ADAMTS20 cDNA ORF clone, Ovis aries musimon(Ovis orientalis musimon)

Gene
Clones
gRNAs

Summary

Gene Symbol	ADAMTS20
Entrez Gene ID	101115444
Full Name	ADAM metallopeptidase with thrombospondin type 1 motif 20
Gene Type	protein-coding
Organism	Ovis aries musimon(Ovis orientalis musimon)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015094726.2	XP_014950212.2	A disintegrin and metalloproteinase with thrombospondin motifs 20 isoform X2
XM_012174462.2	XP_012029852.1	A disintegrin and metalloproteinase with thrombospondin motifs 20 isoform X2
XM_012162371.2	XP_012017761.1	A disintegrin and metalloproteinase with thrombospondin motifs 20 isoform X1
XM_015094726.1	XP_014950212.1	A disintegrin and metalloproteinase with thrombospondin motifs 20 isoform X2
XM_004006435.3	XP_004006484.1	A disintegrin and metalloproteinase with thrombospondin motifs 20 isoform X1
XM_012162373.2	XP_012017763.1	A disintegrin and metalloproteinase with thrombospondin motifs 20 isoform X2
XM_004006435.4	XP_004006484.2	A disintegrin and metalloproteinase with thrombospondin motifs 20 isoform X1
XM_015089910.1	XP_014945396.1	A disintegrin and metalloproteinase with thrombospondin motifs 20 isoform X2

Pathways from BioSystems
Gene Ontology

The following ADAMTS20 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ADAMTS20 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OOv33872	XM_012162371.2 Latest version!	Ovis aries musimon ADAM metallopeptidase with thrombospondin type 1 motif 20 (ADAMTS20), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OOv33873	XM_015089910.1 Latest version!	Ovis aries musimon ADAM metallopeptidase with thrombospondin type 1 motif 20 (ADAMTS20), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OOv33873	XM_012162373.2 Latest version!	Ovis aries musimon ADAM metallopeptidase with thrombospondin type 1 motif 20 (ADAMTS20), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OSm77935	XM_004006435.4 Latest version!	Ovis aries ADAM metallopeptidase with thrombospondin type 1 motif 20 (ADAMTS20), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSm77936	XM_015094726.2 Latest version!	Ovis aries ADAM metallopeptidase with thrombospondin type 1 motif 20 (ADAMTS20), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OSm04356	XM_004006435.3 Latest version!	Ovis aries ADAM metallopeptidase with thrombospondin type 1 motif 20 (ADAMTS20), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OSm36428	XM_012174462.2 Latest version!	Ovis aries ADAM metallopeptidase with thrombospondin type 1 motif 20 (ADAMTS20), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OSm36428	XM_015094726.1 Latest version!	Ovis aries ADAM metallopeptidase with thrombospondin type 1 motif 20 (ADAMTS20), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ADAMTS20 ( XM_012162371.2 ) cDNA ORF clone, Ovis aries musimon(Ovis orientalis musimon) -> XP_012017761.1 Ovis aries musimon ADAM metallopeptidase with thrombospondin type 1 motif 20 (ADAMTS20), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OOv33872
Clone ID Related Accession (Same CDS sequence)	XM_012162371.2
Accession Version	XM_012162371.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5748bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	A disintegrin and metalloproteinase with thrombospondin motifs 20 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942999.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012162371.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGGTGG TCAAGTGGCT GACGGGGCTG CTCTACCAGC TCTCGCTCTT CATCACCAGG 
TCTTGGGAAG TTCACTTCCA CCCCAGGCAG GAAGCCTTAG TGAGGACACT GGCCTCCTAC 
GAAGTGGTGA CCCCCGCGCG GGTCAATGAG TTCGGCGAAG TATTCCCGCA GAGCCACCAC 
TTCAGCCGGA GGAAGCGCAG CTCGGAGACT CTGGAGCCCA CACCGTTCAG AACCCACTAC 
CGAATCCGCG CCTACGGGCA GCTCTTCCAA CTGAACCTGA GCGCCGACGC GGCCTTCCTG 
GCTGCGGGAT ACACCGAGGT GCACTTAGGC ACCTCTGCGC ACCCGGCGGA GGAGCGCAGC 
GCGGCGCCCG CAGACCTGCG CCACTGCTTC TACCGCGGCC AGGTCAATGC TCGCGAGGAT 
CACACAGCCG TCTTCAGCCT CTGTGGAGGC TTGATGGGAA CATTTAAAGC ACATGATGGT 
GAATATTTCT TAGAACCTAT AATGAAGGCA GATGGGAGTG AACATGAAGA TGATCATAGC 
AAGCCACATC TTATATACAG ACAGGAATTA AAGGGGAACT ATTTTCTGCA GTCTCACAAG 
CCTTGTGATG CTTCAGAAAG TGAAATAAAG AAAACTGCTC TACCCTTTCA TAACTACAGC 
GACATTAATG AAAATCTCAA TATAAAGGAA GAAAGAGTTT TAGGATACAC TTCAAAAAAT 
ATATCTTTGG AAGATGAAAG AAGTCAATTG CATTCCAGGA AGAAACGTTT TTTATCATAT 
CCAAGATACG TGGAAATTAT GGTTACAGCT GACTCTAAAA TGGTTCATCA TCATGGACAG 
AACTTGCAGC ACTACGTACT AACTCTGATG TCAATTGTTG CAGCAATCTA CAAGGACTCA 
AGTATTGGAA ATCTTATAAA CATAGTAATT GTAAAATTAG TTATAATTCA TGATGAGCAG 
GAAGGACCAG TCATCAGTTT TAATGCAGCT ACCACGTTAC GCAACTTCTG TTTGTGGCAA 
CAAACTCAGA ATGTTCTTGA CGATGCCCAC CCATCCCACC ATGATACTGC TGTTCTTATC 
ACAAGGGAAG ACATTTGTGG AGCTAGAGAG AAATGTGACA CATTAGGTTT AGCAGAATTA 
GGTACCCTGT GTGACCCTTT ACGGAGCTGC TCTATTAGTG AAGAAAATGG GCTCAGTGCT 
GCCTTTACCA TAGCCCATGA GCTTGGTCAT GTATTTAATG TTCCACACGA TGATAGTTTT 
AAATGTAAGG AAACTGGAAT TAAACATCAG TATCATGTCA TGGCTCCAAC TTTAAATTAC 
CACACAAGTC CTTGGACCTG GTCAAAATGT AGTCAGAAAT ATATCACTGA ATTCCTAGAC 
ACCGGTCATG GGGAATGCCT CCTTGACAAA CCAAATGGAA GAATATACGA TTTGTCTTCA 
CAGCTTCCTG GATTAATGTA TGATGTAAAC AAGCAATGTG AGCTTATGTT TGGTCCTGGA 
TCACAAGTGT GTCCCTATTT AAAACAATGC AGGCGTCTCT GGTGTACCAG CGCAGAAGGA 
GTCCACAAGG GCTGTCGCAC TCAACACATG CCTCTGGCAG ACGGAACAAA CTGTGGTCCT 
GGGATGCATT GCCACCATGG GCTATGTGTA AATAAAGAAA TGGAGACACG TCCTGTAGAT 
GGAGATTGGG GACCATGGGG ACCTTACAGT TCTTGTTCAA GAACATGTGG AGGTGGAATC 
AAAAGCACAA GCAGGCTCTG TAATCGCCCC GAGCCAAGAA ACGGAGGCAA GTACTGTGTG 
GGCCGCAGGA TGAAATTTCG ATCATGTAAC ACTGATTCAT GTCCAAAAGG CAAGCAAGAC 
TTCCGTGAGA AGCAGTGCTC TGATTTTGAT GGTAAACATT TCAACATCAA TGGTCTCTCC 
CCTAACGTGA GGTGGCTTCC AAAGTACAGT GGGATTGCTG TAAAAGATCG TTGTAAACTC 
TATTGTCGGG TGGCTGGAAC CACTAGCTTC TACCAACTGA AGGACAGGGT TGCCGATGGT 
ACTCCTTGTG GAACGGAAAC AAATGACATC TGTGTTCAAG GCCTGTGTCG GCAAGCTGGC 
TGTGATCACG TGTTAAACTC CAAGGCCAGG AGAGACAAAT GTGGCGTGTG TGGTGGGGAT 
AACTCCTCCT GCAGGACAAT GGCAGGCGTC TTCAACAGTG CGCATTATGG TTATAATGTC 
GTTGTAAAGA TTCCCCCAGG AGCAACAAAC ATTGATATTC TTCAGCACAG CTATTCTGGA 
AAACCAGAAG ATGACAATTA CCTGGCATTA TCGGACACTC AAGGGAATTT TCTTTTGAAT 
GGGAATTTTG TTGTAAGTAT GTCCAAACGG GAAATCAACG TACAAGGAGC TATTTTTGAA 
TACAGTGGTT CAAACAACTC AATTGAAAGG ATTAATAGTA CTGATCGACT GGAAGAAGAA 
CTTGTTTTGC AGGTTTTGTG TGTGGGTAAT CTATACAACC CTGATGTACG TTATTCCTTC 
AATATCCCCG TGGAAGAGAA GAGTGATCTA TTCACATGGG ACCCGTATGG ACCATGGCAA 
GACTGTACAA AAATGTGTCA AGGTCTTCAT AGAAGAAAAA TTACTTGTGT ACGTAAGAGT 
GATCATATGG TCATGTCTGA TCAAAGCTGT GACCACTTAC CACTTCCATT GCTGGTAACT 
GAAAGGTGCA ATACAGACTG TGAACTCAGG TGGCACATCA TTGGCAAAAG TGAATGTTCA 
ACCCTTTGTG GTCAAGGATA CAAGTCATTG GACGTCCATT GTATGAAGTA TTCCATTCAT 
AAAGGACAGA CTGTTCCAGT AGATGACCAC TACTGCAGTG ACCAACTTAA ACCTCCAACC 
CGAGAACCCT GCCATGGTGA CTGTGTCTTA ACAAGGTGGC ATTATTTAGA ATGGTCTCAG 
TGTTCCAGGA GTTGCGGAGG AGGGGAGAGG TCTCGAGAAT CTTACTGTAT AAATAACTTT 
GGCCACCGTC TTGCTGACAG AGAATGCCAG GAACTTCCCC GAGTGACAAC AGAGAATTGC 
AATGAATTTT CCTGTCCCAG TTGGGCCACT AGTGAGTGGA GTGAGTGTCT GGTTACCTGT 
GGAAAAGGAA CAAAGCAGCG GCAGGTGTGG TGTCACCTGA ATGAAGATCA CTTGAGTGAT 
GGCTTCTGTG ACCCAAGCAC CAAGCCTGAG TCTCAGAGAC CCTGTGAGCT TCATGCATGT 
ACTTCCTGGC AAGTAGGACC TTGGGGTTCT TGCACAGCCA CGTGTGGACA GGGGTACCAG 
ATGCGTGCTG TGAAGTGTGT CAACGAGCTA TTTAGCACAG TGCTAGATGA CAGACTGTGC 
CATGGAGCTA GTCGCCCAAG TGAAAGGCAG AATTGTATTG TTACACCTTG CCCAGTTATC 
CCTAAAATTG GGGCCACTTC CTTGCCAGCT GTTCCCATGA GAAAGATAAC ACAATGGCGA 
TATGGTTCTT GGACACCATG CTCCGTGTCT TGTGGAAGAG GAAATCAAGC CCGCTATGTA 
AGTTGTCGTG ATGCTCATGA TGGAATAGCA GATGAATCAT TTTGTGCCCA CTTACCCCGA 
CCGGCTGAAA TAGCTGTCTG TTTTAGCCCT TGTGGAGAGT GGCAAACAGG GAATTGGTCA 
CCTTGTTCAG CTTCCTGTGG CCGTGGAAAA ATGACTCGAC AAGTTTTATG CATCAACTAT 
CATCAGCCAG TTAATGAGAA TTACTGTGAC CCTGAAGTCC GCCCTGTGAT AGAACAAGAA 
TGTAATCTGG CAGCCTGCCC TCCCACGTAC AGCCACTTTC CGAGTTCCTC TGAGCAGCCA 
AGCCATTTTC CAGGCAGGAA TTTTCCATTA ACTCACAAAC CAGAAGATAA TCAAAATCAG 
AGGGTCCATC CATCAATCAG AGAAAACCAG TGGAGAACAG GACCATGGGG ATCATGCTCT 
AGCAGTTGTG CTGGAGGTGT TCAGCGTAGA GTTGTGGTCT GCCAGGATGA AAATGGACAA 
AGTGCCAGTT ACTGTGATGC AGCGTCCAAA CCCCCAGAGT CAAAGCACTG TGATTCAGGA 
CCTTGTCCAC AGTGGAACTA TGGAAGCTGG GGAGAATGTA CGCAAACGTG TGGAGGAGGA 
ATAAAATCAA GATTTGTAAT ATGCCAGTTT CCCGATGGCC AGCTGTCACA AGAGCAAAAC 
TGTGAAATGT TGAACAAGCC GCCTAGTGTG GTACAGTGTC ATATACATGC TTGCCCTGAT 
GACGTATCAT GGCATCGGGG ACCATGGAAA TCGTGCTCAG CTTCCTGTGG TAAAGGTTTA 
AAATACCGTG AGGTGCTTTG CATTGACCAA TTCCACAGGA AATTAGAAGA AAAATATTGC 
AGTCATCTAC AGAAACCTCG AACACATAAA GCTTGTAGAT CTGTCCGATG CCCTTCATGG 
AAAGCCAACA GATGGAAGGA GTGCTCTGTG ACCTGCGGCT CCGGAGTTCA GCGGAGGGAT 
GTGTACTGCA GACTCAGAGG AGTTGGTCGG GTGGCGGAAG AAAAGTGTGA TCCATCCACG 
CGGCCTTATT TTCAGAGAGA GTGTTGGCAT CGGGACTGCA TACAGTACCA CTGGGCGGCA 
GGAGAGTGGT TGGATTGCTC AGTATCGTGT AAGAAAAGAG AGACATATCG GCAAGTGAAG 
TGCATGGATG CACAAAACAT TCAAGTGAGT GAGAGTTTCT GTGACCTCGC GACCAGACCT 
CTCGCGGTGA AAAAGTGCAG AAACTCTCCC TGCAAGTATA TCGTGGTAAC AGGAGACTCA 
TCCCAGTGTG CAGGTAACTG TGGATTTAGT TATCGACAAA GGATTACATA TTGCGTTGGG 
GTCCAATCTA TGGAGAAACA TAAACTTCAT GAGCTGTGGC CTATAGACTA TCGAGTATGC 
CCTGTGCTGC CTTCCCCTCA GGTTTACAAA TGCAATTTTA GGACCTGTTT GCATATGGCC 
ACTTGGAAAG TTGGAAAATG GAGCAAGTGC TCTGTGACTT GTGGAGTTGG GATGATGGAG 
AGAAGAGTGG AATGCATGGC CGATAATGGT CTGTCCAGTG ATTTATGTTT AAAGCATTTA 
AAACCAGATG CTCAAAAGAA ATGTTATGTC AGTGACTGTA AAACATTTAT GAGCTGTAAA 
GAAATACAAG TGAAAAACAA CATTACCAAG GATGGTGACT ATTACCTTAA CATTAAAGGA 
AGAATAACAA AGATCTATTG TGCTGGCATG CACTTGGAGA ACCCTAAGGA ATATATATCA 
TTGGTCAAAG GTGAAGAAGA CAACTTTTCT GAAGTGTATG GTTTTAGACT ACGAAATCCA 
TATGAATGTC CTTTTAATGG AAGCAGGAGG CATGACTGTG AATGTAAAAA TGACTATCTG 
GCTGCTGGAC ACACTGTTTT TAGCAAAATA AGAATTGATC TTATTTCCAT GCAAATTAAA 
ACTACAGACC TTCTTTTTGC CCACACAATA TTTGGAAATG CAGTTCCATT TGCCACAGCT 
GGAGATTGCT ATAGTGCTGC CAGATGCCCA CAGGGACAGT TCAGCATTAA TTTGGCAGGA 
ACTGGGATGA AGATATCCAG TACAGCAAAG TGGCTGGCTC AGGGCAGTTA TGCCTCTGTG 
ATCATTCACA GATCACAGGA TGGAACCAAA GTCTATGGCA GATGTGGAGG GTTCTGTGGA 
AAGTGTATTC CTCACATGAC TACTGGTCTC CCAATTCAAG TAGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012017761.1
CDS	333..6080
Translation	MRVVKWLTGLLYQLSLFITRSWEVHFHPRQEALVRTLASYEVVTPARVNEFGEVFPQSHHFSRRKRSSETLEPTPFRTHYRIRAYGQLFQLNLSADAAFLAAGYTEVHLGTSAHPAEERSAAPADLRHCFYRGQVNAREDHTAVFSLCGGLMGTFKAHDGEYFLEPIMKADGSEHEDDHSKPHLIYRQELKGNYFLQSHKPCDASESEIKKTALPFHNYSDINENLNIKEERVLGYTSKNISLEDERSQLHSRKKRFLSYPRYVEIMVTADSKMVHHHGQNLQHYVLTLMSIVAAIYKDSSIGNLINIVIVKLVIIHDEQEGPVISFNAATTLRNFCLWQQTQNVLDDAHPSHHDTAVLITREDICGAREKCDTLGLAELGTLCDPLRSCSISEENGLSAAFTIAHELGHVFNVPHDDSFKCKETGIKHQYHVMAPTLNYHTSPWTWSKCSQKYITEFLDTGHGECLLDKPNGRIYDLSSQLPGLMYDVNKQCELMFGPGSQVCPYLKQCRRLWCTSAEGVHKGCRTQHMPLADGTNCGPGMHCHHGLCVNKEMETRPVDGDWGPWGPYSSCSRTCGGGIKSTSRLCNRPEPRNGGKYCVGRRMKFRSCNTDSCPKGKQDFREKQCSDFDGKHFNINGLSPNVRWLPKYSGIAVKDRCKLYCRVAGTTSFYQLKDRVADGTPCGTETNDICVQGLCRQAGCDHVLNSKARRDKCGVCGGDNSSCRTMAGVFNSAHYGYNVVVKIPPGATNIDILQHSYSGKPEDDNYLALSDTQGNFLLNGNFVVSMSKREINVQGAIFEYSGSNNSIERINSTDRLEEELVLQVLCVGNLYNPDVRYSFNIPVEEKSDLFTWDPYGPWQDCTKMCQGLHRRKITCVRKSDHMVMSDQSCDHLPLPLLVTERCNTDCELRWHIIGKSECSTLCGQGYKSLDVHCMKYSIHKGQTVPVDDHYCSDQLKPPTREPCHGDCVLTRWHYLEWSQCSRSCGGGERSRESYCINNFGHRLADRECQELPRVTTENCNEFSCPSWATSEWSECLVTCGKGTKQRQVWCHLNEDHLSDGFCDPSTKPESQRPCELHACTSWQVGPWGSCTATCGQGYQMRAVKCVNELFSTVLDDRLCHGASRPSERQNCIVTPCPVIPKIGATSLPAVPMRKITQWRYGSWTPCSVSCGRGNQARYVSCRDAHDGIADESFCAHLPRPAEIAVCFSPCGEWQTGNWSPCSASCGRGKMTRQVLCINYHQPVNENYCDPEVRPVIEQECNLAACPPTYSHFPSSSEQPSHFPGRNFPLTHKPEDNQNQRVHPSIRENQWRTGPWGSCSSSCAGGVQRRVVVCQDENGQSASYCDAASKPPESKHCDSGPCPQWNYGSWGECTQTCGGGIKSRFVICQFPDGQLSQEQNCEMLNKPPSVVQCHIHACPDDVSWHRGPWKSCSASCGKGLKYREVLCIDQFHRKLEEKYCSHLQKPRTHKACRSVRCPSWKANRWKECSVTCGSGVQRRDVYCRLRGVGRVAEEKCDPSTRPYFQRECWHRDCIQYHWAAGEWLDCSVSCKKRETYRQVKCMDAQNIQVSESFCDLATRPLAVKKCRNSPCKYIVVTGDSSQCAGNCGFSYRQRITYCVGVQSMEKHKLHELWPIDYRVCPVLPSPQVYKCNFRTCLHMATWKVGKWSKCSVTCGVGMMERRVECMADNGLSSDLCLKHLKPDAQKKCYVSDCKTFMSCKEIQVKNNITKDGDYYLNIKGRITKIYCAGMHLENPKEYISLVKGEEDNFSEVYGFRLRNPYECPFNGSRRHDCECKNDYLAAGHTVFSKIRIDLISMQIKTTDLLFAHTIFGNAVPFATAGDCYSAARCPQGQFSINLAGTGMKISSTAKWLAQGSYASVIIHRSQDGTKVYGRCGGFCGKCIPHMTTGLPIQVV

Target ORF information:

RefSeq Version	XM_012162371.2
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon ADAM metallopeptidase with thrombospondin type 1 motif 20 (ADAMTS20), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012162371.2

ORF Insert Sequence:

ATGCGGGTGG TCAAGTGGCT GACGGGGCTG CTCTACCAGC TCTCGCTCTT CATCACCAGG 
TCTTGGGAAG TTCACTTCCA CCCCAGGCAG GAAGCCTTAG TGAGGACACT GGCCTCCTAC 
GAAGTGGTGA CCCCCGCGCG GGTCAATGAG TTCGGCGAAG TATTCCCGCA GAGCCACCAC 
TTCAGCCGGA GGAAGCGCAG CTCGGAGACT CTGGAGCCCA CACCGTTCAG AACCCACTAC 
CGAATCCGCG CCTACGGGCA GCTCTTCCAA CTGAACCTGA GCGCCGACGC GGCCTTCCTG 
GCTGCGGGAT ACACCGAGGT GCACTTAGGC ACCTCTGCGC ACCCGGCGGA GGAGCGCAGC 
GCGGCGCCCG CAGACCTGCG CCACTGCTTC TACCGCGGCC AGGTCAATGC TCGCGAGGAT 
CACACAGCCG TCTTCAGCCT CTGTGGAGGC TTGATGGGAA CATTTAAAGC ACATGATGGT 
GAATATTTCT TAGAACCTAT AATGAAGGCA GATGGGAGTG AACATGAAGA TGATCATAGC 
AAGCCACATC TTATATACAG ACAGGAATTA AAGGGGAACT ATTTTCTGCA GTCTCACAAG 
CCTTGTGATG CTTCAGAAAG TGAAATAAAG AAAACTGCTC TACCCTTTCA TAACTACAGC 
GACATTAATG AAAATCTCAA TATAAAGGAA GAAAGAGTTT TAGGATACAC TTCAAAAAAT 
ATATCTTTGG AAGATGAAAG AAGTCAATTG CATTCCAGGA AGAAACGTTT TTTATCATAT 
CCAAGATACG TGGAAATTAT GGTTACAGCT GACTCTAAAA TGGTTCATCA TCATGGACAG 
AACTTGCAGC ACTACGTACT AACTCTGATG TCAATTGTTG CAGCAATCTA CAAGGACTCA 
AGTATTGGAA ATCTTATAAA CATAGTAATT GTAAAATTAG TTATAATTCA TGATGAGCAG 
GAAGGACCAG TCATCAGTTT TAATGCAGCT ACCACGTTAC GCAACTTCTG TTTGTGGCAA 
CAAACTCAGA ATGTTCTTGA CGATGCCCAC CCATCCCACC ATGATACTGC TGTTCTTATC 
ACAAGGGAAG ACATTTGTGG AGCTAGAGAG AAATGTGACA CATTAGGTTT AGCAGAATTA 
GGTACCCTGT GTGACCCTTT ACGGAGCTGC TCTATTAGTG AAGAAAATGG GCTCAGTGCT 
GCCTTTACCA TAGCCCATGA GCTTGGTCAT GTATTTAATG TTCCACACGA TGATAGTTTT 
AAATGTAAGG AAACTGGAAT TAAACATCAG TATCATGTCA TGGCTCCAAC TTTAAATTAC 
CACACAAGTC CTTGGACCTG GTCAAAATGT AGTCAGAAAT ATATCACTGA ATTCCTAGAC 
ACCGGTCATG GGGAATGCCT CCTTGACAAA CCAAATGGAA GAATATACGA TTTGTCTTCA 
CAGCTTCCTG GATTAATGTA TGATGTAAAC AAGCAATGTG AGCTTATGTT TGGTCCTGGA 
TCACAAGTGT GTCCCTATTT AAAACAATGC AGGCGTCTCT GGTGTACCAG CGCAGAAGGA 
GTCCACAAGG GCTGTCGCAC TCAACACATG CCTCTGGCAG ACGGAACAAA CTGTGGTCCT 
GGGATGCATT GCCACCATGG GCTATGTGTA AATAAAGAAA TGGAGACACG TCCTGTAGAT 
GGAGATTGGG GACCATGGGG ACCTTACAGT TCTTGTTCAA GAACATGTGG AGGTGGAATC 
AAAAGCACAA GCAGGCTCTG TAATCGCCCC GAGCCAAGAA ACGGAGGCAA GTACTGTGTG 
GGCCGCAGGA TGAAATTTCG ATCATGTAAC ACTGATTCAT GTCCAAAAGG CAAGCAAGAC 
TTCCGTGAGA AGCAGTGCTC TGATTTTGAT GGTAAACATT TCAACATCAA TGGTCTCTCC 
CCTAACGTGA GGTGGCTTCC AAAGTACAGT GGGATTGCTG TAAAAGATCG TTGTAAACTC 
TATTGTCGGG TGGCTGGAAC CACTAGCTTC TACCAACTGA AGGACAGGGT TGCCGATGGT 
ACTCCTTGTG GAACGGAAAC AAATGACATC TGTGTTCAAG GCCTGTGTCG GCAAGCTGGC 
TGTGATCACG TGTTAAACTC CAAGGCCAGG AGAGACAAAT GTGGCGTGTG TGGTGGGGAT 
AACTCCTCCT GCAGGACAAT GGCAGGCGTC TTCAACAGTG CGCATTATGG TTATAATGTC 
GTTGTAAAGA TTCCCCCAGG AGCAACAAAC ATTGATATTC TTCAGCACAG CTATTCTGGA 
AAACCAGAAG ATGACAATTA CCTGGCATTA TCGGACACTC AAGGGAATTT TCTTTTGAAT 
GGGAATTTTG TTGTAAGTAT GTCCAAACGG GAAATCAACG TACAAGGAGC TATTTTTGAA 
TACAGTGGTT CAAACAACTC AATTGAAAGG ATTAATAGTA CTGATCGACT GGAAGAAGAA 
CTTGTTTTGC AGGTTTTGTG TGTGGGTAAT CTATACAACC CTGATGTACG TTATTCCTTC 
AATATCCCCG TGGAAGAGAA GAGTGATCTA TTCACATGGG ACCCGTATGG ACCATGGCAA 
GACTGTACAA AAATGTGTCA AGGTCTTCAT AGAAGAAAAA TTACTTGTGT ACGTAAGAGT 
GATCATATGG TCATGTCTGA TCAAAGCTGT GACCACTTAC CACTTCCATT GCTGGTAACT 
GAAAGGTGCA ATACAGACTG TGAACTCAGG TGGCACATCA TTGGCAAAAG TGAATGTTCA 
ACCCTTTGTG GTCAAGGATA CAAGTCATTG GACGTCCATT GTATGAAGTA TTCCATTCAT 
AAAGGACAGA CTGTTCCAGT AGATGACCAC TACTGCAGTG ACCAACTTAA ACCTCCAACC 
CGAGAACCCT GCCATGGTGA CTGTGTCTTA ACAAGGTGGC ATTATTTAGA ATGGTCTCAG 
TGTTCCAGGA GTTGCGGAGG AGGGGAGAGG TCTCGAGAAT CTTACTGTAT AAATAACTTT 
GGCCACCGTC TTGCTGACAG AGAATGCCAG GAACTTCCCC GAGTGACAAC AGAGAATTGC 
AATGAATTTT CCTGTCCCAG TTGGGCCACT AGTGAGTGGA GTGAGTGTCT GGTTACCTGT 
GGAAAAGGAA CAAAGCAGCG GCAGGTGTGG TGTCACCTGA ATGAAGATCA CTTGAGTGAT 
GGCTTCTGTG ACCCAAGCAC CAAGCCTGAG TCTCAGAGAC CCTGTGAGCT TCATGCATGT 
ACTTCCTGGC AAGTAGGACC TTGGGGTTCT TGCACAGCCA CGTGTGGACA GGGGTACCAG 
ATGCGTGCTG TGAAGTGTGT CAACGAGCTA TTTAGCACAG TGCTAGATGA CAGACTGTGC 
CATGGAGCTA GTCGCCCAAG TGAAAGGCAG AATTGTATTG TTACACCTTG CCCAGTTATC 
CCTAAAATTG GGGCCACTTC CTTGCCAGCT GTTCCCATGA GAAAGATAAC ACAATGGCGA 
TATGGTTCTT GGACACCATG CTCCGTGTCT TGTGGAAGAG GAAATCAAGC CCGCTATGTA 
AGTTGTCGTG ATGCTCATGA TGGAATAGCA GATGAATCAT TTTGTGCCCA CTTACCCCGA 
CCGGCTGAAA TAGCTGTCTG TTTTAGCCCT TGTGGAGAGT GGCAAACAGG GAATTGGTCA 
CCTTGTTCAG CTTCCTGTGG CCGTGGAAAA ATGACTCGAC AAGTTTTATG CATCAACTAT 
CATCAGCCAG TTAATGAGAA TTACTGTGAC CCTGAAGTCC GCCCTGTGAT AGAACAAGAA 
TGTAATCTGG CAGCCTGCCC TCCCACGTAC AGCCACTTTC CGAGTTCCTC TGAGCAGCCA 
AGCCATTTTC CAGGCAGGAA TTTTCCATTA ACTCACAAAC CAGAAGATAA TCAAAATCAG 
AGGGTCCATC CATCAATCAG AGAAAACCAG TGGAGAACAG GACCATGGGG ATCATGCTCT 
AGCAGTTGTG CTGGAGGTGT TCAGCGTAGA GTTGTGGTCT GCCAGGATGA AAATGGACAA 
AGTGCCAGTT ACTGTGATGC AGCGTCCAAA CCCCCAGAGT CAAAGCACTG TGATTCAGGA 
CCTTGTCCAC AGTGGAACTA TGGAAGCTGG GGAGAATGTA CGCAAACGTG TGGAGGAGGA 
ATAAAATCAA GATTTGTAAT ATGCCAGTTT CCCGATGGCC AGCTGTCACA AGAGCAAAAC 
TGTGAAATGT TGAACAAGCC GCCTAGTGTG GTACAGTGTC ATATACATGC TTGCCCTGAT 
GACGTATCAT GGCATCGGGG ACCATGGAAA TCGTGCTCAG CTTCCTGTGG TAAAGGTTTA 
AAATACCGTG AGGTGCTTTG CATTGACCAA TTCCACAGGA AATTAGAAGA AAAATATTGC 
AGTCATCTAC AGAAACCTCG AACACATAAA GCTTGTAGAT CTGTCCGATG CCCTTCATGG 
AAAGCCAACA GATGGAAGGA GTGCTCTGTG ACCTGCGGCT CCGGAGTTCA GCGGAGGGAT 
GTGTACTGCA GACTCAGAGG AGTTGGTCGG GTGGCGGAAG AAAAGTGTGA TCCATCCACG 
CGGCCTTATT TTCAGAGAGA GTGTTGGCAT CGGGACTGCA TACAGTACCA CTGGGCGGCA 
GGAGAGTGGT TGGATTGCTC AGTATCGTGT AAGAAAAGAG AGACATATCG GCAAGTGAAG 
TGCATGGATG CACAAAACAT TCAAGTGAGT GAGAGTTTCT GTGACCTCGC GACCAGACCT 
CTCGCGGTGA AAAAGTGCAG AAACTCTCCC TGCAAGTATA TCGTGGTAAC AGGAGACTCA 
TCCCAGTGTG CAGGTAACTG TGGATTTAGT TATCGACAAA GGATTACATA TTGCGTTGGG 
GTCCAATCTA TGGAGAAACA TAAACTTCAT GAGCTGTGGC CTATAGACTA TCGAGTATGC 
CCTGTGCTGC CTTCCCCTCA GGTTTACAAA TGCAATTTTA GGACCTGTTT GCATATGGCC 
ACTTGGAAAG TTGGAAAATG GAGCAAGTGC TCTGTGACTT GTGGAGTTGG GATGATGGAG 
AGAAGAGTGG AATGCATGGC CGATAATGGT CTGTCCAGTG ATTTATGTTT AAAGCATTTA 
AAACCAGATG CTCAAAAGAA ATGTTATGTC AGTGACTGTA AAACATTTAT GAGCTGTAAA 
GAAATACAAG TGAAAAACAA CATTACCAAG GATGGTGACT ATTACCTTAA CATTAAAGGA 
AGAATAACAA AGATCTATTG TGCTGGCATG CACTTGGAGA ACCCTAAGGA ATATATATCA 
TTGGTCAAAG GTGAAGAAGA CAACTTTTCT GAAGTGTATG GTTTTAGACT ACGAAATCCA 
TATGAATGTC CTTTTAATGG AAGCAGGAGG CATGACTGTG AATGTAAAAA TGACTATCTG 
GCTGCTGGAC ACACTGTTTT TAGCAAAATA AGAATTGATC TTATTTCCAT GCAAATTAAA 
ACTACAGACC TTCTTTTTGC CCACACAATA TTTGGAAATG CAGTTCCATT TGCCACAGCT 
GGAGATTGCT ATAGTGCTGC CAGATGCCCA CAGGGACAGT TCAGCATTAA TTTGGCAGGA 
ACTGGGATGA AGATATCCAG TACAGCAAAG TGGCTGGCTC AGGGCAGTTA TGCCTCTGTG 
ATCATTCACA GATCACAGGA TGGAACCAAA GTCTATGGCA GATGTGGAGG GTTCTGTGGA 
AAGTGTATTC CTCACATGAC TACTGGTCTC CCAATTCAAG TAGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ADAMTS20 ( XM_012162373.2 ) cDNA ORF clone, Ovis aries musimon(Ovis orientalis musimon) -> XP_012017763.1 Ovis aries musimon ADAM metallopeptidase with thrombospondin type 1 motif 20 (ADAMTS20), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OOv33873
Clone ID Related Accession (Same CDS sequence)	XM_012162373.2 , XM_015089910.1
Accession Version	XM_012162373.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4665bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	A disintegrin and metalloproteinase with thrombospondin motifs 20 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942999.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012162373.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTTGG TCATGTGTCA GACAGGCTAC ACAGATCAAC AGACTAAGCA AAGAACTAGA 
AATTTGGAAC TCTTTTCTTT CATTTACTGT ATTGGATTAT ATGACAAGCA CTTCTGGCCT 
CTATCCCTTG TTATCCTATG CATGACCAGA TTTAATGTTC CACACGATGA TAGTTTTAAA 
TGTAAGGAAA CTGGAATTAA ACATCAGTAT CATGTCATGG CTCCAACTTT AAATTACCAC 
ACAAGTCCTT GGACCTGGTC AAAATGTAGT CAGAAATATA TCACTGAATT CCTAGACACC 
GGTCATGGGG AATGCCTCCT TGACAAACCA AATGGAAGAA TATACGATTT GTCTTCACAG 
CTTCCTGGAT TAATGTATGA TGTAAACAAG CAATGTGAGC TTATGTTTGG TCCTGGATCA 
CAAGTGTGTC CCTATTTAAA ACAATGCAGG CGTCTCTGGT GTACCAGCGC AGAAGGAGTC 
CACAAGGGCT GTCGCACTCA ACACATGCCT CTGGCAGACG GAACAAACTG TGGTCCTGGG 
ATGCATTGCC ACCATGGGCT ATGTGTAAAT AAAGAAATGG AGACACGTCC TGTAGATGGA 
GATTGGGGAC CATGGGGACC TTACAGTTCT TGTTCAAGAA CATGTGGAGG TGGAATCAAA 
AGCACAAGCA GGCTCTGTAA TCGCCCCGAG CCAAGAAACG GAGGCAAGTA CTGTGTGGGC 
CGCAGGATGA AATTTCGATC ATGTAACACT GATTCATGTC CAAAAGGCAA GCAAGACTTC 
CGTGAGAAGC AGTGCTCTGA TTTTGATGGT AAACATTTCA ACATCAATGG TCTCTCCCCT 
AACGTGAGGT GGCTTCCAAA GTACAGTGGG ATTGCTGTAA AAGATCGTTG TAAACTCTAT 
TGTCGGGTGG CTGGAACCAC TAGCTTCTAC CAACTGAAGG ACAGGGTTGC CGATGGTACT 
CCTTGTGGAA CGGAAACAAA TGACATCTGT GTTCAAGGCC TGTGTCGGCA AGCTGGCTGT 
GATCACGTGT TAAACTCCAA GGCCAGGAGA GACAAATGTG GCGTGTGTGG TGGGGATAAC 
TCCTCCTGCA GGACAATGGC AGGCGTCTTC AACAGTGCGC ATTATGGTTA TAATGTCGTT 
GTAAAGATTC CCCCAGGAGC AACAAACATT GATATTCTTC AGCACAGCTA TTCTGGAAAA 
CCAGAAGATG ACAATTACCT GGCATTATCG GACACTCAAG GGAATTTTCT TTTGAATGGG 
AATTTTGTTG TAAGTATGTC CAAACGGGAA ATCAACGTAC AAGGAGCTAT TTTTGAATAC 
AGTGGTTCAA ACAACTCAAT TGAAAGGATT AATAGTACTG ATCGACTGGA AGAAGAACTT 
GTTTTGCAGG TTTTGTGTGT GGGTAATCTA TACAACCCTG ATGTACGTTA TTCCTTCAAT 
ATCCCCGTGG AAGAGAAGAG TGATCTATTC ACATGGGACC CGTATGGACC ATGGCAAGAC 
TGTACAAAAA TGTGTCAAGG TCTTCATAGA AGAAAAATTA CTTGTGTACG TAAGAGTGAT 
CATATGGTCA TGTCTGATCA AAGCTGTGAC CACTTACCAC TTCCATTGCT GGTAACTGAA 
AGGTGCAATA CAGACTGTGA ACTCAGGTGG CACATCATTG GCAAAAGTGA ATGTTCAACC 
CTTTGTGGTC AAGGATACAA GTCATTGGAC GTCCATTGTA TGAAGTATTC CATTCATAAA 
GGACAGACTG TTCCAGTAGA TGACCACTAC TGCAGTGACC AACTTAAACC TCCAACCCGA 
GAACCCTGCC ATGGTGACTG TGTCTTAACA AGGTGGCATT ATTTAGAATG GTCTCAGTGT 
TCCAGGAGTT GCGGAGGAGG GGAGAGGTCT CGAGAATCTT ACTGTATAAA TAACTTTGGC 
CACCGTCTTG CTGACAGAGA ATGCCAGGAA CTTCCCCGAG TGACAACAGA GAATTGCAAT 
GAATTTTCCT GTCCCAGTTG GGCCACTAGT GAGTGGAGTG AGTGTCTGGT TACCTGTGGA 
AAAGGAACAA AGCAGCGGCA GGTGTGGTGT CACCTGAATG AAGATCACTT GAGTGATGGC 
TTCTGTGACC CAAGCACCAA GCCTGAGTCT CAGAGACCCT GTGAGCTTCA TGCATGTACT 
TCCTGGCAAG TAGGACCTTG GGGTTCTTGC ACAGCCACGT GTGGACAGGG GTACCAGATG 
CGTGCTGTGA AGTGTGTCAA CGAGCTATTT AGCACAGTGC TAGATGACAG ACTGTGCCAT 
GGAGCTAGTC GCCCAAGTGA AAGGCAGAAT TGTATTGTTA CACCTTGCCC AGTTATCCCT 
AAAATTGGGG CCACTTCCTT GCCAGCTGTT CCCATGAGAA AGATAACACA ATGGCGATAT 
GGTTCTTGGA CACCATGCTC CGTGTCTTGT GGAAGAGGAA ATCAAGCCCG CTATGTAAGT 
TGTCGTGATG CTCATGATGG AATAGCAGAT GAATCATTTT GTGCCCACTT ACCCCGACCG 
GCTGAAATAG CTGTCTGTTT TAGCCCTTGT GGAGAGTGGC AAACAGGGAA TTGGTCACCT 
TGTTCAGCTT CCTGTGGCCG TGGAAAAATG ACTCGACAAG TTTTATGCAT CAACTATCAT 
CAGCCAGTTA ATGAGAATTA CTGTGACCCT GAAGTCCGCC CTGTGATAGA ACAAGAATGT 
AATCTGGCAG CCTGCCCTCC CACGTACAGC CACTTTCCGA GTTCCTCTGA GCAGCCAAGC 
CATTTTCCAG GCAGGAATTT TCCATTAACT CACAAACCAG AAGATAATCA AAATCAGAGG 
GTCCATCCAT CAATCAGAGA AAACCAGTGG AGAACAGGAC CATGGGGATC ATGCTCTAGC 
AGTTGTGCTG GAGGTGTTCA GCGTAGAGTT GTGGTCTGCC AGGATGAAAA TGGACAAAGT 
GCCAGTTACT GTGATGCAGC GTCCAAACCC CCAGAGTCAA AGCACTGTGA TTCAGGACCT 
TGTCCACAGT GGAACTATGG AAGCTGGGGA GAATGTACGC AAACGTGTGG AGGAGGAATA 
AAATCAAGAT TTGTAATATG CCAGTTTCCC GATGGCCAGC TGTCACAAGA GCAAAACTGT 
GAAATGTTGA ACAAGCCGCC TAGTGTGGTA CAGTGTCATA TACATGCTTG CCCTGATGAC 
GTATCATGGC ATCGGGGACC ATGGAAATCG TGCTCAGCTT CCTGTGGTAA AGGTTTAAAA 
TACCGTGAGG TGCTTTGCAT TGACCAATTC CACAGGAAAT TAGAAGAAAA ATATTGCAGT 
CATCTACAGA AACCTCGAAC ACATAAAGCT TGTAGATCTG TCCGATGCCC TTCATGGAAA 
GCCAACAGAT GGAAGGAGTG CTCTGTGACC TGCGGCTCCG GAGTTCAGCG GAGGGATGTG 
TACTGCAGAC TCAGAGGAGT TGGTCGGGTG GCGGAAGAAA AGTGTGATCC ATCCACGCGG 
CCTTATTTTC AGAGAGAGTG TTGGCATCGG GACTGCATAC AGTACCACTG GGCGGCAGGA 
GAGTGGTTGG ATTGCTCAGT ATCGTGTAAG AAAAGAGAGA CATATCGGCA AGTGAAGTGC 
ATGGATGCAC AAAACATTCA AGTGAGTGAG AGTTTCTGTG ACCTCGCGAC CAGACCTCTC 
GCGGTGAAAA AGTGCAGAAA CTCTCCCTGC AAGTATATCG TGGTAACAGG AGACTCATCC 
CAGTGTGCAG GTAACTGTGG ATTTAGTTAT CGACAAAGGA TTACATATTG CGTTGGGGTC 
CAATCTATGG AGAAACATAA ACTTCATGAG CTGTGGCCTA TAGACTATCG AGTATGCCCT 
GTGCTGCCTT CCCCTCAGGT TTACAAATGC AATTTTAGGA CCTGTTTGCA TATGGCCACT 
TGGAAAGTTG GAAAATGGAG CAAGTGCTCT GTGACTTGTG GAGTTGGGAT GATGGAGAGA 
AGAGTGGAAT GCATGGCCGA TAATGGTCTG TCCAGTGATT TATGTTTAAA GCATTTAAAA 
CCAGATGCTC AAAAGAAATG TTATGTCAGT GACTGTAAAA CATTTATGAG CTGTAAAGAA 
ATACAAGTGA AAAACAACAT TACCAAGGAT GGTGACTATT ACCTTAACAT TAAAGGAAGA 
ATAACAAAGA TCTATTGTGC TGGCATGCAC TTGGAGAACC CTAAGGAATA TATATCATTG 
GTCAAAGGTG AAGAAGACAA CTTTTCTGAA GTGTATGGTT TTAGACTACG AAATCCATAT 
GAATGTCCTT TTAATGGAAG CAGGAGGCAT GACTGTGAAT GTAAAAATGA CTATCTGGCT 
GCTGGACACA CTGTTTTTAG CAAAATAAGA ATTGATCTTA TTTCCATGCA AATTAAAACT 
ACAGACCTTC TTTTTGCCCA CACAATATTT GGAAATGCAG TTCCATTTGC CACAGCTGGA 
GATTGCTATA GTGCTGCCAG ATGCCCACAG GGACAGTTCA GCATTAATTT GGCAGGAACT 
GGGATGAAGA TATCCAGTAC AGCAAAGTGG CTGGCTCAGG GCAGTTATGC CTCTGTGATC 
ATTCACAGAT CACAGGATGG AACCAAAGTC TATGGCAGAT GTGGAGGGTT CTGTGGAAAG 
TGTATTCCTC ACATGACTAC TGGTCTCCCA ATTCAAGTAG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012017763.1
CDS	739..5403
Translation	MSLVMCQTGYTDQQTKQRTRNLELFSFIYCIGLYDKHFWPLSLVILCMTRFNVPHDDSFKCKETGIKHQYHVMAPTLNYHTSPWTWSKCSQKYITEFLDTGHGECLLDKPNGRIYDLSSQLPGLMYDVNKQCELMFGPGSQVCPYLKQCRRLWCTSAEGVHKGCRTQHMPLADGTNCGPGMHCHHGLCVNKEMETRPVDGDWGPWGPYSSCSRTCGGGIKSTSRLCNRPEPRNGGKYCVGRRMKFRSCNTDSCPKGKQDFREKQCSDFDGKHFNINGLSPNVRWLPKYSGIAVKDRCKLYCRVAGTTSFYQLKDRVADGTPCGTETNDICVQGLCRQAGCDHVLNSKARRDKCGVCGGDNSSCRTMAGVFNSAHYGYNVVVKIPPGATNIDILQHSYSGKPEDDNYLALSDTQGNFLLNGNFVVSMSKREINVQGAIFEYSGSNNSIERINSTDRLEEELVLQVLCVGNLYNPDVRYSFNIPVEEKSDLFTWDPYGPWQDCTKMCQGLHRRKITCVRKSDHMVMSDQSCDHLPLPLLVTERCNTDCELRWHIIGKSECSTLCGQGYKSLDVHCMKYSIHKGQTVPVDDHYCSDQLKPPTREPCHGDCVLTRWHYLEWSQCSRSCGGGERSRESYCINNFGHRLADRECQELPRVTTENCNEFSCPSWATSEWSECLVTCGKGTKQRQVWCHLNEDHLSDGFCDPSTKPESQRPCELHACTSWQVGPWGSCTATCGQGYQMRAVKCVNELFSTVLDDRLCHGASRPSERQNCIVTPCPVIPKIGATSLPAVPMRKITQWRYGSWTPCSVSCGRGNQARYVSCRDAHDGIADESFCAHLPRPAEIAVCFSPCGEWQTGNWSPCSASCGRGKMTRQVLCINYHQPVNENYCDPEVRPVIEQECNLAACPPTYSHFPSSSEQPSHFPGRNFPLTHKPEDNQNQRVHPSIRENQWRTGPWGSCSSSCAGGVQRRVVVCQDENGQSASYCDAASKPPESKHCDSGPCPQWNYGSWGECTQTCGGGIKSRFVICQFPDGQLSQEQNCEMLNKPPSVVQCHIHACPDDVSWHRGPWKSCSASCGKGLKYREVLCIDQFHRKLEEKYCSHLQKPRTHKACRSVRCPSWKANRWKECSVTCGSGVQRRDVYCRLRGVGRVAEEKCDPSTRPYFQRECWHRDCIQYHWAAGEWLDCSVSCKKRETYRQVKCMDAQNIQVSESFCDLATRPLAVKKCRNSPCKYIVVTGDSSQCAGNCGFSYRQRITYCVGVQSMEKHKLHELWPIDYRVCPVLPSPQVYKCNFRTCLHMATWKVGKWSKCSVTCGVGMMERRVECMADNGLSSDLCLKHLKPDAQKKCYVSDCKTFMSCKEIQVKNNITKDGDYYLNIKGRITKIYCAGMHLENPKEYISLVKGEEDNFSEVYGFRLRNPYECPFNGSRRHDCECKNDYLAAGHTVFSKIRIDLISMQIKTTDLLFAHTIFGNAVPFATAGDCYSAARCPQGQFSINLAGTGMKISSTAKWLAQGSYASVIIHRSQDGTKVYGRCGGFCGKCIPHMTTGLPIQVV

Target ORF information:

RefSeq Version	XM_012162373.2
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon ADAM metallopeptidase with thrombospondin type 1 motif 20 (ADAMTS20), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012162373.2

ORF Insert Sequence:

ATGAGCTTGG TCATGTGTCA GACAGGCTAC ACAGATCAAC AGACTAAGCA AAGAACTAGA 
AATTTGGAAC TCTTTTCTTT CATTTACTGT ATTGGATTAT ATGACAAGCA CTTCTGGCCT 
CTATCCCTTG TTATCCTATG CATGACCAGA TTTAATGTTC CACACGATGA TAGTTTTAAA 
TGTAAGGAAA CTGGAATTAA ACATCAGTAT CATGTCATGG CTCCAACTTT AAATTACCAC 
ACAAGTCCTT GGACCTGGTC AAAATGTAGT CAGAAATATA TCACTGAATT CCTAGACACC 
GGTCATGGGG AATGCCTCCT TGACAAACCA AATGGAAGAA TATACGATTT GTCTTCACAG 
CTTCCTGGAT TAATGTATGA TGTAAACAAG CAATGTGAGC TTATGTTTGG TCCTGGATCA 
CAAGTGTGTC CCTATTTAAA ACAATGCAGG CGTCTCTGGT GTACCAGCGC AGAAGGAGTC 
CACAAGGGCT GTCGCACTCA ACACATGCCT CTGGCAGACG GAACAAACTG TGGTCCTGGG 
ATGCATTGCC ACCATGGGCT ATGTGTAAAT AAAGAAATGG AGACACGTCC TGTAGATGGA 
GATTGGGGAC CATGGGGACC TTACAGTTCT TGTTCAAGAA CATGTGGAGG TGGAATCAAA 
AGCACAAGCA GGCTCTGTAA TCGCCCCGAG CCAAGAAACG GAGGCAAGTA CTGTGTGGGC 
CGCAGGATGA AATTTCGATC ATGTAACACT GATTCATGTC CAAAAGGCAA GCAAGACTTC 
CGTGAGAAGC AGTGCTCTGA TTTTGATGGT AAACATTTCA ACATCAATGG TCTCTCCCCT 
AACGTGAGGT GGCTTCCAAA GTACAGTGGG ATTGCTGTAA AAGATCGTTG TAAACTCTAT 
TGTCGGGTGG CTGGAACCAC TAGCTTCTAC CAACTGAAGG ACAGGGTTGC CGATGGTACT 
CCTTGTGGAA CGGAAACAAA TGACATCTGT GTTCAAGGCC TGTGTCGGCA AGCTGGCTGT 
GATCACGTGT TAAACTCCAA GGCCAGGAGA GACAAATGTG GCGTGTGTGG TGGGGATAAC 
TCCTCCTGCA GGACAATGGC AGGCGTCTTC AACAGTGCGC ATTATGGTTA TAATGTCGTT 
GTAAAGATTC CCCCAGGAGC AACAAACATT GATATTCTTC AGCACAGCTA TTCTGGAAAA 
CCAGAAGATG ACAATTACCT GGCATTATCG GACACTCAAG GGAATTTTCT TTTGAATGGG 
AATTTTGTTG TAAGTATGTC CAAACGGGAA ATCAACGTAC AAGGAGCTAT TTTTGAATAC 
AGTGGTTCAA ACAACTCAAT TGAAAGGATT AATAGTACTG ATCGACTGGA AGAAGAACTT 
GTTTTGCAGG TTTTGTGTGT GGGTAATCTA TACAACCCTG ATGTACGTTA TTCCTTCAAT 
ATCCCCGTGG AAGAGAAGAG TGATCTATTC ACATGGGACC CGTATGGACC ATGGCAAGAC 
TGTACAAAAA TGTGTCAAGG TCTTCATAGA AGAAAAATTA CTTGTGTACG TAAGAGTGAT 
CATATGGTCA TGTCTGATCA AAGCTGTGAC CACTTACCAC TTCCATTGCT GGTAACTGAA 
AGGTGCAATA CAGACTGTGA ACTCAGGTGG CACATCATTG GCAAAAGTGA ATGTTCAACC 
CTTTGTGGTC AAGGATACAA GTCATTGGAC GTCCATTGTA TGAAGTATTC CATTCATAAA 
GGACAGACTG TTCCAGTAGA TGACCACTAC TGCAGTGACC AACTTAAACC TCCAACCCGA 
GAACCCTGCC ATGGTGACTG TGTCTTAACA AGGTGGCATT ATTTAGAATG GTCTCAGTGT 
TCCAGGAGTT GCGGAGGAGG GGAGAGGTCT CGAGAATCTT ACTGTATAAA TAACTTTGGC 
CACCGTCTTG CTGACAGAGA ATGCCAGGAA CTTCCCCGAG TGACAACAGA GAATTGCAAT 
GAATTTTCCT GTCCCAGTTG GGCCACTAGT GAGTGGAGTG AGTGTCTGGT TACCTGTGGA 
AAAGGAACAA AGCAGCGGCA GGTGTGGTGT CACCTGAATG AAGATCACTT GAGTGATGGC 
TTCTGTGACC CAAGCACCAA GCCTGAGTCT CAGAGACCCT GTGAGCTTCA TGCATGTACT 
TCCTGGCAAG TAGGACCTTG GGGTTCTTGC ACAGCCACGT GTGGACAGGG GTACCAGATG 
CGTGCTGTGA AGTGTGTCAA CGAGCTATTT AGCACAGTGC TAGATGACAG ACTGTGCCAT 
GGAGCTAGTC GCCCAAGTGA AAGGCAGAAT TGTATTGTTA CACCTTGCCC AGTTATCCCT 
AAAATTGGGG CCACTTCCTT GCCAGCTGTT CCCATGAGAA AGATAACACA ATGGCGATAT 
GGTTCTTGGA CACCATGCTC CGTGTCTTGT GGAAGAGGAA ATCAAGCCCG CTATGTAAGT 
TGTCGTGATG CTCATGATGG AATAGCAGAT GAATCATTTT GTGCCCACTT ACCCCGACCG 
GCTGAAATAG CTGTCTGTTT TAGCCCTTGT GGAGAGTGGC AAACAGGGAA TTGGTCACCT 
TGTTCAGCTT CCTGTGGCCG TGGAAAAATG ACTCGACAAG TTTTATGCAT CAACTATCAT 
CAGCCAGTTA ATGAGAATTA CTGTGACCCT GAAGTCCGCC CTGTGATAGA ACAAGAATGT 
AATCTGGCAG CCTGCCCTCC CACGTACAGC CACTTTCCGA GTTCCTCTGA GCAGCCAAGC 
CATTTTCCAG GCAGGAATTT TCCATTAACT CACAAACCAG AAGATAATCA AAATCAGAGG 
GTCCATCCAT CAATCAGAGA AAACCAGTGG AGAACAGGAC CATGGGGATC ATGCTCTAGC 
AGTTGTGCTG GAGGTGTTCA GCGTAGAGTT GTGGTCTGCC AGGATGAAAA TGGACAAAGT 
GCCAGTTACT GTGATGCAGC GTCCAAACCC CCAGAGTCAA AGCACTGTGA TTCAGGACCT 
TGTCCACAGT GGAACTATGG AAGCTGGGGA GAATGTACGC AAACGTGTGG AGGAGGAATA 
AAATCAAGAT TTGTAATATG CCAGTTTCCC GATGGCCAGC TGTCACAAGA GCAAAACTGT 
GAAATGTTGA ACAAGCCGCC TAGTGTGGTA CAGTGTCATA TACATGCTTG CCCTGATGAC 
GTATCATGGC ATCGGGGACC ATGGAAATCG TGCTCAGCTT CCTGTGGTAA AGGTTTAAAA 
TACCGTGAGG TGCTTTGCAT TGACCAATTC CACAGGAAAT TAGAAGAAAA ATATTGCAGT 
CATCTACAGA AACCTCGAAC ACATAAAGCT TGTAGATCTG TCCGATGCCC TTCATGGAAA 
GCCAACAGAT GGAAGGAGTG CTCTGTGACC TGCGGCTCCG GAGTTCAGCG GAGGGATGTG 
TACTGCAGAC TCAGAGGAGT TGGTCGGGTG GCGGAAGAAA AGTGTGATCC ATCCACGCGG 
CCTTATTTTC AGAGAGAGTG TTGGCATCGG GACTGCATAC AGTACCACTG GGCGGCAGGA 
GAGTGGTTGG ATTGCTCAGT ATCGTGTAAG AAAAGAGAGA CATATCGGCA AGTGAAGTGC 
ATGGATGCAC AAAACATTCA AGTGAGTGAG AGTTTCTGTG ACCTCGCGAC CAGACCTCTC 
GCGGTGAAAA AGTGCAGAAA CTCTCCCTGC AAGTATATCG TGGTAACAGG AGACTCATCC 
CAGTGTGCAG GTAACTGTGG ATTTAGTTAT CGACAAAGGA TTACATATTG CGTTGGGGTC 
CAATCTATGG AGAAACATAA ACTTCATGAG CTGTGGCCTA TAGACTATCG AGTATGCCCT 
GTGCTGCCTT CCCCTCAGGT TTACAAATGC AATTTTAGGA CCTGTTTGCA TATGGCCACT 
TGGAAAGTTG GAAAATGGAG CAAGTGCTCT GTGACTTGTG GAGTTGGGAT GATGGAGAGA 
AGAGTGGAAT GCATGGCCGA TAATGGTCTG TCCAGTGATT TATGTTTAAA GCATTTAAAA 
CCAGATGCTC AAAAGAAATG TTATGTCAGT GACTGTAAAA CATTTATGAG CTGTAAAGAA 
ATACAAGTGA AAAACAACAT TACCAAGGAT GGTGACTATT ACCTTAACAT TAAAGGAAGA 
ATAACAAAGA TCTATTGTGC TGGCATGCAC TTGGAGAACC CTAAGGAATA TATATCATTG 
GTCAAAGGTG AAGAAGACAA CTTTTCTGAA GTGTATGGTT TTAGACTACG AAATCCATAT 
GAATGTCCTT TTAATGGAAG CAGGAGGCAT GACTGTGAAT GTAAAAATGA CTATCTGGCT 
GCTGGACACA CTGTTTTTAG CAAAATAAGA ATTGATCTTA TTTCCATGCA AATTAAAACT 
ACAGACCTTC TTTTTGCCCA CACAATATTT GGAAATGCAG TTCCATTTGC CACAGCTGGA 
GATTGCTATA GTGCTGCCAG ATGCCCACAG GGACAGTTCA GCATTAATTT GGCAGGAACT 
GGGATGAAGA TATCCAGTAC AGCAAAGTGG CTGGCTCAGG GCAGTTATGC CTCTGTGATC 
ATTCACAGAT CACAGGATGG AACCAAAGTC TATGGCAGAT GTGGAGGGTT CTGTGGAAAG 
TGTATTCCTC ACATGACTAC TGGTCTCCCA ATTCAAGTAG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ADAMTS20 ( XM_015089910.1 ) cDNA ORF clone, Ovis aries musimon(Ovis orientalis musimon) -> XP_014945396.1 Ovis aries musimon ADAM metallopeptidase with thrombospondin type 1 motif 20 (ADAMTS20), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OOv33873
Clone ID Related Accession (Same CDS sequence)	XM_012162373.2 , XM_015089910.1
Accession Version	XM_015089910.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4665bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	A disintegrin and metalloproteinase with thrombospondin motifs 20 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942999.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTTGG TCATGTGTCA GACAGGCTAC ACAGATCAAC AGACTAAGCA AAGAACTAGA 
AATTTGGAAC TCTTTTCTTT CATTTACTGT ATTGGATTAT ATGACAAGCA CTTCTGGCCT 
CTATCCCTTG TTATCCTATG CATGACCAGA TTTAATGTTC CACACGATGA TAGTTTTAAA 
TGTAAGGAAA CTGGAATTAA ACATCAGTAT CATGTCATGG CTCCAACTTT AAATTACCAC 
ACAAGTCCTT GGACCTGGTC AAAATGTAGT CAGAAATATA TCACTGAATT CCTAGACACC 
GGTCATGGGG AATGCCTCCT TGACAAACCA AATGGAAGAA TATACGATTT GTCTTCACAG 
CTTCCTGGAT TAATGTATGA TGTAAACAAG CAATGTGAGC TTATGTTTGG TCCTGGATCA 
CAAGTGTGTC CCTATTTAAA ACAATGCAGG CGTCTCTGGT GTACCAGCGC AGAAGGAGTC 
CACAAGGGCT GTCGCACTCA ACACATGCCT CTGGCAGACG GAACAAACTG TGGTCCTGGG 
ATGCATTGCC ACCATGGGCT ATGTGTAAAT AAAGAAATGG AGACACGTCC TGTAGATGGA 
GATTGGGGAC CATGGGGACC TTACAGTTCT TGTTCAAGAA CATGTGGAGG TGGAATCAAA 
AGCACAAGCA GGCTCTGTAA TCGCCCCGAG CCAAGAAACG GAGGCAAGTA CTGTGTGGGC 
CGCAGGATGA AATTTCGATC ATGTAACACT GATTCATGTC CAAAAGGCAA GCAAGACTTC 
CGTGAGAAGC AGTGCTCTGA TTTTGATGGT AAACATTTCA ACATCAATGG TCTCTCCCCT 
AACGTGAGGT GGCTTCCAAA GTACAGTGGG ATTGCTGTAA AAGATCGTTG TAAACTCTAT 
TGTCGGGTGG CTGGAACCAC TAGCTTCTAC CAACTGAAGG ACAGGGTTGC CGATGGTACT 
CCTTGTGGAA CGGAAACAAA TGACATCTGT GTTCAAGGCC TGTGTCGGCA AGCTGGCTGT 
GATCACGTGT TAAACTCCAA GGCCAGGAGA GACAAATGTG GCGTGTGTGG TGGGGATAAC 
TCCTCCTGCA GGACAATGGC AGGCGTCTTC AACAGTGCGC ATTATGGTTA TAATGTCGTT 
GTAAAGATTC CCCCAGGAGC AACAAACATT GATATTCTTC AGCACAGCTA TTCTGGAAAA 
CCAGAAGATG ACAATTACCT GGCATTATCG GACACTCAAG GGAATTTTCT TTTGAATGGG 
AATTTTGTTG TAAGTATGTC CAAACGGGAA ATCAACGTAC AAGGAGCTAT TTTTGAATAC 
AGTGGTTCAA ACAACTCAAT TGAAAGGATT AATAGTACTG ATCGACTGGA AGAAGAACTT 
GTTTTGCAGG TTTTGTGTGT GGGTAATCTA TACAACCCTG ATGTACGTTA TTCCTTCAAT 
ATCCCCGTGG AAGAGAAGAG TGATCTATTC ACATGGGACC CGTATGGACC ATGGCAAGAC 
TGTACAAAAA TGTGTCAAGG TCTTCATAGA AGAAAAATTA CTTGTGTACG TAAGAGTGAT 
CATATGGTCA TGTCTGATCA AAGCTGTGAC CACTTACCAC TTCCATTGCT GGTAACTGAA 
AGGTGCAATA CAGACTGTGA ACTCAGGTGG CACATCATTG GCAAAAGTGA ATGTTCAACC 
CTTTGTGGTC AAGGATACAA GTCATTGGAC GTCCATTGTA TGAAGTATTC CATTCATAAA 
GGACAGACTG TTCCAGTAGA TGACCACTAC TGCAGTGACC AACTTAAACC TCCAACCCGA 
GAACCCTGCC ATGGTGACTG TGTCTTAACA AGGTGGCATT ATTTAGAATG GTCTCAGTGT 
TCCAGGAGTT GCGGAGGAGG GGAGAGGTCT CGAGAATCTT ACTGTATAAA TAACTTTGGC 
CACCGTCTTG CTGACAGAGA ATGCCAGGAA CTTCCCCGAG TGACAACAGA GAATTGCAAT 
GAATTTTCCT GTCCCAGTTG GGCCACTAGT GAGTGGAGTG AGTGTCTGGT TACCTGTGGA 
AAAGGAACAA AGCAGCGGCA GGTGTGGTGT CACCTGAATG AAGATCACTT GAGTGATGGC 
TTCTGTGACC CAAGCACCAA GCCTGAGTCT CAGAGACCCT GTGAGCTTCA TGCATGTACT 
TCCTGGCAAG TAGGACCTTG GGGTTCTTGC ACAGCCACGT GTGGACAGGG GTACCAGATG 
CGTGCTGTGA AGTGTGTCAA CGAGCTATTT AGCACAGTGC TAGATGACAG ACTGTGCCAT 
GGAGCTAGTC GCCCAAGTGA AAGGCAGAAT TGTATTGTTA CACCTTGCCC AGTTATCCCT 
AAAATTGGGG CCACTTCCTT GCCAGCTGTT CCCATGAGAA AGATAACACA ATGGCGATAT 
GGTTCTTGGA CACCATGCTC CGTGTCTTGT GGAAGAGGAA ATCAAGCCCG CTATGTAAGT 
TGTCGTGATG CTCATGATGG AATAGCAGAT GAATCATTTT GTGCCCACTT ACCCCGACCG 
GCTGAAATAG CTGTCTGTTT TAGCCCTTGT GGAGAGTGGC AAACAGGGAA TTGGTCACCT 
TGTTCAGCTT CCTGTGGCCG TGGAAAAATG ACTCGACAAG TTTTATGCAT CAACTATCAT 
CAGCCAGTTA ATGAGAATTA CTGTGACCCT GAAGTCCGCC CTGTGATAGA ACAAGAATGT 
AATCTGGCAG CCTGCCCTCC CACGTACAGC CACTTTCCGA GTTCCTCTGA GCAGCCAAGC 
CATTTTCCAG GCAGGAATTT TCCATTAACT CACAAACCAG AAGATAATCA AAATCAGAGG 
GTCCATCCAT CAATCAGAGA AAACCAGTGG AGAACAGGAC CATGGGGATC ATGCTCTAGC 
AGTTGTGCTG GAGGTGTTCA GCGTAGAGTT GTGGTCTGCC AGGATGAAAA TGGACAAAGT 
GCCAGTTACT GTGATGCAGC GTCCAAACCC CCAGAGTCAA AGCACTGTGA TTCAGGACCT 
TGTCCACAGT GGAACTATGG AAGCTGGGGA GAATGTACGC AAACGTGTGG AGGAGGAATA 
AAATCAAGAT TTGTAATATG CCAGTTTCCC GATGGCCAGC TGTCACAAGA GCAAAACTGT 
GAAATGTTGA ACAAGCCGCC TAGTGTGGTA CAGTGTCATA TACATGCTTG CCCTGATGAC 
GTATCATGGC ATCGGGGACC ATGGAAATCG TGCTCAGCTT CCTGTGGTAA AGGTTTAAAA 
TACCGTGAGG TGCTTTGCAT TGACCAATTC CACAGGAAAT TAGAAGAAAA ATATTGCAGT 
CATCTACAGA AACCTCGAAC ACATAAAGCT TGTAGATCTG TCCGATGCCC TTCATGGAAA 
GCCAACAGAT GGAAGGAGTG CTCTGTGACC TGCGGCTCCG GAGTTCAGCG GAGGGATGTG 
TACTGCAGAC TCAGAGGAGT TGGTCGGGTG GCGGAAGAAA AGTGTGATCC ATCCACGCGG 
CCTTATTTTC AGAGAGAGTG TTGGCATCGG GACTGCATAC AGTACCACTG GGCGGCAGGA 
GAGTGGTTGG ATTGCTCAGT ATCGTGTAAG AAAAGAGAGA CATATCGGCA AGTGAAGTGC 
ATGGATGCAC AAAACATTCA AGTGAGTGAG AGTTTCTGTG ACCTCGCGAC CAGACCTCTC 
GCGGTGAAAA AGTGCAGAAA CTCTCCCTGC AAGTATATCG TGGTAACAGG AGACTCATCC 
CAGTGTGCAG GTAACTGTGG ATTTAGTTAT CGACAAAGGA TTACATATTG CGTTGGGGTC 
CAATCTATGG AGAAACATAA ACTTCATGAG CTGTGGCCTA TAGACTATCG AGTATGCCCT 
GTGCTGCCTT CCCCTCAGGT TTACAAATGC AATTTTAGGA CCTGTTTGCA TATGGCCACT 
TGGAAAGTTG GAAAATGGAG CAAGTGCTCT GTGACTTGTG GAGTTGGGAT GATGGAGAGA 
AGAGTGGAAT GCATGGCCGA TAATGGTCTG TCCAGTGATT TATGTTTAAA GCATTTAAAA 
CCAGATGCTC AAAAGAAATG TTATGTCAGT GACTGTAAAA CATTTATGAG CTGTAAAGAA 
ATACAAGTGA AAAACAACAT TACCAAGGAT GGTGACTATT ACCTTAACAT TAAAGGAAGA 
ATAACAAAGA TCTATTGTGC TGGCATGCAC TTGGAGAACC CTAAGGAATA TATATCATTG 
GTCAAAGGTG AAGAAGACAA CTTTTCTGAA GTGTATGGTT TTAGACTACG AAATCCATAT 
GAATGTCCTT TTAATGGAAG CAGGAGGCAT GACTGTGAAT GTAAAAATGA CTATCTGGCT 
GCTGGACACA CTGTTTTTAG CAAAATAAGA ATTGATCTTA TTTCCATGCA AATTAAAACT 
ACAGACCTTC TTTTTGCCCA CACAATATTT GGAAATGCAG TTCCATTTGC CACAGCTGGA 
GATTGCTATA GTGCTGCCAG ATGCCCACAG GGACAGTTCA GCATTAATTT GGCAGGAACT 
GGGATGAAGA TATCCAGTAC AGCAAAGTGG CTGGCTCAGG GCAGTTATGC CTCTGTGATC 
ATTCACAGAT CACAGGATGG AACCAAAGTC TATGGCAGAT GTGGAGGGTT CTGTGGAAAG 
TGTATTCCTC ACATGACTAC TGGTCTCCCA ATTCAAGTAG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014945396.1
CDS	3547..8211
Translation	MSLVMCQTGYTDQQTKQRTRNLELFSFIYCIGLYDKHFWPLSLVILCMTRFNVPHDDSFKCKETGIKHQYHVMAPTLNYHTSPWTWSKCSQKYITEFLDTGHGECLLDKPNGRIYDLSSQLPGLMYDVNKQCELMFGPGSQVCPYLKQCRRLWCTSAEGVHKGCRTQHMPLADGTNCGPGMHCHHGLCVNKEMETRPVDGDWGPWGPYSSCSRTCGGGIKSTSRLCNRPEPRNGGKYCVGRRMKFRSCNTDSCPKGKQDFREKQCSDFDGKHFNINGLSPNVRWLPKYSGIAVKDRCKLYCRVAGTTSFYQLKDRVADGTPCGTETNDICVQGLCRQAGCDHVLNSKARRDKCGVCGGDNSSCRTMAGVFNSAHYGYNVVVKIPPGATNIDILQHSYSGKPEDDNYLALSDTQGNFLLNGNFVVSMSKREINVQGAIFEYSGSNNSIERINSTDRLEEELVLQVLCVGNLYNPDVRYSFNIPVEEKSDLFTWDPYGPWQDCTKMCQGLHRRKITCVRKSDHMVMSDQSCDHLPLPLLVTERCNTDCELRWHIIGKSECSTLCGQGYKSLDVHCMKYSIHKGQTVPVDDHYCSDQLKPPTREPCHGDCVLTRWHYLEWSQCSRSCGGGERSRESYCINNFGHRLADRECQELPRVTTENCNEFSCPSWATSEWSECLVTCGKGTKQRQVWCHLNEDHLSDGFCDPSTKPESQRPCELHACTSWQVGPWGSCTATCGQGYQMRAVKCVNELFSTVLDDRLCHGASRPSERQNCIVTPCPVIPKIGATSLPAVPMRKITQWRYGSWTPCSVSCGRGNQARYVSCRDAHDGIADESFCAHLPRPAEIAVCFSPCGEWQTGNWSPCSASCGRGKMTRQVLCINYHQPVNENYCDPEVRPVIEQECNLAACPPTYSHFPSSSEQPSHFPGRNFPLTHKPEDNQNQRVHPSIRENQWRTGPWGSCSSSCAGGVQRRVVVCQDENGQSASYCDAASKPPESKHCDSGPCPQWNYGSWGECTQTCGGGIKSRFVICQFPDGQLSQEQNCEMLNKPPSVVQCHIHACPDDVSWHRGPWKSCSASCGKGLKYREVLCIDQFHRKLEEKYCSHLQKPRTHKACRSVRCPSWKANRWKECSVTCGSGVQRRDVYCRLRGVGRVAEEKCDPSTRPYFQRECWHRDCIQYHWAAGEWLDCSVSCKKRETYRQVKCMDAQNIQVSESFCDLATRPLAVKKCRNSPCKYIVVTGDSSQCAGNCGFSYRQRITYCVGVQSMEKHKLHELWPIDYRVCPVLPSPQVYKCNFRTCLHMATWKVGKWSKCSVTCGVGMMERRVECMADNGLSSDLCLKHLKPDAQKKCYVSDCKTFMSCKEIQVKNNITKDGDYYLNIKGRITKIYCAGMHLENPKEYISLVKGEEDNFSEVYGFRLRNPYECPFNGSRRHDCECKNDYLAAGHTVFSKIRIDLISMQIKTTDLLFAHTIFGNAVPFATAGDCYSAARCPQGQFSINLAGTGMKISSTAKWLAQGSYASVIIHRSQDGTKVYGRCGGFCGKCIPHMTTGLPIQVV

Target ORF information:

RefSeq Version	XM_015089910.1
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon ADAM metallopeptidase with thrombospondin type 1 motif 20 (ADAMTS20), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015089910.1

ORF Insert Sequence:

ATGAGCTTGG TCATGTGTCA GACAGGCTAC ACAGATCAAC AGACTAAGCA AAGAACTAGA 
AATTTGGAAC TCTTTTCTTT CATTTACTGT ATTGGATTAT ATGACAAGCA CTTCTGGCCT 
CTATCCCTTG TTATCCTATG CATGACCAGA TTTAATGTTC CACACGATGA TAGTTTTAAA 
TGTAAGGAAA CTGGAATTAA ACATCAGTAT CATGTCATGG CTCCAACTTT AAATTACCAC 
ACAAGTCCTT GGACCTGGTC AAAATGTAGT CAGAAATATA TCACTGAATT CCTAGACACC 
GGTCATGGGG AATGCCTCCT TGACAAACCA AATGGAAGAA TATACGATTT GTCTTCACAG 
CTTCCTGGAT TAATGTATGA TGTAAACAAG CAATGTGAGC TTATGTTTGG TCCTGGATCA 
CAAGTGTGTC CCTATTTAAA ACAATGCAGG CGTCTCTGGT GTACCAGCGC AGAAGGAGTC 
CACAAGGGCT GTCGCACTCA ACACATGCCT CTGGCAGACG GAACAAACTG TGGTCCTGGG 
ATGCATTGCC ACCATGGGCT ATGTGTAAAT AAAGAAATGG AGACACGTCC TGTAGATGGA 
GATTGGGGAC CATGGGGACC TTACAGTTCT TGTTCAAGAA CATGTGGAGG TGGAATCAAA 
AGCACAAGCA GGCTCTGTAA TCGCCCCGAG CCAAGAAACG GAGGCAAGTA CTGTGTGGGC 
CGCAGGATGA AATTTCGATC ATGTAACACT GATTCATGTC CAAAAGGCAA GCAAGACTTC 
CGTGAGAAGC AGTGCTCTGA TTTTGATGGT AAACATTTCA ACATCAATGG TCTCTCCCCT 
AACGTGAGGT GGCTTCCAAA GTACAGTGGG ATTGCTGTAA AAGATCGTTG TAAACTCTAT 
TGTCGGGTGG CTGGAACCAC TAGCTTCTAC CAACTGAAGG ACAGGGTTGC CGATGGTACT 
CCTTGTGGAA CGGAAACAAA TGACATCTGT GTTCAAGGCC TGTGTCGGCA AGCTGGCTGT 
GATCACGTGT TAAACTCCAA GGCCAGGAGA GACAAATGTG GCGTGTGTGG TGGGGATAAC 
TCCTCCTGCA GGACAATGGC AGGCGTCTTC AACAGTGCGC ATTATGGTTA TAATGTCGTT 
GTAAAGATTC CCCCAGGAGC AACAAACATT GATATTCTTC AGCACAGCTA TTCTGGAAAA 
CCAGAAGATG ACAATTACCT GGCATTATCG GACACTCAAG GGAATTTTCT TTTGAATGGG 
AATTTTGTTG TAAGTATGTC CAAACGGGAA ATCAACGTAC AAGGAGCTAT TTTTGAATAC 
AGTGGTTCAA ACAACTCAAT TGAAAGGATT AATAGTACTG ATCGACTGGA AGAAGAACTT 
GTTTTGCAGG TTTTGTGTGT GGGTAATCTA TACAACCCTG ATGTACGTTA TTCCTTCAAT 
ATCCCCGTGG AAGAGAAGAG TGATCTATTC ACATGGGACC CGTATGGACC ATGGCAAGAC 
TGTACAAAAA TGTGTCAAGG TCTTCATAGA AGAAAAATTA CTTGTGTACG TAAGAGTGAT 
CATATGGTCA TGTCTGATCA AAGCTGTGAC CACTTACCAC TTCCATTGCT GGTAACTGAA 
AGGTGCAATA CAGACTGTGA ACTCAGGTGG CACATCATTG GCAAAAGTGA ATGTTCAACC 
CTTTGTGGTC AAGGATACAA GTCATTGGAC GTCCATTGTA TGAAGTATTC CATTCATAAA 
GGACAGACTG TTCCAGTAGA TGACCACTAC TGCAGTGACC AACTTAAACC TCCAACCCGA 
GAACCCTGCC ATGGTGACTG TGTCTTAACA AGGTGGCATT ATTTAGAATG GTCTCAGTGT 
TCCAGGAGTT GCGGAGGAGG GGAGAGGTCT CGAGAATCTT ACTGTATAAA TAACTTTGGC 
CACCGTCTTG CTGACAGAGA ATGCCAGGAA CTTCCCCGAG TGACAACAGA GAATTGCAAT 
GAATTTTCCT GTCCCAGTTG GGCCACTAGT GAGTGGAGTG AGTGTCTGGT TACCTGTGGA 
AAAGGAACAA AGCAGCGGCA GGTGTGGTGT CACCTGAATG AAGATCACTT GAGTGATGGC 
TTCTGTGACC CAAGCACCAA GCCTGAGTCT CAGAGACCCT GTGAGCTTCA TGCATGTACT 
TCCTGGCAAG TAGGACCTTG GGGTTCTTGC ACAGCCACGT GTGGACAGGG GTACCAGATG 
CGTGCTGTGA AGTGTGTCAA CGAGCTATTT AGCACAGTGC TAGATGACAG ACTGTGCCAT 
GGAGCTAGTC GCCCAAGTGA AAGGCAGAAT TGTATTGTTA CACCTTGCCC AGTTATCCCT 
AAAATTGGGG CCACTTCCTT GCCAGCTGTT CCCATGAGAA AGATAACACA ATGGCGATAT 
GGTTCTTGGA CACCATGCTC CGTGTCTTGT GGAAGAGGAA ATCAAGCCCG CTATGTAAGT 
TGTCGTGATG CTCATGATGG AATAGCAGAT GAATCATTTT GTGCCCACTT ACCCCGACCG 
GCTGAAATAG CTGTCTGTTT TAGCCCTTGT GGAGAGTGGC AAACAGGGAA TTGGTCACCT 
TGTTCAGCTT CCTGTGGCCG TGGAAAAATG ACTCGACAAG TTTTATGCAT CAACTATCAT 
CAGCCAGTTA ATGAGAATTA CTGTGACCCT GAAGTCCGCC CTGTGATAGA ACAAGAATGT 
AATCTGGCAG CCTGCCCTCC CACGTACAGC CACTTTCCGA GTTCCTCTGA GCAGCCAAGC 
CATTTTCCAG GCAGGAATTT TCCATTAACT CACAAACCAG AAGATAATCA AAATCAGAGG 
GTCCATCCAT CAATCAGAGA AAACCAGTGG AGAACAGGAC CATGGGGATC ATGCTCTAGC 
AGTTGTGCTG GAGGTGTTCA GCGTAGAGTT GTGGTCTGCC AGGATGAAAA TGGACAAAGT 
GCCAGTTACT GTGATGCAGC GTCCAAACCC CCAGAGTCAA AGCACTGTGA TTCAGGACCT 
TGTCCACAGT GGAACTATGG AAGCTGGGGA GAATGTACGC AAACGTGTGG AGGAGGAATA 
AAATCAAGAT TTGTAATATG CCAGTTTCCC GATGGCCAGC TGTCACAAGA GCAAAACTGT 
GAAATGTTGA ACAAGCCGCC TAGTGTGGTA CAGTGTCATA TACATGCTTG CCCTGATGAC 
GTATCATGGC ATCGGGGACC ATGGAAATCG TGCTCAGCTT CCTGTGGTAA AGGTTTAAAA 
TACCGTGAGG TGCTTTGCAT TGACCAATTC CACAGGAAAT TAGAAGAAAA ATATTGCAGT 
CATCTACAGA AACCTCGAAC ACATAAAGCT TGTAGATCTG TCCGATGCCC TTCATGGAAA 
GCCAACAGAT GGAAGGAGTG CTCTGTGACC TGCGGCTCCG GAGTTCAGCG GAGGGATGTG 
TACTGCAGAC TCAGAGGAGT TGGTCGGGTG GCGGAAGAAA AGTGTGATCC ATCCACGCGG 
CCTTATTTTC AGAGAGAGTG TTGGCATCGG GACTGCATAC AGTACCACTG GGCGGCAGGA 
GAGTGGTTGG ATTGCTCAGT ATCGTGTAAG AAAAGAGAGA CATATCGGCA AGTGAAGTGC 
ATGGATGCAC AAAACATTCA AGTGAGTGAG AGTTTCTGTG ACCTCGCGAC CAGACCTCTC 
GCGGTGAAAA AGTGCAGAAA CTCTCCCTGC AAGTATATCG TGGTAACAGG AGACTCATCC 
CAGTGTGCAG GTAACTGTGG ATTTAGTTAT CGACAAAGGA TTACATATTG CGTTGGGGTC 
CAATCTATGG AGAAACATAA ACTTCATGAG CTGTGGCCTA TAGACTATCG AGTATGCCCT 
GTGCTGCCTT CCCCTCAGGT TTACAAATGC AATTTTAGGA CCTGTTTGCA TATGGCCACT 
TGGAAAGTTG GAAAATGGAG CAAGTGCTCT GTGACTTGTG GAGTTGGGAT GATGGAGAGA 
AGAGTGGAAT GCATGGCCGA TAATGGTCTG TCCAGTGATT TATGTTTAAA GCATTTAAAA 
CCAGATGCTC AAAAGAAATG TTATGTCAGT GACTGTAAAA CATTTATGAG CTGTAAAGAA 
ATACAAGTGA AAAACAACAT TACCAAGGAT GGTGACTATT ACCTTAACAT TAAAGGAAGA 
ATAACAAAGA TCTATTGTGC TGGCATGCAC TTGGAGAACC CTAAGGAATA TATATCATTG 
GTCAAAGGTG AAGAAGACAA CTTTTCTGAA GTGTATGGTT TTAGACTACG AAATCCATAT 
GAATGTCCTT TTAATGGAAG CAGGAGGCAT GACTGTGAAT GTAAAAATGA CTATCTGGCT 
GCTGGACACA CTGTTTTTAG CAAAATAAGA ATTGATCTTA TTTCCATGCA AATTAAAACT 
ACAGACCTTC TTTTTGCCCA CACAATATTT GGAAATGCAG TTCCATTTGC CACAGCTGGA 
GATTGCTATA GTGCTGCCAG ATGCCCACAG GGACAGTTCA GCATTAATTT GGCAGGAACT 
GGGATGAAGA TATCCAGTAC AGCAAAGTGG CTGGCTCAGG GCAGTTATGC CTCTGTGATC 
ATTCACAGAT CACAGGATGG AACCAAAGTC TATGGCAGAT GTGGAGGGTT CTGTGGAAAG 
TGTATTCCTC ACATGACTAC TGGTCTCCCA ATTCAAGTAG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ADAMTS20 ( XM_004006435.4 ) cDNA ORF clone, Ovis aries musimon(Ovis orientalis musimon) -> XP_004006484.2 Ovis aries ADAM metallopeptidase with thrombospondin type 1 motif 20 (ADAMTS20), transcript variant X1, mRNA.

