SPTAN1 gene cDNA ORF clone, Ovis aries(sheep)

mRNA	Protein	Name
XM_012147479.2	XP_012002869.1	spectrin alpha chain, non-erythrocytic 1 isoform X4
XM_027966355.1	XP_027822156.1	spectrin alpha chain, non-erythrocytic 1 isoform X2
XM_012147480.2	XP_012002870.1	spectrin alpha chain, non-erythrocytic 1 isoform X4
XM_012147485.1	XP_012002875.1	spectrin alpha chain, non-erythrocytic 1 isoform X8
XM_027966356.1	XP_027822157.1	spectrin alpha chain, non-erythrocytic 1 isoform X3
XM_027966359.1	XP_027822160.1	spectrin alpha chain, non-erythrocytic 1 isoform X6
XM_027966360.1	XP_027822161.1	spectrin alpha chain, non-erythrocytic 1 isoform X7
XM_027966363.1	XP_027822164.1	spectrin alpha chain, non-erythrocytic 1 isoform X10
XM_012147478.1	XP_012002868.1	spectrin alpha chain, non-erythrocytic 1 isoform X3
XM_012147483.1	XP_012002873.1	spectrin alpha chain, non-erythrocytic 1 isoform X6
XM_012147486.1	XP_012002876.1	spectrin alpha chain, non-erythrocytic 1 isoform X9
XM_012147487.1	XP_012002877.1	spectrin alpha chain, non-erythrocytic 1 isoform X10
XM_015093852.1	XP_014949338.1	spectrin alpha chain, non-erythrocytic 1 isoform X1
XM_012147477.1	XP_012002867.1	spectrin alpha chain, non-erythrocytic 1 isoform X2
XM_012147482.1	XP_012002872.1	spectrin alpha chain, non-erythrocytic 1 isoform X5
XM_012147484.1	XP_012002874.1	spectrin alpha chain, non-erythrocytic 1 isoform X7
XM_012147488.1	XP_012002878.1	spectrin alpha chain, non-erythrocytic 1 isoform X11
XM_027966354.1	XP_027822155.1	spectrin alpha chain, non-erythrocytic 1 isoform X1
XM_027966357.1	XP_027822158.1	spectrin alpha chain, non-erythrocytic 1 isoform X4
XM_027966358.1	XP_027822159.1	spectrin alpha chain, non-erythrocytic 1 isoform X5
XM_027966361.1	XP_027822162.1	spectrin alpha chain, non-erythrocytic 1 isoform X8
XM_027966362.1	XP_027822163.1	spectrin alpha chain, non-erythrocytic 1 isoform X9

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OOv24449	XM_012147477.1 Latest version!	Ovis aries musimon spectrin, alpha, non-erythrocytic 1 (SPTAN1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
OOv24450	XM_012147478.1 Latest version!	Ovis aries musimon spectrin, alpha, non-erythrocytic 1 (SPTAN1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
OOv24451	XM_012147480.2 Latest version!	Ovis aries musimon spectrin, alpha, non-erythrocytic 1 (SPTAN1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
OOv24451	XM_012147479.2 Latest version!	Ovis aries musimon spectrin, alpha, non-erythrocytic 1 (SPTAN1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
OOv24452	XM_012147482.1 Latest version!	Ovis aries musimon spectrin, alpha, non-erythrocytic 1 (SPTAN1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
OOv24453	XM_012147483.1 Latest version!	Ovis aries musimon spectrin, alpha, non-erythrocytic 1 (SPTAN1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
OOv24454	XM_012147484.1 Latest version!	Ovis aries musimon spectrin, alpha, non-erythrocytic 1 (SPTAN1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
OOv24455	XM_012147485.1 Latest version!	Ovis aries musimon spectrin, alpha, non-erythrocytic 1 (SPTAN1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
OOv24456	XM_012147486.1 Latest version!	Ovis aries musimon spectrin, alpha, non-erythrocytic 1 (SPTAN1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
OOv24457	XM_012147487.1 Latest version!	Ovis aries musimon spectrin, alpha, non-erythrocytic 1 (SPTAN1), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
OOv24458	XM_012147488.1 Latest version!	Ovis aries musimon spectrin, alpha, non-erythrocytic 1 (SPTAN1), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
OSm48298	XM_015093852.1 Latest version!	Ovis aries spectrin, alpha, non-erythrocytic 1 (SPTAN1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
OSm76648	XM_027966354.1 Latest version!	Ovis aries spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OSm76649	XM_027966355.1 Latest version!	Ovis aries spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OSm76650	XM_027966356.1 Latest version!	Ovis aries spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OSm76651	XM_027966357.1 Latest version!	Ovis aries spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OSm76652	XM_027966358.1 Latest version!	Ovis aries spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OSm76653	XM_027966359.1 Latest version!	Ovis aries spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OSm76654	XM_027966360.1 Latest version!	Ovis aries spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OSm76655	XM_027966361.1 Latest version!	Ovis aries spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OSm76656	XM_027966362.1 Latest version!	Ovis aries spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OSm76657	XM_027966363.1 Latest version!	Ovis aries spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00

