SPEF2 cDNA ORF clone, Ovis aries(sheep)

The following SPEF2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SPEF2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OOv13487 XM_015106493.1
Latest version!
Ovis aries musimon sperm flagellar 2 (SPEF2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OOv13487 XM_015106492.1
Latest version!
Ovis aries musimon sperm flagellar 2 (SPEF2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OOv13488 XM_012129859.2
Latest version!
Ovis aries musimon sperm flagellar 2 (SPEF2), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OOv13488 XM_012129857.2
Latest version!
Ovis aries musimon sperm flagellar 2 (SPEF2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OOv13489 XM_012129860.2
Latest version!
Ovis aries musimon sperm flagellar 2 (SPEF2), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OOv13490 XM_012129862.2
Latest version!
Ovis aries musimon sperm flagellar 2 (SPEF2), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OOv13491 XM_015106494.1
Latest version!
Ovis aries musimon sperm flagellar 2 (SPEF2), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OOv13492 XM_015106495.1
Latest version!
Ovis aries musimon sperm flagellar 2 (SPEF2), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OSm66603 XM_012097083.3
Latest version!
Ovis aries sperm flagellar protein 2 (LOC101111394), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OSm66604 XM_027980125.1
Latest version!
Ovis aries sperm flagellar protein 2 (LOC101111394), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OSm32427 XM_012097080.2
Latest version!
Ovis aries sperm flagellar 2 (SPEF2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OSm32427 XM_012097081.2
Latest version!
Ovis aries sperm flagellar 2 (SPEF2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OSm32428 XM_015101324.1
Latest version!
Ovis aries sperm flagellar 2 (SPEF2), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OSm32428 XM_012097082.2
Latest version!
Ovis aries sperm flagellar 2 (SPEF2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OSm32429 XM_012097083.2
Latest version!
Ovis aries sperm flagellar 2 (SPEF2), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OSm32430 XM_012097084.2
Latest version!
Ovis aries sperm flagellar 2 (SPEF2), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OSm32431 XM_012097085.2
Latest version!
Ovis aries sperm flagellar 2 (SPEF2), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OSm32432 XM_012097086.2
Latest version!
Ovis aries sperm flagellar 2 (SPEF2), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OOv13484 XM_015106490.1
Latest version!
Ovis aries musimon sperm flagellar 2 (SPEF2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
OOv13485 XM_015106491.1
Latest version!
Ovis aries musimon sperm flagellar 2 (SPEF2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
OOv13486 XM_012129853.2
Latest version!
Ovis aries musimon sperm flagellar 2 (SPEF2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
OSm32423 XM_012097074.2
Latest version!
Ovis aries sperm flagellar 2 (SPEF2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OSm32424 XM_012097076.2
Latest version!
Ovis aries sperm flagellar 2 (SPEF2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OSm32426 XM_012097079.2
Latest version!
Ovis aries sperm flagellar 2 (SPEF2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OOv13487
    Clone ID Related Accession (Same CDS sequence) XM_015106493.1 , XM_015106492.1
    Accession Version XM_015106493.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5988bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-16
    Organism Ovis aries musimon(Ovis orientalis musimon)
    Product sperm flagellar protein 2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942355.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    ATGCCAACTG TACAGCCCCT GATGAGCACA AAAGTTCAAA ACATGAAAAG CGAGGCTTTC 
    CGAGATCAAC GCTTGAGCAA AAGACGACAA AATGAAATCA TGGCCAAAAT CCAAGCAGCT
    ATCATACAGA TTCCTAAACC CACATCAAAT CGTACTCTGA AAACATTTGA CGCCCAAAAA
    TTAATGAAAA AGAAAAAAGA GGCAGAGGAT GTGGCCAATG AAATTAAGAA GTTTGAAGCA
    TTAATAAAAA AGGATCTCCA GGCAAAAGAA AGTGCATCCA AGACTTCTCT AGATACAGCA
    GGCCAGACAA CCGCTGATTT GTTAAACACT TATTCAGATG ATGACTACAT TAAAAAAATC
    CAAAAACGCC TTGAAGAAGA TGCTTTTGCA CGAGAGCAAA GGGAGAAAAG ACGGCGGAGA
    CTCTTAATGG ATCAGTTAAT AGCTCATGAA GCCCAAGAGG AGGCTTATCG AGAGGAGCAG
    TTGATCAACC GGCTGATGCG ACAGTCCCAG CAGGAACGCA GGATCGCTGT GCAGCTCATG
    CACGTTAGGC ACGAAAAGGA AGTTTTATGG CAAAACAGAA TTTTCAGAGA AAAACAATAT
    GAGGAAAGAC GGCTTAAAGA CTTCCAGGAT GCTCTTGATC GAGAAGCGGC TTTAGCCAGA
    CAAGCCAAGA TTGATTTTGA AGAACAAGCC CTCAGAGATA AGGAAATTCA TGAGCAGATC
    GCCATAGAAA GAGCTCAAGC TCGTTACAAA AAGCATTATT CAATATGTGC AGAAATTTTG
    GATCAGATAC TTGATCTGTC CACTAAGGTG GCAGACTATC GATTGTTGAC AAATAACCTG
    ATCCCATATA AGTTGATGCA TGATTGGAAG GAACTGTTTT TCCATGGAAA ACCCATATAT
    GAGCAAGCCT CTCTTAGACA TTTACCAGCT AAGCTCTCTG CAGAACACCT TGTAGAAATG
    GAGAAAAGGA ACTTGCTAGA TAACAATGAT TATGAAGAAT ATAAGAACAT GGTTGGAGAA
    TGGGCCTTAC CAGAAGAAAT GGTTGAAAAC TTACCACCCT CCAACAATTG CATATTGGGC
    CATGTGATTC ACAGACTAAT TGAAAGTTCT CTTCCTCCTC AAGTTGAATC TACAGTACCT
    GAGTTACCTT CATTTGCTAT AAAAGGATGC TTGTTGGGGA AAACCTTTAG TGGAAAAACT
    ACTGCTCTAA AGGCTCTAGA GAAAAGCTTT CCTATTCAGG TACTTTCTAT TGACACTCTT
    GTTCAAGAAG CTATCCAAGC ATTTCATGAC AATGAAAGTA TCAGCGGGGC CCATGGACTT
    CAGAAAGCAC CTGAAGAAAA GGTTTTACCA GTTAGGCGAA AGAGTGGTAA AGAAAACCAG
    GACGTGCAAG ATGAAGCACT ACCAGAAAGA GAAGATAAAA CCAGACTTGA TACCGATGCT
    AGAAAAGCAG CAAAAGCTGA AGAAGTCGTA GCGAGTGATA GTTTCTCTGA ACTCACTGTG
    CGTGCTCAGC TTGGTGCAAA ATCAGAAATG CTGCTGAAGA AAGGAAAGAG CATATCTGAT
    ATATTGCTAG TCAGCATCAT GGTAAATGCC ATTAATCAGA TACCTATGTA TAAAGGCTGG
    ATCCTGGATG GCTTTCCAAT GACATTAAAC CAAGCAAAAC TTCTGGAGGA AGCACTCACA
    GGCTACAACA GAAACCTCAT AGAACTGGAG GGAAAGAAAT CACAAACATC CACATTAGCT
    GTTGACCCTA CAGCTTCCAA AGAAGTGCCT CTTCCCCCTT CTGCATTTGA TTTTGTCATG
    TTATTAGACA TTTCAGATAA TTCCTCACTG AATCGCATGA ATGACATCAT GGCTAAAGCA
    TTTTCAAGTG AAGCTCCTCG TGAAGATATC AATCAGCGTG TAGCTGCTAA AAACAAAGAT
    ACAGATGAGG ACCAGAATTT AAGAGATCAA ATACAGCACA GAATTGTAGG CTTCCTGGAC
    AACTGGCCTT CGTTGGAGCA GTGGTTTACA GAGCCAGAAA ACATTTTGAT AAAAATCAAT
    GCTGAAGTAG ATAAACAGTC ATTGTTCCAA AAAGTAAAGG AAATGATTAT GACTGAAATA
    ATTAAAAAAG AAAATAAAGT GAAAAAGAAA TTAGAAGAAA AGGAAGCTGA GAGAAAAGAA
    GAAGTTTCCC TGAGTGAGAA GACATCCCCT CCTCCTCCTC CCCCTCCGGA ACCAGAGAAA
    GAGAAGGAAG TCCACCTTCC ACAGGAGCGG TCCAAAACTC CTGCTGCAAA AGGAAAGCAA
    GCATCAGAAC CCCTGCATGG TAATCGAGAA CCTCTTCAGG AAGGAAAAGG GAAGAAAAGT
    GAAACTTCAC TCAAAAGAAA AGGTTCTCCT AGAGGAAAAT CGCTAGGAGG GAAGATTCCA
    CTCAAGAAAT CACCTGCCGA GTCTACAGAT ATTTCACCGG TCCCGGTAGT GCCAGCCCCA
    TCGAAGCCCG GATCAGAAGA GTGGGTGTAT GTAAATGAAC CAATTCCTGA GGAAATACCA
    TCATTTTTAG TACCATACTG GAAATTGATA GAAAATTCCT ATATAAATTC CATCAAAACA
    GTACTCAGGG AGTTGAGAGA AGGCCAGCAT AGTGTACTTG CTTACTTATA TGATATTAGA
    ACAGGCTTCC AGCAGTTTCT GAAGCGTCCA GACCACAAGC AAGACTTCGT GTCTCAGTGG
    CAGGCGGATT TCAACTCCCT TCCTGATGAC CTGTGGGATG ATGAGGAAAC AAAGGCTGAG
    CTGCACCAAA GAGTGAATGA TTTACGGGAC CGCCTGTGGG ACATTTGCGA TGCCCGTAAG
    GAAGAAGCAG AGCAGGAGCG TCTGGACATC ATTAACGAGA GCTGGTTGCA GGACTCAATG
    GGAATTGCCA TGAACCATTT CTTCTCACTG ATGCAGGCAG AACTGAACCG TTTCCAAGAT
    ACGAAGAGAT TCCTGCAAGA TTATTACAGG GCCATGGAAT GTAAAATTCC GATGGATGAC
    ACTAAGAAAT TCACTCGAGT CCCACTGGTC CGACTGGATA GTAGAGATAT TTTGGAAAGC
    CAGCTTAGAA TTCCCCTGGT TCCTCGAAGA TCCATTTCTC CAGAGTCAGC TTTATCCAAA
    CCAAAAACAA AAAGCATCCT GAAGGGCAAG ATCGATTACA CGCTGGAAAG TGTAGAGTTA
    AACTTTGAGA CTGATGAGAA GATGGTGATG GATACGTGGC AGCAGTCTTC TTTGGCAGTA
    TCTCACATGG TGGCTGCTGA AATTCATCAG AGGCTCATGG AAGAAGAAAA AGAAAACCAG
    CCAGCAGAAA CAAAAGAAAA ACTTCCTCAG GCAGCTGCAA ATAAAAAAGT GAAAAAGGAG
    CCTCCGCCAC CGCCACCACC ACCCAAGAAA AAGAAAGAGG ACAAAAAAGG AAAAGGTAAA
    TCCCCACCTA TGGCAGAAGC TACTCCGGTA GTAGTGCCAG CAGAAGAGAC TGAGGAAGTG
    GAATTAAAAA ATGAACTGAA GCTCAGAATA AAGGAAGAGC ATCTTGCTGC CCTGCAATTT
    GAAGAGATAG CTACACAGTT CCGACTTGAA CTGATAAAGG TAAAAGCATT GGCTTTCCTG
    GAAGAGTTAG TAATAAAGGC GGTTGATGTA TATAGACTCA TGGACAAGTG GCTCGGTGAG
    AGGTACTTGA ATGAAATGGC CAGTGTGGAA AAACTAACTG GGGTAGCTCG CTATCACATT
    GAAACATCAA CCAAAATTCA GAATGAACTT TATTTAGACC AAGAAGACTT CTTCATTAAT
    GGCGATATCA AAGTCTTTCC AGATCCACCT CCTCCGGTAC GCCCTCCGCC TGTGGAAAAG
    GAGGAAAATG GCACCCTGAC CATGGAGCAG CTTGACAACC TCCGGGATCA GTTCTTAGAT
    ATAGCGCCCA AAGGCAGGGA TCCTCCAGAC AATGTGAGTG GATTCACCAA GAAGCAGAAA
    AGGCCTCGGA AGCTTGACCA TTACCTCCCA AAGAGGAACA ATCAATTTTT GAAAATTTGG
    CTTAAAAAAT ACCCTTGGCT TGTATACGAT GAGATACTAA ATCTTATGTT CTGCGGCCTG
    TGTCGTAAAC ATGGAGTGAC ATTAAAGGGA AATCAGGTCA GTTTTTTTTA TGGCACGGAT
    AACTTCAGGA CTGAATTTCT CAACGCGCAT CATCTGAGTG AGGCTCACGC CAAGGCGTCC
    CTCATGGAAG CCACGAGTGG TTCTCCAGGG AGCAGAGCTA CCACGGAGCT GATGGTGAGG
    ACCATGAGCA AGGTGACCCT TGGCAGGGTG GAGAACCTGT TCCGGTCCTG CCACGCCATC
    GCCAAGACCG GACGTCCCCT CAAAGACTTC CTCTGGATGT GTTCGCTGGA CGATATGAAG
    GGAGTTGATA TCGGCCCGGT GTTCAGAACC AGTAAATCGG CCAGAACTTT TACCTACTTT
    ATTGCTGAAG TGGAACGGAG AACTCTAAGA GAGAAGTTAG AAAAAAGTCA GTTCTTCTCC
    GTCCTCAGTG ATGGGATTGC AGACAGTTCA GCTGAGGAAG CTGAACTCGT ATATGTGCAG
    TTTGCCCGCG CAGGGAGAGT GCACTGCCAA ATCGTCGGGG TACAGACCGT GGAGAAGAAG
    GAGCCCTGGG CAATAAAACG TGCCATCGAG AAAACTCTGG AGAGAAATCT TCAACTTCGA
    CTGTCGAACC AAGACTGGGC AAAGAAACTG GTGGGTTTCG GAAGCGATGG AGCTCCAGGT
    TCAGAGGGAG AAAACGGCAG CACAGTGGCC CTGCTTTTAA GGGAAATCCA GCCAGGTGTG
    CTGCCTGTTT ACTGCCTTGC CCATCACCTC GAACTGTCTT ACCAGGCGGT GTTCCAGAGC
    GTTCCCCTCT ACAGCCGTGT GAAAGACCTT CTTCATAGCA TGCACCGCTT CTATCACGAT
    TCTCCTGCTC ACAGGAGTGC TCTGGTGACT GCTTTCAAGG GCCTCCACCT TCAACCTGCG
    ATGCCCTCTC AAGTCAGAGG CCGGAGGTGG CTTCAGGGAT TGCAGGCTGC CCTCCAGAAC
    TTTCTCAAGG GATATCCAGC AATTGTCCAG CAGCTGCAGT CAACAGAAGA AAGCAGAAGC
    AACAGCAGTC ATCAGAAAGC CGAAGAACTC CTGGAGTCTC TCCTCCAGGC AGACATTGTC
    AAATTTGTCC ACTTCTTGGT GGATGTCGTC AATGTCCTTA GCATACTGTC CCATGTCAAT
    CAGAACAGAA ATTCCTCAAT TGCTGATGTA TTTGCCACCT TAGAGTCAAC ACTGGAAATG
    CTCCAAATTT ATCAGACAAG ACCAGGGCCG AAAGAACGTG GGGTAGATTC GGTCACACAC
    TTTCATGGTA ACTGCCTTGG AGGAAAGGGA AACATTTCTG ACGTGAGAAA CATTGTTTTA
    ACACATCTTA TCAAGAGGCT GCGAGGCTGT TTCAGAGATG CCAACCAAGA TGTGGTGAAG
    GCAACCACGG TTGGAAGCTT TAAACTGTGG CCGGCAAAAA TAAATCAAGA ATTTGGAGAA
    AAAGAGGTGG CCATCCTGAT TGCACATTAT GAACCAGTAT TAGAGGCAGC CAAGGTGAAA
    ATTGACCAAG TAGACACAGA ATGGAGCATG TTGAAATTGG AGATCTACTC TAGATTTCAG
    AACATTTGCA AACTGACCTG GGATTTTGTG AATTCTGTTT ATTTACACAA GTATCCAAAC
    ATCCTGACAC TCATTGACCT TGTGCTGAGC CTGCCTGCAA GTTCAGCTGA GGCAGGACGT
    GGCTTTAGTC AGATGAAGCG CATCAGGTCC CAAATGCATG CCAAGGTCAA GGCTGACAAC
    ATGACGGATC TCCTGATAAT TCAGTTGAAT TCTCCAGACA TTAACAACTT TGACCCTAGG
    AAAGCCATTC ATTTATGGAA TACAAGAACA CCATCCTCAA CTGAGGACAC AAGACCTAAC
    TCAGACTGCT CATCAAACTC AGAAAGCCTT AGTGAAAGCG ATAAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_014961979.1
    CDS739..6726
    Translation

    Target ORF information:

    RefSeq Version XM_015106493.1
    Organism Ovis aries musimon(Ovis orientalis musimon)
    Definition Ovis aries musimon sperm flagellar 2 (SPEF2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015106493.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    ATGCCAACTG TACAGCCCCT GATGAGCACA AAAGTTCAAA ACATGAAAAG CGAGGCTTTC 
    CGAGATCAAC GCTTGAGCAA AAGACGACAA AATGAAATCA TGGCCAAAAT CCAAGCAGCT
    ATCATACAGA TTCCTAAACC CACATCAAAT CGTACTCTGA AAACATTTGA CGCCCAAAAA
    TTAATGAAAA AGAAAAAAGA GGCAGAGGAT GTGGCCAATG AAATTAAGAA GTTTGAAGCA
    TTAATAAAAA AGGATCTCCA GGCAAAAGAA AGTGCATCCA AGACTTCTCT AGATACAGCA
    GGCCAGACAA CCGCTGATTT GTTAAACACT TATTCAGATG ATGACTACAT TAAAAAAATC
    CAAAAACGCC TTGAAGAAGA TGCTTTTGCA CGAGAGCAAA GGGAGAAAAG ACGGCGGAGA
    CTCTTAATGG ATCAGTTAAT AGCTCATGAA GCCCAAGAGG AGGCTTATCG AGAGGAGCAG
    TTGATCAACC GGCTGATGCG ACAGTCCCAG CAGGAACGCA GGATCGCTGT GCAGCTCATG
    CACGTTAGGC ACGAAAAGGA AGTTTTATGG CAAAACAGAA TTTTCAGAGA AAAACAATAT
    GAGGAAAGAC GGCTTAAAGA CTTCCAGGAT GCTCTTGATC GAGAAGCGGC TTTAGCCAGA
    CAAGCCAAGA TTGATTTTGA AGAACAAGCC CTCAGAGATA AGGAAATTCA TGAGCAGATC
    GCCATAGAAA GAGCTCAAGC TCGTTACAAA AAGCATTATT CAATATGTGC AGAAATTTTG
    GATCAGATAC TTGATCTGTC CACTAAGGTG GCAGACTATC GATTGTTGAC AAATAACCTG
    ATCCCATATA AGTTGATGCA TGATTGGAAG GAACTGTTTT TCCATGGAAA ACCCATATAT
    GAGCAAGCCT CTCTTAGACA TTTACCAGCT AAGCTCTCTG CAGAACACCT TGTAGAAATG
    GAGAAAAGGA ACTTGCTAGA TAACAATGAT TATGAAGAAT ATAAGAACAT GGTTGGAGAA
    TGGGCCTTAC CAGAAGAAAT GGTTGAAAAC TTACCACCCT CCAACAATTG CATATTGGGC
    CATGTGATTC ACAGACTAAT TGAAAGTTCT CTTCCTCCTC AAGTTGAATC TACAGTACCT
    GAGTTACCTT CATTTGCTAT AAAAGGATGC TTGTTGGGGA AAACCTTTAG TGGAAAAACT
    ACTGCTCTAA AGGCTCTAGA GAAAAGCTTT CCTATTCAGG TACTTTCTAT TGACACTCTT
    GTTCAAGAAG CTATCCAAGC ATTTCATGAC AATGAAAGTA TCAGCGGGGC CCATGGACTT
    CAGAAAGCAC CTGAAGAAAA GGTTTTACCA GTTAGGCGAA AGAGTGGTAA AGAAAACCAG
    GACGTGCAAG ATGAAGCACT ACCAGAAAGA GAAGATAAAA CCAGACTTGA TACCGATGCT
    AGAAAAGCAG CAAAAGCTGA AGAAGTCGTA GCGAGTGATA GTTTCTCTGA ACTCACTGTG
    CGTGCTCAGC TTGGTGCAAA ATCAGAAATG CTGCTGAAGA AAGGAAAGAG CATATCTGAT
    ATATTGCTAG TCAGCATCAT GGTAAATGCC ATTAATCAGA TACCTATGTA TAAAGGCTGG
    ATCCTGGATG GCTTTCCAAT GACATTAAAC CAAGCAAAAC TTCTGGAGGA AGCACTCACA
    GGCTACAACA GAAACCTCAT AGAACTGGAG GGAAAGAAAT CACAAACATC CACATTAGCT
    GTTGACCCTA CAGCTTCCAA AGAAGTGCCT CTTCCCCCTT CTGCATTTGA TTTTGTCATG
    TTATTAGACA TTTCAGATAA TTCCTCACTG AATCGCATGA ATGACATCAT GGCTAAAGCA
    TTTTCAAGTG AAGCTCCTCG TGAAGATATC AATCAGCGTG TAGCTGCTAA AAACAAAGAT
    ACAGATGAGG ACCAGAATTT AAGAGATCAA ATACAGCACA GAATTGTAGG CTTCCTGGAC
    AACTGGCCTT CGTTGGAGCA GTGGTTTACA GAGCCAGAAA ACATTTTGAT AAAAATCAAT
    GCTGAAGTAG ATAAACAGTC ATTGTTCCAA AAAGTAAAGG AAATGATTAT GACTGAAATA
    ATTAAAAAAG AAAATAAAGT GAAAAAGAAA TTAGAAGAAA AGGAAGCTGA GAGAAAAGAA
    GAAGTTTCCC TGAGTGAGAA GACATCCCCT CCTCCTCCTC CCCCTCCGGA ACCAGAGAAA
    GAGAAGGAAG TCCACCTTCC ACAGGAGCGG TCCAAAACTC CTGCTGCAAA AGGAAAGCAA
    GCATCAGAAC CCCTGCATGG TAATCGAGAA CCTCTTCAGG AAGGAAAAGG GAAGAAAAGT
    GAAACTTCAC TCAAAAGAAA AGGTTCTCCT AGAGGAAAAT CGCTAGGAGG GAAGATTCCA
    CTCAAGAAAT CACCTGCCGA GTCTACAGAT ATTTCACCGG TCCCGGTAGT GCCAGCCCCA
    TCGAAGCCCG GATCAGAAGA GTGGGTGTAT GTAAATGAAC CAATTCCTGA GGAAATACCA
    TCATTTTTAG TACCATACTG GAAATTGATA GAAAATTCCT ATATAAATTC CATCAAAACA
    GTACTCAGGG AGTTGAGAGA AGGCCAGCAT AGTGTACTTG CTTACTTATA TGATATTAGA
    ACAGGCTTCC AGCAGTTTCT GAAGCGTCCA GACCACAAGC AAGACTTCGT GTCTCAGTGG
    CAGGCGGATT TCAACTCCCT TCCTGATGAC CTGTGGGATG ATGAGGAAAC AAAGGCTGAG
    CTGCACCAAA GAGTGAATGA TTTACGGGAC CGCCTGTGGG ACATTTGCGA TGCCCGTAAG
    GAAGAAGCAG AGCAGGAGCG TCTGGACATC ATTAACGAGA GCTGGTTGCA GGACTCAATG
    GGAATTGCCA TGAACCATTT CTTCTCACTG ATGCAGGCAG AACTGAACCG TTTCCAAGAT
    ACGAAGAGAT TCCTGCAAGA TTATTACAGG GCCATGGAAT GTAAAATTCC GATGGATGAC
    ACTAAGAAAT TCACTCGAGT CCCACTGGTC CGACTGGATA GTAGAGATAT TTTGGAAAGC
    CAGCTTAGAA TTCCCCTGGT TCCTCGAAGA TCCATTTCTC CAGAGTCAGC TTTATCCAAA
    CCAAAAACAA AAAGCATCCT GAAGGGCAAG ATCGATTACA CGCTGGAAAG TGTAGAGTTA
    AACTTTGAGA CTGATGAGAA GATGGTGATG GATACGTGGC AGCAGTCTTC TTTGGCAGTA
    TCTCACATGG TGGCTGCTGA AATTCATCAG AGGCTCATGG AAGAAGAAAA AGAAAACCAG
    CCAGCAGAAA CAAAAGAAAA ACTTCCTCAG GCAGCTGCAA ATAAAAAAGT GAAAAAGGAG
    CCTCCGCCAC CGCCACCACC ACCCAAGAAA AAGAAAGAGG ACAAAAAAGG AAAAGGTAAA
    TCCCCACCTA TGGCAGAAGC TACTCCGGTA GTAGTGCCAG CAGAAGAGAC TGAGGAAGTG
    GAATTAAAAA ATGAACTGAA GCTCAGAATA AAGGAAGAGC ATCTTGCTGC CCTGCAATTT
    GAAGAGATAG CTACACAGTT CCGACTTGAA CTGATAAAGG TAAAAGCATT GGCTTTCCTG
    GAAGAGTTAG TAATAAAGGC GGTTGATGTA TATAGACTCA TGGACAAGTG GCTCGGTGAG
    AGGTACTTGA ATGAAATGGC CAGTGTGGAA AAACTAACTG GGGTAGCTCG CTATCACATT
    GAAACATCAA CCAAAATTCA GAATGAACTT TATTTAGACC AAGAAGACTT CTTCATTAAT
    GGCGATATCA AAGTCTTTCC AGATCCACCT CCTCCGGTAC GCCCTCCGCC TGTGGAAAAG
    GAGGAAAATG GCACCCTGAC CATGGAGCAG CTTGACAACC TCCGGGATCA GTTCTTAGAT
    ATAGCGCCCA AAGGCAGGGA TCCTCCAGAC AATGTGAGTG GATTCACCAA GAAGCAGAAA
    AGGCCTCGGA AGCTTGACCA TTACCTCCCA AAGAGGAACA ATCAATTTTT GAAAATTTGG
    CTTAAAAAAT ACCCTTGGCT TGTATACGAT GAGATACTAA ATCTTATGTT CTGCGGCCTG
    TGTCGTAAAC ATGGAGTGAC ATTAAAGGGA AATCAGGTCA GTTTTTTTTA TGGCACGGAT
    AACTTCAGGA CTGAATTTCT CAACGCGCAT CATCTGAGTG AGGCTCACGC CAAGGCGTCC
    CTCATGGAAG CCACGAGTGG TTCTCCAGGG AGCAGAGCTA CCACGGAGCT GATGGTGAGG
    ACCATGAGCA AGGTGACCCT TGGCAGGGTG GAGAACCTGT TCCGGTCCTG CCACGCCATC
    GCCAAGACCG GACGTCCCCT CAAAGACTTC CTCTGGATGT GTTCGCTGGA CGATATGAAG
    GGAGTTGATA TCGGCCCGGT GTTCAGAACC AGTAAATCGG CCAGAACTTT TACCTACTTT
    ATTGCTGAAG TGGAACGGAG AACTCTAAGA GAGAAGTTAG AAAAAAGTCA GTTCTTCTCC
    GTCCTCAGTG ATGGGATTGC AGACAGTTCA GCTGAGGAAG CTGAACTCGT ATATGTGCAG
    TTTGCCCGCG CAGGGAGAGT GCACTGCCAA ATCGTCGGGG TACAGACCGT GGAGAAGAAG
    GAGCCCTGGG CAATAAAACG TGCCATCGAG AAAACTCTGG AGAGAAATCT TCAACTTCGA
    CTGTCGAACC AAGACTGGGC AAAGAAACTG GTGGGTTTCG GAAGCGATGG AGCTCCAGGT
    TCAGAGGGAG AAAACGGCAG CACAGTGGCC CTGCTTTTAA GGGAAATCCA GCCAGGTGTG
    CTGCCTGTTT ACTGCCTTGC CCATCACCTC GAACTGTCTT ACCAGGCGGT GTTCCAGAGC
    GTTCCCCTCT ACAGCCGTGT GAAAGACCTT CTTCATAGCA TGCACCGCTT CTATCACGAT
    TCTCCTGCTC ACAGGAGTGC TCTGGTGACT GCTTTCAAGG GCCTCCACCT TCAACCTGCG
    ATGCCCTCTC AAGTCAGAGG CCGGAGGTGG CTTCAGGGAT TGCAGGCTGC CCTCCAGAAC
    TTTCTCAAGG GATATCCAGC AATTGTCCAG CAGCTGCAGT CAACAGAAGA AAGCAGAAGC
    AACAGCAGTC ATCAGAAAGC CGAAGAACTC CTGGAGTCTC TCCTCCAGGC AGACATTGTC
    AAATTTGTCC ACTTCTTGGT GGATGTCGTC AATGTCCTTA GCATACTGTC CCATGTCAAT
    CAGAACAGAA ATTCCTCAAT TGCTGATGTA TTTGCCACCT TAGAGTCAAC ACTGGAAATG
    CTCCAAATTT ATCAGACAAG ACCAGGGCCG AAAGAACGTG GGGTAGATTC GGTCACACAC
    TTTCATGGTA ACTGCCTTGG AGGAAAGGGA AACATTTCTG ACGTGAGAAA CATTGTTTTA
    ACACATCTTA TCAAGAGGCT GCGAGGCTGT TTCAGAGATG CCAACCAAGA TGTGGTGAAG
    GCAACCACGG TTGGAAGCTT TAAACTGTGG CCGGCAAAAA TAAATCAAGA ATTTGGAGAA
    AAAGAGGTGG CCATCCTGAT TGCACATTAT GAACCAGTAT TAGAGGCAGC CAAGGTGAAA
    ATTGACCAAG TAGACACAGA ATGGAGCATG TTGAAATTGG AGATCTACTC TAGATTTCAG
    AACATTTGCA AACTGACCTG GGATTTTGTG AATTCTGTTT ATTTACACAA GTATCCAAAC
    ATCCTGACAC TCATTGACCT TGTGCTGAGC CTGCCTGCAA GTTCAGCTGA GGCAGGACGT
    GGCTTTAGTC AGATGAAGCG CATCAGGTCC CAAATGCATG CCAAGGTCAA GGCTGACAAC
    ATGACGGATC TCCTGATAAT TCAGTTGAAT TCTCCAGACA TTAACAACTT TGACCCTAGG
    AAAGCCATTC ATTTATGGAA TACAAGAACA CCATCCTCAA CTGAGGACAC AAGACCTAAC
    TCAGACTGCT CATCAAACTC AGAAAGCCTT AGTGAAAGCG ATAAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OOv13487
    Clone ID Related Accession (Same CDS sequence) XM_015106493.1 , XM_015106492.1
    Accession Version XM_015106492.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5988bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-16
    Organism Ovis aries musimon(Ovis orientalis musimon)
    Product sperm flagellar protein 2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942355.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    ATGCCAACTG TACAGCCCCT GATGAGCACA AAAGTTCAAA ACATGAAAAG CGAGGCTTTC 
    CGAGATCAAC GCTTGAGCAA AAGACGACAA AATGAAATCA TGGCCAAAAT CCAAGCAGCT
    ATCATACAGA TTCCTAAACC CACATCAAAT CGTACTCTGA AAACATTTGA CGCCCAAAAA
    TTAATGAAAA AGAAAAAAGA GGCAGAGGAT GTGGCCAATG AAATTAAGAA GTTTGAAGCA
    TTAATAAAAA AGGATCTCCA GGCAAAAGAA AGTGCATCCA AGACTTCTCT AGATACAGCA
    GGCCAGACAA CCGCTGATTT GTTAAACACT TATTCAGATG ATGACTACAT TAAAAAAATC
    CAAAAACGCC TTGAAGAAGA TGCTTTTGCA CGAGAGCAAA GGGAGAAAAG ACGGCGGAGA
    CTCTTAATGG ATCAGTTAAT AGCTCATGAA GCCCAAGAGG AGGCTTATCG AGAGGAGCAG
    TTGATCAACC GGCTGATGCG ACAGTCCCAG CAGGAACGCA GGATCGCTGT GCAGCTCATG
    CACGTTAGGC ACGAAAAGGA AGTTTTATGG CAAAACAGAA TTTTCAGAGA AAAACAATAT
    GAGGAAAGAC GGCTTAAAGA CTTCCAGGAT GCTCTTGATC GAGAAGCGGC TTTAGCCAGA
    CAAGCCAAGA TTGATTTTGA AGAACAAGCC CTCAGAGATA AGGAAATTCA TGAGCAGATC
    GCCATAGAAA GAGCTCAAGC TCGTTACAAA AAGCATTATT CAATATGTGC AGAAATTTTG
    GATCAGATAC TTGATCTGTC CACTAAGGTG GCAGACTATC GATTGTTGAC AAATAACCTG
    ATCCCATATA AGTTGATGCA TGATTGGAAG GAACTGTTTT TCCATGGAAA ACCCATATAT
    GAGCAAGCCT CTCTTAGACA TTTACCAGCT AAGCTCTCTG CAGAACACCT TGTAGAAATG
    GAGAAAAGGA ACTTGCTAGA TAACAATGAT TATGAAGAAT ATAAGAACAT GGTTGGAGAA
    TGGGCCTTAC CAGAAGAAAT GGTTGAAAAC TTACCACCCT CCAACAATTG CATATTGGGC
    CATGTGATTC ACAGACTAAT TGAAAGTTCT CTTCCTCCTC AAGTTGAATC TACAGTACCT
    GAGTTACCTT CATTTGCTAT AAAAGGATGC TTGTTGGGGA AAACCTTTAG TGGAAAAACT
    ACTGCTCTAA AGGCTCTAGA GAAAAGCTTT CCTATTCAGG TACTTTCTAT TGACACTCTT
    GTTCAAGAAG CTATCCAAGC ATTTCATGAC AATGAAAGTA TCAGCGGGGC CCATGGACTT
    CAGAAAGCAC CTGAAGAAAA GGTTTTACCA GTTAGGCGAA AGAGTGGTAA AGAAAACCAG
    GACGTGCAAG ATGAAGCACT ACCAGAAAGA GAAGATAAAA CCAGACTTGA TACCGATGCT
    AGAAAAGCAG CAAAAGCTGA AGAAGTCGTA GCGAGTGATA GTTTCTCTGA ACTCACTGTG
    CGTGCTCAGC TTGGTGCAAA ATCAGAAATG CTGCTGAAGA AAGGAAAGAG CATATCTGAT
    ATATTGCTAG TCAGCATCAT GGTAAATGCC ATTAATCAGA TACCTATGTA TAAAGGCTGG
    ATCCTGGATG GCTTTCCAAT GACATTAAAC CAAGCAAAAC TTCTGGAGGA AGCACTCACA
    GGCTACAACA GAAACCTCAT AGAACTGGAG GGAAAGAAAT CACAAACATC CACATTAGCT
    GTTGACCCTA CAGCTTCCAA AGAAGTGCCT CTTCCCCCTT CTGCATTTGA TTTTGTCATG
    TTATTAGACA TTTCAGATAA TTCCTCACTG AATCGCATGA ATGACATCAT GGCTAAAGCA
    TTTTCAAGTG AAGCTCCTCG TGAAGATATC AATCAGCGTG TAGCTGCTAA AAACAAAGAT
    ACAGATGAGG ACCAGAATTT AAGAGATCAA ATACAGCACA GAATTGTAGG CTTCCTGGAC
    AACTGGCCTT CGTTGGAGCA GTGGTTTACA GAGCCAGAAA ACATTTTGAT AAAAATCAAT
    GCTGAAGTAG ATAAACAGTC ATTGTTCCAA AAAGTAAAGG AAATGATTAT GACTGAAATA
    ATTAAAAAAG AAAATAAAGT GAAAAAGAAA TTAGAAGAAA AGGAAGCTGA GAGAAAAGAA
    GAAGTTTCCC TGAGTGAGAA GACATCCCCT CCTCCTCCTC CCCCTCCGGA ACCAGAGAAA
    GAGAAGGAAG TCCACCTTCC ACAGGAGCGG TCCAAAACTC CTGCTGCAAA AGGAAAGCAA
    GCATCAGAAC CCCTGCATGG TAATCGAGAA CCTCTTCAGG AAGGAAAAGG GAAGAAAAGT
    GAAACTTCAC TCAAAAGAAA AGGTTCTCCT AGAGGAAAAT CGCTAGGAGG GAAGATTCCA
    CTCAAGAAAT CACCTGCCGA GTCTACAGAT ATTTCACCGG TCCCGGTAGT GCCAGCCCCA
    TCGAAGCCCG GATCAGAAGA GTGGGTGTAT GTAAATGAAC CAATTCCTGA GGAAATACCA
    TCATTTTTAG TACCATACTG GAAATTGATA GAAAATTCCT ATATAAATTC CATCAAAACA
    GTACTCAGGG AGTTGAGAGA AGGCCAGCAT AGTGTACTTG CTTACTTATA TGATATTAGA
    ACAGGCTTCC AGCAGTTTCT GAAGCGTCCA GACCACAAGC AAGACTTCGT GTCTCAGTGG
    CAGGCGGATT TCAACTCCCT TCCTGATGAC CTGTGGGATG ATGAGGAAAC AAAGGCTGAG
    CTGCACCAAA GAGTGAATGA TTTACGGGAC CGCCTGTGGG ACATTTGCGA TGCCCGTAAG
    GAAGAAGCAG AGCAGGAGCG TCTGGACATC ATTAACGAGA GCTGGTTGCA GGACTCAATG
    GGAATTGCCA TGAACCATTT CTTCTCACTG ATGCAGGCAG AACTGAACCG TTTCCAAGAT
    ACGAAGAGAT TCCTGCAAGA TTATTACAGG GCCATGGAAT GTAAAATTCC GATGGATGAC
    ACTAAGAAAT TCACTCGAGT CCCACTGGTC CGACTGGATA GTAGAGATAT TTTGGAAAGC
    CAGCTTAGAA TTCCCCTGGT TCCTCGAAGA TCCATTTCTC CAGAGTCAGC TTTATCCAAA
    CCAAAAACAA AAAGCATCCT GAAGGGCAAG ATCGATTACA CGCTGGAAAG TGTAGAGTTA
    AACTTTGAGA CTGATGAGAA GATGGTGATG GATACGTGGC AGCAGTCTTC TTTGGCAGTA
    TCTCACATGG TGGCTGCTGA AATTCATCAG AGGCTCATGG AAGAAGAAAA AGAAAACCAG
    CCAGCAGAAA CAAAAGAAAA ACTTCCTCAG GCAGCTGCAA ATAAAAAAGT GAAAAAGGAG
    CCTCCGCCAC CGCCACCACC ACCCAAGAAA AAGAAAGAGG ACAAAAAAGG AAAAGGTAAA
    TCCCCACCTA TGGCAGAAGC TACTCCGGTA GTAGTGCCAG CAGAAGAGAC TGAGGAAGTG
    GAATTAAAAA ATGAACTGAA GCTCAGAATA AAGGAAGAGC ATCTTGCTGC CCTGCAATTT
    GAAGAGATAG CTACACAGTT CCGACTTGAA CTGATAAAGG TAAAAGCATT GGCTTTCCTG
    GAAGAGTTAG TAATAAAGGC GGTTGATGTA TATAGACTCA TGGACAAGTG GCTCGGTGAG
    AGGTACTTGA ATGAAATGGC CAGTGTGGAA AAACTAACTG GGGTAGCTCG CTATCACATT
    GAAACATCAA CCAAAATTCA GAATGAACTT TATTTAGACC AAGAAGACTT CTTCATTAAT
    GGCGATATCA AAGTCTTTCC AGATCCACCT CCTCCGGTAC GCCCTCCGCC TGTGGAAAAG
    GAGGAAAATG GCACCCTGAC CATGGAGCAG CTTGACAACC TCCGGGATCA GTTCTTAGAT
    ATAGCGCCCA AAGGCAGGGA TCCTCCAGAC AATGTGAGTG GATTCACCAA GAAGCAGAAA
    AGGCCTCGGA AGCTTGACCA TTACCTCCCA AAGAGGAACA ATCAATTTTT GAAAATTTGG
    CTTAAAAAAT ACCCTTGGCT TGTATACGAT GAGATACTAA ATCTTATGTT CTGCGGCCTG
    TGTCGTAAAC ATGGAGTGAC ATTAAAGGGA AATCAGGTCA GTTTTTTTTA TGGCACGGAT
    AACTTCAGGA CTGAATTTCT CAACGCGCAT CATCTGAGTG AGGCTCACGC CAAGGCGTCC
    CTCATGGAAG CCACGAGTGG TTCTCCAGGG AGCAGAGCTA CCACGGAGCT GATGGTGAGG
    ACCATGAGCA AGGTGACCCT TGGCAGGGTG GAGAACCTGT TCCGGTCCTG CCACGCCATC
    GCCAAGACCG GACGTCCCCT CAAAGACTTC CTCTGGATGT GTTCGCTGGA CGATATGAAG
    GGAGTTGATA TCGGCCCGGT GTTCAGAACC AGTAAATCGG CCAGAACTTT TACCTACTTT
    ATTGCTGAAG TGGAACGGAG AACTCTAAGA GAGAAGTTAG AAAAAAGTCA GTTCTTCTCC
    GTCCTCAGTG ATGGGATTGC AGACAGTTCA GCTGAGGAAG CTGAACTCGT ATATGTGCAG
    TTTGCCCGCG CAGGGAGAGT GCACTGCCAA ATCGTCGGGG TACAGACCGT GGAGAAGAAG
    GAGCCCTGGG CAATAAAACG TGCCATCGAG AAAACTCTGG AGAGAAATCT TCAACTTCGA
    CTGTCGAACC AAGACTGGGC AAAGAAACTG GTGGGTTTCG GAAGCGATGG AGCTCCAGGT
    TCAGAGGGAG AAAACGGCAG CACAGTGGCC CTGCTTTTAA GGGAAATCCA GCCAGGTGTG
    CTGCCTGTTT ACTGCCTTGC CCATCACCTC GAACTGTCTT ACCAGGCGGT GTTCCAGAGC
    GTTCCCCTCT ACAGCCGTGT GAAAGACCTT CTTCATAGCA TGCACCGCTT CTATCACGAT
    TCTCCTGCTC ACAGGAGTGC TCTGGTGACT GCTTTCAAGG GCCTCCACCT TCAACCTGCG
    ATGCCCTCTC AAGTCAGAGG CCGGAGGTGG CTTCAGGGAT TGCAGGCTGC CCTCCAGAAC
    TTTCTCAAGG GATATCCAGC AATTGTCCAG CAGCTGCAGT CAACAGAAGA AAGCAGAAGC
    AACAGCAGTC ATCAGAAAGC CGAAGAACTC CTGGAGTCTC TCCTCCAGGC AGACATTGTC
    AAATTTGTCC ACTTCTTGGT GGATGTCGTC AATGTCCTTA GCATACTGTC CCATGTCAAT
    CAGAACAGAA ATTCCTCAAT TGCTGATGTA TTTGCCACCT TAGAGTCAAC ACTGGAAATG
    CTCCAAATTT ATCAGACAAG ACCAGGGCCG AAAGAACGTG GGGTAGATTC GGTCACACAC
    TTTCATGGTA ACTGCCTTGG AGGAAAGGGA AACATTTCTG ACGTGAGAAA CATTGTTTTA
    ACACATCTTA TCAAGAGGCT GCGAGGCTGT TTCAGAGATG CCAACCAAGA TGTGGTGAAG
    GCAACCACGG TTGGAAGCTT TAAACTGTGG CCGGCAAAAA TAAATCAAGA ATTTGGAGAA
    AAAGAGGTGG CCATCCTGAT TGCACATTAT GAACCAGTAT TAGAGGCAGC CAAGGTGAAA
    ATTGACCAAG TAGACACAGA ATGGAGCATG TTGAAATTGG AGATCTACTC TAGATTTCAG
    AACATTTGCA AACTGACCTG GGATTTTGTG AATTCTGTTT ATTTACACAA GTATCCAAAC
    ATCCTGACAC TCATTGACCT TGTGCTGAGC CTGCCTGCAA GTTCAGCTGA GGCAGGACGT
    GGCTTTAGTC AGATGAAGCG CATCAGGTCC CAAATGCATG CCAAGGTCAA GGCTGACAAC
    ATGACGGATC TCCTGATAAT TCAGTTGAAT TCTCCAGACA TTAACAACTT TGACCCTAGG
    AAAGCCATTC ATTTATGGAA TACAAGAACA CCATCCTCAA CTGAGGACAC AAGACCTAAC
    TCAGACTGCT CATCAAACTC AGAAAGCCTT AGTGAAAGCG ATAAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_014961978.1
    CDS743..6730
    Translation

    Target ORF information:

    RefSeq Version XM_015106492.1
    Organism Ovis aries musimon(Ovis orientalis musimon)
    Definition Ovis aries musimon sperm flagellar 2 (SPEF2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015106492.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    ATGCCAACTG TACAGCCCCT GATGAGCACA AAAGTTCAAA ACATGAAAAG CGAGGCTTTC 
    CGAGATCAAC GCTTGAGCAA AAGACGACAA AATGAAATCA TGGCCAAAAT CCAAGCAGCT
    ATCATACAGA TTCCTAAACC CACATCAAAT CGTACTCTGA AAACATTTGA CGCCCAAAAA
    TTAATGAAAA AGAAAAAAGA GGCAGAGGAT GTGGCCAATG AAATTAAGAA GTTTGAAGCA
    TTAATAAAAA AGGATCTCCA GGCAAAAGAA AGTGCATCCA AGACTTCTCT AGATACAGCA
    GGCCAGACAA CCGCTGATTT GTTAAACACT TATTCAGATG ATGACTACAT TAAAAAAATC
    CAAAAACGCC TTGAAGAAGA TGCTTTTGCA CGAGAGCAAA GGGAGAAAAG ACGGCGGAGA
    CTCTTAATGG ATCAGTTAAT AGCTCATGAA GCCCAAGAGG AGGCTTATCG AGAGGAGCAG
    TTGATCAACC GGCTGATGCG ACAGTCCCAG CAGGAACGCA GGATCGCTGT GCAGCTCATG
    CACGTTAGGC ACGAAAAGGA AGTTTTATGG CAAAACAGAA TTTTCAGAGA AAAACAATAT
    GAGGAAAGAC GGCTTAAAGA CTTCCAGGAT GCTCTTGATC GAGAAGCGGC TTTAGCCAGA
    CAAGCCAAGA TTGATTTTGA AGAACAAGCC CTCAGAGATA AGGAAATTCA TGAGCAGATC
    GCCATAGAAA GAGCTCAAGC TCGTTACAAA AAGCATTATT CAATATGTGC AGAAATTTTG
    GATCAGATAC TTGATCTGTC CACTAAGGTG GCAGACTATC GATTGTTGAC AAATAACCTG
    ATCCCATATA AGTTGATGCA TGATTGGAAG GAACTGTTTT TCCATGGAAA ACCCATATAT
    GAGCAAGCCT CTCTTAGACA TTTACCAGCT AAGCTCTCTG CAGAACACCT TGTAGAAATG
    GAGAAAAGGA ACTTGCTAGA TAACAATGAT TATGAAGAAT ATAAGAACAT GGTTGGAGAA
    TGGGCCTTAC CAGAAGAAAT GGTTGAAAAC TTACCACCCT CCAACAATTG CATATTGGGC
    CATGTGATTC ACAGACTAAT TGAAAGTTCT CTTCCTCCTC AAGTTGAATC TACAGTACCT
    GAGTTACCTT CATTTGCTAT AAAAGGATGC TTGTTGGGGA AAACCTTTAG TGGAAAAACT
    ACTGCTCTAA AGGCTCTAGA GAAAAGCTTT CCTATTCAGG TACTTTCTAT TGACACTCTT
    GTTCAAGAAG CTATCCAAGC ATTTCATGAC AATGAAAGTA TCAGCGGGGC CCATGGACTT
    CAGAAAGCAC CTGAAGAAAA GGTTTTACCA GTTAGGCGAA AGAGTGGTAA AGAAAACCAG
    GACGTGCAAG ATGAAGCACT ACCAGAAAGA GAAGATAAAA CCAGACTTGA TACCGATGCT
    AGAAAAGCAG CAAAAGCTGA AGAAGTCGTA GCGAGTGATA GTTTCTCTGA ACTCACTGTG
    CGTGCTCAGC TTGGTGCAAA ATCAGAAATG CTGCTGAAGA AAGGAAAGAG CATATCTGAT
    ATATTGCTAG TCAGCATCAT GGTAAATGCC ATTAATCAGA TACCTATGTA TAAAGGCTGG
    ATCCTGGATG GCTTTCCAAT GACATTAAAC CAAGCAAAAC TTCTGGAGGA AGCACTCACA
    GGCTACAACA GAAACCTCAT AGAACTGGAG GGAAAGAAAT CACAAACATC CACATTAGCT
    GTTGACCCTA CAGCTTCCAA AGAAGTGCCT CTTCCCCCTT CTGCATTTGA TTTTGTCATG
    TTATTAGACA TTTCAGATAA TTCCTCACTG AATCGCATGA ATGACATCAT GGCTAAAGCA
    TTTTCAAGTG AAGCTCCTCG TGAAGATATC AATCAGCGTG TAGCTGCTAA AAACAAAGAT
    ACAGATGAGG ACCAGAATTT AAGAGATCAA ATACAGCACA GAATTGTAGG CTTCCTGGAC
    AACTGGCCTT CGTTGGAGCA GTGGTTTACA GAGCCAGAAA ACATTTTGAT AAAAATCAAT
    GCTGAAGTAG ATAAACAGTC ATTGTTCCAA AAAGTAAAGG AAATGATTAT GACTGAAATA
    ATTAAAAAAG AAAATAAAGT GAAAAAGAAA TTAGAAGAAA AGGAAGCTGA GAGAAAAGAA
    GAAGTTTCCC TGAGTGAGAA GACATCCCCT CCTCCTCCTC CCCCTCCGGA ACCAGAGAAA
    GAGAAGGAAG TCCACCTTCC ACAGGAGCGG TCCAAAACTC CTGCTGCAAA AGGAAAGCAA
    GCATCAGAAC CCCTGCATGG TAATCGAGAA CCTCTTCAGG AAGGAAAAGG GAAGAAAAGT
    GAAACTTCAC TCAAAAGAAA AGGTTCTCCT AGAGGAAAAT CGCTAGGAGG GAAGATTCCA
    CTCAAGAAAT CACCTGCCGA GTCTACAGAT ATTTCACCGG TCCCGGTAGT GCCAGCCCCA
    TCGAAGCCCG GATCAGAAGA GTGGGTGTAT GTAAATGAAC CAATTCCTGA GGAAATACCA
    TCATTTTTAG TACCATACTG GAAATTGATA GAAAATTCCT ATATAAATTC CATCAAAACA
    GTACTCAGGG AGTTGAGAGA AGGCCAGCAT AGTGTACTTG CTTACTTATA TGATATTAGA
    ACAGGCTTCC AGCAGTTTCT GAAGCGTCCA GACCACAAGC AAGACTTCGT GTCTCAGTGG
    CAGGCGGATT TCAACTCCCT TCCTGATGAC CTGTGGGATG ATGAGGAAAC AAAGGCTGAG
    CTGCACCAAA GAGTGAATGA TTTACGGGAC CGCCTGTGGG ACATTTGCGA TGCCCGTAAG
    GAAGAAGCAG AGCAGGAGCG TCTGGACATC ATTAACGAGA GCTGGTTGCA GGACTCAATG
    GGAATTGCCA TGAACCATTT CTTCTCACTG ATGCAGGCAG AACTGAACCG TTTCCAAGAT
    ACGAAGAGAT TCCTGCAAGA TTATTACAGG GCCATGGAAT GTAAAATTCC GATGGATGAC
    ACTAAGAAAT TCACTCGAGT CCCACTGGTC CGACTGGATA GTAGAGATAT TTTGGAAAGC
    CAGCTTAGAA TTCCCCTGGT TCCTCGAAGA TCCATTTCTC CAGAGTCAGC TTTATCCAAA
    CCAAAAACAA AAAGCATCCT GAAGGGCAAG ATCGATTACA CGCTGGAAAG TGTAGAGTTA
    AACTTTGAGA CTGATGAGAA GATGGTGATG GATACGTGGC AGCAGTCTTC TTTGGCAGTA
    TCTCACATGG TGGCTGCTGA AATTCATCAG AGGCTCATGG AAGAAGAAAA AGAAAACCAG
    CCAGCAGAAA CAAAAGAAAA ACTTCCTCAG GCAGCTGCAA ATAAAAAAGT GAAAAAGGAG
    CCTCCGCCAC CGCCACCACC ACCCAAGAAA AAGAAAGAGG ACAAAAAAGG AAAAGGTAAA
    TCCCCACCTA TGGCAGAAGC TACTCCGGTA GTAGTGCCAG CAGAAGAGAC TGAGGAAGTG
    GAATTAAAAA ATGAACTGAA GCTCAGAATA AAGGAAGAGC ATCTTGCTGC CCTGCAATTT
    GAAGAGATAG CTACACAGTT CCGACTTGAA CTGATAAAGG TAAAAGCATT GGCTTTCCTG
    GAAGAGTTAG TAATAAAGGC GGTTGATGTA TATAGACTCA TGGACAAGTG GCTCGGTGAG
    AGGTACTTGA ATGAAATGGC CAGTGTGGAA AAACTAACTG GGGTAGCTCG CTATCACATT
    GAAACATCAA CCAAAATTCA GAATGAACTT TATTTAGACC AAGAAGACTT CTTCATTAAT
    GGCGATATCA AAGTCTTTCC AGATCCACCT CCTCCGGTAC GCCCTCCGCC TGTGGAAAAG
    GAGGAAAATG GCACCCTGAC CATGGAGCAG CTTGACAACC TCCGGGATCA GTTCTTAGAT
    ATAGCGCCCA AAGGCAGGGA TCCTCCAGAC AATGTGAGTG GATTCACCAA GAAGCAGAAA
    AGGCCTCGGA AGCTTGACCA TTACCTCCCA AAGAGGAACA ATCAATTTTT GAAAATTTGG
    CTTAAAAAAT ACCCTTGGCT TGTATACGAT GAGATACTAA ATCTTATGTT CTGCGGCCTG
    TGTCGTAAAC ATGGAGTGAC ATTAAAGGGA AATCAGGTCA GTTTTTTTTA TGGCACGGAT
    AACTTCAGGA CTGAATTTCT CAACGCGCAT CATCTGAGTG AGGCTCACGC CAAGGCGTCC
    CTCATGGAAG CCACGAGTGG TTCTCCAGGG AGCAGAGCTA CCACGGAGCT GATGGTGAGG
    ACCATGAGCA AGGTGACCCT TGGCAGGGTG GAGAACCTGT TCCGGTCCTG CCACGCCATC
    GCCAAGACCG GACGTCCCCT CAAAGACTTC CTCTGGATGT GTTCGCTGGA CGATATGAAG
    GGAGTTGATA TCGGCCCGGT GTTCAGAACC AGTAAATCGG CCAGAACTTT TACCTACTTT
    ATTGCTGAAG TGGAACGGAG AACTCTAAGA GAGAAGTTAG AAAAAAGTCA GTTCTTCTCC
    GTCCTCAGTG ATGGGATTGC AGACAGTTCA GCTGAGGAAG CTGAACTCGT ATATGTGCAG
    TTTGCCCGCG CAGGGAGAGT GCACTGCCAA ATCGTCGGGG TACAGACCGT GGAGAAGAAG
    GAGCCCTGGG CAATAAAACG TGCCATCGAG AAAACTCTGG AGAGAAATCT TCAACTTCGA
    CTGTCGAACC AAGACTGGGC AAAGAAACTG GTGGGTTTCG GAAGCGATGG AGCTCCAGGT
    TCAGAGGGAG AAAACGGCAG CACAGTGGCC CTGCTTTTAA GGGAAATCCA GCCAGGTGTG
    CTGCCTGTTT ACTGCCTTGC CCATCACCTC GAACTGTCTT ACCAGGCGGT GTTCCAGAGC
    GTTCCCCTCT ACAGCCGTGT GAAAGACCTT CTTCATAGCA TGCACCGCTT CTATCACGAT
    TCTCCTGCTC ACAGGAGTGC TCTGGTGACT GCTTTCAAGG GCCTCCACCT TCAACCTGCG
    ATGCCCTCTC AAGTCAGAGG CCGGAGGTGG CTTCAGGGAT TGCAGGCTGC CCTCCAGAAC
    TTTCTCAAGG GATATCCAGC AATTGTCCAG CAGCTGCAGT CAACAGAAGA AAGCAGAAGC
    AACAGCAGTC ATCAGAAAGC CGAAGAACTC CTGGAGTCTC TCCTCCAGGC AGACATTGTC
    AAATTTGTCC ACTTCTTGGT GGATGTCGTC AATGTCCTTA GCATACTGTC CCATGTCAAT
    CAGAACAGAA ATTCCTCAAT TGCTGATGTA TTTGCCACCT TAGAGTCAAC ACTGGAAATG
    CTCCAAATTT ATCAGACAAG ACCAGGGCCG AAAGAACGTG GGGTAGATTC GGTCACACAC
    TTTCATGGTA ACTGCCTTGG AGGAAAGGGA AACATTTCTG ACGTGAGAAA CATTGTTTTA
    ACACATCTTA TCAAGAGGCT GCGAGGCTGT TTCAGAGATG CCAACCAAGA TGTGGTGAAG
    GCAACCACGG TTGGAAGCTT TAAACTGTGG CCGGCAAAAA TAAATCAAGA ATTTGGAGAA
    AAAGAGGTGG CCATCCTGAT TGCACATTAT GAACCAGTAT TAGAGGCAGC CAAGGTGAAA
    ATTGACCAAG TAGACACAGA ATGGAGCATG TTGAAATTGG AGATCTACTC TAGATTTCAG
    AACATTTGCA AACTGACCTG GGATTTTGTG AATTCTGTTT ATTTACACAA GTATCCAAAC
    ATCCTGACAC TCATTGACCT TGTGCTGAGC CTGCCTGCAA GTTCAGCTGA GGCAGGACGT
    GGCTTTAGTC AGATGAAGCG CATCAGGTCC CAAATGCATG CCAAGGTCAA GGCTGACAAC
    ATGACGGATC TCCTGATAAT TCAGTTGAAT TCTCCAGACA TTAACAACTT TGACCCTAGG
    AAAGCCATTC ATTTATGGAA TACAAGAACA CCATCCTCAA CTGAGGACAC AAGACCTAAC
    TCAGACTGCT CATCAAACTC AGAAAGCCTT AGTGAAAGCG ATAAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OOv13488
    Clone ID Related Accession (Same CDS sequence) XM_012129857.2 , XM_012129859.2
    Accession Version XM_012129857.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5289bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-16
    Organism Ovis aries musimon(Ovis orientalis musimon)
    Product sperm flagellar protein 2 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942355.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012129857.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGTCGGAAA TCCTGTGCCA GTGGCTCAAC CAGGAATTGA AGGTGTCCCA GACCGTGAGT 
    CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAA TTGGAGAAGT TTTGCACAAG
    TTTGCACTTC AGGATGATTT TGCAGACTTT TCAGAAAGCA GGATTTCCAG TGCCAGACTT
    AACAATTTTT CTCGCTTGGA GCCCACACTT CACCTTTTGG GTGTGGAATT TGATCAGAAT
    GTGGCCCACA ATATCATCAC AGAGAAGCCT GGGGCTGCCA CGAAACTCTT ATATCAGTTG
    TACATTGCTC TCCAGAGGAA AAAGAAAGGT GTTCAGACTA GAGTGGAAAT GCCAACTGTA
    CAGCCCCTGA TGAGCACAAA AGTTCAAAAC ATGAAAAGCG AGGCTTTCCG AGATCAACGC
    TTGAGCAAAA GACGACAAAA TGAAATCATG GCCAAAATCC AAGCAGCTAT CATACAGATT
    CCTAAACCCA CATCAAATCG TACTCTGAAA ACATTTGACG CCCAAAAATT AATGAAAAAG
    AAAAAAGAGG CAGAGGATGT GGCCAATGAA ATTAAGAAGT TTGAAGCATT AATAAAAAAG
    GATCTCCAGG CAAAAGAAAG TGCATCCAAG ACTTCTCTAG ATACAGCAGG CCAGACAACC
    GCTGATTTGT TAAACACTTA TTCAGATGAT GACTACATTA AAAAAATCCA AAAACGCCTT
    GAAGAAGATG CTTTTGCACG AGAGCAAAGG GAGAAAAGAC GGCGGAGACT CTTAATGGAT
    CAGTTAATAG CTCATGAAGC CCAAGAGGAG GCTTATCGAG AGGAGCAGTT GATCAACCGG
    CTGATGCGAC AGTCCCAGCA GGAACGCAGG ATCGCTGTGC AGCTCATGCA CGTTAGGCAC
    GAAAAGGAAG TTTTATGGCA AAACAGAATT TTCAGAGAAA AACAATATGA GGAAAGACGG
    CTTAAAGACT TCCAGGATGC TCTTGATCGA GAAGCGGCTT TAGCCAGACA AGCCAAGATT
    GATTTTGAAG AACAAGCCCT CAGAGATAAG GAAATTCATG AGCAGATCGC CATAGAAAGA
    GCTCAAGCTC GTTACAAAAA GCATTATTCA ATATGTGCAG AAATTTTGGA TCAGATACTT
    GATCTGTCCA CTAAGGTGGC AGACTATCGA TTGTTGACAA ATAACCTGAT CCCATATAAG
    TTGATGCATG ATTGGAAGGA ACTGTTTTTC CATGGAAAAC CCATATATGA GCAAGCCTCT
    CTTAGACATT TACCAGCTAA GCTCTCTGCA GAACACCTTG TAGAAATGGA GAAAAGGAAC
    TTGCTAGATA ACAATGATTA TGAAGAATAT AAGAACATGG TTGGAGAATG GGCCTTACCA
    GAAGAAATGG TTGAAAACTT ACCACCCTCC AACAATTGCA TATTGGGCCA TGTGATTCAC
    AGACTAATTG AAAGTTCTCT TCCTCCTCAA GTTGAATCTA CAGTACCTGA GTTACCTTCA
    TTTGCTATAA AAGGATGCTT GTTGGGGAAA ACCTTTAGTG GAAAAACTAC TGCTCTAAAG
    GCTCTAGAGA AAAGCTTTCC TATTCAGGTA CTTTCTATTG ACACTCTTGT TCAAGAAGCT
    ATCCAAGCAT TTCATGACAA TGAAAGTATC AGCGGGGCCC ATGGACTTCA GAAAGCACCT
    GAAGAAAAGG TTTTACCAGT TAGGCGAAAG AGTGGTAAAG AAAACCAGGA CGTGCAAGAT
    GAAGCACTAC CAGAAAGAGA AGATAAAACC AGACTTGATA CCGATGCTAG AAAAGCAGCA
    AAAGCTGAAG AAGTCGTAGC GAGTGATAGT TTCTCTGAAC TCACTGTGCG TGCTCAGCTT
    GGTGCAAAAT CAGAAATGCT GCTGAAGAAA GGAAAGAGCA TATCTGATAT ATTGCTAGTC
    AGCATCATGG TAAATGCCAT TAATCAGATA CCTATGTATA AAGGCTGGAT CCTGGATGGC
    TTTCCAATGA CATTAAACCA AGCAAAACTT CTGGAGGAAG CACTCACAGG CTACAACAGA
    AACCTCATAG AACTGGAGGG AAAGAAATCA CAAACATCCA CATTAGCTGT TGACCCTACA
    GCTTCCAAAG AAGTGCCTCT TCCCCCTTCT GCATTTGATT TTGTCATGTT ATTAGACATT
    TCAGATAATT CCTCACTGAA TCGCATGAAT GACATCATGG CTAAAGCATT TTCAAGTGAA
    GCTCCTCGTG AAGATATCAA TCAGCGTGTA GCTGCTAAAA ACAAAGATAC AGATGAGGAC
    CAGAATTTAA GAGATCAAAT ACAGCACAGA ATTGTAGGCT TCCTGGACAA CTGGCCTTCG
    TTGGAGCAGT GGTTTACAGA GCCAGAAAAC ATTTTGATAA AAATCAATGC TGAAGTAGAT
    AAACAGTCAT TGTTCCAAAA AGTAAAGGAA ATGATTATGA CTGAAATAAT TAAAAAAGAA
    AATAAAGTGA AAAAGAAATT AGAAGAAAAG GAAGCTGAGA GAAAAGAAGA AGTTTCCCTG
    AGTGAGAAGA CATCCCCTCC TCCTCCTCCC CCTCCGGAAC CAGAGAAAGA GAAGGAAGTC
    CACCTTCCAC AGGAGCGGTC CAAAACTCCT GCTGCAAAAG GAAAGCAAGC ATCAGAACCC
    CTGCATGGTA ATCGAGAACC TCTTCAGGAA GGAAAAGGGA AGAAAAGTGA AACTTCACTC
    AAAAGAAAAG GTTCTCCTAG AGGAAAATCG CTAGGAGGGA AGATTCCACT CAAGAAATCA
    CCTGCCGAGT CTACAGATAT TTCACCGGTC CCGGTAGTGC CAGCCCCATC GAAGCCCGGA
    TCAGAAGAGT GGGTGTATGT AAATGAACCA ATTCCTGAGG AAATACCATC ATTTTTAGTA
    CCATACTGGA AATTGATAGA AAATTCCTAT ATAAATTCCA TCAAAACAGT ACTCAGGGAG
    TTGAGAGAAG GCCAGCATAG TGTACTTGCT TACTTATATG ATATTAGAAC AGGCTTCCAG
    CAGTTTCTGA AGCGTCCAGA CCACAAGCAA GACTTCGTGT CTCAGTGGCA GGCGGATTTC
    AACTCCCTTC CTGATGACCT GTGGGATGAT GAGGAAACAA AGGCTGAGCT GCACCAAAGA
    GTGAATGATT TACGGGACCG CCTGTGGGAC ATTTGCGATG CCCGTAAGGA AGAAGCAGAG
    CAGGAGCGTC TGGACATCAT TAACGAGAGC TGGTTGCAGG ACTCAATGGG AATTGCCATG
    AACCATTTCT TCTCACTGAT GCAGGCAGAA CTGAACCGTT TCCAAGATAC GAAGAGATTC
    CTGCAAGATT ATTACAGGGC CATGGAATGT AAAATTCCGA TGGATGACAC TAAGAAATTC
    ACTCGAGTCC CACTGGTCCG ACTGGATAGT AGAGATATTT TGGAAAGCCA GCTTAGAATT
    CCCCTGGTTC CTCGAAGATC CATTTCTCCA GAGTCAGCTT TATCCAAACC AAAAACAAAA
    AGCATCCTGA AGGGCAAGAT CGATTACACG CTGGAAAGTG TAGAGTTAAA CTTTGAGACT
    GATGAGAAGA TGGTGATGGA TACGTGGCAG CAGTCTTCTT TGGCAGTATC TCACATGGTG
    GCTGCTGAAA TTCATCAGAG GCTCATGGAA GAAGAAAAAG AAAACCAGCC AGCAGAAACA
    AAAGAAAAAC TTCCTCAGGC AGCTGCAAAT AAAAAAGTGA AAAAGGAGCC TCCGCCACCG
    CCACCACCAC CCAAGAAAAA GAAAGAGGAC AAAAAAGGAA AAGGTAAATC CCCACCTATG
    GCAGAAGCTA CTCCGGTAGT AGTGCCAGCA GAAGAGACTG AGGAAGTGGA ATTAAAAAAT
    GAACTGAAGC TCAGAATAAA GGAAGAGCAT CTTGCTGCCC TGCAATTTGA AGAGATAGCT
    ACACAGTTCC GACTTGAACT GATAAAGGTA AAAGCATTGG CTTTCCTGGA AGAGTTAGTA
    ATAAAGGCGG TTGATGTATA TAGACTCATG GACAAGTGGC TCGGTGAGAG GTACTTGAAT
    GAAATGGCCA GTGTGGAAAA ACTAACTGGG GTAGCTCGCT ATCACATTGA AACATCAACC
    AAAATTCAGA ATGAACTTTA TTTAGACCAA GAAGACTTCT TCATTAATGG CGATATCAAA
    GTCTTTCCAG ATCCACCTCC TCCGGTACGC CCTCCGCCTG TGGAAAAGGA GGAAAATGGC
    ACCCTGACCA TGGAGCAGCT TGACAACCTC CGGGATCAGT TCTTAGATAT AGCGCCCAAA
    GGTATAATAG GAAATAAAGC ATTTGCTGAC ATTCTGCTGG ATTTGGTAAC TCTGAACCTT
    GGAACAAACA ACTTTCCTAC TAGTTGGATG AACCTCACTC AACCGGAATT GCAGGAATTA
    GCATCTTTGT TGGTAACAAA CTCGGAGTCC GTGGACTGGC GGAAATTCTT GTTAGTCGTA
    GCGCTGCCTT GGCCCATCCC CCTGGAGGAG GAGCTTCTGG AGACCCTGCA GAGGTTCAAG
    GCCCTGGATG CAGAGCAGCT GGGCACCGTC ACTTATGAAC AGTATAAGCA GGCTGGCCTG
    TGGTTTACAG GAGATGAAGA TATCAAAATT CCAGAAAATC CTCTTGAACC CCTGCCATTC
    AATAGGCAGG AGCATCTTAT AGAGTTTTTC TTTAGGTTGT TTGCTGACTG CGAGAAGGTT
    CCACCCCAGC TTGACTACAC ACAGATGCTG CTTTATTTTG CTTGCCATCC AGACAGCACA
    GAAGGGGTCT ACAGGGCCCT CAGCGTAGCC ATTGGGACTC ACGTCTTCCA GCCAATTGAA
    ATGCCTCATC CTGTTGCAGC AAAGACCTCT TCCTTTACTG ATATGAGTCC CATTGAGGAA
    TATCCTGAAC TCGAGGACAA CTTTATGAGT GAGGAAAGGG AATTACAAGA GGACGGGGAG
    GAAAAGGAAG AAGAAATCCC TGAAAATGCA AATACGGAAA TGATTTCCAT GCAAACATTA
    CTCCAAGTGT TCCGAGGAGG AAATGAAGTA CTGGACGCTA ACAGATTTGC CAGCCACCAA
    AAGATAGAGA ACATTTATTT AGAGAACTTC ATAAAAGTGT TTCAGGACCT AGGTTCCAAG
    AACCTTGAGC CAATTGAAGT TGCTGTTCTT CTGAAGCATC CTTTTATTCA AGACCTGATT
    TCAAGTTATC CAGACTATAA GATCCCTGGT GCTTCTGCCG AGTCGGCTTC TTCAACCCTC
    CCCCGTCCTG GTCTTTCATC TCTCCTCCCT GTCATCACTT CACCCTGGAA TCTGACAAGA
    GGACAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011985247.1
    CDS493..5781
    Translation

    Target ORF information:

    RefSeq Version XM_012129857.2
    Organism Ovis aries musimon(Ovis orientalis musimon)
    Definition Ovis aries musimon sperm flagellar 2 (SPEF2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012129857.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGTCGGAAA TCCTGTGCCA GTGGCTCAAC CAGGAATTGA AGGTGTCCCA GACCGTGAGT 
    CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAA TTGGAGAAGT TTTGCACAAG
    TTTGCACTTC AGGATGATTT TGCAGACTTT TCAGAAAGCA GGATTTCCAG TGCCAGACTT
    AACAATTTTT CTCGCTTGGA GCCCACACTT CACCTTTTGG GTGTGGAATT TGATCAGAAT
    GTGGCCCACA ATATCATCAC AGAGAAGCCT GGGGCTGCCA CGAAACTCTT ATATCAGTTG
    TACATTGCTC TCCAGAGGAA AAAGAAAGGT GTTCAGACTA GAGTGGAAAT GCCAACTGTA
    CAGCCCCTGA TGAGCACAAA AGTTCAAAAC ATGAAAAGCG AGGCTTTCCG AGATCAACGC
    TTGAGCAAAA GACGACAAAA TGAAATCATG GCCAAAATCC AAGCAGCTAT CATACAGATT
    CCTAAACCCA CATCAAATCG TACTCTGAAA ACATTTGACG CCCAAAAATT AATGAAAAAG
    AAAAAAGAGG CAGAGGATGT GGCCAATGAA ATTAAGAAGT TTGAAGCATT AATAAAAAAG
    GATCTCCAGG CAAAAGAAAG TGCATCCAAG ACTTCTCTAG ATACAGCAGG CCAGACAACC
    GCTGATTTGT TAAACACTTA TTCAGATGAT GACTACATTA AAAAAATCCA AAAACGCCTT
    GAAGAAGATG CTTTTGCACG AGAGCAAAGG GAGAAAAGAC GGCGGAGACT CTTAATGGAT
    CAGTTAATAG CTCATGAAGC CCAAGAGGAG GCTTATCGAG AGGAGCAGTT GATCAACCGG
    CTGATGCGAC AGTCCCAGCA GGAACGCAGG ATCGCTGTGC AGCTCATGCA CGTTAGGCAC
    GAAAAGGAAG TTTTATGGCA AAACAGAATT TTCAGAGAAA AACAATATGA GGAAAGACGG
    CTTAAAGACT TCCAGGATGC TCTTGATCGA GAAGCGGCTT TAGCCAGACA AGCCAAGATT
    GATTTTGAAG AACAAGCCCT CAGAGATAAG GAAATTCATG AGCAGATCGC CATAGAAAGA
    GCTCAAGCTC GTTACAAAAA GCATTATTCA ATATGTGCAG AAATTTTGGA TCAGATACTT
    GATCTGTCCA CTAAGGTGGC AGACTATCGA TTGTTGACAA ATAACCTGAT CCCATATAAG
    TTGATGCATG ATTGGAAGGA ACTGTTTTTC CATGGAAAAC CCATATATGA GCAAGCCTCT
    CTTAGACATT TACCAGCTAA GCTCTCTGCA GAACACCTTG TAGAAATGGA GAAAAGGAAC
    TTGCTAGATA ACAATGATTA TGAAGAATAT AAGAACATGG TTGGAGAATG GGCCTTACCA
    GAAGAAATGG TTGAAAACTT ACCACCCTCC AACAATTGCA TATTGGGCCA TGTGATTCAC
    AGACTAATTG AAAGTTCTCT TCCTCCTCAA GTTGAATCTA CAGTACCTGA GTTACCTTCA
    TTTGCTATAA AAGGATGCTT GTTGGGGAAA ACCTTTAGTG GAAAAACTAC TGCTCTAAAG
    GCTCTAGAGA AAAGCTTTCC TATTCAGGTA CTTTCTATTG ACACTCTTGT TCAAGAAGCT
    ATCCAAGCAT TTCATGACAA TGAAAGTATC AGCGGGGCCC ATGGACTTCA GAAAGCACCT
    GAAGAAAAGG TTTTACCAGT TAGGCGAAAG AGTGGTAAAG AAAACCAGGA CGTGCAAGAT
    GAAGCACTAC CAGAAAGAGA AGATAAAACC AGACTTGATA CCGATGCTAG AAAAGCAGCA
    AAAGCTGAAG AAGTCGTAGC GAGTGATAGT TTCTCTGAAC TCACTGTGCG TGCTCAGCTT
    GGTGCAAAAT CAGAAATGCT GCTGAAGAAA GGAAAGAGCA TATCTGATAT ATTGCTAGTC
    AGCATCATGG TAAATGCCAT TAATCAGATA CCTATGTATA AAGGCTGGAT CCTGGATGGC
    TTTCCAATGA CATTAAACCA AGCAAAACTT CTGGAGGAAG CACTCACAGG CTACAACAGA
    AACCTCATAG AACTGGAGGG AAAGAAATCA CAAACATCCA CATTAGCTGT TGACCCTACA
    GCTTCCAAAG AAGTGCCTCT TCCCCCTTCT GCATTTGATT TTGTCATGTT ATTAGACATT
    TCAGATAATT CCTCACTGAA TCGCATGAAT GACATCATGG CTAAAGCATT TTCAAGTGAA
    GCTCCTCGTG AAGATATCAA TCAGCGTGTA GCTGCTAAAA ACAAAGATAC AGATGAGGAC
    CAGAATTTAA GAGATCAAAT ACAGCACAGA ATTGTAGGCT TCCTGGACAA CTGGCCTTCG
    TTGGAGCAGT GGTTTACAGA GCCAGAAAAC ATTTTGATAA AAATCAATGC TGAAGTAGAT
    AAACAGTCAT TGTTCCAAAA AGTAAAGGAA ATGATTATGA CTGAAATAAT TAAAAAAGAA
    AATAAAGTGA AAAAGAAATT AGAAGAAAAG GAAGCTGAGA GAAAAGAAGA AGTTTCCCTG
    AGTGAGAAGA CATCCCCTCC TCCTCCTCCC CCTCCGGAAC CAGAGAAAGA GAAGGAAGTC
    CACCTTCCAC AGGAGCGGTC CAAAACTCCT GCTGCAAAAG GAAAGCAAGC ATCAGAACCC
    CTGCATGGTA ATCGAGAACC TCTTCAGGAA GGAAAAGGGA AGAAAAGTGA AACTTCACTC
    AAAAGAAAAG GTTCTCCTAG AGGAAAATCG CTAGGAGGGA AGATTCCACT CAAGAAATCA
    CCTGCCGAGT CTACAGATAT TTCACCGGTC CCGGTAGTGC CAGCCCCATC GAAGCCCGGA
    TCAGAAGAGT GGGTGTATGT AAATGAACCA ATTCCTGAGG AAATACCATC ATTTTTAGTA
    CCATACTGGA AATTGATAGA AAATTCCTAT ATAAATTCCA TCAAAACAGT ACTCAGGGAG
    TTGAGAGAAG GCCAGCATAG TGTACTTGCT TACTTATATG ATATTAGAAC AGGCTTCCAG
    CAGTTTCTGA AGCGTCCAGA CCACAAGCAA GACTTCGTGT CTCAGTGGCA GGCGGATTTC
    AACTCCCTTC CTGATGACCT GTGGGATGAT GAGGAAACAA AGGCTGAGCT GCACCAAAGA
    GTGAATGATT TACGGGACCG CCTGTGGGAC ATTTGCGATG CCCGTAAGGA AGAAGCAGAG
    CAGGAGCGTC TGGACATCAT TAACGAGAGC TGGTTGCAGG ACTCAATGGG AATTGCCATG
    AACCATTTCT TCTCACTGAT GCAGGCAGAA CTGAACCGTT TCCAAGATAC GAAGAGATTC
    CTGCAAGATT ATTACAGGGC CATGGAATGT AAAATTCCGA TGGATGACAC TAAGAAATTC
    ACTCGAGTCC CACTGGTCCG ACTGGATAGT AGAGATATTT TGGAAAGCCA GCTTAGAATT
    CCCCTGGTTC CTCGAAGATC CATTTCTCCA GAGTCAGCTT TATCCAAACC AAAAACAAAA
    AGCATCCTGA AGGGCAAGAT CGATTACACG CTGGAAAGTG TAGAGTTAAA CTTTGAGACT
    GATGAGAAGA TGGTGATGGA TACGTGGCAG CAGTCTTCTT TGGCAGTATC TCACATGGTG
    GCTGCTGAAA TTCATCAGAG GCTCATGGAA GAAGAAAAAG AAAACCAGCC AGCAGAAACA
    AAAGAAAAAC TTCCTCAGGC AGCTGCAAAT AAAAAAGTGA AAAAGGAGCC TCCGCCACCG
    CCACCACCAC CCAAGAAAAA GAAAGAGGAC AAAAAAGGAA AAGGTAAATC CCCACCTATG
    GCAGAAGCTA CTCCGGTAGT AGTGCCAGCA GAAGAGACTG AGGAAGTGGA ATTAAAAAAT
    GAACTGAAGC TCAGAATAAA GGAAGAGCAT CTTGCTGCCC TGCAATTTGA AGAGATAGCT
    ACACAGTTCC GACTTGAACT GATAAAGGTA AAAGCATTGG CTTTCCTGGA AGAGTTAGTA
    ATAAAGGCGG TTGATGTATA TAGACTCATG GACAAGTGGC TCGGTGAGAG GTACTTGAAT
    GAAATGGCCA GTGTGGAAAA ACTAACTGGG GTAGCTCGCT ATCACATTGA AACATCAACC
    AAAATTCAGA ATGAACTTTA TTTAGACCAA GAAGACTTCT TCATTAATGG CGATATCAAA
    GTCTTTCCAG ATCCACCTCC TCCGGTACGC CCTCCGCCTG TGGAAAAGGA GGAAAATGGC
    ACCCTGACCA TGGAGCAGCT TGACAACCTC CGGGATCAGT TCTTAGATAT AGCGCCCAAA
    GGTATAATAG GAAATAAAGC ATTTGCTGAC ATTCTGCTGG ATTTGGTAAC TCTGAACCTT
    GGAACAAACA ACTTTCCTAC TAGTTGGATG AACCTCACTC AACCGGAATT GCAGGAATTA
    GCATCTTTGT TGGTAACAAA CTCGGAGTCC GTGGACTGGC GGAAATTCTT GTTAGTCGTA
    GCGCTGCCTT GGCCCATCCC CCTGGAGGAG GAGCTTCTGG AGACCCTGCA GAGGTTCAAG
    GCCCTGGATG CAGAGCAGCT GGGCACCGTC ACTTATGAAC AGTATAAGCA GGCTGGCCTG
    TGGTTTACAG GAGATGAAGA TATCAAAATT CCAGAAAATC CTCTTGAACC CCTGCCATTC
    AATAGGCAGG AGCATCTTAT AGAGTTTTTC TTTAGGTTGT TTGCTGACTG CGAGAAGGTT
    CCACCCCAGC TTGACTACAC ACAGATGCTG CTTTATTTTG CTTGCCATCC AGACAGCACA
    GAAGGGGTCT ACAGGGCCCT CAGCGTAGCC ATTGGGACTC ACGTCTTCCA GCCAATTGAA
    ATGCCTCATC CTGTTGCAGC AAAGACCTCT TCCTTTACTG ATATGAGTCC CATTGAGGAA
    TATCCTGAAC TCGAGGACAA CTTTATGAGT GAGGAAAGGG AATTACAAGA GGACGGGGAG
    GAAAAGGAAG AAGAAATCCC TGAAAATGCA AATACGGAAA TGATTTCCAT GCAAACATTA
    CTCCAAGTGT TCCGAGGAGG AAATGAAGTA CTGGACGCTA ACAGATTTGC CAGCCACCAA
    AAGATAGAGA ACATTTATTT AGAGAACTTC ATAAAAGTGT TTCAGGACCT AGGTTCCAAG
    AACCTTGAGC CAATTGAAGT TGCTGTTCTT CTGAAGCATC CTTTTATTCA AGACCTGATT
    TCAAGTTATC CAGACTATAA GATCCCTGGT GCTTCTGCCG AGTCGGCTTC TTCAACCCTC
    CCCCGTCCTG GTCTTTCATC TCTCCTCCCT GTCATCACTT CACCCTGGAA TCTGACAAGA
    GGACAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OOv13488
    Clone ID Related Accession (Same CDS sequence) XM_012129857.2 , XM_012129859.2
    Accession Version XM_012129859.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5289bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-16
    Organism Ovis aries musimon(Ovis orientalis musimon)
    Product sperm flagellar protein 2 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942355.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012129859.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGTCGGAAA TCCTGTGCCA GTGGCTCAAC CAGGAATTGA AGGTGTCCCA GACCGTGAGT 
    CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAA TTGGAGAAGT TTTGCACAAG
    TTTGCACTTC AGGATGATTT TGCAGACTTT TCAGAAAGCA GGATTTCCAG TGCCAGACTT
    AACAATTTTT CTCGCTTGGA GCCCACACTT CACCTTTTGG GTGTGGAATT TGATCAGAAT
    GTGGCCCACA ATATCATCAC AGAGAAGCCT GGGGCTGCCA CGAAACTCTT ATATCAGTTG
    TACATTGCTC TCCAGAGGAA AAAGAAAGGT GTTCAGACTA GAGTGGAAAT GCCAACTGTA
    CAGCCCCTGA TGAGCACAAA AGTTCAAAAC ATGAAAAGCG AGGCTTTCCG AGATCAACGC
    TTGAGCAAAA GACGACAAAA TGAAATCATG GCCAAAATCC AAGCAGCTAT CATACAGATT
    CCTAAACCCA CATCAAATCG TACTCTGAAA ACATTTGACG CCCAAAAATT AATGAAAAAG
    AAAAAAGAGG CAGAGGATGT GGCCAATGAA ATTAAGAAGT TTGAAGCATT AATAAAAAAG
    GATCTCCAGG CAAAAGAAAG TGCATCCAAG ACTTCTCTAG ATACAGCAGG CCAGACAACC
    GCTGATTTGT TAAACACTTA TTCAGATGAT GACTACATTA AAAAAATCCA AAAACGCCTT
    GAAGAAGATG CTTTTGCACG AGAGCAAAGG GAGAAAAGAC GGCGGAGACT CTTAATGGAT
    CAGTTAATAG CTCATGAAGC CCAAGAGGAG GCTTATCGAG AGGAGCAGTT GATCAACCGG
    CTGATGCGAC AGTCCCAGCA GGAACGCAGG ATCGCTGTGC AGCTCATGCA CGTTAGGCAC
    GAAAAGGAAG TTTTATGGCA AAACAGAATT TTCAGAGAAA AACAATATGA GGAAAGACGG
    CTTAAAGACT TCCAGGATGC TCTTGATCGA GAAGCGGCTT TAGCCAGACA AGCCAAGATT
    GATTTTGAAG AACAAGCCCT CAGAGATAAG GAAATTCATG AGCAGATCGC CATAGAAAGA
    GCTCAAGCTC GTTACAAAAA GCATTATTCA ATATGTGCAG AAATTTTGGA TCAGATACTT
    GATCTGTCCA CTAAGGTGGC AGACTATCGA TTGTTGACAA ATAACCTGAT CCCATATAAG
    TTGATGCATG ATTGGAAGGA ACTGTTTTTC CATGGAAAAC CCATATATGA GCAAGCCTCT
    CTTAGACATT TACCAGCTAA GCTCTCTGCA GAACACCTTG TAGAAATGGA GAAAAGGAAC
    TTGCTAGATA ACAATGATTA TGAAGAATAT AAGAACATGG TTGGAGAATG GGCCTTACCA
    GAAGAAATGG TTGAAAACTT ACCACCCTCC AACAATTGCA TATTGGGCCA TGTGATTCAC
    AGACTAATTG AAAGTTCTCT TCCTCCTCAA GTTGAATCTA CAGTACCTGA GTTACCTTCA
    TTTGCTATAA AAGGATGCTT GTTGGGGAAA ACCTTTAGTG GAAAAACTAC TGCTCTAAAG
    GCTCTAGAGA AAAGCTTTCC TATTCAGGTA CTTTCTATTG ACACTCTTGT TCAAGAAGCT
    ATCCAAGCAT TTCATGACAA TGAAAGTATC AGCGGGGCCC ATGGACTTCA GAAAGCACCT
    GAAGAAAAGG TTTTACCAGT TAGGCGAAAG AGTGGTAAAG AAAACCAGGA CGTGCAAGAT
    GAAGCACTAC CAGAAAGAGA AGATAAAACC AGACTTGATA CCGATGCTAG AAAAGCAGCA
    AAAGCTGAAG AAGTCGTAGC GAGTGATAGT TTCTCTGAAC TCACTGTGCG TGCTCAGCTT
    GGTGCAAAAT CAGAAATGCT GCTGAAGAAA GGAAAGAGCA TATCTGATAT ATTGCTAGTC
    AGCATCATGG TAAATGCCAT TAATCAGATA CCTATGTATA AAGGCTGGAT CCTGGATGGC
    TTTCCAATGA CATTAAACCA AGCAAAACTT CTGGAGGAAG CACTCACAGG CTACAACAGA
    AACCTCATAG AACTGGAGGG AAAGAAATCA CAAACATCCA CATTAGCTGT TGACCCTACA
    GCTTCCAAAG AAGTGCCTCT TCCCCCTTCT GCATTTGATT TTGTCATGTT ATTAGACATT
    TCAGATAATT CCTCACTGAA TCGCATGAAT GACATCATGG CTAAAGCATT TTCAAGTGAA
    GCTCCTCGTG AAGATATCAA TCAGCGTGTA GCTGCTAAAA ACAAAGATAC AGATGAGGAC
    CAGAATTTAA GAGATCAAAT ACAGCACAGA ATTGTAGGCT TCCTGGACAA CTGGCCTTCG
    TTGGAGCAGT GGTTTACAGA GCCAGAAAAC ATTTTGATAA AAATCAATGC TGAAGTAGAT
    AAACAGTCAT TGTTCCAAAA AGTAAAGGAA ATGATTATGA CTGAAATAAT TAAAAAAGAA
    AATAAAGTGA AAAAGAAATT AGAAGAAAAG GAAGCTGAGA GAAAAGAAGA AGTTTCCCTG
    AGTGAGAAGA CATCCCCTCC TCCTCCTCCC CCTCCGGAAC CAGAGAAAGA GAAGGAAGTC
    CACCTTCCAC AGGAGCGGTC CAAAACTCCT GCTGCAAAAG GAAAGCAAGC ATCAGAACCC
    CTGCATGGTA ATCGAGAACC TCTTCAGGAA GGAAAAGGGA AGAAAAGTGA AACTTCACTC
    AAAAGAAAAG GTTCTCCTAG AGGAAAATCG CTAGGAGGGA AGATTCCACT CAAGAAATCA
    CCTGCCGAGT CTACAGATAT TTCACCGGTC CCGGTAGTGC CAGCCCCATC GAAGCCCGGA
    TCAGAAGAGT GGGTGTATGT AAATGAACCA ATTCCTGAGG AAATACCATC ATTTTTAGTA
    CCATACTGGA AATTGATAGA AAATTCCTAT ATAAATTCCA TCAAAACAGT ACTCAGGGAG
    TTGAGAGAAG GCCAGCATAG TGTACTTGCT TACTTATATG ATATTAGAAC AGGCTTCCAG
    CAGTTTCTGA AGCGTCCAGA CCACAAGCAA GACTTCGTGT CTCAGTGGCA GGCGGATTTC
    AACTCCCTTC CTGATGACCT GTGGGATGAT GAGGAAACAA AGGCTGAGCT GCACCAAAGA
    GTGAATGATT TACGGGACCG CCTGTGGGAC ATTTGCGATG CCCGTAAGGA AGAAGCAGAG
    CAGGAGCGTC TGGACATCAT TAACGAGAGC TGGTTGCAGG ACTCAATGGG AATTGCCATG
    AACCATTTCT TCTCACTGAT GCAGGCAGAA CTGAACCGTT TCCAAGATAC GAAGAGATTC
    CTGCAAGATT ATTACAGGGC CATGGAATGT AAAATTCCGA TGGATGACAC TAAGAAATTC
    ACTCGAGTCC CACTGGTCCG ACTGGATAGT AGAGATATTT TGGAAAGCCA GCTTAGAATT
    CCCCTGGTTC CTCGAAGATC CATTTCTCCA GAGTCAGCTT TATCCAAACC AAAAACAAAA
    AGCATCCTGA AGGGCAAGAT CGATTACACG CTGGAAAGTG TAGAGTTAAA CTTTGAGACT
    GATGAGAAGA TGGTGATGGA TACGTGGCAG CAGTCTTCTT TGGCAGTATC TCACATGGTG
    GCTGCTGAAA TTCATCAGAG GCTCATGGAA GAAGAAAAAG AAAACCAGCC AGCAGAAACA
    AAAGAAAAAC TTCCTCAGGC AGCTGCAAAT AAAAAAGTGA AAAAGGAGCC TCCGCCACCG
    CCACCACCAC CCAAGAAAAA GAAAGAGGAC AAAAAAGGAA AAGGTAAATC CCCACCTATG
    GCAGAAGCTA CTCCGGTAGT AGTGCCAGCA GAAGAGACTG AGGAAGTGGA ATTAAAAAAT
    GAACTGAAGC TCAGAATAAA GGAAGAGCAT CTTGCTGCCC TGCAATTTGA AGAGATAGCT
    ACACAGTTCC GACTTGAACT GATAAAGGTA AAAGCATTGG CTTTCCTGGA AGAGTTAGTA
    ATAAAGGCGG TTGATGTATA TAGACTCATG GACAAGTGGC TCGGTGAGAG GTACTTGAAT
    GAAATGGCCA GTGTGGAAAA ACTAACTGGG GTAGCTCGCT ATCACATTGA AACATCAACC
    AAAATTCAGA ATGAACTTTA TTTAGACCAA GAAGACTTCT TCATTAATGG CGATATCAAA
    GTCTTTCCAG ATCCACCTCC TCCGGTACGC CCTCCGCCTG TGGAAAAGGA GGAAAATGGC
    ACCCTGACCA TGGAGCAGCT TGACAACCTC CGGGATCAGT TCTTAGATAT AGCGCCCAAA
    GGTATAATAG GAAATAAAGC ATTTGCTGAC ATTCTGCTGG ATTTGGTAAC TCTGAACCTT
    GGAACAAACA ACTTTCCTAC TAGTTGGATG AACCTCACTC AACCGGAATT GCAGGAATTA
    GCATCTTTGT TGGTAACAAA CTCGGAGTCC GTGGACTGGC GGAAATTCTT GTTAGTCGTA
    GCGCTGCCTT GGCCCATCCC CCTGGAGGAG GAGCTTCTGG AGACCCTGCA GAGGTTCAAG
    GCCCTGGATG CAGAGCAGCT GGGCACCGTC ACTTATGAAC AGTATAAGCA GGCTGGCCTG
    TGGTTTACAG GAGATGAAGA TATCAAAATT CCAGAAAATC CTCTTGAACC CCTGCCATTC
    AATAGGCAGG AGCATCTTAT AGAGTTTTTC TTTAGGTTGT TTGCTGACTG CGAGAAGGTT
    CCACCCCAGC TTGACTACAC ACAGATGCTG CTTTATTTTG CTTGCCATCC AGACAGCACA
    GAAGGGGTCT ACAGGGCCCT CAGCGTAGCC ATTGGGACTC ACGTCTTCCA GCCAATTGAA
    ATGCCTCATC CTGTTGCAGC AAAGACCTCT TCCTTTACTG ATATGAGTCC CATTGAGGAA
    TATCCTGAAC TCGAGGACAA CTTTATGAGT GAGGAAAGGG AATTACAAGA GGACGGGGAG
    GAAAAGGAAG AAGAAATCCC TGAAAATGCA AATACGGAAA TGATTTCCAT GCAAACATTA
    CTCCAAGTGT TCCGAGGAGG AAATGAAGTA CTGGACGCTA ACAGATTTGC CAGCCACCAA
    AAGATAGAGA ACATTTATTT AGAGAACTTC ATAAAAGTGT TTCAGGACCT AGGTTCCAAG
    AACCTTGAGC CAATTGAAGT TGCTGTTCTT CTGAAGCATC CTTTTATTCA AGACCTGATT
    TCAAGTTATC CAGACTATAA GATCCCTGGT GCTTCTGCCG AGTCGGCTTC TTCAACCCTC
    CCCCGTCCTG GTCTTTCATC TCTCCTCCCT GTCATCACTT CACCCTGGAA TCTGACAAGA
    GGACAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011985249.1
    CDS493..5781
    Translation

    Target ORF information:

    RefSeq Version XM_012129859.2
    Organism Ovis aries musimon(Ovis orientalis musimon)
    Definition Ovis aries musimon sperm flagellar 2 (SPEF2), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012129859.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGTCGGAAA TCCTGTGCCA GTGGCTCAAC CAGGAATTGA AGGTGTCCCA GACCGTGAGT 
    CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAA TTGGAGAAGT TTTGCACAAG
    TTTGCACTTC AGGATGATTT TGCAGACTTT TCAGAAAGCA GGATTTCCAG TGCCAGACTT
    AACAATTTTT CTCGCTTGGA GCCCACACTT CACCTTTTGG GTGTGGAATT TGATCAGAAT
    GTGGCCCACA ATATCATCAC AGAGAAGCCT GGGGCTGCCA CGAAACTCTT ATATCAGTTG
    TACATTGCTC TCCAGAGGAA AAAGAAAGGT GTTCAGACTA GAGTGGAAAT GCCAACTGTA
    CAGCCCCTGA TGAGCACAAA AGTTCAAAAC ATGAAAAGCG AGGCTTTCCG AGATCAACGC
    TTGAGCAAAA GACGACAAAA TGAAATCATG GCCAAAATCC AAGCAGCTAT CATACAGATT
    CCTAAACCCA CATCAAATCG TACTCTGAAA ACATTTGACG CCCAAAAATT AATGAAAAAG
    AAAAAAGAGG CAGAGGATGT GGCCAATGAA ATTAAGAAGT TTGAAGCATT AATAAAAAAG
    GATCTCCAGG CAAAAGAAAG TGCATCCAAG ACTTCTCTAG ATACAGCAGG CCAGACAACC
    GCTGATTTGT TAAACACTTA TTCAGATGAT GACTACATTA AAAAAATCCA AAAACGCCTT
    GAAGAAGATG CTTTTGCACG AGAGCAAAGG GAGAAAAGAC GGCGGAGACT CTTAATGGAT
    CAGTTAATAG CTCATGAAGC CCAAGAGGAG GCTTATCGAG AGGAGCAGTT GATCAACCGG
    CTGATGCGAC AGTCCCAGCA GGAACGCAGG ATCGCTGTGC AGCTCATGCA CGTTAGGCAC
    GAAAAGGAAG TTTTATGGCA AAACAGAATT TTCAGAGAAA AACAATATGA GGAAAGACGG
    CTTAAAGACT TCCAGGATGC TCTTGATCGA GAAGCGGCTT TAGCCAGACA AGCCAAGATT
    GATTTTGAAG AACAAGCCCT CAGAGATAAG GAAATTCATG AGCAGATCGC CATAGAAAGA
    GCTCAAGCTC GTTACAAAAA GCATTATTCA ATATGTGCAG AAATTTTGGA TCAGATACTT
    GATCTGTCCA CTAAGGTGGC AGACTATCGA TTGTTGACAA ATAACCTGAT CCCATATAAG
    TTGATGCATG ATTGGAAGGA ACTGTTTTTC CATGGAAAAC CCATATATGA GCAAGCCTCT
    CTTAGACATT TACCAGCTAA GCTCTCTGCA GAACACCTTG TAGAAATGGA GAAAAGGAAC
    TTGCTAGATA ACAATGATTA TGAAGAATAT AAGAACATGG TTGGAGAATG GGCCTTACCA
    GAAGAAATGG TTGAAAACTT ACCACCCTCC AACAATTGCA TATTGGGCCA TGTGATTCAC
    AGACTAATTG AAAGTTCTCT TCCTCCTCAA GTTGAATCTA CAGTACCTGA GTTACCTTCA
    TTTGCTATAA AAGGATGCTT GTTGGGGAAA ACCTTTAGTG GAAAAACTAC TGCTCTAAAG
    GCTCTAGAGA AAAGCTTTCC TATTCAGGTA CTTTCTATTG ACACTCTTGT TCAAGAAGCT
    ATCCAAGCAT TTCATGACAA TGAAAGTATC AGCGGGGCCC ATGGACTTCA GAAAGCACCT
    GAAGAAAAGG TTTTACCAGT TAGGCGAAAG AGTGGTAAAG AAAACCAGGA CGTGCAAGAT
    GAAGCACTAC CAGAAAGAGA AGATAAAACC AGACTTGATA CCGATGCTAG AAAAGCAGCA
    AAAGCTGAAG AAGTCGTAGC GAGTGATAGT TTCTCTGAAC TCACTGTGCG TGCTCAGCTT
    GGTGCAAAAT CAGAAATGCT GCTGAAGAAA GGAAAGAGCA TATCTGATAT ATTGCTAGTC
    AGCATCATGG TAAATGCCAT TAATCAGATA CCTATGTATA AAGGCTGGAT CCTGGATGGC
    TTTCCAATGA CATTAAACCA AGCAAAACTT CTGGAGGAAG CACTCACAGG CTACAACAGA
    AACCTCATAG AACTGGAGGG AAAGAAATCA CAAACATCCA CATTAGCTGT TGACCCTACA
    GCTTCCAAAG AAGTGCCTCT TCCCCCTTCT GCATTTGATT TTGTCATGTT ATTAGACATT
    TCAGATAATT CCTCACTGAA TCGCATGAAT GACATCATGG CTAAAGCATT TTCAAGTGAA
    GCTCCTCGTG AAGATATCAA TCAGCGTGTA GCTGCTAAAA ACAAAGATAC AGATGAGGAC
    CAGAATTTAA GAGATCAAAT ACAGCACAGA ATTGTAGGCT TCCTGGACAA CTGGCCTTCG
    TTGGAGCAGT GGTTTACAGA GCCAGAAAAC ATTTTGATAA AAATCAATGC TGAAGTAGAT
    AAACAGTCAT TGTTCCAAAA AGTAAAGGAA ATGATTATGA CTGAAATAAT TAAAAAAGAA
    AATAAAGTGA AAAAGAAATT AGAAGAAAAG GAAGCTGAGA GAAAAGAAGA AGTTTCCCTG
    AGTGAGAAGA CATCCCCTCC TCCTCCTCCC CCTCCGGAAC CAGAGAAAGA GAAGGAAGTC
    CACCTTCCAC AGGAGCGGTC CAAAACTCCT GCTGCAAAAG GAAAGCAAGC ATCAGAACCC
    CTGCATGGTA ATCGAGAACC TCTTCAGGAA GGAAAAGGGA AGAAAAGTGA AACTTCACTC
    AAAAGAAAAG GTTCTCCTAG AGGAAAATCG CTAGGAGGGA AGATTCCACT CAAGAAATCA
    CCTGCCGAGT CTACAGATAT TTCACCGGTC CCGGTAGTGC CAGCCCCATC GAAGCCCGGA
    TCAGAAGAGT GGGTGTATGT AAATGAACCA ATTCCTGAGG AAATACCATC ATTTTTAGTA
    CCATACTGGA AATTGATAGA AAATTCCTAT ATAAATTCCA TCAAAACAGT ACTCAGGGAG
    TTGAGAGAAG GCCAGCATAG TGTACTTGCT TACTTATATG ATATTAGAAC AGGCTTCCAG
    CAGTTTCTGA AGCGTCCAGA CCACAAGCAA GACTTCGTGT CTCAGTGGCA GGCGGATTTC
    AACTCCCTTC CTGATGACCT GTGGGATGAT GAGGAAACAA AGGCTGAGCT GCACCAAAGA
    GTGAATGATT TACGGGACCG CCTGTGGGAC ATTTGCGATG CCCGTAAGGA AGAAGCAGAG
    CAGGAGCGTC TGGACATCAT TAACGAGAGC TGGTTGCAGG ACTCAATGGG AATTGCCATG
    AACCATTTCT TCTCACTGAT GCAGGCAGAA CTGAACCGTT TCCAAGATAC GAAGAGATTC
    CTGCAAGATT ATTACAGGGC CATGGAATGT AAAATTCCGA TGGATGACAC TAAGAAATTC
    ACTCGAGTCC CACTGGTCCG ACTGGATAGT AGAGATATTT TGGAAAGCCA GCTTAGAATT
    CCCCTGGTTC CTCGAAGATC CATTTCTCCA GAGTCAGCTT TATCCAAACC AAAAACAAAA
    AGCATCCTGA AGGGCAAGAT CGATTACACG CTGGAAAGTG TAGAGTTAAA CTTTGAGACT
    GATGAGAAGA TGGTGATGGA TACGTGGCAG CAGTCTTCTT TGGCAGTATC TCACATGGTG
    GCTGCTGAAA TTCATCAGAG GCTCATGGAA GAAGAAAAAG AAAACCAGCC AGCAGAAACA
    AAAGAAAAAC TTCCTCAGGC AGCTGCAAAT AAAAAAGTGA AAAAGGAGCC TCCGCCACCG
    CCACCACCAC CCAAGAAAAA GAAAGAGGAC AAAAAAGGAA AAGGTAAATC CCCACCTATG
    GCAGAAGCTA CTCCGGTAGT AGTGCCAGCA GAAGAGACTG AGGAAGTGGA ATTAAAAAAT
    GAACTGAAGC TCAGAATAAA GGAAGAGCAT CTTGCTGCCC TGCAATTTGA AGAGATAGCT
    ACACAGTTCC GACTTGAACT GATAAAGGTA AAAGCATTGG CTTTCCTGGA AGAGTTAGTA
    ATAAAGGCGG TTGATGTATA TAGACTCATG GACAAGTGGC TCGGTGAGAG GTACTTGAAT
    GAAATGGCCA GTGTGGAAAA ACTAACTGGG GTAGCTCGCT ATCACATTGA AACATCAACC
    AAAATTCAGA ATGAACTTTA TTTAGACCAA GAAGACTTCT TCATTAATGG CGATATCAAA
    GTCTTTCCAG ATCCACCTCC TCCGGTACGC CCTCCGCCTG TGGAAAAGGA GGAAAATGGC
    ACCCTGACCA TGGAGCAGCT TGACAACCTC CGGGATCAGT TCTTAGATAT AGCGCCCAAA
    GGTATAATAG GAAATAAAGC ATTTGCTGAC ATTCTGCTGG ATTTGGTAAC TCTGAACCTT
    GGAACAAACA ACTTTCCTAC TAGTTGGATG AACCTCACTC AACCGGAATT GCAGGAATTA
    GCATCTTTGT TGGTAACAAA CTCGGAGTCC GTGGACTGGC GGAAATTCTT GTTAGTCGTA
    GCGCTGCCTT GGCCCATCCC CCTGGAGGAG GAGCTTCTGG AGACCCTGCA GAGGTTCAAG
    GCCCTGGATG CAGAGCAGCT GGGCACCGTC ACTTATGAAC AGTATAAGCA GGCTGGCCTG
    TGGTTTACAG GAGATGAAGA TATCAAAATT CCAGAAAATC CTCTTGAACC CCTGCCATTC
    AATAGGCAGG AGCATCTTAT AGAGTTTTTC TTTAGGTTGT TTGCTGACTG CGAGAAGGTT
    CCACCCCAGC TTGACTACAC ACAGATGCTG CTTTATTTTG CTTGCCATCC AGACAGCACA
    GAAGGGGTCT ACAGGGCCCT CAGCGTAGCC ATTGGGACTC ACGTCTTCCA GCCAATTGAA
    ATGCCTCATC CTGTTGCAGC AAAGACCTCT TCCTTTACTG ATATGAGTCC CATTGAGGAA
    TATCCTGAAC TCGAGGACAA CTTTATGAGT GAGGAAAGGG AATTACAAGA GGACGGGGAG
    GAAAAGGAAG AAGAAATCCC TGAAAATGCA AATACGGAAA TGATTTCCAT GCAAACATTA
    CTCCAAGTGT TCCGAGGAGG AAATGAAGTA CTGGACGCTA ACAGATTTGC CAGCCACCAA
    AAGATAGAGA ACATTTATTT AGAGAACTTC ATAAAAGTGT TTCAGGACCT AGGTTCCAAG
    AACCTTGAGC CAATTGAAGT TGCTGTTCTT CTGAAGCATC CTTTTATTCA AGACCTGATT
    TCAAGTTATC CAGACTATAA GATCCCTGGT GCTTCTGCCG AGTCGGCTTC TTCAACCCTC
    CCCCGTCCTG GTCTTTCATC TCTCCTCCCT GTCATCACTT CACCCTGGAA TCTGACAAGA
    GGACAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OOv13489
    Clone ID Related Accession (Same CDS sequence) XM_012129860.2
    Accession Version XM_012129860.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5277bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-16
    Organism Ovis aries musimon(Ovis orientalis musimon)
    Product sperm flagellar protein 2 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942355.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012129860.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGTCGGAAA TCCTGTGCCA GTGGCTCAAC CAGGAATTGA AGGTGTCCCA GACCGTGAGT 
    CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAA TTGGAGAAGT TTTGCACAAG
    TTTGCACTTC AGGATGATTT TGCAGACTTT TCAGAAAGCA GGATTTCCAG TGCCAGACTT
    AACAATTTTT CTCGCTTGGA GCCCACACTT CACCTTTTGG GTGTGGAATT TGATCAGAAT
    GTGGCCCACA ATATCATCAC AGAGAAGCCT GGGGCTGCCA CGAAACTCTT ATATCAGTTG
    TACATTGCTC TCCAGAGGAA AAAGAAAGGT GTTCAGACTA GAGTGGAAAT GCCAACTGTA
    CAGCCCCTGA TGAGCACAAA AGTTCAAAAC ATGAAAAGCG AGGCTTTCCG AGATCAACGC
    TTGAGCAAAA GACGACAAAA TGAAATCATG GCCAAAATCC AAGCAGCTAT CATACAGATT
    CCTAAACCCA CATCAAATCG TACTCTGAAA ACATTTGACG CCCAAAAATT AATGAAAAAG
    AAAAAAGAGG CAGAGGATGT GGCCAATGAA ATTAAGAAGT TTGAAGCATT AATAAAAAAG
    GATCTCCAGG CAAAAGAAAG TGCATCCAAG ACTTCTCTAG ATACAGCAGG CCAGACAACC
    GCTGATTTGT TAAACACTTA TTCAGATGAT GACTACATTA AAAAAATCCA AAAACGCCTT
    GAAGAAGATG CTTTTGCACG AGAGCAAAGG GAGAAAAGAC GGCGGAGACT CTTAATGGAT
    CAGTTAATAG CTCATGAAGC CCAAGAGGAG GCTTATCGAG AGGAGCAGTT GATCAACCGG
    CTGATGCGAC AGTCCCAGCA GGAACGCAGG ATCGCTGTGC AGCTCATGCA CGTTAGGCAC
    GAAAAGGAAG TTTTATGGCA AAACAGAATT TTCAGAGAAA AACAATATGA GGAAAGACGG
    CTTAAAGACT TCCAGGATGC TCTTGATCGA GAAGCGGCTT TAGCCAGACA AGCCAAGATT
    GATTTTGAAG AACAAGCCCT CAGAGATAAG GAAATTCATG AGCAGATCGC CATAGAAAGA
    GCTCAAGCTC GTTACAAAAA GCATTATTCA ATATGTGCAG AAATTTTGGA TCAGATACTT
    GATCTGTCCA CTAAGGTGGC AGACTATCGA TTGTTGACAA ATAACCTGAT CCCATATAAG
    TTGATGCATG ATTGGAAGGA ACTGTTTTTC CATGGAAAAC CCATATATGA GCAAGCCTCT
    CTTAGACATT TACCAGCTAA GCTCTCTGCA GAACACCTTG TAGAAATGGA GAAAAGGAAC
    TTGCTAGATA ACAATGATTA TGAAGAATAT AAGAACATGG TTGGAGAATG GGCCTTACCA
    GAAGAAATGG TTGAAAACTT ACCACCCTCC AACAATTGCA TATTGGGCCA TGTGATTCAC
    AGACTAATTG AAAGTTCTCT TCCTCCTCAA GTTGAATCTA CAGTACCTGA GTTACCTTCA
    TTTGCTATAA AAGGATGCTT GTTGGGGAAA ACCTTTAGTG GAAAAACTAC TGCTCTAAAG
    GCTCTAGAGA AAAGCTTTCC TATTCAGGTA CTTTCTATTG ACACTCTTGT TCAAGAAGCT
    ATCCAAGCAT TTCATGACAA TGAAAGTATC AGCGGGGCCC ATGGACTTCA GAAAGCACCT
    GAAGAAAAGG TTTTACCAGT TAGGCGAAAG AGTGGTAAAG AAAACCAGGA CGTGCAAGAT
    GAAGCACTAC CAGAAAGAGA AGATAAAACC AGACTTGATA CCGATGCTAG AAAAGCAGCA
    AAAGCTGAAG AAGTCGTAGC GAGTGATAGT TTCTCTGAAC TCACTGTGCG TGCTCAGCTT
    GGTGCAAAAT CAGAAATGCT GCTGAAGAAA GGAAAGAGCA TATCTGATAT ATTGCTAGTC
    AGCATCATGG TAAATGCCAT TAATCAGATA CCTATGTATA AAGGCTGGAT CCTGGATGGC
    TTTCCAATGA CATTAAACCA AGCAAAACTT CTGGAGGAAG CACTCACAGG CTACAACAGA
    AACCTCATAG AACTGGAGGG AAAGAAATCA CAAACATCCA CATTAGCTGT TGACCCTACA
    GCTTCCAAAG AAGTGCCTCT TCCCCCTTCT GCATTTGATT TTGTCATGTT ATTAGACATT
    TCAGATAATT CCTCACTGAA TCGCATGAAT GACATCATGG CTAAAGCATT TTCAAGTGAA
    GCTCCTCGTG AAGATATCAA TCAGCGTGTA GCTGCTAAAA ACAAAGATAC AGATGAGGAC
    CAGAATTTAA GAGATCAAAT ACAGCACAGA ATTGTAGGCT TCCTGGACAA CTGGCCTTCG
    TTGGAGCAGT GGTTTACAGA GCCAGAAAAC ATTTTGATAA AAATCAATGC TGAAGTAGAT
    AAACAGTCAT TGTTCCAAAA AGTAAAGGAA ATGATTATGA CTGAAATAAT TAAAAAAGAA
    AATAAAGTGA AAAAGAAATT AGAAGAAAAG GAAGCTGAGA GAAAAGAAGA AGTTTCCCTG
    AGTGAGAAGA CATCCCCTCC TCCTCCTCCC CCTCCGGAAC CAGAGAAAGA GAAGGAAGTC
    CACCTTCCAC AGGAGCGGTC CAAAACTCCT GCTGCAAAAG GAAAGCAAGC ATCAGAACCC
    CTGCATGGTA ATCGAGAACC TCTTCAGGAA GGAAAAGGGA AGAAAAGTGA AACTTCACTC
    AAAAGAAAAG GTTCTCCTAG AGGAAAATCG CTAGGAGGGA AGATTCCACT CAAGAAATCA
    CCTGCCGAGT CTACAGATAT TTCACCGGTC CCGGTAGTGC CAGCCCCATC GAAGCCCGGA
    TCAGAAGAGT GGGTGTATGT AAATGAACCA ATTCCTGAGG AAATACCATC ATTTTTAGTA
    CCATACTGGA AATTGATAGA AAATTCCTAT ATAAATTCCA TCAAAACAGT ACTCAGGGAG
    TTGAGAGAAG GCCAGCATAG TGTACTTGCT TACTTATATG ATATTAGAAC AGGCTTCCAG
    CAGTTTCTGA AGCGTCCAGA CCACAAGCAA GACTTCGTGT CTCAGTGGCA GGCGGATTTC
    AACTCCCTTC CTGATGACCT GTGGGATGAT GAGGAAACAA AGGCTGAGCT GCACCAAAGA
    GTGAATGATT TACGGGACCG CCTGTGGGAC ATTTGCGATG CCCGTAAGGA AGAAGCAGAG
    CAGGAGCGTC TGGACATCAT TAACGAGAGC TGGTTGCAGG ACTCAATGGG AATTGCCATG
    AACCATTTCT TCTCACTGAT GCAGGCAGAA CTGAACCGTT TCCAAGATAC GAAGAGATTC
    CTGCAAGATT ATTACAGGGC CATGGAATGT AAAATTCCGA TGGATGACAC TAAGAAATTC
    ACTCGAGTCC CACTGGTCCG ACTGGATAGT AGAGATATTT TGGAAAGCCA GCTTAGAATT
    CCCCTGGTTC CTCGAAGATC CATTTCTCCA GAGTCAGCTT TATCCAAACC AAAAACAAAA
    AGCATCCTGA AGGGCAAGAT CGATTACACG CTGGAAAGTG TAGAGTTAAA CTTTGAGACT
    GATGAGAAGA TGGTGATGGA TACGTGGCAG CAGTCTTCTT TGGCAGTATC TCACATGGTG
    GCTGCTGAAA TTCATCAGAG GCTCATGGAA GAAGAAAAAG AAAACCAGCC AGCAGAAACA
    AAAGAAAAAC TTCCTCAGGC AGCTGCAAAT AAAAAAGTGA AAAAGGAGCC TCCGCCACCG
    CCACCACCAC CCAAGAAAAA GAAAGAGGAC AAAAAAGGAA AAGGTAAATC CCCACCTATG
    GCAGAAGCTA CTCCGGTAGT AGTGCCAGCA GAAGAGACTG AGGAAGTGGA ATTAAAAAAT
    GAACTGAAGC TCAGAATAAA GGAAGAGCAT CTTGCTGCCC TGCAATTTGA AGAGATAGCT
    ACACAGTTCC GACTTGAACT GATAAAGGTA AAAGCATTGG CTTTCCTGGA AGAGTTAGTA
    ATAAAGGCGG TTGATGTATA TAGACTCATG GACAAGTGGC TCGGTGAGAG GTACTTGAAT
    GAAATGGCCA GTGTGGAAAA ACTAACTGGG GTAGCTCGCT ATCACATTGA AACATCAACC
    AAAATTCAGA ATGAACTTTA TTTAGACCAA GAAGACTTCT TCATTAATGG CGATATCAAA
    GTCTTTCCAG ATCCACCTCC TCCGGTACGC CCTCCGCCTG TGGAAAAGGA GGAAAATGGC
    ACCCTGACCA TGGAGCAGCT TGACAACCTC CGGGATCAGT TCTTAGATAT AGCGCCCAAA
    GGTATAATAG GAAATAAAGC ATTTGCTGAC ATTCTGCTGG ATTTGGTAAC TCTGAACCTT
    GGAACAAACA ACTTTCCTAC TAGTTGGATG AACCTCACTC AACCGGAATT GCAGGAATTA
    GCATCTTTGT TGGTAACAAA CTCGGAGTCC GTGGACTGGC GGAAATTCTT GTTAGTCGTA
    GCGCTGCCTT GGCCCATCCC CCTGGAGGAG GAGCTTCTGG AGACCCTGCA GAGGTTCAAG
    GCCCTGGATG CAGAGCAGCT GGGCACCGTC ACTTATGAAC AGTATAAGCA GGCTGGCCTG
    TGGTTTACAG GAGATGAAGA TATCAAAATT CCAGAAAATC CTCTTGAACC CCTGCCATTC
    AATAGGCAGG AGCATCTTAT AGAGTTTTTC TTTAGGTTGT TTGCTGACTG CGAGAAGGTT
    CCACCCCAGC TTGACTACAC ACAGATGCTG CTTTATTTTG CTTGCCATCC AGACAGCACA
    GAAGGGGTCT ACAGGGCCCT CAGCGTAGCC ATTGGGACTC ACGTCTTCCA GCCAATTGAA
    ATGCCTCATC CTGTTGCAGC AAAGACCTCT TCCTTTACTG ATATGAGTCC CATTGAGGAA
    TATCCTGAAC TCGAGGACAA CTTTATGAGT GAGGAAAGGG AATTACAAGA GGACGGGGAG
    GAAAAGGAAG AAGAAATCCC TGAAAATGCA AATACGGAAA TGATTTCCAT GCAAACATTA
    CTCCAAGTGT TCCGAGGAGG AAATGAAGTA CTGGACGCTA ACAGATTTGC CAGCCACCAA
    AAGATAGAGA ACATTTATTT AGAGAACTTC ATAAAAGTGT TTCAGGACCT AGGTTCCAAG
    AACCTTGAGC CAATTGAAGT TGCTGTTCTT CTGAAGCATC CTTTTATTCA AGACCTGATT
    TCAAGTTATC CAGACTATAA GATCCCTGAT GTTAAGATAA TTCTCCAAAG GAGTGAGCAT
    GTACAAGGAA GTGATGAAGA GAGGTCTCCT TCAAGACTTA CAGAGGAAAA GAAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011985250.1
    CDS493..5769
    Translation

    Target ORF information:

    RefSeq Version XM_012129860.2
    Organism Ovis aries musimon(Ovis orientalis musimon)
    Definition Ovis aries musimon sperm flagellar 2 (SPEF2), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012129860.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGTCGGAAA TCCTGTGCCA GTGGCTCAAC CAGGAATTGA AGGTGTCCCA GACCGTGAGT 
    CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAA TTGGAGAAGT TTTGCACAAG
    TTTGCACTTC AGGATGATTT TGCAGACTTT TCAGAAAGCA GGATTTCCAG TGCCAGACTT
    AACAATTTTT CTCGCTTGGA GCCCACACTT CACCTTTTGG GTGTGGAATT TGATCAGAAT
    GTGGCCCACA ATATCATCAC AGAGAAGCCT GGGGCTGCCA CGAAACTCTT ATATCAGTTG
    TACATTGCTC TCCAGAGGAA AAAGAAAGGT GTTCAGACTA GAGTGGAAAT GCCAACTGTA
    CAGCCCCTGA TGAGCACAAA AGTTCAAAAC ATGAAAAGCG AGGCTTTCCG AGATCAACGC
    TTGAGCAAAA GACGACAAAA TGAAATCATG GCCAAAATCC AAGCAGCTAT CATACAGATT
    CCTAAACCCA CATCAAATCG TACTCTGAAA ACATTTGACG CCCAAAAATT AATGAAAAAG
    AAAAAAGAGG CAGAGGATGT GGCCAATGAA ATTAAGAAGT TTGAAGCATT AATAAAAAAG
    GATCTCCAGG CAAAAGAAAG TGCATCCAAG ACTTCTCTAG ATACAGCAGG CCAGACAACC
    GCTGATTTGT TAAACACTTA TTCAGATGAT GACTACATTA AAAAAATCCA AAAACGCCTT
    GAAGAAGATG CTTTTGCACG AGAGCAAAGG GAGAAAAGAC GGCGGAGACT CTTAATGGAT
    CAGTTAATAG CTCATGAAGC CCAAGAGGAG GCTTATCGAG AGGAGCAGTT GATCAACCGG
    CTGATGCGAC AGTCCCAGCA GGAACGCAGG ATCGCTGTGC AGCTCATGCA CGTTAGGCAC
    GAAAAGGAAG TTTTATGGCA AAACAGAATT TTCAGAGAAA AACAATATGA GGAAAGACGG
    CTTAAAGACT TCCAGGATGC TCTTGATCGA GAAGCGGCTT TAGCCAGACA AGCCAAGATT
    GATTTTGAAG AACAAGCCCT CAGAGATAAG GAAATTCATG AGCAGATCGC CATAGAAAGA
    GCTCAAGCTC GTTACAAAAA GCATTATTCA ATATGTGCAG AAATTTTGGA TCAGATACTT
    GATCTGTCCA CTAAGGTGGC AGACTATCGA TTGTTGACAA ATAACCTGAT CCCATATAAG
    TTGATGCATG ATTGGAAGGA ACTGTTTTTC CATGGAAAAC CCATATATGA GCAAGCCTCT
    CTTAGACATT TACCAGCTAA GCTCTCTGCA GAACACCTTG TAGAAATGGA GAAAAGGAAC
    TTGCTAGATA ACAATGATTA TGAAGAATAT AAGAACATGG TTGGAGAATG GGCCTTACCA
    GAAGAAATGG TTGAAAACTT ACCACCCTCC AACAATTGCA TATTGGGCCA TGTGATTCAC
    AGACTAATTG AAAGTTCTCT TCCTCCTCAA GTTGAATCTA CAGTACCTGA GTTACCTTCA
    TTTGCTATAA AAGGATGCTT GTTGGGGAAA ACCTTTAGTG GAAAAACTAC TGCTCTAAAG
    GCTCTAGAGA AAAGCTTTCC TATTCAGGTA CTTTCTATTG ACACTCTTGT TCAAGAAGCT
    ATCCAAGCAT TTCATGACAA TGAAAGTATC AGCGGGGCCC ATGGACTTCA GAAAGCACCT
    GAAGAAAAGG TTTTACCAGT TAGGCGAAAG AGTGGTAAAG AAAACCAGGA CGTGCAAGAT
    GAAGCACTAC CAGAAAGAGA AGATAAAACC AGACTTGATA CCGATGCTAG AAAAGCAGCA
    AAAGCTGAAG AAGTCGTAGC GAGTGATAGT TTCTCTGAAC TCACTGTGCG TGCTCAGCTT
    GGTGCAAAAT CAGAAATGCT GCTGAAGAAA GGAAAGAGCA TATCTGATAT ATTGCTAGTC
    AGCATCATGG TAAATGCCAT TAATCAGATA CCTATGTATA AAGGCTGGAT CCTGGATGGC
    TTTCCAATGA CATTAAACCA AGCAAAACTT CTGGAGGAAG CACTCACAGG CTACAACAGA
    AACCTCATAG AACTGGAGGG AAAGAAATCA CAAACATCCA CATTAGCTGT TGACCCTACA
    GCTTCCAAAG AAGTGCCTCT TCCCCCTTCT GCATTTGATT TTGTCATGTT ATTAGACATT
    TCAGATAATT CCTCACTGAA TCGCATGAAT GACATCATGG CTAAAGCATT TTCAAGTGAA
    GCTCCTCGTG AAGATATCAA TCAGCGTGTA GCTGCTAAAA ACAAAGATAC AGATGAGGAC
    CAGAATTTAA GAGATCAAAT ACAGCACAGA ATTGTAGGCT TCCTGGACAA CTGGCCTTCG
    TTGGAGCAGT GGTTTACAGA GCCAGAAAAC ATTTTGATAA AAATCAATGC TGAAGTAGAT
    AAACAGTCAT TGTTCCAAAA AGTAAAGGAA ATGATTATGA CTGAAATAAT TAAAAAAGAA
    AATAAAGTGA AAAAGAAATT AGAAGAAAAG GAAGCTGAGA GAAAAGAAGA AGTTTCCCTG
    AGTGAGAAGA CATCCCCTCC TCCTCCTCCC CCTCCGGAAC CAGAGAAAGA GAAGGAAGTC
    CACCTTCCAC AGGAGCGGTC CAAAACTCCT GCTGCAAAAG GAAAGCAAGC ATCAGAACCC
    CTGCATGGTA ATCGAGAACC TCTTCAGGAA GGAAAAGGGA AGAAAAGTGA AACTTCACTC
    AAAAGAAAAG GTTCTCCTAG AGGAAAATCG CTAGGAGGGA AGATTCCACT CAAGAAATCA
    CCTGCCGAGT CTACAGATAT TTCACCGGTC CCGGTAGTGC CAGCCCCATC GAAGCCCGGA
    TCAGAAGAGT GGGTGTATGT AAATGAACCA ATTCCTGAGG AAATACCATC ATTTTTAGTA
    CCATACTGGA AATTGATAGA AAATTCCTAT ATAAATTCCA TCAAAACAGT ACTCAGGGAG
    TTGAGAGAAG GCCAGCATAG TGTACTTGCT TACTTATATG ATATTAGAAC AGGCTTCCAG
    CAGTTTCTGA AGCGTCCAGA CCACAAGCAA GACTTCGTGT CTCAGTGGCA GGCGGATTTC
    AACTCCCTTC CTGATGACCT GTGGGATGAT GAGGAAACAA AGGCTGAGCT GCACCAAAGA
    GTGAATGATT TACGGGACCG CCTGTGGGAC ATTTGCGATG CCCGTAAGGA AGAAGCAGAG
    CAGGAGCGTC TGGACATCAT TAACGAGAGC TGGTTGCAGG ACTCAATGGG AATTGCCATG
    AACCATTTCT TCTCACTGAT GCAGGCAGAA CTGAACCGTT TCCAAGATAC GAAGAGATTC
    CTGCAAGATT ATTACAGGGC CATGGAATGT AAAATTCCGA TGGATGACAC TAAGAAATTC
    ACTCGAGTCC CACTGGTCCG ACTGGATAGT AGAGATATTT TGGAAAGCCA GCTTAGAATT
    CCCCTGGTTC CTCGAAGATC CATTTCTCCA GAGTCAGCTT TATCCAAACC AAAAACAAAA
    AGCATCCTGA AGGGCAAGAT CGATTACACG CTGGAAAGTG TAGAGTTAAA CTTTGAGACT
    GATGAGAAGA TGGTGATGGA TACGTGGCAG CAGTCTTCTT TGGCAGTATC TCACATGGTG
    GCTGCTGAAA TTCATCAGAG GCTCATGGAA GAAGAAAAAG AAAACCAGCC AGCAGAAACA
    AAAGAAAAAC TTCCTCAGGC AGCTGCAAAT AAAAAAGTGA AAAAGGAGCC TCCGCCACCG
    CCACCACCAC CCAAGAAAAA GAAAGAGGAC AAAAAAGGAA AAGGTAAATC CCCACCTATG
    GCAGAAGCTA CTCCGGTAGT AGTGCCAGCA GAAGAGACTG AGGAAGTGGA ATTAAAAAAT
    GAACTGAAGC TCAGAATAAA GGAAGAGCAT CTTGCTGCCC TGCAATTTGA AGAGATAGCT
    ACACAGTTCC GACTTGAACT GATAAAGGTA AAAGCATTGG CTTTCCTGGA AGAGTTAGTA
    ATAAAGGCGG TTGATGTATA TAGACTCATG GACAAGTGGC TCGGTGAGAG GTACTTGAAT
    GAAATGGCCA GTGTGGAAAA ACTAACTGGG GTAGCTCGCT ATCACATTGA AACATCAACC
    AAAATTCAGA ATGAACTTTA TTTAGACCAA GAAGACTTCT TCATTAATGG CGATATCAAA
    GTCTTTCCAG ATCCACCTCC TCCGGTACGC CCTCCGCCTG TGGAAAAGGA GGAAAATGGC
    ACCCTGACCA TGGAGCAGCT TGACAACCTC CGGGATCAGT TCTTAGATAT AGCGCCCAAA
    GGTATAATAG GAAATAAAGC ATTTGCTGAC ATTCTGCTGG ATTTGGTAAC TCTGAACCTT
    GGAACAAACA ACTTTCCTAC TAGTTGGATG AACCTCACTC AACCGGAATT GCAGGAATTA
    GCATCTTTGT TGGTAACAAA CTCGGAGTCC GTGGACTGGC GGAAATTCTT GTTAGTCGTA
    GCGCTGCCTT GGCCCATCCC CCTGGAGGAG GAGCTTCTGG AGACCCTGCA GAGGTTCAAG
    GCCCTGGATG CAGAGCAGCT GGGCACCGTC ACTTATGAAC AGTATAAGCA GGCTGGCCTG
    TGGTTTACAG GAGATGAAGA TATCAAAATT CCAGAAAATC CTCTTGAACC CCTGCCATTC
    AATAGGCAGG AGCATCTTAT AGAGTTTTTC TTTAGGTTGT TTGCTGACTG CGAGAAGGTT
    CCACCCCAGC TTGACTACAC ACAGATGCTG CTTTATTTTG CTTGCCATCC AGACAGCACA
    GAAGGGGTCT ACAGGGCCCT CAGCGTAGCC ATTGGGACTC ACGTCTTCCA GCCAATTGAA
    ATGCCTCATC CTGTTGCAGC AAAGACCTCT TCCTTTACTG ATATGAGTCC CATTGAGGAA
    TATCCTGAAC TCGAGGACAA CTTTATGAGT GAGGAAAGGG AATTACAAGA GGACGGGGAG
    GAAAAGGAAG AAGAAATCCC TGAAAATGCA AATACGGAAA TGATTTCCAT GCAAACATTA
    CTCCAAGTGT TCCGAGGAGG AAATGAAGTA CTGGACGCTA ACAGATTTGC CAGCCACCAA
    AAGATAGAGA ACATTTATTT AGAGAACTTC ATAAAAGTGT TTCAGGACCT AGGTTCCAAG
    AACCTTGAGC CAATTGAAGT TGCTGTTCTT CTGAAGCATC CTTTTATTCA AGACCTGATT
    TCAAGTTATC CAGACTATAA GATCCCTGAT GTTAAGATAA TTCTCCAAAG GAGTGAGCAT
    GTACAAGGAA GTGATGAAGA GAGGTCTCCT TCAAGACTTA CAGAGGAAAA GAAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OOv13490
    Clone ID Related Accession (Same CDS sequence) XM_012129862.2
    Accession Version XM_012129862.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5205bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-16
    Organism Ovis aries musimon(Ovis orientalis musimon)
    Product sperm flagellar protein 2 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942355.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012129862.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGTCGGAAA TCCTGTGCCA GTGGCTCAAC CAGGAATTGA AGGTGTCCCA GACCGTGAGT 
    CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAA TTGGAGAAGT TTTGCACAAG
    TTTGCACTTC AGGATGATTT TGCAGACTTT TCAGAAAGCA GGATTTCCAG TGCCAGACTT
    AACAATTTTT CTCGCTTGGA GCCCACACTT CACCTTTTGG GTGTGGAATT TGATCAGAAT
    GTGGCCCACA ATATCATCAC AGAGAAGCCT GGGGCTGCCA CGAAACTCTT ATATCAGTTG
    TACATTGCTC TCCAGAGGAA AAAGAAAGGT GTTCAGACTA GAGTGGAAAT GCCAACTGTA
    CAGCCCCTGA TGAGCACAAA AGTTCAAAAC ATGAAAAGCG AGGCTTTCCG AGATCAACGC
    TTGAGCAAAA GACGACAAAA TGAAATCATG GCCAAAATCC AAGCAGCTAT CATACAGATT
    CCTAAACCCA CATCAAATCG TACTCTGAAA ACATTTGACG CCCAAAAATT AATGAAAAAG
    AAAAAAGAGG CAGAGGATGT GGCCAATGAA ATTAAGAAGT TTGAAGCATT AATAAAAAAG
    GATCTCCAGG CAAAAGAAAG TGCATCCAAG ACTTCTCTAG ATACAGCAGG CCAGACAACC
    GCTGATTTGT TAAACACTTA TTCAGATGAT GACTACATTA AAAAAATCCA AAAACGCCTT
    GAAGAAGATG CTTTTGCACG AGAGCAAAGG GAGAAAAGAC GGCGGAGACT CTTAATGGAT
    CAGTTAATAG CTCATGAAGC CCAAGAGGAG GCTTATCGAG AGGAGCAGTT GATCAACCGG
    CTGATGCGAC AGTCCCAGCA GGAACGCAGG ATCGCTGTGC AGCTCATGCA CGTTAGGCAC
    GAAAAGGAAG TTTTATGGCA AAACAGAATT TTCAGAGAAA AACAATATGA GGAAAGACGG
    CTTAAAGACT TCCAGGATGC TCTTGATCGA GAAGCGGCTT TAGCCAGACA AGCCAAGATT
    GATTTTGAAG AACAAGCCCT CAGAGATAAG GAAATTCATG AGCAGATCGC CATAGAAAGA
    GCTCAAGCTC GTTACAAAAA GCATTATTCA ATATGTGCAG AAATTTTGGA TCAGATACTT
    GATCTGTCCA CTAAGGTGGC AGACTATCGA TTGTTGACAA ATAACCTGAT CCCATATAAG
    TTGATGCATG ATTGGAAGGA ACTGTTTTTC CATGGAAAAC CCATATATGA GCAAGCCTCT
    CTTAGACATT TACCAGCTAA GCTCTCTGCA GAACACCTTG TAGAAATGGA GAAAAGGAAC
    TTGCTAGATA ACAATGATTA TGAAGAATAT AAGAACATGG TTGGAGAATG GGCCTTACCA
    GAAGAAATGG TTGAAAACTT ACCACCCTCC AACAATTGCA TATTGGGCCA TGTGATTCAC
    AGACTAATTG AAAGTTCTCT TCCTCCTCAA GTTGAATCTA CAGTACCTGA GTTACCTTCA
    TTTGCTATAA AAGGATGCTT GTTGGGGAAA ACCTTTAGTG GAAAAACTAC TGCTCTAAAG
    GCTCTAGAGA AAAGCTTTCC TATTCAGGTA CTTTCTATTG ACACTCTTGT TCAAGAAGCT
    ATCCAAGCAT TTCATGACAA TGAAAGTATC AGCGGGGCCC ATGGACTTCA GAAAGCACCT
    GAAGAAAAGG TTTTACCAGT TAGGCGAAAG AGTGGTAAAG AAAACCAGGA CGTGCAAGAT
    GAAGCACTAC CAGAAAGAGA AGATAAAACC AGACTTGATA CCGATGCTAG AAAAGCAGCA
    AAAGCTGAAG AAGTCGTAGC GAGTGATAGT TTCTCTGAAC TCACTGTGCG TGCTCAGCTT
    GGTGCAAAAT CAGAAATGCT GCTGAAGAAA GGAAAGAGCA TATCTGATAT ATTGCTAGTC
    AGCATCATGG TAAATGCCAT TAATCAGATA CCTATGTATA AAGGCTGGAT CCTGGATGGC
    TTTCCAATGA CATTAAACCA AGCAAAACTT CTGGAGGAAG CACTCACAGG CTACAACAGA
    AACCTCATAG AACTGGAGGG AAAGAAATCA CAAACATCCA CATTAGCTGT TGACCCTACA
    GCTTCCAAAG AAGTGCCTCT TCCCCCTTCT GCATTTGATT TTGTCATGTT ATTAGACATT
    TCAGATAATT CCTCACTGAA TCGCATGAAT GACATCATGG CTAAAGCATT TTCAAGTGAA
    GCTCCTCGTG AAGATATCAA TCAGCGTGTA GCTGCTAAAA ACAAAGATAC AGATGAGGAC
    CAGAATTTAA GAGATCAAAT ACAGCACAGA ATTGTAGGCT TCCTGGACAA CTGGCCTTCG
    TTGGAGCAGT GGTTTACAGA GCCAGAAAAC ATTTTGATAA AAATCAATGC TGAAGTAGAT
    AAACAGTCAT TGTTCCAAAA AGTAAAGGAA ATGATTATGA CTGAAATAAT TAAAAAAGAA
    AATAAAGTGA AAAAGAAATT AGAAGAAAAG GAAGCTGAGA GAAAAGAAGA AGTTTCCCTG
    AGTGAGAAGA CATCCCCTCC TCCTCCTCCC CCTCCGGAAC CAGAGAAAGA GAAGGAAGTC
    CACCTTCCAC AGGAGCGGTC CAAAACTCCT GCTGCAAAAG GAAAGCAAGC ATCAGAACCC
    CTGCATGGTA ATCGAGAACC TCTTCAGGAA GGAAAAGGGA AGAAAAGTGA AACTTCACTC
    AAAAGAAAAG GTTCTCCTAG AGGAAAATCG CTAGGAGGGA AGATTCCACT CAAGAAATCA
    CCTGCCGAGT CTACAGATAT TTCACCGGTC CCGGTAGTGC CAGCCCCATC GAAGCCCGGA
    TCAGAAGAGT GGGTGTATGT AAATGAACCA ATTCCTGAGG AAATACCATC ATTTTTAGTA
    CCATACTGGA AATTGATAGA AAATTCCTAT ATAAATTCCA TCAAAACAGT ACTCAGGGAG
    TTGAGAGAAG GCCAGCATAG TGTACTTGCT TACTTATATG ATATTAGAAC AGGCTTCCAG
    CAGTTTCTGA AGCGTCCAGA CCACAAGCAA GACTTCGTGT CTCAGTGGCA GGCGGATTTC
    AACTCCCTTC CTGATGACCT GTGGGATGAT GAGGAAACAA AGGCTGAGCT GCACCAAAGA
    GTGAATGATT TACGGGACCG CCTGTGGGAC ATTTGCGATG CCCGTAAGGA AGAAGCAGAG
    CAGGAGCGTC TGGACATCAT TAACGAGAGC TGGTTGCAGG ACTCAATGGG AATTGCCATG
    AACCATTTCT TCTCACTGAT GCAGGCAGAA CTGAACCGTT TCCAAGATAC GAAGAGATTC
    CTGCAAGATT ATTACAGGGC CATGGAATGT AAAATTCCGA TGGATGACAC TAAGAAATTC
    ACTCGAGTCC CACTGGTCCG ACTGGATAGT AGAGATATTT TGGAAAGCCA GCTTAGAATT
    CCCCTGGTTC CTCGAAGATC CATTTCTCCA GAGTCAGCTT TATCCAAACC AAAAACAAAA
    AGCATCCTGA AGGGCAAGAT CGATTACACG CTGGAAAGTG TAGAGTTAAA CTTTGAGACT
    GATGAGAAGA TGGTGATGGA TACGTGGCAG CAGTCTTCTT TGGCAGTATC TCACATGGTG
    GCTGCTGAAA TTCATCAGAG GCTCATGGAA GAAGAAAAAG AAAACCAGCC AGCAGAAACA
    AAAGAAAAAC TTCCTCAGGC AGCTGCAAAT AAAAAAGTGA AAAAGGAGCC TCCGCCACCG
    CCACCACCAC CCAAGAAAAA GAAAGAGGAC AAAAAAGGAA AAGGTAAATC CCCACCTATG
    GCAGAAGCTA CTCCGGTAGT AGTGCCAGCA GAAGAGACTG AGGAAGTGGA ATTAAAAAAT
    GAACTGAAGC TCAGAATAAA GGAAGAGCAT CTTGCTGCCC TGCAATTTGA AGAGATAGCT
    ACACAGTTCC GACTTGAACT GATAAAGGTA AAAGCATTGG CTTTCCTGGA AGAGTTAGTA
    ATAAAGGCGG TTGATGTATA TAGACTCATG GACAAGTGGC TCGGTGAGAG GTACTTGAAT
    GAAATGGCCA GTGTGGAAAA ACTAACTGGG GTAGCTCGCT ATCACATTGA AACATCAACC
    AAAATTCAGA ATGAACTTTA TTTAGACCAA GAAGACTTCT TCATTAATGG CGATATCAAA
    GTCTTTCCAG ATCCACCTCC TCCGGTACGC CCTCCGCCTG TGGAAAAGGA GGAAAATGGC
    ACCCTGACCA TGGAGCAGCT TGACAACCTC CGGGATCAGT TCTTAGATAT AGCGCCCAAA
    GGTATAATAG GAAATAAAGC ATTTGCTGAC ATTCTGCTGG ATTTGGTAAC TCTGAACCTT
    GGAACAAACA ACTTTCCTAC TAGTTGGATG AACCTCACTC AACCGGAATT GCAGGAATTA
    GCATCTTTGT TGGTAACAAA CTCGGAGTCC GTGGACTGGC GGAAATTCTT GTTAGTCGTA
    GCGCTGCCTT GGCCCATCCC CCTGGAGGAG GAGCTTCTGG AGACCCTGCA GAGGTTCAAG
    GCCCTGGATG CAGAGCAGCT GGGCACCGTC ACTTATGAAC AGTATAAGCA GGCTGGCCTG
    TGGTTTACAG GAGATGAAGA TATCAAAATT CCAGAAAATC CTCTTGAACC CCTGCCATTC
    AATAGGCAGG AGCATCTTAT AGAGTTTTTC TTTAGGTTGT TTGCTGACTG CGAGAAGGTT
    CCACCCCAGC TTGACTACAC ACAGATGCTG CTTTATTTTG CTTGCCATCC AGACAGCACA
    GAAGGGGTCT ACAGGGCCCT CAGCGTAGCC ATTGGGACTC ACGTCTTCCA GCCAATTGAA
    ATGCCTCATC CTGTTGCAGC AAAGACCTCT TCCTTTACTG ATATGAGTCC CATTGAGGAA
    TATCCTGAAC TCGAGGACAA CTTTATGAGT GAGGAAAGGG AATTACAAGA GGACGGGGAG
    GAAAAGGAAG AAGAAATCCC TGAAAATGCA AATACGGAAA TGATTTCCAT GCAAACATTA
    CTCCAAGTGT TCCGAGGAGG AAATGAAGTA CTGGACGCTA ACAGATTTGC CAGCCACCAA
    AAGATAGAGA ACATTTATTT AGAGAACTTC ATAAAAGTGT TTCAGGACCT AGGTTCCAAG
    AACCTTGAGC CAATTGAAGT TGCTGTTCTT CTGAAGCATC CTTTTATTCA AGACCTGATT
    TCAAGTTATC CAGACTATAA GATCCCTATA TCCATGTGCA AGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011985252.1
    CDS493..5697
    Translation

    Target ORF information:

    RefSeq Version XM_012129862.2
    Organism Ovis aries musimon(Ovis orientalis musimon)
    Definition Ovis aries musimon sperm flagellar 2 (SPEF2), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012129862.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGTCGGAAA TCCTGTGCCA GTGGCTCAAC CAGGAATTGA AGGTGTCCCA GACCGTGAGT 
    CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAA TTGGAGAAGT TTTGCACAAG
    TTTGCACTTC AGGATGATTT TGCAGACTTT TCAGAAAGCA GGATTTCCAG TGCCAGACTT
    AACAATTTTT CTCGCTTGGA GCCCACACTT CACCTTTTGG GTGTGGAATT TGATCAGAAT
    GTGGCCCACA ATATCATCAC AGAGAAGCCT GGGGCTGCCA CGAAACTCTT ATATCAGTTG
    TACATTGCTC TCCAGAGGAA AAAGAAAGGT GTTCAGACTA GAGTGGAAAT GCCAACTGTA
    CAGCCCCTGA TGAGCACAAA AGTTCAAAAC ATGAAAAGCG AGGCTTTCCG AGATCAACGC
    TTGAGCAAAA GACGACAAAA TGAAATCATG GCCAAAATCC AAGCAGCTAT CATACAGATT
    CCTAAACCCA CATCAAATCG TACTCTGAAA ACATTTGACG CCCAAAAATT AATGAAAAAG
    AAAAAAGAGG CAGAGGATGT GGCCAATGAA ATTAAGAAGT TTGAAGCATT AATAAAAAAG
    GATCTCCAGG CAAAAGAAAG TGCATCCAAG ACTTCTCTAG ATACAGCAGG CCAGACAACC
    GCTGATTTGT TAAACACTTA TTCAGATGAT GACTACATTA AAAAAATCCA AAAACGCCTT
    GAAGAAGATG CTTTTGCACG AGAGCAAAGG GAGAAAAGAC GGCGGAGACT CTTAATGGAT
    CAGTTAATAG CTCATGAAGC CCAAGAGGAG GCTTATCGAG AGGAGCAGTT GATCAACCGG
    CTGATGCGAC AGTCCCAGCA GGAACGCAGG ATCGCTGTGC AGCTCATGCA CGTTAGGCAC
    GAAAAGGAAG TTTTATGGCA AAACAGAATT TTCAGAGAAA AACAATATGA GGAAAGACGG
    CTTAAAGACT TCCAGGATGC TCTTGATCGA GAAGCGGCTT TAGCCAGACA AGCCAAGATT
    GATTTTGAAG AACAAGCCCT CAGAGATAAG GAAATTCATG AGCAGATCGC CATAGAAAGA
    GCTCAAGCTC GTTACAAAAA GCATTATTCA ATATGTGCAG AAATTTTGGA TCAGATACTT
    GATCTGTCCA CTAAGGTGGC AGACTATCGA TTGTTGACAA ATAACCTGAT CCCATATAAG
    TTGATGCATG ATTGGAAGGA ACTGTTTTTC CATGGAAAAC CCATATATGA GCAAGCCTCT
    CTTAGACATT TACCAGCTAA GCTCTCTGCA GAACACCTTG TAGAAATGGA GAAAAGGAAC
    TTGCTAGATA ACAATGATTA TGAAGAATAT AAGAACATGG TTGGAGAATG GGCCTTACCA
    GAAGAAATGG TTGAAAACTT ACCACCCTCC AACAATTGCA TATTGGGCCA TGTGATTCAC
    AGACTAATTG AAAGTTCTCT TCCTCCTCAA GTTGAATCTA CAGTACCTGA GTTACCTTCA
    TTTGCTATAA AAGGATGCTT GTTGGGGAAA ACCTTTAGTG GAAAAACTAC TGCTCTAAAG
    GCTCTAGAGA AAAGCTTTCC TATTCAGGTA CTTTCTATTG ACACTCTTGT TCAAGAAGCT
    ATCCAAGCAT TTCATGACAA TGAAAGTATC AGCGGGGCCC ATGGACTTCA GAAAGCACCT
    GAAGAAAAGG TTTTACCAGT TAGGCGAAAG AGTGGTAAAG AAAACCAGGA CGTGCAAGAT
    GAAGCACTAC CAGAAAGAGA AGATAAAACC AGACTTGATA CCGATGCTAG AAAAGCAGCA
    AAAGCTGAAG AAGTCGTAGC GAGTGATAGT TTCTCTGAAC TCACTGTGCG TGCTCAGCTT
    GGTGCAAAAT CAGAAATGCT GCTGAAGAAA GGAAAGAGCA TATCTGATAT ATTGCTAGTC
    AGCATCATGG TAAATGCCAT TAATCAGATA CCTATGTATA AAGGCTGGAT CCTGGATGGC
    TTTCCAATGA CATTAAACCA AGCAAAACTT CTGGAGGAAG CACTCACAGG CTACAACAGA
    AACCTCATAG AACTGGAGGG AAAGAAATCA CAAACATCCA CATTAGCTGT TGACCCTACA
    GCTTCCAAAG AAGTGCCTCT TCCCCCTTCT GCATTTGATT TTGTCATGTT ATTAGACATT
    TCAGATAATT CCTCACTGAA TCGCATGAAT GACATCATGG CTAAAGCATT TTCAAGTGAA
    GCTCCTCGTG AAGATATCAA TCAGCGTGTA GCTGCTAAAA ACAAAGATAC AGATGAGGAC
    CAGAATTTAA GAGATCAAAT ACAGCACAGA ATTGTAGGCT TCCTGGACAA CTGGCCTTCG
    TTGGAGCAGT GGTTTACAGA GCCAGAAAAC ATTTTGATAA AAATCAATGC TGAAGTAGAT
    AAACAGTCAT TGTTCCAAAA AGTAAAGGAA ATGATTATGA CTGAAATAAT TAAAAAAGAA
    AATAAAGTGA AAAAGAAATT AGAAGAAAAG GAAGCTGAGA GAAAAGAAGA AGTTTCCCTG
    AGTGAGAAGA CATCCCCTCC TCCTCCTCCC CCTCCGGAAC CAGAGAAAGA GAAGGAAGTC
    CACCTTCCAC AGGAGCGGTC CAAAACTCCT GCTGCAAAAG GAAAGCAAGC ATCAGAACCC
    CTGCATGGTA ATCGAGAACC TCTTCAGGAA GGAAAAGGGA AGAAAAGTGA AACTTCACTC
    AAAAGAAAAG GTTCTCCTAG AGGAAAATCG CTAGGAGGGA AGATTCCACT CAAGAAATCA
    CCTGCCGAGT CTACAGATAT TTCACCGGTC CCGGTAGTGC CAGCCCCATC GAAGCCCGGA
    TCAGAAGAGT GGGTGTATGT AAATGAACCA ATTCCTGAGG AAATACCATC ATTTTTAGTA
    CCATACTGGA AATTGATAGA AAATTCCTAT ATAAATTCCA TCAAAACAGT ACTCAGGGAG
    TTGAGAGAAG GCCAGCATAG TGTACTTGCT TACTTATATG ATATTAGAAC AGGCTTCCAG
    CAGTTTCTGA AGCGTCCAGA CCACAAGCAA GACTTCGTGT CTCAGTGGCA GGCGGATTTC
    AACTCCCTTC CTGATGACCT GTGGGATGAT GAGGAAACAA AGGCTGAGCT GCACCAAAGA
    GTGAATGATT TACGGGACCG CCTGTGGGAC ATTTGCGATG CCCGTAAGGA AGAAGCAGAG
    CAGGAGCGTC TGGACATCAT TAACGAGAGC TGGTTGCAGG ACTCAATGGG AATTGCCATG
    AACCATTTCT TCTCACTGAT GCAGGCAGAA CTGAACCGTT TCCAAGATAC GAAGAGATTC
    CTGCAAGATT ATTACAGGGC CATGGAATGT AAAATTCCGA TGGATGACAC TAAGAAATTC
    ACTCGAGTCC CACTGGTCCG ACTGGATAGT AGAGATATTT TGGAAAGCCA GCTTAGAATT
    CCCCTGGTTC CTCGAAGATC CATTTCTCCA GAGTCAGCTT TATCCAAACC AAAAACAAAA
    AGCATCCTGA AGGGCAAGAT CGATTACACG CTGGAAAGTG TAGAGTTAAA CTTTGAGACT
    GATGAGAAGA TGGTGATGGA TACGTGGCAG CAGTCTTCTT TGGCAGTATC TCACATGGTG
    GCTGCTGAAA TTCATCAGAG GCTCATGGAA GAAGAAAAAG AAAACCAGCC AGCAGAAACA
    AAAGAAAAAC TTCCTCAGGC AGCTGCAAAT AAAAAAGTGA AAAAGGAGCC TCCGCCACCG
    CCACCACCAC CCAAGAAAAA GAAAGAGGAC AAAAAAGGAA AAGGTAAATC CCCACCTATG
    GCAGAAGCTA CTCCGGTAGT AGTGCCAGCA GAAGAGACTG AGGAAGTGGA ATTAAAAAAT
    GAACTGAAGC TCAGAATAAA GGAAGAGCAT CTTGCTGCCC TGCAATTTGA AGAGATAGCT
    ACACAGTTCC GACTTGAACT GATAAAGGTA AAAGCATTGG CTTTCCTGGA AGAGTTAGTA
    ATAAAGGCGG TTGATGTATA TAGACTCATG GACAAGTGGC TCGGTGAGAG GTACTTGAAT
    GAAATGGCCA GTGTGGAAAA ACTAACTGGG GTAGCTCGCT ATCACATTGA AACATCAACC
    AAAATTCAGA ATGAACTTTA TTTAGACCAA GAAGACTTCT TCATTAATGG CGATATCAAA
    GTCTTTCCAG ATCCACCTCC TCCGGTACGC CCTCCGCCTG TGGAAAAGGA GGAAAATGGC
    ACCCTGACCA TGGAGCAGCT TGACAACCTC CGGGATCAGT TCTTAGATAT AGCGCCCAAA
    GGTATAATAG GAAATAAAGC ATTTGCTGAC ATTCTGCTGG ATTTGGTAAC TCTGAACCTT
    GGAACAAACA ACTTTCCTAC TAGTTGGATG AACCTCACTC AACCGGAATT GCAGGAATTA
    GCATCTTTGT TGGTAACAAA CTCGGAGTCC GTGGACTGGC GGAAATTCTT GTTAGTCGTA
    GCGCTGCCTT GGCCCATCCC CCTGGAGGAG GAGCTTCTGG AGACCCTGCA GAGGTTCAAG
    GCCCTGGATG CAGAGCAGCT GGGCACCGTC ACTTATGAAC AGTATAAGCA GGCTGGCCTG
    TGGTTTACAG GAGATGAAGA TATCAAAATT CCAGAAAATC CTCTTGAACC CCTGCCATTC
    AATAGGCAGG AGCATCTTAT AGAGTTTTTC TTTAGGTTGT TTGCTGACTG CGAGAAGGTT
    CCACCCCAGC TTGACTACAC ACAGATGCTG CTTTATTTTG CTTGCCATCC AGACAGCACA
    GAAGGGGTCT ACAGGGCCCT CAGCGTAGCC ATTGGGACTC ACGTCTTCCA GCCAATTGAA
    ATGCCTCATC CTGTTGCAGC AAAGACCTCT TCCTTTACTG ATATGAGTCC CATTGAGGAA
    TATCCTGAAC TCGAGGACAA CTTTATGAGT GAGGAAAGGG AATTACAAGA GGACGGGGAG
    GAAAAGGAAG AAGAAATCCC TGAAAATGCA AATACGGAAA TGATTTCCAT GCAAACATTA
    CTCCAAGTGT TCCGAGGAGG AAATGAAGTA CTGGACGCTA ACAGATTTGC CAGCCACCAA
    AAGATAGAGA ACATTTATTT AGAGAACTTC ATAAAAGTGT TTCAGGACCT AGGTTCCAAG
    AACCTTGAGC CAATTGAAGT TGCTGTTCTT CTGAAGCATC CTTTTATTCA AGACCTGATT
    TCAAGTTATC CAGACTATAA GATCCCTATA TCCATGTGCA AGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OOv13491
    Clone ID Related Accession (Same CDS sequence) XM_015106494.1
    Accession Version XM_015106494.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5202bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-16
    Organism Ovis aries musimon(Ovis orientalis musimon)
    Product sperm flagellar protein 2 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942355.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGAGGAAAG ACGGCTTAAA GACTTCCAGG ATGCTCTTGA TCGAGAAGCG CCTGATCCCA 
    TATAAGTTGA TGCATGATTG GAAGGAACTG TTTTTCCATG GAAAACCCAT ATATGAGCAA
    GCCTCTCTTA GACATTTACC AGCTAAGCTC TCTGCAGAAC ACCTTGTAGA AATGGAGAAA
    AGGAACTTGC TAGATAACAA TGATTATGAA GAATATAAGA ACATGGTTGG AGAATGGGCC
    TTACCAGAAG AAATGGTTGA AAACTTACCA CCCTCCAACA ATTGCATATT GGGCCATGTG
    ATTCACAGAC TAATTGAAAG TTCTCTTCCT CCTCAAGTTG AATCTACAGT ACCTGAGTTA
    CCTTCATTTG CTATAAAAGG ATGCTTGTTG GGGAAAACCT TTAGTGGAAA AACTACTGCT
    CTAAAGGCTC TAGAGAAAAG CTTTCCTATT CAGGTACTTT CTATTGACAC TCTTGTTCAA
    GAAGCTATCC AAGCATTTCA TGACAATGAA AGTATCAGCG GGGCCCATGG ACTTCAGAAA
    GCACCTGAAG AAAAGGTTTT ACCAGTTAGG CGAAAGAGTG GTAAAGAAAA CCAGGACGTG
    CAAGATGAAG CACTACCAGA AAGAGAAGAT AAAACCAGAC TTGATACCGA TGCTAGAAAA
    GCAGCAAAAG CTGAAGAAGT CGTAGCGAGT GATAGTTTCT CTGAACTCAC TGTGCGTGCT
    CAGCTTGGTG CAAAATCAGA AATGCTGCTG AAGAAAGGAA AGAGCATATC TGATATATTG
    CTAGTCAGCA TCATGGTAAA TGCCATTAAT CAGATACCTA TGTATAAAGG CTGGATCCTG
    GATGGCTTTC CAATGACATT AAACCAAGCA AAACTTCTGG AGGAAGCACT CACAGGCTAC
    AACAGAAACC TCATAGAACT GGAGGGAAAG AAATCACAAA CATCCACATT AGCTGTTGAC
    CCTACAGCTT CCAAAGAAGT GCCTCTTCCC CCTTCTGCAT TTGATTTTGT CATGTTATTA
    GACATTTCAG ATAATTCCTC ACTGAATCGC ATGAATGACA TCATGGCTAA AGCATTTTCA
    AGTGAAGCTC CTCGTGAAGA TATCAATCAG CGTGTAGCTG CTAAAAACAA AGATACAGAT
    GAGGACCAGA ATTTAAGAGA TCAAATACAG CACAGAATTG TAGGCTTCCT GGACAACTGG
    CCTTCGTTGG AGCAGTGGTT TACAGAGCCA GAAAACATTT TGATAAAAAT CAATGCTGAA
    GTAGATAAAC AGTCATTGTT CCAAAAAGTA AAGGAAATGA TTATGACTGA AATAATTAAA
    AAAGAAAATA AAGTGAAAAA GAAATTAGAA GAAAAGGAAG CTGAGAGAAA AGAAGAAGTT
    TCCCTGAGTG AGAAGACATC CCCTCCTCCT CCTCCCCCTC CGGAACCAGA GAAAGAGAAG
    GAAGTCCACC TTCCACAGGA GCGGTCCAAA ACTCCTGCTG CAAAAGGAAA GCAAGCATCA
    GAACCCCTGC ATGGTAATCG AGAACCTCTT CAGGAAGGAA AAGGGAAGAA AAGTGAAACT
    TCACTCAAAA GAAAAGGTTC TCCTAGAGGA AAATCGCTAG GAGGGAAGAT TCCACTCAAG
    AAATCACCTG CCGAGTCTAC AGATATTTCA CCGGTCCCGG TAGTGCCAGC CCCATCGAAG
    CCCGGATCAG AAGAGTGGGT GTATGTAAAT GAACCAATTC CTGAGGAAAT ACCATCATTT
    TTAGTACCAT ACTGGAAATT GATAGAAAAT TCCTATATAA ATTCCATCAA AACAGTACTC
    AGGGAGTTGA GAGAAGGCCA GCATAGTGTA CTTGCTTACT TATATGATAT TAGAACAGGC
    TTCCAGCAGT TTCTGAAGCG TCCAGACCAC AAGCAAGACT TCGTGTCTCA GTGGCAGGCG
    GATTTCAACT CCCTTCCTGA TGACCTGTGG GATGATGAGG AAACAAAGGC TGAGCTGCAC
    CAAAGAGTGA ATGATTTACG GGACCGCCTG TGGGACATTT GCGATGCCCG TAAGGAAGAA
    GCAGAGCAGG AGCGTCTGGA CATCATTAAC GAGAGCTGGT TGCAGGACTC AATGGGAATT
    GCCATGAACC ATTTCTTCTC ACTGATGCAG GCAGAACTGA ACCGTTTCCA AGATACGAAG
    AGATTCCTGC AAGATTATTA CAGGGCCATG GAATGTAAAA TTCCGATGGA TGACACTAAG
    AAATTCACTC GAGTCCCACT GGTCCGACTG GATAGTAGAG ATATTTTGGA AAGCCAGCTT
    AGAATTCCCC TGGTTCCTCG AAGATCCATT TCTCCAGAGT CAGCTTTATC CAAACCAAAA
    ACAAAAAGCA TCCTGAAGGG CAAGATCGAT TACACGCTGG AAAGTGTAGA GTTAAACTTT
    GAGACTGATG AGAAGATGGT GATGGATACG TGGCAGCAGT CTTCTTTGGC AGTATCTCAC
    ATGGTGGCTG CTGAAATTCA TCAGAGGCTC ATGGAAGAAG AAAAAGAAAA CCAGCCAGCA
    GAAACAAAAG AAAAACTTCC TCAGGCAGCT GCAAATAAAA AAGTGAAAAA GGAGCCTCCG
    CCACCGCCAC CACCACCCAA GAAAAAGAAA GAGGACAAAA AAGGAAAAGG TAAATCCCCA
    CCTATGGCAG AAGCTACTCC GGTAGTAGTG CCAGCAGAAG AGACTGAGGA AGTGGAATTA
    AAAAATGAAC TGAAGCTCAG AATAAAGGAA GAGCATCTTG CTGCCCTGCA ATTTGAAGAG
    ATAGCTACAC AGTTCCGACT TGAACTGATA AAGGTAAAAG CATTGGCTTT CCTGGAAGAG
    TTAGTAATAA AGGCGGTTGA TGTATATAGA CTCATGGACA AGTGGCTCGG TGAGAGGTAC
    TTGAATGAAA TGGCCAGTGT GGAAAAACTA ACTGGGGTAG CTCGCTATCA CATTGAAACA
    TCAACCAAAA TTCAGAATGA ACTTTATTTA GACCAAGAAG ACTTCTTCAT TAATGGCGAT
    ATCAAAGTCT TTCCAGATCC ACCTCCTCCG GTACGCCCTC CGCCTGTGGA AAAGGAGGAA
    AATGGCACCC TGACCATGGA GCAGCTTGAC AACCTCCGGG ATCAGTTCTT AGATATAGCG
    CCCAAAGGCA GGGATCCTCC AGACAATGTG AGTGGATTCA CCAAGAAGCA GAAAAGGCCT
    CGGAAGCTTG ACCATTACCT CCCAAAGAGG AACAATCAAT TTTTGAAAAT TTGGCTTAAA
    AAATACCCTT GGCTTGTATA CGATGAGATA CTAAATCTTA TGTTCTGCGG CCTGTGTCGT
    AAACATGGAG TGACATTAAA GGGAAATCAG GTCAGTTTTT TTTATGGCAC GGATAACTTC
    AGGACTGAAT TTCTCAACGC GCATCATCTG AGTGAGGCTC ACGCCAAGGC GTCCCTCATG
    GAAGCCACGA GTGGTTCTCC AGGGAGCAGA GCTACCACGG AGCTGATGGT GAGGACCATG
    AGCAAGGTGA CCCTTGGCAG GGTGGAGAAC CTGTTCCGGT CCTGCCACGC CATCGCCAAG
    ACCGGACGTC CCCTCAAAGA CTTCCTCTGG ATGTGTTCGC TGGACGATAT GAAGGGAGTT
    GATATCGGCC CGGTGTTCAG AACCAGTAAA TCGGCCAGAA CTTTTACCTA CTTTATTGCT
    GAAGTGGAAC GGAGAACTCT AAGAGAGAAG TTAGAAAAAA GTCAGTTCTT CTCCGTCCTC
    AGTGATGGGA TTGCAGACAG TTCAGCTGAG GAAGCTGAAC TCGTATATGT GCAGTTTGCC
    CGCGCAGGGA GAGTGCACTG CCAAATCGTC GGGGTACAGA CCGTGGAGAA GAAGGAGCCC
    TGGGCAATAA AACGTGCCAT CGAGAAAACT CTGGAGAGAA ATCTTCAACT TCGACTGTCG
    AACCAAGACT GGGCAAAGAA ACTGGTGGGT TTCGGAAGCG ATGGAGCTCC AGGTTCAGAG
    GGAGAAAACG GCAGCACAGT GGCCCTGCTT TTAAGGGAAA TCCAGCCAGG TGTGCTGCCT
    GTTTACTGCC TTGCCCATCA CCTCGAACTG TCTTACCAGG CGGTGTTCCA GAGCGTTCCC
    CTCTACAGCC GTGTGAAAGA CCTTCTTCAT AGCATGCACC GCTTCTATCA CGATTCTCCT
    GCTCACAGGA GTGCTCTGGT GACTGCTTTC AAGGGCCTCC ACCTTCAACC TGCGATGCCC
    TCTCAAGTCA GAGGCCGGAG GTGGCTTCAG GGATTGCAGG CTGCCCTCCA GAACTTTCTC
    AAGGGATATC CAGCAATTGT CCAGCAGCTG CAGTCAACAG AAGAAAGCAG AAGCAACAGC
    AGTCATCAGA AAGCCGAAGA ACTCCTGGAG TCTCTCCTCC AGGCAGACAT TGTCAAATTT
    GTCCACTTCT TGGTGGATGT CGTCAATGTC CTTAGCATAC TGTCCCATGT CAATCAGAAC
    AGAAATTCCT CAATTGCTGA TGTATTTGCC ACCTTAGAGT CAACACTGGA AATGCTCCAA
    ATTTATCAGA CAAGACCAGG GCCGAAAGAA CGTGGGGTAG ATTCGGTCAC ACACTTTCAT
    GGTAACTGCC TTGGAGGAAA GGGAAACATT TCTGACGTGA GAAACATTGT TTTAACACAT
    CTTATCAAGA GGCTGCGAGG CTGTTTCAGA GATGCCAACC AAGATGTGGT GAAGGCAACC
    ACGGTTGGAA GCTTTAAACT GTGGCCGGCA AAAATAAATC AAGAATTTGG AGAAAAAGAG
    GTGGCCATCC TGATTGCACA TTATGAACCA GTATTAGAGG CAGCCAAGGT GAAAATTGAC
    CAAGTAGACA CAGAATGGAG CATGTTGAAA TTGGAGATCT ACTCTAGATT TCAGAACATT
    TGCAAACTGA CCTGGGATTT TGTGAATTCT GTTTATTTAC ACAAGTATCC AAACATCCTG
    ACACTCATTG ACCTTGTGCT GAGCCTGCCT GCAAGTTCAG CTGAGGCAGG ACGTGGCTTT
    AGTCAGATGA AGCGCATCAG GTCCCAAATG CATGCCAAGG TCAAGGCTGA CAACATGACG
    GATCTCCTGA TAATTCAGTT GAATTCTCCA GACATTAACA ACTTTGACCC TAGGAAAGCC
    ATTCATTTAT GGAATACAAG AACACCATCC TCAACTGAGG ACACAAGACC TAACTCAGAC
    TGCTCATCAA ACTCAGAAAG CCTTAGTGAA AGCGATAAGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_014961980.1
    CDS134..5335
    Translation