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CloneID	OSm77935
Clone ID Related Accession (Same CDS sequence)	XM_004006435.4
Accession Version	XM_004006435.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5748bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-05
Organism	Ovis aries(sheep)
Product	A disintegrin and metalloproteinase with thrombospondin motifs 20 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040254.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Feb 6, 2019 this sequence version replaced XM_004006435.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ovis aries Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGGTGG TCAAGTGGCT GACGGGGCTG CTCTACCAGC TCTCGCTCTT CATCACCAGG 
TCTTGGGAAG TTCACTTCCA CCCCAGGCAG GAAGCCTTAG TGAGGACACT GGCCTCCTAC 
GAAGTGGTGA CCCCCGCGCG GGTCAATGAG TTCGGCGAAG TATTCCCGCA GAGCCACCAC 
TTCAGCCGGA GGAAGCGCAG CTCGGAGACT CTGGAGCCCA CACCGTTCAG AACCCACTAC 
CGAATCCGCG CCTACGGGCA GCTCTTCCAA CTGAACCTGA GCGCCGACGC GGCCTTCCTG 
GCTGCGGGAT ACACCGAGGT GCACTTAGGC ACCTCTGCGC ACCCGGCGGA GGAGCGCAGC 
GCGGCGCCCG CAGACCTGCG CCACTGCTTC TACCGCGGCC AGGTCAATGC TCGCGAGGAT 
CACACAGCCG TCTTCAGCCT CTGTGGAGGC TTGATGGGAA CATTTAAAGC ACATGATGGT 
GAATATTTCT TAGAACCTAT AATGAAGGCA GATGGGAGTG AACATGAAGA TGATCATAGC 
AAGCCACATC TTATATACAG ACAGGAATTA AAGGGGAACT ATTTTCTGCA GTCTCACAAG 
CCTTGTGATG CTTCAGAAAG TGAAATAAAG AAAACTGCTC TACCCTTTCA TAACTACAGC 
GACATTAATG AAAATCTCAA TATAAAGGAA GAAAGAGTTT TAGGATACAC TTCAAAAAAT 
ATATCTTTGG AAGATGAAAG AAGTCAATTG CATTCCAGGA AGAAACGTTT TTTATCATAT 
CCAAGATACG TGGAAGTTAT GGTTACAGCT GACTCTAAAA TGGTTCATCA TCATGGACAG 
AACTTGCAGC ACTACGTACT AACTCTGATG TCAATTGTTG CAGCAATCTA CAAGGACTCA 
AGTATTGGAA ATCTTATAAA CATAGTAATT GTAAAATTAG TTATAATTCA TGATGAGCAG 
GAAGGACCAG TCATCAGTTT TAATGCAGCT ACCACGTTAC GCAACTTCTG TTTGTGGCAA 
CAAACTCAGA ATGTTCTTGA CGATGCCCAC CCATCCCACC ATGATACTGC TGTTCTTATC 
ACAAGGGAAG ACATTTGTGG AGCTAGAGAG AAATGTGACA CATTAGGTTT AGCAGAATTA 
GGTACCCTGT GTGACCCTTT ACGGAGCTGC TCTATTAGTG AAGAAAATGG GCTCAGTGCT 
GCCTTTACCA TAGCCCATGA GCTTGGTCAT GTATTTAATG TTCCACACGA TGATAGTTTT 
AAATGTAAGG AAACTGGAAT TAAACATCAG TATCATGTCA TGGCTCCAAC TTTAAATTAC 
CACACAAGTC CTTGGACCTG GTCAAAATGT AGTCAGAAAT ATATCACTGA ATTCCTAGAC 
ACCGGTCATG GGGAATGCCT CCTTGACAAA CCAAATGGAA GAATATACGA TTTGTCTTCA 
CAGCTTCCTG GATTAATGTA TGATGTAAAC AAGCAATGTG AGCTTATGTT TGGTCCTGGA 
TCACAAGTGT GTCCCTATTT AAAACAATGC AGGCGTCTCT GGTGTACCAG CGCAGAAGGA 
GTCCACAAGG GCTGTCGCAC TCAACACATG CCTCTGGCAG ACGGAACAAA CTGTGGTCCT 
GGGATGCATT GCCACCATGG GCTATGTGTA AATAAAGAAA TGGAGACACG TCCTGTAGAT 
GGAGATTGGG GACCATGGGG ACCTTACAGT TCTTGTTCAA GAACATGTGG AGGTGGAATC 
AAAAGCACAA GCAGGCTCTG TAATCGCCCC GAGCCAAGAA ACGGAGGCAA GTACTGTGTG 
GGCCGCAGGA TGAAATTTCG ATCATGTAAC ACTGATTCAT GTCCAAAAGG CAAGCAAGAC 
TTCCGTGAGA AGCAGTGCTC TGATTTTGAT GGTAAACATT TCAACATCAA TGGTCTCTCC 
CCTAACGTGA GGTGGCTTCC AAAGTACAGT GGGATTGCTG TAAAAGATCG TTGTAAACTC 
TATTGTCGGG TGGCTGGAAC CACTAGCTTC TACCAACTGA AGGACAGGGT TGCCGATGGT 
ACTCCTTGTG GAACGGAAAC AAATGACATC TGTGTTCAAG GCCTGTGTCG GCAAGCTGGC 
TGTGATCACG TGTTAAACTC CAAGGCCAGG AGAGACAAAT GTGGCGTGTG TGGTGGGGAT 
AACTCCTCCT GCAGGACAAT GGCAGGCGTC TTCAACAGTG CGCATTATGG TTATAATGTC 
GTTGTAAAGA TTCCCCCAGG AGCAACAAAC ATTGATATTC TTCAGCACAG CTATTCTGGA 
AAACCAGAAG ATGACAATTA CCTGGCATTA TCGGACACTC AAGGGAATTT TCTTTTGAAT 
GGGAATTTTG TTGTAAGTAT GTCCAAACGG GAAATCAACG TACAAGGAGC TATTTTTGAA 
TACAGTGGTT CAAACAACTC AATTGAAAGG ATTAATAGTA CTGATCGACT GGAAGAAGAA 
CTTGTTTTGC AGGTTTTGTG TGTGGGTAAT CTATACAACC CTGATGTACG TTATTCCTTC 
AATATCCCCG TGGAAGAGAA GAGTGATCTA TTCACATGGG ACCCGTTTGG ACCATGGCAA 
GACTGTACAA AAATGTGTCA AGGTCTTCAT AGAAGAAAAA TTACTTGTGT ACGTAAGAGT 
GATCATATGG TCATGTCTGA TCAAAGCTGT GACCACTTAC CACTTCCATT GCTGGTAACT 
GAAAGGTGCA ATACAGACTG TGAACTCAGG TGGCACATCA TTGGCAAAAG TGAATGTTCA 
ACCCTTTGTG GTCAAGGATA CAAGTCATTG GACGTCCATT GTATGAAGTA TTCCATTCAT 
AAAGGACAGA CTGTTCCAGT AGATGACCAC TACTGCAGTG ACCAACTTAA ACCTCCAACC 
CGAGAACCCT GCCATGGTGC CTGTGTCTTA ACAAGGTGGC ATTATTTAGA ATGGTCTCAG 
TGTTCCAGGA GTTGCGGAGG AGGGGAGAGG TCTCGAGAAT CTTACTGTAT AAATAACTTT 
GGCCACCGTC TTGCTGACAG AGAATGCCAG GAACTTCCCC GAGTGACAAC AGAGAATTGC 
AATGAATTTT CCTGTCCCAG TTGGGCCACT AGTGAGTGGA GTGAGTGTCT GGTTACCTGT 
GGAAAAGGAA CAAAGCAGCG GCAGGTGTGG TGTCACCTGA ATGAAGATCA CTTGAGTGAT 
GGCTTCTGTG ACCCAAGCAC CAAGCCTGAG TCTCAGAGAC CCTGTGAGCT TCATGCATGT 
ACTTCCTGGC AAGTAGGACC TTGGGGTTCT TGCACAGCCA CGTGTGGACA GGGGTACCAG 
ATGCGTGCTG TGAAGTGTGT CAACGAGCTA TTTAGCACAG TGCTAGATGA CAGACTGTGC 
CATGGAGCTA GTCGCCCAAG TGAAAGGCAG AATTGTATTG TTACACCTTG CCCAGTTATC 
CCTAAAATTG GGGCCACTTC CTTGCCAGCT GTTCCCATGA GAAAGATAAC ACAATGGCGA 
TATGGTTCTT GGACACCATG CTCCGTGTCT TGTGGAAGAG GAAATCAAGC CCGCTATGTA 
AGTTGTCGTG ATGCTCATGA TGGAATAGCA GATGAATCAT TTTGTGCCCA CTTACCCCGA 
CCGGCTGAAA TAGCTGTCTG TTTTAGCCCT TGTGGAGAGT GGCAAACAGG GAATTGGTCA 
CCTTGTTCAG CTTCCTGTGG CCGTGGAAAA ATGACTCGAC AAGTTTTATG CATCAACTAT 
CATCAGCCAG TTAATGAGAA TTACTGTGAC CCTGAAGTCC GCCCTGTGAT AGAACAAGAA 
TGTAATCTGG CAGCCTGCCC TCCCACGTAC AGCCACTTTC CGAGTTCCTC TGAGCAGCCA 
AGCCATTTTC CAGGCAGGAA TTTTCCATTA ACTCACAAAC CAGAAGATAA TCAAAATCAG 
AGGGTCCATC CATCAATCAG AGAAAACCAG TGGAGAACAG GACCATGGGG ATCATGCTCT 
AGCAGTTGTG CTGGAGGTGT TCAGCGTAGA GTTGTGGTCT GCCAGGATGA AAATGGACAA 
AGTGCCAGTT ACTGTGATGC AGCGTCCAAA CCCCCAGAGT CAAAGCACTG TGATTCAGGA 
CCTTGTCCAC AGTGGAACTA TGGAAGCTGG GGAGAATGTA CGCAAACGTG TGGAGGAGGA 
ATAAAATCAA GATTTGTAAT ATGCCAGTTT CCCGATGGCC AGCTGTCACA AGAGCAAAAC 
TGTGAAATGT TGAACAAGCC GCCTAGTGTG GTACAGTGTC ATATACATGC TTGCCCTGAT 
GACGTATCAT GGCATCGGGG ACCATGGAAA TCGTGCTCAG CTTCCTGTGG TAAAGGTTTA 
AAATACCGTG AGGTGCTTTG CATTGACCAA TTCCACAGGA AATTAGAAGA AAAATATTGC 
AGTCATCTAC AGAAACCTCG AACACATAAA GCTTGTAGAT CTGTCCGATG CCCTTCATGG 
AAAGCCAACA GATGGAAGGA GTGCTCTGTG ACCTGCGGCC CCGGAGTTCA GCGGAGGGAT 
GTGTACTGCA GACTCAGAGG AGTTGGTCCG GTGGCGGAAG AAAAGTGTGA TCCATCCACG 
CGGCCTTATT TTCAGAGAGA GTGTTGGCAT CGGGACTGCA TACAGTACCA CTGGGCGGCA 
GGAGAGTGGT TGGATTGCTC AGTATCGTGT AAGAAAAGAG AGACATATCG GCAAGTGAAG 
TGCATGGATG CACAAAACAT TCAAGTGAGT GAGAGTTTCT GTGACCTCGC GACCAGACCT 
CTCGCGGTGA AAAAGTGCAG AAACTCTCCC TGCAAGTATA TCGTGGTAAC AGGAGACTCA 
TCCCAGTGTG CAGGTAACTG TGGATTTAGT TATCGACAAA GGATTACATA TTGCGTTGGG 
GTCCAATCTA TGGAGAAACA TAAACTTCAT GAGCTGTGGC CTATAGACTA TCGAGTATGC 
CCTGTGCTGC CTTCCCCTCA GGTTTACAAA TGCAATTTTA GGACCTGTTT GCATATGGCC 
ACTTGGAAAG TTGGAAAATG GAGCAAGTGC TCTGTGACTT GTGGAGTTGG GATGATGGAG 
AGAAGAGTGG AATGCATGGC CGATAATGGT CTGTCCAGTG ATTTATGTTT AAAGCATTTA 
AAACCAGATG CTCAAAAGAA ATGTTATGTC AGTGACTGTA AAACATTTAT GAGCTGTAAA 
GAAATACAAG TGAAAAACAA CATTACCAAG GATGGTGACT ATTACCTTAA CATTAAAGGA 
AGAATAACAA AGATCTATTG TGCTGGCATG CACTTGGAGA ACCCTAAGGA ATATATATCA 
TTGGTCAAAG GTGAAGAAGA CAACTTTTCT GAAGTGTATG GTTTTAGACT ACGAAATCCA 
TATGAATGTC CTTTTAATGG AAGCAGGAGG CATGACTGTG AATGTAAAAA TGACTATCTG 
GCTGCTGGAC ACACTGTTTT TAGCAAAATA AGAATTGATC TTATTTCCAT GCAAATTAAA 
ACTACAGACC TTCTTTTTGC CCAGACAATA TTTGGAAATG CAGTTCCATT TGCCACAGCT 
GGAGATTGCT ATAGTGCTGC CAGATGCCCA CAGGGACAGT TCAGCATTAA TTTGGCAGGA 
ACTGGGATGA AGATATCCAG TACAGCAAAG TGGCTGGCTC AGGGCAGTTA TGCCTCTGTG 
ATCATTCACA GATCACAGGA TGGAACCAAA GTCTATGGCA GATGTGGAGG GTTCTGTGGA 
AAGTGTATTC CTCACATGAC TACTGGTCTC CCAATTCAAG TAGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004006484.2
CDS	36..5783
Translation	MRVVKWLTGLLYQLSLFITRSWEVHFHPRQEALVRTLASYEVVTPARVNEFGEVFPQSHHFSRRKRSSETLEPTPFRTHYRIRAYGQLFQLNLSADAAFLAAGYTEVHLGTSAHPAEERSAAPADLRHCFYRGQVNAREDHTAVFSLCGGLMGTFKAHDGEYFLEPIMKADGSEHEDDHSKPHLIYRQELKGNYFLQSHKPCDASESEIKKTALPFHNYSDINENLNIKEERVLGYTSKNISLEDERSQLHSRKKRFLSYPRYVEVMVTADSKMVHHHGQNLQHYVLTLMSIVAAIYKDSSIGNLINIVIVKLVIIHDEQEGPVISFNAATTLRNFCLWQQTQNVLDDAHPSHHDTAVLITREDICGAREKCDTLGLAELGTLCDPLRSCSISEENGLSAAFTIAHELGHVFNVPHDDSFKCKETGIKHQYHVMAPTLNYHTSPWTWSKCSQKYITEFLDTGHGECLLDKPNGRIYDLSSQLPGLMYDVNKQCELMFGPGSQVCPYLKQCRRLWCTSAEGVHKGCRTQHMPLADGTNCGPGMHCHHGLCVNKEMETRPVDGDWGPWGPYSSCSRTCGGGIKSTSRLCNRPEPRNGGKYCVGRRMKFRSCNTDSCPKGKQDFREKQCSDFDGKHFNINGLSPNVRWLPKYSGIAVKDRCKLYCRVAGTTSFYQLKDRVADGTPCGTETNDICVQGLCRQAGCDHVLNSKARRDKCGVCGGDNSSCRTMAGVFNSAHYGYNVVVKIPPGATNIDILQHSYSGKPEDDNYLALSDTQGNFLLNGNFVVSMSKREINVQGAIFEYSGSNNSIERINSTDRLEEELVLQVLCVGNLYNPDVRYSFNIPVEEKSDLFTWDPFGPWQDCTKMCQGLHRRKITCVRKSDHMVMSDQSCDHLPLPLLVTERCNTDCELRWHIIGKSECSTLCGQGYKSLDVHCMKYSIHKGQTVPVDDHYCSDQLKPPTREPCHGACVLTRWHYLEWSQCSRSCGGGERSRESYCINNFGHRLADRECQELPRVTTENCNEFSCPSWATSEWSECLVTCGKGTKQRQVWCHLNEDHLSDGFCDPSTKPESQRPCELHACTSWQVGPWGSCTATCGQGYQMRAVKCVNELFSTVLDDRLCHGASRPSERQNCIVTPCPVIPKIGATSLPAVPMRKITQWRYGSWTPCSVSCGRGNQARYVSCRDAHDGIADESFCAHLPRPAEIAVCFSPCGEWQTGNWSPCSASCGRGKMTRQVLCINYHQPVNENYCDPEVRPVIEQECNLAACPPTYSHFPSSSEQPSHFPGRNFPLTHKPEDNQNQRVHPSIRENQWRTGPWGSCSSSCAGGVQRRVVVCQDENGQSASYCDAASKPPESKHCDSGPCPQWNYGSWGECTQTCGGGIKSRFVICQFPDGQLSQEQNCEMLNKPPSVVQCHIHACPDDVSWHRGPWKSCSASCGKGLKYREVLCIDQFHRKLEEKYCSHLQKPRTHKACRSVRCPSWKANRWKECSVTCGPGVQRRDVYCRLRGVGPVAEEKCDPSTRPYFQRECWHRDCIQYHWAAGEWLDCSVSCKKRETYRQVKCMDAQNIQVSESFCDLATRPLAVKKCRNSPCKYIVVTGDSSQCAGNCGFSYRQRITYCVGVQSMEKHKLHELWPIDYRVCPVLPSPQVYKCNFRTCLHMATWKVGKWSKCSVTCGVGMMERRVECMADNGLSSDLCLKHLKPDAQKKCYVSDCKTFMSCKEIQVKNNITKDGDYYLNIKGRITKIYCAGMHLENPKEYISLVKGEEDNFSEVYGFRLRNPYECPFNGSRRHDCECKNDYLAAGHTVFSKIRIDLISMQIKTTDLLFAQTIFGNAVPFATAGDCYSAARCPQGQFSINLAGTGMKISSTAKWLAQGSYASVIIHRSQDGTKVYGRCGGFCGKCIPHMTTGLPIQVV

Target ORF information:

RefSeq Version	XM_004006435.4
Organism	Ovis aries(sheep)
Definition	Ovis aries ADAM metallopeptidase with thrombospondin type 1 motif 20 (ADAMTS20), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004006435.4

ORF Insert Sequence:

ATGCGGGTGG TCAAGTGGCT GACGGGGCTG CTCTACCAGC TCTCGCTCTT CATCACCAGG 
TCTTGGGAAG TTCACTTCCA CCCCAGGCAG GAAGCCTTAG TGAGGACACT GGCCTCCTAC 
GAAGTGGTGA CCCCCGCGCG GGTCAATGAG TTCGGCGAAG TATTCCCGCA GAGCCACCAC 
TTCAGCCGGA GGAAGCGCAG CTCGGAGACT CTGGAGCCCA CACCGTTCAG AACCCACTAC 
CGAATCCGCG CCTACGGGCA GCTCTTCCAA CTGAACCTGA GCGCCGACGC GGCCTTCCTG 
GCTGCGGGAT ACACCGAGGT GCACTTAGGC ACCTCTGCGC ACCCGGCGGA GGAGCGCAGC 
GCGGCGCCCG CAGACCTGCG CCACTGCTTC TACCGCGGCC AGGTCAATGC TCGCGAGGAT 
CACACAGCCG TCTTCAGCCT CTGTGGAGGC TTGATGGGAA CATTTAAAGC ACATGATGGT 
GAATATTTCT TAGAACCTAT AATGAAGGCA GATGGGAGTG AACATGAAGA TGATCATAGC 
AAGCCACATC TTATATACAG ACAGGAATTA AAGGGGAACT ATTTTCTGCA GTCTCACAAG 
CCTTGTGATG CTTCAGAAAG TGAAATAAAG AAAACTGCTC TACCCTTTCA TAACTACAGC 
GACATTAATG AAAATCTCAA TATAAAGGAA GAAAGAGTTT TAGGATACAC TTCAAAAAAT 
ATATCTTTGG AAGATGAAAG AAGTCAATTG CATTCCAGGA AGAAACGTTT TTTATCATAT 
CCAAGATACG TGGAAGTTAT GGTTACAGCT GACTCTAAAA TGGTTCATCA TCATGGACAG 
AACTTGCAGC ACTACGTACT AACTCTGATG TCAATTGTTG CAGCAATCTA CAAGGACTCA 
AGTATTGGAA ATCTTATAAA CATAGTAATT GTAAAATTAG TTATAATTCA TGATGAGCAG 
GAAGGACCAG TCATCAGTTT TAATGCAGCT ACCACGTTAC GCAACTTCTG TTTGTGGCAA 
CAAACTCAGA ATGTTCTTGA CGATGCCCAC CCATCCCACC ATGATACTGC TGTTCTTATC 
ACAAGGGAAG ACATTTGTGG AGCTAGAGAG AAATGTGACA CATTAGGTTT AGCAGAATTA 
GGTACCCTGT GTGACCCTTT ACGGAGCTGC TCTATTAGTG AAGAAAATGG GCTCAGTGCT 
GCCTTTACCA TAGCCCATGA GCTTGGTCAT GTATTTAATG TTCCACACGA TGATAGTTTT 
AAATGTAAGG AAACTGGAAT TAAACATCAG TATCATGTCA TGGCTCCAAC TTTAAATTAC 
CACACAAGTC CTTGGACCTG GTCAAAATGT AGTCAGAAAT ATATCACTGA ATTCCTAGAC 
ACCGGTCATG GGGAATGCCT CCTTGACAAA CCAAATGGAA GAATATACGA TTTGTCTTCA 
CAGCTTCCTG GATTAATGTA TGATGTAAAC AAGCAATGTG AGCTTATGTT TGGTCCTGGA 
TCACAAGTGT GTCCCTATTT AAAACAATGC AGGCGTCTCT GGTGTACCAG CGCAGAAGGA 
GTCCACAAGG GCTGTCGCAC TCAACACATG CCTCTGGCAG ACGGAACAAA CTGTGGTCCT 
GGGATGCATT GCCACCATGG GCTATGTGTA AATAAAGAAA TGGAGACACG TCCTGTAGAT 
GGAGATTGGG GACCATGGGG ACCTTACAGT TCTTGTTCAA GAACATGTGG AGGTGGAATC 
AAAAGCACAA GCAGGCTCTG TAATCGCCCC GAGCCAAGAA ACGGAGGCAA GTACTGTGTG 
GGCCGCAGGA TGAAATTTCG ATCATGTAAC ACTGATTCAT GTCCAAAAGG CAAGCAAGAC 
TTCCGTGAGA AGCAGTGCTC TGATTTTGAT GGTAAACATT TCAACATCAA TGGTCTCTCC 
CCTAACGTGA GGTGGCTTCC AAAGTACAGT GGGATTGCTG TAAAAGATCG TTGTAAACTC 
TATTGTCGGG TGGCTGGAAC CACTAGCTTC TACCAACTGA AGGACAGGGT TGCCGATGGT 
ACTCCTTGTG GAACGGAAAC AAATGACATC TGTGTTCAAG GCCTGTGTCG GCAAGCTGGC 
TGTGATCACG TGTTAAACTC CAAGGCCAGG AGAGACAAAT GTGGCGTGTG TGGTGGGGAT 
AACTCCTCCT GCAGGACAAT GGCAGGCGTC TTCAACAGTG CGCATTATGG TTATAATGTC 
GTTGTAAAGA TTCCCCCAGG AGCAACAAAC ATTGATATTC TTCAGCACAG CTATTCTGGA 
AAACCAGAAG ATGACAATTA CCTGGCATTA TCGGACACTC AAGGGAATTT TCTTTTGAAT 
GGGAATTTTG TTGTAAGTAT GTCCAAACGG GAAATCAACG TACAAGGAGC TATTTTTGAA 
TACAGTGGTT CAAACAACTC AATTGAAAGG ATTAATAGTA CTGATCGACT GGAAGAAGAA 
CTTGTTTTGC AGGTTTTGTG TGTGGGTAAT CTATACAACC CTGATGTACG TTATTCCTTC 
AATATCCCCG TGGAAGAGAA GAGTGATCTA TTCACATGGG ACCCGTTTGG ACCATGGCAA 
GACTGTACAA AAATGTGTCA AGGTCTTCAT AGAAGAAAAA TTACTTGTGT ACGTAAGAGT 
GATCATATGG TCATGTCTGA TCAAAGCTGT GACCACTTAC CACTTCCATT GCTGGTAACT 
GAAAGGTGCA ATACAGACTG TGAACTCAGG TGGCACATCA TTGGCAAAAG TGAATGTTCA 
ACCCTTTGTG GTCAAGGATA CAAGTCATTG GACGTCCATT GTATGAAGTA TTCCATTCAT 
AAAGGACAGA CTGTTCCAGT AGATGACCAC TACTGCAGTG ACCAACTTAA ACCTCCAACC 
CGAGAACCCT GCCATGGTGC CTGTGTCTTA ACAAGGTGGC ATTATTTAGA ATGGTCTCAG 
TGTTCCAGGA GTTGCGGAGG AGGGGAGAGG TCTCGAGAAT CTTACTGTAT AAATAACTTT 
GGCCACCGTC TTGCTGACAG AGAATGCCAG GAACTTCCCC GAGTGACAAC AGAGAATTGC 
AATGAATTTT CCTGTCCCAG TTGGGCCACT AGTGAGTGGA GTGAGTGTCT GGTTACCTGT 
GGAAAAGGAA CAAAGCAGCG GCAGGTGTGG TGTCACCTGA ATGAAGATCA CTTGAGTGAT 
GGCTTCTGTG ACCCAAGCAC CAAGCCTGAG TCTCAGAGAC CCTGTGAGCT TCATGCATGT 
ACTTCCTGGC AAGTAGGACC TTGGGGTTCT TGCACAGCCA CGTGTGGACA GGGGTACCAG 
ATGCGTGCTG TGAAGTGTGT CAACGAGCTA TTTAGCACAG TGCTAGATGA CAGACTGTGC 
CATGGAGCTA GTCGCCCAAG TGAAAGGCAG AATTGTATTG TTACACCTTG CCCAGTTATC 
CCTAAAATTG GGGCCACTTC CTTGCCAGCT GTTCCCATGA GAAAGATAAC ACAATGGCGA 
TATGGTTCTT GGACACCATG CTCCGTGTCT TGTGGAAGAG GAAATCAAGC CCGCTATGTA 
AGTTGTCGTG ATGCTCATGA TGGAATAGCA GATGAATCAT TTTGTGCCCA CTTACCCCGA 
CCGGCTGAAA TAGCTGTCTG TTTTAGCCCT TGTGGAGAGT GGCAAACAGG GAATTGGTCA 
CCTTGTTCAG CTTCCTGTGG CCGTGGAAAA ATGACTCGAC AAGTTTTATG CATCAACTAT 
CATCAGCCAG TTAATGAGAA TTACTGTGAC CCTGAAGTCC GCCCTGTGAT AGAACAAGAA 
TGTAATCTGG CAGCCTGCCC TCCCACGTAC AGCCACTTTC CGAGTTCCTC TGAGCAGCCA 
AGCCATTTTC CAGGCAGGAA TTTTCCATTA ACTCACAAAC CAGAAGATAA TCAAAATCAG 
AGGGTCCATC CATCAATCAG AGAAAACCAG TGGAGAACAG GACCATGGGG ATCATGCTCT 
AGCAGTTGTG CTGGAGGTGT TCAGCGTAGA GTTGTGGTCT GCCAGGATGA AAATGGACAA 
AGTGCCAGTT ACTGTGATGC AGCGTCCAAA CCCCCAGAGT CAAAGCACTG TGATTCAGGA 
CCTTGTCCAC AGTGGAACTA TGGAAGCTGG GGAGAATGTA CGCAAACGTG TGGAGGAGGA 
ATAAAATCAA GATTTGTAAT ATGCCAGTTT CCCGATGGCC AGCTGTCACA AGAGCAAAAC 
TGTGAAATGT TGAACAAGCC GCCTAGTGTG GTACAGTGTC ATATACATGC TTGCCCTGAT 
GACGTATCAT GGCATCGGGG ACCATGGAAA TCGTGCTCAG CTTCCTGTGG TAAAGGTTTA 
AAATACCGTG AGGTGCTTTG CATTGACCAA TTCCACAGGA AATTAGAAGA AAAATATTGC 
AGTCATCTAC AGAAACCTCG AACACATAAA GCTTGTAGAT CTGTCCGATG CCCTTCATGG 
AAAGCCAACA GATGGAAGGA GTGCTCTGTG ACCTGCGGCC CCGGAGTTCA GCGGAGGGAT 
GTGTACTGCA GACTCAGAGG AGTTGGTCCG GTGGCGGAAG AAAAGTGTGA TCCATCCACG 
CGGCCTTATT TTCAGAGAGA GTGTTGGCAT CGGGACTGCA TACAGTACCA CTGGGCGGCA 
GGAGAGTGGT TGGATTGCTC AGTATCGTGT AAGAAAAGAG AGACATATCG GCAAGTGAAG 
TGCATGGATG CACAAAACAT TCAAGTGAGT GAGAGTTTCT GTGACCTCGC GACCAGACCT 
CTCGCGGTGA AAAAGTGCAG AAACTCTCCC TGCAAGTATA TCGTGGTAAC AGGAGACTCA 
TCCCAGTGTG CAGGTAACTG TGGATTTAGT TATCGACAAA GGATTACATA TTGCGTTGGG 
GTCCAATCTA TGGAGAAACA TAAACTTCAT GAGCTGTGGC CTATAGACTA TCGAGTATGC 
CCTGTGCTGC CTTCCCCTCA GGTTTACAAA TGCAATTTTA GGACCTGTTT GCATATGGCC 
ACTTGGAAAG TTGGAAAATG GAGCAAGTGC TCTGTGACTT GTGGAGTTGG GATGATGGAG 
AGAAGAGTGG AATGCATGGC CGATAATGGT CTGTCCAGTG ATTTATGTTT AAAGCATTTA 
AAACCAGATG CTCAAAAGAA ATGTTATGTC AGTGACTGTA AAACATTTAT GAGCTGTAAA 
GAAATACAAG TGAAAAACAA CATTACCAAG GATGGTGACT ATTACCTTAA CATTAAAGGA 
AGAATAACAA AGATCTATTG TGCTGGCATG CACTTGGAGA ACCCTAAGGA ATATATATCA 
TTGGTCAAAG GTGAAGAAGA CAACTTTTCT GAAGTGTATG GTTTTAGACT ACGAAATCCA 
TATGAATGTC CTTTTAATGG AAGCAGGAGG CATGACTGTG AATGTAAAAA TGACTATCTG 
GCTGCTGGAC ACACTGTTTT TAGCAAAATA AGAATTGATC TTATTTCCAT GCAAATTAAA 
ACTACAGACC TTCTTTTTGC CCAGACAATA TTTGGAAATG CAGTTCCATT TGCCACAGCT 
GGAGATTGCT ATAGTGCTGC CAGATGCCCA CAGGGACAGT TCAGCATTAA TTTGGCAGGA 
ACTGGGATGA AGATATCCAG TACAGCAAAG TGGCTGGCTC AGGGCAGTTA TGCCTCTGTG 
ATCATTCACA GATCACAGGA TGGAACCAAA GTCTATGGCA GATGTGGAGG GTTCTGTGGA 
AAGTGTATTC CTCACATGAC TACTGGTCTC CCAATTCAAG TAGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ADAMTS20 ( XM_015094726.2 ) cDNA ORF clone, Ovis aries musimon(Ovis orientalis musimon) -> XP_014950212.2 Ovis aries ADAM metallopeptidase with thrombospondin type 1 motif 20 (ADAMTS20), transcript variant X2, mRNA.

