG AGCAGGTTTC CTGCAAAACC AAATGAAGTG 
CCTCATGCTG ACGAGTTTCT GGATGATTCA ACCGTGTGGG GTGTCCGCTG CAACAAAAGC 
CTGGCACCCA GCCCCAGGAG CCCGGGAGAC TTCAGGTCCG CCGCGCAGCT GGCGTCTACG 
CCCTTCCACA GGCTTCCCGC GGACTTCGCG CACCCACTGG AAGATAAAGA AAATATGGTG 
GCGAACCAGC ACACCCACGT GGCTGTGGAT CCTTGTGCAA GAAACAAGGT GGAACCCTTG 
AAAGAGAGAA GGTTCAGTCC AGTCGAAGAG ACAAGCCCAG ACCCAGTGTG CCCCACAGAG 
GGCCCTTCGG CGTCCTTGCT CCGTCTGGGC CAGCCTGCTG CAGCCGTACT GGTGAGCGAG 
GCAGAGCAGG GTCTCAAGGC CTGTAAAGTC ACAGACCCCG ACTTGGCCCC TGCGGAGGAT 
CCGGCAGAAG CCAGCGCTGG GCTCCAGGCT GAACTGATGC AGGCTGGCCC GCCTGGGAAT 
GTCAACGCTC CAAACTTCAC TGTTGAGAAC CCCTGGGATG AGGAGTTGAT TCTCAAACTT 
TTATCCGGGC TTTCTAAACC AGTGAGTTCC TATCCAAATA CTTATGAATG GGCATGTAAG 
CTTCCAGCCA TCAAGCCCAA GACTGAATTC CAGTTAGGTT CTTTGCTGGT CTACGTTGAT 
CACTTTATTG GAGAAGGGGC TTTCGCCCAA GTCTATGAAG TTACCCATGG AGATGTGAAC 
AACAGCAAAA ATAAGGAGAA ATTTGCTTTG AAGGTCCAGA AGCCTCCCAA CCTCTGGGAG 
TTCTACATTG GGACCCAGCT GATGGAAAGA CTGAACCCAG GTGCGCAGCA CATGTTCATC 
AGGTTCTATT CGGCCCACCT GTTCCAGAAC GGCAGCGTGT TGGTGGGGGA CCTCTACAGC 
TACGGCACGC TGTTGAATGC CATTAATCTC TATAAAAATA CCCCTGAAAA AGTGATGCCT 
CAGGCACTAG TCCTCGCTTT TGCCATCAGA ATGCTCTGCA TGATCCAGCA AGTCCATGAC 
TGTGAAATCA TCCACGGAGA CATCAAGCCA GACAATTTCA TACTCGGAAA CAGATTTTTG 
GAACAAGACA ACAAAGATGA TGACTTATCT GCCGGCTTGG TCCTGATTGA CCTGGGTCAG 
AGTATAGACA TGAAACTTTT TCCGAAAGGA ACTGCATTTA CAGGGAAGTG TGAAACTTCT 
GGTTTCCAGT GTATTGAGAT GCTCAGTAAC AAGCCATGGA ACTACCAGAT TGATTACTTT 
GGAGTTGCTG CAACCATATA CTGCATGCTT TGTGGCACTT ACATGAAAGT GAAAAATGAA 
GGGGGCATCT GGAAGCCTGA AGGTCTTTTT AGAAGGCTTC CTCACCTGGA CATGTGGAAT 
GAATTTTTTC ACGTAATGTT GAATATACCC GATTGTCATC ATCTTCCATC TTTGGATTTG 
TTAAGGCAGA AGCTAAAGAA AACATTTCAA GAACACTACA CCAGCAAGAT CAAGACCCTG 
TGTAATAGGC TAATTGTATT GCTCTTAGAA TATAAGCGTT CTCGGAAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012005455.1
CDS	3330..6740
Translation	MRGALGDAYLGRKSGQPPRSPRSSFLRLQNSPSRNAEQKTAGNGEACHSAGFRFSPHQPLLSQRMFEAHMQSYKGDDPLGEWESYMRWVEENFPENKEYLTTLLEHLMKEFLDKKRYHNDPRFINYCLQFAKCNSDLHQFFEFLHNHGIGTQAAPLYVAWAGHLEGQGEWQHASAVFRRGIQNQAEPRELLQQQYRMFQTRLTETHLSTQVRTSEPLHDAQILNQMVTSNSNPGSNSACISKNQGPQLPGATPAAGDKGSDMEQRVITISKSEYSSHASVADKADVQQVVMYCKEKLIRGESEFSFEELRAQRYNLRRKHEQWVNEERHYMKRKEANALEEQLLKQKMDELHKKLHQVVEMSQGNLPSSRGTSEVRPACLEAHTDSQQELRAPGLPAVPQQTSKNADEKPGEAPSVPLTASAVATALVSPAASQGVACPAPLADPPVTDAVFSGGGRDARCVNKSTHEFRPQSGAEIKEGCETQKVVNTSSFHTTPNTSLGLVQATPSKVQPSPTVHTKEALGFIMNVFQAPTLPDISDDKEEWPSLGQNEFEAQFQKNAPSSGAWGVNKIISSLSSAFPVFEDGNKENYGLPQPKNKPAGARTFGERSVSRFPAKPNEVPHADEFLDDSTVWGVRCNKSLAPSPRSPGDFRSAAQLASTPFHRLPADFAHPLEDKENMVANQHTHVAVDPCARNKVEPLKERRFSPVEETSPDPVCPTEGPSASLLRLGQPAAAVLVSEAEQGLKACKVTDPDLAPAEDPAEASAGLQAELMQAGPPGNVNAPNFTVENPWDEELILKLLSGLSKPVSSYPNTYEWACKLPAIKPKTEFQLGSLLVYVDHFIGEGAFAQVYEVTHGDVNNSKNKEKFALKVQKPPNLWEFYIGTQLMERLNPGAQHMFIRFYSAHLFQNGSVLVGDLYSYGTLLNAINLYKNTPEKVMPQALVLAFAIRMLCMIQQVHDCEIIHGDIKPDNFILGNRFLEQDNKDDDLSAGLVLIDLGQSIDMKLFPKGTAFTGKCETSGFQCIEMLSNKPWNYQIDYFGVAATIYCMLCGTYMKVKNEGGIWKPEGLFRRLPHLDMWNEFFHVMLNIPDCHHLPSLDLLRQKLKKTFQEHYTSKIKTLCNRLIVLLLEYKRSRK

Target ORF information:

RefSeq Version	XM_012150065.2
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon BUB1, mitotic checkpoint serine/threonine kinase (BUB1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012150065.2

ORF Insert Sequence:

ATGCGAGGTG CCCTCGGGGA CGCGTACCTG GGCCGGAAGA GCGGACAGCC GCCCAGGAGC 
CCGCGCAGCT CGTTTTTGAG GCTCCAGAAC TCGCCGTCCA GAAACGCGGA ACAAAAAACT 
GCGGGAAATG GAGAGGCGTG CCACTCGGCA GGATTCCGTT TTTCACCCCA TCAGCCTCTC 
CTAAGCCAGA GAATGTTTGA AGCCCATATG CAGAGCTACA AGGGCGATGA CCCACTTGGT 
GAATGGGAAA GTTACATGCG ATGGGTAGAA GAGAATTTTC CTGAGAATAA AGAATACTTA 
ACAACTTTAT TAGAGCATCT AATGAAGGAA TTTTTAGATA AGAAGAGATA TCACAATGAC 
CCAAGGTTCA TCAATTATTG TCTACAATTT GCCAAGTGCA ACAGTGACCT TCACCAGTTT 
TTTGAGTTTC TGCACAACCA CGGTATCGGA ACCCAGGCAG CTCCTCTGTA CGTAGCCTGG 
GCCGGGCACC TGGAAGGCCA GGGGGAGTGG CAGCACGCCA GTGCCGTTTT TCGGAGAGGA 
ATTCAGAACC AGGCCGAACC TAGAGAGCTG CTGCAACAAC AGTACAGGAT GTTTCAGACA 
CGCCTCACTG AAACCCATTT GTCTACTCAA GTGAGAACCT CAGAACCTCT GCATGATGCT 
CAGATTTTAA ATCAAATGGT AACATCAAAC TCAAATCCAG GAAGTAACTC AGCCTGCATT 
TCTAAGAATC AGGGTCCACA ACTTCCTGGA GCAACACCTG CAGCTGGTGA TAAAGGGTCA 
GACATGGAAC AAAGGGTGAT CACAATTTCT AAATCAGAAT ATTCTTCACA CGCATCTGTG 
GCAGACAAAG CTGATGTTCA GCAGGTTGTC ATGTATTGCA AGGAGAAGCT TATTCGCGGA 
GAATCGGAGT TTTCCTTCGA AGAGCTGAGA GCCCAGAGAT ACAATCTACG GAGAAAGCAT 
GAACAATGGG TAAATGAAGA GAGGCATTAT ATGAAAAGGA AAGAAGCAAA TGCTTTGGAA 
GAACAACTAT TAAAACAGAA AATGGATGAG CTTCATAAGA AGCTGCACCA GGTGGTGGAG 
ATGTCGCAGG GGAACCTGCC CTCTTCCCGG GGGACGTCTG AGGTCCGTCC AGCGTGTTTG 
GAGGCACACA CAGACTCCCA GCAGGAACTA AGAGCTCCGG GTCTTCCAGC CGTCCCTCAG 
CAGACCTCGA AGAACGCGGA CGAGAAGCCC GGGGAGGCAC CTTCTGTCCC TCTTACAGCA 
AGTGCTGTCG CCACTGCTTT GGTGTCCCCG GCCGCCAGCC AGGGTGTGGC TTGCCCGGCT 
CCTTTGGCAG ACCCGCCCGT GACGGACGCC GTGTTTTCAG GGGGCGGCAG AGATGCCAGG 
TGTGTGAACA AAAGTACTCA TGAATTCAGG CCACAGAGCG GAGCAGAGAT AAAAGAAGGG 
TGTGAAACAC AGAAGGTTGT TAACACAAGT TCCTTTCACA CAACTCCAAA CACATCTCTG 
GGACTGGTTC AGGCAACACC ATCCAAAGTG CAGCCATCAC CCACGGTGCA CACCAAGGAA 
GCTCTCGGTT TCATCATGAA TGTGTTTCAG GCTCCTACAC TTCCTGATAT TTCCGATGAC 
AAAGAGGAAT GGCCATCTCT AGGTCAAAAC GAGTTTGAAG CCCAGTTTCA AAAAAATGCA 
CCATCTTCTG GGGCTTGGGG AGTCAATAAG ATCATCTCTT CTTTGTCATC TGCTTTTCCT 
GTATTTGAAG ATGGAAACAA AGAAAATTAT GGATTACCAC AGCCGAAAAA TAAACCTGCA 
GGAGCCAGGA CCTTTGGAGA ACGTTCCGTG AGCAGGTTTC CTGCAAAACC AAATGAAGTG 
CCTCATGCTG ACGAGTTTCT GGATGATTCA ACCGTGTGGG GTGTCCGCTG CAACAAAAGC 
CTGGCACCCA GCCCCAGGAG CCCGGGAGAC TTCAGGTCCG CCGCGCAGCT GGCGTCTACG 
CCCTTCCACA GGCTTCCCGC GGACTTCGCG CACCCACTGG AAGATAAAGA AAATATGGTG 
GCGAACCAGC ACACCCACGT GGCTGTGGAT CCTTGTGCAA GAAACAAGGT GGAACCCTTG 
AAAGAGAGAA GGTTCAGTCC AGTCGAAGAG ACAAGCCCAG ACCCAGTGTG CCCCACAGAG 
GGCCCTTCGG CGTCCTTGCT CCGTCTGGGC CAGCCTGCTG CAGCCGTACT GGTGAGCGAG 
GCAGAGCAGG GTCTCAAGGC CTGTAAAGTC ACAGACCCCG ACTTGGCCCC TGCGGAGGAT 
CCGGCAGAAG CCAGCGCTGG GCTCCAGGCT GAACTGATGC AGGCTGGCCC GCCTGGGAAT 
GTCAACGCTC CAAACTTCAC TGTTGAGAAC CCCTGGGATG AGGAGTTGAT TCTCAAACTT 
TTATCCGGGC TTTCTAAACC AGTGAGTTCC TATCCAAATA CTTATGAATG GGCATGTAAG 
CTTCCAGCCA TCAAGCCCAA GACTGAATTC CAGTTAGGTT CTTTGCTGGT CTACGTTGAT 
CACTTTATTG GAGAAGGGGC TTTCGCCCAA GTCTATGAAG TTACCCATGG AGATGTGAAC 
AACAGCAAAA ATAAGGAGAA ATTTGCTTTG AAGGTCCAGA AGCCTCCCAA CCTCTGGGAG 
TTCTACATTG GGACCCAGCT GATGGAAAGA CTGAACCCAG GTGCGCAGCA CATGTTCATC 
AGGTTCTATT CGGCCCACCT GTTCCAGAAC GGCAGCGTGT TGGTGGGGGA CCTCTACAGC 
TACGGCACGC TGTTGAATGC CATTAATCTC TATAAAAATA CCCCTGAAAA AGTGATGCCT 
CAGGCACTAG TCCTCGCTTT TGCCATCAGA ATGCTCTGCA TGATCCAGCA AGTCCATGAC 
TGTGAAATCA TCCACGGAGA CATCAAGCCA GACAATTTCA TACTCGGAAA CAGATTTTTG 
GAACAAGACA ACAAAGATGA TGACTTATCT GCCGGCTTGG TCCTGATTGA CCTGGGTCAG 
AGTATAGACA TGAAACTTTT TCCGAAAGGA ACTGCATTTA CAGGGAAGTG TGAAACTTCT 
GGTTTCCAGT GTATTGAGAT GCTCAGTAAC AAGCCATGGA ACTACCAGAT TGATTACTTT 
GGAGTTGCTG CAACCATATA CTGCATGCTT TGTGGCACTT ACATGAAAGT GAAAAATGAA 
GGGGGCATCT GGAAGCCTGA AGGTCTTTTT AGAAGGCTTC CTCACCTGGA CATGTGGAAT 
GAATTTTTTC ACGTAATGTT GAATATACCC GATTGTCATC ATCTTCCATC TTTGGATTTG 
TTAAGGCAGA AGCTAAAGAA AACATTTCAA GAACACTACA CCAGCAAGAT CAAGACCCTG 
TGTAATAGGC TAATTGTATT GCTCTTAGAA TATAAGCGTT CTCGGAAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BUB1 ( XM_015109180.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_014964666.1 Ovis aries musimon BUB1, mitotic checkpoint serine/threonine kinase (BUB1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OOv26087
Clone ID Related Accession (Same CDS sequence)	XM_015109180.1
Accession Version	XM_015109180.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3402bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	mitotic checkpoint serine/threonine-protein kinase BUB1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942623.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGAGGTG CCCTCGGGGA CGCGTACCTG GGCCGGAAGA GCGGACAGCC GCCCAGGAGC 
CCGCGCAGCT CGTTTTTGAG GCTCCAGAAC TCGCCGTCCA GAAACGCGGA ACAAAAAACT 
GCGGGAAATG GAGAGGCGTG CCACTCGGCA GGATTCCGTT TTTCACCCCA TCAGCCTCTC 
CTAAGCCAGA GAATGTTTGA AGCCCATATG CAGAGCTACA AGGGCGATGA CCCACTTGGT 
GAATGGGAAA GTTACATGCG ATGGGTAGAA GAGAATTTTC CTGAGAATAA AGAATACTTA 
ACAACTTTAT TAGAGCATCT AATGAAGGAA TTTTTAGATA AGAAGAGATA TCACAATGAC 
CCAAGGTTCA TCAATTATTG TCTACAATTT GCCAAGTGCA ACAGTGACCT TCACCAGTTT 
TTTGAGTTTC TGCACAACCA CGGTATCGGA ACCCAGGCAG CTCCTCTGTA CGTAGCCTGG 
GCCGGGCACC TGGAAGGCCA GGGGGAGTGG CAGCACGCCA GTGCCGTTTT TCGGAGAGGA 
ATTCAGAACC AGGCCGAACC TAGAGAGCTG CTGCAACAAC AGTACAGGAT GTTTCAGACA 
CGCCTCACTG AAACCCATTT GTCTACTCAA GTGAGAACCT CAGAACCTCT GCATGATGCT 
CAGATTTTAA ATCAAATGGT AACATCAAAC TCAAATCCAG GAAGTAACTC AGCCTGCATT 
TCTAAGAATC AGGGTCCACA ACTTCCTGGA GCAACACCTG CAGCTGGTGA TAAAGGGGAA 
CAAAGGGTGA TCACAATTTC TAAATCAGAA TATTCTTCAC ACGCATCTGT GGCAGACAAA 
GCTGATGTTC AGCAGGTTGT CATGTATTGC AAGGAGAAGC TTATTCGCGG AGAATCGGAG 
TTTTCCTTCG AAGAGCTGAG AGCCCAGAGA TACAATCTAC GGAGAAAGCA TGAACAATGG 
GTAAATGAAG AGAGGCATTA TATGAAAAGG AAAGAAGCAA ATGCTTTGGA AGAACAACTA 
TTAAAACAGA AAATGGATGA GCTTCATAAG AAGCTGCACC AGGTGGTGGA GATGTCGCAG 
GGGAACCTGC CCTCTTCCCG GGGGACGTCT GAGGTCCGTC CAGCGTGTTT GGAGGCACAC 
ACAGACTCCC AGCAGGAACT AAGAGCTCCG GGTCTTCCAG CCGTCCCTCA GCAGACCTCG 
AAGAACGCGG ACGAGAAGCC CGGGGAGGCA CCTTCTGTCC CTCTTACAGC AAGTGCTGTC 
GCCACTGCTT TGGTGTCCCC GGCCGCCAGC CAGGGTGTGG CTTGCCCGGC TCCTTTGGCA 
GACCCGCCCG TGACGGACGC CGTGTTTTCA GGGGGCGGCA GAGATGCCAG GTGTGTGAAC 
AAAAGTACTC ATGAATTCAG GCCACAGAGC GGAGCAGAGA TAAAAGAAGG GTGTGAAACA 
CAGAAGGTTG TTAACACAAG TTCCTTTCAC ACAACTCCAA ACACATCTCT GGGACTGGTT 
CAGGCAACAC CATCCAAAGT GCAGCCATCA CCCACGGTGC ACACCAAGGA AGCTCTCGGT 
TTCATCATGA ATGTGTTTCA GGCTCCTACA CTTCCTGATA TTTCCGATGA CAAAGAGGAA 
TGGCCATCTC TAGGTCAAAA CGAGTTTGAA GCCCAGTTTC AAAAAAATGC ACCATCTTCT 
GGGGCTTGGG GAGTCAATAA GATCATCTCT TCTTTGTCAT CTGCTTTTCC TGTATTTGAA 
GATGGAAACA AAGAAAATTA TGGATTACCA CAGCCGAAAA ATAAACCTGC AGGAGCCAGG 
ACCTTTGGAG AACGTTCCGT GAGCAGGTTT CCTGCAAAAC CAAATGAAGT GCCTCATGCT 
GACGAGTTTC TGGATGATTC AACCGTGTGG GGTGTCCGCT GCAACAAAAG CCTGGCACCC 
AGCCCCAGGA GCCCGGGAGA CTTCAGGTCC GCCGCGCAGC TGGCGTCTAC GCCCTTCCAC 
AGGCTTCCCG CGGACTTCGC GCACCCACTG GAAGATAAAG AAAATATGGT GGCGAACCAG 
CACACCCACG TGGCTGTGGA TCCTTGTGCA AGAAACAAGG TGGAACCCTT GAAAGAGAGA 
AGGTTCAGTC CAGTCGAAGA GACAAGCCCA GACCCAGTGT GCCCCACAGA GGGCCCTTCG 
GCGTCCTTGC TCCGTCTGGG CCAGCCTGCT GCAGCCGTAC TGGTGAGCGA GGCAGAGCAG 
GGTCTCAAGG CCTGTAAAGT CACAGACCCC GACTTGGCCC CTGCGGAGGA TCCGGCAGAA 
GCCAGCGCTG GGCTCCAGGC TGAACTGATG CAGGCTGGCC CGCCTGGGAA TGTCAACGCT 
CCAAACTTCA CTGTTGAGAA CCCCTGGGAT GAGGAGTTGA TTCTCAAACT TTTATCCGGG 
CTTTCTAAAC CAGTGAGTTC CTATCCAAAT ACTTATGAAT GGGCATGTAA GCTTCCAGCC 
ATCAAGCCCA AGACTGAATT CCAGTTAGGT TCTTTGCTGG TCTACGTTGA TCACTTTATT 
GGAGAAGGGG CTTTCGCCCA AGTCTATGAA GTTACCCATG GAGATGTGAA CAACAGCAAA 
AATAAGGAGA AATTTGCTTT GAAGGTCCAG AAGCCTCCCA ACCTCTGGGA GTTCTACATT 
GGGACCCAGC TGATGGAAAG ACTGAACCCA GGTGCGCAGC ACATGTTCAT CAGGTTCTAT 
TCGGCCCACC TGTTCCAGAA CGGCAGCGTG TTGGTGGGGG ACCTCTACAG CTACGGCACG 
CTGTTGAATG CCATTAATCT CTATAAAAAT ACCCCTGAAA AAGTGATGCC TCAGGCACTA 
GTCCTCGCTT TTGCCATCAG AATGCTCTGC ATGATCCAGC AAGTCCATGA CTGTGAAATC 
ATCCACGGAG ACATCAAGCC AGACAATTTC ATACTCGGAA ACAGATTTTT GGAACAAGAC 
AACAAAGATG ATGACTTATC TGCCGGCTTG GTCCTGATTG ACCTGGGTCA GAGTATAGAC 
ATGAAACTTT TTCCGAAAGG AACTGCATTT ACAGGGAAGT GTGAAACTTC TGGTTTCCAG 
TGTATTGAGA TGCTCAGTAA CAAGCCATGG AACTACCAGA TTGATTACTT TGGAGTTGCT 
GCAACCATAT ACTGCATGCT TTGTGGCACT TACATGAAAG TGAAAAATGA AGGGGGCATC 
TGGAAGCCTG AAGGTCTTTT TAGAAGGCTT CCTCACCTGG ACATGTGGAA TGAATTTTTT 
CACGTAATGT TGAATATACC CGATTGTCAT CATCTTCCAT CTTTGGATTT GTTAAGGCAG 
AAGCTAAAGA AAACATTTCA AGAACACTAC ACCAGCAAGA TCAAGACCCT GTGTAATAGG 
CTAATTGTAT TGCTCTTAGA ATATAAGCGT TCTCGGAAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014964666.1
CDS	3330..6731
Translation	MRGALGDAYLGRKSGQPPRSPRSSFLRLQNSPSRNAEQKTAGNGEACHSAGFRFSPHQPLLSQRMFEAHMQSYKGDDPLGEWESYMRWVEENFPENKEYLTTLLEHLMKEFLDKKRYHNDPRFINYCLQFAKCNSDLHQFFEFLHNHGIGTQAAPLYVAWAGHLEGQGEWQHASAVFRRGIQNQAEPRELLQQQYRMFQTRLTETHLSTQVRTSEPLHDAQILNQMVTSNSNPGSNSACISKNQGPQLPGATPAAGDKGEQRVITISKSEYSSHASVADKADVQQVVMYCKEKLIRGESEFSFEELRAQRYNLRRKHEQWVNEERHYMKRKEANALEEQLLKQKMDELHKKLHQVVEMSQGNLPSSRGTSEVRPACLEAHTDSQQELRAPGLPAVPQQTSKNADEKPGEAPSVPLTASAVATALVSPAASQGVACPAPLADPPVTDAVFSGGGRDARCVNKSTHEFRPQSGAEIKEGCETQKVVNTSSFHTTPNTSLGLVQATPSKVQPSPTVHTKEALGFIMNVFQAPTLPDISDDKEEWPSLGQNEFEAQFQKNAPSSGAWGVNKIISSLSSAFPVFEDGNKENYGLPQPKNKPAGARTFGERSVSRFPAKPNEVPHADEFLDDSTVWGVRCNKSLAPSPRSPGDFRSAAQLASTPFHRLPADFAHPLEDKENMVANQHTHVAVDPCARNKVEPLKERRFSPVEETSPDPVCPTEGPSASLLRLGQPAAAVLVSEAEQGLKACKVTDPDLAPAEDPAEASAGLQAELMQAGPPGNVNAPNFTVENPWDEELILKLLSGLSKPVSSYPNTYEWACKLPAIKPKTEFQLGSLLVYVDHFIGEGAFAQVYEVTHGDVNNSKNKEKFALKVQKPPNLWEFYIGTQLMERLNPGAQHMFIRFYSAHLFQNGSVLVGDLYSYGTLLNAINLYKNTPEKVMPQALVLAFAIRMLCMIQQVHDCEIIHGDIKPDNFILGNRFLEQDNKDDDLSAGLVLIDLGQSIDMKLFPKGTAFTGKCETSGFQCIEMLSNKPWNYQIDYFGVAATIYCMLCGTYMKVKNEGGIWKPEGLFRRLPHLDMWNEFFHVMLNIPDCHHLPSLDLLRQKLKKTFQEHYTSKIKTLCNRLIVLLLEYKRSRK