    Target ORF information:

    RefSeq Version XM_015106494.1
    Organism Ovis aries musimon(Ovis orientalis musimon)
    Definition Ovis aries musimon sperm flagellar 2 (SPEF2), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015106494.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGAGGAAAG ACGGCTTAAA GACTTCCAGG ATGCTCTTGA TCGAGAAGCG CCTGATCCCA 
    TATAAGTTGA TGCATGATTG GAAGGAACTG TTTTTCCATG GAAAACCCAT ATATGAGCAA
    GCCTCTCTTA GACATTTACC AGCTAAGCTC TCTGCAGAAC ACCTTGTAGA AATGGAGAAA
    AGGAACTTGC TAGATAACAA TGATTATGAA GAATATAAGA ACATGGTTGG AGAATGGGCC
    TTACCAGAAG AAATGGTTGA AAACTTACCA CCCTCCAACA ATTGCATATT GGGCCATGTG
    ATTCACAGAC TAATTGAAAG TTCTCTTCCT CCTCAAGTTG AATCTACAGT ACCTGAGTTA
    CCTTCATTTG CTATAAAAGG ATGCTTGTTG GGGAAAACCT TTAGTGGAAA AACTACTGCT
    CTAAAGGCTC TAGAGAAAAG CTTTCCTATT CAGGTACTTT CTATTGACAC TCTTGTTCAA
    GAAGCTATCC AAGCATTTCA TGACAATGAA AGTATCAGCG GGGCCCATGG ACTTCAGAAA
    GCACCTGAAG AAAAGGTTTT ACCAGTTAGG CGAAAGAGTG GTAAAGAAAA CCAGGACGTG
    CAAGATGAAG CACTACCAGA AAGAGAAGAT AAAACCAGAC TTGATACCGA TGCTAGAAAA
    GCAGCAAAAG CTGAAGAAGT CGTAGCGAGT GATAGTTTCT CTGAACTCAC TGTGCGTGCT
    CAGCTTGGTG CAAAATCAGA AATGCTGCTG AAGAAAGGAA AGAGCATATC TGATATATTG
    CTAGTCAGCA TCATGGTAAA TGCCATTAAT CAGATACCTA TGTATAAAGG CTGGATCCTG
    GATGGCTTTC CAATGACATT AAACCAAGCA AAACTTCTGG AGGAAGCACT CACAGGCTAC
    AACAGAAACC TCATAGAACT GGAGGGAAAG AAATCACAAA CATCCACATT AGCTGTTGAC
    CCTACAGCTT CCAAAGAAGT GCCTCTTCCC CCTTCTGCAT TTGATTTTGT CATGTTATTA
    GACATTTCAG ATAATTCCTC ACTGAATCGC ATGAATGACA TCATGGCTAA AGCATTTTCA
    AGTGAAGCTC CTCGTGAAGA TATCAATCAG CGTGTAGCTG CTAAAAACAA AGATACAGAT
    GAGGACCAGA ATTTAAGAGA TCAAATACAG CACAGAATTG TAGGCTTCCT GGACAACTGG
    CCTTCGTTGG AGCAGTGGTT TACAGAGCCA GAAAACATTT TGATAAAAAT CAATGCTGAA
    GTAGATAAAC AGTCATTGTT CCAAAAAGTA AAGGAAATGA TTATGACTGA AATAATTAAA
    AAAGAAAATA AAGTGAAAAA GAAATTAGAA GAAAAGGAAG CTGAGAGAAA AGAAGAAGTT
    TCCCTGAGTG AGAAGACATC CCCTCCTCCT CCTCCCCCTC CGGAACCAGA GAAAGAGAAG
    GAAGTCCACC TTCCACAGGA GCGGTCCAAA ACTCCTGCTG CAAAAGGAAA GCAAGCATCA
    GAACCCCTGC ATGGTAATCG AGAACCTCTT CAGGAAGGAA AAGGGAAGAA AAGTGAAACT
    TCACTCAAAA GAAAAGGTTC TCCTAGAGGA AAATCGCTAG GAGGGAAGAT TCCACTCAAG
    AAATCACCTG CCGAGTCTAC AGATATTTCA CCGGTCCCGG TAGTGCCAGC CCCATCGAAG
    CCCGGATCAG AAGAGTGGGT GTATGTAAAT GAACCAATTC CTGAGGAAAT ACCATCATTT
    TTAGTACCAT ACTGGAAATT GATAGAAAAT TCCTATATAA ATTCCATCAA AACAGTACTC
    AGGGAGTTGA GAGAAGGCCA GCATAGTGTA CTTGCTTACT TATATGATAT TAGAACAGGC
    TTCCAGCAGT TTCTGAAGCG TCCAGACCAC AAGCAAGACT TCGTGTCTCA GTGGCAGGCG
    GATTTCAACT CCCTTCCTGA TGACCTGTGG GATGATGAGG AAACAAAGGC TGAGCTGCAC
    CAAAGAGTGA ATGATTTACG GGACCGCCTG TGGGACATTT GCGATGCCCG TAAGGAAGAA
    GCAGAGCAGG AGCGTCTGGA CATCATTAAC GAGAGCTGGT TGCAGGACTC AATGGGAATT
    GCCATGAACC ATTTCTTCTC ACTGATGCAG GCAGAACTGA ACCGTTTCCA AGATACGAAG
    AGATTCCTGC AAGATTATTA CAGGGCCATG GAATGTAAAA TTCCGATGGA TGACACTAAG
    AAATTCACTC GAGTCCCACT GGTCCGACTG GATAGTAGAG ATATTTTGGA AAGCCAGCTT
    AGAATTCCCC TGGTTCCTCG AAGATCCATT TCTCCAGAGT CAGCTTTATC CAAACCAAAA
    ACAAAAAGCA TCCTGAAGGG CAAGATCGAT TACACGCTGG AAAGTGTAGA GTTAAACTTT
    GAGACTGATG AGAAGATGGT GATGGATACG TGGCAGCAGT CTTCTTTGGC AGTATCTCAC
    ATGGTGGCTG CTGAAATTCA TCAGAGGCTC ATGGAAGAAG AAAAAGAAAA CCAGCCAGCA
    GAAACAAAAG AAAAACTTCC TCAGGCAGCT GCAAATAAAA AAGTGAAAAA GGAGCCTCCG
    CCACCGCCAC CACCACCCAA GAAAAAGAAA GAGGACAAAA AAGGAAAAGG TAAATCCCCA
    CCTATGGCAG AAGCTACTCC GGTAGTAGTG CCAGCAGAAG AGACTGAGGA AGTGGAATTA
    AAAAATGAAC TGAAGCTCAG AATAAAGGAA GAGCATCTTG CTGCCCTGCA ATTTGAAGAG
    ATAGCTACAC AGTTCCGACT TGAACTGATA AAGGTAAAAG CATTGGCTTT CCTGGAAGAG
    TTAGTAATAA AGGCGGTTGA TGTATATAGA CTCATGGACA AGTGGCTCGG TGAGAGGTAC
    TTGAATGAAA TGGCCAGTGT GGAAAAACTA ACTGGGGTAG CTCGCTATCA CATTGAAACA
    TCAACCAAAA TTCAGAATGA ACTTTATTTA GACCAAGAAG ACTTCTTCAT TAATGGCGAT
    ATCAAAGTCT TTCCAGATCC ACCTCCTCCG GTACGCCCTC CGCCTGTGGA AAAGGAGGAA
    AATGGCACCC TGACCATGGA GCAGCTTGAC AACCTCCGGG ATCAGTTCTT AGATATAGCG
    CCCAAAGGCA GGGATCCTCC AGACAATGTG AGTGGATTCA CCAAGAAGCA GAAAAGGCCT
    CGGAAGCTTG ACCATTACCT CCCAAAGAGG AACAATCAAT TTTTGAAAAT TTGGCTTAAA
    AAATACCCTT GGCTTGTATA CGATGAGATA CTAAATCTTA TGTTCTGCGG CCTGTGTCGT
    AAACATGGAG TGACATTAAA GGGAAATCAG GTCAGTTTTT TTTATGGCAC GGATAACTTC
    AGGACTGAAT TTCTCAACGC GCATCATCTG AGTGAGGCTC ACGCCAAGGC GTCCCTCATG
    GAAGCCACGA GTGGTTCTCC AGGGAGCAGA GCTACCACGG AGCTGATGGT GAGGACCATG
    AGCAAGGTGA CCCTTGGCAG GGTGGAGAAC CTGTTCCGGT CCTGCCACGC CATCGCCAAG
    ACCGGACGTC CCCTCAAAGA CTTCCTCTGG ATGTGTTCGC TGGACGATAT GAAGGGAGTT
    GATATCGGCC CGGTGTTCAG AACCAGTAAA TCGGCCAGAA CTTTTACCTA CTTTATTGCT
    GAAGTGGAAC GGAGAACTCT AAGAGAGAAG TTAGAAAAAA GTCAGTTCTT CTCCGTCCTC
    AGTGATGGGA TTGCAGACAG TTCAGCTGAG GAAGCTGAAC TCGTATATGT GCAGTTTGCC
    CGCGCAGGGA GAGTGCACTG CCAAATCGTC GGGGTACAGA CCGTGGAGAA GAAGGAGCCC
    TGGGCAATAA AACGTGCCAT CGAGAAAACT CTGGAGAGAA ATCTTCAACT TCGACTGTCG
    AACCAAGACT GGGCAAAGAA ACTGGTGGGT TTCGGAAGCG ATGGAGCTCC AGGTTCAGAG
    GGAGAAAACG GCAGCACAGT GGCCCTGCTT TTAAGGGAAA TCCAGCCAGG TGTGCTGCCT
    GTTTACTGCC TTGCCCATCA CCTCGAACTG TCTTACCAGG CGGTGTTCCA GAGCGTTCCC
    CTCTACAGCC GTGTGAAAGA CCTTCTTCAT AGCATGCACC GCTTCTATCA CGATTCTCCT
    GCTCACAGGA GTGCTCTGGT GACTGCTTTC AAGGGCCTCC ACCTTCAACC TGCGATGCCC
    TCTCAAGTCA GAGGCCGGAG GTGGCTTCAG GGATTGCAGG CTGCCCTCCA GAACTTTCTC
    AAGGGATATC CAGCAATTGT CCAGCAGCTG CAGTCAACAG AAGAAAGCAG AAGCAACAGC
    AGTCATCAGA AAGCCGAAGA ACTCCTGGAG TCTCTCCTCC AGGCAGACAT TGTCAAATTT
    GTCCACTTCT TGGTGGATGT CGTCAATGTC CTTAGCATAC TGTCCCATGT CAATCAGAAC
    AGAAATTCCT CAATTGCTGA TGTATTTGCC ACCTTAGAGT CAACACTGGA AATGCTCCAA
    ATTTATCAGA CAAGACCAGG GCCGAAAGAA CGTGGGGTAG ATTCGGTCAC ACACTTTCAT
    GGTAACTGCC TTGGAGGAAA GGGAAACATT TCTGACGTGA GAAACATTGT TTTAACACAT
    CTTATCAAGA GGCTGCGAGG CTGTTTCAGA GATGCCAACC AAGATGTGGT GAAGGCAACC
    ACGGTTGGAA GCTTTAAACT GTGGCCGGCA AAAATAAATC AAGAATTTGG AGAAAAAGAG
    GTGGCCATCC TGATTGCACA TTATGAACCA GTATTAGAGG CAGCCAAGGT GAAAATTGAC
    CAAGTAGACA CAGAATGGAG CATGTTGAAA TTGGAGATCT ACTCTAGATT TCAGAACATT
    TGCAAACTGA CCTGGGATTT TGTGAATTCT GTTTATTTAC ACAAGTATCC AAACATCCTG
    ACACTCATTG ACCTTGTGCT GAGCCTGCCT GCAAGTTCAG CTGAGGCAGG ACGTGGCTTT
    AGTCAGATGA AGCGCATCAG GTCCCAAATG CATGCCAAGG TCAAGGCTGA CAACATGACG
    GATCTCCTGA TAATTCAGTT GAATTCTCCA GACATTAACA ACTTTGACCC TAGGAAAGCC
    ATTCATTTAT GGAATACAAG AACACCATCC TCAACTGAGG ACACAAGACC TAACTCAGAC
    TGCTCATCAA ACTCAGAAAG CCTTAGTGAA AGCGATAAGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OOv13492
    Clone ID Related Accession (Same CDS sequence) XM_015106495.1
    Accession Version XM_015106495.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5133bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-16
    Organism Ovis aries musimon(Ovis orientalis musimon)
    Product sperm flagellar protein 2 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942355.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGCATGATT GGAAGGAACT GTTTTTCCAT GGAAAACCCA TATATGAGCA AGCCTCTCTT 
    AGACATTTAC CAGCTAAGCT CTCTGCAGAA CACCTTGTAG AAATGGAGAA AAGGAACTTG
    CTAGATAACA ATGATTATGA AGAATATAAG AACATGGTTG GAGAATGGGC CTTACCAGAA
    GAAATGGTTG AAAACTTACC ACCCTCCAAC AATTGCATAT TGGGCCATGT GATTCACAGA
    CTAATTGAAA GTTCTCTTCC TCCTCAAGTT GAATCTACAG TACCTGAGTT ACCTTCATTT
    GCTATAAAAG GATGCTTGTT GGGGAAAACC TTTAGTGGAA AAACTACTGC TCTAAAGGCT
    CTAGAGAAAA GCTTTCCTAT TCAGGTACTT TCTATTGACA CTCTTGTTCA AGAAGCTATC
    CAAGCATTTC ATGACAATGA AAGTATCAGC GGGGCCCATG GACTTCAGAA AGCACCTGAA
    GAAAAGGTTT TACCAGTTAG GCGAAAGAGT GGTAAAGAAA ACCAGGACGT GCAAGATGAA
    GCACTACCAG AAAGAGAAGA TAAAACCAGA CTTGATACCG ATGCTAGAAA AGCAGCAAAA
    GCTGAAGAAG TCGTAGCGAG TGATAGTTTC TCTGAACTCA CTGTGCGTGC TCAGCTTGGT
    GCAAAATCAG AAATGCTGCT GAAGAAAGGA AAGAGCATAT CTGATATATT GCTAGTCAGC
    ATCATGGTAA ATGCCATTAA TCAGATACCT ATGTATAAAG GCTGGATCCT GGATGGCTTT
    CCAATGACAT TAAACCAAGC AAAACTTCTG GAGGAAGCAC TCACAGGCTA CAACAGAAAC
    CTCATAGAAC TGGAGGGAAA GAAATCACAA ACATCCACAT TAGCTGTTGA CCCTACAGCT
    TCCAAAGAAG TGCCTCTTCC CCCTTCTGCA TTTGATTTTG TCATGTTATT AGACATTTCA
    GATAATTCCT CACTGAATCG CATGAATGAC ATCATGGCTA AAGCATTTTC AAGTGAAGCT
    CCTCGTGAAG ATATCAATCA GCGTGTAGCT GCTAAAAACA AAGATACAGA TGAGGACCAG
    AATTTAAGAG ATCAAATACA GCACAGAATT GTAGGCTTCC TGGACAACTG GCCTTCGTTG
    GAGCAGTGGT TTACAGAGCC AGAAAACATT TTGATAAAAA TCAATGCTGA AGTAGATAAA
    CAGTCATTGT TCCAAAAAGT AAAGGAAATG ATTATGACTG AAATAATTAA AAAAGAAAAT
    AAAGTGAAAA AGAAATTAGA AGAAAAGGAA GCTGAGAGAA AAGAAGAAGT TTCCCTGAGT
    GAGAAGACAT CCCCTCCTCC TCCTCCCCCT CCGGAACCAG AGAAAGAGAA GGAAGTCCAC
    CTTCCACAGG AGCGGTCCAA AACTCCTGCT GCAAAAGGAA AGCAAGCATC AGAACCCCTG
    CATGGTAATC GAGAACCTCT TCAGGAAGGA AAAGGGAAGA AAAGTGAAAC TTCACTCAAA
    AGAAAAGGTT CTCCTAGAGG AAAATCGCTA GGAGGGAAGA TTCCACTCAA GAAATCACCT
    GCCGAGTCTA CAGATATTTC ACCGGTCCCG GTAGTGCCAG CCCCATCGAA GCCCGGATCA
    GAAGAGTGGG TGTATGTAAA TGAACCAATT CCTGAGGAAA TACCATCATT TTTAGTACCA
    TACTGGAAAT TGATAGAAAA TTCCTATATA AATTCCATCA AAACAGTACT CAGGGAGTTG
    AGAGAAGGCC AGCATAGTGT ACTTGCTTAC TTATATGATA TTAGAACAGG CTTCCAGCAG
    TTTCTGAAGC GTCCAGACCA CAAGCAAGAC TTCGTGTCTC AGTGGCAGGC GGATTTCAAC
    TCCCTTCCTG ATGACCTGTG GGATGATGAG GAAACAAAGG CTGAGCTGCA CCAAAGAGTG
    AATGATTTAC GGGACCGCCT GTGGGACATT TGCGATGCCC GTAAGGAAGA AGCAGAGCAG
    GAGCGTCTGG ACATCATTAA CGAGAGCTGG TTGCAGGACT CAATGGGAAT TGCCATGAAC
    CATTTCTTCT CACTGATGCA GGCAGAACTG AACCGTTTCC AAGATACGAA GAGATTCCTG
    CAAGATTATT ACAGGGCCAT GGAATGTAAA ATTCCGATGG ATGACACTAA GAAATTCACT
    CGAGTCCCAC TGGTCCGACT GGATAGTAGA GATATTTTGG AAAGCCAGCT TAGAATTCCC
    CTGGTTCCTC GAAGATCCAT TTCTCCAGAG TCAGCTTTAT CCAAACCAAA AACAAAAAGC
    ATCCTGAAGG GCAAGATCGA TTACACGCTG GAAAGTGTAG AGTTAAACTT TGAGACTGAT
    GAGAAGATGG TGATGGATAC GTGGCAGCAG TCTTCTTTGG CAGTATCTCA CATGGTGGCT
    GCTGAAATTC ATCAGAGGCT CATGGAAGAA GAAAAAGAAA ACCAGCCAGC AGAAACAAAA
    GAAAAACTTC CTCAGGCAGC TGCAAATAAA AAAGTGAAAA AGGAGCCTCC GCCACCGCCA
    CCACCACCCA AGAAAAAGAA AGAGGACAAA AAAGGAAAAG GTAAATCCCC ACCTATGGCA
    GAAGCTACTC CGGTAGTAGT GCCAGCAGAA GAGACTGAGG AAGTGGAATT AAAAAATGAA
    CTGAAGCTCA GAATAAAGGA AGAGCATCTT GCTGCCCTGC AATTTGAAGA GATAGCTACA
    CAGTTCCGAC TTGAACTGAT AAAGGTAAAA GCATTGGCTT TCCTGGAAGA GTTAGTAATA
    AAGGCGGTTG ATGTATATAG ACTCATGGAC AAGTGGCTCG GTGAGAGGTA CTTGAATGAA
    ATGGCCAGTG TGGAAAAACT AACTGGGGTA GCTCGCTATC ACATTGAAAC ATCAACCAAA
    ATTCAGAATG AACTTTATTT AGACCAAGAA GACTTCTTCA TTAATGGCGA TATCAAAGTC
    TTTCCAGATC CACCTCCTCC GGTACGCCCT CCGCCTGTGG AAAAGGAGGA AAATGGCACC
    CTGACCATGG AGCAGCTTGA CAACCTCCGG GATCAGTTCT TAGATATAGC GCCCAAAGGC
    AGGGATCCTC CAGACAATGT GAGTGGATTC ACCAAGAAGC AGAAAAGGCC TCGGAAGCTT
    GACCATTACC TCCCAAAGAG GAACAATCAA TTTTTGAAAA TTTGGCTTAA AAAATACCCT
    TGGCTTGTAT ACGATGAGAT ACTAAATCTT ATGTTCTGCG GCCTGTGTCG TAAACATGGA
    GTGACATTAA AGGGAAATCA GGTCAGTTTT TTTTATGGCA CGGATAACTT CAGGACTGAA
    TTTCTCAACG CGCATCATCT GAGTGAGGCT CACGCCAAGG CGTCCCTCAT GGAAGCCACG
    AGTGGTTCTC CAGGGAGCAG AGCTACCACG GAGCTGATGG TGAGGACCAT GAGCAAGGTG
    ACCCTTGGCA GGGTGGAGAA CCTGTTCCGG TCCTGCCACG CCATCGCCAA GACCGGACGT
    CCCCTCAAAG ACTTCCTCTG GATGTGTTCG CTGGACGATA TGAAGGGAGT TGATATCGGC
    CCGGTGTTCA GAACCAGTAA ATCGGCCAGA ACTTTTACCT ACTTTATTGC TGAAGTGGAA
    CGGAGAACTC TAAGAGAGAA GTTAGAAAAA AGTCAGTTCT TCTCCGTCCT CAGTGATGGG
    ATTGCAGACA GTTCAGCTGA GGAAGCTGAA CTCGTATATG TGCAGTTTGC CCGCGCAGGG
    AGAGTGCACT GCCAAATCGT CGGGGTACAG ACCGTGGAGA AGAAGGAGCC CTGGGCAATA
    AAACGTGCCA TCGAGAAAAC TCTGGAGAGA AATCTTCAAC TTCGACTGTC GAACCAAGAC
    TGGGCAAAGA AACTGGTGGG TTTCGGAAGC GATGGAGCTC CAGGTTCAGA GGGAGAAAAC
    GGCAGCACAG TGGCCCTGCT TTTAAGGGAA ATCCAGCCAG GTGTGCTGCC TGTTTACTGC
    CTTGCCCATC ACCTCGAACT GTCTTACCAG GCGGTGTTCC AGAGCGTTCC CCTCTACAGC
    CGTGTGAAAG ACCTTCTTCA TAGCATGCAC CGCTTCTATC ACGATTCTCC TGCTCACAGG
    AGTGCTCTGG TGACTGCTTT CAAGGGCCTC CACCTTCAAC CTGCGATGCC CTCTCAAGTC
    AGAGGCCGGA GGTGGCTTCA GGGATTGCAG GCTGCCCTCC AGAACTTTCT CAAGGGATAT
    CCAGCAATTG TCCAGCAGCT GCAGTCAACA GAAGAAAGCA GAAGCAACAG CAGTCATCAG
    AAAGCCGAAG AACTCCTGGA GTCTCTCCTC CAGGCAGACA TTGTCAAATT TGTCCACTTC
    TTGGTGGATG TCGTCAATGT CCTTAGCATA CTGTCCCATG TCAATCAGAA CAGAAATTCC
    TCAATTGCTG ATGTATTTGC CACCTTAGAG TCAACACTGG AAATGCTCCA AATTTATCAG
    ACAAGACCAG GGCCGAAAGA ACGTGGGGTA GATTCGGTCA CACACTTTCA TGGTAACTGC
    CTTGGAGGAA AGGGAAACAT TTCTGACGTG AGAAACATTG TTTTAACACA TCTTATCAAG
    AGGCTGCGAG GCTGTTTCAG AGATGCCAAC CAAGATGTGG TGAAGGCAAC CACGGTTGGA
    AGCTTTAAAC TGTGGCCGGC AAAAATAAAT CAAGAATTTG GAGAAAAAGA GGTGGCCATC
    CTGATTGCAC ATTATGAACC AGTATTAGAG GCAGCCAAGG TGAAAATTGA CCAAGTAGAC
    ACAGAATGGA GCATGTTGAA ATTGGAGATC TACTCTAGAT TTCAGAACAT TTGCAAACTG
    ACCTGGGATT TTGTGAATTC TGTTTATTTA CACAAGTATC CAAACATCCT GACACTCATT
    GACCTTGTGC TGAGCCTGCC TGCAAGTTCA GCTGAGGCAG GACGTGGCTT TAGTCAGATG
    AAGCGCATCA GGTCCCAAAT GCATGCCAAG GTCAAGGCTG ACAACATGAC GGATCTCCTG
    ATAATTCAGT TGAATTCTCC AGACATTAAC AACTTTGACC CTAGGAAAGC CATTCATTTA
    TGGAATACAA GAACACCATC CTCAACTGAG GACACAAGAC CTAACTCAGA CTGCTCATCA
    AACTCAGAAA GCCTTAGTGA AAGCGATAAG TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_014961981.1
    CDS1339..6471
    Translation

    Target ORF information:

    RefSeq Version XM_015106495.1
    Organism Ovis aries musimon(Ovis orientalis musimon)
    Definition Ovis aries musimon sperm flagellar 2 (SPEF2), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015106495.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGCATGATT GGAAGGAACT GTTTTTCCAT GGAAAACCCA TATATGAGCA AGCCTCTCTT 
    AGACATTTAC CAGCTAAGCT CTCTGCAGAA CACCTTGTAG AAATGGAGAA AAGGAACTTG
    CTAGATAACA ATGATTATGA AGAATATAAG AACATGGTTG GAGAATGGGC CTTACCAGAA
    GAAATGGTTG AAAACTTACC ACCCTCCAAC AATTGCATAT TGGGCCATGT GATTCACAGA
    CTAATTGAAA GTTCTCTTCC TCCTCAAGTT GAATCTACAG TACCTGAGTT ACCTTCATTT
    GCTATAAAAG GATGCTTGTT GGGGAAAACC TTTAGTGGAA AAACTACTGC TCTAAAGGCT
    CTAGAGAAAA GCTTTCCTAT TCAGGTACTT TCTATTGACA CTCTTGTTCA AGAAGCTATC
    CAAGCATTTC ATGACAATGA AAGTATCAGC GGGGCCCATG GACTTCAGAA AGCACCTGAA
    GAAAAGGTTT TACCAGTTAG GCGAAAGAGT GGTAAAGAAA ACCAGGACGT GCAAGATGAA
    GCACTACCAG AAAGAGAAGA TAAAACCAGA CTTGATACCG ATGCTAGAAA AGCAGCAAAA
    GCTGAAGAAG TCGTAGCGAG TGATAGTTTC TCTGAACTCA CTGTGCGTGC TCAGCTTGGT
    GCAAAATCAG AAATGCTGCT GAAGAAAGGA AAGAGCATAT CTGATATATT GCTAGTCAGC
    ATCATGGTAA ATGCCATTAA TCAGATACCT ATGTATAAAG GCTGGATCCT GGATGGCTTT
    CCAATGACAT TAAACCAAGC AAAACTTCTG GAGGAAGCAC TCACAGGCTA CAACAGAAAC
    CTCATAGAAC TGGAGGGAAA GAAATCACAA ACATCCACAT TAGCTGTTGA CCCTACAGCT
    TCCAAAGAAG TGCCTCTTCC CCCTTCTGCA TTTGATTTTG TCATGTTATT AGACATTTCA
    GATAATTCCT CACTGAATCG CATGAATGAC ATCATGGCTA AAGCATTTTC AAGTGAAGCT
    CCTCGTGAAG ATATCAATCA GCGTGTAGCT GCTAAAAACA AAGATACAGA TGAGGACCAG
    AATTTAAGAG ATCAAATACA GCACAGAATT GTAGGCTTCC TGGACAACTG GCCTTCGTTG
    GAGCAGTGGT TTACAGAGCC AGAAAACATT TTGATAAAAA TCAATGCTGA AGTAGATAAA
    CAGTCATTGT TCCAAAAAGT AAAGGAAATG ATTATGACTG AAATAATTAA AAAAGAAAAT
    AAAGTGAAAA AGAAATTAGA AGAAAAGGAA GCTGAGAGAA AAGAAGAAGT TTCCCTGAGT
    GAGAAGACAT CCCCTCCTCC TCCTCCCCCT CCGGAACCAG AGAAAGAGAA GGAAGTCCAC
    CTTCCACAGG AGCGGTCCAA AACTCCTGCT GCAAAAGGAA AGCAAGCATC AGAACCCCTG
    CATGGTAATC GAGAACCTCT TCAGGAAGGA AAAGGGAAGA AAAGTGAAAC TTCACTCAAA
    AGAAAAGGTT CTCCTAGAGG AAAATCGCTA GGAGGGAAGA TTCCACTCAA GAAATCACCT
    GCCGAGTCTA CAGATATTTC ACCGGTCCCG GTAGTGCCAG CCCCATCGAA GCCCGGATCA
    GAAGAGTGGG TGTATGTAAA TGAACCAATT CCTGAGGAAA TACCATCATT TTTAGTACCA
    TACTGGAAAT TGATAGAAAA TTCCTATATA AATTCCATCA AAACAGTACT CAGGGAGTTG
    AGAGAAGGCC AGCATAGTGT ACTTGCTTAC TTATATGATA TTAGAACAGG CTTCCAGCAG
    TTTCTGAAGC GTCCAGACCA CAAGCAAGAC TTCGTGTCTC AGTGGCAGGC GGATTTCAAC
    TCCCTTCCTG ATGACCTGTG GGATGATGAG GAAACAAAGG CTGAGCTGCA CCAAAGAGTG
    AATGATTTAC GGGACCGCCT GTGGGACATT TGCGATGCCC GTAAGGAAGA AGCAGAGCAG
    GAGCGTCTGG ACATCATTAA CGAGAGCTGG TTGCAGGACT CAATGGGAAT TGCCATGAAC
    CATTTCTTCT CACTGATGCA GGCAGAACTG AACCGTTTCC AAGATACGAA GAGATTCCTG
    CAAGATTATT ACAGGGCCAT GGAATGTAAA ATTCCGATGG ATGACACTAA GAAATTCACT
    CGAGTCCCAC TGGTCCGACT GGATAGTAGA GATATTTTGG AAAGCCAGCT TAGAATTCCC
    CTGGTTCCTC GAAGATCCAT TTCTCCAGAG TCAGCTTTAT CCAAACCAAA AACAAAAAGC
    ATCCTGAAGG GCAAGATCGA TTACACGCTG GAAAGTGTAG AGTTAAACTT TGAGACTGAT
    GAGAAGATGG TGATGGATAC GTGGCAGCAG TCTTCTTTGG CAGTATCTCA CATGGTGGCT
    GCTGAAATTC ATCAGAGGCT CATGGAAGAA GAAAAAGAAA ACCAGCCAGC AGAAACAAAA
    GAAAAACTTC CTCAGGCAGC TGCAAATAAA AAAGTGAAAA AGGAGCCTCC GCCACCGCCA
    CCACCACCCA AGAAAAAGAA AGAGGACAAA AAAGGAAAAG GTAAATCCCC ACCTATGGCA
    GAAGCTACTC CGGTAGTAGT GCCAGCAGAA GAGACTGAGG AAGTGGAATT AAAAAATGAA
    CTGAAGCTCA GAATAAAGGA AGAGCATCTT GCTGCCCTGC AATTTGAAGA GATAGCTACA
    CAGTTCCGAC TTGAACTGAT AAAGGTAAAA GCATTGGCTT TCCTGGAAGA GTTAGTAATA
    AAGGCGGTTG ATGTATATAG ACTCATGGAC AAGTGGCTCG GTGAGAGGTA CTTGAATGAA
    ATGGCCAGTG TGGAAAAACT AACTGGGGTA GCTCGCTATC ACATTGAAAC ATCAACCAAA
    ATTCAGAATG AACTTTATTT AGACCAAGAA GACTTCTTCA TTAATGGCGA TATCAAAGTC
    TTTCCAGATC CACCTCCTCC GGTACGCCCT CCGCCTGTGG AAAAGGAGGA AAATGGCACC
    CTGACCATGG AGCAGCTTGA CAACCTCCGG GATCAGTTCT TAGATATAGC GCCCAAAGGC
    AGGGATCCTC CAGACAATGT GAGTGGATTC ACCAAGAAGC AGAAAAGGCC TCGGAAGCTT
    GACCATTACC TCCCAAAGAG GAACAATCAA TTTTTGAAAA TTTGGCTTAA AAAATACCCT
    TGGCTTGTAT ACGATGAGAT ACTAAATCTT ATGTTCTGCG GCCTGTGTCG TAAACATGGA
    GTGACATTAA AGGGAAATCA GGTCAGTTTT TTTTATGGCA CGGATAACTT CAGGACTGAA
    TTTCTCAACG CGCATCATCT GAGTGAGGCT CACGCCAAGG CGTCCCTCAT GGAAGCCACG
    AGTGGTTCTC CAGGGAGCAG AGCTACCACG GAGCTGATGG TGAGGACCAT GAGCAAGGTG
    ACCCTTGGCA GGGTGGAGAA CCTGTTCCGG TCCTGCCACG CCATCGCCAA GACCGGACGT
    CCCCTCAAAG ACTTCCTCTG GATGTGTTCG CTGGACGATA TGAAGGGAGT TGATATCGGC
    CCGGTGTTCA GAACCAGTAA ATCGGCCAGA ACTTTTACCT ACTTTATTGC TGAAGTGGAA
    CGGAGAACTC TAAGAGAGAA GTTAGAAAAA AGTCAGTTCT TCTCCGTCCT CAGTGATGGG
    ATTGCAGACA GTTCAGCTGA GGAAGCTGAA CTCGTATATG TGCAGTTTGC CCGCGCAGGG
    AGAGTGCACT GCCAAATCGT CGGGGTACAG ACCGTGGAGA AGAAGGAGCC CTGGGCAATA
    AAACGTGCCA TCGAGAAAAC TCTGGAGAGA AATCTTCAAC TTCGACTGTC GAACCAAGAC
    TGGGCAAAGA AACTGGTGGG TTTCGGAAGC GATGGAGCTC CAGGTTCAGA GGGAGAAAAC
    GGCAGCACAG TGGCCCTGCT TTTAAGGGAA ATCCAGCCAG GTGTGCTGCC TGTTTACTGC
    CTTGCCCATC ACCTCGAACT GTCTTACCAG GCGGTGTTCC AGAGCGTTCC CCTCTACAGC
    CGTGTGAAAG ACCTTCTTCA TAGCATGCAC CGCTTCTATC ACGATTCTCC TGCTCACAGG
    AGTGCTCTGG TGACTGCTTT CAAGGGCCTC CACCTTCAAC CTGCGATGCC CTCTCAAGTC
    AGAGGCCGGA GGTGGCTTCA GGGATTGCAG GCTGCCCTCC AGAACTTTCT CAAGGGATAT
    CCAGCAATTG TCCAGCAGCT GCAGTCAACA GAAGAAAGCA GAAGCAACAG CAGTCATCAG
    AAAGCCGAAG AACTCCTGGA GTCTCTCCTC CAGGCAGACA TTGTCAAATT TGTCCACTTC
    TTGGTGGATG TCGTCAATGT CCTTAGCATA CTGTCCCATG TCAATCAGAA CAGAAATTCC
    TCAATTGCTG ATGTATTTGC CACCTTAGAG TCAACACTGG AAATGCTCCA AATTTATCAG
    ACAAGACCAG GGCCGAAAGA ACGTGGGGTA GATTCGGTCA CACACTTTCA TGGTAACTGC
    CTTGGAGGAA AGGGAAACAT TTCTGACGTG AGAAACATTG TTTTAACACA TCTTATCAAG
    AGGCTGCGAG GCTGTTTCAG AGATGCCAAC CAAGATGTGG TGAAGGCAAC CACGGTTGGA
    AGCTTTAAAC TGTGGCCGGC AAAAATAAAT CAAGAATTTG GAGAAAAAGA GGTGGCCATC
    CTGATTGCAC ATTATGAACC AGTATTAGAG GCAGCCAAGG TGAAAATTGA CCAAGTAGAC
    ACAGAATGGA GCATGTTGAA ATTGGAGATC TACTCTAGAT TTCAGAACAT TTGCAAACTG
    ACCTGGGATT TTGTGAATTC TGTTTATTTA CACAAGTATC CAAACATCCT GACACTCATT
    GACCTTGTGC TGAGCCTGCC TGCAAGTTCA GCTGAGGCAG GACGTGGCTT TAGTCAGATG
    AAGCGCATCA GGTCCCAAAT GCATGCCAAG GTCAAGGCTG ACAACATGAC GGATCTCCTG
    ATAATTCAGT TGAATTCTCC AGACATTAAC AACTTTGACC CTAGGAAAGC CATTCATTTA
    TGGAATACAA GAACACCATC CTCAACTGAG GACACAAGAC CTAACTCAGA CTGCTCATCA
    AACTCAGAAA GCCTTAGTGA AAGCGATAAG TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OSm66603
    Clone ID Related Accession (Same CDS sequence) XM_012097083.3
    Accession Version XM_012097083.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5277bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-05
    Organism Ovis aries(sheep)
    Product sperm flagellar protein 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040267.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Feb 6, 2019 this sequence version replaced XM_012097083.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ovis aries Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGTCGGAAA TCCTGTGCCA GTGGCTCAAC CAGGAATTGA AGGTGTCCCA GACCGTGAGT 
    CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAA TTGGAGAAGT TTTGCACAAG
    TTTGCACTTC AGGATGATTT TGCAGACTTT TCAGAAAGCA GGATTTCCAG TGCCAGACTT
    AACAATTTTT CTCGCTTGGA GCCCACACTT CACCTTTTGG GTGTGGAATT TGATCAGAAT
    GTGGCCCACA ATATCATCAC AGAGAAGCCT GGGGCTGCCA CGAAACTCTT ATATCAGTTG
    TACATTGCTC TCCAGAGGAA AAAGAAAGGT GTTCAGACTA GAGTGGAAAT GCCAACTGTA
    CAGCCCCTGA TGAGCACAAA AGTTCAAAAC ATGAAAAGCG AGGCTTTCCG AGATCAACGC
    TTGAGCAAAA GACGACAAAA TGAAATCATG GCCAAAATCC AAGCAGCTAT CATACAGATT
    CCTAAACCCA CATCAAATCG TACTCTGAAA ACATTTGACG CCCAAAAATT AATGAAAAAG
    AAAAAAGAGG CAGAGGATGT GGCCAATGAA ATTAAGAAGT TTGAAGCATT AATAAAAAAG
    GATCTCCAGG CAAAAGAAAG TGCATCCAAG ACTTCTCTAG ATACAGCAGG CCAGACAACC
    GCTGATTTGT TAAACACTTA TTCAGATGAT GACTACATTA AAAAAATCCA AAAACGCCTT
    GAAGAAGATG CTTTTGCACG AGAGCAAAGG GAGAAAAGAC GGCGGAGACT CTTAATGGAT
    CAGTTAATAG CTCATGAAGC CCAAGAGGAG GCTTATCGGG AGGAGCAGTT GATCAACCGG
    CTGATGCGAC AGTCCCAGCA GGAACGCAGG ATCGCTGTGC AGCTCATGCA CGTTAGGCAT
    GAAAAGGAAG TTTTATGGCA AAACAGAATT TTCAGAGAAA AACAATATGA GGAAAGACGG
    CTTAAAGACT TCCAGGATGC TCTTGATCGA GAAGCGGCTT TAGCCAGACA AGCCAAGATT
    GATTTTGAAG AACAAGCCCT CAGAGATAAG GAAATTCATG AGCAGATCGC CATAGAAAGA
    GCTCAAGCTC GTTACAAAAA GCATTATTCA ATATGTGCAG AAATTTTGGA TCAGATACTT
    GATCTGTCCA CTAAGGTGGC AGACTATCGA TTGTTGACAA ATAACCTGAT CCCATATAAG
    TTGATGCATG ATTGGAAGGA ACTGTTTTTC CATGGAAAAC CCATATATGA GCAAGCCTCT
    CTTAGACATT TACCAGCTAA GCTCTCTGCA GAACACCTTG TAGAAATGGA GAAAAGGAAC
    TTGCTAGATA ACAATGATTA TGAAGAATAT AAGAACATGG TTGGAGAATG GGCCTTACCA
    GAAGAAATGG TTGAAAACTT ACCACCCTCC AACAATTGCA TATTGGGCCA TGTGATTCAC
    AGACTAATTG AAAGTTCTCT TCCTCCTCAA GTTGAATCTA CAGTACCTGA GTTACCTTCA
    TTTGCTATAA AAGGATGCTT GTTGGGGAAA ACCTTTAGTG GAAAAACTAC TGCTCTAAAG
    GCTCTAGAGA AAAGCTTTCC TATTCAGGTA CTTTCTATTG ACACTCTTGT TCAAGAAGCT
    ATCCAAGCAT TTCATGACAA TGAAAGTATC AGCGGGGCCC ATGGACTTCA GAAAGCACCT
    GAAGAAAAGG TTTTACCAGT TAGGCGAAAG AGTGGTAAAG AAAACCAGGA CGTGCAAGAT
    GAAGCACTAC CAGAAAGAGA AGATAAAACC AGACTTGATA CCGATGCTAG AAAAGCAGCA
    AAAGCTGAAG AAGTCGTAGC GAGTGATAGT TTCTCTGAAC TCACTGTGCG TGCTCAGCTT
    GGTGCAAAAT CAGAAATGCT GCTGAAGAAA GGAAAGAGCA TATCTGATAT ATTGCTAGTC
    AGCATCATGG TAAATGCCAT TAATCAGATA CCTATGTATA AAGGCTGGAT CCTGGATGGC
    TTTCCAATGA CATTAAACCA AGCAAAACTT CTGGAGGAAG CACTCACAGG CTACAACAGA
    AACCTCATAG AACTGGAGGG AAAGAAATCA CAAACATCCA CATTAGCTGT TGACCCTACA
    GCTTCCAAAG AAGTGCCTCT TCCCCCTTCT GCATTTGATT TTGTCATGTT ATTAGACATT
    TCAGATAATT CCTCACTGAA TCGCATGAAT GACATCATGG CTAAAGCATT TTCAAGTGAA
    GCTCCTCGTG AAGATACCAA TCAGCGTGTA GCTGCTAAAA ACAAAGATAC AGATGAGGAC
    CAGAATTTAA GAGATCAAAT ACAGCACAGA ATTGTAGGCT TCCTGGACAA CTGGCCTTCG
    TTGGAGCAGT GGTTTACAGA GCCAGAAAAC ATTTTGATAA AAATCAATGC TGAAGTAGAT
    AAACAGTCAT TGTTCCAAAA AGTAAAGGAA ATGATTATGA CTGAAATAAT TAAAAAAGAA
    AATAAAGTGA AAAAGAAATT AGAAGAAAAG GAAGCTGAGA GAAAAGAAGA AGTTTCCCTG
    AGTGAGAAGA CATCCCCTCC TCCTCCTCCC CCTCCGGAAC CAGAGAAAGA GAAGGAAGTC
    CACCTTCCAC AGGAGCGGTC CAAAACTCCT GCTGCAAAAG GAAAGCAAGC ATCAGAACCC
    CTGCATGGTA ATCGAGAACC TCTTCAGGAA GGAAAAGGGA AGAAAAGTGA AACTTCACTC
    AAAAGAAAAG GTTCTCCTAG AGGAAAATCG CTAGGAGGGA AGATTCCACT CAAGAAATCA
    CCTGCCGAGT CTACAGATAT TTCACCGGTC CCGGTAGTGC CAGCCCCATC GAAGCCCGGA
    TCAGAAGAGT GGGTGTATGT AAATGAACCA ATTCCTGAGG AAATACCATC ATTTTTAGTA
    CCATACTGGA AATTGATAGA AAACTCCTAT ATAAATTCCA TCAAAACAGT ACTCAGGGAG
    TTGAGAGAAG GCCAGCATAG TGTACTTGCT TACTTATATG ATATTAGAAC AGGCTTCCAG
    CAGTTTCTGA AGCGTCCAGA CCACAAGCAA GACTTCGTGT CTCAGTGGCA GGCGGATTTC
    AACTCCCTTC CTGATGACCT GTGGGATGAT GAGGAAACAA AGGCTGAGCT GCACCAAAGA
    GTGAATGATT TACGGGACCG CCTGTGGGAC ATTTGCGATG CCCGTAAGGA AGAAGCAGAG
    CAGGAGCGTC TGGACATCAT TAACGAGAGC TGGTTGCAGG ACTCAATGGG AATTGCCATG
    AACCATTTCT TCTCACTGAT GCAGGCAGAA CTGAACCGTT TCCAAGATAC GAAGAGATTC
    CTGCAAGATT ATTACAGGGC CATGGAATGT AAAATTCCGA TGGATGACAC TAAGAAATTC
    ACTCGAGTCC CACTGGTCCG ACTGGATAGT AGAGATATTT TGGAAAGCCA GCTTAGAATT
    CCCCTGGTTC CTCGAAGATC CATTTCTCCA GAGTCAGCTT TATCCAAACC AAAAACAAAA
    AGCATCCTGA AGGGCAAGAT CGATTACACG CTGGAAAGTG TAGAGTTAAA CTTTGAGACT
    GATGAGAAGA TGGTGATGGA TACGTGGCAG CAGTCTTCTT TGGCAGTATC TCACATGGTG
    GCTGCTGAAA TTCATCAGAG GCTCATGGAA GAAGAAAAAG AAAACCAGCC AGCAGAAACA
    AAAGAAAAAC TTCCTCAGGC AGCTGCAAAT AAAAAAGTGA AAAAGGAGCC TCCGCCACCG
    CCACCACCAC CCAAGAAAAA GAAAGAGGAC AAAAAAGGAA AAGGTAAATC CCCACCTATG
    GCAGAAGCTA CTCCTGTAGT AGTGCCAGCA GAAGAGACTG AGGAAGTGGA ATTAAAAAAT
    GAACTGAAGC TCAGAATAAA GGAAGAGCAT CTTGCTGCCC TGCAATTTGA AGAGATAGCT
    ACACAGTTCC GACTTGAACT GATAAAGGTA AAAGCATTGG CTTTCCTGGA AGAGTTAGTA
    ATAAAGGCGG TTGATGTATA TAGACTCATG GACAAGTGGC TCGGTGAGAG GTACTTGAAT
    GAAATGGCCA GTGTGGAAAA ACTAACTGGG GTAGCTCGCT ATCACATTGA AACATCAACC
    AAAATTCAGA ATGAACTTTA TTTAGACCAA GAAGACTTCT TCATTAATGG CGATATCAAA
    GTCTTTCCAG ATCCACCTCC TCCGGTACGC CCTCCGCCTG TGGAAAAGGA GGAAAATGGC
    ACCCTGACCA TGGAGCAGCT TGACAACCTC CGGGATCAGT TCTTAGATAT AGCGCCCAAA
    GGTATAATAG GAAATAAAGC ATTTGCTGAC ATTCTGCTGG ATTTGGTAAC TCTGAACCTT
    GGAACAAACA ACTTTCCTAC TAGTTGGATG AACCTCACTC AACCGGAATT GCAGGAGTTA
    GCATCTTTGT TGGTAACAAA CTCGGAGTCC GTGGACTGGC GGAAATTCTT GTTAGTAGTA
    GCGCTGCCTT GGCCCATCCC CCTGGAGGAG GAGCTTCTGG AGACCCTGCA GAGGTTCAAG
    GCCCTGGATG CGGAGCAGCT GGGCACCGTC ACTTATGAAC AGTATAAGCA GGCTGGCCTG
    TGGTTTACAG GAGATGAAGA TATCAAAATT CCAGAAAATC CTCTTGAACC CCTGCCATTC
    AATAGGCAGG AGCATCTTAT AGAGTTTTTC TTTAGGTTGT TTGCTGACTG CGAGAAGGTT
    CCACCCCAGC TTGACTACAC ACAGATGCTG CTTTATTTTG CTTGCCATCC AGACAGCACA
    GAAGGGGTCT ACAGGGCCCT CAGCGTAGCC ATTGGGACTC ACGTCTTCCA GCCAATTGAA
    ATGCCTCATC CTGTTGCAGC AAAGACCTCT TCCTTTACTG ATATGAGTCC CATTGAGGAA
    TATCCTGAAC TCGAGGACAA CTTTATGAGT GAGGAAAGGG AATTACAAGA GGACGGGGAG
    GAAAAGGAAG AAGAAATCCC TGAAAATGCA AATACGGAAA TGATTTCCAT GCAAACATTA
    CTCCAAGTGT TCCGAGGAGG AAATGAAGTA CTGGACGCTA ACAGATTTGC CAGCCACCAA
    AAGATAGAGA ACATTTATTT AGAGAACTTC ATAAAAGTGT TTCAGGACCT AGGTTCCAAG
    AACCTTGAGC CAATTGAAGT TGCTGTTCTT CTGAAGCATC CTTTTATTCA AGACCTGATT
    TCAAGTTATC CAGACTATAA GATCCCTGAT GTTAAGATAA TTCTCCAAAG GAGTGAGCAT
    GTACAAGGAA GTGATGAAGA GAGGTCTCCT TCAAGACTTA CAGAGGAAAA GAAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011952473.2
    CDS77..5353
    Translation

    Target ORF information:

    RefSeq Version XM_012097083.3
    Organism Ovis aries(sheep)
    Definition Ovis aries sperm flagellar protein 2 (LOC101111394), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012097083.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGTCGGAAA TCCTGTGCCA GTGGCTCAAC CAGGAATTGA AGGTGTCCCA GACCGTGAGT 
    CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAA TTGGAGAAGT TTTGCACAAG
    TTTGCACTTC AGGATGATTT TGCAGACTTT TCAGAAAGCA GGATTTCCAG TGCCAGACTT
    AACAATTTTT CTCGCTTGGA GCCCACACTT CACCTTTTGG GTGTGGAATT TGATCAGAAT
    GTGGCCCACA ATATCATCAC AGAGAAGCCT GGGGCTGCCA CGAAACTCTT ATATCAGTTG
    TACATTGCTC TCCAGAGGAA AAAGAAAGGT GTTCAGACTA GAGTGGAAAT GCCAACTGTA
    CAGCCCCTGA TGAGCACAAA AGTTCAAAAC ATGAAAAGCG AGGCTTTCCG AGATCAACGC
    TTGAGCAAAA GACGACAAAA TGAAATCATG GCCAAAATCC AAGCAGCTAT CATACAGATT
    CCTAAACCCA CATCAAATCG TACTCTGAAA ACATTTGACG CCCAAAAATT AATGAAAAAG
    AAAAAAGAGG CAGAGGATGT GGCCAATGAA ATTAAGAAGT TTGAAGCATT AATAAAAAAG
    GATCTCCAGG CAAAAGAAAG TGCATCCAAG ACTTCTCTAG ATACAGCAGG CCAGACAACC
    GCTGATTTGT TAAACACTTA TTCAGATGAT GACTACATTA AAAAAATCCA AAAACGCCTT
    GAAGAAGATG CTTTTGCACG AGAGCAAAGG GAGAAAAGAC GGCGGAGACT CTTAATGGAT
    CAGTTAATAG CTCATGAAGC CCAAGAGGAG GCTTATCGGG AGGAGCAGTT GATCAACCGG
    CTGATGCGAC AGTCCCAGCA GGAACGCAGG ATCGCTGTGC AGCTCATGCA CGTTAGGCAT
    GAAAAGGAAG TTTTATGGCA AAACAGAATT TTCAGAGAAA AACAATATGA GGAAAGACGG
    CTTAAAGACT TCCAGGATGC TCTTGATCGA GAAGCGGCTT TAGCCAGACA AGCCAAGATT
    GATTTTGAAG AACAAGCCCT CAGAGATAAG GAAATTCATG AGCAGATCGC CATAGAAAGA
    GCTCAAGCTC GTTACAAAAA GCATTATTCA ATATGTGCAG AAATTTTGGA TCAGATACTT
    GATCTGTCCA CTAAGGTGGC AGACTATCGA TTGTTGACAA ATAACCTGAT CCCATATAAG
    TTGATGCATG ATTGGAAGGA ACTGTTTTTC CATGGAAAAC CCATATATGA GCAAGCCTCT
    CTTAGACATT TACCAGCTAA GCTCTCTGCA GAACACCTTG TAGAAATGGA GAAAAGGAAC
    TTGCTAGATA ACAATGATTA TGAAGAATAT AAGAACATGG TTGGAGAATG GGCCTTACCA
    GAAGAAATGG TTGAAAACTT ACCACCCTCC AACAATTGCA TATTGGGCCA TGTGATTCAC
    AGACTAATTG AAAGTTCTCT TCCTCCTCAA GTTGAATCTA CAGTACCTGA GTTACCTTCA
    TTTGCTATAA AAGGATGCTT GTTGGGGAAA ACCTTTAGTG GAAAAACTAC TGCTCTAAAG
    GCTCTAGAGA AAAGCTTTCC TATTCAGGTA CTTTCTATTG ACACTCTTGT TCAAGAAGCT
    ATCCAAGCAT TTCATGACAA TGAAAGTATC AGCGGGGCCC ATGGACTTCA GAAAGCACCT
    GAAGAAAAGG TTTTACCAGT TAGGCGAAAG AGTGGTAAAG AAAACCAGGA CGTGCAAGAT
    GAAGCACTAC CAGAAAGAGA AGATAAAACC AGACTTGATA CCGATGCTAG AAAAGCAGCA
    AAAGCTGAAG AAGTCGTAGC GAGTGATAGT TTCTCTGAAC TCACTGTGCG TGCTCAGCTT
    GGTGCAAAAT CAGAAATGCT GCTGAAGAAA GGAAAGAGCA TATCTGATAT ATTGCTAGTC
    AGCATCATGG TAAATGCCAT TAATCAGATA CCTATGTATA AAGGCTGGAT CCTGGATGGC
    TTTCCAATGA CATTAAACCA AGCAAAACTT CTGGAGGAAG CACTCACAGG CTACAACAGA
    AACCTCATAG AACTGGAGGG AAAGAAATCA CAAACATCCA CATTAGCTGT TGACCCTACA
    GCTTCCAAAG AAGTGCCTCT TCCCCCTTCT GCATTTGATT TTGTCATGTT ATTAGACATT
    TCAGATAATT CCTCACTGAA TCGCATGAAT GACATCATGG CTAAAGCATT TTCAAGTGAA
    GCTCCTCGTG AAGATACCAA TCAGCGTGTA GCTGCTAAAA ACAAAGATAC AGATGAGGAC
    CAGAATTTAA GAGATCAAAT ACAGCACAGA ATTGTAGGCT TCCTGGACAA CTGGCCTTCG
    TTGGAGCAGT GGTTTACAGA GCCAGAAAAC ATTTTGATAA AAATCAATGC TGAAGTAGAT
    AAACAGTCAT TGTTCCAAAA AGTAAAGGAA ATGATTATGA CTGAAATAAT TAAAAAAGAA
    AATAAAGTGA AAAAGAAATT AGAAGAAAAG GAAGCTGAGA GAAAAGAAGA AGTTTCCCTG
    AGTGAGAAGA CATCCCCTCC TCCTCCTCCC CCTCCGGAAC CAGAGAAAGA GAAGGAAGTC
    CACCTTCCAC AGGAGCGGTC CAAAACTCCT GCTGCAAAAG GAAAGCAAGC ATCAGAACCC
    CTGCATGGTA ATCGAGAACC TCTTCAGGAA GGAAAAGGGA AGAAAAGTGA AACTTCACTC
    AAAAGAAAAG GTTCTCCTAG AGGAAAATCG CTAGGAGGGA AGATTCCACT CAAGAAATCA
    CCTGCCGAGT CTACAGATAT TTCACCGGTC CCGGTAGTGC CAGCCCCATC GAAGCCCGGA
    TCAGAAGAGT GGGTGTATGT AAATGAACCA ATTCCTGAGG AAATACCATC ATTTTTAGTA
    CCATACTGGA AATTGATAGA AAACTCCTAT ATAAATTCCA TCAAAACAGT ACTCAGGGAG
    TTGAGAGAAG GCCAGCATAG TGTACTTGCT TACTTATATG ATATTAGAAC AGGCTTCCAG
    CAGTTTCTGA AGCGTCCAGA CCACAAGCAA GACTTCGTGT CTCAGTGGCA GGCGGATTTC
    AACTCCCTTC CTGATGACCT GTGGGATGAT GAGGAAACAA AGGCTGAGCT GCACCAAAGA
    GTGAATGATT TACGGGACCG CCTGTGGGAC ATTTGCGATG CCCGTAAGGA AGAAGCAGAG
    CAGGAGCGTC TGGACATCAT TAACGAGAGC TGGTTGCAGG ACTCAATGGG AATTGCCATG
    AACCATTTCT TCTCACTGAT GCAGGCAGAA CTGAACCGTT TCCAAGATAC GAAGAGATTC
    CTGCAAGATT ATTACAGGGC CATGGAATGT AAAATTCCGA TGGATGACAC TAAGAAATTC
    ACTCGAGTCC CACTGGTCCG ACTGGATAGT AGAGATATTT TGGAAAGCCA GCTTAGAATT
    CCCCTGGTTC CTCGAAGATC CATTTCTCCA GAGTCAGCTT TATCCAAACC AAAAACAAAA
    AGCATCCTGA AGGGCAAGAT CGATTACACG CTGGAAAGTG TAGAGTTAAA CTTTGAGACT
    GATGAGAAGA TGGTGATGGA TACGTGGCAG CAGTCTTCTT TGGCAGTATC TCACATGGTG
    GCTGCTGAAA TTCATCAGAG GCTCATGGAA GAAGAAAAAG AAAACCAGCC AGCAGAAACA
    AAAGAAAAAC TTCCTCAGGC AGCTGCAAAT AAAAAAGTGA AAAAGGAGCC TCCGCCACCG
    CCACCACCAC CCAAGAAAAA GAAAGAGGAC AAAAAAGGAA AAGGTAAATC CCCACCTATG
    GCAGAAGCTA CTCCTGTAGT AGTGCCAGCA GAAGAGACTG AGGAAGTGGA ATTAAAAAAT
    GAACTGAAGC TCAGAATAAA GGAAGAGCAT CTTGCTGCCC TGCAATTTGA AGAGATAGCT
    ACACAGTTCC GACTTGAACT GATAAAGGTA AAAGCATTGG CTTTCCTGGA AGAGTTAGTA
    ATAAAGGCGG TTGATGTATA TAGACTCATG GACAAGTGGC TCGGTGAGAG GTACTTGAAT
    GAAATGGCCA GTGTGGAAAA ACTAACTGGG GTAGCTCGCT ATCACATTGA AACATCAACC
    AAAATTCAGA ATGAACTTTA TTTAGACCAA GAAGACTTCT TCATTAATGG CGATATCAAA
    GTCTTTCCAG ATCCACCTCC TCCGGTACGC CCTCCGCCTG TGGAAAAGGA GGAAAATGGC
    ACCCTGACCA TGGAGCAGCT TGACAACCTC CGGGATCAGT TCTTAGATAT AGCGCCCAAA
    GGTATAATAG GAAATAAAGC ATTTGCTGAC ATTCTGCTGG ATTTGGTAAC TCTGAACCTT
    GGAACAAACA ACTTTCCTAC TAGTTGGATG AACCTCACTC AACCGGAATT GCAGGAGTTA
    GCATCTTTGT TGGTAACAAA CTCGGAGTCC GTGGACTGGC GGAAATTCTT GTTAGTAGTA
    GCGCTGCCTT GGCCCATCCC CCTGGAGGAG GAGCTTCTGG AGACCCTGCA GAGGTTCAAG
    GCCCTGGATG CGGAGCAGCT GGGCACCGTC ACTTATGAAC AGTATAAGCA GGCTGGCCTG
    TGGTTTACAG GAGATGAAGA TATCAAAATT CCAGAAAATC CTCTTGAACC CCTGCCATTC
    AATAGGCAGG AGCATCTTAT AGAGTTTTTC TTTAGGTTGT TTGCTGACTG CGAGAAGGTT
    CCACCCCAGC TTGACTACAC ACAGATGCTG CTTTATTTTG CTTGCCATCC AGACAGCACA
    GAAGGGGTCT ACAGGGCCCT CAGCGTAGCC ATTGGGACTC ACGTCTTCCA GCCAATTGAA
    ATGCCTCATC CTGTTGCAGC AAAGACCTCT TCCTTTACTG ATATGAGTCC CATTGAGGAA
    TATCCTGAAC TCGAGGACAA CTTTATGAGT GAGGAAAGGG AATTACAAGA GGACGGGGAG
    GAAAAGGAAG AAGAAATCCC TGAAAATGCA AATACGGAAA TGATTTCCAT GCAAACATTA
    CTCCAAGTGT TCCGAGGAGG AAATGAAGTA CTGGACGCTA ACAGATTTGC CAGCCACCAA
    AAGATAGAGA ACATTTATTT AGAGAACTTC ATAAAAGTGT TTCAGGACCT AGGTTCCAAG
    AACCTTGAGC CAATTGAAGT TGCTGTTCTT CTGAAGCATC CTTTTATTCA AGACCTGATT
    TCAAGTTATC CAGACTATAA GATCCCTGAT GTTAAGATAA TTCTCCAAAG GAGTGAGCAT
    GTACAAGGAA GTGATGAAGA GAGGTCTCCT TCAAGACTTA CAGAGGAAAA GAAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OSm66604
    Clone ID Related Accession (Same CDS sequence) XM_027980125.1
    Accession Version XM_027980125.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5094bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-05
    Organism Ovis aries(sheep)
    Product sperm flagellar protein 2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040267.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ovis aries Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGTCGGAAA TCCTGTGCCA GTGGCTCAAC CAGGAATTGA AGGTGTCCCA GACCGTGAGT 
    CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAA TTGGAGAAGT TTTGCACAAG
    TTTGCACTTC AGGATGATTT TGCAGACTTT TCAGAAAGCA GGATTTCCAG TGCCAGACTT
    AACAATTTTT CTCGCTTGGA GCCCACACTT CACCTTTTGG GTGTGGAATT TGATCAGAAT
    GTGGCCCACA ATATCATCAC AGAGAAGCCT GGGGCTGCCA CGAAACTCTT ATATCAGTTG
    TACATTGCTC TCCAGAGGAA AAAGAAAGGT GTTCAGACTA GAGTGGAAAT GCCAACTGTA
    CAGCCCCTGA TGAGCACAAA AGTTCAAAAC ATGAAAAGCG AGGCTTTCCG AGATCAACGC
    TTGAGCAAAA GACGACAAAA TGAAATCATG GCCAAAATCC AAGCAGCTAT CATACAGATT
    CCTAAACCCA CATCAAATCG TACTCTGAAA ACATTTGACG CCCAAAAATT AATGAAAAAG
    AAAAAAGAGG CAGAGGATGT GGCCAATGAA ATTAAGAAGT TTGAAGCATT AATAAAAAAG
    GATCTCCAGG CAAAAGAAAG TGCATCCAAG ACTTCTCTAG ATACAGCAGG CCAGACAACC
    GCTGATTTGT TAAACACTTA TTCAGATGAT GACTACATTA AAAAAATCCA AAAACGCCTT
    GAAGAAGATG CTTTTGCACG AGAGCAAAGG GAGAAAAGAC GGCGGAGACT CTTAATGGAT
    CAGTTAATAG CTCATGAAGC CCAAGAGGAG GCTTATCGGG AGGAGCAGTT GATCAACCGG
    CTGATGCGAC AGTCCCAGCA GGAACGCAGG ATCGCTGTGC AGCTCATGCA CGTTAGGCAT
    GAAAAGGAAG TTTTATGGCA AAACAGAATT TTCAGAGAAA AACAATATGA GGAAAGACGG
    CTTAAAGACT TCCAGGATGC TCTTGATCGA GAAGCGGCTT TAGCCAGACA AGCCAAGATT
    GATTTTGAAG AACAAGCCCT CAGAGATAAG GAAATTCATG AGCAGATCGC CATAGAAAGA
    GCTCAAGCTC GTTACAAAAA GCATTATTCA ATATGTGCAG AAATTTTGGA TCAGATACTT
    GATCTGTCCA CTAAGGTGGC AGACTATCGA TTGTTGACAA ATAACCTGAT CCCATATAAG
    TTGATGCATG ATTGGAAGGA ACTGTTTTTC CATGGAAAAC CCATATATGA GCAAGCCTCT
    CTTAGACATT TACCAGCTAA GCTCTCTGCA GAACACCTTG TAGAAATGGA GAAAAGGAAC
    TTGCTAGATA ACAATGATTA TGAAGAATAT AAGAACATGG TTGGAGAATG GGCCTTACCA
    GAAGAAATGG TTGAAAACTT ACCACCCTCC AACAATTGCA TATTGGGCCA TGTGATTCAC
    AGACTAATTG AAAGTTCTCT TCCTCCTCAA GTTGAATCTA CAGTACCTGA GTTACCTTCA
    TTTGCTATAA AAGGATGCTT GTTGGGGAAA ACCTTTAGTG GAAAAACTAC TGCTCTAAAG
    GCTCTAGAGA AAAGCTTTCC TATTCAGGTA CTTTCTATTG ACACTCTTGT TCAAGAAGCT
    ATCCAAGCAT TTCATGACAA TGAAAGTATC AGCGGGGCCC ATGGACTTCA GAAAGCACCT
    GAAGAAAAGG TTTTACCAGT TAGGCGAAAG AGTGGTAAAG AAAACCAGGA CGTGCAAGAT
    GAAGCACTAC CAGAAAGAGA AGATAAAACC AGACTTGATA CCGATGCTAG AAAAGCAGCA
    AAAGCTGAAG AAGTCGTAGC GAGTGATAGT TTCTCTGAAC TCACTGTGCG TGCTCAGCTT
    GGTGCAAAAT CAGAAATGCT GCTGAAGAAA GGAAAGAGCA TATCTGATAT ATTGCTAGTC
    AGCATCATGG TAAATGCCAT TAATCAGATA CCTATGTATA AAGGCTGGAT CCTGGATGGC
    TTTCCAATGA CATTAAACCA AGCAAAACTT CTGGAGGAAG CACTCACAGG CTACAACAGA
    AACCTCATAG AACTGGAGGG AAAGAAATCA CAAACATCCA CATTAGCTGT TGACCCTACA
    GCTTCCAAAG AAGTGCCTCT TCCCCCTTCT GCATTTGATT TTGTCATGTT ATTAGACATT
    TCAGATAATT CCTCACTGAA TCGCATGAAT GACATCATGG CTAAAGCATT TTCAAGTGAA
    GCTCCTCGTG AAGATACCAA TCAGCGTGTA GCTGCTAAAA ACAAAGATAC AGATGAGGAC
    CAGAATTTAA GAGATCAAAT ACAGCACAGA ATTGTAGGCT TCCTGGACAA CTGGCCTTCG
    TTGGAGCAGT GGTTTACAGA GCCAGAAAAC ATTTTGATAA AAATCAATGC TGAAGTAGAT
    AAACAGTCAT TGTTCCAAAA AGTAAAGGAA ATGATTATGA CTGAAATAAT TAAAAAAGAA
    AATAAAGTGA AAAAGAAATT AGAAGAAAAG GAAGCTGAGA GAAAAGAAGA AGTTTCCCTG
    AGTGAGAAGA CATCCCCTCC TCCTCCTCCC CCTCCGGAAC CAGAGAAAGA GAAGGAAGTC
    CACCTTCCAC AGGAGCGGTC CAAAACTCCT GCTGCAAAAG GAAAGCAAGC ATCAGAACCC
    CTGCATGGTA ATCGAGAACC TCTTCAGGAA GGAAAAGGGA AGAAAAGTGA AACTTCACTC
    AAAAGAAAAG GTTCTCCTAG AGGAAAATCG CTAGGAGGGA AGATTCCACT CAAGAAATCA
    CCTGCCGAGT CTACAGATAT TTCACCGGTC CCGGTAGTGC CAGCCCCATC GAAGCCCGGA
    TCAGAAGAGT GGGTGTATGT AAATGAACCA ATTCCTGAGG AAATACCATC ATTTTTAGTA
    CCATACTGGA AATTGATAGA AAACTCCTAT ATAAATTCCA TCAAAACAGT ACTCAGGGAG
    TTGAGAGAAG GCCAGCATAG TGTACTTGCT TACTTATATG ATATTAGAAC AGGCTTCCAG
    CAGTTTCTGA AGCGTCCAGA CCACAAGCAA GACTTCGTGT CTCAGTGGCA GGCGGATTTC
    AACTCCCTTC CTGATGACCT GTGGGATGAT GAGGAAACAA AGGCTGAGCT GCACCAAAGA
    GTGAATGATT TACGGGACCG CCTGTGGGAC ATTTGCGATG CCCGTAAGGA AGAAGCAGAG
    CAGGAGCGTC TGGACATCAT TAACGAGAGC TGGTTGCAGG ACTCAATGGG AATTGCCATG
    AACCATTTCT TCTCACTGAT GCAGGCAGAA CTGAACCGTT TCCAAGATAC GAAGAGATTC
    CTGCAAGATT ATTACAGGGC CATGGAATGT AAAATTCCGA TGGATGACAC TAAGAAATTC
    ACTCGAGTCC CACTGGTCCG ACTGGATAGT AGAGATATTT TGGAAAGCCA GCTTAGAATT
    CCCCTGGTTC CTCGAAGATC CATTTCTCCA GAGTCAGCTT TATCCAAACC AAAAACAAAA
    AGCATCCTGA AGGGCAAGAT CGATTACACG CTGGAAAGTG TAGAGTTAAA CTTTGAGACT
    GATGAGAAGA TGGTGATGGA TACGTGGCAG CAGTCTTCTT TGGCAGTATC TCACATGGTG
    GCTGCTGAAA TTCATCAGAG GCTCATGGAA GAAGAAAAAG AAAACCAGCC AGCAGAAACA
    AAAGAAAAAC TTCCTCAGGC AGCTGCAAAT AAAAAAGTGA AAAAGGAGCC TCCGCCACCG
    CCACCACCAC CCAAGAAAAA GAAAGAGGAC AAAAAAGGAA AAGGTAAATC CCCACCTATG
    GCAGAAGCTA CTCCTGTAGT AGTGCCAGCA GAAGAGACTG AGGAAGTGGA ATTAAAAAAT
    GAACTGAAGC TCAGAATAAA GGAAGAGCAT CTTGCTGCCC TGCAATTTGA AGAGATAGCT
    ACACAGTTCC GACTTGAACT GATAAAGGTA AAAGCATTGG CTTTCCTGGA AGAGTTAGTA
    ATAAAGGCGG TTGATGTATA TAGACTCATG GACAAGTGGC TCGGTGAGAG GTACTTGAAT
    GAAATGGCCA GTGTGGAAAA ACTAACTGGG GTAGCTCGCT ATCACATTGA AACATCAACC
    AAAATTCAGA ATGAACTTTA TTTAGACCAA GAAGACTTCT TCATTAATGG CGATATCAAA
    GTCTTTCCAG ATCCACCTCC TCCGGTACGC CCTCCGCCTG TGGAAAAGGA GGAAAATGGC
    ACCCTGACCA TGGAGCAGCT TGACAACCTC CGGGATCAGT TCTTAGATAT AGCGCCCAAA
    GGTATAATAG GAAATAAAGC ATTTGCTGAC ATTCTGCTGG ATTTGGTAAC TCTGAACCTT
    GGAACAAACA ACTTTCCTAC TAGTTGGATG AACCTCACTC AACCGGAAGC TGGCCTGTGG
    TTTACAGGAG ATGAAGATAT CAAAATTCCA GAAAATCCTC TTGAACCCCT GCCATTCAAT
    AGGCAGGAGC ATCTTATAGA GTTTTTCTTT AGGTTGTTTG CTGACTGCGA GAAGGTTCCA
    CCCCAGCTTG ACTACACACA GATGCTGCTT TATTTTGCTT GCCATCCAGA CAGCACAGAA
    GGGGTCTACA GGGCCCTCAG CGTAGCCATT GGGACTCACG TCTTCCAGCC AATTGAAATG
    CCTCATCCTG TTGCAGCAAA GACCTCTTCC TTTACTGATA TGAGTCCCAT TGAGGAATAT
    CCTGAACTCG AGGACAACTT TATGAGTGAG GAAAGGGAAT TACAAGAGGA CGGGGAGGAA
    AAGGAAGAAG AAATCCCTGA AAATGCAAAT ACGGAAATGA TTTCCATGCA AACATTACTC
    CAAGTGTTCC GAGGAGGAAA TGAAGTACTG GACGCTAACA GATTTGCCAG CCACCAAAAG
    ATAGAGAACA TTTATTTAGA GAACTTCATA AAAGTGTTTC AGGACCTAGG TTCCAAGAAC
    CTTGAGCCAA TTGAAGTTGC TGTTCTTCTG AAGCATCCTT TTATTCAAGA CCTGATTTCA
    AGTTATCCAG ACTATAAGAT CCCTGATGTT AAGATAATTC TCCAAAGGAG TGAGCATGTA
    CAAGGAAGTG ATGAAGAGAG GTCTCCTTCA AGACTTACAG AGGAAAAGAA ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_027835926.1
    CDS77..5170
    Translation