SPTAN1 ( XM_012147477.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_012002867.1 Ovis aries musimon spectrin, alpha, non-erythrocytic 1 (SPTAN1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OOv24449
Clone ID Related Accession (Same CDS sequence)	XM_012147477.1
Accession Version	XM_012147477.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7533bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	spectrin alpha chain, non-erythrocytic 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942583.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTCAT CGTTTCGTAA ACGCAGAGTC CAGAAAATGG ACCCAAGCGG GGTCAAAGTG 
CTGGAGACAG CCGAGGACAT CCAGGAGAGG CGGCAGCAGG TCCTGGACAG ATACCACCGC 
TTCAAGGAGC TCTCGACCCT GAGGCGCCAG AAGCTTGAAG ACTCCTACCG ATTCCAGTTT 
TTCCAAAGAG ATGCTGAGGA GCTGGAGAAA TGGATTCAGG AGAAGCTTCA GATTGCCTCG 
GATGAGAATT ACAAAGACCC AACCAACCTG CAGGGCAAGC TGCAGAAGCA TCAAGCCTTC 
GAAGCCGAGG TGCAGGCCAA CTCGGGTGCC ATCGTGAAGC TTGATGAGAC CGGTAACCTG 
ATGATCTCAG AAGGGCACTT CGCGTCCGAA ACCATCCGGA CTCGTCTGAT GGAGCTGCAC 
CGCCAGTGGG AATTGCTTCT GGAGAAGATG CGGGAGAAAG GCATCAAGCT GCTGCAGGCC 
CAGAAGCTGG TGCAGTACCT GCGGGAGTGT GAGGACGTGA TGGACTGGAT CAATGACAAG 
GAAGCCATTG TGACATCTGA GGAGCTGGGC CAGGACCTGG AGCACGTGGA GGTGTTACAG 
AAGAAATTCG AAGAGTTTCA GACAGACATG GCTGCCCACG AAGAGAGAGT CAATGAAGTG 
AACCAGTTTG CTGCCAAGCT CATTCAGGAG CAGCACCCTG AGGAGGAGCT GATCAAGACA 
AAGGAGGACG AGGTGAATGC AGCCTGGCAG CGGCTGAAGG GCCTGGCCCT CCAGCGGCAG 
GGGAAGCTGT TCGGGGCAGC TGAGGTCCAA CGCTTTAACA GGGATGTGGA CGAGACCATT 
AGCTGGATTA AGGAGAAGGA ACAGTTAATG GCCTCTGATG ACTTTGGCCG GGACCTGGCC 
AGTGTCCAGG CTCTACTGCG GAAACACGAG GGTTTGGAGA GAGATCTTGC TGCCTTGGAG 
GACAAGGTCA AAGCCCTGTG TGCCGAGGCT GACCGCCTGC AGCAGTCCCA CCCTCTGAGT 
GCCACCCAGA TCCAAGTGAA GCGAGAGGAG CTGATCACCA ACTGGGAGCA GATCCGCACC 
CTGGCCGCGG AGAGACACGC ACGTCTCAAC GATTCCTACA GGCTTCAGCG CTTCCTTGCT 
GACTTCCGAG ACCTCACCAG CTGGGTCACT GAGATGAAAG CCCTCATCAA TGCAGATGAA 
CTTGCCAATG ATGTGGCCGG GGCCGAAGCC CTGCTGGATA GGCATCAGGA GCACAAGGGT 
GAAATTGACG CTCATGAAGA TAGCTTTAAA TCTGCAGATG AGTCTGGACA GGCCCTGCTT 
GCAGCTGGTC ACTATGCCTC AGATGAAGTG AGGGAGAAGC TGACCATCCT GTCCGAGGAG 
CGGGCCGCGC TACTAGAGCT CTGGGAGCTG CGACGGCAGC AATATGAGCA GTGCATGGAC 
CTGCAGCTCT TCTACCGCGA CACGGAGCAG GTGGACAACT GGATGAGCAA GCAGGAGGCG 
TTCCTGTTGA ACGAAGACCT GGGTGATTCC TTGGATAGTG TGGAAGCGCT TCTCAAGAAG 
CACGAAGACT TTGAGAAGTC CCTCAGCGCC CAGGAGGAGA AGATCACAGC CTTAGATGAG 
TTTGCCACCA AGCTGATTCA GAACAACCAC TACGCGATGG AAGATGTGGC CACCCGGCGA 
GACGCACTGC TGAGCCGCCG CAACGCCCTC CATGAGAGAG CCATGTACCG CCGTGCCCAG 
CTTGCAGATT CCTTCCACTT GCAGCAGTTT TTCCGTGACT CTGATGAGCT CAAGAGTTGG 
GTCAACGAGA AGATGAAAAC AGCCACAGAT GAAGCTTATA AAGACCCATC CAACCTACAA 
GGGAAAGTGC AGAAGCACCA GGCCTTTGAG GCCGAGCTCT CAGCAAACCA GAGCCGCATC 
GACGCCTTGG AGAAAGCTGG GCAGAAGCTG ATCGACGTTG ACCACTATGC CAAGGATGAG 
GTGGCGACTC GGATGAATGA AGTTATCAGT CTGTGGAAGA AGCTGCTGGA GGCCACGGAG 
CTGAAAGGCA TAAAGCTCCG TGAGGCTAAC CAGCAGCAGC AGTTTAACCG CAATGTTGAG 
GACATCGAGC TGTGGCTGTA CGAAGTGGAA GGTCATCTGG CCTCGGATGA CTACGGCAAA 
GACCTCACCA ACGTCCAGAA CCTCCAGAAA AAGCATGCCC TGCTGGAGGC TGACGTGGCC 
GCTCACCAGG ATCGCATCGA TGGCATCACT ATCCAAGCCC GCCAGTTCCA GGATGCGGGC 
CACTTTGATG CTGAAAACAT CAAGAAGAAG CAGGAGGCCC TGGTAGCTCG CTACGAGGCA 
CTCAAGGAGC CCATGGTTGC CCGGAAGCAG AAGCTGGCCG ATTCCCTGCG GTTGCAGCAG 
CTCTTCCGTG ACGTTGAGGA TGAGGAGACG TGGATTCGGG AGAAAGAACC TATTGCCGCC 
TCCACCAACA GAGGCAAAGA TTTAATTGGA GTCCAGAACC TGCTAAAGAA ACACCAAGCG 
CTACAAGCAG AAATTGCTGG CCACGAGCCC CGCATCAAAG CAGTCACACA GAAGGGGAAC 
GCCATGGTAG AGGAGGGCCA TTTTGCTGCC GAGGACGTGA AGGCCAAGCT CCATGAACTG 
AACCAGAAGT GGGAGTCGCT GAAGTCCAAG GCCTCCCAGC GACGGCAGGA CCTGGAGGAC 
TCGCTGCAGG CCCAGCAGTA CTTCGCTGAT GCCAACGAGG CCGAGTCCTG GATGCGGGAG 
AAGGAGCCCA TCGTGGGCAG CACCGACTAT GGCAAAGATG AGGACTCTGC CGAGGCTCTG 
CTAAAGAAGC ACGAGGCATT GATGTCGGAT CTCAGCGCCT ATGGCAGCAG CATCCAGGCC 
CTGCGAGAGC AGGCCCAGTC GTGCCGGCAA CAAGTGGCCC CCATGGATGA CGAGACTGGG 
AAGGAGCTGG TCTTGGCGCT CTATGACTAT CAGGAGAAGA GTCCCCGAGA GGTCACCATG 
AAGAAAGGAG ACATTCTCAC CTTACTCAAC AGCACCAACA AGGACTGGTG GAAAGTGGAA 
GTGAACGACC GGCAGGGCTT CGTGCCGGCC GCCTACGTGA AGAAGCTGGA CCCAGCGCAG 
TCGGCCTCCC GGGAGAACCT CCTGGAAGAG CAGGGCAGCA TCGCGCTGCG GCAGGAGCAG 