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CloneID	OSm77936
Clone ID Related Accession (Same CDS sequence)	XM_015094726.2
Accession Version	XM_015094726.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4665bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-05
Organism	Ovis aries(sheep)
Product	A disintegrin and metalloproteinase with thrombospondin motifs 20 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040254.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Feb 6, 2019 this sequence version replaced XM_015094726.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ovis aries Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTTGG TCATGTGTCA GACAGGCTAC ACAGATCAAC AGACTAAGCA AAGAACTAGA 
AATTTGGAAC TCTTTTCTTT CATTTACTGT ATTGGATTAT ATGACAAGCA CTTCTGGCCT 
CTATCCCTTG TTATCCTATG CATGACCAGA TTTAATGTTC CACACGATGA TAGTTTTAAA 
TGTAAGGAAA CTGGAATTAA ACATCAGTAT CATGTCATGG CTCCAACTTT AAATTACCAC 
ACAAGTCCTT GGACCTGGTC AAAATGTAGT CAGAAATATA TCACTGAATT CCTAGACACC 
GGTCATGGGG AATGCCTCCT TGACAAACCA AATGGAAGAA TATACGATTT GTCTTCACAG 
CTTCCTGGAT TAATGTATGA TGTAAACAAG CAATGTGAGC TTATGTTTGG TCCTGGATCA 
CAAGTGTGTC CCTATTTAAA ACAATGCAGG CGTCTCTGGT GTACCAGCGC AGAAGGAGTC 
CACAAGGGCT GTCGCACTCA ACACATGCCT CTGGCAGACG GAACAAACTG TGGTCCTGGG 
ATGCATTGCC ACCATGGGCT ATGTGTAAAT AAAGAAATGG AGACACGTCC TGTAGATGGA 
GATTGGGGAC CATGGGGACC TTACAGTTCT TGTTCAAGAA CATGTGGAGG TGGAATCAAA 
AGCACAAGCA GGCTCTGTAA TCGCCCCGAG CCAAGAAACG GAGGCAAGTA CTGTGTGGGC 
CGCAGGATGA AATTTCGATC ATGTAACACT GATTCATGTC CAAAAGGCAA GCAAGACTTC 
CGTGAGAAGC AGTGCTCTGA TTTTGATGGT AAACATTTCA ACATCAATGG TCTCTCCCCT 
AACGTGAGGT GGCTTCCAAA GTACAGTGGG ATTGCTGTAA AAGATCGTTG TAAACTCTAT 
TGTCGGGTGG CTGGAACCAC TAGCTTCTAC CAACTGAAGG ACAGGGTTGC CGATGGTACT 
CCTTGTGGAA CGGAAACAAA TGACATCTGT GTTCAAGGCC TGTGTCGGCA AGCTGGCTGT 
GATCACGTGT TAAACTCCAA GGCCAGGAGA GACAAATGTG GCGTGTGTGG TGGGGATAAC 
TCCTCCTGCA GGACAATGGC AGGCGTCTTC AACAGTGCGC ATTATGGTTA TAATGTCGTT 
GTAAAGATTC CCCCAGGAGC AACAAACATT GATATTCTTC AGCACAGCTA TTCTGGAAAA 
CCAGAAGATG ACAATTACCT GGCATTATCG GACACTCAAG GGAATTTTCT TTTGAATGGG 
AATTTTGTTG TAAGTATGTC CAAACGGGAA ATCAACGTAC AAGGAGCTAT TTTTGAATAC 
AGTGGTTCAA ACAACTCAAT TGAAAGGATT AATAGTACTG ATCGACTGGA AGAAGAACTT 
GTTTTGCAGG TTTTGTGTGT GGGTAATCTA TACAACCCTG ATGTACGTTA TTCCTTCAAT 
ATCCCCGTGG AAGAGAAGAG TGATCTATTC ACATGGGACC CGTTTGGACC ATGGCAAGAC 
TGTACAAAAA TGTGTCAAGG TCTTCATAGA AGAAAAATTA CTTGTGTACG TAAGAGTGAT 
CATATGGTCA TGTCTGATCA AAGCTGTGAC CACTTACCAC TTCCATTGCT GGTAACTGAA 
AGGTGCAATA CAGACTGTGA ACTCAGGTGG CACATCATTG GCAAAAGTGA ATGTTCAACC 
CTTTGTGGTC AAGGATACAA GTCATTGGAC GTCCATTGTA TGAAGTATTC CATTCATAAA 
GGACAGACTG TTCCAGTAGA TGACCACTAC TGCAGTGACC AACTTAAACC TCCAACCCGA 
GAACCCTGCC ATGGTGCCTG TGTCTTAACA AGGTGGCATT ATTTAGAATG GTCTCAGTGT 
TCCAGGAGTT GCGGAGGAGG GGAGAGGTCT CGAGAATCTT ACTGTATAAA TAACTTTGGC 
CACCGTCTTG CTGACAGAGA ATGCCAGGAA CTTCCCCGAG TGACAACAGA GAATTGCAAT 
GAATTTTCCT GTCCCAGTTG GGCCACTAGT GAGTGGAGTG AGTGTCTGGT TACCTGTGGA 
AAAGGAACAA AGCAGCGGCA GGTGTGGTGT CACCTGAATG AAGATCACTT GAGTGATGGC 
TTCTGTGACC CAAGCACCAA GCCTGAGTCT CAGAGACCCT GTGAGCTTCA TGCATGTACT 
TCCTGGCAAG TAGGACCTTG GGGTTCTTGC ACAGCCACGT GTGGACAGGG GTACCAGATG 
CGTGCTGTGA AGTGTGTCAA CGAGCTATTT AGCACAGTGC TAGATGACAG ACTGTGCCAT 
GGAGCTAGTC GCCCAAGTGA AAGGCAGAAT TGTATTGTTA CACCTTGCCC AGTTATCCCT 
AAAATTGGGG CCACTTCCTT GCCAGCTGTT CCCATGAGAA AGATAACACA ATGGCGATAT 
GGTTCTTGGA CACCATGCTC CGTGTCTTGT GGAAGAGGAA ATCAAGCCCG CTATGTAAGT 
TGTCGTGATG CTCATGATGG AATAGCAGAT GAATCATTTT GTGCCCACTT ACCCCGACCG 
GCTGAAATAG CTGTCTGTTT TAGCCCTTGT GGAGAGTGGC AAACAGGGAA TTGGTCACCT 
TGTTCAGCTT CCTGTGGCCG TGGAAAAATG ACTCGACAAG TTTTATGCAT CAACTATCAT 
CAGCCAGTTA ATGAGAATTA CTGTGACCCT GAAGTCCGCC CTGTGATAGA ACAAGAATGT 
AATCTGGCAG CCTGCCCTCC CACGTACAGC CACTTTCCGA GTTCCTCTGA GCAGCCAAGC 
CATTTTCCAG GCAGGAATTT TCCATTAACT CACAAACCAG AAGATAATCA AAATCAGAGG 
GTCCATCCAT CAATCAGAGA AAACCAGTGG AGAACAGGAC CATGGGGATC ATGCTCTAGC 
AGTTGTGCTG GAGGTGTTCA GCGTAGAGTT GTGGTCTGCC AGGATGAAAA TGGACAAAGT 
GCCAGTTACT GTGATGCAGC GTCCAAACCC CCAGAGTCAA AGCACTGTGA TTCAGGACCT 
TGTCCACAGT GGAACTATGG AAGCTGGGGA GAATGTACGC AAACGTGTGG AGGAGGAATA 
AAATCAAGAT TTGTAATATG CCAGTTTCCC GATGGCCAGC TGTCACAAGA GCAAAACTGT 
GAAATGTTGA ACAAGCCGCC TAGTGTGGTA CAGTGTCATA TACATGCTTG CCCTGATGAC 
GTATCATGGC ATCGGGGACC ATGGAAATCG TGCTCAGCTT CCTGTGGTAA AGGTTTAAAA 
TACCGTGAGG TGCTTTGCAT TGACCAATTC CACAGGAAAT TAGAAGAAAA ATATTGCAGT 
CATCTACAGA AACCTCGAAC ACATAAAGCT TGTAGATCTG TCCGATGCCC TTCATGGAAA 
GCCAACAGAT GGAAGGAGTG CTCTGTGACC TGCGGCCCCG GAGTTCAGCG GAGGGATGTG 
TACTGCAGAC TCAGAGGAGT TGGTCCGGTG GCGGAAGAAA AGTGTGATCC ATCCACGCGG 
CCTTATTTTC AGAGAGAGTG TTGGCATCGG GACTGCATAC AGTACCACTG GGCGGCAGGA 
GAGTGGTTGG ATTGCTCAGT ATCGTGTAAG AAAAGAGAGA CATATCGGCA AGTGAAGTGC 
ATGGATGCAC AAAACATTCA AGTGAGTGAG AGTTTCTGTG ACCTCGCGAC CAGACCTCTC 
GCGGTGAAAA AGTGCAGAAA CTCTCCCTGC AAGTATATCG TGGTAACAGG AGACTCATCC 
CAGTGTGCAG GTAACTGTGG ATTTAGTTAT CGACAAAGGA TTACATATTG CGTTGGGGTC 
CAATCTATGG AGAAACATAA ACTTCATGAG CTGTGGCCTA TAGACTATCG AGTATGCCCT 
GTGCTGCCTT CCCCTCAGGT TTACAAATGC AATTTTAGGA CCTGTTTGCA TATGGCCACT 
TGGAAAGTTG GAAAATGGAG CAAGTGCTCT GTGACTTGTG GAGTTGGGAT GATGGAGAGA 
AGAGTGGAAT GCATGGCCGA TAATGGTCTG TCCAGTGATT TATGTTTAAA GCATTTAAAA 
CCAGATGCTC AAAAGAAATG TTATGTCAGT GACTGTAAAA CATTTATGAG CTGTAAAGAA 
ATACAAGTGA AAAACAACAT TACCAAGGAT GGTGACTATT ACCTTAACAT TAAAGGAAGA 
ATAACAAAGA TCTATTGTGC TGGCATGCAC TTGGAGAACC CTAAGGAATA TATATCATTG 
GTCAAAGGTG AAGAAGACAA CTTTTCTGAA GTGTATGGTT TTAGACTACG AAATCCATAT 
GAATGTCCTT TTAATGGAAG CAGGAGGCAT GACTGTGAAT GTAAAAATGA CTATCTGGCT 
GCTGGACACA CTGTTTTTAG CAAAATAAGA ATTGATCTTA TTTCCATGCA AATTAAAACT 
ACAGACCTTC TTTTTGCCCA GACAATATTT GGAAATGCAG TTCCATTTGC CACAGCTGGA 
GATTGCTATA GTGCTGCCAG ATGCCCACAG GGACAGTTCA GCATTAATTT GGCAGGAACT 
GGGATGAAGA TATCCAGTAC AGCAAAGTGG CTGGCTCAGG GCAGTTATGC CTCTGTGATC 
ATTCACAGAT CACAGGATGG AACCAAAGTC TATGGCAGAT GTGGAGGGTT CTGTGGAAAG 
TGTATTCCTC ACATGACTAC TGGTCTCCCA ATTCAAGTAG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014950212.2
CDS	1620..6284
Translation	MSLVMCQTGYTDQQTKQRTRNLELFSFIYCIGLYDKHFWPLSLVILCMTRFNVPHDDSFKCKETGIKHQYHVMAPTLNYHTSPWTWSKCSQKYITEFLDTGHGECLLDKPNGRIYDLSSQLPGLMYDVNKQCELMFGPGSQVCPYLKQCRRLWCTSAEGVHKGCRTQHMPLADGTNCGPGMHCHHGLCVNKEMETRPVDGDWGPWGPYSSCSRTCGGGIKSTSRLCNRPEPRNGGKYCVGRRMKFRSCNTDSCPKGKQDFREKQCSDFDGKHFNINGLSPNVRWLPKYSGIAVKDRCKLYCRVAGTTSFYQLKDRVADGTPCGTETNDICVQGLCRQAGCDHVLNSKARRDKCGVCGGDNSSCRTMAGVFNSAHYGYNVVVKIPPGATNIDILQHSYSGKPEDDNYLALSDTQGNFLLNGNFVVSMSKREINVQGAIFEYSGSNNSIERINSTDRLEEELVLQVLCVGNLYNPDVRYSFNIPVEEKSDLFTWDPFGPWQDCTKMCQGLHRRKITCVRKSDHMVMSDQSCDHLPLPLLVTERCNTDCELRWHIIGKSECSTLCGQGYKSLDVHCMKYSIHKGQTVPVDDHYCSDQLKPPTREPCHGACVLTRWHYLEWSQCSRSCGGGERSRESYCINNFGHRLADRECQELPRVTTENCNEFSCPSWATSEWSECLVTCGKGTKQRQVWCHLNEDHLSDGFCDPSTKPESQRPCELHACTSWQVGPWGSCTATCGQGYQMRAVKCVNELFSTVLDDRLCHGASRPSERQNCIVTPCPVIPKIGATSLPAVPMRKITQWRYGSWTPCSVSCGRGNQARYVSCRDAHDGIADESFCAHLPRPAEIAVCFSPCGEWQTGNWSPCSASCGRGKMTRQVLCINYHQPVNENYCDPEVRPVIEQECNLAACPPTYSHFPSSSEQPSHFPGRNFPLTHKPEDNQNQRVHPSIRENQWRTGPWGSCSSSCAGGVQRRVVVCQDENGQSASYCDAASKPPESKHCDSGPCPQWNYGSWGECTQTCGGGIKSRFVICQFPDGQLSQEQNCEMLNKPPSVVQCHIHACPDDVSWHRGPWKSCSASCGKGLKYREVLCIDQFHRKLEEKYCSHLQKPRTHKACRSVRCPSWKANRWKECSVTCGPGVQRRDVYCRLRGVGPVAEEKCDPSTRPYFQRECWHRDCIQYHWAAGEWLDCSVSCKKRETYRQVKCMDAQNIQVSESFCDLATRPLAVKKCRNSPCKYIVVTGDSSQCAGNCGFSYRQRITYCVGVQSMEKHKLHELWPIDYRVCPVLPSPQVYKCNFRTCLHMATWKVGKWSKCSVTCGVGMMERRVECMADNGLSSDLCLKHLKPDAQKKCYVSDCKTFMSCKEIQVKNNITKDGDYYLNIKGRITKIYCAGMHLENPKEYISLVKGEEDNFSEVYGFRLRNPYECPFNGSRRHDCECKNDYLAAGHTVFSKIRIDLISMQIKTTDLLFAQTIFGNAVPFATAGDCYSAARCPQGQFSINLAGTGMKISSTAKWLAQGSYASVIIHRSQDGTKVYGRCGGFCGKCIPHMTTGLPIQVV

Target ORF information:

RefSeq Version	XM_015094726.2
Organism	Ovis aries(sheep)
Definition	Ovis aries ADAM metallopeptidase with thrombospondin type 1 motif 20 (ADAMTS20), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015094726.2

ORF Insert Sequence:

ATGAGCTTGG TCATGTGTCA GACAGGCTAC ACAGATCAAC AGACTAAGCA AAGAACTAGA 
AATTTGGAAC TCTTTTCTTT CATTTACTGT ATTGGATTAT ATGACAAGCA CTTCTGGCCT 
CTATCCCTTG TTATCCTATG CATGACCAGA TTTAATGTTC CACACGATGA TAGTTTTAAA 
TGTAAGGAAA CTGGAATTAA ACATCAGTAT CATGTCATGG CTCCAACTTT AAATTACCAC 
ACAAGTCCTT GGACCTGGTC AAAATGTAGT CAGAAATATA TCACTGAATT CCTAGACACC 
GGTCATGGGG AATGCCTCCT TGACAAACCA AATGGAAGAA TATACGATTT GTCTTCACAG 
CTTCCTGGAT TAATGTATGA TGTAAACAAG CAATGTGAGC TTATGTTTGG TCCTGGATCA 
CAAGTGTGTC CCTATTTAAA ACAATGCAGG CGTCTCTGGT GTACCAGCGC AGAAGGAGTC 
CACAAGGGCT GTCGCACTCA ACACATGCCT CTGGCAGACG GAACAAACTG TGGTCCTGGG 
ATGCATTGCC ACCATGGGCT ATGTGTAAAT AAAGAAATGG AGACACGTCC TGTAGATGGA 
GATTGGGGAC CATGGGGACC TTACAGTTCT TGTTCAAGAA CATGTGGAGG TGGAATCAAA 
AGCACAAGCA GGCTCTGTAA TCGCCCCGAG CCAAGAAACG GAGGCAAGTA CTGTGTGGGC 
CGCAGGATGA AATTTCGATC ATGTAACACT GATTCATGTC CAAAAGGCAA GCAAGACTTC 
CGTGAGAAGC AGTGCTCTGA TTTTGATGGT AAACATTTCA ACATCAATGG TCTCTCCCCT 
AACGTGAGGT GGCTTCCAAA GTACAGTGGG ATTGCTGTAA AAGATCGTTG TAAACTCTAT 
TGTCGGGTGG CTGGAACCAC TAGCTTCTAC CAACTGAAGG ACAGGGTTGC CGATGGTACT 
CCTTGTGGAA CGGAAACAAA TGACATCTGT GTTCAAGGCC TGTGTCGGCA AGCTGGCTGT 
GATCACGTGT TAAACTCCAA GGCCAGGAGA GACAAATGTG GCGTGTGTGG TGGGGATAAC 
TCCTCCTGCA GGACAATGGC AGGCGTCTTC AACAGTGCGC ATTATGGTTA TAATGTCGTT 
GTAAAGATTC CCCCAGGAGC AACAAACATT GATATTCTTC AGCACAGCTA TTCTGGAAAA 
CCAGAAGATG ACAATTACCT GGCATTATCG GACACTCAAG GGAATTTTCT TTTGAATGGG 
AATTTTGTTG TAAGTATGTC CAAACGGGAA ATCAACGTAC AAGGAGCTAT TTTTGAATAC 
AGTGGTTCAA ACAACTCAAT TGAAAGGATT AATAGTACTG ATCGACTGGA AGAAGAACTT 
GTTTTGCAGG TTTTGTGTGT GGGTAATCTA TACAACCCTG ATGTACGTTA TTCCTTCAAT 
ATCCCCGTGG AAGAGAAGAG TGATCTATTC ACATGGGACC CGTTTGGACC ATGGCAAGAC 
TGTACAAAAA TGTGTCAAGG TCTTCATAGA AGAAAAATTA CTTGTGTACG TAAGAGTGAT 
CATATGGTCA TGTCTGATCA AAGCTGTGAC CACTTACCAC TTCCATTGCT GGTAACTGAA 
AGGTGCAATA CAGACTGTGA ACTCAGGTGG CACATCATTG GCAAAAGTGA ATGTTCAACC 
CTTTGTGGTC AAGGATACAA GTCATTGGAC GTCCATTGTA TGAAGTATTC CATTCATAAA 
GGACAGACTG TTCCAGTAGA TGACCACTAC TGCAGTGACC AACTTAAACC TCCAACCCGA 
GAACCCTGCC ATGGTGCCTG TGTCTTAACA AGGTGGCATT ATTTAGAATG GTCTCAGTGT 
TCCAGGAGTT GCGGAGGAGG GGAGAGGTCT CGAGAATCTT ACTGTATAAA TAACTTTGGC 
CACCGTCTTG CTGACAGAGA ATGCCAGGAA CTTCCCCGAG TGACAACAGA GAATTGCAAT 
GAATTTTCCT GTCCCAGTTG GGCCACTAGT GAGTGGAGTG AGTGTCTGGT TACCTGTGGA 
AAAGGAACAA AGCAGCGGCA GGTGTGGTGT CACCTGAATG AAGATCACTT GAGTGATGGC 
TTCTGTGACC CAAGCACCAA GCCTGAGTCT CAGAGACCCT GTGAGCTTCA TGCATGTACT 
TCCTGGCAAG TAGGACCTTG GGGTTCTTGC ACAGCCACGT GTGGACAGGG GTACCAGATG 
CGTGCTGTGA AGTGTGTCAA CGAGCTATTT AGCACAGTGC TAGATGACAG ACTGTGCCAT 
GGAGCTAGTC GCCCAAGTGA AAGGCAGAAT TGTATTGTTA CACCTTGCCC AGTTATCCCT 
AAAATTGGGG CCACTTCCTT GCCAGCTGTT CCCATGAGAA AGATAACACA ATGGCGATAT 
GGTTCTTGGA CACCATGCTC CGTGTCTTGT GGAAGAGGAA ATCAAGCCCG CTATGTAAGT 
TGTCGTGATG CTCATGATGG AATAGCAGAT GAATCATTTT GTGCCCACTT ACCCCGACCG 
GCTGAAATAG CTGTCTGTTT TAGCCCTTGT GGAGAGTGGC AAACAGGGAA TTGGTCACCT 
TGTTCAGCTT CCTGTGGCCG TGGAAAAATG ACTCGACAAG TTTTATGCAT CAACTATCAT 
CAGCCAGTTA ATGAGAATTA CTGTGACCCT GAAGTCCGCC CTGTGATAGA ACAAGAATGT 
AATCTGGCAG CCTGCCCTCC CACGTACAGC CACTTTCCGA GTTCCTCTGA GCAGCCAAGC 
CATTTTCCAG GCAGGAATTT TCCATTAACT CACAAACCAG AAGATAATCA AAATCAGAGG 
GTCCATCCAT CAATCAGAGA AAACCAGTGG AGAACAGGAC CATGGGGATC ATGCTCTAGC 
AGTTGTGCTG GAGGTGTTCA GCGTAGAGTT GTGGTCTGCC AGGATGAAAA TGGACAAAGT 
GCCAGTTACT GTGATGCAGC GTCCAAACCC CCAGAGTCAA AGCACTGTGA TTCAGGACCT 
TGTCCACAGT GGAACTATGG AAGCTGGGGA GAATGTACGC AAACGTGTGG AGGAGGAATA 
AAATCAAGAT TTGTAATATG CCAGTTTCCC GATGGCCAGC TGTCACAAGA GCAAAACTGT 
GAAATGTTGA ACAAGCCGCC TAGTGTGGTA CAGTGTCATA TACATGCTTG CCCTGATGAC 
GTATCATGGC ATCGGGGACC ATGGAAATCG TGCTCAGCTT CCTGTGGTAA AGGTTTAAAA 
TACCGTGAGG TGCTTTGCAT TGACCAATTC CACAGGAAAT TAGAAGAAAA ATATTGCAGT 
CATCTACAGA AACCTCGAAC ACATAAAGCT TGTAGATCTG TCCGATGCCC TTCATGGAAA 
GCCAACAGAT GGAAGGAGTG CTCTGTGACC TGCGGCCCCG GAGTTCAGCG GAGGGATGTG 
TACTGCAGAC TCAGAGGAGT TGGTCCGGTG GCGGAAGAAA AGTGTGATCC ATCCACGCGG 
CCTTATTTTC AGAGAGAGTG TTGGCATCGG GACTGCATAC AGTACCACTG GGCGGCAGGA 
GAGTGGTTGG ATTGCTCAGT ATCGTGTAAG AAAAGAGAGA CATATCGGCA AGTGAAGTGC 
ATGGATGCAC AAAACATTCA AGTGAGTGAG AGTTTCTGTG ACCTCGCGAC CAGACCTCTC 
GCGGTGAAAA AGTGCAGAAA CTCTCCCTGC AAGTATATCG TGGTAACAGG AGACTCATCC 
CAGTGTGCAG GTAACTGTGG ATTTAGTTAT CGACAAAGGA TTACATATTG CGTTGGGGTC 
CAATCTATGG AGAAACATAA ACTTCATGAG CTGTGGCCTA TAGACTATCG AGTATGCCCT 
GTGCTGCCTT CCCCTCAGGT TTACAAATGC AATTTTAGGA CCTGTTTGCA TATGGCCACT 
TGGAAAGTTG GAAAATGGAG CAAGTGCTCT GTGACTTGTG GAGTTGGGAT GATGGAGAGA 
AGAGTGGAAT GCATGGCCGA TAATGGTCTG TCCAGTGATT TATGTTTAAA GCATTTAAAA 
CCAGATGCTC AAAAGAAATG TTATGTCAGT GACTGTAAAA CATTTATGAG CTGTAAAGAA 
ATACAAGTGA AAAACAACAT TACCAAGGAT GGTGACTATT ACCTTAACAT TAAAGGAAGA 
ATAACAAAGA TCTATTGTGC TGGCATGCAC TTGGAGAACC CTAAGGAATA TATATCATTG 
GTCAAAGGTG AAGAAGACAA CTTTTCTGAA GTGTATGGTT TTAGACTACG AAATCCATAT 
GAATGTCCTT TTAATGGAAG CAGGAGGCAT GACTGTGAAT GTAAAAATGA CTATCTGGCT 
GCTGGACACA CTGTTTTTAG CAAAATAAGA ATTGATCTTA TTTCCATGCA AATTAAAACT 
ACAGACCTTC TTTTTGCCCA GACAATATTT GGAAATGCAG TTCCATTTGC CACAGCTGGA 
GATTGCTATA GTGCTGCCAG ATGCCCACAG GGACAGTTCA GCATTAATTT GGCAGGAACT 
GGGATGAAGA TATCCAGTAC AGCAAAGTGG CTGGCTCAGG GCAGTTATGC CTCTGTGATC 
ATTCACAGAT CACAGGATGG AACCAAAGTC TATGGCAGAT GTGGAGGGTT CTGTGGAAAG 
TGTATTCCTC ACATGACTAC TGGTCTCCCA ATTCAAGTAG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ADAMTS20 ( XM_004006435.3 ) cDNA ORF clone, Ovis aries musimon(Ovis orientalis musimon) -> XP_004006484.1 Ovis aries ADAM metallopeptidase with thrombospondin type 1 motif 20 (ADAMTS20), transcript variant X1, mRNA.