    Target ORF information:

    RefSeq Version XM_027980125.1
    Organism Ovis aries(sheep)
    Definition Ovis aries sperm flagellar protein 2 (LOC101111394), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_027980125.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGTCGGAAA TCCTGTGCCA GTGGCTCAAC CAGGAATTGA AGGTGTCCCA GACCGTGAGT 
    CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAA TTGGAGAAGT TTTGCACAAG
    TTTGCACTTC AGGATGATTT TGCAGACTTT TCAGAAAGCA GGATTTCCAG TGCCAGACTT
    AACAATTTTT CTCGCTTGGA GCCCACACTT CACCTTTTGG GTGTGGAATT TGATCAGAAT
    GTGGCCCACA ATATCATCAC AGAGAAGCCT GGGGCTGCCA CGAAACTCTT ATATCAGTTG
    TACATTGCTC TCCAGAGGAA AAAGAAAGGT GTTCAGACTA GAGTGGAAAT GCCAACTGTA
    CAGCCCCTGA TGAGCACAAA AGTTCAAAAC ATGAAAAGCG AGGCTTTCCG AGATCAACGC
    TTGAGCAAAA GACGACAAAA TGAAATCATG GCCAAAATCC AAGCAGCTAT CATACAGATT
    CCTAAACCCA CATCAAATCG TACTCTGAAA ACATTTGACG CCCAAAAATT AATGAAAAAG
    AAAAAAGAGG CAGAGGATGT GGCCAATGAA ATTAAGAAGT TTGAAGCATT AATAAAAAAG
    GATCTCCAGG CAAAAGAAAG TGCATCCAAG ACTTCTCTAG ATACAGCAGG CCAGACAACC
    GCTGATTTGT TAAACACTTA TTCAGATGAT GACTACATTA AAAAAATCCA AAAACGCCTT
    GAAGAAGATG CTTTTGCACG AGAGCAAAGG GAGAAAAGAC GGCGGAGACT CTTAATGGAT
    CAGTTAATAG CTCATGAAGC CCAAGAGGAG GCTTATCGGG AGGAGCAGTT GATCAACCGG
    CTGATGCGAC AGTCCCAGCA GGAACGCAGG ATCGCTGTGC AGCTCATGCA CGTTAGGCAT
    GAAAAGGAAG TTTTATGGCA AAACAGAATT TTCAGAGAAA AACAATATGA GGAAAGACGG
    CTTAAAGACT TCCAGGATGC TCTTGATCGA GAAGCGGCTT TAGCCAGACA AGCCAAGATT
    GATTTTGAAG AACAAGCCCT CAGAGATAAG GAAATTCATG AGCAGATCGC CATAGAAAGA
    GCTCAAGCTC GTTACAAAAA GCATTATTCA ATATGTGCAG AAATTTTGGA TCAGATACTT
    GATCTGTCCA CTAAGGTGGC AGACTATCGA TTGTTGACAA ATAACCTGAT CCCATATAAG
    TTGATGCATG ATTGGAAGGA ACTGTTTTTC CATGGAAAAC CCATATATGA GCAAGCCTCT
    CTTAGACATT TACCAGCTAA GCTCTCTGCA GAACACCTTG TAGAAATGGA GAAAAGGAAC
    TTGCTAGATA ACAATGATTA TGAAGAATAT AAGAACATGG TTGGAGAATG GGCCTTACCA
    GAAGAAATGG TTGAAAACTT ACCACCCTCC AACAATTGCA TATTGGGCCA TGTGATTCAC
    AGACTAATTG AAAGTTCTCT TCCTCCTCAA GTTGAATCTA CAGTACCTGA GTTACCTTCA
    TTTGCTATAA AAGGATGCTT GTTGGGGAAA ACCTTTAGTG GAAAAACTAC TGCTCTAAAG
    GCTCTAGAGA AAAGCTTTCC TATTCAGGTA CTTTCTATTG ACACTCTTGT TCAAGAAGCT
    ATCCAAGCAT TTCATGACAA TGAAAGTATC AGCGGGGCCC ATGGACTTCA GAAAGCACCT
    GAAGAAAAGG TTTTACCAGT TAGGCGAAAG AGTGGTAAAG AAAACCAGGA CGTGCAAGAT
    GAAGCACTAC CAGAAAGAGA AGATAAAACC AGACTTGATA CCGATGCTAG AAAAGCAGCA
    AAAGCTGAAG AAGTCGTAGC GAGTGATAGT TTCTCTGAAC TCACTGTGCG TGCTCAGCTT
    GGTGCAAAAT CAGAAATGCT GCTGAAGAAA GGAAAGAGCA TATCTGATAT ATTGCTAGTC
    AGCATCATGG TAAATGCCAT TAATCAGATA CCTATGTATA AAGGCTGGAT CCTGGATGGC
    TTTCCAATGA CATTAAACCA AGCAAAACTT CTGGAGGAAG CACTCACAGG CTACAACAGA
    AACCTCATAG AACTGGAGGG AAAGAAATCA CAAACATCCA CATTAGCTGT TGACCCTACA
    GCTTCCAAAG AAGTGCCTCT TCCCCCTTCT GCATTTGATT TTGTCATGTT ATTAGACATT
    TCAGATAATT CCTCACTGAA TCGCATGAAT GACATCATGG CTAAAGCATT TTCAAGTGAA
    GCTCCTCGTG AAGATACCAA TCAGCGTGTA GCTGCTAAAA ACAAAGATAC AGATGAGGAC
    CAGAATTTAA GAGATCAAAT ACAGCACAGA ATTGTAGGCT TCCTGGACAA CTGGCCTTCG
    TTGGAGCAGT GGTTTACAGA GCCAGAAAAC ATTTTGATAA AAATCAATGC TGAAGTAGAT
    AAACAGTCAT TGTTCCAAAA AGTAAAGGAA ATGATTATGA CTGAAATAAT TAAAAAAGAA
    AATAAAGTGA AAAAGAAATT AGAAGAAAAG GAAGCTGAGA GAAAAGAAGA AGTTTCCCTG
    AGTGAGAAGA CATCCCCTCC TCCTCCTCCC CCTCCGGAAC CAGAGAAAGA GAAGGAAGTC
    CACCTTCCAC AGGAGCGGTC CAAAACTCCT GCTGCAAAAG GAAAGCAAGC ATCAGAACCC
    CTGCATGGTA ATCGAGAACC TCTTCAGGAA GGAAAAGGGA AGAAAAGTGA AACTTCACTC
    AAAAGAAAAG GTTCTCCTAG AGGAAAATCG CTAGGAGGGA AGATTCCACT CAAGAAATCA
    CCTGCCGAGT CTACAGATAT TTCACCGGTC CCGGTAGTGC CAGCCCCATC GAAGCCCGGA
    TCAGAAGAGT GGGTGTATGT AAATGAACCA ATTCCTGAGG AAATACCATC ATTTTTAGTA
    CCATACTGGA AATTGATAGA AAACTCCTAT ATAAATTCCA TCAAAACAGT ACTCAGGGAG
    TTGAGAGAAG GCCAGCATAG TGTACTTGCT TACTTATATG ATATTAGAAC AGGCTTCCAG
    CAGTTTCTGA AGCGTCCAGA CCACAAGCAA GACTTCGTGT CTCAGTGGCA GGCGGATTTC
    AACTCCCTTC CTGATGACCT GTGGGATGAT GAGGAAACAA AGGCTGAGCT GCACCAAAGA
    GTGAATGATT TACGGGACCG CCTGTGGGAC ATTTGCGATG CCCGTAAGGA AGAAGCAGAG
    CAGGAGCGTC TGGACATCAT TAACGAGAGC TGGTTGCAGG ACTCAATGGG AATTGCCATG
    AACCATTTCT TCTCACTGAT GCAGGCAGAA CTGAACCGTT TCCAAGATAC GAAGAGATTC
    CTGCAAGATT ATTACAGGGC CATGGAATGT AAAATTCCGA TGGATGACAC TAAGAAATTC
    ACTCGAGTCC CACTGGTCCG ACTGGATAGT AGAGATATTT TGGAAAGCCA GCTTAGAATT
    CCCCTGGTTC CTCGAAGATC CATTTCTCCA GAGTCAGCTT TATCCAAACC AAAAACAAAA
    AGCATCCTGA AGGGCAAGAT CGATTACACG CTGGAAAGTG TAGAGTTAAA CTTTGAGACT
    GATGAGAAGA TGGTGATGGA TACGTGGCAG CAGTCTTCTT TGGCAGTATC TCACATGGTG
    GCTGCTGAAA TTCATCAGAG GCTCATGGAA GAAGAAAAAG AAAACCAGCC AGCAGAAACA
    AAAGAAAAAC TTCCTCAGGC AGCTGCAAAT AAAAAAGTGA AAAAGGAGCC TCCGCCACCG
    CCACCACCAC CCAAGAAAAA GAAAGAGGAC AAAAAAGGAA AAGGTAAATC CCCACCTATG
    GCAGAAGCTA CTCCTGTAGT AGTGCCAGCA GAAGAGACTG AGGAAGTGGA ATTAAAAAAT
    GAACTGAAGC TCAGAATAAA GGAAGAGCAT CTTGCTGCCC TGCAATTTGA AGAGATAGCT
    ACACAGTTCC GACTTGAACT GATAAAGGTA AAAGCATTGG CTTTCCTGGA AGAGTTAGTA
    ATAAAGGCGG TTGATGTATA TAGACTCATG GACAAGTGGC TCGGTGAGAG GTACTTGAAT
    GAAATGGCCA GTGTGGAAAA ACTAACTGGG GTAGCTCGCT ATCACATTGA AACATCAACC
    AAAATTCAGA ATGAACTTTA TTTAGACCAA GAAGACTTCT TCATTAATGG CGATATCAAA
    GTCTTTCCAG ATCCACCTCC TCCGGTACGC CCTCCGCCTG TGGAAAAGGA GGAAAATGGC
    ACCCTGACCA TGGAGCAGCT TGACAACCTC CGGGATCAGT TCTTAGATAT AGCGCCCAAA
    GGTATAATAG GAAATAAAGC ATTTGCTGAC ATTCTGCTGG ATTTGGTAAC TCTGAACCTT
    GGAACAAACA ACTTTCCTAC TAGTTGGATG AACCTCACTC AACCGGAAGC TGGCCTGTGG
    TTTACAGGAG ATGAAGATAT CAAAATTCCA GAAAATCCTC TTGAACCCCT GCCATTCAAT
    AGGCAGGAGC ATCTTATAGA GTTTTTCTTT AGGTTGTTTG CTGACTGCGA GAAGGTTCCA
    CCCCAGCTTG ACTACACACA GATGCTGCTT TATTTTGCTT GCCATCCAGA CAGCACAGAA
    GGGGTCTACA GGGCCCTCAG CGTAGCCATT GGGACTCACG TCTTCCAGCC AATTGAAATG
    CCTCATCCTG TTGCAGCAAA GACCTCTTCC TTTACTGATA TGAGTCCCAT TGAGGAATAT
    CCTGAACTCG AGGACAACTT TATGAGTGAG GAAAGGGAAT TACAAGAGGA CGGGGAGGAA
    AAGGAAGAAG AAATCCCTGA AAATGCAAAT ACGGAAATGA TTTCCATGCA AACATTACTC
    CAAGTGTTCC GAGGAGGAAA TGAAGTACTG GACGCTAACA GATTTGCCAG CCACCAAAAG
    ATAGAGAACA TTTATTTAGA GAACTTCATA AAAGTGTTTC AGGACCTAGG TTCCAAGAAC
    CTTGAGCCAA TTGAAGTTGC TGTTCTTCTG AAGCATCCTT TTATTCAAGA CCTGATTTCA
    AGTTATCCAG ACTATAAGAT CCCTGATGTT AAGATAATTC TCCAAAGGAG TGAGCATGTA
    CAAGGAAGTG ATGAAGAGAG GTCTCCTTCA AGACTTACAG AGGAAAAGAA ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OSm32427
    Clone ID Related Accession (Same CDS sequence) XM_012097081.2 , XM_012097080.2
    Accession Version XM_012097081.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5988bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-16
    Organism Ovis aries(sheep)
    Product sperm flagellar protein 2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014639025.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012097081.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    ATGCCAACTG TACAGCCCCT GATGAGCACA AAAGTTCAAA ACATGAAAAG CGAGGCTTTC 
    CGAGATCAAC GCTTGAGCAA AAGACGACAA AATGAAATCA TGGCCAAAAT CCAAGCAGCT
    ATCATACAGA TTCCTAAACC CACATCAAAT CGTACTCTGA AAACATTTGA CGCCCAAAAA
    TTAATGAAAA AGAAAAAAGA GGCAGAGGAT GTGGCCAATG AAATTAAGAA GTTTGAAGCA
    TTAATAAAAA AGGATCTCCA GGCAAAAGAA AGTGCATCCA AGACTTCTCT AGATACAGCA
    GGCCAGACAA CCGCTGATTT GTTAAACACT TATTCAGATG ATGACTACAT TAAAAAAATC
    CAAAAACGCC TTGAAGAAGA TGCTTTTGCA CGAGAGCAAA GGGAGAAAAG ACGGCGGAGA
    CTCTTAATGG ATCAGTTAAT AGCTCATGAA GCCCAAGAGG AGGCTTATCG GGAGGAGCAG
    TTGATCAACC GGCTGATGCG ACAGTCCCAG CAGGAACGCA GGATCGCTGT GCAGCTCATG
    CACGTTAGGC ATGAAAAGGA AGTTTTATGG CAAAACAGAA TTTTCAGAGA AAAACAATAT
    GAGGAAAGAC GGCTTAAAGA CTTCCAGGAT GCTCTTGATC GAGAAGCGGC TTTAGCCAGA
    CAAGCCAAGA TTGATTTTGA AGAACAAGCC CTCAGAGATA AGGAAATTCA TGAGCAGATC
    GCCATAGAAA GAGCTCAAGC TCGTTACAAA AAGCATTATT CAATATGTGC AGAAATTTTG
    GATCAGATAC TTGATCTGTC CACTAAGGTG GCAGACTATC GATTGTTGAC AAATAACCTG
    ATCCCATATA AGTTGATGCA TGATTGGAAG GAACTGTTTT TCCATGGAAA ACCCATATAT
    GAGCAAGCCT CTCTTAGACA TTTACCAGCT AAGCTCTCTG CAGAACACCT TGTAGAAATG
    GAGAAAAGGA ACTTGCTAGA TAACAATGAT TATGAAGAAT ATAAGAACAT GGTTGGAGAA
    TGGGCCTTAC CAGAAGAAAT GGTTGAAAAC TTACCACCCT CCAACAATTG CATATTGGGC
    CATGTGATTC ACAGACTAAT TGAAAGTTCT CTTCCTCCTC AAGTTGAATC TACAGTACCT
    GAGTTACCTT CATTTGCTAT AAAAGGATGC TTGTTGGGGA AAACCTTTAG TGGAAAAACT
    ACTGCTCTAA AGGCTCTAGA GAAAAGCTTT CCTATTCAGG TACTTTCTAT TGACACTCTT
    GTTCAAGAAG CTATCCAAGC ATTTCATGAC AATGAAAGTA TCAGCGGGGC CCATGGACTT
    CAGAAAGCAC CTGAAGAAAA GGTTTTACCA GTTAGGCGAA AGAGTGGTAA AGAAAACCAG
    GACGTGCAAG ATGAAGCACT ACCAGAAAGA GAAGATAAAA CCAGACTTGA TACCGATGCT
    AGAAAAGCAG CAAAAGCTGA AGAAGTCGTA GCGAGGGATA GTTTCTCTGA ACTCACTGTG
    CGTGCTCAGC TTGGTGCAAA ATCAGAAATG CTGCTGAAGA AAGGAAAGAG CATATCTGAT
    ATATTGCTAG TCAGCATCAT GGTAAATGCC ATTAATCAGA TACCTATGTA TAAAGGCTGG
    ATCCTGGATG GCTTTCCAAT GACATTAAAC CAAGCAAAAC TTCTGGAGGA AGCACTCACA
    GGCTACAACA GAAACCTCAT AGAACTGGAG GGAAAGAAAT CACAAACATC CACATTAGCT
    GTTGACCCTA CAGCTTCCAA AGAAGTGCCT CTTCCCCCTT CTGCATTTGA TTTTGTCATG
    TTATTAGACA TTTCAGATAA TTCCTCACTG AATCGCATGA ATGACATCAT GGCTAAAGCA
    TTTTCAAGTG AAGCTCCTCG TGAAGATATC AATCAGCGTG TAGCTGCTAA AAACAAAGAT
    ACAGATGAGG ACCAGAATTT AAGAGATCAA ATACAGCACA GAATTGTAGG CTTCCTGGAC
    AACTGGCCTT CGTTGGAGCA GTGGTTTACA GAGCCAGAAA ACATTTTGAT AAAAATCAAT
    GCTGAAGTAG ATAAACAGTC ATTGTTCCAA AAAGTAAAGG AAATGATTAT GACTGAAATA
    ATTAAAAAAG AAAATAAAGT GAAAAAGAAA TTAGAAGAAA AGGAAGCTGA GAGAAAAGAA
    GAAGTTTCCC TGAGTGAGAA GACATCCCCT CCTCCTCCTC CCCCTCCGGA ACCAGAGAAA
    GAGAAGGAAG TCCACCTTCC ACAGGAGCGG TCCAAAACTC CTGCTGCAAA AGGAAAGCAA
    GCATCAGAAC CCCTGCATGG TAATCGAGAA CCTCTTCAGG AAGGAAAAGG GAAGAAAAGT
    GAAACTTCAC TCAAAAGAAA AGGTTCTCCT AGAGGAAAAT CGCTAGGAGG GAAGATTCCA
    CTCAAGAAAT CACCTGCCGA GTCTACAGAT ATTTCACCGG TCCCGGTAGT GCCAGCCCCA
    TCGAAGCCCG GATCAGAAGA GTGGGTGTAT GTAAATGAAC CAATTCCTGA GGAAATACCA
    TCATTTTTAG TACCATACTG GAAATTGATA GAAAACTCCT ATATAAATTC CATCAAAACA
    GTACTCAGGG AGTTGAGAGA AGGCCAGCAT AGTGTACTTG CTTACTTATA TGATATTAGA
    ACAGGCTTCC AGCAGTTTCT GAAGCGTCCA GACCACAAGC AAGACTTCGT GTCTCAGTGG
    CAGGCGGATT TCAACTCCCT TCCTGATGAC CTGTGGGATG ATGAGGAAAC AAAGGCTGAG
    CTGCACCAAA GAGTGAATGA TTTACGGGAC CGCCTGTGGG ACATTTGCGA TGCCCGTAAG
    GAAGAAGCAG AGCAGGAGCG TCTGGACATC ATTAACGAGA GCTGGTTGCA GGACTCAATG
    GGAATTGCCA TGAACCATTT CTTCTCACTG ATGCAGGCAG AACTGAACCG TTTCCAAGAT
    ACGAAGAGAT TCCTGCAAGA TTATTACAGG GCCATGGAAT GTAAAATTCC GATGGATGAC
    ACTAAGAAAT TCACTCGAGT CCCACTGGTC CGACTGGATA GTAGAGATAT TTTGGAAAGC
    CAGCTTAGAA TTCCCCTGGT TCCTCGAAGA TCCATTTCTC CAGAGTCAGC TTTATCCAAA
    CCAAAAACAA AAAGCATCCT GAAGGGCAAG ATCGATTACA CGCTGGAAAG TGTAGAGTTA
    AACTTTGAGA CTGATGAGAA GATGGTGATG GATACGTGGC AGCAGTCTTC TTTGGCAGTA
    TCTCACATGG TGGCTGCTGA AATTCATCAG AGGCTCATGG AAGAAGAAAA AGAAAACCAG
    CCAGCAGAAA CAAAAGAAAA ACTTCCTCAG GCAGCTGCAA ATAAAAAAGT GAAAAAGGAG
    CCTCCGCCAC CGCCACCACC ACCCAAGAAA AAGAAAGAGG ACAAAAAAGG AAAAGGTAAA
    TCCCCACCTA TGGCAGAAGC TACTCCTGTA GTAGTGCCAG CAGAAGAGAC TGAGGAAGTG
    GAATTAAAAA ATGAACTGAA GCTCAGAATA AAGGAAGAGC ATCTTGCTGC CCTGCAATTT
    GAAGAGATAG CTACACAGTT CCGACTTGAA CTGATAAAGG TAAAAGCATT GGCTTTCCTG
    GAAGAGTTAG TAATAAAGGC GGTTGATGTA TATAGACTCA TGGACAAGTG GCTCGGTGAG
    AGGTACTTGA ATGAAATGGC CAGTGTGGAA AAACTAACTG GGGTAGCTCG CTATCACATT
    GAAACATCAA CCAAAATTCA GAATGAACTT TATTTAGACC AAGAAGACTT CTTCATTAAT
    GGCGATATCA AAGTCTTTCC AGATCCACCT CCTCCGGTAC GCCCTCCGCC TGTGGAAAAG
    GAGGAAAATG GCACCCTGAC CATGGAGCAG CTTGACAACC TCCGGGATCA GTTCTTAGAT
    ATAGCGCCCA AAGGCAGGGA TCCTCCAGAC AATGTGAGTG GATTCACCAA GAAGCAGAAA
    AGGCCTCAGA AGCTTGACCA TTACCTCCCA AAGAGGAACA ATCAATTTTT GAAAATTTGG
    CTTAAAAAAT ACCCTTGGCT TGTATACGAT GAGATACTAA ATCTTATGTT CTGCGGCCTG
    TGTCGTAAAC ATGGAGTGAC ATCAAAGGGA AATCAGGTCA GTTTTTTTTA CGGCACGGAT
    AACTTCAGGA CTGAATTTCT CAACGCGCAT CATCTGAGTG AGGCTCACGC CAAGGCGTCC
    CTCATGGAAG CCACAAGTGG TTCTCCAGGG AGCAGAGCTA CCACGGAGCT GATGGTGAGG
    ACCATGAGCA AGGTGACCCT TGGCAGGGTG GAGAACCTGT TCCGGTCCTG CCACGCCATC
    GCCAAGACCG GACGTCCCCT CAAAGACTTC CTCTGGATGT GTTCGCTGGA CGATATGAAG
    GGAATTGATA TCGGCCCGGT GTTCAGAACC AGTAAATCGG CCAGAACTTT TACCTACTTT
    ATTGCTGAAG TGGAACGGAG AACTCTAAGA GAGAAGTTAG AAAAAAGTCA GTTCTTCTCC
    GTCCTCAGTG ATGGGATTGC AGACAATTCA GCTGAGGAAG CTGAACTCGT ATATGTGCAG
    TTTGCCCGCG CAGGGAGAGT GCACTGCCAA ATCGTCGGGG TACAGACCGT GGAGAAGAAG
    GAGCCCTGGG CGATAAAACG TGCCATTGAG AAAACTCTGG AGAGAAATCT TCAACTTCGA
    CTGTCGAACC AAGACTGGGC AAAGAAACTG GTGGGTTTCG GAAGCGATGG AGCTCCAGGT
    TCAGAGGGAG AAAACGGCAG CACAGTGGCC CTGCTTTTAA GGGAAATCCA GCCAGGTGTG
    CTGCCTGTTT ACTGCCTTGC CCATCACCTC GAACTGTCTT ACCAGGCGGT GTTCCAGAGC
    GTTCCCCTCT ACAGCCGTGT GAAAGACCTT CTTCATAGCA TGCACCGCTT CTATCACGAT
    TCTCCTGCTC ACAGGAGTGC TCTGGTGACT GCTTTCAAGG GCCTCCACCT TCAACCTGCG
    ATGCCCTCTC AAGTCAGAGG CCGGAGGTGG CTTCAGGGAT TGCAGGCTGC CCTCCAGAAC
    TTTCTCAAGG GATATCCAGC AATTGTCCAG CAGCTGCAGT CAACAAAAGA AAGCAGAAGC
    AACAGCAGTC ATCAGAAAGC CGAAGAACTC CTGGAGTCTC TCCTCCAGGC AGACATTGTC
    AAATTTGTCC ACTTCTTGGT GGATGTCGTC AATGTCCTTA GCATACTGTC CCATGTCAAT
    CAGAACAGAA ATTCCTCAAT TGCTGATGTA TTTGCCACCT TAGAGTCAAC ACTGGAAATG
    CTCCAAATTT ATCAGACAAG ACCAGGGCCG AAAGAACGTG GGGTAGATTC GGTCACACAC
    TTTCATGGTA ACTGCCTTGG AGGAAAGGGA AACATTTCTG ACGTGAGAAA CATTGTTTTA
    ACACATCTTA TCAAGAGGCT GCGAGGCTGT TTCAGAGATG CCAACCAAGA TGTGGTGAAG
    GCAACCACGA TTGGAAGCTT TAAACTGTGG CCGGCAAAAA TAAATCAAGA ATTTGGAGAA
    AAAGAGGTGG CCATCCTGAT TGCACATTAT GAACCAGTAT TAGAGGCAGC CAAGGTGAAA
    ATTGACCAAG TAGACACAGA ATGGAGCATG TTGAAATTGG AGATCTACTC TAGATTTCAG
    AACATTTGCA AACTGACCTG GGATTTTGTG AATTCTGTTT ATTTACACAA GTATCCAAAT
    ATCCTGACAC TCATTGACCT CGTGCTGAGC CTGCCTGCAA GTTCAGCTGA GGCAGGACGT
    GGCTTTAGTC AGATGAAGCG CATCAGGTCC CAAATGCATG CCAAGGTCAA GGCTGACAAC
    ATGACGGATC TCCTGATAAT TCAGTTGAAT TCTCCAGACA TTAACAACTT TGACCCTAGG
    AAAGCCATTC ATTTATGGAA TACAAGAACA CCATCCTCAA CTGAGGACAC AAGACCTAAC
    TCAGACTGCT CATCAAACTC AGAAAGCCTT AGTGAAAGCG ATGAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011952471.1
    CDS739..6726
    Translation

    Target ORF information:

    RefSeq Version XM_012097081.2
    Organism Ovis aries(sheep)
    Definition Ovis aries sperm flagellar 2 (SPEF2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012097081.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    ATGCCAACTG TACAGCCCCT GATGAGCACA AAAGTTCAAA ACATGAAAAG CGAGGCTTTC 
    CGAGATCAAC GCTTGAGCAA AAGACGACAA AATGAAATCA TGGCCAAAAT CCAAGCAGCT
    ATCATACAGA TTCCTAAACC CACATCAAAT CGTACTCTGA AAACATTTGA CGCCCAAAAA
    TTAATGAAAA AGAAAAAAGA GGCAGAGGAT GTGGCCAATG AAATTAAGAA GTTTGAAGCA
    TTAATAAAAA AGGATCTCCA GGCAAAAGAA AGTGCATCCA AGACTTCTCT AGATACAGCA
    GGCCAGACAA CCGCTGATTT GTTAAACACT TATTCAGATG ATGACTACAT TAAAAAAATC
    CAAAAACGCC TTGAAGAAGA TGCTTTTGCA CGAGAGCAAA GGGAGAAAAG ACGGCGGAGA
    CTCTTAATGG ATCAGTTAAT AGCTCATGAA GCCCAAGAGG AGGCTTATCG GGAGGAGCAG
    TTGATCAACC GGCTGATGCG ACAGTCCCAG CAGGAACGCA GGATCGCTGT GCAGCTCATG
    CACGTTAGGC ATGAAAAGGA AGTTTTATGG CAAAACAGAA TTTTCAGAGA AAAACAATAT
    GAGGAAAGAC GGCTTAAAGA CTTCCAGGAT GCTCTTGATC GAGAAGCGGC TTTAGCCAGA
    CAAGCCAAGA TTGATTTTGA AGAACAAGCC CTCAGAGATA AGGAAATTCA TGAGCAGATC
    GCCATAGAAA GAGCTCAAGC TCGTTACAAA AAGCATTATT CAATATGTGC AGAAATTTTG
    GATCAGATAC TTGATCTGTC CACTAAGGTG GCAGACTATC GATTGTTGAC AAATAACCTG
    ATCCCATATA AGTTGATGCA TGATTGGAAG GAACTGTTTT TCCATGGAAA ACCCATATAT
    GAGCAAGCCT CTCTTAGACA TTTACCAGCT AAGCTCTCTG CAGAACACCT TGTAGAAATG
    GAGAAAAGGA ACTTGCTAGA TAACAATGAT TATGAAGAAT ATAAGAACAT GGTTGGAGAA
    TGGGCCTTAC CAGAAGAAAT GGTTGAAAAC TTACCACCCT CCAACAATTG CATATTGGGC
    CATGTGATTC ACAGACTAAT TGAAAGTTCT CTTCCTCCTC AAGTTGAATC TACAGTACCT
    GAGTTACCTT CATTTGCTAT AAAAGGATGC TTGTTGGGGA AAACCTTTAG TGGAAAAACT
    ACTGCTCTAA AGGCTCTAGA GAAAAGCTTT CCTATTCAGG TACTTTCTAT TGACACTCTT
    GTTCAAGAAG CTATCCAAGC ATTTCATGAC AATGAAAGTA TCAGCGGGGC CCATGGACTT
    CAGAAAGCAC CTGAAGAAAA GGTTTTACCA GTTAGGCGAA AGAGTGGTAA AGAAAACCAG
    GACGTGCAAG ATGAAGCACT ACCAGAAAGA GAAGATAAAA CCAGACTTGA TACCGATGCT
    AGAAAAGCAG CAAAAGCTGA AGAAGTCGTA GCGAGGGATA GTTTCTCTGA ACTCACTGTG
    CGTGCTCAGC TTGGTGCAAA ATCAGAAATG CTGCTGAAGA AAGGAAAGAG CATATCTGAT
    ATATTGCTAG TCAGCATCAT GGTAAATGCC ATTAATCAGA TACCTATGTA TAAAGGCTGG
    ATCCTGGATG GCTTTCCAAT GACATTAAAC CAAGCAAAAC TTCTGGAGGA AGCACTCACA
    GGCTACAACA GAAACCTCAT AGAACTGGAG GGAAAGAAAT CACAAACATC CACATTAGCT
    GTTGACCCTA CAGCTTCCAA AGAAGTGCCT CTTCCCCCTT CTGCATTTGA TTTTGTCATG
    TTATTAGACA TTTCAGATAA TTCCTCACTG AATCGCATGA ATGACATCAT GGCTAAAGCA
    TTTTCAAGTG AAGCTCCTCG TGAAGATATC AATCAGCGTG TAGCTGCTAA AAACAAAGAT
    ACAGATGAGG ACCAGAATTT AAGAGATCAA ATACAGCACA GAATTGTAGG CTTCCTGGAC
    AACTGGCCTT CGTTGGAGCA GTGGTTTACA GAGCCAGAAA ACATTTTGAT AAAAATCAAT
    GCTGAAGTAG ATAAACAGTC ATTGTTCCAA AAAGTAAAGG AAATGATTAT GACTGAAATA
    ATTAAAAAAG AAAATAAAGT GAAAAAGAAA TTAGAAGAAA AGGAAGCTGA GAGAAAAGAA
    GAAGTTTCCC TGAGTGAGAA GACATCCCCT CCTCCTCCTC CCCCTCCGGA ACCAGAGAAA
    GAGAAGGAAG TCCACCTTCC ACAGGAGCGG TCCAAAACTC CTGCTGCAAA AGGAAAGCAA
    GCATCAGAAC CCCTGCATGG TAATCGAGAA CCTCTTCAGG AAGGAAAAGG GAAGAAAAGT
    GAAACTTCAC TCAAAAGAAA AGGTTCTCCT AGAGGAAAAT CGCTAGGAGG GAAGATTCCA
    CTCAAGAAAT CACCTGCCGA GTCTACAGAT ATTTCACCGG TCCCGGTAGT GCCAGCCCCA
    TCGAAGCCCG GATCAGAAGA GTGGGTGTAT GTAAATGAAC CAATTCCTGA GGAAATACCA
    TCATTTTTAG TACCATACTG GAAATTGATA GAAAACTCCT ATATAAATTC CATCAAAACA
    GTACTCAGGG AGTTGAGAGA AGGCCAGCAT AGTGTACTTG CTTACTTATA TGATATTAGA
    ACAGGCTTCC AGCAGTTTCT GAAGCGTCCA GACCACAAGC AAGACTTCGT GTCTCAGTGG
    CAGGCGGATT TCAACTCCCT TCCTGATGAC CTGTGGGATG ATGAGGAAAC AAAGGCTGAG
    CTGCACCAAA GAGTGAATGA TTTACGGGAC CGCCTGTGGG ACATTTGCGA TGCCCGTAAG
    GAAGAAGCAG AGCAGGAGCG TCTGGACATC ATTAACGAGA GCTGGTTGCA GGACTCAATG
    GGAATTGCCA TGAACCATTT CTTCTCACTG ATGCAGGCAG AACTGAACCG TTTCCAAGAT
    ACGAAGAGAT TCCTGCAAGA TTATTACAGG GCCATGGAAT GTAAAATTCC GATGGATGAC
    ACTAAGAAAT TCACTCGAGT CCCACTGGTC CGACTGGATA GTAGAGATAT TTTGGAAAGC
    CAGCTTAGAA TTCCCCTGGT TCCTCGAAGA TCCATTTCTC CAGAGTCAGC TTTATCCAAA
    CCAAAAACAA AAAGCATCCT GAAGGGCAAG ATCGATTACA CGCTGGAAAG TGTAGAGTTA
    AACTTTGAGA CTGATGAGAA GATGGTGATG GATACGTGGC AGCAGTCTTC TTTGGCAGTA
    TCTCACATGG TGGCTGCTGA AATTCATCAG AGGCTCATGG AAGAAGAAAA AGAAAACCAG
    CCAGCAGAAA CAAAAGAAAA ACTTCCTCAG GCAGCTGCAA ATAAAAAAGT GAAAAAGGAG
    CCTCCGCCAC CGCCACCACC ACCCAAGAAA AAGAAAGAGG ACAAAAAAGG AAAAGGTAAA
    TCCCCACCTA TGGCAGAAGC TACTCCTGTA GTAGTGCCAG CAGAAGAGAC TGAGGAAGTG
    GAATTAAAAA ATGAACTGAA GCTCAGAATA AAGGAAGAGC ATCTTGCTGC CCTGCAATTT
    GAAGAGATAG CTACACAGTT CCGACTTGAA CTGATAAAGG TAAAAGCATT GGCTTTCCTG
    GAAGAGTTAG TAATAAAGGC GGTTGATGTA TATAGACTCA TGGACAAGTG GCTCGGTGAG
    AGGTACTTGA ATGAAATGGC CAGTGTGGAA AAACTAACTG GGGTAGCTCG CTATCACATT
    GAAACATCAA CCAAAATTCA GAATGAACTT TATTTAGACC AAGAAGACTT CTTCATTAAT
    GGCGATATCA AAGTCTTTCC AGATCCACCT CCTCCGGTAC GCCCTCCGCC TGTGGAAAAG
    GAGGAAAATG GCACCCTGAC CATGGAGCAG CTTGACAACC TCCGGGATCA GTTCTTAGAT
    ATAGCGCCCA AAGGCAGGGA TCCTCCAGAC AATGTGAGTG GATTCACCAA GAAGCAGAAA
    AGGCCTCAGA AGCTTGACCA TTACCTCCCA AAGAGGAACA ATCAATTTTT GAAAATTTGG
    CTTAAAAAAT ACCCTTGGCT TGTATACGAT GAGATACTAA ATCTTATGTT CTGCGGCCTG
    TGTCGTAAAC ATGGAGTGAC ATCAAAGGGA AATCAGGTCA GTTTTTTTTA CGGCACGGAT
    AACTTCAGGA CTGAATTTCT CAACGCGCAT CATCTGAGTG AGGCTCACGC CAAGGCGTCC
    CTCATGGAAG CCACAAGTGG TTCTCCAGGG AGCAGAGCTA CCACGGAGCT GATGGTGAGG
    ACCATGAGCA AGGTGACCCT TGGCAGGGTG GAGAACCTGT TCCGGTCCTG CCACGCCATC
    GCCAAGACCG GACGTCCCCT CAAAGACTTC CTCTGGATGT GTTCGCTGGA CGATATGAAG
    GGAATTGATA TCGGCCCGGT GTTCAGAACC AGTAAATCGG CCAGAACTTT TACCTACTTT
    ATTGCTGAAG TGGAACGGAG AACTCTAAGA GAGAAGTTAG AAAAAAGTCA GTTCTTCTCC
    GTCCTCAGTG ATGGGATTGC AGACAATTCA GCTGAGGAAG CTGAACTCGT ATATGTGCAG
    TTTGCCCGCG CAGGGAGAGT GCACTGCCAA ATCGTCGGGG TACAGACCGT GGAGAAGAAG
    GAGCCCTGGG CGATAAAACG TGCCATTGAG AAAACTCTGG AGAGAAATCT TCAACTTCGA
    CTGTCGAACC AAGACTGGGC AAAGAAACTG GTGGGTTTCG GAAGCGATGG AGCTCCAGGT
    TCAGAGGGAG AAAACGGCAG CACAGTGGCC CTGCTTTTAA GGGAAATCCA GCCAGGTGTG
    CTGCCTGTTT ACTGCCTTGC CCATCACCTC GAACTGTCTT ACCAGGCGGT GTTCCAGAGC
    GTTCCCCTCT ACAGCCGTGT GAAAGACCTT CTTCATAGCA TGCACCGCTT CTATCACGAT
    TCTCCTGCTC ACAGGAGTGC TCTGGTGACT GCTTTCAAGG GCCTCCACCT TCAACCTGCG
    ATGCCCTCTC AAGTCAGAGG CCGGAGGTGG CTTCAGGGAT TGCAGGCTGC CCTCCAGAAC
    TTTCTCAAGG GATATCCAGC AATTGTCCAG CAGCTGCAGT CAACAAAAGA AAGCAGAAGC
    AACAGCAGTC ATCAGAAAGC CGAAGAACTC CTGGAGTCTC TCCTCCAGGC AGACATTGTC
    AAATTTGTCC ACTTCTTGGT GGATGTCGTC AATGTCCTTA GCATACTGTC CCATGTCAAT
    CAGAACAGAA ATTCCTCAAT TGCTGATGTA TTTGCCACCT TAGAGTCAAC ACTGGAAATG
    CTCCAAATTT ATCAGACAAG ACCAGGGCCG AAAGAACGTG GGGTAGATTC GGTCACACAC
    TTTCATGGTA ACTGCCTTGG AGGAAAGGGA AACATTTCTG ACGTGAGAAA CATTGTTTTA
    ACACATCTTA TCAAGAGGCT GCGAGGCTGT TTCAGAGATG CCAACCAAGA TGTGGTGAAG
    GCAACCACGA TTGGAAGCTT TAAACTGTGG CCGGCAAAAA TAAATCAAGA ATTTGGAGAA
    AAAGAGGTGG CCATCCTGAT TGCACATTAT GAACCAGTAT TAGAGGCAGC CAAGGTGAAA
    ATTGACCAAG TAGACACAGA ATGGAGCATG TTGAAATTGG AGATCTACTC TAGATTTCAG
    AACATTTGCA AACTGACCTG GGATTTTGTG AATTCTGTTT ATTTACACAA GTATCCAAAT
    ATCCTGACAC TCATTGACCT CGTGCTGAGC CTGCCTGCAA GTTCAGCTGA GGCAGGACGT
    GGCTTTAGTC AGATGAAGCG CATCAGGTCC CAAATGCATG CCAAGGTCAA GGCTGACAAC
    ATGACGGATC TCCTGATAAT TCAGTTGAAT TCTCCAGACA TTAACAACTT TGACCCTAGG
    AAAGCCATTC ATTTATGGAA TACAAGAACA CCATCCTCAA CTGAGGACAC AAGACCTAAC
    TCAGACTGCT CATCAAACTC AGAAAGCCTT AGTGAAAGCG ATGAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OSm32427
    Clone ID Related Accession (Same CDS sequence) XM_012097081.2 , XM_012097080.2
    Accession Version XM_012097080.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5988bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-16
    Organism Ovis aries(sheep)
    Product sperm flagellar protein 2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014639025.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012097080.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    ATGCCAACTG TACAGCCCCT GATGAGCACA AAAGTTCAAA ACATGAAAAG CGAGGCTTTC 
    CGAGATCAAC GCTTGAGCAA AAGACGACAA AATGAAATCA TGGCCAAAAT CCAAGCAGCT
    ATCATACAGA TTCCTAAACC CACATCAAAT CGTACTCTGA AAACATTTGA CGCCCAAAAA
    TTAATGAAAA AGAAAAAAGA GGCAGAGGAT GTGGCCAATG AAATTAAGAA GTTTGAAGCA
    TTAATAAAAA AGGATCTCCA GGCAAAAGAA AGTGCATCCA AGACTTCTCT AGATACAGCA
    GGCCAGACAA CCGCTGATTT GTTAAACACT TATTCAGATG ATGACTACAT TAAAAAAATC
    CAAAAACGCC TTGAAGAAGA TGCTTTTGCA CGAGAGCAAA GGGAGAAAAG ACGGCGGAGA
    CTCTTAATGG ATCAGTTAAT AGCTCATGAA GCCCAAGAGG AGGCTTATCG GGAGGAGCAG
    TTGATCAACC GGCTGATGCG ACAGTCCCAG CAGGAACGCA GGATCGCTGT GCAGCTCATG
    CACGTTAGGC ATGAAAAGGA AGTTTTATGG CAAAACAGAA TTTTCAGAGA AAAACAATAT
    GAGGAAAGAC GGCTTAAAGA CTTCCAGGAT GCTCTTGATC GAGAAGCGGC TTTAGCCAGA
    CAAGCCAAGA TTGATTTTGA AGAACAAGCC CTCAGAGATA AGGAAATTCA TGAGCAGATC
    GCCATAGAAA GAGCTCAAGC TCGTTACAAA AAGCATTATT CAATATGTGC AGAAATTTTG
    GATCAGATAC TTGATCTGTC CACTAAGGTG GCAGACTATC GATTGTTGAC AAATAACCTG
    ATCCCATATA AGTTGATGCA TGATTGGAAG GAACTGTTTT TCCATGGAAA ACCCATATAT
    GAGCAAGCCT CTCTTAGACA TTTACCAGCT AAGCTCTCTG CAGAACACCT TGTAGAAATG
    GAGAAAAGGA ACTTGCTAGA TAACAATGAT TATGAAGAAT ATAAGAACAT GGTTGGAGAA
    TGGGCCTTAC CAGAAGAAAT GGTTGAAAAC TTACCACCCT CCAACAATTG CATATTGGGC
    CATGTGATTC ACAGACTAAT TGAAAGTTCT CTTCCTCCTC AAGTTGAATC TACAGTACCT
    GAGTTACCTT CATTTGCTAT AAAAGGATGC TTGTTGGGGA AAACCTTTAG TGGAAAAACT
    ACTGCTCTAA AGGCTCTAGA GAAAAGCTTT CCTATTCAGG TACTTTCTAT TGACACTCTT
    GTTCAAGAAG CTATCCAAGC ATTTCATGAC AATGAAAGTA TCAGCGGGGC CCATGGACTT
    CAGAAAGCAC CTGAAGAAAA GGTTTTACCA GTTAGGCGAA AGAGTGGTAA AGAAAACCAG
    GACGTGCAAG ATGAAGCACT ACCAGAAAGA GAAGATAAAA CCAGACTTGA TACCGATGCT
    AGAAAAGCAG CAAAAGCTGA AGAAGTCGTA GCGAGGGATA GTTTCTCTGA ACTCACTGTG
    CGTGCTCAGC TTGGTGCAAA ATCAGAAATG CTGCTGAAGA AAGGAAAGAG CATATCTGAT
    ATATTGCTAG TCAGCATCAT GGTAAATGCC ATTAATCAGA TACCTATGTA TAAAGGCTGG
    ATCCTGGATG GCTTTCCAAT GACATTAAAC CAAGCAAAAC TTCTGGAGGA AGCACTCACA
    GGCTACAACA GAAACCTCAT AGAACTGGAG GGAAAGAAAT CACAAACATC CACATTAGCT
    GTTGACCCTA CAGCTTCCAA AGAAGTGCCT CTTCCCCCTT CTGCATTTGA TTTTGTCATG
    TTATTAGACA TTTCAGATAA TTCCTCACTG AATCGCATGA ATGACATCAT GGCTAAAGCA
    TTTTCAAGTG AAGCTCCTCG TGAAGATATC AATCAGCGTG TAGCTGCTAA AAACAAAGAT
    ACAGATGAGG ACCAGAATTT AAGAGATCAA ATACAGCACA GAATTGTAGG CTTCCTGGAC
    AACTGGCCTT CGTTGGAGCA GTGGTTTACA GAGCCAGAAA ACATTTTGAT AAAAATCAAT
    GCTGAAGTAG ATAAACAGTC ATTGTTCCAA AAAGTAAAGG AAATGATTAT GACTGAAATA
    ATTAAAAAAG AAAATAAAGT GAAAAAGAAA TTAGAAGAAA AGGAAGCTGA GAGAAAAGAA
    GAAGTTTCCC TGAGTGAGAA GACATCCCCT CCTCCTCCTC CCCCTCCGGA ACCAGAGAAA
    GAGAAGGAAG TCCACCTTCC ACAGGAGCGG TCCAAAACTC CTGCTGCAAA AGGAAAGCAA
    GCATCAGAAC CCCTGCATGG TAATCGAGAA CCTCTTCAGG AAGGAAAAGG GAAGAAAAGT
    GAAACTTCAC TCAAAAGAAA AGGTTCTCCT AGAGGAAAAT CGCTAGGAGG GAAGATTCCA
    CTCAAGAAAT CACCTGCCGA GTCTACAGAT ATTTCACCGG TCCCGGTAGT GCCAGCCCCA
    TCGAAGCCCG GATCAGAAGA GTGGGTGTAT GTAAATGAAC CAATTCCTGA GGAAATACCA
    TCATTTTTAG TACCATACTG GAAATTGATA GAAAACTCCT ATATAAATTC CATCAAAACA
    GTACTCAGGG AGTTGAGAGA AGGCCAGCAT AGTGTACTTG CTTACTTATA TGATATTAGA
    ACAGGCTTCC AGCAGTTTCT GAAGCGTCCA GACCACAAGC AAGACTTCGT GTCTCAGTGG
    CAGGCGGATT TCAACTCCCT TCCTGATGAC CTGTGGGATG ATGAGGAAAC AAAGGCTGAG
    CTGCACCAAA GAGTGAATGA TTTACGGGAC CGCCTGTGGG ACATTTGCGA TGCCCGTAAG
    GAAGAAGCAG AGCAGGAGCG TCTGGACATC ATTAACGAGA GCTGGTTGCA GGACTCAATG
    GGAATTGCCA TGAACCATTT CTTCTCACTG ATGCAGGCAG AACTGAACCG TTTCCAAGAT
    ACGAAGAGAT TCCTGCAAGA TTATTACAGG GCCATGGAAT GTAAAATTCC GATGGATGAC
    ACTAAGAAAT TCACTCGAGT CCCACTGGTC CGACTGGATA GTAGAGATAT TTTGGAAAGC
    CAGCTTAGAA TTCCCCTGGT TCCTCGAAGA TCCATTTCTC CAGAGTCAGC TTTATCCAAA
    CCAAAAACAA AAAGCATCCT GAAGGGCAAG ATCGATTACA CGCTGGAAAG TGTAGAGTTA
    AACTTTGAGA CTGATGAGAA GATGGTGATG GATACGTGGC AGCAGTCTTC TTTGGCAGTA
    TCTCACATGG TGGCTGCTGA AATTCATCAG AGGCTCATGG AAGAAGAAAA AGAAAACCAG
    CCAGCAGAAA CAAAAGAAAA ACTTCCTCAG GCAGCTGCAA ATAAAAAAGT GAAAAAGGAG
    CCTCCGCCAC CGCCACCACC ACCCAAGAAA AAGAAAGAGG ACAAAAAAGG AAAAGGTAAA
    TCCCCACCTA TGGCAGAAGC TACTCCTGTA GTAGTGCCAG CAGAAGAGAC TGAGGAAGTG
    GAATTAAAAA ATGAACTGAA GCTCAGAATA AAGGAAGAGC ATCTTGCTGC CCTGCAATTT
    GAAGAGATAG CTACACAGTT CCGACTTGAA CTGATAAAGG TAAAAGCATT GGCTTTCCTG
    GAAGAGTTAG TAATAAAGGC GGTTGATGTA TATAGACTCA TGGACAAGTG GCTCGGTGAG
    AGGTACTTGA ATGAAATGGC CAGTGTGGAA AAACTAACTG GGGTAGCTCG CTATCACATT
    GAAACATCAA CCAAAATTCA GAATGAACTT TATTTAGACC AAGAAGACTT CTTCATTAAT
    GGCGATATCA AAGTCTTTCC AGATCCACCT CCTCCGGTAC GCCCTCCGCC TGTGGAAAAG
    GAGGAAAATG GCACCCTGAC CATGGAGCAG CTTGACAACC TCCGGGATCA GTTCTTAGAT
    ATAGCGCCCA AAGGCAGGGA TCCTCCAGAC AATGTGAGTG GATTCACCAA GAAGCAGAAA
    AGGCCTCAGA AGCTTGACCA TTACCTCCCA AAGAGGAACA ATCAATTTTT GAAAATTTGG
    CTTAAAAAAT ACCCTTGGCT TGTATACGAT GAGATACTAA ATCTTATGTT CTGCGGCCTG
    TGTCGTAAAC ATGGAGTGAC ATCAAAGGGA AATCAGGTCA GTTTTTTTTA CGGCACGGAT
    AACTTCAGGA CTGAATTTCT CAACGCGCAT CATCTGAGTG AGGCTCACGC CAAGGCGTCC
    CTCATGGAAG CCACAAGTGG TTCTCCAGGG AGCAGAGCTA CCACGGAGCT GATGGTGAGG
    ACCATGAGCA AGGTGACCCT TGGCAGGGTG GAGAACCTGT TCCGGTCCTG CCACGCCATC
    GCCAAGACCG GACGTCCCCT CAAAGACTTC CTCTGGATGT GTTCGCTGGA CGATATGAAG
    GGAATTGATA TCGGCCCGGT GTTCAGAACC AGTAAATCGG CCAGAACTTT TACCTACTTT
    ATTGCTGAAG TGGAACGGAG AACTCTAAGA GAGAAGTTAG AAAAAAGTCA GTTCTTCTCC
    GTCCTCAGTG ATGGGATTGC AGACAATTCA GCTGAGGAAG CTGAACTCGT ATATGTGCAG
    TTTGCCCGCG CAGGGAGAGT GCACTGCCAA ATCGTCGGGG TACAGACCGT GGAGAAGAAG
    GAGCCCTGGG CGATAAAACG TGCCATTGAG AAAACTCTGG AGAGAAATCT TCAACTTCGA
    CTGTCGAACC AAGACTGGGC AAAGAAACTG GTGGGTTTCG GAAGCGATGG AGCTCCAGGT
    TCAGAGGGAG AAAACGGCAG CACAGTGGCC CTGCTTTTAA GGGAAATCCA GCCAGGTGTG
    CTGCCTGTTT ACTGCCTTGC CCATCACCTC GAACTGTCTT ACCAGGCGGT GTTCCAGAGC
    GTTCCCCTCT ACAGCCGTGT GAAAGACCTT CTTCATAGCA TGCACCGCTT CTATCACGAT
    TCTCCTGCTC ACAGGAGTGC TCTGGTGACT GCTTTCAAGG GCCTCCACCT TCAACCTGCG
    ATGCCCTCTC AAGTCAGAGG CCGGAGGTGG CTTCAGGGAT TGCAGGCTGC CCTCCAGAAC
    TTTCTCAAGG GATATCCAGC AATTGTCCAG CAGCTGCAGT CAACAAAAGA AAGCAGAAGC
    AACAGCAGTC ATCAGAAAGC CGAAGAACTC CTGGAGTCTC TCCTCCAGGC AGACATTGTC
    AAATTTGTCC ACTTCTTGGT GGATGTCGTC AATGTCCTTA GCATACTGTC CCATGTCAAT
    CAGAACAGAA ATTCCTCAAT TGCTGATGTA TTTGCCACCT TAGAGTCAAC ACTGGAAATG
    CTCCAAATTT ATCAGACAAG ACCAGGGCCG AAAGAACGTG GGGTAGATTC GGTCACACAC
    TTTCATGGTA ACTGCCTTGG AGGAAAGGGA AACATTTCTG ACGTGAGAAA CATTGTTTTA
    ACACATCTTA TCAAGAGGCT GCGAGGCTGT TTCAGAGATG CCAACCAAGA TGTGGTGAAG
    GCAACCACGA TTGGAAGCTT TAAACTGTGG CCGGCAAAAA TAAATCAAGA ATTTGGAGAA
    AAAGAGGTGG CCATCCTGAT TGCACATTAT GAACCAGTAT TAGAGGCAGC CAAGGTGAAA
    ATTGACCAAG TAGACACAGA ATGGAGCATG TTGAAATTGG AGATCTACTC TAGATTTCAG
    AACATTTGCA AACTGACCTG GGATTTTGTG AATTCTGTTT ATTTACACAA GTATCCAAAT
    ATCCTGACAC TCATTGACCT CGTGCTGAGC CTGCCTGCAA GTTCAGCTGA GGCAGGACGT
    GGCTTTAGTC AGATGAAGCG CATCAGGTCC CAAATGCATG CCAAGGTCAA GGCTGACAAC
    ATGACGGATC TCCTGATAAT TCAGTTGAAT TCTCCAGACA TTAACAACTT TGACCCTAGG
    AAAGCCATTC ATTTATGGAA TACAAGAACA CCATCCTCAA CTGAGGACAC AAGACCTAAC
    TCAGACTGCT CATCAAACTC AGAAAGCCTT AGTGAAAGCG ATGAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011952470.1
    CDS743..6730
    Translation

    Target ORF information:

    RefSeq Version XM_012097080.2
    Organism Ovis aries(sheep)
    Definition Ovis aries sperm flagellar 2 (SPEF2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012097080.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    ATGCCAACTG TACAGCCCCT GATGAGCACA AAAGTTCAAA ACATGAAAAG CGAGGCTTTC 
    CGAGATCAAC GCTTGAGCAA AAGACGACAA AATGAAATCA TGGCCAAAAT CCAAGCAGCT
    ATCATACAGA TTCCTAAACC CACATCAAAT CGTACTCTGA AAACATTTGA CGCCCAAAAA
    TTAATGAAAA AGAAAAAAGA GGCAGAGGAT GTGGCCAATG AAATTAAGAA GTTTGAAGCA
    TTAATAAAAA AGGATCTCCA GGCAAAAGAA AGTGCATCCA AGACTTCTCT AGATACAGCA
    GGCCAGACAA CCGCTGATTT GTTAAACACT TATTCAGATG ATGACTACAT TAAAAAAATC
    CAAAAACGCC TTGAAGAAGA TGCTTTTGCA CGAGAGCAAA GGGAGAAAAG ACGGCGGAGA
    CTCTTAATGG ATCAGTTAAT AGCTCATGAA GCCCAAGAGG AGGCTTATCG GGAGGAGCAG
    TTGATCAACC GGCTGATGCG ACAGTCCCAG CAGGAACGCA GGATCGCTGT GCAGCTCATG
    CACGTTAGGC ATGAAAAGGA AGTTTTATGG CAAAACAGAA TTTTCAGAGA AAAACAATAT
    GAGGAAAGAC GGCTTAAAGA CTTCCAGGAT GCTCTTGATC GAGAAGCGGC TTTAGCCAGA
    CAAGCCAAGA TTGATTTTGA AGAACAAGCC CTCAGAGATA AGGAAATTCA TGAGCAGATC
    GCCATAGAAA GAGCTCAAGC TCGTTACAAA AAGCATTATT CAATATGTGC AGAAATTTTG
    GATCAGATAC TTGATCTGTC CACTAAGGTG GCAGACTATC GATTGTTGAC AAATAACCTG
    ATCCCATATA AGTTGATGCA TGATTGGAAG GAACTGTTTT TCCATGGAAA ACCCATATAT
    GAGCAAGCCT CTCTTAGACA TTTACCAGCT AAGCTCTCTG CAGAACACCT TGTAGAAATG
    GAGAAAAGGA ACTTGCTAGA TAACAATGAT TATGAAGAAT ATAAGAACAT GGTTGGAGAA
    TGGGCCTTAC CAGAAGAAAT GGTTGAAAAC TTACCACCCT CCAACAATTG CATATTGGGC
    CATGTGATTC ACAGACTAAT TGAAAGTTCT CTTCCTCCTC AAGTTGAATC TACAGTACCT
    GAGTTACCTT CATTTGCTAT AAAAGGATGC TTGTTGGGGA AAACCTTTAG TGGAAAAACT
    ACTGCTCTAA AGGCTCTAGA GAAAAGCTTT CCTATTCAGG TACTTTCTAT TGACACTCTT
    GTTCAAGAAG CTATCCAAGC ATTTCATGAC AATGAAAGTA TCAGCGGGGC CCATGGACTT
    CAGAAAGCAC CTGAAGAAAA GGTTTTACCA GTTAGGCGAA AGAGTGGTAA AGAAAACCAG
    GACGTGCAAG ATGAAGCACT ACCAGAAAGA GAAGATAAAA CCAGACTTGA TACCGATGCT
    AGAAAAGCAG CAAAAGCTGA AGAAGTCGTA GCGAGGGATA GTTTCTCTGA ACTCACTGTG
    CGTGCTCAGC TTGGTGCAAA ATCAGAAATG CTGCTGAAGA AAGGAAAGAG CATATCTGAT
    ATATTGCTAG TCAGCATCAT GGTAAATGCC ATTAATCAGA TACCTATGTA TAAAGGCTGG
    ATCCTGGATG GCTTTCCAAT GACATTAAAC CAAGCAAAAC TTCTGGAGGA AGCACTCACA
    GGCTACAACA GAAACCTCAT AGAACTGGAG GGAAAGAAAT CACAAACATC CACATTAGCT
    GTTGACCCTA CAGCTTCCAA AGAAGTGCCT CTTCCCCCTT CTGCATTTGA TTTTGTCATG
    TTATTAGACA TTTCAGATAA TTCCTCACTG AATCGCATGA ATGACATCAT GGCTAAAGCA
    TTTTCAAGTG AAGCTCCTCG TGAAGATATC AATCAGCGTG TAGCTGCTAA AAACAAAGAT
    ACAGATGAGG ACCAGAATTT AAGAGATCAA ATACAGCACA GAATTGTAGG CTTCCTGGAC
    AACTGGCCTT CGTTGGAGCA GTGGTTTACA GAGCCAGAAA ACATTTTGAT AAAAATCAAT
    GCTGAAGTAG ATAAACAGTC ATTGTTCCAA AAAGTAAAGG AAATGATTAT GACTGAAATA
    ATTAAAAAAG AAAATAAAGT GAAAAAGAAA TTAGAAGAAA AGGAAGCTGA GAGAAAAGAA
    GAAGTTTCCC TGAGTGAGAA GACATCCCCT CCTCCTCCTC CCCCTCCGGA ACCAGAGAAA
    GAGAAGGAAG TCCACCTTCC ACAGGAGCGG TCCAAAACTC CTGCTGCAAA AGGAAAGCAA
    GCATCAGAAC CCCTGCATGG TAATCGAGAA CCTCTTCAGG AAGGAAAAGG GAAGAAAAGT
    GAAACTTCAC TCAAAAGAAA AGGTTCTCCT AGAGGAAAAT CGCTAGGAGG GAAGATTCCA
    CTCAAGAAAT CACCTGCCGA GTCTACAGAT ATTTCACCGG TCCCGGTAGT GCCAGCCCCA
    TCGAAGCCCG GATCAGAAGA GTGGGTGTAT GTAAATGAAC CAATTCCTGA GGAAATACCA
    TCATTTTTAG TACCATACTG GAAATTGATA GAAAACTCCT ATATAAATTC CATCAAAACA
    GTACTCAGGG AGTTGAGAGA AGGCCAGCAT AGTGTACTTG CTTACTTATA TGATATTAGA
    ACAGGCTTCC AGCAGTTTCT GAAGCGTCCA GACCACAAGC AAGACTTCGT GTCTCAGTGG
    CAGGCGGATT TCAACTCCCT TCCTGATGAC CTGTGGGATG ATGAGGAAAC AAAGGCTGAG
    CTGCACCAAA GAGTGAATGA TTTACGGGAC CGCCTGTGGG ACATTTGCGA TGCCCGTAAG
    GAAGAAGCAG AGCAGGAGCG TCTGGACATC ATTAACGAGA GCTGGTTGCA GGACTCAATG
    GGAATTGCCA TGAACCATTT CTTCTCACTG ATGCAGGCAG AACTGAACCG TTTCCAAGAT
    ACGAAGAGAT TCCTGCAAGA TTATTACAGG GCCATGGAAT GTAAAATTCC GATGGATGAC
    ACTAAGAAAT TCACTCGAGT CCCACTGGTC CGACTGGATA GTAGAGATAT TTTGGAAAGC
    CAGCTTAGAA TTCCCCTGGT TCCTCGAAGA TCCATTTCTC CAGAGTCAGC TTTATCCAAA
    CCAAAAACAA AAAGCATCCT GAAGGGCAAG ATCGATTACA CGCTGGAAAG TGTAGAGTTA
    AACTTTGAGA CTGATGAGAA GATGGTGATG GATACGTGGC AGCAGTCTTC TTTGGCAGTA
    TCTCACATGG TGGCTGCTGA AATTCATCAG AGGCTCATGG AAGAAGAAAA AGAAAACCAG
    CCAGCAGAAA CAAAAGAAAA ACTTCCTCAG GCAGCTGCAA ATAAAAAAGT GAAAAAGGAG
    CCTCCGCCAC CGCCACCACC ACCCAAGAAA AAGAAAGAGG ACAAAAAAGG AAAAGGTAAA
    TCCCCACCTA TGGCAGAAGC TACTCCTGTA GTAGTGCCAG CAGAAGAGAC TGAGGAAGTG
    GAATTAAAAA ATGAACTGAA GCTCAGAATA AAGGAAGAGC ATCTTGCTGC CCTGCAATTT
    GAAGAGATAG CTACACAGTT CCGACTTGAA CTGATAAAGG TAAAAGCATT GGCTTTCCTG
    GAAGAGTTAG TAATAAAGGC GGTTGATGTA TATAGACTCA TGGACAAGTG GCTCGGTGAG
    AGGTACTTGA ATGAAATGGC CAGTGTGGAA AAACTAACTG GGGTAGCTCG CTATCACATT
    GAAACATCAA CCAAAATTCA GAATGAACTT TATTTAGACC AAGAAGACTT CTTCATTAAT
    GGCGATATCA AAGTCTTTCC AGATCCACCT CCTCCGGTAC GCCCTCCGCC TGTGGAAAAG
    GAGGAAAATG GCACCCTGAC CATGGAGCAG CTTGACAACC TCCGGGATCA GTTCTTAGAT
    ATAGCGCCCA AAGGCAGGGA TCCTCCAGAC AATGTGAGTG GATTCACCAA GAAGCAGAAA
    AGGCCTCAGA AGCTTGACCA TTACCTCCCA AAGAGGAACA ATCAATTTTT GAAAATTTGG
    CTTAAAAAAT ACCCTTGGCT TGTATACGAT GAGATACTAA ATCTTATGTT CTGCGGCCTG
    TGTCGTAAAC ATGGAGTGAC ATCAAAGGGA AATCAGGTCA GTTTTTTTTA CGGCACGGAT
    AACTTCAGGA CTGAATTTCT CAACGCGCAT CATCTGAGTG AGGCTCACGC CAAGGCGTCC
    CTCATGGAAG CCACAAGTGG TTCTCCAGGG AGCAGAGCTA CCACGGAGCT GATGGTGAGG
    ACCATGAGCA AGGTGACCCT TGGCAGGGTG GAGAACCTGT TCCGGTCCTG CCACGCCATC
    GCCAAGACCG GACGTCCCCT CAAAGACTTC CTCTGGATGT GTTCGCTGGA CGATATGAAG
    GGAATTGATA TCGGCCCGGT GTTCAGAACC AGTAAATCGG CCAGAACTTT TACCTACTTT
    ATTGCTGAAG TGGAACGGAG AACTCTAAGA GAGAAGTTAG AAAAAAGTCA GTTCTTCTCC
    GTCCTCAGTG ATGGGATTGC AGACAATTCA GCTGAGGAAG CTGAACTCGT ATATGTGCAG
    TTTGCCCGCG CAGGGAGAGT GCACTGCCAA ATCGTCGGGG TACAGACCGT GGAGAAGAAG
    GAGCCCTGGG CGATAAAACG TGCCATTGAG AAAACTCTGG AGAGAAATCT TCAACTTCGA
    CTGTCGAACC AAGACTGGGC AAAGAAACTG GTGGGTTTCG GAAGCGATGG AGCTCCAGGT
    TCAGAGGGAG AAAACGGCAG CACAGTGGCC CTGCTTTTAA GGGAAATCCA GCCAGGTGTG
    CTGCCTGTTT ACTGCCTTGC CCATCACCTC GAACTGTCTT ACCAGGCGGT GTTCCAGAGC
    GTTCCCCTCT ACAGCCGTGT GAAAGACCTT CTTCATAGCA TGCACCGCTT CTATCACGAT
    TCTCCTGCTC ACAGGAGTGC TCTGGTGACT GCTTTCAAGG GCCTCCACCT TCAACCTGCG
    ATGCCCTCTC AAGTCAGAGG CCGGAGGTGG CTTCAGGGAT TGCAGGCTGC CCTCCAGAAC
    TTTCTCAAGG GATATCCAGC AATTGTCCAG CAGCTGCAGT CAACAAAAGA AAGCAGAAGC
    AACAGCAGTC ATCAGAAAGC CGAAGAACTC CTGGAGTCTC TCCTCCAGGC AGACATTGTC
    AAATTTGTCC ACTTCTTGGT GGATGTCGTC AATGTCCTTA GCATACTGTC CCATGTCAAT
    CAGAACAGAA ATTCCTCAAT TGCTGATGTA TTTGCCACCT TAGAGTCAAC ACTGGAAATG
    CTCCAAATTT ATCAGACAAG ACCAGGGCCG AAAGAACGTG GGGTAGATTC GGTCACACAC
    TTTCATGGTA ACTGCCTTGG AGGAAAGGGA AACATTTCTG ACGTGAGAAA CATTGTTTTA
    ACACATCTTA TCAAGAGGCT GCGAGGCTGT TTCAGAGATG CCAACCAAGA TGTGGTGAAG
    GCAACCACGA TTGGAAGCTT TAAACTGTGG CCGGCAAAAA TAAATCAAGA ATTTGGAGAA
    AAAGAGGTGG CCATCCTGAT TGCACATTAT GAACCAGTAT TAGAGGCAGC CAAGGTGAAA
    ATTGACCAAG TAGACACAGA ATGGAGCATG TTGAAATTGG AGATCTACTC TAGATTTCAG
    AACATTTGCA AACTGACCTG GGATTTTGTG AATTCTGTTT ATTTACACAA GTATCCAAAT
    ATCCTGACAC TCATTGACCT CGTGCTGAGC CTGCCTGCAA GTTCAGCTGA GGCAGGACGT
    GGCTTTAGTC AGATGAAGCG CATCAGGTCC CAAATGCATG CCAAGGTCAA GGCTGACAAC
    ATGACGGATC TCCTGATAAT TCAGTTGAAT TCTCCAGACA TTAACAACTT TGACCCTAGG
    AAAGCCATTC ATTTATGGAA TACAAGAACA CCATCCTCAA CTGAGGACAC AAGACCTAAC
    TCAGACTGCT CATCAAACTC AGAAAGCCTT AGTGAAAGCG ATGAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OSm32428
    Clone ID Related Accession (Same CDS sequence) XM_015101324.1 , XM_012097082.2
    Accession Version XM_015101324.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5289bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-16
    Organism Ovis aries(sheep)
    Product sperm flagellar protein 2 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014639025.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGTCGGAAA TCCTGTGCCA GTGGCTCAAC CAGGAATTGA AGGTGTCCCA GACCGTGAGT 
    CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAA TTGGAGAAGT TTTGCACAAG
    TTTGCACTTC AGGATGATTT TGCAGACTTT TCAGAAAGCA GGATTTCCAG TGCCAGACTT
    AACAATTTTT CTCGCTTGGA GCCCACACTT CACCTTTTGG GTGTGCAATT TGATCAGAAT
    GTGGCCCACA ATATCATCAC AGAGAAGCCT GGGGCTGCCA CGAAACTCTT ATATCAGTTG
    TACATTGCTC TCCAGAGGAA AAAGAAAGGT GTTCAGACTA GAGTGGAAAT GCCAACTGTA
    CAGCCCCTGA TGAGCACAAA AGTTCAAAAC ATGAAAAGCG AGGCTTTCCG AGATCAACGC
    TTGAGCAAAA GACGACAAAA TGAAATCATG GCCAAAATCC AAGCAGCTAT CATACAGATT
    CCTAAACCCA CATCAAATCG TACTCTGAAA ACATTTGACG CCCAAAAATT AATGAAAAAG
    AAAAAAGAGG CAGAGGATGT GGCCAATGAA ATTAAGAAGT TTGAAGCATT AATAAAAAAG
    GATCTCCAGG CAAAAGAAAG TGCATCCAAG ACTTCTCTAG ATACAGCAGG CCAGACAACC
    GCTGATTTGT TAAACACTTA TTCAGATGAT GACTACATTA AAAAAATCCA AAAACGCCTT
    GAAGAAGATG CTTTTGCACG AGAGCAAAGG GAGAAAAGAC GGCGGAGACT CTTAATGGAT
    CAGTTAATAG CTCATGAAGC CCAAGAGGAG GCTTATCGGG AGGAGCAGTT GATCAACCGG
    CTGATGCGAC AGTCCCAGCA GGAACGCAGG ATCGCTGTGC AGCTCATGCA CGTTAGGCAT
    GAAAAGGAAG TTTTATGGCA AAACAGAATT TTCAGAGAAA AACAATATGA GGAAAGACGG
    CTTAAAGACT TCCAGGATGC TCTTGATCGA GAAGCGGCTT TAGCCAGACA AGCCAAGATT
    GATTTTGAAG AACAAGCCCT CAGAGATAAG GAAATTCATG AGCAGATCGC CATAGAAAGA
    GCTCAAGCTC GTTACAAAAA GCATTATTCA ATATGTGCAG AAATTTTGGA TCAGATACTT
    GATCTGTCCA CTAAGGTGGC AGACTATCGA TTGTTGACAA ATAACCTGAT CCCATATAAG
    TTGATGCATG ATTGGAAGGA ACTGTTTTTC CATGGAAAAC CCATATATGA GCAAGCCTCT
    CTTAGACATT TACCAGCTAA GCTCTCTGCA GAACACCTTG TAGAAATGGA GAAAAGGAAC
    TTGCTAGATA ACAATGATTA TGAAGAATAT AAGAACATGG TTGGAGAATG GGCCTTACCA
    GAAGAAATGG TTGAAAACTT ACCACCCTCC AACAATTGCA TATTGGGCCA TGTGATTCAC
    AGACTAATTG AAAGTTCTCT TCCTCCTCAA GTTGAATCTA CAGTACCTGA GTTACCTTCA
    TTTGCTATAA AAGGATGCTT GTTGGGGAAA ACCTTTAGTG GAAAAACTAC TGCTCTAAAG
    GCTCTAGAGA AAAGCTTTCC TATTCAGGTA CTTTCTATTG ACACTCTTGT TCAAGAAGCT
    ATCCAAGCAT TTCATGACAA TGAAAGTATC AGCGGGGCCC ATGGACTTCA GAAAGCACCT
    GAAGAAAAGG TTTTACCAGT TAGGCGAAAG AGTGGTAAAG AAAACCAGGA CGTGCAAGAT
    GAAGCACTAC CAGAAAGAGA AGATAAAACC AGACTTGATA CCGATGCTAG AAAAGCAGCA
    AAAGCTGAAG AAGTCGTAGC GAGGGATAGT TTCTCTGAAC TCACTGTGCG TGCTCAGCTT
    GGTGCAAAAT CAGAAATGCT GCTGAAGAAA GGAAAGAGCA TATCTGATAT ATTGCTAGTC
    AGCATCATGG TAAATGCCAT TAATCAGATA CCTATGTATA AAGGCTGGAT CCTGGATGGC
    TTTCCAATGA CATTAAACCA AGCAAAACTT CTGGAGGAAG CACTCACAGG CTACAACAGA
    AACCTCATAG AACTGGAGGG AAAGAAATCA CAAACATCCA CATTAGCTGT TGACCCTACA
    GCTTCCAAAG AAGTGCCTCT TCCCCCTTCT GCATTTGATT TTGTCATGTT ATTAGACATT
    TCAGATAATT CCTCACTGAA TCGCATGAAT GACATCATGG CTAAAGCATT TTCAAGTGAA
    GCTCCTCGTG AAGATATCAA TCAGCGTGTA GCTGCTAAAA ACAAAGATAC AGATGAGGAC
    CAGAATTTAA GAGATCAAAT ACAGCACAGA ATTGTAGGCT TCCTGGACAA CTGGCCTTCG
    TTGGAGCAGT GGTTTACAGA GCCAGAAAAC ATTTTGATAA AAATCAATGC TGAAGTAGAT
    AAACAGTCAT TGTTCCAAAA AGTAAAGGAA ATGATTATGA CTGAAATAAT TAAAAAAGAA
    AATAAAGTGA AAAAGAAATT AGAAGAAAAG GAAGCTGAGA GAAAAGAAGA AGTTTCCCTG
    AGTGAGAAGA CATCCCCTCC TCCTCCTCCC CCTCCGGAAC CAGAGAAAGA GAAGGAAGTC
    CACCTTCCAC AGGAGCGGTC CAAAACTCCT GCTGCAAAAG GAAAGCAAGC ATCAGAACCC
    CTGCATGGTA ATCGAGAACC TCTTCAGGAA GGAAAAGGGA AGAAAAGTGA AACTTCACTC
    AAAAGAAAAG GTTCTCCTAG AGGAAAATCG CTAGGAGGGA AGATTCCACT CAAGAAATCA
    CCTGCCGAGT CTACAGATAT TTCACCGGTC CCGGTAGTGC CAGCCCCATC GAAGCCCGGA
    TCAGAAGAGT GGGTGTATGT AAATGAACCA ATTCCTGAGG AAATACCATC ATTTTTAGTA
    CCATACTGGA AATTGATAGA AAACTCCTAT ATAAATTCCA TCAAAACAGT ACTCAGGGAG
    TTGAGAGAAG GCCAGCATAG TGTACTTGCT TACTTATATG ATATTAGAAC AGGCTTCCAG
    CAGTTTCTGA AGCGTCCAGA CCACAAGCAA GACTTCGTGT CTCAGTGGCA GGCGGATTTC
    AACTCCCTTC CTGATGACCT GTGGGATGAT GAGGAAACAA AGGCTGAGCT GCACCAAAGA
    GTGAATGATT TACGGGACCG CCTGTGGGAC ATTTGCGATG CCCGTAAGGA AGAAGCAGAG
    CAGGAGCGTC TGGACATCAT TAACGAGAGC TGGTTGCAGG ACTCAATGGG AATTGCCATG
    AACCATTTCT TCTCACTGAT GCAGGCAGAA CTGAACCGTT TCCAAGATAC GAAGAGATTC
    CTGCAAGATT ATTACAGGGC CATGGAATGT AAAATTCCGA TGGATGACAC TAAGAAATTC
    ACTCGAGTCC CACTGGTCCG ACTGGATAGT AGAGATATTT TGGAAAGCCA GCTTAGAATT
    CCCCTGGTTC CTCGAAGATC CATTTCTCCA GAGTCAGCTT TATCCAAACC AAAAACAAAA
    AGCATCCTGA AGGGCAAGAT CGATTACACG CTGGAAAGTG TAGAGTTAAA CTTTGAGACT
    GATGAGAAGA TGGTGATGGA TACGTGGCAG CAGTCTTCTT TGGCAGTATC TCACATGGTG
    GCTGCTGAAA TTCATCAGAG GCTCATGGAA GAAGAAAAAG AAAACCAGCC AGCAGAAACA
    AAAGAAAAAC TTCCTCAGGC AGCTGCAAAT AAAAAAGTGA AAAAGGAGCC TCCGCCACCG
    CCACCACCAC CCAAGAAAAA GAAAGAGGAC AAAAAAGGAA AAGGTAAATC CCCACCTATG
    GCAGAAGCTA CTCCTGTAGT AGTGCCAGCA GAAGAGACTG AGGAAGTGGA ATTAAAAAAT
    GAACTGAAGC TCAGAATAAA GGAAGAGCAT CTTGCTGCCC TGCAATTTGA AGAGATAGCT
    ACACAGTTCC GACTTGAACT GATAAAGGTA AAAGCATTGG CTTTCCTGGA AGAGTTAGTA
    ATAAAGGCGG TTGATGTATA TAGACTCATG GACAAGTGGC TCGGTGAGAG GTACTTGAAT
    GAAATGGCCA GTGTGGAAAA ACTAACTGGG GTAGCTCGCT ATCACATTGA AACATCAACC
    AAAATTCAGA ATGAACTTTA TTTAGACCAA GAAGACTTCT TCATTAATGG CGATATCAAA
    GTCTTTCCAG ATCCACCTCC TCCGGTACGC CCTCCGCCTG TGGAAAAGGA GGAAAATGGC
    ACCCTGACCA TGGAGCAGCT TGACAACCTC CGGGATCAGT TCTTAGATAT AGCGCCCAAA
    GGTATAATAG GAAATAAAGC ATTTGCTGAC ATTCTGCTGG ATTTGGTAAC TCTGAACCTT
    GGAACAAACA ACTTTCCTAC TAGTTGGATG AACCTCACTC AACCGGAATT GCAGGAGTTA
    GCATCTTTGT TGGTAACAAA CTCGGAGTCC GTGGACTGGC GGAAATTCTT GTTAGTAGTA
    GCGCTGCCTT GGCCCATCCC CCTGGAGGAG GAGCTTCTGG AGACCCTGCA GAGGTTCAAG
    GCCCTGGATG CGGAGCAGCT GGGCACCGTC ACTTATGAAC AGTATAAGCA GGCTGGCCTG
    TGGTTTACAG GAGATGAAGA TATCAAAATT CCAGAAAATC CTCTTGAACC CCTGCCATTC
    AATAGGCAGG AGCATCTTAT AGAGTTTTTC TTTAGGTTGT TTGCTGACTG CGAGAAGGTT
    CCACCCCAGC TTGACTACAC ACAGATGCTG CTTTATTTTG CTTGCCATCC AGACAGCACA
    GAAGGGGTCT ACAGGGCCCT CAGCGTAGCC ATTGGGACTC ACGTCTTCCA GCCAATTGAA
    ATGCCTCATC CTGTTGCAGC AAAGACCTCT TCCTTTACTG ATATGAGTCC CATTGAGGAA
    TATCCTGAAC TCGAGGACAA CTTTATGAGT GAGGAAAGGG AATTACAAGA GGACGGGGAG
    GAAAAGGAAG AAGAAATCCC TGAAAATGCA AATACGGAAA TGATTTCCAT GCAAACATTA
    CTCCAAGTGT TCCGAGGAGG AAATGAAGTA CTGGACGCTA ACAGATTTGC CAGCCACCAA
    AAGATAGAGA ACATTTATTT AGAGAACTTC ATAAAAGTGT TTCAGGACCT AGGTTCCAAG
    AACCTTGAGC CAATTGAAGT TGCTGTTCTT CTGAAGCATC CTTTTATTCA AGACCTGATT
    TCAAGTTATC CAGACTATAA GATCCCTGGT GCTTCTGCCG AGTCGGCTTC TTCAACCCTC
    CCCCGTCCTG GTCCTTCATC TCTCCTCCCT GTCATCACTT CACCCTGGAA TCTGACAAGA
    GGACAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_014956810.1
    CDS493..5781
    Translation