Target ORF information:

RefSeq Version	XM_015109180.1
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon BUB1, mitotic checkpoint serine/threonine kinase (BUB1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015109180.1

ORF Insert Sequence:

ATGCGAGGTG CCCTCGGGGA CGCGTACCTG GGCCGGAAGA GCGGACAGCC GCCCAGGAGC 
CCGCGCAGCT CGTTTTTGAG GCTCCAGAAC TCGCCGTCCA GAAACGCGGA ACAAAAAACT 
GCGGGAAATG GAGAGGCGTG CCACTCGGCA GGATTCCGTT TTTCACCCCA TCAGCCTCTC 
CTAAGCCAGA GAATGTTTGA AGCCCATATG CAGAGCTACA AGGGCGATGA CCCACTTGGT 
GAATGGGAAA GTTACATGCG ATGGGTAGAA GAGAATTTTC CTGAGAATAA AGAATACTTA 
ACAACTTTAT TAGAGCATCT AATGAAGGAA TTTTTAGATA AGAAGAGATA TCACAATGAC 
CCAAGGTTCA TCAATTATTG TCTACAATTT GCCAAGTGCA ACAGTGACCT TCACCAGTTT 
TTTGAGTTTC TGCACAACCA CGGTATCGGA ACCCAGGCAG CTCCTCTGTA CGTAGCCTGG 
GCCGGGCACC TGGAAGGCCA GGGGGAGTGG CAGCACGCCA GTGCCGTTTT TCGGAGAGGA 
ATTCAGAACC AGGCCGAACC TAGAGAGCTG CTGCAACAAC AGTACAGGAT GTTTCAGACA 
CGCCTCACTG AAACCCATTT GTCTACTCAA GTGAGAACCT CAGAACCTCT GCATGATGCT 
CAGATTTTAA ATCAAATGGT AACATCAAAC TCAAATCCAG GAAGTAACTC AGCCTGCATT 
TCTAAGAATC AGGGTCCACA ACTTCCTGGA GCAACACCTG CAGCTGGTGA TAAAGGGGAA 
CAAAGGGTGA TCACAATTTC TAAATCAGAA TATTCTTCAC ACGCATCTGT GGCAGACAAA 
GCTGATGTTC AGCAGGTTGT CATGTATTGC AAGGAGAAGC TTATTCGCGG AGAATCGGAG 
TTTTCCTTCG AAGAGCTGAG AGCCCAGAGA TACAATCTAC GGAGAAAGCA TGAACAATGG 
GTAAATGAAG AGAGGCATTA TATGAAAAGG AAAGAAGCAA ATGCTTTGGA AGAACAACTA 
TTAAAACAGA AAATGGATGA GCTTCATAAG AAGCTGCACC AGGTGGTGGA GATGTCGCAG 
GGGAACCTGC CCTCTTCCCG GGGGACGTCT GAGGTCCGTC CAGCGTGTTT GGAGGCACAC 
ACAGACTCCC AGCAGGAACT AAGAGCTCCG GGTCTTCCAG CCGTCCCTCA GCAGACCTCG 
AAGAACGCGG ACGAGAAGCC CGGGGAGGCA CCTTCTGTCC CTCTTACAGC AAGTGCTGTC 
GCCACTGCTT TGGTGTCCCC GGCCGCCAGC CAGGGTGTGG CTTGCCCGGC TCCTTTGGCA 
GACCCGCCCG TGACGGACGC CGTGTTTTCA GGGGGCGGCA GAGATGCCAG GTGTGTGAAC 
AAAAGTACTC ATGAATTCAG GCCACAGAGC GGAGCAGAGA TAAAAGAAGG GTGTGAAACA 
CAGAAGGTTG TTAACACAAG TTCCTTTCAC ACAACTCCAA ACACATCTCT GGGACTGGTT 
CAGGCAACAC CATCCAAAGT GCAGCCATCA CCCACGGTGC ACACCAAGGA AGCTCTCGGT 
TTCATCATGA ATGTGTTTCA GGCTCCTACA CTTCCTGATA TTTCCGATGA CAAAGAGGAA 
TGGCCATCTC TAGGTCAAAA CGAGTTTGAA GCCCAGTTTC AAAAAAATGC ACCATCTTCT 
GGGGCTTGGG GAGTCAATAA GATCATCTCT TCTTTGTCAT CTGCTTTTCC TGTATTTGAA 
GATGGAAACA AAGAAAATTA TGGATTACCA CAGCCGAAAA ATAAACCTGC AGGAGCCAGG 
ACCTTTGGAG AACGTTCCGT GAGCAGGTTT CCTGCAAAAC CAAATGAAGT GCCTCATGCT 
GACGAGTTTC TGGATGATTC AACCGTGTGG GGTGTCCGCT GCAACAAAAG CCTGGCACCC 
AGCCCCAGGA GCCCGGGAGA CTTCAGGTCC GCCGCGCAGC TGGCGTCTAC GCCCTTCCAC 
AGGCTTCCCG CGGACTTCGC GCACCCACTG GAAGATAAAG AAAATATGGT GGCGAACCAG 
CACACCCACG TGGCTGTGGA TCCTTGTGCA AGAAACAAGG TGGAACCCTT GAAAGAGAGA 
AGGTTCAGTC CAGTCGAAGA GACAAGCCCA GACCCAGTGT GCCCCACAGA GGGCCCTTCG 
GCGTCCTTGC TCCGTCTGGG CCAGCCTGCT GCAGCCGTAC TGGTGAGCGA GGCAGAGCAG 
GGTCTCAAGG CCTGTAAAGT CACAGACCCC GACTTGGCCC CTGCGGAGGA TCCGGCAGAA 
GCCAGCGCTG GGCTCCAGGC TGAACTGATG CAGGCTGGCC CGCCTGGGAA TGTCAACGCT 
CCAAACTTCA CTGTTGAGAA CCCCTGGGAT GAGGAGTTGA TTCTCAAACT TTTATCCGGG 
CTTTCTAAAC CAGTGAGTTC CTATCCAAAT ACTTATGAAT GGGCATGTAA GCTTCCAGCC 
ATCAAGCCCA AGACTGAATT CCAGTTAGGT TCTTTGCTGG TCTACGTTGA TCACTTTATT 
GGAGAAGGGG CTTTCGCCCA AGTCTATGAA GTTACCCATG GAGATGTGAA CAACAGCAAA 
AATAAGGAGA AATTTGCTTT GAAGGTCCAG AAGCCTCCCA ACCTCTGGGA GTTCTACATT 
GGGACCCAGC TGATGGAAAG ACTGAACCCA GGTGCGCAGC ACATGTTCAT CAGGTTCTAT 
TCGGCCCACC TGTTCCAGAA CGGCAGCGTG TTGGTGGGGG ACCTCTACAG CTACGGCACG 
CTGTTGAATG CCATTAATCT CTATAAAAAT ACCCCTGAAA AAGTGATGCC TCAGGCACTA 
GTCCTCGCTT TTGCCATCAG AATGCTCTGC ATGATCCAGC AAGTCCATGA CTGTGAAATC 
ATCCACGGAG ACATCAAGCC AGACAATTTC ATACTCGGAA ACAGATTTTT GGAACAAGAC 
AACAAAGATG ATGACTTATC TGCCGGCTTG GTCCTGATTG ACCTGGGTCA GAGTATAGAC 
ATGAAACTTT TTCCGAAAGG AACTGCATTT ACAGGGAAGT GTGAAACTTC TGGTTTCCAG 
TGTATTGAGA TGCTCAGTAA CAAGCCATGG AACTACCAGA TTGATTACTT TGGAGTTGCT 
GCAACCATAT ACTGCATGCT TTGTGGCACT TACATGAAAG TGAAAAATGA AGGGGGCATC 
TGGAAGCCTG AAGGTCTTTT TAGAAGGCTT CCTCACCTGG ACATGTGGAA TGAATTTTTT 
CACGTAATGT TGAATATACC CGATTGTCAT CATCTTCCAT CTTTGGATTT GTTAAGGCAG 
AAGCTAAAGA AAACATTTCA AGAACACTAC ACCAGCAAGA TCAAGACCCT GTGTAATAGG 
CTAATTGTAT TGCTCTTAGA ATATAAGCGT TCTCGGAAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BUB1 ( XM_012150066.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_012005456.1 Ovis aries musimon BUB1, mitotic checkpoint serine/threonine kinase (BUB1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OOv26088
Clone ID Related Accession (Same CDS sequence)	XM_012150066.2
Accession Version	XM_012150066.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3321bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	mitotic checkpoint serine/threonine-protein kinase BUB1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942623.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012150066.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGAGGTG CCCTCGGGGA CGCGTACCTG GGCCGGAAGA GCGGACAGCC GCCCAGGAGC 
CCGCGCAGCT CGTTTTTGAG GCTCCAGAAC TCGCCGTCCA GAATGTTTGA AGCCCATATG 
CAGAGCTACA AGGGCGATGA CCCACTTGGT GAATGGGAAA GTTACATGCG ATGGGTAGAA 
GAGAATTTTC CTGAGAATAA AGAATACTTA ACAACTTTAT TAGAGCATCT AATGAAGGAA 
TTTTTAGATA AGAAGAGATA TCACAATGAC CCAAGGTTCA TCAATTATTG TCTACAATTT 
GCCAAGTGCA ACAGTGACCT TCACCAGTTT TTTGAGTTTC TGCACAACCA CGGTATCGGA 
ACCCAGGCAG CTCCTCTGTA CGTAGCCTGG GCCGGGCACC TGGAAGGCCA GGGGGAGTGG 
CAGCACGCCA GTGCCGTTTT TCGGAGAGGA ATTCAGAACC AGGCCGAACC TAGAGAGCTG 
CTGCAACAAC AGTACAGGAT GTTTCAGACA CGCCTCACTG AAACCCATTT GTCTACTCAA 
GTGAGAACCT CAGAACCTCT GCATGATGCT CAGATTTTAA ATCAAATGGT AACATCAAAC 
TCAAATCCAG GAAGTAACTC AGCCTGCATT TCTAAGAATC AGGGTCCACA ACTTCCTGGA 
GCAACACCTG CAGCTGGTGA TAAAGGGTCA GACATGGAAC AAAGGGTGAT CACAATTTCT 
AAATCAGAAT ATTCTTCACA CGCATCTGTG GCAGACAAAG CTGATGTTCA GCAGGTTGTC 
ATGTATTGCA AGGAGAAGCT TATTCGCGGA GAATCGGAGT TTTCCTTCGA AGAGCTGAGA 
GCCCAGAGAT ACAATCTACG GAGAAAGCAT GAACAATGGG TAAATGAAGA GAGGCATTAT 
ATGAAAAGGA AAGAAGCAAA TGCTTTGGAA GAACAACTAT TAAAACAGAA AATGGATGAG 
CTTCATAAGA AGCTGCACCA GGTGGTGGAG ATGTCGCAGG GGAACCTGCC CTCTTCCCGG 
GGGACGTCTG AGGTCCGTCC AGCGTGTTTG GAGGCACACA CAGACTCCCA GCAGGAACTA 
AGAGCTCCGG GTCTTCCAGC CGTCCCTCAG CAGACCTCGA AGAACGCGGA CGAGAAGCCC 
GGGGAGGCAC CTTCTGTCCC TCTTACAGCA AGTGCTGTCG CCACTGCTTT GGTGTCCCCG 
GCCGCCAGCC AGGGTGTGGC TTGCCCGGCT CCTTTGGCAG ACCCGCCCGT GACGGACGCC 
GTGTTTTCAG GGGGCGGCAG AGATGCCAGG TGTGTGAACA AAAGTACTCA TGAATTCAGG 
CCACAGAGCG GAGCAGAGAT AAAAGAAGGG TGTGAAACAC AGAAGGTTGT TAACACAAGT 
TCCTTTCACA CAACTCCAAA CACATCTCTG GGACTGGTTC AGGCAACACC ATCCAAAGTG 
CAGCCATCAC CCACGGTGCA CACCAAGGAA GCTCTCGGTT TCATCATGAA TGTGTTTCAG 
GCTCCTACAC TTCCTGATAT TTCCGATGAC AAAGAGGAAT GGCCATCTCT AGGTCAAAAC 
GAGTTTGAAG CCCAGTTTCA AAAAAATGCA CCATCTTCTG GGGCTTGGGG AGTCAATAAG 
ATCATCTCTT CTTTGTCATC TGCTTTTCCT GTATTTGAAG ATGGAAACAA AGAAAATTAT 
GGATTACCAC AGCCGAAAAA TAAACCTGCA GGAGCCAGGA CCTTTGGAGA ACGTTCCGTG 
AGCAGGTTTC CTGCAAAACC AAATGAAGTG CCTCATGCTG ACGAGTTTCT GGATGATTCA 
ACCGTGTGGG GTGTCCGCTG CAACAAAAGC CTGGCACCCA GCCCCAGGAG CCCGGGAGAC 
TTCAGGTCCG CCGCGCAGCT GGCGTCTACG CCCTTCCACA GGCTTCCCGC GGACTTCGCG 
CACCCACTGG AAGATAAAGA AAATATGGTG GCGAACCAGC ACACCCACGT GGCTGTGGAT 
CCTTGTGCAA GAAACAAGGT GGAACCCTTG AAAGAGAGAA GGTTCAGTCC AGTCGAAGAG 
ACAAGCCCAG ACCCAGTGTG CCCCACAGAG GGCCCTTCGG CGTCCTTGCT CCGTCTGGGC 
CAGCCTGCTG CAGCCGTACT GGTGAGCGAG GCAGAGCAGG GTCTCAAGGC CTGTAAAGTC 
ACAGACCCCG ACTTGGCCCC TGCGGAGGAT CCGGCAGAAG CCAGCGCTGG GCTCCAGGCT 
GAACTGATGC AGGCTGGCCC GCCTGGGAAT GTCAACGCTC CAAACTTCAC TGTTGAGAAC 
CCCTGGGATG AGGAGTTGAT TCTCAAACTT TTATCCGGGC TTTCTAAACC AGTGAGTTCC 
TATCCAAATA CTTATGAATG GGCATGTAAG CTTCCAGCCA TCAAGCCCAA GACTGAATTC 
CAGTTAGGTT CTTTGCTGGT CTACGTTGAT CACTTTATTG GAGAAGGGGC TTTCGCCCAA 
GTCTATGAAG TTACCCATGG AGATGTGAAC AACAGCAAAA ATAAGGAGAA ATTTGCTTTG 
AAGGTCCAGA AGCCTCCCAA CCTCTGGGAG TTCTACATTG GGACCCAGCT GATGGAAAGA 
CTGAACCCAG GTGCGCAGCA CATGTTCATC AGGTTCTATT CGGCCCACCT GTTCCAGAAC 
GGCAGCGTGT TGGTGGGGGA CCTCTACAGC TACGGCACGC TGTTGAATGC CATTAATCTC 
TATAAAAATA CCCCTGAAAA AGTGATGCCT CAGGCACTAG TCCTCGCTTT TGCCATCAGA 
ATGCTCTGCA TGATCCAGCA AGTCCATGAC TGTGAAATCA TCCACGGAGA CATCAAGCCA 
GACAATTTCA TACTCGGAAA CAGATTTTTG GAACAAGACA ACAAAGATGA TGACTTATCT 
GCCGGCTTGG TCCTGATTGA CCTGGGTCAG AGTATAGACA TGAAACTTTT TCCGAAAGGA 
ACTGCATTTA CAGGGAAGTG TGAAACTTCT GGTTTCCAGT GTATTGAGAT GCTCAGTAAC 
AAGCCATGGA ACTACCAGAT TGATTACTTT GGAGTTGCTG CAACCATATA CTGCATGCTT 
TGTGGCACTT ACATGAAAGT GAAAAATGAA GGGGGCATCT GGAAGCCTGA AGGTCTTTTT 
AGAAGGCTTC CTCACCTGGA CATGTGGAAT GAATTTTTTC ACGTAATGTT GAATATACCC 
GATTGTCATC ATCTTCCATC TTTGGATTTG TTAAGGCAGA AGCTAAAGAA AACATTTCAA 
GAACACTACA CCAGCAAGAT CAAGACCCTG TGTAATAGGC TAATTGTATT GCTCTTAGAA 
TATAAGCGTT CTCGGAAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012005456.1
CDS	3328..6648
Translation	MRGALGDAYLGRKSGQPPRSPRSSFLRLQNSPSRMFEAHMQSYKGDDPLGEWESYMRWVEENFPENKEYLTTLLEHLMKEFLDKKRYHNDPRFINYCLQFAKCNSDLHQFFEFLHNHGIGTQAAPLYVAWAGHLEGQGEWQHASAVFRRGIQNQAEPRELLQQQYRMFQTRLTETHLSTQVRTSEPLHDAQILNQMVTSNSNPGSNSACISKNQGPQLPGATPAAGDKGSDMEQRVITISKSEYSSHASVADKADVQQVVMYCKEKLIRGESEFSFEELRAQRYNLRRKHEQWVNEERHYMKRKEANALEEQLLKQKMDELHKKLHQVVEMSQGNLPSSRGTSEVRPACLEAHTDSQQELRAPGLPAVPQQTSKNADEKPGEAPSVPLTASAVATALVSPAASQGVACPAPLADPPVTDAVFSGGGRDARCVNKSTHEFRPQSGAEIKEGCETQKVVNTSSFHTTPNTSLGLVQATPSKVQPSPTVHTKEALGFIMNVFQAPTLPDISDDKEEWPSLGQNEFEAQFQKNAPSSGAWGVNKIISSLSSAFPVFEDGNKENYGLPQPKNKPAGARTFGERSVSRFPAKPNEVPHADEFLDDSTVWGVRCNKSLAPSPRSPGDFRSAAQLASTPFHRLPADFAHPLEDKENMVANQHTHVAVDPCARNKVEPLKERRFSPVEETSPDPVCPTEGPSASLLRLGQPAAAVLVSEAEQGLKACKVTDPDLAPAEDPAEASAGLQAELMQAGPPGNVNAPNFTVENPWDEELILKLLSGLSKPVSSYPNTYEWACKLPAIKPKTEFQLGSLLVYVDHFIGEGAFAQVYEVTHGDVNNSKNKEKFALKVQKPPNLWEFYIGTQLMERLNPGAQHMFIRFYSAHLFQNGSVLVGDLYSYGTLLNAINLYKNTPEKVMPQALVLAFAIRMLCMIQQVHDCEIIHGDIKPDNFILGNRFLEQDNKDDDLSAGLVLIDLGQSIDMKLFPKGTAFTGKCETSGFQCIEMLSNKPWNYQIDYFGVAATIYCMLCGTYMKVKNEGGIWKPEGLFRRLPHLDMWNEFFHVMLNIPDCHHLPSLDLLRQKLKKTFQEHYTSKIKTLCNRLIVLLLEYKRSRK

