TCOF1 cDNA ORF clone, Ovis aries(sheep)

The following TCOF1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TCOF1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OSm81155 XM_027970402.1
Latest version!
Ovis aries treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OSm81155 XM_027970400.1
Latest version!
Ovis aries treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OSm81156 XM_027970403.1
Latest version!
Ovis aries treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OSm81157 XM_027970404.1
Latest version!
Ovis aries treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OSm81158 XM_027970405.1
Latest version!
Ovis aries treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OOv03145 XM_012113401.2
Latest version!
Ovis aries musimon Treacher Collins-Franceschetti syndrome 1 (TCOF1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OOv03146 XM_012113403.2
Latest version!
Ovis aries musimon Treacher Collins-Franceschetti syndrome 1 (TCOF1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OOv03146 XM_012113402.2
Latest version!
Ovis aries musimon Treacher Collins-Franceschetti syndrome 1 (TCOF1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OOv03147 XM_012113404.2
Latest version!
Ovis aries musimon Treacher Collins-Franceschetti syndrome 1 (TCOF1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OOv03148 XM_012113405.2
Latest version!
Ovis aries musimon Treacher Collins-Franceschetti syndrome 1 (TCOF1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OOv03149 XM_012113406.2
Latest version!
Ovis aries musimon Treacher Collins-Franceschetti syndrome 1 (TCOF1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OOv03150 XM_012113409.2
Latest version!
Ovis aries musimon Treacher Collins-Franceschetti syndrome 1 (TCOF1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OOv03151 XM_012113410.2
Latest version!
Ovis aries musimon Treacher Collins-Franceschetti syndrome 1 (TCOF1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OOv03152 XM_012113411.2
Latest version!
Ovis aries musimon Treacher Collins-Franceschetti syndrome 1 (TCOF1), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OOv03153 XM_012113412.2
Latest version!
Ovis aries musimon Treacher Collins-Franceschetti syndrome 1 (TCOF1), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OSm49869 XM_015096143.1
Latest version!
Ovis aries Treacher Collins-Franceschetti syndrome 1 (TCOF1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OSm81155
    Clone ID Related Accession (Same CDS sequence) XM_027970400.1 , XM_027970402.1
    Accession Version XM_027970400.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4359bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-05
    Organism Ovis aries(sheep)
    Product treacle protein isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040256.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ovis aries Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGCTGAGG CCAGGAAGCG GCGGGAGCTC CTTCCCCTGA TCTACCAGCA TCTGCTGCAG 
    GCGGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA AATTTTCCTG
    AGTCAGCCTG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACTTC GGAGATTGGC
    CGGAAACGGA AGGCAGAGGA AGATGCGGCC TTGCAGGCCA AGAGGACCCG CGTGTCAGAC
    CCCATCAGCA GCTCAGAGAG CTCGGAAGAG GAGGAGGAGG AGGAGGAGGC AGAAGCCAAA
    ACCACCAAAC CCACCCCGAG ATTAGCAGCT ACCAACTCCT CAGTCACAGG GACAGTTTCG
    TCTTCAAGTG TGAAAGAAAA AGCCAAGGCA AAGACCACGA AAGCCAGCAA GACGGTGAAC
    TCCACGACAC ACCCTGCCCC GGGAAAGGCA GTGGCCCACC TCCTCACTGG GAAGTCACCC
    CAGAAGACGA CGGGGCCCTC GGCAAATGCT GTCTTGGTGT CAGAAACTGA GGAGGAGGGC
    AGCGTGTCAG CTCTCAGAAC CGCTGCCACG CCTGGAATGG CATCTGCCGA CCAGGCTGAT
    AGCTCCAGTG AGGATACCAC CAGCTCGAGC GATGAGACGG ACTTGGAGGT GAAAGCCGCA
    GTAAAACCAC TGCAGGTCAG AGCCTCAGCC ACTGCAGTCA AGGAGTCTCC GAGAAAAAGT
    GCAGCCCCAA CTCCAGGGAA GGCAGGGGTT GTGACACCCC AAGTCAAAGG CGGTGCTGTA
    ACCCCAGCCA GCAGGGCCAA GAAGCCAGAA GAGGTCTCAG AGAGCAGCGA GGAGTCCGAT
    GAGAGTGAGG AGGAGGCCCC GGTGAAGGCC TTGGAGAAAA CCACCCCAAT CAGAGTTGCT
    CCTAGTCCTG CCAAGGGGAC CCCTGGGAAA GGGGCCACCC CAGCAGCCCC TGGGAAGGCA
    GGGCCCTTAG GGAAGCCAGA GGAGGACTCG GAGAGCAGCA GCGAGGAGTC AGACAGCGAG
    GAGGAGACAC AAGCTGTCAA GACCCCAGTG CAGGCAAAGC CCTCTGGGAA GATCCCCCAG
    GTTAAAGCTG CCTCTGCAGC ACCCACCCAG GTGCTTTCCC CTAAGAAAGG GGCCCCTCCA
    GCACCCGCTG GCAAGGCAGG GCCTGCAGCC ACCCAAGCCC AGCCCAGGAA GCAGGAGAAG
    GAGGAGAGCA GCAGCAGCAG CAGCGAGGAA TCAGACAGCG AGGGCGAGGC GCCCACGGCC
    ACGCGGCAGA CTACAAGCCT GGCTCAGGCA AAGCCCTTGG AGAAAAACTC TCAGGTCAGA
    GCTGCCTCAG CCACGGGTGC AGGACCCTCG GCGAAGGGCA CAGTCCCTGC GCCCCCTCAG
    AAGGCAAGGT CTGTGGCCGC CCAGGACGGG AAGCAGGAGG ACTCGGAGAG CAGCAGTGAG
    GAGGAGTCGG ACAGCGAGGA GGAGATGCAG AGGGCAGGGA CCTCAGCCCA GGCACAGTCC
    TCGGGGAAAG CCCTCCAGGT CAGACCTTCC TCAGGTCCCA CCAAGGGGCC CCCTCAGAAG
    GCCGGACCTG CAACCACCCA GGTCAAGGTC GAAAGGGCCG AGGAAGACTC GGAGAGCAGT
    GAGGAGGAAT CAGACAGTGA GGACGAAGCA CCTGTAGCCA AGACTCCAGC CCAGGCAAAA
    TCAACTGTGA AAACTTCTCA GATCAAGGCC TCCCCAAGGA AGGGCACCCC CATTACACCC
    ACATCTGCCA GGGTCCCCCC AGTGCAAGTC GGCACACCAT CCCCCCGGAC AGCAGCAACA
    GTGAGTACGC CCACCTGTGC ATCATCCCCA GCCATAGTCA GGCACACCCA GAAGCCAGGG
    GAGGACTCTT CAAGCAGCGA GGAGTCTGAG AGCGAGGAGG AGACAGCTCC TGCTGCAACA
    GGGGGACAGA TGAAGTCTGT AGGGAAGAGC CTCCCAGTGA AAGCTGCCTC AGCATCCACC
    AAGGGGCCCT CAGGAAAAGG GACCACCCCA GGGCCCCCTG GGAAGGCAGG ACCCCCCGTG
    GCACAGGTCA AGGCTGAGGT GCAGGAAGAC TCAGAGAGCA GTGAGGAGTC GGACAGTGAG
    GAAGCGGCTG CAGCTCCCGC ACAGGTGAAG ACTTCAGTGA AAACCCCTCA GACCAAAGCC
    AACCCTTCTG CCACCAGAGT GGCTTCAGCC AAAGCAGCAG CACCAGCTCC TGGAAAGGGG
    GGCCTTGCTG TTGCTCAAGC CAAAGTGGGG TCCCCAGCCA AGGTAACCCC AGCAGCGAGA
    GCCCCCCAGA GCAGCGCCGT CTCTGGGAGC GGCCAGGTGT CCATGCCGGC TGTGGGGAAG
    GCAGCGGCTG CAGCAGCCCA GGCCCAGCCG GGGCCTGTCG GGGGCTCACA GGAGGACTCG
    GAGAGCAGTG AGGAGGAGTC GGACAGTGAG GGAGAGGCAC CTCCTCAGGC GAAGCCCTCA
    GGAAAGACCC CCCAGGTCAA AACTGCCTCA GCCCCCACCA AGGCGTGCCC CAAGAAAGGG
    GCTGCCCCAG TACCCCCTGG GAAGGCAGGA CCCCCAGCCG CCCAGGCCAG GAGGCAGGAG
    GAGGACTCGG AGAGCAGCAG CGAGGAGGAG TCAGACAGTA ACGGGGACGT GCCAGCAGCT
    GCACCTCCAG CCCAGAAGGA GGGTAGCTCC AAAACTGCCA CAAGCAAGAC CCTGACTCCA
    GCATCCCTGG AGAAGAAAGC AGAGGAGTCC TCAACCAGCA GTGATGAGGA CCTGCCGTCG
    AACCAGGTGA TTAAAACCCC TCTGATTTTT GTCGACCCTA ATCGTAGTCC AGCTAGCCCA
    GCTGCTACCC CCACCCAAGC GCAGGCTACA GGCACCCCAA GGAAGGCTCA GGCCTTGGAG
    AGCACAGCCA GGAGCTCCTC TGAGAGCGAG GACGAGGACG TGATCCCCGC TACACAGTGC
    CCGACTCCTG CCACTAGGAC CAGCGTGGTG ACTGTGCCCA CAGCACCTCC AAAGGCGGCC
    CTCAGAGCCA GTGTGGTGGG GGCTCCCAGC AGTGAGGACA CAAGTCAGGG GTCTGAAGGC
    AAGAAGCAGG AGGCCTCAGC CACTCAGGTG ACCAAGAGGA ACCTCCCACT GACGCAGGCT
    GCCCTGAAGG TTCTTGCCCA GAAAGCCAGT GAGGCCCAGC CTCCTGCTGC CAAGACCCCG
    TCTTCAAGTG GGACTGACAG TGCTGTGGGA ACTCTCTCCA CGGTGAGTCC CCAGAGCACC
    CCTACCCAGG TCAGAATGGC CAACAAGCTC AGAAAGCCTG AGGTTCCTGC AGCTCAGCGG
    GCCACAGCTA CTCCCTCGGG GCACCCCACA CCCAAGGCTC CTGCAACCTC AGATGACAGC
    AATAGCAGCT CTTCGGGAAG CGAGGACGAA GCCAAGGGGT CCCAGGCGGC CCCGTCAGCC
    CACAGGCCAG GCTCAGCCCC CTCCAGGAGG GAGACCTTGG TGGAAGAGAC CACAACAGAG
    TCCAGTGACG ATGAGGTGGT GGCACCTTCC CAGTCTCTCC TCTCAGGTTT TGTGACTCCT
    GGGCTGACTC CAGCTGGTTC CAAGACTTCA AAGGCCACTC CCAAACCAGA CACCAGCCCC
    TCTGTTTCCT CTACTCCGGC CACCAGAGAT GCCGCAGAAG GCAATCAGGA AGTGGAGCCC
    CAGCAAGCAG CAGGCACCAT GTCCCCTAAA ACAGGCAGAA AAGAGGCTGG TGCCACACCT
    CAGAAGCCCG GGAAGGGGCC TGGGAGTACC CCGGCCTCCA TGCTGGCGCT GCAGAGCAAC
    ATCACCCAGC GCCTCCTGAG CGAGCCCTGG CCCCTGAGTG AGGCGCAGGT GCAGGCCTCG
    GTGGCAAAAG TGCTCACGGA GCTGCTGGAG CAGGAGAGGA AGAAGGCCGT GGAGACCGCC
    AAGGAAGGCA ACCGGAAGGG CCGCCTGGGC CATAAGCGGA AGCTGTCCGA AGACCAGACA
    GCCCCCAAGG CCCCCAAGAG CAAGAAAAAG AAGCAGTTGG CAGCTGCAGA TAGTGGGGAG
    CATGTGGTCT CCTCAGAAAA GGCGCCCAGG ACTGTCAAAG GGAAATCCAA GAAGGACAGA
    GCAAGTGGTG ACGCCAAGGA GAAGAAGGAG AAGGAGTCTC CCGGCTCTCA GGGGGCCAAG
    GAGAAGCCAG TAGGGGAGCT GGGGACTCCG AAGAGTGACG GGGGAGACCA CGGCAACCTT
    AAGATCAAGA AAGAGAAGAA GAAATCTGAC AAGAAAAAAA AAGACAAGGA GAAAAAGGAA
    AAGAAGAAGA AAACAAAAAA GGCCTCCACC AAAGACCCTG GCTCACCATC ACAGAAGAAA
    AAGAAGAGAA AGAAGAAGAC GGCAGAGCAG ACTGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_027826201.1
    CDS50..4408
    Translation

    Target ORF information:

    RefSeq Version XM_027970400.1
    Organism Ovis aries(sheep)
    Definition Ovis aries treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_027970400.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGCTGAGG CCAGGAAGCG GCGGGAGCTC CTTCCCCTGA TCTACCAGCA TCTGCTGCAG 
    GCGGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA AATTTTCCTG
    AGTCAGCCTG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACTTC GGAGATTGGC
    CGGAAACGGA AGGCAGAGGA AGATGCGGCC TTGCAGGCCA AGAGGACCCG CGTGTCAGAC
    CCCATCAGCA GCTCAGAGAG CTCGGAAGAG GAGGAGGAGG AGGAGGAGGC AGAAGCCAAA
    ACCACCAAAC CCACCCCGAG ATTAGCAGCT ACCAACTCCT CAGTCACAGG GACAGTTTCG
    TCTTCAAGTG TGAAAGAAAA AGCCAAGGCA AAGACCACGA AAGCCAGCAA GACGGTGAAC
    TCCACGACAC ACCCTGCCCC GGGAAAGGCA GTGGCCCACC TCCTCACTGG GAAGTCACCC
    CAGAAGACGA CGGGGCCCTC GGCAAATGCT GTCTTGGTGT CAGAAACTGA GGAGGAGGGC
    AGCGTGTCAG CTCTCAGAAC CGCTGCCACG CCTGGAATGG CATCTGCCGA CCAGGCTGAT
    AGCTCCAGTG AGGATACCAC CAGCTCGAGC GATGAGACGG ACTTGGAGGT GAAAGCCGCA
    GTAAAACCAC TGCAGGTCAG AGCCTCAGCC ACTGCAGTCA AGGAGTCTCC GAGAAAAAGT
    GCAGCCCCAA CTCCAGGGAA GGCAGGGGTT GTGACACCCC AAGTCAAAGG CGGTGCTGTA
    ACCCCAGCCA GCAGGGCCAA GAAGCCAGAA GAGGTCTCAG AGAGCAGCGA GGAGTCCGAT
    GAGAGTGAGG AGGAGGCCCC GGTGAAGGCC TTGGAGAAAA CCACCCCAAT CAGAGTTGCT
    CCTAGTCCTG CCAAGGGGAC CCCTGGGAAA GGGGCCACCC CAGCAGCCCC TGGGAAGGCA
    GGGCCCTTAG GGAAGCCAGA GGAGGACTCG GAGAGCAGCA GCGAGGAGTC AGACAGCGAG
    GAGGAGACAC AAGCTGTCAA GACCCCAGTG CAGGCAAAGC CCTCTGGGAA GATCCCCCAG
    GTTAAAGCTG CCTCTGCAGC ACCCACCCAG GTGCTTTCCC CTAAGAAAGG GGCCCCTCCA
    GCACCCGCTG GCAAGGCAGG GCCTGCAGCC ACCCAAGCCC AGCCCAGGAA GCAGGAGAAG
    GAGGAGAGCA GCAGCAGCAG CAGCGAGGAA TCAGACAGCG AGGGCGAGGC GCCCACGGCC
    ACGCGGCAGA CTACAAGCCT GGCTCAGGCA AAGCCCTTGG AGAAAAACTC TCAGGTCAGA
    GCTGCCTCAG CCACGGGTGC AGGACCCTCG GCGAAGGGCA CAGTCCCTGC GCCCCCTCAG
    AAGGCAAGGT CTGTGGCCGC CCAGGACGGG AAGCAGGAGG ACTCGGAGAG CAGCAGTGAG
    GAGGAGTCGG ACAGCGAGGA GGAGATGCAG AGGGCAGGGA CCTCAGCCCA GGCACAGTCC
    TCGGGGAAAG CCCTCCAGGT CAGACCTTCC TCAGGTCCCA CCAAGGGGCC CCCTCAGAAG
    GCCGGACCTG CAACCACCCA GGTCAAGGTC GAAAGGGCCG AGGAAGACTC GGAGAGCAGT
    GAGGAGGAAT CAGACAGTGA GGACGAAGCA CCTGTAGCCA AGACTCCAGC CCAGGCAAAA
    TCAACTGTGA AAACTTCTCA GATCAAGGCC TCCCCAAGGA AGGGCACCCC CATTACACCC
    ACATCTGCCA GGGTCCCCCC AGTGCAAGTC GGCACACCAT CCCCCCGGAC AGCAGCAACA
    GTGAGTACGC CCACCTGTGC ATCATCCCCA GCCATAGTCA GGCACACCCA GAAGCCAGGG
    GAGGACTCTT CAAGCAGCGA GGAGTCTGAG AGCGAGGAGG AGACAGCTCC TGCTGCAACA
    GGGGGACAGA TGAAGTCTGT AGGGAAGAGC CTCCCAGTGA AAGCTGCCTC AGCATCCACC
    AAGGGGCCCT CAGGAAAAGG GACCACCCCA GGGCCCCCTG GGAAGGCAGG ACCCCCCGTG
    GCACAGGTCA AGGCTGAGGT GCAGGAAGAC TCAGAGAGCA GTGAGGAGTC GGACAGTGAG
    GAAGCGGCTG CAGCTCCCGC ACAGGTGAAG ACTTCAGTGA AAACCCCTCA GACCAAAGCC
    AACCCTTCTG CCACCAGAGT GGCTTCAGCC AAAGCAGCAG CACCAGCTCC TGGAAAGGGG
    GGCCTTGCTG TTGCTCAAGC CAAAGTGGGG TCCCCAGCCA AGGTAACCCC AGCAGCGAGA
    GCCCCCCAGA GCAGCGCCGT CTCTGGGAGC GGCCAGGTGT CCATGCCGGC TGTGGGGAAG
    GCAGCGGCTG CAGCAGCCCA GGCCCAGCCG GGGCCTGTCG GGGGCTCACA GGAGGACTCG
    GAGAGCAGTG AGGAGGAGTC GGACAGTGAG GGAGAGGCAC CTCCTCAGGC GAAGCCCTCA
    GGAAAGACCC CCCAGGTCAA AACTGCCTCA GCCCCCACCA AGGCGTGCCC CAAGAAAGGG
    GCTGCCCCAG TACCCCCTGG GAAGGCAGGA CCCCCAGCCG CCCAGGCCAG GAGGCAGGAG
    GAGGACTCGG AGAGCAGCAG CGAGGAGGAG TCAGACAGTA ACGGGGACGT GCCAGCAGCT
    GCACCTCCAG CCCAGAAGGA GGGTAGCTCC AAAACTGCCA CAAGCAAGAC CCTGACTCCA
    GCATCCCTGG AGAAGAAAGC AGAGGAGTCC TCAACCAGCA GTGATGAGGA CCTGCCGTCG
    AACCAGGTGA TTAAAACCCC TCTGATTTTT GTCGACCCTA ATCGTAGTCC AGCTAGCCCA
    GCTGCTACCC CCACCCAAGC GCAGGCTACA GGCACCCCAA GGAAGGCTCA GGCCTTGGAG
    AGCACAGCCA GGAGCTCCTC TGAGAGCGAG GACGAGGACG TGATCCCCGC TACACAGTGC
    CCGACTCCTG CCACTAGGAC CAGCGTGGTG ACTGTGCCCA CAGCACCTCC AAAGGCGGCC
    CTCAGAGCCA GTGTGGTGGG GGCTCCCAGC AGTGAGGACA CAAGTCAGGG GTCTGAAGGC
    AAGAAGCAGG AGGCCTCAGC CACTCAGGTG ACCAAGAGGA ACCTCCCACT GACGCAGGCT
    GCCCTGAAGG TTCTTGCCCA GAAAGCCAGT GAGGCCCAGC CTCCTGCTGC CAAGACCCCG
    TCTTCAAGTG GGACTGACAG TGCTGTGGGA ACTCTCTCCA CGGTGAGTCC CCAGAGCACC
    CCTACCCAGG TCAGAATGGC CAACAAGCTC AGAAAGCCTG AGGTTCCTGC AGCTCAGCGG
    GCCACAGCTA CTCCCTCGGG GCACCCCACA CCCAAGGCTC CTGCAACCTC AGATGACAGC
    AATAGCAGCT CTTCGGGAAG CGAGGACGAA GCCAAGGGGT CCCAGGCGGC CCCGTCAGCC
    CACAGGCCAG GCTCAGCCCC CTCCAGGAGG GAGACCTTGG TGGAAGAGAC CACAACAGAG
    TCCAGTGACG ATGAGGTGGT GGCACCTTCC CAGTCTCTCC TCTCAGGTTT TGTGACTCCT
    GGGCTGACTC CAGCTGGTTC CAAGACTTCA AAGGCCACTC CCAAACCAGA CACCAGCCCC
    TCTGTTTCCT CTACTCCGGC CACCAGAGAT GCCGCAGAAG GCAATCAGGA AGTGGAGCCC
    CAGCAAGCAG CAGGCACCAT GTCCCCTAAA ACAGGCAGAA AAGAGGCTGG TGCCACACCT
    CAGAAGCCCG GGAAGGGGCC TGGGAGTACC CCGGCCTCCA TGCTGGCGCT GCAGAGCAAC
    ATCACCCAGC GCCTCCTGAG CGAGCCCTGG CCCCTGAGTG AGGCGCAGGT GCAGGCCTCG
    GTGGCAAAAG TGCTCACGGA GCTGCTGGAG CAGGAGAGGA AGAAGGCCGT GGAGACCGCC
    AAGGAAGGCA ACCGGAAGGG CCGCCTGGGC CATAAGCGGA AGCTGTCCGA AGACCAGACA
    GCCCCCAAGG CCCCCAAGAG CAAGAAAAAG AAGCAGTTGG CAGCTGCAGA TAGTGGGGAG
    CATGTGGTCT CCTCAGAAAA GGCGCCCAGG ACTGTCAAAG GGAAATCCAA GAAGGACAGA
    GCAAGTGGTG ACGCCAAGGA GAAGAAGGAG AAGGAGTCTC CCGGCTCTCA GGGGGCCAAG
    GAGAAGCCAG TAGGGGAGCT GGGGACTCCG AAGAGTGACG GGGGAGACCA CGGCAACCTT
    AAGATCAAGA AAGAGAAGAA GAAATCTGAC AAGAAAAAAA AAGACAAGGA GAAAAAGGAA
    AAGAAGAAGA AAACAAAAAA GGCCTCCACC AAAGACCCTG GCTCACCATC ACAGAAGAAA
    AAGAAGAGAA AGAAGAAGAC GGCAGAGCAG ACTGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OSm81155
    Clone ID Related Accession (Same CDS sequence) XM_027970400.1 , XM_027970402.1
    Accession Version XM_027970402.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4359bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-05
    Organism Ovis aries(sheep)
    Product treacle protein isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040256.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ovis aries Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGCTGAGG CCAGGAAGCG GCGGGAGCTC CTTCCCCTGA TCTACCAGCA TCTGCTGCAG 
    GCGGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA AATTTTCCTG
    AGTCAGCCTG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACTTC GGAGATTGGC
    CGGAAACGGA AGGCAGAGGA AGATGCGGCC TTGCAGGCCA AGAGGACCCG CGTGTCAGAC
    CCCATCAGCA GCTCAGAGAG CTCGGAAGAG GAGGAGGAGG AGGAGGAGGC AGAAGCCAAA
    ACCACCAAAC CCACCCCGAG ATTAGCAGCT ACCAACTCCT CAGTCACAGG GACAGTTTCG
    TCTTCAAGTG TGAAAGAAAA AGCCAAGGCA AAGACCACGA AAGCCAGCAA GACGGTGAAC
    TCCACGACAC ACCCTGCCCC GGGAAAGGCA GTGGCCCACC TCCTCACTGG GAAGTCACCC
    CAGAAGACGA CGGGGCCCTC GGCAAATGCT GTCTTGGTGT CAGAAACTGA GGAGGAGGGC
    AGCGTGTCAG CTCTCAGAAC CGCTGCCACG CCTGGAATGG CATCTGCCGA CCAGGCTGAT
    AGCTCCAGTG AGGATACCAC CAGCTCGAGC GATGAGACGG ACTTGGAGGT GAAAGCCGCA
    GTAAAACCAC TGCAGGTCAG AGCCTCAGCC ACTGCAGTCA AGGAGTCTCC GAGAAAAAGT
    GCAGCCCCAA CTCCAGGGAA GGCAGGGGTT GTGACACCCC AAGTCAAAGG CGGTGCTGTA
    ACCCCAGCCA GCAGGGCCAA GAAGCCAGAA GAGGTCTCAG AGAGCAGCGA GGAGTCCGAT
    GAGAGTGAGG AGGAGGCCCC GGTGAAGGCC TTGGAGAAAA CCACCCCAAT CAGAGTTGCT
    CCTAGTCCTG CCAAGGGGAC CCCTGGGAAA GGGGCCACCC CAGCAGCCCC TGGGAAGGCA
    GGGCCCTTAG GGAAGCCAGA GGAGGACTCG GAGAGCAGCA GCGAGGAGTC AGACAGCGAG
    GAGGAGACAC AAGCTGTCAA GACCCCAGTG CAGGCAAAGC CCTCTGGGAA GATCCCCCAG
    GTTAAAGCTG CCTCTGCAGC ACCCACCCAG GTGCTTTCCC CTAAGAAAGG GGCCCCTCCA
    GCACCCGCTG GCAAGGCAGG GCCTGCAGCC ACCCAAGCCC AGCCCAGGAA GCAGGAGAAG
    GAGGAGAGCA GCAGCAGCAG CAGCGAGGAA TCAGACAGCG AGGGCGAGGC GCCCACGGCC
    ACGCGGCAGA CTACAAGCCT GGCTCAGGCA AAGCCCTTGG AGAAAAACTC TCAGGTCAGA
    GCTGCCTCAG CCACGGGTGC AGGACCCTCG GCGAAGGGCA CAGTCCCTGC GCCCCCTCAG
    AAGGCAAGGT CTGTGGCCGC CCAGGACGGG AAGCAGGAGG ACTCGGAGAG CAGCAGTGAG
    GAGGAGTCGG ACAGCGAGGA GGAGATGCAG AGGGCAGGGA CCTCAGCCCA GGCACAGTCC
    TCGGGGAAAG CCCTCCAGGT CAGACCTTCC TCAGGTCCCA CCAAGGGGCC CCCTCAGAAG
    GCCGGACCTG CAACCACCCA GGTCAAGGTC GAAAGGGCCG AGGAAGACTC GGAGAGCAGT
    GAGGAGGAAT CAGACAGTGA GGACGAAGCA CCTGTAGCCA AGACTCCAGC CCAGGCAAAA
    TCAACTGTGA AAACTTCTCA GATCAAGGCC TCCCCAAGGA AGGGCACCCC CATTACACCC
    ACATCTGCCA GGGTCCCCCC AGTGCAAGTC GGCACACCAT CCCCCCGGAC AGCAGCAACA
    GTGAGTACGC CCACCTGTGC ATCATCCCCA GCCATAGTCA GGCACACCCA GAAGCCAGGG
    GAGGACTCTT CAAGCAGCGA GGAGTCTGAG AGCGAGGAGG AGACAGCTCC TGCTGCAACA
    GGGGGACAGA TGAAGTCTGT AGGGAAGAGC CTCCCAGTGA AAGCTGCCTC AGCATCCACC
    AAGGGGCCCT CAGGAAAAGG GACCACCCCA GGGCCCCCTG GGAAGGCAGG ACCCCCCGTG
    GCACAGGTCA AGGCTGAGGT GCAGGAAGAC TCAGAGAGCA GTGAGGAGTC GGACAGTGAG
    GAAGCGGCTG CAGCTCCCGC ACAGGTGAAG ACTTCAGTGA AAACCCCTCA GACCAAAGCC
    AACCCTTCTG CCACCAGAGT GGCTTCAGCC AAAGCAGCAG CACCAGCTCC TGGAAAGGGG
    GGCCTTGCTG TTGCTCAAGC CAAAGTGGGG TCCCCAGCCA AGGTAACCCC AGCAGCGAGA
    GCCCCCCAGA GCAGCGCCGT CTCTGGGAGC GGCCAGGTGT CCATGCCGGC TGTGGGGAAG
    GCAGCGGCTG CAGCAGCCCA GGCCCAGCCG GGGCCTGTCG GGGGCTCACA GGAGGACTCG
    GAGAGCAGTG AGGAGGAGTC GGACAGTGAG GGAGAGGCAC CTCCTCAGGC GAAGCCCTCA
    GGAAAGACCC CCCAGGTCAA AACTGCCTCA GCCCCCACCA AGGCGTGCCC CAAGAAAGGG
    GCTGCCCCAG TACCCCCTGG GAAGGCAGGA CCCCCAGCCG CCCAGGCCAG GAGGCAGGAG
    GAGGACTCGG AGAGCAGCAG CGAGGAGGAG TCAGACAGTA ACGGGGACGT GCCAGCAGCT
    GCACCTCCAG CCCAGAAGGA GGGTAGCTCC AAAACTGCCA CAAGCAAGAC CCTGACTCCA
    GCATCCCTGG AGAAGAAAGC AGAGGAGTCC TCAACCAGCA GTGATGAGGA CCTGCCGTCG
    AACCAGGTGA TTAAAACCCC TCTGATTTTT GTCGACCCTA ATCGTAGTCC AGCTAGCCCA
    GCTGCTACCC CCACCCAAGC GCAGGCTACA GGCACCCCAA GGAAGGCTCA GGCCTTGGAG
    AGCACAGCCA GGAGCTCCTC TGAGAGCGAG GACGAGGACG TGATCCCCGC TACACAGTGC
    CCGACTCCTG CCACTAGGAC CAGCGTGGTG ACTGTGCCCA CAGCACCTCC AAAGGCGGCC
    CTCAGAGCCA GTGTGGTGGG GGCTCCCAGC AGTGAGGACA CAAGTCAGGG GTCTGAAGGC
    AAGAAGCAGG AGGCCTCAGC CACTCAGGTG ACCAAGAGGA ACCTCCCACT GACGCAGGCT
    GCCCTGAAGG TTCTTGCCCA GAAAGCCAGT GAGGCCCAGC CTCCTGCTGC CAAGACCCCG
    TCTTCAAGTG GGACTGACAG TGCTGTGGGA ACTCTCTCCA CGGTGAGTCC CCAGAGCACC
    CCTACCCAGG TCAGAATGGC CAACAAGCTC AGAAAGCCTG AGGTTCCTGC AGCTCAGCGG
    GCCACAGCTA CTCCCTCGGG GCACCCCACA CCCAAGGCTC CTGCAACCTC AGATGACAGC
    AATAGCAGCT CTTCGGGAAG CGAGGACGAA GCCAAGGGGT CCCAGGCGGC CCCGTCAGCC
    CACAGGCCAG GCTCAGCCCC CTCCAGGAGG GAGACCTTGG TGGAAGAGAC CACAACAGAG
    TCCAGTGACG ATGAGGTGGT GGCACCTTCC CAGTCTCTCC TCTCAGGTTT TGTGACTCCT
    GGGCTGACTC CAGCTGGTTC CAAGACTTCA AAGGCCACTC CCAAACCAGA CACCAGCCCC
    TCTGTTTCCT CTACTCCGGC CACCAGAGAT GCCGCAGAAG GCAATCAGGA AGTGGAGCCC
    CAGCAAGCAG CAGGCACCAT GTCCCCTAAA ACAGGCAGAA AAGAGGCTGG TGCCACACCT
    CAGAAGCCCG GGAAGGGGCC TGGGAGTACC CCGGCCTCCA TGCTGGCGCT GCAGAGCAAC
    ATCACCCAGC GCCTCCTGAG CGAGCCCTGG CCCCTGAGTG AGGCGCAGGT GCAGGCCTCG
    GTGGCAAAAG TGCTCACGGA GCTGCTGGAG CAGGAGAGGA AGAAGGCCGT GGAGACCGCC
    AAGGAAGGCA ACCGGAAGGG CCGCCTGGGC CATAAGCGGA AGCTGTCCGA AGACCAGACA
    GCCCCCAAGG CCCCCAAGAG CAAGAAAAAG AAGCAGTTGG CAGCTGCAGA TAGTGGGGAG
    CATGTGGTCT CCTCAGAAAA GGCGCCCAGG ACTGTCAAAG GGAAATCCAA GAAGGACAGA
    GCAAGTGGTG ACGCCAAGGA GAAGAAGGAG AAGGAGTCTC CCGGCTCTCA GGGGGCCAAG
    GAGAAGCCAG TAGGGGAGCT GGGGACTCCG AAGAGTGACG GGGGAGACCA CGGCAACCTT
    AAGATCAAGA AAGAGAAGAA GAAATCTGAC AAGAAAAAAA AAGACAAGGA GAAAAAGGAA
    AAGAAGAAGA AAACAAAAAA GGCCTCCACC AAAGACCCTG GCTCACCATC ACAGAAGAAA
    AAGAAGAGAA AGAAGAAGAC GGCAGAGCAG ACTGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_027826203.1
    CDS50..4408
    Translation

    Target ORF information:

    RefSeq Version XM_027970402.1
    Organism Ovis aries(sheep)
    Definition Ovis aries treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_027970402.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGCTGAGG CCAGGAAGCG GCGGGAGCTC CTTCCCCTGA TCTACCAGCA TCTGCTGCAG 
    GCGGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA AATTTTCCTG
    AGTCAGCCTG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACTTC GGAGATTGGC
    CGGAAACGGA AGGCAGAGGA AGATGCGGCC TTGCAGGCCA AGAGGACCCG CGTGTCAGAC
    CCCATCAGCA GCTCAGAGAG CTCGGAAGAG GAGGAGGAGG AGGAGGAGGC AGAAGCCAAA
    ACCACCAAAC CCACCCCGAG ATTAGCAGCT ACCAACTCCT CAGTCACAGG GACAGTTTCG
    TCTTCAAGTG TGAAAGAAAA AGCCAAGGCA AAGACCACGA AAGCCAGCAA GACGGTGAAC
    TCCACGACAC ACCCTGCCCC GGGAAAGGCA GTGGCCCACC TCCTCACTGG GAAGTCACCC
    CAGAAGACGA CGGGGCCCTC GGCAAATGCT GTCTTGGTGT CAGAAACTGA GGAGGAGGGC
    AGCGTGTCAG CTCTCAGAAC CGCTGCCACG CCTGGAATGG CATCTGCCGA CCAGGCTGAT
    AGCTCCAGTG AGGATACCAC CAGCTCGAGC GATGAGACGG ACTTGGAGGT GAAAGCCGCA
    GTAAAACCAC TGCAGGTCAG AGCCTCAGCC ACTGCAGTCA AGGAGTCTCC GAGAAAAAGT
    GCAGCCCCAA CTCCAGGGAA GGCAGGGGTT GTGACACCCC AAGTCAAAGG CGGTGCTGTA
    ACCCCAGCCA GCAGGGCCAA GAAGCCAGAA GAGGTCTCAG AGAGCAGCGA GGAGTCCGAT
    GAGAGTGAGG AGGAGGCCCC GGTGAAGGCC TTGGAGAAAA CCACCCCAAT CAGAGTTGCT
    CCTAGTCCTG CCAAGGGGAC CCCTGGGAAA GGGGCCACCC CAGCAGCCCC TGGGAAGGCA
    GGGCCCTTAG GGAAGCCAGA GGAGGACTCG GAGAGCAGCA GCGAGGAGTC AGACAGCGAG
    GAGGAGACAC AAGCTGTCAA GACCCCAGTG CAGGCAAAGC CCTCTGGGAA GATCCCCCAG
    GTTAAAGCTG CCTCTGCAGC ACCCACCCAG GTGCTTTCCC CTAAGAAAGG GGCCCCTCCA
    GCACCCGCTG GCAAGGCAGG GCCTGCAGCC ACCCAAGCCC AGCCCAGGAA GCAGGAGAAG
    GAGGAGAGCA GCAGCAGCAG CAGCGAGGAA TCAGACAGCG AGGGCGAGGC GCCCACGGCC
    ACGCGGCAGA CTACAAGCCT GGCTCAGGCA AAGCCCTTGG AGAAAAACTC TCAGGTCAGA
    GCTGCCTCAG CCACGGGTGC AGGACCCTCG GCGAAGGGCA CAGTCCCTGC GCCCCCTCAG
    AAGGCAAGGT CTGTGGCCGC CCAGGACGGG AAGCAGGAGG ACTCGGAGAG CAGCAGTGAG
    GAGGAGTCGG ACAGCGAGGA GGAGATGCAG AGGGCAGGGA CCTCAGCCCA GGCACAGTCC
    TCGGGGAAAG CCCTCCAGGT CAGACCTTCC TCAGGTCCCA CCAAGGGGCC CCCTCAGAAG
    GCCGGACCTG CAACCACCCA GGTCAAGGTC GAAAGGGCCG AGGAAGACTC GGAGAGCAGT
    GAGGAGGAAT CAGACAGTGA GGACGAAGCA CCTGTAGCCA AGACTCCAGC CCAGGCAAAA
    TCAACTGTGA AAACTTCTCA GATCAAGGCC TCCCCAAGGA AGGGCACCCC CATTACACCC
    ACATCTGCCA GGGTCCCCCC AGTGCAAGTC GGCACACCAT CCCCCCGGAC AGCAGCAACA
    GTGAGTACGC CCACCTGTGC ATCATCCCCA GCCATAGTCA GGCACACCCA GAAGCCAGGG
    GAGGACTCTT CAAGCAGCGA GGAGTCTGAG AGCGAGGAGG AGACAGCTCC TGCTGCAACA
    GGGGGACAGA TGAAGTCTGT AGGGAAGAGC CTCCCAGTGA AAGCTGCCTC AGCATCCACC
    AAGGGGCCCT CAGGAAAAGG GACCACCCCA GGGCCCCCTG GGAAGGCAGG ACCCCCCGTG
    GCACAGGTCA AGGCTGAGGT GCAGGAAGAC TCAGAGAGCA GTGAGGAGTC GGACAGTGAG
    GAAGCGGCTG CAGCTCCCGC ACAGGTGAAG ACTTCAGTGA AAACCCCTCA GACCAAAGCC
    AACCCTTCTG CCACCAGAGT GGCTTCAGCC AAAGCAGCAG CACCAGCTCC TGGAAAGGGG
    GGCCTTGCTG TTGCTCAAGC CAAAGTGGGG TCCCCAGCCA AGGTAACCCC AGCAGCGAGA
    GCCCCCCAGA GCAGCGCCGT CTCTGGGAGC GGCCAGGTGT CCATGCCGGC TGTGGGGAAG
    GCAGCGGCTG CAGCAGCCCA GGCCCAGCCG GGGCCTGTCG GGGGCTCACA GGAGGACTCG
    GAGAGCAGTG AGGAGGAGTC GGACAGTGAG GGAGAGGCAC CTCCTCAGGC GAAGCCCTCA
    GGAAAGACCC CCCAGGTCAA AACTGCCTCA GCCCCCACCA AGGCGTGCCC CAAGAAAGGG
    GCTGCCCCAG TACCCCCTGG GAAGGCAGGA CCCCCAGCCG CCCAGGCCAG GAGGCAGGAG
    GAGGACTCGG AGAGCAGCAG CGAGGAGGAG TCAGACAGTA ACGGGGACGT GCCAGCAGCT
    GCACCTCCAG CCCAGAAGGA GGGTAGCTCC AAAACTGCCA CAAGCAAGAC CCTGACTCCA
    GCATCCCTGG AGAAGAAAGC AGAGGAGTCC TCAACCAGCA GTGATGAGGA CCTGCCGTCG
    AACCAGGTGA TTAAAACCCC TCTGATTTTT GTCGACCCTA ATCGTAGTCC AGCTAGCCCA
    GCTGCTACCC CCACCCAAGC GCAGGCTACA GGCACCCCAA GGAAGGCTCA GGCCTTGGAG
    AGCACAGCCA GGAGCTCCTC TGAGAGCGAG GACGAGGACG TGATCCCCGC TACACAGTGC
    CCGACTCCTG CCACTAGGAC CAGCGTGGTG ACTGTGCCCA CAGCACCTCC AAAGGCGGCC
    CTCAGAGCCA GTGTGGTGGG GGCTCCCAGC AGTGAGGACA CAAGTCAGGG GTCTGAAGGC
    AAGAAGCAGG AGGCCTCAGC CACTCAGGTG ACCAAGAGGA ACCTCCCACT GACGCAGGCT
    GCCCTGAAGG TTCTTGCCCA GAAAGCCAGT GAGGCCCAGC CTCCTGCTGC CAAGACCCCG
    TCTTCAAGTG GGACTGACAG TGCTGTGGGA ACTCTCTCCA CGGTGAGTCC CCAGAGCACC
    CCTACCCAGG TCAGAATGGC CAACAAGCTC AGAAAGCCTG AGGTTCCTGC AGCTCAGCGG
    GCCACAGCTA CTCCCTCGGG GCACCCCACA CCCAAGGCTC CTGCAACCTC AGATGACAGC
    AATAGCAGCT CTTCGGGAAG CGAGGACGAA GCCAAGGGGT CCCAGGCGGC CCCGTCAGCC
    CACAGGCCAG GCTCAGCCCC CTCCAGGAGG GAGACCTTGG TGGAAGAGAC CACAACAGAG
    TCCAGTGACG ATGAGGTGGT GGCACCTTCC CAGTCTCTCC TCTCAGGTTT TGTGACTCCT
    GGGCTGACTC CAGCTGGTTC CAAGACTTCA AAGGCCACTC CCAAACCAGA CACCAGCCCC
    TCTGTTTCCT CTACTCCGGC CACCAGAGAT GCCGCAGAAG GCAATCAGGA AGTGGAGCCC
    CAGCAAGCAG CAGGCACCAT GTCCCCTAAA ACAGGCAGAA AAGAGGCTGG TGCCACACCT
    CAGAAGCCCG GGAAGGGGCC TGGGAGTACC CCGGCCTCCA TGCTGGCGCT GCAGAGCAAC
    ATCACCCAGC GCCTCCTGAG CGAGCCCTGG CCCCTGAGTG AGGCGCAGGT GCAGGCCTCG
    GTGGCAAAAG TGCTCACGGA GCTGCTGGAG CAGGAGAGGA AGAAGGCCGT GGAGACCGCC
    AAGGAAGGCA ACCGGAAGGG CCGCCTGGGC CATAAGCGGA AGCTGTCCGA AGACCAGACA
    GCCCCCAAGG CCCCCAAGAG CAAGAAAAAG AAGCAGTTGG CAGCTGCAGA TAGTGGGGAG
    CATGTGGTCT CCTCAGAAAA GGCGCCCAGG ACTGTCAAAG GGAAATCCAA GAAGGACAGA
    GCAAGTGGTG ACGCCAAGGA GAAGAAGGAG AAGGAGTCTC CCGGCTCTCA GGGGGCCAAG
    GAGAAGCCAG TAGGGGAGCT GGGGACTCCG AAGAGTGACG GGGGAGACCA CGGCAACCTT
    AAGATCAAGA AAGAGAAGAA GAAATCTGAC AAGAAAAAAA AAGACAAGGA GAAAAAGGAA
    AAGAAGAAGA AAACAAAAAA GGCCTCCACC AAAGACCCTG GCTCACCATC ACAGAAGAAA
    AAGAAGAGAA AGAAGAAGAC GGCAGAGCAG ACTGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OSm81156
    Clone ID Related Accession (Same CDS sequence) XM_027970403.1
    Accession Version XM_027970403.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4230bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-05
    Organism Ovis aries(sheep)
    Product treacle protein isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040256.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ovis aries Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGCTGAGG CCAGGAAGCG GCGGGAGCTC CTTCCCCTGA TCTACCAGCA TCTGCTGCAG 
    GCGGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA AATTTTCCTG
    AGTCAGCCTG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACTTC GGAGATTGGC
    CGGAAACGGA AGGCAGAGGA AGATGCGGCC TTGCAGGCCA AGAGGACCCG CGTGTCAGAC
    CCCATCAGCA GCTCAGAGAG CTCGGAAGAG GAGGAGGAGG AGGAGGAGGC AGAAGCCAAA
    ACCACCAAAC CCACCCCGAG ATTAGCAGCT ACCAACTCCT CAGTCACAGG GACAGTTTCG
    TCTTCAAGTG TGAAAGAAAA AGCCAAGGCA AAGACCACGA AAGCCAGCAA GACGGTGAAC
    TCCACGACAC ACCCTGCCCC GGGAAAGGCA GTGGCCCACC TCCTCACTGG GAAGTCACCC
    CAGAAGACGA CGGGGCCCTC GGCAAATGCT GTCTTGGTGT CAGAAACTGA GGAGGAGGGC
    AGCGTGTCAG CTCTCAGAAC CGCTGCCACG CCTGGAATGG CATCTGCCGA CCAGGCTGAT
    AGCTCCAGTG AGGATACCAC CAGCTCGAGC GATGAGACGG ACTTGGAGGT GAAAGCCGCA
    GTAAAACCAC TGCAGGTCAG AGCCTCAGCC ACTGCAGTCA AGGAGTCTCC GAGAAAAAGT
    GCAGCCCCAA CTCCAGGGAA GGCAGGGGTT GTGACACCCC AAGTCAAAGG CGGTGCTGTA
    ACCCCAGCCA GCAGGGCCAA GAAGCCAGAA GAGGTCTCAG AGAGCAGCGA GGAGTCCGAT
    GAGAGTGAGG AGGAGGCCCC GGTGAAGGCC TTGGAGAAAA CCACCCCAAT CAGAGTTGCT
    CCTAGTCCTG CCAAGGGGAC CCCTGGGAAA GGGGCCACCC CAGCAGCCCC TGGGAAGGCA
    GGGCCCTTAG GGAAGCCAGA GGAGGACTCG GAGAGCAGCA GCGAGGAGTC AGACAGCGAG
    GAGGAGACAC AAGCTGTCAA GACCCCAGTG CAGGCAAAGC CCTCTGGGAA GATCCCCCAG
    GTTAAAGCTG CCTCTGCAGC ACCCACCCAG GTGCTTTCCC CTAAGAAAGG GGCCCCTCCA
    GCACCCGCTG GCAAGGCAGG GCCTGCAGCC ACCCAAGCCC AGCCCAGGAA GCAGGAGAAG
    GAGGAGAGCA GCAGCAGCAG CAGCGAGGAA TCAGACAGCG AGGGCGAGGC GCCCACGGCC
    ACGCGGCAGA CTACAAGCCT GGCTCAGGCA AAGCCCTTGG AGAAAAACTC TCAGGTCAGA
    GCTGCCTCAG CCACGGGTGC AGGACCCTCG GCGAAGGGCA CAGTCCCTGC GCCCCCTCAG
    AAGGCAAGGT CTGTGGCCGC CCAGGACGGG AAGCAGGAGG ACTCGGAGAG CAGCAGTGAG
    GAGGAGTCGG ACAGCGAGGA GGAGATGCAG AGGGCAGGGA CCTCAGCCCA GGCACAGTCC
    TCGGGGAAAG CCCTCCAGGT CAGACCTTCC TCAGGTCCCA CCAAGGGGCC CCCTCAGAAG
    GCCGGACCTG CAACCACCCA GGTCAAGGTC GAAAGGGCCG AGGAAGACTC GGAGAGCAGT
    GAGGAGGAAT CAGACAGTGA GGACGAAGCA CCTGTAGCCA AGACTCCAGC CCAGGCAAAA
    TCAACTGTGA AAACTTCTCA GATCAAGGCC TCCCCAAGGA AGGGCACCCC CATTACACCC
    ACATCTGCCA GGGTCCCCCC AGTGCAAGTC GGCACACCAT CCCCCCGGAC AGCAGCAACA
    ATGAAGTCTG TAGGGAAGAG CCTCCCAGTG AAAGCTGCCT CAGCATCCAC CAAGGGGCCC
    TCAGGAAAAG GGACCACCCC AGGGCCCCCT GGGAAGGCAG GACCCCCCGT GGCACAGGTC
    AAGGCTGAGG TGCAGGAAGA CTCAGAGAGC AGTGAGGAGT CGGACAGTGA GGAAGCGGCT
    GCAGCTCCCG CACAGGTGAA GACTTCAGTG AAAACCCCTC AGACCAAAGC CAACCCTTCT
    GCCACCAGAG TGGCTTCAGC CAAAGCAGCA GCACCAGCTC CTGGAAAGGG GGGCCTTGCT
    GTTGCTCAAG CCAAAGTGGG GTCCCCAGCC AAGGTAACCC CAGCAGCGAG AGCCCCCCAG
    AGCAGCGCCG TCTCTGGGAG CGGCCAGGTG TCCATGCCGG CTGTGGGGAA GGCAGCGGCT
    GCAGCAGCCC AGGCCCAGCC GGGGCCTGTC GGGGGCTCAC AGGAGGACTC GGAGAGCAGT
    GAGGAGGAGT CGGACAGTGA GGGAGAGGCA CCTCCTCAGG CGAAGCCCTC AGGAAAGACC
    CCCCAGGTCA AAACTGCCTC AGCCCCCACC AAGGCGTGCC CCAAGAAAGG GGCTGCCCCA
    GTACCCCCTG GGAAGGCAGG ACCCCCAGCC GCCCAGGCCA GGAGGCAGGA GGAGGACTCG
    GAGAGCAGCA GCGAGGAGGA GTCAGACAGT AACGGGGACG TGCCAGCAGC TGCACCTCCA
    GCCCAGAAGG AGGGTAGCTC CAAAACTGCC ACAAGCAAGA CCCTGACTCC AGCATCCCTG
    GAGAAGAAAG CAGAGGAGTC CTCAACCAGC AGTGATGAGG ACCTGCCGTC GAACCAGGTG
    ATTAAAACCC CTCTGATTTT TGTCGACCCT AATCGTAGTC CAGCTAGCCC AGCTGCTACC
    CCCACCCAAG CGCAGGCTAC AGGCACCCCA AGGAAGGCTC AGGCCTTGGA GAGCACAGCC
    AGGAGCTCCT CTGAGAGCGA GGACGAGGAC GTGATCCCCG CTACACAGTG CCCGACTCCT
    GCCACTAGGA CCAGCGTGGT GACTGTGCCC ACAGCACCTC CAAAGGCGGC CCTCAGAGCC
    AGTGTGGTGG GGGCTCCCAG CAGTGAGGAC ACAAGTCAGG GGTCTGAAGG CAAGAAGCAG
    GAGGCCTCAG CCACTCAGGT GACCAAGAGG AACCTCCCAC TGACGCAGGC TGCCCTGAAG
    GTTCTTGCCC AGAAAGCCAG TGAGGCCCAG CCTCCTGCTG CCAAGACCCC GTCTTCAAGT
    GGGACTGACA GTGCTGTGGG AACTCTCTCC ACGGTGAGTC CCCAGAGCAC CCCTACCCAG
    GTCAGAATGG CCAACAAGCT CAGAAAGCCT GAGGTTCCTG CAGCTCAGCG GGCCACAGCT
    ACTCCCTCGG GGCACCCCAC ACCCAAGGCT CCTGCAACCT CAGATGACAG CAATAGCAGC
    TCTTCGGGAA GCGAGGACGA AGCCAAGGGG TCCCAGGCGG CCCCGTCAGC CCACAGGCCA
    GGCTCAGCCC CCTCCAGGAG GGAGACCTTG GTGGAAGAGA CCACAACAGA GTCCAGTGAC
    GATGAGGTGG TGGCACCTTC CCAGTCTCTC CTCTCAGGTT TTGTGACTCC TGGGCTGACT
    CCAGCTGGTT CCAAGACTTC AAAGGCCACT CCCAAACCAG ACACCAGCCC CTCTGTTTCC
    TCTACTCCGG CCACCAGAGA TGCCGCAGAA GGCAATCAGG AAGTGGAGCC CCAGCAAGCA
    GCAGGCACCA TGTCCCCTAA AACAGGCAGA AAAGAGGCTG GTGCCACACC TCAGAAGCCC
    GGGAAGGGGC CTGGGAGTAC CCCGGCCTCC ATGCTGGCGC TGCAGAGCAA CATCACCCAG
    CGCCTCCTGA GCGAGCCCTG GCCCCTGAGT GAGGCGCAGG TGCAGGCCTC GGTGGCAAAA
    GTGCTCACGG AGCTGCTGGA GCAGGAGAGG AAGAAGGCCG TGGAGACCGC CAAGGAAGGC
    AACCGGAAGG GCCGCCTGGG CCATAAGCGG AAGCTGTCCG AAGACCAGAC AGCCCCCAAG
    GCCCCCAAGA GCAAGAAAAA GAAGCAGTTG GCAGCTGCAG ATAGTGGGGA GCATGTGGTC
    TCCTCAGAAA AGGCGCCCAG GACTGTCAAA GGGAAATCCA AGAAGGACAG AGCAAGTGGT
    GACGCCAAGG AGAAGAAGGA GAAGGAGTCT CCCGGCTCTC AGGGGGCCAA GGAGAAGCCA
    GTAGGGGAGC TGGGGACTCC GAAGAGTGAC GGGGGAGACC ACGGCAACCT TAAGATCAAG
    AAAGAGAAGA AGAAATCTGA CAAGAAAAAA AAAGACAAGG AGAAAAAGGA AAAGAAGAAG
    AAAACAAAAA AGGCCTCCAC CAAAGACCCT GGCTCACCAT CACAGAAGAA AAAGAAGAGA
    AAGAAGAAGA CGGCAGAGCA GACTGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_027826204.1
    CDS50..4279
    Translation

    Target ORF information:

    RefSeq Version XM_027970403.1
    Organism Ovis aries(sheep)
    Definition Ovis aries treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_027970403.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGCTGAGG CCAGGAAGCG GCGGGAGCTC CTTCCCCTGA TCTACCAGCA TCTGCTGCAG 
    GCGGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA AATTTTCCTG
    AGTCAGCCTG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACTTC GGAGATTGGC
    CGGAAACGGA AGGCAGAGGA AGATGCGGCC TTGCAGGCCA AGAGGACCCG CGTGTCAGAC
    CCCATCAGCA GCTCAGAGAG CTCGGAAGAG GAGGAGGAGG AGGAGGAGGC AGAAGCCAAA
    ACCACCAAAC CCACCCCGAG ATTAGCAGCT ACCAACTCCT CAGTCACAGG GACAGTTTCG
    TCTTCAAGTG TGAAAGAAAA AGCCAAGGCA AAGACCACGA AAGCCAGCAA GACGGTGAAC
    TCCACGACAC ACCCTGCCCC GGGAAAGGCA GTGGCCCACC TCCTCACTGG GAAGTCACCC
    CAGAAGACGA CGGGGCCCTC GGCAAATGCT GTCTTGGTGT CAGAAACTGA GGAGGAGGGC
    AGCGTGTCAG CTCTCAGAAC CGCTGCCACG CCTGGAATGG CATCTGCCGA CCAGGCTGAT
    AGCTCCAGTG AGGATACCAC CAGCTCGAGC GATGAGACGG ACTTGGAGGT GAAAGCCGCA
    GTAAAACCAC TGCAGGTCAG AGCCTCAGCC ACTGCAGTCA AGGAGTCTCC GAGAAAAAGT
    GCAGCCCCAA CTCCAGGGAA GGCAGGGGTT GTGACACCCC AAGTCAAAGG CGGTGCTGTA
    ACCCCAGCCA GCAGGGCCAA GAAGCCAGAA GAGGTCTCAG AGAGCAGCGA GGAGTCCGAT
    GAGAGTGAGG AGGAGGCCCC GGTGAAGGCC TTGGAGAAAA CCACCCCAAT CAGAGTTGCT
    CCTAGTCCTG CCAAGGGGAC CCCTGGGAAA GGGGCCACCC CAGCAGCCCC TGGGAAGGCA
    GGGCCCTTAG GGAAGCCAGA GGAGGACTCG GAGAGCAGCA GCGAGGAGTC AGACAGCGAG
    GAGGAGACAC AAGCTGTCAA GACCCCAGTG CAGGCAAAGC CCTCTGGGAA GATCCCCCAG
    GTTAAAGCTG CCTCTGCAGC ACCCACCCAG GTGCTTTCCC CTAAGAAAGG GGCCCCTCCA
    GCACCCGCTG GCAAGGCAGG GCCTGCAGCC ACCCAAGCCC AGCCCAGGAA GCAGGAGAAG
    GAGGAGAGCA GCAGCAGCAG CAGCGAGGAA TCAGACAGCG AGGGCGAGGC GCCCACGGCC
    ACGCGGCAGA CTACAAGCCT GGCTCAGGCA AAGCCCTTGG AGAAAAACTC TCAGGTCAGA
    GCTGCCTCAG CCACGGGTGC AGGACCCTCG GCGAAGGGCA CAGTCCCTGC GCCCCCTCAG
    AAGGCAAGGT CTGTGGCCGC CCAGGACGGG AAGCAGGAGG ACTCGGAGAG CAGCAGTGAG
    GAGGAGTCGG ACAGCGAGGA GGAGATGCAG AGGGCAGGGA CCTCAGCCCA GGCACAGTCC
    TCGGGGAAAG CCCTCCAGGT CAGACCTTCC TCAGGTCCCA CCAAGGGGCC CCCTCAGAAG
    GCCGGACCTG CAACCACCCA GGTCAAGGTC GAAAGGGCCG AGGAAGACTC GGAGAGCAGT
    GAGGAGGAAT CAGACAGTGA GGACGAAGCA CCTGTAGCCA AGACTCCAGC CCAGGCAAAA
    TCAACTGTGA AAACTTCTCA GATCAAGGCC TCCCCAAGGA AGGGCACCCC CATTACACCC
    ACATCTGCCA GGGTCCCCCC AGTGCAAGTC GGCACACCAT CCCCCCGGAC AGCAGCAACA
    ATGAAGTCTG TAGGGAAGAG CCTCCCAGTG AAAGCTGCCT CAGCATCCAC CAAGGGGCCC
    TCAGGAAAAG GGACCACCCC AGGGCCCCCT GGGAAGGCAG GACCCCCCGT GGCACAGGTC
    AAGGCTGAGG TGCAGGAAGA CTCAGAGAGC AGTGAGGAGT CGGACAGTGA GGAAGCGGCT
    GCAGCTCCCG CACAGGTGAA GACTTCAGTG AAAACCCCTC AGACCAAAGC CAACCCTTCT
    GCCACCAGAG TGGCTTCAGC CAAAGCAGCA GCACCAGCTC CTGGAAAGGG GGGCCTTGCT
    GTTGCTCAAG CCAAAGTGGG GTCCCCAGCC AAGGTAACCC CAGCAGCGAG AGCCCCCCAG
    AGCAGCGCCG TCTCTGGGAG CGGCCAGGTG TCCATGCCGG CTGTGGGGAA GGCAGCGGCT
    GCAGCAGCCC AGGCCCAGCC GGGGCCTGTC GGGGGCTCAC AGGAGGACTC GGAGAGCAGT
    GAGGAGGAGT CGGACAGTGA GGGAGAGGCA CCTCCTCAGG CGAAGCCCTC AGGAAAGACC
    CCCCAGGTCA AAACTGCCTC AGCCCCCACC AAGGCGTGCC CCAAGAAAGG GGCTGCCCCA
    GTACCCCCTG GGAAGGCAGG ACCCCCAGCC GCCCAGGCCA GGAGGCAGGA GGAGGACTCG
    GAGAGCAGCA GCGAGGAGGA GTCAGACAGT AACGGGGACG TGCCAGCAGC TGCACCTCCA
    GCCCAGAAGG AGGGTAGCTC CAAAACTGCC ACAAGCAAGA CCCTGACTCC AGCATCCCTG
    GAGAAGAAAG CAGAGGAGTC CTCAACCAGC AGTGATGAGG ACCTGCCGTC GAACCAGGTG
    ATTAAAACCC CTCTGATTTT TGTCGACCCT AATCGTAGTC CAGCTAGCCC AGCTGCTACC
    CCCACCCAAG CGCAGGCTAC AGGCACCCCA AGGAAGGCTC AGGCCTTGGA GAGCACAGCC
    AGGAGCTCCT CTGAGAGCGA GGACGAGGAC GTGATCCCCG CTACACAGTG CCCGACTCCT
    GCCACTAGGA CCAGCGTGGT GACTGTGCCC ACAGCACCTC CAAAGGCGGC CCTCAGAGCC
    AGTGTGGTGG GGGCTCCCAG CAGTGAGGAC ACAAGTCAGG GGTCTGAAGG CAAGAAGCAG
    GAGGCCTCAG CCACTCAGGT GACCAAGAGG AACCTCCCAC TGACGCAGGC TGCCCTGAAG
    GTTCTTGCCC AGAAAGCCAG TGAGGCCCAG CCTCCTGCTG CCAAGACCCC GTCTTCAAGT
    GGGACTGACA GTGCTGTGGG AACTCTCTCC ACGGTGAGTC CCCAGAGCAC CCCTACCCAG
    GTCAGAATGG CCAACAAGCT CAGAAAGCCT GAGGTTCCTG CAGCTCAGCG GGCCACAGCT
    ACTCCCTCGG GGCACCCCAC ACCCAAGGCT CCTGCAACCT CAGATGACAG CAATAGCAGC
    TCTTCGGGAA GCGAGGACGA AGCCAAGGGG TCCCAGGCGG CCCCGTCAGC CCACAGGCCA
    GGCTCAGCCC CCTCCAGGAG GGAGACCTTG GTGGAAGAGA CCACAACAGA GTCCAGTGAC
    GATGAGGTGG TGGCACCTTC CCAGTCTCTC CTCTCAGGTT TTGTGACTCC TGGGCTGACT
    CCAGCTGGTT CCAAGACTTC AAAGGCCACT CCCAAACCAG ACACCAGCCC CTCTGTTTCC
    TCTACTCCGG CCACCAGAGA TGCCGCAGAA GGCAATCAGG AAGTGGAGCC CCAGCAAGCA
    GCAGGCACCA TGTCCCCTAA AACAGGCAGA AAAGAGGCTG GTGCCACACC TCAGAAGCCC
    GGGAAGGGGC CTGGGAGTAC CCCGGCCTCC ATGCTGGCGC TGCAGAGCAA CATCACCCAG
    CGCCTCCTGA GCGAGCCCTG GCCCCTGAGT GAGGCGCAGG TGCAGGCCTC GGTGGCAAAA
    GTGCTCACGG AGCTGCTGGA GCAGGAGAGG AAGAAGGCCG TGGAGACCGC CAAGGAAGGC
    AACCGGAAGG GCCGCCTGGG CCATAAGCGG AAGCTGTCCG AAGACCAGAC AGCCCCCAAG
    GCCCCCAAGA GCAAGAAAAA GAAGCAGTTG GCAGCTGCAG ATAGTGGGGA GCATGTGGTC
    TCCTCAGAAA AGGCGCCCAG GACTGTCAAA GGGAAATCCA AGAAGGACAG AGCAAGTGGT
    GACGCCAAGG AGAAGAAGGA GAAGGAGTCT CCCGGCTCTC AGGGGGCCAA GGAGAAGCCA
    GTAGGGGAGC TGGGGACTCC GAAGAGTGAC GGGGGAGACC ACGGCAACCT TAAGATCAAG
    AAAGAGAAGA AGAAATCTGA CAAGAAAAAA AAAGACAAGG AGAAAAAGGA AAAGAAGAAG
    AAAACAAAAA AGGCCTCCAC CAAAGACCCT GGCTCACCAT CACAGAAGAA AAAGAAGAGA
    AAGAAGAAGA CGGCAGAGCA GACTGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OSm81157
    Clone ID Related Accession (Same CDS sequence) XM_027970404.1
    Accession Version XM_027970404.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4038bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-05
    Organism Ovis aries(sheep)
    Product treacle protein isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040256.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ovis aries Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGCTGAGG CCAGGAAGCG GCGGGAGCTC CTTCCCCTGA TCTACCAGCA TCTGCTGCAG 
    GCGGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA AATTTTCCTG
    AGTCAGCCTG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACTTC GGAGATTGGC
    CGGAAACGGA AGGCAGAGGA AGATGCGGCC TTGCAGGCCA AGAGGACCCG CGTGTCAGAC
    CCCATCAGCA GCTCAGAGAG CTCGGAAGAG GAGGAGGAGG AGGAGGAGGC AGAAGCCAAA
    ACCACCAAAC CCACCCCGAG ATTAGCAGCT ACCAACTCCT CAGTCACAGG GACAGTTTCG
    TCTTCAAGTG TGAAAGAAAA AGCCAAGGCA AAGACCACGA AAGCCAGCAA GACGGTGAAC
    TCCACGACAC ACCCTGCCCC GGGAAAGGCA GTGGCCCACC TCCTCACTGG GAAGTCACCC
    CAGAAGACGA CGGGGCCCTC GGCAAATGCT GTCTTGGTGT CAGAAACTGA GGAGGAGGGC
    AGCGTGTCAG CTCTCAGAAC CGCTGCCACG CCTGGAATGG CATCTGCCGA CCAGGCTGAT
    AGCTCCAGTG AGGATACCAC CAGCTCGAGC GATGAGACGG ACTTGGAGGT GAAAGCCGCA
    GTAAAACCAC TGCAGGTCAG AGCCTCAGCC ACTGCAGTCA AGGAGTCTCC GAGAAAAAGT
    GCAGCCCCAA CTCCAGGGAA GGCAGGGGTT GTGACACCCC AAGTCAAAGG CGGTGCTGTA
    ACCCCAGCCA GCAGGGCCAA GAAGCCAGAA GAGGTCTCAG AGAGCAGCGA GGAGTCCGAT
    GAGAGTGAGG AGGAGGCCCC GGTGAAGGCC TTGGAGAAAA CCACCCCAAT CAGAGTTGCT
    CCTAGTCCTG CCAAGGGGAC CCCTGGGAAA GGGGCCACCC CAGCAGCCCC TGGGAAGGCA
    GGGCCCTTAG GGAAGCCAGA GGAGGACTCG GAGAGCAGCA GCGAGGAGTC AGACAGCGAG
    GAGGAGACAC AAGCTGTCAA GACCCCAGTG CAGGCAAAGC CCTCTGGGAA GATCCCCCAG
    GTTAAAGCTG CCTCTGCAGC ACCCACCCAG GTGCTTTCCC CTAAGAAAGG GGCCCCTCCA
    GCACCCGCTG GCAAGGCAGG GCCTGCAGCC ACCCAAGCCC AGCCCAGGAA GCAGGAGAAG
    GAGGAGAGCA GCAGCAGCAG CAGCGAGGAA TCAGACAGCG AGGGCGAGGC GCCCACGGCC
    ACGCGGCAGA CTACAAGCCT GGCTCAGGCA AAGCCCTTGG AGAAAAACTC TCAGGTCAGA
    GCTGCCTCAG CCACGGGTGC AGGACCCTCG GCGAAGGGCA CAGTCCCTGC GCCCCCTCAG
    AAGGCAAGGT CTGTGGCCGC CCAGGACGGG AAGCAGGAGG ACTCGGAGAG CAGCAGTGAG
    GAGGAGTCGG ACAGCGAGGA GGAGATGCAG AGGGCAGGGA CCTCAGCCCA GGCACAGTCC
    TCGGGGAAAG CCCTCCAGGT CAGACCTTCC TCAGGTCCCA CCAAGGGGCC CCCTCAGAAG
    GCCGGACCTG CAACCACCCA GGTCAAGGTC GAAAGGGCCG AGGAAGACTC GGAGAGCAGT
    GAGGAGGAAT CAGACAGTGA GGACGAAGCA CCTGTAGCCA AGACTCCAGC CCAGGCAAAA
    TCAACTGTGA AAACTTCTCA GATCAAGGCC TCCCCAAGGA AGGGCACCCC CATTACACCC
    ACATCTGCCA GGGTCCCCCC AGTGCAAGTC GGCACACCAT CCCCCCGGAC AGCAGCAACA
    GTGAGTACGC CCACCTGTGC ATCATCCCCA GCCATAGTCA GGCACACCCA GAAGCCAGGG
    GAGGACTCTT CAAGCAGCGA GGAGTCTGAG AGCGAGGAGG AGACAGCTCC TGCTGCAACA
    GGGGGACAGA TGAAGTCTGT AGGGAAGAGC CTCCCAGTGA AAGCTGCCTC AGCATCCACC
    AAGGGGCCCT CAGGAAAAGG GACCACCCCA GGGCCCCCTG GGAAGGCAGG ACCCCCCGTG
    GCACAGGTCA AGGCTGAGGT GCAGGAAGAC TCAGAGAGCA GTGAGGAGTC GGACAGTGAG
    GAAGCGGCTG CAGCTCCCGC ACAGGTGAAG ACTTCAGTGA AAACCCCTCA GACCAAAGCC
    AACCCTTCTG CCACCAGAGT GGCTTCAGCC AAAGCAGCAG CACCAGCTCC TGGAAAGGGG
    GGCCTTGCTG TTGCTCAAGC CAAAGTGGGG TCCCCAGCCA AGGTAACCCC AGCAGCGAGA
    GCCCCCCAGA GCAGCGCCGT CTCTGGGAGC GGCCAGGTGT CCATGCCGGC TGTGGGGAAG
    GCAGCGGCTG CAGCAGCCCA GGCCCAGCCG GGGCCTGTCG GGGGCTCACA GGAGGACTCG
    GAGAGCAGTG AGGAGGAGTC GGACAGTGAG GGAGAGGCAC CTCCTCAGGC GAAGCCCTCA
    GGAAAGACCC CCCAGGTCAA AACTGCCTCA GCCCCCACCA AGGCGTGCCC CAAGAAAGGG
    GCTGCCCCAG TACCCCCTGG GAAGGCAGGA CCCCCAGCCG CCCAGGCCAG GAGGCAGGAG
    GAGGACTCGG AGAGCAGCAG CGAGGAGGAG TCAGACAGTA ACGGGGACGT GCCAGCAGCT
    GCACCTCCAG CCCAGAAGGA GGGTAGCTCC AAAACTGCCA CAAGCAAGAC CCTGACTCCA
    GCATCCCTGG AGAAGAAAGC AGAGGAGTCC TCAACCAGCA GTGATGAGGA CCTGCCGTCG
    AACCAGGTGA CCAAGAGGAA CCTCCCACTG ACGCAGGCTG CCCTGAAGGT TCTTGCCCAG
    AAAGCCAGTG AGGCCCAGCC TCCTGCTGCC AAGACCCCGT CTTCAAGTGG GACTGACAGT
    GCTGTGGGAA CTCTCTCCAC GGTGAGTCCC CAGAGCACCC CTACCCAGGT CAGAATGGCC
    AACAAGCTCA GAAAGCCTGA GGTTCCTGCA GCTCAGCGGG CCACAGCTAC TCCCTCGGGG
    CACCCCACAC CCAAGGCTCC TGCAACCTCA GATGACAGCA ATAGCAGCTC TTCGGGAAGC
    GAGGACGAAG CCAAGGGGTC CCAGGCGGCC CCGTCAGCCC ACAGGCCAGG CTCAGCCCCC
    TCCAGGAGGG AGACCTTGGT GGAAGAGACC ACAACAGAGT CCAGTGACGA TGAGGTGGTG
    GCACCTTCCC AGTCTCTCCT CTCAGGTTTT GTGACTCCTG GGCTGACTCC AGCTGGTTCC
    AAGACTTCAA AGGCCACTCC CAAACCAGAC ACCAGCCCCT CTGTTTCCTC TACTCCGGCC
    ACCAGAGATG CCGCAGAAGG CAATCAGGAA GTGGAGCCCC AGCAAGCAGC AGGCACCATG
    TCCCCTAAAA CAGGCAGAAA AGAGGCTGGT GCCACACCTC AGAAGCCCGG GAAGGGGCCT
    GGGAGTACCC CGGCCTCCAT GCTGGCGCTG CAGAGCAACA TCACCCAGCG CCTCCTGAGC
    GAGCCCTGGC CCCTGAGTGA GGCGCAGGTG CAGGCCTCGG TGGCAAAAGT GCTCACGGAG
    CTGCTGGAGC AGGAGAGGAA GAAGGCCGTG GAGACCGCCA AGGAAGGCAA CCGGAAGGGC
    CGCCTGGGCC ATAAGCGGAA GCTGTCCGAA GACCAGACAG CCCCCAAGGC CCCCAAGAGC
    AAGAAAAAGA AGCAGTTGGC AGCTGCAGAT AGTGGGGAGC ATGTGGTCTC CTCAGAAAAG
    GCGCCCAGGA CTGTCAAAGG GAAATCCAAG AAGGACAGAG CAAGTGGTGA CGCCAAGGAG
    AAGAAGGAGA AGGAGTCTCC CGGCTCTCAG GGGGCCAAGG AGAAGCCAGT AGGGGAGCTG
    GGGACTCCGA AGAGTGACGG GGGAGACCAC GGCAACCTTA AGATCAAGAA AGAGAAGAAG
    AAATCTGACA AGAAAAAAAA AGACAAGGAG AAAAAGGAAA AGAAGAAGAA AACAAAAAAG
    GCCTCCACCA AAGACCCTGG CTCACCATCA CAGAAGAAAA AGAAGAGAAA GAAGAAGACG
    GCAGAGCAGA CTGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_027826205.1
    CDS50..4087
    Translation

    Target ORF information:

    RefSeq Version XM_027970404.1
    Organism Ovis aries(sheep)
    Definition Ovis aries treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_027970404.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGCTGAGG CCAGGAAGCG GCGGGAGCTC CTTCCCCTGA TCTACCAGCA TCTGCTGCAG 
    GCGGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA AATTTTCCTG
    AGTCAGCCTG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACTTC GGAGATTGGC
    CGGAAACGGA AGGCAGAGGA AGATGCGGCC TTGCAGGCCA AGAGGACCCG CGTGTCAGAC
    CCCATCAGCA GCTCAGAGAG CTCGGAAGAG GAGGAGGAGG AGGAGGAGGC AGAAGCCAAA
    ACCACCAAAC CCACCCCGAG ATTAGCAGCT ACCAACTCCT CAGTCACAGG GACAGTTTCG
    TCTTCAAGTG TGAAAGAAAA AGCCAAGGCA AAGACCACGA AAGCCAGCAA GACGGTGAAC
    TCCACGACAC ACCCTGCCCC GGGAAAGGCA GTGGCCCACC TCCTCACTGG GAAGTCACCC
    CAGAAGACGA CGGGGCCCTC GGCAAATGCT GTCTTGGTGT CAGAAACTGA GGAGGAGGGC
    AGCGTGTCAG CTCTCAGAAC CGCTGCCACG CCTGGAATGG CATCTGCCGA CCAGGCTGAT
    AGCTCCAGTG AGGATACCAC CAGCTCGAGC GATGAGACGG ACTTGGAGGT GAAAGCCGCA
    GTAAAACCAC TGCAGGTCAG AGCCTCAGCC ACTGCAGTCA AGGAGTCTCC GAGAAAAAGT
    GCAGCCCCAA CTCCAGGGAA GGCAGGGGTT GTGACACCCC AAGTCAAAGG CGGTGCTGTA
    ACCCCAGCCA GCAGGGCCAA GAAGCCAGAA GAGGTCTCAG AGAGCAGCGA GGAGTCCGAT
    GAGAGTGAGG AGGAGGCCCC GGTGAAGGCC TTGGAGAAAA CCACCCCAAT CAGAGTTGCT
    CCTAGTCCTG CCAAGGGGAC CCCTGGGAAA GGGGCCACCC CAGCAGCCCC TGGGAAGGCA
    GGGCCCTTAG GGAAGCCAGA GGAGGACTCG GAGAGCAGCA GCGAGGAGTC AGACAGCGAG
    GAGGAGACAC AAGCTGTCAA GACCCCAGTG CAGGCAAAGC CCTCTGGGAA GATCCCCCAG
    GTTAAAGCTG CCTCTGCAGC ACCCACCCAG GTGCTTTCCC CTAAGAAAGG GGCCCCTCCA
    GCACCCGCTG GCAAGGCAGG GCCTGCAGCC ACCCAAGCCC AGCCCAGGAA GCAGGAGAAG
    GAGGAGAGCA GCAGCAGCAG CAGCGAGGAA TCAGACAGCG AGGGCGAGGC GCCCACGGCC
    ACGCGGCAGA CTACAAGCCT GGCTCAGGCA AAGCCCTTGG AGAAAAACTC TCAGGTCAGA
    GCTGCCTCAG CCACGGGTGC AGGACCCTCG GCGAAGGGCA CAGTCCCTGC GCCCCCTCAG
    AAGGCAAGGT CTGTGGCCGC CCAGGACGGG AAGCAGGAGG ACTCGGAGAG CAGCAGTGAG
    GAGGAGTCGG ACAGCGAGGA GGAGATGCAG AGGGCAGGGA CCTCAGCCCA GGCACAGTCC
    TCGGGGAAAG CCCTCCAGGT CAGACCTTCC TCAGGTCCCA CCAAGGGGCC CCCTCAGAAG
    GCCGGACCTG CAACCACCCA GGTCAAGGTC GAAAGGGCCG AGGAAGACTC GGAGAGCAGT
    GAGGAGGAAT CAGACAGTGA GGACGAAGCA CCTGTAGCCA AGACTCCAGC CCAGGCAAAA
    TCAACTGTGA AAACTTCTCA GATCAAGGCC TCCCCAAGGA AGGGCACCCC CATTACACCC
    ACATCTGCCA GGGTCCCCCC AGTGCAAGTC GGCACACCAT CCCCCCGGAC AGCAGCAACA
    GTGAGTACGC CCACCTGTGC ATCATCCCCA GCCATAGTCA GGCACACCCA GAAGCCAGGG
    GAGGACTCTT CAAGCAGCGA GGAGTCTGAG AGCGAGGAGG AGACAGCTCC TGCTGCAACA
    GGGGGACAGA TGAAGTCTGT AGGGAAGAGC CTCCCAGTGA AAGCTGCCTC AGCATCCACC
    AAGGGGCCCT CAGGAAAAGG GACCACCCCA GGGCCCCCTG GGAAGGCAGG ACCCCCCGTG
    GCACAGGTCA AGGCTGAGGT GCAGGAAGAC TCAGAGAGCA GTGAGGAGTC GGACAGTGAG
    GAAGCGGCTG CAGCTCCCGC ACAGGTGAAG ACTTCAGTGA AAACCCCTCA GACCAAAGCC
    AACCCTTCTG CCACCAGAGT GGCTTCAGCC AAAGCAGCAG CACCAGCTCC TGGAAAGGGG
    GGCCTTGCTG TTGCTCAAGC CAAAGTGGGG TCCCCAGCCA AGGTAACCCC AGCAGCGAGA
    GCCCCCCAGA GCAGCGCCGT CTCTGGGAGC GGCCAGGTGT CCATGCCGGC TGTGGGGAAG
    GCAGCGGCTG CAGCAGCCCA GGCCCAGCCG GGGCCTGTCG GGGGCTCACA GGAGGACTCG
    GAGAGCAGTG AGGAGGAGTC GGACAGTGAG GGAGAGGCAC CTCCTCAGGC GAAGCCCTCA
    GGAAAGACCC CCCAGGTCAA AACTGCCTCA GCCCCCACCA AGGCGTGCCC CAAGAAAGGG
    GCTGCCCCAG TACCCCCTGG GAAGGCAGGA CCCCCAGCCG CCCAGGCCAG GAGGCAGGAG
    GAGGACTCGG AGAGCAGCAG CGAGGAGGAG TCAGACAGTA ACGGGGACGT GCCAGCAGCT
    GCACCTCCAG CCCAGAAGGA GGGTAGCTCC AAAACTGCCA CAAGCAAGAC CCTGACTCCA
    GCATCCCTGG AGAAGAAAGC AGAGGAGTCC TCAACCAGCA GTGATGAGGA CCTGCCGTCG
    AACCAGGTGA CCAAGAGGAA CCTCCCACTG ACGCAGGCTG CCCTGAAGGT TCTTGCCCAG
    AAAGCCAGTG AGGCCCAGCC TCCTGCTGCC AAGACCCCGT CTTCAAGTGG GACTGACAGT
    GCTGTGGGAA CTCTCTCCAC GGTGAGTCCC CAGAGCACCC CTACCCAGGT CAGAATGGCC
    AACAAGCTCA GAAAGCCTGA GGTTCCTGCA GCTCAGCGGG CCACAGCTAC TCCCTCGGGG
    CACCCCACAC CCAAGGCTCC TGCAACCTCA GATGACAGCA ATAGCAGCTC TTCGGGAAGC
    GAGGACGAAG CCAAGGGGTC CCAGGCGGCC CCGTCAGCCC ACAGGCCAGG CTCAGCCCCC
    TCCAGGAGGG AGACCTTGGT GGAAGAGACC ACAACAGAGT CCAGTGACGA TGAGGTGGTG
    GCACCTTCCC AGTCTCTCCT CTCAGGTTTT GTGACTCCTG GGCTGACTCC AGCTGGTTCC
    AAGACTTCAA AGGCCACTCC CAAACCAGAC ACCAGCCCCT CTGTTTCCTC TACTCCGGCC
    ACCAGAGATG CCGCAGAAGG CAATCAGGAA GTGGAGCCCC AGCAAGCAGC AGGCACCATG
    TCCCCTAAAA CAGGCAGAAA AGAGGCTGGT GCCACACCTC AGAAGCCCGG GAAGGGGCCT
    GGGAGTACCC CGGCCTCCAT GCTGGCGCTG CAGAGCAACA TCACCCAGCG CCTCCTGAGC
    GAGCCCTGGC CCCTGAGTGA GGCGCAGGTG CAGGCCTCGG TGGCAAAAGT GCTCACGGAG
    CTGCTGGAGC AGGAGAGGAA GAAGGCCGTG GAGACCGCCA AGGAAGGCAA CCGGAAGGGC
    CGCCTGGGCC ATAAGCGGAA GCTGTCCGAA GACCAGACAG CCCCCAAGGC CCCCAAGAGC
    AAGAAAAAGA AGCAGTTGGC AGCTGCAGAT AGTGGGGAGC ATGTGGTCTC CTCAGAAAAG
    GCGCCCAGGA CTGTCAAAGG GAAATCCAAG AAGGACAGAG CAAGTGGTGA CGCCAAGGAG
    AAGAAGGAGA AGGAGTCTCC CGGCTCTCAG GGGGCCAAGG AGAAGCCAGT AGGGGAGCTG
    GGGACTCCGA AGAGTGACGG GGGAGACCAC GGCAACCTTA AGATCAAGAA AGAGAAGAAG
    AAATCTGACA AGAAAAAAAA AGACAAGGAG AAAAAGGAAA AGAAGAAGAA AACAAAAAAG
    GCCTCCACCA AAGACCCTGG CTCACCATCA CAGAAGAAAA AGAAGAGAAA GAAGAAGACG
    GCAGAGCAGA CTGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OSm81158
    Clone ID Related Accession (Same CDS sequence) XM_027970405.1
    Accession Version XM_027970405.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3909bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-05
    Organism Ovis aries(sheep)
    Product treacle protein isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040256.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ovis aries Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGCTGAGG CCAGGAAGCG GCGGGAGCTC CTTCCCCTGA TCTACCAGCA TCTGCTGCAG 
    GCGGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA AATTTTCCTG
    AGTCAGCCTG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACTTC GGAGATTGGC
    CGGAAACGGA AGGCAGAGGA AGATGCGGCC TTGCAGGCCA AGAGGACCCG CGTGTCAGAC
    CCCATCAGCA GCTCAGAGAG CTCGGAAGAG GAGGAGGAGG AGGAGGAGGC AGAAGCCAAA
    ACCACCAAAC CCACCCCGAG ATTAGCAGCT ACCAACTCCT CAGTCACAGG GACAGTTTCG
    TCTTCAAGTG TGAAAGAAAA AGCCAAGGCA AAGACCACGA AAGCCAGCAA GACGGTGAAC
    TCCACGACAC ACCCTGCCCC GGGAAAGGCA GTGGCCCACC TCCTCACTGG GAAGTCACCC
    CAGAAGACGA CGGGGCCCTC GGCAAATGCT GTCTTGGTGT CAGAAACTGA GGAGGAGGGC
    AGCGTGTCAG CTCTCAGAAC CGCTGCCACG CCTGGAATGG CATCTGCCGA CCAGGCTGAT
    AGCTCCAGTG AGGATACCAC CAGCTCGAGC GATGAGACGG ACTTGGAGGT GAAAGCCGCA
    GTAAAACCAC TGCAGGTCAG AGCCTCAGCC ACTGCAGTCA AGGAGTCTCC GAGAAAAAGT
    GCAGCCCCAA CTCCAGGGAA GGCAGGGGTT GTGACACCCC AAGTCAAAGG CGGTGCTGTA
    ACCCCAGCCA GCAGGGCCAA GAAGCCAGAA GAGGTCTCAG AGAGCAGCGA GGAGTCCGAT
    GAGAGTGAGG AGGAGGCCCC GGTGAAGGCC TTGGAGAAAA CCACCCCAAT CAGAGTTGCT
    CCTAGTCCTG CCAAGGGGAC CCCTGGGAAA GGGGCCACCC CAGCAGCCCC TGGGAAGGCA
    GGGCCCTTAG GGAAGCCAGA GGAGGACTCG GAGAGCAGCA GCGAGGAGTC AGACAGCGAG
    GAGGAGACAC AAGCTGTCAA GACCCCAGTG CAGGCAAAGC CCTCTGGGAA GATCCCCCAG
    GTTAAAGCTG CCTCTGCAGC ACCCACCCAG GTGCTTTCCC CTAAGAAAGG GGCCCCTCCA
    GCACCCGCTG GCAAGGCAGG GCCTGCAGCC ACCCAAGCCC AGCCCAGGAA GCAGGAGAAG
    GAGGAGAGCA GCAGCAGCAG CAGCGAGGAA TCAGACAGCG AGGGCGAGGC GCCCACGGCC
    ACGCGGCAGA CTACAAGCCT GGCTCAGGCA AAGCCCTTGG AGAAAAACTC TCAGGTCAGA
    GCTGCCTCAG CCACGGGTGC AGGACCCTCG GCGAAGGGCA CAGTCCCTGC GCCCCCTCAG
    AAGGCAAGGT CTGTGGCCGC CCAGGACGGG AAGCAGGAGG ACTCGGAGAG CAGCAGTGAG
    GAGGAGTCGG ACAGCGAGGA GGAGATGCAG AGGGCAGGGA CCTCAGCCCA GGCACAGTCC
    TCGGGGAAAG CCCTCCAGGT CAGACCTTCC TCAGGTCCCA CCAAGGGGCC CCCTCAGAAG
    GCCGGACCTG CAACCACCCA GGTCAAGGTC GAAAGGGCCG AGGAAGACTC GGAGAGCAGT
    GAGGAGGAAT CAGACAGTGA GGACGAAGCA CCTGTAGCCA AGACTCCAGC CCAGGTGAAG
    ACTTCAGTGA AAACCCCTCA GACCAAAGCC AACCCTTCTG CCACCAGAGT GGCTTCAGCC
    AAAGCAGCAG CACCAGCTCC TGGAAAGGGG GGCCTTGCTG TTGCTCAAGC CAAAGTGGGG
    TCCCCAGCCA AGGTAACCCC AGCAGCGAGA GCCCCCCAGA GCAGCGCCGT CTCTGGGAGC
    GGCCAGGTGT CCATGCCGGC TGTGGGGAAG GCAGCGGCTG CAGCAGCCCA GGCCCAGCCG
    GGGCCTGTCG GGGGCTCACA GGAGGACTCG GAGAGCAGTG AGGAGGAGTC GGACAGTGAG
    GGAGAGGCAC CTCCTCAGGC GAAGCCCTCA GGAAAGACCC CCCAGGTCAA AACTGCCTCA
    GCCCCCACCA AGGCGTGCCC CAAGAAAGGG GCTGCCCCAG TACCCCCTGG GAAGGCAGGA
    CCCCCAGCCG CCCAGGCCAG GAGGCAGGAG GAGGACTCGG AGAGCAGCAG CGAGGAGGAG
    TCAGACAGTA ACGGGGACGT GCCAGCAGCT GCACCTCCAG CCCAGAAGGA GGGTAGCTCC
    AAAACTGCCA CAAGCAAGAC CCTGACTCCA GCATCCCTGG AGAAGAAAGC AGAGGAGTCC
    TCAACCAGCA GTGATGAGGA CCTGCCGTCG AACCAGGTGA TTAAAACCCC TCTGATTTTT
    GTCGACCCTA ATCGTAGTCC AGCTAGCCCA GCTGCTACCC CCACCCAAGC GCAGGCTACA
    GGCACCCCAA GGAAGGCTCA GGCCTTGGAG AGCACAGCCA GGAGCTCCTC TGAGAGCGAG
    GACGAGGACG TGATCCCCGC TACACAGTGC CCGACTCCTG CCACTAGGAC CAGCGTGGTG
    ACTGTGCCCA CAGCACCTCC AAAGGCGGCC CTCAGAGCCA GTGTGGTGGG GGCTCCCAGC
    AGTGAGGACA CAAGTCAGGG GTCTGAAGGC AAGAAGCAGG AGGCCTCAGC CACTCAGGTG
    ACCAAGAGGA ACCTCCCACT GACGCAGGCT GCCCTGAAGG TTCTTGCCCA GAAAGCCAGT
    GAGGCCCAGC CTCCTGCTGC CAAGACCCCG TCTTCAAGTG GGACTGACAG TGCTGTGGGA
    ACTCTCTCCA CGGTGAGTCC CCAGAGCACC CCTACCCAGG TCAGAATGGC CAACAAGCTC
    AGAAAGCCTG AGGTTCCTGC AGCTCAGCGG GCCACAGCTA CTCCCTCGGG GCACCCCACA
    CCCAAGGCTC CTGCAACCTC AGATGACAGC AATAGCAGCT CTTCGGGAAG CGAGGACGAA
    GCCAAGGGGT CCCAGGCGGC CCCGTCAGCC CACAGGCCAG GCTCAGCCCC CTCCAGGAGG
    GAGACCTTGG TGGAAGAGAC CACAACAGAG TCCAGTGACG ATGAGGTGGT GGCACCTTCC
    CAGTCTCTCC TCTCAGGTTT TGTGACTCCT GGGCTGACTC CAGCTGGTTC CAAGACTTCA
    AAGGCCACTC CCAAACCAGA CACCAGCCCC TCTGTTTCCT CTACTCCGGC CACCAGAGAT
    GCCGCAGAAG GCAATCAGGA AGTGGAGCCC CAGCAAGCAG CAGGCACCAT GTCCCCTAAA
    ACAGGCAGAA AAGAGGCTGG TGCCACACCT CAGAAGCCCG GGAAGGGGCC TGGGAGTACC
    CCGGCCTCCA TGCTGGCGCT GCAGAGCAAC ATCACCCAGC GCCTCCTGAG CGAGCCCTGG
    CCCCTGAGTG AGGCGCAGGT GCAGGCCTCG GTGGCAAAAG TGCTCACGGA GCTGCTGGAG
    CAGGAGAGGA AGAAGGCCGT GGAGACCGCC AAGGAAGGCA ACCGGAAGGG CCGCCTGGGC
    CATAAGCGGA AGCTGTCCGA AGACCAGACA GCCCCCAAGG CCCCCAAGAG CAAGAAAAAG
    AAGCAGTTGG CAGCTGCAGA TAGTGGGGAG CATGTGGTCT CCTCAGAAAA GGCGCCCAGG
    ACTGTCAAAG GGAAATCCAA GAAGGACAGA GCAAGTGGTG ACGCCAAGGA GAAGAAGGAG
    AAGGAGTCTC CCGGCTCTCA GGGGGCCAAG GAGAAGCCAG TAGGGGAGCT GGGGACTCCG
    AAGAGTGACG GGGGAGACCA CGGCAACCTT AAGATCAAGA AAGAGAAGAA GAAATCTGAC
    AAGAAAAAAA AAGACAAGGA GAAAAAGGAA AAGAAGAAGA AAACAAAAAA GGCCTCCACC
    AAAGACCCTG GCTCACCATC ACAGAAGAAA AAGAAGAGAA AGAAGAAGAC GGCAGAGCAG
    ACTGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_027826206.1
    CDS50..3958
    Translation

    Target ORF information:

    RefSeq Version XM_027970405.1
    Organism Ovis aries(sheep)
    Definition Ovis aries treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_027970405.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGCTGAGG CCAGGAAGCG GCGGGAGCTC CTTCCCCTGA TCTACCAGCA TCTGCTGCAG 
    GCGGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA AATTTTCCTG
    AGTCAGCCTG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACTTC GGAGATTGGC
    CGGAAACGGA AGGCAGAGGA AGATGCGGCC TTGCAGGCCA AGAGGACCCG CGTGTCAGAC
    CCCATCAGCA GCTCAGAGAG CTCGGAAGAG GAGGAGGAGG AGGAGGAGGC AGAAGCCAAA
    ACCACCAAAC CCACCCCGAG ATTAGCAGCT ACCAACTCCT CAGTCACAGG GACAGTTTCG
    TCTTCAAGTG TGAAAGAAAA AGCCAAGGCA AAGACCACGA AAGCCAGCAA GACGGTGAAC
    TCCACGACAC ACCCTGCCCC GGGAAAGGCA GTGGCCCACC TCCTCACTGG GAAGTCACCC
    CAGAAGACGA CGGGGCCCTC GGCAAATGCT GTCTTGGTGT CAGAAACTGA GGAGGAGGGC
    AGCGTGTCAG CTCTCAGAAC CGCTGCCACG CCTGGAATGG CATCTGCCGA CCAGGCTGAT
    AGCTCCAGTG AGGATACCAC CAGCTCGAGC GATGAGACGG ACTTGGAGGT GAAAGCCGCA
    GTAAAACCAC TGCAGGTCAG AGCCTCAGCC ACTGCAGTCA AGGAGTCTCC GAGAAAAAGT
    GCAGCCCCAA CTCCAGGGAA GGCAGGGGTT GTGACACCCC AAGTCAAAGG CGGTGCTGTA
    ACCCCAGCCA GCAGGGCCAA GAAGCCAGAA GAGGTCTCAG AGAGCAGCGA GGAGTCCGAT
    GAGAGTGAGG AGGAGGCCCC GGTGAAGGCC TTGGAGAAAA CCACCCCAAT CAGAGTTGCT
    CCTAGTCCTG CCAAGGGGAC CCCTGGGAAA GGGGCCACCC CAGCAGCCCC TGGGAAGGCA
    GGGCCCTTAG GGAAGCCAGA GGAGGACTCG GAGAGCAGCA GCGAGGAGTC AGACAGCGAG
    GAGGAGACAC AAGCTGTCAA GACCCCAGTG CAGGCAAAGC CCTCTGGGAA GATCCCCCAG
    GTTAAAGCTG CCTCTGCAGC ACCCACCCAG GTGCTTTCCC CTAAGAAAGG GGCCCCTCCA
    GCACCCGCTG GCAAGGCAGG GCCTGCAGCC ACCCAAGCCC AGCCCAGGAA GCAGGAGAAG
    GAGGAGAGCA GCAGCAGCAG CAGCGAGGAA TCAGACAGCG AGGGCGAGGC GCCCACGGCC
    ACGCGGCAGA CTACAAGCCT GGCTCAGGCA AAGCCCTTGG AGAAAAACTC TCAGGTCAGA
    GCTGCCTCAG CCACGGGTGC AGGACCCTCG GCGAAGGGCA CAGTCCCTGC GCCCCCTCAG
    AAGGCAAGGT CTGTGGCCGC CCAGGACGGG AAGCAGGAGG ACTCGGAGAG CAGCAGTGAG
    GAGGAGTCGG ACAGCGAGGA GGAGATGCAG AGGGCAGGGA CCTCAGCCCA GGCACAGTCC
    TCGGGGAAAG CCCTCCAGGT CAGACCTTCC TCAGGTCCCA CCAAGGGGCC CCCTCAGAAG
    GCCGGACCTG CAACCACCCA GGTCAAGGTC GAAAGGGCCG AGGAAGACTC GGAGAGCAGT
    GAGGAGGAAT CAGACAGTGA GGACGAAGCA CCTGTAGCCA AGACTCCAGC CCAGGTGAAG
    ACTTCAGTGA AAACCCCTCA GACCAAAGCC AACCCTTCTG CCACCAGAGT GGCTTCAGCC
    AAAGCAGCAG CACCAGCTCC TGGAAAGGGG GGCCTTGCTG TTGCTCAAGC CAAAGTGGGG
    TCCCCAGCCA AGGTAACCCC AGCAGCGAGA GCCCCCCAGA GCAGCGCCGT CTCTGGGAGC
    GGCCAGGTGT CCATGCCGGC TGTGGGGAAG GCAGCGGCTG CAGCAGCCCA GGCCCAGCCG
    GGGCCTGTCG GGGGCTCACA GGAGGACTCG GAGAGCAGTG AGGAGGAGTC GGACAGTGAG
    GGAGAGGCAC CTCCTCAGGC GAAGCCCTCA GGAAAGACCC CCCAGGTCAA AACTGCCTCA
    GCCCCCACCA AGGCGTGCCC CAAGAAAGGG GCTGCCCCAG TACCCCCTGG GAAGGCAGGA
    CCCCCAGCCG CCCAGGCCAG GAGGCAGGAG GAGGACTCGG AGAGCAGCAG CGAGGAGGAG
    TCAGACAGTA ACGGGGACGT GCCAGCAGCT GCACCTCCAG CCCAGAAGGA GGGTAGCTCC
    AAAACTGCCA CAAGCAAGAC CCTGACTCCA GCATCCCTGG AGAAGAAAGC AGAGGAGTCC
    TCAACCAGCA GTGATGAGGA CCTGCCGTCG AACCAGGTGA TTAAAACCCC TCTGATTTTT
    GTCGACCCTA ATCGTAGTCC AGCTAGCCCA GCTGCTACCC CCACCCAAGC GCAGGCTACA
    GGCACCCCAA GGAAGGCTCA GGCCTTGGAG AGCACAGCCA GGAGCTCCTC TGAGAGCGAG
    GACGAGGACG TGATCCCCGC TACACAGTGC CCGACTCCTG CCACTAGGAC CAGCGTGGTG
    ACTGTGCCCA CAGCACCTCC AAAGGCGGCC CTCAGAGCCA GTGTGGTGGG GGCTCCCAGC
    AGTGAGGACA CAAGTCAGGG GTCTGAAGGC AAGAAGCAGG AGGCCTCAGC CACTCAGGTG
    ACCAAGAGGA ACCTCCCACT GACGCAGGCT GCCCTGAAGG TTCTTGCCCA GAAAGCCAGT
    GAGGCCCAGC CTCCTGCTGC CAAGACCCCG TCTTCAAGTG GGACTGACAG TGCTGTGGGA
    ACTCTCTCCA CGGTGAGTCC CCAGAGCACC CCTACCCAGG TCAGAATGGC CAACAAGCTC
    AGAAAGCCTG AGGTTCCTGC AGCTCAGCGG GCCACAGCTA CTCCCTCGGG GCACCCCACA
    CCCAAGGCTC CTGCAACCTC AGATGACAGC AATAGCAGCT CTTCGGGAAG CGAGGACGAA
    GCCAAGGGGT CCCAGGCGGC CCCGTCAGCC CACAGGCCAG GCTCAGCCCC CTCCAGGAGG
    GAGACCTTGG TGGAAGAGAC CACAACAGAG TCCAGTGACG ATGAGGTGGT GGCACCTTCC
    CAGTCTCTCC TCTCAGGTTT TGTGACTCCT GGGCTGACTC CAGCTGGTTC CAAGACTTCA
    AAGGCCACTC CCAAACCAGA CACCAGCCCC TCTGTTTCCT CTACTCCGGC CACCAGAGAT
    GCCGCAGAAG GCAATCAGGA AGTGGAGCCC CAGCAAGCAG CAGGCACCAT GTCCCCTAAA
    ACAGGCAGAA AAGAGGCTGG TGCCACACCT CAGAAGCCCG GGAAGGGGCC TGGGAGTACC
    CCGGCCTCCA TGCTGGCGCT GCAGAGCAAC ATCACCCAGC GCCTCCTGAG CGAGCCCTGG
    CCCCTGAGTG AGGCGCAGGT GCAGGCCTCG GTGGCAAAAG TGCTCACGGA GCTGCTGGAG
    CAGGAGAGGA AGAAGGCCGT GGAGACCGCC AAGGAAGGCA ACCGGAAGGG CCGCCTGGGC
    CATAAGCGGA AGCTGTCCGA AGACCAGACA GCCCCCAAGG CCCCCAAGAG CAAGAAAAAG
    AAGCAGTTGG CAGCTGCAGA TAGTGGGGAG CATGTGGTCT CCTCAGAAAA GGCGCCCAGG
    ACTGTCAAAG GGAAATCCAA GAAGGACAGA GCAAGTGGTG ACGCCAAGGA GAAGAAGGAG
    AAGGAGTCTC CCGGCTCTCA GGGGGCCAAG GAGAAGCCAG TAGGGGAGCT GGGGACTCCG
    AAGAGTGACG GGGGAGACCA CGGCAACCTT AAGATCAAGA AAGAGAAGAA GAAATCTGAC
    AAGAAAAAAA AAGACAAGGA GAAAAAGGAA AAGAAGAAGA AAACAAAAAA GGCCTCCACC
    AAAGACCCTG GCTCACCATC ACAGAAGAAA AAGAAGAGAA AGAAGAAGAC GGCAGAGCAG
    ACTGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OOv03145
    Clone ID Related Accession (Same CDS sequence) XM_012113401.2
    Accession Version XM_012113401.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4674bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-16
    Organism Ovis aries musimon(Ovis orientalis musimon)
    Product treacle protein isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942253.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012113401.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGCTGAGG CCAGGAAGCG GCGGGAGCTC CTTCCCCTGA TCTACCAGCA TCTGCTGCAG 
    GCGGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA AATTTTCCTG
    AGTCAGCCTG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACTTC GGAGATTGGC
    CGGAAACGGA AGGCAGAGGA AGATGCGGCC TTGCAGGCCA AGAGGACCCG CGTGTCAGAC
    CCCATCAGCA GCTCAGAGAG CTCGGAAGAG GAGGAGGAGG AGGAGGAGGC AGAAGCCAAA
    ACCACCAAAC CCACCCCGAG ATTAGCAGCT ACCAACTCCT CAGTCACAGG GACAGTTTCG
    TCTTCAAATG TGAAAGAAAA AGCCAAGGCA AAGACCACGA AAGCCAGCAA GACGGTGAAC
    TCCACGACAC ACCCTGCCCC GGGAAAGGCA GTGGCCCACC TCCTCACTGG GAAGTCACCC
    CAGAAGACGA CGGGGCCCTC GGCAAATGCT GTCTTGGTGT CAGAAACTGA GGAGGAGGGC
    AGCGTGTCAG CTCTCAGAAC CGCTGCCACG CCTGGAATGG CATCTGCCGA CCAGGCTGAT
    AGCTCCAGTG AGGATACCAC CAGCTCGAGC GATGAGACGG ACTTGGAGGT GAAAGCCGCA
    GTAAAACCAC TGCAGGTCAG AGCCTCAGCC ACTGCAGTCA AGGAGTCTCC GAGAAAAAGT
    GCAGCCCCAA CTCCAGGGAA GGCAGGGGTT GTGACACCCC AAGTCAAAGG CGGTGCTGTA
    ACCCCAGCCA GCAGGGCCAA GAAGCCAGAA GAGGTCTCAG AGAGCAGCGA GGAGTCCGAT
    GAGAGTGAGG AGGAGGCCCC GGTGAAGGCC TTGGAGAAAA CCACCCCAAT CAGAGTTGCT
    CCTAGTCCTG CCAAGGGGAC CCCTGGGAAA GGGGCCACCC CAGCAGCCCC TGGGAAGGCA
    GGGCCCTTAG GGAAGCCAGA GGAGGACTCG GAGAGCAGCA GCGAGGAGTC AGACAGCGAG
    GAGGAGACAC AAGCTGTCAA GACCCCAGTG CAGGTGGGAC CTGGAGAGAG GGCCCCGGAG
    CTGGCACCCA CCCCAGGAGG CCTCCTGAGG GCTGATTCTC CCACTCCACG TGCCCTGGCC
    ACGCAAGGGC CACGTGGAGT CTGTGGTGTC TTTGACCCCG TCTGCTATCC TGTTTCCTCA
    AGTCCACTGT CTGGGCTCTC TCCACTGATT CTGTTTCTCT CTCTTCAGGC AAAGCCCTCT
    GGGAAGATCC CCCAGGTTAA AGCTGCCTCT GCAGCACCCA CCCAGGTGCT TTCCCCTAAG
    AAAGGGGCCC CTCCAGCACC CGCTGGCAAG GCAGGGCCTG CAGCCACCCA AGCCCAGCCC
    AGGAAGCAGG AGAAGGAGGA GAGCAGCAGC AGCAGCAGCG AGGAATCAGA CAGCGAGGGC
    GAGGCGCCCA CGGCCACGCG GCAGACTACA AGCCTGGCTC AGGCAAAGCC CTTGGAGAAA
    AACTCTCAGG TCAGAGCTGC CTCAGCCACG GGTGCAGGAC CCTCGGCGAA GGGCACAGTC
    CCTGCGCCCC CTCAGAAGGC AAGGTCTGTG GCCGCCCAGG ACGGGAAGCA GGAGGACTCG
    GAGAGCAGCA GTGAGGAGGA GTCGGACAGC GAGGAGGAGA TGCAGAGGGC AGGGACCTCA
    GCCCAGGCAC AGTCCTCGGG GAAAGCCCTC CAGGTCAGAC CTTCCTCAGG TCCCACCAAG
    GGGCCCCCTC AGAAGGCCGG GCCTGCAACC ACCCAGGTCA AGGTCGAAAG GGCCGAGGAA
    GACTCGGAGA GCAGTGAGGA GGAATCAGAC AGTGAGGACG AAGCACCTGT AGCCAAGACT
    CCAGCCCAGG CAAAATCAAC TGTGAAAACT TCTCAGATCA AGGCCTCCCC AAGGAAGGGC
    ACCCCCATTA CACCCACATC TGCCAGGGTC CCCCCAGTGC AAGTCGGCAC ACCATCCCCC
    CGGACAGCAG CAACAGTGAG TACGCCCACC TGTGCATCAT CCCCAGCCAT AGTCAGGCAC
    ACCCAGAAGC CAGGGGAGGA CTCTTCAAGC AGCGAGGAGT CTGAGAGCGA GGAGGAGACA
    GCTCCTGCTG CAACAGGGGG ACAGATGAAG TCTGTAGGGA AGAGCCTCCC AGTGAAAGCT
    GCCTCAGCAT CCACCAAGGG GCCCTCAGGA AAAGGGACCA CCCCAGGGCC CCCTGGGAAG
    GCAGGACCCC CCGTGGCACA GGTCAAGGCT GAGGTGCAGG AAGACTCAGA GAGCAGTGAG
    GAGTCGGACA GTGAGGAAGC GGCTGCAGCT CCCGCACAGG TGAAGACTTC AGTGAAAACC
    CCTCAGACCA AAGCCAACCC TTCTGCCACC AGAGTGGCTT CAGCCAAAGC AGCAGCACCA
    GCTCCTGGAA AGGGGGGCCT TGCTGTTGCT CAAGCCAAAG TGGGGTCCCC AGCCAAGGTA
    ACCCCAGCAG CGAGAGCCCC CCAGAGCAGC GCCGTCTCTG GGAGCGGCCA GGTGTCCATG
    CCGGCTGTGG GGAAGGCAGC GGCTGCAGCA GCCCAGGCCC AGCCGGGGCC TGTCGGGGGC
    TCACAGGAGG ACTCGGAGAG CAGTGAGGAG GAGTCGGACA GTGAGGGAGA GGCACCTCCT
    CAGGCGAAGC CCTCAGGAAA GACCCCCCAG GTCAAAACTG CCTCAGCCCC CACCAAGGCG
    TGCCCCAAGA AAGGGGCTGC CCCAGTACCC CCTGGGAAGG CAGGACCCCC AGCCGCCCAG
    GCCAGGAGGC AGGAGGAGGA CTCGGAGAGC AGCAGCGAGG AGGAGTCAGA CAGTGACGGG
    GACGTGCCAG CAGCTGCACC TCCAGCCCAG AAGGAGGGTA GCTCCAAAAC TGCCACAAGC
    AAGACCCTGA CTCCAGCATC CCTGGAGAAG AAAGCAGAGG AGTCCTCAAC CAGCAGTGAT
    GAGGACCTGC CGTCGAACCA GGTGATTAAA ACCCCTCTGA TTTTTGTCGA CCCTAATCGT
    AGTCCAGCTA GCCCAGCTGC TACCCCCACC CAAGCGCAGG CTACAGGCAC CCCAAGGAAG
    GCTCAGGCCT TGGAGAGCAC AACCAGGAGC TCCTCTGAGA GCGAGGACGA GGACGTGATC
    CCCGCTACAC AGTGCCCGAC TCCTGCCACT AGGACCAGCG TGGTGACTGT GCCCACAGCA
    CCTCCAAAGG CGGCCCTCAG AGCCAGTGTG GTGGGGGCTC CCAGCAGTGA GGACACAAGT
    CAGGGGTCTG AAGGCAAGAA GCAGGAGGCC TCAGCCACTC AGGTGACCAA GAGGAACCTC
    CCACTGACGC AGGCTGCCCT GAAGGTTCTT GCCCAGAAAG CCAGTGAGGC CCAGCCTCCT
    GCTGCCAAGA CCCCGTCTTC AAGTGGGACT GACAGTGCTG TGGGAACTCT CTCCACGGTG
    AGTCCCCAGA GCACCCCTAC CCAGGTCAGA ATGGCCAACA AGCTCAGAAA GCCTGAGGTT
    CCTGCAGCTC AGCGGGCCAC AGCTACTCCC TCGGGGCACC CCACACCCAA GGCTCCTGCA
    ACCTCAGATG ACAGCAATAG CAGCTCTTCG GGAAGCGAGG ACGAAGCCAA GGGGTCCCAG
    GCGGCCCCGT CAGCCCACAG GCCAGGCTCA GCCCCCTCCA GGAGGGAGAC CTTGGTGGAA
    GAGACCACAA CAGAGTCCAG TGACGATGAG GTGGTGGCAC CTTCCCAGTC TCTCCTCTCA
    GGTTTTGTGA CTCCTGGGCT GACTCCAGCT GGTTCCAAGA CTTCAAAGGC CACTCCCAAA
    CCAGACACCA GCCCCTCTGT TTCCTCTACT CCGGCCACCA GAGATGCCGC AGAAGGCAAT
    CAGGAAGTGG AGCCCCAGCA AGCAGCAGGC ACCATGTCCC CTAAAACAGG CAGAAAAGAG
    GCTGGTGCCA CACCTCAGAA GCCCGGGAAG GGGCCTGGGA GTACCCCGGC CTCCATGCTG
    GCGCTGCAGA GCAACATCAC CCAGCGCCTC CTGAGCGAGC CCTGGCCCCT GAGTGAGGCG
    CAGGTGCAGG CCTCGGTGGC AAAAGTGCTC ACGGAGCTGC TGGAGCAGGA GAGGAAGAAG
    GCCGTGGAGA CCGCCAAGGA AGGCAACCGG AAGGGCCGCC TGGGCCATAA GCGGAAGCTG
    TCCGAAGACC AGACAGCCCC CAAGGCCCCC AAGAGCAAGA AAAAGAAGCA GTTGGCAGCT
    GCAGATAGTG GGGAGCATGT GGTCTCCTCA GAAAAGGCGC CCAGGACTGT CAAAGGGAAA
    TCCAAGAAGG ACAGAGCAAG TGGTGACGCC AAGGAGAAGA AGGAGAAGGA GTCTCCCGGC
    TCTCAGGGGG CCAAGGAGAA GCCAGTAGGG GAGCTGGGGA CTCCGAAGAG TGACGGGGGA
    GACCACGGCA ACCTTAAGAT CAAGAAAGAG AAGAAGAAAT CTGACAAGAG ATGGGCAAAG
    CCAGAACCAG GTCCTGGAGC AGCGGAGCCA CCACCACCAG GACGGCGCTC ACAGCTCCAG
    GGCATGTTCC GCTCGAAGAG CCCTGCCCTC CCCCACGGCG GCCACAGCCA CGTGCACTGG
    CAGCCGAGGC TCCTGGGGTC CAGGCTCCTG GATAGCAGCT GCCCCTTGCT GCATTCTGTG
    CTGGAGTCCT CGTGCAGCCG GGCCACGTTT AACCCCCACC CCAGCCCTGT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011968791.1
    CDS48..4721
    Translation

    Target ORF information:

    RefSeq Version XM_012113401.2
    Organism Ovis aries musimon(Ovis orientalis musimon)
    Definition Ovis aries musimon Treacher Collins-Franceschetti syndrome 1 (TCOF1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012113401.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGCTGAGG CCAGGAAGCG GCGGGAGCTC CTTCCCCTGA TCTACCAGCA TCTGCTGCAG 
    GCGGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA AATTTTCCTG
    AGTCAGCCTG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACTTC GGAGATTGGC
    CGGAAACGGA AGGCAGAGGA AGATGCGGCC TTGCAGGCCA AGAGGACCCG CGTGTCAGAC
    CCCATCAGCA GCTCAGAGAG CTCGGAAGAG GAGGAGGAGG AGGAGGAGGC AGAAGCCAAA
    ACCACCAAAC CCACCCCGAG ATTAGCAGCT ACCAACTCCT CAGTCACAGG GACAGTTTCG
    TCTTCAAATG TGAAAGAAAA AGCCAAGGCA AAGACCACGA AAGCCAGCAA GACGGTGAAC
    TCCACGACAC ACCCTGCCCC GGGAAAGGCA GTGGCCCACC TCCTCACTGG GAAGTCACCC
    CAGAAGACGA CGGGGCCCTC GGCAAATGCT GTCTTGGTGT CAGAAACTGA GGAGGAGGGC
    AGCGTGTCAG CTCTCAGAAC CGCTGCCACG CCTGGAATGG CATCTGCCGA CCAGGCTGAT
    AGCTCCAGTG AGGATACCAC CAGCTCGAGC GATGAGACGG ACTTGGAGGT GAAAGCCGCA
    GTAAAACCAC TGCAGGTCAG AGCCTCAGCC ACTGCAGTCA AGGAGTCTCC GAGAAAAAGT
    GCAGCCCCAA CTCCAGGGAA GGCAGGGGTT GTGACACCCC AAGTCAAAGG CGGTGCTGTA
    ACCCCAGCCA GCAGGGCCAA GAAGCCAGAA GAGGTCTCAG AGAGCAGCGA GGAGTCCGAT
    GAGAGTGAGG AGGAGGCCCC GGTGAAGGCC TTGGAGAAAA CCACCCCAAT CAGAGTTGCT
    CCTAGTCCTG CCAAGGGGAC CCCTGGGAAA GGGGCCACCC CAGCAGCCCC TGGGAAGGCA
    GGGCCCTTAG GGAAGCCAGA GGAGGACTCG GAGAGCAGCA GCGAGGAGTC AGACAGCGAG
    GAGGAGACAC AAGCTGTCAA GACCCCAGTG CAGGTGGGAC CTGGAGAGAG GGCCCCGGAG
    CTGGCACCCA CCCCAGGAGG CCTCCTGAGG GCTGATTCTC CCACTCCACG TGCCCTGGCC
    ACGCAAGGGC CACGTGGAGT CTGTGGTGTC TTTGACCCCG TCTGCTATCC TGTTTCCTCA
    AGTCCACTGT CTGGGCTCTC TCCACTGATT CTGTTTCTCT CTCTTCAGGC AAAGCCCTCT
    GGGAAGATCC CCCAGGTTAA AGCTGCCTCT GCAGCACCCA CCCAGGTGCT TTCCCCTAAG
    AAAGGGGCCC CTCCAGCACC CGCTGGCAAG GCAGGGCCTG CAGCCACCCA AGCCCAGCCC
    AGGAAGCAGG AGAAGGAGGA GAGCAGCAGC AGCAGCAGCG AGGAATCAGA CAGCGAGGGC
    GAGGCGCCCA CGGCCACGCG GCAGACTACA AGCCTGGCTC AGGCAAAGCC CTTGGAGAAA
    AACTCTCAGG TCAGAGCTGC CTCAGCCACG GGTGCAGGAC CCTCGGCGAA GGGCACAGTC
    CCTGCGCCCC CTCAGAAGGC AAGGTCTGTG GCCGCCCAGG ACGGGAAGCA GGAGGACTCG
    GAGAGCAGCA GTGAGGAGGA GTCGGACAGC GAGGAGGAGA TGCAGAGGGC AGGGACCTCA
    GCCCAGGCAC AGTCCTCGGG GAAAGCCCTC CAGGTCAGAC CTTCCTCAGG TCCCACCAAG
    GGGCCCCCTC AGAAGGCCGG GCCTGCAACC ACCCAGGTCA AGGTCGAAAG GGCCGAGGAA
    GACTCGGAGA GCAGTGAGGA GGAATCAGAC AGTGAGGACG AAGCACCTGT AGCCAAGACT
    CCAGCCCAGG CAAAATCAAC TGTGAAAACT TCTCAGATCA AGGCCTCCCC AAGGAAGGGC
    ACCCCCATTA CACCCACATC TGCCAGGGTC CCCCCAGTGC AAGTCGGCAC ACCATCCCCC
    CGGACAGCAG CAACAGTGAG TACGCCCACC TGTGCATCAT CCCCAGCCAT AGTCAGGCAC
    ACCCAGAAGC CAGGGGAGGA CTCTTCAAGC AGCGAGGAGT CTGAGAGCGA GGAGGAGACA
    GCTCCTGCTG CAACAGGGGG ACAGATGAAG TCTGTAGGGA AGAGCCTCCC AGTGAAAGCT
    GCCTCAGCAT CCACCAAGGG GCCCTCAGGA AAAGGGACCA CCCCAGGGCC CCCTGGGAAG
    GCAGGACCCC CCGTGGCACA GGTCAAGGCT GAGGTGCAGG AAGACTCAGA GAGCAGTGAG
    GAGTCGGACA GTGAGGAAGC GGCTGCAGCT CCCGCACAGG TGAAGACTTC AGTGAAAACC
    CCTCAGACCA AAGCCAACCC TTCTGCCACC AGAGTGGCTT CAGCCAAAGC AGCAGCACCA
    GCTCCTGGAA AGGGGGGCCT TGCTGTTGCT CAAGCCAAAG TGGGGTCCCC AGCCAAGGTA
    ACCCCAGCAG CGAGAGCCCC CCAGAGCAGC GCCGTCTCTG GGAGCGGCCA GGTGTCCATG
    CCGGCTGTGG GGAAGGCAGC GGCTGCAGCA GCCCAGGCCC AGCCGGGGCC TGTCGGGGGC
    TCACAGGAGG ACTCGGAGAG CAGTGAGGAG GAGTCGGACA GTGAGGGAGA GGCACCTCCT
    CAGGCGAAGC CCTCAGGAAA GACCCCCCAG GTCAAAACTG CCTCAGCCCC CACCAAGGCG
    TGCCCCAAGA AAGGGGCTGC CCCAGTACCC CCTGGGAAGG CAGGACCCCC AGCCGCCCAG
    GCCAGGAGGC AGGAGGAGGA CTCGGAGAGC AGCAGCGAGG AGGAGTCAGA CAGTGACGGG
    GACGTGCCAG CAGCTGCACC TCCAGCCCAG AAGGAGGGTA GCTCCAAAAC TGCCACAAGC
    AAGACCCTGA CTCCAGCATC CCTGGAGAAG AAAGCAGAGG AGTCCTCAAC CAGCAGTGAT
    GAGGACCTGC CGTCGAACCA GGTGATTAAA ACCCCTCTGA TTTTTGTCGA CCCTAATCGT
    AGTCCAGCTA GCCCAGCTGC TACCCCCACC CAAGCGCAGG CTACAGGCAC CCCAAGGAAG
    GCTCAGGCCT TGGAGAGCAC AACCAGGAGC TCCTCTGAGA GCGAGGACGA GGACGTGATC
    CCCGCTACAC AGTGCCCGAC TCCTGCCACT AGGACCAGCG TGGTGACTGT GCCCACAGCA
    CCTCCAAAGG CGGCCCTCAG AGCCAGTGTG GTGGGGGCTC CCAGCAGTGA GGACACAAGT
    CAGGGGTCTG AAGGCAAGAA GCAGGAGGCC TCAGCCACTC AGGTGACCAA GAGGAACCTC
    CCACTGACGC AGGCTGCCCT GAAGGTTCTT GCCCAGAAAG CCAGTGAGGC CCAGCCTCCT
    GCTGCCAAGA CCCCGTCTTC AAGTGGGACT GACAGTGCTG TGGGAACTCT CTCCACGGTG
    AGTCCCCAGA GCACCCCTAC CCAGGTCAGA ATGGCCAACA AGCTCAGAAA GCCTGAGGTT
    CCTGCAGCTC AGCGGGCCAC AGCTACTCCC TCGGGGCACC CCACACCCAA GGCTCCTGCA
    ACCTCAGATG ACAGCAATAG CAGCTCTTCG GGAAGCGAGG ACGAAGCCAA GGGGTCCCAG
    GCGGCCCCGT CAGCCCACAG GCCAGGCTCA GCCCCCTCCA GGAGGGAGAC CTTGGTGGAA
    GAGACCACAA CAGAGTCCAG TGACGATGAG GTGGTGGCAC CTTCCCAGTC TCTCCTCTCA
    GGTTTTGTGA CTCCTGGGCT GACTCCAGCT GGTTCCAAGA CTTCAAAGGC CACTCCCAAA
    CCAGACACCA GCCCCTCTGT TTCCTCTACT CCGGCCACCA GAGATGCCGC AGAAGGCAAT
    CAGGAAGTGG AGCCCCAGCA AGCAGCAGGC ACCATGTCCC CTAAAACAGG CAGAAAAGAG
    GCTGGTGCCA CACCTCAGAA GCCCGGGAAG GGGCCTGGGA GTACCCCGGC CTCCATGCTG
    GCGCTGCAGA GCAACATCAC CCAGCGCCTC CTGAGCGAGC CCTGGCCCCT GAGTGAGGCG
    CAGGTGCAGG CCTCGGTGGC AAAAGTGCTC ACGGAGCTGC TGGAGCAGGA GAGGAAGAAG
    GCCGTGGAGA CCGCCAAGGA AGGCAACCGG AAGGGCCGCC TGGGCCATAA GCGGAAGCTG
    TCCGAAGACC AGACAGCCCC CAAGGCCCCC AAGAGCAAGA AAAAGAAGCA GTTGGCAGCT
    GCAGATAGTG GGGAGCATGT GGTCTCCTCA GAAAAGGCGC CCAGGACTGT CAAAGGGAAA
    TCCAAGAAGG ACAGAGCAAG TGGTGACGCC AAGGAGAAGA AGGAGAAGGA GTCTCCCGGC
    TCTCAGGGGG CCAAGGAGAA GCCAGTAGGG GAGCTGGGGA CTCCGAAGAG TGACGGGGGA
    GACCACGGCA ACCTTAAGAT CAAGAAAGAG AAGAAGAAAT CTGACAAGAG ATGGGCAAAG
    CCAGAACCAG GTCCTGGAGC AGCGGAGCCA CCACCACCAG GACGGCGCTC ACAGCTCCAG
    GGCATGTTCC GCTCGAAGAG CCCTGCCCTC CCCCACGGCG GCCACAGCCA CGTGCACTGG
    CAGCCGAGGC TCCTGGGGTC CAGGCTCCTG GATAGCAGCT GCCCCTTGCT GCATTCTGTG
    CTGGAGTCCT CGTGCAGCCG GGCCACGTTT AACCCCCACC CCAGCCCTGT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OOv03146
    Clone ID Related Accession (Same CDS sequence) XM_012113402.2 , XM_012113403.2
    Accession Version XM_012113402.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4554bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-16
    Organism Ovis aries musimon(Ovis orientalis musimon)
    Product treacle protein isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942253.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012113402.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGCTGAGG CCAGGAAGCG GCGGGAGCTC CTTCCCCTGA TCTACCAGCA TCTGCTGCAG 
    GCGGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA AATTTTCCTG
    AGTCAGCCTG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACTTC GGAGATTGGC
    CGGAAACGGA AGGCAGAGGA AGATGCGGCC TTGCAGGCCA AGAGGACCCG CGTGTCAGAC
    CCCATCAGCA GCTCAGAGAG CTCGGAAGAG GAGGAGGAGG AGGAGGAGGC AGAAGCCAAA
    ACCACCAAAC CCACCCCGAG ATTAGCAGCT ACCAACTCCT CAGTCACAGG GACAGTTTCG
    TCTTCAAATG TGAAAGAAAA AGCCAAGGCA AAGACCACGA AAGCCAGCAA GACGGTGAAC
    TCCACGACAC ACCCTGCCCC GGGAAAGGCA GTGGCCCACC TCCTCACTGG GAAGTCACCC
    CAGAAGACGA CGGGGCCCTC GGCAAATGCT GTCTTGGTGT CAGAAACTGA GGAGGAGGGC
    AGCGTGTCAG CTCTCAGAAC CGCTGCCACG CCTGGAATGG CATCTGCCGA CCAGGCTGAT
    AGCTCCAGTG AGGATACCAC CAGCTCGAGC GATGAGACGG ACTTGGAGGT GAAAGCCGCA
    GTAAAACCAC TGCAGGTCAG AGCCTCAGCC ACTGCAGTCA AGGAGTCTCC GAGAAAAAGT
    GCAGCCCCAA CTCCAGGGAA GGCAGGGGTT GTGACACCCC AAGTCAAAGG CGGTGCTGTA
    ACCCCAGCCA GCAGGGCCAA GAAGCCAGAA GAGGTCTCAG AGAGCAGCGA GGAGTCCGAT
    GAGAGTGAGG AGGAGGCCCC GGTGAAGGCC TTGGAGAAAA CCACCCCAAT CAGAGTTGCT
    CCTAGTCCTG CCAAGGGGAC CCCTGGGAAA GGGGCCACCC CAGCAGCCCC TGGGAAGGCA
    GGGCCCTTAG GGAAGCCAGA GGAGGACTCG GAGAGCAGCA GCGAGGAGTC AGACAGCGAG
    GAGGAGACAC AAGCTGTCAA GACCCCAGTG CAGGTGGGAC CTGGAGAGAG GGCCCCGGAG
    CTGGCACCCA CCCCAGGAGG CCTCCTGAGG GCTGATTCTC CCACTCCACG TGCCCTGGCC
    ACGCAAGGGC CACGTGGAGT CTGTGGTGTC TTTGACCCCG TCTGCTATCC TGTTTCCTCA
    AGTCCACTGT CTGGGCTCTC TCCACTGATT CTGTTTCTCT CTCTTCAGGC AAAGCCCTCT
    GGGAAGATCC CCCAGGTTAA AGCTGCCTCT GCAGCACCCA CCCAGGTGCT TTCCCCTAAG
    AAAGGGGCCC CTCCAGCACC CGCTGGCAAG GCAGGGCCTG CAGCCACCCA AGCCCAGCCC
    AGGAAGCAGG AGAAGGAGGA GAGCAGCAGC AGCAGCAGCG AGGAATCAGA CAGCGAGGGC
    GAGGCGCCCA CGGCCACGCG GCAGACTACA AGCCTGGCTC AGGCAAAGCC CTTGGAGAAA
    AACTCTCAGG TCAGAGCTGC CTCAGCCACG GGTGCAGGAC CCTCGGCGAA GGGCACAGTC
    CCTGCGCCCC CTCAGAAGGC AAGGTCTGTG GCCGCCCAGG ACGGGAAGCA GGAGGACTCG
    GAGAGCAGCA GTGAGGAGGA GTCGGACAGC GAGGAGGAGA TGCAGAGGGC AGGGACCTCA
    GCCCAGGCAC AGTCCTCGGG GAAAGCCCTC CAGGTCAGAC CTTCCTCAGG TCCCACCAAG
    GGGCCCCCTC AGAAGGCCGG GCCTGCAACC ACCCAGGTCA AGGTCGAAAG GGCCGAGGAA
    GACTCGGAGA GCAGTGAGGA GGAATCAGAC AGTGAGGACG AAGCACCTGT AGCCAAGACT
    CCAGCCCAGG CAAAATCAAC TGTGAAAACT TCTCAGATCA AGGCCTCCCC AAGGAAGGGC
    ACCCCCATTA CACCCACATC TGCCAGGGTC CCCCCAGTGC AAGTCGGCAC ACCATCCCCC
    CGGACAGCAG CAACAGTGAG TACGCCCACC TGTGCATCAT CCCCAGCCAT AGTCAGGCAC
    ACCCAGAAGC CAGGGGAGGA CTCTTCAAGC AGCGAGGAGT CTGAGAGCGA GGAGGAGACA
    GCTCCTGCTG CAACAGGGGG ACAGATGAAG TCTGTAGGGA AGAGCCTCCC AGTGAAAGCT
    GCCTCAGCAT CCACCAAGGG GCCCTCAGGA AAAGGGACCA CCCCAGGGCC CCCTGGGAAG
    GCAGGACCCC CCGTGGCACA GGTCAAGGCT GAGGTGCAGG AAGACTCAGA GAGCAGTGAG
    GAGTCGGACA GTGAGGAAGC GGCTGCAGCT CCCGCACAGG TGAAGACTTC AGTGAAAACC
    CCTCAGACCA AAGCCAACCC TTCTGCCACC AGAGTGGCTT CAGCCAAAGC AGCAGCACCA
    GCTCCTGGAA AGGGGGGCCT TGCTGTTGCT CAAGCCAAAG TGGGGTCCCC AGCCAAGGTA
    ACCCCAGCAG CGAGAGCCCC CCAGAGCAGC GCCGTCTCTG GGAGCGGCCA GGTGTCCATG
    CCGGCTGTGG GGAAGGCAGC GGCTGCAGCA GCCCAGGCCC AGCCGGGGCC TGTCGGGGGC
    TCACAGGAGG ACTCGGAGAG CAGTGAGGAG GAGTCGGACA GTGAGGGAGA GGCACCTCCT
    CAGGCGAAGC CCTCAGGAAA GACCCCCCAG GTCAAAACTG CCTCAGCCCC CACCAAGGCG
    TGCCCCAAGA AAGGGGCTGC CCCAGTACCC CCTGGGAAGG CAGGACCCCC AGCCGCCCAG
    GCCAGGAGGC AGGAGGAGGA CTCGGAGAGC AGCAGCGAGG AGGAGTCAGA CAGTGACGGG
    GACGTGCCAG CAGCTGCACC TCCAGCCCAG AAGGAGGGTA GCTCCAAAAC TGCCACAAGC
    AAGACCCTGA CTCCAGCATC CCTGGAGAAG AAAGCAGAGG AGTCCTCAAC CAGCAGTGAT
    GAGGACCTGC CGTCGAACCA GGTGATTAAA ACCCCTCTGA TTTTTGTCGA CCCTAATCGT
    AGTCCAGCTA GCCCAGCTGC TACCCCCACC CAAGCGCAGG CTACAGGCAC CCCAAGGAAG
    GCTCAGGCCT TGGAGAGCAC AACCAGGAGC TCCTCTGAGA GCGAGGACGA GGACGTGATC
    CCCGCTACAC AGTGCCCGAC TCCTGCCACT AGGACCAGCG TGGTGACTGT GCCCACAGCA
    CCTCCAAAGG CGGCCCTCAG AGCCAGTGTG GTGGGGGCTC CCAGCAGTGA GGACACAAGT
    CAGGGGTCTG AAGGCAAGAA GCAGGAGGCC TCAGCCACTC AGGTGACCAA GAGGAACCTC
    CCACTGACGC AGGCTGCCCT GAAGGTTCTT GCCCAGAAAG CCAGTGAGGC CCAGCCTCCT
    GCTGCCAAGA CCCCGTCTTC AAGTGGGACT GACAGTGCTG TGGGAACTCT CTCCACGGTG
    AGTCCCCAGA GCACCCCTAC CCAGGTCAGA ATGGCCAACA AGCTCAGAAA GCCTGAGGTT
    CCTGCAGCTC AGCGGGCCAC AGCTACTCCC TCGGGGCACC CCACACCCAA GGCTCCTGCA
    ACCTCAGATG ACAGCAATAG CAGCTCTTCG GGAAGCGAGG ACGAAGCCAA GGGGTCCCAG
    GCGGCCCCGT CAGCCCACAG GCCAGGCTCA GCCCCCTCCA GGAGGGAGAC CTTGGTGGAA
    GAGACCACAA CAGAGTCCAG TGACGATGAG GTGGTGGCAC CTTCCCAGTC TCTCCTCTCA
    GGTTTTGTGA CTCCTGGGCT GACTCCAGCT GGTTCCAAGA CTTCAAAGGC CACTCCCAAA
    CCAGACACCA GCCCCTCTGT TTCCTCTACT CCGGCCACCA GAGATGCCGC AGAAGGCAAT
    CAGGAAGTGG AGCCCCAGCA AGCAGCAGGC ACCATGTCCC CTAAAACAGG CAGAAAAGAG
    GCTGGTGCCA CACCTCAGAA GCCCGGGAAG GGGCCTGGGA GTACCCCGGC CTCCATGCTG
    GCGCTGCAGA GCAACATCAC CCAGCGCCTC CTGAGCGAGC CCTGGCCCCT GAGTGAGGCG
    CAGGTGCAGG CCTCGGTGGC AAAAGTGCTC ACGGAGCTGC TGGAGCAGGA GAGGAAGAAG
    GCCGTGGAGA CCGCCAAGGA AGGCAACCGG AAGGGCCGCC TGGGCCATAA GCGGAAGCTG
    TCCGAAGACC AGACAGCCCC CAAGGCCCCC AAGAGCAAGA AAAAGAAGCA GTTGGCAGCT
    GCAGATAGTG GGGAGCATGT GGTCTCCTCA GAAAAGGCGC CCAGGACTGT CAAAGGGAAA
    TCCAAGAAGG ACAGAGCAAG TGGTGACGCC AAGGAGAAGA AGGAGAAGGA GTCTCCCGGC
    TCTCAGGGGG CCAAGGAGAA GCCAGTAGGG GAGCTGGGGA CTCCGAAGAG TGACGGGGGA
    GACCACGGCA ACCTTAAGAT CAAGAAAGAG AAGAAGAAAT CTGACAAGAA AAAAAAAGAC
    AAGGAGAAAA AGGAAAAGAA GAAGAAAACA AAAAAGGCCT CCACCAAAGA CCCTGGCTCA
    CCATCACAGA AGAAAAAGAA GAGAAAGAAG AAGACAGCAG AGCAGACTGT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011968792.1
    CDS48..4601
    Translation