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CloneID	OSm04356
Clone ID Related Accession (Same CDS sequence)	XM_004006435.3
Accession Version	XM_004006435.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5748bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries(sheep)
Product	A disintegrin and metalloproteinase with thrombospondin motifs 20 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014639012.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_004006435.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGGTGG TCAAGTGGCT GACGGGGCTG CTCTACCAGC TCTCGCTCTT CATCACCAGG 
TCTTGGGAAG TTCACTTCCA CCCCAGGCAG GAAGCCTTAG TGAGGACACT GGCCTCCTAC 
GAAGTGGTGA CCCCCGCGCG GGTCAATGAG TTCGGCGAAG TATTCCCGCA GAGCCACCAC 
TTCAGCCGGA GGAAGCGCAG CTCGGAGACT CTGGAGCCCA CACCGTTCAG AACCCACTAC 
CGAATCCGCG CCTACGGGCA GCTCTTCCAA CTGAACCTGA GCGCCGACGC GGCCTTCCTG 
GCTGCGGGAT ACACCGAGGT GCACTTAGGC ACCTCTGCGC ACCCGGCGGA GGAGCGCAGC 
GCGGCGCCCG CAGACCTGCG CCACTGCTTC TACCGCGGCC AGGTCAATGC TCGCGAGGAT 
CACACAGCCG TCTTCAGCCT CTGTGGAGGC TTGATGGGAA CATTTAAAGC ACATGATGGT 
GAATATTTCT TAGAACCTAT AATGAAGGCA GATGGGAGTG AACATGAAGA TGATCATAGC 
AAGCCACATC TTATATACAG ACAGGAATTA AAGGGGAACT ATTTTCTGCA GTCTCACAAG 
CCATGTGATG CTTCAGAAAG TGAAATAAAG AAAACTACTC TACCCTTTCA TAACTACAGC 
GACATTAATG AAAATCTCAA TATAAAGGAA GAAAGAGTTT TAGGATACAC TTCAAAAAAT 
ATGTCTTTGG AAGATGAAAG AAGTCAATTG CATTCCAGGA AGAAACGTTT TTTATCATAT 
CCAAGATACG TGGAAGTTAT GGTTACAGCT GACTCTAAAA TGGTTCATCA TCATGGACAG 
AACTTGCAGC ACTACGTACT AACTCTGATG TCAATTGTTG CAGCAATCTA CAAGGACTCA 
AGTATTGGAA ATCTTATAAA CATAGTAATT GTAAAATTAG TTATAATTCA TGATGAGCAG 
GAAGGACCAG TCATCAGTTT TAATGCAGCT ACCACGTTAC GCAACTTCTG TTTGTGGCAA 
CAAACTCAGA ATGTTCTTGA CGATGCCCAC CCATCCCACC ATGATACTGC TGTTCTTATC 
ACAAGGGAAG ACATTTGTGG AGCTAGAGAG AAATGTGACA CATTAGGTTT AGCAGAATTA 
GGTACCCTGT GTGACCCTTT ACGGAGCTGC TCTATTAGTG AAGAAAATGG GCTCAGTGCT 
GCCTTTACCA TAGCCCATGA GCTTGGTCAT GTATTTAATG TTCCACACGA TGATAGTTTT 
AAATGTAAGG AAACTGGAAT TAAACATCAG TATCATGTCA TGGCTCCAAC TTTAAATTAC 
CACACAAGTC CTTGGACCTG GTCAAAATGT AGTCAGAAAT ATATCACTGA ATTCCTAGAC 
ACCGGTCATG GGGAATGCCT CCTTGACAAA CCAAATGGAA GAATATACGA TTTGTCTTCA 
CAGCTTCCTG GATTAATGTA TGATGTAAAC AAGCAATGTG AGCTTATGTT TGGTCCTGGA 
TCACAAGTGT GTCCCTATTT AAAACAATGC AGGCGTCTCT GGTGTACCAG CGCAGAAGGA 
GTCCACAAGG GCTGTCGCAC TCAACACATG CCTCTGGCAG ACGGAACAAA CTGTGGTCCT 
GGGATGCATT GCCACCATGG GCTATGTGTA AATAAAGAAA TGGAGACACG TCCTGTAGAT 
GGAGATTGGG GACCATGGGG ACCTTACAGT TCTTGTTCAA GAACATGTGG AGGTGGAATC 
AAAAGCACAA GCAGGCTCTG TAATCGCCCC GAGCCAAGAA ACGGAGGCAA GTACTGTGTG 
GGCCGCAGGA TGAAATTTCG ATCATGTAAC ACTGATTCAT GTCCAAAAGG CAAGCAAGAC 
TTCCGTGAGA AGCAGTGCTC TGATTTTGAT GGTAAACATT TCAACATCAA TGGTCTCTCC 
CCTAACGTGA GGTGGCTTCC AAAGTACAGT GGGATTGCTG TAAAAGATCG TTGTAAACTC 
TATTGTCGGG TGGCTGGAAC CACTAGCTTC TACCAACTGA AGGACAGGGT TGCCGATGGT 
ACTCCTTGTG GAACGGAAAC AAATGACATC TGTGTTCAAG GCCTGTGTCG GCAAGCTGGC 
TGTGATCACG TGTTAAACTC CAAGGCCAGG AGAGACAAAT GTGGCGTGTG TGGTGGGGAT 
AACTCCTCCT GCAGGACAAT GGCAGGCGTC TTCAACAGTG CGCATTATGG TTATAATGTC 
GTTGTAAAGA TTCCCCCAGG AGCAACAAAC ATTGATATTC TTCAGCACAG CTATTCTGGA 
AAACCAGAAG ATGACAATTA CCTGGCATTA TCGGACACTC AAGGGAATTT TCTTTTGAAT 
GGGAATTTTG TTGTAAGTAT GTCCAAACGG GAAATCAACG TACAAGGAGC TATTTTTGAA 
TACAGTGGTT CAAACAACTC AATTGAAAGG ATTAATAGTA CTGATCGACT GGAAGAAGAA 
CTTGTTTTGC AGGTTTTGTG TGTGGGTAAT CTATACAACC CTGATGTACG TTATTCCTTC 
AATATCCCCG TGGAAGAGAA GAGTGATCTA TTCACATGGG ACCCGTATGG ACCATGGCAA 
GACTGTACAA AAATGTGTCA AGGTCTTCAT AGAAGAAAAA TTACTTGTGT ACGTAGGAGT 
GATCATATGG TCATGTCTGA TCAAAGCTGT GACCACTTAC CACTTCCATT GCTGGTAACT 
GAAAGGTGCA ATACAGACTG TGAACTCAGG TGGCACATCA TTGGCAAAAG TGAATGTTCA 
ACCCTTTGTG GTCAAGGATA CAAGTCATTG GACGTCCATT GTATGAAGTA TTCCATTCAT 
AAAGGACAGA CTGTTCCAGT AGATGACCAC TACTGCAGTG ACCAACTTAA ACCTCCAACC 
CGAGAACCCT GCCATGGTGA CTGTGTCTTA ACAAGGTGGC ATTATTTAGA ATGGTCTCAG 
TGTTCCAGGA GTTGCGGAGG AGGGGAGAGG TCTCGAGAAT CTTACTGTAT AAATAACTTT 
GGCCACCGTC TTGCTGACAG AGAATGCCAG GAACTTCCCC GAGTGACAAC AGAGAATTGC 
AATGAATTTT CCTGTCCCAG TTGGGCCACT AGTGAGTGGA GTGAGTGTCT GGTTACCTGT 
GGAAAAGGAA CAAAGCAGCG GCAGGTGTGG TGTCACCTGA ATGAAGATCA CTTGAGTGAT 
GGCTTCTGTG ACCCAAGCAC CAAGCCTGAG TCTCAGAGAC CCTGTGAGCT TCATGCATGT 
ACTTCCTGGC AAGTAGGACC TTGGGGTTCT TGCACAGCCA CGTGTGGACA GGGGTACCAG 
ATGCGTGCTG TGAAGTGTGT CAACGAGCTA TTTAGCACAG TGCTAGATGA CAGACTGTGC 
CATGGAGCTA GTCGCCCAAG TGAAAGGCAG AATTGTATTG TTACACCTTG CCCAGTTATC 
CCTAAAATTG GGGCCACTTC CTTGCCAGCT GTTCCCATGA GAAAGATAAC ACAATGGCGA 
TATGGTTCTT GGACACCATG CTCCGTGTCT TGTGGAAGAG GAAATCAAGC CCGCTATGTA 
AGTTGTCGTG ATGCTCATGA TGGAATAGCA GATGAATCAT TTTGTGCCCA CTTACCCCGA 
CCGGCTGAAA TAGCTGTCTG TTTTAGCCCT TGTGGAGAGT GGCAAACAGG GAATTGGTCA 
CCTTGTTCAG CTTCCTGTGG CCGTGGAAAA ATGACTCGAC AAGTTTTATG CATCAACTAT 
CATCAGCCAG TTAATGAGAA TTACTGTGAC CCTGAAGTCC GCCCTGTGAT AGAACAAGAA 
TGTAATCTGG CAGCCTGCCC TCCCACGTAC AGCCACTTTC CGAGTTCCTC TGAGCAGCCA 
AGCCATTTTC CAGGCAGGAA TTTTCCATTA ACTCACAAAC CAGAAGATAA TCAAAATCAG 
AGGGTCCATC CATCAATCAG AGAAAACCAG TGGAGAACAG GACCATGGGG ATCATGCTCT 
AGCAGTTGTG CTGGAGGTGT TCAGCGTAGA GTTGTGGTCT GCCAGGATGA AAATGGACAA 
AGTGCCAGTT ACTGTGATGC AGCGTCCAAA CCCCCAGAGT CAAAGCACTG TGATTCAGGA 
CCTTGTCCAC AGTGGAACTA TGGAAGCTGG GGAGAATGTA CGCAAACGTG TGGAGGAGGA 
ATAAAATCAA GATTTGTAAT ATGCCAGTTT CCCGATGGCC AGCTGTCACA AGAGCAAAAC 
TGTGAAATGT TGAACAAGCC GCCTAGTGTG GTACAGTGTC ATATACATGC TTGCCCTGAT 
GACGTATCAT GGCATCGGGG ACCATGGAAA TCGTGCTCAG CTTCCTGTGG TAAAGGTTTA 
AAATACCGTG AGGTGCTTTG CATTGACCAA TTCCACAGGA AATTAGAAGA AAAATATTGC 
AGTCATCTAC AGAAACCTCG AACACATAAA GCTTGTAGAT CTGTCCGATG CCCTTCATGG 
AAAGCCAACA GATGGAAGGA GTGCTCTGTG ACCTGCGGCT CCGGAGTTCA GCAGAGGGAT 
GTGTACTGCA GACTCAGAGG AGTTGGTCCG GTGGCGGAAG AAAAGTGTGA TCCATCCACG 
CGGCCTTATT TTCAGAGAGA GTGTTGGCAT CGGGACTGCA TACAGCACCA CTGGGCGGCA 
GGAGAGTGGT TGGATTGCTC AGTATCGTGT AAGAAAAGAG AGACATATCG GCAAGTGAAG 
TGCATGGATG CACAAAACAT TCAAGTGAGT GAGAGTTTCT GTGACCTCGC GACCAGACCT 
CTCGCGGTGA AAAAGTGCAG AAACTCTCCC TGCAAGTATA TCGTGGTAAC AGGAGACTCA 
TCCCAGTGTG CAGGTAACTG TGGATTTAGT TATCGACAAA GGATTACATA TTGCGTTGGG 
GTCCAATCTA TGGAGAAACA TAAACTTCAT GAGCTATGGC CTATAGACTA TCGAGTATGC 
CCTGTGCTGC CTTCCCCTCA GGTTTACAAA TGCAATTTTA GGACCTGTTT GCATATGGCC 
ACTTGGAAAG TTGGAAAATG GAGCAAGTGC TCTGTGACTT GTGGAGTTGG GATGATGGAG 
AGAAGAGTGG AATGCATGGC TGATAATGGT CTGTCCAGTG ATTTATGTTT AAAGCATTTA 
AAACCAGATG CTCAAAAGAA ATGTTATGTC AGTGACTGTA AAACATTTAT GAGCTGTAAA 
GAAATACAAG TGAAAAACAA CATTACCAAG GATGGTGACT ATTACCTTAA CATTAAAGGA 
AGAATAACAA AGATCTATTG TGCTGGCATG CACTTGGAGA ACCCTAAGGA ATATTTATCA 
TTGGTCAAAG GTGAAGAAGA CAACTTTTCT GAAGTGTATG GTTTTAGACT ACGAAATCCA 
TATGAATGTC CTTTTAATGG AAGCAGGAGG CATGACTGTG AATGTAAAAA TGACTATCTG 
GCTGCTGGAC ACACTGTTTT TAGCAAAATA AGAATTGATC TTACTTCCAT GCAAATTAAA 
ACTACAGACC TTCTTTTTGC CCAGACAATA TTTGGAAATG CAGTTCCATT TGCCACAGCT 
GGAGATTGCT ATAGTGCTGC CAGATGCCCA CAGGGACAGT TCAGCATTAA TTTGGCAGGA 
ACTGGGATGA AGATATCCAG TACAGCAAAG TGGCTGGCTC AGGGCAGTTA TGCCTCTGTG 
ATCATTCACA GATCACAGGA TGGAACCAAA GTCTATGGCA GATGTGGAGG GTTCTGTGGA 
AAGTGTATTC CTCACATGAC TACTGGTCTC CCAATTCAAG TAGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004006484.1
CDS	47..5794
Translation	MRVVKWLTGLLYQLSLFITRSWEVHFHPRQEALVRTLASYEVVTPARVNEFGEVFPQSHHFSRRKRSSETLEPTPFRTHYRIRAYGQLFQLNLSADAAFLAAGYTEVHLGTSAHPAEERSAAPADLRHCFYRGQVNAREDHTAVFSLCGGLMGTFKAHDGEYFLEPIMKADGSEHEDDHSKPHLIYRQELKGNYFLQSHKPCDASESEIKKTTLPFHNYSDINENLNIKEERVLGYTSKNMSLEDERSQLHSRKKRFLSYPRYVEVMVTADSKMVHHHGQNLQHYVLTLMSIVAAIYKDSSIGNLINIVIVKLVIIHDEQEGPVISFNAATTLRNFCLWQQTQNVLDDAHPSHHDTAVLITREDICGAREKCDTLGLAELGTLCDPLRSCSISEENGLSAAFTIAHELGHVFNVPHDDSFKCKETGIKHQYHVMAPTLNYHTSPWTWSKCSQKYITEFLDTGHGECLLDKPNGRIYDLSSQLPGLMYDVNKQCELMFGPGSQVCPYLKQCRRLWCTSAEGVHKGCRTQHMPLADGTNCGPGMHCHHGLCVNKEMETRPVDGDWGPWGPYSSCSRTCGGGIKSTSRLCNRPEPRNGGKYCVGRRMKFRSCNTDSCPKGKQDFREKQCSDFDGKHFNINGLSPNVRWLPKYSGIAVKDRCKLYCRVAGTTSFYQLKDRVADGTPCGTETNDICVQGLCRQAGCDHVLNSKARRDKCGVCGGDNSSCRTMAGVFNSAHYGYNVVVKIPPGATNIDILQHSYSGKPEDDNYLALSDTQGNFLLNGNFVVSMSKREINVQGAIFEYSGSNNSIERINSTDRLEEELVLQVLCVGNLYNPDVRYSFNIPVEEKSDLFTWDPYGPWQDCTKMCQGLHRRKITCVRRSDHMVMSDQSCDHLPLPLLVTERCNTDCELRWHIIGKSECSTLCGQGYKSLDVHCMKYSIHKGQTVPVDDHYCSDQLKPPTREPCHGDCVLTRWHYLEWSQCSRSCGGGERSRESYCINNFGHRLADRECQELPRVTTENCNEFSCPSWATSEWSECLVTCGKGTKQRQVWCHLNEDHLSDGFCDPSTKPESQRPCELHACTSWQVGPWGSCTATCGQGYQMRAVKCVNELFSTVLDDRLCHGASRPSERQNCIVTPCPVIPKIGATSLPAVPMRKITQWRYGSWTPCSVSCGRGNQARYVSCRDAHDGIADESFCAHLPRPAEIAVCFSPCGEWQTGNWSPCSASCGRGKMTRQVLCINYHQPVNENYCDPEVRPVIEQECNLAACPPTYSHFPSSSEQPSHFPGRNFPLTHKPEDNQNQRVHPSIRENQWRTGPWGSCSSSCAGGVQRRVVVCQDENGQSASYCDAASKPPESKHCDSGPCPQWNYGSWGECTQTCGGGIKSRFVICQFPDGQLSQEQNCEMLNKPPSVVQCHIHACPDDVSWHRGPWKSCSASCGKGLKYREVLCIDQFHRKLEEKYCSHLQKPRTHKACRSVRCPSWKANRWKECSVTCGSGVQQRDVYCRLRGVGPVAEEKCDPSTRPYFQRECWHRDCIQHHWAAGEWLDCSVSCKKRETYRQVKCMDAQNIQVSESFCDLATRPLAVKKCRNSPCKYIVVTGDSSQCAGNCGFSYRQRITYCVGVQSMEKHKLHELWPIDYRVCPVLPSPQVYKCNFRTCLHMATWKVGKWSKCSVTCGVGMMERRVECMADNGLSSDLCLKHLKPDAQKKCYVSDCKTFMSCKEIQVKNNITKDGDYYLNIKGRITKIYCAGMHLENPKEYLSLVKGEEDNFSEVYGFRLRNPYECPFNGSRRHDCECKNDYLAAGHTVFSKIRIDLTSMQIKTTDLLFAQTIFGNAVPFATAGDCYSAARCPQGQFSINLAGTGMKISSTAKWLAQGSYASVIIHRSQDGTKVYGRCGGFCGKCIPHMTTGLPIQVV

Target ORF information:

RefSeq Version	XM_004006435.3
Organism	Ovis aries(sheep)
Definition	Ovis aries ADAM metallopeptidase with thrombospondin type 1 motif 20 (ADAMTS20), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004006435.3

ORF Insert Sequence:

ATGCGGGTGG TCAAGTGGCT GACGGGGCTG CTCTACCAGC TCTCGCTCTT CATCACCAGG 
TCTTGGGAAG TTCACTTCCA CCCCAGGCAG GAAGCCTTAG TGAGGACACT GGCCTCCTAC 
GAAGTGGTGA CCCCCGCGCG GGTCAATGAG TTCGGCGAAG TATTCCCGCA GAGCCACCAC 
TTCAGCCGGA GGAAGCGCAG CTCGGAGACT CTGGAGCCCA CACCGTTCAG AACCCACTAC 
CGAATCCGCG CCTACGGGCA GCTCTTCCAA CTGAACCTGA GCGCCGACGC GGCCTTCCTG 
GCTGCGGGAT ACACCGAGGT GCACTTAGGC ACCTCTGCGC ACCCGGCGGA GGAGCGCAGC 
GCGGCGCCCG CAGACCTGCG CCACTGCTTC TACCGCGGCC AGGTCAATGC TCGCGAGGAT 
CACACAGCCG TCTTCAGCCT CTGTGGAGGC TTGATGGGAA CATTTAAAGC ACATGATGGT 
GAATATTTCT TAGAACCTAT AATGAAGGCA GATGGGAGTG AACATGAAGA TGATCATAGC 
AAGCCACATC TTATATACAG ACAGGAATTA AAGGGGAACT ATTTTCTGCA GTCTCACAAG 
CCATGTGATG CTTCAGAAAG TGAAATAAAG AAAACTACTC TACCCTTTCA TAACTACAGC 
GACATTAATG AAAATCTCAA TATAAAGGAA GAAAGAGTTT TAGGATACAC TTCAAAAAAT 
ATGTCTTTGG AAGATGAAAG AAGTCAATTG CATTCCAGGA AGAAACGTTT TTTATCATAT 
CCAAGATACG TGGAAGTTAT GGTTACAGCT GACTCTAAAA TGGTTCATCA TCATGGACAG 
AACTTGCAGC ACTACGTACT AACTCTGATG TCAATTGTTG CAGCAATCTA CAAGGACTCA 
AGTATTGGAA ATCTTATAAA CATAGTAATT GTAAAATTAG TTATAATTCA TGATGAGCAG 
GAAGGACCAG TCATCAGTTT TAATGCAGCT ACCACGTTAC GCAACTTCTG TTTGTGGCAA 
CAAACTCAGA ATGTTCTTGA CGATGCCCAC CCATCCCACC ATGATACTGC TGTTCTTATC 
ACAAGGGAAG ACATTTGTGG AGCTAGAGAG AAATGTGACA CATTAGGTTT AGCAGAATTA 
GGTACCCTGT GTGACCCTTT ACGGAGCTGC TCTATTAGTG AAGAAAATGG GCTCAGTGCT 
GCCTTTACCA TAGCCCATGA GCTTGGTCAT GTATTTAATG TTCCACACGA TGATAGTTTT 
AAATGTAAGG AAACTGGAAT TAAACATCAG TATCATGTCA TGGCTCCAAC TTTAAATTAC 
CACACAAGTC CTTGGACCTG GTCAAAATGT AGTCAGAAAT ATATCACTGA ATTCCTAGAC 
ACCGGTCATG GGGAATGCCT CCTTGACAAA CCAAATGGAA GAATATACGA TTTGTCTTCA 
CAGCTTCCTG GATTAATGTA TGATGTAAAC AAGCAATGTG AGCTTATGTT TGGTCCTGGA 
TCACAAGTGT GTCCCTATTT AAAACAATGC AGGCGTCTCT GGTGTACCAG CGCAGAAGGA 
GTCCACAAGG GCTGTCGCAC TCAACACATG CCTCTGGCAG ACGGAACAAA CTGTGGTCCT 
GGGATGCATT GCCACCATGG GCTATGTGTA AATAAAGAAA TGGAGACACG TCCTGTAGAT 
GGAGATTGGG GACCATGGGG ACCTTACAGT TCTTGTTCAA GAACATGTGG AGGTGGAATC 
AAAAGCACAA GCAGGCTCTG TAATCGCCCC GAGCCAAGAA ACGGAGGCAA GTACTGTGTG 
GGCCGCAGGA TGAAATTTCG ATCATGTAAC ACTGATTCAT GTCCAAAAGG CAAGCAAGAC 
TTCCGTGAGA AGCAGTGCTC TGATTTTGAT GGTAAACATT TCAACATCAA TGGTCTCTCC 
CCTAACGTGA GGTGGCTTCC AAAGTACAGT GGGATTGCTG TAAAAGATCG TTGTAAACTC 
TATTGTCGGG TGGCTGGAAC CACTAGCTTC TACCAACTGA AGGACAGGGT TGCCGATGGT 
ACTCCTTGTG GAACGGAAAC AAATGACATC TGTGTTCAAG GCCTGTGTCG GCAAGCTGGC 
TGTGATCACG TGTTAAACTC CAAGGCCAGG AGAGACAAAT GTGGCGTGTG TGGTGGGGAT 
AACTCCTCCT GCAGGACAAT GGCAGGCGTC TTCAACAGTG CGCATTATGG TTATAATGTC 
GTTGTAAAGA TTCCCCCAGG AGCAACAAAC ATTGATATTC TTCAGCACAG CTATTCTGGA 
AAACCAGAAG ATGACAATTA CCTGGCATTA TCGGACACTC AAGGGAATTT TCTTTTGAAT 
GGGAATTTTG TTGTAAGTAT GTCCAAACGG GAAATCAACG TACAAGGAGC TATTTTTGAA 
TACAGTGGTT CAAACAACTC AATTGAAAGG ATTAATAGTA CTGATCGACT GGAAGAAGAA 
CTTGTTTTGC AGGTTTTGTG TGTGGGTAAT CTATACAACC CTGATGTACG TTATTCCTTC 
AATATCCCCG TGGAAGAGAA GAGTGATCTA TTCACATGGG ACCCGTATGG ACCATGGCAA 
GACTGTACAA AAATGTGTCA AGGTCTTCAT AGAAGAAAAA TTACTTGTGT ACGTAGGAGT 
GATCATATGG TCATGTCTGA TCAAAGCTGT GACCACTTAC CACTTCCATT GCTGGTAACT 
GAAAGGTGCA ATACAGACTG TGAACTCAGG TGGCACATCA TTGGCAAAAG TGAATGTTCA 
ACCCTTTGTG GTCAAGGATA CAAGTCATTG GACGTCCATT GTATGAAGTA TTCCATTCAT 
AAAGGACAGA CTGTTCCAGT AGATGACCAC TACTGCAGTG ACCAACTTAA ACCTCCAACC 
CGAGAACCCT GCCATGGTGA CTGTGTCTTA ACAAGGTGGC ATTATTTAGA ATGGTCTCAG 
TGTTCCAGGA GTTGCGGAGG AGGGGAGAGG TCTCGAGAAT CTTACTGTAT AAATAACTTT 
GGCCACCGTC TTGCTGACAG AGAATGCCAG GAACTTCCCC GAGTGACAAC AGAGAATTGC 
AATGAATTTT CCTGTCCCAG TTGGGCCACT AGTGAGTGGA GTGAGTGTCT GGTTACCTGT 
GGAAAAGGAA CAAAGCAGCG GCAGGTGTGG TGTCACCTGA ATGAAGATCA CTTGAGTGAT 
GGCTTCTGTG ACCCAAGCAC CAAGCCTGAG TCTCAGAGAC CCTGTGAGCT TCATGCATGT 
ACTTCCTGGC AAGTAGGACC TTGGGGTTCT TGCACAGCCA CGTGTGGACA GGGGTACCAG 
ATGCGTGCTG TGAAGTGTGT CAACGAGCTA TTTAGCACAG TGCTAGATGA CAGACTGTGC 
CATGGAGCTA GTCGCCCAAG TGAAAGGCAG AATTGTATTG TTACACCTTG CCCAGTTATC 
CCTAAAATTG GGGCCACTTC CTTGCCAGCT GTTCCCATGA GAAAGATAAC ACAATGGCGA 
TATGGTTCTT GGACACCATG CTCCGTGTCT TGTGGAAGAG GAAATCAAGC CCGCTATGTA 
AGTTGTCGTG ATGCTCATGA TGGAATAGCA GATGAATCAT TTTGTGCCCA CTTACCCCGA 
CCGGCTGAAA TAGCTGTCTG TTTTAGCCCT TGTGGAGAGT GGCAAACAGG GAATTGGTCA 
CCTTGTTCAG CTTCCTGTGG CCGTGGAAAA ATGACTCGAC AAGTTTTATG CATCAACTAT 
CATCAGCCAG TTAATGAGAA TTACTGTGAC CCTGAAGTCC GCCCTGTGAT AGAACAAGAA 
TGTAATCTGG CAGCCTGCCC TCCCACGTAC AGCCACTTTC CGAGTTCCTC TGAGCAGCCA 
AGCCATTTTC CAGGCAGGAA TTTTCCATTA ACTCACAAAC CAGAAGATAA TCAAAATCAG 
AGGGTCCATC CATCAATCAG AGAAAACCAG TGGAGAACAG GACCATGGGG ATCATGCTCT 
AGCAGTTGTG CTGGAGGTGT TCAGCGTAGA GTTGTGGTCT GCCAGGATGA AAATGGACAA 
AGTGCCAGTT ACTGTGATGC AGCGTCCAAA CCCCCAGAGT CAAAGCACTG TGATTCAGGA 
CCTTGTCCAC AGTGGAACTA TGGAAGCTGG GGAGAATGTA CGCAAACGTG TGGAGGAGGA 
ATAAAATCAA GATTTGTAAT ATGCCAGTTT CCCGATGGCC AGCTGTCACA AGAGCAAAAC 
TGTGAAATGT TGAACAAGCC GCCTAGTGTG GTACAGTGTC ATATACATGC TTGCCCTGAT 
GACGTATCAT GGCATCGGGG ACCATGGAAA TCGTGCTCAG CTTCCTGTGG TAAAGGTTTA 
AAATACCGTG AGGTGCTTTG CATTGACCAA TTCCACAGGA AATTAGAAGA AAAATATTGC 
AGTCATCTAC AGAAACCTCG AACACATAAA GCTTGTAGAT CTGTCCGATG CCCTTCATGG 
AAAGCCAACA GATGGAAGGA GTGCTCTGTG ACCTGCGGCT CCGGAGTTCA GCAGAGGGAT 
GTGTACTGCA GACTCAGAGG AGTTGGTCCG GTGGCGGAAG AAAAGTGTGA TCCATCCACG 
CGGCCTTATT TTCAGAGAGA GTGTTGGCAT CGGGACTGCA TACAGCACCA CTGGGCGGCA 
GGAGAGTGGT TGGATTGCTC AGTATCGTGT AAGAAAAGAG AGACATATCG GCAAGTGAAG 
TGCATGGATG CACAAAACAT TCAAGTGAGT GAGAGTTTCT GTGACCTCGC GACCAGACCT 
CTCGCGGTGA AAAAGTGCAG AAACTCTCCC TGCAAGTATA TCGTGGTAAC AGGAGACTCA 
TCCCAGTGTG CAGGTAACTG TGGATTTAGT TATCGACAAA GGATTACATA TTGCGTTGGG 
GTCCAATCTA TGGAGAAACA TAAACTTCAT GAGCTATGGC CTATAGACTA TCGAGTATGC 
CCTGTGCTGC CTTCCCCTCA GGTTTACAAA TGCAATTTTA GGACCTGTTT GCATATGGCC 
ACTTGGAAAG TTGGAAAATG GAGCAAGTGC TCTGTGACTT GTGGAGTTGG GATGATGGAG 
AGAAGAGTGG AATGCATGGC TGATAATGGT CTGTCCAGTG ATTTATGTTT AAAGCATTTA 
AAACCAGATG CTCAAAAGAA ATGTTATGTC AGTGACTGTA AAACATTTAT GAGCTGTAAA 
GAAATACAAG TGAAAAACAA CATTACCAAG GATGGTGACT ATTACCTTAA CATTAAAGGA 
AGAATAACAA AGATCTATTG TGCTGGCATG CACTTGGAGA ACCCTAAGGA ATATTTATCA 
TTGGTCAAAG GTGAAGAAGA CAACTTTTCT GAAGTGTATG GTTTTAGACT ACGAAATCCA 
TATGAATGTC CTTTTAATGG AAGCAGGAGG CATGACTGTG AATGTAAAAA TGACTATCTG 
GCTGCTGGAC ACACTGTTTT TAGCAAAATA AGAATTGATC TTACTTCCAT GCAAATTAAA 
ACTACAGACC TTCTTTTTGC CCAGACAATA TTTGGAAATG CAGTTCCATT TGCCACAGCT 
GGAGATTGCT ATAGTGCTGC CAGATGCCCA CAGGGACAGT TCAGCATTAA TTTGGCAGGA 
ACTGGGATGA AGATATCCAG TACAGCAAAG TGGCTGGCTC AGGGCAGTTA TGCCTCTGTG 
ATCATTCACA GATCACAGGA TGGAACCAAA GTCTATGGCA GATGTGGAGG GTTCTGTGGA 
AAGTGTATTC CTCACATGAC TACTGGTCTC CCAATTCAAG TAGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ADAMTS20 ( XM_012174462.2 ) cDNA ORF clone, Ovis aries musimon(Ovis orientalis musimon) -> XP_012029852.1 Ovis aries ADAM metallopeptidase with thrombospondin type 1 motif 20 (ADAMTS20), transcript variant X2, mRNA.