ATTGACAACC AGACGCGCGT AACTAAGGAG GCCGGCAGCG TGTCTCTGCG TATGAAACAG 
GTGGAAGAAC TGTACCATTC TCTGCTGGAA CTGGGAGAGA AGCGTAAAGG CATGTTGGAG 
AAGAGCTGTA AGAAGTTCAT GCTGTTCCGT GAAGCGAACG AGCTGCAGCA GTGGATCAAC 
GAGAAGGAAG CGGCCCTGAC AAGCGAGGAG GTCGGCGCGG ACTTGGAGCA GGTCGAAGTG 
CTGCAGAAGA AGTTTGACGA CTTCCAGAAG GATCTCAAGG CCAATGAGTC ACGGCTGAAG 
GACATTAACA AGGTGGCTGA AGACCTGGAG TCGGAAGGCC TGATGGCGGA GGAGGTGCAG 
GCCGTGCAGC AGCAGGAGGT GTATGGCGCG ATGCCCAGGG ATGAATCTGA TTCCAAGACA 
GCCTCCCCGT GGAAGTCTGC TCGCCTGATG GTTCACACCG TGGCCACCTT TAATTCCATC 
AAGGAGCTCA ATGAGCGCTG GCGGTCCCTG CAGCAGCTGG CTGAGGAGCG GAGCCAACTC 
TTGGGCAGCG CCCACGAAGT GCAGCGGTTC CACAGAGATG CTGACGAAAC CAAAGAGTGG 
ATCGAGGAGA AGAACCAGGC TCTAAACACA GACAACTATG GCCACGACCT GGCCAGCGTC 
CAGGCCCTGC AGCGCAAGCA TGAGGGCTTC GAGAGGGACC TCGCGGCCCT TGGCGACAAG 
GTGAACTCGC TCGGGGAGAC AGCCGAGCGC CTGATCCAGT CCCACCCCGA GTCGGCAGAG 
GACCTGCAGG AGAAGTGCAC TGAGCTGAAC CAGGCATGGA GCAGCCTGGG CAAGCGTGCC 
GACCAGCGCA AGGCCAAGCT GGGCGACTCC CACGACCTGC AGCGCTTCCT CAGCGACTTC 
CGGGACCTCA TGTCTTGGAT CAATGGAATC CGAGGTCTGG TGTCCTCAGA CGAGCTAGCC 
AAGGATGTCA CTGGAGCTGA GGCCCTGCTG GAGCGACACC AGGAACACCG GACAGAAATT 
GATGCCAGAG CTGGCACTTT CCAGGCATTC GAGCAGTTTG GGCAGCAGCT GTTGGCTCAT 
GGGCACTACG CCAGCCCCGA GATCAAGGAG AAGCTCGATG TTCTAGACCA GGAGCGCGCA 
GACCTCGAGA AGGCCTGGGT CCAGCGCAGG ATGATGCTGG ATCAGTGCCT TGAATTGCAG 
CTGTTCCATC GGGATTGCGA GCAGGCCGAG AACTGGATGG CTGCCCGGGA GGCCTTCCTG 
AACACAGAAG ACAAAGGGGA CTCGCTAGAC AGCGTGGAGG CTCTGATCAA AAAACACGAG 
GACTTTGACA AAGCAATTAA TGTCCAGGAG GAGAAGATTG CTGCCCTGCA GTCCTTTGCT 
GACCAGCTCA TTGCTGGCGG TCACTACGCC AAAGGCGACA TCTCCAGCCG GCGCAATGAG 
GTTCTGGACA GGTGGCGCCG TCTAAAAGCT CAGATGATTG AGAAGAGGTC AAAGCTGGGA 
GAATCGCAGA CCCTCCAGCA GTTCAGCCGG GATGTAGACG AGATTGAAGC TTGGATCAGT 
GAAAAATTAC AGACTGCAAG TGATGAGTCA TATAAGGACC CCACCAACAT CCAGCTTTCC 
AAGCTGCTGA GCAAGCATCA GAAGCACCAG GCCTTCGAGG CAGAACTGCA CGCCAATGCG 
GACCGGATCC GCGGGGTCAT CGACGTGGGC AACTCCCTCA TTGACCGTGG GGCCTGTGCC 
GGCAGCGAGG ATGCCGTCAA GGCCCGGCTG GCCGCCTTAG CTGACCAGTG GCAGTTCCTG 
GTGCAGAAGT CGGCTGAGAA GAGCCAGAAG CTGAAAGAAG CCAACAAACA GCAGAACTTC 
AACACTGGCA TCAAGGACTT CGACTTCTGG CTCTCTGAGG TGGAAGCCCT GCTGGCCTCT 
GAGGATTATG GCAAAGACCT GGCTTCTGTG AACAACTTGC TAAAAAAACA TCAGCTGCTG 
GAGGCAGATA TCTCTGCCCA CGAGGACCGC CTGAAGGACC TCAACAGCCA GGCGGACAGC 
CTGATGACCA GCAGCGCCTT CGACACCTCC CAGGTGAAGG ATAAGCGGGA CACCATCAAC 
AGCCGTTTCC AGAAGATCAA GAGCATGGCC GCCTCCCGGC GAGCTAGGCT GAATGAGTCC 
CACCGCCTGC ACCAGTTCTT CCGGGACATG GACGATGAGG AGTCCTGGAT CAAGGAGAAG 
AAGCTGCTGG TGAGCTCAGA GGACTACGGC CGCGACCTGA CGGGCGTCCA GAACCTGAGG 
AAGAAGCACA AACGGCTGGA GGCAGAGCTG GCTGCCCACG AGCCGGCCAT TCAGGGTGTC 
CTGGACACTG GAAAGAAGCT GTCTGATGAC AACACCATCG GGAAGGAGGA GATTCAGCAG 
CGCCTGGCGC AGTTTGTGGA GCACTGGAAG GAGCTGAAGC AGCTGGCAGC TGCTCGGGGC 
CAGCGGCTAG AAGAATCTTT GGAATATCAG CAGTTTGTGG CCAACGTGGA GGAGGAGGAA 
GCCTGGATCA ATGAGAAGAT GACCCTGGTG GCCAGTGAGG ATTACGGAGA CACTCTTGCT 
GCCATCCAGG GCTTGCTGAA GAAACACGAA GCCTTCGAGA CGGACTTCAC TGTCCACAAG 
GACCGGGTGA ATGACGTCTG CACTAACGGA CAAGACCTGG TTAAGAAGAA CAATCACCAT 
GAGGAGAACA TCTCTTCCAA GATGAAGGGC TTGAACGGGA AAGTGTCAGA CTTGGAGAAG 
GCTGCCTCCC AGAGGAAGGC AAAGCTCGAC GAGAACTCGG CCTTCCTCCA GTTCAACTGG 
AAGGCCGATG TGGTGGAGTC CTGGATTGGT GAAAAGGAGA ACAGCTTGAA GACAGATGAT 
TATGGTCGAG ATCTGTCTTC TGTCCAAACT CTCCTCACCA AACAGGAAAC CTTCGATGCT 
GGCCTGCAGG CCTTCCAGCA GGAGGGCATC GCCAACATCA CTGCCCTCAA AGATCAGCTG 
CTGGCCGCCA AGCATGTTCA GTCCAAGGCC ATCGAGGCCC GCCACGCTGC CCTCATGAAG 
AGATGGAGCC AGCTCCTGGC CAATTCGGCC ACCCGGAAGA AGAAGCTGCT GGAGGCACAG 
AGTCACTTCC GCAAGGTGGA GGATCTCTTC CTGACCTTTG CCAAAAAGGC TTCAGCCTTC 
AACAGCTGGT TTGAAAATGC AGAGGAGGAC TTAACAGACC CAGTGCGCTG CAACTCCCTG 
GAAGAGATCA AGGCCTTGAG AGAGGCCCAC GATGCCTTCC GCTCCTCGCT GAGCTCAGCC 
CAGGCCGACT TCAACCAGCT GGCTGAGCTG GACCGCCAGA TCAAGAGCTT CCGCGTGGCC 
TCCAACCCCT ACACCTGGTT CACCATGGAG GCCCTGGAGG AGACCTGGAG GAACCTTCAG 
AAGATCATCA AGGAAAGGGA GCTGGAGCTG CAGAAGGAGC AGCGGCGGCA GGAGGAGAAC 
GACAAGCTGC GCCAGGAGTT CGCCCAGCAT GCCAACGCCT TCCACCAGTG GATCCAGGAG 
ACCAGAACGT ATCTCCTCGA TGGCATAGCG TATCGTCGGG TCATTCGTGT CTATCAGTAT 
GAAGTTGGGG ATGATCTGTC TGGAAGGTCC TGCATGGTGG AAGAATCTGG GACCCTTGAA 
TCGCAGCTTG AAGCTACCAA ACGCAAACAC CAGGAGATTC GAGCCATGAG AAGTCAGCTC 
AAGAAGATTG AAGACCTGGG GGCGGCCATG GAGGAAGCGC TGATCCTGGA CAACAAGTAC 
ACGGAGCACA GCACCGTGGG CCTGGCCCAG CAGTGGGACC AGCTCGACCA GCTGGGCATG 
CGGATGCAGC ACAACCTGGA GCAGCAGATC CAGGCCAGGA ACACGACAGG AGTGACCGAG 
GAGGCCCTCA AAGAATTCAG CATGATGTTC AAACACTTCG ACAAGGACAA GTCCGGCCGG 
CTGAATCACC AGGAGTTCAA ATCCTGTCTG CGCTCCCTGG GCTATGACCT GCCCATGGTG 