Target ORF information:

RefSeq Version	XM_012150066.2
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon BUB1, mitotic checkpoint serine/threonine kinase (BUB1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012150066.2

ORF Insert Sequence:

ATGCGAGGTG CCCTCGGGGA CGCGTACCTG GGCCGGAAGA GCGGACAGCC GCCCAGGAGC 
CCGCGCAGCT CGTTTTTGAG GCTCCAGAAC TCGCCGTCCA GAATGTTTGA AGCCCATATG 
CAGAGCTACA AGGGCGATGA CCCACTTGGT GAATGGGAAA GTTACATGCG ATGGGTAGAA 
GAGAATTTTC CTGAGAATAA AGAATACTTA ACAACTTTAT TAGAGCATCT AATGAAGGAA 
TTTTTAGATA AGAAGAGATA TCACAATGAC CCAAGGTTCA TCAATTATTG TCTACAATTT 
GCCAAGTGCA ACAGTGACCT TCACCAGTTT TTTGAGTTTC TGCACAACCA CGGTATCGGA 
ACCCAGGCAG CTCCTCTGTA CGTAGCCTGG GCCGGGCACC TGGAAGGCCA GGGGGAGTGG 
CAGCACGCCA GTGCCGTTTT TCGGAGAGGA ATTCAGAACC AGGCCGAACC TAGAGAGCTG 
CTGCAACAAC AGTACAGGAT GTTTCAGACA CGCCTCACTG AAACCCATTT GTCTACTCAA 
GTGAGAACCT CAGAACCTCT GCATGATGCT CAGATTTTAA ATCAAATGGT AACATCAAAC 
TCAAATCCAG GAAGTAACTC AGCCTGCATT TCTAAGAATC AGGGTCCACA ACTTCCTGGA 
GCAACACCTG CAGCTGGTGA TAAAGGGTCA GACATGGAAC AAAGGGTGAT CACAATTTCT 
AAATCAGAAT ATTCTTCACA CGCATCTGTG GCAGACAAAG CTGATGTTCA GCAGGTTGTC 
ATGTATTGCA AGGAGAAGCT TATTCGCGGA GAATCGGAGT TTTCCTTCGA AGAGCTGAGA 
GCCCAGAGAT ACAATCTACG GAGAAAGCAT GAACAATGGG TAAATGAAGA GAGGCATTAT 
ATGAAAAGGA AAGAAGCAAA TGCTTTGGAA GAACAACTAT TAAAACAGAA AATGGATGAG 
CTTCATAAGA AGCTGCACCA GGTGGTGGAG ATGTCGCAGG GGAACCTGCC CTCTTCCCGG 
GGGACGTCTG AGGTCCGTCC AGCGTGTTTG GAGGCACACA CAGACTCCCA GCAGGAACTA 
AGAGCTCCGG GTCTTCCAGC CGTCCCTCAG CAGACCTCGA AGAACGCGGA CGAGAAGCCC 
GGGGAGGCAC CTTCTGTCCC TCTTACAGCA AGTGCTGTCG CCACTGCTTT GGTGTCCCCG 
GCCGCCAGCC AGGGTGTGGC TTGCCCGGCT CCTTTGGCAG ACCCGCCCGT GACGGACGCC 
GTGTTTTCAG GGGGCGGCAG AGATGCCAGG TGTGTGAACA AAAGTACTCA TGAATTCAGG 
CCACAGAGCG GAGCAGAGAT AAAAGAAGGG TGTGAAACAC AGAAGGTTGT TAACACAAGT 
TCCTTTCACA CAACTCCAAA CACATCTCTG GGACTGGTTC AGGCAACACC ATCCAAAGTG 
CAGCCATCAC CCACGGTGCA CACCAAGGAA GCTCTCGGTT TCATCATGAA TGTGTTTCAG 
GCTCCTACAC TTCCTGATAT TTCCGATGAC AAAGAGGAAT GGCCATCTCT AGGTCAAAAC 
GAGTTTGAAG CCCAGTTTCA AAAAAATGCA CCATCTTCTG GGGCTTGGGG AGTCAATAAG 
ATCATCTCTT CTTTGTCATC TGCTTTTCCT GTATTTGAAG ATGGAAACAA AGAAAATTAT 
GGATTACCAC AGCCGAAAAA TAAACCTGCA GGAGCCAGGA CCTTTGGAGA ACGTTCCGTG 
AGCAGGTTTC CTGCAAAACC AAATGAAGTG CCTCATGCTG ACGAGTTTCT GGATGATTCA 
ACCGTGTGGG GTGTCCGCTG CAACAAAAGC CTGGCACCCA GCCCCAGGAG CCCGGGAGAC 
TTCAGGTCCG CCGCGCAGCT GGCGTCTACG CCCTTCCACA GGCTTCCCGC GGACTTCGCG 
CACCCACTGG AAGATAAAGA AAATATGGTG GCGAACCAGC ACACCCACGT GGCTGTGGAT 
CCTTGTGCAA GAAACAAGGT GGAACCCTTG AAAGAGAGAA GGTTCAGTCC AGTCGAAGAG 
ACAAGCCCAG ACCCAGTGTG CCCCACAGAG GGCCCTTCGG CGTCCTTGCT CCGTCTGGGC 
CAGCCTGCTG CAGCCGTACT GGTGAGCGAG GCAGAGCAGG GTCTCAAGGC CTGTAAAGTC 
ACAGACCCCG ACTTGGCCCC TGCGGAGGAT CCGGCAGAAG CCAGCGCTGG GCTCCAGGCT 
GAACTGATGC AGGCTGGCCC GCCTGGGAAT GTCAACGCTC CAAACTTCAC TGTTGAGAAC 
CCCTGGGATG AGGAGTTGAT TCTCAAACTT TTATCCGGGC TTTCTAAACC AGTGAGTTCC 
TATCCAAATA CTTATGAATG GGCATGTAAG CTTCCAGCCA TCAAGCCCAA GACTGAATTC 
CAGTTAGGTT CTTTGCTGGT CTACGTTGAT CACTTTATTG GAGAAGGGGC TTTCGCCCAA 
GTCTATGAAG TTACCCATGG AGATGTGAAC AACAGCAAAA ATAAGGAGAA ATTTGCTTTG 
AAGGTCCAGA AGCCTCCCAA CCTCTGGGAG TTCTACATTG GGACCCAGCT GATGGAAAGA 
CTGAACCCAG GTGCGCAGCA CATGTTCATC AGGTTCTATT CGGCCCACCT GTTCCAGAAC 
GGCAGCGTGT TGGTGGGGGA CCTCTACAGC TACGGCACGC TGTTGAATGC CATTAATCTC 
TATAAAAATA CCCCTGAAAA AGTGATGCCT CAGGCACTAG TCCTCGCTTT TGCCATCAGA 
ATGCTCTGCA TGATCCAGCA AGTCCATGAC TGTGAAATCA TCCACGGAGA CATCAAGCCA 
GACAATTTCA TACTCGGAAA CAGATTTTTG GAACAAGACA ACAAAGATGA TGACTTATCT 
GCCGGCTTGG TCCTGATTGA CCTGGGTCAG AGTATAGACA TGAAACTTTT TCCGAAAGGA 
ACTGCATTTA CAGGGAAGTG TGAAACTTCT GGTTTCCAGT GTATTGAGAT GCTCAGTAAC 
AAGCCATGGA ACTACCAGAT TGATTACTTT GGAGTTGCTG CAACCATATA CTGCATGCTT 
TGTGGCACTT ACATGAAAGT GAAAAATGAA GGGGGCATCT GGAAGCCTGA AGGTCTTTTT 
AGAAGGCTTC CTCACCTGGA CATGTGGAAT GAATTTTTTC ACGTAATGTT GAATATACCC 
GATTGTCATC ATCTTCCATC TTTGGATTTG TTAAGGCAGA AGCTAAAGAA AACATTTCAA 
GAACACTACA CCAGCAAGAT CAAGACCCTG TGTAATAGGC TAATTGTATT GCTCTTAGAA 
TATAAGCGTT CTCGGAAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BUB1 ( XM_012150067.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_012005457.1 Ovis aries musimon BUB1, mitotic checkpoint serine/threonine kinase (BUB1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OOv26089
Clone ID Related Accession (Same CDS sequence)	XM_012150067.2
Accession Version	XM_012150067.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3246bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	mitotic checkpoint serine/threonine-protein kinase BUB1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942623.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012150067.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATAACC CGGAAAATGT GTTTCAAATG TTTGAAGCCC ATATGCAGAG CTACAAGGGC 
GATGACCCAC TTGGTGAATG GGAAAGTTAC ATGCGATGGG TAGAAGAGAA TTTTCCTGAG 
AATAAAGAAT ACTTAACAAC TTTATTAGAG CATCTAATGA AGGAATTTTT AGATAAGAAG 
AGATATCACA ATGACCCAAG GTTCATCAAT TATTGTCTAC AATTTGCCAA GTGCAACAGT 
GACCTTCACC AGTTTTTTGA GTTTCTGCAC AACCACGGTA TCGGAACCCA GGCAGCTCCT 
CTGTACGTAG CCTGGGCCGG GCACCTGGAA GGCCAGGGGG AGTGGCAGCA CGCCAGTGCC 
GTTTTTCGGA GAGGAATTCA GAACCAGGCC GAACCTAGAG AGCTGCTGCA ACAACAGTAC 
AGGATGTTTC AGACACGCCT CACTGAAACC CATTTGTCTA CTCAAGTGAG AACCTCAGAA 
CCTCTGCATG ATGCTCAGAT TTTAAATCAA ATGGTAACAT CAAACTCAAA TCCAGGAAGT 
AACTCAGCCT GCATTTCTAA GAATCAGGGT CCACAACTTC CTGGAGCAAC ACCTGCAGCT 
GGTGATAAAG GGTCAGACAT GGAACAAAGG GTGATCACAA TTTCTAAATC AGAATATTCT 
TCACACGCAT CTGTGGCAGA CAAAGCTGAT GTTCAGCAGG TTGTCATGTA TTGCAAGGAG 
AAGCTTATTC GCGGAGAATC GGAGTTTTCC TTCGAAGAGC TGAGAGCCCA GAGATACAAT 
CTACGGAGAA AGCATGAACA ATGGGTAAAT GAAGAGAGGC ATTATATGAA AAGGAAAGAA 
GCAAATGCTT TGGAAGAACA ACTATTAAAA CAGAAAATGG ATGAGCTTCA TAAGAAGCTG 
CACCAGGTGG TGGAGATGTC GCAGGGGAAC CTGCCCTCTT CCCGGGGGAC GTCTGAGGTC 
CGTCCAGCGT GTTTGGAGGC ACACACAGAC TCCCAGCAGG AACTAAGAGC TCCGGGTCTT 
CCAGCCGTCC CTCAGCAGAC CTCGAAGAAC GCGGACGAGA AGCCCGGGGA GGCACCTTCT 
GTCCCTCTTA CAGCAAGTGC TGTCGCCACT GCTTTGGTGT CCCCGGCCGC CAGCCAGGGT 
GTGGCTTGCC CGGCTCCTTT GGCAGACCCG CCCGTGACGG ACGCCGTGTT TTCAGGGGGC 
GGCAGAGATG CCAGGTGTGT GAACAAAAGT ACTCATGAAT TCAGGCCACA GAGCGGAGCA 
GAGATAAAAG AAGGGTGTGA AACACAGAAG GTTGTTAACA CAAGTTCCTT TCACACAACT 
CCAAACACAT CTCTGGGACT GGTTCAGGCA ACACCATCCA AAGTGCAGCC ATCACCCACG 
GTGCACACCA AGGAAGCTCT CGGTTTCATC ATGAATGTGT TTCAGGCTCC TACACTTCCT 
GATATTTCCG ATGACAAAGA GGAATGGCCA TCTCTAGGTC AAAACGAGTT TGAAGCCCAG 
TTTCAAAAAA ATGCACCATC TTCTGGGGCT TGGGGAGTCA ATAAGATCAT CTCTTCTTTG 
TCATCTGCTT TTCCTGTATT TGAAGATGGA AACAAAGAAA ATTATGGATT ACCACAGCCG 
AAAAATAAAC CTGCAGGAGC CAGGACCTTT GGAGAACGTT CCGTGAGCAG GTTTCCTGCA 
AAACCAAATG AAGTGCCTCA TGCTGACGAG TTTCTGGATG ATTCAACCGT GTGGGGTGTC 
CGCTGCAACA AAAGCCTGGC ACCCAGCCCC AGGAGCCCGG GAGACTTCAG GTCCGCCGCG 
CAGCTGGCGT CTACGCCCTT CCACAGGCTT CCCGCGGACT TCGCGCACCC ACTGGAAGAT 
AAAGAAAATA TGGTGGCGAA CCAGCACACC CACGTGGCTG TGGATCCTTG TGCAAGAAAC 
AAGGTGGAAC CCTTGAAAGA GAGAAGGTTC AGTCCAGTCG AAGAGACAAG CCCAGACCCA 
GTGTGCCCCA CAGAGGGCCC TTCGGCGTCC TTGCTCCGTC TGGGCCAGCC TGCTGCAGCC 
GTACTGGTGA GCGAGGCAGA GCAGGGTCTC AAGGCCTGTA AAGTCACAGA CCCCGACTTG 
GCCCCTGCGG AGGATCCGGC AGAAGCCAGC GCTGGGCTCC AGGCTGAACT GATGCAGGCT 
GGCCCGCCTG GGAATGTCAA CGCTCCAAAC TTCACTGTTG AGAACCCCTG GGATGAGGAG 
TTGATTCTCA AACTTTTATC CGGGCTTTCT AAACCAGTGA GTTCCTATCC AAATACTTAT 
GAATGGGCAT GTAAGCTTCC AGCCATCAAG CCCAAGACTG AATTCCAGTT AGGTTCTTTG 
CTGGTCTACG TTGATCACTT TATTGGAGAA GGGGCTTTCG CCCAAGTCTA TGAAGTTACC 
CATGGAGATG TGAACAACAG CAAAAATAAG GAGAAATTTG CTTTGAAGGT CCAGAAGCCT 
CCCAACCTCT GGGAGTTCTA CATTGGGACC CAGCTGATGG AAAGACTGAA CCCAGGTGCG 
CAGCACATGT TCATCAGGTT CTATTCGGCC CACCTGTTCC AGAACGGCAG CGTGTTGGTG 
GGGGACCTCT ACAGCTACGG CACGCTGTTG AATGCCATTA ATCTCTATAA AAATACCCCT 
GAAAAAGTGA TGCCTCAGGC ACTAGTCCTC GCTTTTGCCA TCAGAATGCT CTGCATGATC 
CAGCAAGTCC ATGACTGTGA AATCATCCAC GGAGACATCA AGCCAGACAA TTTCATACTC 
GGAAACAGAT TTTTGGAACA AGACAACAAA GATGATGACT TATCTGCCGG CTTGGTCCTG 
ATTGACCTGG GTCAGAGTAT AGACATGAAA CTTTTTCCGA AAGGAACTGC ATTTACAGGG 
AAGTGTGAAA CTTCTGGTTT CCAGTGTATT GAGATGCTCA GTAACAAGCC ATGGAACTAC 
CAGATTGATT ACTTTGGAGT TGCTGCAACC ATATACTGCA TGCTTTGTGG CACTTACATG 
AAAGTGAAAA ATGAAGGGGG CATCTGGAAG CCTGAAGGTC TTTTTAGAAG GCTTCCTCAC 
CTGGACATGT GGAATGAATT TTTTCACGTA ATGTTGAATA TACCCGATTG TCATCATCTT 
CCATCTTTGG ATTTGTTAAG GCAGAAGCTA AAGAAAACAT TTCAAGAACA CTACACCAGC 
AAGATCAAGA CCCTGTGTAA TAGGCTAATT GTATTGCTCT TAGAATATAA GCGTTCTCGG 
AAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012005457.1
CDS	529..3774
Translation	MDNPENVFQMFEAHMQSYKGDDPLGEWESYMRWVEENFPENKEYLTTLLEHLMKEFLDKKRYHNDPRFINYCLQFAKCNSDLHQFFEFLHNHGIGTQAAPLYVAWAGHLEGQGEWQHASAVFRRGIQNQAEPRELLQQQYRMFQTRLTETHLSTQVRTSEPLHDAQILNQMVTSNSNPGSNSACISKNQGPQLPGATPAAGDKGSDMEQRVITISKSEYSSHASVADKADVQQVVMYCKEKLIRGESEFSFEELRAQRYNLRRKHEQWVNEERHYMKRKEANALEEQLLKQKMDELHKKLHQVVEMSQGNLPSSRGTSEVRPACLEAHTDSQQELRAPGLPAVPQQTSKNADEKPGEAPSVPLTASAVATALVSPAASQGVACPAPLADPPVTDAVFSGGGRDARCVNKSTHEFRPQSGAEIKEGCETQKVVNTSSFHTTPNTSLGLVQATPSKVQPSPTVHTKEALGFIMNVFQAPTLPDISDDKEEWPSLGQNEFEAQFQKNAPSSGAWGVNKIISSLSSAFPVFEDGNKENYGLPQPKNKPAGARTFGERSVSRFPAKPNEVPHADEFLDDSTVWGVRCNKSLAPSPRSPGDFRSAAQLASTPFHRLPADFAHPLEDKENMVANQHTHVAVDPCARNKVEPLKERRFSPVEETSPDPVCPTEGPSASLLRLGQPAAAVLVSEAEQGLKACKVTDPDLAPAEDPAEASAGLQAELMQAGPPGNVNAPNFTVENPWDEELILKLLSGLSKPVSSYPNTYEWACKLPAIKPKTEFQLGSLLVYVDHFIGEGAFAQVYEVTHGDVNNSKNKEKFALKVQKPPNLWEFYIGTQLMERLNPGAQHMFIRFYSAHLFQNGSVLVGDLYSYGTLLNAINLYKNTPEKVMPQALVLAFAIRMLCMIQQVHDCEIIHGDIKPDNFILGNRFLEQDNKDDDLSAGLVLIDLGQSIDMKLFPKGTAFTGKCETSGFQCIEMLSNKPWNYQIDYFGVAATIYCMLCGTYMKVKNEGGIWKPEGLFRRLPHLDMWNEFFHVMLNIPDCHHLPSLDLLRQKLKKTFQEHYTSKIKTLCNRLIVLLLEYKRSRK