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CloneID	OSm36428
Clone ID Related Accession (Same CDS sequence)	XM_012174462.2 , XM_015094726.1
Accession Version	XM_012174462.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4665bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries(sheep)
Product	A disintegrin and metalloproteinase with thrombospondin motifs 20 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014639012.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012174462.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTTGG TCATGTGTCA GACAGGCTAC ACAGATCAAC AGACTAAGCA AAGAACTAGA 
AATTTGGAAC TCTTTTCTTT CATTTACTGT ATTGGATTAT ATGACAAGCA CTTCTGGCCT 
CTATCCCTTG TTATCCTATG CATGACCAGA TTTAATGTTC CACACGATGA TAGTTTTAAA 
TGTAAGGAAA CTGGAATTAA ACATCAGTAT CATGTCATGG CTCCAACTTT AAATTACCAC 
ACAAGTCCTT GGACCTGGTC AAAATGTAGT CAGAAATATA TCACTGAATT CCTAGACACC 
GGTCATGGGG AATGCCTCCT TGACAAACCA AATGGAAGAA TATACGATTT GTCTTCACAG 
CTTCCTGGAT TAATGTATGA TGTAAACAAG CAATGTGAGC TTATGTTTGG TCCTGGATCA 
CAAGTGTGTC CCTATTTAAA ACAATGCAGG CGTCTCTGGT GTACCAGCGC AGAAGGAGTC 
CACAAGGGCT GTCGCACTCA ACACATGCCT CTGGCAGACG GAACAAACTG TGGTCCTGGG 
ATGCATTGCC ACCATGGGCT ATGTGTAAAT AAAGAAATGG AGACACGTCC TGTAGATGGA 
GATTGGGGAC CATGGGGACC TTACAGTTCT TGTTCAAGAA CATGTGGAGG TGGAATCAAA 
AGCACAAGCA GGCTCTGTAA TCGCCCCGAG CCAAGAAACG GAGGCAAGTA CTGTGTGGGC 
CGCAGGATGA AATTTCGATC ATGTAACACT GATTCATGTC CAAAAGGCAA GCAAGACTTC 
CGTGAGAAGC AGTGCTCTGA TTTTGATGGT AAACATTTCA ACATCAATGG TCTCTCCCCT 
AACGTGAGGT GGCTTCCAAA GTACAGTGGG ATTGCTGTAA AAGATCGTTG TAAACTCTAT 
TGTCGGGTGG CTGGAACCAC TAGCTTCTAC CAACTGAAGG ACAGGGTTGC CGATGGTACT 
CCTTGTGGAA CGGAAACAAA TGACATCTGT GTTCAAGGCC TGTGTCGGCA AGCTGGCTGT 
GATCACGTGT TAAACTCCAA GGCCAGGAGA GACAAATGTG GCGTGTGTGG TGGGGATAAC 
TCCTCCTGCA GGACAATGGC AGGCGTCTTC AACAGTGCGC ATTATGGTTA TAATGTCGTT 
GTAAAGATTC CCCCAGGAGC AACAAACATT GATATTCTTC AGCACAGCTA TTCTGGAAAA 
CCAGAAGATG ACAATTACCT GGCATTATCG GACACTCAAG GGAATTTTCT TTTGAATGGG 
AATTTTGTTG TAAGTATGTC CAAACGGGAA ATCAACGTAC AAGGAGCTAT TTTTGAATAC 
AGTGGTTCAA ACAACTCAAT TGAAAGGATT AATAGTACTG ATCGACTGGA AGAAGAACTT 
GTTTTGCAGG TTTTGTGTGT GGGTAATCTA TACAACCCTG ATGTACGTTA TTCCTTCAAT 
ATCCCCGTGG AAGAGAAGAG TGATCTATTC ACATGGGACC CGTATGGACC ATGGCAAGAC 
TGTACAAAAA TGTGTCAAGG TCTTCATAGA AGAAAAATTA CTTGTGTACG TAGGAGTGAT 
CATATGGTCA TGTCTGATCA AAGCTGTGAC CACTTACCAC TTCCATTGCT GGTAACTGAA 
AGGTGCAATA CAGACTGTGA ACTCAGGTGG CACATCATTG GCAAAAGTGA ATGTTCAACC 
CTTTGTGGTC AAGGATACAA GTCATTGGAC GTCCATTGTA TGAAGTATTC CATTCATAAA 
GGACAGACTG TTCCAGTAGA TGACCACTAC TGCAGTGACC AACTTAAACC TCCAACCCGA 
GAACCCTGCC ATGGTGACTG TGTCTTAACA AGGTGGCATT ATTTAGAATG GTCTCAGTGT 
TCCAGGAGTT GCGGAGGAGG GGAGAGGTCT CGAGAATCTT ACTGTATAAA TAACTTTGGC 
CACCGTCTTG CTGACAGAGA ATGCCAGGAA CTTCCCCGAG TGACAACAGA GAATTGCAAT 
GAATTTTCCT GTCCCAGTTG GGCCACTAGT GAGTGGAGTG AGTGTCTGGT TACCTGTGGA 
AAAGGAACAA AGCAGCGGCA GGTGTGGTGT CACCTGAATG AAGATCACTT GAGTGATGGC 
TTCTGTGACC CAAGCACCAA GCCTGAGTCT CAGAGACCCT GTGAGCTTCA TGCATGTACT 
TCCTGGCAAG TAGGACCTTG GGGTTCTTGC ACAGCCACGT GTGGACAGGG GTACCAGATG 
CGTGCTGTGA AGTGTGTCAA CGAGCTATTT AGCACAGTGC TAGATGACAG ACTGTGCCAT 
GGAGCTAGTC GCCCAAGTGA AAGGCAGAAT TGTATTGTTA CACCTTGCCC AGTTATCCCT 
AAAATTGGGG CCACTTCCTT GCCAGCTGTT CCCATGAGAA AGATAACACA ATGGCGATAT 
GGTTCTTGGA CACCATGCTC CGTGTCTTGT GGAAGAGGAA ATCAAGCCCG CTATGTAAGT 
TGTCGTGATG CTCATGATGG AATAGCAGAT GAATCATTTT GTGCCCACTT ACCCCGACCG 
GCTGAAATAG CTGTCTGTTT TAGCCCTTGT GGAGAGTGGC AAACAGGGAA TTGGTCACCT 
TGTTCAGCTT CCTGTGGCCG TGGAAAAATG ACTCGACAAG TTTTATGCAT CAACTATCAT 
CAGCCAGTTA ATGAGAATTA CTGTGACCCT GAAGTCCGCC CTGTGATAGA ACAAGAATGT 
AATCTGGCAG CCTGCCCTCC CACGTACAGC CACTTTCCGA GTTCCTCTGA GCAGCCAAGC 
CATTTTCCAG GCAGGAATTT TCCATTAACT CACAAACCAG AAGATAATCA AAATCAGAGG 
GTCCATCCAT CAATCAGAGA AAACCAGTGG AGAACAGGAC CATGGGGATC ATGCTCTAGC 
AGTTGTGCTG GAGGTGTTCA GCGTAGAGTT GTGGTCTGCC AGGATGAAAA TGGACAAAGT 
GCCAGTTACT GTGATGCAGC GTCCAAACCC CCAGAGTCAA AGCACTGTGA TTCAGGACCT 
TGTCCACAGT GGAACTATGG AAGCTGGGGA GAATGTACGC AAACGTGTGG AGGAGGAATA 
AAATCAAGAT TTGTAATATG CCAGTTTCCC GATGGCCAGC TGTCACAAGA GCAAAACTGT 
GAAATGTTGA ACAAGCCGCC TAGTGTGGTA CAGTGTCATA TACATGCTTG CCCTGATGAC 
GTATCATGGC ATCGGGGACC ATGGAAATCG TGCTCAGCTT CCTGTGGTAA AGGTTTAAAA 
TACCGTGAGG TGCTTTGCAT TGACCAATTC CACAGGAAAT TAGAAGAAAA ATATTGCAGT 
CATCTACAGA AACCTCGAAC ACATAAAGCT TGTAGATCTG TCCGATGCCC TTCATGGAAA 
GCCAACAGAT GGAAGGAGTG CTCTGTGACC TGCGGCTCCG GAGTTCAGCA GAGGGATGTG 
TACTGCAGAC TCAGAGGAGT TGGTCCGGTG GCGGAAGAAA AGTGTGATCC ATCCACGCGG 
CCTTATTTTC AGAGAGAGTG TTGGCATCGG GACTGCATAC AGCACCACTG GGCGGCAGGA 
GAGTGGTTGG ATTGCTCAGT ATCGTGTAAG AAAAGAGAGA CATATCGGCA AGTGAAGTGC 
ATGGATGCAC AAAACATTCA AGTGAGTGAG AGTTTCTGTG ACCTCGCGAC CAGACCTCTC 
GCGGTGAAAA AGTGCAGAAA CTCTCCCTGC AAGTATATCG TGGTAACAGG AGACTCATCC 
CAGTGTGCAG GTAACTGTGG ATTTAGTTAT CGACAAAGGA TTACATATTG CGTTGGGGTC 
CAATCTATGG AGAAACATAA ACTTCATGAG CTATGGCCTA TAGACTATCG AGTATGCCCT 
GTGCTGCCTT CCCCTCAGGT TTACAAATGC AATTTTAGGA CCTGTTTGCA TATGGCCACT 
TGGAAAGTTG GAAAATGGAG CAAGTGCTCT GTGACTTGTG GAGTTGGGAT GATGGAGAGA 
AGAGTGGAAT GCATGGCTGA TAATGGTCTG TCCAGTGATT TATGTTTAAA GCATTTAAAA 
CCAGATGCTC AAAAGAAATG TTATGTCAGT GACTGTAAAA CATTTATGAG CTGTAAAGAA 
ATACAAGTGA AAAACAACAT TACCAAGGAT GGTGACTATT ACCTTAACAT TAAAGGAAGA 
ATAACAAAGA TCTATTGTGC TGGCATGCAC TTGGAGAACC CTAAGGAATA TTTATCATTG 
GTCAAAGGTG AAGAAGACAA CTTTTCTGAA GTGTATGGTT TTAGACTACG AAATCCATAT 
GAATGTCCTT TTAATGGAAG CAGGAGGCAT GACTGTGAAT GTAAAAATGA CTATCTGGCT 
GCTGGACACA CTGTTTTTAG CAAAATAAGA ATTGATCTTA CTTCCATGCA AATTAAAACT 
ACAGACCTTC TTTTTGCCCA GACAATATTT GGAAATGCAG TTCCATTTGC CACAGCTGGA 
GATTGCTATA GTGCTGCCAG ATGCCCACAG GGACAGTTCA GCATTAATTT GGCAGGAACT 
GGGATGAAGA TATCCAGTAC AGCAAAGTGG CTGGCTCAGG GCAGTTATGC CTCTGTGATC 
ATTCACAGAT CACAGGATGG AACCAAAGTC TATGGCAGAT GTGGAGGGTT CTGTGGAAAG 
TGTATTCCTC ACATGACTAC TGGTCTCCCA ATTCAAGTAG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012029852.1
CDS	718..5382
Translation	MSLVMCQTGYTDQQTKQRTRNLELFSFIYCIGLYDKHFWPLSLVILCMTRFNVPHDDSFKCKETGIKHQYHVMAPTLNYHTSPWTWSKCSQKYITEFLDTGHGECLLDKPNGRIYDLSSQLPGLMYDVNKQCELMFGPGSQVCPYLKQCRRLWCTSAEGVHKGCRTQHMPLADGTNCGPGMHCHHGLCVNKEMETRPVDGDWGPWGPYSSCSRTCGGGIKSTSRLCNRPEPRNGGKYCVGRRMKFRSCNTDSCPKGKQDFREKQCSDFDGKHFNINGLSPNVRWLPKYSGIAVKDRCKLYCRVAGTTSFYQLKDRVADGTPCGTETNDICVQGLCRQAGCDHVLNSKARRDKCGVCGGDNSSCRTMAGVFNSAHYGYNVVVKIPPGATNIDILQHSYSGKPEDDNYLALSDTQGNFLLNGNFVVSMSKREINVQGAIFEYSGSNNSIERINSTDRLEEELVLQVLCVGNLYNPDVRYSFNIPVEEKSDLFTWDPYGPWQDCTKMCQGLHRRKITCVRRSDHMVMSDQSCDHLPLPLLVTERCNTDCELRWHIIGKSECSTLCGQGYKSLDVHCMKYSIHKGQTVPVDDHYCSDQLKPPTREPCHGDCVLTRWHYLEWSQCSRSCGGGERSRESYCINNFGHRLADRECQELPRVTTENCNEFSCPSWATSEWSECLVTCGKGTKQRQVWCHLNEDHLSDGFCDPSTKPESQRPCELHACTSWQVGPWGSCTATCGQGYQMRAVKCVNELFSTVLDDRLCHGASRPSERQNCIVTPCPVIPKIGATSLPAVPMRKITQWRYGSWTPCSVSCGRGNQARYVSCRDAHDGIADESFCAHLPRPAEIAVCFSPCGEWQTGNWSPCSASCGRGKMTRQVLCINYHQPVNENYCDPEVRPVIEQECNLAACPPTYSHFPSSSEQPSHFPGRNFPLTHKPEDNQNQRVHPSIRENQWRTGPWGSCSSSCAGGVQRRVVVCQDENGQSASYCDAASKPPESKHCDSGPCPQWNYGSWGECTQTCGGGIKSRFVICQFPDGQLSQEQNCEMLNKPPSVVQCHIHACPDDVSWHRGPWKSCSASCGKGLKYREVLCIDQFHRKLEEKYCSHLQKPRTHKACRSVRCPSWKANRWKECSVTCGSGVQQRDVYCRLRGVGPVAEEKCDPSTRPYFQRECWHRDCIQHHWAAGEWLDCSVSCKKRETYRQVKCMDAQNIQVSESFCDLATRPLAVKKCRNSPCKYIVVTGDSSQCAGNCGFSYRQRITYCVGVQSMEKHKLHELWPIDYRVCPVLPSPQVYKCNFRTCLHMATWKVGKWSKCSVTCGVGMMERRVECMADNGLSSDLCLKHLKPDAQKKCYVSDCKTFMSCKEIQVKNNITKDGDYYLNIKGRITKIYCAGMHLENPKEYLSLVKGEEDNFSEVYGFRLRNPYECPFNGSRRHDCECKNDYLAAGHTVFSKIRIDLTSMQIKTTDLLFAQTIFGNAVPFATAGDCYSAARCPQGQFSINLAGTGMKISSTAKWLAQGSYASVIIHRSQDGTKVYGRCGGFCGKCIPHMTTGLPIQVV

Target ORF information:

RefSeq Version	XM_012174462.2
Organism	Ovis aries(sheep)
Definition	Ovis aries ADAM metallopeptidase with thrombospondin type 1 motif 20 (ADAMTS20), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012174462.2

ORF Insert Sequence:

ATGAGCTTGG TCATGTGTCA GACAGGCTAC ACAGATCAAC AGACTAAGCA AAGAACTAGA 
AATTTGGAAC TCTTTTCTTT CATTTACTGT ATTGGATTAT ATGACAAGCA CTTCTGGCCT 
CTATCCCTTG TTATCCTATG CATGACCAGA TTTAATGTTC CACACGATGA TAGTTTTAAA 
TGTAAGGAAA CTGGAATTAA ACATCAGTAT CATGTCATGG CTCCAACTTT AAATTACCAC 
ACAAGTCCTT GGACCTGGTC AAAATGTAGT CAGAAATATA TCACTGAATT CCTAGACACC 
GGTCATGGGG AATGCCTCCT TGACAAACCA AATGGAAGAA TATACGATTT GTCTTCACAG 
CTTCCTGGAT TAATGTATGA TGTAAACAAG CAATGTGAGC TTATGTTTGG TCCTGGATCA 
CAAGTGTGTC CCTATTTAAA ACAATGCAGG CGTCTCTGGT GTACCAGCGC AGAAGGAGTC 
CACAAGGGCT GTCGCACTCA ACACATGCCT CTGGCAGACG GAACAAACTG TGGTCCTGGG 
ATGCATTGCC ACCATGGGCT ATGTGTAAAT AAAGAAATGG AGACACGTCC TGTAGATGGA 
GATTGGGGAC CATGGGGACC TTACAGTTCT TGTTCAAGAA CATGTGGAGG TGGAATCAAA 
AGCACAAGCA GGCTCTGTAA TCGCCCCGAG CCAAGAAACG GAGGCAAGTA CTGTGTGGGC 
CGCAGGATGA AATTTCGATC ATGTAACACT GATTCATGTC CAAAAGGCAA GCAAGACTTC 
CGTGAGAAGC AGTGCTCTGA TTTTGATGGT AAACATTTCA ACATCAATGG TCTCTCCCCT 
AACGTGAGGT GGCTTCCAAA GTACAGTGGG ATTGCTGTAA AAGATCGTTG TAAACTCTAT 
TGTCGGGTGG CTGGAACCAC TAGCTTCTAC CAACTGAAGG ACAGGGTTGC CGATGGTACT 
CCTTGTGGAA CGGAAACAAA TGACATCTGT GTTCAAGGCC TGTGTCGGCA AGCTGGCTGT 
GATCACGTGT TAAACTCCAA GGCCAGGAGA GACAAATGTG GCGTGTGTGG TGGGGATAAC 
TCCTCCTGCA GGACAATGGC AGGCGTCTTC AACAGTGCGC ATTATGGTTA TAATGTCGTT 
GTAAAGATTC CCCCAGGAGC AACAAACATT GATATTCTTC AGCACAGCTA TTCTGGAAAA 
CCAGAAGATG ACAATTACCT GGCATTATCG GACACTCAAG GGAATTTTCT TTTGAATGGG 
AATTTTGTTG TAAGTATGTC CAAACGGGAA ATCAACGTAC AAGGAGCTAT TTTTGAATAC 
AGTGGTTCAA ACAACTCAAT TGAAAGGATT AATAGTACTG ATCGACTGGA AGAAGAACTT 
GTTTTGCAGG TTTTGTGTGT GGGTAATCTA TACAACCCTG ATGTACGTTA TTCCTTCAAT 
ATCCCCGTGG AAGAGAAGAG TGATCTATTC ACATGGGACC CGTATGGACC ATGGCAAGAC 
TGTACAAAAA TGTGTCAAGG TCTTCATAGA AGAAAAATTA CTTGTGTACG TAGGAGTGAT 
CATATGGTCA TGTCTGATCA AAGCTGTGAC CACTTACCAC TTCCATTGCT GGTAACTGAA 
AGGTGCAATA CAGACTGTGA ACTCAGGTGG CACATCATTG GCAAAAGTGA ATGTTCAACC 
CTTTGTGGTC AAGGATACAA GTCATTGGAC GTCCATTGTA TGAAGTATTC CATTCATAAA 
GGACAGACTG TTCCAGTAGA TGACCACTAC TGCAGTGACC AACTTAAACC TCCAACCCGA 
GAACCCTGCC ATGGTGACTG TGTCTTAACA AGGTGGCATT ATTTAGAATG GTCTCAGTGT 
TCCAGGAGTT GCGGAGGAGG GGAGAGGTCT CGAGAATCTT ACTGTATAAA TAACTTTGGC 
CACCGTCTTG CTGACAGAGA ATGCCAGGAA CTTCCCCGAG TGACAACAGA GAATTGCAAT 
GAATTTTCCT GTCCCAGTTG GGCCACTAGT GAGTGGAGTG AGTGTCTGGT TACCTGTGGA 
AAAGGAACAA AGCAGCGGCA GGTGTGGTGT CACCTGAATG AAGATCACTT GAGTGATGGC 
TTCTGTGACC CAAGCACCAA GCCTGAGTCT CAGAGACCCT GTGAGCTTCA TGCATGTACT 
TCCTGGCAAG TAGGACCTTG GGGTTCTTGC ACAGCCACGT GTGGACAGGG GTACCAGATG 
CGTGCTGTGA AGTGTGTCAA CGAGCTATTT AGCACAGTGC TAGATGACAG ACTGTGCCAT 
GGAGCTAGTC GCCCAAGTGA AAGGCAGAAT TGTATTGTTA CACCTTGCCC AGTTATCCCT 
AAAATTGGGG CCACTTCCTT GCCAGCTGTT CCCATGAGAA AGATAACACA ATGGCGATAT 
GGTTCTTGGA CACCATGCTC CGTGTCTTGT GGAAGAGGAA ATCAAGCCCG CTATGTAAGT 
TGTCGTGATG CTCATGATGG AATAGCAGAT GAATCATTTT GTGCCCACTT ACCCCGACCG 
GCTGAAATAG CTGTCTGTTT TAGCCCTTGT GGAGAGTGGC AAACAGGGAA TTGGTCACCT 
TGTTCAGCTT CCTGTGGCCG TGGAAAAATG ACTCGACAAG TTTTATGCAT CAACTATCAT 
CAGCCAGTTA ATGAGAATTA CTGTGACCCT GAAGTCCGCC CTGTGATAGA ACAAGAATGT 
AATCTGGCAG CCTGCCCTCC CACGTACAGC CACTTTCCGA GTTCCTCTGA GCAGCCAAGC 
CATTTTCCAG GCAGGAATTT TCCATTAACT CACAAACCAG AAGATAATCA AAATCAGAGG 
GTCCATCCAT CAATCAGAGA AAACCAGTGG AGAACAGGAC CATGGGGATC ATGCTCTAGC 
AGTTGTGCTG GAGGTGTTCA GCGTAGAGTT GTGGTCTGCC AGGATGAAAA TGGACAAAGT 
GCCAGTTACT GTGATGCAGC GTCCAAACCC CCAGAGTCAA AGCACTGTGA TTCAGGACCT 
TGTCCACAGT GGAACTATGG AAGCTGGGGA GAATGTACGC AAACGTGTGG AGGAGGAATA 
AAATCAAGAT TTGTAATATG CCAGTTTCCC GATGGCCAGC TGTCACAAGA GCAAAACTGT 
GAAATGTTGA ACAAGCCGCC TAGTGTGGTA CAGTGTCATA TACATGCTTG CCCTGATGAC 
GTATCATGGC ATCGGGGACC ATGGAAATCG TGCTCAGCTT CCTGTGGTAA AGGTTTAAAA 
TACCGTGAGG TGCTTTGCAT TGACCAATTC CACAGGAAAT TAGAAGAAAA ATATTGCAGT 
CATCTACAGA AACCTCGAAC ACATAAAGCT TGTAGATCTG TCCGATGCCC TTCATGGAAA 
GCCAACAGAT GGAAGGAGTG CTCTGTGACC TGCGGCTCCG GAGTTCAGCA GAGGGATGTG 
TACTGCAGAC TCAGAGGAGT TGGTCCGGTG GCGGAAGAAA AGTGTGATCC ATCCACGCGG 
CCTTATTTTC AGAGAGAGTG TTGGCATCGG GACTGCATAC AGCACCACTG GGCGGCAGGA 
GAGTGGTTGG ATTGCTCAGT ATCGTGTAAG AAAAGAGAGA CATATCGGCA AGTGAAGTGC 
ATGGATGCAC AAAACATTCA AGTGAGTGAG AGTTTCTGTG ACCTCGCGAC CAGACCTCTC 
GCGGTGAAAA AGTGCAGAAA CTCTCCCTGC AAGTATATCG TGGTAACAGG AGACTCATCC 
CAGTGTGCAG GTAACTGTGG ATTTAGTTAT CGACAAAGGA TTACATATTG CGTTGGGGTC 
CAATCTATGG AGAAACATAA ACTTCATGAG CTATGGCCTA TAGACTATCG AGTATGCCCT 
GTGCTGCCTT CCCCTCAGGT TTACAAATGC AATTTTAGGA CCTGTTTGCA TATGGCCACT 
TGGAAAGTTG GAAAATGGAG CAAGTGCTCT GTGACTTGTG GAGTTGGGAT GATGGAGAGA 
AGAGTGGAAT GCATGGCTGA TAATGGTCTG TCCAGTGATT TATGTTTAAA GCATTTAAAA 
CCAGATGCTC AAAAGAAATG TTATGTCAGT GACTGTAAAA CATTTATGAG CTGTAAAGAA 
ATACAAGTGA AAAACAACAT TACCAAGGAT GGTGACTATT ACCTTAACAT TAAAGGAAGA 
ATAACAAAGA TCTATTGTGC TGGCATGCAC TTGGAGAACC CTAAGGAATA TTTATCATTG 
GTCAAAGGTG AAGAAGACAA CTTTTCTGAA GTGTATGGTT TTAGACTACG AAATCCATAT 
GAATGTCCTT TTAATGGAAG CAGGAGGCAT GACTGTGAAT GTAAAAATGA CTATCTGGCT 
GCTGGACACA CTGTTTTTAG CAAAATAAGA ATTGATCTTA CTTCCATGCA AATTAAAACT 
ACAGACCTTC TTTTTGCCCA GACAATATTT GGAAATGCAG TTCCATTTGC CACAGCTGGA 
GATTGCTATA GTGCTGCCAG ATGCCCACAG GGACAGTTCA GCATTAATTT GGCAGGAACT 
GGGATGAAGA TATCCAGTAC AGCAAAGTGG CTGGCTCAGG GCAGTTATGC CTCTGTGATC 
ATTCACAGAT CACAGGATGG AACCAAAGTC TATGGCAGAT GTGGAGGGTT CTGTGGAAAG 
TGTATTCCTC ACATGACTAC TGGTCTCCCA ATTCAAGTAG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ADAMTS20 ( XM_015094726.1 ) cDNA ORF clone, Ovis aries musimon(Ovis orientalis musimon) -> XP_014950212.1 Ovis aries ADAM metallopeptidase with thrombospondin type 1 motif 20 (ADAMTS20), transcript variant X3, mRNA.