GAGGAAGGGG AGCCCGACCC TGAGTTCGAG GCCATCTTGG ATACTGTGGA TCCTAACAGG 
GACGGCCACG TCTCCCTGCA AGAGTACATG GCCTTTATGA TCAGCCGTGA GACCGAGAAT 
GTCAAGTCCA GCGAGGAGAT TGAGAGCGCC TTCCGGGCCC TGAGCTCCGA AGGGAAGCCT 
TATGTGACCA AGGAGGAGCT GTACCAGAAC CTGACTCGGG AACAAGCCGA CTACTGCGTC 
TCCCACATGA AGCCCTACGT GGACGGCAAG GGCCGCGAAC TCCCCACTGC TTTCGACTAC 
GTTGAGTTCA CCCGCTCGCT CTTCGTGAAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012002867.1
CDS	82..7614
Translation	MLSSFRKRRVQKMDPSGVKVLETAEDIQERRQQVLDRYHRFKELSTLRRQKLEDSYRFQFFQRDAEELEKWIQEKLQIASDENYKDPTNLQGKLQKHQAFEAEVQANSGAIVKLDETGNLMISEGHFASETIRTRLMELHRQWELLLEKMREKGIKLLQAQKLVQYLRECEDVMDWINDKEAIVTSEELGQDLEHVEVLQKKFEEFQTDMAAHEERVNEVNQFAAKLIQEQHPEEELIKTKEDEVNAAWQRLKGLALQRQGKLFGAAEVQRFNRDVDETISWIKEKEQLMASDDFGRDLASVQALLRKHEGLERDLAALEDKVKALCAEADRLQQSHPLSATQIQVKREELITNWEQIRTLAAERHARLNDSYRLQRFLADFRDLTSWVTEMKALINADELANDVAGAEALLDRHQEHKGEIDAHEDSFKSADESGQALLAAGHYASDEVREKLTILSEERAALLELWELRRQQYEQCMDLQLFYRDTEQVDNWMSKQEAFLLNEDLGDSLDSVEALLKKHEDFEKSLSAQEEKITALDEFATKLIQNNHYAMEDVATRRDALLSRRNALHERAMYRRAQLADSFHLQQFFRDSDELKSWVNEKMKTATDEAYKDPSNLQGKVQKHQAFEAELSANQSRIDALEKAGQKLIDVDHYAKDEVATRMNEVISLWKKLLEATELKGIKLREANQQQQFNRNVEDIELWLYEVEGHLASDDYGKDLTNVQNLQKKHALLEADVAAHQDRIDGITIQARQFQDAGHFDAENIKKKQEALVARYEALKEPMVARKQKLADSLRLQQLFRDVEDEETWIREKEPIAASTNRGKDLIGVQNLLKKHQALQAEIAGHEPRIKAVTQKGNAMVEEGHFAAEDVKAKLHELNQKWESLKSKASQRRQDLEDSLQAQQYFADANEAESWMREKEPIVGSTDYGKDEDSAEALLKKHEALMSDLSAYGSSIQALREQAQSCRQQVAPMDDETGKELVLALYDYQEKSPREVTMKKGDILTLLNSTNKDWWKVEVNDRQGFVPAAYVKKLDPAQSASRENLLEEQGSIALRQEQIDNQTRVTKEAGSVSLRMKQVEELYHSLLELGEKRKGMLEKSCKKFMLFREANELQQWINEKEAALTSEEVGADLEQVEVLQKKFDDFQKDLKANESRLKDINKVAEDLESEGLMAEEVQAVQQQEVYGAMPRDESDSKTASPWKSARLMVHTVATFNSIKELNERWRSLQQLAEERSQLLGSAHEVQRFHRDADETKEWIEEKNQALNTDNYGHDLASVQALQRKHEGFERDLAALGDKVNSLGETAERLIQSHPESAEDLQEKCTELNQAWSSLGKRADQRKAKLGDSHDLQRFLSDFRDLMSWINGIRGLVSSDELAKDVTGAEALLERHQEHRTEIDARAGTFQAFEQFGQQLLAHGHYASPEIKEKLDVLDQERADLEKAWVQRRMMLDQCLELQLFHRDCEQAENWMAAREAFLNTEDKGDSLDSVEALIKKHEDFDKAINVQEEKIAALQSFADQLIAGGHYAKGDISSRRNEVLDRWRRLKAQMIEKRSKLGESQTLQQFSRDVDEIEAWISEKLQTASDESYKDPTNIQLSKLLSKHQKHQAFEAELHANADRIRGVIDVGNSLIDRGACAGSEDAVKARLAALADQWQFLVQKSAEKSQKLKEANKQQNFNTGIKDFDFWLSEVEALLASEDYGKDLASVNNLLKKHQLLEADISAHEDRLKDLNSQADSLMTSSAFDTSQVKDKRDTINSRFQKIKSMAASRRARLNESHRLHQFFRDMDDEESWIKEKKLLVSSEDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQFVEHWKELKQLAAARGQRLEESLEYQQFVANVEEEEAWINEKMTLVASEDYGDTLAAIQGLLKKHEAFETDFTVHKDRVNDVCTNGQDLVKKNNHHEENISSKMKGLNGKVSDLEKAASQRKAKLDENSAFLQFNWKADVVESWIGEKENSLKTDDYGRDLSSVQTLLTKQETFDAGLQAFQQEGIANITALKDQLLAAKHVQSKAIEARHAALMKRWSQLLANSATRKKKLLEAQSHFRKVEDLFLTFAKKASAFNSWFENAEEDLTDPVRCNSLEEIKALREAHDAFRSSLSSAQADFNQLAELDRQIKSFRVASNPYTWFTMEALEETWRNLQKIIKERELELQKEQRRQEENDKLRQEFAQHANAFHQWIQETRTYLLDGIAYRRVIRVYQYEVGDDLSGRSCMVEESGTLESQLEATKRKHQEIRAMRSQLKKIEDLGAAMEEALILDNKYTEHSTVGLAQQWDQLDQLGMRMQHNLEQQIQARNTTGVTEEALKEFSMMFKHFDKDKSGRLNHQEFKSCLRSLGYDLPMVEEGEPDPEFEAILDTVDPNRDGHVSLQEYMAFMISRETENVKSSEEIESAFRALSSEGKPYVTKEELYQNLTREQADYCVSHMKPYVDGKGRELPTAFDYVEFTRSLFVN

Target ORF information:

RefSeq Version	XM_012147477.1
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon spectrin, alpha, non-erythrocytic 1 (SPTAN1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012147477.1

ORF Insert Sequence:

ATGCTGTCAT CGTTTCGTAA ACGCAGAGTC CAGAAAATGG ACCCAAGCGG GGTCAAAGTG 
CTGGAGACAG CCGAGGACAT CCAGGAGAGG CGGCAGCAGG TCCTGGACAG ATACCACCGC 
TTCAAGGAGC TCTCGACCCT GAGGCGCCAG AAGCTTGAAG ACTCCTACCG ATTCCAGTTT 
TTCCAAAGAG ATGCTGAGGA GCTGGAGAAA TGGATTCAGG AGAAGCTTCA GATTGCCTCG 
GATGAGAATT ACAAAGACCC AACCAACCTG CAGGGCAAGC TGCAGAAGCA TCAAGCCTTC 
GAAGCCGAGG TGCAGGCCAA CTCGGGTGCC ATCGTGAAGC TTGATGAGAC CGGTAACCTG 
ATGATCTCAG AAGGGCACTT CGCGTCCGAA ACCATCCGGA CTCGTCTGAT GGAGCTGCAC 
CGCCAGTGGG AATTGCTTCT GGAGAAGATG CGGGAGAAAG GCATCAAGCT GCTGCAGGCC 
CAGAAGCTGG TGCAGTACCT GCGGGAGTGT GAGGACGTGA TGGACTGGAT CAATGACAAG 
GAAGCCATTG TGACATCTGA GGAGCTGGGC CAGGACCTGG AGCACGTGGA GGTGTTACAG 
AAGAAATTCG AAGAGTTTCA GACAGACATG GCTGCCCACG AAGAGAGAGT CAATGAAGTG 
AACCAGTTTG CTGCCAAGCT CATTCAGGAG CAGCACCCTG AGGAGGAGCT GATCAAGACA 
AAGGAGGACG AGGTGAATGC AGCCTGGCAG CGGCTGAAGG GCCTGGCCCT CCAGCGGCAG 
GGGAAGCTGT TCGGGGCAGC TGAGGTCCAA CGCTTTAACA GGGATGTGGA CGAGACCATT 
AGCTGGATTA AGGAGAAGGA ACAGTTAATG GCCTCTGATG ACTTTGGCCG GGACCTGGCC 
AGTGTCCAGG CTCTACTGCG GAAACACGAG GGTTTGGAGA GAGATCTTGC TGCCTTGGAG 
GACAAGGTCA AAGCCCTGTG TGCCGAGGCT GACCGCCTGC AGCAGTCCCA CCCTCTGAGT 
GCCACCCAGA TCCAAGTGAA GCGAGAGGAG CTGATCACCA ACTGGGAGCA GATCCGCACC 
CTGGCCGCGG AGAGACACGC ACGTCTCAAC GATTCCTACA GGCTTCAGCG CTTCCTTGCT 