    Target ORF information:

    RefSeq Version XM_012113402.2
    Organism Ovis aries musimon(Ovis orientalis musimon)
    Definition Ovis aries musimon Treacher Collins-Franceschetti syndrome 1 (TCOF1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012113402.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGCTGAGG CCAGGAAGCG GCGGGAGCTC CTTCCCCTGA TCTACCAGCA TCTGCTGCAG 
    GCGGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA AATTTTCCTG
    AGTCAGCCTG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACTTC GGAGATTGGC
    CGGAAACGGA AGGCAGAGGA AGATGCGGCC TTGCAGGCCA AGAGGACCCG CGTGTCAGAC
    CCCATCAGCA GCTCAGAGAG CTCGGAAGAG GAGGAGGAGG AGGAGGAGGC AGAAGCCAAA
    ACCACCAAAC CCACCCCGAG ATTAGCAGCT ACCAACTCCT CAGTCACAGG GACAGTTTCG
    TCTTCAAATG TGAAAGAAAA AGCCAAGGCA AAGACCACGA AAGCCAGCAA GACGGTGAAC
    TCCACGACAC ACCCTGCCCC GGGAAAGGCA GTGGCCCACC TCCTCACTGG GAAGTCACCC
    CAGAAGACGA CGGGGCCCTC GGCAAATGCT GTCTTGGTGT CAGAAACTGA GGAGGAGGGC
    AGCGTGTCAG CTCTCAGAAC CGCTGCCACG CCTGGAATGG CATCTGCCGA CCAGGCTGAT
    AGCTCCAGTG AGGATACCAC CAGCTCGAGC GATGAGACGG ACTTGGAGGT GAAAGCCGCA
    GTAAAACCAC TGCAGGTCAG AGCCTCAGCC ACTGCAGTCA AGGAGTCTCC GAGAAAAAGT
    GCAGCCCCAA CTCCAGGGAA GGCAGGGGTT GTGACACCCC AAGTCAAAGG CGGTGCTGTA
    ACCCCAGCCA GCAGGGCCAA GAAGCCAGAA GAGGTCTCAG AGAGCAGCGA GGAGTCCGAT
    GAGAGTGAGG AGGAGGCCCC GGTGAAGGCC TTGGAGAAAA CCACCCCAAT CAGAGTTGCT
    CCTAGTCCTG CCAAGGGGAC CCCTGGGAAA GGGGCCACCC CAGCAGCCCC TGGGAAGGCA
    GGGCCCTTAG GGAAGCCAGA GGAGGACTCG GAGAGCAGCA GCGAGGAGTC AGACAGCGAG
    GAGGAGACAC AAGCTGTCAA GACCCCAGTG CAGGTGGGAC CTGGAGAGAG GGCCCCGGAG
    CTGGCACCCA CCCCAGGAGG CCTCCTGAGG GCTGATTCTC CCACTCCACG TGCCCTGGCC
    ACGCAAGGGC CACGTGGAGT CTGTGGTGTC TTTGACCCCG TCTGCTATCC TGTTTCCTCA
    AGTCCACTGT CTGGGCTCTC TCCACTGATT CTGTTTCTCT CTCTTCAGGC AAAGCCCTCT
    GGGAAGATCC CCCAGGTTAA AGCTGCCTCT GCAGCACCCA CCCAGGTGCT TTCCCCTAAG
    AAAGGGGCCC CTCCAGCACC CGCTGGCAAG GCAGGGCCTG CAGCCACCCA AGCCCAGCCC
    AGGAAGCAGG AGAAGGAGGA GAGCAGCAGC AGCAGCAGCG AGGAATCAGA CAGCGAGGGC
    GAGGCGCCCA CGGCCACGCG GCAGACTACA AGCCTGGCTC AGGCAAAGCC CTTGGAGAAA
    AACTCTCAGG TCAGAGCTGC CTCAGCCACG GGTGCAGGAC CCTCGGCGAA GGGCACAGTC
    CCTGCGCCCC CTCAGAAGGC AAGGTCTGTG GCCGCCCAGG ACGGGAAGCA GGAGGACTCG
    GAGAGCAGCA GTGAGGAGGA GTCGGACAGC GAGGAGGAGA TGCAGAGGGC AGGGACCTCA
    GCCCAGGCAC AGTCCTCGGG GAAAGCCCTC CAGGTCAGAC CTTCCTCAGG TCCCACCAAG
    GGGCCCCCTC AGAAGGCCGG GCCTGCAACC ACCCAGGTCA AGGTCGAAAG GGCCGAGGAA
    GACTCGGAGA GCAGTGAGGA GGAATCAGAC AGTGAGGACG AAGCACCTGT AGCCAAGACT
    CCAGCCCAGG CAAAATCAAC TGTGAAAACT TCTCAGATCA AGGCCTCCCC AAGGAAGGGC
    ACCCCCATTA CACCCACATC TGCCAGGGTC CCCCCAGTGC AAGTCGGCAC ACCATCCCCC
    CGGACAGCAG CAACAGTGAG TACGCCCACC TGTGCATCAT CCCCAGCCAT AGTCAGGCAC
    ACCCAGAAGC CAGGGGAGGA CTCTTCAAGC AGCGAGGAGT CTGAGAGCGA GGAGGAGACA
    GCTCCTGCTG CAACAGGGGG ACAGATGAAG TCTGTAGGGA AGAGCCTCCC AGTGAAAGCT
    GCCTCAGCAT CCACCAAGGG GCCCTCAGGA AAAGGGACCA CCCCAGGGCC CCCTGGGAAG
    GCAGGACCCC CCGTGGCACA GGTCAAGGCT GAGGTGCAGG AAGACTCAGA GAGCAGTGAG
    GAGTCGGACA GTGAGGAAGC GGCTGCAGCT CCCGCACAGG TGAAGACTTC AGTGAAAACC
    CCTCAGACCA AAGCCAACCC TTCTGCCACC AGAGTGGCTT CAGCCAAAGC AGCAGCACCA
    GCTCCTGGAA AGGGGGGCCT TGCTGTTGCT CAAGCCAAAG TGGGGTCCCC AGCCAAGGTA
    ACCCCAGCAG CGAGAGCCCC CCAGAGCAGC GCCGTCTCTG GGAGCGGCCA GGTGTCCATG
    CCGGCTGTGG GGAAGGCAGC GGCTGCAGCA GCCCAGGCCC AGCCGGGGCC TGTCGGGGGC
    TCACAGGAGG ACTCGGAGAG CAGTGAGGAG GAGTCGGACA GTGAGGGAGA GGCACCTCCT
    CAGGCGAAGC CCTCAGGAAA GACCCCCCAG GTCAAAACTG CCTCAGCCCC CACCAAGGCG
    TGCCCCAAGA AAGGGGCTGC CCCAGTACCC CCTGGGAAGG CAGGACCCCC AGCCGCCCAG
    GCCAGGAGGC AGGAGGAGGA CTCGGAGAGC AGCAGCGAGG AGGAGTCAGA CAGTGACGGG
    GACGTGCCAG CAGCTGCACC TCCAGCCCAG AAGGAGGGTA GCTCCAAAAC TGCCACAAGC
    AAGACCCTGA CTCCAGCATC CCTGGAGAAG AAAGCAGAGG AGTCCTCAAC CAGCAGTGAT
    GAGGACCTGC CGTCGAACCA GGTGATTAAA ACCCCTCTGA TTTTTGTCGA CCCTAATCGT
    AGTCCAGCTA GCCCAGCTGC TACCCCCACC CAAGCGCAGG CTACAGGCAC CCCAAGGAAG
    GCTCAGGCCT TGGAGAGCAC AACCAGGAGC TCCTCTGAGA GCGAGGACGA GGACGTGATC
    CCCGCTACAC AGTGCCCGAC TCCTGCCACT AGGACCAGCG TGGTGACTGT GCCCACAGCA
    CCTCCAAAGG CGGCCCTCAG AGCCAGTGTG GTGGGGGCTC CCAGCAGTGA GGACACAAGT
    CAGGGGTCTG AAGGCAAGAA GCAGGAGGCC TCAGCCACTC AGGTGACCAA GAGGAACCTC
    CCACTGACGC AGGCTGCCCT GAAGGTTCTT GCCCAGAAAG CCAGTGAGGC CCAGCCTCCT
    GCTGCCAAGA CCCCGTCTTC AAGTGGGACT GACAGTGCTG TGGGAACTCT CTCCACGGTG
    AGTCCCCAGA GCACCCCTAC CCAGGTCAGA ATGGCCAACA AGCTCAGAAA GCCTGAGGTT
    CCTGCAGCTC AGCGGGCCAC AGCTACTCCC TCGGGGCACC CCACACCCAA GGCTCCTGCA
    ACCTCAGATG ACAGCAATAG CAGCTCTTCG GGAAGCGAGG ACGAAGCCAA GGGGTCCCAG
    GCGGCCCCGT CAGCCCACAG GCCAGGCTCA GCCCCCTCCA GGAGGGAGAC CTTGGTGGAA
    GAGACCACAA CAGAGTCCAG TGACGATGAG GTGGTGGCAC CTTCCCAGTC TCTCCTCTCA
    GGTTTTGTGA CTCCTGGGCT GACTCCAGCT GGTTCCAAGA CTTCAAAGGC CACTCCCAAA
    CCAGACACCA GCCCCTCTGT TTCCTCTACT CCGGCCACCA GAGATGCCGC AGAAGGCAAT
    CAGGAAGTGG AGCCCCAGCA AGCAGCAGGC ACCATGTCCC CTAAAACAGG CAGAAAAGAG
    GCTGGTGCCA CACCTCAGAA GCCCGGGAAG GGGCCTGGGA GTACCCCGGC CTCCATGCTG
    GCGCTGCAGA GCAACATCAC CCAGCGCCTC CTGAGCGAGC CCTGGCCCCT GAGTGAGGCG
    CAGGTGCAGG CCTCGGTGGC AAAAGTGCTC ACGGAGCTGC TGGAGCAGGA GAGGAAGAAG
    GCCGTGGAGA CCGCCAAGGA AGGCAACCGG AAGGGCCGCC TGGGCCATAA GCGGAAGCTG
    TCCGAAGACC AGACAGCCCC CAAGGCCCCC AAGAGCAAGA AAAAGAAGCA GTTGGCAGCT
    GCAGATAGTG GGGAGCATGT GGTCTCCTCA GAAAAGGCGC CCAGGACTGT CAAAGGGAAA
    TCCAAGAAGG ACAGAGCAAG TGGTGACGCC AAGGAGAAGA AGGAGAAGGA GTCTCCCGGC
    TCTCAGGGGG CCAAGGAGAA GCCAGTAGGG GAGCTGGGGA CTCCGAAGAG TGACGGGGGA
    GACCACGGCA ACCTTAAGAT CAAGAAAGAG AAGAAGAAAT CTGACAAGAA AAAAAAAGAC
    AAGGAGAAAA AGGAAAAGAA GAAGAAAACA AAAAAGGCCT CCACCAAAGA CCCTGGCTCA
    CCATCACAGA AGAAAAAGAA GAGAAAGAAG AAGACAGCAG AGCAGACTGT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OOv03146
    Clone ID Related Accession (Same CDS sequence) XM_012113402.2 , XM_012113403.2
    Accession Version XM_012113403.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4554bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-16
    Organism Ovis aries musimon(Ovis orientalis musimon)
    Product treacle protein isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942253.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012113403.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGCTGAGG CCAGGAAGCG GCGGGAGCTC CTTCCCCTGA TCTACCAGCA TCTGCTGCAG 
    GCGGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA AATTTTCCTG
    AGTCAGCCTG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACTTC GGAGATTGGC
    CGGAAACGGA AGGCAGAGGA AGATGCGGCC TTGCAGGCCA AGAGGACCCG CGTGTCAGAC
    CCCATCAGCA GCTCAGAGAG CTCGGAAGAG GAGGAGGAGG AGGAGGAGGC AGAAGCCAAA
    ACCACCAAAC CCACCCCGAG ATTAGCAGCT ACCAACTCCT CAGTCACAGG GACAGTTTCG
    TCTTCAAATG TGAAAGAAAA AGCCAAGGCA AAGACCACGA AAGCCAGCAA GACGGTGAAC
    TCCACGACAC ACCCTGCCCC GGGAAAGGCA GTGGCCCACC TCCTCACTGG GAAGTCACCC
    CAGAAGACGA CGGGGCCCTC GGCAAATGCT GTCTTGGTGT CAGAAACTGA GGAGGAGGGC
    AGCGTGTCAG CTCTCAGAAC CGCTGCCACG CCTGGAATGG CATCTGCCGA CCAGGCTGAT
    AGCTCCAGTG AGGATACCAC CAGCTCGAGC GATGAGACGG ACTTGGAGGT GAAAGCCGCA
    GTAAAACCAC TGCAGGTCAG AGCCTCAGCC ACTGCAGTCA AGGAGTCTCC GAGAAAAAGT
    GCAGCCCCAA CTCCAGGGAA GGCAGGGGTT GTGACACCCC AAGTCAAAGG CGGTGCTGTA
    ACCCCAGCCA GCAGGGCCAA GAAGCCAGAA GAGGTCTCAG AGAGCAGCGA GGAGTCCGAT
    GAGAGTGAGG AGGAGGCCCC GGTGAAGGCC TTGGAGAAAA CCACCCCAAT CAGAGTTGCT
    CCTAGTCCTG CCAAGGGGAC CCCTGGGAAA GGGGCCACCC CAGCAGCCCC TGGGAAGGCA
    GGGCCCTTAG GGAAGCCAGA GGAGGACTCG GAGAGCAGCA GCGAGGAGTC AGACAGCGAG
    GAGGAGACAC AAGCTGTCAA GACCCCAGTG CAGGTGGGAC CTGGAGAGAG GGCCCCGGAG
    CTGGCACCCA CCCCAGGAGG CCTCCTGAGG GCTGATTCTC CCACTCCACG TGCCCTGGCC
    ACGCAAGGGC CACGTGGAGT CTGTGGTGTC TTTGACCCCG TCTGCTATCC TGTTTCCTCA
    AGTCCACTGT CTGGGCTCTC TCCACTGATT CTGTTTCTCT CTCTTCAGGC AAAGCCCTCT
    GGGAAGATCC CCCAGGTTAA AGCTGCCTCT GCAGCACCCA CCCAGGTGCT TTCCCCTAAG
    AAAGGGGCCC CTCCAGCACC CGCTGGCAAG GCAGGGCCTG CAGCCACCCA AGCCCAGCCC
    AGGAAGCAGG AGAAGGAGGA GAGCAGCAGC AGCAGCAGCG AGGAATCAGA CAGCGAGGGC
    GAGGCGCCCA CGGCCACGCG GCAGACTACA AGCCTGGCTC AGGCAAAGCC CTTGGAGAAA
    AACTCTCAGG TCAGAGCTGC CTCAGCCACG GGTGCAGGAC CCTCGGCGAA GGGCACAGTC
    CCTGCGCCCC CTCAGAAGGC AAGGTCTGTG GCCGCCCAGG ACGGGAAGCA GGAGGACTCG
    GAGAGCAGCA GTGAGGAGGA GTCGGACAGC GAGGAGGAGA TGCAGAGGGC AGGGACCTCA
    GCCCAGGCAC AGTCCTCGGG GAAAGCCCTC CAGGTCAGAC CTTCCTCAGG TCCCACCAAG
    GGGCCCCCTC AGAAGGCCGG GCCTGCAACC ACCCAGGTCA AGGTCGAAAG GGCCGAGGAA
    GACTCGGAGA GCAGTGAGGA GGAATCAGAC AGTGAGGACG AAGCACCTGT AGCCAAGACT
    CCAGCCCAGG CAAAATCAAC TGTGAAAACT TCTCAGATCA AGGCCTCCCC AAGGAAGGGC
    ACCCCCATTA CACCCACATC TGCCAGGGTC CCCCCAGTGC AAGTCGGCAC ACCATCCCCC
    CGGACAGCAG CAACAGTGAG TACGCCCACC TGTGCATCAT CCCCAGCCAT AGTCAGGCAC
    ACCCAGAAGC CAGGGGAGGA CTCTTCAAGC AGCGAGGAGT CTGAGAGCGA GGAGGAGACA
    GCTCCTGCTG CAACAGGGGG ACAGATGAAG TCTGTAGGGA AGAGCCTCCC AGTGAAAGCT
    GCCTCAGCAT CCACCAAGGG GCCCTCAGGA AAAGGGACCA CCCCAGGGCC CCCTGGGAAG
    GCAGGACCCC CCGTGGCACA GGTCAAGGCT GAGGTGCAGG AAGACTCAGA GAGCAGTGAG
    GAGTCGGACA GTGAGGAAGC GGCTGCAGCT CCCGCACAGG TGAAGACTTC AGTGAAAACC
    CCTCAGACCA AAGCCAACCC TTCTGCCACC AGAGTGGCTT CAGCCAAAGC AGCAGCACCA
    GCTCCTGGAA AGGGGGGCCT TGCTGTTGCT CAAGCCAAAG TGGGGTCCCC AGCCAAGGTA
    ACCCCAGCAG CGAGAGCCCC CCAGAGCAGC GCCGTCTCTG GGAGCGGCCA GGTGTCCATG
    CCGGCTGTGG GGAAGGCAGC GGCTGCAGCA GCCCAGGCCC AGCCGGGGCC TGTCGGGGGC
    TCACAGGAGG ACTCGGAGAG CAGTGAGGAG GAGTCGGACA GTGAGGGAGA GGCACCTCCT
    CAGGCGAAGC CCTCAGGAAA GACCCCCCAG GTCAAAACTG CCTCAGCCCC CACCAAGGCG
    TGCCCCAAGA AAGGGGCTGC CCCAGTACCC CCTGGGAAGG CAGGACCCCC AGCCGCCCAG
    GCCAGGAGGC AGGAGGAGGA CTCGGAGAGC AGCAGCGAGG AGGAGTCAGA CAGTGACGGG
    GACGTGCCAG CAGCTGCACC TCCAGCCCAG AAGGAGGGTA GCTCCAAAAC TGCCACAAGC
    AAGACCCTGA CTCCAGCATC CCTGGAGAAG AAAGCAGAGG AGTCCTCAAC CAGCAGTGAT
    GAGGACCTGC CGTCGAACCA GGTGATTAAA ACCCCTCTGA TTTTTGTCGA CCCTAATCGT
    AGTCCAGCTA GCCCAGCTGC TACCCCCACC CAAGCGCAGG CTACAGGCAC CCCAAGGAAG
    GCTCAGGCCT TGGAGAGCAC AACCAGGAGC TCCTCTGAGA GCGAGGACGA GGACGTGATC
    CCCGCTACAC AGTGCCCGAC TCCTGCCACT AGGACCAGCG TGGTGACTGT GCCCACAGCA
    CCTCCAAAGG CGGCCCTCAG AGCCAGTGTG GTGGGGGCTC CCAGCAGTGA GGACACAAGT
    CAGGGGTCTG AAGGCAAGAA GCAGGAGGCC TCAGCCACTC AGGTGACCAA GAGGAACCTC
    CCACTGACGC AGGCTGCCCT GAAGGTTCTT GCCCAGAAAG CCAGTGAGGC CCAGCCTCCT
    GCTGCCAAGA CCCCGTCTTC AAGTGGGACT GACAGTGCTG TGGGAACTCT CTCCACGGTG
    AGTCCCCAGA GCACCCCTAC CCAGGTCAGA ATGGCCAACA AGCTCAGAAA GCCTGAGGTT
    CCTGCAGCTC AGCGGGCCAC AGCTACTCCC TCGGGGCACC CCACACCCAA GGCTCCTGCA
    ACCTCAGATG ACAGCAATAG CAGCTCTTCG GGAAGCGAGG ACGAAGCCAA GGGGTCCCAG
    GCGGCCCCGT CAGCCCACAG GCCAGGCTCA GCCCCCTCCA GGAGGGAGAC CTTGGTGGAA
    GAGACCACAA CAGAGTCCAG TGACGATGAG GTGGTGGCAC CTTCCCAGTC TCTCCTCTCA
    GGTTTTGTGA CTCCTGGGCT GACTCCAGCT GGTTCCAAGA CTTCAAAGGC CACTCCCAAA
    CCAGACACCA GCCCCTCTGT TTCCTCTACT CCGGCCACCA GAGATGCCGC AGAAGGCAAT
    CAGGAAGTGG AGCCCCAGCA AGCAGCAGGC ACCATGTCCC CTAAAACAGG CAGAAAAGAG
    GCTGGTGCCA CACCTCAGAA GCCCGGGAAG GGGCCTGGGA GTACCCCGGC CTCCATGCTG
    GCGCTGCAGA GCAACATCAC CCAGCGCCTC CTGAGCGAGC CCTGGCCCCT GAGTGAGGCG
    CAGGTGCAGG CCTCGGTGGC AAAAGTGCTC ACGGAGCTGC TGGAGCAGGA GAGGAAGAAG
    GCCGTGGAGA CCGCCAAGGA AGGCAACCGG AAGGGCCGCC TGGGCCATAA GCGGAAGCTG
    TCCGAAGACC AGACAGCCCC CAAGGCCCCC AAGAGCAAGA AAAAGAAGCA GTTGGCAGCT
    GCAGATAGTG GGGAGCATGT GGTCTCCTCA GAAAAGGCGC CCAGGACTGT CAAAGGGAAA
    TCCAAGAAGG ACAGAGCAAG TGGTGACGCC AAGGAGAAGA AGGAGAAGGA GTCTCCCGGC
    TCTCAGGGGG CCAAGGAGAA GCCAGTAGGG GAGCTGGGGA CTCCGAAGAG TGACGGGGGA
    GACCACGGCA ACCTTAAGAT CAAGAAAGAG AAGAAGAAAT CTGACAAGAA AAAAAAAGAC
    AAGGAGAAAA AGGAAAAGAA GAAGAAAACA AAAAAGGCCT CCACCAAAGA CCCTGGCTCA
    CCATCACAGA AGAAAAAGAA GAGAAAGAAG AAGACAGCAG AGCAGACTGT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011968793.1
    CDS48..4601
    Translation

    Target ORF information:

    RefSeq Version XM_012113403.2
    Organism Ovis aries musimon(Ovis orientalis musimon)
    Definition Ovis aries musimon Treacher Collins-Franceschetti syndrome 1 (TCOF1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012113403.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGCTGAGG CCAGGAAGCG GCGGGAGCTC CTTCCCCTGA TCTACCAGCA TCTGCTGCAG 
    GCGGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA AATTTTCCTG
    AGTCAGCCTG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACTTC GGAGATTGGC
    CGGAAACGGA AGGCAGAGGA AGATGCGGCC TTGCAGGCCA AGAGGACCCG CGTGTCAGAC
    CCCATCAGCA GCTCAGAGAG CTCGGAAGAG GAGGAGGAGG AGGAGGAGGC AGAAGCCAAA
    ACCACCAAAC CCACCCCGAG ATTAGCAGCT ACCAACTCCT CAGTCACAGG GACAGTTTCG
    TCTTCAAATG TGAAAGAAAA AGCCAAGGCA AAGACCACGA AAGCCAGCAA GACGGTGAAC
    TCCACGACAC ACCCTGCCCC GGGAAAGGCA GTGGCCCACC TCCTCACTGG GAAGTCACCC
    CAGAAGACGA CGGGGCCCTC GGCAAATGCT GTCTTGGTGT CAGAAACTGA GGAGGAGGGC
    AGCGTGTCAG CTCTCAGAAC CGCTGCCACG CCTGGAATGG CATCTGCCGA CCAGGCTGAT
    AGCTCCAGTG AGGATACCAC CAGCTCGAGC GATGAGACGG ACTTGGAGGT GAAAGCCGCA
    GTAAAACCAC TGCAGGTCAG AGCCTCAGCC ACTGCAGTCA AGGAGTCTCC GAGAAAAAGT
    GCAGCCCCAA CTCCAGGGAA GGCAGGGGTT GTGACACCCC AAGTCAAAGG CGGTGCTGTA
    ACCCCAGCCA GCAGGGCCAA GAAGCCAGAA GAGGTCTCAG AGAGCAGCGA GGAGTCCGAT
    GAGAGTGAGG AGGAGGCCCC GGTGAAGGCC TTGGAGAAAA CCACCCCAAT CAGAGTTGCT
    CCTAGTCCTG CCAAGGGGAC CCCTGGGAAA GGGGCCACCC CAGCAGCCCC TGGGAAGGCA
    GGGCCCTTAG GGAAGCCAGA GGAGGACTCG GAGAGCAGCA GCGAGGAGTC AGACAGCGAG
    GAGGAGACAC AAGCTGTCAA GACCCCAGTG CAGGTGGGAC CTGGAGAGAG GGCCCCGGAG
    CTGGCACCCA CCCCAGGAGG CCTCCTGAGG GCTGATTCTC CCACTCCACG TGCCCTGGCC
    ACGCAAGGGC CACGTGGAGT CTGTGGTGTC TTTGACCCCG TCTGCTATCC TGTTTCCTCA
    AGTCCACTGT CTGGGCTCTC TCCACTGATT CTGTTTCTCT CTCTTCAGGC AAAGCCCTCT
    GGGAAGATCC CCCAGGTTAA AGCTGCCTCT GCAGCACCCA CCCAGGTGCT TTCCCCTAAG
    AAAGGGGCCC CTCCAGCACC CGCTGGCAAG GCAGGGCCTG CAGCCACCCA AGCCCAGCCC
    AGGAAGCAGG AGAAGGAGGA GAGCAGCAGC AGCAGCAGCG AGGAATCAGA CAGCGAGGGC
    GAGGCGCCCA CGGCCACGCG GCAGACTACA AGCCTGGCTC AGGCAAAGCC CTTGGAGAAA
    AACTCTCAGG TCAGAGCTGC CTCAGCCACG GGTGCAGGAC CCTCGGCGAA GGGCACAGTC
    CCTGCGCCCC CTCAGAAGGC AAGGTCTGTG GCCGCCCAGG ACGGGAAGCA GGAGGACTCG
    GAGAGCAGCA GTGAGGAGGA GTCGGACAGC GAGGAGGAGA TGCAGAGGGC AGGGACCTCA
    GCCCAGGCAC AGTCCTCGGG GAAAGCCCTC CAGGTCAGAC CTTCCTCAGG TCCCACCAAG
    GGGCCCCCTC AGAAGGCCGG GCCTGCAACC ACCCAGGTCA AGGTCGAAAG GGCCGAGGAA
    GACTCGGAGA GCAGTGAGGA GGAATCAGAC AGTGAGGACG AAGCACCTGT AGCCAAGACT
    CCAGCCCAGG CAAAATCAAC TGTGAAAACT TCTCAGATCA AGGCCTCCCC AAGGAAGGGC
    ACCCCCATTA CACCCACATC TGCCAGGGTC CCCCCAGTGC AAGTCGGCAC ACCATCCCCC
    CGGACAGCAG CAACAGTGAG TACGCCCACC TGTGCATCAT CCCCAGCCAT AGTCAGGCAC
    ACCCAGAAGC CAGGGGAGGA CTCTTCAAGC AGCGAGGAGT CTGAGAGCGA GGAGGAGACA
    GCTCCTGCTG CAACAGGGGG ACAGATGAAG TCTGTAGGGA AGAGCCTCCC AGTGAAAGCT
    GCCTCAGCAT CCACCAAGGG GCCCTCAGGA AAAGGGACCA CCCCAGGGCC CCCTGGGAAG
    GCAGGACCCC CCGTGGCACA GGTCAAGGCT GAGGTGCAGG AAGACTCAGA GAGCAGTGAG
    GAGTCGGACA GTGAGGAAGC GGCTGCAGCT CCCGCACAGG TGAAGACTTC AGTGAAAACC
    CCTCAGACCA AAGCCAACCC TTCTGCCACC AGAGTGGCTT CAGCCAAAGC AGCAGCACCA
    GCTCCTGGAA AGGGGGGCCT TGCTGTTGCT CAAGCCAAAG TGGGGTCCCC AGCCAAGGTA
    ACCCCAGCAG CGAGAGCCCC CCAGAGCAGC GCCGTCTCTG GGAGCGGCCA GGTGTCCATG
    CCGGCTGTGG GGAAGGCAGC GGCTGCAGCA GCCCAGGCCC AGCCGGGGCC TGTCGGGGGC
    TCACAGGAGG ACTCGGAGAG CAGTGAGGAG GAGTCGGACA GTGAGGGAGA GGCACCTCCT
    CAGGCGAAGC CCTCAGGAAA GACCCCCCAG GTCAAAACTG CCTCAGCCCC CACCAAGGCG
    TGCCCCAAGA AAGGGGCTGC CCCAGTACCC CCTGGGAAGG CAGGACCCCC AGCCGCCCAG
    GCCAGGAGGC AGGAGGAGGA CTCGGAGAGC AGCAGCGAGG AGGAGTCAGA CAGTGACGGG
    GACGTGCCAG CAGCTGCACC TCCAGCCCAG AAGGAGGGTA GCTCCAAAAC TGCCACAAGC
    AAGACCCTGA CTCCAGCATC CCTGGAGAAG AAAGCAGAGG AGTCCTCAAC CAGCAGTGAT
    GAGGACCTGC CGTCGAACCA GGTGATTAAA ACCCCTCTGA TTTTTGTCGA CCCTAATCGT
    AGTCCAGCTA GCCCAGCTGC TACCCCCACC CAAGCGCAGG CTACAGGCAC CCCAAGGAAG
    GCTCAGGCCT TGGAGAGCAC AACCAGGAGC TCCTCTGAGA GCGAGGACGA GGACGTGATC
    CCCGCTACAC AGTGCCCGAC TCCTGCCACT AGGACCAGCG TGGTGACTGT GCCCACAGCA
    CCTCCAAAGG CGGCCCTCAG AGCCAGTGTG GTGGGGGCTC CCAGCAGTGA GGACACAAGT
    CAGGGGTCTG AAGGCAAGAA GCAGGAGGCC TCAGCCACTC AGGTGACCAA GAGGAACCTC
    CCACTGACGC AGGCTGCCCT GAAGGTTCTT GCCCAGAAAG CCAGTGAGGC CCAGCCTCCT
    GCTGCCAAGA CCCCGTCTTC AAGTGGGACT GACAGTGCTG TGGGAACTCT CTCCACGGTG
    AGTCCCCAGA GCACCCCTAC CCAGGTCAGA ATGGCCAACA AGCTCAGAAA GCCTGAGGTT
    CCTGCAGCTC AGCGGGCCAC AGCTACTCCC TCGGGGCACC CCACACCCAA GGCTCCTGCA
    ACCTCAGATG ACAGCAATAG CAGCTCTTCG GGAAGCGAGG ACGAAGCCAA GGGGTCCCAG
    GCGGCCCCGT CAGCCCACAG GCCAGGCTCA GCCCCCTCCA GGAGGGAGAC CTTGGTGGAA
    GAGACCACAA CAGAGTCCAG TGACGATGAG GTGGTGGCAC CTTCCCAGTC TCTCCTCTCA
    GGTTTTGTGA CTCCTGGGCT GACTCCAGCT GGTTCCAAGA CTTCAAAGGC CACTCCCAAA
    CCAGACACCA GCCCCTCTGT TTCCTCTACT CCGGCCACCA GAGATGCCGC AGAAGGCAAT
    CAGGAAGTGG AGCCCCAGCA AGCAGCAGGC ACCATGTCCC CTAAAACAGG CAGAAAAGAG
    GCTGGTGCCA CACCTCAGAA GCCCGGGAAG GGGCCTGGGA GTACCCCGGC CTCCATGCTG
    GCGCTGCAGA GCAACATCAC CCAGCGCCTC CTGAGCGAGC CCTGGCCCCT GAGTGAGGCG
    CAGGTGCAGG CCTCGGTGGC AAAAGTGCTC ACGGAGCTGC TGGAGCAGGA GAGGAAGAAG
    GCCGTGGAGA CCGCCAAGGA AGGCAACCGG AAGGGCCGCC TGGGCCATAA GCGGAAGCTG
    TCCGAAGACC AGACAGCCCC CAAGGCCCCC AAGAGCAAGA AAAAGAAGCA GTTGGCAGCT
    GCAGATAGTG GGGAGCATGT GGTCTCCTCA GAAAAGGCGC CCAGGACTGT CAAAGGGAAA
    TCCAAGAAGG ACAGAGCAAG TGGTGACGCC AAGGAGAAGA AGGAGAAGGA GTCTCCCGGC
    TCTCAGGGGG CCAAGGAGAA GCCAGTAGGG GAGCTGGGGA CTCCGAAGAG TGACGGGGGA
    GACCACGGCA ACCTTAAGAT CAAGAAAGAG AAGAAGAAAT CTGACAAGAA AAAAAAAGAC
    AAGGAGAAAA AGGAAAAGAA GAAGAAAACA AAAAAGGCCT CCACCAAAGA CCCTGGCTCA
    CCATCACAGA AGAAAAAGAA GAGAAAGAAG AAGACAGCAG AGCAGACTGT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OOv03147
    Clone ID Related Accession (Same CDS sequence) XM_012113404.2
    Accession Version XM_012113404.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4545bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-16
    Organism Ovis aries musimon(Ovis orientalis musimon)
    Product treacle protein isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942253.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012113404.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGCTGAGG CCAGGAAGCG GCGGGAGCTC CTTCCCCTGA TCTACCAGCA TCTGCTGCAG 
    GCGGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA AATTTTCCTG
    AGTCAGCCTG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACTTC GGAGATTGGC
    CGGAAACGGA AGGCAGAGGA AGATGCGGCC TTGCAGGCCA AGAGGACCCG CGTGTCAGAC
    CCCATCAGCA GCTCAGAGAG CTCGGAAGAG GAGGAGGAGG AGGAGGAGGC AGAAGCCAAA
    ACCACCAAAC CCACCCCGAG ATTAGCAGCT ACCAACTCCT CAGTCACAGG GACAGTTTCG
    TCTTCAAATG TGAAAGAAAA AGCCAAGGCA AAGACCACGA AAGCCAGCAA GACGGTGAAC
    TCCACGACAC ACCCTGCCCC GGGAAAGGCA GTGGCCCACC TCCTCACTGG GAAGTCACCC
    CAGAAGACGA CGGGGCCCTC GGCAAATGCT GTCTTGGTGT CAGAAACTGA GGAGGAGGGC
    AGCGTGTCAG CTCTCAGAAC CGCTGCCACG CCTGGAATGG CATCTGCCGA CCAGGCTGAT
    AGCTCCAGTG AGGATACCAC CAGCTCGAGC GATGAGACGG ACTTGGAGGT GAAAGCCGCA
    GTAAAACCAC TGCAGGTCAG AGCCTCAGCC ACTGCAGTCA AGGAGTCTCC GAGAAAAAGT
    GCAGCCCCAA CTCCAGGGAA GGCAGGGGTT GTGACACCCC AAGTCAAAGG CGGTGCTGTA
    ACCCCAGCCA GCAGGGCCAA GAAGCCAGAA GAGGTCTCAG AGAGCAGCGA GGAGTCCGAT
    GAGAGTGAGG AGGAGGCCCC GGTGAAGGCC TTGGAGAAAA CCACCCCAAT CAGAGTTGCT
    CCTAGTCCTG CCAAGGGGAC CCCTGGGAAA GGGGCCACCC CAGCAGCCCC TGGGAAGGCA
    GGGCCCTTAG GGAAGCCAGA GGAGGACTCG GAGAGCAGCA GCGAGGAGTC AGACAGCGAG
    GAGGAGACAC AAGCTGTCAA GACCCCAGTG CAGGTGGGAC CTGGAGAGAG GGCCCCGGAG
    CTGGCACCCA CCCCAGGAGG CCTCCTGAGG GCTGATTCTC CCACTCCACG TGCCCTGGCC
    ACGCAAGGGC CACGTGGAGT CTGTGGTGTC TTTGACCCCG TCTGCTATCC TGTTTCCTCA
    AGTCCACTGT CTGGGCTCTC TCCACTGATT CTGTTTCTCT CTCTTCAGGC AAAGCCCTCT
    GGGAAGATCC CCCAGGTTAA AGCTGCCTCT GCAGCACCCA CCCAGGTGCT TTCCCCTAAG
    AAAGGGGCCC CTCCAGCACC CGCTGGCAAG GCAGGGCCTG CAGCCACCCA AGCCCAGCCC
    AGGAAGCAGG AGAAGGAGGA GAGCAGCAGC AGCAGCAGCG AGGAATCAGA CAGCGAGGGC
    GAGGCGCCCA CGGCCACGCG GCAGACTACA AGCCTGGCTC AGGCAAAGCC CTTGGAGAAA
    AACTCTCAGG TCAGAGCTGC CTCAGCCACG GGTGCAGGAC CCTCGGCGAA GGGCACAGTC
    CCTGCGCCCC CTCAGAAGGC AAGGTCTGTG GCCGCCCAGG ACGGGAAGCA GGAGGACTCG
    GAGAGCAGCA GTGAGGAGGA GTCGGACAGC GAGGAGGAGA TGCAGAGGGC AGGGACCTCA
    GCCCAGGCAC AGTCCTCGGG GAAAGCCCTC CAGGTCAGAC CTTCCTCAGG TCCCACCAAG
    GGGCCCCCTC AGAAGGCCGG GCCTGCAACC ACCCAGGTCA AGGTCGAAAG GGCCGAGGAA
    GACTCGGAGA GCAGTGAGGA GGAATCAGAC AGTGAGGACG AAGCACCTGT AGCCAAGACT
    CCAGCCCAGG CAAAATCAAC TGTGAAAACT TCTCAGATCA AGGCCTCCCC AAGGAAGGGC
    ACCCCCATTA CACCCACATC TGCCAGGGTC CCCCCAGTGC AAGTCGGCAC ACCATCCCCC
    CGGACAGCAG CAACAATGAA GTCTGTAGGG AAGAGCCTCC CAGTGAAAGC TGCCTCAGCA
    TCCACCAAGG GGCCCTCAGG AAAAGGGACC ACCCCAGGGC CCCCTGGGAA GGCAGGACCC
    CCCGTGGCAC AGGTCAAGGC TGAGGTGCAG GAAGACTCAG AGAGCAGTGA GGAGTCGGAC
    AGTGAGGAAG CGGCTGCAGC TCCCGCACAG GTGAAGACTT CAGTGAAAAC CCCTCAGACC
    AAAGCCAACC CTTCTGCCAC CAGAGTGGCT TCAGCCAAAG CAGCAGCACC AGCTCCTGGA
    AAGGGGGGCC TTGCTGTTGC TCAAGCCAAA GTGGGGTCCC CAGCCAAGGT AACCCCAGCA
    GCGAGAGCCC CCCAGAGCAG CGCCGTCTCT GGGAGCGGCC AGGTGTCCAT GCCGGCTGTG
    GGGAAGGCAG CGGCTGCAGC AGCCCAGGCC CAGCCGGGGC CTGTCGGGGG CTCACAGGAG
    GACTCGGAGA GCAGTGAGGA GGAGTCGGAC AGTGAGGGAG AGGCACCTCC TCAGGCGAAG
    CCCTCAGGAA AGACCCCCCA GGTCAAAACT GCCTCAGCCC CCACCAAGGC GTGCCCCAAG
    AAAGGGGCTG CCCCAGTACC CCCTGGGAAG GCAGGACCCC CAGCCGCCCA GGCCAGGAGG
    CAGGAGGAGG ACTCGGAGAG CAGCAGCGAG GAGGAGTCAG ACAGTGACGG GGACGTGCCA
    GCAGCTGCAC CTCCAGCCCA GAAGGAGGGT AGCTCCAAAA CTGCCACAAG CAAGACCCTG
    ACTCCAGCAT CCCTGGAGAA GAAAGCAGAG GAGTCCTCAA CCAGCAGTGA TGAGGACCTG
    CCGTCGAACC AGGTGATTAA AACCCCTCTG ATTTTTGTCG ACCCTAATCG TAGTCCAGCT
    AGCCCAGCTG CTACCCCCAC CCAAGCGCAG GCTACAGGCA CCCCAAGGAA GGCTCAGGCC
    TTGGAGAGCA CAACCAGGAG CTCCTCTGAG AGCGAGGACG AGGACGTGAT CCCCGCTACA
    CAGTGCCCGA CTCCTGCCAC TAGGACCAGC GTGGTGACTG TGCCCACAGC ACCTCCAAAG
    GCGGCCCTCA GAGCCAGTGT GGTGGGGGCT CCCAGCAGTG AGGACACAAG TCAGGGGTCT
    GAAGGCAAGA AGCAGGAGGC CTCAGCCACT CAGGTGACCA AGAGGAACCT CCCACTGACG
    CAGGCTGCCC TGAAGGTTCT TGCCCAGAAA GCCAGTGAGG CCCAGCCTCC TGCTGCCAAG
    ACCCCGTCTT CAAGTGGGAC TGACAGTGCT GTGGGAACTC TCTCCACGGT GAGTCCCCAG
    AGCACCCCTA CCCAGGTCAG AATGGCCAAC AAGCTCAGAA AGCCTGAGGT TCCTGCAGCT
    CAGCGGGCCA CAGCTACTCC CTCGGGGCAC CCCACACCCA AGGCTCCTGC AACCTCAGAT
    GACAGCAATA GCAGCTCTTC GGGAAGCGAG GACGAAGCCA AGGGGTCCCA GGCGGCCCCG
    TCAGCCCACA GGCCAGGCTC AGCCCCCTCC AGGAGGGAGA CCTTGGTGGA AGAGACCACA
    ACAGAGTCCA GTGACGATGA GGTGGTGGCA CCTTCCCAGT CTCTCCTCTC AGGTTTTGTG
    ACTCCTGGGC TGACTCCAGC TGGTTCCAAG ACTTCAAAGG CCACTCCCAA ACCAGACACC
    AGCCCCTCTG TTTCCTCTAC TCCGGCCACC AGAGATGCCG CAGAAGGCAA TCAGGAAGTG
    GAGCCCCAGC AAGCAGCAGG CACCATGTCC CCTAAAACAG GCAGAAAAGA GGCTGGTGCC
    ACACCTCAGA AGCCCGGGAA GGGGCCTGGG AGTACCCCGG CCTCCATGCT GGCGCTGCAG
    AGCAACATCA CCCAGCGCCT CCTGAGCGAG CCCTGGCCCC TGAGTGAGGC GCAGGTGCAG
    GCCTCGGTGG CAAAAGTGCT CACGGAGCTG CTGGAGCAGG AGAGGAAGAA GGCCGTGGAG
    ACCGCCAAGG AAGGCAACCG GAAGGGCCGC CTGGGCCATA AGCGGAAGCT GTCCGAAGAC
    CAGACAGCCC CCAAGGCCCC CAAGAGCAAG AAAAAGAAGC AGTTGGCAGC TGCAGATAGT
    GGGGAGCATG TGGTCTCCTC AGAAAAGGCG CCCAGGACTG TCAAAGGGAA ATCCAAGAAG
    GACAGAGCAA GTGGTGACGC CAAGGAGAAG AAGGAGAAGG AGTCTCCCGG CTCTCAGGGG
    GCCAAGGAGA AGCCAGTAGG GGAGCTGGGG ACTCCGAAGA GTGACGGGGG AGACCACGGC
    AACCTTAAGA TCAAGAAAGA GAAGAAGAAA TCTGACAAGA GATGGGCAAA GCCAGAACCA
    GGTCCTGGAG CAGCGGAGCC ACCACCACCA GGACGGCGCT CACAGCTCCA GGGCATGTTC
    CGCTCGAAGA GCCCTGCCCT CCCCCACGGC GGCCACAGCC ACGTGCACTG GCAGCCGAGG
    CTCCTGGGGT CCAGGCTCCT GGATAGCAGC TGCCCCTTGC TGCATTCTGT GCTGGAGTCC
    TCGTGCAGCC GGGCCACGTT TAACCCCCAC CCCAGCCCTG TCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011968794.1
    CDS48..4592
    Translation

    Target ORF information:

    RefSeq Version XM_012113404.2
    Organism Ovis aries musimon(Ovis orientalis musimon)
    Definition Ovis aries musimon Treacher Collins-Franceschetti syndrome 1 (TCOF1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012113404.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGCTGAGG CCAGGAAGCG GCGGGAGCTC CTTCCCCTGA TCTACCAGCA TCTGCTGCAG 
    GCGGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA AATTTTCCTG
    AGTCAGCCTG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACTTC GGAGATTGGC
    CGGAAACGGA AGGCAGAGGA AGATGCGGCC TTGCAGGCCA AGAGGACCCG CGTGTCAGAC
    CCCATCAGCA GCTCAGAGAG CTCGGAAGAG GAGGAGGAGG AGGAGGAGGC AGAAGCCAAA
    ACCACCAAAC CCACCCCGAG ATTAGCAGCT ACCAACTCCT CAGTCACAGG GACAGTTTCG
    TCTTCAAATG TGAAAGAAAA AGCCAAGGCA AAGACCACGA AAGCCAGCAA GACGGTGAAC
    TCCACGACAC ACCCTGCCCC GGGAAAGGCA GTGGCCCACC TCCTCACTGG GAAGTCACCC
    CAGAAGACGA CGGGGCCCTC GGCAAATGCT GTCTTGGTGT CAGAAACTGA GGAGGAGGGC
    AGCGTGTCAG CTCTCAGAAC CGCTGCCACG CCTGGAATGG CATCTGCCGA CCAGGCTGAT
    AGCTCCAGTG AGGATACCAC CAGCTCGAGC GATGAGACGG ACTTGGAGGT GAAAGCCGCA
    GTAAAACCAC TGCAGGTCAG AGCCTCAGCC ACTGCAGTCA AGGAGTCTCC GAGAAAAAGT
    GCAGCCCCAA CTCCAGGGAA GGCAGGGGTT GTGACACCCC AAGTCAAAGG CGGTGCTGTA
    ACCCCAGCCA GCAGGGCCAA GAAGCCAGAA GAGGTCTCAG AGAGCAGCGA GGAGTCCGAT
    GAGAGTGAGG AGGAGGCCCC GGTGAAGGCC TTGGAGAAAA CCACCCCAAT CAGAGTTGCT
    CCTAGTCCTG CCAAGGGGAC CCCTGGGAAA GGGGCCACCC CAGCAGCCCC TGGGAAGGCA
    GGGCCCTTAG GGAAGCCAGA GGAGGACTCG GAGAGCAGCA GCGAGGAGTC AGACAGCGAG
    GAGGAGACAC AAGCTGTCAA GACCCCAGTG CAGGTGGGAC CTGGAGAGAG GGCCCCGGAG
    CTGGCACCCA CCCCAGGAGG CCTCCTGAGG GCTGATTCTC CCACTCCACG TGCCCTGGCC
    ACGCAAGGGC CACGTGGAGT CTGTGGTGTC TTTGACCCCG TCTGCTATCC TGTTTCCTCA
    AGTCCACTGT CTGGGCTCTC TCCACTGATT CTGTTTCTCT CTCTTCAGGC AAAGCCCTCT
    GGGAAGATCC CCCAGGTTAA AGCTGCCTCT GCAGCACCCA CCCAGGTGCT TTCCCCTAAG
    AAAGGGGCCC CTCCAGCACC CGCTGGCAAG GCAGGGCCTG CAGCCACCCA AGCCCAGCCC
    AGGAAGCAGG AGAAGGAGGA GAGCAGCAGC AGCAGCAGCG AGGAATCAGA CAGCGAGGGC
    GAGGCGCCCA CGGCCACGCG GCAGACTACA AGCCTGGCTC AGGCAAAGCC CTTGGAGAAA
    AACTCTCAGG TCAGAGCTGC CTCAGCCACG GGTGCAGGAC CCTCGGCGAA GGGCACAGTC
    CCTGCGCCCC CTCAGAAGGC AAGGTCTGTG GCCGCCCAGG ACGGGAAGCA GGAGGACTCG
    GAGAGCAGCA GTGAGGAGGA GTCGGACAGC GAGGAGGAGA TGCAGAGGGC AGGGACCTCA
    GCCCAGGCAC AGTCCTCGGG GAAAGCCCTC CAGGTCAGAC CTTCCTCAGG TCCCACCAAG
    GGGCCCCCTC AGAAGGCCGG GCCTGCAACC ACCCAGGTCA AGGTCGAAAG GGCCGAGGAA
    GACTCGGAGA GCAGTGAGGA GGAATCAGAC AGTGAGGACG AAGCACCTGT AGCCAAGACT
    CCAGCCCAGG CAAAATCAAC TGTGAAAACT TCTCAGATCA AGGCCTCCCC AAGGAAGGGC
    ACCCCCATTA CACCCACATC TGCCAGGGTC CCCCCAGTGC AAGTCGGCAC ACCATCCCCC
    CGGACAGCAG CAACAATGAA GTCTGTAGGG AAGAGCCTCC CAGTGAAAGC TGCCTCAGCA
    TCCACCAAGG GGCCCTCAGG AAAAGGGACC ACCCCAGGGC CCCCTGGGAA GGCAGGACCC
    CCCGTGGCAC AGGTCAAGGC TGAGGTGCAG GAAGACTCAG AGAGCAGTGA GGAGTCGGAC
    AGTGAGGAAG CGGCTGCAGC TCCCGCACAG GTGAAGACTT CAGTGAAAAC CCCTCAGACC
    AAAGCCAACC CTTCTGCCAC CAGAGTGGCT TCAGCCAAAG CAGCAGCACC AGCTCCTGGA
    AAGGGGGGCC TTGCTGTTGC TCAAGCCAAA GTGGGGTCCC CAGCCAAGGT AACCCCAGCA
    GCGAGAGCCC CCCAGAGCAG CGCCGTCTCT GGGAGCGGCC AGGTGTCCAT GCCGGCTGTG
    GGGAAGGCAG CGGCTGCAGC AGCCCAGGCC CAGCCGGGGC CTGTCGGGGG CTCACAGGAG
    GACTCGGAGA GCAGTGAGGA GGAGTCGGAC AGTGAGGGAG AGGCACCTCC TCAGGCGAAG
    CCCTCAGGAA AGACCCCCCA GGTCAAAACT GCCTCAGCCC CCACCAAGGC GTGCCCCAAG
    AAAGGGGCTG CCCCAGTACC CCCTGGGAAG GCAGGACCCC CAGCCGCCCA GGCCAGGAGG
    CAGGAGGAGG ACTCGGAGAG CAGCAGCGAG GAGGAGTCAG ACAGTGACGG GGACGTGCCA
    GCAGCTGCAC CTCCAGCCCA GAAGGAGGGT AGCTCCAAAA CTGCCACAAG CAAGACCCTG
    ACTCCAGCAT CCCTGGAGAA GAAAGCAGAG GAGTCCTCAA CCAGCAGTGA TGAGGACCTG
    CCGTCGAACC AGGTGATTAA AACCCCTCTG ATTTTTGTCG ACCCTAATCG TAGTCCAGCT
    AGCCCAGCTG CTACCCCCAC CCAAGCGCAG GCTACAGGCA CCCCAAGGAA GGCTCAGGCC
    TTGGAGAGCA CAACCAGGAG CTCCTCTGAG AGCGAGGACG AGGACGTGAT CCCCGCTACA
    CAGTGCCCGA CTCCTGCCAC TAGGACCAGC GTGGTGACTG TGCCCACAGC ACCTCCAAAG
    GCGGCCCTCA GAGCCAGTGT GGTGGGGGCT CCCAGCAGTG AGGACACAAG TCAGGGGTCT
    GAAGGCAAGA AGCAGGAGGC CTCAGCCACT CAGGTGACCA AGAGGAACCT CCCACTGACG
    CAGGCTGCCC TGAAGGTTCT TGCCCAGAAA GCCAGTGAGG CCCAGCCTCC TGCTGCCAAG
    ACCCCGTCTT CAAGTGGGAC TGACAGTGCT GTGGGAACTC TCTCCACGGT GAGTCCCCAG
    AGCACCCCTA CCCAGGTCAG AATGGCCAAC AAGCTCAGAA AGCCTGAGGT TCCTGCAGCT
    CAGCGGGCCA CAGCTACTCC CTCGGGGCAC CCCACACCCA AGGCTCCTGC AACCTCAGAT
    GACAGCAATA GCAGCTCTTC GGGAAGCGAG GACGAAGCCA AGGGGTCCCA GGCGGCCCCG
    TCAGCCCACA GGCCAGGCTC AGCCCCCTCC AGGAGGGAGA CCTTGGTGGA AGAGACCACA
    ACAGAGTCCA GTGACGATGA GGTGGTGGCA CCTTCCCAGT CTCTCCTCTC AGGTTTTGTG
    ACTCCTGGGC TGACTCCAGC TGGTTCCAAG ACTTCAAAGG CCACTCCCAA ACCAGACACC
    AGCCCCTCTG TTTCCTCTAC TCCGGCCACC AGAGATGCCG CAGAAGGCAA TCAGGAAGTG
    GAGCCCCAGC AAGCAGCAGG CACCATGTCC CCTAAAACAG GCAGAAAAGA GGCTGGTGCC
    ACACCTCAGA AGCCCGGGAA GGGGCCTGGG AGTACCCCGG CCTCCATGCT GGCGCTGCAG
    AGCAACATCA CCCAGCGCCT CCTGAGCGAG CCCTGGCCCC TGAGTGAGGC GCAGGTGCAG
    GCCTCGGTGG CAAAAGTGCT CACGGAGCTG CTGGAGCAGG AGAGGAAGAA GGCCGTGGAG
    ACCGCCAAGG AAGGCAACCG GAAGGGCCGC CTGGGCCATA AGCGGAAGCT GTCCGAAGAC
    CAGACAGCCC CCAAGGCCCC CAAGAGCAAG AAAAAGAAGC AGTTGGCAGC TGCAGATAGT
    GGGGAGCATG TGGTCTCCTC AGAAAAGGCG CCCAGGACTG TCAAAGGGAA ATCCAAGAAG
    GACAGAGCAA GTGGTGACGC CAAGGAGAAG AAGGAGAAGG AGTCTCCCGG CTCTCAGGGG
    GCCAAGGAGA AGCCAGTAGG GGAGCTGGGG ACTCCGAAGA GTGACGGGGG AGACCACGGC
    AACCTTAAGA TCAAGAAAGA GAAGAAGAAA TCTGACAAGA GATGGGCAAA GCCAGAACCA
    GGTCCTGGAG CAGCGGAGCC ACCACCACCA GGACGGCGCT CACAGCTCCA GGGCATGTTC
    CGCTCGAAGA GCCCTGCCCT CCCCCACGGC GGCCACAGCC ACGTGCACTG GCAGCCGAGG
    CTCCTGGGGT CCAGGCTCCT GGATAGCAGC TGCCCCTTGC TGCATTCTGT GCTGGAGTCC
    TCGTGCAGCC GGGCCACGTT TAACCCCCAC CCCAGCCCTG TCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OOv03148
    Clone ID Related Accession (Same CDS sequence) XM_012113405.2
    Accession Version XM_012113405.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4479bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-16
    Organism Ovis aries musimon(Ovis orientalis musimon)
    Product treacle protein isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942253.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012113405.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGCTGAGG CCAGGAAGCG GCGGGAGCTC CTTCCCCTGA TCTACCAGCA TCTGCTGCAG 
    GCGGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA AATTTTCCTG
    AGTCAGCCTG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACTTC GGAGATTGGC
    CGGAAACGGA AGGCAGAGGA AGATGCGGCC TTGCAGGCCA AGAGGACCCG CGTGTCAGAC
    CCCATCAGCA GCTCAGAGAG CTCGGAAGAG GAGGAGGAGG AGGAGGAGGC AGAAGCCAAA
    ACCACCAAAC CCACCCCGAG ATTAGCAGCT ACCAACTCCT CAGTCACAGG GACAGTTTCG
    TCTTCAAATG TGAAAGAAAA AGCCAAGGCA AAGACCACGA AAGCCAGCAA GACGGTGAAC
    TCCACGACAC ACCCTGCCCC GGGAAAGGCA GTGGCCCACC TCCTCACTGG GAAGTCACCC
    CAGAAGACGA CGGGGCCCTC GGCAAATGCT GTCTTGGTGT CAGAAACTGA GGAGGAGGGC
    AGCGTGTCAG CTCTCAGAAC CGCTGCCACG CCTGGAATGG CATCTGCCGA CCAGGCTGAT
    AGCTCCAGTG AGGATACCAC CAGCTCGAGC GATGAGACGG ACTTGGAGGT GAAAGCCGCA
    GTAAAACCAC TGCAGGTCAG AGCCTCAGCC ACTGCAGTCA AGGAGTCTCC GAGAAAAAGT
    GCAGCCCCAA CTCCAGGGAA GGCAGGGGTT GTGACACCCC AAGTCAAAGG CGGTGCTGTA
    ACCCCAGCCA GCAGGGCCAA GAAGCCAGAA GAGGTCTCAG AGAGCAGCGA GGAGTCCGAT
    GAGAGTGAGG AGGAGGCCCC GGTGAAGGCC TTGGAGAAAA CCACCCCAAT CAGAGTTGCT
    CCTAGTCCTG CCAAGGGGAC CCCTGGGAAA GGGGCCACCC CAGCAGCCCC TGGGAAGGCA
    GGGCCCTTAG GGAAGCCAGA GGAGGACTCG GAGAGCAGCA GCGAGGAGTC AGACAGCGAG
    GAGGAGACAC AAGCTGTCAA GACCCCAGTG CAGGCAAAGC CCTCTGGGAA GATCCCCCAG
    GTTAAAGCTG CCTCTGCAGC ACCCACCCAG GTGCTTTCCC CTAAGAAAGG GGCCCCTCCA
    GCACCCGCTG GCAAGGCAGG GCCTGCAGCC ACCCAAGCCC AGCCCAGGAA GCAGGAGAAG
    GAGGAGAGCA GCAGCAGCAG CAGCGAGGAA TCAGACAGCG AGGGCGAGGC GCCCACGGCC
    ACGCGGCAGA CTACAAGCCT GGCTCAGGCA AAGCCCTTGG AGAAAAACTC TCAGGTCAGA
    GCTGCCTCAG CCACGGGTGC AGGACCCTCG GCGAAGGGCA CAGTCCCTGC GCCCCCTCAG
    AAGGCAAGGT CTGTGGCCGC CCAGGACGGG AAGCAGGAGG ACTCGGAGAG CAGCAGTGAG
    GAGGAGTCGG ACAGCGAGGA GGAGATGCAG AGGGCAGGGA CCTCAGCCCA GGCACAGTCC
    TCGGGGAAAG CCCTCCAGGT CAGACCTTCC TCAGGTCCCA CCAAGGGGCC CCCTCAGAAG
    GCCGGGCCTG CAACCACCCA GGTCAAGGTC GAAAGGGCCG AGGAAGACTC GGAGAGCAGT
    GAGGAGGAAT CAGACAGTGA GGACGAAGCA CCTGTAGCCA AGACTCCAGC CCAGGCAAAA
    TCAACTGTGA AAACTTCTCA GATCAAGGCC TCCCCAAGGA AGGGCACCCC CATTACACCC
    ACATCTGCCA GGGTCCCCCC AGTGCAAGTC GGCACACCAT CCCCCCGGAC AGCAGCAACA
    GTGAGTACGC CCACCTGTGC ATCATCCCCA GCCATAGTCA GGCACACCCA GAAGCCAGGG
    GAGGACTCTT CAAGCAGCGA GGAGTCTGAG AGCGAGGAGG AGACAGCTCC TGCTGCAACA
    GGGGGACAGA TGAAGTCTGT AGGGAAGAGC CTCCCAGTGA AAGCTGCCTC AGCATCCACC
    AAGGGGCCCT CAGGAAAAGG GACCACCCCA GGGCCCCCTG GGAAGGCAGG ACCCCCCGTG
    GCACAGGTCA AGGCTGAGGT GCAGGAAGAC TCAGAGAGCA GTGAGGAGTC GGACAGTGAG
    GAAGCGGCTG CAGCTCCCGC ACAGGTGAAG ACTTCAGTGA AAACCCCTCA GACCAAAGCC
    AACCCTTCTG CCACCAGAGT GGCTTCAGCC AAAGCAGCAG CACCAGCTCC TGGAAAGGGG
    GGCCTTGCTG TTGCTCAAGC CAAAGTGGGG TCCCCAGCCA AGGTAACCCC AGCAGCGAGA
    GCCCCCCAGA GCAGCGCCGT CTCTGGGAGC GGCCAGGTGT CCATGCCGGC TGTGGGGAAG
    GCAGCGGCTG CAGCAGCCCA GGCCCAGCCG GGGCCTGTCG GGGGCTCACA GGAGGACTCG
    GAGAGCAGTG AGGAGGAGTC GGACAGTGAG GGAGAGGCAC CTCCTCAGGC GAAGCCCTCA
    GGAAAGACCC CCCAGGTCAA AACTGCCTCA GCCCCCACCA AGGCGTGCCC CAAGAAAGGG
    GCTGCCCCAG TACCCCCTGG GAAGGCAGGA CCCCCAGCCG CCCAGGCCAG GAGGCAGGAG
    GAGGACTCGG AGAGCAGCAG CGAGGAGGAG TCAGACAGTG ACGGGGACGT GCCAGCAGCT
    GCACCTCCAG CCCAGAAGGA GGGTAGCTCC AAAACTGCCA CAAGCAAGAC CCTGACTCCA
    GCATCCCTGG AGAAGAAAGC AGAGGAGTCC TCAACCAGCA GTGATGAGGA CCTGCCGTCG
    AACCAGGTGA TTAAAACCCC TCTGATTTTT GTCGACCCTA ATCGTAGTCC AGCTAGCCCA
    GCTGCTACCC CCACCCAAGC GCAGGCTACA GGCACCCCAA GGAAGGCTCA GGCCTTGGAG
    AGCACAACCA GGAGCTCCTC TGAGAGCGAG GACGAGGACG TGATCCCCGC TACACAGTGC
    CCGACTCCTG CCACTAGGAC CAGCGTGGTG ACTGTGCCCA CAGCACCTCC AAAGGCGGCC
    CTCAGAGCCA GTGTGGTGGG GGCTCCCAGC AGTGAGGACA CAAGTCAGGG GTCTGAAGGC
    AAGAAGCAGG AGGCCTCAGC CACTCAGGTG ACCAAGAGGA ACCTCCCACT GACGCAGGCT
    GCCCTGAAGG TTCTTGCCCA GAAAGCCAGT GAGGCCCAGC CTCCTGCTGC CAAGACCCCG
    TCTTCAAGTG GGACTGACAG TGCTGTGGGA ACTCTCTCCA CGGTGAGTCC CCAGAGCACC
    CCTACCCAGG TCAGAATGGC CAACAAGCTC AGAAAGCCTG AGGTTCCTGC AGCTCAGCGG
    GCCACAGCTA CTCCCTCGGG GCACCCCACA CCCAAGGCTC CTGCAACCTC AGATGACAGC
    AATAGCAGCT CTTCGGGAAG CGAGGACGAA GCCAAGGGGT CCCAGGCGGC CCCGTCAGCC
    CACAGGCCAG GCTCAGCCCC CTCCAGGAGG GAGACCTTGG TGGAAGAGAC CACAACAGAG
    TCCAGTGACG ATGAGGTGGT GGCACCTTCC CAGTCTCTCC TCTCAGGTTT TGTGACTCCT
    GGGCTGACTC CAGCTGGTTC CAAGACTTCA AAGGCCACTC CCAAACCAGA CACCAGCCCC
    TCTGTTTCCT CTACTCCGGC CACCAGAGAT GCCGCAGAAG GCAATCAGGA AGTGGAGCCC
    CAGCAAGCAG CAGGCACCAT GTCCCCTAAA ACAGGCAGAA AAGAGGCTGG TGCCACACCT
    CAGAAGCCCG GGAAGGGGCC TGGGAGTACC CCGGCCTCCA TGCTGGCGCT GCAGAGCAAC
    ATCACCCAGC GCCTCCTGAG CGAGCCCTGG CCCCTGAGTG AGGCGCAGGT GCAGGCCTCG
    GTGGCAAAAG TGCTCACGGA GCTGCTGGAG CAGGAGAGGA AGAAGGCCGT GGAGACCGCC
    AAGGAAGGCA ACCGGAAGGG CCGCCTGGGC CATAAGCGGA AGCTGTCCGA AGACCAGACA
    GCCCCCAAGG CCCCCAAGAG CAAGAAAAAG AAGCAGTTGG CAGCTGCAGA TAGTGGGGAG
    CATGTGGTCT CCTCAGAAAA GGCGCCCAGG ACTGTCAAAG GGAAATCCAA GAAGGACAGA
    GCAAGTGGTG ACGCCAAGGA GAAGAAGGAG AAGGAGTCTC CCGGCTCTCA GGGGGCCAAG
    GAGAAGCCAG TAGGGGAGCT GGGGACTCCG AAGAGTGACG GGGGAGACCA CGGCAACCTT
    AAGATCAAGA AAGAGAAGAA GAAATCTGAC AAGAGATGGG CAAAGCCAGA ACCAGGTCCT
    GGAGCAGCGG AGCCACCACC ACCAGGACGG CGCTCACAGC TCCAGGGCAT GTTCCGCTCG
    AAGAGCCCTG CCCTCCCCCA CGGCGGCCAC AGCCACGTGC ACTGGCAGCC GAGGCTCCTG
    GGGTCCAGGC TCCTGGATAG CAGCTGCCCC TTGCTGCATT CTGTGCTGGA GTCCTCGTGC
    AGCCGGGCCA CGTTTAACCC CCACCCCAGC CCTGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011968795.1
    CDS48..4526
    Translation

    Target ORF information:

    RefSeq Version XM_012113405.2
    Organism Ovis aries musimon(Ovis orientalis musimon)
    Definition Ovis aries musimon Treacher Collins-Franceschetti syndrome 1 (TCOF1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012113405.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGCTGAGG CCAGGAAGCG GCGGGAGCTC CTTCCCCTGA TCTACCAGCA TCTGCTGCAG 
    GCGGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA AATTTTCCTG
    AGTCAGCCTG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACTTC GGAGATTGGC
    CGGAAACGGA AGGCAGAGGA AGATGCGGCC TTGCAGGCCA AGAGGACCCG CGTGTCAGAC
    CCCATCAGCA GCTCAGAGAG CTCGGAAGAG GAGGAGGAGG AGGAGGAGGC AGAAGCCAAA
    ACCACCAAAC CCACCCCGAG ATTAGCAGCT ACCAACTCCT CAGTCACAGG GACAGTTTCG
    TCTTCAAATG TGAAAGAAAA AGCCAAGGCA AAGACCACGA AAGCCAGCAA GACGGTGAAC
    TCCACGACAC ACCCTGCCCC GGGAAAGGCA GTGGCCCACC TCCTCACTGG GAAGTCACCC
    CAGAAGACGA CGGGGCCCTC GGCAAATGCT GTCTTGGTGT CAGAAACTGA GGAGGAGGGC
    AGCGTGTCAG CTCTCAGAAC CGCTGCCACG CCTGGAATGG CATCTGCCGA CCAGGCTGAT
    AGCTCCAGTG AGGATACCAC CAGCTCGAGC GATGAGACGG ACTTGGAGGT GAAAGCCGCA
    GTAAAACCAC TGCAGGTCAG AGCCTCAGCC ACTGCAGTCA AGGAGTCTCC GAGAAAAAGT
    GCAGCCCCAA CTCCAGGGAA GGCAGGGGTT GTGACACCCC AAGTCAAAGG CGGTGCTGTA
    ACCCCAGCCA GCAGGGCCAA GAAGCCAGAA GAGGTCTCAG AGAGCAGCGA GGAGTCCGAT
    GAGAGTGAGG AGGAGGCCCC GGTGAAGGCC TTGGAGAAAA CCACCCCAAT CAGAGTTGCT
    CCTAGTCCTG CCAAGGGGAC CCCTGGGAAA GGGGCCACCC CAGCAGCCCC TGGGAAGGCA
    GGGCCCTTAG GGAAGCCAGA GGAGGACTCG GAGAGCAGCA GCGAGGAGTC AGACAGCGAG
    GAGGAGACAC AAGCTGTCAA GACCCCAGTG CAGGCAAAGC CCTCTGGGAA GATCCCCCAG
    GTTAAAGCTG CCTCTGCAGC ACCCACCCAG GTGCTTTCCC CTAAGAAAGG GGCCCCTCCA
    GCACCCGCTG GCAAGGCAGG GCCTGCAGCC ACCCAAGCCC AGCCCAGGAA GCAGGAGAAG
    GAGGAGAGCA GCAGCAGCAG CAGCGAGGAA TCAGACAGCG AGGGCGAGGC GCCCACGGCC
    ACGCGGCAGA CTACAAGCCT GGCTCAGGCA AAGCCCTTGG AGAAAAACTC TCAGGTCAGA
    GCTGCCTCAG CCACGGGTGC AGGACCCTCG GCGAAGGGCA CAGTCCCTGC GCCCCCTCAG
    AAGGCAAGGT CTGTGGCCGC CCAGGACGGG AAGCAGGAGG ACTCGGAGAG CAGCAGTGAG
    GAGGAGTCGG ACAGCGAGGA GGAGATGCAG AGGGCAGGGA CCTCAGCCCA GGCACAGTCC
    TCGGGGAAAG CCCTCCAGGT CAGACCTTCC TCAGGTCCCA CCAAGGGGCC CCCTCAGAAG
    GCCGGGCCTG CAACCACCCA GGTCAAGGTC GAAAGGGCCG AGGAAGACTC GGAGAGCAGT
    GAGGAGGAAT CAGACAGTGA GGACGAAGCA CCTGTAGCCA AGACTCCAGC CCAGGCAAAA
    TCAACTGTGA AAACTTCTCA GATCAAGGCC TCCCCAAGGA AGGGCACCCC CATTACACCC
    ACATCTGCCA GGGTCCCCCC AGTGCAAGTC GGCACACCAT CCCCCCGGAC AGCAGCAACA
    GTGAGTACGC CCACCTGTGC ATCATCCCCA GCCATAGTCA GGCACACCCA GAAGCCAGGG
    GAGGACTCTT CAAGCAGCGA GGAGTCTGAG AGCGAGGAGG AGACAGCTCC TGCTGCAACA
    GGGGGACAGA TGAAGTCTGT AGGGAAGAGC CTCCCAGTGA AAGCTGCCTC AGCATCCACC
    AAGGGGCCCT CAGGAAAAGG GACCACCCCA GGGCCCCCTG GGAAGGCAGG ACCCCCCGTG
    GCACAGGTCA AGGCTGAGGT GCAGGAAGAC TCAGAGAGCA GTGAGGAGTC GGACAGTGAG
    GAAGCGGCTG CAGCTCCCGC ACAGGTGAAG ACTTCAGTGA AAACCCCTCA GACCAAAGCC
    AACCCTTCTG CCACCAGAGT GGCTTCAGCC AAAGCAGCAG CACCAGCTCC TGGAAAGGGG
    GGCCTTGCTG TTGCTCAAGC CAAAGTGGGG TCCCCAGCCA AGGTAACCCC AGCAGCGAGA
    GCCCCCCAGA GCAGCGCCGT CTCTGGGAGC GGCCAGGTGT CCATGCCGGC TGTGGGGAAG
    GCAGCGGCTG CAGCAGCCCA GGCCCAGCCG GGGCCTGTCG GGGGCTCACA GGAGGACTCG
    GAGAGCAGTG AGGAGGAGTC GGACAGTGAG GGAGAGGCAC CTCCTCAGGC GAAGCCCTCA
    GGAAAGACCC CCCAGGTCAA AACTGCCTCA GCCCCCACCA AGGCGTGCCC CAAGAAAGGG
    GCTGCCCCAG TACCCCCTGG GAAGGCAGGA CCCCCAGCCG CCCAGGCCAG GAGGCAGGAG
    GAGGACTCGG AGAGCAGCAG CGAGGAGGAG TCAGACAGTG ACGGGGACGT GCCAGCAGCT
    GCACCTCCAG CCCAGAAGGA GGGTAGCTCC AAAACTGCCA CAAGCAAGAC CCTGACTCCA
    GCATCCCTGG AGAAGAAAGC AGAGGAGTCC TCAACCAGCA GTGATGAGGA CCTGCCGTCG
    AACCAGGTGA TTAAAACCCC TCTGATTTTT GTCGACCCTA ATCGTAGTCC AGCTAGCCCA
    GCTGCTACCC CCACCCAAGC GCAGGCTACA GGCACCCCAA GGAAGGCTCA GGCCTTGGAG
    AGCACAACCA GGAGCTCCTC TGAGAGCGAG GACGAGGACG TGATCCCCGC TACACAGTGC
    CCGACTCCTG CCACTAGGAC CAGCGTGGTG ACTGTGCCCA CAGCACCTCC AAAGGCGGCC
    CTCAGAGCCA GTGTGGTGGG GGCTCCCAGC AGTGAGGACA CAAGTCAGGG GTCTGAAGGC
    AAGAAGCAGG AGGCCTCAGC CACTCAGGTG ACCAAGAGGA ACCTCCCACT GACGCAGGCT
    GCCCTGAAGG TTCTTGCCCA GAAAGCCAGT GAGGCCCAGC CTCCTGCTGC CAAGACCCCG
    TCTTCAAGTG GGACTGACAG TGCTGTGGGA ACTCTCTCCA CGGTGAGTCC CCAGAGCACC
    CCTACCCAGG TCAGAATGGC CAACAAGCTC AGAAAGCCTG AGGTTCCTGC AGCTCAGCGG
    GCCACAGCTA CTCCCTCGGG GCACCCCACA CCCAAGGCTC CTGCAACCTC AGATGACAGC
    AATAGCAGCT CTTCGGGAAG CGAGGACGAA GCCAAGGGGT CCCAGGCGGC CCCGTCAGCC
    CACAGGCCAG GCTCAGCCCC CTCCAGGAGG GAGACCTTGG TGGAAGAGAC CACAACAGAG
    TCCAGTGACG ATGAGGTGGT GGCACCTTCC CAGTCTCTCC TCTCAGGTTT TGTGACTCCT
    GGGCTGACTC CAGCTGGTTC CAAGACTTCA AAGGCCACTC CCAAACCAGA CACCAGCCCC
    TCTGTTTCCT CTACTCCGGC CACCAGAGAT GCCGCAGAAG GCAATCAGGA AGTGGAGCCC
    CAGCAAGCAG CAGGCACCAT GTCCCCTAAA ACAGGCAGAA AAGAGGCTGG TGCCACACCT
    CAGAAGCCCG GGAAGGGGCC TGGGAGTACC CCGGCCTCCA TGCTGGCGCT GCAGAGCAAC
    ATCACCCAGC GCCTCCTGAG CGAGCCCTGG CCCCTGAGTG AGGCGCAGGT GCAGGCCTCG
    GTGGCAAAAG TGCTCACGGA GCTGCTGGAG CAGGAGAGGA AGAAGGCCGT GGAGACCGCC
    AAGGAAGGCA ACCGGAAGGG CCGCCTGGGC CATAAGCGGA AGCTGTCCGA AGACCAGACA
    GCCCCCAAGG CCCCCAAGAG CAAGAAAAAG AAGCAGTTGG CAGCTGCAGA TAGTGGGGAG
    CATGTGGTCT CCTCAGAAAA GGCGCCCAGG ACTGTCAAAG GGAAATCCAA GAAGGACAGA
    GCAAGTGGTG ACGCCAAGGA GAAGAAGGAG AAGGAGTCTC CCGGCTCTCA GGGGGCCAAG
    GAGAAGCCAG TAGGGGAGCT GGGGACTCCG AAGAGTGACG GGGGAGACCA CGGCAACCTT
    AAGATCAAGA AAGAGAAGAA GAAATCTGAC AAGAGATGGG CAAAGCCAGA ACCAGGTCCT
    GGAGCAGCGG AGCCACCACC ACCAGGACGG CGCTCACAGC TCCAGGGCAT GTTCCGCTCG
    AAGAGCCCTG CCCTCCCCCA CGGCGGCCAC AGCCACGTGC ACTGGCAGCC GAGGCTCCTG
    GGGTCCAGGC TCCTGGATAG CAGCTGCCCC TTGCTGCATT CTGTGCTGGA GTCCTCGTGC
    AGCCGGGCCA CGTTTAACCC CCACCCCAGC CCTGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OOv03149
    Clone ID Related Accession (Same CDS sequence) XM_012113406.2
    Accession Version XM_012113406.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4353bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-16
    Organism Ovis aries musimon(Ovis orientalis musimon)
    Product treacle protein isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942253.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012113406.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGCTGAGG CCAGGAAGCG GCGGGAGCTC CTTCCCCTGA TCTACCAGCA TCTGCTGCAG 
    GCGGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA AATTTTCCTG
    AGTCAGCCTG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACTTC GGAGATTGGC
    CGGAAACGGA AGGCAGAGGA AGATGCGGCC TTGCAGGCCA AGAGGACCCG CGTGTCAGAC
    CCCATCAGCA GCTCAGAGAG CTCGGAAGAG GAGGAGGAGG AGGAGGAGGC AGAAGCCAAA
    ACCACCAAAC CCACCCCGAG ATTAGCAGCT ACCAACTCCT CAGTCACAGG GACAGTTTCG
    TCTTCAAATG TGAAAGAAAA AGCCAAGGCA AAGACCACGA AAGCCAGCAA GACGGTGAAC
    TCCACGACAC ACCCTGCCCC GGGAAAGGCA GTGGCCCACC TCCTCACTGG GAAGTCACCC
    CAGAAGACGA CGGGGCCCTC GGCAAATGCT GTCTTGGTGT CAGAAACTGA GGAGGAGGGC
    AGCGTGTCAG CTCTCAGAAC CGCTGCCACG CCTGGAATGG CATCTGCCGA CCAGGCTGAT
    AGCTCCAGTG AGGATACCAC CAGCTCGAGC GATGAGACGG ACTTGGAGGT GAAAGCCGCA
    GTAAAACCAC TGCAGGTCAG AGCCTCAGCC ACTGCAGTCA AGGAGTCTCC GAGAAAAAGT
    GCAGCCCCAA CTCCAGGGAA GGCAGGGGTT GTGACACCCC AAGTCAAAGG CGGTGCTGTA
    ACCCCAGCCA GCAGGGCCAA GAAGCCAGAA GAGGTCTCAG AGAGCAGCGA GGAGTCCGAT
    GAGAGTGAGG AGGAGGCCCC GGTGAAGGCC TTGGAGAAAA CCACCCCAAT CAGAGTTGCT
    CCTAGTCCTG CCAAGGGGAC CCCTGGGAAA GGGGCCACCC CAGCAGCCCC TGGGAAGGCA
    GGGCCCTTAG GGAAGCCAGA GGAGGACTCG GAGAGCAGCA GCGAGGAGTC AGACAGCGAG
    GAGGAGACAC AAGCTGTCAA GACCCCAGTG CAGGTGGGAC CTGGAGAGAG GGCCCCGGAG
    CTGGCACCCA CCCCAGGAGG CCTCCTGAGG GCTGATTCTC CCACTCCACG TGCCCTGGCC
    ACGCAAGGGC CACGTGGAGT CTGTGGTGTC TTTGACCCCG TCTGCTATCC TGTTTCCTCA
    AGTCCACTGT CTGGGCTCTC TCCACTGATT CTGTTTCTCT CTCTTCAGGC AAAGCCCTCT
    GGGAAGATCC CCCAGGTTAA AGCTGCCTCT GCAGCACCCA CCCAGGTGCT TTCCCCTAAG
    AAAGGGGCCC CTCCAGCACC CGCTGGCAAG GCAGGGCCTG CAGCCACCCA AGCCCAGCCC
    AGGAAGCAGG AGAAGGAGGA GAGCAGCAGC AGCAGCAGCG AGGAATCAGA CAGCGAGGGC
    GAGGCGCCCA CGGCCACGCG GCAGACTACA AGCCTGGCTC AGGCAAAGCC CTTGGAGAAA
    AACTCTCAGG TCAGAGCTGC CTCAGCCACG GGTGCAGGAC CCTCGGCGAA GGGCACAGTC
    CCTGCGCCCC CTCAGAAGGC AAGGTCTGTG GCCGCCCAGG ACGGGAAGCA GGAGGACTCG
    GAGAGCAGCA GTGAGGAGGA GTCGGACAGC GAGGAGGAGA TGCAGAGGGC AGGGACCTCA
    GCCCAGGCAC AGTCCTCGGG GAAAGCCCTC CAGGTCAGAC CTTCCTCAGG TCCCACCAAG
    GGGCCCCCTC AGAAGGCCGG GCCTGCAACC ACCCAGGTCA AGGTCGAAAG GGCCGAGGAA
    GACTCGGAGA GCAGTGAGGA GGAATCAGAC AGTGAGGACG AAGCACCTGT AGCCAAGACT
    CCAGCCCAGG CAAAATCAAC TGTGAAAACT TCTCAGATCA AGGCCTCCCC AAGGAAGGGC
    ACCCCCATTA CACCCACATC TGCCAGGGTC CCCCCAGTGC AAGTCGGCAC ACCATCCCCC
    CGGACAGCAG CAACAGTGAG TACGCCCACC TGTGCATCAT CCCCAGCCAT AGTCAGGCAC
    ACCCAGAAGC CAGGGGAGGA CTCTTCAAGC AGCGAGGAGT CTGAGAGCGA GGAGGAGACA
    GCTCCTGCTG CAACAGGGGG ACAGATGAAG TCTGTAGGGA AGAGCCTCCC AGTGAAAGCT
    GCCTCAGCAT CCACCAAGGG GCCCTCAGGA AAAGGGACCA CCCCAGGGCC CCCTGGGAAG
    GCAGGACCCC CCGTGGCACA GGTCAAGGCT GAGGTGCAGG AAGACTCAGA GAGCAGTGAG
    GAGTCGGACA GTGAGGAAGC GGCTGCAGCT CCCGCACAGG TGAAGACTTC AGTGAAAACC
    CCTCAGACCA AAGCCAACCC TTCTGCCACC AGAGTGGCTT CAGCCAAAGC AGCAGCACCA
    GCTCCTGGAA AGGGGGGCCT TGCTGTTGCT CAAGCCAAAG TGGGGTCCCC AGCCAAGGTA
    ACCCCAGCAG CGAGAGCCCC CCAGAGCAGC GCCGTCTCTG GGAGCGGCCA GGTGTCCATG
    CCGGCTGTGG GGAAGGCAGC GGCTGCAGCA GCCCAGGCCC AGCCGGGGCC TGTCGGGGGC
    TCACAGGAGG ACTCGGAGAG CAGTGAGGAG GAGTCGGACA GTGAGGGAGA GGCACCTCCT
    CAGGCGAAGC CCTCAGGAAA GACCCCCCAG GTCAAAACTG CCTCAGCCCC CACCAAGGCG
    TGCCCCAAGA AAGGGGCTGC CCCAGTACCC CCTGGGAAGG CAGGACCCCC AGCCGCCCAG
    GCCAGGAGGC AGGAGGAGGA CTCGGAGAGC AGCAGCGAGG AGGAGTCAGA CAGTGACGGG
    GACGTGCCAG CAGCTGCACC TCCAGCCCAG AAGGAGGGTA GCTCCAAAAC TGCCACAAGC
    AAGACCCTGA CTCCAGCATC CCTGGAGAAG AAAGCAGAGG AGTCCTCAAC CAGCAGTGAT
    GAGGACCTGC CGTCGAACCA GGTGACCAAG AGGAACCTCC CACTGACGCA GGCTGCCCTG
    AAGGTTCTTG CCCAGAAAGC CAGTGAGGCC CAGCCTCCTG CTGCCAAGAC CCCGTCTTCA
    AGTGGGACTG ACAGTGCTGT GGGAACTCTC TCCACGGTGA GTCCCCAGAG CACCCCTACC
    CAGGTCAGAA TGGCCAACAA GCTCAGAAAG CCTGAGGTTC CTGCAGCTCA GCGGGCCACA
    GCTACTCCCT CGGGGCACCC CACACCCAAG GCTCCTGCAA CCTCAGATGA CAGCAATAGC
    AGCTCTTCGG GAAGCGAGGA CGAAGCCAAG GGGTCCCAGG CGGCCCCGTC AGCCCACAGG
    CCAGGCTCAG CCCCCTCCAG GAGGGAGACC TTGGTGGAAG AGACCACAAC AGAGTCCAGT
    GACGATGAGG TGGTGGCACC TTCCCAGTCT CTCCTCTCAG GTTTTGTGAC TCCTGGGCTG
    ACTCCAGCTG GTTCCAAGAC TTCAAAGGCC ACTCCCAAAC CAGACACCAG CCCCTCTGTT
    TCCTCTACTC CGGCCACCAG AGATGCCGCA GAAGGCAATC AGGAAGTGGA GCCCCAGCAA
    GCAGCAGGCA CCATGTCCCC TAAAACAGGC AGAAAAGAGG CTGGTGCCAC ACCTCAGAAG
    CCCGGGAAGG GGCCTGGGAG TACCCCGGCC TCCATGCTGG CGCTGCAGAG CAACATCACC
    CAGCGCCTCC TGAGCGAGCC CTGGCCCCTG AGTGAGGCGC AGGTGCAGGC CTCGGTGGCA
    AAAGTGCTCA CGGAGCTGCT GGAGCAGGAG AGGAAGAAGG CCGTGGAGAC CGCCAAGGAA
    GGCAACCGGA AGGGCCGCCT GGGCCATAAG CGGAAGCTGT CCGAAGACCA GACAGCCCCC
    AAGGCCCCCA AGAGCAAGAA AAAGAAGCAG TTGGCAGCTG CAGATAGTGG GGAGCATGTG
    GTCTCCTCAG AAAAGGCGCC CAGGACTGTC AAAGGGAAAT CCAAGAAGGA CAGAGCAAGT
    GGTGACGCCA AGGAGAAGAA GGAGAAGGAG TCTCCCGGCT CTCAGGGGGC CAAGGAGAAG
    CCAGTAGGGG AGCTGGGGAC TCCGAAGAGT GACGGGGGAG ACCACGGCAA CCTTAAGATC
    AAGAAAGAGA AGAAGAAATC TGACAAGAGA TGGGCAAAGC CAGAACCAGG TCCTGGAGCA
    GCGGAGCCAC CACCACCAGG ACGGCGCTCA CAGCTCCAGG GCATGTTCCG CTCGAAGAGC
    CCTGCCCTCC CCCACGGCGG CCACAGCCAC GTGCACTGGC AGCCGAGGCT CCTGGGGTCC
    AGGCTCCTGG ATAGCAGCTG CCCCTTGCTG CATTCTGTGC TGGAGTCCTC GTGCAGCCGG
    GCCACGTTTA ACCCCCACCC CAGCCCTGTC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011968796.1
    CDS48..4400
    Translation

    Target ORF information:

    RefSeq Version XM_012113406.2
    Organism Ovis aries musimon(Ovis orientalis musimon)
    Definition Ovis aries musimon Treacher Collins-Franceschetti syndrome 1 (TCOF1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012113406.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGCTGAGG CCAGGAAGCG GCGGGAGCTC CTTCCCCTGA TCTACCAGCA TCTGCTGCAG 
    GCGGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA AATTTTCCTG
    AGTCAGCCTG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACTTC GGAGATTGGC
    CGGAAACGGA AGGCAGAGGA AGATGCGGCC TTGCAGGCCA AGAGGACCCG CGTGTCAGAC
    CCCATCAGCA GCTCAGAGAG CTCGGAAGAG GAGGAGGAGG AGGAGGAGGC AGAAGCCAAA
    ACCACCAAAC CCACCCCGAG ATTAGCAGCT ACCAACTCCT CAGTCACAGG GACAGTTTCG
    TCTTCAAATG TGAAAGAAAA AGCCAAGGCA AAGACCACGA AAGCCAGCAA GACGGTGAAC
    TCCACGACAC ACCCTGCCCC GGGAAAGGCA GTGGCCCACC TCCTCACTGG GAAGTCACCC
    CAGAAGACGA CGGGGCCCTC GGCAAATGCT GTCTTGGTGT CAGAAACTGA GGAGGAGGGC
    AGCGTGTCAG CTCTCAGAAC CGCTGCCACG CCTGGAATGG CATCTGCCGA CCAGGCTGAT
    AGCTCCAGTG AGGATACCAC CAGCTCGAGC GATGAGACGG ACTTGGAGGT GAAAGCCGCA
    GTAAAACCAC TGCAGGTCAG AGCCTCAGCC ACTGCAGTCA AGGAGTCTCC GAGAAAAAGT
    GCAGCCCCAA CTCCAGGGAA GGCAGGGGTT GTGACACCCC AAGTCAAAGG CGGTGCTGTA
    ACCCCAGCCA GCAGGGCCAA GAAGCCAGAA GAGGTCTCAG AGAGCAGCGA GGAGTCCGAT
    GAGAGTGAGG AGGAGGCCCC GGTGAAGGCC TTGGAGAAAA CCACCCCAAT CAGAGTTGCT
    CCTAGTCCTG CCAAGGGGAC CCCTGGGAAA GGGGCCACCC CAGCAGCCCC TGGGAAGGCA
    GGGCCCTTAG GGAAGCCAGA GGAGGACTCG GAGAGCAGCA GCGAGGAGTC AGACAGCGAG
    GAGGAGACAC AAGCTGTCAA GACCCCAGTG CAGGTGGGAC CTGGAGAGAG GGCCCCGGAG
    CTGGCACCCA CCCCAGGAGG CCTCCTGAGG GCTGATTCTC CCACTCCACG TGCCCTGGCC
    ACGCAAGGGC CACGTGGAGT CTGTGGTGTC TTTGACCCCG TCTGCTATCC TGTTTCCTCA
    AGTCCACTGT CTGGGCTCTC TCCACTGATT CTGTTTCTCT CTCTTCAGGC AAAGCCCTCT
    GGGAAGATCC CCCAGGTTAA AGCTGCCTCT GCAGCACCCA CCCAGGTGCT TTCCCCTAAG
    AAAGGGGCCC CTCCAGCACC CGCTGGCAAG GCAGGGCCTG CAGCCACCCA AGCCCAGCCC
    AGGAAGCAGG AGAAGGAGGA GAGCAGCAGC AGCAGCAGCG AGGAATCAGA CAGCGAGGGC
    GAGGCGCCCA CGGCCACGCG GCAGACTACA AGCCTGGCTC AGGCAAAGCC CTTGGAGAAA
    AACTCTCAGG TCAGAGCTGC CTCAGCCACG GGTGCAGGAC CCTCGGCGAA GGGCACAGTC
    CCTGCGCCCC CTCAGAAGGC AAGGTCTGTG GCCGCCCAGG ACGGGAAGCA GGAGGACTCG
    GAGAGCAGCA GTGAGGAGGA GTCGGACAGC GAGGAGGAGA TGCAGAGGGC AGGGACCTCA
    GCCCAGGCAC AGTCCTCGGG GAAAGCCCTC CAGGTCAGAC CTTCCTCAGG TCCCACCAAG
    GGGCCCCCTC AGAAGGCCGG GCCTGCAACC ACCCAGGTCA AGGTCGAAAG GGCCGAGGAA
    GACTCGGAGA GCAGTGAGGA GGAATCAGAC AGTGAGGACG AAGCACCTGT AGCCAAGACT
    CCAGCCCAGG CAAAATCAAC TGTGAAAACT TCTCAGATCA AGGCCTCCCC AAGGAAGGGC
    ACCCCCATTA CACCCACATC TGCCAGGGTC CCCCCAGTGC AAGTCGGCAC ACCATCCCCC
    CGGACAGCAG CAACAGTGAG TACGCCCACC TGTGCATCAT CCCCAGCCAT AGTCAGGCAC
    ACCCAGAAGC CAGGGGAGGA CTCTTCAAGC AGCGAGGAGT CTGAGAGCGA GGAGGAGACA
    GCTCCTGCTG CAACAGGGGG ACAGATGAAG TCTGTAGGGA AGAGCCTCCC AGTGAAAGCT
    GCCTCAGCAT CCACCAAGGG GCCCTCAGGA AAAGGGACCA CCCCAGGGCC CCCTGGGAAG
    GCAGGACCCC CCGTGGCACA GGTCAAGGCT GAGGTGCAGG AAGACTCAGA GAGCAGTGAG
    GAGTCGGACA GTGAGGAAGC GGCTGCAGCT CCCGCACAGG TGAAGACTTC AGTGAAAACC
    CCTCAGACCA AAGCCAACCC TTCTGCCACC AGAGTGGCTT CAGCCAAAGC AGCAGCACCA
    GCTCCTGGAA AGGGGGGCCT TGCTGTTGCT CAAGCCAAAG TGGGGTCCCC AGCCAAGGTA
    ACCCCAGCAG CGAGAGCCCC CCAGAGCAGC GCCGTCTCTG GGAGCGGCCA GGTGTCCATG
    CCGGCTGTGG GGAAGGCAGC GGCTGCAGCA GCCCAGGCCC AGCCGGGGCC TGTCGGGGGC
    TCACAGGAGG ACTCGGAGAG CAGTGAGGAG GAGTCGGACA GTGAGGGAGA GGCACCTCCT
    CAGGCGAAGC CCTCAGGAAA GACCCCCCAG GTCAAAACTG CCTCAGCCCC CACCAAGGCG
    TGCCCCAAGA AAGGGGCTGC CCCAGTACCC CCTGGGAAGG CAGGACCCCC AGCCGCCCAG
    GCCAGGAGGC AGGAGGAGGA CTCGGAGAGC AGCAGCGAGG AGGAGTCAGA CAGTGACGGG
    GACGTGCCAG CAGCTGCACC TCCAGCCCAG AAGGAGGGTA GCTCCAAAAC TGCCACAAGC
    AAGACCCTGA CTCCAGCATC CCTGGAGAAG AAAGCAGAGG AGTCCTCAAC CAGCAGTGAT
    GAGGACCTGC CGTCGAACCA GGTGACCAAG AGGAACCTCC CACTGACGCA GGCTGCCCTG
    AAGGTTCTTG CCCAGAAAGC CAGTGAGGCC CAGCCTCCTG CTGCCAAGAC CCCGTCTTCA
    AGTGGGACTG ACAGTGCTGT GGGAACTCTC TCCACGGTGA GTCCCCAGAG CACCCCTACC
    CAGGTCAGAA TGGCCAACAA GCTCAGAAAG CCTGAGGTTC CTGCAGCTCA GCGGGCCACA
    GCTACTCCCT CGGGGCACCC CACACCCAAG GCTCCTGCAA CCTCAGATGA CAGCAATAGC
    AGCTCTTCGG GAAGCGAGGA CGAAGCCAAG GGGTCCCAGG CGGCCCCGTC AGCCCACAGG
    CCAGGCTCAG CCCCCTCCAG GAGGGAGACC TTGGTGGAAG AGACCACAAC AGAGTCCAGT
    GACGATGAGG TGGTGGCACC TTCCCAGTCT CTCCTCTCAG GTTTTGTGAC TCCTGGGCTG
    ACTCCAGCTG GTTCCAAGAC TTCAAAGGCC ACTCCCAAAC CAGACACCAG CCCCTCTGTT
    TCCTCTACTC CGGCCACCAG AGATGCCGCA GAAGGCAATC AGGAAGTGGA GCCCCAGCAA
    GCAGCAGGCA CCATGTCCCC TAAAACAGGC AGAAAAGAGG CTGGTGCCAC ACCTCAGAAG
    CCCGGGAAGG GGCCTGGGAG TACCCCGGCC TCCATGCTGG CGCTGCAGAG CAACATCACC
    CAGCGCCTCC TGAGCGAGCC CTGGCCCCTG AGTGAGGCGC AGGTGCAGGC CTCGGTGGCA
    AAAGTGCTCA CGGAGCTGCT GGAGCAGGAG AGGAAGAAGG CCGTGGAGAC CGCCAAGGAA
    GGCAACCGGA AGGGCCGCCT GGGCCATAAG CGGAAGCTGT CCGAAGACCA GACAGCCCCC
    AAGGCCCCCA AGAGCAAGAA AAAGAAGCAG TTGGCAGCTG CAGATAGTGG GGAGCATGTG
    GTCTCCTCAG AAAAGGCGCC CAGGACTGTC AAAGGGAAAT CCAAGAAGGA CAGAGCAAGT
    GGTGACGCCA AGGAGAAGAA GGAGAAGGAG TCTCCCGGCT CTCAGGGGGC CAAGGAGAAG
    CCAGTAGGGG AGCTGGGGAC TCCGAAGAGT GACGGGGGAG ACCACGGCAA CCTTAAGATC
    AAGAAAGAGA AGAAGAAATC TGACAAGAGA TGGGCAAAGC CAGAACCAGG TCCTGGAGCA
    GCGGAGCCAC CACCACCAGG ACGGCGCTCA CAGCTCCAGG GCATGTTCCG CTCGAAGAGC
    CCTGCCCTCC CCCACGGCGG CCACAGCCAC GTGCACTGGC AGCCGAGGCT CCTGGGGTCC
    AGGCTCCTGG ATAGCAGCTG CCCCTTGCTG CATTCTGTGC TGGAGTCCTC GTGCAGCCGG
    GCCACGTTTA ACCCCCACCC CAGCCCTGTC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OOv03150
    Clone ID Related Accession (Same CDS sequence) XM_012113409.2
    Accession Version XM_012113409.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4224bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-16
    Organism Ovis aries musimon(Ovis orientalis musimon)
    Product treacle protein isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942253.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012113409.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGCTGAGG CCAGGAAGCG GCGGGAGCTC CTTCCCCTGA TCTACCAGCA TCTGCTGCAG 
    GCGGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA AATTTTCCTG
    AGTCAGCCTG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACTTC GGAGATTGGC
    CGGAAACGGA AGGCAGAGGA AGATGCGGCC TTGCAGGCCA AGAGGACCCG CGTGTCAGAC
    CCCATCAGCA GCTCAGAGAG CTCGGAAGAG GAGGAGGAGG AGGAGGAGGC AGAAGCCAAA
    ACCACCAAAC CCACCCCGAG ATTAGCAGCT ACCAACTCCT CAGTCACAGG GACAGTTTCG
    TCTTCAAATG TGAAAGAAAA AGCCAAGGCA AAGACCACGA AAGCCAGCAA GACGGTGAAC
    TCCACGACAC ACCCTGCCCC GGGAAAGGCA GTGGCCCACC TCCTCACTGG GAAGTCACCC
    CAGAAGACGA CGGGGCCCTC GGCAAATGCT GTCTTGGTGT CAGAAACTGA GGAGGAGGGC
    AGCGTGTCAG CTCTCAGAAC CGCTGCCACG CCTGGAATGG CATCTGCCGA CCAGGCTGAT
    AGCTCCAGTG AGGATACCAC CAGCTCGAGC GATGAGACGG ACTTGGAGGT GAAAGCCGCA
    GTAAAACCAC TGCAGGTCAG AGCCTCAGCC ACTGCAGTCA AGGAGTCTCC GAGAAAAAGT
    GCAGCCCCAA CTCCAGGGAA GGCAGGGGTT GTGACACCCC AAGTCAAAGG CGGTGCTGTA
    ACCCCAGCCA GCAGGGCCAA GAAGCCAGAA GAGGTCTCAG AGAGCAGCGA GGAGTCCGAT
    GAGAGTGAGG AGGAGGCCCC GGTGAAGGCC TTGGAGAAAA CCACCCCAAT CAGAGTTGCT
    CCTAGTCCTG CCAAGGGGAC CCCTGGGAAA GGGGCCACCC CAGCAGCCCC TGGGAAGGCA
    GGGCCCTTAG GGAAGCCAGA GGAGGACTCG GAGAGCAGCA GCGAGGAGTC AGACAGCGAG
    GAGGAGACAC AAGCTGTCAA GACCCCAGTG CAGGTGGGAC CTGGAGAGAG GGCCCCGGAG
    CTGGCACCCA CCCCAGGAGG CCTCCTGAGG GCTGATTCTC CCACTCCACG TGCCCTGGCC
    ACGCAAGGGC CACGTGGAGT CTGTGGTGTC TTTGACCCCG TCTGCTATCC TGTTTCCTCA
    AGTCCACTGT CTGGGCTCTC TCCACTGATT CTGTTTCTCT CTCTTCAGGC AAAGCCCTCT
    GGGAAGATCC CCCAGGTTAA AGCTGCCTCT GCAGCACCCA CCCAGGTGCT TTCCCCTAAG
    AAAGGGGCCC CTCCAGCACC CGCTGGCAAG GCAGGGCCTG CAGCCACCCA AGCCCAGCCC
    AGGAAGCAGG AGAAGGAGGA GAGCAGCAGC AGCAGCAGCG AGGAATCAGA CAGCGAGGGC
    GAGGCGCCCA CGGCCACGCG GCAGACTACA AGCCTGGCTC AGGCAAAGCC CTTGGAGAAA
    AACTCTCAGG TCAGAGCTGC CTCAGCCACG GGTGCAGGAC CCTCGGCGAA GGGCACAGTC
    CCTGCGCCCC CTCAGAAGGC AAGGTCTGTG GCCGCCCAGG ACGGGAAGCA GGAGGACTCG
    GAGAGCAGCA GTGAGGAGGA GTCGGACAGC GAGGAGGAGA TGCAGAGGGC AGGGACCTCA
    GCCCAGGCAC AGTCCTCGGG GAAAGCCCTC CAGGTCAGAC CTTCCTCAGG TCCCACCAAG
    GGGCCCCCTC AGAAGGCCGG GCCTGCAACC ACCCAGGTCA AGGTCGAAAG GGCCGAGGAA
    GACTCGGAGA GCAGTGAGGA GGAATCAGAC AGTGAGGACG AAGCACCTGT AGCCAAGACT
    CCAGCCCAGG TGAAGACTTC AGTGAAAACC CCTCAGACCA AAGCCAACCC TTCTGCCACC
    AGAGTGGCTT CAGCCAAAGC AGCAGCACCA GCTCCTGGAA AGGGGGGCCT TGCTGTTGCT
    CAAGCCAAAG TGGGGTCCCC AGCCAAGGTA ACCCCAGCAG CGAGAGCCCC CCAGAGCAGC
    GCCGTCTCTG GGAGCGGCCA GGTGTCCATG CCGGCTGTGG GGAAGGCAGC GGCTGCAGCA
    GCCCAGGCCC AGCCGGGGCC TGTCGGGGGC TCACAGGAGG ACTCGGAGAG CAGTGAGGAG
    GAGTCGGACA GTGAGGGAGA GGCACCTCCT CAGGCGAAGC CCTCAGGAAA GACCCCCCAG
    GTCAAAACTG CCTCAGCCCC CACCAAGGCG TGCCCCAAGA AAGGGGCTGC CCCAGTACCC
    CCTGGGAAGG CAGGACCCCC AGCCGCCCAG GCCAGGAGGC AGGAGGAGGA CTCGGAGAGC
    AGCAGCGAGG AGGAGTCAGA CAGTGACGGG GACGTGCCAG CAGCTGCACC TCCAGCCCAG
    AAGGAGGGTA GCTCCAAAAC TGCCACAAGC AAGACCCTGA CTCCAGCATC CCTGGAGAAG
    AAAGCAGAGG AGTCCTCAAC CAGCAGTGAT GAGGACCTGC CGTCGAACCA GGTGATTAAA
    ACCCCTCTGA TTTTTGTCGA CCCTAATCGT AGTCCAGCTA GCCCAGCTGC TACCCCCACC
    CAAGCGCAGG CTACAGGCAC CCCAAGGAAG GCTCAGGCCT TGGAGAGCAC AACCAGGAGC
    TCCTCTGAGA GCGAGGACGA GGACGTGATC CCCGCTACAC AGTGCCCGAC TCCTGCCACT
    AGGACCAGCG TGGTGACTGT GCCCACAGCA CCTCCAAAGG CGGCCCTCAG AGCCAGTGTG
    GTGGGGGCTC CCAGCAGTGA GGACACAAGT CAGGGGTCTG AAGGCAAGAA GCAGGAGGCC
    TCAGCCACTC AGGTGACCAA GAGGAACCTC CCACTGACGC AGGCTGCCCT GAAGGTTCTT
    GCCCAGAAAG CCAGTGAGGC CCAGCCTCCT GCTGCCAAGA CCCCGTCTTC AAGTGGGACT
    GACAGTGCTG TGGGAACTCT CTCCACGGTG AGTCCCCAGA GCACCCCTAC CCAGGTCAGA
    ATGGCCAACA AGCTCAGAAA GCCTGAGGTT CCTGCAGCTC AGCGGGCCAC AGCTACTCCC
    TCGGGGCACC CCACACCCAA GGCTCCTGCA ACCTCAGATG ACAGCAATAG CAGCTCTTCG
    GGAAGCGAGG ACGAAGCCAA GGGGTCCCAG GCGGCCCCGT CAGCCCACAG GCCAGGCTCA
    GCCCCCTCCA GGAGGGAGAC CTTGGTGGAA GAGACCACAA CAGAGTCCAG TGACGATGAG
    GTGGTGGCAC CTTCCCAGTC TCTCCTCTCA GGTTTTGTGA CTCCTGGGCT GACTCCAGCT
    GGTTCCAAGA CTTCAAAGGC CACTCCCAAA CCAGACACCA GCCCCTCTGT TTCCTCTACT
    CCGGCCACCA GAGATGCCGC AGAAGGCAAT CAGGAAGTGG AGCCCCAGCA AGCAGCAGGC
    ACCATGTCCC CTAAAACAGG CAGAAAAGAG GCTGGTGCCA CACCTCAGAA GCCCGGGAAG
    GGGCCTGGGA GTACCCCGGC CTCCATGCTG GCGCTGCAGA GCAACATCAC CCAGCGCCTC
    CTGAGCGAGC CCTGGCCCCT GAGTGAGGCG CAGGTGCAGG CCTCGGTGGC AAAAGTGCTC
    ACGGAGCTGC TGGAGCAGGA GAGGAAGAAG GCCGTGGAGA CCGCCAAGGA AGGCAACCGG
    AAGGGCCGCC TGGGCCATAA GCGGAAGCTG TCCGAAGACC AGACAGCCCC CAAGGCCCCC
    AAGAGCAAGA AAAAGAAGCA GTTGGCAGCT GCAGATAGTG GGGAGCATGT GGTCTCCTCA
    GAAAAGGCGC CCAGGACTGT CAAAGGGAAA TCCAAGAAGG ACAGAGCAAG TGGTGACGCC
    AAGGAGAAGA AGGAGAAGGA GTCTCCCGGC TCTCAGGGGG CCAAGGAGAA GCCAGTAGGG
    GAGCTGGGGA CTCCGAAGAG TGACGGGGGA GACCACGGCA ACCTTAAGAT CAAGAAAGAG
    AAGAAGAAAT CTGACAAGAG ATGGGCAAAG CCAGAACCAG GTCCTGGAGC AGCGGAGCCA
    CCACCACCAG GACGGCGCTC ACAGCTCCAG GGCATGTTCC GCTCGAAGAG CCCTGCCCTC
    CCCCACGGCG GCCACAGCCA CGTGCACTGG CAGCCGAGGC TCCTGGGGTC CAGGCTCCTG
    GATAGCAGCT GCCCCTTGCT GCATTCTGTG CTGGAGTCCT CGTGCAGCCG GGCCACGTTT
    AACCCCCACC CCAGCCCTGT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011968799.1
    CDS48..4271
    Translation

    Target ORF information:

    RefSeq Version XM_012113409.2
    Organism Ovis aries musimon(Ovis orientalis musimon)
    Definition Ovis aries musimon Treacher Collins-Franceschetti syndrome 1 (TCOF1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012113409.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGCTGAGG CCAGGAAGCG GCGGGAGCTC CTTCCCCTGA TCTACCAGCA TCTGCTGCAG 
    GCGGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA AATTTTCCTG
    AGTCAGCCTG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACTTC GGAGATTGGC
    CGGAAACGGA AGGCAGAGGA AGATGCGGCC TTGCAGGCCA AGAGGACCCG CGTGTCAGAC
    CCCATCAGCA GCTCAGAGAG CTCGGAAGAG GAGGAGGAGG AGGAGGAGGC AGAAGCCAAA
    ACCACCAAAC CCACCCCGAG ATTAGCAGCT ACCAACTCCT CAGTCACAGG GACAGTTTCG
    TCTTCAAATG TGAAAGAAAA AGCCAAGGCA AAGACCACGA AAGCCAGCAA GACGGTGAAC
    TCCACGACAC ACCCTGCCCC GGGAAAGGCA GTGGCCCACC TCCTCACTGG GAAGTCACCC
    CAGAAGACGA CGGGGCCCTC GGCAAATGCT GTCTTGGTGT CAGAAACTGA GGAGGAGGGC
    AGCGTGTCAG CTCTCAGAAC CGCTGCCACG CCTGGAATGG CATCTGCCGA CCAGGCTGAT
    AGCTCCAGTG AGGATACCAC CAGCTCGAGC GATGAGACGG ACTTGGAGGT GAAAGCCGCA
    GTAAAACCAC TGCAGGTCAG AGCCTCAGCC ACTGCAGTCA AGGAGTCTCC GAGAAAAAGT
    GCAGCCCCAA CTCCAGGGAA GGCAGGGGTT GTGACACCCC AAGTCAAAGG CGGTGCTGTA
    ACCCCAGCCA GCAGGGCCAA GAAGCCAGAA GAGGTCTCAG AGAGCAGCGA GGAGTCCGAT
    GAGAGTGAGG AGGAGGCCCC GGTGAAGGCC TTGGAGAAAA CCACCCCAAT CAGAGTTGCT
    CCTAGTCCTG CCAAGGGGAC CCCTGGGAAA GGGGCCACCC CAGCAGCCCC TGGGAAGGCA
    GGGCCCTTAG GGAAGCCAGA GGAGGACTCG GAGAGCAGCA GCGAGGAGTC AGACAGCGAG
    GAGGAGACAC AAGCTGTCAA GACCCCAGTG CAGGTGGGAC CTGGAGAGAG GGCCCCGGAG
    CTGGCACCCA CCCCAGGAGG CCTCCTGAGG GCTGATTCTC CCACTCCACG TGCCCTGGCC
    ACGCAAGGGC CACGTGGAGT CTGTGGTGTC TTTGACCCCG TCTGCTATCC TGTTTCCTCA
    AGTCCACTGT CTGGGCTCTC TCCACTGATT CTGTTTCTCT CTCTTCAGGC AAAGCCCTCT
    GGGAAGATCC CCCAGGTTAA AGCTGCCTCT GCAGCACCCA CCCAGGTGCT TTCCCCTAAG
    AAAGGGGCCC CTCCAGCACC CGCTGGCAAG GCAGGGCCTG CAGCCACCCA AGCCCAGCCC
    AGGAAGCAGG AGAAGGAGGA GAGCAGCAGC AGCAGCAGCG AGGAATCAGA CAGCGAGGGC
    GAGGCGCCCA CGGCCACGCG GCAGACTACA AGCCTGGCTC AGGCAAAGCC CTTGGAGAAA
    AACTCTCAGG TCAGAGCTGC CTCAGCCACG GGTGCAGGAC CCTCGGCGAA GGGCACAGTC
    CCTGCGCCCC CTCAGAAGGC AAGGTCTGTG GCCGCCCAGG ACGGGAAGCA GGAGGACTCG
    GAGAGCAGCA GTGAGGAGGA GTCGGACAGC GAGGAGGAGA TGCAGAGGGC AGGGACCTCA
    GCCCAGGCAC AGTCCTCGGG GAAAGCCCTC CAGGTCAGAC CTTCCTCAGG TCCCACCAAG
    GGGCCCCCTC AGAAGGCCGG GCCTGCAACC ACCCAGGTCA AGGTCGAAAG GGCCGAGGAA
    GACTCGGAGA GCAGTGAGGA GGAATCAGAC AGTGAGGACG AAGCACCTGT AGCCAAGACT
    CCAGCCCAGG TGAAGACTTC AGTGAAAACC CCTCAGACCA AAGCCAACCC TTCTGCCACC
    AGAGTGGCTT CAGCCAAAGC AGCAGCACCA GCTCCTGGAA AGGGGGGCCT TGCTGTTGCT
    CAAGCCAAAG TGGGGTCCCC AGCCAAGGTA ACCCCAGCAG CGAGAGCCCC CCAGAGCAGC
    GCCGTCTCTG GGAGCGGCCA GGTGTCCATG CCGGCTGTGG GGAAGGCAGC GGCTGCAGCA
    GCCCAGGCCC AGCCGGGGCC TGTCGGGGGC TCACAGGAGG ACTCGGAGAG CAGTGAGGAG
    GAGTCGGACA GTGAGGGAGA GGCACCTCCT CAGGCGAAGC CCTCAGGAAA GACCCCCCAG
    GTCAAAACTG CCTCAGCCCC CACCAAGGCG TGCCCCAAGA AAGGGGCTGC CCCAGTACCC
    CCTGGGAAGG CAGGACCCCC AGCCGCCCAG GCCAGGAGGC AGGAGGAGGA CTCGGAGAGC
    AGCAGCGAGG AGGAGTCAGA CAGTGACGGG GACGTGCCAG CAGCTGCACC TCCAGCCCAG
    AAGGAGGGTA GCTCCAAAAC TGCCACAAGC AAGACCCTGA CTCCAGCATC CCTGGAGAAG
    AAAGCAGAGG AGTCCTCAAC CAGCAGTGAT GAGGACCTGC CGTCGAACCA GGTGATTAAA
    ACCCCTCTGA TTTTTGTCGA CCCTAATCGT AGTCCAGCTA GCCCAGCTGC TACCCCCACC
    CAAGCGCAGG CTACAGGCAC CCCAAGGAAG GCTCAGGCCT TGGAGAGCAC AACCAGGAGC
    TCCTCTGAGA GCGAGGACGA GGACGTGATC CCCGCTACAC AGTGCCCGAC TCCTGCCACT
    AGGACCAGCG TGGTGACTGT GCCCACAGCA CCTCCAAAGG CGGCCCTCAG AGCCAGTGTG
    GTGGGGGCTC CCAGCAGTGA GGACACAAGT CAGGGGTCTG AAGGCAAGAA GCAGGAGGCC
    TCAGCCACTC AGGTGACCAA GAGGAACCTC CCACTGACGC AGGCTGCCCT GAAGGTTCTT
    GCCCAGAAAG CCAGTGAGGC CCAGCCTCCT GCTGCCAAGA CCCCGTCTTC AAGTGGGACT
    GACAGTGCTG TGGGAACTCT CTCCACGGTG AGTCCCCAGA GCACCCCTAC CCAGGTCAGA
    ATGGCCAACA AGCTCAGAAA GCCTGAGGTT CCTGCAGCTC AGCGGGCCAC AGCTACTCCC
    TCGGGGCACC CCACACCCAA GGCTCCTGCA ACCTCAGATG ACAGCAATAG CAGCTCTTCG
    GGAAGCGAGG ACGAAGCCAA GGGGTCCCAG GCGGCCCCGT CAGCCCACAG GCCAGGCTCA
    GCCCCCTCCA GGAGGGAGAC CTTGGTGGAA GAGACCACAA CAGAGTCCAG TGACGATGAG
    GTGGTGGCAC CTTCCCAGTC TCTCCTCTCA GGTTTTGTGA CTCCTGGGCT GACTCCAGCT
    GGTTCCAAGA CTTCAAAGGC CACTCCCAAA CCAGACACCA GCCCCTCTGT TTCCTCTACT
    CCGGCCACCA GAGATGCCGC AGAAGGCAAT CAGGAAGTGG AGCCCCAGCA AGCAGCAGGC
    ACCATGTCCC CTAAAACAGG CAGAAAAGAG GCTGGTGCCA CACCTCAGAA GCCCGGGAAG
    GGGCCTGGGA GTACCCCGGC CTCCATGCTG GCGCTGCAGA GCAACATCAC CCAGCGCCTC
    CTGAGCGAGC CCTGGCCCCT GAGTGAGGCG CAGGTGCAGG CCTCGGTGGC AAAAGTGCTC
    ACGGAGCTGC TGGAGCAGGA GAGGAAGAAG GCCGTGGAGA CCGCCAAGGA AGGCAACCGG
    AAGGGCCGCC TGGGCCATAA GCGGAAGCTG TCCGAAGACC AGACAGCCCC CAAGGCCCCC
    AAGAGCAAGA AAAAGAAGCA GTTGGCAGCT GCAGATAGTG GGGAGCATGT GGTCTCCTCA
    GAAAAGGCGC CCAGGACTGT CAAAGGGAAA TCCAAGAAGG ACAGAGCAAG TGGTGACGCC
    AAGGAGAAGA AGGAGAAGGA GTCTCCCGGC TCTCAGGGGG CCAAGGAGAA GCCAGTAGGG
    GAGCTGGGGA CTCCGAAGAG TGACGGGGGA GACCACGGCA ACCTTAAGAT CAAGAAAGAG
    AAGAAGAAAT CTGACAAGAG ATGGGCAAAG CCAGAACCAG GTCCTGGAGC AGCGGAGCCA
    CCACCACCAG GACGGCGCTC ACAGCTCCAG GGCATGTTCC GCTCGAAGAG CCCTGCCCTC
    CCCCACGGCG GCCACAGCCA CGTGCACTGG CAGCCGAGGC TCCTGGGGTC CAGGCTCCTG
    GATAGCAGCT GCCCCTTGCT GCATTCTGTG CTGGAGTCCT CGTGCAGCCG GGCCACGTTT
    AACCCCCACC CCAGCCCTGT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OOv03151
    Clone ID Related Accession (Same CDS sequence) XM_012113410.2
    Accession Version XM_012113410.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4038bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-16
    Organism Ovis aries musimon(Ovis orientalis musimon)
    Product treacle protein isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942253.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012113410.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGCTGAGG CCAGGAAGCG GCGGGAGCTC CTTCCCCTGA TCTACCAGCA TCTGCTGCAG 
    GCGGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA AATTTTCCTG
    AGTCAGCCTG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACTTC GGAGATTGGC
    CGGAAACGGA AGGCAGAGGA AGATGCGGCC TTGCAGGCCA AGAGGACCCG CGTGTCAGAC
    CCCATCAGCA GCTCAGAGAG CTCGGAAGAG GAGGAGGAGG AGGAGGAGGC AGAAGCCAAA
    ACCACCAAAC CCACCCCGAG ATTAGCAGCT ACCAACTCCT CAGTCACAGG GACAGTTTCG
    TCTTCAAATG TGAAAGAAAA AGCCAAGGCA AAGACCACGA AAGCCAGCAA GACGGTGAAC
    TCCACGACAC ACCCTGCCCC GGGAAAGGCA GTGGCCCACC TCCTCACTGG GAAGTCACCC
    CAGAAGACGA CGGGGCCCTC GGCAAATGCT GTCTTGGTGT CAGAAACTGA GGAGGAGGGC
    AGCGTGTCAG CTCTCAGAAC CGCTGCCACG CCTGGAATGG CATCTGCCGA CCAGGCTGAT
    AGCTCCAGTG AGGATACCAC CAGCTCGAGC GATGAGACGG ACTTGGAGGT GAAAGCCGCA
    GTAAAACCAC TGCAGGTCAG AGCCTCAGCC ACTGCAGTCA AGGAGTCTCC GAGAAAAAGT
    GCAGCCCCAA CTCCAGGGAA GGCAGGGGTT GTGACACCCC AAGTCAAAGG CGGTGCTGTA
    ACCCCAGCCA GCAGGGCCAA GAAGCCAGAA GAGGTCTCAG AGAGCAGCGA GGAGTCCGAT
    GAGAGTGAGG AGGAGGCCCC GGTGAAGGCC TTGGAGAAAA CCACCCCAAT CAGAGTTGCT
    CCTAGTCCTG CCAAGGGGAC CCCTGGGAAA GGGGCCACCC CAGCAGCCCC TGGGAAGGCA
    GGGCCCTTAG GGAAGCCAGA GGAGGACTCG GAGAGCAGCA GCGAGGAGTC AGACAGCGAG
    GAGGAGACAC AAGCTGTCAA GACCCCAGTG CAGGCAAAGC CCTCTGGGAA GATCCCCCAG
    GTTAAAGCTG CCTCTGCAGC ACCCACCCAG GTGCTTTCCC CTAAGAAAGG GGCCCCTCCA
    GCACCCGCTG GCAAGGCAGG GCCTGCAGCC ACCCAAGCCC AGCCCAGGAA GCAGGAGAAG
    GAGGAGAGCA GCAGCAGCAG CAGCGAGGAA TCAGACAGCG AGGGCGAGGC GCCCACGGCC
    ACGCGGCAGA CTACAAGCCT GGCTCAGGCA AAGCCCTTGG AGAAAAACTC TCAGGTCAGA
    GCTGCCTCAG CCACGGGTGC AGGACCCTCG GCGAAGGGCA CAGTCCCTGC GCCCCCTCAG
    AAGGCAAGGT CTGTGGCCGC CCAGGACGGG AAGCAGGAGG ACTCGGAGAG CAGCAGTGAG
    GAGGAGTCGG ACAGCGAGGA GGAGATGCAG AGGGCAGGGA CCTCAGCCCA GGCACAGTCC
    TCGGGGAAAG CCCTCCAGGT CAGACCTTCC TCAGGTCCCA CCAAGGGGCC CCCTCAGAAG
    GCCGGGCCTG CAACCACCCA GGTCAAGGTC GAAAGGGCCG AGGAAGACTC GGAGAGCAGT
    GAGGAGGAAT CAGACAGTGA GGACGAAGCA CCTGTAGCCA AGACTCCAGC CCAGGCAAAA
    TCAACTGTGA AAACTTCTCA GATCAAGGCC TCCCCAAGGA AGGGCACCCC CATTACACCC
    ACATCTGCCA GGGTCCCCCC AGTGCAAGTC GGCACACCAT CCCCCCGGAC AGCAGCAACA
    GTGAGTACGC CCACCTGTGC ATCATCCCCA GCCATAGTCA GGCACACCCA GAAGCCAGGG
    GAGGACTCTT CAAGCAGCGA GGAGTCTGAG AGCGAGGAGG AGACAGCTCC TGCTGCAACA
    GGGGGACAGA TGAAGTCTGT AGGGAAGAGC CTCCCAGTGA AAGCTGCCTC AGCATCCACC
    AAGGGGCCCT CAGGAAAAGG GACCACCCCA GGGCCCCCTG GGAAGGCAGG ACCCCCCGTG
    GCACAGGTCA AGGCTGAGGT GCAGGAAGAC TCAGAGAGCA GTGAGGAGTC GGACAGTGAG
    GAAGCGGCTG CAGCTCCCGC ACAGGTGAAG ACTTCAGTGA AAACCCCTCA GACCAAAGCC
    AACCCTTCTG CCACCAGAGT GGCTTCAGCC AAAGCAGCAG CACCAGCTCC TGGAAAGGGG
    GGCCTTGCTG TTGCTCAAGC CAAAGTGGGG TCCCCAGCCA AGGTAACCCC AGCAGCGAGA
    GCCCCCCAGA GCAGCGCCGT CTCTGGGAGC GGCCAGGTGT CCATGCCGGC TGTGGGGAAG
    GCAGCGGCTG CAGCAGCCCA GGCCCAGCCG GGGCCTGTCG GGGGCTCACA GGAGGACTCG
    GAGAGCAGTG AGGAGGAGTC GGACAGTGAG GGAGAGGCAC CTCCTCAGGC GAAGCCCTCA
    GGAAAGACCC CCCAGGTCAA AACTGCCTCA GCCCCCACCA AGGCGTGCCC CAAGAAAGGG
    GCTGCCCCAG TACCCCCTGG GAAGGCAGGA CCCCCAGCCG CCCAGGCCAG GAGGCAGGAG
    GAGGACTCGG AGAGCAGCAG CGAGGAGGAG TCAGACAGTG ACGGGGACGT GCCAGCAGCT
    GCACCTCCAG CCCAGAAGGA GGGTAGCTCC AAAACTGCCA CAAGCAAGAC CCTGACTCCA
    GCATCCCTGG AGAAGAAAGC AGAGGAGTCC TCAACCAGCA GTGATGAGGA CCTGCCGTCG
    AACCAGGTGA CCAAGAGGAA CCTCCCACTG ACGCAGGCTG CCCTGAAGGT TCTTGCCCAG
    AAAGCCAGTG AGGCCCAGCC TCCTGCTGCC AAGACCCCGT CTTCAAGTGG GACTGACAGT
    GCTGTGGGAA CTCTCTCCAC GGTGAGTCCC CAGAGCACCC CTACCCAGGT CAGAATGGCC
    AACAAGCTCA GAAAGCCTGA GGTTCCTGCA GCTCAGCGGG CCACAGCTAC TCCCTCGGGG
    CACCCCACAC CCAAGGCTCC TGCAACCTCA GATGACAGCA ATAGCAGCTC TTCGGGAAGC
    GAGGACGAAG CCAAGGGGTC CCAGGCGGCC CCGTCAGCCC ACAGGCCAGG CTCAGCCCCC
    TCCAGGAGGG AGACCTTGGT GGAAGAGACC ACAACAGAGT CCAGTGACGA TGAGGTGGTG
    GCACCTTCCC AGTCTCTCCT CTCAGGTTTT GTGACTCCTG GGCTGACTCC AGCTGGTTCC
    AAGACTTCAA AGGCCACTCC CAAACCAGAC ACCAGCCCCT CTGTTTCCTC TACTCCGGCC
    ACCAGAGATG CCGCAGAAGG CAATCAGGAA GTGGAGCCCC AGCAAGCAGC AGGCACCATG
    TCCCCTAAAA CAGGCAGAAA AGAGGCTGGT GCCACACCTC AGAAGCCCGG GAAGGGGCCT
    GGGAGTACCC CGGCCTCCAT GCTGGCGCTG CAGAGCAACA TCACCCAGCG CCTCCTGAGC
    GAGCCCTGGC CCCTGAGTGA GGCGCAGGTG CAGGCCTCGG TGGCAAAAGT GCTCACGGAG
    CTGCTGGAGC AGGAGAGGAA GAAGGCCGTG GAGACCGCCA AGGAAGGCAA CCGGAAGGGC
    CGCCTGGGCC ATAAGCGGAA GCTGTCCGAA GACCAGACAG CCCCCAAGGC CCCCAAGAGC
    AAGAAAAAGA AGCAGTTGGC AGCTGCAGAT AGTGGGGAGC ATGTGGTCTC CTCAGAAAAG
    GCGCCCAGGA CTGTCAAAGG GAAATCCAAG AAGGACAGAG CAAGTGGTGA CGCCAAGGAG
    AAGAAGGAGA AGGAGTCTCC CGGCTCTCAG GGGGCCAAGG AGAAGCCAGT AGGGGAGCTG
    GGGACTCCGA AGAGTGACGG GGGAGACCAC GGCAACCTTA AGATCAAGAA AGAGAAGAAG
    AAATCTGACA AGAAAAAAAA AGACAAGGAG AAAAAGGAAA AGAAGAAGAA AACAAAAAAG
    GCCTCCACCA AAGACCCTGG CTCACCATCA CAGAAGAAAA AGAAGAGAAA GAAGAAGACA
    GCAGAGCAGA CTGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011968800.1
    CDS48..4085
    Translation

    Target ORF information:

    RefSeq Version XM_012113410.2
    Organism Ovis aries musimon(Ovis orientalis musimon)
    Definition Ovis aries musimon Treacher Collins-Franceschetti syndrome 1 (TCOF1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012113410.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGCTGAGG CCAGGAAGCG GCGGGAGCTC CTTCCCCTGA TCTACCAGCA TCTGCTGCAG 
    GCGGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA AATTTTCCTG
    AGTCAGCCTG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACTTC GGAGATTGGC
    CGGAAACGGA AGGCAGAGGA AGATGCGGCC TTGCAGGCCA AGAGGACCCG CGTGTCAGAC
    CCCATCAGCA GCTCAGAGAG CTCGGAAGAG GAGGAGGAGG AGGAGGAGGC AGAAGCCAAA
    ACCACCAAAC CCACCCCGAG ATTAGCAGCT ACCAACTCCT CAGTCACAGG GACAGTTTCG
    TCTTCAAATG TGAAAGAAAA AGCCAAGGCA AAGACCACGA AAGCCAGCAA GACGGTGAAC
    TCCACGACAC ACCCTGCCCC GGGAAAGGCA GTGGCCCACC TCCTCACTGG GAAGTCACCC
    CAGAAGACGA CGGGGCCCTC GGCAAATGCT GTCTTGGTGT CAGAAACTGA GGAGGAGGGC
    AGCGTGTCAG CTCTCAGAAC CGCTGCCACG CCTGGAATGG CATCTGCCGA CCAGGCTGAT
    AGCTCCAGTG AGGATACCAC CAGCTCGAGC GATGAGACGG ACTTGGAGGT GAAAGCCGCA
    GTAAAACCAC TGCAGGTCAG AGCCTCAGCC ACTGCAGTCA AGGAGTCTCC GAGAAAAAGT
    GCAGCCCCAA CTCCAGGGAA GGCAGGGGTT GTGACACCCC AAGTCAAAGG CGGTGCTGTA
    ACCCCAGCCA GCAGGGCCAA GAAGCCAGAA GAGGTCTCAG AGAGCAGCGA GGAGTCCGAT
    GAGAGTGAGG AGGAGGCCCC GGTGAAGGCC TTGGAGAAAA CCACCCCAAT CAGAGTTGCT
    CCTAGTCCTG CCAAGGGGAC CCCTGGGAAA GGGGCCACCC CAGCAGCCCC TGGGAAGGCA
    GGGCCCTTAG GGAAGCCAGA GGAGGACTCG GAGAGCAGCA GCGAGGAGTC AGACAGCGAG
    GAGGAGACAC AAGCTGTCAA GACCCCAGTG CAGGCAAAGC CCTCTGGGAA GATCCCCCAG
    GTTAAAGCTG CCTCTGCAGC ACCCACCCAG GTGCTTTCCC CTAAGAAAGG GGCCCCTCCA
    GCACCCGCTG GCAAGGCAGG GCCTGCAGCC ACCCAAGCCC AGCCCAGGAA GCAGGAGAAG
    GAGGAGAGCA GCAGCAGCAG CAGCGAGGAA TCAGACAGCG AGGGCGAGGC GCCCACGGCC
    ACGCGGCAGA CTACAAGCCT GGCTCAGGCA AAGCCCTTGG AGAAAAACTC TCAGGTCAGA
    GCTGCCTCAG CCACGGGTGC AGGACCCTCG GCGAAGGGCA CAGTCCCTGC GCCCCCTCAG
    AAGGCAAGGT CTGTGGCCGC CCAGGACGGG AAGCAGGAGG ACTCGGAGAG CAGCAGTGAG
    GAGGAGTCGG ACAGCGAGGA GGAGATGCAG AGGGCAGGGA CCTCAGCCCA GGCACAGTCC
    TCGGGGAAAG CCCTCCAGGT CAGACCTTCC TCAGGTCCCA CCAAGGGGCC CCCTCAGAAG
    GCCGGGCCTG CAACCACCCA GGTCAAGGTC GAAAGGGCCG AGGAAGACTC GGAGAGCAGT
    GAGGAGGAAT CAGACAGTGA GGACGAAGCA CCTGTAGCCA AGACTCCAGC CCAGGCAAAA
    TCAACTGTGA AAACTTCTCA GATCAAGGCC TCCCCAAGGA AGGGCACCCC CATTACACCC
    ACATCTGCCA GGGTCCCCCC AGTGCAAGTC GGCACACCAT CCCCCCGGAC AGCAGCAACA
    GTGAGTACGC CCACCTGTGC ATCATCCCCA GCCATAGTCA GGCACACCCA GAAGCCAGGG
    GAGGACTCTT CAAGCAGCGA GGAGTCTGAG AGCGAGGAGG AGACAGCTCC TGCTGCAACA
    GGGGGACAGA TGAAGTCTGT AGGGAAGAGC CTCCCAGTGA AAGCTGCCTC AGCATCCACC
    AAGGGGCCCT CAGGAAAAGG GACCACCCCA GGGCCCCCTG GGAAGGCAGG ACCCCCCGTG
    GCACAGGTCA AGGCTGAGGT GCAGGAAGAC TCAGAGAGCA GTGAGGAGTC GGACAGTGAG
    GAAGCGGCTG CAGCTCCCGC ACAGGTGAAG ACTTCAGTGA AAACCCCTCA GACCAAAGCC
    AACCCTTCTG CCACCAGAGT GGCTTCAGCC AAAGCAGCAG CACCAGCTCC TGGAAAGGGG
    GGCCTTGCTG TTGCTCAAGC CAAAGTGGGG TCCCCAGCCA AGGTAACCCC AGCAGCGAGA
    GCCCCCCAGA GCAGCGCCGT CTCTGGGAGC GGCCAGGTGT CCATGCCGGC TGTGGGGAAG
    GCAGCGGCTG CAGCAGCCCA GGCCCAGCCG GGGCCTGTCG GGGGCTCACA GGAGGACTCG
    GAGAGCAGTG AGGAGGAGTC GGACAGTGAG GGAGAGGCAC CTCCTCAGGC GAAGCCCTCA
    GGAAAGACCC CCCAGGTCAA AACTGCCTCA GCCCCCACCA AGGCGTGCCC CAAGAAAGGG
    GCTGCCCCAG TACCCCCTGG GAAGGCAGGA CCCCCAGCCG CCCAGGCCAG GAGGCAGGAG
    GAGGACTCGG AGAGCAGCAG CGAGGAGGAG TCAGACAGTG ACGGGGACGT GCCAGCAGCT
    GCACCTCCAG CCCAGAAGGA GGGTAGCTCC AAAACTGCCA CAAGCAAGAC CCTGACTCCA
    GCATCCCTGG AGAAGAAAGC AGAGGAGTCC TCAACCAGCA GTGATGAGGA CCTGCCGTCG
    AACCAGGTGA CCAAGAGGAA CCTCCCACTG ACGCAGGCTG CCCTGAAGGT TCTTGCCCAG
    AAAGCCAGTG AGGCCCAGCC TCCTGCTGCC AAGACCCCGT CTTCAAGTGG GACTGACAGT
    GCTGTGGGAA CTCTCTCCAC GGTGAGTCCC CAGAGCACCC CTACCCAGGT CAGAATGGCC
    AACAAGCTCA GAAAGCCTGA GGTTCCTGCA GCTCAGCGGG CCACAGCTAC TCCCTCGGGG
    CACCCCACAC CCAAGGCTCC TGCAACCTCA GATGACAGCA ATAGCAGCTC TTCGGGAAGC
    GAGGACGAAG CCAAGGGGTC CCAGGCGGCC CCGTCAGCCC ACAGGCCAGG CTCAGCCCCC
    TCCAGGAGGG AGACCTTGGT GGAAGAGACC ACAACAGAGT CCAGTGACGA TGAGGTGGTG
    GCACCTTCCC AGTCTCTCCT CTCAGGTTTT GTGACTCCTG GGCTGACTCC AGCTGGTTCC
    AAGACTTCAA AGGCCACTCC CAAACCAGAC ACCAGCCCCT CTGTTTCCTC TACTCCGGCC
    ACCAGAGATG CCGCAGAAGG CAATCAGGAA GTGGAGCCCC AGCAAGCAGC AGGCACCATG
    TCCCCTAAAA CAGGCAGAAA AGAGGCTGGT GCCACACCTC AGAAGCCCGG GAAGGGGCCT
    GGGAGTACCC CGGCCTCCAT GCTGGCGCTG CAGAGCAACA TCACCCAGCG CCTCCTGAGC
    GAGCCCTGGC CCCTGAGTGA GGCGCAGGTG CAGGCCTCGG TGGCAAAAGT GCTCACGGAG
    CTGCTGGAGC AGGAGAGGAA GAAGGCCGTG GAGACCGCCA AGGAAGGCAA CCGGAAGGGC
    CGCCTGGGCC ATAAGCGGAA GCTGTCCGAA GACCAGACAG CCCCCAAGGC CCCCAAGAGC
    AAGAAAAAGA AGCAGTTGGC AGCTGCAGAT AGTGGGGAGC ATGTGGTCTC CTCAGAAAAG
    GCGCCCAGGA CTGTCAAAGG GAAATCCAAG AAGGACAGAG CAAGTGGTGA CGCCAAGGAG
    AAGAAGGAGA AGGAGTCTCC CGGCTCTCAG GGGGCCAAGG AGAAGCCAGT AGGGGAGCTG
    GGGACTCCGA AGAGTGACGG GGGAGACCAC GGCAACCTTA AGATCAAGAA AGAGAAGAAG
    AAATCTGACA AGAAAAAAAA AGACAAGGAG AAAAAGGAAA AGAAGAAGAA AACAAAAAAG
    GCCTCCACCA AAGACCCTGG CTCACCATCA CAGAAGAAAA AGAAGAGAAA GAAGAAGACA
    GCAGAGCAGA CTGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OOv03152
    Clone ID Related Accession (Same CDS sequence) XM_012113411.2
    Accession Version XM_012113411.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3717bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-16
    Organism Ovis aries musimon(Ovis orientalis musimon)
    Product treacle protein isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942253.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012113411.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCTGAGG CCAGGAAGCG GCGGGAGCTC CTTCCCCTGA TCTACCAGCA TCTGCTGCAG 
    GCGGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA AATTTTCCTG
    AGTCAGCCTG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACTTC GGAGATTGGC
    CGGAAACGGA AGGCAGAGGA AGATGCGGCC TTGCAGGCCA AGAGGACCCG CGTGTCAGAC
    CCCATCAGCA GCTCAGAGAG CTCGGAAGAG GAGGAGGAGG AGGAGGAGGC AGAAGCCAAA
    ACCACCAAAC CCACCCCGAG ATTAGCAGCT ACCAACTCCT CAGTCACAGG GACAGTTTCG
    TCTTCAAATG TGAAAGAAAA AGCCAAGGCA AAGACCACGA AAGCCAGCAA GACGGTGAAC
    TCCACGACAC ACCCTGCCCC GGGAAAGGCA GTGGCCCACC TCCTCACTGG GAAGTCACCC
    CAGAAGACGA CGGGGCCCTC GGCAAATGCT GTCTTGGTGT CAGAAACTGA GGAGGAGGGC
    AGCGTGTCAG CTCTCAGAAC CGCTGCCACG CCTGGAATGG CATCTGCCGA CCAGGCTGAT
    AGCTCCAGTG AGGATACCAC CAGCTCGAGC GATGAGACGG ACTTGGAGGT GAAAGCCGCA
    GTAAAACCAC TGCAGGTCAG AGCCTCAGCC ACTGCAGTCA AGGAGTCTCC GAGAAAAAGT
    GCAGCCCCAA CTCCAGGGAA GGCAGGGGTT GTGACACCCC AAGTCAAAGG CGGTGCTGTA
    ACCCCAGCCA GCAGGGCCAA GAAGCCAGAA GAGGTCTCAG AGAGCAGCGA GGAGTCCGAT
    GAGAGTGAGG AGGAGGCCCC GGTGAAGGCC TTGGAGAAAA CCACCCCAAT CAGAGTTGCT
    CCTAGTCCTG CCAAGGGGAC CCCTGGGAAA GGGGCCACCC CAGCAGCCCC TGGGAAGGCA
    GGGCCCTTAG GGAAGCCAGA GGAGGACTCG GAGAGCAGCA GCGAGGAGTC AGACAGCGAG
    GAGGAGACAC AAGCTGTCAA GACCCCAGTG CAGGTGGGAC CTGGAGAGAG GGCCCCGGAG
    CTGGCACCCA CCCCAGGAGG CCTCCTGAGG GCTGATTCTC CCACTCCACG TGCCCTGGCC
    ACGCAAGGGC CACGTGGAGT CTGTGGTGTC TTTGACCCCG TCTGCTATCC TGTTTCCTCA
    AGTCCACTGT CTGGGCTCTC TCCACTGATT CTGTTTCTCT CTCTTCAGGC AAAGCCCTCT
    GGGAAGATCC CCCAGGTTAA AGCTGCCTCT GCAGCACCCA CCCAGGTGCT TTCCCCTAAG
    AAAGGGGCCC CTCCAGCACC CGCTGGCAAG GCAGGGCCTG CAGCCACCCA AGCCCAGCCC
    AGGAAGCAGG AGAAGGAGGA GAGCAGCAGC AGCAGCAGCG AGGAATCAGA CAGCGAGGGC
    GAGGCGCCCA CGGCCACGCG GCAGACTACA AGCCTGGCTC AGGCAAAGCC CTTGGAGAAA
    AACTCTCAGG TCAGAGCTGC CTCAGCCACG GGTGCAGGAC CCTCGGCGAA GGGCACAGTC
    CCTGCGCCCC CTCAGAAGGC AAGGTCTGTG GCCGCCCAGG ACGGGAAGCA GGAGGACTCG
    GAGAGCAGCA GTGAGGAGGA GTCGGACAGC GAGGAGGAGA TGCAGAGGGC AGGGACCTCA
    GCCCAGGCAC AGTCCTCGGG GAAAGCCCTC CAGGTCAGAC CTTCCTCAGG TCCCACCAAG
    GGGCCCCCTC AGAAGGCCGG GCCTGCAACC ACCCAGGTCA AGGTCGAAAG GGCCGAGGAA
    GACTCGGAGA GCAGTGAGGA GGAATCAGAC AGTGAGGACG AAGCACCTGT AGCCAAGACT
    CCAGCCCAGG CAAAATCAAC TGTGAAAACT TCTCAGATCA AGGCCTCCCC AAGGAAGGGC
    ACCCCCATTA CACCCACATC TGCCAGGGTC CCCCCAGTGC AAGTCGGCAC ACCATCCCCC
    CGGACAGCAG CAACAGTGAG TACGCCCACC TGTGCATCAT CCCCAGCCAT AGTCAGGCAC
    ACCCAGAAGC CAGGGGAGGA CTCTTCAAGC AGCGAGGAGT CTGAGAGCGA GGAGGAGACA
    GCTCCTGCTG CAACAGGGGG ACAGATGAAG TCTGTAGGGA AGAGCCTCCC AGTGAAAGCT
    GCCTCAGCAT CCACCAAGGG GCCCTCAGGA AAAGGGACCA CCCCAGGGCC CCCTGGGAAG
    GCAGGACCCC CCGTGGCACA GGTCAAGGCT GAGGTGCAGG AAGACTCAGA GAGCAGTGAG
    GAGTCGGACA GTGAGGAAGC GGCTGCAGCT CCCGCACAGG TGAAGACTTC AGTGAAAACC
    CCTCAGACCA AAGCCAACCC TTCTGCCACC AGAGTGGCTT CAGCCAAAGC AGCAGCACCA
    GCTCCTGGAA AGGGGGGCCT TGCTGTTGCT CAAGCCAAAG TGGGGTCCCC AGCCAAGGTA
    ACCCCAGCAG CGAGAGCCCC CCAGAGCAGC GCCGTCTCTG GGAGCGGCCA GGTGTCCATG
    CCGGCTGTGG GGAAGGCAGC GGCTGCAGCA GCCCAGGCCC AGCCGGGGCC TGTCGGGGGC
    TCACAGGAGG ACTCGGAGAG CAGTGAGGAG GAGTCGGACA GTGAGGGAGA GGCACCTCCT
    CAGGCGAAGC CCTCAGGAAA GACCCCCCAG GTCAAAACTG CCTCAGCCCC CACCAAGGCG
    TGCCCCAAGA AAGGGGCTGC CCCAGTACCC CCTGGGAAGG CAGGACCCCC AGCCGCCCAG
    GCCAGGAGGC AGGAGGAGGA CTCGGAGAGC AGCAGCGAGG AGGAGTCAGA CAGTGACGGG
    GACGTGCCAG CAGCTGCACC TCCAGCCCAG AAGGAGGGTA GCTCCAAAAC TGCCACAAGC
    AAGACCCTGA CTCCAGCATC CCTGGAGAAG AAAGCAGAGG AGTCCTCAAC CAGCAGTGAT
    GAGGACCTGC CGTCGAACCA GGTGATTAAA ACCCCTCTGA TTTTTGTCGA CCCTAATCGT
    AGTCCAGCTA GCCCAGCTGC TACCCCCACC CAAGCGCAGG CTACAGGCAC CCCAAGGAAG
    GCTCAGGCCT TGGAGAGCAC AACCAGGAGC TCCTCTGAGA GCGAGGACGA GGACGTGATC
    CCCGCTACAC AGTGCCCGAC TCCTGCCACT AGGACCAGCG TGGTGACTGT GCCCACAGCA
    CCTCCAAAGG CGGCCCTCAG AGCCAGTGTG GTGGGGGCTC CCAGCAGTGA GGACACAAGT
    CAGGGGTCTG AAGGCAAGAA GCAGGAGGCC TCAGCCACTC AGGTGACCAA GAGGAACCTC
    CCACTGACGC AGGCTGCCCT GAAGGTTCTT GCCCAGAAAG CCAGTGAGGC CCAGCCTCCT
    GCTGCCAAGA CCCCGTCTTC AAGTGGGACT GACAGTGCTG TGGGAACTCT CTCCACGGTG
    AGTCCCCAGA GCACCCCTAC CCAGGTCAGA ATGGCCAACA AGCTCAGAAA GCCTGAGGTT
    CCTGCAGCTC AGCGGGCCAC AGCTACTCCC TCGGGGCACC CCACACCCAA GGCTCCTGCA
    ACCTCAGATG ACAGCAATAG CAGCTCTTCG GGAAGCGAGG ACGAAGCCAA GGGGTCCCAG
    GCGGCCCCGT CAGCCCACAG GCCAGGCTCA GCCCCCTCCA GGAGGGAGAC CTTGGTGGAA
    GAGACCACAA CAGAGTCCAG TGACGATGAG GTGGTGGCAC CTTCCCAGGT TTTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011968801.1
    CDS48..3764
    Translation

    Target ORF information:

    RefSeq Version XM_012113411.2
    Organism Ovis aries musimon(Ovis orientalis musimon)
    Definition Ovis aries musimon Treacher Collins-Franceschetti syndrome 1 (TCOF1), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012113411.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCTGAGG CCAGGAAGCG GCGGGAGCTC CTTCCCCTGA TCTACCAGCA TCTGCTGCAG 
    GCGGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA AATTTTCCTG
    AGTCAGCCTG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACTTC GGAGATTGGC
    CGGAAACGGA AGGCAGAGGA AGATGCGGCC TTGCAGGCCA AGAGGACCCG CGTGTCAGAC
    CCCATCAGCA GCTCAGAGAG CTCGGAAGAG GAGGAGGAGG AGGAGGAGGC AGAAGCCAAA
    ACCACCAAAC CCACCCCGAG ATTAGCAGCT ACCAACTCCT CAGTCACAGG GACAGTTTCG
    TCTTCAAATG TGAAAGAAAA AGCCAAGGCA AAGACCACGA AAGCCAGCAA GACGGTGAAC
    TCCACGACAC ACCCTGCCCC GGGAAAGGCA GTGGCCCACC TCCTCACTGG GAAGTCACCC
    CAGAAGACGA CGGGGCCCTC GGCAAATGCT GTCTTGGTGT CAGAAACTGA GGAGGAGGGC
    AGCGTGTCAG CTCTCAGAAC CGCTGCCACG CCTGGAATGG CATCTGCCGA CCAGGCTGAT
    AGCTCCAGTG AGGATACCAC CAGCTCGAGC GATGAGACGG ACTTGGAGGT GAAAGCCGCA
    GTAAAACCAC TGCAGGTCAG AGCCTCAGCC ACTGCAGTCA AGGAGTCTCC GAGAAAAAGT
    GCAGCCCCAA CTCCAGGGAA GGCAGGGGTT GTGACACCCC AAGTCAAAGG CGGTGCTGTA
    ACCCCAGCCA GCAGGGCCAA GAAGCCAGAA GAGGTCTCAG AGAGCAGCGA GGAGTCCGAT
    GAGAGTGAGG AGGAGGCCCC GGTGAAGGCC TTGGAGAAAA CCACCCCAAT CAGAGTTGCT
    CCTAGTCCTG CCAAGGGGAC CCCTGGGAAA GGGGCCACCC CAGCAGCCCC TGGGAAGGCA
    GGGCCCTTAG GGAAGCCAGA GGAGGACTCG GAGAGCAGCA GCGAGGAGTC AGACAGCGAG
    GAGGAGACAC AAGCTGTCAA GACCCCAGTG CAGGTGGGAC CTGGAGAGAG GGCCCCGGAG
    CTGGCACCCA CCCCAGGAGG CCTCCTGAGG GCTGATTCTC CCACTCCACG TGCCCTGGCC
    ACGCAAGGGC CACGTGGAGT CTGTGGTGTC TTTGACCCCG TCTGCTATCC TGTTTCCTCA
    AGTCCACTGT CTGGGCTCTC TCCACTGATT CTGTTTCTCT CTCTTCAGGC AAAGCCCTCT
    GGGAAGATCC CCCAGGTTAA AGCTGCCTCT GCAGCACCCA CCCAGGTGCT TTCCCCTAAG
    AAAGGGGCCC CTCCAGCACC CGCTGGCAAG GCAGGGCCTG CAGCCACCCA AGCCCAGCCC
    AGGAAGCAGG AGAAGGAGGA GAGCAGCAGC AGCAGCAGCG AGGAATCAGA CAGCGAGGGC
    GAGGCGCCCA CGGCCACGCG GCAGACTACA AGCCTGGCTC AGGCAAAGCC CTTGGAGAAA
    AACTCTCAGG TCAGAGCTGC CTCAGCCACG GGTGCAGGAC CCTCGGCGAA GGGCACAGTC
    CCTGCGCCCC CTCAGAAGGC AAGGTCTGTG GCCGCCCAGG ACGGGAAGCA GGAGGACTCG
    GAGAGCAGCA GTGAGGAGGA GTCGGACAGC GAGGAGGAGA TGCAGAGGGC AGGGACCTCA
    GCCCAGGCAC AGTCCTCGGG GAAAGCCCTC CAGGTCAGAC CTTCCTCAGG TCCCACCAAG
    GGGCCCCCTC AGAAGGCCGG GCCTGCAACC ACCCAGGTCA AGGTCGAAAG GGCCGAGGAA
    GACTCGGAGA GCAGTGAGGA GGAATCAGAC AGTGAGGACG AAGCACCTGT AGCCAAGACT
    CCAGCCCAGG CAAAATCAAC TGTGAAAACT TCTCAGATCA AGGCCTCCCC AAGGAAGGGC
    ACCCCCATTA CACCCACATC TGCCAGGGTC CCCCCAGTGC AAGTCGGCAC ACCATCCCCC
    CGGACAGCAG CAACAGTGAG TACGCCCACC TGTGCATCAT CCCCAGCCAT AGTCAGGCAC
    ACCCAGAAGC CAGGGGAGGA CTCTTCAAGC AGCGAGGAGT CTGAGAGCGA GGAGGAGACA
    GCTCCTGCTG CAACAGGGGG ACAGATGAAG TCTGTAGGGA AGAGCCTCCC AGTGAAAGCT
    GCCTCAGCAT CCACCAAGGG GCCCTCAGGA AAAGGGACCA CCCCAGGGCC CCCTGGGAAG
    GCAGGACCCC CCGTGGCACA GGTCAAGGCT GAGGTGCAGG AAGACTCAGA GAGCAGTGAG
    GAGTCGGACA GTGAGGAAGC GGCTGCAGCT CCCGCACAGG TGAAGACTTC AGTGAAAACC
    CCTCAGACCA AAGCCAACCC TTCTGCCACC AGAGTGGCTT CAGCCAAAGC AGCAGCACCA
    GCTCCTGGAA AGGGGGGCCT TGCTGTTGCT CAAGCCAAAG TGGGGTCCCC AGCCAAGGTA
    ACCCCAGCAG CGAGAGCCCC CCAGAGCAGC GCCGTCTCTG GGAGCGGCCA GGTGTCCATG
    CCGGCTGTGG GGAAGGCAGC GGCTGCAGCA GCCCAGGCCC AGCCGGGGCC TGTCGGGGGC
    TCACAGGAGG ACTCGGAGAG CAGTGAGGAG GAGTCGGACA GTGAGGGAGA GGCACCTCCT
    CAGGCGAAGC CCTCAGGAAA GACCCCCCAG GTCAAAACTG CCTCAGCCCC CACCAAGGCG
    TGCCCCAAGA AAGGGGCTGC CCCAGTACCC CCTGGGAAGG CAGGACCCCC AGCCGCCCAG
    GCCAGGAGGC AGGAGGAGGA CTCGGAGAGC AGCAGCGAGG AGGAGTCAGA CAGTGACGGG
    GACGTGCCAG CAGCTGCACC TCCAGCCCAG AAGGAGGGTA GCTCCAAAAC TGCCACAAGC
    AAGACCCTGA CTCCAGCATC CCTGGAGAAG AAAGCAGAGG AGTCCTCAAC CAGCAGTGAT
    GAGGACCTGC CGTCGAACCA GGTGATTAAA ACCCCTCTGA TTTTTGTCGA CCCTAATCGT
    AGTCCAGCTA GCCCAGCTGC TACCCCCACC CAAGCGCAGG CTACAGGCAC CCCAAGGAAG
    GCTCAGGCCT TGGAGAGCAC AACCAGGAGC TCCTCTGAGA GCGAGGACGA GGACGTGATC
    CCCGCTACAC AGTGCCCGAC TCCTGCCACT AGGACCAGCG TGGTGACTGT GCCCACAGCA
    CCTCCAAAGG CGGCCCTCAG AGCCAGTGTG GTGGGGGCTC CCAGCAGTGA GGACACAAGT
    CAGGGGTCTG AAGGCAAGAA GCAGGAGGCC TCAGCCACTC AGGTGACCAA GAGGAACCTC
    CCACTGACGC AGGCTGCCCT GAAGGTTCTT GCCCAGAAAG CCAGTGAGGC CCAGCCTCCT
    GCTGCCAAGA CCCCGTCTTC AAGTGGGACT GACAGTGCTG TGGGAACTCT CTCCACGGTG
    AGTCCCCAGA GCACCCCTAC CCAGGTCAGA ATGGCCAACA AGCTCAGAAA GCCTGAGGTT
    CCTGCAGCTC AGCGGGCCAC AGCTACTCCC TCGGGGCACC CCACACCCAA GGCTCCTGCA
    ACCTCAGATG ACAGCAATAG CAGCTCTTCG GGAAGCGAGG ACGAAGCCAA GGGGTCCCAG
    GCGGCCCCGT CAGCCCACAG GCCAGGCTCA GCCCCCTCCA GGAGGGAGAC CTTGGTGGAA
    GAGACCACAA CAGAGTCCAG TGACGATGAG GTGGTGGCAC CTTCCCAGGT TTTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OOv03153
    Clone ID Related Accession (Same CDS sequence) XM_012113412.2
    Accession Version XM_012113412.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4497bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-16
    Organism Ovis aries musimon(Ovis orientalis musimon)
    Product treacle protein isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942253.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012113412.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGCTGAGG CCAGGAAGCG GCGGGAGCTC CTTCCCCTGA TCTACCAGCA TCTGCTGCAG 
    GCGGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA AATTTTCCTG
    AGTCAGCCTG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACTTC GGAGATTGGC
    CGGAAACGGA AGGCAGAGGA AGATGCGGCC TTGCAGGCCA AGAGGACCCG CGTGTCAGAC
    CCCATCAGCA GCTCAGAGAG CTCGGAAGAG GAGGAGGAGG AGGAGGAGGC AGAAGCCAAA
    ACCACCAAAC CCACCCCGAG ATTAGCAGCT ACCAACTCCT CAGTCACAGG GACAGTTTCG
    TCTTCAAATG TGAAAGAAAA AGCCAAGGCA AAGACCACGA AAGCCAGCAA GACGGTGAAC
    TCCACGACAC ACCCTGCCCC GGGAAAGGCA GTGGCCCACC TCCTCACTGG GAAGTCACCC
    CAGAAGACGA CGGGGCCCTC GGCAAATGCT GTCTTGGTGT CAGAAACTGA GGAGGAGGGC
    AGCGTGTCAG CTCTCAGAAC CGCTGCCACG CCTGGAATGG CATCTGCCGA CCAGGCTGAT
    AGCTCCAGTG AGGATACCAC CAGCTCGAGC GATGAGACGG ACTTGGAGGT GAAAGCCGCA
    GTAAAACCAC TGCAGGTCAG AGCCTCAGCC ACTGCAGTCA AGGAGTCTCC GAGAAAAAGT
    GCAGCCCCAA CTCCAGGGAA GGCAGGGGTT GTGACACCCC AAGTCAAAGG CGGTGCTGTA
    ACCCCAGCCA GCAGGGCCAA GAAGCCAGAA GAGGTCTCAG AGAGCAGCGA GGAGTCCGAT
    GAGAGTGAGG AGGAGGCCCC GGTGAAGGCC TTGGAGAAAA CCACCCCAAT CAGAGTTGCT
    CCTAGTCCTG CCAAGGGGAC CCCTGGGAAA GGGGCCACCC CAGCAGCCCC TGGGAAGGCA
    GGGCCCTTAG GGAAGCCAGA GGAGGACTCG GAGAGCAGCA GCGAGGAGTC AGACAGCGAG
    GAGGAGACAC AAGCTGTCAA GACCCCAGTG CAGGCAAAGC CCTCTGGGAA GATCCCCCAG
    GTTAAAGCTG CCTCTGCAGC ACCCACCCAG GTGCTTTCCC CTAAGAAAGG GGCCCCTCCA
    GCACCCGCTG GCAAGGCAGG GCCTGCAGCC ACCCAAGCCC AGCCCAGGAA GCAGGAGAAG
    GAGGAGAGCA GCAGCAGCAG CAGCGAGGAA TCAGACAGCG AGGGCGAGGC GCCCACGGCC
    ACGCGGCAGA CTACAAGCCT GGCTCAGGCA AAGCCCTTGG AGAAAAACTC TCAGGTCAGA
    GCTGCCTCAG CCACGGGTGC AGGACCCTCG GCGAAGGGCA CAGTCCCTGC GCCCCCTCAG
    AAGGCAAGGT CTGTGGCCGC CCAGGACGGG AAGCAGGAGG ACTCGGAGAG CAGCAGTGAG
    GAGGAGTCGG ACAGCGAGGA GGAGATGCAG AGGGCAGGGA CCTCAGCCCA GGCACAGTCC
    TCGGGGAAAG CCCTCCAGGT CAGACCTTCC TCAGGTCCCA CCAAGGGGCC CCCTCAGAAG
    GCCGGGCCTG CAACCACCCA GGTCAAGGTC GAAAGGGCCG AGGAAGACTC GGAGAGCAGT
    GAGGAGGAAT CAGACAGTGA GGACGAAGCA CCTGTAGCCA AGACTCCAGC CCAGGCAAAA
    TCAACTGTGA AAACTTCTCA GATCAAGGCC TCCCCAAGGA AGGGCACCCC CATTACACCC
    ACATCTGCCA GGGTCCCCCC AGTGCAAGTC GGCACACCAT CCCCCCGGAC AGCAGCAACA
    GTGAGTACGC CCACCTGTGC ATCATCCCCA GCCATAGTCA GGCACACCCA GAAGCCAGGG
    GAGGACTCTT CAAGCAGCGA GGAGTCTGAG AGCGAGGAGG AGACAGCTCC TGCTGCAACA
    GGGGGACAGA TGAAGTCTGT AGGGAAGAGC CTCCCAGTGA AAGCTGCCTC AGCATCCACC
    AAGGGGCCCT CAGGAAAAGG GACCACCCCA GGGCCCCCTG GGAAGGCAGG ACCCCCCGTG
    GCACAGGTCA AGGCTGAGGT GCAGGAAGAC TCAGAGAGCA GTGAGGAGTC GGACAGTGAG
    GAAGCGGCTG CAGCTCCCGC ACAGGTGAAG ACTTCAGTGA AAACCCCTCA GACCAAAGCC
    AACCCTTCTG CCACCAGAGT GGCTTCAGCC AAAGCAGCAG CACCAGCTCC TGGAAAGGGG
    GGCCTTGCTG TTGCTCAAGC CAAAGTGGGG TCCCCAGCCA AGGTAACCCC AGCAGCGAGA
    GCCCCCCAGA GCAGCGCCGT CTCTGGGAGC GGCCAGGTGT CCATGCCGGC TGTGGGGAAG
    GCAGCGGCTG CAGCAGCCCA GGCCCAGCCG GGGCCTGTCG GGGGCTCACA GGAGGACTCG
    GAGAGCAGTG AGGAGGAGTC GGACAGTGAG GGAGAGGCAC CTCCTCAGGC GAAGCCCTCA
    GGAAAGACCC CCCAGGTCAA AACTGCCTCA GCCCCCACCA AGGCGTGCCC CAAGAAAGGG
    GCTGCCCCAG TACCCCCTGG GAAGGCAGGA CCCCCAGCCG CCCAGGCCAG GAGGCAGGAG
    GAGGACTCGG AGAGCAGCAG CGAGGAGGAG TCAGACAGTG ACGGGGACGT GCCAGCAGCT
    GCACCTCCAG CCCAGGTGAG ACCCCAAACC AGGGCCTCCC CAGTAAAAGG TGGCTTTGGT
    ACTCCCATAT CTGCCAAGGC CTCCATGATG CACATGGGCC CCACCCGCCC CCTAACGAAC
    ACCCCCTTGC CCACCATGTT CATGTCCCCT CAGAAGGAGG GTAGCTCCAA AACTGCCACA
    AGCAAGACCC TGACTCCAGC ATCCCTGGAG AAGAAAGCAG AGGAGTCCTC AACCAGCAGT
    GATGAGGACC TGCCGTCGAA CCAGGTGATT AAAACCCCTC TGATTTTTGT CGACCCTAAT
    CGTAGTCCAG CTAGCCCAGC TGCTACCCCC ACCCAAGCGC AGGCTACAGG CACCCCAAGG
    AAGGCTCAGG CCTTGGAGAG CACAACCAGG AGCTCCTCTG AGAGCGAGGA CGAGGACGTG
    ATCCCCGCTA CACAGTGCCC GACTCCTGCC ACTAGGACCA GCGTGGTGAC TGTGCCCACA
    GCACCTCCAA AGGCGGCCCT CAGAGCCAGT GTGGTGGGGG CTCCCAGCAG TGAGGACACA
    AGTCAGGGGT CTGAAGGCAA GAAGCAGGAG GCCTCAGCCA CTCAGGTGAC CAAGAGGAAC
    CTCCCACTGA CGCAGGCTGC CCTGAAGGTT CTTGCCCAGA AAGCCAGTGA GGCCCAGCCT
    CCTGCTGCCA AGACCCCGTC TTCAAGTGGG ACTGACAGTG CTGTGGGAAC TCTCTCCACG
    GTGAGTCCCC AGAGCACCCC TACCCAGGTC AGAATGGCCA ACAAGCTCAG AAAGCCTGAG
    GTTCCTGCAG CTCAGCGGGC CACAGCTACT CCCTCGGGGC ACCCCACACC CAAGGCTCCT
    GCAACCTCAG ATGACAGCAA TAGCAGCTCT TCGGGAAGCG AGGACGAAGC CAAGGGGTCC
    CAGGCGGCCC CGTCAGCCCA CAGGCCAGGC TCAGCCCCCT CCAGGAGGGA GACCTTGGTG
    GAAGAGACCA CAACAGAGTC CAGTGACGAT GAGGTGGTGG CACCTTCCCA GTCTCTCCTC
    TCAGGTTTTG TGACTCCTGG GCTGACTCCA GCTGGTTCCA AGACTTCAAA GGCCACTCCC
    AAACCAGACA CCAGCCCCTC TGTTTCCTCT ACTCCGGCCA CCAGAGATGC CGCAGAAGGC
    AATCAGGAAG TGGAGCCCCA GCAAGCAGCA GGCACCATGT CCCCTAAAAC AGGCAGAAAA
    GAGGCTGGTG CCACACCTCA GAAGCCCGGG AAGGGGCCTG GGAGTACCCC GGCCTCCATG
    CTGGCGCTGC AGAGCAACAT CACCCAGCGC CTCCTGAGCG AGCCCTGGCC CCTGAGTGAG
    GCGCAGGTGC AGGCCTCGGT GGCAAAAGTG CTCACGGAGC TGCTGGAGCA GGAGAGGAAG
    AAGGCCGTGG AGACCGCCAA GGAAGGCAAC CGGAAGGGCC GCCTGGGCCA TAAGCGGAAG
    CTGTCCGAAG ACCAGACAGC CCCCAAGGCC CCCAAGAGCA AGAAAAAGAA GCAGTTGGCA
    GCTGCAGATA GTGGGGAGCA TGTGGTCTCC TCAGAAAAGG CGCCCAGGAC TGTCAAAGGG
    AAATCCAAGA AGGACAGAGC AAGTGGTGAC GCCAAGGAGA AGAAGGAGAA GGAGTCTCCC
    GGCTCTCAGG GGGCCAAGGA GAAGCCAGTA GGGGAGCTGG GGACTCCGAA GAGTGACGGG
    GGAGACCACG GCAACCTTAA GATCAAGAAA GAGAAGAAGA AATCTGACAA GAAAAAAAAA
    GACAAGGAGA AAAAGGAAAA GAAGAAGAAA ACAAAAAAGG CCTCCACCAA AGACCCTGGC
    TCACCATCAC AGAAGAAAAA GAAGAGAAAG AAGAAGACAG CAGAGCAGAC TGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011968802.1
    CDS48..4544
    Translation

    Target ORF information:

    RefSeq Version XM_012113412.2
    Organism Ovis aries musimon(Ovis orientalis musimon)
    Definition Ovis aries musimon Treacher Collins-Franceschetti syndrome 1 (TCOF1), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012113412.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGCTGAGG CCAGGAAGCG GCGGGAGCTC CTTCCCCTGA TCTACCAGCA TCTGCTGCAG 
    GCGGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA AATTTTCCTG
    AGTCAGCCTG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACTTC GGAGATTGGC
    CGGAAACGGA AGGCAGAGGA AGATGCGGCC TTGCAGGCCA AGAGGACCCG CGTGTCAGAC
    CCCATCAGCA GCTCAGAGAG CTCGGAAGAG GAGGAGGAGG AGGAGGAGGC AGAAGCCAAA
    ACCACCAAAC CCACCCCGAG ATTAGCAGCT ACCAACTCCT CAGTCACAGG GACAGTTTCG
    TCTTCAAATG TGAAAGAAAA AGCCAAGGCA AAGACCACGA AAGCCAGCAA GACGGTGAAC
    TCCACGACAC ACCCTGCCCC GGGAAAGGCA GTGGCCCACC TCCTCACTGG GAAGTCACCC
    CAGAAGACGA CGGGGCCCTC GGCAAATGCT GTCTTGGTGT CAGAAACTGA GGAGGAGGGC
    AGCGTGTCAG CTCTCAGAAC CGCTGCCACG CCTGGAATGG CATCTGCCGA CCAGGCTGAT
    AGCTCCAGTG AGGATACCAC CAGCTCGAGC GATGAGACGG ACTTGGAGGT GAAAGCCGCA
    GTAAAACCAC TGCAGGTCAG AGCCTCAGCC ACTGCAGTCA AGGAGTCTCC GAGAAAAAGT
    GCAGCCCCAA CTCCAGGGAA GGCAGGGGTT GTGACACCCC AAGTCAAAGG CGGTGCTGTA
    ACCCCAGCCA GCAGGGCCAA GAAGCCAGAA GAGGTCTCAG AGAGCAGCGA GGAGTCCGAT
    GAGAGTGAGG AGGAGGCCCC GGTGAAGGCC TTGGAGAAAA CCACCCCAAT CAGAGTTGCT
    CCTAGTCCTG CCAAGGGGAC CCCTGGGAAA GGGGCCACCC CAGCAGCCCC TGGGAAGGCA
    GGGCCCTTAG GGAAGCCAGA GGAGGACTCG GAGAGCAGCA GCGAGGAGTC AGACAGCGAG
    GAGGAGACAC AAGCTGTCAA GACCCCAGTG CAGGCAAAGC CCTCTGGGAA GATCCCCCAG
    GTTAAAGCTG CCTCTGCAGC ACCCACCCAG GTGCTTTCCC CTAAGAAAGG GGCCCCTCCA
    GCACCCGCTG GCAAGGCAGG GCCTGCAGCC ACCCAAGCCC AGCCCAGGAA GCAGGAGAAG
    GAGGAGAGCA GCAGCAGCAG CAGCGAGGAA TCAGACAGCG AGGGCGAGGC GCCCACGGCC
    ACGCGGCAGA CTACAAGCCT GGCTCAGGCA AAGCCCTTGG AGAAAAACTC TCAGGTCAGA
    GCTGCCTCAG CCACGGGTGC AGGACCCTCG GCGAAGGGCA CAGTCCCTGC GCCCCCTCAG
    AAGGCAAGGT CTGTGGCCGC CCAGGACGGG AAGCAGGAGG ACTCGGAGAG CAGCAGTGAG
    GAGGAGTCGG ACAGCGAGGA GGAGATGCAG AGGGCAGGGA CCTCAGCCCA GGCACAGTCC
    TCGGGGAAAG CCCTCCAGGT CAGACCTTCC TCAGGTCCCA CCAAGGGGCC CCCTCAGAAG
    GCCGGGCCTG CAACCACCCA GGTCAAGGTC GAAAGGGCCG AGGAAGACTC GGAGAGCAGT
    GAGGAGGAAT CAGACAGTGA GGACGAAGCA CCTGTAGCCA AGACTCCAGC CCAGGCAAAA
    TCAACTGTGA AAACTTCTCA GATCAAGGCC TCCCCAAGGA AGGGCACCCC CATTACACCC
    ACATCTGCCA GGGTCCCCCC AGTGCAAGTC GGCACACCAT CCCCCCGGAC AGCAGCAACA
    GTGAGTACGC CCACCTGTGC ATCATCCCCA GCCATAGTCA GGCACACCCA GAAGCCAGGG
    GAGGACTCTT CAAGCAGCGA GGAGTCTGAG AGCGAGGAGG AGACAGCTCC TGCTGCAACA
    GGGGGACAGA TGAAGTCTGT AGGGAAGAGC CTCCCAGTGA AAGCTGCCTC AGCATCCACC
    AAGGGGCCCT CAGGAAAAGG GACCACCCCA GGGCCCCCTG GGAAGGCAGG ACCCCCCGTG
    GCACAGGTCA AGGCTGAGGT GCAGGAAGAC TCAGAGAGCA GTGAGGAGTC GGACAGTGAG
    GAAGCGGCTG CAGCTCCCGC ACAGGTGAAG ACTTCAGTGA AAACCCCTCA GACCAAAGCC
    AACCCTTCTG CCACCAGAGT GGCTTCAGCC AAAGCAGCAG CACCAGCTCC TGGAAAGGGG
    GGCCTTGCTG TTGCTCAAGC CAAAGTGGGG TCCCCAGCCA AGGTAACCCC AGCAGCGAGA
    GCCCCCCAGA GCAGCGCCGT CTCTGGGAGC GGCCAGGTGT CCATGCCGGC TGTGGGGAAG
    GCAGCGGCTG CAGCAGCCCA GGCCCAGCCG GGGCCTGTCG GGGGCTCACA GGAGGACTCG
    GAGAGCAGTG AGGAGGAGTC GGACAGTGAG GGAGAGGCAC CTCCTCAGGC GAAGCCCTCA
    GGAAAGACCC CCCAGGTCAA AACTGCCTCA GCCCCCACCA AGGCGTGCCC CAAGAAAGGG
    GCTGCCCCAG TACCCCCTGG GAAGGCAGGA CCCCCAGCCG CCCAGGCCAG GAGGCAGGAG
    GAGGACTCGG AGAGCAGCAG CGAGGAGGAG TCAGACAGTG ACGGGGACGT GCCAGCAGCT
    GCACCTCCAG CCCAGGTGAG ACCCCAAACC AGGGCCTCCC CAGTAAAAGG TGGCTTTGGT
    ACTCCCATAT CTGCCAAGGC CTCCATGATG CACATGGGCC CCACCCGCCC CCTAACGAAC
    ACCCCCTTGC CCACCATGTT CATGTCCCCT CAGAAGGAGG GTAGCTCCAA AACTGCCACA
    AGCAAGACCC TGACTCCAGC ATCCCTGGAG AAGAAAGCAG AGGAGTCCTC AACCAGCAGT
    GATGAGGACC TGCCGTCGAA CCAGGTGATT AAAACCCCTC TGATTTTTGT CGACCCTAAT
    CGTAGTCCAG CTAGCCCAGC TGCTACCCCC ACCCAAGCGC AGGCTACAGG CACCCCAAGG
    AAGGCTCAGG CCTTGGAGAG CACAACCAGG AGCTCCTCTG AGAGCGAGGA CGAGGACGTG
    ATCCCCGCTA CACAGTGCCC GACTCCTGCC ACTAGGACCA GCGTGGTGAC TGTGCCCACA
    GCACCTCCAA AGGCGGCCCT CAGAGCCAGT GTGGTGGGGG CTCCCAGCAG TGAGGACACA
    AGTCAGGGGT CTGAAGGCAA GAAGCAGGAG GCCTCAGCCA CTCAGGTGAC CAAGAGGAAC
    CTCCCACTGA CGCAGGCTGC CCTGAAGGTT CTTGCCCAGA AAGCCAGTGA GGCCCAGCCT
    CCTGCTGCCA AGACCCCGTC TTCAAGTGGG ACTGACAGTG CTGTGGGAAC TCTCTCCACG
    GTGAGTCCCC AGAGCACCCC TACCCAGGTC AGAATGGCCA ACAAGCTCAG AAAGCCTGAG
    GTTCCTGCAG CTCAGCGGGC CACAGCTACT CCCTCGGGGC ACCCCACACC CAAGGCTCCT
    GCAACCTCAG ATGACAGCAA TAGCAGCTCT TCGGGAAGCG AGGACGAAGC CAAGGGGTCC
    CAGGCGGCCC CGTCAGCCCA CAGGCCAGGC TCAGCCCCCT CCAGGAGGGA GACCTTGGTG
    GAAGAGACCA CAACAGAGTC CAGTGACGAT GAGGTGGTGG CACCTTCCCA GTCTCTCCTC
    TCAGGTTTTG TGACTCCTGG GCTGACTCCA GCTGGTTCCA AGACTTCAAA GGCCACTCCC
    AAACCAGACA CCAGCCCCTC TGTTTCCTCT ACTCCGGCCA CCAGAGATGC CGCAGAAGGC
    AATCAGGAAG TGGAGCCCCA GCAAGCAGCA GGCACCATGT CCCCTAAAAC AGGCAGAAAA
    GAGGCTGGTG CCACACCTCA GAAGCCCGGG AAGGGGCCTG GGAGTACCCC GGCCTCCATG
    CTGGCGCTGC AGAGCAACAT CACCCAGCGC CTCCTGAGCG AGCCCTGGCC CCTGAGTGAG
    GCGCAGGTGC AGGCCTCGGT GGCAAAAGTG CTCACGGAGC TGCTGGAGCA GGAGAGGAAG
    AAGGCCGTGG AGACCGCCAA GGAAGGCAAC CGGAAGGGCC GCCTGGGCCA TAAGCGGAAG
    CTGTCCGAAG ACCAGACAGC CCCCAAGGCC CCCAAGAGCA AGAAAAAGAA GCAGTTGGCA
    GCTGCAGATA GTGGGGAGCA TGTGGTCTCC TCAGAAAAGG CGCCCAGGAC TGTCAAAGGG
    AAATCCAAGA AGGACAGAGC AAGTGGTGAC GCCAAGGAGA AGAAGGAGAA GGAGTCTCCC
    GGCTCTCAGG GGGCCAAGGA GAAGCCAGTA GGGGAGCTGG GGACTCCGAA GAGTGACGGG
    GGAGACCACG GCAACCTTAA GATCAAGAAA GAGAAGAAGA AATCTGACAA GAAAAAAAAA
    GACAAGGAGA AAAAGGAAAA GAAGAAGAAA ACAAAAAAGG CCTCCACCAA AGACCCTGGC
    TCACCATCAC AGAAGAAAAA GAAGAGAAAG AAGAAGACAG CAGAGCAGAC TGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OSm49869
    Clone ID Related Accession (Same CDS sequence) XM_015096143.1
    Accession Version XM_015096143.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4098bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-16
    Organism Ovis aries(sheep)
    Product treacle protein isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014639014.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGCATCTG CCGACCAGGC TGATAGCTCC AGTGAGGATA CCACCAGCTC GAGCGATGAG 
    ACGGACTTGG AGGTGAAAGC CGCAGTAAAA CCACTGCAGG TCAGAGCCTC AGCCACTGCA
    GTCAAGGAGT CTCCGAGAAA AAGTGCAGCC CCAACTCCAG GGAAGGCAGG GGTTGTGACA
    CCCCAAGTCA AAGGCGGTGC TGTAACCCCA GCCAGCAGGG CCAAGAAGCC AGAAGAGGTC
    TCAGAGAGCA GCGAGGAGTC CGATGAGAGT GAGGAGGAGG CCCCGGTGAA GGCCTTGGAG
    AAAACCACCC CAATCAGAGT TGCTCCTAGT CCTGCCAAGG GGACCCCTGG GAAAGGGGCC
    ACCCCAGCAG CCCCTGGGAA GGCAGGGCCC TTAGGGAAGC CAGAGGAGGA CTCGGAGAGC
    AGCAGCGAGG AGTCAGACAG CGAGGAGGAG ACACAAGCTG TCAAGACCCC AGTGCAGGTG
    GGACCTGGAG AGAGGGCCCC GGAGCTGGCA CCCACCCCAG GAGGCCTCCT GAGGGCTGAT
    TCTCCCACTC CACGTGCCCT GGCCACGCAA GGGCCACGTG GAGTCTGTGG TGTCTTTGAC
    CCCGTCTGCT ATCCTGTTTC CTCAAGTCCA CTGTCTGGGC TCTCTCCACT GATTCTGTTT
    CTCTCTCTTC AGGCAAAGCC CTCTGGGAAG ATCCCCCAGG TTAAAGCTGC CTCTGCAGCA
    CCCACCCAGG TGCTTTCCCC TAAGAAAGGG GCCCCTCCAG CACCCGCTGG CAAGGCAGGG
    CCTGCAGCCA CCCAAGCCCA GCCCAGGAAG CAGGAGAAGG AGGAGAGCAG CAGCAGCAGC
    AGCGAGGAAT CAGACAGCGA GGGCGAGGCG CCCACGGCCA CGCGGCAGAC TACAAGCCTG
    GCTCAGGCAA AGCCCTTGGA GAAAAACTCT CAGGTCAGAG CTGCCTCAGC CACGGGTGCA
    GGACCCTCGG CGAAGGGCGC AGTCCCTGCG CCCCCTCAGA AGGCAAGGTC TGTGGCCGCC
    CAGGACGGGA AGCAGGAGGA CTCGGAGAGC AGCAGTGAGG AGGAGTCGGA CAGCGAGGAG
    GAGATGCAGA GGGCAGGGAC CTCAGCCCAG GCACAGTCCT CGGGGAAAGC CCTCCAGGTC
    AGACCTTCCT CAGGTCCCAC CAAGGGGCCC CCTCAGAAGG CCGGGCCTGC AACCACCCAG
    GTCAAGGTCG AAAGGGCCGA GGAAGACTCG GAGAGCAGTG AGGAGGAATC AGACAGTGAG
    GACGAAGCAC CTGTAGCCAA GACTCCAGCC CAGGCAAAAT CAACTGTGAA AACTTCTCAG
    ATCAAGGCCT CCCCAAGGAA GGGCACCCCC ATTACACCCA CATCTGCCAG GGTCCCCCCA
    GTGCAAGTCG GCACACCATC CCCCCGGACA GCAGCAACAG TGAGTACGCC CACCTGTGCA
    TCATCCCCAG CCATAGTCAG GCACACCCAG AAGCCAGGGG AGGACTCTTC AAGCAGCGAG
    GAGTCTGAGA GCGAGGAGGA GACAGCTCCT GCTGCAACAG GGGGACAGAT GAAGTCTGTA
    GGGAAGAGCC TCCCAGTGAA AGCTGCCTCA GCATCCACCA AGGGGCCCTC AGGAAAAGGG
    ACCACCCCAG GGCCCCCTGG GAAGGCAGGA CCCCCCGTGG CACAGGTCAA GGCTGAGGTG
    CAGGAAGACT CAGAGAGCAG TGAGGAGTCG GACAGTGAGG AAGCGGCTGC AGCTCCCGCA
    CAGGTGAAGA CTTCAGTGAA AACCCCTCAG ACCAAAGCCA ACCCTTCTGC CACCAGAGTG
    GCTTCAGCCA AAGCAGCAGC ACCAGCTCCT GGAAAGGGGG GCCTTGCTGT TGCTCAAGCC
    AAAGTGGGGT CCCCAGCCAA GGTAACCCCA GCAGCGAGAG CCCCCCAGAG CAGCGCCGTC
    TCTGGGAGCA GCCAGGTGTC CATGCCGGCT GTGGGGAAGG CAGCGGCTGC AGCAGCCCAG
    GCCCAGCCGG GGCCTGTCGG GGGCTCACAG GAGGACTCGG AGAGCAGTGA GGAGGAGTCG
    GACAGTGAGG GAGAGGCACC TCCTCAGGCG AAGCCCTCAG GAAAGACCCC CCAGGTCAAA
    ACTGCCTCAG CCCCCACCAA GGCGTGCCCC AAGAAAGGGG CTGCCCCAGT ACCCCCTGGG
    AAGGCAGGAC CCCCAGCCGC CCAGGCCAGG AGGCAGGAGG AGGACTCGGA GAGCAGCAGC
    GAGGAGGAGT CAGACAGTGA CGGGGACGTG CCAGCAGCTG CACCTCCAGC CCAGAAGGAG
    GGTAGCTCCA AAACTGCCAC AAGCAAGACC CTGACTCCAG CATCCCTGGA GAAGAAAGCA
    GAGGAGTCCT CAACCAGCAG TGATGAGGAC CTGCCGTCGA ACCAGGTGAT TAAAACCCCT
    CTGATTTTTG TCGACCCTAA TCGTAGTCCA GCTAGCCCAG CTGCTACCCC CACCCAAGCG
    CAGGCTACAG GCACCCCAAG GAAGGCTCAG GCCTTGGAGA GCACAGCCAG GAGCTCCTCT
    GAGAGCGAGG ACGAGGACGT GATCCCCGCT ACACAGTGCC CGACTCCTGC CACTAGGACC
    AGCGTGGTGA CTGTGCCCAC AGCACCTCCA AAGGCGGCCC TCAGAGCCAG TGTGGTGGGG
    GCTCCCAGCA GTGAGGACAC AAGTCAGGGG TCTGAAGGCA AGAAGCAGGA GGCCTCAGCC
    ACTCAGGTGA CCAAGAGGAA CCTCCCACTG ACGCAGGCTG CCCTGAAGGT TCTTGCCCAG
    AAAGCCAATG AGGCCCAGCC TCCTGCTGCC AAGACCCCGT CTTCAAGTGG GACTGACAGT
    GCTGTGGGAA CTCTCTCCAC GGTGAGTCCC CAGAGCACCC CTACCCAGGT CAGAATGGCC
    AACAAGCTCA GAAAGCCTGA GGTTCCTGCA GCTCAGCGGG CCACAGCTAC TCCCTCGGGG
    CACCCCACAC CCAAGGCTCC TGCAGCCTCA GATGACAGCA ATAGCAGCTC TTCGGGAAGC
    GAGGACGAAG CCAAGGGGTC CCAGGCGGCC CCGTCAGCCC ACAGGCCAGG CTCAGCCCCC
    TCCAGGAGGG AGACCTTGGT GGAAGAGACC ACAACAGAGT CCAGTGACGA TGAGGTGGTG
    GCACCTTCCC AGTCTCTCCT CTCAGGTTTT GTGACTCCTG GGCTGACTCC AGCTGGTTCC
    AAGACTTCAA AGGCCACTCC CAAACCAGAC ACCAGCCCCT CTGTTTCCTC TACTCCGGCC
    ACCAGAGATG CCGCAGAAGG CAATCAGGAA GTGGAGCCCC AGCAAGCAGC AGGCACCATG
    TCCCCTAAAA CAGGCAGAAA AGAGGCTGGT GCCACACCTC AGAAGCCCGG GAAGGGGCCT
    GGGAGTACCC CGGCCTCCAT GCTGGCGCTG CAGAGCAACA TCACCCAGCG CCTCCTGAGC
    GAGCCCTGGC CCCTGAGTGA GGCGCAGGTG CAGGCCTCGG TGGCAAAAGT GCTCACGGAG
    CTGCTGGAGC AGGAGAGGAA GAAGGCCGTG GAGACCGCCA AGGAAGGCAA CCGGAAGGGC
    CGCCTGGGCC ATAAGCGGAA GCTGTCCGAA GACCAGACAG CCCCCAAGGC CCCCAAGAGC
    AAGAAAAAGA AGCAGTTGGC AGCTGCAGAT AGTGGGGAGC ATGTGGTCTC CTCAGAAAAG
    GCGCCCAGGA CTGTCAAAGG GAAATCCAAG AAGGACAGAG CAAGTGGTGA CGCCAAGGAG
    AAGAAGGAGA AGGAGTCTCC CGGCTCTCAG GGGGCCAAGG AGAAGCCAGT AGGGGAGCTG
    GGGACTCCGA AGAGTGACGG GGGAGACCAC GGCAACCTTA AGATCAAGAA AGAGAAGAAG
    AAATCTGACA AGAGATGGGC AAAGCCAGAA CCAGGTCCTG GAGCAGCGGA GCCACCACCA
    CCAGGACGGC GCTCACAGCT CCAGGGCATG TTCCGCTCGA AGAGCCCTGC CCTCCCCCAC
    GGCGGCCACA GCCACGTGCA CTGGCAGCCG AGGCTCCTGG GGTCCAGGCT CCTGGATAGC
    AGCTGCCCCT TGCTGCATTC TGTGCTGGAG TCCTCGTGCA GCCGGGCCAC GTTTAACCCC
    CACCCCAGCC CTGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_014951629.1
    CDS105..4202
    Translation

    Target ORF information:

    RefSeq Version XM_015096143.1
    Organism Ovis aries(sheep)
    Definition Ovis aries Treacher Collins-Franceschetti syndrome 1 (TCOF1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015096143.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGCATCTG CCGACCAGGC TGATAGCTCC AGTGAGGATA CCACCAGCTC GAGCGATGAG 
    ACGGACTTGG AGGTGAAAGC CGCAGTAAAA CCACTGCAGG TCAGAGCCTC AGCCACTGCA
    GTCAAGGAGT CTCCGAGAAA AAGTGCAGCC CCAACTCCAG GGAAGGCAGG GGTTGTGACA
    CCCCAAGTCA AAGGCGGTGC TGTAACCCCA GCCAGCAGGG CCAAGAAGCC AGAAGAGGTC
    TCAGAGAGCA GCGAGGAGTC CGATGAGAGT GAGGAGGAGG CCCCGGTGAA GGCCTTGGAG
    AAAACCACCC CAATCAGAGT TGCTCCTAGT CCTGCCAAGG GGACCCCTGG GAAAGGGGCC
    ACCCCAGCAG CCCCTGGGAA GGCAGGGCCC TTAGGGAAGC CAGAGGAGGA CTCGGAGAGC
    AGCAGCGAGG AGTCAGACAG CGAGGAGGAG ACACAAGCTG TCAAGACCCC AGTGCAGGTG
    GGACCTGGAG AGAGGGCCCC GGAGCTGGCA CCCACCCCAG GAGGCCTCCT GAGGGCTGAT
    TCTCCCACTC CACGTGCCCT GGCCACGCAA GGGCCACGTG GAGTCTGTGG TGTCTTTGAC
    CCCGTCTGCT ATCCTGTTTC CTCAAGTCCA CTGTCTGGGC TCTCTCCACT GATTCTGTTT
    CTCTCTCTTC AGGCAAAGCC CTCTGGGAAG ATCCCCCAGG TTAAAGCTGC CTCTGCAGCA
    CCCACCCAGG TGCTTTCCCC TAAGAAAGGG GCCCCTCCAG CACCCGCTGG CAAGGCAGGG
    CCTGCAGCCA CCCAAGCCCA GCCCAGGAAG CAGGAGAAGG AGGAGAGCAG CAGCAGCAGC
    AGCGAGGAAT CAGACAGCGA GGGCGAGGCG CCCACGGCCA CGCGGCAGAC TACAAGCCTG
    GCTCAGGCAA AGCCCTTGGA GAAAAACTCT CAGGTCAGAG CTGCCTCAGC CACGGGTGCA
    GGACCCTCGG CGAAGGGCGC AGTCCCTGCG CCCCCTCAGA AGGCAAGGTC TGTGGCCGCC
    CAGGACGGGA AGCAGGAGGA CTCGGAGAGC AGCAGTGAGG AGGAGTCGGA CAGCGAGGAG
    GAGATGCAGA GGGCAGGGAC CTCAGCCCAG GCACAGTCCT CGGGGAAAGC CCTCCAGGTC
    AGACCTTCCT CAGGTCCCAC CAAGGGGCCC CCTCAGAAGG CCGGGCCTGC AACCACCCAG
    GTCAAGGTCG AAAGGGCCGA GGAAGACTCG GAGAGCAGTG AGGAGGAATC AGACAGTGAG
    GACGAAGCAC CTGTAGCCAA GACTCCAGCC CAGGCAAAAT CAACTGTGAA AACTTCTCAG
    ATCAAGGCCT CCCCAAGGAA GGGCACCCCC ATTACACCCA CATCTGCCAG GGTCCCCCCA
    GTGCAAGTCG GCACACCATC CCCCCGGACA GCAGCAACAG TGAGTACGCC CACCTGTGCA
    TCATCCCCAG CCATAGTCAG GCACACCCAG AAGCCAGGGG AGGACTCTTC AAGCAGCGAG
    GAGTCTGAGA GCGAGGAGGA GACAGCTCCT GCTGCAACAG GGGGACAGAT GAAGTCTGTA
    GGGAAGAGCC TCCCAGTGAA AGCTGCCTCA GCATCCACCA AGGGGCCCTC AGGAAAAGGG
    ACCACCCCAG GGCCCCCTGG GAAGGCAGGA CCCCCCGTGG CACAGGTCAA GGCTGAGGTG
    CAGGAAGACT CAGAGAGCAG TGAGGAGTCG GACAGTGAGG AAGCGGCTGC AGCTCCCGCA
    CAGGTGAAGA CTTCAGTGAA AACCCCTCAG ACCAAAGCCA ACCCTTCTGC CACCAGAGTG
    GCTTCAGCCA AAGCAGCAGC ACCAGCTCCT GGAAAGGGGG GCCTTGCTGT TGCTCAAGCC
    AAAGTGGGGT CCCCAGCCAA GGTAACCCCA GCAGCGAGAG CCCCCCAGAG CAGCGCCGTC
    TCTGGGAGCA GCCAGGTGTC CATGCCGGCT GTGGGGAAGG CAGCGGCTGC AGCAGCCCAG
    GCCCAGCCGG GGCCTGTCGG GGGCTCACAG GAGGACTCGG AGAGCAGTGA GGAGGAGTCG
    GACAGTGAGG GAGAGGCACC TCCTCAGGCG AAGCCCTCAG GAAAGACCCC CCAGGTCAAA
    ACTGCCTCAG CCCCCACCAA GGCGTGCCCC AAGAAAGGGG CTGCCCCAGT ACCCCCTGGG
    AAGGCAGGAC CCCCAGCCGC CCAGGCCAGG AGGCAGGAGG AGGACTCGGA GAGCAGCAGC
    GAGGAGGAGT CAGACAGTGA CGGGGACGTG CCAGCAGCTG CACCTCCAGC CCAGAAGGAG
    GGTAGCTCCA AAACTGCCAC AAGCAAGACC CTGACTCCAG CATCCCTGGA GAAGAAAGCA
    GAGGAGTCCT CAACCAGCAG TGATGAGGAC CTGCCGTCGA ACCAGGTGAT TAAAACCCCT
    CTGATTTTTG TCGACCCTAA TCGTAGTCCA GCTAGCCCAG CTGCTACCCC CACCCAAGCG
    CAGGCTACAG GCACCCCAAG GAAGGCTCAG GCCTTGGAGA GCACAGCCAG GAGCTCCTCT
    GAGAGCGAGG ACGAGGACGT GATCCCCGCT ACACAGTGCC CGACTCCTGC CACTAGGACC
    AGCGTGGTGA CTGTGCCCAC AGCACCTCCA AAGGCGGCCC TCAGAGCCAG TGTGGTGGGG
    GCTCCCAGCA GTGAGGACAC AAGTCAGGGG TCTGAAGGCA AGAAGCAGGA GGCCTCAGCC
    ACTCAGGTGA CCAAGAGGAA CCTCCCACTG ACGCAGGCTG CCCTGAAGGT TCTTGCCCAG
    AAAGCCAATG AGGCCCAGCC TCCTGCTGCC AAGACCCCGT CTTCAAGTGG GACTGACAGT
    GCTGTGGGAA CTCTCTCCAC GGTGAGTCCC CAGAGCACCC CTACCCAGGT CAGAATGGCC
    AACAAGCTCA GAAAGCCTGA GGTTCCTGCA GCTCAGCGGG CCACAGCTAC TCCCTCGGGG
    CACCCCACAC CCAAGGCTCC TGCAGCCTCA GATGACAGCA ATAGCAGCTC TTCGGGAAGC
    GAGGACGAAG CCAAGGGGTC CCAGGCGGCC CCGTCAGCCC ACAGGCCAGG CTCAGCCCCC
    TCCAGGAGGG AGACCTTGGT GGAAGAGACC ACAACAGAGT CCAGTGACGA TGAGGTGGTG
    GCACCTTCCC AGTCTCTCCT CTCAGGTTTT GTGACTCCTG GGCTGACTCC AGCTGGTTCC
    AAGACTTCAA AGGCCACTCC CAAACCAGAC ACCAGCCCCT CTGTTTCCTC TACTCCGGCC
    ACCAGAGATG CCGCAGAAGG CAATCAGGAA GTGGAGCCCC AGCAAGCAGC AGGCACCATG
    TCCCCTAAAA CAGGCAGAAA AGAGGCTGGT GCCACACCTC AGAAGCCCGG GAAGGGGCCT
    GGGAGTACCC CGGCCTCCAT GCTGGCGCTG CAGAGCAACA TCACCCAGCG CCTCCTGAGC
    GAGCCCTGGC CCCTGAGTGA GGCGCAGGTG CAGGCCTCGG TGGCAAAAGT GCTCACGGAG
    CTGCTGGAGC AGGAGAGGAA GAAGGCCGTG GAGACCGCCA AGGAAGGCAA CCGGAAGGGC
    CGCCTGGGCC ATAAGCGGAA GCTGTCCGAA GACCAGACAG CCCCCAAGGC CCCCAAGAGC
    AAGAAAAAGA AGCAGTTGGC AGCTGCAGAT AGTGGGGAGC ATGTGGTCTC CTCAGAAAAG
    GCGCCCAGGA CTGTCAAAGG GAAATCCAAG AAGGACAGAG CAAGTGGTGA CGCCAAGGAG
    AAGAAGGAGA AGGAGTCTCC CGGCTCTCAG GGGGCCAAGG AGAAGCCAGT AGGGGAGCTG
    GGGACTCCGA AGAGTGACGG GGGAGACCAC GGCAACCTTA AGATCAAGAA AGAGAAGAAG
    AAATCTGACA AGAGATGGGC AAAGCCAGAA CCAGGTCCTG GAGCAGCGGA GCCACCACCA
    CCAGGACGGC GCTCACAGCT CCAGGGCATG TTCCGCTCGA AGAGCCCTGC CCTCCCCCAC
    GGCGGCCACA GCCACGTGCA CTGGCAGCCG AGGCTCCTGG GGTCCAGGCT CCTGGATAGC
    AGCTGCCCCT TGCTGCATTC TGTGCTGGAG TCCTCGTGCA GCCGGGCCAC GTTTAACCCC
    CACCCCAGCC CTGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.