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CloneID	OSm36428
Clone ID Related Accession (Same CDS sequence)	XM_012174462.2 , XM_015094726.1
Accession Version	XM_015094726.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4665bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries(sheep)
Product	A disintegrin and metalloproteinase with thrombospondin motifs 20 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014639012.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTTGG TCATGTGTCA GACAGGCTAC ACAGATCAAC AGACTAAGCA AAGAACTAGA 
AATTTGGAAC TCTTTTCTTT CATTTACTGT ATTGGATTAT ATGACAAGCA CTTCTGGCCT 
CTATCCCTTG TTATCCTATG CATGACCAGA TTTAATGTTC CACACGATGA TAGTTTTAAA 
TGTAAGGAAA CTGGAATTAA ACATCAGTAT CATGTCATGG CTCCAACTTT AAATTACCAC 
ACAAGTCCTT GGACCTGGTC AAAATGTAGT CAGAAATATA TCACTGAATT CCTAGACACC 
GGTCATGGGG AATGCCTCCT TGACAAACCA AATGGAAGAA TATACGATTT GTCTTCACAG 
CTTCCTGGAT TAATGTATGA TGTAAACAAG CAATGTGAGC TTATGTTTGG TCCTGGATCA 
CAAGTGTGTC CCTATTTAAA ACAATGCAGG CGTCTCTGGT GTACCAGCGC AGAAGGAGTC 
CACAAGGGCT GTCGCACTCA ACACATGCCT CTGGCAGACG GAACAAACTG TGGTCCTGGG 
ATGCATTGCC ACCATGGGCT ATGTGTAAAT AAAGAAATGG AGACACGTCC TGTAGATGGA 
GATTGGGGAC CATGGGGACC TTACAGTTCT TGTTCAAGAA CATGTGGAGG TGGAATCAAA 
AGCACAAGCA GGCTCTGTAA TCGCCCCGAG CCAAGAAACG GAGGCAAGTA CTGTGTGGGC 
CGCAGGATGA AATTTCGATC ATGTAACACT GATTCATGTC CAAAAGGCAA GCAAGACTTC 
CGTGAGAAGC AGTGCTCTGA TTTTGATGGT AAACATTTCA ACATCAATGG TCTCTCCCCT 
AACGTGAGGT GGCTTCCAAA GTACAGTGGG ATTGCTGTAA AAGATCGTTG TAAACTCTAT 
TGTCGGGTGG CTGGAACCAC TAGCTTCTAC CAACTGAAGG ACAGGGTTGC CGATGGTACT 
CCTTGTGGAA CGGAAACAAA TGACATCTGT GTTCAAGGCC TGTGTCGGCA AGCTGGCTGT 
GATCACGTGT TAAACTCCAA GGCCAGGAGA GACAAATGTG GCGTGTGTGG TGGGGATAAC 
TCCTCCTGCA GGACAATGGC AGGCGTCTTC AACAGTGCGC ATTATGGTTA TAATGTCGTT 
GTAAAGATTC CCCCAGGAGC AACAAACATT GATATTCTTC AGCACAGCTA TTCTGGAAAA 
CCAGAAGATG ACAATTACCT GGCATTATCG GACACTCAAG GGAATTTTCT TTTGAATGGG 
AATTTTGTTG TAAGTATGTC CAAACGGGAA ATCAACGTAC AAGGAGCTAT TTTTGAATAC 
AGTGGTTCAA ACAACTCAAT TGAAAGGATT AATAGTACTG ATCGACTGGA AGAAGAACTT 
GTTTTGCAGG TTTTGTGTGT GGGTAATCTA TACAACCCTG ATGTACGTTA TTCCTTCAAT 
ATCCCCGTGG AAGAGAAGAG TGATCTATTC ACATGGGACC CGTATGGACC ATGGCAAGAC 
TGTACAAAAA TGTGTCAAGG TCTTCATAGA AGAAAAATTA CTTGTGTACG TAGGAGTGAT 
CATATGGTCA TGTCTGATCA AAGCTGTGAC CACTTACCAC TTCCATTGCT GGTAACTGAA 
AGGTGCAATA CAGACTGTGA ACTCAGGTGG CACATCATTG GCAAAAGTGA ATGTTCAACC 
CTTTGTGGTC AAGGATACAA GTCATTGGAC GTCCATTGTA TGAAGTATTC CATTCATAAA 
GGACAGACTG TTCCAGTAGA TGACCACTAC TGCAGTGACC AACTTAAACC TCCAACCCGA 
GAACCCTGCC ATGGTGACTG TGTCTTAACA AGGTGGCATT ATTTAGAATG GTCTCAGTGT 
TCCAGGAGTT GCGGAGGAGG GGAGAGGTCT CGAGAATCTT ACTGTATAAA TAACTTTGGC 
CACCGTCTTG CTGACAGAGA ATGCCAGGAA CTTCCCCGAG TGACAACAGA GAATTGCAAT 
GAATTTTCCT GTCCCAGTTG GGCCACTAGT GAGTGGAGTG AGTGTCTGGT TACCTGTGGA 
AAAGGAACAA AGCAGCGGCA GGTGTGGTGT CACCTGAATG AAGATCACTT GAGTGATGGC 
TTCTGTGACC CAAGCACCAA GCCTGAGTCT CAGAGACCCT GTGAGCTTCA TGCATGTACT 
TCCTGGCAAG TAGGACCTTG GGGTTCTTGC ACAGCCACGT GTGGACAGGG GTACCAGATG 
CGTGCTGTGA AGTGTGTCAA CGAGCTATTT AGCACAGTGC TAGATGACAG ACTGTGCCAT 
GGAGCTAGTC GCCCAAGTGA AAGGCAGAAT TGTATTGTTA CACCTTGCCC AGTTATCCCT 
AAAATTGGGG CCACTTCCTT GCCAGCTGTT CCCATGAGAA AGATAACACA ATGGCGATAT 
GGTTCTTGGA CACCATGCTC CGTGTCTTGT GGAAGAGGAA ATCAAGCCCG CTATGTAAGT 
TGTCGTGATG CTCATGATGG AATAGCAGAT GAATCATTTT GTGCCCACTT ACCCCGACCG 
GCTGAAATAG CTGTCTGTTT TAGCCCTTGT GGAGAGTGGC AAACAGGGAA TTGGTCACCT 
TGTTCAGCTT CCTGTGGCCG TGGAAAAATG ACTCGACAAG TTTTATGCAT CAACTATCAT 
CAGCCAGTTA ATGAGAATTA CTGTGACCCT GAAGTCCGCC CTGTGATAGA ACAAGAATGT 
AATCTGGCAG CCTGCCCTCC CACGTACAGC CACTTTCCGA GTTCCTCTGA GCAGCCAAGC 
CATTTTCCAG GCAGGAATTT TCCATTAACT CACAAACCAG AAGATAATCA AAATCAGAGG 
GTCCATCCAT CAATCAGAGA AAACCAGTGG AGAACAGGAC CATGGGGATC ATGCTCTAGC 
AGTTGTGCTG GAGGTGTTCA GCGTAGAGTT GTGGTCTGCC AGGATGAAAA TGGACAAAGT 
GCCAGTTACT GTGATGCAGC GTCCAAACCC CCAGAGTCAA AGCACTGTGA TTCAGGACCT 
TGTCCACAGT GGAACTATGG AAGCTGGGGA GAATGTACGC AAACGTGTGG AGGAGGAATA 
AAATCAAGAT TTGTAATATG CCAGTTTCCC GATGGCCAGC TGTCACAAGA GCAAAACTGT 
GAAATGTTGA ACAAGCCGCC TAGTGTGGTA CAGTGTCATA TACATGCTTG CCCTGATGAC 
GTATCATGGC ATCGGGGACC ATGGAAATCG TGCTCAGCTT CCTGTGGTAA AGGTTTAAAA 
TACCGTGAGG TGCTTTGCAT TGACCAATTC CACAGGAAAT TAGAAGAAAA ATATTGCAGT 
CATCTACAGA AACCTCGAAC ACATAAAGCT TGTAGATCTG TCCGATGCCC TTCATGGAAA 
GCCAACAGAT GGAAGGAGTG CTCTGTGACC TGCGGCTCCG GAGTTCAGCA GAGGGATGTG 
TACTGCAGAC TCAGAGGAGT TGGTCCGGTG GCGGAAGAAA AGTGTGATCC ATCCACGCGG 
CCTTATTTTC AGAGAGAGTG TTGGCATCGG GACTGCATAC AGCACCACTG GGCGGCAGGA 
GAGTGGTTGG ATTGCTCAGT ATCGTGTAAG AAAAGAGAGA CATATCGGCA AGTGAAGTGC 
ATGGATGCAC AAAACATTCA AGTGAGTGAG AGTTTCTGTG ACCTCGCGAC CAGACCTCTC 
GCGGTGAAAA AGTGCAGAAA CTCTCCCTGC AAGTATATCG TGGTAACAGG AGACTCATCC 
CAGTGTGCAG GTAACTGTGG ATTTAGTTAT CGACAAAGGA TTACATATTG CGTTGGGGTC 
CAATCTATGG AGAAACATAA ACTTCATGAG CTATGGCCTA TAGACTATCG AGTATGCCCT 
GTGCTGCCTT CCCCTCAGGT TTACAAATGC AATTTTAGGA CCTGTTTGCA TATGGCCACT 
TGGAAAGTTG GAAAATGGAG CAAGTGCTCT GTGACTTGTG GAGTTGGGAT GATGGAGAGA 
AGAGTGGAAT GCATGGCTGA TAATGGTCTG TCCAGTGATT TATGTTTAAA GCATTTAAAA 
CCAGATGCTC AAAAGAAATG TTATGTCAGT GACTGTAAAA CATTTATGAG CTGTAAAGAA 
ATACAAGTGA AAAACAACAT TACCAAGGAT GGTGACTATT ACCTTAACAT TAAAGGAAGA 
ATAACAAAGA TCTATTGTGC TGGCATGCAC TTGGAGAACC CTAAGGAATA TTTATCATTG 
GTCAAAGGTG AAGAAGACAA CTTTTCTGAA GTGTATGGTT TTAGACTACG AAATCCATAT 
GAATGTCCTT TTAATGGAAG CAGGAGGCAT GACTGTGAAT GTAAAAATGA CTATCTGGCT 
GCTGGACACA CTGTTTTTAG CAAAATAAGA ATTGATCTTA CTTCCATGCA AATTAAAACT 
ACAGACCTTC TTTTTGCCCA GACAATATTT GGAAATGCAG TTCCATTTGC CACAGCTGGA 
GATTGCTATA GTGCTGCCAG ATGCCCACAG GGACAGTTCA GCATTAATTT GGCAGGAACT 
GGGATGAAGA TATCCAGTAC AGCAAAGTGG CTGGCTCAGG GCAGTTATGC CTCTGTGATC 
ATTCACAGAT CACAGGATGG AACCAAAGTC TATGGCAGAT GTGGAGGGTT CTGTGGAAAG 
TGTATTCCTC ACATGACTAC TGGTCTCCCA ATTCAAGTAG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014950212.1
CDS	4990..9654
Translation	MSLVMCQTGYTDQQTKQRTRNLELFSFIYCIGLYDKHFWPLSLVILCMTRFNVPHDDSFKCKETGIKHQYHVMAPTLNYHTSPWTWSKCSQKYITEFLDTGHGECLLDKPNGRIYDLSSQLPGLMYDVNKQCELMFGPGSQVCPYLKQCRRLWCTSAEGVHKGCRTQHMPLADGTNCGPGMHCHHGLCVNKEMETRPVDGDWGPWGPYSSCSRTCGGGIKSTSRLCNRPEPRNGGKYCVGRRMKFRSCNTDSCPKGKQDFREKQCSDFDGKHFNINGLSPNVRWLPKYSGIAVKDRCKLYCRVAGTTSFYQLKDRVADGTPCGTETNDICVQGLCRQAGCDHVLNSKARRDKCGVCGGDNSSCRTMAGVFNSAHYGYNVVVKIPPGATNIDILQHSYSGKPEDDNYLALSDTQGNFLLNGNFVVSMSKREINVQGAIFEYSGSNNSIERINSTDRLEEELVLQVLCVGNLYNPDVRYSFNIPVEEKSDLFTWDPYGPWQDCTKMCQGLHRRKITCVRRSDHMVMSDQSCDHLPLPLLVTERCNTDCELRWHIIGKSECSTLCGQGYKSLDVHCMKYSIHKGQTVPVDDHYCSDQLKPPTREPCHGDCVLTRWHYLEWSQCSRSCGGGERSRESYCINNFGHRLADRECQELPRVTTENCNEFSCPSWATSEWSECLVTCGKGTKQRQVWCHLNEDHLSDGFCDPSTKPESQRPCELHACTSWQVGPWGSCTATCGQGYQMRAVKCVNELFSTVLDDRLCHGASRPSERQNCIVTPCPVIPKIGATSLPAVPMRKITQWRYGSWTPCSVSCGRGNQARYVSCRDAHDGIADESFCAHLPRPAEIAVCFSPCGEWQTGNWSPCSASCGRGKMTRQVLCINYHQPVNENYCDPEVRPVIEQECNLAACPPTYSHFPSSSEQPSHFPGRNFPLTHKPEDNQNQRVHPSIRENQWRTGPWGSCSSSCAGGVQRRVVVCQDENGQSASYCDAASKPPESKHCDSGPCPQWNYGSWGECTQTCGGGIKSRFVICQFPDGQLSQEQNCEMLNKPPSVVQCHIHACPDDVSWHRGPWKSCSASCGKGLKYREVLCIDQFHRKLEEKYCSHLQKPRTHKACRSVRCPSWKANRWKECSVTCGSGVQQRDVYCRLRGVGPVAEEKCDPSTRPYFQRECWHRDCIQHHWAAGEWLDCSVSCKKRETYRQVKCMDAQNIQVSESFCDLATRPLAVKKCRNSPCKYIVVTGDSSQCAGNCGFSYRQRITYCVGVQSMEKHKLHELWPIDYRVCPVLPSPQVYKCNFRTCLHMATWKVGKWSKCSVTCGVGMMERRVECMADNGLSSDLCLKHLKPDAQKKCYVSDCKTFMSCKEIQVKNNITKDGDYYLNIKGRITKIYCAGMHLENPKEYLSLVKGEEDNFSEVYGFRLRNPYECPFNGSRRHDCECKNDYLAAGHTVFSKIRIDLTSMQIKTTDLLFAQTIFGNAVPFATAGDCYSAARCPQGQFSINLAGTGMKISSTAKWLAQGSYASVIIHRSQDGTKVYGRCGGFCGKCIPHMTTGLPIQVV

Target ORF information:

RefSeq Version	XM_015094726.1
Organism	Ovis aries(sheep)
Definition	Ovis aries ADAM metallopeptidase with thrombospondin type 1 motif 20 (ADAMTS20), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015094726.1

ORF Insert Sequence:

ATGAGCTTGG TCATGTGTCA GACAGGCTAC ACAGATCAAC AGACTAAGCA AAGAACTAGA 
AATTTGGAAC TCTTTTCTTT CATTTACTGT ATTGGATTAT ATGACAAGCA CTTCTGGCCT 
CTATCCCTTG TTATCCTATG CATGACCAGA TTTAATGTTC CACACGATGA TAGTTTTAAA 
TGTAAGGAAA CTGGAATTAA ACATCAGTAT CATGTCATGG CTCCAACTTT AAATTACCAC 
ACAAGTCCTT GGACCTGGTC AAAATGTAGT CAGAAATATA TCACTGAATT CCTAGACACC 
GGTCATGGGG AATGCCTCCT TGACAAACCA AATGGAAGAA TATACGATTT GTCTTCACAG 
CTTCCTGGAT TAATGTATGA TGTAAACAAG CAATGTGAGC TTATGTTTGG TCCTGGATCA 
CAAGTGTGTC CCTATTTAAA ACAATGCAGG CGTCTCTGGT GTACCAGCGC AGAAGGAGTC 
CACAAGGGCT GTCGCACTCA ACACATGCCT CTGGCAGACG GAACAAACTG TGGTCCTGGG 
ATGCATTGCC ACCATGGGCT ATGTGTAAAT AAAGAAATGG AGACACGTCC TGTAGATGGA 
GATTGGGGAC CATGGGGACC TTACAGTTCT TGTTCAAGAA CATGTGGAGG TGGAATCAAA 
AGCACAAGCA GGCTCTGTAA TCGCCCCGAG CCAAGAAACG GAGGCAAGTA CTGTGTGGGC 
CGCAGGATGA AATTTCGATC ATGTAACACT GATTCATGTC CAAAAGGCAA GCAAGACTTC 
CGTGAGAAGC AGTGCTCTGA TTTTGATGGT AAACATTTCA ACATCAATGG TCTCTCCCCT 
AACGTGAGGT GGCTTCCAAA GTACAGTGGG ATTGCTGTAA AAGATCGTTG TAAACTCTAT 
TGTCGGGTGG CTGGAACCAC TAGCTTCTAC CAACTGAAGG ACAGGGTTGC CGATGGTACT 
CCTTGTGGAA CGGAAACAAA TGACATCTGT GTTCAAGGCC TGTGTCGGCA AGCTGGCTGT 
GATCACGTGT TAAACTCCAA GGCCAGGAGA GACAAATGTG GCGTGTGTGG TGGGGATAAC 
TCCTCCTGCA GGACAATGGC AGGCGTCTTC AACAGTGCGC ATTATGGTTA TAATGTCGTT 
GTAAAGATTC CCCCAGGAGC AACAAACATT GATATTCTTC AGCACAGCTA TTCTGGAAAA 
CCAGAAGATG ACAATTACCT GGCATTATCG GACACTCAAG GGAATTTTCT TTTGAATGGG 
AATTTTGTTG TAAGTATGTC CAAACGGGAA ATCAACGTAC AAGGAGCTAT TTTTGAATAC 
AGTGGTTCAA ACAACTCAAT TGAAAGGATT AATAGTACTG ATCGACTGGA AGAAGAACTT 
GTTTTGCAGG TTTTGTGTGT GGGTAATCTA TACAACCCTG ATGTACGTTA TTCCTTCAAT 
ATCCCCGTGG AAGAGAAGAG TGATCTATTC ACATGGGACC CGTATGGACC ATGGCAAGAC 
TGTACAAAAA TGTGTCAAGG TCTTCATAGA AGAAAAATTA CTTGTGTACG TAGGAGTGAT 
CATATGGTCA TGTCTGATCA AAGCTGTGAC CACTTACCAC TTCCATTGCT GGTAACTGAA 
AGGTGCAATA CAGACTGTGA ACTCAGGTGG CACATCATTG GCAAAAGTGA ATGTTCAACC 
CTTTGTGGTC AAGGATACAA GTCATTGGAC GTCCATTGTA TGAAGTATTC CATTCATAAA 
GGACAGACTG TTCCAGTAGA TGACCACTAC TGCAGTGACC AACTTAAACC TCCAACCCGA 
GAACCCTGCC ATGGTGACTG TGTCTTAACA AGGTGGCATT ATTTAGAATG GTCTCAGTGT 
TCCAGGAGTT GCGGAGGAGG GGAGAGGTCT CGAGAATCTT ACTGTATAAA TAACTTTGGC 
CACCGTCTTG CTGACAGAGA ATGCCAGGAA CTTCCCCGAG TGACAACAGA GAATTGCAAT 
GAATTTTCCT GTCCCAGTTG GGCCACTAGT GAGTGGAGTG AGTGTCTGGT TACCTGTGGA 
AAAGGAACAA AGCAGCGGCA GGTGTGGTGT CACCTGAATG AAGATCACTT GAGTGATGGC 
TTCTGTGACC CAAGCACCAA GCCTGAGTCT CAGAGACCCT GTGAGCTTCA TGCATGTACT 
TCCTGGCAAG TAGGACCTTG GGGTTCTTGC ACAGCCACGT GTGGACAGGG GTACCAGATG 
CGTGCTGTGA AGTGTGTCAA CGAGCTATTT AGCACAGTGC TAGATGACAG ACTGTGCCAT 
GGAGCTAGTC GCCCAAGTGA AAGGCAGAAT TGTATTGTTA CACCTTGCCC AGTTATCCCT 
AAAATTGGGG CCACTTCCTT GCCAGCTGTT CCCATGAGAA AGATAACACA ATGGCGATAT 
GGTTCTTGGA CACCATGCTC CGTGTCTTGT GGAAGAGGAA ATCAAGCCCG CTATGTAAGT 
TGTCGTGATG CTCATGATGG AATAGCAGAT GAATCATTTT GTGCCCACTT ACCCCGACCG 
GCTGAAATAG CTGTCTGTTT TAGCCCTTGT GGAGAGTGGC AAACAGGGAA TTGGTCACCT 
TGTTCAGCTT CCTGTGGCCG TGGAAAAATG ACTCGACAAG TTTTATGCAT CAACTATCAT 
CAGCCAGTTA ATGAGAATTA CTGTGACCCT GAAGTCCGCC CTGTGATAGA ACAAGAATGT 
AATCTGGCAG CCTGCCCTCC CACGTACAGC CACTTTCCGA GTTCCTCTGA GCAGCCAAGC 
CATTTTCCAG GCAGGAATTT TCCATTAACT CACAAACCAG AAGATAATCA AAATCAGAGG 
GTCCATCCAT CAATCAGAGA AAACCAGTGG AGAACAGGAC CATGGGGATC ATGCTCTAGC 
AGTTGTGCTG GAGGTGTTCA GCGTAGAGTT GTGGTCTGCC AGGATGAAAA TGGACAAAGT 
GCCAGTTACT GTGATGCAGC GTCCAAACCC CCAGAGTCAA AGCACTGTGA TTCAGGACCT 
TGTCCACAGT GGAACTATGG AAGCTGGGGA GAATGTACGC AAACGTGTGG AGGAGGAATA 
AAATCAAGAT TTGTAATATG CCAGTTTCCC GATGGCCAGC TGTCACAAGA GCAAAACTGT 
GAAATGTTGA ACAAGCCGCC TAGTGTGGTA CAGTGTCATA TACATGCTTG CCCTGATGAC 
GTATCATGGC ATCGGGGACC ATGGAAATCG TGCTCAGCTT CCTGTGGTAA AGGTTTAAAA 
TACCGTGAGG TGCTTTGCAT TGACCAATTC CACAGGAAAT TAGAAGAAAA ATATTGCAGT 
CATCTACAGA AACCTCGAAC ACATAAAGCT TGTAGATCTG TCCGATGCCC TTCATGGAAA 
GCCAACAGAT GGAAGGAGTG CTCTGTGACC TGCGGCTCCG GAGTTCAGCA GAGGGATGTG 
TACTGCAGAC TCAGAGGAGT TGGTCCGGTG GCGGAAGAAA AGTGTGATCC ATCCACGCGG 
CCTTATTTTC AGAGAGAGTG TTGGCATCGG GACTGCATAC AGCACCACTG GGCGGCAGGA 
GAGTGGTTGG ATTGCTCAGT ATCGTGTAAG AAAAGAGAGA CATATCGGCA AGTGAAGTGC 
ATGGATGCAC AAAACATTCA AGTGAGTGAG AGTTTCTGTG ACCTCGCGAC CAGACCTCTC 
GCGGTGAAAA AGTGCAGAAA CTCTCCCTGC AAGTATATCG TGGTAACAGG AGACTCATCC 
CAGTGTGCAG GTAACTGTGG ATTTAGTTAT CGACAAAGGA TTACATATTG CGTTGGGGTC 
CAATCTATGG AGAAACATAA ACTTCATGAG CTATGGCCTA TAGACTATCG AGTATGCCCT 
GTGCTGCCTT CCCCTCAGGT TTACAAATGC AATTTTAGGA CCTGTTTGCA TATGGCCACT 
TGGAAAGTTG GAAAATGGAG CAAGTGCTCT GTGACTTGTG GAGTTGGGAT GATGGAGAGA 
AGAGTGGAAT GCATGGCTGA TAATGGTCTG TCCAGTGATT TATGTTTAAA GCATTTAAAA 
CCAGATGCTC AAAAGAAATG TTATGTCAGT GACTGTAAAA CATTTATGAG CTGTAAAGAA 
ATACAAGTGA AAAACAACAT TACCAAGGAT GGTGACTATT ACCTTAACAT TAAAGGAAGA 
ATAACAAAGA TCTATTGTGC TGGCATGCAC TTGGAGAACC CTAAGGAATA TTTATCATTG 
GTCAAAGGTG AAGAAGACAA CTTTTCTGAA GTGTATGGTT TTAGACTACG AAATCCATAT 
GAATGTCCTT TTAATGGAAG CAGGAGGCAT GACTGTGAAT GTAAAAATGA CTATCTGGCT 
GCTGGACACA CTGTTTTTAG CAAAATAAGA ATTGATCTTA CTTCCATGCA AATTAAAACT 
ACAGACCTTC TTTTTGCCCA GACAATATTT GGAAATGCAG TTCCATTTGC CACAGCTGGA 
GATTGCTATA GTGCTGCCAG ATGCCCACAG GGACAGTTCA GCATTAATTT GGCAGGAACT 
GGGATGAAGA TATCCAGTAC AGCAAAGTGG CTGGCTCAGG GCAGTTATGC CTCTGTGATC 
ATTCACAGAT CACAGGATGG AACCAAAGTC TATGGCAGAT GTGGAGGGTT CTGTGGAAAG 
TGTATTCCTC ACATGACTAC TGGTCTCCCA ATTCAAGTAG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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ADAMTS20 cDNA ORF clone, Ovis aries musimon(Ovis orientalis musimon)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Eメール

電話

FAX

オンラインフォーム

サポート

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