    Target ORF information:

    RefSeq Version XM_015101324.1
    Organism Ovis aries(sheep)
    Definition Ovis aries sperm flagellar 2 (SPEF2), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015101324.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGTCGGAAA TCCTGTGCCA GTGGCTCAAC CAGGAATTGA AGGTGTCCCA GACCGTGAGT 
    CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAA TTGGAGAAGT TTTGCACAAG
    TTTGCACTTC AGGATGATTT TGCAGACTTT TCAGAAAGCA GGATTTCCAG TGCCAGACTT
    AACAATTTTT CTCGCTTGGA GCCCACACTT CACCTTTTGG GTGTGCAATT TGATCAGAAT
    GTGGCCCACA ATATCATCAC AGAGAAGCCT GGGGCTGCCA CGAAACTCTT ATATCAGTTG
    TACATTGCTC TCCAGAGGAA AAAGAAAGGT GTTCAGACTA GAGTGGAAAT GCCAACTGTA
    CAGCCCCTGA TGAGCACAAA AGTTCAAAAC ATGAAAAGCG AGGCTTTCCG AGATCAACGC
    TTGAGCAAAA GACGACAAAA TGAAATCATG GCCAAAATCC AAGCAGCTAT CATACAGATT
    CCTAAACCCA CATCAAATCG TACTCTGAAA ACATTTGACG CCCAAAAATT AATGAAAAAG
    AAAAAAGAGG CAGAGGATGT GGCCAATGAA ATTAAGAAGT TTGAAGCATT AATAAAAAAG
    GATCTCCAGG CAAAAGAAAG TGCATCCAAG ACTTCTCTAG ATACAGCAGG CCAGACAACC
    GCTGATTTGT TAAACACTTA TTCAGATGAT GACTACATTA AAAAAATCCA AAAACGCCTT
    GAAGAAGATG CTTTTGCACG AGAGCAAAGG GAGAAAAGAC GGCGGAGACT CTTAATGGAT
    CAGTTAATAG CTCATGAAGC CCAAGAGGAG GCTTATCGGG AGGAGCAGTT GATCAACCGG
    CTGATGCGAC AGTCCCAGCA GGAACGCAGG ATCGCTGTGC AGCTCATGCA CGTTAGGCAT
    GAAAAGGAAG TTTTATGGCA AAACAGAATT TTCAGAGAAA AACAATATGA GGAAAGACGG
    CTTAAAGACT TCCAGGATGC TCTTGATCGA GAAGCGGCTT TAGCCAGACA AGCCAAGATT
    GATTTTGAAG AACAAGCCCT CAGAGATAAG GAAATTCATG AGCAGATCGC CATAGAAAGA
    GCTCAAGCTC GTTACAAAAA GCATTATTCA ATATGTGCAG AAATTTTGGA TCAGATACTT
    GATCTGTCCA CTAAGGTGGC AGACTATCGA TTGTTGACAA ATAACCTGAT CCCATATAAG
    TTGATGCATG ATTGGAAGGA ACTGTTTTTC CATGGAAAAC CCATATATGA GCAAGCCTCT
    CTTAGACATT TACCAGCTAA GCTCTCTGCA GAACACCTTG TAGAAATGGA GAAAAGGAAC
    TTGCTAGATA ACAATGATTA TGAAGAATAT AAGAACATGG TTGGAGAATG GGCCTTACCA
    GAAGAAATGG TTGAAAACTT ACCACCCTCC AACAATTGCA TATTGGGCCA TGTGATTCAC
    AGACTAATTG AAAGTTCTCT TCCTCCTCAA GTTGAATCTA CAGTACCTGA GTTACCTTCA
    TTTGCTATAA AAGGATGCTT GTTGGGGAAA ACCTTTAGTG GAAAAACTAC TGCTCTAAAG
    GCTCTAGAGA AAAGCTTTCC TATTCAGGTA CTTTCTATTG ACACTCTTGT TCAAGAAGCT
    ATCCAAGCAT TTCATGACAA TGAAAGTATC AGCGGGGCCC ATGGACTTCA GAAAGCACCT
    GAAGAAAAGG TTTTACCAGT TAGGCGAAAG AGTGGTAAAG AAAACCAGGA CGTGCAAGAT
    GAAGCACTAC CAGAAAGAGA AGATAAAACC AGACTTGATA CCGATGCTAG AAAAGCAGCA
    AAAGCTGAAG AAGTCGTAGC GAGGGATAGT TTCTCTGAAC TCACTGTGCG TGCTCAGCTT
    GGTGCAAAAT CAGAAATGCT GCTGAAGAAA GGAAAGAGCA TATCTGATAT ATTGCTAGTC
    AGCATCATGG TAAATGCCAT TAATCAGATA CCTATGTATA AAGGCTGGAT CCTGGATGGC
    TTTCCAATGA CATTAAACCA AGCAAAACTT CTGGAGGAAG CACTCACAGG CTACAACAGA
    AACCTCATAG AACTGGAGGG AAAGAAATCA CAAACATCCA CATTAGCTGT TGACCCTACA
    GCTTCCAAAG AAGTGCCTCT TCCCCCTTCT GCATTTGATT TTGTCATGTT ATTAGACATT
    TCAGATAATT CCTCACTGAA TCGCATGAAT GACATCATGG CTAAAGCATT TTCAAGTGAA
    GCTCCTCGTG AAGATATCAA TCAGCGTGTA GCTGCTAAAA ACAAAGATAC AGATGAGGAC
    CAGAATTTAA GAGATCAAAT ACAGCACAGA ATTGTAGGCT TCCTGGACAA CTGGCCTTCG
    TTGGAGCAGT GGTTTACAGA GCCAGAAAAC ATTTTGATAA AAATCAATGC TGAAGTAGAT
    AAACAGTCAT TGTTCCAAAA AGTAAAGGAA ATGATTATGA CTGAAATAAT TAAAAAAGAA
    AATAAAGTGA AAAAGAAATT AGAAGAAAAG GAAGCTGAGA GAAAAGAAGA AGTTTCCCTG
    AGTGAGAAGA CATCCCCTCC TCCTCCTCCC CCTCCGGAAC CAGAGAAAGA GAAGGAAGTC
    CACCTTCCAC AGGAGCGGTC CAAAACTCCT GCTGCAAAAG GAAAGCAAGC ATCAGAACCC
    CTGCATGGTA ATCGAGAACC TCTTCAGGAA GGAAAAGGGA AGAAAAGTGA AACTTCACTC
    AAAAGAAAAG GTTCTCCTAG AGGAAAATCG CTAGGAGGGA AGATTCCACT CAAGAAATCA
    CCTGCCGAGT CTACAGATAT TTCACCGGTC CCGGTAGTGC CAGCCCCATC GAAGCCCGGA
    TCAGAAGAGT GGGTGTATGT AAATGAACCA ATTCCTGAGG AAATACCATC ATTTTTAGTA
    CCATACTGGA AATTGATAGA AAACTCCTAT ATAAATTCCA TCAAAACAGT ACTCAGGGAG
    TTGAGAGAAG GCCAGCATAG TGTACTTGCT TACTTATATG ATATTAGAAC AGGCTTCCAG
    CAGTTTCTGA AGCGTCCAGA CCACAAGCAA GACTTCGTGT CTCAGTGGCA GGCGGATTTC
    AACTCCCTTC CTGATGACCT GTGGGATGAT GAGGAAACAA AGGCTGAGCT GCACCAAAGA
    GTGAATGATT TACGGGACCG CCTGTGGGAC ATTTGCGATG CCCGTAAGGA AGAAGCAGAG
    CAGGAGCGTC TGGACATCAT TAACGAGAGC TGGTTGCAGG ACTCAATGGG AATTGCCATG
    AACCATTTCT TCTCACTGAT GCAGGCAGAA CTGAACCGTT TCCAAGATAC GAAGAGATTC
    CTGCAAGATT ATTACAGGGC CATGGAATGT AAAATTCCGA TGGATGACAC TAAGAAATTC
    ACTCGAGTCC CACTGGTCCG ACTGGATAGT AGAGATATTT TGGAAAGCCA GCTTAGAATT
    CCCCTGGTTC CTCGAAGATC CATTTCTCCA GAGTCAGCTT TATCCAAACC AAAAACAAAA
    AGCATCCTGA AGGGCAAGAT CGATTACACG CTGGAAAGTG TAGAGTTAAA CTTTGAGACT
    GATGAGAAGA TGGTGATGGA TACGTGGCAG CAGTCTTCTT TGGCAGTATC TCACATGGTG
    GCTGCTGAAA TTCATCAGAG GCTCATGGAA GAAGAAAAAG AAAACCAGCC AGCAGAAACA
    AAAGAAAAAC TTCCTCAGGC AGCTGCAAAT AAAAAAGTGA AAAAGGAGCC TCCGCCACCG
    CCACCACCAC CCAAGAAAAA GAAAGAGGAC AAAAAAGGAA AAGGTAAATC CCCACCTATG
    GCAGAAGCTA CTCCTGTAGT AGTGCCAGCA GAAGAGACTG AGGAAGTGGA ATTAAAAAAT
    GAACTGAAGC TCAGAATAAA GGAAGAGCAT CTTGCTGCCC TGCAATTTGA AGAGATAGCT
    ACACAGTTCC GACTTGAACT GATAAAGGTA AAAGCATTGG CTTTCCTGGA AGAGTTAGTA
    ATAAAGGCGG TTGATGTATA TAGACTCATG GACAAGTGGC TCGGTGAGAG GTACTTGAAT
    GAAATGGCCA GTGTGGAAAA ACTAACTGGG GTAGCTCGCT ATCACATTGA AACATCAACC
    AAAATTCAGA ATGAACTTTA TTTAGACCAA GAAGACTTCT TCATTAATGG CGATATCAAA
    GTCTTTCCAG ATCCACCTCC TCCGGTACGC CCTCCGCCTG TGGAAAAGGA GGAAAATGGC
    ACCCTGACCA TGGAGCAGCT TGACAACCTC CGGGATCAGT TCTTAGATAT AGCGCCCAAA
    GGTATAATAG GAAATAAAGC ATTTGCTGAC ATTCTGCTGG ATTTGGTAAC TCTGAACCTT
    GGAACAAACA ACTTTCCTAC TAGTTGGATG AACCTCACTC AACCGGAATT GCAGGAGTTA
    GCATCTTTGT TGGTAACAAA CTCGGAGTCC GTGGACTGGC GGAAATTCTT GTTAGTAGTA
    GCGCTGCCTT GGCCCATCCC CCTGGAGGAG GAGCTTCTGG AGACCCTGCA GAGGTTCAAG
    GCCCTGGATG CGGAGCAGCT GGGCACCGTC ACTTATGAAC AGTATAAGCA GGCTGGCCTG
    TGGTTTACAG GAGATGAAGA TATCAAAATT CCAGAAAATC CTCTTGAACC CCTGCCATTC
    AATAGGCAGG AGCATCTTAT AGAGTTTTTC TTTAGGTTGT TTGCTGACTG CGAGAAGGTT
    CCACCCCAGC TTGACTACAC ACAGATGCTG CTTTATTTTG CTTGCCATCC AGACAGCACA
    GAAGGGGTCT ACAGGGCCCT CAGCGTAGCC ATTGGGACTC ACGTCTTCCA GCCAATTGAA
    ATGCCTCATC CTGTTGCAGC AAAGACCTCT TCCTTTACTG ATATGAGTCC CATTGAGGAA
    TATCCTGAAC TCGAGGACAA CTTTATGAGT GAGGAAAGGG AATTACAAGA GGACGGGGAG
    GAAAAGGAAG AAGAAATCCC TGAAAATGCA AATACGGAAA TGATTTCCAT GCAAACATTA
    CTCCAAGTGT TCCGAGGAGG AAATGAAGTA CTGGACGCTA ACAGATTTGC CAGCCACCAA
    AAGATAGAGA ACATTTATTT AGAGAACTTC ATAAAAGTGT TTCAGGACCT AGGTTCCAAG
    AACCTTGAGC CAATTGAAGT TGCTGTTCTT CTGAAGCATC CTTTTATTCA AGACCTGATT
    TCAAGTTATC CAGACTATAA GATCCCTGGT GCTTCTGCCG AGTCGGCTTC TTCAACCCTC
    CCCCGTCCTG GTCCTTCATC TCTCCTCCCT GTCATCACTT CACCCTGGAA TCTGACAAGA
    GGACAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OSm32428
    Clone ID Related Accession (Same CDS sequence) XM_015101324.1 , XM_012097082.2
    Accession Version XM_012097082.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5289bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-16
    Organism Ovis aries(sheep)
    Product sperm flagellar protein 2 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014639025.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012097082.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGTCGGAAA TCCTGTGCCA GTGGCTCAAC CAGGAATTGA AGGTGTCCCA GACCGTGAGT 
    CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAA TTGGAGAAGT TTTGCACAAG
    TTTGCACTTC AGGATGATTT TGCAGACTTT TCAGAAAGCA GGATTTCCAG TGCCAGACTT
    AACAATTTTT CTCGCTTGGA GCCCACACTT CACCTTTTGG GTGTGCAATT TGATCAGAAT
    GTGGCCCACA ATATCATCAC AGAGAAGCCT GGGGCTGCCA CGAAACTCTT ATATCAGTTG
    TACATTGCTC TCCAGAGGAA AAAGAAAGGT GTTCAGACTA GAGTGGAAAT GCCAACTGTA
    CAGCCCCTGA TGAGCACAAA AGTTCAAAAC ATGAAAAGCG AGGCTTTCCG AGATCAACGC
    TTGAGCAAAA GACGACAAAA TGAAATCATG GCCAAAATCC AAGCAGCTAT CATACAGATT
    CCTAAACCCA CATCAAATCG TACTCTGAAA ACATTTGACG CCCAAAAATT AATGAAAAAG
    AAAAAAGAGG CAGAGGATGT GGCCAATGAA ATTAAGAAGT TTGAAGCATT AATAAAAAAG
    GATCTCCAGG CAAAAGAAAG TGCATCCAAG ACTTCTCTAG ATACAGCAGG CCAGACAACC
    GCTGATTTGT TAAACACTTA TTCAGATGAT GACTACATTA AAAAAATCCA AAAACGCCTT
    GAAGAAGATG CTTTTGCACG AGAGCAAAGG GAGAAAAGAC GGCGGAGACT CTTAATGGAT
    CAGTTAATAG CTCATGAAGC CCAAGAGGAG GCTTATCGGG AGGAGCAGTT GATCAACCGG
    CTGATGCGAC AGTCCCAGCA GGAACGCAGG ATCGCTGTGC AGCTCATGCA CGTTAGGCAT
    GAAAAGGAAG TTTTATGGCA AAACAGAATT TTCAGAGAAA AACAATATGA GGAAAGACGG
    CTTAAAGACT TCCAGGATGC TCTTGATCGA GAAGCGGCTT TAGCCAGACA AGCCAAGATT
    GATTTTGAAG AACAAGCCCT CAGAGATAAG GAAATTCATG AGCAGATCGC CATAGAAAGA
    GCTCAAGCTC GTTACAAAAA GCATTATTCA ATATGTGCAG AAATTTTGGA TCAGATACTT
    GATCTGTCCA CTAAGGTGGC AGACTATCGA TTGTTGACAA ATAACCTGAT CCCATATAAG
    TTGATGCATG ATTGGAAGGA ACTGTTTTTC CATGGAAAAC CCATATATGA GCAAGCCTCT
    CTTAGACATT TACCAGCTAA GCTCTCTGCA GAACACCTTG TAGAAATGGA GAAAAGGAAC
    TTGCTAGATA ACAATGATTA TGAAGAATAT AAGAACATGG TTGGAGAATG GGCCTTACCA
    GAAGAAATGG TTGAAAACTT ACCACCCTCC AACAATTGCA TATTGGGCCA TGTGATTCAC
    AGACTAATTG AAAGTTCTCT TCCTCCTCAA GTTGAATCTA CAGTACCTGA GTTACCTTCA
    TTTGCTATAA AAGGATGCTT GTTGGGGAAA ACCTTTAGTG GAAAAACTAC TGCTCTAAAG
    GCTCTAGAGA AAAGCTTTCC TATTCAGGTA CTTTCTATTG ACACTCTTGT TCAAGAAGCT
    ATCCAAGCAT TTCATGACAA TGAAAGTATC AGCGGGGCCC ATGGACTTCA GAAAGCACCT
    GAAGAAAAGG TTTTACCAGT TAGGCGAAAG AGTGGTAAAG AAAACCAGGA CGTGCAAGAT
    GAAGCACTAC CAGAAAGAGA AGATAAAACC AGACTTGATA CCGATGCTAG AAAAGCAGCA
    AAAGCTGAAG AAGTCGTAGC GAGGGATAGT TTCTCTGAAC TCACTGTGCG TGCTCAGCTT
    GGTGCAAAAT CAGAAATGCT GCTGAAGAAA GGAAAGAGCA TATCTGATAT ATTGCTAGTC
    AGCATCATGG TAAATGCCAT TAATCAGATA CCTATGTATA AAGGCTGGAT CCTGGATGGC
    TTTCCAATGA CATTAAACCA AGCAAAACTT CTGGAGGAAG CACTCACAGG CTACAACAGA
    AACCTCATAG AACTGGAGGG AAAGAAATCA CAAACATCCA CATTAGCTGT TGACCCTACA
    GCTTCCAAAG AAGTGCCTCT TCCCCCTTCT GCATTTGATT TTGTCATGTT ATTAGACATT
    TCAGATAATT CCTCACTGAA TCGCATGAAT GACATCATGG CTAAAGCATT TTCAAGTGAA
    GCTCCTCGTG AAGATATCAA TCAGCGTGTA GCTGCTAAAA ACAAAGATAC AGATGAGGAC
    CAGAATTTAA GAGATCAAAT ACAGCACAGA ATTGTAGGCT TCCTGGACAA CTGGCCTTCG
    TTGGAGCAGT GGTTTACAGA GCCAGAAAAC ATTTTGATAA AAATCAATGC TGAAGTAGAT
    AAACAGTCAT TGTTCCAAAA AGTAAAGGAA ATGATTATGA CTGAAATAAT TAAAAAAGAA
    AATAAAGTGA AAAAGAAATT AGAAGAAAAG GAAGCTGAGA GAAAAGAAGA AGTTTCCCTG
    AGTGAGAAGA CATCCCCTCC TCCTCCTCCC CCTCCGGAAC CAGAGAAAGA GAAGGAAGTC
    CACCTTCCAC AGGAGCGGTC CAAAACTCCT GCTGCAAAAG GAAAGCAAGC ATCAGAACCC
    CTGCATGGTA ATCGAGAACC TCTTCAGGAA GGAAAAGGGA AGAAAAGTGA AACTTCACTC
    AAAAGAAAAG GTTCTCCTAG AGGAAAATCG CTAGGAGGGA AGATTCCACT CAAGAAATCA
    CCTGCCGAGT CTACAGATAT TTCACCGGTC CCGGTAGTGC CAGCCCCATC GAAGCCCGGA
    TCAGAAGAGT GGGTGTATGT AAATGAACCA ATTCCTGAGG AAATACCATC ATTTTTAGTA
    CCATACTGGA AATTGATAGA AAACTCCTAT ATAAATTCCA TCAAAACAGT ACTCAGGGAG
    TTGAGAGAAG GCCAGCATAG TGTACTTGCT TACTTATATG ATATTAGAAC AGGCTTCCAG
    CAGTTTCTGA AGCGTCCAGA CCACAAGCAA GACTTCGTGT CTCAGTGGCA GGCGGATTTC
    AACTCCCTTC CTGATGACCT GTGGGATGAT GAGGAAACAA AGGCTGAGCT GCACCAAAGA
    GTGAATGATT TACGGGACCG CCTGTGGGAC ATTTGCGATG CCCGTAAGGA AGAAGCAGAG
    CAGGAGCGTC TGGACATCAT TAACGAGAGC TGGTTGCAGG ACTCAATGGG AATTGCCATG
    AACCATTTCT TCTCACTGAT GCAGGCAGAA CTGAACCGTT TCCAAGATAC GAAGAGATTC
    CTGCAAGATT ATTACAGGGC CATGGAATGT AAAATTCCGA TGGATGACAC TAAGAAATTC
    ACTCGAGTCC CACTGGTCCG ACTGGATAGT AGAGATATTT TGGAAAGCCA GCTTAGAATT
    CCCCTGGTTC CTCGAAGATC CATTTCTCCA GAGTCAGCTT TATCCAAACC AAAAACAAAA
    AGCATCCTGA AGGGCAAGAT CGATTACACG CTGGAAAGTG TAGAGTTAAA CTTTGAGACT
    GATGAGAAGA TGGTGATGGA TACGTGGCAG CAGTCTTCTT TGGCAGTATC TCACATGGTG
    GCTGCTGAAA TTCATCAGAG GCTCATGGAA GAAGAAAAAG AAAACCAGCC AGCAGAAACA
    AAAGAAAAAC TTCCTCAGGC AGCTGCAAAT AAAAAAGTGA AAAAGGAGCC TCCGCCACCG
    CCACCACCAC CCAAGAAAAA GAAAGAGGAC AAAAAAGGAA AAGGTAAATC CCCACCTATG
    GCAGAAGCTA CTCCTGTAGT AGTGCCAGCA GAAGAGACTG AGGAAGTGGA ATTAAAAAAT
    GAACTGAAGC TCAGAATAAA GGAAGAGCAT CTTGCTGCCC TGCAATTTGA AGAGATAGCT
    ACACAGTTCC GACTTGAACT GATAAAGGTA AAAGCATTGG CTTTCCTGGA AGAGTTAGTA
    ATAAAGGCGG TTGATGTATA TAGACTCATG GACAAGTGGC TCGGTGAGAG GTACTTGAAT
    GAAATGGCCA GTGTGGAAAA ACTAACTGGG GTAGCTCGCT ATCACATTGA AACATCAACC
    AAAATTCAGA ATGAACTTTA TTTAGACCAA GAAGACTTCT TCATTAATGG CGATATCAAA
    GTCTTTCCAG ATCCACCTCC TCCGGTACGC CCTCCGCCTG TGGAAAAGGA GGAAAATGGC
    ACCCTGACCA TGGAGCAGCT TGACAACCTC CGGGATCAGT TCTTAGATAT AGCGCCCAAA
    GGTATAATAG GAAATAAAGC ATTTGCTGAC ATTCTGCTGG ATTTGGTAAC TCTGAACCTT
    GGAACAAACA ACTTTCCTAC TAGTTGGATG AACCTCACTC AACCGGAATT GCAGGAGTTA
    GCATCTTTGT TGGTAACAAA CTCGGAGTCC GTGGACTGGC GGAAATTCTT GTTAGTAGTA
    GCGCTGCCTT GGCCCATCCC CCTGGAGGAG GAGCTTCTGG AGACCCTGCA GAGGTTCAAG
    GCCCTGGATG CGGAGCAGCT GGGCACCGTC ACTTATGAAC AGTATAAGCA GGCTGGCCTG
    TGGTTTACAG GAGATGAAGA TATCAAAATT CCAGAAAATC CTCTTGAACC CCTGCCATTC
    AATAGGCAGG AGCATCTTAT AGAGTTTTTC TTTAGGTTGT TTGCTGACTG CGAGAAGGTT
    CCACCCCAGC TTGACTACAC ACAGATGCTG CTTTATTTTG CTTGCCATCC AGACAGCACA
    GAAGGGGTCT ACAGGGCCCT CAGCGTAGCC ATTGGGACTC ACGTCTTCCA GCCAATTGAA
    ATGCCTCATC CTGTTGCAGC AAAGACCTCT TCCTTTACTG ATATGAGTCC CATTGAGGAA
    TATCCTGAAC TCGAGGACAA CTTTATGAGT GAGGAAAGGG AATTACAAGA GGACGGGGAG
    GAAAAGGAAG AAGAAATCCC TGAAAATGCA AATACGGAAA TGATTTCCAT GCAAACATTA
    CTCCAAGTGT TCCGAGGAGG AAATGAAGTA CTGGACGCTA ACAGATTTGC CAGCCACCAA
    AAGATAGAGA ACATTTATTT AGAGAACTTC ATAAAAGTGT TTCAGGACCT AGGTTCCAAG
    AACCTTGAGC CAATTGAAGT TGCTGTTCTT CTGAAGCATC CTTTTATTCA AGACCTGATT
    TCAAGTTATC CAGACTATAA GATCCCTGGT GCTTCTGCCG AGTCGGCTTC TTCAACCCTC
    CCCCGTCCTG GTCCTTCATC TCTCCTCCCT GTCATCACTT CACCCTGGAA TCTGACAAGA
    GGACAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011952472.1
    CDS493..5781
    Translation

    Target ORF information:

    RefSeq Version XM_012097082.2
    Organism Ovis aries(sheep)
    Definition Ovis aries sperm flagellar 2 (SPEF2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012097082.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGTCGGAAA TCCTGTGCCA GTGGCTCAAC CAGGAATTGA AGGTGTCCCA GACCGTGAGT 
    CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAA TTGGAGAAGT TTTGCACAAG
    TTTGCACTTC AGGATGATTT TGCAGACTTT TCAGAAAGCA GGATTTCCAG TGCCAGACTT
    AACAATTTTT CTCGCTTGGA GCCCACACTT CACCTTTTGG GTGTGCAATT TGATCAGAAT
    GTGGCCCACA ATATCATCAC AGAGAAGCCT GGGGCTGCCA CGAAACTCTT ATATCAGTTG
    TACATTGCTC TCCAGAGGAA AAAGAAAGGT GTTCAGACTA GAGTGGAAAT GCCAACTGTA
    CAGCCCCTGA TGAGCACAAA AGTTCAAAAC ATGAAAAGCG AGGCTTTCCG AGATCAACGC
    TTGAGCAAAA GACGACAAAA TGAAATCATG GCCAAAATCC AAGCAGCTAT CATACAGATT
    CCTAAACCCA CATCAAATCG TACTCTGAAA ACATTTGACG CCCAAAAATT AATGAAAAAG
    AAAAAAGAGG CAGAGGATGT GGCCAATGAA ATTAAGAAGT TTGAAGCATT AATAAAAAAG
    GATCTCCAGG CAAAAGAAAG TGCATCCAAG ACTTCTCTAG ATACAGCAGG CCAGACAACC
    GCTGATTTGT TAAACACTTA TTCAGATGAT GACTACATTA AAAAAATCCA AAAACGCCTT
    GAAGAAGATG CTTTTGCACG AGAGCAAAGG GAGAAAAGAC GGCGGAGACT CTTAATGGAT
    CAGTTAATAG CTCATGAAGC CCAAGAGGAG GCTTATCGGG AGGAGCAGTT GATCAACCGG
    CTGATGCGAC AGTCCCAGCA GGAACGCAGG ATCGCTGTGC AGCTCATGCA CGTTAGGCAT
    GAAAAGGAAG TTTTATGGCA AAACAGAATT TTCAGAGAAA AACAATATGA GGAAAGACGG
    CTTAAAGACT TCCAGGATGC TCTTGATCGA GAAGCGGCTT TAGCCAGACA AGCCAAGATT
    GATTTTGAAG AACAAGCCCT CAGAGATAAG GAAATTCATG AGCAGATCGC CATAGAAAGA
    GCTCAAGCTC GTTACAAAAA GCATTATTCA ATATGTGCAG AAATTTTGGA TCAGATACTT
    GATCTGTCCA CTAAGGTGGC AGACTATCGA TTGTTGACAA ATAACCTGAT CCCATATAAG
    TTGATGCATG ATTGGAAGGA ACTGTTTTTC CATGGAAAAC CCATATATGA GCAAGCCTCT
    CTTAGACATT TACCAGCTAA GCTCTCTGCA GAACACCTTG TAGAAATGGA GAAAAGGAAC
    TTGCTAGATA ACAATGATTA TGAAGAATAT AAGAACATGG TTGGAGAATG GGCCTTACCA
    GAAGAAATGG TTGAAAACTT ACCACCCTCC AACAATTGCA TATTGGGCCA TGTGATTCAC
    AGACTAATTG AAAGTTCTCT TCCTCCTCAA GTTGAATCTA CAGTACCTGA GTTACCTTCA
    TTTGCTATAA AAGGATGCTT GTTGGGGAAA ACCTTTAGTG GAAAAACTAC TGCTCTAAAG
    GCTCTAGAGA AAAGCTTTCC TATTCAGGTA CTTTCTATTG ACACTCTTGT TCAAGAAGCT
    ATCCAAGCAT TTCATGACAA TGAAAGTATC AGCGGGGCCC ATGGACTTCA GAAAGCACCT
    GAAGAAAAGG TTTTACCAGT TAGGCGAAAG AGTGGTAAAG AAAACCAGGA CGTGCAAGAT
    GAAGCACTAC CAGAAAGAGA AGATAAAACC AGACTTGATA CCGATGCTAG AAAAGCAGCA
    AAAGCTGAAG AAGTCGTAGC GAGGGATAGT TTCTCTGAAC TCACTGTGCG TGCTCAGCTT
    GGTGCAAAAT CAGAAATGCT GCTGAAGAAA GGAAAGAGCA TATCTGATAT ATTGCTAGTC
    AGCATCATGG TAAATGCCAT TAATCAGATA CCTATGTATA AAGGCTGGAT CCTGGATGGC
    TTTCCAATGA CATTAAACCA AGCAAAACTT CTGGAGGAAG CACTCACAGG CTACAACAGA
    AACCTCATAG AACTGGAGGG AAAGAAATCA CAAACATCCA CATTAGCTGT TGACCCTACA
    GCTTCCAAAG AAGTGCCTCT TCCCCCTTCT GCATTTGATT TTGTCATGTT ATTAGACATT
    TCAGATAATT CCTCACTGAA TCGCATGAAT GACATCATGG CTAAAGCATT TTCAAGTGAA
    GCTCCTCGTG AAGATATCAA TCAGCGTGTA GCTGCTAAAA ACAAAGATAC AGATGAGGAC
    CAGAATTTAA GAGATCAAAT ACAGCACAGA ATTGTAGGCT TCCTGGACAA CTGGCCTTCG
    TTGGAGCAGT GGTTTACAGA GCCAGAAAAC ATTTTGATAA AAATCAATGC TGAAGTAGAT
    AAACAGTCAT TGTTCCAAAA AGTAAAGGAA ATGATTATGA CTGAAATAAT TAAAAAAGAA
    AATAAAGTGA AAAAGAAATT AGAAGAAAAG GAAGCTGAGA GAAAAGAAGA AGTTTCCCTG
    AGTGAGAAGA CATCCCCTCC TCCTCCTCCC CCTCCGGAAC CAGAGAAAGA GAAGGAAGTC
    CACCTTCCAC AGGAGCGGTC CAAAACTCCT GCTGCAAAAG GAAAGCAAGC ATCAGAACCC
    CTGCATGGTA ATCGAGAACC TCTTCAGGAA GGAAAAGGGA AGAAAAGTGA AACTTCACTC
    AAAAGAAAAG GTTCTCCTAG AGGAAAATCG CTAGGAGGGA AGATTCCACT CAAGAAATCA
    CCTGCCGAGT CTACAGATAT TTCACCGGTC CCGGTAGTGC CAGCCCCATC GAAGCCCGGA
    TCAGAAGAGT GGGTGTATGT AAATGAACCA ATTCCTGAGG AAATACCATC ATTTTTAGTA
    CCATACTGGA AATTGATAGA AAACTCCTAT ATAAATTCCA TCAAAACAGT ACTCAGGGAG
    TTGAGAGAAG GCCAGCATAG TGTACTTGCT TACTTATATG ATATTAGAAC AGGCTTCCAG
    CAGTTTCTGA AGCGTCCAGA CCACAAGCAA GACTTCGTGT CTCAGTGGCA GGCGGATTTC
    AACTCCCTTC CTGATGACCT GTGGGATGAT GAGGAAACAA AGGCTGAGCT GCACCAAAGA
    GTGAATGATT TACGGGACCG CCTGTGGGAC ATTTGCGATG CCCGTAAGGA AGAAGCAGAG
    CAGGAGCGTC TGGACATCAT TAACGAGAGC TGGTTGCAGG ACTCAATGGG AATTGCCATG
    AACCATTTCT TCTCACTGAT GCAGGCAGAA CTGAACCGTT TCCAAGATAC GAAGAGATTC
    CTGCAAGATT ATTACAGGGC CATGGAATGT AAAATTCCGA TGGATGACAC TAAGAAATTC
    ACTCGAGTCC CACTGGTCCG ACTGGATAGT AGAGATATTT TGGAAAGCCA GCTTAGAATT
    CCCCTGGTTC CTCGAAGATC CATTTCTCCA GAGTCAGCTT TATCCAAACC AAAAACAAAA
    AGCATCCTGA AGGGCAAGAT CGATTACACG CTGGAAAGTG TAGAGTTAAA CTTTGAGACT
    GATGAGAAGA TGGTGATGGA TACGTGGCAG CAGTCTTCTT TGGCAGTATC TCACATGGTG
    GCTGCTGAAA TTCATCAGAG GCTCATGGAA GAAGAAAAAG AAAACCAGCC AGCAGAAACA
    AAAGAAAAAC TTCCTCAGGC AGCTGCAAAT AAAAAAGTGA AAAAGGAGCC TCCGCCACCG
    CCACCACCAC CCAAGAAAAA GAAAGAGGAC AAAAAAGGAA AAGGTAAATC CCCACCTATG
    GCAGAAGCTA CTCCTGTAGT AGTGCCAGCA GAAGAGACTG AGGAAGTGGA ATTAAAAAAT
    GAACTGAAGC TCAGAATAAA GGAAGAGCAT CTTGCTGCCC TGCAATTTGA AGAGATAGCT
    ACACAGTTCC GACTTGAACT GATAAAGGTA AAAGCATTGG CTTTCCTGGA AGAGTTAGTA
    ATAAAGGCGG TTGATGTATA TAGACTCATG GACAAGTGGC TCGGTGAGAG GTACTTGAAT
    GAAATGGCCA GTGTGGAAAA ACTAACTGGG GTAGCTCGCT ATCACATTGA AACATCAACC
    AAAATTCAGA ATGAACTTTA TTTAGACCAA GAAGACTTCT TCATTAATGG CGATATCAAA
    GTCTTTCCAG ATCCACCTCC TCCGGTACGC CCTCCGCCTG TGGAAAAGGA GGAAAATGGC
    ACCCTGACCA TGGAGCAGCT TGACAACCTC CGGGATCAGT TCTTAGATAT AGCGCCCAAA
    GGTATAATAG GAAATAAAGC ATTTGCTGAC ATTCTGCTGG ATTTGGTAAC TCTGAACCTT
    GGAACAAACA ACTTTCCTAC TAGTTGGATG AACCTCACTC AACCGGAATT GCAGGAGTTA
    GCATCTTTGT TGGTAACAAA CTCGGAGTCC GTGGACTGGC GGAAATTCTT GTTAGTAGTA
    GCGCTGCCTT GGCCCATCCC CCTGGAGGAG GAGCTTCTGG AGACCCTGCA GAGGTTCAAG
    GCCCTGGATG CGGAGCAGCT GGGCACCGTC ACTTATGAAC AGTATAAGCA GGCTGGCCTG
    TGGTTTACAG GAGATGAAGA TATCAAAATT CCAGAAAATC CTCTTGAACC CCTGCCATTC
    AATAGGCAGG AGCATCTTAT AGAGTTTTTC TTTAGGTTGT TTGCTGACTG CGAGAAGGTT
    CCACCCCAGC TTGACTACAC ACAGATGCTG CTTTATTTTG CTTGCCATCC AGACAGCACA
    GAAGGGGTCT ACAGGGCCCT CAGCGTAGCC ATTGGGACTC ACGTCTTCCA GCCAATTGAA
    ATGCCTCATC CTGTTGCAGC AAAGACCTCT TCCTTTACTG ATATGAGTCC CATTGAGGAA
    TATCCTGAAC TCGAGGACAA CTTTATGAGT GAGGAAAGGG AATTACAAGA GGACGGGGAG
    GAAAAGGAAG AAGAAATCCC TGAAAATGCA AATACGGAAA TGATTTCCAT GCAAACATTA
    CTCCAAGTGT TCCGAGGAGG AAATGAAGTA CTGGACGCTA ACAGATTTGC CAGCCACCAA
    AAGATAGAGA ACATTTATTT AGAGAACTTC ATAAAAGTGT TTCAGGACCT AGGTTCCAAG
    AACCTTGAGC CAATTGAAGT TGCTGTTCTT CTGAAGCATC CTTTTATTCA AGACCTGATT
    TCAAGTTATC CAGACTATAA GATCCCTGGT GCTTCTGCCG AGTCGGCTTC TTCAACCCTC
    CCCCGTCCTG GTCCTTCATC TCTCCTCCCT GTCATCACTT CACCCTGGAA TCTGACAAGA
    GGACAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OSm32429
    Clone ID Related Accession (Same CDS sequence) XM_012097083.2
    Accession Version XM_012097083.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5277bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-16
    Organism Ovis aries(sheep)
    Product sperm flagellar protein 2 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014639025.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012097083.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGTCGGAAA TCCTGTGCCA GTGGCTCAAC CAGGAATTGA AGGTGTCCCA GACCGTGAGT 
    CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAA TTGGAGAAGT TTTGCACAAG
    TTTGCACTTC AGGATGATTT TGCAGACTTT TCAGAAAGCA GGATTTCCAG TGCCAGACTT
    AACAATTTTT CTCGCTTGGA GCCCACACTT CACCTTTTGG GTGTGCAATT TGATCAGAAT
    GTGGCCCACA ATATCATCAC AGAGAAGCCT GGGGCTGCCA CGAAACTCTT ATATCAGTTG
    TACATTGCTC TCCAGAGGAA AAAGAAAGGT GTTCAGACTA GAGTGGAAAT GCCAACTGTA
    CAGCCCCTGA TGAGCACAAA AGTTCAAAAC ATGAAAAGCG AGGCTTTCCG AGATCAACGC
    TTGAGCAAAA GACGACAAAA TGAAATCATG GCCAAAATCC AAGCAGCTAT CATACAGATT
    CCTAAACCCA CATCAAATCG TACTCTGAAA ACATTTGACG CCCAAAAATT AATGAAAAAG
    AAAAAAGAGG CAGAGGATGT GGCCAATGAA ATTAAGAAGT TTGAAGCATT AATAAAAAAG
    GATCTCCAGG CAAAAGAAAG TGCATCCAAG ACTTCTCTAG ATACAGCAGG CCAGACAACC
    GCTGATTTGT TAAACACTTA TTCAGATGAT GACTACATTA AAAAAATCCA AAAACGCCTT
    GAAGAAGATG CTTTTGCACG AGAGCAAAGG GAGAAAAGAC GGCGGAGACT CTTAATGGAT
    CAGTTAATAG CTCATGAAGC CCAAGAGGAG GCTTATCGGG AGGAGCAGTT GATCAACCGG
    CTGATGCGAC AGTCCCAGCA GGAACGCAGG ATCGCTGTGC AGCTCATGCA CGTTAGGCAT
    GAAAAGGAAG TTTTATGGCA AAACAGAATT TTCAGAGAAA AACAATATGA GGAAAGACGG
    CTTAAAGACT TCCAGGATGC TCTTGATCGA GAAGCGGCTT TAGCCAGACA AGCCAAGATT
    GATTTTGAAG AACAAGCCCT CAGAGATAAG GAAATTCATG AGCAGATCGC CATAGAAAGA
    GCTCAAGCTC GTTACAAAAA GCATTATTCA ATATGTGCAG AAATTTTGGA TCAGATACTT
    GATCTGTCCA CTAAGGTGGC AGACTATCGA TTGTTGACAA ATAACCTGAT CCCATATAAG
    TTGATGCATG ATTGGAAGGA ACTGTTTTTC CATGGAAAAC CCATATATGA GCAAGCCTCT
    CTTAGACATT TACCAGCTAA GCTCTCTGCA GAACACCTTG TAGAAATGGA GAAAAGGAAC
    TTGCTAGATA ACAATGATTA TGAAGAATAT AAGAACATGG TTGGAGAATG GGCCTTACCA
    GAAGAAATGG TTGAAAACTT ACCACCCTCC AACAATTGCA TATTGGGCCA TGTGATTCAC
    AGACTAATTG AAAGTTCTCT TCCTCCTCAA GTTGAATCTA CAGTACCTGA GTTACCTTCA
    TTTGCTATAA AAGGATGCTT GTTGGGGAAA ACCTTTAGTG GAAAAACTAC TGCTCTAAAG
    GCTCTAGAGA AAAGCTTTCC TATTCAGGTA CTTTCTATTG ACACTCTTGT TCAAGAAGCT
    ATCCAAGCAT TTCATGACAA TGAAAGTATC AGCGGGGCCC ATGGACTTCA GAAAGCACCT
    GAAGAAAAGG TTTTACCAGT TAGGCGAAAG AGTGGTAAAG AAAACCAGGA CGTGCAAGAT
    GAAGCACTAC CAGAAAGAGA AGATAAAACC AGACTTGATA CCGATGCTAG AAAAGCAGCA
    AAAGCTGAAG AAGTCGTAGC GAGGGATAGT TTCTCTGAAC TCACTGTGCG TGCTCAGCTT
    GGTGCAAAAT CAGAAATGCT GCTGAAGAAA GGAAAGAGCA TATCTGATAT ATTGCTAGTC
    AGCATCATGG TAAATGCCAT TAATCAGATA CCTATGTATA AAGGCTGGAT CCTGGATGGC
    TTTCCAATGA CATTAAACCA AGCAAAACTT CTGGAGGAAG CACTCACAGG CTACAACAGA
    AACCTCATAG AACTGGAGGG AAAGAAATCA CAAACATCCA CATTAGCTGT TGACCCTACA
    GCTTCCAAAG AAGTGCCTCT TCCCCCTTCT GCATTTGATT TTGTCATGTT ATTAGACATT
    TCAGATAATT CCTCACTGAA TCGCATGAAT GACATCATGG CTAAAGCATT TTCAAGTGAA
    GCTCCTCGTG AAGATATCAA TCAGCGTGTA GCTGCTAAAA ACAAAGATAC AGATGAGGAC
    CAGAATTTAA GAGATCAAAT ACAGCACAGA ATTGTAGGCT TCCTGGACAA CTGGCCTTCG
    TTGGAGCAGT GGTTTACAGA GCCAGAAAAC ATTTTGATAA AAATCAATGC TGAAGTAGAT
    AAACAGTCAT TGTTCCAAAA AGTAAAGGAA ATGATTATGA CTGAAATAAT TAAAAAAGAA
    AATAAAGTGA AAAAGAAATT AGAAGAAAAG GAAGCTGAGA GAAAAGAAGA AGTTTCCCTG
    AGTGAGAAGA CATCCCCTCC TCCTCCTCCC CCTCCGGAAC CAGAGAAAGA GAAGGAAGTC
    CACCTTCCAC AGGAGCGGTC CAAAACTCCT GCTGCAAAAG GAAAGCAAGC ATCAGAACCC
    CTGCATGGTA ATCGAGAACC TCTTCAGGAA GGAAAAGGGA AGAAAAGTGA AACTTCACTC
    AAAAGAAAAG GTTCTCCTAG AGGAAAATCG CTAGGAGGGA AGATTCCACT CAAGAAATCA
    CCTGCCGAGT CTACAGATAT TTCACCGGTC CCGGTAGTGC CAGCCCCATC GAAGCCCGGA
    TCAGAAGAGT GGGTGTATGT AAATGAACCA ATTCCTGAGG AAATACCATC ATTTTTAGTA
    CCATACTGGA AATTGATAGA AAACTCCTAT ATAAATTCCA TCAAAACAGT ACTCAGGGAG
    TTGAGAGAAG GCCAGCATAG TGTACTTGCT TACTTATATG ATATTAGAAC AGGCTTCCAG
    CAGTTTCTGA AGCGTCCAGA CCACAAGCAA GACTTCGTGT CTCAGTGGCA GGCGGATTTC
    AACTCCCTTC CTGATGACCT GTGGGATGAT GAGGAAACAA AGGCTGAGCT GCACCAAAGA
    GTGAATGATT TACGGGACCG CCTGTGGGAC ATTTGCGATG CCCGTAAGGA AGAAGCAGAG
    CAGGAGCGTC TGGACATCAT TAACGAGAGC TGGTTGCAGG ACTCAATGGG AATTGCCATG
    AACCATTTCT TCTCACTGAT GCAGGCAGAA CTGAACCGTT TCCAAGATAC GAAGAGATTC
    CTGCAAGATT ATTACAGGGC CATGGAATGT AAAATTCCGA TGGATGACAC TAAGAAATTC
    ACTCGAGTCC CACTGGTCCG ACTGGATAGT AGAGATATTT TGGAAAGCCA GCTTAGAATT
    CCCCTGGTTC CTCGAAGATC CATTTCTCCA GAGTCAGCTT TATCCAAACC AAAAACAAAA
    AGCATCCTGA AGGGCAAGAT CGATTACACG CTGGAAAGTG TAGAGTTAAA CTTTGAGACT
    GATGAGAAGA TGGTGATGGA TACGTGGCAG CAGTCTTCTT TGGCAGTATC TCACATGGTG
    GCTGCTGAAA TTCATCAGAG GCTCATGGAA GAAGAAAAAG AAAACCAGCC AGCAGAAACA
    AAAGAAAAAC TTCCTCAGGC AGCTGCAAAT AAAAAAGTGA AAAAGGAGCC TCCGCCACCG
    CCACCACCAC CCAAGAAAAA GAAAGAGGAC AAAAAAGGAA AAGGTAAATC CCCACCTATG
    GCAGAAGCTA CTCCTGTAGT AGTGCCAGCA GAAGAGACTG AGGAAGTGGA ATTAAAAAAT
    GAACTGAAGC TCAGAATAAA GGAAGAGCAT CTTGCTGCCC TGCAATTTGA AGAGATAGCT
    ACACAGTTCC GACTTGAACT GATAAAGGTA AAAGCATTGG CTTTCCTGGA AGAGTTAGTA
    ATAAAGGCGG TTGATGTATA TAGACTCATG GACAAGTGGC TCGGTGAGAG GTACTTGAAT
    GAAATGGCCA GTGTGGAAAA ACTAACTGGG GTAGCTCGCT ATCACATTGA AACATCAACC
    AAAATTCAGA ATGAACTTTA TTTAGACCAA GAAGACTTCT TCATTAATGG CGATATCAAA
    GTCTTTCCAG ATCCACCTCC TCCGGTACGC CCTCCGCCTG TGGAAAAGGA GGAAAATGGC
    ACCCTGACCA TGGAGCAGCT TGACAACCTC CGGGATCAGT TCTTAGATAT AGCGCCCAAA
    GGTATAATAG GAAATAAAGC ATTTGCTGAC ATTCTGCTGG ATTTGGTAAC TCTGAACCTT
    GGAACAAACA ACTTTCCTAC TAGTTGGATG AACCTCACTC AACCGGAATT GCAGGAGTTA
    GCATCTTTGT TGGTAACAAA CTCGGAGTCC GTGGACTGGC GGAAATTCTT GTTAGTAGTA
    GCGCTGCCTT GGCCCATCCC CCTGGAGGAG GAGCTTCTGG AGACCCTGCA GAGGTTCAAG
    GCCCTGGATG CGGAGCAGCT GGGCACCGTC ACTTATGAAC AGTATAAGCA GGCTGGCCTG
    TGGTTTACAG GAGATGAAGA TATCAAAATT CCAGAAAATC CTCTTGAACC CCTGCCATTC
    AATAGGCAGG AGCATCTTAT AGAGTTTTTC TTTAGGTTGT TTGCTGACTG CGAGAAGGTT
    CCACCCCAGC TTGACTACAC ACAGATGCTG CTTTATTTTG CTTGCCATCC AGACAGCACA
    GAAGGGGTCT ACAGGGCCCT CAGCGTAGCC ATTGGGACTC ACGTCTTCCA GCCAATTGAA
    ATGCCTCATC CTGTTGCAGC AAAGACCTCT TCCTTTACTG ATATGAGTCC CATTGAGGAA
    TATCCTGAAC TCGAGGACAA CTTTATGAGT GAGGAAAGGG AATTACAAGA GGACGGGGAG
    GAAAAGGAAG AAGAAATCCC TGAAAATGCA AATACGGAAA TGATTTCCAT GCAAACATTA
    CTCCAAGTGT TCCGAGGAGG AAATGAAGTA CTGGACGCTA ACAGATTTGC CAGCCACCAA
    AAGATAGAGA ACATTTATTT AGAGAACTTC ATAAAAGTGT TTCAGGACCT AGGTTCCAAG
    AACCTTGAGC CAATTGAAGT TGCTGTTCTT CTGAAGCATC CTTTTATTCA AGACCTGATT
    TCAAGTTATC CAGACTATAA GATCCCTGAT GTTAAGATAA TTCTCCAAAG GAGTGAGCAT
    GTACAAGGAA GTGATGAAGA GAGGTCTCCT TCAAGACTTA CAGAGGAAAA GAAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011952473.1
    CDS493..5769
    Translation

    Target ORF information:

    RefSeq Version XM_012097083.2
    Organism Ovis aries(sheep)
    Definition Ovis aries sperm flagellar 2 (SPEF2), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012097083.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGTCGGAAA TCCTGTGCCA GTGGCTCAAC CAGGAATTGA AGGTGTCCCA GACCGTGAGT 
    CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAA TTGGAGAAGT TTTGCACAAG
    TTTGCACTTC AGGATGATTT TGCAGACTTT TCAGAAAGCA GGATTTCCAG TGCCAGACTT
    AACAATTTTT CTCGCTTGGA GCCCACACTT CACCTTTTGG GTGTGCAATT TGATCAGAAT
    GTGGCCCACA ATATCATCAC AGAGAAGCCT GGGGCTGCCA CGAAACTCTT ATATCAGTTG
    TACATTGCTC TCCAGAGGAA AAAGAAAGGT GTTCAGACTA GAGTGGAAAT GCCAACTGTA
    CAGCCCCTGA TGAGCACAAA AGTTCAAAAC ATGAAAAGCG AGGCTTTCCG AGATCAACGC
    TTGAGCAAAA GACGACAAAA TGAAATCATG GCCAAAATCC AAGCAGCTAT CATACAGATT
    CCTAAACCCA CATCAAATCG TACTCTGAAA ACATTTGACG CCCAAAAATT AATGAAAAAG
    AAAAAAGAGG CAGAGGATGT GGCCAATGAA ATTAAGAAGT TTGAAGCATT AATAAAAAAG
    GATCTCCAGG CAAAAGAAAG TGCATCCAAG ACTTCTCTAG ATACAGCAGG CCAGACAACC
    GCTGATTTGT TAAACACTTA TTCAGATGAT GACTACATTA AAAAAATCCA AAAACGCCTT
    GAAGAAGATG CTTTTGCACG AGAGCAAAGG GAGAAAAGAC GGCGGAGACT CTTAATGGAT
    CAGTTAATAG CTCATGAAGC CCAAGAGGAG GCTTATCGGG AGGAGCAGTT GATCAACCGG
    CTGATGCGAC AGTCCCAGCA GGAACGCAGG ATCGCTGTGC AGCTCATGCA CGTTAGGCAT
    GAAAAGGAAG TTTTATGGCA AAACAGAATT TTCAGAGAAA AACAATATGA GGAAAGACGG
    CTTAAAGACT TCCAGGATGC TCTTGATCGA GAAGCGGCTT TAGCCAGACA AGCCAAGATT
    GATTTTGAAG AACAAGCCCT CAGAGATAAG GAAATTCATG AGCAGATCGC CATAGAAAGA
    GCTCAAGCTC GTTACAAAAA GCATTATTCA ATATGTGCAG AAATTTTGGA TCAGATACTT
    GATCTGTCCA CTAAGGTGGC AGACTATCGA TTGTTGACAA ATAACCTGAT CCCATATAAG
    TTGATGCATG ATTGGAAGGA ACTGTTTTTC CATGGAAAAC CCATATATGA GCAAGCCTCT
    CTTAGACATT TACCAGCTAA GCTCTCTGCA GAACACCTTG TAGAAATGGA GAAAAGGAAC
    TTGCTAGATA ACAATGATTA TGAAGAATAT AAGAACATGG TTGGAGAATG GGCCTTACCA
    GAAGAAATGG TTGAAAACTT ACCACCCTCC AACAATTGCA TATTGGGCCA TGTGATTCAC
    AGACTAATTG AAAGTTCTCT TCCTCCTCAA GTTGAATCTA CAGTACCTGA GTTACCTTCA
    TTTGCTATAA AAGGATGCTT GTTGGGGAAA ACCTTTAGTG GAAAAACTAC TGCTCTAAAG
    GCTCTAGAGA AAAGCTTTCC TATTCAGGTA CTTTCTATTG ACACTCTTGT TCAAGAAGCT
    ATCCAAGCAT TTCATGACAA TGAAAGTATC AGCGGGGCCC ATGGACTTCA GAAAGCACCT
    GAAGAAAAGG TTTTACCAGT TAGGCGAAAG AGTGGTAAAG AAAACCAGGA CGTGCAAGAT
    GAAGCACTAC CAGAAAGAGA AGATAAAACC AGACTTGATA CCGATGCTAG AAAAGCAGCA
    AAAGCTGAAG AAGTCGTAGC GAGGGATAGT TTCTCTGAAC TCACTGTGCG TGCTCAGCTT
    GGTGCAAAAT CAGAAATGCT GCTGAAGAAA GGAAAGAGCA TATCTGATAT ATTGCTAGTC
    AGCATCATGG TAAATGCCAT TAATCAGATA CCTATGTATA AAGGCTGGAT CCTGGATGGC
    TTTCCAATGA CATTAAACCA AGCAAAACTT CTGGAGGAAG CACTCACAGG CTACAACAGA
    AACCTCATAG AACTGGAGGG AAAGAAATCA CAAACATCCA CATTAGCTGT TGACCCTACA
    GCTTCCAAAG AAGTGCCTCT TCCCCCTTCT GCATTTGATT TTGTCATGTT ATTAGACATT
    TCAGATAATT CCTCACTGAA TCGCATGAAT GACATCATGG CTAAAGCATT TTCAAGTGAA
    GCTCCTCGTG AAGATATCAA TCAGCGTGTA GCTGCTAAAA ACAAAGATAC AGATGAGGAC
    CAGAATTTAA GAGATCAAAT ACAGCACAGA ATTGTAGGCT TCCTGGACAA CTGGCCTTCG
    TTGGAGCAGT GGTTTACAGA GCCAGAAAAC ATTTTGATAA AAATCAATGC TGAAGTAGAT
    AAACAGTCAT TGTTCCAAAA AGTAAAGGAA ATGATTATGA CTGAAATAAT TAAAAAAGAA
    AATAAAGTGA AAAAGAAATT AGAAGAAAAG GAAGCTGAGA GAAAAGAAGA AGTTTCCCTG
    AGTGAGAAGA CATCCCCTCC TCCTCCTCCC CCTCCGGAAC CAGAGAAAGA GAAGGAAGTC
    CACCTTCCAC AGGAGCGGTC CAAAACTCCT GCTGCAAAAG GAAAGCAAGC ATCAGAACCC
    CTGCATGGTA ATCGAGAACC TCTTCAGGAA GGAAAAGGGA AGAAAAGTGA AACTTCACTC
    AAAAGAAAAG GTTCTCCTAG AGGAAAATCG CTAGGAGGGA AGATTCCACT CAAGAAATCA
    CCTGCCGAGT CTACAGATAT TTCACCGGTC CCGGTAGTGC CAGCCCCATC GAAGCCCGGA
    TCAGAAGAGT GGGTGTATGT AAATGAACCA ATTCCTGAGG AAATACCATC ATTTTTAGTA
    CCATACTGGA AATTGATAGA AAACTCCTAT ATAAATTCCA TCAAAACAGT ACTCAGGGAG
    TTGAGAGAAG GCCAGCATAG TGTACTTGCT TACTTATATG ATATTAGAAC AGGCTTCCAG
    CAGTTTCTGA AGCGTCCAGA CCACAAGCAA GACTTCGTGT CTCAGTGGCA GGCGGATTTC
    AACTCCCTTC CTGATGACCT GTGGGATGAT GAGGAAACAA AGGCTGAGCT GCACCAAAGA
    GTGAATGATT TACGGGACCG CCTGTGGGAC ATTTGCGATG CCCGTAAGGA AGAAGCAGAG
    CAGGAGCGTC TGGACATCAT TAACGAGAGC TGGTTGCAGG ACTCAATGGG AATTGCCATG
    AACCATTTCT TCTCACTGAT GCAGGCAGAA CTGAACCGTT TCCAAGATAC GAAGAGATTC
    CTGCAAGATT ATTACAGGGC CATGGAATGT AAAATTCCGA TGGATGACAC TAAGAAATTC
    ACTCGAGTCC CACTGGTCCG ACTGGATAGT AGAGATATTT TGGAAAGCCA GCTTAGAATT
    CCCCTGGTTC CTCGAAGATC CATTTCTCCA GAGTCAGCTT TATCCAAACC AAAAACAAAA
    AGCATCCTGA AGGGCAAGAT CGATTACACG CTGGAAAGTG TAGAGTTAAA CTTTGAGACT
    GATGAGAAGA TGGTGATGGA TACGTGGCAG CAGTCTTCTT TGGCAGTATC TCACATGGTG
    GCTGCTGAAA TTCATCAGAG GCTCATGGAA GAAGAAAAAG AAAACCAGCC AGCAGAAACA
    AAAGAAAAAC TTCCTCAGGC AGCTGCAAAT AAAAAAGTGA AAAAGGAGCC TCCGCCACCG
    CCACCACCAC CCAAGAAAAA GAAAGAGGAC AAAAAAGGAA AAGGTAAATC CCCACCTATG
    GCAGAAGCTA CTCCTGTAGT AGTGCCAGCA GAAGAGACTG AGGAAGTGGA ATTAAAAAAT
    GAACTGAAGC TCAGAATAAA GGAAGAGCAT CTTGCTGCCC TGCAATTTGA AGAGATAGCT
    ACACAGTTCC GACTTGAACT GATAAAGGTA AAAGCATTGG CTTTCCTGGA AGAGTTAGTA
    ATAAAGGCGG TTGATGTATA TAGACTCATG GACAAGTGGC TCGGTGAGAG GTACTTGAAT
    GAAATGGCCA GTGTGGAAAA ACTAACTGGG GTAGCTCGCT ATCACATTGA AACATCAACC
    AAAATTCAGA ATGAACTTTA TTTAGACCAA GAAGACTTCT TCATTAATGG CGATATCAAA
    GTCTTTCCAG ATCCACCTCC TCCGGTACGC CCTCCGCCTG TGGAAAAGGA GGAAAATGGC
    ACCCTGACCA TGGAGCAGCT TGACAACCTC CGGGATCAGT TCTTAGATAT AGCGCCCAAA
    GGTATAATAG GAAATAAAGC ATTTGCTGAC ATTCTGCTGG ATTTGGTAAC TCTGAACCTT
    GGAACAAACA ACTTTCCTAC TAGTTGGATG AACCTCACTC AACCGGAATT GCAGGAGTTA
    GCATCTTTGT TGGTAACAAA CTCGGAGTCC GTGGACTGGC GGAAATTCTT GTTAGTAGTA
    GCGCTGCCTT GGCCCATCCC CCTGGAGGAG GAGCTTCTGG AGACCCTGCA GAGGTTCAAG
    GCCCTGGATG CGGAGCAGCT GGGCACCGTC ACTTATGAAC AGTATAAGCA GGCTGGCCTG
    TGGTTTACAG GAGATGAAGA TATCAAAATT CCAGAAAATC CTCTTGAACC CCTGCCATTC
    AATAGGCAGG AGCATCTTAT AGAGTTTTTC TTTAGGTTGT TTGCTGACTG CGAGAAGGTT
    CCACCCCAGC TTGACTACAC ACAGATGCTG CTTTATTTTG CTTGCCATCC AGACAGCACA
    GAAGGGGTCT ACAGGGCCCT CAGCGTAGCC ATTGGGACTC ACGTCTTCCA GCCAATTGAA
    ATGCCTCATC CTGTTGCAGC AAAGACCTCT TCCTTTACTG ATATGAGTCC CATTGAGGAA
    TATCCTGAAC TCGAGGACAA CTTTATGAGT GAGGAAAGGG AATTACAAGA GGACGGGGAG
    GAAAAGGAAG AAGAAATCCC TGAAAATGCA AATACGGAAA TGATTTCCAT GCAAACATTA
    CTCCAAGTGT TCCGAGGAGG AAATGAAGTA CTGGACGCTA ACAGATTTGC CAGCCACCAA
    AAGATAGAGA ACATTTATTT AGAGAACTTC ATAAAAGTGT TTCAGGACCT AGGTTCCAAG
    AACCTTGAGC CAATTGAAGT TGCTGTTCTT CTGAAGCATC CTTTTATTCA AGACCTGATT
    TCAAGTTATC CAGACTATAA GATCCCTGAT GTTAAGATAA TTCTCCAAAG GAGTGAGCAT
    GTACAAGGAA GTGATGAAGA GAGGTCTCCT TCAAGACTTA CAGAGGAAAA GAAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OSm32430
    Clone ID Related Accession (Same CDS sequence) XM_012097084.2
    Accession Version XM_012097084.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5250bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-16
    Organism Ovis aries(sheep)
    Product sperm flagellar protein 2 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014639025.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012097084.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGAAAAGGA AGTTTTATGG CAAAACAGAA TTTTCAGAGA AAAACAATAT GAGGAAAGAC 
    GGCTTAAAGA CTTCCAGGAT GCTCTTGATC GAGAAGCGCC TGATCCCATA TAAGTTGATG
    CATGATTGGA AGGAACTGTT TTTCCATGGA AAACCCATAT ATGAGCAAGC CTCTCTTAGA
    CATTTACCAG CTAAGCTCTC TGCAGAACAC CTTGTAGAAA TGGAGAAAAG GAACTTGCTA
    GATAACAATG ATTATGAAGA ATATAAGAAC ATGGTTGGAG AATGGGCCTT ACCAGAAGAA
    ATGGTTGAAA ACTTACCACC CTCCAACAAT TGCATATTGG GCCATGTGAT TCACAGACTA
    ATTGAAAGTT CTCTTCCTCC TCAAGTTGAA TCTACAGTAC CTGAGTTACC TTCATTTGCT
    ATAAAAGGAT GCTTGTTGGG GAAAACCTTT AGTGGAAAAA CTACTGCTCT AAAGGCTCTA
    GAGAAAAGCT TTCCTATTCA GGTACTTTCT ATTGACACTC TTGTTCAAGA AGCTATCCAA
    GCATTTCATG ACAATGAAAG TATCAGCGGG GCCCATGGAC TTCAGAAAGC ACCTGAAGAA
    AAGGTTTTAC CAGTTAGGCG AAAGAGTGGT AAAGAAAACC AGGACGTGCA AGATGAAGCA
    CTACCAGAAA GAGAAGATAA AACCAGACTT GATACCGATG CTAGAAAAGC AGCAAAAGCT
    GAAGAAGTCG TAGCGAGGGA TAGTTTCTCT GAACTCACTG TGCGTGCTCA GCTTGGTGCA
    AAATCAGAAA TGCTGCTGAA GAAAGGAAAG AGCATATCTG ATATATTGCT AGTCAGCATC
    ATGGTAAATG CCATTAATCA GATACCTATG TATAAAGGCT GGATCCTGGA TGGCTTTCCA
    ATGACATTAA ACCAAGCAAA ACTTCTGGAG GAAGCACTCA CAGGCTACAA CAGAAACCTC
    ATAGAACTGG AGGGAAAGAA ATCACAAACA TCCACATTAG CTGTTGACCC TACAGCTTCC
    AAAGAAGTGC CTCTTCCCCC TTCTGCATTT GATTTTGTCA TGTTATTAGA CATTTCAGAT
    AATTCCTCAC TGAATCGCAT GAATGACATC ATGGCTAAAG CATTTTCAAG TGAAGCTCCT
    CGTGAAGATA TCAATCAGCG TGTAGCTGCT AAAAACAAAG ATACAGATGA GGACCAGAAT
    TTAAGAGATC AAATACAGCA CAGAATTGTA GGCTTCCTGG ACAACTGGCC TTCGTTGGAG
    CAGTGGTTTA CAGAGCCAGA AAACATTTTG ATAAAAATCA ATGCTGAAGT AGATAAACAG
    TCATTGTTCC AAAAAGTAAA GGAAATGATT ATGACTGAAA TAATTAAAAA AGAAAATAAA
    GTGAAAAAGA AATTAGAAGA AAAGGAAGCT GAGAGAAAAG AAGAAGTTTC CCTGAGTGAG
    AAGACATCCC CTCCTCCTCC TCCCCCTCCG GAACCAGAGA AAGAGAAGGA AGTCCACCTT
    CCACAGGAGC GGTCCAAAAC TCCTGCTGCA AAAGGAAAGC AAGCATCAGA ACCCCTGCAT
    GGTAATCGAG AACCTCTTCA GGAAGGAAAA GGGAAGAAAA GTGAAACTTC ACTCAAAAGA
    AAAGGTTCTC CTAGAGGAAA ATCGCTAGGA GGGAAGATTC CACTCAAGAA ATCACCTGCC
    GAGTCTACAG ATATTTCACC GGTCCCGGTA GTGCCAGCCC CATCGAAGCC CGGATCAGAA
    GAGTGGGTGT ATGTAAATGA ACCAATTCCT GAGGAAATAC CATCATTTTT AGTACCATAC
    TGGAAATTGA TAGAAAACTC CTATATAAAT TCCATCAAAA CAGTACTCAG GGAGTTGAGA
    GAAGGCCAGC ATAGTGTACT TGCTTACTTA TATGATATTA GAACAGGCTT CCAGCAGTTT
    CTGAAGCGTC CAGACCACAA GCAAGACTTC GTGTCTCAGT GGCAGGCGGA TTTCAACTCC
    CTTCCTGATG ACCTGTGGGA TGATGAGGAA ACAAAGGCTG AGCTGCACCA AAGAGTGAAT
    GATTTACGGG ACCGCCTGTG GGACATTTGC GATGCCCGTA AGGAAGAAGC AGAGCAGGAG
    CGTCTGGACA TCATTAACGA GAGCTGGTTG CAGGACTCAA TGGGAATTGC CATGAACCAT
    TTCTTCTCAC TGATGCAGGC AGAACTGAAC CGTTTCCAAG ATACGAAGAG ATTCCTGCAA
    GATTATTACA GGGCCATGGA ATGTAAAATT CCGATGGATG ACACTAAGAA ATTCACTCGA
    GTCCCACTGG TCCGACTGGA TAGTAGAGAT ATTTTGGAAA GCCAGCTTAG AATTCCCCTG
    GTTCCTCGAA GATCCATTTC TCCAGAGTCA GCTTTATCCA AACCAAAAAC AAAAAGCATC
    CTGAAGGGCA AGATCGATTA CACGCTGGAA AGTGTAGAGT TAAACTTTGA GACTGATGAG
    AAGATGGTGA TGGATACGTG GCAGCAGTCT TCTTTGGCAG TATCTCACAT GGTGGCTGCT
    GAAATTCATC AGAGGCTCAT GGAAGAAGAA AAAGAAAACC AGCCAGCAGA AACAAAAGAA
    AAACTTCCTC AGGCAGCTGC AAATAAAAAA GTGAAAAAGG AGCCTCCGCC ACCGCCACCA
    CCACCCAAGA AAAAGAAAGA GGACAAAAAA GGAAAAGGTA AATCCCCACC TATGGCAGAA
    GCTACTCCTG TAGTAGTGCC AGCAGAAGAG ACTGAGGAAG TGGAATTAAA AAATGAACTG
    AAGCTCAGAA TAAAGGAAGA GCATCTTGCT GCCCTGCAAT TTGAAGAGAT AGCTACACAG
    TTCCGACTTG AACTGATAAA GGTAAAAGCA TTGGCTTTCC TGGAAGAGTT AGTAATAAAG
    GCGGTTGATG TATATAGACT CATGGACAAG TGGCTCGGTG AGAGGTACTT GAATGAAATG
    GCCAGTGTGG AAAAACTAAC TGGGGTAGCT CGCTATCACA TTGAAACATC AACCAAAATT
    CAGAATGAAC TTTATTTAGA CCAAGAAGAC TTCTTCATTA ATGGCGATAT CAAAGTCTTT
    CCAGATCCAC CTCCTCCGGT ACGCCCTCCG CCTGTGGAAA AGGAGGAAAA TGGCACCCTG
    ACCATGGAGC AGCTTGACAA CCTCCGGGAT CAGTTCTTAG ATATAGCGCC CAAAGGCAGG
    GATCCTCCAG ACAATGTGAG TGGATTCACC AAGAAGCAGA AAAGGCCTCA GAAGCTTGAC
    CATTACCTCC CAAAGAGGAA CAATCAATTT TTGAAAATTT GGCTTAAAAA ATACCCTTGG
    CTTGTATACG ATGAGATACT AAATCTTATG TTCTGCGGCC TGTGTCGTAA ACATGGAGTG
    ACATCAAAGG GAAATCAGGT CAGTTTTTTT TACGGCACGG ATAACTTCAG GACTGAATTT
    CTCAACGCGC ATCATCTGAG TGAGGCTCAC GCCAAGGCGT CCCTCATGGA AGCCACAAGT
    GGTTCTCCAG GGAGCAGAGC TACCACGGAG CTGATGGTGA GGACCATGAG CAAGGTGACC
    CTTGGCAGGG TGGAGAACCT GTTCCGGTCC TGCCACGCCA TCGCCAAGAC CGGACGTCCC
    CTCAAAGACT TCCTCTGGAT GTGTTCGCTG GACGATATGA AGGGAATTGA TATCGGCCCG
    GTGTTCAGAA CCAGTAAATC GGCCAGAACT TTTACCTACT TTATTGCTGA AGTGGAACGG
    AGAACTCTAA GAGAGAAGTT AGAAAAAAGT CAGTTCTTCT CCGTCCTCAG TGATGGGATT
    GCAGACAATT CAGCTGAGGA AGCTGAACTC GTATATGTGC AGTTTGCCCG CGCAGGGAGA
    GTGCACTGCC AAATCGTCGG GGTACAGACC GTGGAGAAGA AGGAGCCCTG GGCGATAAAA
    CGTGCCATTG AGAAAACTCT GGAGAGAAAT CTTCAACTTC GACTGTCGAA CCAAGACTGG
    GCAAAGAAAC TGGTGGGTTT CGGAAGCGAT GGAGCTCCAG GTTCAGAGGG AGAAAACGGC
    AGCACAGTGG CCCTGCTTTT AAGGGAAATC CAGCCAGGTG TGCTGCCTGT TTACTGCCTT
    GCCCATCACC TCGAACTGTC TTACCAGGCG GTGTTCCAGA GCGTTCCCCT CTACAGCCGT
    GTGAAAGACC TTCTTCATAG CATGCACCGC TTCTATCACG ATTCTCCTGC TCACAGGAGT
    GCTCTGGTGA CTGCTTTCAA GGGCCTCCAC CTTCAACCTG CGATGCCCTC TCAAGTCAGA
    GGCCGGAGGT GGCTTCAGGG ATTGCAGGCT GCCCTCCAGA ACTTTCTCAA GGGATATCCA
    GCAATTGTCC AGCAGCTGCA GTCAACAAAA GAAAGCAGAA GCAACAGCAG TCATCAGAAA
    GCCGAAGAAC TCCTGGAGTC TCTCCTCCAG GCAGACATTG TCAAATTTGT CCACTTCTTG
    GTGGATGTCG TCAATGTCCT TAGCATACTG TCCCATGTCA ATCAGAACAG AAATTCCTCA
    ATTGCTGATG TATTTGCCAC CTTAGAGTCA ACACTGGAAA TGCTCCAAAT TTATCAGACA
    AGACCAGGGC CGAAAGAACG TGGGGTAGAT TCGGTCACAC ACTTTCATGG TAACTGCCTT
    GGAGGAAAGG GAAACATTTC TGACGTGAGA AACATTGTTT TAACACATCT TATCAAGAGG
    CTGCGAGGCT GTTTCAGAGA TGCCAACCAA GATGTGGTGA AGGCAACCAC GATTGGAAGC
    TTTAAACTGT GGCCGGCAAA AATAAATCAA GAATTTGGAG AAAAAGAGGT GGCCATCCTG
    ATTGCACATT ATGAACCAGT ATTAGAGGCA GCCAAGGTGA AAATTGACCA AGTAGACACA
    GAATGGAGCA TGTTGAAATT GGAGATCTAC TCTAGATTTC AGAACATTTG CAAACTGACC
    TGGGATTTTG TGAATTCTGT TTATTTACAC AAGTATCCAA ATATCCTGAC ACTCATTGAC
    CTCGTGCTGA GCCTGCCTGC AAGTTCAGCT GAGGCAGGAC GTGGCTTTAG TCAGATGAAG
    CGCATCAGGT CCCAAATGCA TGCCAAGGTC AAGGCTGACA ACATGACGGA TCTCCTGATA
    ATTCAGTTGA ATTCTCCAGA CATTAACAAC TTTGACCCTA GGAAAGCCAT TCATTTATGG
    AATACAAGAA CACCATCCTC AACTGAGGAC ACAAGACCTA ACTCAGACTG CTCATCAAAC
    TCAGAAAGCC TTAGTGAAAG CGATGAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011952474.1
    CDS231..5480
    Translation