GACTTCCGAG ACCTCACCAG CTGGGTCACT GAGATGAAAG CCCTCATCAA TGCAGATGAA 
CTTGCCAATG ATGTGGCCGG GGCCGAAGCC CTGCTGGATA GGCATCAGGA GCACAAGGGT 
GAAATTGACG CTCATGAAGA TAGCTTTAAA TCTGCAGATG AGTCTGGACA GGCCCTGCTT 
GCAGCTGGTC ACTATGCCTC AGATGAAGTG AGGGAGAAGC TGACCATCCT GTCCGAGGAG 
CGGGCCGCGC TACTAGAGCT CTGGGAGCTG CGACGGCAGC AATATGAGCA GTGCATGGAC 
CTGCAGCTCT TCTACCGCGA CACGGAGCAG GTGGACAACT GGATGAGCAA GCAGGAGGCG 
TTCCTGTTGA ACGAAGACCT GGGTGATTCC TTGGATAGTG TGGAAGCGCT TCTCAAGAAG 
CACGAAGACT TTGAGAAGTC CCTCAGCGCC CAGGAGGAGA AGATCACAGC CTTAGATGAG 
TTTGCCACCA AGCTGATTCA GAACAACCAC TACGCGATGG AAGATGTGGC CACCCGGCGA 
GACGCACTGC TGAGCCGCCG CAACGCCCTC CATGAGAGAG CCATGTACCG CCGTGCCCAG 
CTTGCAGATT CCTTCCACTT GCAGCAGTTT TTCCGTGACT CTGATGAGCT CAAGAGTTGG 
GTCAACGAGA AGATGAAAAC AGCCACAGAT GAAGCTTATA AAGACCCATC CAACCTACAA 
GGGAAAGTGC AGAAGCACCA GGCCTTTGAG GCCGAGCTCT CAGCAAACCA GAGCCGCATC 
GACGCCTTGG AGAAAGCTGG GCAGAAGCTG ATCGACGTTG ACCACTATGC CAAGGATGAG 
GTGGCGACTC GGATGAATGA AGTTATCAGT CTGTGGAAGA AGCTGCTGGA GGCCACGGAG 
CTGAAAGGCA TAAAGCTCCG TGAGGCTAAC CAGCAGCAGC AGTTTAACCG CAATGTTGAG 
GACATCGAGC TGTGGCTGTA CGAAGTGGAA GGTCATCTGG CCTCGGATGA CTACGGCAAA 
GACCTCACCA ACGTCCAGAA CCTCCAGAAA AAGCATGCCC TGCTGGAGGC TGACGTGGCC 
GCTCACCAGG ATCGCATCGA TGGCATCACT ATCCAAGCCC GCCAGTTCCA GGATGCGGGC 
CACTTTGATG CTGAAAACAT CAAGAAGAAG CAGGAGGCCC TGGTAGCTCG CTACGAGGCA 
CTCAAGGAGC CCATGGTTGC CCGGAAGCAG AAGCTGGCCG ATTCCCTGCG GTTGCAGCAG 
CTCTTCCGTG ACGTTGAGGA TGAGGAGACG TGGATTCGGG AGAAAGAACC TATTGCCGCC 
TCCACCAACA GAGGCAAAGA TTTAATTGGA GTCCAGAACC TGCTAAAGAA ACACCAAGCG 
CTACAAGCAG AAATTGCTGG CCACGAGCCC CGCATCAAAG CAGTCACACA GAAGGGGAAC 
GCCATGGTAG AGGAGGGCCA TTTTGCTGCC GAGGACGTGA AGGCCAAGCT CCATGAACTG 
AACCAGAAGT GGGAGTCGCT GAAGTCCAAG GCCTCCCAGC GACGGCAGGA CCTGGAGGAC 
TCGCTGCAGG CCCAGCAGTA CTTCGCTGAT GCCAACGAGG CCGAGTCCTG GATGCGGGAG 
AAGGAGCCCA TCGTGGGCAG CACCGACTAT GGCAAAGATG AGGACTCTGC CGAGGCTCTG 
CTAAAGAAGC ACGAGGCATT GATGTCGGAT CTCAGCGCCT ATGGCAGCAG CATCCAGGCC 
CTGCGAGAGC AGGCCCAGTC GTGCCGGCAA CAAGTGGCCC CCATGGATGA CGAGACTGGG 
AAGGAGCTGG TCTTGGCGCT CTATGACTAT CAGGAGAAGA GTCCCCGAGA GGTCACCATG 
AAGAAAGGAG ACATTCTCAC CTTACTCAAC AGCACCAACA AGGACTGGTG GAAAGTGGAA 
GTGAACGACC GGCAGGGCTT CGTGCCGGCC GCCTACGTGA AGAAGCTGGA CCCAGCGCAG 
TCGGCCTCCC GGGAGAACCT CCTGGAAGAG CAGGGCAGCA TCGCGCTGCG GCAGGAGCAG 
ATTGACAACC AGACGCGCGT AACTAAGGAG GCCGGCAGCG TGTCTCTGCG TATGAAACAG 
GTGGAAGAAC TGTACCATTC TCTGCTGGAA CTGGGAGAGA AGCGTAAAGG CATGTTGGAG 
AAGAGCTGTA AGAAGTTCAT GCTGTTCCGT GAAGCGAACG AGCTGCAGCA GTGGATCAAC 
GAGAAGGAAG CGGCCCTGAC AAGCGAGGAG GTCGGCGCGG ACTTGGAGCA GGTCGAAGTG 
CTGCAGAAGA AGTTTGACGA CTTCCAGAAG GATCTCAAGG CCAATGAGTC ACGGCTGAAG 
GACATTAACA AGGTGGCTGA AGACCTGGAG TCGGAAGGCC TGATGGCGGA GGAGGTGCAG 
GCCGTGCAGC AGCAGGAGGT GTATGGCGCG ATGCCCAGGG ATGAATCTGA TTCCAAGACA 
GCCTCCCCGT GGAAGTCTGC TCGCCTGATG GTTCACACCG TGGCCACCTT TAATTCCATC 
AAGGAGCTCA ATGAGCGCTG GCGGTCCCTG CAGCAGCTGG CTGAGGAGCG GAGCCAACTC 
TTGGGCAGCG CCCACGAAGT GCAGCGGTTC CACAGAGATG CTGACGAAAC CAAAGAGTGG 
ATCGAGGAGA AGAACCAGGC TCTAAACACA GACAACTATG GCCACGACCT GGCCAGCGTC 
CAGGCCCTGC AGCGCAAGCA TGAGGGCTTC GAGAGGGACC TCGCGGCCCT TGGCGACAAG 
GTGAACTCGC TCGGGGAGAC AGCCGAGCGC CTGATCCAGT CCCACCCCGA GTCGGCAGAG 
GACCTGCAGG AGAAGTGCAC TGAGCTGAAC CAGGCATGGA GCAGCCTGGG CAAGCGTGCC 
GACCAGCGCA AGGCCAAGCT GGGCGACTCC CACGACCTGC AGCGCTTCCT CAGCGACTTC 
CGGGACCTCA TGTCTTGGAT CAATGGAATC CGAGGTCTGG TGTCCTCAGA CGAGCTAGCC 
AAGGATGTCA CTGGAGCTGA GGCCCTGCTG GAGCGACACC AGGAACACCG GACAGAAATT 
GATGCCAGAG CTGGCACTTT CCAGGCATTC GAGCAGTTTG GGCAGCAGCT GTTGGCTCAT 
GGGCACTACG CCAGCCCCGA GATCAAGGAG AAGCTCGATG TTCTAGACCA GGAGCGCGCA 
GACCTCGAGA AGGCCTGGGT CCAGCGCAGG ATGATGCTGG ATCAGTGCCT TGAATTGCAG 
CTGTTCCATC GGGATTGCGA GCAGGCCGAG AACTGGATGG CTGCCCGGGA GGCCTTCCTG 
AACACAGAAG ACAAAGGGGA CTCGCTAGAC AGCGTGGAGG CTCTGATCAA AAAACACGAG 
GACTTTGACA AAGCAATTAA TGTCCAGGAG GAGAAGATTG CTGCCCTGCA GTCCTTTGCT 
GACCAGCTCA TTGCTGGCGG TCACTACGCC AAAGGCGACA TCTCCAGCCG GCGCAATGAG 
GTTCTGGACA GGTGGCGCCG TCTAAAAGCT CAGATGATTG AGAAGAGGTC AAAGCTGGGA 
GAATCGCAGA CCCTCCAGCA GTTCAGCCGG GATGTAGACG AGATTGAAGC TTGGATCAGT 
GAAAAATTAC AGACTGCAAG TGATGAGTCA TATAAGGACC CCACCAACAT CCAGCTTTCC 
AAGCTGCTGA GCAAGCATCA GAAGCACCAG GCCTTCGAGG CAGAACTGCA CGCCAATGCG 
GACCGGATCC GCGGGGTCAT CGACGTGGGC AACTCCCTCA TTGACCGTGG GGCCTGTGCC 
GGCAGCGAGG ATGCCGTCAA GGCCCGGCTG GCCGCCTTAG CTGACCAGTG GCAGTTCCTG 
GTGCAGAAGT CGGCTGAGAA GAGCCAGAAG CTGAAAGAAG CCAACAAACA GCAGAACTTC 
AACACTGGCA TCAAGGACTT CGACTTCTGG CTCTCTGAGG TGGAAGCCCT GCTGGCCTCT 
GAGGATTATG GCAAAGACCT GGCTTCTGTG AACAACTTGC TAAAAAAACA TCAGCTGCTG 