Target ORF information:

RefSeq Version	XM_012150067.2
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon BUB1, mitotic checkpoint serine/threonine kinase (BUB1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012150067.2

ORF Insert Sequence:

ATGGATAACC CGGAAAATGT GTTTCAAATG TTTGAAGCCC ATATGCAGAG CTACAAGGGC 
GATGACCCAC TTGGTGAATG GGAAAGTTAC ATGCGATGGG TAGAAGAGAA TTTTCCTGAG 
AATAAAGAAT ACTTAACAAC TTTATTAGAG CATCTAATGA AGGAATTTTT AGATAAGAAG 
AGATATCACA ATGACCCAAG GTTCATCAAT TATTGTCTAC AATTTGCCAA GTGCAACAGT 
GACCTTCACC AGTTTTTTGA GTTTCTGCAC AACCACGGTA TCGGAACCCA GGCAGCTCCT 
CTGTACGTAG CCTGGGCCGG GCACCTGGAA GGCCAGGGGG AGTGGCAGCA CGCCAGTGCC 
GTTTTTCGGA GAGGAATTCA GAACCAGGCC GAACCTAGAG AGCTGCTGCA ACAACAGTAC 
AGGATGTTTC AGACACGCCT CACTGAAACC CATTTGTCTA CTCAAGTGAG AACCTCAGAA 
CCTCTGCATG ATGCTCAGAT TTTAAATCAA ATGGTAACAT CAAACTCAAA TCCAGGAAGT 
AACTCAGCCT GCATTTCTAA GAATCAGGGT CCACAACTTC CTGGAGCAAC ACCTGCAGCT 
GGTGATAAAG GGTCAGACAT GGAACAAAGG GTGATCACAA TTTCTAAATC AGAATATTCT 
TCACACGCAT CTGTGGCAGA CAAAGCTGAT GTTCAGCAGG TTGTCATGTA TTGCAAGGAG 
AAGCTTATTC GCGGAGAATC GGAGTTTTCC TTCGAAGAGC TGAGAGCCCA GAGATACAAT 
CTACGGAGAA AGCATGAACA ATGGGTAAAT GAAGAGAGGC ATTATATGAA AAGGAAAGAA 
GCAAATGCTT TGGAAGAACA ACTATTAAAA CAGAAAATGG ATGAGCTTCA TAAGAAGCTG 
CACCAGGTGG TGGAGATGTC GCAGGGGAAC CTGCCCTCTT CCCGGGGGAC GTCTGAGGTC 
CGTCCAGCGT GTTTGGAGGC ACACACAGAC TCCCAGCAGG AACTAAGAGC TCCGGGTCTT 
CCAGCCGTCC CTCAGCAGAC CTCGAAGAAC GCGGACGAGA AGCCCGGGGA GGCACCTTCT 
GTCCCTCTTA CAGCAAGTGC TGTCGCCACT GCTTTGGTGT CCCCGGCCGC CAGCCAGGGT 
GTGGCTTGCC CGGCTCCTTT GGCAGACCCG CCCGTGACGG ACGCCGTGTT TTCAGGGGGC 
GGCAGAGATG CCAGGTGTGT GAACAAAAGT ACTCATGAAT TCAGGCCACA GAGCGGAGCA 
GAGATAAAAG AAGGGTGTGA AACACAGAAG GTTGTTAACA CAAGTTCCTT TCACACAACT 
CCAAACACAT CTCTGGGACT GGTTCAGGCA ACACCATCCA AAGTGCAGCC ATCACCCACG 
GTGCACACCA AGGAAGCTCT CGGTTTCATC ATGAATGTGT TTCAGGCTCC TACACTTCCT 
GATATTTCCG ATGACAAAGA GGAATGGCCA TCTCTAGGTC AAAACGAGTT TGAAGCCCAG 
TTTCAAAAAA ATGCACCATC TTCTGGGGCT TGGGGAGTCA ATAAGATCAT CTCTTCTTTG 
TCATCTGCTT TTCCTGTATT TGAAGATGGA AACAAAGAAA ATTATGGATT ACCACAGCCG 
AAAAATAAAC CTGCAGGAGC CAGGACCTTT GGAGAACGTT CCGTGAGCAG GTTTCCTGCA 
AAACCAAATG AAGTGCCTCA TGCTGACGAG TTTCTGGATG ATTCAACCGT GTGGGGTGTC 
CGCTGCAACA AAAGCCTGGC ACCCAGCCCC AGGAGCCCGG GAGACTTCAG GTCCGCCGCG 
CAGCTGGCGT CTACGCCCTT CCACAGGCTT CCCGCGGACT TCGCGCACCC ACTGGAAGAT 
AAAGAAAATA TGGTGGCGAA CCAGCACACC CACGTGGCTG TGGATCCTTG TGCAAGAAAC 
AAGGTGGAAC CCTTGAAAGA GAGAAGGTTC AGTCCAGTCG AAGAGACAAG CCCAGACCCA 
GTGTGCCCCA CAGAGGGCCC TTCGGCGTCC TTGCTCCGTC TGGGCCAGCC TGCTGCAGCC 
GTACTGGTGA GCGAGGCAGA GCAGGGTCTC AAGGCCTGTA AAGTCACAGA CCCCGACTTG 
GCCCCTGCGG AGGATCCGGC AGAAGCCAGC GCTGGGCTCC AGGCTGAACT GATGCAGGCT 
GGCCCGCCTG GGAATGTCAA CGCTCCAAAC TTCACTGTTG AGAACCCCTG GGATGAGGAG 
TTGATTCTCA AACTTTTATC CGGGCTTTCT AAACCAGTGA GTTCCTATCC AAATACTTAT 
GAATGGGCAT GTAAGCTTCC AGCCATCAAG CCCAAGACTG AATTCCAGTT AGGTTCTTTG 
CTGGTCTACG TTGATCACTT TATTGGAGAA GGGGCTTTCG CCCAAGTCTA TGAAGTTACC 
CATGGAGATG TGAACAACAG CAAAAATAAG GAGAAATTTG CTTTGAAGGT CCAGAAGCCT 
CCCAACCTCT GGGAGTTCTA CATTGGGACC CAGCTGATGG AAAGACTGAA CCCAGGTGCG 
CAGCACATGT TCATCAGGTT CTATTCGGCC CACCTGTTCC AGAACGGCAG CGTGTTGGTG 
GGGGACCTCT ACAGCTACGG CACGCTGTTG AATGCCATTA ATCTCTATAA AAATACCCCT 
GAAAAAGTGA TGCCTCAGGC ACTAGTCCTC GCTTTTGCCA TCAGAATGCT CTGCATGATC 
CAGCAAGTCC ATGACTGTGA AATCATCCAC GGAGACATCA AGCCAGACAA TTTCATACTC 
GGAAACAGAT TTTTGGAACA AGACAACAAA GATGATGACT TATCTGCCGG CTTGGTCCTG 
ATTGACCTGG GTCAGAGTAT AGACATGAAA CTTTTTCCGA AAGGAACTGC ATTTACAGGG 
AAGTGTGAAA CTTCTGGTTT CCAGTGTATT GAGATGCTCA GTAACAAGCC ATGGAACTAC 
CAGATTGATT ACTTTGGAGT TGCTGCAACC ATATACTGCA TGCTTTGTGG CACTTACATG 
AAAGTGAAAA ATGAAGGGGG CATCTGGAAG CCTGAAGGTC TTTTTAGAAG GCTTCCTCAC 
CTGGACATGT GGAATGAATT TTTTCACGTA ATGTTGAATA TACCCGATTG TCATCATCTT 
CCATCTTTGG ATTTGTTAAG GCAGAAGCTA AAGAAAACAT TTCAAGAACA CTACACCAGC 
AAGATCAAGA CCCTGTGTAA TAGGCTAATT GTATTGCTCT TAGAATATAA GCGTTCTCGG 
AAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BUB1 ( XM_012150069.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_012005459.1 Ovis aries musimon BUB1, mitotic checkpoint serine/threonine kinase (BUB1), transcript variant X2, mRNA.