    Target ORF information:

    RefSeq Version XM_012097084.2
    Organism Ovis aries(sheep)
    Definition Ovis aries sperm flagellar 2 (SPEF2), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012097084.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGAAAAGGA AGTTTTATGG CAAAACAGAA TTTTCAGAGA AAAACAATAT GAGGAAAGAC 
    GGCTTAAAGA CTTCCAGGAT GCTCTTGATC GAGAAGCGCC TGATCCCATA TAAGTTGATG
    CATGATTGGA AGGAACTGTT TTTCCATGGA AAACCCATAT ATGAGCAAGC CTCTCTTAGA
    CATTTACCAG CTAAGCTCTC TGCAGAACAC CTTGTAGAAA TGGAGAAAAG GAACTTGCTA
    GATAACAATG ATTATGAAGA ATATAAGAAC ATGGTTGGAG AATGGGCCTT ACCAGAAGAA
    ATGGTTGAAA ACTTACCACC CTCCAACAAT TGCATATTGG GCCATGTGAT TCACAGACTA
    ATTGAAAGTT CTCTTCCTCC TCAAGTTGAA TCTACAGTAC CTGAGTTACC TTCATTTGCT
    ATAAAAGGAT GCTTGTTGGG GAAAACCTTT AGTGGAAAAA CTACTGCTCT AAAGGCTCTA
    GAGAAAAGCT TTCCTATTCA GGTACTTTCT ATTGACACTC TTGTTCAAGA AGCTATCCAA
    GCATTTCATG ACAATGAAAG TATCAGCGGG GCCCATGGAC TTCAGAAAGC ACCTGAAGAA
    AAGGTTTTAC CAGTTAGGCG AAAGAGTGGT AAAGAAAACC AGGACGTGCA AGATGAAGCA
    CTACCAGAAA GAGAAGATAA AACCAGACTT GATACCGATG CTAGAAAAGC AGCAAAAGCT
    GAAGAAGTCG TAGCGAGGGA TAGTTTCTCT GAACTCACTG TGCGTGCTCA GCTTGGTGCA
    AAATCAGAAA TGCTGCTGAA GAAAGGAAAG AGCATATCTG ATATATTGCT AGTCAGCATC
    ATGGTAAATG CCATTAATCA GATACCTATG TATAAAGGCT GGATCCTGGA TGGCTTTCCA
    ATGACATTAA ACCAAGCAAA ACTTCTGGAG GAAGCACTCA CAGGCTACAA CAGAAACCTC
    ATAGAACTGG AGGGAAAGAA ATCACAAACA TCCACATTAG CTGTTGACCC TACAGCTTCC
    AAAGAAGTGC CTCTTCCCCC TTCTGCATTT GATTTTGTCA TGTTATTAGA CATTTCAGAT
    AATTCCTCAC TGAATCGCAT GAATGACATC ATGGCTAAAG CATTTTCAAG TGAAGCTCCT
    CGTGAAGATA TCAATCAGCG TGTAGCTGCT AAAAACAAAG ATACAGATGA GGACCAGAAT
    TTAAGAGATC AAATACAGCA CAGAATTGTA GGCTTCCTGG ACAACTGGCC TTCGTTGGAG
    CAGTGGTTTA CAGAGCCAGA AAACATTTTG ATAAAAATCA ATGCTGAAGT AGATAAACAG
    TCATTGTTCC AAAAAGTAAA GGAAATGATT ATGACTGAAA TAATTAAAAA AGAAAATAAA
    GTGAAAAAGA AATTAGAAGA AAAGGAAGCT GAGAGAAAAG AAGAAGTTTC CCTGAGTGAG
    AAGACATCCC CTCCTCCTCC TCCCCCTCCG GAACCAGAGA AAGAGAAGGA AGTCCACCTT
    CCACAGGAGC GGTCCAAAAC TCCTGCTGCA AAAGGAAAGC AAGCATCAGA ACCCCTGCAT
    GGTAATCGAG AACCTCTTCA GGAAGGAAAA GGGAAGAAAA GTGAAACTTC ACTCAAAAGA
    AAAGGTTCTC CTAGAGGAAA ATCGCTAGGA GGGAAGATTC CACTCAAGAA ATCACCTGCC
    GAGTCTACAG ATATTTCACC GGTCCCGGTA GTGCCAGCCC CATCGAAGCC CGGATCAGAA
    GAGTGGGTGT ATGTAAATGA ACCAATTCCT GAGGAAATAC CATCATTTTT AGTACCATAC
    TGGAAATTGA TAGAAAACTC CTATATAAAT TCCATCAAAA CAGTACTCAG GGAGTTGAGA
    GAAGGCCAGC ATAGTGTACT TGCTTACTTA TATGATATTA GAACAGGCTT CCAGCAGTTT
    CTGAAGCGTC CAGACCACAA GCAAGACTTC GTGTCTCAGT GGCAGGCGGA TTTCAACTCC
    CTTCCTGATG ACCTGTGGGA TGATGAGGAA ACAAAGGCTG AGCTGCACCA AAGAGTGAAT
    GATTTACGGG ACCGCCTGTG GGACATTTGC GATGCCCGTA AGGAAGAAGC AGAGCAGGAG
    CGTCTGGACA TCATTAACGA GAGCTGGTTG CAGGACTCAA TGGGAATTGC CATGAACCAT
    TTCTTCTCAC TGATGCAGGC AGAACTGAAC CGTTTCCAAG ATACGAAGAG ATTCCTGCAA
    GATTATTACA GGGCCATGGA ATGTAAAATT CCGATGGATG ACACTAAGAA ATTCACTCGA
    GTCCCACTGG TCCGACTGGA TAGTAGAGAT ATTTTGGAAA GCCAGCTTAG AATTCCCCTG
    GTTCCTCGAA GATCCATTTC TCCAGAGTCA GCTTTATCCA AACCAAAAAC AAAAAGCATC
    CTGAAGGGCA AGATCGATTA CACGCTGGAA AGTGTAGAGT TAAACTTTGA GACTGATGAG
    AAGATGGTGA TGGATACGTG GCAGCAGTCT TCTTTGGCAG TATCTCACAT GGTGGCTGCT
    GAAATTCATC AGAGGCTCAT GGAAGAAGAA AAAGAAAACC AGCCAGCAGA AACAAAAGAA
    AAACTTCCTC AGGCAGCTGC AAATAAAAAA GTGAAAAAGG AGCCTCCGCC ACCGCCACCA
    CCACCCAAGA AAAAGAAAGA GGACAAAAAA GGAAAAGGTA AATCCCCACC TATGGCAGAA
    GCTACTCCTG TAGTAGTGCC AGCAGAAGAG ACTGAGGAAG TGGAATTAAA AAATGAACTG
    AAGCTCAGAA TAAAGGAAGA GCATCTTGCT GCCCTGCAAT TTGAAGAGAT AGCTACACAG
    TTCCGACTTG AACTGATAAA GGTAAAAGCA TTGGCTTTCC TGGAAGAGTT AGTAATAAAG
    GCGGTTGATG TATATAGACT CATGGACAAG TGGCTCGGTG AGAGGTACTT GAATGAAATG
    GCCAGTGTGG AAAAACTAAC TGGGGTAGCT CGCTATCACA TTGAAACATC AACCAAAATT
    CAGAATGAAC TTTATTTAGA CCAAGAAGAC TTCTTCATTA ATGGCGATAT CAAAGTCTTT
    CCAGATCCAC CTCCTCCGGT ACGCCCTCCG CCTGTGGAAA AGGAGGAAAA TGGCACCCTG
    ACCATGGAGC AGCTTGACAA CCTCCGGGAT CAGTTCTTAG ATATAGCGCC CAAAGGCAGG
    GATCCTCCAG ACAATGTGAG TGGATTCACC AAGAAGCAGA AAAGGCCTCA GAAGCTTGAC
    CATTACCTCC CAAAGAGGAA CAATCAATTT TTGAAAATTT GGCTTAAAAA ATACCCTTGG
    CTTGTATACG ATGAGATACT AAATCTTATG TTCTGCGGCC TGTGTCGTAA ACATGGAGTG
    ACATCAAAGG GAAATCAGGT CAGTTTTTTT TACGGCACGG ATAACTTCAG GACTGAATTT
    CTCAACGCGC ATCATCTGAG TGAGGCTCAC GCCAAGGCGT CCCTCATGGA AGCCACAAGT
    GGTTCTCCAG GGAGCAGAGC TACCACGGAG CTGATGGTGA GGACCATGAG CAAGGTGACC
    CTTGGCAGGG TGGAGAACCT GTTCCGGTCC TGCCACGCCA TCGCCAAGAC CGGACGTCCC
    CTCAAAGACT TCCTCTGGAT GTGTTCGCTG GACGATATGA AGGGAATTGA TATCGGCCCG
    GTGTTCAGAA CCAGTAAATC GGCCAGAACT TTTACCTACT TTATTGCTGA AGTGGAACGG
    AGAACTCTAA GAGAGAAGTT AGAAAAAAGT CAGTTCTTCT CCGTCCTCAG TGATGGGATT
    GCAGACAATT CAGCTGAGGA AGCTGAACTC GTATATGTGC AGTTTGCCCG CGCAGGGAGA
    GTGCACTGCC AAATCGTCGG GGTACAGACC GTGGAGAAGA AGGAGCCCTG GGCGATAAAA
    CGTGCCATTG AGAAAACTCT GGAGAGAAAT CTTCAACTTC GACTGTCGAA CCAAGACTGG
    GCAAAGAAAC TGGTGGGTTT CGGAAGCGAT GGAGCTCCAG GTTCAGAGGG AGAAAACGGC
    AGCACAGTGG CCCTGCTTTT AAGGGAAATC CAGCCAGGTG TGCTGCCTGT TTACTGCCTT
    GCCCATCACC TCGAACTGTC TTACCAGGCG GTGTTCCAGA GCGTTCCCCT CTACAGCCGT
    GTGAAAGACC TTCTTCATAG CATGCACCGC TTCTATCACG ATTCTCCTGC TCACAGGAGT
    GCTCTGGTGA CTGCTTTCAA GGGCCTCCAC CTTCAACCTG CGATGCCCTC TCAAGTCAGA
    GGCCGGAGGT GGCTTCAGGG ATTGCAGGCT GCCCTCCAGA ACTTTCTCAA GGGATATCCA
    GCAATTGTCC AGCAGCTGCA GTCAACAAAA GAAAGCAGAA GCAACAGCAG TCATCAGAAA
    GCCGAAGAAC TCCTGGAGTC TCTCCTCCAG GCAGACATTG TCAAATTTGT CCACTTCTTG
    GTGGATGTCG TCAATGTCCT TAGCATACTG TCCCATGTCA ATCAGAACAG AAATTCCTCA
    ATTGCTGATG TATTTGCCAC CTTAGAGTCA ACACTGGAAA TGCTCCAAAT TTATCAGACA
    AGACCAGGGC CGAAAGAACG TGGGGTAGAT TCGGTCACAC ACTTTCATGG TAACTGCCTT
    GGAGGAAAGG GAAACATTTC TGACGTGAGA AACATTGTTT TAACACATCT TATCAAGAGG
    CTGCGAGGCT GTTTCAGAGA TGCCAACCAA GATGTGGTGA AGGCAACCAC GATTGGAAGC
    TTTAAACTGT GGCCGGCAAA AATAAATCAA GAATTTGGAG AAAAAGAGGT GGCCATCCTG
    ATTGCACATT ATGAACCAGT ATTAGAGGCA GCCAAGGTGA AAATTGACCA AGTAGACACA
    GAATGGAGCA TGTTGAAATT GGAGATCTAC TCTAGATTTC AGAACATTTG CAAACTGACC
    TGGGATTTTG TGAATTCTGT TTATTTACAC AAGTATCCAA ATATCCTGAC ACTCATTGAC
    CTCGTGCTGA GCCTGCCTGC AAGTTCAGCT GAGGCAGGAC GTGGCTTTAG TCAGATGAAG
    CGCATCAGGT CCCAAATGCA TGCCAAGGTC AAGGCTGACA ACATGACGGA TCTCCTGATA
    ATTCAGTTGA ATTCTCCAGA CATTAACAAC TTTGACCCTA GGAAAGCCAT TCATTTATGG
    AATACAAGAA CACCATCCTC AACTGAGGAC ACAAGACCTA ACTCAGACTG CTCATCAAAC
    TCAGAAAGCC TTAGTGAAAG CGATGAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OSm32431
    Clone ID Related Accession (Same CDS sequence) XM_012097085.2
    Accession Version XM_012097085.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5205bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-16
    Organism Ovis aries(sheep)
    Product sperm flagellar protein 2 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014639025.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012097085.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGTCGGAAA TCCTGTGCCA GTGGCTCAAC CAGGAATTGA AGGTGTCCCA GACCGTGAGT 
    CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAA TTGGAGAAGT TTTGCACAAG
    TTTGCACTTC AGGATGATTT TGCAGACTTT TCAGAAAGCA GGATTTCCAG TGCCAGACTT
    AACAATTTTT CTCGCTTGGA GCCCACACTT CACCTTTTGG GTGTGCAATT TGATCAGAAT
    GTGGCCCACA ATATCATCAC AGAGAAGCCT GGGGCTGCCA CGAAACTCTT ATATCAGTTG
    TACATTGCTC TCCAGAGGAA AAAGAAAGGT GTTCAGACTA GAGTGGAAAT GCCAACTGTA
    CAGCCCCTGA TGAGCACAAA AGTTCAAAAC ATGAAAAGCG AGGCTTTCCG AGATCAACGC
    TTGAGCAAAA GACGACAAAA TGAAATCATG GCCAAAATCC AAGCAGCTAT CATACAGATT
    CCTAAACCCA CATCAAATCG TACTCTGAAA ACATTTGACG CCCAAAAATT AATGAAAAAG
    AAAAAAGAGG CAGAGGATGT GGCCAATGAA ATTAAGAAGT TTGAAGCATT AATAAAAAAG
    GATCTCCAGG CAAAAGAAAG TGCATCCAAG ACTTCTCTAG ATACAGCAGG CCAGACAACC
    GCTGATTTGT TAAACACTTA TTCAGATGAT GACTACATTA AAAAAATCCA AAAACGCCTT
    GAAGAAGATG CTTTTGCACG AGAGCAAAGG GAGAAAAGAC GGCGGAGACT CTTAATGGAT
    CAGTTAATAG CTCATGAAGC CCAAGAGGAG GCTTATCGGG AGGAGCAGTT GATCAACCGG
    CTGATGCGAC AGTCCCAGCA GGAACGCAGG ATCGCTGTGC AGCTCATGCA CGTTAGGCAT
    GAAAAGGAAG TTTTATGGCA AAACAGAATT TTCAGAGAAA AACAATATGA GGAAAGACGG
    CTTAAAGACT TCCAGGATGC TCTTGATCGA GAAGCGGCTT TAGCCAGACA AGCCAAGATT
    GATTTTGAAG AACAAGCCCT CAGAGATAAG GAAATTCATG AGCAGATCGC CATAGAAAGA
    GCTCAAGCTC GTTACAAAAA GCATTATTCA ATATGTGCAG AAATTTTGGA TCAGATACTT
    GATCTGTCCA CTAAGGTGGC AGACTATCGA TTGTTGACAA ATAACCTGAT CCCATATAAG
    TTGATGCATG ATTGGAAGGA ACTGTTTTTC CATGGAAAAC CCATATATGA GCAAGCCTCT
    CTTAGACATT TACCAGCTAA GCTCTCTGCA GAACACCTTG TAGAAATGGA GAAAAGGAAC
    TTGCTAGATA ACAATGATTA TGAAGAATAT AAGAACATGG TTGGAGAATG GGCCTTACCA
    GAAGAAATGG TTGAAAACTT ACCACCCTCC AACAATTGCA TATTGGGCCA TGTGATTCAC
    AGACTAATTG AAAGTTCTCT TCCTCCTCAA GTTGAATCTA CAGTACCTGA GTTACCTTCA
    TTTGCTATAA AAGGATGCTT GTTGGGGAAA ACCTTTAGTG GAAAAACTAC TGCTCTAAAG
    GCTCTAGAGA AAAGCTTTCC TATTCAGGTA CTTTCTATTG ACACTCTTGT TCAAGAAGCT
    ATCCAAGCAT TTCATGACAA TGAAAGTATC AGCGGGGCCC ATGGACTTCA GAAAGCACCT
    GAAGAAAAGG TTTTACCAGT TAGGCGAAAG AGTGGTAAAG AAAACCAGGA CGTGCAAGAT
    GAAGCACTAC CAGAAAGAGA AGATAAAACC AGACTTGATA CCGATGCTAG AAAAGCAGCA
    AAAGCTGAAG AAGTCGTAGC GAGGGATAGT TTCTCTGAAC TCACTGTGCG TGCTCAGCTT
    GGTGCAAAAT CAGAAATGCT GCTGAAGAAA GGAAAGAGCA TATCTGATAT ATTGCTAGTC
    AGCATCATGG TAAATGCCAT TAATCAGATA CCTATGTATA AAGGCTGGAT CCTGGATGGC
    TTTCCAATGA CATTAAACCA AGCAAAACTT CTGGAGGAAG CACTCACAGG CTACAACAGA
    AACCTCATAG AACTGGAGGG AAAGAAATCA CAAACATCCA CATTAGCTGT TGACCCTACA
    GCTTCCAAAG AAGTGCCTCT TCCCCCTTCT GCATTTGATT TTGTCATGTT ATTAGACATT
    TCAGATAATT CCTCACTGAA TCGCATGAAT GACATCATGG CTAAAGCATT TTCAAGTGAA
    GCTCCTCGTG AAGATATCAA TCAGCGTGTA GCTGCTAAAA ACAAAGATAC AGATGAGGAC
    CAGAATTTAA GAGATCAAAT ACAGCACAGA ATTGTAGGCT TCCTGGACAA CTGGCCTTCG
    TTGGAGCAGT GGTTTACAGA GCCAGAAAAC ATTTTGATAA AAATCAATGC TGAAGTAGAT
    AAACAGTCAT TGTTCCAAAA AGTAAAGGAA ATGATTATGA CTGAAATAAT TAAAAAAGAA
    AATAAAGTGA AAAAGAAATT AGAAGAAAAG GAAGCTGAGA GAAAAGAAGA AGTTTCCCTG
    AGTGAGAAGA CATCCCCTCC TCCTCCTCCC CCTCCGGAAC CAGAGAAAGA GAAGGAAGTC
    CACCTTCCAC AGGAGCGGTC CAAAACTCCT GCTGCAAAAG GAAAGCAAGC ATCAGAACCC
    CTGCATGGTA ATCGAGAACC TCTTCAGGAA GGAAAAGGGA AGAAAAGTGA AACTTCACTC
    AAAAGAAAAG GTTCTCCTAG AGGAAAATCG CTAGGAGGGA AGATTCCACT CAAGAAATCA
    CCTGCCGAGT CTACAGATAT TTCACCGGTC CCGGTAGTGC CAGCCCCATC GAAGCCCGGA
    TCAGAAGAGT GGGTGTATGT AAATGAACCA ATTCCTGAGG AAATACCATC ATTTTTAGTA
    CCATACTGGA AATTGATAGA AAACTCCTAT ATAAATTCCA TCAAAACAGT ACTCAGGGAG
    TTGAGAGAAG GCCAGCATAG TGTACTTGCT TACTTATATG ATATTAGAAC AGGCTTCCAG
    CAGTTTCTGA AGCGTCCAGA CCACAAGCAA GACTTCGTGT CTCAGTGGCA GGCGGATTTC
    AACTCCCTTC CTGATGACCT GTGGGATGAT GAGGAAACAA AGGCTGAGCT GCACCAAAGA
    GTGAATGATT TACGGGACCG CCTGTGGGAC ATTTGCGATG CCCGTAAGGA AGAAGCAGAG
    CAGGAGCGTC TGGACATCAT TAACGAGAGC TGGTTGCAGG ACTCAATGGG AATTGCCATG
    AACCATTTCT TCTCACTGAT GCAGGCAGAA CTGAACCGTT TCCAAGATAC GAAGAGATTC
    CTGCAAGATT ATTACAGGGC CATGGAATGT AAAATTCCGA TGGATGACAC TAAGAAATTC
    ACTCGAGTCC CACTGGTCCG ACTGGATAGT AGAGATATTT TGGAAAGCCA GCTTAGAATT
    CCCCTGGTTC CTCGAAGATC CATTTCTCCA GAGTCAGCTT TATCCAAACC AAAAACAAAA
    AGCATCCTGA AGGGCAAGAT CGATTACACG CTGGAAAGTG TAGAGTTAAA CTTTGAGACT
    GATGAGAAGA TGGTGATGGA TACGTGGCAG CAGTCTTCTT TGGCAGTATC TCACATGGTG
    GCTGCTGAAA TTCATCAGAG GCTCATGGAA GAAGAAAAAG AAAACCAGCC AGCAGAAACA
    AAAGAAAAAC TTCCTCAGGC AGCTGCAAAT AAAAAAGTGA AAAAGGAGCC TCCGCCACCG
    CCACCACCAC CCAAGAAAAA GAAAGAGGAC AAAAAAGGAA AAGGTAAATC CCCACCTATG
    GCAGAAGCTA CTCCTGTAGT AGTGCCAGCA GAAGAGACTG AGGAAGTGGA ATTAAAAAAT
    GAACTGAAGC TCAGAATAAA GGAAGAGCAT CTTGCTGCCC TGCAATTTGA AGAGATAGCT
    ACACAGTTCC GACTTGAACT GATAAAGGTA AAAGCATTGG CTTTCCTGGA AGAGTTAGTA
    ATAAAGGCGG TTGATGTATA TAGACTCATG GACAAGTGGC TCGGTGAGAG GTACTTGAAT
    GAAATGGCCA GTGTGGAAAA ACTAACTGGG GTAGCTCGCT ATCACATTGA AACATCAACC
    AAAATTCAGA ATGAACTTTA TTTAGACCAA GAAGACTTCT TCATTAATGG CGATATCAAA
    GTCTTTCCAG ATCCACCTCC TCCGGTACGC CCTCCGCCTG TGGAAAAGGA GGAAAATGGC
    ACCCTGACCA TGGAGCAGCT TGACAACCTC CGGGATCAGT TCTTAGATAT AGCGCCCAAA
    GGTATAATAG GAAATAAAGC ATTTGCTGAC ATTCTGCTGG ATTTGGTAAC TCTGAACCTT
    GGAACAAACA ACTTTCCTAC TAGTTGGATG AACCTCACTC AACCGGAATT GCAGGAGTTA
    GCATCTTTGT TGGTAACAAA CTCGGAGTCC GTGGACTGGC GGAAATTCTT GTTAGTAGTA
    GCGCTGCCTT GGCCCATCCC CCTGGAGGAG GAGCTTCTGG AGACCCTGCA GAGGTTCAAG
    GCCCTGGATG CGGAGCAGCT GGGCACCGTC ACTTATGAAC AGTATAAGCA GGCTGGCCTG
    TGGTTTACAG GAGATGAAGA TATCAAAATT CCAGAAAATC CTCTTGAACC CCTGCCATTC
    AATAGGCAGG AGCATCTTAT AGAGTTTTTC TTTAGGTTGT TTGCTGACTG CGAGAAGGTT
    CCACCCCAGC TTGACTACAC ACAGATGCTG CTTTATTTTG CTTGCCATCC AGACAGCACA
    GAAGGGGTCT ACAGGGCCCT CAGCGTAGCC ATTGGGACTC ACGTCTTCCA GCCAATTGAA
    ATGCCTCATC CTGTTGCAGC AAAGACCTCT TCCTTTACTG ATATGAGTCC CATTGAGGAA
    TATCCTGAAC TCGAGGACAA CTTTATGAGT GAGGAAAGGG AATTACAAGA GGACGGGGAG
    GAAAAGGAAG AAGAAATCCC TGAAAATGCA AATACGGAAA TGATTTCCAT GCAAACATTA
    CTCCAAGTGT TCCGAGGAGG AAATGAAGTA CTGGACGCTA ACAGATTTGC CAGCCACCAA
    AAGATAGAGA ACATTTATTT AGAGAACTTC ATAAAAGTGT TTCAGGACCT AGGTTCCAAG
    AACCTTGAGC CAATTGAAGT TGCTGTTCTT CTGAAGCATC CTTTTATTCA AGACCTGATT
    TCAAGTTATC CAGACTATAA GATCCCTATA TCCATGTGCA AGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011952475.1
    CDS493..5697
    Translation

    Target ORF information:

    RefSeq Version XM_012097085.2
    Organism Ovis aries(sheep)
    Definition Ovis aries sperm flagellar 2 (SPEF2), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012097085.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGTCGGAAA TCCTGTGCCA GTGGCTCAAC CAGGAATTGA AGGTGTCCCA GACCGTGAGT 
    CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAA TTGGAGAAGT TTTGCACAAG
    TTTGCACTTC AGGATGATTT TGCAGACTTT TCAGAAAGCA GGATTTCCAG TGCCAGACTT
    AACAATTTTT CTCGCTTGGA GCCCACACTT CACCTTTTGG GTGTGCAATT TGATCAGAAT
    GTGGCCCACA ATATCATCAC AGAGAAGCCT GGGGCTGCCA CGAAACTCTT ATATCAGTTG
    TACATTGCTC TCCAGAGGAA AAAGAAAGGT GTTCAGACTA GAGTGGAAAT GCCAACTGTA
    CAGCCCCTGA TGAGCACAAA AGTTCAAAAC ATGAAAAGCG AGGCTTTCCG AGATCAACGC
    TTGAGCAAAA GACGACAAAA TGAAATCATG GCCAAAATCC AAGCAGCTAT CATACAGATT
    CCTAAACCCA CATCAAATCG TACTCTGAAA ACATTTGACG CCCAAAAATT AATGAAAAAG
    AAAAAAGAGG CAGAGGATGT GGCCAATGAA ATTAAGAAGT TTGAAGCATT AATAAAAAAG
    GATCTCCAGG CAAAAGAAAG TGCATCCAAG ACTTCTCTAG ATACAGCAGG CCAGACAACC
    GCTGATTTGT TAAACACTTA TTCAGATGAT GACTACATTA AAAAAATCCA AAAACGCCTT
    GAAGAAGATG CTTTTGCACG AGAGCAAAGG GAGAAAAGAC GGCGGAGACT CTTAATGGAT
    CAGTTAATAG CTCATGAAGC CCAAGAGGAG GCTTATCGGG AGGAGCAGTT GATCAACCGG
    CTGATGCGAC AGTCCCAGCA GGAACGCAGG ATCGCTGTGC AGCTCATGCA CGTTAGGCAT
    GAAAAGGAAG TTTTATGGCA AAACAGAATT TTCAGAGAAA AACAATATGA GGAAAGACGG
    CTTAAAGACT TCCAGGATGC TCTTGATCGA GAAGCGGCTT TAGCCAGACA AGCCAAGATT
    GATTTTGAAG AACAAGCCCT CAGAGATAAG GAAATTCATG AGCAGATCGC CATAGAAAGA
    GCTCAAGCTC GTTACAAAAA GCATTATTCA ATATGTGCAG AAATTTTGGA TCAGATACTT
    GATCTGTCCA CTAAGGTGGC AGACTATCGA TTGTTGACAA ATAACCTGAT CCCATATAAG
    TTGATGCATG ATTGGAAGGA ACTGTTTTTC CATGGAAAAC CCATATATGA GCAAGCCTCT
    CTTAGACATT TACCAGCTAA GCTCTCTGCA GAACACCTTG TAGAAATGGA GAAAAGGAAC
    TTGCTAGATA ACAATGATTA TGAAGAATAT AAGAACATGG TTGGAGAATG GGCCTTACCA
    GAAGAAATGG TTGAAAACTT ACCACCCTCC AACAATTGCA TATTGGGCCA TGTGATTCAC
    AGACTAATTG AAAGTTCTCT TCCTCCTCAA GTTGAATCTA CAGTACCTGA GTTACCTTCA
    TTTGCTATAA AAGGATGCTT GTTGGGGAAA ACCTTTAGTG GAAAAACTAC TGCTCTAAAG
    GCTCTAGAGA AAAGCTTTCC TATTCAGGTA CTTTCTATTG ACACTCTTGT TCAAGAAGCT
    ATCCAAGCAT TTCATGACAA TGAAAGTATC AGCGGGGCCC ATGGACTTCA GAAAGCACCT
    GAAGAAAAGG TTTTACCAGT TAGGCGAAAG AGTGGTAAAG AAAACCAGGA CGTGCAAGAT
    GAAGCACTAC CAGAAAGAGA AGATAAAACC AGACTTGATA CCGATGCTAG AAAAGCAGCA
    AAAGCTGAAG AAGTCGTAGC GAGGGATAGT TTCTCTGAAC TCACTGTGCG TGCTCAGCTT
    GGTGCAAAAT CAGAAATGCT GCTGAAGAAA GGAAAGAGCA TATCTGATAT ATTGCTAGTC
    AGCATCATGG TAAATGCCAT TAATCAGATA CCTATGTATA AAGGCTGGAT CCTGGATGGC
    TTTCCAATGA CATTAAACCA AGCAAAACTT CTGGAGGAAG CACTCACAGG CTACAACAGA
    AACCTCATAG AACTGGAGGG AAAGAAATCA CAAACATCCA CATTAGCTGT TGACCCTACA
    GCTTCCAAAG AAGTGCCTCT TCCCCCTTCT GCATTTGATT TTGTCATGTT ATTAGACATT
    TCAGATAATT CCTCACTGAA TCGCATGAAT GACATCATGG CTAAAGCATT TTCAAGTGAA
    GCTCCTCGTG AAGATATCAA TCAGCGTGTA GCTGCTAAAA ACAAAGATAC AGATGAGGAC
    CAGAATTTAA GAGATCAAAT ACAGCACAGA ATTGTAGGCT TCCTGGACAA CTGGCCTTCG
    TTGGAGCAGT GGTTTACAGA GCCAGAAAAC ATTTTGATAA AAATCAATGC TGAAGTAGAT
    AAACAGTCAT TGTTCCAAAA AGTAAAGGAA ATGATTATGA CTGAAATAAT TAAAAAAGAA
    AATAAAGTGA AAAAGAAATT AGAAGAAAAG GAAGCTGAGA GAAAAGAAGA AGTTTCCCTG
    AGTGAGAAGA CATCCCCTCC TCCTCCTCCC CCTCCGGAAC CAGAGAAAGA GAAGGAAGTC
    CACCTTCCAC AGGAGCGGTC CAAAACTCCT GCTGCAAAAG GAAAGCAAGC ATCAGAACCC
    CTGCATGGTA ATCGAGAACC TCTTCAGGAA GGAAAAGGGA AGAAAAGTGA AACTTCACTC
    AAAAGAAAAG GTTCTCCTAG AGGAAAATCG CTAGGAGGGA AGATTCCACT CAAGAAATCA
    CCTGCCGAGT CTACAGATAT TTCACCGGTC CCGGTAGTGC CAGCCCCATC GAAGCCCGGA
    TCAGAAGAGT GGGTGTATGT AAATGAACCA ATTCCTGAGG AAATACCATC ATTTTTAGTA
    CCATACTGGA AATTGATAGA AAACTCCTAT ATAAATTCCA TCAAAACAGT ACTCAGGGAG
    TTGAGAGAAG GCCAGCATAG TGTACTTGCT TACTTATATG ATATTAGAAC AGGCTTCCAG
    CAGTTTCTGA AGCGTCCAGA CCACAAGCAA GACTTCGTGT CTCAGTGGCA GGCGGATTTC
    AACTCCCTTC CTGATGACCT GTGGGATGAT GAGGAAACAA AGGCTGAGCT GCACCAAAGA
    GTGAATGATT TACGGGACCG CCTGTGGGAC ATTTGCGATG CCCGTAAGGA AGAAGCAGAG
    CAGGAGCGTC TGGACATCAT TAACGAGAGC TGGTTGCAGG ACTCAATGGG AATTGCCATG
    AACCATTTCT TCTCACTGAT GCAGGCAGAA CTGAACCGTT TCCAAGATAC GAAGAGATTC
    CTGCAAGATT ATTACAGGGC CATGGAATGT AAAATTCCGA TGGATGACAC TAAGAAATTC
    ACTCGAGTCC CACTGGTCCG ACTGGATAGT AGAGATATTT TGGAAAGCCA GCTTAGAATT
    CCCCTGGTTC CTCGAAGATC CATTTCTCCA GAGTCAGCTT TATCCAAACC AAAAACAAAA
    AGCATCCTGA AGGGCAAGAT CGATTACACG CTGGAAAGTG TAGAGTTAAA CTTTGAGACT
    GATGAGAAGA TGGTGATGGA TACGTGGCAG CAGTCTTCTT TGGCAGTATC TCACATGGTG
    GCTGCTGAAA TTCATCAGAG GCTCATGGAA GAAGAAAAAG AAAACCAGCC AGCAGAAACA
    AAAGAAAAAC TTCCTCAGGC AGCTGCAAAT AAAAAAGTGA AAAAGGAGCC TCCGCCACCG
    CCACCACCAC CCAAGAAAAA GAAAGAGGAC AAAAAAGGAA AAGGTAAATC CCCACCTATG
    GCAGAAGCTA CTCCTGTAGT AGTGCCAGCA GAAGAGACTG AGGAAGTGGA ATTAAAAAAT
    GAACTGAAGC TCAGAATAAA GGAAGAGCAT CTTGCTGCCC TGCAATTTGA AGAGATAGCT
    ACACAGTTCC GACTTGAACT GATAAAGGTA AAAGCATTGG CTTTCCTGGA AGAGTTAGTA
    ATAAAGGCGG TTGATGTATA TAGACTCATG GACAAGTGGC TCGGTGAGAG GTACTTGAAT
    GAAATGGCCA GTGTGGAAAA ACTAACTGGG GTAGCTCGCT ATCACATTGA AACATCAACC
    AAAATTCAGA ATGAACTTTA TTTAGACCAA GAAGACTTCT TCATTAATGG CGATATCAAA
    GTCTTTCCAG ATCCACCTCC TCCGGTACGC CCTCCGCCTG TGGAAAAGGA GGAAAATGGC
    ACCCTGACCA TGGAGCAGCT TGACAACCTC CGGGATCAGT TCTTAGATAT AGCGCCCAAA
    GGTATAATAG GAAATAAAGC ATTTGCTGAC ATTCTGCTGG ATTTGGTAAC TCTGAACCTT
    GGAACAAACA ACTTTCCTAC TAGTTGGATG AACCTCACTC AACCGGAATT GCAGGAGTTA
    GCATCTTTGT TGGTAACAAA CTCGGAGTCC GTGGACTGGC GGAAATTCTT GTTAGTAGTA
    GCGCTGCCTT GGCCCATCCC CCTGGAGGAG GAGCTTCTGG AGACCCTGCA GAGGTTCAAG
    GCCCTGGATG CGGAGCAGCT GGGCACCGTC ACTTATGAAC AGTATAAGCA GGCTGGCCTG
    TGGTTTACAG GAGATGAAGA TATCAAAATT CCAGAAAATC CTCTTGAACC CCTGCCATTC
    AATAGGCAGG AGCATCTTAT AGAGTTTTTC TTTAGGTTGT TTGCTGACTG CGAGAAGGTT
    CCACCCCAGC TTGACTACAC ACAGATGCTG CTTTATTTTG CTTGCCATCC AGACAGCACA
    GAAGGGGTCT ACAGGGCCCT CAGCGTAGCC ATTGGGACTC ACGTCTTCCA GCCAATTGAA
    ATGCCTCATC CTGTTGCAGC AAAGACCTCT TCCTTTACTG ATATGAGTCC CATTGAGGAA
    TATCCTGAAC TCGAGGACAA CTTTATGAGT GAGGAAAGGG AATTACAAGA GGACGGGGAG
    GAAAAGGAAG AAGAAATCCC TGAAAATGCA AATACGGAAA TGATTTCCAT GCAAACATTA
    CTCCAAGTGT TCCGAGGAGG AAATGAAGTA CTGGACGCTA ACAGATTTGC CAGCCACCAA
    AAGATAGAGA ACATTTATTT AGAGAACTTC ATAAAAGTGT TTCAGGACCT AGGTTCCAAG
    AACCTTGAGC CAATTGAAGT TGCTGTTCTT CTGAAGCATC CTTTTATTCA AGACCTGATT
    TCAAGTTATC CAGACTATAA GATCCCTATA TCCATGTGCA AGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OSm32432
    Clone ID Related Accession (Same CDS sequence) XM_012097086.2
    Accession Version XM_012097086.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5133bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-16
    Organism Ovis aries(sheep)
    Product sperm flagellar protein 2 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014639025.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012097086.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGCATGATT GGAAGGAACT GTTTTTCCAT GGAAAACCCA TATATGAGCA AGCCTCTCTT 
    AGACATTTAC CAGCTAAGCT CTCTGCAGAA CACCTTGTAG AAATGGAGAA AAGGAACTTG
    CTAGATAACA ATGATTATGA AGAATATAAG AACATGGTTG GAGAATGGGC CTTACCAGAA
    GAAATGGTTG AAAACTTACC ACCCTCCAAC AATTGCATAT TGGGCCATGT GATTCACAGA
    CTAATTGAAA GTTCTCTTCC TCCTCAAGTT GAATCTACAG TACCTGAGTT ACCTTCATTT
    GCTATAAAAG GATGCTTGTT GGGGAAAACC TTTAGTGGAA AAACTACTGC TCTAAAGGCT
    CTAGAGAAAA GCTTTCCTAT TCAGGTACTT TCTATTGACA CTCTTGTTCA AGAAGCTATC
    CAAGCATTTC ATGACAATGA AAGTATCAGC GGGGCCCATG GACTTCAGAA AGCACCTGAA
    GAAAAGGTTT TACCAGTTAG GCGAAAGAGT GGTAAAGAAA ACCAGGACGT GCAAGATGAA
    GCACTACCAG AAAGAGAAGA TAAAACCAGA CTTGATACCG ATGCTAGAAA AGCAGCAAAA
    GCTGAAGAAG TCGTAGCGAG GGATAGTTTC TCTGAACTCA CTGTGCGTGC TCAGCTTGGT
    GCAAAATCAG AAATGCTGCT GAAGAAAGGA AAGAGCATAT CTGATATATT GCTAGTCAGC
    ATCATGGTAA ATGCCATTAA TCAGATACCT ATGTATAAAG GCTGGATCCT GGATGGCTTT
    CCAATGACAT TAAACCAAGC AAAACTTCTG GAGGAAGCAC TCACAGGCTA CAACAGAAAC
    CTCATAGAAC TGGAGGGAAA GAAATCACAA ACATCCACAT TAGCTGTTGA CCCTACAGCT
    TCCAAAGAAG TGCCTCTTCC CCCTTCTGCA TTTGATTTTG TCATGTTATT AGACATTTCA
    GATAATTCCT CACTGAATCG CATGAATGAC ATCATGGCTA AAGCATTTTC AAGTGAAGCT
    CCTCGTGAAG ATATCAATCA GCGTGTAGCT GCTAAAAACA AAGATACAGA TGAGGACCAG
    AATTTAAGAG ATCAAATACA GCACAGAATT GTAGGCTTCC TGGACAACTG GCCTTCGTTG
    GAGCAGTGGT TTACAGAGCC AGAAAACATT TTGATAAAAA TCAATGCTGA AGTAGATAAA
    CAGTCATTGT TCCAAAAAGT AAAGGAAATG ATTATGACTG AAATAATTAA AAAAGAAAAT
    AAAGTGAAAA AGAAATTAGA AGAAAAGGAA GCTGAGAGAA AAGAAGAAGT TTCCCTGAGT
    GAGAAGACAT CCCCTCCTCC TCCTCCCCCT CCGGAACCAG AGAAAGAGAA GGAAGTCCAC
    CTTCCACAGG AGCGGTCCAA AACTCCTGCT GCAAAAGGAA AGCAAGCATC AGAACCCCTG
    CATGGTAATC GAGAACCTCT TCAGGAAGGA AAAGGGAAGA AAAGTGAAAC TTCACTCAAA
    AGAAAAGGTT CTCCTAGAGG AAAATCGCTA GGAGGGAAGA TTCCACTCAA GAAATCACCT
    GCCGAGTCTA CAGATATTTC ACCGGTCCCG GTAGTGCCAG CCCCATCGAA GCCCGGATCA
    GAAGAGTGGG TGTATGTAAA TGAACCAATT CCTGAGGAAA TACCATCATT TTTAGTACCA
    TACTGGAAAT TGATAGAAAA CTCCTATATA AATTCCATCA AAACAGTACT CAGGGAGTTG
    AGAGAAGGCC AGCATAGTGT ACTTGCTTAC TTATATGATA TTAGAACAGG CTTCCAGCAG
    TTTCTGAAGC GTCCAGACCA CAAGCAAGAC TTCGTGTCTC AGTGGCAGGC GGATTTCAAC
    TCCCTTCCTG ATGACCTGTG GGATGATGAG GAAACAAAGG CTGAGCTGCA CCAAAGAGTG
    AATGATTTAC GGGACCGCCT GTGGGACATT TGCGATGCCC GTAAGGAAGA AGCAGAGCAG
    GAGCGTCTGG ACATCATTAA CGAGAGCTGG TTGCAGGACT CAATGGGAAT TGCCATGAAC
    CATTTCTTCT CACTGATGCA GGCAGAACTG AACCGTTTCC AAGATACGAA GAGATTCCTG
    CAAGATTATT ACAGGGCCAT GGAATGTAAA ATTCCGATGG ATGACACTAA GAAATTCACT
    CGAGTCCCAC TGGTCCGACT GGATAGTAGA GATATTTTGG AAAGCCAGCT TAGAATTCCC
    CTGGTTCCTC GAAGATCCAT TTCTCCAGAG TCAGCTTTAT CCAAACCAAA AACAAAAAGC
    ATCCTGAAGG GCAAGATCGA TTACACGCTG GAAAGTGTAG AGTTAAACTT TGAGACTGAT
    GAGAAGATGG TGATGGATAC GTGGCAGCAG TCTTCTTTGG CAGTATCTCA CATGGTGGCT
    GCTGAAATTC ATCAGAGGCT CATGGAAGAA GAAAAAGAAA ACCAGCCAGC AGAAACAAAA
    GAAAAACTTC CTCAGGCAGC TGCAAATAAA AAAGTGAAAA AGGAGCCTCC GCCACCGCCA
    CCACCACCCA AGAAAAAGAA AGAGGACAAA AAAGGAAAAG GTAAATCCCC ACCTATGGCA
    GAAGCTACTC CTGTAGTAGT GCCAGCAGAA GAGACTGAGG AAGTGGAATT AAAAAATGAA
    CTGAAGCTCA GAATAAAGGA AGAGCATCTT GCTGCCCTGC AATTTGAAGA GATAGCTACA
    CAGTTCCGAC TTGAACTGAT AAAGGTAAAA GCATTGGCTT TCCTGGAAGA GTTAGTAATA
    AAGGCGGTTG ATGTATATAG ACTCATGGAC AAGTGGCTCG GTGAGAGGTA CTTGAATGAA
    ATGGCCAGTG TGGAAAAACT AACTGGGGTA GCTCGCTATC ACATTGAAAC ATCAACCAAA
    ATTCAGAATG AACTTTATTT AGACCAAGAA GACTTCTTCA TTAATGGCGA TATCAAAGTC
    TTTCCAGATC CACCTCCTCC GGTACGCCCT CCGCCTGTGG AAAAGGAGGA AAATGGCACC
    CTGACCATGG AGCAGCTTGA CAACCTCCGG GATCAGTTCT TAGATATAGC GCCCAAAGGC
    AGGGATCCTC CAGACAATGT GAGTGGATTC ACCAAGAAGC AGAAAAGGCC TCAGAAGCTT
    GACCATTACC TCCCAAAGAG GAACAATCAA TTTTTGAAAA TTTGGCTTAA AAAATACCCT
    TGGCTTGTAT ACGATGAGAT ACTAAATCTT ATGTTCTGCG GCCTGTGTCG TAAACATGGA
    GTGACATCAA AGGGAAATCA GGTCAGTTTT TTTTACGGCA CGGATAACTT CAGGACTGAA
    TTTCTCAACG CGCATCATCT GAGTGAGGCT CACGCCAAGG CGTCCCTCAT GGAAGCCACA
    AGTGGTTCTC CAGGGAGCAG AGCTACCACG GAGCTGATGG TGAGGACCAT GAGCAAGGTG
    ACCCTTGGCA GGGTGGAGAA CCTGTTCCGG TCCTGCCACG CCATCGCCAA GACCGGACGT
    CCCCTCAAAG ACTTCCTCTG GATGTGTTCG CTGGACGATA TGAAGGGAAT TGATATCGGC
    CCGGTGTTCA GAACCAGTAA ATCGGCCAGA ACTTTTACCT ACTTTATTGC TGAAGTGGAA
    CGGAGAACTC TAAGAGAGAA GTTAGAAAAA AGTCAGTTCT TCTCCGTCCT CAGTGATGGG
    ATTGCAGACA ATTCAGCTGA GGAAGCTGAA CTCGTATATG TGCAGTTTGC CCGCGCAGGG
    AGAGTGCACT GCCAAATCGT CGGGGTACAG ACCGTGGAGA AGAAGGAGCC CTGGGCGATA
    AAACGTGCCA TTGAGAAAAC TCTGGAGAGA AATCTTCAAC TTCGACTGTC GAACCAAGAC
    TGGGCAAAGA AACTGGTGGG TTTCGGAAGC GATGGAGCTC CAGGTTCAGA GGGAGAAAAC
    GGCAGCACAG TGGCCCTGCT TTTAAGGGAA ATCCAGCCAG GTGTGCTGCC TGTTTACTGC
    CTTGCCCATC ACCTCGAACT GTCTTACCAG GCGGTGTTCC AGAGCGTTCC CCTCTACAGC
    CGTGTGAAAG ACCTTCTTCA TAGCATGCAC CGCTTCTATC ACGATTCTCC TGCTCACAGG
    AGTGCTCTGG TGACTGCTTT CAAGGGCCTC CACCTTCAAC CTGCGATGCC CTCTCAAGTC
    AGAGGCCGGA GGTGGCTTCA GGGATTGCAG GCTGCCCTCC AGAACTTTCT CAAGGGATAT
    CCAGCAATTG TCCAGCAGCT GCAGTCAACA AAAGAAAGCA GAAGCAACAG CAGTCATCAG
    AAAGCCGAAG AACTCCTGGA GTCTCTCCTC CAGGCAGACA TTGTCAAATT TGTCCACTTC
    TTGGTGGATG TCGTCAATGT CCTTAGCATA CTGTCCCATG TCAATCAGAA CAGAAATTCC
    TCAATTGCTG ATGTATTTGC CACCTTAGAG TCAACACTGG AAATGCTCCA AATTTATCAG
    ACAAGACCAG GGCCGAAAGA ACGTGGGGTA GATTCGGTCA CACACTTTCA TGGTAACTGC
    CTTGGAGGAA AGGGAAACAT TTCTGACGTG AGAAACATTG TTTTAACACA TCTTATCAAG
    AGGCTGCGAG GCTGTTTCAG AGATGCCAAC CAAGATGTGG TGAAGGCAAC CACGATTGGA
    AGCTTTAAAC TGTGGCCGGC AAAAATAAAT CAAGAATTTG GAGAAAAAGA GGTGGCCATC
    CTGATTGCAC ATTATGAACC AGTATTAGAG GCAGCCAAGG TGAAAATTGA CCAAGTAGAC
    ACAGAATGGA GCATGTTGAA ATTGGAGATC TACTCTAGAT TTCAGAACAT TTGCAAACTG
    ACCTGGGATT TTGTGAATTC TGTTTATTTA CACAAGTATC CAAATATCCT GACACTCATT
    GACCTCGTGC TGAGCCTGCC TGCAAGTTCA GCTGAGGCAG GACGTGGCTT TAGTCAGATG
    AAGCGCATCA GGTCCCAAAT GCATGCCAAG GTCAAGGCTG ACAACATGAC GGATCTCCTG
    ATAATTCAGT TGAATTCTCC AGACATTAAC AACTTTGACC CTAGGAAAGC CATTCATTTA
    TGGAATACAA GAACACCATC CTCAACTGAG GACACAAGAC CTAACTCAGA CTGCTCATCA
    AACTCAGAAA GCCTTAGTGA AAGCGATGAG TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011952476.1
    CDS1341..6473
    Translation

    Target ORF information:

    RefSeq Version XM_012097086.2
    Organism Ovis aries(sheep)
    Definition Ovis aries sperm flagellar 2 (SPEF2), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012097086.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGCATGATT GGAAGGAACT GTTTTTCCAT GGAAAACCCA TATATGAGCA AGCCTCTCTT 
    AGACATTTAC CAGCTAAGCT CTCTGCAGAA CACCTTGTAG AAATGGAGAA AAGGAACTTG
    CTAGATAACA ATGATTATGA AGAATATAAG AACATGGTTG GAGAATGGGC CTTACCAGAA
    GAAATGGTTG AAAACTTACC ACCCTCCAAC AATTGCATAT TGGGCCATGT GATTCACAGA
    CTAATTGAAA GTTCTCTTCC TCCTCAAGTT GAATCTACAG TACCTGAGTT ACCTTCATTT
    GCTATAAAAG GATGCTTGTT GGGGAAAACC TTTAGTGGAA AAACTACTGC TCTAAAGGCT
    CTAGAGAAAA GCTTTCCTAT TCAGGTACTT TCTATTGACA CTCTTGTTCA AGAAGCTATC
    CAAGCATTTC ATGACAATGA AAGTATCAGC GGGGCCCATG GACTTCAGAA AGCACCTGAA
    GAAAAGGTTT TACCAGTTAG GCGAAAGAGT GGTAAAGAAA ACCAGGACGT GCAAGATGAA
    GCACTACCAG AAAGAGAAGA TAAAACCAGA CTTGATACCG ATGCTAGAAA AGCAGCAAAA
    GCTGAAGAAG TCGTAGCGAG GGATAGTTTC TCTGAACTCA CTGTGCGTGC TCAGCTTGGT
    GCAAAATCAG AAATGCTGCT GAAGAAAGGA AAGAGCATAT CTGATATATT GCTAGTCAGC
    ATCATGGTAA ATGCCATTAA TCAGATACCT ATGTATAAAG GCTGGATCCT GGATGGCTTT
    CCAATGACAT TAAACCAAGC AAAACTTCTG GAGGAAGCAC TCACAGGCTA CAACAGAAAC
    CTCATAGAAC TGGAGGGAAA GAAATCACAA ACATCCACAT TAGCTGTTGA CCCTACAGCT
    TCCAAAGAAG TGCCTCTTCC CCCTTCTGCA TTTGATTTTG TCATGTTATT AGACATTTCA
    GATAATTCCT CACTGAATCG CATGAATGAC ATCATGGCTA AAGCATTTTC AAGTGAAGCT
    CCTCGTGAAG ATATCAATCA GCGTGTAGCT GCTAAAAACA AAGATACAGA TGAGGACCAG
    AATTTAAGAG ATCAAATACA GCACAGAATT GTAGGCTTCC TGGACAACTG GCCTTCGTTG
    GAGCAGTGGT TTACAGAGCC AGAAAACATT TTGATAAAAA TCAATGCTGA AGTAGATAAA
    CAGTCATTGT TCCAAAAAGT AAAGGAAATG ATTATGACTG AAATAATTAA AAAAGAAAAT
    AAAGTGAAAA AGAAATTAGA AGAAAAGGAA GCTGAGAGAA AAGAAGAAGT TTCCCTGAGT
    GAGAAGACAT CCCCTCCTCC TCCTCCCCCT CCGGAACCAG AGAAAGAGAA GGAAGTCCAC
    CTTCCACAGG AGCGGTCCAA AACTCCTGCT GCAAAAGGAA AGCAAGCATC AGAACCCCTG
    CATGGTAATC GAGAACCTCT TCAGGAAGGA AAAGGGAAGA AAAGTGAAAC TTCACTCAAA
    AGAAAAGGTT CTCCTAGAGG AAAATCGCTA GGAGGGAAGA TTCCACTCAA GAAATCACCT
    GCCGAGTCTA CAGATATTTC ACCGGTCCCG GTAGTGCCAG CCCCATCGAA GCCCGGATCA
    GAAGAGTGGG TGTATGTAAA TGAACCAATT CCTGAGGAAA TACCATCATT TTTAGTACCA
    TACTGGAAAT TGATAGAAAA CTCCTATATA AATTCCATCA AAACAGTACT CAGGGAGTTG
    AGAGAAGGCC AGCATAGTGT ACTTGCTTAC TTATATGATA TTAGAACAGG CTTCCAGCAG
    TTTCTGAAGC GTCCAGACCA CAAGCAAGAC TTCGTGTCTC AGTGGCAGGC GGATTTCAAC
    TCCCTTCCTG ATGACCTGTG GGATGATGAG GAAACAAAGG CTGAGCTGCA CCAAAGAGTG
    AATGATTTAC GGGACCGCCT GTGGGACATT TGCGATGCCC GTAAGGAAGA AGCAGAGCAG
    GAGCGTCTGG ACATCATTAA CGAGAGCTGG TTGCAGGACT CAATGGGAAT TGCCATGAAC
    CATTTCTTCT CACTGATGCA GGCAGAACTG AACCGTTTCC AAGATACGAA GAGATTCCTG
    CAAGATTATT ACAGGGCCAT GGAATGTAAA ATTCCGATGG ATGACACTAA GAAATTCACT
    CGAGTCCCAC TGGTCCGACT GGATAGTAGA GATATTTTGG AAAGCCAGCT TAGAATTCCC
    CTGGTTCCTC GAAGATCCAT TTCTCCAGAG TCAGCTTTAT CCAAACCAAA AACAAAAAGC
    ATCCTGAAGG GCAAGATCGA TTACACGCTG GAAAGTGTAG AGTTAAACTT TGAGACTGAT
    GAGAAGATGG TGATGGATAC GTGGCAGCAG TCTTCTTTGG CAGTATCTCA CATGGTGGCT
    GCTGAAATTC ATCAGAGGCT CATGGAAGAA GAAAAAGAAA ACCAGCCAGC AGAAACAAAA
    GAAAAACTTC CTCAGGCAGC TGCAAATAAA AAAGTGAAAA AGGAGCCTCC GCCACCGCCA
    CCACCACCCA AGAAAAAGAA AGAGGACAAA AAAGGAAAAG GTAAATCCCC ACCTATGGCA
    GAAGCTACTC CTGTAGTAGT GCCAGCAGAA GAGACTGAGG AAGTGGAATT AAAAAATGAA
    CTGAAGCTCA GAATAAAGGA AGAGCATCTT GCTGCCCTGC AATTTGAAGA GATAGCTACA
    CAGTTCCGAC TTGAACTGAT AAAGGTAAAA GCATTGGCTT TCCTGGAAGA GTTAGTAATA
    AAGGCGGTTG ATGTATATAG ACTCATGGAC AAGTGGCTCG GTGAGAGGTA CTTGAATGAA
    ATGGCCAGTG TGGAAAAACT AACTGGGGTA GCTCGCTATC ACATTGAAAC ATCAACCAAA
    ATTCAGAATG AACTTTATTT AGACCAAGAA GACTTCTTCA TTAATGGCGA TATCAAAGTC
    TTTCCAGATC CACCTCCTCC GGTACGCCCT CCGCCTGTGG AAAAGGAGGA AAATGGCACC
    CTGACCATGG AGCAGCTTGA CAACCTCCGG GATCAGTTCT TAGATATAGC GCCCAAAGGC
    AGGGATCCTC CAGACAATGT GAGTGGATTC ACCAAGAAGC AGAAAAGGCC TCAGAAGCTT
    GACCATTACC TCCCAAAGAG GAACAATCAA TTTTTGAAAA TTTGGCTTAA AAAATACCCT
    TGGCTTGTAT ACGATGAGAT ACTAAATCTT ATGTTCTGCG GCCTGTGTCG TAAACATGGA
    GTGACATCAA AGGGAAATCA GGTCAGTTTT TTTTACGGCA CGGATAACTT CAGGACTGAA
    TTTCTCAACG CGCATCATCT GAGTGAGGCT CACGCCAAGG CGTCCCTCAT GGAAGCCACA
    AGTGGTTCTC CAGGGAGCAG AGCTACCACG GAGCTGATGG TGAGGACCAT GAGCAAGGTG
    ACCCTTGGCA GGGTGGAGAA CCTGTTCCGG TCCTGCCACG CCATCGCCAA GACCGGACGT
    CCCCTCAAAG ACTTCCTCTG GATGTGTTCG CTGGACGATA TGAAGGGAAT TGATATCGGC
    CCGGTGTTCA GAACCAGTAA ATCGGCCAGA ACTTTTACCT ACTTTATTGC TGAAGTGGAA
    CGGAGAACTC TAAGAGAGAA GTTAGAAAAA AGTCAGTTCT TCTCCGTCCT CAGTGATGGG
    ATTGCAGACA ATTCAGCTGA GGAAGCTGAA CTCGTATATG TGCAGTTTGC CCGCGCAGGG
    AGAGTGCACT GCCAAATCGT CGGGGTACAG ACCGTGGAGA AGAAGGAGCC CTGGGCGATA
    AAACGTGCCA TTGAGAAAAC TCTGGAGAGA AATCTTCAAC TTCGACTGTC GAACCAAGAC
    TGGGCAAAGA AACTGGTGGG TTTCGGAAGC GATGGAGCTC CAGGTTCAGA GGGAGAAAAC
    GGCAGCACAG TGGCCCTGCT TTTAAGGGAA ATCCAGCCAG GTGTGCTGCC TGTTTACTGC
    CTTGCCCATC ACCTCGAACT GTCTTACCAG GCGGTGTTCC AGAGCGTTCC CCTCTACAGC
    CGTGTGAAAG ACCTTCTTCA TAGCATGCAC CGCTTCTATC ACGATTCTCC TGCTCACAGG
    AGTGCTCTGG TGACTGCTTT CAAGGGCCTC CACCTTCAAC CTGCGATGCC CTCTCAAGTC
    AGAGGCCGGA GGTGGCTTCA GGGATTGCAG GCTGCCCTCC AGAACTTTCT CAAGGGATAT
    CCAGCAATTG TCCAGCAGCT GCAGTCAACA AAAGAAAGCA GAAGCAACAG CAGTCATCAG
    AAAGCCGAAG AACTCCTGGA GTCTCTCCTC CAGGCAGACA TTGTCAAATT TGTCCACTTC
    TTGGTGGATG TCGTCAATGT CCTTAGCATA CTGTCCCATG TCAATCAGAA CAGAAATTCC
    TCAATTGCTG ATGTATTTGC CACCTTAGAG TCAACACTGG AAATGCTCCA AATTTATCAG
    ACAAGACCAG GGCCGAAAGA ACGTGGGGTA GATTCGGTCA CACACTTTCA TGGTAACTGC
    CTTGGAGGAA AGGGAAACAT TTCTGACGTG AGAAACATTG TTTTAACACA TCTTATCAAG
    AGGCTGCGAG GCTGTTTCAG AGATGCCAAC CAAGATGTGG TGAAGGCAAC CACGATTGGA
    AGCTTTAAAC TGTGGCCGGC AAAAATAAAT CAAGAATTTG GAGAAAAAGA GGTGGCCATC
    CTGATTGCAC ATTATGAACC AGTATTAGAG GCAGCCAAGG TGAAAATTGA CCAAGTAGAC
    ACAGAATGGA GCATGTTGAA ATTGGAGATC TACTCTAGAT TTCAGAACAT TTGCAAACTG
    ACCTGGGATT TTGTGAATTC TGTTTATTTA CACAAGTATC CAAATATCCT GACACTCATT
    GACCTCGTGC TGAGCCTGCC TGCAAGTTCA GCTGAGGCAG GACGTGGCTT TAGTCAGATG
    AAGCGCATCA GGTCCCAAAT GCATGCCAAG GTCAAGGCTG ACAACATGAC GGATCTCCTG
    ATAATTCAGT TGAATTCTCC AGACATTAAC AACTTTGACC CTAGGAAAGC CATTCATTTA
    TGGAATACAA GAACACCATC CTCAACTGAG GACACAAGAC CTAACTCAGA CTGCTCATCA
    AACTCAGAAA GCCTTAGTGA AAGCGATGAG TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OOv13484
    Clone ID Related Accession (Same CDS sequence) XM_015106490.1
    Accession Version XM_015106490.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6336bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-16
    Organism Ovis aries musimon(Ovis orientalis musimon)
    Product sperm flagellar protein 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942355.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGTCGGAAA TCCTGTGCCA GTGGCTCAAC CAGGAATTGA AGGTGTCCCA GACCGTGAGT 
    CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAA TTGGAGAAGT TTTGCACAAG
    TTTGCACTTC AGGATGATTT TGCAGACTTT TCAGAAAGCA GGATTTCCAG TGCCAGACTT
    AACAATTTTT CTCGCTTGGA GCCCACACTT CACCTTTTGG GTGTGGAATT TGATCAGAAT
    GTGGCCCACA ATATCATCAC AGAGAAGCCT GGGGCTGCCA CGAAACTCTT ATATCAGTTG
    TACATTGCTC TCCAGAGGAA AAAGAAAGGT GTTCAGACTA GAGTGGAAAT GCCAACTGTA
    CAGCCCCTGA TGAGCACAAA AGTTCAAAAC ATGAAAAGCG AGGCTTTCCG AGATCAACGC
    TTGAGCAAAA GACGACAAAA TGAAATCATG GCCAAAATCC AAGCAGCTAT CATACAGATT
    CCTAAACCCA CATCAAATCG TACTCTGAAA ACATTTGACG CCCAAAAATT AATGAAAAAG
    AAAAAAGAGG CAGAGGATGT GGCCAATGAA ATTAAGAAGT TTGAAGCATT AATAAAAAAG
    GATCTCCAGG CAAAAGAAAG TGCATCCAAG ACTTCTCTAG ATACAGCAGG CCAGACAACC
    GCTGATTTGT TAAACACTTA TTCAGATGAT GACTACATTA AAAAAATCCA AAAACGCCTT
    GAAGAAGATG CTTTTGCACG AGAGCAAAGG GAGAAAAGAC GGCGGAGACT CTTAATGGAT
    CAGTTAATAG CTCATGAAGC CCAAGAGGAG GCTTATCGAG AGGAGCAGTT GATCAACCGG
    CTGATGCGAC AGTCCCAGCA GGAACGCAGG ATCGCTGTGC AGCTCATGCA CGTTAGGCAC
    GAAAAGGAAG TTTTATGGCA AAACAGAATT TTCAGAGAAA AACAATATGA GGAAAGACGG
    CTTAAAGACT TCCAGGATGC TCTTGATCGA GAAGCGGCTT TAGCCAGACA AGCCAAGATT
    GATTTTGAAG AACAAGCCCT CAGAGATAAG GAAATTCATG AGCAGATCGC CATAGAAAGA
    GCTCAAGCTC GTTACAAAAA GCATTATTCA ATATGTGCAG AAATTTTGGA TCAGATACTT
    GATCTGTCCA CTAAGGTGGC AGACTATCGA TTGTTGACAA ATAACCTGAT CCCATATAAG
    TTGATGCATG ATTGGAAGGA ACTGTTTTTC CATGGAAAAC CCATATATGA GCAAGCCTCT
    CTTAGACATT TACCAGCTAA GCTCTCTGCA GAACACCTTG TAGAAATGGA GAAAAGGAAC
    TTGCTAGATA ACAATGATTA TGAAGAATAT AAGAACATGG TTGGAGAATG GGCCTTACCA
    GAAGAAATGG TTGAAAACTT ACCACCCTCC AACAATTGCA TATTGGGCCA TGTGATTCAC
    AGACTAATTG AAAGTTCTCT TCCTCCTCAA GTTGAATCTA CAGTACCTGA GTTACCTTCA
    TTTGCTATAA AAGGATGCTT GTTGGGGAAA ACCTTTAGTG GAAAAACTAC TGCTCTAAAG
    GCTCTAGAGA AAAGCTTTCC TATTCAGGTA CTTTCTATTG ACACTCTTGT TCAAGAAGCT
    ATCCAAGCAT TTCATGACAA TGAAAGTATC AGCGGGGCCC ATGGACTTCA GAAAGCACCT
    GAAGAAAAGG TTTTACCAGT TAGGCGAAAG AGTGGTAAAG AAAACCAGGA CGTGCAAGAT
    GAAGCACTAC CAGAAAGAGA AGATAAAACC AGACTTGATA CCGATGCTAG AAAAGCAGCA
    AAAGCTGAAG AAGTCGTAGC GAGTGATAGT TTCTCTGAAC TCACTGTGCG TGCTCAGCTT
    GGTGCAAAAT CAGAAATGCT GCTGAAGAAA GGAAAGAGCA TATCTGATAT ATTGCTAGTC
    AGCATCATGG TAAATGCCAT TAATCAGATA CCTATGTATA AAGGCTGGAT CCTGGATGGC
    TTTCCAATGA CATTAAACCA AGCAAAACTT CTGGAGGAAG CACTCACAGG CTACAACAGA
    AACCTCATAG AACTGGAGGG AAAGAAATCA CAAACATCCA CATTAGCTGT TGACCCTACA
    GCTTCCAAAG AAGTGCCTCT TCCCCCTTCT GCATTTGATT TTGTCATGTT ATTAGACATT
    TCAGATAATT CCTCACTGAA TCGCATGAAT GACATCATGG CTAAAGCATT TTCAAGTGAA
    GCTCCTCGTG AAGATATCAA TCAGCGTGTA GCTGCTAAAA ACAAAGATAC AGATGAGGAC
    CAGAATTTAA GAGATCAAAT ACAGCACAGA ATTGTAGGCT TCCTGGACAA CTGGCCTTCG
    TTGGAGCAGT GGTTTACAGA GCCAGAAAAC ATTTTGATAA AAATCAATGC TGAAGTAGAT
    AAACAGTCAT TGTTCCAAAA AGTAAAGGAA ATGATTATGA CTGAAATAAT TAAAAAAGAA
    AATAAAGTGA AAAAGAAATT AGAAGAAAAG GAAGCTGAGA GAAAAGAAGA AGTTTCCCTG
    AGTGAGAAGA CATCCCCTCC TCCTCCTCCC CCTCCGGAAC CAGAGAAAGA GAAGGAAGTC
    CACCTTCCAC AGGAGCGGTC CAAAACTCCT GCTGCAAAAG GAAAGCAAGC ATCAGAACCC
    CTGCATGGTA ATCGAGAACC TCTTCAGGAA GGAAAAGGGA AGAAAAGTGA AACTTCACTC
    AAAAGAAAAG GTTCTCCTAG AGGAAAATCG CTAGGAGGGA AGATTCCACT CAAGAAATCA
    CCTGCCGAGT CTACAGATAT TTCACCGGTC CCGGTAGTGC CAGCCCCATC GAAGCCCGGA
    TCAGAAGAGT GGGTGTATGT AAATGAACCA ATTCCTGAGG AAATACCATC ATTTTTAGTA
    CCATACTGGA AATTGATAGA AAATTCCTAT ATAAATTCCA TCAAAACAGT ACTCAGGGAG
    TTGAGAGAAG GCCAGCATAG TGTACTTGCT TACTTATATG ATATTAGAAC AGGCTTCCAG
    CAGTTTCTGA AGCGTCCAGA CCACAAGCAA GACTTCGTGT CTCAGTGGCA GGCGGATTTC
    AACTCCCTTC CTGATGACCT GTGGGATGAT GAGGAAACAA AGGCTGAGCT GCACCAAAGA
    GTGAATGATT TACGGGACCG CCTGTGGGAC ATTTGCGATG CCCGTAAGGA AGAAGCAGAG
    CAGGAGCGTC TGGACATCAT TAACGAGAGC TGGTTGCAGG ACTCAATGGG AATTGCCATG
    AACCATTTCT TCTCACTGAT GCAGGCAGAA CTGAACCGTT TCCAAGATAC GAAGAGATTC
    CTGCAAGATT ATTACAGGGC CATGGAATGT AAAATTCCGA TGGATGACAC TAAGAAATTC
    ACTCGAGTCC CACTGGTCCG ACTGGATAGT AGAGATATTT TGGAAAGCCA GCTTAGAATT
    CCCCTGGTTC CTCGAAGATC CATTTCTCCA GAGTCAGCTT TATCCAAACC AAAAACAAAA
    AGCATCCTGA AGGGCAAGAT CGATTACACG CTGGAAAGTG TAGAGTTAAA CTTTGAGACT
    GATGAGAAGA TGGTGATGGA TACGTGGCAG CAGTCTTCTT TGGCAGTATC TCACATGGTG
    GCTGCTGAAA TTCATCAGAG GCTCATGGAA GAAGAAAAAG AAAACCAGCC AGCAGAAACA
    AAAGAAAAAC TTCCTCAGGC AGCTGCAAAT AAAAAAGTGA AAAAGGAGCC TCCGCCACCG
    CCACCACCAC CCAAGAAAAA GAAAGAGGAC AAAAAAGGAA AAGGTAAATC CCCACCTATG
    GCAGAAGCTA CTCCGGTAGT AGTGCCAGCA GAAGAGACTG AGGAAGTGGA ATTAAAAAAT
    GAACTGAAGC TCAGAATAAA GGAAGAGCAT CTTGCTGCCC TGCAATTTGA AGAGATAGCT
    ACACAGTTCC GACTTGAACT GATAAAGGTA AAAGCATTGG CTTTCCTGGA AGAGTTAGTA
    ATAAAGGCGG TTGATGTATA TAGACTCATG GACAAGTGGC TCGGTGAGAG GTACTTGAAT
    GAAATGGCCA GTGTGGAAAA ACTAACTGGG GTAGCTCGCT ATCACATTGA AACATCAACC
    AAAATTCAGA ATGAACTTTA TTTAGACCAA GAAGACTTCT TCATTAATGG CGATATCAAA
    GTCTTTCCAG ATCCACCTCC TCCGGTACGC CCTCCGCCTG TGGAAAAGGA GGAAAATGGC
    ACCCTGACCA TGGAGCAGCT TGACAACCTC CGGGATCAGT TCTTAGATAT AGCGCCCAAA
    GGCAGGGATC CTCCAGACAA TGTGAGTGGA TTCACCAAGA AGCAGAAAAG GCCTCGGAAG
    CTTGACCATT ACCTCCCAAA GAGGAACAAT CAATTTTTGA AAATTTGGCT TAAAAAATAC
    CCTTGGCTTG TATACGATGA GATACTAAAT CTTATGTTCT GCGGCCTGTG TCGTAAACAT
    GGAGTGACAT TAAAGGGAAA TCAGGTCAGT TTTTTTTATG GCACGGATAA CTTCAGGACT
    GAATTTCTCA ACGCGCATCA TCTGAGTGAG GCTCACGCCA AGGCGTCCCT CATGGAAGCC
    ACGAGTGGTT CTCCAGGGAG CAGAGCTACC ACGGAGCTGA TGGTGAGGAC CATGAGCAAG
    GTGACCCTTG GCAGGGTGGA GAACCTGTTC CGGTCCTGCC ACGCCATCGC CAAGACCGGA
    CGTCCCCTCA AAGACTTCCT CTGGATGTGT TCGCTGGACG ATATGAAGGG AGTTGATATC
    GGCCCGGTGT TCAGAACCAG TAAATCGGCC AGAACTTTTA CCTACTTTAT TGCTGAAGTG
    GAACGGAGAA CTCTAAGAGA GAAGTTAGAA AAAAGTCAGT TCTTCTCCGT CCTCAGTGAT
    GGGATTGCAG ACAGTTCAGC TGAGGAAGCT GAACTCGTAT ATGTGCAGTT TGCCCGCGCA
    GGGAGAGTGC ACTGCCAAAT CGTCGGGGTA CAGACCGTGG AGAAGAAGGA GCCCTGGGCA
    ATAAAACGTG CCATCGAGAA AACTCTGGAG AGAAATCTTC AACTTCGACT GTCGAACCAA
    GACTGGGCAA AGAAACTGGT GGGTTTCGGA AGCGATGGAG CTCCAGGTTC AGAGGGAGAA
    AACGGCAGCA CAGTGGCCCT GCTTTTAAGG GAAATCCAGC CAGGTGTGCT GCCTGTTTAC
    TGCCTTGCCC ATCACCTCGA ACTGTCTTAC CAGGCGGTGT TCCAGAGCGT TCCCCTCTAC
    AGCCGTGTGA AAGACCTTCT TCATAGCATG CACCGCTTCT ATCACGATTC TCCTGCTCAC
    AGGAGTGCTC TGGTGACTGC TTTCAAGGGC CTCCACCTTC AACCTGCGAT GCCCTCTCAA
    GTCAGAGGCC GGAGGTGGCT TCAGGGATTG CAGGCTGCCC TCCAGAACTT TCTCAAGGGA
    TATCCAGCAA TTGTCCAGCA GCTGCAGTCA ACAGAAGAAA GCAGAAGCAA CAGCAGTCAT
    CAGAAAGCCG AAGAACTCCT GGAGTCTCTC CTCCAGGCAG ACATTGTCAA ATTTGTCCAC
    TTCTTGGTGG ATGTCGTCAA TGTCCTTAGC ATACTGTCCC ATGTCAATCA GAACAGAAAT
    TCCTCAATTG CTGATGTATT TGCCACCTTA GAGTCAACAC TGGAAATGCT CCAAATTTAT
    CAGACAAGAC CAGGGCCGAA AGAACGTGGG GTAGATTCGG TCACACACTT TCATGGTAAC
    TGCCTTGGAG GAAAGGGAAA CATTTCTGAC GTGAGAAACA TTGTTTTAAC ACATCTTATC
    AAGAGGCTGC GAGGCTGTTT CAGAGATGCC AACCAAGATG TGGTGAAGGC AACCACGGTT
    GGAAGCTTTA AACTGTGGCC GGCAAAAATA AATCAAGAAT TTGGAGAAAA AGAGGTGGCC
    ATCCTGATTG CACATTATGA ACCAGTATTA GAGGCAGCCA AGGTGAAAAT TGACCAAGTA
    GACACAGAAT GGAGCATGTT GAAATTGGAG ATCTACTCTA GATTTCAGAA CATTTGCAAA
    CTGACCTGGG ATTTTGTGAA TTCTGTTTAT TTACACAAGT ATCCAAACAT CCTGACACTC
    ATTGACCTTG TGCTGAGCCT GCCTGCAAGT TCAGCTGAGG CAGGACGTGG CTTTAGTCAG
    ATGAAGCGCA TCAGGTCCCA AATGCATGCC AAGGTCAAGG CTGACAACAT GACGGATCTC
    CTGATAATTC AGTTGAATTC TCCAGACATT AACAACTTTG ACCCTAGGAA AGCCATTCAT
    TTATGGAATA CAAGAACACC ATCCTCAACT GAGGACACAA GACCTAACTC AGACTGCTCA
    TCAAACTCAG AAAGCCTTAG TGAAAGCGAT AAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_014961976.1
    CDS497..6832
    Translation

    Target ORF information:

    RefSeq Version XM_015106490.1
    Organism Ovis aries musimon(Ovis orientalis musimon)
    Definition Ovis aries musimon sperm flagellar 2 (SPEF2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015106490.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGTCGGAAA TCCTGTGCCA GTGGCTCAAC CAGGAATTGA AGGTGTCCCA GACCGTGAGT 
    CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAA TTGGAGAAGT TTTGCACAAG
    TTTGCACTTC AGGATGATTT TGCAGACTTT TCAGAAAGCA GGATTTCCAG TGCCAGACTT
    AACAATTTTT CTCGCTTGGA GCCCACACTT CACCTTTTGG GTGTGGAATT TGATCAGAAT
    GTGGCCCACA ATATCATCAC AGAGAAGCCT GGGGCTGCCA CGAAACTCTT ATATCAGTTG
    TACATTGCTC TCCAGAGGAA AAAGAAAGGT GTTCAGACTA GAGTGGAAAT GCCAACTGTA
    CAGCCCCTGA TGAGCACAAA AGTTCAAAAC ATGAAAAGCG AGGCTTTCCG AGATCAACGC
    TTGAGCAAAA GACGACAAAA TGAAATCATG GCCAAAATCC AAGCAGCTAT CATACAGATT
    CCTAAACCCA CATCAAATCG TACTCTGAAA ACATTTGACG CCCAAAAATT AATGAAAAAG
    AAAAAAGAGG CAGAGGATGT GGCCAATGAA ATTAAGAAGT TTGAAGCATT AATAAAAAAG
    GATCTCCAGG CAAAAGAAAG TGCATCCAAG ACTTCTCTAG ATACAGCAGG CCAGACAACC
    GCTGATTTGT TAAACACTTA TTCAGATGAT GACTACATTA AAAAAATCCA AAAACGCCTT
    GAAGAAGATG CTTTTGCACG AGAGCAAAGG GAGAAAAGAC GGCGGAGACT CTTAATGGAT
    CAGTTAATAG CTCATGAAGC CCAAGAGGAG GCTTATCGAG AGGAGCAGTT GATCAACCGG
    CTGATGCGAC AGTCCCAGCA GGAACGCAGG ATCGCTGTGC AGCTCATGCA CGTTAGGCAC
    GAAAAGGAAG TTTTATGGCA AAACAGAATT TTCAGAGAAA AACAATATGA GGAAAGACGG
    CTTAAAGACT TCCAGGATGC TCTTGATCGA GAAGCGGCTT TAGCCAGACA AGCCAAGATT
    GATTTTGAAG AACAAGCCCT CAGAGATAAG GAAATTCATG AGCAGATCGC CATAGAAAGA
    GCTCAAGCTC GTTACAAAAA GCATTATTCA ATATGTGCAG AAATTTTGGA TCAGATACTT
    GATCTGTCCA CTAAGGTGGC AGACTATCGA TTGTTGACAA ATAACCTGAT CCCATATAAG
    TTGATGCATG ATTGGAAGGA ACTGTTTTTC CATGGAAAAC CCATATATGA GCAAGCCTCT
    CTTAGACATT TACCAGCTAA GCTCTCTGCA GAACACCTTG TAGAAATGGA GAAAAGGAAC
    TTGCTAGATA ACAATGATTA TGAAGAATAT AAGAACATGG TTGGAGAATG GGCCTTACCA
    GAAGAAATGG TTGAAAACTT ACCACCCTCC AACAATTGCA TATTGGGCCA TGTGATTCAC
    AGACTAATTG AAAGTTCTCT TCCTCCTCAA GTTGAATCTA CAGTACCTGA GTTACCTTCA
    TTTGCTATAA AAGGATGCTT GTTGGGGAAA ACCTTTAGTG GAAAAACTAC TGCTCTAAAG
    GCTCTAGAGA AAAGCTTTCC TATTCAGGTA CTTTCTATTG ACACTCTTGT TCAAGAAGCT
    ATCCAAGCAT TTCATGACAA TGAAAGTATC AGCGGGGCCC ATGGACTTCA GAAAGCACCT
    GAAGAAAAGG TTTTACCAGT TAGGCGAAAG AGTGGTAAAG AAAACCAGGA CGTGCAAGAT
    GAAGCACTAC CAGAAAGAGA AGATAAAACC AGACTTGATA CCGATGCTAG AAAAGCAGCA
    AAAGCTGAAG AAGTCGTAGC GAGTGATAGT TTCTCTGAAC TCACTGTGCG TGCTCAGCTT
    GGTGCAAAAT CAGAAATGCT GCTGAAGAAA GGAAAGAGCA TATCTGATAT ATTGCTAGTC
    AGCATCATGG TAAATGCCAT TAATCAGATA CCTATGTATA AAGGCTGGAT CCTGGATGGC
    TTTCCAATGA CATTAAACCA AGCAAAACTT CTGGAGGAAG CACTCACAGG CTACAACAGA
    AACCTCATAG AACTGGAGGG AAAGAAATCA CAAACATCCA CATTAGCTGT TGACCCTACA
    GCTTCCAAAG AAGTGCCTCT TCCCCCTTCT GCATTTGATT TTGTCATGTT ATTAGACATT
    TCAGATAATT CCTCACTGAA TCGCATGAAT GACATCATGG CTAAAGCATT TTCAAGTGAA
    GCTCCTCGTG AAGATATCAA TCAGCGTGTA GCTGCTAAAA ACAAAGATAC AGATGAGGAC
    CAGAATTTAA GAGATCAAAT ACAGCACAGA ATTGTAGGCT TCCTGGACAA CTGGCCTTCG
    TTGGAGCAGT GGTTTACAGA GCCAGAAAAC ATTTTGATAA AAATCAATGC TGAAGTAGAT
    AAACAGTCAT TGTTCCAAAA AGTAAAGGAA ATGATTATGA CTGAAATAAT TAAAAAAGAA
    AATAAAGTGA AAAAGAAATT AGAAGAAAAG GAAGCTGAGA GAAAAGAAGA AGTTTCCCTG
    AGTGAGAAGA CATCCCCTCC TCCTCCTCCC CCTCCGGAAC CAGAGAAAGA GAAGGAAGTC
    CACCTTCCAC AGGAGCGGTC CAAAACTCCT GCTGCAAAAG GAAAGCAAGC ATCAGAACCC
    CTGCATGGTA ATCGAGAACC TCTTCAGGAA GGAAAAGGGA AGAAAAGTGA AACTTCACTC
    AAAAGAAAAG GTTCTCCTAG AGGAAAATCG CTAGGAGGGA AGATTCCACT CAAGAAATCA
    CCTGCCGAGT CTACAGATAT TTCACCGGTC CCGGTAGTGC CAGCCCCATC GAAGCCCGGA
    TCAGAAGAGT GGGTGTATGT AAATGAACCA ATTCCTGAGG AAATACCATC ATTTTTAGTA
    CCATACTGGA AATTGATAGA AAATTCCTAT ATAAATTCCA TCAAAACAGT ACTCAGGGAG
    TTGAGAGAAG GCCAGCATAG TGTACTTGCT TACTTATATG ATATTAGAAC AGGCTTCCAG
    CAGTTTCTGA AGCGTCCAGA CCACAAGCAA GACTTCGTGT CTCAGTGGCA GGCGGATTTC
    AACTCCCTTC CTGATGACCT GTGGGATGAT GAGGAAACAA AGGCTGAGCT GCACCAAAGA
    GTGAATGATT TACGGGACCG CCTGTGGGAC ATTTGCGATG CCCGTAAGGA AGAAGCAGAG
    CAGGAGCGTC TGGACATCAT TAACGAGAGC TGGTTGCAGG ACTCAATGGG AATTGCCATG
    AACCATTTCT TCTCACTGAT GCAGGCAGAA CTGAACCGTT TCCAAGATAC GAAGAGATTC
    CTGCAAGATT ATTACAGGGC CATGGAATGT AAAATTCCGA TGGATGACAC TAAGAAATTC
    ACTCGAGTCC CACTGGTCCG ACTGGATAGT AGAGATATTT TGGAAAGCCA GCTTAGAATT
    CCCCTGGTTC CTCGAAGATC CATTTCTCCA GAGTCAGCTT TATCCAAACC AAAAACAAAA
    AGCATCCTGA AGGGCAAGAT CGATTACACG CTGGAAAGTG TAGAGTTAAA CTTTGAGACT
    GATGAGAAGA TGGTGATGGA TACGTGGCAG CAGTCTTCTT TGGCAGTATC TCACATGGTG
    GCTGCTGAAA TTCATCAGAG GCTCATGGAA GAAGAAAAAG AAAACCAGCC AGCAGAAACA
    AAAGAAAAAC TTCCTCAGGC AGCTGCAAAT AAAAAAGTGA AAAAGGAGCC TCCGCCACCG
    CCACCACCAC CCAAGAAAAA GAAAGAGGAC AAAAAAGGAA AAGGTAAATC CCCACCTATG
    GCAGAAGCTA CTCCGGTAGT AGTGCCAGCA GAAGAGACTG AGGAAGTGGA ATTAAAAAAT
    GAACTGAAGC TCAGAATAAA GGAAGAGCAT CTTGCTGCCC TGCAATTTGA AGAGATAGCT
    ACACAGTTCC GACTTGAACT GATAAAGGTA AAAGCATTGG CTTTCCTGGA AGAGTTAGTA
    ATAAAGGCGG TTGATGTATA TAGACTCATG GACAAGTGGC TCGGTGAGAG GTACTTGAAT
    GAAATGGCCA GTGTGGAAAA ACTAACTGGG GTAGCTCGCT ATCACATTGA AACATCAACC
    AAAATTCAGA ATGAACTTTA TTTAGACCAA GAAGACTTCT TCATTAATGG CGATATCAAA
    GTCTTTCCAG ATCCACCTCC TCCGGTACGC CCTCCGCCTG TGGAAAAGGA GGAAAATGGC
    ACCCTGACCA TGGAGCAGCT TGACAACCTC CGGGATCAGT TCTTAGATAT AGCGCCCAAA
    GGCAGGGATC CTCCAGACAA TGTGAGTGGA TTCACCAAGA AGCAGAAAAG GCCTCGGAAG
    CTTGACCATT ACCTCCCAAA GAGGAACAAT CAATTTTTGA AAATTTGGCT TAAAAAATAC
    CCTTGGCTTG TATACGATGA GATACTAAAT CTTATGTTCT GCGGCCTGTG TCGTAAACAT
    GGAGTGACAT TAAAGGGAAA TCAGGTCAGT TTTTTTTATG GCACGGATAA CTTCAGGACT
    GAATTTCTCA ACGCGCATCA TCTGAGTGAG GCTCACGCCA AGGCGTCCCT CATGGAAGCC
    ACGAGTGGTT CTCCAGGGAG CAGAGCTACC ACGGAGCTGA TGGTGAGGAC CATGAGCAAG
    GTGACCCTTG GCAGGGTGGA GAACCTGTTC CGGTCCTGCC ACGCCATCGC CAAGACCGGA
    CGTCCCCTCA AAGACTTCCT CTGGATGTGT TCGCTGGACG ATATGAAGGG AGTTGATATC
    GGCCCGGTGT TCAGAACCAG TAAATCGGCC AGAACTTTTA CCTACTTTAT TGCTGAAGTG
    GAACGGAGAA CTCTAAGAGA GAAGTTAGAA AAAAGTCAGT TCTTCTCCGT CCTCAGTGAT
    GGGATTGCAG ACAGTTCAGC TGAGGAAGCT GAACTCGTAT ATGTGCAGTT TGCCCGCGCA
    GGGAGAGTGC ACTGCCAAAT CGTCGGGGTA CAGACCGTGG AGAAGAAGGA GCCCTGGGCA
    ATAAAACGTG CCATCGAGAA AACTCTGGAG AGAAATCTTC AACTTCGACT GTCGAACCAA
    GACTGGGCAA AGAAACTGGT GGGTTTCGGA AGCGATGGAG CTCCAGGTTC AGAGGGAGAA
    AACGGCAGCA CAGTGGCCCT GCTTTTAAGG GAAATCCAGC CAGGTGTGCT GCCTGTTTAC
    TGCCTTGCCC ATCACCTCGA ACTGTCTTAC CAGGCGGTGT TCCAGAGCGT TCCCCTCTAC
    AGCCGTGTGA AAGACCTTCT TCATAGCATG CACCGCTTCT ATCACGATTC TCCTGCTCAC
    AGGAGTGCTC TGGTGACTGC TTTCAAGGGC CTCCACCTTC AACCTGCGAT GCCCTCTCAA
    GTCAGAGGCC GGAGGTGGCT TCAGGGATTG CAGGCTGCCC TCCAGAACTT TCTCAAGGGA
    TATCCAGCAA TTGTCCAGCA GCTGCAGTCA ACAGAAGAAA GCAGAAGCAA CAGCAGTCAT
    CAGAAAGCCG AAGAACTCCT GGAGTCTCTC CTCCAGGCAG ACATTGTCAA ATTTGTCCAC
    TTCTTGGTGG ATGTCGTCAA TGTCCTTAGC ATACTGTCCC ATGTCAATCA GAACAGAAAT
    TCCTCAATTG CTGATGTATT TGCCACCTTA GAGTCAACAC TGGAAATGCT CCAAATTTAT
    CAGACAAGAC CAGGGCCGAA AGAACGTGGG GTAGATTCGG TCACACACTT TCATGGTAAC
    TGCCTTGGAG GAAAGGGAAA CATTTCTGAC GTGAGAAACA TTGTTTTAAC ACATCTTATC
    AAGAGGCTGC GAGGCTGTTT CAGAGATGCC AACCAAGATG TGGTGAAGGC AACCACGGTT
    GGAAGCTTTA AACTGTGGCC GGCAAAAATA AATCAAGAAT TTGGAGAAAA AGAGGTGGCC
    ATCCTGATTG CACATTATGA ACCAGTATTA GAGGCAGCCA AGGTGAAAAT TGACCAAGTA
    GACACAGAAT GGAGCATGTT GAAATTGGAG ATCTACTCTA GATTTCAGAA CATTTGCAAA
    CTGACCTGGG ATTTTGTGAA TTCTGTTTAT TTACACAAGT ATCCAAACAT CCTGACACTC
    ATTGACCTTG TGCTGAGCCT GCCTGCAAGT TCAGCTGAGG CAGGACGTGG CTTTAGTCAG
    ATGAAGCGCA TCAGGTCCCA AATGCATGCC AAGGTCAAGG CTGACAACAT GACGGATCTC
    CTGATAATTC AGTTGAATTC TCCAGACATT AACAACTTTG ACCCTAGGAA AGCCATTCAT
    TTATGGAATA CAAGAACACC ATCCTCAACT GAGGACACAA GACCTAACTC AGACTGCTCA
    TCAAACTCAG AAAGCCTTAG TGAAAGCGAT AAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OOv13485
    Clone ID Related Accession (Same CDS sequence) XM_015106491.1
    Accession Version XM_015106491.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6285bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-16
    Organism Ovis aries musimon(Ovis orientalis musimon)
    Product sperm flagellar protein 2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942355.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGCAAGGTC CCAAGTCATT TGCAAAGGCA TTTTCCAGTG GCTATCTAAT TGGAGAAGTT 
    TTGCACAAGT TTGCACTTCA GGATGATTTT GCAGACTTTT CAGAAAGCAG GATTTCCAGT
    GCCAGACTTA ACAATTTTTC TCGCTTGGAG CCCACACTTC ACCTTTTGGG TGTGGAATTT
    GATCAGAATG TGGCCCACAA TATCATCACA GAGAAGCCTG GGGCTGCCAC GAAACTCTTA
    TATCAGTTGT ACATTGCTCT CCAGAGGAAA AAGAAAGGTG TTCAGACTAG AGTGGAAATG
    CCAACTGTAC AGCCCCTGAT GAGCACAAAA GTTCAAAACA TGAAAAGCGA GGCTTTCCGA
    GATCAACGCT TGAGCAAAAG ACGACAAAAT GAAATCATGG CCAAAATCCA AGCAGCTATC
    ATACAGATTC CTAAACCCAC ATCAAATCGT ACTCTGAAAA CATTTGACGC CCAAAAATTA
    ATGAAAAAGA AAAAAGAGGC AGAGGATGTG GCCAATGAAA TTAAGAAGTT TGAAGCATTA
    ATAAAAAAGG ATCTCCAGGC AAAAGAAAGT GCATCCAAGA CTTCTCTAGA TACAGCAGGC
    CAGACAACCG CTGATTTGTT AAACACTTAT TCAGATGATG ACTACATTAA AAAAATCCAA
    AAACGCCTTG AAGAAGATGC TTTTGCACGA GAGCAAAGGG AGAAAAGACG GCGGAGACTC
    TTAATGGATC AGTTAATAGC TCATGAAGCC CAAGAGGAGG CTTATCGAGA GGAGCAGTTG
    ATCAACCGGC TGATGCGACA GTCCCAGCAG GAACGCAGGA TCGCTGTGCA GCTCATGCAC
    GTTAGGCACG AAAAGGAAGT TTTATGGCAA AACAGAATTT TCAGAGAAAA ACAATATGAG
    GAAAGACGGC TTAAAGACTT CCAGGATGCT CTTGATCGAG AAGCGGCTTT AGCCAGACAA
    GCCAAGATTG ATTTTGAAGA ACAAGCCCTC AGAGATAAGG AAATTCATGA GCAGATCGCC
    ATAGAAAGAG CTCAAGCTCG TTACAAAAAG CATTATTCAA TATGTGCAGA AATTTTGGAT
    CAGATACTTG ATCTGTCCAC TAAGGTGGCA GACTATCGAT TGTTGACAAA TAACCTGATC
    CCATATAAGT TGATGCATGA TTGGAAGGAA CTGTTTTTCC ATGGAAAACC CATATATGAG
    CAAGCCTCTC TTAGACATTT ACCAGCTAAG CTCTCTGCAG AACACCTTGT AGAAATGGAG
    AAAAGGAACT TGCTAGATAA CAATGATTAT GAAGAATATA AGAACATGGT TGGAGAATGG
    GCCTTACCAG AAGAAATGGT TGAAAACTTA CCACCCTCCA ACAATTGCAT ATTGGGCCAT
    GTGATTCACA GACTAATTGA AAGTTCTCTT CCTCCTCAAG TTGAATCTAC AGTACCTGAG
    TTACCTTCAT TTGCTATAAA AGGATGCTTG TTGGGGAAAA CCTTTAGTGG AAAAACTACT
    GCTCTAAAGG CTCTAGAGAA AAGCTTTCCT ATTCAGGTAC TTTCTATTGA CACTCTTGTT
    CAAGAAGCTA TCCAAGCATT TCATGACAAT GAAAGTATCA GCGGGGCCCA TGGACTTCAG
    AAAGCACCTG AAGAAAAGGT TTTACCAGTT AGGCGAAAGA GTGGTAAAGA AAACCAGGAC
    GTGCAAGATG AAGCACTACC AGAAAGAGAA GATAAAACCA GACTTGATAC CGATGCTAGA
    AAAGCAGCAA AAGCTGAAGA AGTCGTAGCG AGTGATAGTT TCTCTGAACT CACTGTGCGT
    GCTCAGCTTG GTGCAAAATC AGAAATGCTG CTGAAGAAAG GAAAGAGCAT ATCTGATATA
    TTGCTAGTCA GCATCATGGT AAATGCCATT AATCAGATAC CTATGTATAA AGGCTGGATC
    CTGGATGGCT TTCCAATGAC ATTAAACCAA GCAAAACTTC TGGAGGAAGC ACTCACAGGC
    TACAACAGAA ACCTCATAGA ACTGGAGGGA AAGAAATCAC AAACATCCAC ATTAGCTGTT
    GACCCTACAG CTTCCAAAGA AGTGCCTCTT CCCCCTTCTG CATTTGATTT TGTCATGTTA
    TTAGACATTT CAGATAATTC CTCACTGAAT CGCATGAATG ACATCATGGC TAAAGCATTT
    TCAAGTGAAG CTCCTCGTGA AGATATCAAT CAGCGTGTAG CTGCTAAAAA CAAAGATACA
    GATGAGGACC AGAATTTAAG AGATCAAATA CAGCACAGAA TTGTAGGCTT CCTGGACAAC
    TGGCCTTCGT TGGAGCAGTG GTTTACAGAG CCAGAAAACA TTTTGATAAA AATCAATGCT
    GAAGTAGATA AACAGTCATT GTTCCAAAAA GTAAAGGAAA TGATTATGAC TGAAATAATT
    AAAAAAGAAA ATAAAGTGAA AAAGAAATTA GAAGAAAAGG AAGCTGAGAG AAAAGAAGAA
    GTTTCCCTGA GTGAGAAGAC ATCCCCTCCT CCTCCTCCCC CTCCGGAACC AGAGAAAGAG
    AAGGAAGTCC ACCTTCCACA GGAGCGGTCC AAAACTCCTG CTGCAAAAGG AAAGCAAGCA
    TCAGAACCCC TGCATGGTAA TCGAGAACCT CTTCAGGAAG GAAAAGGGAA GAAAAGTGAA
    ACTTCACTCA AAAGAAAAGG TTCTCCTAGA GGAAAATCGC TAGGAGGGAA GATTCCACTC
    AAGAAATCAC CTGCCGAGTC TACAGATATT TCACCGGTCC CGGTAGTGCC AGCCCCATCG
    AAGCCCGGAT CAGAAGAGTG GGTGTATGTA AATGAACCAA TTCCTGAGGA AATACCATCA
    TTTTTAGTAC CATACTGGAA ATTGATAGAA AATTCCTATA TAAATTCCAT CAAAACAGTA
    CTCAGGGAGT TGAGAGAAGG CCAGCATAGT GTACTTGCTT ACTTATATGA TATTAGAACA
    GGCTTCCAGC AGTTTCTGAA GCGTCCAGAC CACAAGCAAG ACTTCGTGTC TCAGTGGCAG
    GCGGATTTCA ACTCCCTTCC TGATGACCTG TGGGATGATG AGGAAACAAA GGCTGAGCTG
    CACCAAAGAG TGAATGATTT ACGGGACCGC CTGTGGGACA TTTGCGATGC CCGTAAGGAA
    GAAGCAGAGC AGGAGCGTCT GGACATCATT AACGAGAGCT GGTTGCAGGA CTCAATGGGA
    ATTGCCATGA ACCATTTCTT CTCACTGATG CAGGCAGAAC TGAACCGTTT CCAAGATACG
    AAGAGATTCC TGCAAGATTA TTACAGGGCC ATGGAATGTA AAATTCCGAT GGATGACACT
    AAGAAATTCA CTCGAGTCCC ACTGGTCCGA CTGGATAGTA GAGATATTTT GGAAAGCCAG
    CTTAGAATTC CCCTGGTTCC TCGAAGATCC ATTTCTCCAG AGTCAGCTTT ATCCAAACCA
    AAAACAAAAA GCATCCTGAA GGGCAAGATC GATTACACGC TGGAAAGTGT AGAGTTAAAC
    TTTGAGACTG ATGAGAAGAT GGTGATGGAT ACGTGGCAGC AGTCTTCTTT GGCAGTATCT
    CACATGGTGG CTGCTGAAAT TCATCAGAGG CTCATGGAAG AAGAAAAAGA AAACCAGCCA
    GCAGAAACAA AAGAAAAACT TCCTCAGGCA GCTGCAAATA AAAAAGTGAA AAAGGAGCCT
    CCGCCACCGC CACCACCACC CAAGAAAAAG AAAGAGGACA AAAAAGGAAA AGGTAAATCC
    CCACCTATGG CAGAAGCTAC TCCGGTAGTA GTGCCAGCAG AAGAGACTGA GGAAGTGGAA
    TTAAAAAATG AACTGAAGCT CAGAATAAAG GAAGAGCATC TTGCTGCCCT GCAATTTGAA
    GAGATAGCTA CACAGTTCCG ACTTGAACTG ATAAAGGTAA AAGCATTGGC TTTCCTGGAA
    GAGTTAGTAA TAAAGGCGGT TGATGTATAT AGACTCATGG ACAAGTGGCT CGGTGAGAGG
    TACTTGAATG AAATGGCCAG TGTGGAAAAA CTAACTGGGG TAGCTCGCTA TCACATTGAA
    ACATCAACCA AAATTCAGAA TGAACTTTAT TTAGACCAAG AAGACTTCTT CATTAATGGC
    GATATCAAAG TCTTTCCAGA TCCACCTCCT CCGGTACGCC CTCCGCCTGT GGAAAAGGAG
    GAAAATGGCA CCCTGACCAT GGAGCAGCTT GACAACCTCC GGGATCAGTT CTTAGATATA
    GCGCCCAAAG GCAGGGATCC TCCAGACAAT GTGAGTGGAT TCACCAAGAA GCAGAAAAGG
    CCTCGGAAGC TTGACCATTA CCTCCCAAAG AGGAACAATC AATTTTTGAA AATTTGGCTT
    AAAAAATACC CTTGGCTTGT ATACGATGAG ATACTAAATC TTATGTTCTG CGGCCTGTGT
    CGTAAACATG GAGTGACATT AAAGGGAAAT CAGGTCAGTT TTTTTTATGG CACGGATAAC
    TTCAGGACTG AATTTCTCAA CGCGCATCAT CTGAGTGAGG CTCACGCCAA GGCGTCCCTC
    ATGGAAGCCA CGAGTGGTTC TCCAGGGAGC AGAGCTACCA CGGAGCTGAT GGTGAGGACC
    ATGAGCAAGG TGACCCTTGG CAGGGTGGAG AACCTGTTCC GGTCCTGCCA CGCCATCGCC
    AAGACCGGAC GTCCCCTCAA AGACTTCCTC TGGATGTGTT CGCTGGACGA TATGAAGGGA
    GTTGATATCG GCCCGGTGTT CAGAACCAGT AAATCGGCCA GAACTTTTAC CTACTTTATT
    GCTGAAGTGG AACGGAGAAC TCTAAGAGAG AAGTTAGAAA AAAGTCAGTT CTTCTCCGTC
    CTCAGTGATG GGATTGCAGA CAGTTCAGCT GAGGAAGCTG AACTCGTATA TGTGCAGTTT
    GCCCGCGCAG GGAGAGTGCA CTGCCAAATC GTCGGGGTAC AGACCGTGGA GAAGAAGGAG
    CCCTGGGCAA TAAAACGTGC CATCGAGAAA ACTCTGGAGA GAAATCTTCA ACTTCGACTG
    TCGAACCAAG ACTGGGCAAA GAAACTGGTG GGTTTCGGAA GCGATGGAGC TCCAGGTTCA
    GAGGGAGAAA ACGGCAGCAC AGTGGCCCTG CTTTTAAGGG AAATCCAGCC AGGTGTGCTG
    CCTGTTTACT GCCTTGCCCA TCACCTCGAA CTGTCTTACC AGGCGGTGTT CCAGAGCGTT
    CCCCTCTACA GCCGTGTGAA AGACCTTCTT CATAGCATGC ACCGCTTCTA TCACGATTCT
    CCTGCTCACA GGAGTGCTCT GGTGACTGCT TTCAAGGGCC TCCACCTTCA ACCTGCGATG
    CCCTCTCAAG TCAGAGGCCG GAGGTGGCTT CAGGGATTGC AGGCTGCCCT CCAGAACTTT
    CTCAAGGGAT ATCCAGCAAT TGTCCAGCAG CTGCAGTCAA CAGAAGAAAG CAGAAGCAAC
    AGCAGTCATC AGAAAGCCGA AGAACTCCTG GAGTCTCTCC TCCAGGCAGA CATTGTCAAA
    TTTGTCCACT TCTTGGTGGA TGTCGTCAAT GTCCTTAGCA TACTGTCCCA TGTCAATCAG
    AACAGAAATT CCTCAATTGC TGATGTATTT GCCACCTTAG AGTCAACACT GGAAATGCTC
    CAAATTTATC AGACAAGACC AGGGCCGAAA GAACGTGGGG TAGATTCGGT CACACACTTT
    CATGGTAACT GCCTTGGAGG AAAGGGAAAC ATTTCTGACG TGAGAAACAT TGTTTTAACA
    CATCTTATCA AGAGGCTGCG AGGCTGTTTC AGAGATGCCA ACCAAGATGT GGTGAAGGCA
    ACCACGGTTG GAAGCTTTAA ACTGTGGCCG GCAAAAATAA ATCAAGAATT TGGAGAAAAA
    GAGGTGGCCA TCCTGATTGC ACATTATGAA CCAGTATTAG AGGCAGCCAA GGTGAAAATT
    GACCAAGTAG ACACAGAATG GAGCATGTTG AAATTGGAGA TCTACTCTAG ATTTCAGAAC
    ATTTGCAAAC TGACCTGGGA TTTTGTGAAT TCTGTTTATT TACACAAGTA TCCAAACATC
    CTGACACTCA TTGACCTTGT GCTGAGCCTG CCTGCAAGTT CAGCTGAGGC AGGACGTGGC
    TTTAGTCAGA TGAAGCGCAT CAGGTCCCAA ATGCATGCCA AGGTCAAGGC TGACAACATG
    ACGGATCTCC TGATAATTCA GTTGAATTCT CCAGACATTA ACAACTTTGA CCCTAGGAAA
    GCCATTCATT TATGGAATAC AAGAACACCA TCCTCAACTG AGGACACAAG ACCTAACTCA
    GACTGCTCAT CAAACTCAGA AAGCCTTAGT GAAAGCGATA AGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_014961977.1
    CDS2303..8587
    Translation

    Target ORF information:

    RefSeq Version XM_015106491.1
    Organism Ovis aries musimon(Ovis orientalis musimon)
    Definition Ovis aries musimon sperm flagellar 2 (SPEF2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015106491.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGCAAGGTC CCAAGTCATT TGCAAAGGCA TTTTCCAGTG GCTATCTAAT TGGAGAAGTT 
    TTGCACAAGT TTGCACTTCA GGATGATTTT GCAGACTTTT CAGAAAGCAG GATTTCCAGT
    GCCAGACTTA ACAATTTTTC TCGCTTGGAG CCCACACTTC ACCTTTTGGG TGTGGAATTT
    GATCAGAATG TGGCCCACAA TATCATCACA GAGAAGCCTG GGGCTGCCAC GAAACTCTTA
    TATCAGTTGT ACATTGCTCT CCAGAGGAAA AAGAAAGGTG TTCAGACTAG AGTGGAAATG
    CCAACTGTAC AGCCCCTGAT GAGCACAAAA GTTCAAAACA TGAAAAGCGA GGCTTTCCGA
    GATCAACGCT TGAGCAAAAG ACGACAAAAT GAAATCATGG CCAAAATCCA AGCAGCTATC
    ATACAGATTC CTAAACCCAC ATCAAATCGT ACTCTGAAAA CATTTGACGC CCAAAAATTA
    ATGAAAAAGA AAAAAGAGGC AGAGGATGTG GCCAATGAAA TTAAGAAGTT TGAAGCATTA
    ATAAAAAAGG ATCTCCAGGC AAAAGAAAGT GCATCCAAGA CTTCTCTAGA TACAGCAGGC
    CAGACAACCG CTGATTTGTT AAACACTTAT TCAGATGATG ACTACATTAA AAAAATCCAA
    AAACGCCTTG AAGAAGATGC TTTTGCACGA GAGCAAAGGG AGAAAAGACG GCGGAGACTC
    TTAATGGATC AGTTAATAGC TCATGAAGCC CAAGAGGAGG CTTATCGAGA GGAGCAGTTG
    ATCAACCGGC TGATGCGACA GTCCCAGCAG GAACGCAGGA TCGCTGTGCA GCTCATGCAC
    GTTAGGCACG AAAAGGAAGT TTTATGGCAA AACAGAATTT TCAGAGAAAA ACAATATGAG
    GAAAGACGGC TTAAAGACTT CCAGGATGCT CTTGATCGAG AAGCGGCTTT AGCCAGACAA
    GCCAAGATTG ATTTTGAAGA ACAAGCCCTC AGAGATAAGG AAATTCATGA GCAGATCGCC
    ATAGAAAGAG CTCAAGCTCG TTACAAAAAG CATTATTCAA TATGTGCAGA AATTTTGGAT
    CAGATACTTG ATCTGTCCAC TAAGGTGGCA GACTATCGAT TGTTGACAAA TAACCTGATC
    CCATATAAGT TGATGCATGA TTGGAAGGAA CTGTTTTTCC ATGGAAAACC CATATATGAG
    CAAGCCTCTC TTAGACATTT ACCAGCTAAG CTCTCTGCAG AACACCTTGT AGAAATGGAG
    AAAAGGAACT TGCTAGATAA CAATGATTAT GAAGAATATA AGAACATGGT TGGAGAATGG
    GCCTTACCAG AAGAAATGGT TGAAAACTTA CCACCCTCCA ACAATTGCAT ATTGGGCCAT
    GTGATTCACA GACTAATTGA AAGTTCTCTT CCTCCTCAAG TTGAATCTAC AGTACCTGAG
    TTACCTTCAT TTGCTATAAA AGGATGCTTG TTGGGGAAAA CCTTTAGTGG AAAAACTACT
    GCTCTAAAGG CTCTAGAGAA AAGCTTTCCT ATTCAGGTAC TTTCTATTGA CACTCTTGTT
    CAAGAAGCTA TCCAAGCATT TCATGACAAT GAAAGTATCA GCGGGGCCCA TGGACTTCAG
    AAAGCACCTG AAGAAAAGGT TTTACCAGTT AGGCGAAAGA GTGGTAAAGA AAACCAGGAC
    GTGCAAGATG AAGCACTACC AGAAAGAGAA GATAAAACCA GACTTGATAC CGATGCTAGA
    AAAGCAGCAA AAGCTGAAGA AGTCGTAGCG AGTGATAGTT TCTCTGAACT CACTGTGCGT
    GCTCAGCTTG GTGCAAAATC AGAAATGCTG CTGAAGAAAG GAAAGAGCAT ATCTGATATA
    TTGCTAGTCA GCATCATGGT AAATGCCATT AATCAGATAC CTATGTATAA AGGCTGGATC
    CTGGATGGCT TTCCAATGAC ATTAAACCAA GCAAAACTTC TGGAGGAAGC ACTCACAGGC
    TACAACAGAA ACCTCATAGA ACTGGAGGGA AAGAAATCAC AAACATCCAC ATTAGCTGTT
    GACCCTACAG CTTCCAAAGA AGTGCCTCTT CCCCCTTCTG CATTTGATTT TGTCATGTTA
    TTAGACATTT CAGATAATTC CTCACTGAAT CGCATGAATG ACATCATGGC TAAAGCATTT
    TCAAGTGAAG CTCCTCGTGA AGATATCAAT CAGCGTGTAG CTGCTAAAAA CAAAGATACA
    GATGAGGACC AGAATTTAAG AGATCAAATA CAGCACAGAA TTGTAGGCTT CCTGGACAAC
    TGGCCTTCGT TGGAGCAGTG GTTTACAGAG CCAGAAAACA TTTTGATAAA AATCAATGCT
    GAAGTAGATA AACAGTCATT GTTCCAAAAA GTAAAGGAAA TGATTATGAC TGAAATAATT
    AAAAAAGAAA ATAAAGTGAA AAAGAAATTA GAAGAAAAGG AAGCTGAGAG AAAAGAAGAA
    GTTTCCCTGA GTGAGAAGAC ATCCCCTCCT CCTCCTCCCC CTCCGGAACC AGAGAAAGAG
    AAGGAAGTCC ACCTTCCACA GGAGCGGTCC AAAACTCCTG CTGCAAAAGG AAAGCAAGCA
    TCAGAACCCC TGCATGGTAA TCGAGAACCT CTTCAGGAAG GAAAAGGGAA GAAAAGTGAA
    ACTTCACTCA AAAGAAAAGG TTCTCCTAGA GGAAAATCGC TAGGAGGGAA GATTCCACTC
    AAGAAATCAC CTGCCGAGTC TACAGATATT TCACCGGTCC CGGTAGTGCC AGCCCCATCG
    AAGCCCGGAT CAGAAGAGTG GGTGTATGTA AATGAACCAA TTCCTGAGGA AATACCATCA
    TTTTTAGTAC CATACTGGAA ATTGATAGAA AATTCCTATA TAAATTCCAT CAAAACAGTA
    CTCAGGGAGT TGAGAGAAGG CCAGCATAGT GTACTTGCTT ACTTATATGA TATTAGAACA
    GGCTTCCAGC AGTTTCTGAA GCGTCCAGAC CACAAGCAAG ACTTCGTGTC TCAGTGGCAG
    GCGGATTTCA ACTCCCTTCC TGATGACCTG TGGGATGATG AGGAAACAAA GGCTGAGCTG
    CACCAAAGAG TGAATGATTT ACGGGACCGC CTGTGGGACA TTTGCGATGC CCGTAAGGAA
    GAAGCAGAGC AGGAGCGTCT GGACATCATT AACGAGAGCT GGTTGCAGGA CTCAATGGGA
    ATTGCCATGA ACCATTTCTT CTCACTGATG CAGGCAGAAC TGAACCGTTT CCAAGATACG
    AAGAGATTCC TGCAAGATTA TTACAGGGCC ATGGAATGTA AAATTCCGAT GGATGACACT
    AAGAAATTCA CTCGAGTCCC ACTGGTCCGA CTGGATAGTA GAGATATTTT GGAAAGCCAG
    CTTAGAATTC CCCTGGTTCC TCGAAGATCC ATTTCTCCAG AGTCAGCTTT ATCCAAACCA
    AAAACAAAAA GCATCCTGAA GGGCAAGATC GATTACACGC TGGAAAGTGT AGAGTTAAAC
    TTTGAGACTG ATGAGAAGAT GGTGATGGAT ACGTGGCAGC AGTCTTCTTT GGCAGTATCT
    CACATGGTGG CTGCTGAAAT TCATCAGAGG CTCATGGAAG AAGAAAAAGA AAACCAGCCA
    GCAGAAACAA AAGAAAAACT TCCTCAGGCA GCTGCAAATA AAAAAGTGAA AAAGGAGCCT
    CCGCCACCGC CACCACCACC CAAGAAAAAG AAAGAGGACA AAAAAGGAAA AGGTAAATCC
    CCACCTATGG CAGAAGCTAC TCCGGTAGTA GTGCCAGCAG AAGAGACTGA GGAAGTGGAA
    TTAAAAAATG AACTGAAGCT CAGAATAAAG GAAGAGCATC TTGCTGCCCT GCAATTTGAA
    GAGATAGCTA CACAGTTCCG ACTTGAACTG ATAAAGGTAA AAGCATTGGC TTTCCTGGAA
    GAGTTAGTAA TAAAGGCGGT TGATGTATAT AGACTCATGG ACAAGTGGCT CGGTGAGAGG
    TACTTGAATG AAATGGCCAG TGTGGAAAAA CTAACTGGGG TAGCTCGCTA TCACATTGAA
    ACATCAACCA AAATTCAGAA TGAACTTTAT TTAGACCAAG AAGACTTCTT CATTAATGGC
    GATATCAAAG TCTTTCCAGA TCCACCTCCT CCGGTACGCC CTCCGCCTGT GGAAAAGGAG
    GAAAATGGCA CCCTGACCAT GGAGCAGCTT GACAACCTCC GGGATCAGTT CTTAGATATA
    GCGCCCAAAG GCAGGGATCC TCCAGACAAT GTGAGTGGAT TCACCAAGAA GCAGAAAAGG
    CCTCGGAAGC TTGACCATTA CCTCCCAAAG AGGAACAATC AATTTTTGAA AATTTGGCTT
    AAAAAATACC CTTGGCTTGT ATACGATGAG ATACTAAATC TTATGTTCTG CGGCCTGTGT
    CGTAAACATG GAGTGACATT AAAGGGAAAT CAGGTCAGTT TTTTTTATGG CACGGATAAC
    TTCAGGACTG AATTTCTCAA CGCGCATCAT CTGAGTGAGG CTCACGCCAA GGCGTCCCTC
    ATGGAAGCCA CGAGTGGTTC TCCAGGGAGC AGAGCTACCA CGGAGCTGAT GGTGAGGACC
    ATGAGCAAGG TGACCCTTGG CAGGGTGGAG AACCTGTTCC GGTCCTGCCA CGCCATCGCC
    AAGACCGGAC GTCCCCTCAA AGACTTCCTC TGGATGTGTT CGCTGGACGA TATGAAGGGA
    GTTGATATCG GCCCGGTGTT CAGAACCAGT AAATCGGCCA GAACTTTTAC CTACTTTATT
    GCTGAAGTGG AACGGAGAAC TCTAAGAGAG AAGTTAGAAA AAAGTCAGTT CTTCTCCGTC
    CTCAGTGATG GGATTGCAGA CAGTTCAGCT GAGGAAGCTG AACTCGTATA TGTGCAGTTT
    GCCCGCGCAG GGAGAGTGCA CTGCCAAATC GTCGGGGTAC AGACCGTGGA GAAGAAGGAG
    CCCTGGGCAA TAAAACGTGC CATCGAGAAA ACTCTGGAGA GAAATCTTCA ACTTCGACTG
    TCGAACCAAG ACTGGGCAAA GAAACTGGTG GGTTTCGGAA GCGATGGAGC TCCAGGTTCA
    GAGGGAGAAA ACGGCAGCAC AGTGGCCCTG CTTTTAAGGG AAATCCAGCC AGGTGTGCTG
    CCTGTTTACT GCCTTGCCCA TCACCTCGAA CTGTCTTACC AGGCGGTGTT CCAGAGCGTT
    CCCCTCTACA GCCGTGTGAA AGACCTTCTT CATAGCATGC ACCGCTTCTA TCACGATTCT
    CCTGCTCACA GGAGTGCTCT GGTGACTGCT TTCAAGGGCC TCCACCTTCA ACCTGCGATG
    CCCTCTCAAG TCAGAGGCCG GAGGTGGCTT CAGGGATTGC AGGCTGCCCT CCAGAACTTT
    CTCAAGGGAT ATCCAGCAAT TGTCCAGCAG CTGCAGTCAA CAGAAGAAAG CAGAAGCAAC
    AGCAGTCATC AGAAAGCCGA AGAACTCCTG GAGTCTCTCC TCCAGGCAGA CATTGTCAAA
    TTTGTCCACT TCTTGGTGGA TGTCGTCAAT GTCCTTAGCA TACTGTCCCA TGTCAATCAG
    AACAGAAATT CCTCAATTGC TGATGTATTT GCCACCTTAG AGTCAACACT GGAAATGCTC
    CAAATTTATC AGACAAGACC AGGGCCGAAA GAACGTGGGG TAGATTCGGT CACACACTTT
    CATGGTAACT GCCTTGGAGG AAAGGGAAAC ATTTCTGACG TGAGAAACAT TGTTTTAACA
    CATCTTATCA AGAGGCTGCG AGGCTGTTTC AGAGATGCCA ACCAAGATGT GGTGAAGGCA
    ACCACGGTTG GAAGCTTTAA ACTGTGGCCG GCAAAAATAA ATCAAGAATT TGGAGAAAAA
    GAGGTGGCCA TCCTGATTGC ACATTATGAA CCAGTATTAG AGGCAGCCAA GGTGAAAATT
    GACCAAGTAG ACACAGAATG GAGCATGTTG AAATTGGAGA TCTACTCTAG ATTTCAGAAC
    ATTTGCAAAC TGACCTGGGA TTTTGTGAAT TCTGTTTATT TACACAAGTA TCCAAACATC
    CTGACACTCA TTGACCTTGT GCTGAGCCTG CCTGCAAGTT CAGCTGAGGC AGGACGTGGC
    TTTAGTCAGA TGAAGCGCAT CAGGTCCCAA ATGCATGCCA AGGTCAAGGC TGACAACATG
    ACGGATCTCC TGATAATTCA GTTGAATTCT CCAGACATTA ACAACTTTGA CCCTAGGAAA
    GCCATTCATT TATGGAATAC AAGAACACCA TCCTCAACTG AGGACACAAG ACCTAACTCA
    GACTGCTCAT CAAACTCAGA AAGCCTTAGT GAAAGCGATA AGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OOv13486
    Clone ID Related Accession (Same CDS sequence) XM_012129853.2
    Accession Version XM_012129853.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6159bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-16
    Organism Ovis aries musimon(Ovis orientalis musimon)
    Product sperm flagellar protein 2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942355.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012129853.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGTCGGAAA TCCTGTGCCA GTGGCTCAAC CAGGAATTGA AGGTGTCCCA GACCGTGAGT 
    CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAA TTGGAGAAGT TTTGCACAAG
    TTTGCACTTC AGGATGATTT TGCAGACTTT TCAGAAAGCA GGATTTCCAG TGCCAGACTT
    AACAATTTTT CTCGCTTGGA GCCCACACTT CACCTTTTGG GTGTGGAATT TGATCAGAAT
    GTGGCCCACA ATATCATCAC AGAGAAGCCT GGGGCTGCCA CGAAACTCTT ATATCAGTTG
    TACATTGCTC TCCAGAGGAA AAAGAAAGGT GTTCAGACTA GAGTGGAAAT GCCAACTGTA
    CAGCCCCTGA TGAGCACAAA AGTTCAAAAC ATGAAAAGCG AGGCTTTCCG AGATCAACGC
    TTGAGCAAAA GACGACAAAA TGAAATCATG GCCAAAATCC AAGCAGCTAT CATACAGATT
    CCTAAACCCA CATCAAATCG TACTCTGAAA ACATTTGACG CCCAAAAATT AATGAAAAAG
    AAAAAAGAGG CAGAGGATGT GGCCAATGAA ATTAAGAAGT TTGAAGCATT AATAAAAAAG
    GATCTCCAGG CAAAAGAAAG TGCATCCAAG ACTTCTCTAG ATACAGCAGG CCAGACAACC
    GCTGATTTGT TAAACACTTA TTCAGATGAT GACTACATTA AAAAAATCCA AAAACGCCTT
    GAAGAAGATG CTTTTGCACG AGAGCAAAGG GAGAAAAGAC GGCGGAGACT CTTAATGGAT
    CAGTTAATAG CTCATGAAGC CCAAGAGGAG GCTTATCGAG AGGAGCAGTT GATCAACCGG
    CTGATGCGAC AGTCCCAGCA GGAACGCAGG ATCGCTGTGC AGCTCATGCA CGTTAGGCAC
    GAAAAGGAAG TTTTATGGCA AAACAGAATT TTCAGAGAAA AACAATATGA GGAAAGACGG
    CTTAAAGACT TCCAGGATGC TCTTGATCGA GAAGCGGCTT TAGCCAGACA AGCCAAGATT
    GATTTTGAAG AACAAGCCCT CAGAGATAAG GAAATTCATG AGCAGATCGC CATAGAAAGA
    GCTCAAGCTC GTTACAAAAA GCATTATTCA ATATGTGCAG AAATTTTGGA TCAGATACTT
    GATCTGTCCA CTAAGGTGGC AGACTATCGA TTGTTGACAA ATAACCTGAT CCCATATAAG
    TTGATGCATG ATTGGAAGGA ACTGTTTTTC CATGGAAAAC CCATATATGA GCAAGCCTCT
    CTTAGACATT TACCAGCTAA GCTCTCTGCA GAACACCTTG TAGAAATGGA GAAAAGGAAC
    TTGCTAGATA ACAATGATTA TGAAGAATAT AAGAACATGG TTGGAGAATG GGCCTTACCA
    GAAGAAATGG TTGAAAACTT ACCACCCTCC AACAATTGCA TATTGGGCCA TGTGATTCAC
    AGACTAATTG AAAGTTCTCT TCCTCCTCAA GTTGAATCTA CAGTACCTGA GTTACCTTCA
    TTTGCTATAA AAGGATGCTT GTTGGGGAAA ACCTTTAGTG GAAAAACTAC TGCTCTAAAG
    GCTCTAGAGA AAAGCTTTCC TATTCAGGTA CTTTCTATTG ACACTCTTGT TCAAGAAGCT
    ATCCAAGCAT TTCATGACAA TGAAAGTATC AGCGGGGCCC ATGGACTTCA GAAAGCACCT
    GAAGAAAAGG TTTTACCAGT TAGGCGAAAG AGTGGTAAAG AAAACCAGGA CGTGCAAGAT
    GAAGCACTAC CAGAAAGAGA AGATAAAACC AGACTTGATA CCGATGCTAG AAAAGCAGCA
    AAAGCTGAAG AAGTCGTAGC GAGTGATAGT TTCTCTGAAC TCACTGTGCG TGCTCAGCTT
    GGTGCAAAAT CAGAAATGCT GCTGAAGAAA GGAAAGAGCA TATCTGATAT ATTGCTAGTC
    AGCATCATGG TAAATGCCAT TAATCAGATA CCTATGTATA AAGGCTGGAT CCTGGATGGC
    TTTCCAATGA CATTAAACCA AGCAAAACTT CTGGAGGAAG CACTCACAGG CTACAACAGA
    AACCTCATAG AACTGGAGGG AAAGAAATCA CAAACATCCA CATTAGCTGT TGACCCTACA
    GCTTCCAAAG AAGTGCCTCT TCCCCCTTCT GCATTTGATT TTGTCATGTT ATTAGACATT
    TCAGATAATT CCTCACTGAA TCGCATGAAT GACATCATGG CTAAAGCATT TTCAAGTGAA
    GCTCCTCGTG AAGATATCAA TCAGCGTGTA GCTGCTAAAA ACAAAGATAC AGATGAGGAC
    CAGAATTTAA GAGATCAAAT ACAGCACAGA ATTGTAGGCT TCCTGGACAA CTGGCCTTCG
    TTGGAGCAGT GGTTTACAGA GCCAGAAAAC ATTTTGATAA AAATCAATGC TGAAGTAGAT
    AAACAGTCAT TGTTCCAAAA AGTAAAGGAA ATGATTATGA CTGAAATAAT TAAAAAAGAA
    AATAAAGTGA AAAAGAAATT AGAAGAAAAG GAAGCTGAGA GAAAAGAAGA AGTTTCCCTG
    AGTGAGAAGA CATCCCCTCC TCCTCCTCCC CCTCCGGAAC CAGAGAAAGA GAAGGAAGTC
    CACCTTCCAC AGGAGCGGTC CAAAACTCCT GCTGCAAAAG GAAAGCAAGC ATCAGAACCC
    CTGCATGGTA ATCGAGAACC TCTTCAGGAA GGAAAAGGGA AGAAAAGTGA AACTTCACTC
    AAAAGAAAAG GTTCTCCTAG AGGAAAATCG CTAGGAGGGA AGATTCCACT CAAGAAATCA
    CCTGCCGAGT CTACAGATAT TTCACCGGTC CCGGTAGTGC CAGCCCCATC GAAGCCCGGA
    TCAGAAGAGT GGGTGTATGT AAATGAACCA ATTCCTGAGG AAATACCATC ATTTTTAGTA
    CCATACTGGA AATTGATAGA AAATTCCTAT ATAAATTCCA TCAAAACAGT ACTCAGGGAG
    TTGAGAGAAG GCCAGCATAG TGTACTTGCT TACTTATATG ATATTAGAAC AGGCTTCCAG
    CAGTTTCTGA AGCGTCCAGA CCACAAGCAA GACTTCGTGT CTCAGTGGCA GGCGGATTTC
    AACTCCCTTC CTGATGACCT GTGGGATGAT GAGGAAACAA AGGCTGAGCT GCACCAAAGA
    GTGAATGATT TACGGGACCG CCTGTGGGAC ATTTGCGATG CCCGTAAGGA AGAAGCAGAG
    CAGGAGCGTC TGGACATCAT TAACGAGAGC TGGTTGCAGG ACTCAATGGG AATTGCCATG
    AACCATTTCT TCTCACTGAT GCAGGCAGAA CTGAACCGTT TCCAAGATAC GAAGAGATTC
    CTGCAAGATT ATTACAGGGC CATGGAATGT AAAATTCCGA TGGATGACAC TAAGAAATTC
    ACTCGAGTCC CACTGGTCCG ACTGGATAGT AGAGATATTT TGGAAAGCCA GCTTAGAATT
    CCCCTGGTTC CTCGAAGATC CATTTCTCCA GAGTCAGCTT TATCCAAACC AAAAACAAAA
    AGCATCCTGA AGGGCAAGAT CGATTACACG CTGGAAAGTG TAGAGTTAAA CTTTGAGACT
    GATGAGAAGA TGGTGATGGA TACGTGGCAG CAGTCTTCTT TGGCAGTATC TCACATGGTG
    GCTGCTGAAA TTCATCAGAG GCTCATGGAA GAAGAAAAAG AAAACCAGCC AGCAGAAACA
    AAAGAAAAAC TTCCTCAGGC AGCTGCAAAT AAAAAAGTGA AAAAGGAGCC TCCGCCACCG
    CCACCACCAC CCAAGAAAAA GAAAGAGGAC AAAAAAGGAA AAGGTAAATC CCCACCTATG
    GCAGAAGCTA CTCCGGTAGT AGTGCCAGCA GAAGAGACTG AGGAAGTGGA ATTAAAAAAT
    GAACTGAAGC TCAGAATAAA GGAAGAGCAT CTTGCTGCCC TGCAATTTGA AGAGATAGCT
    ACACAGTTCC GACTTGAACT GATAAAGGTA AAAGCATTGG CTTTCCTGGA AGAGTTAGTA
    ATAAAGGCGG TTGATGTATA TAGACTCATG GACAAGTGGC TCGGTGAGAG GTACTTGAAT
    GAAATGGCCA GTGTGGAAAA ACTAACTGGG GTAGCTCGCT ATCACATTGA AACATCAACC
    AAAATTCAGA ATGAACTTTA TTTAGACCAA GAAGACTTCT TCATTAATGG CGATATCAAA
    GTCTTTCCAG ATCCACCTCC TCCGGTACGC CCTCCGCCTG TGGAAAAGGA GGAAAATGGC
    ACCCTGACCA TGGAGCAGCT TGACAACCTC CGGGATCAGT TCTTAGATAT AGCGCCCAAA
    GGCAGGGATC CTCCAGACAA TGTGAGTGGA TTCACCAAGA AGCAGAAAAG GCCTCGGAAG
    CTTGACCATT ACCTCCCAAA GAGGAACAAT CAATTTTTGA AAATTTGGCT TAAAAAATAC
    CCTTGGCTTG TATACGATGA GATACTAAAT CTTATGTTCT GCGGCCTGTG TCGTAAACAT
    GGAGTGACAT TAAAGGGAAA TCAGGTCAGT TTTTTTTATG GCACGGATAA CTTCAGGACT
    GAATTTCTCA ACGCGCATCA TCTGAGTGAG GCTCACGCCA AGGCGTCCCT CATGGAAGCC
    ACGAGTGGTT CTCCAGGGAG CAGAGCTACC ACGGAGCTGA TGGTGAGGAC CATGAGCAAG
    GTGACCCTTG GCAGGGTGGA GAACCTGTTC CGGTCCTGCC ACGCCATCGC CAAGACCGGA
    CGTCCCCTCA AAGACTTCCT CTGGATGTGT TCGCTGGACG ATATGAAGGG AGTTGATATC
    GGCCCGGTGT TCAGAACCAG TAAATCGGCC AGAACTTTTA CCTACTTTAT TGCTGAAGTG
    GAACGGAGAA CTCTAAGAGA GAAGTTAGAA AAAAGTCAGT TCTTCTCCGT CCTCAGTGAT
    GGGATTGCAG ACAGTTCAGC TGAGGAAGCT GAACTCGTAT ATGTGCAGTT TGCCCGCGCA
    GGGAGAGTGC ACTGCCAAAT CGTCGGGGTA CAGACCGTGG AGAAGAAGGA GCCCTGGGCA
    ATAAAACGTG CCATCGAGAA AACTCTGGAG AGAAATCTTC AACTTCGACT GTCGAACCAA
    GACTGGGCAA AGAAACTGGT GGGTTTCGGA AGCGATGGAG CTCCAGGTTC AGAGGGAGAA
    AACGGCAGCA CAGTGGCCCT GCTTTTAAGG GAAATCCAGC CAGGTGTGCT GCCTGTTTAC
    TGCCTTGCCC ATCACCTCGA ACTGTCTTAC CAGGCGGTGT TCCAGAGCGT TCCCCTCTAC
    AGCCGTGTGA AAGACCTTCT TCATAGCATG CACCGCTTCT ATCACGATTC TCCTGCTCAC
    AGGAGTGCTC TGGTGACTGC TTTCAAGGGC CTCCACCTTC AACCTGCGAT GCCCTCTCAA
    GTCAGAGGCC GGAGGTGGCT TCAGGGATTG CAGGCTGCCC TCCAGAACTT TCTCAAGGGA
    TATCCAGCAA TTGTCCAGCA GCTGCAGTCA ACAGAAGAAA GCAGAAGCAA CAGCAGTCAT
    CAGAAAGCCG AAGAACTCCT GGAGTCTCTC CTCCAGGCAG ACATTGTCAA ATTTGTCCAC
    TTCTTGGTGG ATGTCGTCAA TGTCCTTAGC ATACTGTCCC ATGTCAATCA GAACAGAAAT
    TCCTCAATTG CTGATGTATT TGCCACCTTA GAGTCAACAC TGGAAATGCT CCAAATTTAT
    CAGACAAGAC CAGGGCCGAA AGAACGTGGG GTAGATTCGG TCACACACTT TCATGGTAAC
    TGCCTTGGAG GAAAGGGAAA CATTTCTGAC GTGAGAAACA TTGTTTTAAC ACATCTTATC
    AAGAGGCTGC GAGGCTGTTT CAGAGATGCC AACCAAGATG TGGTGAAGGC AACCACGGTT
    GGAAGCTTTA AACTGTGGCC GGCAAAAATA AATCAAGAAT TTGGAGAAAA AGAGGTGGCC
    ATCCTGATTG CACATTATGA ACCAGTATTA GAGGCAGCCA AGGTGAAAAT TGACCAAGTA
    GACACAGAAT GGAGCATGTT GAAATTGGAG ATCTACTCTA GATTTCAGAA CATTTGCAAA
    CTGACCTGGG ATTTTGTGAA TTCTGTTTAT TTACACAAGT ATCCAAACAT CCTGACACTC
    ATTGACCTTG TGCTGAGCCT GCCTGCAAGT TCAGCTGAGG CAGGACGTGG CTTTAGTCAG
    ATGAAGCGCA TCAGGTCCCA AATGCATGCC AAGGTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011985243.1
    CDS497..6655
    Translation

    Target ORF information:

    RefSeq Version XM_012129853.2
    Organism Ovis aries musimon(Ovis orientalis musimon)
    Definition Ovis aries musimon sperm flagellar 2 (SPEF2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012129853.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGTCGGAAA TCCTGTGCCA GTGGCTCAAC CAGGAATTGA AGGTGTCCCA GACCGTGAGT 
    CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAA TTGGAGAAGT TTTGCACAAG
    TTTGCACTTC AGGATGATTT TGCAGACTTT TCAGAAAGCA GGATTTCCAG TGCCAGACTT
    AACAATTTTT CTCGCTTGGA GCCCACACTT CACCTTTTGG GTGTGGAATT TGATCAGAAT
    GTGGCCCACA ATATCATCAC AGAGAAGCCT GGGGCTGCCA CGAAACTCTT ATATCAGTTG
    TACATTGCTC TCCAGAGGAA AAAGAAAGGT GTTCAGACTA GAGTGGAAAT GCCAACTGTA
    CAGCCCCTGA TGAGCACAAA AGTTCAAAAC ATGAAAAGCG AGGCTTTCCG AGATCAACGC
    TTGAGCAAAA GACGACAAAA TGAAATCATG GCCAAAATCC AAGCAGCTAT CATACAGATT
    CCTAAACCCA CATCAAATCG TACTCTGAAA ACATTTGACG CCCAAAAATT AATGAAAAAG
    AAAAAAGAGG CAGAGGATGT GGCCAATGAA ATTAAGAAGT TTGAAGCATT AATAAAAAAG
    GATCTCCAGG CAAAAGAAAG TGCATCCAAG ACTTCTCTAG ATACAGCAGG CCAGACAACC
    GCTGATTTGT TAAACACTTA TTCAGATGAT GACTACATTA AAAAAATCCA AAAACGCCTT
    GAAGAAGATG CTTTTGCACG AGAGCAAAGG GAGAAAAGAC GGCGGAGACT CTTAATGGAT
    CAGTTAATAG CTCATGAAGC CCAAGAGGAG GCTTATCGAG AGGAGCAGTT GATCAACCGG
    CTGATGCGAC AGTCCCAGCA GGAACGCAGG ATCGCTGTGC AGCTCATGCA CGTTAGGCAC
    GAAAAGGAAG TTTTATGGCA AAACAGAATT TTCAGAGAAA AACAATATGA GGAAAGACGG
    CTTAAAGACT TCCAGGATGC TCTTGATCGA GAAGCGGCTT TAGCCAGACA AGCCAAGATT
    GATTTTGAAG AACAAGCCCT CAGAGATAAG GAAATTCATG AGCAGATCGC CATAGAAAGA
    GCTCAAGCTC GTTACAAAAA GCATTATTCA ATATGTGCAG AAATTTTGGA TCAGATACTT
    GATCTGTCCA CTAAGGTGGC AGACTATCGA TTGTTGACAA ATAACCTGAT CCCATATAAG
    TTGATGCATG ATTGGAAGGA ACTGTTTTTC CATGGAAAAC CCATATATGA GCAAGCCTCT
    CTTAGACATT TACCAGCTAA GCTCTCTGCA GAACACCTTG TAGAAATGGA GAAAAGGAAC
    TTGCTAGATA ACAATGATTA TGAAGAATAT AAGAACATGG TTGGAGAATG GGCCTTACCA
    GAAGAAATGG TTGAAAACTT ACCACCCTCC AACAATTGCA TATTGGGCCA TGTGATTCAC
    AGACTAATTG AAAGTTCTCT TCCTCCTCAA GTTGAATCTA CAGTACCTGA GTTACCTTCA
    TTTGCTATAA AAGGATGCTT GTTGGGGAAA ACCTTTAGTG GAAAAACTAC TGCTCTAAAG
    GCTCTAGAGA AAAGCTTTCC TATTCAGGTA CTTTCTATTG ACACTCTTGT TCAAGAAGCT
    ATCCAAGCAT TTCATGACAA TGAAAGTATC AGCGGGGCCC ATGGACTTCA GAAAGCACCT
    GAAGAAAAGG TTTTACCAGT TAGGCGAAAG AGTGGTAAAG AAAACCAGGA CGTGCAAGAT
    GAAGCACTAC CAGAAAGAGA AGATAAAACC AGACTTGATA CCGATGCTAG AAAAGCAGCA
    AAAGCTGAAG AAGTCGTAGC GAGTGATAGT TTCTCTGAAC TCACTGTGCG TGCTCAGCTT
    GGTGCAAAAT CAGAAATGCT GCTGAAGAAA GGAAAGAGCA TATCTGATAT ATTGCTAGTC
    AGCATCATGG TAAATGCCAT TAATCAGATA CCTATGTATA AAGGCTGGAT CCTGGATGGC
    TTTCCAATGA CATTAAACCA AGCAAAACTT CTGGAGGAAG CACTCACAGG CTACAACAGA
    AACCTCATAG AACTGGAGGG AAAGAAATCA CAAACATCCA CATTAGCTGT TGACCCTACA
    GCTTCCAAAG AAGTGCCTCT TCCCCCTTCT GCATTTGATT TTGTCATGTT ATTAGACATT
    TCAGATAATT CCTCACTGAA TCGCATGAAT GACATCATGG CTAAAGCATT TTCAAGTGAA
    GCTCCTCGTG AAGATATCAA TCAGCGTGTA GCTGCTAAAA ACAAAGATAC AGATGAGGAC
    CAGAATTTAA GAGATCAAAT ACAGCACAGA ATTGTAGGCT TCCTGGACAA CTGGCCTTCG
    TTGGAGCAGT GGTTTACAGA GCCAGAAAAC ATTTTGATAA AAATCAATGC TGAAGTAGAT
    AAACAGTCAT TGTTCCAAAA AGTAAAGGAA ATGATTATGA CTGAAATAAT TAAAAAAGAA
    AATAAAGTGA AAAAGAAATT AGAAGAAAAG GAAGCTGAGA GAAAAGAAGA AGTTTCCCTG
    AGTGAGAAGA CATCCCCTCC TCCTCCTCCC CCTCCGGAAC CAGAGAAAGA GAAGGAAGTC
    CACCTTCCAC AGGAGCGGTC CAAAACTCCT GCTGCAAAAG GAAAGCAAGC ATCAGAACCC
    CTGCATGGTA ATCGAGAACC TCTTCAGGAA GGAAAAGGGA AGAAAAGTGA AACTTCACTC
    AAAAGAAAAG GTTCTCCTAG AGGAAAATCG CTAGGAGGGA AGATTCCACT CAAGAAATCA
    CCTGCCGAGT CTACAGATAT TTCACCGGTC CCGGTAGTGC CAGCCCCATC GAAGCCCGGA
    TCAGAAGAGT GGGTGTATGT AAATGAACCA ATTCCTGAGG AAATACCATC ATTTTTAGTA
    CCATACTGGA AATTGATAGA AAATTCCTAT ATAAATTCCA TCAAAACAGT ACTCAGGGAG
    TTGAGAGAAG GCCAGCATAG TGTACTTGCT TACTTATATG ATATTAGAAC AGGCTTCCAG
    CAGTTTCTGA AGCGTCCAGA CCACAAGCAA GACTTCGTGT CTCAGTGGCA GGCGGATTTC
    AACTCCCTTC CTGATGACCT GTGGGATGAT GAGGAAACAA AGGCTGAGCT GCACCAAAGA
    GTGAATGATT TACGGGACCG CCTGTGGGAC ATTTGCGATG CCCGTAAGGA AGAAGCAGAG
    CAGGAGCGTC TGGACATCAT TAACGAGAGC TGGTTGCAGG ACTCAATGGG AATTGCCATG
    AACCATTTCT TCTCACTGAT GCAGGCAGAA CTGAACCGTT TCCAAGATAC GAAGAGATTC
    CTGCAAGATT ATTACAGGGC CATGGAATGT AAAATTCCGA TGGATGACAC TAAGAAATTC
    ACTCGAGTCC CACTGGTCCG ACTGGATAGT AGAGATATTT TGGAAAGCCA GCTTAGAATT
    CCCCTGGTTC CTCGAAGATC CATTTCTCCA GAGTCAGCTT TATCCAAACC AAAAACAAAA
    AGCATCCTGA AGGGCAAGAT CGATTACACG CTGGAAAGTG TAGAGTTAAA CTTTGAGACT
    GATGAGAAGA TGGTGATGGA TACGTGGCAG CAGTCTTCTT TGGCAGTATC TCACATGGTG
    GCTGCTGAAA TTCATCAGAG GCTCATGGAA GAAGAAAAAG AAAACCAGCC AGCAGAAACA
    AAAGAAAAAC TTCCTCAGGC AGCTGCAAAT AAAAAAGTGA AAAAGGAGCC TCCGCCACCG
    CCACCACCAC CCAAGAAAAA GAAAGAGGAC AAAAAAGGAA AAGGTAAATC CCCACCTATG
    GCAGAAGCTA CTCCGGTAGT AGTGCCAGCA GAAGAGACTG AGGAAGTGGA ATTAAAAAAT
    GAACTGAAGC TCAGAATAAA GGAAGAGCAT CTTGCTGCCC TGCAATTTGA AGAGATAGCT
    ACACAGTTCC GACTTGAACT GATAAAGGTA AAAGCATTGG CTTTCCTGGA AGAGTTAGTA
    ATAAAGGCGG TTGATGTATA TAGACTCATG GACAAGTGGC TCGGTGAGAG GTACTTGAAT
    GAAATGGCCA GTGTGGAAAA ACTAACTGGG GTAGCTCGCT ATCACATTGA AACATCAACC
    AAAATTCAGA ATGAACTTTA TTTAGACCAA GAAGACTTCT TCATTAATGG CGATATCAAA
    GTCTTTCCAG ATCCACCTCC TCCGGTACGC CCTCCGCCTG TGGAAAAGGA GGAAAATGGC
    ACCCTGACCA TGGAGCAGCT TGACAACCTC CGGGATCAGT TCTTAGATAT AGCGCCCAAA
    GGCAGGGATC CTCCAGACAA TGTGAGTGGA TTCACCAAGA AGCAGAAAAG GCCTCGGAAG
    CTTGACCATT ACCTCCCAAA GAGGAACAAT CAATTTTTGA AAATTTGGCT TAAAAAATAC
    CCTTGGCTTG TATACGATGA GATACTAAAT CTTATGTTCT GCGGCCTGTG TCGTAAACAT
    GGAGTGACAT TAAAGGGAAA TCAGGTCAGT TTTTTTTATG GCACGGATAA CTTCAGGACT
    GAATTTCTCA ACGCGCATCA TCTGAGTGAG GCTCACGCCA AGGCGTCCCT CATGGAAGCC
    ACGAGTGGTT CTCCAGGGAG CAGAGCTACC ACGGAGCTGA TGGTGAGGAC CATGAGCAAG
    GTGACCCTTG GCAGGGTGGA GAACCTGTTC CGGTCCTGCC ACGCCATCGC CAAGACCGGA
    CGTCCCCTCA AAGACTTCCT CTGGATGTGT TCGCTGGACG ATATGAAGGG AGTTGATATC
    GGCCCGGTGT TCAGAACCAG TAAATCGGCC AGAACTTTTA CCTACTTTAT TGCTGAAGTG
    GAACGGAGAA CTCTAAGAGA GAAGTTAGAA AAAAGTCAGT TCTTCTCCGT CCTCAGTGAT
    GGGATTGCAG ACAGTTCAGC TGAGGAAGCT GAACTCGTAT ATGTGCAGTT TGCCCGCGCA
    GGGAGAGTGC ACTGCCAAAT CGTCGGGGTA CAGACCGTGG AGAAGAAGGA GCCCTGGGCA
    ATAAAACGTG CCATCGAGAA AACTCTGGAG AGAAATCTTC AACTTCGACT GTCGAACCAA
    GACTGGGCAA AGAAACTGGT GGGTTTCGGA AGCGATGGAG CTCCAGGTTC AGAGGGAGAA
    AACGGCAGCA CAGTGGCCCT GCTTTTAAGG GAAATCCAGC CAGGTGTGCT GCCTGTTTAC
    TGCCTTGCCC ATCACCTCGA ACTGTCTTAC CAGGCGGTGT TCCAGAGCGT TCCCCTCTAC
    AGCCGTGTGA AAGACCTTCT TCATAGCATG CACCGCTTCT ATCACGATTC TCCTGCTCAC
    AGGAGTGCTC TGGTGACTGC TTTCAAGGGC CTCCACCTTC AACCTGCGAT GCCCTCTCAA
    GTCAGAGGCC GGAGGTGGCT TCAGGGATTG CAGGCTGCCC TCCAGAACTT TCTCAAGGGA
    TATCCAGCAA TTGTCCAGCA GCTGCAGTCA ACAGAAGAAA GCAGAAGCAA CAGCAGTCAT
    CAGAAAGCCG AAGAACTCCT GGAGTCTCTC CTCCAGGCAG ACATTGTCAA ATTTGTCCAC
    TTCTTGGTGG ATGTCGTCAA TGTCCTTAGC ATACTGTCCC ATGTCAATCA GAACAGAAAT
    TCCTCAATTG CTGATGTATT TGCCACCTTA GAGTCAACAC TGGAAATGCT CCAAATTTAT
    CAGACAAGAC CAGGGCCGAA AGAACGTGGG GTAGATTCGG TCACACACTT TCATGGTAAC
    TGCCTTGGAG GAAAGGGAAA CATTTCTGAC GTGAGAAACA TTGTTTTAAC ACATCTTATC
    AAGAGGCTGC GAGGCTGTTT CAGAGATGCC AACCAAGATG TGGTGAAGGC AACCACGGTT
    GGAAGCTTTA AACTGTGGCC GGCAAAAATA AATCAAGAAT TTGGAGAAAA AGAGGTGGCC
    ATCCTGATTG CACATTATGA ACCAGTATTA GAGGCAGCCA AGGTGAAAAT TGACCAAGTA
    GACACAGAAT GGAGCATGTT GAAATTGGAG ATCTACTCTA GATTTCAGAA CATTTGCAAA
    CTGACCTGGG ATTTTGTGAA TTCTGTTTAT TTACACAAGT ATCCAAACAT CCTGACACTC
    ATTGACCTTG TGCTGAGCCT GCCTGCAAGT TCAGCTGAGG CAGGACGTGG CTTTAGTCAG
    ATGAAGCGCA TCAGGTCCCA AATGCATGCC AAGGTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OSm32423
    Clone ID Related Accession (Same CDS sequence) XM_012097074.2
    Accession Version XM_012097074.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6336bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-16
    Organism Ovis aries(sheep)
    Product sperm flagellar protein 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014639025.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012097074.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGTCGGAAA TCCTGTGCCA GTGGCTCAAC CAGGAATTGA AGGTGTCCCA GACCGTGAGT 
    CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAA TTGGAGAAGT TTTGCACAAG
    TTTGCACTTC AGGATGATTT TGCAGACTTT TCAGAAAGCA GGATTTCCAG TGCCAGACTT
    AACAATTTTT CTCGCTTGGA GCCCACACTT CACCTTTTGG GTGTGCAATT TGATCAGAAT
    GTGGCCCACA ATATCATCAC AGAGAAGCCT GGGGCTGCCA CGAAACTCTT ATATCAGTTG
    TACATTGCTC TCCAGAGGAA AAAGAAAGGT GTTCAGACTA GAGTGGAAAT GCCAACTGTA
    CAGCCCCTGA TGAGCACAAA AGTTCAAAAC ATGAAAAGCG AGGCTTTCCG AGATCAACGC
    TTGAGCAAAA GACGACAAAA TGAAATCATG GCCAAAATCC AAGCAGCTAT CATACAGATT
    CCTAAACCCA CATCAAATCG TACTCTGAAA ACATTTGACG CCCAAAAATT AATGAAAAAG
    AAAAAAGAGG CAGAGGATGT GGCCAATGAA ATTAAGAAGT TTGAAGCATT AATAAAAAAG
    GATCTCCAGG CAAAAGAAAG TGCATCCAAG ACTTCTCTAG ATACAGCAGG CCAGACAACC
    GCTGATTTGT TAAACACTTA TTCAGATGAT GACTACATTA AAAAAATCCA AAAACGCCTT
    GAAGAAGATG CTTTTGCACG AGAGCAAAGG GAGAAAAGAC GGCGGAGACT CTTAATGGAT
    CAGTTAATAG CTCATGAAGC CCAAGAGGAG GCTTATCGGG AGGAGCAGTT GATCAACCGG
    CTGATGCGAC AGTCCCAGCA GGAACGCAGG ATCGCTGTGC AGCTCATGCA CGTTAGGCAT
    GAAAAGGAAG TTTTATGGCA AAACAGAATT TTCAGAGAAA AACAATATGA GGAAAGACGG
    CTTAAAGACT TCCAGGATGC TCTTGATCGA GAAGCGGCTT TAGCCAGACA AGCCAAGATT
    GATTTTGAAG AACAAGCCCT CAGAGATAAG GAAATTCATG AGCAGATCGC CATAGAAAGA
    GCTCAAGCTC GTTACAAAAA GCATTATTCA ATATGTGCAG AAATTTTGGA TCAGATACTT
    GATCTGTCCA CTAAGGTGGC AGACTATCGA TTGTTGACAA ATAACCTGAT CCCATATAAG
    TTGATGCATG ATTGGAAGGA ACTGTTTTTC CATGGAAAAC CCATATATGA GCAAGCCTCT
    CTTAGACATT TACCAGCTAA GCTCTCTGCA GAACACCTTG TAGAAATGGA GAAAAGGAAC
    TTGCTAGATA ACAATGATTA TGAAGAATAT AAGAACATGG TTGGAGAATG GGCCTTACCA
    GAAGAAATGG TTGAAAACTT ACCACCCTCC AACAATTGCA TATTGGGCCA TGTGATTCAC
    AGACTAATTG AAAGTTCTCT TCCTCCTCAA GTTGAATCTA CAGTACCTGA GTTACCTTCA
    TTTGCTATAA AAGGATGCTT GTTGGGGAAA ACCTTTAGTG GAAAAACTAC TGCTCTAAAG
    GCTCTAGAGA AAAGCTTTCC TATTCAGGTA CTTTCTATTG ACACTCTTGT TCAAGAAGCT
    ATCCAAGCAT TTCATGACAA TGAAAGTATC AGCGGGGCCC ATGGACTTCA GAAAGCACCT
    GAAGAAAAGG TTTTACCAGT TAGGCGAAAG AGTGGTAAAG AAAACCAGGA CGTGCAAGAT
    GAAGCACTAC CAGAAAGAGA AGATAAAACC AGACTTGATA CCGATGCTAG AAAAGCAGCA
    AAAGCTGAAG AAGTCGTAGC GAGGGATAGT TTCTCTGAAC TCACTGTGCG TGCTCAGCTT
    GGTGCAAAAT CAGAAATGCT GCTGAAGAAA GGAAAGAGCA TATCTGATAT ATTGCTAGTC
    AGCATCATGG TAAATGCCAT TAATCAGATA CCTATGTATA AAGGCTGGAT CCTGGATGGC
    TTTCCAATGA CATTAAACCA AGCAAAACTT CTGGAGGAAG CACTCACAGG CTACAACAGA
    AACCTCATAG AACTGGAGGG AAAGAAATCA CAAACATCCA CATTAGCTGT TGACCCTACA
    GCTTCCAAAG AAGTGCCTCT TCCCCCTTCT GCATTTGATT TTGTCATGTT ATTAGACATT
    TCAGATAATT CCTCACTGAA TCGCATGAAT GACATCATGG CTAAAGCATT TTCAAGTGAA
    GCTCCTCGTG AAGATATCAA TCAGCGTGTA GCTGCTAAAA ACAAAGATAC AGATGAGGAC
    CAGAATTTAA GAGATCAAAT ACAGCACAGA ATTGTAGGCT TCCTGGACAA CTGGCCTTCG
    TTGGAGCAGT GGTTTACAGA GCCAGAAAAC ATTTTGATAA AAATCAATGC TGAAGTAGAT
    AAACAGTCAT TGTTCCAAAA AGTAAAGGAA ATGATTATGA CTGAAATAAT TAAAAAAGAA
    AATAAAGTGA AAAAGAAATT AGAAGAAAAG GAAGCTGAGA GAAAAGAAGA AGTTTCCCTG
    AGTGAGAAGA CATCCCCTCC TCCTCCTCCC CCTCCGGAAC CAGAGAAAGA GAAGGAAGTC
    CACCTTCCAC AGGAGCGGTC CAAAACTCCT GCTGCAAAAG GAAAGCAAGC ATCAGAACCC
    CTGCATGGTA ATCGAGAACC TCTTCAGGAA GGAAAAGGGA AGAAAAGTGA AACTTCACTC
    AAAAGAAAAG GTTCTCCTAG AGGAAAATCG CTAGGAGGGA AGATTCCACT CAAGAAATCA
    CCTGCCGAGT CTACAGATAT TTCACCGGTC CCGGTAGTGC CAGCCCCATC GAAGCCCGGA
    TCAGAAGAGT GGGTGTATGT AAATGAACCA ATTCCTGAGG AAATACCATC ATTTTTAGTA
    CCATACTGGA AATTGATAGA AAACTCCTAT ATAAATTCCA TCAAAACAGT ACTCAGGGAG
    TTGAGAGAAG GCCAGCATAG TGTACTTGCT TACTTATATG ATATTAGAAC AGGCTTCCAG
    CAGTTTCTGA AGCGTCCAGA CCACAAGCAA GACTTCGTGT CTCAGTGGCA GGCGGATTTC
    AACTCCCTTC CTGATGACCT GTGGGATGAT GAGGAAACAA AGGCTGAGCT GCACCAAAGA
    GTGAATGATT TACGGGACCG CCTGTGGGAC ATTTGCGATG CCCGTAAGGA AGAAGCAGAG
    CAGGAGCGTC TGGACATCAT TAACGAGAGC TGGTTGCAGG ACTCAATGGG AATTGCCATG
    AACCATTTCT TCTCACTGAT GCAGGCAGAA CTGAACCGTT TCCAAGATAC GAAGAGATTC
    CTGCAAGATT ATTACAGGGC CATGGAATGT AAAATTCCGA TGGATGACAC TAAGAAATTC
    ACTCGAGTCC CACTGGTCCG ACTGGATAGT AGAGATATTT TGGAAAGCCA GCTTAGAATT
    CCCCTGGTTC CTCGAAGATC CATTTCTCCA GAGTCAGCTT TATCCAAACC AAAAACAAAA
    AGCATCCTGA AGGGCAAGAT CGATTACACG CTGGAAAGTG TAGAGTTAAA CTTTGAGACT
    GATGAGAAGA TGGTGATGGA TACGTGGCAG CAGTCTTCTT TGGCAGTATC TCACATGGTG
    GCTGCTGAAA TTCATCAGAG GCTCATGGAA GAAGAAAAAG AAAACCAGCC AGCAGAAACA
    AAAGAAAAAC TTCCTCAGGC AGCTGCAAAT AAAAAAGTGA AAAAGGAGCC TCCGCCACCG
    CCACCACCAC CCAAGAAAAA GAAAGAGGAC AAAAAAGGAA AAGGTAAATC CCCACCTATG
    GCAGAAGCTA CTCCTGTAGT AGTGCCAGCA GAAGAGACTG AGGAAGTGGA ATTAAAAAAT
    GAACTGAAGC TCAGAATAAA GGAAGAGCAT CTTGCTGCCC TGCAATTTGA AGAGATAGCT
    ACACAGTTCC GACTTGAACT GATAAAGGTA AAAGCATTGG CTTTCCTGGA AGAGTTAGTA
    ATAAAGGCGG TTGATGTATA TAGACTCATG GACAAGTGGC TCGGTGAGAG GTACTTGAAT
    GAAATGGCCA GTGTGGAAAA ACTAACTGGG GTAGCTCGCT ATCACATTGA AACATCAACC
    AAAATTCAGA ATGAACTTTA TTTAGACCAA GAAGACTTCT TCATTAATGG CGATATCAAA
    GTCTTTCCAG ATCCACCTCC TCCGGTACGC CCTCCGCCTG TGGAAAAGGA GGAAAATGGC
    ACCCTGACCA TGGAGCAGCT TGACAACCTC CGGGATCAGT TCTTAGATAT AGCGCCCAAA
    GGCAGGGATC CTCCAGACAA TGTGAGTGGA TTCACCAAGA AGCAGAAAAG GCCTCAGAAG
    CTTGACCATT ACCTCCCAAA GAGGAACAAT CAATTTTTGA AAATTTGGCT TAAAAAATAC
    CCTTGGCTTG TATACGATGA GATACTAAAT CTTATGTTCT GCGGCCTGTG TCGTAAACAT
    GGAGTGACAT CAAAGGGAAA TCAGGTCAGT TTTTTTTACG GCACGGATAA CTTCAGGACT
    GAATTTCTCA ACGCGCATCA TCTGAGTGAG GCTCACGCCA AGGCGTCCCT CATGGAAGCC
    ACAAGTGGTT CTCCAGGGAG CAGAGCTACC ACGGAGCTGA TGGTGAGGAC CATGAGCAAG
    GTGACCCTTG GCAGGGTGGA GAACCTGTTC CGGTCCTGCC ACGCCATCGC CAAGACCGGA
    CGTCCCCTCA AAGACTTCCT CTGGATGTGT TCGCTGGACG ATATGAAGGG AATTGATATC
    GGCCCGGTGT TCAGAACCAG TAAATCGGCC AGAACTTTTA CCTACTTTAT TGCTGAAGTG
    GAACGGAGAA CTCTAAGAGA GAAGTTAGAA AAAAGTCAGT TCTTCTCCGT CCTCAGTGAT
    GGGATTGCAG ACAATTCAGC TGAGGAAGCT GAACTCGTAT ATGTGCAGTT TGCCCGCGCA
    GGGAGAGTGC ACTGCCAAAT CGTCGGGGTA CAGACCGTGG AGAAGAAGGA GCCCTGGGCG
    ATAAAACGTG CCATTGAGAA AACTCTGGAG AGAAATCTTC AACTTCGACT GTCGAACCAA
    GACTGGGCAA AGAAACTGGT GGGTTTCGGA AGCGATGGAG CTCCAGGTTC AGAGGGAGAA
    AACGGCAGCA CAGTGGCCCT GCTTTTAAGG GAAATCCAGC CAGGTGTGCT GCCTGTTTAC
    TGCCTTGCCC ATCACCTCGA ACTGTCTTAC CAGGCGGTGT TCCAGAGCGT TCCCCTCTAC
    AGCCGTGTGA AAGACCTTCT TCATAGCATG CACCGCTTCT ATCACGATTC TCCTGCTCAC
    AGGAGTGCTC TGGTGACTGC TTTCAAGGGC CTCCACCTTC AACCTGCGAT GCCCTCTCAA
    GTCAGAGGCC GGAGGTGGCT TCAGGGATTG CAGGCTGCCC TCCAGAACTT TCTCAAGGGA
    TATCCAGCAA TTGTCCAGCA GCTGCAGTCA ACAAAAGAAA GCAGAAGCAA CAGCAGTCAT
    CAGAAAGCCG AAGAACTCCT GGAGTCTCTC CTCCAGGCAG ACATTGTCAA ATTTGTCCAC
    TTCTTGGTGG ATGTCGTCAA TGTCCTTAGC ATACTGTCCC ATGTCAATCA GAACAGAAAT
    TCCTCAATTG CTGATGTATT TGCCACCTTA GAGTCAACAC TGGAAATGCT CCAAATTTAT
    CAGACAAGAC CAGGGCCGAA AGAACGTGGG GTAGATTCGG TCACACACTT TCATGGTAAC
    TGCCTTGGAG GAAAGGGAAA CATTTCTGAC GTGAGAAACA TTGTTTTAAC ACATCTTATC
    AAGAGGCTGC GAGGCTGTTT CAGAGATGCC AACCAAGATG TGGTGAAGGC AACCACGATT
    GGAAGCTTTA AACTGTGGCC GGCAAAAATA AATCAAGAAT TTGGAGAAAA AGAGGTGGCC
    ATCCTGATTG CACATTATGA ACCAGTATTA GAGGCAGCCA AGGTGAAAAT TGACCAAGTA
    GACACAGAAT GGAGCATGTT GAAATTGGAG ATCTACTCTA GATTTCAGAA CATTTGCAAA
    CTGACCTGGG ATTTTGTGAA TTCTGTTTAT TTACACAAGT ATCCAAATAT CCTGACACTC
    ATTGACCTCG TGCTGAGCCT GCCTGCAAGT TCAGCTGAGG CAGGACGTGG CTTTAGTCAG
    ATGAAGCGCA TCAGGTCCCA AATGCATGCC AAGGTCAAGG CTGACAACAT GACGGATCTC
    CTGATAATTC AGTTGAATTC TCCAGACATT AACAACTTTG ACCCTAGGAA AGCCATTCAT
    TTATGGAATA CAAGAACACC ATCCTCAACT GAGGACACAA GACCTAACTC AGACTGCTCA
    TCAAACTCAG AAAGCCTTAG TGAAAGCGAT GAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011952464.1
    CDS497..6832
    Translation