GAGGCAGATA TCTCTGCCCA CGAGGACCGC CTGAAGGACC TCAACAGCCA GGCGGACAGC 
CTGATGACCA GCAGCGCCTT CGACACCTCC CAGGTGAAGG ATAAGCGGGA CACCATCAAC 
AGCCGTTTCC AGAAGATCAA GAGCATGGCC GCCTCCCGGC GAGCTAGGCT GAATGAGTCC 
CACCGCCTGC ACCAGTTCTT CCGGGACATG GACGATGAGG AGTCCTGGAT CAAGGAGAAG 
AAGCTGCTGG TGAGCTCAGA GGACTACGGC CGCGACCTGA CGGGCGTCCA GAACCTGAGG 
AAGAAGCACA AACGGCTGGA GGCAGAGCTG GCTGCCCACG AGCCGGCCAT TCAGGGTGTC 
CTGGACACTG GAAAGAAGCT GTCTGATGAC AACACCATCG GGAAGGAGGA GATTCAGCAG 
CGCCTGGCGC AGTTTGTGGA GCACTGGAAG GAGCTGAAGC AGCTGGCAGC TGCTCGGGGC 
CAGCGGCTAG AAGAATCTTT GGAATATCAG CAGTTTGTGG CCAACGTGGA GGAGGAGGAA 
GCCTGGATCA ATGAGAAGAT GACCCTGGTG GCCAGTGAGG ATTACGGAGA CACTCTTGCT 
GCCATCCAGG GCTTGCTGAA GAAACACGAA GCCTTCGAGA CGGACTTCAC TGTCCACAAG 
GACCGGGTGA ATGACGTCTG CACTAACGGA CAAGACCTGG TTAAGAAGAA CAATCACCAT 
GAGGAGAACA TCTCTTCCAA GATGAAGGGC TTGAACGGGA AAGTGTCAGA CTTGGAGAAG 
GCTGCCTCCC AGAGGAAGGC AAAGCTCGAC GAGAACTCGG CCTTCCTCCA GTTCAACTGG 
AAGGCCGATG TGGTGGAGTC CTGGATTGGT GAAAAGGAGA ACAGCTTGAA GACAGATGAT 
TATGGTCGAG ATCTGTCTTC TGTCCAAACT CTCCTCACCA AACAGGAAAC CTTCGATGCT 
GGCCTGCAGG CCTTCCAGCA GGAGGGCATC GCCAACATCA CTGCCCTCAA AGATCAGCTG 
CTGGCCGCCA AGCATGTTCA GTCCAAGGCC ATCGAGGCCC GCCACGCTGC CCTCATGAAG 
AGATGGAGCC AGCTCCTGGC CAATTCGGCC ACCCGGAAGA AGAAGCTGCT GGAGGCACAG 
AGTCACTTCC GCAAGGTGGA GGATCTCTTC CTGACCTTTG CCAAAAAGGC TTCAGCCTTC 
AACAGCTGGT TTGAAAATGC AGAGGAGGAC TTAACAGACC CAGTGCGCTG CAACTCCCTG 
GAAGAGATCA AGGCCTTGAG AGAGGCCCAC GATGCCTTCC GCTCCTCGCT GAGCTCAGCC 
CAGGCCGACT TCAACCAGCT GGCTGAGCTG GACCGCCAGA TCAAGAGCTT CCGCGTGGCC 
TCCAACCCCT ACACCTGGTT CACCATGGAG GCCCTGGAGG AGACCTGGAG GAACCTTCAG 
AAGATCATCA AGGAAAGGGA GCTGGAGCTG CAGAAGGAGC AGCGGCGGCA GGAGGAGAAC 
GACAAGCTGC GCCAGGAGTT CGCCCAGCAT GCCAACGCCT TCCACCAGTG GATCCAGGAG 
ACCAGAACGT ATCTCCTCGA TGGCATAGCG TATCGTCGGG TCATTCGTGT CTATCAGTAT 
GAAGTTGGGG ATGATCTGTC TGGAAGGTCC TGCATGGTGG AAGAATCTGG GACCCTTGAA 
TCGCAGCTTG AAGCTACCAA ACGCAAACAC CAGGAGATTC GAGCCATGAG AAGTCAGCTC 
AAGAAGATTG AAGACCTGGG GGCGGCCATG GAGGAAGCGC TGATCCTGGA CAACAAGTAC 
ACGGAGCACA GCACCGTGGG CCTGGCCCAG CAGTGGGACC AGCTCGACCA GCTGGGCATG 
CGGATGCAGC ACAACCTGGA GCAGCAGATC CAGGCCAGGA ACACGACAGG AGTGACCGAG 
GAGGCCCTCA AAGAATTCAG CATGATGTTC AAACACTTCG ACAAGGACAA GTCCGGCCGG 
CTGAATCACC AGGAGTTCAA ATCCTGTCTG CGCTCCCTGG GCTATGACCT GCCCATGGTG 
GAGGAAGGGG AGCCCGACCC TGAGTTCGAG GCCATCTTGG ATACTGTGGA TCCTAACAGG 
GACGGCCACG TCTCCCTGCA AGAGTACATG GCCTTTATGA TCAGCCGTGA GACCGAGAAT 
GTCAAGTCCA GCGAGGAGAT TGAGAGCGCC TTCCGGGCCC TGAGCTCCGA AGGGAAGCCT 
TATGTGACCA AGGAGGAGCT GTACCAGAAC CTGACTCGGG AACAAGCCGA CTACTGCGTC 
TCCCACATGA AGCCCTACGT GGACGGCAAG GGCCGCGAAC TCCCCACTGC TTTCGACTAC 
GTTGAGTTCA CCCGCTCGCT CTTCGTGAAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTAN1 ( XM_012147478.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_012002868.1 Ovis aries musimon spectrin, alpha, non-erythrocytic 1 (SPTAN1), transcript variant X3, mRNA.

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CloneID	OOv24450
Clone ID Related Accession (Same CDS sequence)	XM_012147478.1
Accession Version	XM_012147478.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7518bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	spectrin alpha chain, non-erythrocytic 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942583.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTCAT CGTTTCGTAA ACGCAGAGTC CAGAAAATGG ACCCAAGCGG GGTCAAAGTG 
CTGGAGACAG CCGAGGACAT CCAGGAGAGG CGGCAGCAGG TCCTGGACAG ATACCACCGC 
TTCAAGGAGC TCTCGACCCT GAGGCGCCAG AAGCTTGAAG ACTCCTACCG ATTCCAGTTT 
TTCCAAAGAG ATGCTGAGGA GCTGGAGAAA TGGATTCAGG AGAAGCTTCA GATTGCCTCG 
GATGAGAATT ACAAAGACCC AACCAACCTG CAGGGCAAGC TGCAGAAGCA TCAAGCCTTC 
GAAGCCGAGG TGCAGGCCAA CTCGGGTGCC ATCGTGAAGC TTGATGAGAC CGGTAACCTG 
ATGATCTCAG AAGGGCACTT CGCGTCCGAA ACCATCCGGA CTCGTCTGAT GGAGCTGCAC 
CGCCAGTGGG AATTGCTTCT GGAGAAGATG CGGGAGAAAG GCATCAAGCT GCTGCAGGCC 
CAGAAGCTGG TGCAGTACCT GCGGGAGTGT GAGGACGTGA TGGACTGGAT CAATGACAAG 
GAAGCCATTG TGACATCTGA GGAGCTGGGC CAGGACCTGG AGCACGTGGA GGTGTTACAG 
AAGAAATTCG AAGAGTTTCA GACAGACATG GCTGCCCACG AAGAGAGAGT CAATGAAGTG 
AACCAGTTTG CTGCCAAGCT CATTCAGGAG CAGCACCCTG AGGAGGAGCT GATCAAGACA 
AAGGAGGACG AGGTGAATGC AGCCTGGCAG CGGCTGAAGG GCCTGGCCCT CCAGCGGCAG 
GGGAAGCTGT TCGGGGCAGC TGAGGTCCAA CGCTTTAACA GGGATGTGGA CGAGACCATT 
AGCTGGATTA AGGAGAAGGA ACAGTTAATG GCCTCTGATG ACTTTGGCCG GGACCTGGCC 
AGTGTCCAGG CTCTACTGCG GAAACACGAG GGTTTGGAGA GAGATCTTGC TGCCTTGGAG 
GACAAGGTCA AAGCCCTGTG TGCCGAGGCT GACCGCCTGC AGCAGTCCCA CCCTCTGAGT 