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CloneID	OOv26090
Clone ID Related Accession (Same CDS sequence)	XM_012150069.2
Accession Version	XM_012150069.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3237bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	mitotic checkpoint serine/threonine-protein kinase BUB1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942623.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012150069.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATAACC CGGAAAATGT GTTTCAAATG TTTGAAGCCC ATATGCAGAG CTACAAGGGC 
GATGACCCAC TTGGTGAATG GGAAAGTTAC ATGCGATGGG TAGAAGAGAA TTTTCCTGAG 
AATAAAGAAT ACTTAACAAC TTTATTAGAG CATCTAATGA AGGAATTTTT AGATAAGAAG 
AGATATCACA ATGACCCAAG GTTCATCAAT TATTGTCTAC AATTTGCCAA GTGCAACAGT 
GACCTTCACC AGTTTTTTGA GTTTCTGCAC AACCACGGTA TCGGAACCCA GGCAGCTCCT 
CTGTACGTAG CCTGGGCCGG GCACCTGGAA GGCCAGGGGG AGTGGCAGCA CGCCAGTGCC 
GTTTTTCGGA GAGGAATTCA GAACCAGGCC GAACCTAGAG AGCTGCTGCA ACAACAGTAC 
AGGATGTTTC AGACACGCCT CACTGAAACC CATTTGTCTA CTCAAGTGAG AACCTCAGAA 
CCTCTGCATG ATGCTCAGAT TTTAAATCAA ATGGTAACAT CAAACTCAAA TCCAGGAAGT 
AACTCAGCCT GCATTTCTAA GAATCAGGGT CCACAACTTC CTGGAGCAAC ACCTGCAGCT 
GGTGATAAAG GGGAACAAAG GGTGATCACA ATTTCTAAAT CAGAATATTC TTCACACGCA 
TCTGTGGCAG ACAAAGCTGA TGTTCAGCAG GTTGTCATGT ATTGCAAGGA GAAGCTTATT 
CGCGGAGAAT CGGAGTTTTC CTTCGAAGAG CTGAGAGCCC AGAGATACAA TCTACGGAGA 
AAGCATGAAC AATGGGTAAA TGAAGAGAGG CATTATATGA AAAGGAAAGA AGCAAATGCT 
TTGGAAGAAC AACTATTAAA ACAGAAAATG GATGAGCTTC ATAAGAAGCT GCACCAGGTG 
GTGGAGATGT CGCAGGGGAA CCTGCCCTCT TCCCGGGGGA CGTCTGAGGT CCGTCCAGCG 
TGTTTGGAGG CACACACAGA CTCCCAGCAG GAACTAAGAG CTCCGGGTCT TCCAGCCGTC 
CCTCAGCAGA CCTCGAAGAA CGCGGACGAG AAGCCCGGGG AGGCACCTTC TGTCCCTCTT 
ACAGCAAGTG CTGTCGCCAC TGCTTTGGTG TCCCCGGCCG CCAGCCAGGG TGTGGCTTGC 
CCGGCTCCTT TGGCAGACCC GCCCGTGACG GACGCCGTGT TTTCAGGGGG CGGCAGAGAT 
GCCAGGTGTG TGAACAAAAG TACTCATGAA TTCAGGCCAC AGAGCGGAGC AGAGATAAAA 
GAAGGGTGTG AAACACAGAA GGTTGTTAAC ACAAGTTCCT TTCACACAAC TCCAAACACA 
TCTCTGGGAC TGGTTCAGGC AACACCATCC AAAGTGCAGC CATCACCCAC GGTGCACACC 
AAGGAAGCTC TCGGTTTCAT CATGAATGTG TTTCAGGCTC CTACACTTCC TGATATTTCC 
GATGACAAAG AGGAATGGCC ATCTCTAGGT CAAAACGAGT TTGAAGCCCA GTTTCAAAAA 
AATGCACCAT CTTCTGGGGC TTGGGGAGTC AATAAGATCA TCTCTTCTTT GTCATCTGCT 
TTTCCTGTAT TTGAAGATGG AAACAAAGAA AATTATGGAT TACCACAGCC GAAAAATAAA 
CCTGCAGGAG CCAGGACCTT TGGAGAACGT TCCGTGAGCA GGTTTCCTGC AAAACCAAAT 
GAAGTGCCTC ATGCTGACGA GTTTCTGGAT GATTCAACCG TGTGGGGTGT CCGCTGCAAC 
AAAAGCCTGG CACCCAGCCC CAGGAGCCCG GGAGACTTCA GGTCCGCCGC GCAGCTGGCG 
TCTACGCCCT TCCACAGGCT TCCCGCGGAC TTCGCGCACC CACTGGAAGA TAAAGAAAAT 
ATGGTGGCGA ACCAGCACAC CCACGTGGCT GTGGATCCTT GTGCAAGAAA CAAGGTGGAA 
CCCTTGAAAG AGAGAAGGTT CAGTCCAGTC GAAGAGACAA GCCCAGACCC AGTGTGCCCC 
ACAGAGGGCC CTTCGGCGTC CTTGCTCCGT CTGGGCCAGC CTGCTGCAGC CGTACTGGTG 
AGCGAGGCAG AGCAGGGTCT CAAGGCCTGT AAAGTCACAG ACCCCGACTT GGCCCCTGCG 
GAGGATCCGG CAGAAGCCAG CGCTGGGCTC CAGGCTGAAC TGATGCAGGC TGGCCCGCCT 
GGGAATGTCA ACGCTCCAAA CTTCACTGTT GAGAACCCCT GGGATGAGGA GTTGATTCTC 
AAACTTTTAT CCGGGCTTTC TAAACCAGTG AGTTCCTATC CAAATACTTA TGAATGGGCA 
TGTAAGCTTC CAGCCATCAA GCCCAAGACT GAATTCCAGT TAGGTTCTTT GCTGGTCTAC 
GTTGATCACT TTATTGGAGA AGGGGCTTTC GCCCAAGTCT ATGAAGTTAC CCATGGAGAT 
GTGAACAACA GCAAAAATAA GGAGAAATTT GCTTTGAAGG TCCAGAAGCC TCCCAACCTC 
TGGGAGTTCT ACATTGGGAC CCAGCTGATG GAAAGACTGA ACCCAGGTGC GCAGCACATG 
TTCATCAGGT TCTATTCGGC CCACCTGTTC CAGAACGGCA GCGTGTTGGT GGGGGACCTC 
TACAGCTACG GCACGCTGTT GAATGCCATT AATCTCTATA AAAATACCCC TGAAAAAGTG 
ATGCCTCAGG CACTAGTCCT CGCTTTTGCC ATCAGAATGC TCTGCATGAT CCAGCAAGTC 
CATGACTGTG AAATCATCCA CGGAGACATC AAGCCAGACA ATTTCATACT CGGAAACAGA 
TTTTTGGAAC AAGACAACAA AGATGATGAC TTATCTGCCG GCTTGGTCCT GATTGACCTG 
GGTCAGAGTA TAGACATGAA ACTTTTTCCG AAAGGAACTG CATTTACAGG GAAGTGTGAA 
ACTTCTGGTT TCCAGTGTAT TGAGATGCTC AGTAACAAGC CATGGAACTA CCAGATTGAT 
TACTTTGGAG TTGCTGCAAC CATATACTGC ATGCTTTGTG GCACTTACAT GAAAGTGAAA 
AATGAAGGGG GCATCTGGAA GCCTGAAGGT CTTTTTAGAA GGCTTCCTCA CCTGGACATG 
TGGAATGAAT TTTTTCACGT AATGTTGAAT ATACCCGATT GTCATCATCT TCCATCTTTG 
GATTTGTTAA GGCAGAAGCT AAAGAAAACA TTTCAAGAAC ACTACACCAG CAAGATCAAG 
ACCCTGTGTA ATAGGCTAAT TGTATTGCTC TTAGAATATA AGCGTTCTCG GAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012005459.1
CDS	529..3765
Translation	MDNPENVFQMFEAHMQSYKGDDPLGEWESYMRWVEENFPENKEYLTTLLEHLMKEFLDKKRYHNDPRFINYCLQFAKCNSDLHQFFEFLHNHGIGTQAAPLYVAWAGHLEGQGEWQHASAVFRRGIQNQAEPRELLQQQYRMFQTRLTETHLSTQVRTSEPLHDAQILNQMVTSNSNPGSNSACISKNQGPQLPGATPAAGDKGEQRVITISKSEYSSHASVADKADVQQVVMYCKEKLIRGESEFSFEELRAQRYNLRRKHEQWVNEERHYMKRKEANALEEQLLKQKMDELHKKLHQVVEMSQGNLPSSRGTSEVRPACLEAHTDSQQELRAPGLPAVPQQTSKNADEKPGEAPSVPLTASAVATALVSPAASQGVACPAPLADPPVTDAVFSGGGRDARCVNKSTHEFRPQSGAEIKEGCETQKVVNTSSFHTTPNTSLGLVQATPSKVQPSPTVHTKEALGFIMNVFQAPTLPDISDDKEEWPSLGQNEFEAQFQKNAPSSGAWGVNKIISSLSSAFPVFEDGNKENYGLPQPKNKPAGARTFGERSVSRFPAKPNEVPHADEFLDDSTVWGVRCNKSLAPSPRSPGDFRSAAQLASTPFHRLPADFAHPLEDKENMVANQHTHVAVDPCARNKVEPLKERRFSPVEETSPDPVCPTEGPSASLLRLGQPAAAVLVSEAEQGLKACKVTDPDLAPAEDPAEASAGLQAELMQAGPPGNVNAPNFTVENPWDEELILKLLSGLSKPVSSYPNTYEWACKLPAIKPKTEFQLGSLLVYVDHFIGEGAFAQVYEVTHGDVNNSKNKEKFALKVQKPPNLWEFYIGTQLMERLNPGAQHMFIRFYSAHLFQNGSVLVGDLYSYGTLLNAINLYKNTPEKVMPQALVLAFAIRMLCMIQQVHDCEIIHGDIKPDNFILGNRFLEQDNKDDDLSAGLVLIDLGQSIDMKLFPKGTAFTGKCETSGFQCIEMLSNKPWNYQIDYFGVAATIYCMLCGTYMKVKNEGGIWKPEGLFRRLPHLDMWNEFFHVMLNIPDCHHLPSLDLLRQKLKKTFQEHYTSKIKTLCNRLIVLLLEYKRSRK

Target ORF information:

RefSeq Version	XM_012150069.2
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon BUB1, mitotic checkpoint serine/threonine kinase (BUB1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012150069.2

ORF Insert Sequence:

ATGGATAACC CGGAAAATGT GTTTCAAATG TTTGAAGCCC ATATGCAGAG CTACAAGGGC 
GATGACCCAC TTGGTGAATG GGAAAGTTAC ATGCGATGGG TAGAAGAGAA TTTTCCTGAG 
AATAAAGAAT ACTTAACAAC TTTATTAGAG CATCTAATGA AGGAATTTTT AGATAAGAAG 
AGATATCACA ATGACCCAAG GTTCATCAAT TATTGTCTAC AATTTGCCAA GTGCAACAGT 
GACCTTCACC AGTTTTTTGA GTTTCTGCAC AACCACGGTA TCGGAACCCA GGCAGCTCCT 
CTGTACGTAG CCTGGGCCGG GCACCTGGAA GGCCAGGGGG AGTGGCAGCA CGCCAGTGCC 
GTTTTTCGGA GAGGAATTCA GAACCAGGCC GAACCTAGAG AGCTGCTGCA ACAACAGTAC 
AGGATGTTTC AGACACGCCT CACTGAAACC CATTTGTCTA CTCAAGTGAG AACCTCAGAA 
CCTCTGCATG ATGCTCAGAT TTTAAATCAA ATGGTAACAT CAAACTCAAA TCCAGGAAGT 
AACTCAGCCT GCATTTCTAA GAATCAGGGT CCACAACTTC CTGGAGCAAC ACCTGCAGCT 
GGTGATAAAG GGGAACAAAG GGTGATCACA ATTTCTAAAT CAGAATATTC TTCACACGCA 
TCTGTGGCAG ACAAAGCTGA TGTTCAGCAG GTTGTCATGT ATTGCAAGGA GAAGCTTATT 
CGCGGAGAAT CGGAGTTTTC CTTCGAAGAG CTGAGAGCCC AGAGATACAA TCTACGGAGA 
AAGCATGAAC AATGGGTAAA TGAAGAGAGG CATTATATGA AAAGGAAAGA AGCAAATGCT 
TTGGAAGAAC AACTATTAAA ACAGAAAATG GATGAGCTTC ATAAGAAGCT GCACCAGGTG 
GTGGAGATGT CGCAGGGGAA CCTGCCCTCT TCCCGGGGGA CGTCTGAGGT CCGTCCAGCG 
TGTTTGGAGG CACACACAGA CTCCCAGCAG GAACTAAGAG CTCCGGGTCT TCCAGCCGTC 
CCTCAGCAGA CCTCGAAGAA CGCGGACGAG AAGCCCGGGG AGGCACCTTC TGTCCCTCTT 
ACAGCAAGTG CTGTCGCCAC TGCTTTGGTG TCCCCGGCCG CCAGCCAGGG TGTGGCTTGC 
CCGGCTCCTT TGGCAGACCC GCCCGTGACG GACGCCGTGT TTTCAGGGGG CGGCAGAGAT 
GCCAGGTGTG TGAACAAAAG TACTCATGAA TTCAGGCCAC AGAGCGGAGC AGAGATAAAA 
GAAGGGTGTG AAACACAGAA GGTTGTTAAC ACAAGTTCCT TTCACACAAC TCCAAACACA 
TCTCTGGGAC TGGTTCAGGC AACACCATCC AAAGTGCAGC CATCACCCAC GGTGCACACC 
AAGGAAGCTC TCGGTTTCAT CATGAATGTG TTTCAGGCTC CTACACTTCC TGATATTTCC 
GATGACAAAG AGGAATGGCC ATCTCTAGGT CAAAACGAGT TTGAAGCCCA GTTTCAAAAA 
AATGCACCAT CTTCTGGGGC TTGGGGAGTC AATAAGATCA TCTCTTCTTT GTCATCTGCT 
TTTCCTGTAT TTGAAGATGG AAACAAAGAA AATTATGGAT TACCACAGCC GAAAAATAAA 
CCTGCAGGAG CCAGGACCTT TGGAGAACGT TCCGTGAGCA GGTTTCCTGC AAAACCAAAT 
GAAGTGCCTC ATGCTGACGA GTTTCTGGAT GATTCAACCG TGTGGGGTGT CCGCTGCAAC 
AAAAGCCTGG CACCCAGCCC CAGGAGCCCG GGAGACTTCA GGTCCGCCGC GCAGCTGGCG 
TCTACGCCCT TCCACAGGCT TCCCGCGGAC TTCGCGCACC CACTGGAAGA TAAAGAAAAT 
ATGGTGGCGA ACCAGCACAC CCACGTGGCT GTGGATCCTT GTGCAAGAAA CAAGGTGGAA 
CCCTTGAAAG AGAGAAGGTT CAGTCCAGTC GAAGAGACAA GCCCAGACCC AGTGTGCCCC 
ACAGAGGGCC CTTCGGCGTC CTTGCTCCGT CTGGGCCAGC CTGCTGCAGC CGTACTGGTG 
AGCGAGGCAG AGCAGGGTCT CAAGGCCTGT AAAGTCACAG ACCCCGACTT GGCCCCTGCG 
GAGGATCCGG CAGAAGCCAG CGCTGGGCTC CAGGCTGAAC TGATGCAGGC TGGCCCGCCT 
GGGAATGTCA ACGCTCCAAA CTTCACTGTT GAGAACCCCT GGGATGAGGA GTTGATTCTC 
AAACTTTTAT CCGGGCTTTC TAAACCAGTG AGTTCCTATC CAAATACTTA TGAATGGGCA 
TGTAAGCTTC CAGCCATCAA GCCCAAGACT GAATTCCAGT TAGGTTCTTT GCTGGTCTAC 
GTTGATCACT TTATTGGAGA AGGGGCTTTC GCCCAAGTCT ATGAAGTTAC CCATGGAGAT 
GTGAACAACA GCAAAAATAA GGAGAAATTT GCTTTGAAGG TCCAGAAGCC TCCCAACCTC 
TGGGAGTTCT ACATTGGGAC CCAGCTGATG GAAAGACTGA ACCCAGGTGC GCAGCACATG 
TTCATCAGGT TCTATTCGGC CCACCTGTTC CAGAACGGCA GCGTGTTGGT GGGGGACCTC 
TACAGCTACG GCACGCTGTT GAATGCCATT AATCTCTATA AAAATACCCC TGAAAAAGTG 
ATGCCTCAGG CACTAGTCCT CGCTTTTGCC ATCAGAATGC TCTGCATGAT CCAGCAAGTC 
CATGACTGTG AAATCATCCA CGGAGACATC AAGCCAGACA ATTTCATACT CGGAAACAGA 
TTTTTGGAAC AAGACAACAA AGATGATGAC TTATCTGCCG GCTTGGTCCT GATTGACCTG 
GGTCAGAGTA TAGACATGAA ACTTTTTCCG AAAGGAACTG CATTTACAGG GAAGTGTGAA 
ACTTCTGGTT TCCAGTGTAT TGAGATGCTC AGTAACAAGC CATGGAACTA CCAGATTGAT 
TACTTTGGAG TTGCTGCAAC CATATACTGC ATGCTTTGTG GCACTTACAT GAAAGTGAAA 
AATGAAGGGG GCATCTGGAA GCCTGAAGGT CTTTTTAGAA GGCTTCCTCA CCTGGACATG 
TGGAATGAAT TTTTTCACGT AATGTTGAAT ATACCCGATT GTCATCATCT TCCATCTTTG 
GATTTGTTAA GGCAGAAGCT AAAGAAAACA TTTCAAGAAC ACTACACCAG CAAGATCAAG 
ACCCTGTGTA ATAGGCTAAT TGTATTGCTC TTAGAATATA AGCGTTCTCG GAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BUB1 ( XM_012173898.3 ) cDNA ORF clone, Ovis aries(sheep) -> XP_012029288.2 Ovis aries BUB1 mitotic checkpoint serine/threonine kinase (BUB1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OSm77604
Clone ID Related Accession (Same CDS sequence)	XM_012173898.3
Accession Version	XM_012173898.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3237bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-05
Organism	Ovis aries(sheep)
Product	mitotic checkpoint serine/threonine-protein kinase BUB1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040254.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 6, 2019 this sequence version replaced XM_012173898.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ovis aries Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATAACC CGGAAAATGT GTTTCAAATG TTTGAAGCCC ATATGCAGAG CTACAAGGGC 
GATGACCCAC TTGGTGAATG GGAAAGTTAC ATGCGATGGG TAGAAGAGAA TTTTCCTGAG 
AATAAAGAAT ACTTAACAAC TTTATTAGAG CATCTAATGA AGGAATTTTT AGATAAGAAG 
AGATATCACA ATGACCCAAG GTTCATCAAT TATTGTCTAC AATTTGCCAA GTGCAACAGT 
GACCTTCACC AGTTTTTTGA GTTTCTGCAC AACCACGGTA TCGGAACCCA GGCAGCTCCT 
CTGTACGTAG CCTGGGCCGG GCACCTGGAA GGCCAGGGGG AGTGGCAGCA CGCCGGTGCC 
GTTTTTCGGA GAGGAATTCA GAACCAGGCC GAACCTACAG AGCTGCTGCA ACAACAGTAC 
AGGATGTTTC AGACACGCCT CACTGAAACC CATTTGTCTA CTCAAGTGAG AACCTCAGAA 
CCTCTGCATG ATGCTCAGAT TTTAAATCAA ATGGTAACAT CAAACTCAAA TCCAGGAAGT 
AACTCAGCCT GCATTTCTAA GAATCAGGGT CCACAACTTC CTGGAGCAAC ACCTGCAGCT 
GGTGATAAAG GGGAACAAAG GGTGATCACA ATTTCTAAAT CAGAATATTC TTCACACGCA 
TCTGTGGCAG ACAAAGCTGA TGTTCAGCAG GTTGTCATGT ATTGCAAGGA GAAGCTTATT 
CGCGGAGAAT CGGAGTTTTC CTTCGAAGAG CTGAGAGCCC AGAGATACAA TCTACGGAGA 
AAGCATGAAC AATGGGTAAA TGAAGAGAGG CATTATATGA AAAGGAAAGA AGCAAATGCT 
TTGGAAGAAC AACTATTAAA ACAGAAAATG GATGAGCTTC ATAAGAAGCT GCACCAGGTG 
GTGGAGATGT CGCAGGGGAA CCTGCCCTCT TCCCGGGGGA CGTCTGAGGT CCGTCCAGCG 
TGTTTGGAGG CACACACAGA CTCCCAGCAG GAACTAAGAG CTCCGGGTCT TCCAGCCGTC 
CCTCAGCAGA CCTCGAAGAA CGCGGACGAG AAGCCCGGGG AGGCACCTTC TGTCCCTCTT 
ACAGCAAGTG CTGTCGCCAC TGCTTTGGTG TCCCCGGCCG CCAGCCAGGG TGTGGCTTGC 
CCGGCTCCTT TGGCAGACCC GCCCGTGACG GACGCCGTGT TTTCAGGGGG CGGCAGAGAT 
GCCAGGTGTG TGAACAAAAG TACTCATGAA TTCAGGCCAC AGAGCGGAGC AGAGATAAAA 
GAAGGGTGTG AAACACAGAA GGTTGTTAAC ACAAGTTCCT TTCACACAAC TCCAAACACA 
TCTCTGGGAC TGGTTCAGGC AACACCATCC AAAGTGCAGC CATCACCCAC GGTGCACACC 
AAGGAAGCTC TCGGTTTCAT CATGAATGTG TTTCAGGCTC CTACACTTCC TGATATTTCC 
GATGACAAAG AGGAATGGCC ATCTCTAGGT CAAAACGAGT TTGAAGCCCA GTTTCAAAAA 
AATGCACCAT CTTCTGGGGC TTGGGGAGTC AATAAGATCA TCTCTTCTTT GTCATCTGCT 
TTTCCTGTAT TTGAAGATGG AAACAAAGAA AATTATGGAT TACCACAGCC GAAAAATAAA 
CCTGCAGGAG CCAGGACCTT TGGAGAACGT TCCGTGAGCA GGTTTCCTGC AAAACCAAAT 
GAAGTGCCTC ATGCTGACGA GTTTCTGGAT GATTCAACCG TGTGGGGTGT CCGCTGCAAC 
AAAAGCCTGG CACCCAGCCC CAGGAGCCCG GGAGACTTCA GGTCCGCCGC GCAGCTGGCG 
TCTACGCCCT TCCACAGGCT TCCCGCGGAC TCCGCGCACC CACTGGAAGA TAAAGAAAAT 
ATGGTGGCGA ACCAGCACAC CCACGTGGCT GTGGATCCTT GTGCAAGAAA CAAGGTGGAA 
CCCTTGAAAG AGAGAAGGTT CAGTCCAGTC GAAGAGACAA GCCCAGACCC AGTGTGCCCC 
ACAGAGGGCC CTTCGGCGTC CTTGCTCCGT CTGGGCCAGC CTGCTGCAGC CGTACTGATG 
AGCGAGGCAG AGCAGGGTCT CAAGGCCTGT AAAGTCACAG ACCCCGACTT GGCCACTGCA 
GAGGATCCGG CAGAAGCCAG CGCTGGGCTC CAGGCTGAAC TGATGCAGGC TGGCCCGCCT 
GGGACTGTCA ACGCTCCAAA CTTCACTGTT GAGAACCCCT GGGATGAGGA GTTGATTCTC 
AAACTTTTAT CCGGGCTTTC TAAACCAGTG AGTTCCTATC CAAATACTTA TGAATGGGCA 
TGTAAGCTTC CAGCCATCAA GCCCAAGACT GAATTCCAGT TAGGTTCTTT GCTGGTCTAC 
GTTGATCACT TTATTGGAGA AGGGGCTTTC GCCCAAGTCT ATGAAGTTAC CCATGGAGAT 
GTGAACAACA GCAAAAATAA GGAGAAATTT GCTTTGAAGG TCCAGAAGCC TCCCAACCTC 
TGGGAGTTCT ACATTGGGAC CCAGCTGATG GAGAGACTGA ACCCAGGTGC GCAGCACATG 
TTCATCAGGT TCTATTCGGC CCACCTGTTC CAGAACGGCA GCGTGTTGGT GGGGGACCTC 
TACAGCTACG GCACGCTGTT GAATGCCATT AATCTCTATA AAAATACCCC TGAAAAAGTG 
ATGCCTCAGG CACTAGTCCT CGCTTTTGCC ATCAGAATGC TCTGCATGAT CCAGCAAGTC 
CATGACTGTG AAATCATCCA CGGAGACATC AAGCCAGACA ATTTCATACT CGGAAACAGA 
TTTTTGGAAC AAGACAACAA AGATGATGAC TTATCTGCCG GCTTGGTCCT GATTGACCTG 
GGTCAGAGTA TAGACATGAA ACTTTTTCCG AAAGGAACTG CATTTACAGG GAAGTGTGAA 
ACTTCTGGTT TCCAGTGTAT TGAGATGCTC AGTAACAAGC CATGGAACTA CCAGATTGAT 
TACTTTGGAG TTGCTGCAAC CATATACTGC ATGCTTTGTG GCACTTACAT GAAAGTGAAA 
AATGAAGGGG GCATCTGGAA GCCTGAAGGT CTTTTTAGAA GGCTTCCTCA CCTGGACATG 
TGGAATGAGT TTTTTCACGT AATGTTGAAT ATACCCGATT GTCATCATCT TCCATCTTTG 
GATTTGTTAA GGCAGAAGCT AAAGAAAACA TTTCAAGAAC ACTACACCAG CAAGATCAAG 
ACCCTGTGTA ATAGGCTAAT TGTATTGCTC TTAGAATATA AGCGTTCTCG GAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012029288.2
CDS	67..3303
Translation	MDNPENVFQMFEAHMQSYKGDDPLGEWESYMRWVEENFPENKEYLTTLLEHLMKEFLDKKRYHNDPRFINYCLQFAKCNSDLHQFFEFLHNHGIGTQAAPLYVAWAGHLEGQGEWQHAGAVFRRGIQNQAEPTELLQQQYRMFQTRLTETHLSTQVRTSEPLHDAQILNQMVTSNSNPGSNSACISKNQGPQLPGATPAAGDKGEQRVITISKSEYSSHASVADKADVQQVVMYCKEKLIRGESEFSFEELRAQRYNLRRKHEQWVNEERHYMKRKEANALEEQLLKQKMDELHKKLHQVVEMSQGNLPSSRGTSEVRPACLEAHTDSQQELRAPGLPAVPQQTSKNADEKPGEAPSVPLTASAVATALVSPAASQGVACPAPLADPPVTDAVFSGGGRDARCVNKSTHEFRPQSGAEIKEGCETQKVVNTSSFHTTPNTSLGLVQATPSKVQPSPTVHTKEALGFIMNVFQAPTLPDISDDKEEWPSLGQNEFEAQFQKNAPSSGAWGVNKIISSLSSAFPVFEDGNKENYGLPQPKNKPAGARTFGERSVSRFPAKPNEVPHADEFLDDSTVWGVRCNKSLAPSPRSPGDFRSAAQLASTPFHRLPADSAHPLEDKENMVANQHTHVAVDPCARNKVEPLKERRFSPVEETSPDPVCPTEGPSASLLRLGQPAAAVLMSEAEQGLKACKVTDPDLATAEDPAEASAGLQAELMQAGPPGTVNAPNFTVENPWDEELILKLLSGLSKPVSSYPNTYEWACKLPAIKPKTEFQLGSLLVYVDHFIGEGAFAQVYEVTHGDVNNSKNKEKFALKVQKPPNLWEFYIGTQLMERLNPGAQHMFIRFYSAHLFQNGSVLVGDLYSYGTLLNAINLYKNTPEKVMPQALVLAFAIRMLCMIQQVHDCEIIHGDIKPDNFILGNRFLEQDNKDDDLSAGLVLIDLGQSIDMKLFPKGTAFTGKCETSGFQCIEMLSNKPWNYQIDYFGVAATIYCMLCGTYMKVKNEGGIWKPEGLFRRLPHLDMWNEFFHVMLNIPDCHHLPSLDLLRQKLKKTFQEHYTSKIKTLCNRLIVLLLEYKRSRK

Target ORF information:

RefSeq Version	XM_012173898.3
Organism	Ovis aries(sheep)
Definition	Ovis aries BUB1 mitotic checkpoint serine/threonine kinase (BUB1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012173898.3

ORF Insert Sequence:

ATGGATAACC CGGAAAATGT GTTTCAAATG TTTGAAGCCC ATATGCAGAG CTACAAGGGC 
GATGACCCAC TTGGTGAATG GGAAAGTTAC ATGCGATGGG TAGAAGAGAA TTTTCCTGAG 
AATAAAGAAT ACTTAACAAC TTTATTAGAG CATCTAATGA AGGAATTTTT AGATAAGAAG 
AGATATCACA ATGACCCAAG GTTCATCAAT TATTGTCTAC AATTTGCCAA GTGCAACAGT 
GACCTTCACC AGTTTTTTGA GTTTCTGCAC AACCACGGTA TCGGAACCCA GGCAGCTCCT 
CTGTACGTAG CCTGGGCCGG GCACCTGGAA GGCCAGGGGG AGTGGCAGCA CGCCGGTGCC 
GTTTTTCGGA GAGGAATTCA GAACCAGGCC GAACCTACAG AGCTGCTGCA ACAACAGTAC 
AGGATGTTTC AGACACGCCT CACTGAAACC CATTTGTCTA CTCAAGTGAG AACCTCAGAA 
CCTCTGCATG ATGCTCAGAT TTTAAATCAA ATGGTAACAT CAAACTCAAA TCCAGGAAGT 
AACTCAGCCT GCATTTCTAA GAATCAGGGT CCACAACTTC CTGGAGCAAC ACCTGCAGCT 
GGTGATAAAG GGGAACAAAG GGTGATCACA ATTTCTAAAT CAGAATATTC TTCACACGCA 
TCTGTGGCAG ACAAAGCTGA TGTTCAGCAG GTTGTCATGT ATTGCAAGGA GAAGCTTATT 
CGCGGAGAAT CGGAGTTTTC CTTCGAAGAG CTGAGAGCCC AGAGATACAA TCTACGGAGA 
AAGCATGAAC AATGGGTAAA TGAAGAGAGG CATTATATGA AAAGGAAAGA AGCAAATGCT 
TTGGAAGAAC AACTATTAAA ACAGAAAATG GATGAGCTTC ATAAGAAGCT GCACCAGGTG 
GTGGAGATGT CGCAGGGGAA CCTGCCCTCT TCCCGGGGGA CGTCTGAGGT CCGTCCAGCG 
TGTTTGGAGG CACACACAGA CTCCCAGCAG GAACTAAGAG CTCCGGGTCT TCCAGCCGTC 
CCTCAGCAGA CCTCGAAGAA CGCGGACGAG AAGCCCGGGG AGGCACCTTC TGTCCCTCTT 
ACAGCAAGTG CTGTCGCCAC TGCTTTGGTG TCCCCGGCCG CCAGCCAGGG TGTGGCTTGC 
CCGGCTCCTT TGGCAGACCC GCCCGTGACG GACGCCGTGT TTTCAGGGGG CGGCAGAGAT 
GCCAGGTGTG TGAACAAAAG TACTCATGAA TTCAGGCCAC AGAGCGGAGC AGAGATAAAA 
GAAGGGTGTG AAACACAGAA GGTTGTTAAC ACAAGTTCCT TTCACACAAC TCCAAACACA 
TCTCTGGGAC TGGTTCAGGC AACACCATCC AAAGTGCAGC CATCACCCAC GGTGCACACC 
AAGGAAGCTC TCGGTTTCAT CATGAATGTG TTTCAGGCTC CTACACTTCC TGATATTTCC 
GATGACAAAG AGGAATGGCC ATCTCTAGGT CAAAACGAGT TTGAAGCCCA GTTTCAAAAA 
AATGCACCAT CTTCTGGGGC TTGGGGAGTC AATAAGATCA TCTCTTCTTT GTCATCTGCT 
TTTCCTGTAT TTGAAGATGG AAACAAAGAA AATTATGGAT TACCACAGCC GAAAAATAAA 
CCTGCAGGAG CCAGGACCTT TGGAGAACGT TCCGTGAGCA GGTTTCCTGC AAAACCAAAT 
GAAGTGCCTC ATGCTGACGA GTTTCTGGAT GATTCAACCG TGTGGGGTGT CCGCTGCAAC 
AAAAGCCTGG CACCCAGCCC CAGGAGCCCG GGAGACTTCA GGTCCGCCGC GCAGCTGGCG 
TCTACGCCCT TCCACAGGCT TCCCGCGGAC TCCGCGCACC CACTGGAAGA TAAAGAAAAT 
ATGGTGGCGA ACCAGCACAC CCACGTGGCT GTGGATCCTT GTGCAAGAAA CAAGGTGGAA 
CCCTTGAAAG AGAGAAGGTT CAGTCCAGTC GAAGAGACAA GCCCAGACCC AGTGTGCCCC 
ACAGAGGGCC CTTCGGCGTC CTTGCTCCGT CTGGGCCAGC CTGCTGCAGC CGTACTGATG 
AGCGAGGCAG AGCAGGGTCT CAAGGCCTGT AAAGTCACAG ACCCCGACTT GGCCACTGCA 
GAGGATCCGG CAGAAGCCAG CGCTGGGCTC CAGGCTGAAC TGATGCAGGC TGGCCCGCCT 
GGGACTGTCA ACGCTCCAAA CTTCACTGTT GAGAACCCCT GGGATGAGGA GTTGATTCTC 
AAACTTTTAT CCGGGCTTTC TAAACCAGTG AGTTCCTATC CAAATACTTA TGAATGGGCA 
TGTAAGCTTC CAGCCATCAA GCCCAAGACT GAATTCCAGT TAGGTTCTTT GCTGGTCTAC 
GTTGATCACT TTATTGGAGA AGGGGCTTTC GCCCAAGTCT ATGAAGTTAC CCATGGAGAT 
GTGAACAACA GCAAAAATAA GGAGAAATTT GCTTTGAAGG TCCAGAAGCC TCCCAACCTC 
TGGGAGTTCT ACATTGGGAC CCAGCTGATG GAGAGACTGA ACCCAGGTGC GCAGCACATG 
TTCATCAGGT TCTATTCGGC CCACCTGTTC CAGAACGGCA GCGTGTTGGT GGGGGACCTC 
TACAGCTACG GCACGCTGTT GAATGCCATT AATCTCTATA AAAATACCCC TGAAAAAGTG 
ATGCCTCAGG CACTAGTCCT CGCTTTTGCC ATCAGAATGC TCTGCATGAT CCAGCAAGTC 
CATGACTGTG AAATCATCCA CGGAGACATC AAGCCAGACA ATTTCATACT CGGAAACAGA 
TTTTTGGAAC AAGACAACAA AGATGATGAC TTATCTGCCG GCTTGGTCCT GATTGACCTG 
GGTCAGAGTA TAGACATGAA ACTTTTTCCG AAAGGAACTG CATTTACAGG GAAGTGTGAA 
ACTTCTGGTT TCCAGTGTAT TGAGATGCTC AGTAACAAGC CATGGAACTA CCAGATTGAT 
TACTTTGGAG TTGCTGCAAC CATATACTGC ATGCTTTGTG GCACTTACAT GAAAGTGAAA 
AATGAAGGGG GCATCTGGAA GCCTGAAGGT CTTTTTAGAA GGCTTCCTCA CCTGGACATG 
TGGAATGAGT TTTTTCACGT AATGTTGAAT ATACCCGATT GTCATCATCT TCCATCTTTG 
GATTTGTTAA GGCAGAAGCT AAAGAAAACA TTTCAAGAAC ACTACACCAG CAAGATCAAG 
ACCCTGTGTA ATAGGCTAAT TGTATTGCTC TTAGAATATA AGCGTTCTCG GAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BUB1 ( XM_004006184.3 ) cDNA ORF clone, Ovis aries(sheep) -> XP_004006233.1 Ovis aries BUB1, mitotic checkpoint serine/threonine kinase (BUB1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OSm04132
Clone ID Related Accession (Same CDS sequence)	XM_004006184.3
Accession Version	XM_004006184.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3246bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries(sheep)
Product	mitotic checkpoint serine/threonine-protein kinase BUB1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014639012.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_004006184.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATAACC CGGAAAATGT GTTTCAAATG TTTGAAGCCC ATATGCAGAG CTACAAGGGC 
GATGACCCAC TTGGTGAATG GGAAAGTTAC ATGCGATGGG TAGAAGAGAA TTTTCCTGAG 
AATAAAGAAT ACTTAACAAC TTTATTAGAG CATCTAATGA AGGAATTTTT AGATAAGAAG 
AGATATCACA ATGACCCAAG GTTCATCAAT TATTGTCTAC AATTTGCCAA GTGCAACAGT 
GACCTTCACC AGTTTTTTGA GTTTCTGCAC AACCACGGTA TCGGAACCCA GGCAGCTCCT 
CTGTACGTAG CCTGGGCCGG GCACCTGGAA GGCCAGGGGG AGTGGCAGCA CGCCGGTGCC 
GTTTTTCGGA GAGGAATTCA GAACCAGGCC GAACCTACAG AGCTGCTGCA ACAACAGTAC 
AGGATGTTTC AGACACGCCT CACTGAAACC CATTTGTCTA CTCAAGTGAG AACCTCAGAA 
CCTCTGCATG ATGCTCAGAT TTTAAATCAA ATGGTAACAT CAAACTCAAA TCCAGGAAGT 
AACTCAGCCT GCATTTCTAA GAATCAGGGT CCACAACTTC CTGGAGCAAC ACCTGCAGCT 
GGTGATAAAG GGTCAGACAT GGAACAAAGG GTGATCACAA TTTCTAAATC AGAATATTCT 
TCACACGCAT CTGTGGCAGA CAAAGCTGAT GTTCAGCAGG TTGTCATGTA TTGCAAGGAG 
AAGCTTATTC GCGGAGAATC GGAGTTTTCC TTCGAAGAGC TGAGAGCCCA GAGATACAAT 
CTACGGAGAA AGCATGAACA ATGGGTAAAT GAAGAGAGGC ATTATATGAA AAGGAAAGAA 
GCAAATGCTT TGGAAGAACA ACTATTAAAA CAGAAAATGG ATGAGCTTCA TAAGAAGCTG 
CACCAGGTGG TGGAGATGTC GCAGGGGAAC CTGCCCTCTT CCCGGGGGAC GTCTGAGGTC 
CGTCCAGCGT GTTTGGAGGC ACACACAGAC TCCCAGCAGG AACTAAGAGC TCCGGGTCTT 
CCAGCCGTCC CTCAGCAGAC CTCGAAGAAC GCGGACGAGA AGCCCGGGGA GGCACCTTCT 
GTCCCTCTTA CGGCAAGTGC TGTCGCCACT GCTTTGGTGT CCCCGGCCGC CAGCCAGGGT 
GTGGCTTGCC CGGCTCCTTT GGCAGGCCCG CCCGTGACGG ACGCCATGTT TTCAGGGGGC 
GGCAGAGATG CCAGGTGTGT GAACAAAAGT ACTCATGAAT TCAGGCCACA GAGCGGAGCA 
GAGATAAAAG AAGGGTGTGA AACACAGAAG GTTGTTAACA CAAGTTCCTT TCACACAACT 
CCAAACACAT CTCTGGGACT GGTTCAGGCA ACACCATCCA AAGTGCAGCC ATCACCCACG 
GTGCACACCA AGGAAGCTCT CGGTTTCATC ATGAATGTGT TTCAGGCTCC TACACTTCCT 
GATATTTCCG ATGACAAAGA GGAATGGCCA TCTCTAGGTC AAAACGAGTT TGAAGCCCAG 
TTTCAAAAAA ATGCACCATC TTCTGGGGCT TGGGGAGTCA ATAAGATCAT CTCTTCTTTG 
TCATCTGCTT TTCCTGTATT TGAAGATGGA AACAAAGAAA ATTATGGCTT ACCACAGCCG 
AAAAATAAAC CTGCAGGAGC CAGGACCTTT GGAGAACGTT CCGTGAGCAG GTTTCCTGCA 
AAACCAAATG AAGTGCCTCA TGCTGACGAG TTTCTGGATG ATTCAACCGT GTGGGGTGTC 
CGCTGCAACA AAAGCCTGGC ACCCAGCCCC AGGAGCCCGG GAGACTTCAG GTCCGCCGCG 
CAGCTGGCGT CTACGCCCTT CCACAGGCTT CCCGCGGACT CCGCGCACCC ACTGGAAGAT 
AAAGAAAATA TGGTGGCGAA CCAGCACACC CACGTGGCTG TGGATCCTTG TGCAAGAAAC 
AAGGTGGAAC CCTTGAAAGA GAGAAGGTTC AGTCCAGTCG AAGAGACAAG CCCAGACCCA 
GTGTGCCCCA CAGAGGGCCC TTCGGCGTCC TTGCTCCGTC TGGGCCAGCC TGCTGCAGCC 
GTACTGGTGA GCGAGGCAGA GCAGGGTCTC AAGGCCTGTA AAGTCACAGA CCCCGACTTG 
GCCACTGCAG AGGATCCGGC AGAAGCCAGC GCTGGGCTCC AGGCTGAACT GATGCAGGCT 
GGCCCGCCTG GGACTGTCAA CGCTCCAAAC TTCACTGTTG AGAACCCCTG GGATGAGGAG 
TTGATTCTCA AACTTTTATC CGGGCTTTCT AAACCAGTGA GTTCCTATCC AAATACTTAT 
GAATGGGCAT GTAAGCTTCC AGCCATCAAG CCCAAGACTG AATTCCAGTT AGGTTCTTTG 
CTGGTCTACG TTGATCACTT TATTGGAGAA GGGGCTTTTG CCCAAGTCTA TGAAGTTACC 
CATGGAGATG TGAACAACAG CAAAAATAAG GAGAAATTTG CTTTGAAGGT CCAGAAGCCT 
CCCAACCTCT GGGAGTTCTA CATTGGGACC CAGCTGATGG AGAGACTGAA CCCAGGTGCG 
CAGCACATGT TCATCAGGTT CTATTCGGCC CACCTGTTCC AGAACGGCAG CGTGTTGGTG 
GGGGACCTCT ACAGCTACGG CACGCTGTTG AATGCCATTA ATCTCTATAA AAATACCCCT 
GAAAAAGTGA TGCCTCAGGC ACTAGTCCTC GCTTTTGCCA TCAGAATGCT CTGCATGATC 
CAGCAAGTCC ATGACTGTGA AATCATCCAC GGAGACATCA AGCCAGACAA TTTCATACTC 
GGAAACAGAT TTTTGGAACA AGACAACAAA GATGATGACT TATCTGCCGG CTTGGTCCTG 
ATTGACCTGG GTCAGAGTAT AGACATGAAA CTTTTTCCGA AAGGAACTGC ATTTACAGGG 
AAGTGTGAAA CTTCTGGTTT CCAGTGTATT GAGATGCTCA GTAACAAGCC ATGGAACTAC 
CAGATTGATT ACTTTGGAGT TGCTGCAACC ATATACTGCA TGCTTTGTGG CACTTACATG 
AAAGTGAAAA ATGAAGGGGG CATCTGGAAG CCTGAAGGTC TTTTTAGAAG GCTTCCTCAC 
CTGGACATGT GGAATGAGTT TTTTCACGTA ATGTTGAATA TACCCGATTG TCATCATCTT 
CCATCTTTGG ATTTGTTAAG GCAGAAGCTA AAGAAAACAT TTCAAGAACA CTACACCAGC 
AAGATCAAGA CCCTGTGTAA CAGGCTAATT GTATTGCTCT TAGAATATAA GCGTTCTCGG 
AAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004006233.1
CDS	529..3774
Translation	MDNPENVFQMFEAHMQSYKGDDPLGEWESYMRWVEENFPENKEYLTTLLEHLMKEFLDKKRYHNDPRFINYCLQFAKCNSDLHQFFEFLHNHGIGTQAAPLYVAWAGHLEGQGEWQHAGAVFRRGIQNQAEPTELLQQQYRMFQTRLTETHLSTQVRTSEPLHDAQILNQMVTSNSNPGSNSACISKNQGPQLPGATPAAGDKGSDMEQRVITISKSEYSSHASVADKADVQQVVMYCKEKLIRGESEFSFEELRAQRYNLRRKHEQWVNEERHYMKRKEANALEEQLLKQKMDELHKKLHQVVEMSQGNLPSSRGTSEVRPACLEAHTDSQQELRAPGLPAVPQQTSKNADEKPGEAPSVPLTASAVATALVSPAASQGVACPAPLAGPPVTDAMFSGGGRDARCVNKSTHEFRPQSGAEIKEGCETQKVVNTSSFHTTPNTSLGLVQATPSKVQPSPTVHTKEALGFIMNVFQAPTLPDISDDKEEWPSLGQNEFEAQFQKNAPSSGAWGVNKIISSLSSAFPVFEDGNKENYGLPQPKNKPAGARTFGERSVSRFPAKPNEVPHADEFLDDSTVWGVRCNKSLAPSPRSPGDFRSAAQLASTPFHRLPADSAHPLEDKENMVANQHTHVAVDPCARNKVEPLKERRFSPVEETSPDPVCPTEGPSASLLRLGQPAAAVLVSEAEQGLKACKVTDPDLATAEDPAEASAGLQAELMQAGPPGTVNAPNFTVENPWDEELILKLLSGLSKPVSSYPNTYEWACKLPAIKPKTEFQLGSLLVYVDHFIGEGAFAQVYEVTHGDVNNSKNKEKFALKVQKPPNLWEFYIGTQLMERLNPGAQHMFIRFYSAHLFQNGSVLVGDLYSYGTLLNAINLYKNTPEKVMPQALVLAFAIRMLCMIQQVHDCEIIHGDIKPDNFILGNRFLEQDNKDDDLSAGLVLIDLGQSIDMKLFPKGTAFTGKCETSGFQCIEMLSNKPWNYQIDYFGVAATIYCMLCGTYMKVKNEGGIWKPEGLFRRLPHLDMWNEFFHVMLNIPDCHHLPSLDLLRQKLKKTFQEHYTSKIKTLCNRLIVLLLEYKRSRK

Target ORF information:

RefSeq Version	XM_004006184.3
Organism	Ovis aries(sheep)
Definition	Ovis aries BUB1, mitotic checkpoint serine/threonine kinase (BUB1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004006184.3

ORF Insert Sequence:

ATGGATAACC CGGAAAATGT GTTTCAAATG TTTGAAGCCC ATATGCAGAG CTACAAGGGC 
GATGACCCAC TTGGTGAATG GGAAAGTTAC ATGCGATGGG TAGAAGAGAA TTTTCCTGAG 
AATAAAGAAT ACTTAACAAC TTTATTAGAG CATCTAATGA AGGAATTTTT AGATAAGAAG 
AGATATCACA ATGACCCAAG GTTCATCAAT TATTGTCTAC AATTTGCCAA GTGCAACAGT 
GACCTTCACC AGTTTTTTGA GTTTCTGCAC AACCACGGTA TCGGAACCCA GGCAGCTCCT 
CTGTACGTAG CCTGGGCCGG GCACCTGGAA GGCCAGGGGG AGTGGCAGCA CGCCGGTGCC 
GTTTTTCGGA GAGGAATTCA GAACCAGGCC GAACCTACAG AGCTGCTGCA ACAACAGTAC 
AGGATGTTTC AGACACGCCT CACTGAAACC CATTTGTCTA CTCAAGTGAG AACCTCAGAA 
CCTCTGCATG ATGCTCAGAT TTTAAATCAA ATGGTAACAT CAAACTCAAA TCCAGGAAGT 
AACTCAGCCT GCATTTCTAA GAATCAGGGT CCACAACTTC CTGGAGCAAC ACCTGCAGCT 
GGTGATAAAG GGTCAGACAT GGAACAAAGG GTGATCACAA TTTCTAAATC AGAATATTCT 
TCACACGCAT CTGTGGCAGA CAAAGCTGAT GTTCAGCAGG TTGTCATGTA TTGCAAGGAG 
AAGCTTATTC GCGGAGAATC GGAGTTTTCC TTCGAAGAGC TGAGAGCCCA GAGATACAAT 
CTACGGAGAA AGCATGAACA ATGGGTAAAT GAAGAGAGGC ATTATATGAA AAGGAAAGAA 
GCAAATGCTT TGGAAGAACA ACTATTAAAA CAGAAAATGG ATGAGCTTCA TAAGAAGCTG 
CACCAGGTGG TGGAGATGTC GCAGGGGAAC CTGCCCTCTT CCCGGGGGAC GTCTGAGGTC 
CGTCCAGCGT GTTTGGAGGC ACACACAGAC TCCCAGCAGG AACTAAGAGC TCCGGGTCTT 
CCAGCCGTCC CTCAGCAGAC CTCGAAGAAC GCGGACGAGA AGCCCGGGGA GGCACCTTCT 
GTCCCTCTTA CGGCAAGTGC TGTCGCCACT GCTTTGGTGT CCCCGGCCGC CAGCCAGGGT 
GTGGCTTGCC CGGCTCCTTT GGCAGGCCCG CCCGTGACGG ACGCCATGTT TTCAGGGGGC 
GGCAGAGATG CCAGGTGTGT GAACAAAAGT ACTCATGAAT TCAGGCCACA GAGCGGAGCA 
GAGATAAAAG AAGGGTGTGA AACACAGAAG GTTGTTAACA CAAGTTCCTT TCACACAACT 
CCAAACACAT CTCTGGGACT GGTTCAGGCA ACACCATCCA AAGTGCAGCC ATCACCCACG 
GTGCACACCA AGGAAGCTCT CGGTTTCATC ATGAATGTGT TTCAGGCTCC TACACTTCCT 
GATATTTCCG ATGACAAAGA GGAATGGCCA TCTCTAGGTC AAAACGAGTT TGAAGCCCAG 
TTTCAAAAAA ATGCACCATC TTCTGGGGCT TGGGGAGTCA ATAAGATCAT CTCTTCTTTG 
TCATCTGCTT TTCCTGTATT TGAAGATGGA AACAAAGAAA ATTATGGCTT ACCACAGCCG 
AAAAATAAAC CTGCAGGAGC CAGGACCTTT GGAGAACGTT CCGTGAGCAG GTTTCCTGCA 
AAACCAAATG AAGTGCCTCA TGCTGACGAG TTTCTGGATG ATTCAACCGT GTGGGGTGTC 
CGCTGCAACA AAAGCCTGGC ACCCAGCCCC AGGAGCCCGG GAGACTTCAG GTCCGCCGCG 
CAGCTGGCGT CTACGCCCTT CCACAGGCTT CCCGCGGACT CCGCGCACCC ACTGGAAGAT 
AAAGAAAATA TGGTGGCGAA CCAGCACACC CACGTGGCTG TGGATCCTTG TGCAAGAAAC 
AAGGTGGAAC CCTTGAAAGA GAGAAGGTTC AGTCCAGTCG AAGAGACAAG CCCAGACCCA 
GTGTGCCCCA CAGAGGGCCC TTCGGCGTCC TTGCTCCGTC TGGGCCAGCC TGCTGCAGCC 
GTACTGGTGA GCGAGGCAGA GCAGGGTCTC AAGGCCTGTA AAGTCACAGA CCCCGACTTG 
GCCACTGCAG AGGATCCGGC AGAAGCCAGC GCTGGGCTCC AGGCTGAACT GATGCAGGCT 
GGCCCGCCTG GGACTGTCAA CGCTCCAAAC TTCACTGTTG AGAACCCCTG GGATGAGGAG 
TTGATTCTCA AACTTTTATC CGGGCTTTCT AAACCAGTGA GTTCCTATCC AAATACTTAT 
GAATGGGCAT GTAAGCTTCC AGCCATCAAG CCCAAGACTG AATTCCAGTT AGGTTCTTTG 
CTGGTCTACG TTGATCACTT TATTGGAGAA GGGGCTTTTG CCCAAGTCTA TGAAGTTACC 
CATGGAGATG TGAACAACAG CAAAAATAAG GAGAAATTTG CTTTGAAGGT CCAGAAGCCT 
CCCAACCTCT GGGAGTTCTA CATTGGGACC CAGCTGATGG AGAGACTGAA CCCAGGTGCG 
CAGCACATGT TCATCAGGTT CTATTCGGCC CACCTGTTCC AGAACGGCAG CGTGTTGGTG 
GGGGACCTCT ACAGCTACGG CACGCTGTTG AATGCCATTA ATCTCTATAA AAATACCCCT 
GAAAAAGTGA TGCCTCAGGC ACTAGTCCTC GCTTTTGCCA TCAGAATGCT CTGCATGATC 
CAGCAAGTCC ATGACTGTGA AATCATCCAC GGAGACATCA AGCCAGACAA TTTCATACTC 
GGAAACAGAT TTTTGGAACA AGACAACAAA GATGATGACT TATCTGCCGG CTTGGTCCTG 
ATTGACCTGG GTCAGAGTAT AGACATGAAA CTTTTTCCGA AAGGAACTGC ATTTACAGGG 
AAGTGTGAAA CTTCTGGTTT CCAGTGTATT GAGATGCTCA GTAACAAGCC ATGGAACTAC 
CAGATTGATT ACTTTGGAGT TGCTGCAACC ATATACTGCA TGCTTTGTGG CACTTACATG 
AAAGTGAAAA ATGAAGGGGG CATCTGGAAG CCTGAAGGTC TTTTTAGAAG GCTTCCTCAC 
CTGGACATGT GGAATGAGTT TTTTCACGTA ATGTTGAATA TACCCGATTG TCATCATCTT 
CCATCTTTGG ATTTGTTAAG GCAGAAGCTA AAGAAAACAT TTCAAGAACA CTACACCAGC 
AAGATCAAGA CCCTGTGTAA CAGGCTAATT GTATTGCTCT TAGAATATAA GCGTTCTCGG 
AAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BUB1 ( XM_012173898.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_012029288.1 Ovis aries BUB1, mitotic checkpoint serine/threonine kinase (BUB1), transcript variant X2, mRNA.