    Target ORF information:

    RefSeq Version XM_012097074.2
    Organism Ovis aries(sheep)
    Definition Ovis aries sperm flagellar 2 (SPEF2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012097074.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGTCGGAAA TCCTGTGCCA GTGGCTCAAC CAGGAATTGA AGGTGTCCCA GACCGTGAGT 
    CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAA TTGGAGAAGT TTTGCACAAG
    TTTGCACTTC AGGATGATTT TGCAGACTTT TCAGAAAGCA GGATTTCCAG TGCCAGACTT
    AACAATTTTT CTCGCTTGGA GCCCACACTT CACCTTTTGG GTGTGCAATT TGATCAGAAT
    GTGGCCCACA ATATCATCAC AGAGAAGCCT GGGGCTGCCA CGAAACTCTT ATATCAGTTG
    TACATTGCTC TCCAGAGGAA AAAGAAAGGT GTTCAGACTA GAGTGGAAAT GCCAACTGTA
    CAGCCCCTGA TGAGCACAAA AGTTCAAAAC ATGAAAAGCG AGGCTTTCCG AGATCAACGC
    TTGAGCAAAA GACGACAAAA TGAAATCATG GCCAAAATCC AAGCAGCTAT CATACAGATT
    CCTAAACCCA CATCAAATCG TACTCTGAAA ACATTTGACG CCCAAAAATT AATGAAAAAG
    AAAAAAGAGG CAGAGGATGT GGCCAATGAA ATTAAGAAGT TTGAAGCATT AATAAAAAAG
    GATCTCCAGG CAAAAGAAAG TGCATCCAAG ACTTCTCTAG ATACAGCAGG CCAGACAACC
    GCTGATTTGT TAAACACTTA TTCAGATGAT GACTACATTA AAAAAATCCA AAAACGCCTT
    GAAGAAGATG CTTTTGCACG AGAGCAAAGG GAGAAAAGAC GGCGGAGACT CTTAATGGAT
    CAGTTAATAG CTCATGAAGC CCAAGAGGAG GCTTATCGGG AGGAGCAGTT GATCAACCGG
    CTGATGCGAC AGTCCCAGCA GGAACGCAGG ATCGCTGTGC AGCTCATGCA CGTTAGGCAT
    GAAAAGGAAG TTTTATGGCA AAACAGAATT TTCAGAGAAA AACAATATGA GGAAAGACGG
    CTTAAAGACT TCCAGGATGC TCTTGATCGA GAAGCGGCTT TAGCCAGACA AGCCAAGATT
    GATTTTGAAG AACAAGCCCT CAGAGATAAG GAAATTCATG AGCAGATCGC CATAGAAAGA
    GCTCAAGCTC GTTACAAAAA GCATTATTCA ATATGTGCAG AAATTTTGGA TCAGATACTT
    GATCTGTCCA CTAAGGTGGC AGACTATCGA TTGTTGACAA ATAACCTGAT CCCATATAAG
    TTGATGCATG ATTGGAAGGA ACTGTTTTTC CATGGAAAAC CCATATATGA GCAAGCCTCT
    CTTAGACATT TACCAGCTAA GCTCTCTGCA GAACACCTTG TAGAAATGGA GAAAAGGAAC
    TTGCTAGATA ACAATGATTA TGAAGAATAT AAGAACATGG TTGGAGAATG GGCCTTACCA
    GAAGAAATGG TTGAAAACTT ACCACCCTCC AACAATTGCA TATTGGGCCA TGTGATTCAC
    AGACTAATTG AAAGTTCTCT TCCTCCTCAA GTTGAATCTA CAGTACCTGA GTTACCTTCA
    TTTGCTATAA AAGGATGCTT GTTGGGGAAA ACCTTTAGTG GAAAAACTAC TGCTCTAAAG
    GCTCTAGAGA AAAGCTTTCC TATTCAGGTA CTTTCTATTG ACACTCTTGT TCAAGAAGCT
    ATCCAAGCAT TTCATGACAA TGAAAGTATC AGCGGGGCCC ATGGACTTCA GAAAGCACCT
    GAAGAAAAGG TTTTACCAGT TAGGCGAAAG AGTGGTAAAG AAAACCAGGA CGTGCAAGAT
    GAAGCACTAC CAGAAAGAGA AGATAAAACC AGACTTGATA CCGATGCTAG AAAAGCAGCA
    AAAGCTGAAG AAGTCGTAGC GAGGGATAGT TTCTCTGAAC TCACTGTGCG TGCTCAGCTT
    GGTGCAAAAT CAGAAATGCT GCTGAAGAAA GGAAAGAGCA TATCTGATAT ATTGCTAGTC
    AGCATCATGG TAAATGCCAT TAATCAGATA CCTATGTATA AAGGCTGGAT CCTGGATGGC
    TTTCCAATGA CATTAAACCA AGCAAAACTT CTGGAGGAAG CACTCACAGG CTACAACAGA
    AACCTCATAG AACTGGAGGG AAAGAAATCA CAAACATCCA CATTAGCTGT TGACCCTACA
    GCTTCCAAAG AAGTGCCTCT TCCCCCTTCT GCATTTGATT TTGTCATGTT ATTAGACATT
    TCAGATAATT CCTCACTGAA TCGCATGAAT GACATCATGG CTAAAGCATT TTCAAGTGAA
    GCTCCTCGTG AAGATATCAA TCAGCGTGTA GCTGCTAAAA ACAAAGATAC AGATGAGGAC
    CAGAATTTAA GAGATCAAAT ACAGCACAGA ATTGTAGGCT TCCTGGACAA CTGGCCTTCG
    TTGGAGCAGT GGTTTACAGA GCCAGAAAAC ATTTTGATAA AAATCAATGC TGAAGTAGAT
    AAACAGTCAT TGTTCCAAAA AGTAAAGGAA ATGATTATGA CTGAAATAAT TAAAAAAGAA
    AATAAAGTGA AAAAGAAATT AGAAGAAAAG GAAGCTGAGA GAAAAGAAGA AGTTTCCCTG
    AGTGAGAAGA CATCCCCTCC TCCTCCTCCC CCTCCGGAAC CAGAGAAAGA GAAGGAAGTC
    CACCTTCCAC AGGAGCGGTC CAAAACTCCT GCTGCAAAAG GAAAGCAAGC ATCAGAACCC
    CTGCATGGTA ATCGAGAACC TCTTCAGGAA GGAAAAGGGA AGAAAAGTGA AACTTCACTC
    AAAAGAAAAG GTTCTCCTAG AGGAAAATCG CTAGGAGGGA AGATTCCACT CAAGAAATCA
    CCTGCCGAGT CTACAGATAT TTCACCGGTC CCGGTAGTGC CAGCCCCATC GAAGCCCGGA
    TCAGAAGAGT GGGTGTATGT AAATGAACCA ATTCCTGAGG AAATACCATC ATTTTTAGTA
    CCATACTGGA AATTGATAGA AAACTCCTAT ATAAATTCCA TCAAAACAGT ACTCAGGGAG
    TTGAGAGAAG GCCAGCATAG TGTACTTGCT TACTTATATG ATATTAGAAC AGGCTTCCAG
    CAGTTTCTGA AGCGTCCAGA CCACAAGCAA GACTTCGTGT CTCAGTGGCA GGCGGATTTC
    AACTCCCTTC CTGATGACCT GTGGGATGAT GAGGAAACAA AGGCTGAGCT GCACCAAAGA
    GTGAATGATT TACGGGACCG CCTGTGGGAC ATTTGCGATG CCCGTAAGGA AGAAGCAGAG
    CAGGAGCGTC TGGACATCAT TAACGAGAGC TGGTTGCAGG ACTCAATGGG AATTGCCATG
    AACCATTTCT TCTCACTGAT GCAGGCAGAA CTGAACCGTT TCCAAGATAC GAAGAGATTC
    CTGCAAGATT ATTACAGGGC CATGGAATGT AAAATTCCGA TGGATGACAC TAAGAAATTC
    ACTCGAGTCC CACTGGTCCG ACTGGATAGT AGAGATATTT TGGAAAGCCA GCTTAGAATT
    CCCCTGGTTC CTCGAAGATC CATTTCTCCA GAGTCAGCTT TATCCAAACC AAAAACAAAA
    AGCATCCTGA AGGGCAAGAT CGATTACACG CTGGAAAGTG TAGAGTTAAA CTTTGAGACT
    GATGAGAAGA TGGTGATGGA TACGTGGCAG CAGTCTTCTT TGGCAGTATC TCACATGGTG
    GCTGCTGAAA TTCATCAGAG GCTCATGGAA GAAGAAAAAG AAAACCAGCC AGCAGAAACA
    AAAGAAAAAC TTCCTCAGGC AGCTGCAAAT AAAAAAGTGA AAAAGGAGCC TCCGCCACCG
    CCACCACCAC CCAAGAAAAA GAAAGAGGAC AAAAAAGGAA AAGGTAAATC CCCACCTATG
    GCAGAAGCTA CTCCTGTAGT AGTGCCAGCA GAAGAGACTG AGGAAGTGGA ATTAAAAAAT
    GAACTGAAGC TCAGAATAAA GGAAGAGCAT CTTGCTGCCC TGCAATTTGA AGAGATAGCT
    ACACAGTTCC GACTTGAACT GATAAAGGTA AAAGCATTGG CTTTCCTGGA AGAGTTAGTA
    ATAAAGGCGG TTGATGTATA TAGACTCATG GACAAGTGGC TCGGTGAGAG GTACTTGAAT
    GAAATGGCCA GTGTGGAAAA ACTAACTGGG GTAGCTCGCT ATCACATTGA AACATCAACC
    AAAATTCAGA ATGAACTTTA TTTAGACCAA GAAGACTTCT TCATTAATGG CGATATCAAA
    GTCTTTCCAG ATCCACCTCC TCCGGTACGC CCTCCGCCTG TGGAAAAGGA GGAAAATGGC
    ACCCTGACCA TGGAGCAGCT TGACAACCTC CGGGATCAGT TCTTAGATAT AGCGCCCAAA
    GGCAGGGATC CTCCAGACAA TGTGAGTGGA TTCACCAAGA AGCAGAAAAG GCCTCAGAAG
    CTTGACCATT ACCTCCCAAA GAGGAACAAT CAATTTTTGA AAATTTGGCT TAAAAAATAC
    CCTTGGCTTG TATACGATGA GATACTAAAT CTTATGTTCT GCGGCCTGTG TCGTAAACAT
    GGAGTGACAT CAAAGGGAAA TCAGGTCAGT TTTTTTTACG GCACGGATAA CTTCAGGACT
    GAATTTCTCA ACGCGCATCA TCTGAGTGAG GCTCACGCCA AGGCGTCCCT CATGGAAGCC
    ACAAGTGGTT CTCCAGGGAG CAGAGCTACC ACGGAGCTGA TGGTGAGGAC CATGAGCAAG
    GTGACCCTTG GCAGGGTGGA GAACCTGTTC CGGTCCTGCC ACGCCATCGC CAAGACCGGA
    CGTCCCCTCA AAGACTTCCT CTGGATGTGT TCGCTGGACG ATATGAAGGG AATTGATATC
    GGCCCGGTGT TCAGAACCAG TAAATCGGCC AGAACTTTTA CCTACTTTAT TGCTGAAGTG
    GAACGGAGAA CTCTAAGAGA GAAGTTAGAA AAAAGTCAGT TCTTCTCCGT CCTCAGTGAT
    GGGATTGCAG ACAATTCAGC TGAGGAAGCT GAACTCGTAT ATGTGCAGTT TGCCCGCGCA
    GGGAGAGTGC ACTGCCAAAT CGTCGGGGTA CAGACCGTGG AGAAGAAGGA GCCCTGGGCG
    ATAAAACGTG CCATTGAGAA AACTCTGGAG AGAAATCTTC AACTTCGACT GTCGAACCAA
    GACTGGGCAA AGAAACTGGT GGGTTTCGGA AGCGATGGAG CTCCAGGTTC AGAGGGAGAA
    AACGGCAGCA CAGTGGCCCT GCTTTTAAGG GAAATCCAGC CAGGTGTGCT GCCTGTTTAC
    TGCCTTGCCC ATCACCTCGA ACTGTCTTAC CAGGCGGTGT TCCAGAGCGT TCCCCTCTAC
    AGCCGTGTGA AAGACCTTCT TCATAGCATG CACCGCTTCT ATCACGATTC TCCTGCTCAC
    AGGAGTGCTC TGGTGACTGC TTTCAAGGGC CTCCACCTTC AACCTGCGAT GCCCTCTCAA
    GTCAGAGGCC GGAGGTGGCT TCAGGGATTG CAGGCTGCCC TCCAGAACTT TCTCAAGGGA
    TATCCAGCAA TTGTCCAGCA GCTGCAGTCA ACAAAAGAAA GCAGAAGCAA CAGCAGTCAT
    CAGAAAGCCG AAGAACTCCT GGAGTCTCTC CTCCAGGCAG ACATTGTCAA ATTTGTCCAC
    TTCTTGGTGG ATGTCGTCAA TGTCCTTAGC ATACTGTCCC ATGTCAATCA GAACAGAAAT
    TCCTCAATTG CTGATGTATT TGCCACCTTA GAGTCAACAC TGGAAATGCT CCAAATTTAT
    CAGACAAGAC CAGGGCCGAA AGAACGTGGG GTAGATTCGG TCACACACTT TCATGGTAAC
    TGCCTTGGAG GAAAGGGAAA CATTTCTGAC GTGAGAAACA TTGTTTTAAC ACATCTTATC
    AAGAGGCTGC GAGGCTGTTT CAGAGATGCC AACCAAGATG TGGTGAAGGC AACCACGATT
    GGAAGCTTTA AACTGTGGCC GGCAAAAATA AATCAAGAAT TTGGAGAAAA AGAGGTGGCC
    ATCCTGATTG CACATTATGA ACCAGTATTA GAGGCAGCCA AGGTGAAAAT TGACCAAGTA
    GACACAGAAT GGAGCATGTT GAAATTGGAG ATCTACTCTA GATTTCAGAA CATTTGCAAA
    CTGACCTGGG ATTTTGTGAA TTCTGTTTAT TTACACAAGT ATCCAAATAT CCTGACACTC
    ATTGACCTCG TGCTGAGCCT GCCTGCAAGT TCAGCTGAGG CAGGACGTGG CTTTAGTCAG
    ATGAAGCGCA TCAGGTCCCA AATGCATGCC AAGGTCAAGG CTGACAACAT GACGGATCTC
    CTGATAATTC AGTTGAATTC TCCAGACATT AACAACTTTG ACCCTAGGAA AGCCATTCAT
    TTATGGAATA CAAGAACACC ATCCTCAACT GAGGACACAA GACCTAACTC AGACTGCTCA
    TCAAACTCAG AAAGCCTTAG TGAAAGCGAT GAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OSm32424
    Clone ID Related Accession (Same CDS sequence) XM_012097076.2
    Accession Version XM_012097076.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6285bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-16
    Organism Ovis aries(sheep)
    Product sperm flagellar protein 2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014639025.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012097076.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGCAAGGTC CCAAGTCATT TGCAAAGGCA TTTTCCAGTG GCTATCTAAT TGGAGAAGTT 
    TTGCACAAGT TTGCACTTCA GGATGATTTT GCAGACTTTT CAGAAAGCAG GATTTCCAGT
    GCCAGACTTA ACAATTTTTC TCGCTTGGAG CCCACACTTC ACCTTTTGGG TGTGCAATTT
    GATCAGAATG TGGCCCACAA TATCATCACA GAGAAGCCTG GGGCTGCCAC GAAACTCTTA
    TATCAGTTGT ACATTGCTCT CCAGAGGAAA AAGAAAGGTG TTCAGACTAG AGTGGAAATG
    CCAACTGTAC AGCCCCTGAT GAGCACAAAA GTTCAAAACA TGAAAAGCGA GGCTTTCCGA
    GATCAACGCT TGAGCAAAAG ACGACAAAAT GAAATCATGG CCAAAATCCA AGCAGCTATC
    ATACAGATTC CTAAACCCAC ATCAAATCGT ACTCTGAAAA CATTTGACGC CCAAAAATTA
    ATGAAAAAGA AAAAAGAGGC AGAGGATGTG GCCAATGAAA TTAAGAAGTT TGAAGCATTA
    ATAAAAAAGG ATCTCCAGGC AAAAGAAAGT GCATCCAAGA CTTCTCTAGA TACAGCAGGC
    CAGACAACCG CTGATTTGTT AAACACTTAT TCAGATGATG ACTACATTAA AAAAATCCAA
    AAACGCCTTG AAGAAGATGC TTTTGCACGA GAGCAAAGGG AGAAAAGACG GCGGAGACTC
    TTAATGGATC AGTTAATAGC TCATGAAGCC CAAGAGGAGG CTTATCGGGA GGAGCAGTTG
    ATCAACCGGC TGATGCGACA GTCCCAGCAG GAACGCAGGA TCGCTGTGCA GCTCATGCAC
    GTTAGGCATG AAAAGGAAGT TTTATGGCAA AACAGAATTT TCAGAGAAAA ACAATATGAG
    GAAAGACGGC TTAAAGACTT CCAGGATGCT CTTGATCGAG AAGCGGCTTT AGCCAGACAA
    GCCAAGATTG ATTTTGAAGA ACAAGCCCTC AGAGATAAGG AAATTCATGA GCAGATCGCC
    ATAGAAAGAG CTCAAGCTCG TTACAAAAAG CATTATTCAA TATGTGCAGA AATTTTGGAT
    CAGATACTTG ATCTGTCCAC TAAGGTGGCA GACTATCGAT TGTTGACAAA TAACCTGATC
    CCATATAAGT TGATGCATGA TTGGAAGGAA CTGTTTTTCC ATGGAAAACC CATATATGAG
    CAAGCCTCTC TTAGACATTT ACCAGCTAAG CTCTCTGCAG AACACCTTGT AGAAATGGAG
    AAAAGGAACT TGCTAGATAA CAATGATTAT GAAGAATATA AGAACATGGT TGGAGAATGG
    GCCTTACCAG AAGAAATGGT TGAAAACTTA CCACCCTCCA ACAATTGCAT ATTGGGCCAT
    GTGATTCACA GACTAATTGA AAGTTCTCTT CCTCCTCAAG TTGAATCTAC AGTACCTGAG
    TTACCTTCAT TTGCTATAAA AGGATGCTTG TTGGGGAAAA CCTTTAGTGG AAAAACTACT
    GCTCTAAAGG CTCTAGAGAA AAGCTTTCCT ATTCAGGTAC TTTCTATTGA CACTCTTGTT
    CAAGAAGCTA TCCAAGCATT TCATGACAAT GAAAGTATCA GCGGGGCCCA TGGACTTCAG
    AAAGCACCTG AAGAAAAGGT TTTACCAGTT AGGCGAAAGA GTGGTAAAGA AAACCAGGAC
    GTGCAAGATG AAGCACTACC AGAAAGAGAA GATAAAACCA GACTTGATAC CGATGCTAGA
    AAAGCAGCAA AAGCTGAAGA AGTCGTAGCG AGGGATAGTT TCTCTGAACT CACTGTGCGT
    GCTCAGCTTG GTGCAAAATC AGAAATGCTG CTGAAGAAAG GAAAGAGCAT ATCTGATATA
    TTGCTAGTCA GCATCATGGT AAATGCCATT AATCAGATAC CTATGTATAA AGGCTGGATC
    CTGGATGGCT TTCCAATGAC ATTAAACCAA GCAAAACTTC TGGAGGAAGC ACTCACAGGC
    TACAACAGAA ACCTCATAGA ACTGGAGGGA AAGAAATCAC AAACATCCAC ATTAGCTGTT
    GACCCTACAG CTTCCAAAGA AGTGCCTCTT CCCCCTTCTG CATTTGATTT TGTCATGTTA
    TTAGACATTT CAGATAATTC CTCACTGAAT CGCATGAATG ACATCATGGC TAAAGCATTT
    TCAAGTGAAG CTCCTCGTGA AGATATCAAT CAGCGTGTAG CTGCTAAAAA CAAAGATACA
    GATGAGGACC AGAATTTAAG AGATCAAATA CAGCACAGAA TTGTAGGCTT CCTGGACAAC
    TGGCCTTCGT TGGAGCAGTG GTTTACAGAG CCAGAAAACA TTTTGATAAA AATCAATGCT
    GAAGTAGATA AACAGTCATT GTTCCAAAAA GTAAAGGAAA TGATTATGAC TGAAATAATT
    AAAAAAGAAA ATAAAGTGAA AAAGAAATTA GAAGAAAAGG AAGCTGAGAG AAAAGAAGAA
    GTTTCCCTGA GTGAGAAGAC ATCCCCTCCT CCTCCTCCCC CTCCGGAACC AGAGAAAGAG
    AAGGAAGTCC ACCTTCCACA GGAGCGGTCC AAAACTCCTG CTGCAAAAGG AAAGCAAGCA
    TCAGAACCCC TGCATGGTAA TCGAGAACCT CTTCAGGAAG GAAAAGGGAA GAAAAGTGAA
    ACTTCACTCA AAAGAAAAGG TTCTCCTAGA GGAAAATCGC TAGGAGGGAA GATTCCACTC
    AAGAAATCAC CTGCCGAGTC TACAGATATT TCACCGGTCC CGGTAGTGCC AGCCCCATCG
    AAGCCCGGAT CAGAAGAGTG GGTGTATGTA AATGAACCAA TTCCTGAGGA AATACCATCA
    TTTTTAGTAC CATACTGGAA ATTGATAGAA AACTCCTATA TAAATTCCAT CAAAACAGTA
    CTCAGGGAGT TGAGAGAAGG CCAGCATAGT GTACTTGCTT ACTTATATGA TATTAGAACA
    GGCTTCCAGC AGTTTCTGAA GCGTCCAGAC CACAAGCAAG ACTTCGTGTC TCAGTGGCAG
    GCGGATTTCA ACTCCCTTCC TGATGACCTG TGGGATGATG AGGAAACAAA GGCTGAGCTG
    CACCAAAGAG TGAATGATTT ACGGGACCGC CTGTGGGACA TTTGCGATGC CCGTAAGGAA
    GAAGCAGAGC AGGAGCGTCT GGACATCATT AACGAGAGCT GGTTGCAGGA CTCAATGGGA
    ATTGCCATGA ACCATTTCTT CTCACTGATG CAGGCAGAAC TGAACCGTTT CCAAGATACG
    AAGAGATTCC TGCAAGATTA TTACAGGGCC ATGGAATGTA AAATTCCGAT GGATGACACT
    AAGAAATTCA CTCGAGTCCC ACTGGTCCGA CTGGATAGTA GAGATATTTT GGAAAGCCAG
    CTTAGAATTC CCCTGGTTCC TCGAAGATCC ATTTCTCCAG AGTCAGCTTT ATCCAAACCA
    AAAACAAAAA GCATCCTGAA GGGCAAGATC GATTACACGC TGGAAAGTGT AGAGTTAAAC
    TTTGAGACTG ATGAGAAGAT GGTGATGGAT ACGTGGCAGC AGTCTTCTTT GGCAGTATCT
    CACATGGTGG CTGCTGAAAT TCATCAGAGG CTCATGGAAG AAGAAAAAGA AAACCAGCCA
    GCAGAAACAA AAGAAAAACT TCCTCAGGCA GCTGCAAATA AAAAAGTGAA AAAGGAGCCT
    CCGCCACCGC CACCACCACC CAAGAAAAAG AAAGAGGACA AAAAAGGAAA AGGTAAATCC
    CCACCTATGG CAGAAGCTAC TCCTGTAGTA GTGCCAGCAG AAGAGACTGA GGAAGTGGAA
    TTAAAAAATG AACTGAAGCT CAGAATAAAG GAAGAGCATC TTGCTGCCCT GCAATTTGAA
    GAGATAGCTA CACAGTTCCG ACTTGAACTG ATAAAGGTAA AAGCATTGGC TTTCCTGGAA
    GAGTTAGTAA TAAAGGCGGT TGATGTATAT AGACTCATGG ACAAGTGGCT CGGTGAGAGG
    TACTTGAATG AAATGGCCAG TGTGGAAAAA CTAACTGGGG TAGCTCGCTA TCACATTGAA
    ACATCAACCA AAATTCAGAA TGAACTTTAT TTAGACCAAG AAGACTTCTT CATTAATGGC
    GATATCAAAG TCTTTCCAGA TCCACCTCCT CCGGTACGCC CTCCGCCTGT GGAAAAGGAG
    GAAAATGGCA CCCTGACCAT GGAGCAGCTT GACAACCTCC GGGATCAGTT CTTAGATATA
    GCGCCCAAAG GCAGGGATCC TCCAGACAAT GTGAGTGGAT TCACCAAGAA GCAGAAAAGG
    CCTCAGAAGC TTGACCATTA CCTCCCAAAG AGGAACAATC AATTTTTGAA AATTTGGCTT
    AAAAAATACC CTTGGCTTGT ATACGATGAG ATACTAAATC TTATGTTCTG CGGCCTGTGT
    CGTAAACATG GAGTGACATC AAAGGGAAAT CAGGTCAGTT TTTTTTACGG CACGGATAAC
    TTCAGGACTG AATTTCTCAA CGCGCATCAT CTGAGTGAGG CTCACGCCAA GGCGTCCCTC
    ATGGAAGCCA CAAGTGGTTC TCCAGGGAGC AGAGCTACCA CGGAGCTGAT GGTGAGGACC
    ATGAGCAAGG TGACCCTTGG CAGGGTGGAG AACCTGTTCC GGTCCTGCCA CGCCATCGCC
    AAGACCGGAC GTCCCCTCAA AGACTTCCTC TGGATGTGTT CGCTGGACGA TATGAAGGGA
    ATTGATATCG GCCCGGTGTT CAGAACCAGT AAATCGGCCA GAACTTTTAC CTACTTTATT
    GCTGAAGTGG AACGGAGAAC TCTAAGAGAG AAGTTAGAAA AAAGTCAGTT CTTCTCCGTC
    CTCAGTGATG GGATTGCAGA CAATTCAGCT GAGGAAGCTG AACTCGTATA TGTGCAGTTT
    GCCCGCGCAG GGAGAGTGCA CTGCCAAATC GTCGGGGTAC AGACCGTGGA GAAGAAGGAG
    CCCTGGGCGA TAAAACGTGC CATTGAGAAA ACTCTGGAGA GAAATCTTCA ACTTCGACTG
    TCGAACCAAG ACTGGGCAAA GAAACTGGTG GGTTTCGGAA GCGATGGAGC TCCAGGTTCA
    GAGGGAGAAA ACGGCAGCAC AGTGGCCCTG CTTTTAAGGG AAATCCAGCC AGGTGTGCTG
    CCTGTTTACT GCCTTGCCCA TCACCTCGAA CTGTCTTACC AGGCGGTGTT CCAGAGCGTT
    CCCCTCTACA GCCGTGTGAA AGACCTTCTT CATAGCATGC ACCGCTTCTA TCACGATTCT
    CCTGCTCACA GGAGTGCTCT GGTGACTGCT TTCAAGGGCC TCCACCTTCA ACCTGCGATG
    CCCTCTCAAG TCAGAGGCCG GAGGTGGCTT CAGGGATTGC AGGCTGCCCT CCAGAACTTT
    CTCAAGGGAT ATCCAGCAAT TGTCCAGCAG CTGCAGTCAA CAAAAGAAAG CAGAAGCAAC
    AGCAGTCATC AGAAAGCCGA AGAACTCCTG GAGTCTCTCC TCCAGGCAGA CATTGTCAAA
    TTTGTCCACT TCTTGGTGGA TGTCGTCAAT GTCCTTAGCA TACTGTCCCA TGTCAATCAG
    AACAGAAATT CCTCAATTGC TGATGTATTT GCCACCTTAG AGTCAACACT GGAAATGCTC
    CAAATTTATC AGACAAGACC AGGGCCGAAA GAACGTGGGG TAGATTCGGT CACACACTTT
    CATGGTAACT GCCTTGGAGG AAAGGGAAAC ATTTCTGACG TGAGAAACAT TGTTTTAACA
    CATCTTATCA AGAGGCTGCG AGGCTGTTTC AGAGATGCCA ACCAAGATGT GGTGAAGGCA
    ACCACGATTG GAAGCTTTAA ACTGTGGCCG GCAAAAATAA ATCAAGAATT TGGAGAAAAA
    GAGGTGGCCA TCCTGATTGC ACATTATGAA CCAGTATTAG AGGCAGCCAA GGTGAAAATT
    GACCAAGTAG ACACAGAATG GAGCATGTTG AAATTGGAGA TCTACTCTAG ATTTCAGAAC
    ATTTGCAAAC TGACCTGGGA TTTTGTGAAT TCTGTTTATT TACACAAGTA TCCAAATATC
    CTGACACTCA TTGACCTCGT GCTGAGCCTG CCTGCAAGTT CAGCTGAGGC AGGACGTGGC
    TTTAGTCAGA TGAAGCGCAT CAGGTCCCAA ATGCATGCCA AGGTCAAGGC TGACAACATG
    ACGGATCTCC TGATAATTCA GTTGAATTCT CCAGACATTA ACAACTTTGA CCCTAGGAAA
    GCCATTCATT TATGGAATAC AAGAACACCA TCCTCAACTG AGGACACAAG ACCTAACTCA
    GACTGCTCAT CAAACTCAGA AAGCCTTAGT GAAAGCGATG AGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011952466.1
    CDS2216..8500
    Translation

    Target ORF information:

    RefSeq Version XM_012097076.2
    Organism Ovis aries(sheep)
    Definition Ovis aries sperm flagellar 2 (SPEF2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012097076.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGCAAGGTC CCAAGTCATT TGCAAAGGCA TTTTCCAGTG GCTATCTAAT TGGAGAAGTT 
    TTGCACAAGT TTGCACTTCA GGATGATTTT GCAGACTTTT CAGAAAGCAG GATTTCCAGT
    GCCAGACTTA ACAATTTTTC TCGCTTGGAG CCCACACTTC ACCTTTTGGG TGTGCAATTT
    GATCAGAATG TGGCCCACAA TATCATCACA GAGAAGCCTG GGGCTGCCAC GAAACTCTTA
    TATCAGTTGT ACATTGCTCT CCAGAGGAAA AAGAAAGGTG TTCAGACTAG AGTGGAAATG
    CCAACTGTAC AGCCCCTGAT GAGCACAAAA GTTCAAAACA TGAAAAGCGA GGCTTTCCGA
    GATCAACGCT TGAGCAAAAG ACGACAAAAT GAAATCATGG CCAAAATCCA AGCAGCTATC
    ATACAGATTC CTAAACCCAC ATCAAATCGT ACTCTGAAAA CATTTGACGC CCAAAAATTA
    ATGAAAAAGA AAAAAGAGGC AGAGGATGTG GCCAATGAAA TTAAGAAGTT TGAAGCATTA
    ATAAAAAAGG ATCTCCAGGC AAAAGAAAGT GCATCCAAGA CTTCTCTAGA TACAGCAGGC
    CAGACAACCG CTGATTTGTT AAACACTTAT TCAGATGATG ACTACATTAA AAAAATCCAA
    AAACGCCTTG AAGAAGATGC TTTTGCACGA GAGCAAAGGG AGAAAAGACG GCGGAGACTC
    TTAATGGATC AGTTAATAGC TCATGAAGCC CAAGAGGAGG CTTATCGGGA GGAGCAGTTG
    ATCAACCGGC TGATGCGACA GTCCCAGCAG GAACGCAGGA TCGCTGTGCA GCTCATGCAC
    GTTAGGCATG AAAAGGAAGT TTTATGGCAA AACAGAATTT TCAGAGAAAA ACAATATGAG
    GAAAGACGGC TTAAAGACTT CCAGGATGCT CTTGATCGAG AAGCGGCTTT AGCCAGACAA
    GCCAAGATTG ATTTTGAAGA ACAAGCCCTC AGAGATAAGG AAATTCATGA GCAGATCGCC
    ATAGAAAGAG CTCAAGCTCG TTACAAAAAG CATTATTCAA TATGTGCAGA AATTTTGGAT
    CAGATACTTG ATCTGTCCAC TAAGGTGGCA GACTATCGAT TGTTGACAAA TAACCTGATC
    CCATATAAGT TGATGCATGA TTGGAAGGAA CTGTTTTTCC ATGGAAAACC CATATATGAG
    CAAGCCTCTC TTAGACATTT ACCAGCTAAG CTCTCTGCAG AACACCTTGT AGAAATGGAG
    AAAAGGAACT TGCTAGATAA CAATGATTAT GAAGAATATA AGAACATGGT TGGAGAATGG
    GCCTTACCAG AAGAAATGGT TGAAAACTTA CCACCCTCCA ACAATTGCAT ATTGGGCCAT
    GTGATTCACA GACTAATTGA AAGTTCTCTT CCTCCTCAAG TTGAATCTAC AGTACCTGAG
    TTACCTTCAT TTGCTATAAA AGGATGCTTG TTGGGGAAAA CCTTTAGTGG AAAAACTACT
    GCTCTAAAGG CTCTAGAGAA AAGCTTTCCT ATTCAGGTAC TTTCTATTGA CACTCTTGTT
    CAAGAAGCTA TCCAAGCATT TCATGACAAT GAAAGTATCA GCGGGGCCCA TGGACTTCAG
    AAAGCACCTG AAGAAAAGGT TTTACCAGTT AGGCGAAAGA GTGGTAAAGA AAACCAGGAC
    GTGCAAGATG AAGCACTACC AGAAAGAGAA GATAAAACCA GACTTGATAC CGATGCTAGA
    AAAGCAGCAA AAGCTGAAGA AGTCGTAGCG AGGGATAGTT TCTCTGAACT CACTGTGCGT
    GCTCAGCTTG GTGCAAAATC AGAAATGCTG CTGAAGAAAG GAAAGAGCAT ATCTGATATA
    TTGCTAGTCA GCATCATGGT AAATGCCATT AATCAGATAC CTATGTATAA AGGCTGGATC
    CTGGATGGCT TTCCAATGAC ATTAAACCAA GCAAAACTTC TGGAGGAAGC ACTCACAGGC
    TACAACAGAA ACCTCATAGA ACTGGAGGGA AAGAAATCAC AAACATCCAC ATTAGCTGTT
    GACCCTACAG CTTCCAAAGA AGTGCCTCTT CCCCCTTCTG CATTTGATTT TGTCATGTTA
    TTAGACATTT CAGATAATTC CTCACTGAAT CGCATGAATG ACATCATGGC TAAAGCATTT
    TCAAGTGAAG CTCCTCGTGA AGATATCAAT CAGCGTGTAG CTGCTAAAAA CAAAGATACA
    GATGAGGACC AGAATTTAAG AGATCAAATA CAGCACAGAA TTGTAGGCTT CCTGGACAAC
    TGGCCTTCGT TGGAGCAGTG GTTTACAGAG CCAGAAAACA TTTTGATAAA AATCAATGCT
    GAAGTAGATA AACAGTCATT GTTCCAAAAA GTAAAGGAAA TGATTATGAC TGAAATAATT
    AAAAAAGAAA ATAAAGTGAA AAAGAAATTA GAAGAAAAGG AAGCTGAGAG AAAAGAAGAA
    GTTTCCCTGA GTGAGAAGAC ATCCCCTCCT CCTCCTCCCC CTCCGGAACC AGAGAAAGAG
    AAGGAAGTCC ACCTTCCACA GGAGCGGTCC AAAACTCCTG CTGCAAAAGG AAAGCAAGCA
    TCAGAACCCC TGCATGGTAA TCGAGAACCT CTTCAGGAAG GAAAAGGGAA GAAAAGTGAA
    ACTTCACTCA AAAGAAAAGG TTCTCCTAGA GGAAAATCGC TAGGAGGGAA GATTCCACTC
    AAGAAATCAC CTGCCGAGTC TACAGATATT TCACCGGTCC CGGTAGTGCC AGCCCCATCG
    AAGCCCGGAT CAGAAGAGTG GGTGTATGTA AATGAACCAA TTCCTGAGGA AATACCATCA
    TTTTTAGTAC CATACTGGAA ATTGATAGAA AACTCCTATA TAAATTCCAT CAAAACAGTA
    CTCAGGGAGT TGAGAGAAGG CCAGCATAGT GTACTTGCTT ACTTATATGA TATTAGAACA
    GGCTTCCAGC AGTTTCTGAA GCGTCCAGAC CACAAGCAAG ACTTCGTGTC TCAGTGGCAG
    GCGGATTTCA ACTCCCTTCC TGATGACCTG TGGGATGATG AGGAAACAAA GGCTGAGCTG
    CACCAAAGAG TGAATGATTT ACGGGACCGC CTGTGGGACA TTTGCGATGC CCGTAAGGAA
    GAAGCAGAGC AGGAGCGTCT GGACATCATT AACGAGAGCT GGTTGCAGGA CTCAATGGGA
    ATTGCCATGA ACCATTTCTT CTCACTGATG CAGGCAGAAC TGAACCGTTT CCAAGATACG
    AAGAGATTCC TGCAAGATTA TTACAGGGCC ATGGAATGTA AAATTCCGAT GGATGACACT
    AAGAAATTCA CTCGAGTCCC ACTGGTCCGA CTGGATAGTA GAGATATTTT GGAAAGCCAG
    CTTAGAATTC CCCTGGTTCC TCGAAGATCC ATTTCTCCAG AGTCAGCTTT ATCCAAACCA
    AAAACAAAAA GCATCCTGAA GGGCAAGATC GATTACACGC TGGAAAGTGT AGAGTTAAAC
    TTTGAGACTG ATGAGAAGAT GGTGATGGAT ACGTGGCAGC AGTCTTCTTT GGCAGTATCT
    CACATGGTGG CTGCTGAAAT TCATCAGAGG CTCATGGAAG AAGAAAAAGA AAACCAGCCA
    GCAGAAACAA AAGAAAAACT TCCTCAGGCA GCTGCAAATA AAAAAGTGAA AAAGGAGCCT
    CCGCCACCGC CACCACCACC CAAGAAAAAG AAAGAGGACA AAAAAGGAAA AGGTAAATCC
    CCACCTATGG CAGAAGCTAC TCCTGTAGTA GTGCCAGCAG AAGAGACTGA GGAAGTGGAA
    TTAAAAAATG AACTGAAGCT CAGAATAAAG GAAGAGCATC TTGCTGCCCT GCAATTTGAA
    GAGATAGCTA CACAGTTCCG ACTTGAACTG ATAAAGGTAA AAGCATTGGC TTTCCTGGAA
    GAGTTAGTAA TAAAGGCGGT TGATGTATAT AGACTCATGG ACAAGTGGCT CGGTGAGAGG
    TACTTGAATG AAATGGCCAG TGTGGAAAAA CTAACTGGGG TAGCTCGCTA TCACATTGAA
    ACATCAACCA AAATTCAGAA TGAACTTTAT TTAGACCAAG AAGACTTCTT CATTAATGGC
    GATATCAAAG TCTTTCCAGA TCCACCTCCT CCGGTACGCC CTCCGCCTGT GGAAAAGGAG
    GAAAATGGCA CCCTGACCAT GGAGCAGCTT GACAACCTCC GGGATCAGTT CTTAGATATA
    GCGCCCAAAG GCAGGGATCC TCCAGACAAT GTGAGTGGAT TCACCAAGAA GCAGAAAAGG
    CCTCAGAAGC TTGACCATTA CCTCCCAAAG AGGAACAATC AATTTTTGAA AATTTGGCTT
    AAAAAATACC CTTGGCTTGT ATACGATGAG ATACTAAATC TTATGTTCTG CGGCCTGTGT
    CGTAAACATG GAGTGACATC AAAGGGAAAT CAGGTCAGTT TTTTTTACGG CACGGATAAC
    TTCAGGACTG AATTTCTCAA CGCGCATCAT CTGAGTGAGG CTCACGCCAA GGCGTCCCTC
    ATGGAAGCCA CAAGTGGTTC TCCAGGGAGC AGAGCTACCA CGGAGCTGAT GGTGAGGACC
    ATGAGCAAGG TGACCCTTGG CAGGGTGGAG AACCTGTTCC GGTCCTGCCA CGCCATCGCC
    AAGACCGGAC GTCCCCTCAA AGACTTCCTC TGGATGTGTT CGCTGGACGA TATGAAGGGA
    ATTGATATCG GCCCGGTGTT CAGAACCAGT AAATCGGCCA GAACTTTTAC CTACTTTATT
    GCTGAAGTGG AACGGAGAAC TCTAAGAGAG AAGTTAGAAA AAAGTCAGTT CTTCTCCGTC
    CTCAGTGATG GGATTGCAGA CAATTCAGCT GAGGAAGCTG AACTCGTATA TGTGCAGTTT
    GCCCGCGCAG GGAGAGTGCA CTGCCAAATC GTCGGGGTAC AGACCGTGGA GAAGAAGGAG
    CCCTGGGCGA TAAAACGTGC CATTGAGAAA ACTCTGGAGA GAAATCTTCA ACTTCGACTG
    TCGAACCAAG ACTGGGCAAA GAAACTGGTG GGTTTCGGAA GCGATGGAGC TCCAGGTTCA
    GAGGGAGAAA ACGGCAGCAC AGTGGCCCTG CTTTTAAGGG AAATCCAGCC AGGTGTGCTG
    CCTGTTTACT GCCTTGCCCA TCACCTCGAA CTGTCTTACC AGGCGGTGTT CCAGAGCGTT
    CCCCTCTACA GCCGTGTGAA AGACCTTCTT CATAGCATGC ACCGCTTCTA TCACGATTCT
    CCTGCTCACA GGAGTGCTCT GGTGACTGCT TTCAAGGGCC TCCACCTTCA ACCTGCGATG
    CCCTCTCAAG TCAGAGGCCG GAGGTGGCTT CAGGGATTGC AGGCTGCCCT CCAGAACTTT
    CTCAAGGGAT ATCCAGCAAT TGTCCAGCAG CTGCAGTCAA CAAAAGAAAG CAGAAGCAAC
    AGCAGTCATC AGAAAGCCGA AGAACTCCTG GAGTCTCTCC TCCAGGCAGA CATTGTCAAA
    TTTGTCCACT TCTTGGTGGA TGTCGTCAAT GTCCTTAGCA TACTGTCCCA TGTCAATCAG
    AACAGAAATT CCTCAATTGC TGATGTATTT GCCACCTTAG AGTCAACACT GGAAATGCTC
    CAAATTTATC AGACAAGACC AGGGCCGAAA GAACGTGGGG TAGATTCGGT CACACACTTT
    CATGGTAACT GCCTTGGAGG AAAGGGAAAC ATTTCTGACG TGAGAAACAT TGTTTTAACA
    CATCTTATCA AGAGGCTGCG AGGCTGTTTC AGAGATGCCA ACCAAGATGT GGTGAAGGCA
    ACCACGATTG GAAGCTTTAA ACTGTGGCCG GCAAAAATAA ATCAAGAATT TGGAGAAAAA
    GAGGTGGCCA TCCTGATTGC ACATTATGAA CCAGTATTAG AGGCAGCCAA GGTGAAAATT
    GACCAAGTAG ACACAGAATG GAGCATGTTG AAATTGGAGA TCTACTCTAG ATTTCAGAAC
    ATTTGCAAAC TGACCTGGGA TTTTGTGAAT TCTGTTTATT TACACAAGTA TCCAAATATC
    CTGACACTCA TTGACCTCGT GCTGAGCCTG CCTGCAAGTT CAGCTGAGGC AGGACGTGGC
    TTTAGTCAGA TGAAGCGCAT CAGGTCCCAA ATGCATGCCA AGGTCAAGGC TGACAACATG
    ACGGATCTCC TGATAATTCA GTTGAATTCT CCAGACATTA ACAACTTTGA CCCTAGGAAA
    GCCATTCATT TATGGAATAC AAGAACACCA TCCTCAACTG AGGACACAAG ACCTAACTCA
    GACTGCTCAT CAAACTCAGA AAGCCTTAGT GAAAGCGATG AGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OSm32426
    Clone ID Related Accession (Same CDS sequence) XM_012097079.2
    Accession Version XM_012097079.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6159bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-16
    Organism Ovis aries(sheep)
    Product sperm flagellar protein 2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014639025.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012097079.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGTCGGAAA TCCTGTGCCA GTGGCTCAAC CAGGAATTGA AGGTGTCCCA GACCGTGAGT 
    CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAA TTGGAGAAGT TTTGCACAAG
    TTTGCACTTC AGGATGATTT TGCAGACTTT TCAGAAAGCA GGATTTCCAG TGCCAGACTT
    AACAATTTTT CTCGCTTGGA GCCCACACTT CACCTTTTGG GTGTGCAATT TGATCAGAAT
    GTGGCCCACA ATATCATCAC AGAGAAGCCT GGGGCTGCCA CGAAACTCTT ATATCAGTTG
    TACATTGCTC TCCAGAGGAA AAAGAAAGGT GTTCAGACTA GAGTGGAAAT GCCAACTGTA
    CAGCCCCTGA TGAGCACAAA AGTTCAAAAC ATGAAAAGCG AGGCTTTCCG AGATCAACGC
    TTGAGCAAAA GACGACAAAA TGAAATCATG GCCAAAATCC AAGCAGCTAT CATACAGATT
    CCTAAACCCA CATCAAATCG TACTCTGAAA ACATTTGACG CCCAAAAATT AATGAAAAAG
    AAAAAAGAGG CAGAGGATGT GGCCAATGAA ATTAAGAAGT TTGAAGCATT AATAAAAAAG
    GATCTCCAGG CAAAAGAAAG TGCATCCAAG ACTTCTCTAG ATACAGCAGG CCAGACAACC
    GCTGATTTGT TAAACACTTA TTCAGATGAT GACTACATTA AAAAAATCCA AAAACGCCTT
    GAAGAAGATG CTTTTGCACG AGAGCAAAGG GAGAAAAGAC GGCGGAGACT CTTAATGGAT
    CAGTTAATAG CTCATGAAGC CCAAGAGGAG GCTTATCGGG AGGAGCAGTT GATCAACCGG
    CTGATGCGAC AGTCCCAGCA GGAACGCAGG ATCGCTGTGC AGCTCATGCA CGTTAGGCAT
    GAAAAGGAAG TTTTATGGCA AAACAGAATT TTCAGAGAAA AACAATATGA GGAAAGACGG
    CTTAAAGACT TCCAGGATGC TCTTGATCGA GAAGCGGCTT TAGCCAGACA AGCCAAGATT
    GATTTTGAAG AACAAGCCCT CAGAGATAAG GAAATTCATG AGCAGATCGC CATAGAAAGA
    GCTCAAGCTC GTTACAAAAA GCATTATTCA ATATGTGCAG AAATTTTGGA TCAGATACTT
    GATCTGTCCA CTAAGGTGGC AGACTATCGA TTGTTGACAA ATAACCTGAT CCCATATAAG
    TTGATGCATG ATTGGAAGGA ACTGTTTTTC CATGGAAAAC CCATATATGA GCAAGCCTCT
    CTTAGACATT TACCAGCTAA GCTCTCTGCA GAACACCTTG TAGAAATGGA GAAAAGGAAC
    TTGCTAGATA ACAATGATTA TGAAGAATAT AAGAACATGG TTGGAGAATG GGCCTTACCA
    GAAGAAATGG TTGAAAACTT ACCACCCTCC AACAATTGCA TATTGGGCCA TGTGATTCAC
    AGACTAATTG AAAGTTCTCT TCCTCCTCAA GTTGAATCTA CAGTACCTGA GTTACCTTCA
    TTTGCTATAA AAGGATGCTT GTTGGGGAAA ACCTTTAGTG GAAAAACTAC TGCTCTAAAG
    GCTCTAGAGA AAAGCTTTCC TATTCAGGTA CTTTCTATTG ACACTCTTGT TCAAGAAGCT
    ATCCAAGCAT TTCATGACAA TGAAAGTATC AGCGGGGCCC ATGGACTTCA GAAAGCACCT
    GAAGAAAAGG TTTTACCAGT TAGGCGAAAG AGTGGTAAAG AAAACCAGGA CGTGCAAGAT
    GAAGCACTAC CAGAAAGAGA AGATAAAACC AGACTTGATA CCGATGCTAG AAAAGCAGCA
    AAAGCTGAAG AAGTCGTAGC GAGGGATAGT TTCTCTGAAC TCACTGTGCG TGCTCAGCTT
    GGTGCAAAAT CAGAAATGCT GCTGAAGAAA GGAAAGAGCA TATCTGATAT ATTGCTAGTC
    AGCATCATGG TAAATGCCAT TAATCAGATA CCTATGTATA AAGGCTGGAT CCTGGATGGC
    TTTCCAATGA CATTAAACCA AGCAAAACTT CTGGAGGAAG CACTCACAGG CTACAACAGA
    AACCTCATAG AACTGGAGGG AAAGAAATCA CAAACATCCA CATTAGCTGT TGACCCTACA
    GCTTCCAAAG AAGTGCCTCT TCCCCCTTCT GCATTTGATT TTGTCATGTT ATTAGACATT
    TCAGATAATT CCTCACTGAA TCGCATGAAT GACATCATGG CTAAAGCATT TTCAAGTGAA
    GCTCCTCGTG AAGATATCAA TCAGCGTGTA GCTGCTAAAA ACAAAGATAC AGATGAGGAC
    CAGAATTTAA GAGATCAAAT ACAGCACAGA ATTGTAGGCT TCCTGGACAA CTGGCCTTCG
    TTGGAGCAGT GGTTTACAGA GCCAGAAAAC ATTTTGATAA AAATCAATGC TGAAGTAGAT
    AAACAGTCAT TGTTCCAAAA AGTAAAGGAA ATGATTATGA CTGAAATAAT TAAAAAAGAA
    AATAAAGTGA AAAAGAAATT AGAAGAAAAG GAAGCTGAGA GAAAAGAAGA AGTTTCCCTG
    AGTGAGAAGA CATCCCCTCC TCCTCCTCCC CCTCCGGAAC CAGAGAAAGA GAAGGAAGTC
    CACCTTCCAC AGGAGCGGTC CAAAACTCCT GCTGCAAAAG GAAAGCAAGC ATCAGAACCC
    CTGCATGGTA ATCGAGAACC TCTTCAGGAA GGAAAAGGGA AGAAAAGTGA AACTTCACTC
    AAAAGAAAAG GTTCTCCTAG AGGAAAATCG CTAGGAGGGA AGATTCCACT CAAGAAATCA
    CCTGCCGAGT CTACAGATAT TTCACCGGTC CCGGTAGTGC CAGCCCCATC GAAGCCCGGA
    TCAGAAGAGT GGGTGTATGT AAATGAACCA ATTCCTGAGG AAATACCATC ATTTTTAGTA
    CCATACTGGA AATTGATAGA AAACTCCTAT ATAAATTCCA TCAAAACAGT ACTCAGGGAG
    TTGAGAGAAG GCCAGCATAG TGTACTTGCT TACTTATATG ATATTAGAAC AGGCTTCCAG
    CAGTTTCTGA AGCGTCCAGA CCACAAGCAA GACTTCGTGT CTCAGTGGCA GGCGGATTTC
    AACTCCCTTC CTGATGACCT GTGGGATGAT GAGGAAACAA AGGCTGAGCT GCACCAAAGA
    GTGAATGATT TACGGGACCG CCTGTGGGAC ATTTGCGATG CCCGTAAGGA AGAAGCAGAG
    CAGGAGCGTC TGGACATCAT TAACGAGAGC TGGTTGCAGG ACTCAATGGG AATTGCCATG
    AACCATTTCT TCTCACTGAT GCAGGCAGAA CTGAACCGTT TCCAAGATAC GAAGAGATTC
    CTGCAAGATT ATTACAGGGC CATGGAATGT AAAATTCCGA TGGATGACAC TAAGAAATTC
    ACTCGAGTCC CACTGGTCCG ACTGGATAGT AGAGATATTT TGGAAAGCCA GCTTAGAATT
    CCCCTGGTTC CTCGAAGATC CATTTCTCCA GAGTCAGCTT TATCCAAACC AAAAACAAAA
    AGCATCCTGA AGGGCAAGAT CGATTACACG CTGGAAAGTG TAGAGTTAAA CTTTGAGACT
    GATGAGAAGA TGGTGATGGA TACGTGGCAG CAGTCTTCTT TGGCAGTATC TCACATGGTG
    GCTGCTGAAA TTCATCAGAG GCTCATGGAA GAAGAAAAAG AAAACCAGCC AGCAGAAACA
    AAAGAAAAAC TTCCTCAGGC AGCTGCAAAT AAAAAAGTGA AAAAGGAGCC TCCGCCACCG
    CCACCACCAC CCAAGAAAAA GAAAGAGGAC AAAAAAGGAA AAGGTAAATC CCCACCTATG
    GCAGAAGCTA CTCCTGTAGT AGTGCCAGCA GAAGAGACTG AGGAAGTGGA ATTAAAAAAT
    GAACTGAAGC TCAGAATAAA GGAAGAGCAT CTTGCTGCCC TGCAATTTGA AGAGATAGCT
    ACACAGTTCC GACTTGAACT GATAAAGGTA AAAGCATTGG CTTTCCTGGA AGAGTTAGTA
    ATAAAGGCGG TTGATGTATA TAGACTCATG GACAAGTGGC TCGGTGAGAG GTACTTGAAT
    GAAATGGCCA GTGTGGAAAA ACTAACTGGG GTAGCTCGCT ATCACATTGA AACATCAACC
    AAAATTCAGA ATGAACTTTA TTTAGACCAA GAAGACTTCT TCATTAATGG CGATATCAAA
    GTCTTTCCAG ATCCACCTCC TCCGGTACGC CCTCCGCCTG TGGAAAAGGA GGAAAATGGC
    ACCCTGACCA TGGAGCAGCT TGACAACCTC CGGGATCAGT TCTTAGATAT AGCGCCCAAA
    GGCAGGGATC CTCCAGACAA TGTGAGTGGA TTCACCAAGA AGCAGAAAAG GCCTCAGAAG
    CTTGACCATT ACCTCCCAAA GAGGAACAAT CAATTTTTGA AAATTTGGCT TAAAAAATAC
    CCTTGGCTTG TATACGATGA GATACTAAAT CTTATGTTCT GCGGCCTGTG TCGTAAACAT
    GGAGTGACAT CAAAGGGAAA TCAGGTCAGT TTTTTTTACG GCACGGATAA CTTCAGGACT
    GAATTTCTCA ACGCGCATCA TCTGAGTGAG GCTCACGCCA AGGCGTCCCT CATGGAAGCC
    ACAAGTGGTT CTCCAGGGAG CAGAGCTACC ACGGAGCTGA TGGTGAGGAC CATGAGCAAG
    GTGACCCTTG GCAGGGTGGA GAACCTGTTC CGGTCCTGCC ACGCCATCGC CAAGACCGGA
    CGTCCCCTCA AAGACTTCCT CTGGATGTGT TCGCTGGACG ATATGAAGGG AATTGATATC
    GGCCCGGTGT TCAGAACCAG TAAATCGGCC AGAACTTTTA CCTACTTTAT TGCTGAAGTG
    GAACGGAGAA CTCTAAGAGA GAAGTTAGAA AAAAGTCAGT TCTTCTCCGT CCTCAGTGAT
    GGGATTGCAG ACAATTCAGC TGAGGAAGCT GAACTCGTAT ATGTGCAGTT TGCCCGCGCA
    GGGAGAGTGC ACTGCCAAAT CGTCGGGGTA CAGACCGTGG AGAAGAAGGA GCCCTGGGCG
    ATAAAACGTG CCATTGAGAA AACTCTGGAG AGAAATCTTC AACTTCGACT GTCGAACCAA
    GACTGGGCAA AGAAACTGGT GGGTTTCGGA AGCGATGGAG CTCCAGGTTC AGAGGGAGAA
    AACGGCAGCA CAGTGGCCCT GCTTTTAAGG GAAATCCAGC CAGGTGTGCT GCCTGTTTAC
    TGCCTTGCCC ATCACCTCGA ACTGTCTTAC CAGGCGGTGT TCCAGAGCGT TCCCCTCTAC
    AGCCGTGTGA AAGACCTTCT TCATAGCATG CACCGCTTCT ATCACGATTC TCCTGCTCAC
    AGGAGTGCTC TGGTGACTGC TTTCAAGGGC CTCCACCTTC AACCTGCGAT GCCCTCTCAA
    GTCAGAGGCC GGAGGTGGCT TCAGGGATTG CAGGCTGCCC TCCAGAACTT TCTCAAGGGA
    TATCCAGCAA TTGTCCAGCA GCTGCAGTCA ACAAAAGAAA GCAGAAGCAA CAGCAGTCAT
    CAGAAAGCCG AAGAACTCCT GGAGTCTCTC CTCCAGGCAG ACATTGTCAA ATTTGTCCAC
    TTCTTGGTGG ATGTCGTCAA TGTCCTTAGC ATACTGTCCC ATGTCAATCA GAACAGAAAT
    TCCTCAATTG CTGATGTATT TGCCACCTTA GAGTCAACAC TGGAAATGCT CCAAATTTAT
    CAGACAAGAC CAGGGCCGAA AGAACGTGGG GTAGATTCGG TCACACACTT TCATGGTAAC
    TGCCTTGGAG GAAAGGGAAA CATTTCTGAC GTGAGAAACA TTGTTTTAAC ACATCTTATC
    AAGAGGCTGC GAGGCTGTTT CAGAGATGCC AACCAAGATG TGGTGAAGGC AACCACGATT
    GGAAGCTTTA AACTGTGGCC GGCAAAAATA AATCAAGAAT TTGGAGAAAA AGAGGTGGCC
    ATCCTGATTG CACATTATGA ACCAGTATTA GAGGCAGCCA AGGTGAAAAT TGACCAAGTA
    GACACAGAAT GGAGCATGTT GAAATTGGAG ATCTACTCTA GATTTCAGAA CATTTGCAAA
    CTGACCTGGG ATTTTGTGAA TTCTGTTTAT TTACACAAGT ATCCAAATAT CCTGACACTC
    ATTGACCTCG TGCTGAGCCT GCCTGCAAGT TCAGCTGAGG CAGGACGTGG CTTTAGTCAG
    ATGAAGCGCA TCAGGTCCCA AATGCATGCC AAGGTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011952469.1
    CDS497..6655
    Translation

    Target ORF information:

    RefSeq Version XM_012097079.2
    Organism Ovis aries(sheep)
    Definition Ovis aries sperm flagellar 2 (SPEF2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012097079.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGTCGGAAA TCCTGTGCCA GTGGCTCAAC CAGGAATTGA AGGTGTCCCA GACCGTGAGT 
    CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAA TTGGAGAAGT TTTGCACAAG
    TTTGCACTTC AGGATGATTT TGCAGACTTT TCAGAAAGCA GGATTTCCAG TGCCAGACTT
    AACAATTTTT CTCGCTTGGA GCCCACACTT CACCTTTTGG GTGTGCAATT TGATCAGAAT
    GTGGCCCACA ATATCATCAC AGAGAAGCCT GGGGCTGCCA CGAAACTCTT ATATCAGTTG
    TACATTGCTC TCCAGAGGAA AAAGAAAGGT GTTCAGACTA GAGTGGAAAT GCCAACTGTA
    CAGCCCCTGA TGAGCACAAA AGTTCAAAAC ATGAAAAGCG AGGCTTTCCG AGATCAACGC
    TTGAGCAAAA GACGACAAAA TGAAATCATG GCCAAAATCC AAGCAGCTAT CATACAGATT
    CCTAAACCCA CATCAAATCG TACTCTGAAA ACATTTGACG CCCAAAAATT AATGAAAAAG
    AAAAAAGAGG CAGAGGATGT GGCCAATGAA ATTAAGAAGT TTGAAGCATT AATAAAAAAG
    GATCTCCAGG CAAAAGAAAG TGCATCCAAG ACTTCTCTAG ATACAGCAGG CCAGACAACC
    GCTGATTTGT TAAACACTTA TTCAGATGAT GACTACATTA AAAAAATCCA AAAACGCCTT
    GAAGAAGATG CTTTTGCACG AGAGCAAAGG GAGAAAAGAC GGCGGAGACT CTTAATGGAT
    CAGTTAATAG CTCATGAAGC CCAAGAGGAG GCTTATCGGG AGGAGCAGTT GATCAACCGG
    CTGATGCGAC AGTCCCAGCA GGAACGCAGG ATCGCTGTGC AGCTCATGCA CGTTAGGCAT
    GAAAAGGAAG TTTTATGGCA AAACAGAATT TTCAGAGAAA AACAATATGA GGAAAGACGG
    CTTAAAGACT TCCAGGATGC TCTTGATCGA GAAGCGGCTT TAGCCAGACA AGCCAAGATT
    GATTTTGAAG AACAAGCCCT CAGAGATAAG GAAATTCATG AGCAGATCGC CATAGAAAGA
    GCTCAAGCTC GTTACAAAAA GCATTATTCA ATATGTGCAG AAATTTTGGA TCAGATACTT
    GATCTGTCCA CTAAGGTGGC AGACTATCGA TTGTTGACAA ATAACCTGAT CCCATATAAG
    TTGATGCATG ATTGGAAGGA ACTGTTTTTC CATGGAAAAC CCATATATGA GCAAGCCTCT
    CTTAGACATT TACCAGCTAA GCTCTCTGCA GAACACCTTG TAGAAATGGA GAAAAGGAAC
    TTGCTAGATA ACAATGATTA TGAAGAATAT AAGAACATGG TTGGAGAATG GGCCTTACCA
    GAAGAAATGG TTGAAAACTT ACCACCCTCC AACAATTGCA TATTGGGCCA TGTGATTCAC
    AGACTAATTG AAAGTTCTCT TCCTCCTCAA GTTGAATCTA CAGTACCTGA GTTACCTTCA
    TTTGCTATAA AAGGATGCTT GTTGGGGAAA ACCTTTAGTG GAAAAACTAC TGCTCTAAAG
    GCTCTAGAGA AAAGCTTTCC TATTCAGGTA CTTTCTATTG ACACTCTTGT TCAAGAAGCT
    ATCCAAGCAT TTCATGACAA TGAAAGTATC AGCGGGGCCC ATGGACTTCA GAAAGCACCT
    GAAGAAAAGG TTTTACCAGT TAGGCGAAAG AGTGGTAAAG AAAACCAGGA CGTGCAAGAT
    GAAGCACTAC CAGAAAGAGA AGATAAAACC AGACTTGATA CCGATGCTAG AAAAGCAGCA
    AAAGCTGAAG AAGTCGTAGC GAGGGATAGT TTCTCTGAAC TCACTGTGCG TGCTCAGCTT
    GGTGCAAAAT CAGAAATGCT GCTGAAGAAA GGAAAGAGCA TATCTGATAT ATTGCTAGTC
    AGCATCATGG TAAATGCCAT TAATCAGATA CCTATGTATA AAGGCTGGAT CCTGGATGGC
    TTTCCAATGA CATTAAACCA AGCAAAACTT CTGGAGGAAG CACTCACAGG CTACAACAGA
    AACCTCATAG AACTGGAGGG AAAGAAATCA CAAACATCCA CATTAGCTGT TGACCCTACA
    GCTTCCAAAG AAGTGCCTCT TCCCCCTTCT GCATTTGATT TTGTCATGTT ATTAGACATT
    TCAGATAATT CCTCACTGAA TCGCATGAAT GACATCATGG CTAAAGCATT TTCAAGTGAA
    GCTCCTCGTG AAGATATCAA TCAGCGTGTA GCTGCTAAAA ACAAAGATAC AGATGAGGAC
    CAGAATTTAA GAGATCAAAT ACAGCACAGA ATTGTAGGCT TCCTGGACAA CTGGCCTTCG
    TTGGAGCAGT GGTTTACAGA GCCAGAAAAC ATTTTGATAA AAATCAATGC TGAAGTAGAT
    AAACAGTCAT TGTTCCAAAA AGTAAAGGAA ATGATTATGA CTGAAATAAT TAAAAAAGAA
    AATAAAGTGA AAAAGAAATT AGAAGAAAAG GAAGCTGAGA GAAAAGAAGA AGTTTCCCTG
    AGTGAGAAGA CATCCCCTCC TCCTCCTCCC CCTCCGGAAC CAGAGAAAGA GAAGGAAGTC
    CACCTTCCAC AGGAGCGGTC CAAAACTCCT GCTGCAAAAG GAAAGCAAGC ATCAGAACCC
    CTGCATGGTA ATCGAGAACC TCTTCAGGAA GGAAAAGGGA AGAAAAGTGA AACTTCACTC
    AAAAGAAAAG GTTCTCCTAG AGGAAAATCG CTAGGAGGGA AGATTCCACT CAAGAAATCA
    CCTGCCGAGT CTACAGATAT TTCACCGGTC CCGGTAGTGC CAGCCCCATC GAAGCCCGGA
    TCAGAAGAGT GGGTGTATGT AAATGAACCA ATTCCTGAGG AAATACCATC ATTTTTAGTA
    CCATACTGGA AATTGATAGA AAACTCCTAT ATAAATTCCA TCAAAACAGT ACTCAGGGAG
    TTGAGAGAAG GCCAGCATAG TGTACTTGCT TACTTATATG ATATTAGAAC AGGCTTCCAG
    CAGTTTCTGA AGCGTCCAGA CCACAAGCAA GACTTCGTGT CTCAGTGGCA GGCGGATTTC
    AACTCCCTTC CTGATGACCT GTGGGATGAT GAGGAAACAA AGGCTGAGCT GCACCAAAGA
    GTGAATGATT TACGGGACCG CCTGTGGGAC ATTTGCGATG CCCGTAAGGA AGAAGCAGAG
    CAGGAGCGTC TGGACATCAT TAACGAGAGC TGGTTGCAGG ACTCAATGGG AATTGCCATG
    AACCATTTCT TCTCACTGAT GCAGGCAGAA CTGAACCGTT TCCAAGATAC GAAGAGATTC
    CTGCAAGATT ATTACAGGGC CATGGAATGT AAAATTCCGA TGGATGACAC TAAGAAATTC
    ACTCGAGTCC CACTGGTCCG ACTGGATAGT AGAGATATTT TGGAAAGCCA GCTTAGAATT
    CCCCTGGTTC CTCGAAGATC CATTTCTCCA GAGTCAGCTT TATCCAAACC AAAAACAAAA
    AGCATCCTGA AGGGCAAGAT CGATTACACG CTGGAAAGTG TAGAGTTAAA CTTTGAGACT
    GATGAGAAGA TGGTGATGGA TACGTGGCAG CAGTCTTCTT TGGCAGTATC TCACATGGTG
    GCTGCTGAAA TTCATCAGAG GCTCATGGAA GAAGAAAAAG AAAACCAGCC AGCAGAAACA
    AAAGAAAAAC TTCCTCAGGC AGCTGCAAAT AAAAAAGTGA AAAAGGAGCC TCCGCCACCG
    CCACCACCAC CCAAGAAAAA GAAAGAGGAC AAAAAAGGAA AAGGTAAATC CCCACCTATG
    GCAGAAGCTA CTCCTGTAGT AGTGCCAGCA GAAGAGACTG AGGAAGTGGA ATTAAAAAAT
    GAACTGAAGC TCAGAATAAA GGAAGAGCAT CTTGCTGCCC TGCAATTTGA AGAGATAGCT
    ACACAGTTCC GACTTGAACT GATAAAGGTA AAAGCATTGG CTTTCCTGGA AGAGTTAGTA
    ATAAAGGCGG TTGATGTATA TAGACTCATG GACAAGTGGC TCGGTGAGAG GTACTTGAAT
    GAAATGGCCA GTGTGGAAAA ACTAACTGGG GTAGCTCGCT ATCACATTGA AACATCAACC
    AAAATTCAGA ATGAACTTTA TTTAGACCAA GAAGACTTCT TCATTAATGG CGATATCAAA
    GTCTTTCCAG ATCCACCTCC TCCGGTACGC CCTCCGCCTG TGGAAAAGGA GGAAAATGGC
    ACCCTGACCA TGGAGCAGCT TGACAACCTC CGGGATCAGT TCTTAGATAT AGCGCCCAAA
    GGCAGGGATC CTCCAGACAA TGTGAGTGGA TTCACCAAGA AGCAGAAAAG GCCTCAGAAG
    CTTGACCATT ACCTCCCAAA GAGGAACAAT CAATTTTTGA AAATTTGGCT TAAAAAATAC
    CCTTGGCTTG TATACGATGA GATACTAAAT CTTATGTTCT GCGGCCTGTG TCGTAAACAT
    GGAGTGACAT CAAAGGGAAA TCAGGTCAGT TTTTTTTACG GCACGGATAA CTTCAGGACT
    GAATTTCTCA ACGCGCATCA TCTGAGTGAG GCTCACGCCA AGGCGTCCCT CATGGAAGCC
    ACAAGTGGTT CTCCAGGGAG CAGAGCTACC ACGGAGCTGA TGGTGAGGAC CATGAGCAAG
    GTGACCCTTG GCAGGGTGGA GAACCTGTTC CGGTCCTGCC ACGCCATCGC CAAGACCGGA
    CGTCCCCTCA AAGACTTCCT CTGGATGTGT TCGCTGGACG ATATGAAGGG AATTGATATC
    GGCCCGGTGT TCAGAACCAG TAAATCGGCC AGAACTTTTA CCTACTTTAT TGCTGAAGTG
    GAACGGAGAA CTCTAAGAGA GAAGTTAGAA AAAAGTCAGT TCTTCTCCGT CCTCAGTGAT
    GGGATTGCAG ACAATTCAGC TGAGGAAGCT GAACTCGTAT ATGTGCAGTT TGCCCGCGCA
    GGGAGAGTGC ACTGCCAAAT CGTCGGGGTA CAGACCGTGG AGAAGAAGGA GCCCTGGGCG
    ATAAAACGTG CCATTGAGAA AACTCTGGAG AGAAATCTTC AACTTCGACT GTCGAACCAA
    GACTGGGCAA AGAAACTGGT GGGTTTCGGA AGCGATGGAG CTCCAGGTTC AGAGGGAGAA
    AACGGCAGCA CAGTGGCCCT GCTTTTAAGG GAAATCCAGC CAGGTGTGCT GCCTGTTTAC
    TGCCTTGCCC ATCACCTCGA ACTGTCTTAC CAGGCGGTGT TCCAGAGCGT TCCCCTCTAC
    AGCCGTGTGA AAGACCTTCT TCATAGCATG CACCGCTTCT ATCACGATTC TCCTGCTCAC
    AGGAGTGCTC TGGTGACTGC TTTCAAGGGC CTCCACCTTC AACCTGCGAT GCCCTCTCAA
    GTCAGAGGCC GGAGGTGGCT TCAGGGATTG CAGGCTGCCC TCCAGAACTT TCTCAAGGGA
    TATCCAGCAA TTGTCCAGCA GCTGCAGTCA ACAAAAGAAA GCAGAAGCAA CAGCAGTCAT
    CAGAAAGCCG AAGAACTCCT GGAGTCTCTC CTCCAGGCAG ACATTGTCAA ATTTGTCCAC
    TTCTTGGTGG ATGTCGTCAA TGTCCTTAGC ATACTGTCCC ATGTCAATCA GAACAGAAAT
    TCCTCAATTG CTGATGTATT TGCCACCTTA GAGTCAACAC TGGAAATGCT CCAAATTTAT
    CAGACAAGAC CAGGGCCGAA AGAACGTGGG GTAGATTCGG TCACACACTT TCATGGTAAC
    TGCCTTGGAG GAAAGGGAAA CATTTCTGAC GTGAGAAACA TTGTTTTAAC ACATCTTATC
    AAGAGGCTGC GAGGCTGTTT CAGAGATGCC AACCAAGATG TGGTGAAGGC AACCACGATT
    GGAAGCTTTA AACTGTGGCC GGCAAAAATA AATCAAGAAT TTGGAGAAAA AGAGGTGGCC
    ATCCTGATTG CACATTATGA ACCAGTATTA GAGGCAGCCA AGGTGAAAAT TGACCAAGTA
    GACACAGAAT GGAGCATGTT GAAATTGGAG ATCTACTCTA GATTTCAGAA CATTTGCAAA
    CTGACCTGGG ATTTTGTGAA TTCTGTTTAT TTACACAAGT ATCCAAATAT CCTGACACTC
    ATTGACCTCG TGCTGAGCCT GCCTGCAAGT TCAGCTGAGG CAGGACGTGG CTTTAGTCAG
    ATGAAGCGCA TCAGGTCCCA AATGCATGCC AAGGTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.