GCCACCCAGA TCCAAGTGAA GCGAGAGGAG CTGATCACCA ACTGGGAGCA GATCCGCACC 
CTGGCCGCGG AGAGACACGC ACGTCTCAAC GATTCCTACA GGCTTCAGCG CTTCCTTGCT 
GACTTCCGAG ACCTCACCAG CTGGGTCACT GAGATGAAAG CCCTCATCAA TGCAGATGAA 
CTTGCCAATG ATGTGGCCGG GGCCGAAGCC CTGCTGGATA GGCATCAGGA GCACAAGGGT 
GAAATTGACG CTCATGAAGA TAGCTTTAAA TCTGCAGATG AGTCTGGACA GGCCCTGCTT 
GCAGCTGGTC ACTATGCCTC AGATGAAGTG AGGGAGAAGC TGACCATCCT GTCCGAGGAG 
CGGGCCGCGC TACTAGAGCT CTGGGAGCTG CGACGGCAGC AATATGAGCA GTGCATGGAC 
CTGCAGCTCT TCTACCGCGA CACGGAGCAG GTGGACAACT GGATGAGCAA GCAGGAGGCG 
TTCCTGTTGA ACGAAGACCT GGGTGATTCC TTGGATAGTG TGGAAGCGCT TCTCAAGAAG 
CACGAAGACT TTGAGAAGTC CCTCAGCGCC CAGGAGGAGA AGATCACAGC CTTAGATGAG 
TTTGCCACCA AGCTGATTCA GAACAACCAC TACGCGATGG AAGATGTGGC CACCCGGCGA 
GACGCACTGC TGAGCCGCCG CAACGCCCTC CATGAGAGAG CCATGTACCG CCGTGCCCAG 
CTTGCAGATT CCTTCCACTT GCAGCAGTTT TTCCGTGACT CTGATGAGCT CAAGAGTTGG 
GTCAACGAGA AGATGAAAAC AGCCACAGAT GAAGCTTATA AAGACCCATC CAACCTACAA 
GGGAAAGTGC AGAAGCACCA GGCCTTTGAG GCCGAGCTCT CAGCAAACCA GAGCCGCATC 
GACGCCTTGG AGAAAGCTGG GCAGAAGCTG ATCGACGTTG ACCACTATGC CAAGGATGAG 
GTGGCGACTC GGATGAATGA AGTTATCAGT CTGTGGAAGA AGCTGCTGGA GGCCACGGAG 
CTGAAAGGCA TAAAGCTCCG TGAGGCTAAC CAGCAGCAGC AGTTTAACCG CAATGTTGAG 
GACATCGAGC TGTGGCTGTA CGAAGTGGAA GGTCATCTGG CCTCGGATGA CTACGGCAAA 
GACCTCACCA ACGTCCAGAA CCTCCAGAAA AAGCATGCCC TGCTGGAGGC TGACGTGGCC 
GCTCACCAGG ATCGCATCGA TGGCATCACT ATCCAAGCCC GCCAGTTCCA GGATGCGGGC 
CACTTTGATG CTGAAAACAT CAAGAAGAAG CAGGAGGCCC TGGTAGCTCG CTACGAGGCA 
CTCAAGGAGC CCATGGTTGC CCGGAAGCAG AAGCTGGCCG ATTCCCTGCG GTTGCAGCAG 
CTCTTCCGTG ACGTTGAGGA TGAGGAGACG TGGATTCGGG AGAAAGAACC TATTGCCGCC 
TCCACCAACA GAGGCAAAGA TTTAATTGGA GTCCAGAACC TGCTAAAGAA ACACCAAGCG 
CTACAAGCAG AAATTGCTGG CCACGAGCCC CGCATCAAAG CAGTCACACA GAAGGGGAAC 
GCCATGGTAG AGGAGGGCCA TTTTGCTGCC GAGGACGTGA AGGCCAAGCT CCATGAACTG 
AACCAGAAGT GGGAGTCGCT GAAGTCCAAG GCCTCCCAGC GACGGCAGGA CCTGGAGGAC 
TCGCTGCAGG CCCAGCAGTA CTTCGCTGAT GCCAACGAGG CCGAGTCCTG GATGCGGGAG 
AAGGAGCCCA TCGTGGGCAG CACCGACTAT GGCAAAGATG AGGACTCTGC CGAGGCTCTG 
CTAAAGAAGC ACGAGGCATT GATGTCGGAT CTCAGCGCCT ATGGCAGCAG CATCCAGGCC 
CTGCGAGAGC AGGCCCAGTC GTGCCGGCAA CAAGTGGCCC CCATGGATGA CGAGACTGGG 
AAGGAGCTGG TCTTGGCGCT CTATGACTAT CAGGAGAAGA GTCCCCGAGA GGTCACCATG 
AAGAAAGGAG ACATTCTCAC CTTACTCAAC AGCACCAACA AGGACTGGTG GAAAGTGGAA 
GTGAACGACC GGCAGGGCTT CGTGCCGGCC GCCTACGTGA AGAAGCTGGA CCCAGCGCAG 
TCGGCCTCCC GGGAGAACCT CCTGGAAGAG CAGGGCAGCA TCGCGCTGCG GCAGGAGCAG 
ATTGACAACC AGACGCGCGT AACTAAGGAG GCCGGCAGCG TGTCTCTGCG TATGAAACAG 
GTGGAAGAAC TGTACCATTC TCTGCTGGAA CTGGGAGAGA AGCGTAAAGG CATGTTGGAG 
AAGAGCTGTA AGAAGTTCAT GCTGTTCCGT GAAGCGAACG AGCTGCAGCA GTGGATCAAC 
GAGAAGGAAG CGGCCCTGAC AAGCGAGGAG GTCGGCGCGG ACTTGGAGCA GGTCGAAGTG 
CTGCAGAAGA AGTTTGACGA CTTCCAGAAG GATCTCAAGG CCAATGAGTC ACGGCTGAAG 
GACATTAACA AGGTGGCTGA AGACCTGGAG TCGGAAGGCC TGATGGCGGA GGAGGTGCAG 
GCCGTGCAGC AGCAGGAGGT GTATGGCGCG ATGCCCAGGG ATGAATCTGA TTCCAAGACA 
GCCTCCCCGT GGAAGTCTGC TCGCCTGATG GTTCACACCG TGGCCACCTT TAATTCCATC 
AAGGAGCTCA ATGAGCGCTG GCGGTCCCTG CAGCAGCTGG CTGAGGAGCG GAGCCAACTC 
TTGGGCAGCG CCCACGAAGT GCAGCGGTTC CACAGAGATG CTGACGAAAC CAAAGAGTGG 
ATCGAGGAGA AGAACCAGGC TCTAAACACA GACAACTATG GCCACGACCT GGCCAGCGTC 
CAGGCCCTGC AGCGCAAGCA TGAGGGCTTC GAGAGGGACC TCGCGGCCCT TGGCGACAAG 
GTGAACTCGC TCGGGGAGAC AGCCGAGCGC CTGATCCAGT CCCACCCCGA GTCGGCAGAG 
GACCTGCAGG AGAAGTGCAC TGAGCTGAAC CAGGCATGGA GCAGCCTGGG CAAGCGTGCC 
GACCAGCGCA AGGCCAAGCT GGGCGACTCC CACGACCTGC AGCGCTTCCT CAGCGACTTC 
CGGGACCTCA TGTCTTGGAT CAATGGAATC CGAGGTCTGG TGTCCTCAGA CGAGCTAGCC 
AAGGATGTCA CTGGAGCTGA GGCCCTGCTG GAGCGACACC AGGAACACCG GACAGAAATT 
GATGCCAGAG CTGGCACTTT CCAGGCATTC GAGCAGTTTG GGCAGCAGCT GTTGGCTCAT 
GGGCACTACG CCAGCCCCGA GATCAAGGAG AAGCTCGATG TTCTAGACCA GGAGCGCGCA 
GACCTCGAGA AGGCCTGGGT CCAGCGCAGG ATGATGCTGG ATCAGTGCCT TGAATTGCAG 
CTGTTCCATC GGGATTGCGA GCAGGCCGAG AACTGGATGG CTGCCCGGGA GGCCTTCCTG 
AACACAGAAG ACAAAGGGGA CTCGCTAGAC AGCGTGGAGG CTCTGATCAA AAAACACGAG 
GACTTTGACA AAGCAATTAA TGTCCAGGAG GAGAAGATTG CTGCCCTGCA GTCCTTTGCT 
GACCAGCTCA TTGCTGGCGG TCACTACGCC AAAGGCGACA TCTCCAGCCG GCGCAATGAG 
GTTCTGGACA GGTGGCGCCG TCTAAAAGCT CAGATGATTG AGAAGAGGTC AAAGCTGGGA 
GAATCGCAGA CCCTCCAGCA GTTCAGCCGG GATGTAGACG AGATTGAAGC TTGGATCAGT 
GAAAAATTAC AGACTGCAAG TGATGAGTCA TATAAGGACC CCACCAACAT CCAGAGCAAG 
CATCAGAAGC ACCAGGCCTT CGAGGCAGAA CTGCACGCCA ATGCGGACCG GATCCGCGGG 
GTCATCGACG TGGGCAACTC CCTCATTGAC CGTGGGGCCT GTGCCGGCAG CGAGGATGCC 
GTCAAGGCCC GGCTGGCCGC CTTAGCTGAC CAGTGGCAGT TCCTGGTGCA GAAGTCGGCT 
GAGAAGAGCC AGAAGCTGAA AGAAGCCAAC AAACAGCAGA ACTTCAACAC TGGCATCAAG 