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CloneID	OSm38428
Clone ID Related Accession (Same CDS sequence)	XM_012173898.2
Accession Version	XM_012173898.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3237bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries(sheep)
Product	mitotic checkpoint serine/threonine-protein kinase BUB1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014639012.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012173898.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATAACC CGGAAAATGT GTTTCAAATG TTTGAAGCCC ATATGCAGAG CTACAAGGGC 
GATGACCCAC TTGGTGAATG GGAAAGTTAC ATGCGATGGG TAGAAGAGAA TTTTCCTGAG 
AATAAAGAAT ACTTAACAAC TTTATTAGAG CATCTAATGA AGGAATTTTT AGATAAGAAG 
AGATATCACA ATGACCCAAG GTTCATCAAT TATTGTCTAC AATTTGCCAA GTGCAACAGT 
GACCTTCACC AGTTTTTTGA GTTTCTGCAC AACCACGGTA TCGGAACCCA GGCAGCTCCT 
CTGTACGTAG CCTGGGCCGG GCACCTGGAA GGCCAGGGGG AGTGGCAGCA CGCCGGTGCC 
GTTTTTCGGA GAGGAATTCA GAACCAGGCC GAACCTACAG AGCTGCTGCA ACAACAGTAC 
AGGATGTTTC AGACACGCCT CACTGAAACC CATTTGTCTA CTCAAGTGAG AACCTCAGAA 
CCTCTGCATG ATGCTCAGAT TTTAAATCAA ATGGTAACAT CAAACTCAAA TCCAGGAAGT 
AACTCAGCCT GCATTTCTAA GAATCAGGGT CCACAACTTC CTGGAGCAAC ACCTGCAGCT 
GGTGATAAAG GGGAACAAAG GGTGATCACA ATTTCTAAAT CAGAATATTC TTCACACGCA 
TCTGTGGCAG ACAAAGCTGA TGTTCAGCAG GTTGTCATGT ATTGCAAGGA GAAGCTTATT 
CGCGGAGAAT CGGAGTTTTC CTTCGAAGAG CTGAGAGCCC AGAGATACAA TCTACGGAGA 
AAGCATGAAC AATGGGTAAA TGAAGAGAGG CATTATATGA AAAGGAAAGA AGCAAATGCT 
TTGGAAGAAC AACTATTAAA ACAGAAAATG GATGAGCTTC ATAAGAAGCT GCACCAGGTG 
GTGGAGATGT CGCAGGGGAA CCTGCCCTCT TCCCGGGGGA CGTCTGAGGT CCGTCCAGCG 
TGTTTGGAGG CACACACAGA CTCCCAGCAG GAACTAAGAG CTCCGGGTCT TCCAGCCGTC 
CCTCAGCAGA CCTCGAAGAA CGCGGACGAG AAGCCCGGGG AGGCACCTTC TGTCCCTCTT 
ACGGCAAGTG CTGTCGCCAC TGCTTTGGTG TCCCCGGCCG CCAGCCAGGG TGTGGCTTGC 
CCGGCTCCTT TGGCAGGCCC GCCCGTGACG GACGCCATGT TTTCAGGGGG CGGCAGAGAT 
GCCAGGTGTG TGAACAAAAG TACTCATGAA TTCAGGCCAC AGAGCGGAGC AGAGATAAAA 
GAAGGGTGTG AAACACAGAA GGTTGTTAAC ACAAGTTCCT TTCACACAAC TCCAAACACA 
TCTCTGGGAC TGGTTCAGGC AACACCATCC AAAGTGCAGC CATCACCCAC GGTGCACACC 
AAGGAAGCTC TCGGTTTCAT CATGAATGTG TTTCAGGCTC CTACACTTCC TGATATTTCC 
GATGACAAAG AGGAATGGCC ATCTCTAGGT CAAAACGAGT TTGAAGCCCA GTTTCAAAAA 
AATGCACCAT CTTCTGGGGC TTGGGGAGTC AATAAGATCA TCTCTTCTTT GTCATCTGCT 
TTTCCTGTAT TTGAAGATGG AAACAAAGAA AATTATGGCT TACCACAGCC GAAAAATAAA 
CCTGCAGGAG CCAGGACCTT TGGAGAACGT TCCGTGAGCA GGTTTCCTGC AAAACCAAAT 
GAAGTGCCTC ATGCTGACGA GTTTCTGGAT GATTCAACCG TGTGGGGTGT CCGCTGCAAC 
AAAAGCCTGG CACCCAGCCC CAGGAGCCCG GGAGACTTCA GGTCCGCCGC GCAGCTGGCG 
TCTACGCCCT TCCACAGGCT TCCCGCGGAC TCCGCGCACC CACTGGAAGA TAAAGAAAAT 
ATGGTGGCGA ACCAGCACAC CCACGTGGCT GTGGATCCTT GTGCAAGAAA CAAGGTGGAA 
CCCTTGAAAG AGAGAAGGTT CAGTCCAGTC GAAGAGACAA GCCCAGACCC AGTGTGCCCC 
ACAGAGGGCC CTTCGGCGTC CTTGCTCCGT CTGGGCCAGC CTGCTGCAGC CGTACTGGTG 
AGCGAGGCAG AGCAGGGTCT CAAGGCCTGT AAAGTCACAG ACCCCGACTT GGCCACTGCA 
GAGGATCCGG CAGAAGCCAG CGCTGGGCTC CAGGCTGAAC TGATGCAGGC TGGCCCGCCT 
GGGACTGTCA ACGCTCCAAA CTTCACTGTT GAGAACCCCT GGGATGAGGA GTTGATTCTC 
AAACTTTTAT CCGGGCTTTC TAAACCAGTG AGTTCCTATC CAAATACTTA TGAATGGGCA 
TGTAAGCTTC CAGCCATCAA GCCCAAGACT GAATTCCAGT TAGGTTCTTT GCTGGTCTAC 
GTTGATCACT TTATTGGAGA AGGGGCTTTT GCCCAAGTCT ATGAAGTTAC CCATGGAGAT 
GTGAACAACA GCAAAAATAA GGAGAAATTT GCTTTGAAGG TCCAGAAGCC TCCCAACCTC 
TGGGAGTTCT ACATTGGGAC CCAGCTGATG GAGAGACTGA ACCCAGGTGC GCAGCACATG 
TTCATCAGGT TCTATTCGGC CCACCTGTTC CAGAACGGCA GCGTGTTGGT GGGGGACCTC 
TACAGCTACG GCACGCTGTT GAATGCCATT AATCTCTATA AAAATACCCC TGAAAAAGTG 
ATGCCTCAGG CACTAGTCCT CGCTTTTGCC ATCAGAATGC TCTGCATGAT CCAGCAAGTC 
CATGACTGTG AAATCATCCA CGGAGACATC AAGCCAGACA ATTTCATACT CGGAAACAGA 
TTTTTGGAAC AAGACAACAA AGATGATGAC TTATCTGCCG GCTTGGTCCT GATTGACCTG 
GGTCAGAGTA TAGACATGAA ACTTTTTCCG AAAGGAACTG CATTTACAGG GAAGTGTGAA 
ACTTCTGGTT TCCAGTGTAT TGAGATGCTC AGTAACAAGC CATGGAACTA CCAGATTGAT 
TACTTTGGAG TTGCTGCAAC CATATACTGC ATGCTTTGTG GCACTTACAT GAAAGTGAAA 
AATGAAGGGG GCATCTGGAA GCCTGAAGGT CTTTTTAGAA GGCTTCCTCA CCTGGACATG 
TGGAATGAGT TTTTTCACGT AATGTTGAAT ATACCCGATT GTCATCATCT TCCATCTTTG 
GATTTGTTAA GGCAGAAGCT AAAGAAAACA TTTCAAGAAC ACTACACCAG CAAGATCAAG 
ACCCTGTGTA ACAGGCTAAT TGTATTGCTC TTAGAATATA AGCGTTCTCG GAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012029288.1
CDS	529..3765
Translation	MDNPENVFQMFEAHMQSYKGDDPLGEWESYMRWVEENFPENKEYLTTLLEHLMKEFLDKKRYHNDPRFINYCLQFAKCNSDLHQFFEFLHNHGIGTQAAPLYVAWAGHLEGQGEWQHAGAVFRRGIQNQAEPTELLQQQYRMFQTRLTETHLSTQVRTSEPLHDAQILNQMVTSNSNPGSNSACISKNQGPQLPGATPAAGDKGEQRVITISKSEYSSHASVADKADVQQVVMYCKEKLIRGESEFSFEELRAQRYNLRRKHEQWVNEERHYMKRKEANALEEQLLKQKMDELHKKLHQVVEMSQGNLPSSRGTSEVRPACLEAHTDSQQELRAPGLPAVPQQTSKNADEKPGEAPSVPLTASAVATALVSPAASQGVACPAPLAGPPVTDAMFSGGGRDARCVNKSTHEFRPQSGAEIKEGCETQKVVNTSSFHTTPNTSLGLVQATPSKVQPSPTVHTKEALGFIMNVFQAPTLPDISDDKEEWPSLGQNEFEAQFQKNAPSSGAWGVNKIISSLSSAFPVFEDGNKENYGLPQPKNKPAGARTFGERSVSRFPAKPNEVPHADEFLDDSTVWGVRCNKSLAPSPRSPGDFRSAAQLASTPFHRLPADSAHPLEDKENMVANQHTHVAVDPCARNKVEPLKERRFSPVEETSPDPVCPTEGPSASLLRLGQPAAAVLVSEAEQGLKACKVTDPDLATAEDPAEASAGLQAELMQAGPPGTVNAPNFTVENPWDEELILKLLSGLSKPVSSYPNTYEWACKLPAIKPKTEFQLGSLLVYVDHFIGEGAFAQVYEVTHGDVNNSKNKEKFALKVQKPPNLWEFYIGTQLMERLNPGAQHMFIRFYSAHLFQNGSVLVGDLYSYGTLLNAINLYKNTPEKVMPQALVLAFAIRMLCMIQQVHDCEIIHGDIKPDNFILGNRFLEQDNKDDDLSAGLVLIDLGQSIDMKLFPKGTAFTGKCETSGFQCIEMLSNKPWNYQIDYFGVAATIYCMLCGTYMKVKNEGGIWKPEGLFRRLPHLDMWNEFFHVMLNIPDCHHLPSLDLLRQKLKKTFQEHYTSKIKTLCNRLIVLLLEYKRSRK

Target ORF information:

RefSeq Version	XM_012173898.2
Organism	Ovis aries(sheep)
Definition	Ovis aries BUB1, mitotic checkpoint serine/threonine kinase (BUB1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012173898.2

ORF Insert Sequence:

ATGGATAACC CGGAAAATGT GTTTCAAATG TTTGAAGCCC ATATGCAGAG CTACAAGGGC 
GATGACCCAC TTGGTGAATG GGAAAGTTAC ATGCGATGGG TAGAAGAGAA TTTTCCTGAG 
AATAAAGAAT ACTTAACAAC TTTATTAGAG CATCTAATGA AGGAATTTTT AGATAAGAAG 
AGATATCACA ATGACCCAAG GTTCATCAAT TATTGTCTAC AATTTGCCAA GTGCAACAGT 
GACCTTCACC AGTTTTTTGA GTTTCTGCAC AACCACGGTA TCGGAACCCA GGCAGCTCCT 
CTGTACGTAG CCTGGGCCGG GCACCTGGAA GGCCAGGGGG AGTGGCAGCA CGCCGGTGCC 
GTTTTTCGGA GAGGAATTCA GAACCAGGCC GAACCTACAG AGCTGCTGCA ACAACAGTAC 
AGGATGTTTC AGACACGCCT CACTGAAACC CATTTGTCTA CTCAAGTGAG AACCTCAGAA 
CCTCTGCATG ATGCTCAGAT TTTAAATCAA ATGGTAACAT CAAACTCAAA TCCAGGAAGT 
AACTCAGCCT GCATTTCTAA GAATCAGGGT CCACAACTTC CTGGAGCAAC ACCTGCAGCT 
GGTGATAAAG GGGAACAAAG GGTGATCACA ATTTCTAAAT CAGAATATTC TTCACACGCA 
TCTGTGGCAG ACAAAGCTGA TGTTCAGCAG GTTGTCATGT ATTGCAAGGA GAAGCTTATT 
CGCGGAGAAT CGGAGTTTTC CTTCGAAGAG CTGAGAGCCC AGAGATACAA TCTACGGAGA 
AAGCATGAAC AATGGGTAAA TGAAGAGAGG CATTATATGA AAAGGAAAGA AGCAAATGCT 
TTGGAAGAAC AACTATTAAA ACAGAAAATG GATGAGCTTC ATAAGAAGCT GCACCAGGTG 
GTGGAGATGT CGCAGGGGAA CCTGCCCTCT TCCCGGGGGA CGTCTGAGGT CCGTCCAGCG 
TGTTTGGAGG CACACACAGA CTCCCAGCAG GAACTAAGAG CTCCGGGTCT TCCAGCCGTC 
CCTCAGCAGA CCTCGAAGAA CGCGGACGAG AAGCCCGGGG AGGCACCTTC TGTCCCTCTT 
ACGGCAAGTG CTGTCGCCAC TGCTTTGGTG TCCCCGGCCG CCAGCCAGGG TGTGGCTTGC 
CCGGCTCCTT TGGCAGGCCC GCCCGTGACG GACGCCATGT TTTCAGGGGG CGGCAGAGAT 
GCCAGGTGTG TGAACAAAAG TACTCATGAA TTCAGGCCAC AGAGCGGAGC AGAGATAAAA 
GAAGGGTGTG AAACACAGAA GGTTGTTAAC ACAAGTTCCT TTCACACAAC TCCAAACACA 
TCTCTGGGAC TGGTTCAGGC AACACCATCC AAAGTGCAGC CATCACCCAC GGTGCACACC 
AAGGAAGCTC TCGGTTTCAT CATGAATGTG TTTCAGGCTC CTACACTTCC TGATATTTCC 
GATGACAAAG AGGAATGGCC ATCTCTAGGT CAAAACGAGT TTGAAGCCCA GTTTCAAAAA 
AATGCACCAT CTTCTGGGGC TTGGGGAGTC AATAAGATCA TCTCTTCTTT GTCATCTGCT 
TTTCCTGTAT TTGAAGATGG AAACAAAGAA AATTATGGCT TACCACAGCC GAAAAATAAA 
CCTGCAGGAG CCAGGACCTT TGGAGAACGT TCCGTGAGCA GGTTTCCTGC AAAACCAAAT 
GAAGTGCCTC ATGCTGACGA GTTTCTGGAT GATTCAACCG TGTGGGGTGT CCGCTGCAAC 
AAAAGCCTGG CACCCAGCCC CAGGAGCCCG GGAGACTTCA GGTCCGCCGC GCAGCTGGCG 
TCTACGCCCT TCCACAGGCT TCCCGCGGAC TCCGCGCACC CACTGGAAGA TAAAGAAAAT 
ATGGTGGCGA ACCAGCACAC CCACGTGGCT GTGGATCCTT GTGCAAGAAA CAAGGTGGAA 
CCCTTGAAAG AGAGAAGGTT CAGTCCAGTC GAAGAGACAA GCCCAGACCC AGTGTGCCCC 
ACAGAGGGCC CTTCGGCGTC CTTGCTCCGT CTGGGCCAGC CTGCTGCAGC CGTACTGGTG 
AGCGAGGCAG AGCAGGGTCT CAAGGCCTGT AAAGTCACAG ACCCCGACTT GGCCACTGCA 
GAGGATCCGG CAGAAGCCAG CGCTGGGCTC CAGGCTGAAC TGATGCAGGC TGGCCCGCCT 
GGGACTGTCA ACGCTCCAAA CTTCACTGTT GAGAACCCCT GGGATGAGGA GTTGATTCTC 
AAACTTTTAT CCGGGCTTTC TAAACCAGTG AGTTCCTATC CAAATACTTA TGAATGGGCA 
TGTAAGCTTC CAGCCATCAA GCCCAAGACT GAATTCCAGT TAGGTTCTTT GCTGGTCTAC 
GTTGATCACT TTATTGGAGA AGGGGCTTTT GCCCAAGTCT ATGAAGTTAC CCATGGAGAT 
GTGAACAACA GCAAAAATAA GGAGAAATTT GCTTTGAAGG TCCAGAAGCC TCCCAACCTC 
TGGGAGTTCT ACATTGGGAC CCAGCTGATG GAGAGACTGA ACCCAGGTGC GCAGCACATG 
TTCATCAGGT TCTATTCGGC CCACCTGTTC CAGAACGGCA GCGTGTTGGT GGGGGACCTC 
TACAGCTACG GCACGCTGTT GAATGCCATT AATCTCTATA AAAATACCCC TGAAAAAGTG 
ATGCCTCAGG CACTAGTCCT CGCTTTTGCC ATCAGAATGC TCTGCATGAT CCAGCAAGTC 
CATGACTGTG AAATCATCCA CGGAGACATC AAGCCAGACA ATTTCATACT CGGAAACAGA 
TTTTTGGAAC AAGACAACAA AGATGATGAC TTATCTGCCG GCTTGGTCCT GATTGACCTG 
GGTCAGAGTA TAGACATGAA ACTTTTTCCG AAAGGAACTG CATTTACAGG GAAGTGTGAA 
ACTTCTGGTT TCCAGTGTAT TGAGATGCTC AGTAACAAGC CATGGAACTA CCAGATTGAT 
TACTTTGGAG TTGCTGCAAC CATATACTGC ATGCTTTGTG GCACTTACAT GAAAGTGAAA 
AATGAAGGGG GCATCTGGAA GCCTGAAGGT CTTTTTAGAA GGCTTCCTCA CCTGGACATG 
TGGAATGAGT TTTTTCACGT AATGTTGAAT ATACCCGATT GTCATCATCT TCCATCTTTG 
GATTTGTTAA GGCAGAAGCT AAAGAAAACA TTTCAAGAAC ACTACACCAG CAAGATCAAG 
ACCCTGTGTA ACAGGCTAAT TGTATTGCTC TTAGAATATA AGCGTTCTCG GAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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BUB1 cDNA ORF clone, Ovis aries(sheep)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Eメール

電話

FAX

オンラインフォーム

サポート

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