GACTTCGACT TCTGGCTCTC TGAGGTGGAA GCCCTGCTGG CCTCTGAGGA TTATGGCAAA 
GACCTGGCTT CTGTGAACAA CTTGCTAAAA AAACATCAGC TGCTGGAGGC AGATATCTCT 
GCCCACGAGG ACCGCCTGAA GGACCTCAAC AGCCAGGCGG ACAGCCTGAT GACCAGCAGC 
GCCTTCGACA CCTCCCAGGT GAAGGATAAG CGGGACACCA TCAACAGCCG TTTCCAGAAG 
ATCAAGAGCA TGGCCGCCTC CCGGCGAGCT AGGCTGAATG AGTCCCACCG CCTGCACCAG 
TTCTTCCGGG ACATGGACGA TGAGGAGTCC TGGATCAAGG AGAAGAAGCT GCTGGTGAGC 
TCAGAGGACT ACGGCCGCGA CCTGACGGGC GTCCAGAACC TGAGGAAGAA GCACAAACGG 
CTGGAGGCAG AGCTGGCTGC CCACGAGCCG GCCATTCAGG GTGTCCTGGA CACTGGAAAG 
AAGCTGTCTG ATGACAACAC CATCGGGAAG GAGGAGATTC AGCAGCGCCT GGCGCAGTTT 
GTGGAGCACT GGAAGGAGCT GAAGCAGCTG GCAGCTGCTC GGGGCCAGCG GCTAGAAGAA 
TCTTTGGAAT ATCAGCAGTT TGTGGCCAAC GTGGAGGAGG AGGAAGCCTG GATCAATGAG 
AAGATGACCC TGGTGGCCAG TGAGGATTAC GGAGACACTC TTGCTGCCAT CCAGGGCTTG 
CTGAAGAAAC ACGAAGCCTT CGAGACGGAC TTCACTGTCC ACAAGGACCG GGTGAATGAC 
GTCTGCACTA ACGGACAAGA CCTGGTTAAG AAGAACAATC ACCATGAGGA GAACATCTCT 
TCCAAGATGA AGGGCTTGAA CGGGAAAGTG TCAGACTTGG AGAAGGCTGC CTCCCAGAGG 
AAGGCAAAGC TCGACGAGAA CTCGGCCTTC CTCCAGTTCA ACTGGAAGGC CGATGTGGTG 
GAGTCCTGGA TTGGTGAAAA GGAGAACAGC TTGAAGACAG ATGATTATGG TCGAGATCTG 
TCTTCTGTCC AAACTCTCCT CACCAAACAG GAAACCTTCG ATGCTGGCCT GCAGGCCTTC 
CAGCAGGAGG GCATCGCCAA CATCACTGCC CTCAAAGATC AGCTGCTGGC CGCCAAGCAT 
GTTCAGTCCA AGGCCATCGA GGCCCGCCAC GCTGCCCTCA TGAAGAGATG GAGCCAGCTC 
CTGGCCAATT CGGCCACCCG GAAGAAGAAG CTGCTGGAGG CACAGAGTCA CTTCCGCAAG 
GTGGAGGATC TCTTCCTGAC CTTTGCCAAA AAGGCTTCAG CCTTCAACAG CTGGTTTGAA 
AATGCAGAGG AGGACTTAAC AGACCCAGTG CGCTGCAACT CCCTGGAAGA GATCAAGGCC 
TTGAGAGAGG CCCACGATGC CTTCCGCTCC TCGCTGAGCT CAGCCCAGGC CGACTTCAAC 
CAGCTGGCTG AGCTGGACCG CCAGATCAAG AGCTTCCGCG TGGCCTCCAA CCCCTACACC 
TGGTTCACCA TGGAGGCCCT GGAGGAGACC TGGAGGAACC TTCAGAAGAT CATCAAGGAA 
AGGGAGCTGG AGCTGCAGAA GGAGCAGCGG CGGCAGGAGG AGAACGACAA GCTGCGCCAG 
GAGTTCGCCC AGCATGCCAA CGCCTTCCAC CAGTGGATCC AGGAGACCAG AACGTATCTC 
CTCGATGGCA TAGCGTATCG TCGGGTCATT CGTGTCTATC AGTATGAAGT TGGGGATGAT 
CTGTCTGGAA GGTCCTGCAT GGTGGAAGAA TCTGGGACCC TTGAATCGCA GCTTGAAGCT 
ACCAAACGCA AACACCAGGA GATTCGAGCC ATGAGAAGTC AGCTCAAGAA GATTGAAGAC 
CTGGGGGCGG CCATGGAGGA AGCGCTGATC CTGGACAACA AGTACACGGA GCACAGCACC 
GTGGGCCTGG CCCAGCAGTG GGACCAGCTC GACCAGCTGG GCATGCGGAT GCAGCACAAC 
CTGGAGCAGC AGATCCAGGC CAGGAACACG ACAGGAGTGA CCGAGGAGGC CCTCAAAGAA 
TTCAGCATGA TGTTCAAACA CTTCGACAAG GACAAGTCCG GCCGGCTGAA TCACCAGGAG 
TTCAAATCCT GTCTGCGCTC CCTGGGCTAT GACCTGCCCA TGGTGGAGGA AGGGGAGCCC 
GACCCTGAGT TCGAGGCCAT CTTGGATACT GTGGATCCTA ACAGGGACGG CCACGTCTCC 
CTGCAAGAGT ACATGGCCTT TATGATCAGC CGTGAGACCG AGAATGTCAA GTCCAGCGAG 
GAGATTGAGA GCGCCTTCCG GGCCCTGAGC TCCGAAGGGA AGCCTTATGT GACCAAGGAG 
GAGCTGTACC AGAACCTGAC TCGGGAACAA GCCGACTACT GCGTCTCCCA CATGAAGCCC 
TACGTGGACG GCAAGGGCCG CGAACTCCCC ACTGCTTTCG ACTACGTTGA GTTCACCCGC 
TCGCTCTTCG TGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012002868.1
CDS	82..7599
Translation	MLSSFRKRRVQKMDPSGVKVLETAEDIQERRQQVLDRYHRFKELSTLRRQKLEDSYRFQFFQRDAEELEKWIQEKLQIASDENYKDPTNLQGKLQKHQAFEAEVQANSGAIVKLDETGNLMISEGHFASETIRTRLMELHRQWELLLEKMREKGIKLLQAQKLVQYLRECEDVMDWINDKEAIVTSEELGQDLEHVEVLQKKFEEFQTDMAAHEERVNEVNQFAAKLIQEQHPEEELIKTKEDEVNAAWQRLKGLALQRQGKLFGAAEVQRFNRDVDETISWIKEKEQLMASDDFGRDLASVQALLRKHEGLERDLAALEDKVKALCAEADRLQQSHPLSATQIQVKREELITNWEQIRTLAAERHARLNDSYRLQRFLADFRDLTSWVTEMKALINADELANDVAGAEALLDRHQEHKGEIDAHEDSFKSADESGQALLAAGHYASDEVREKLTILSEERAALLELWELRRQQYEQCMDLQLFYRDTEQVDNWMSKQEAFLLNEDLGDSLDSVEALLKKHEDFEKSLSAQEEKITALDEFATKLIQNNHYAMEDVATRRDALLSRRNALHERAMYRRAQLADSFHLQQFFRDSDELKSWVNEKMKTATDEAYKDPSNLQGKVQKHQAFEAELSANQSRIDALEKAGQKLIDVDHYAKDEVATRMNEVISLWKKLLEATELKGIKLREANQQQQFNRNVEDIELWLYEVEGHLASDDYGKDLTNVQNLQKKHALLEADVAAHQDRIDGITIQARQFQDAGHFDAENIKKKQEALVARYEALKEPMVARKQKLADSLRLQQLFRDVEDEETWIREKEPIAASTNRGKDLIGVQNLLKKHQALQAEIAGHEPRIKAVTQKGNAMVEEGHFAAEDVKAKLHELNQKWESLKSKASQRRQDLEDSLQAQQYFADANEAESWMREKEPIVGSTDYGKDEDSAEALLKKHEALMSDLSAYGSSIQALREQAQSCRQQVAPMDDETGKELVLALYDYQEKSPREVTMKKGDILTLLNSTNKDWWKVEVNDRQGFVPAAYVKKLDPAQSASRENLLEEQGSIALRQEQIDNQTRVTKEAGSVSLRMKQVEELYHSLLELGEKRKGMLEKSCKKFMLFREANELQQWINEKEAALTSEEVGADLEQVEVLQKKFDDFQKDLKANESRLKDINKVAEDLESEGLMAEEVQAVQQQEVYGAMPRDESDSKTASPWKSARLMVHTVATFNSIKELNERWRSLQQLAEERSQLLGSAHEVQRFHRDADETKEWIEEKNQALNTDNYGHDLASVQALQRKHEGFERDLAALGDKVNSLGETAERLIQSHPESAEDLQEKCTELNQAWSSLGKRADQRKAKLGDSHDLQRFLSDFRDLMSWINGIRGLVSSDELAKDVTGAEALLERHQEHRTEIDARAGTFQAFEQFGQQLLAHGHYASPEIKEKLDVLDQERADLEKAWVQRRMMLDQCLELQLFHRDCEQAENWMAAREAFLNTEDKGDSLDSVEALIKKHEDFDKAINVQEEKIAALQSFADQLIAGGHYAKGDISSRRNEVLDRWRRLKAQMIEKRSKLGESQTLQQFSRDVDEIEAWISEKLQTASDESYKDPTNIQSKHQKHQAFEAELHANADRIRGVIDVGNSLIDRGACAGSEDAVKARLAALADQWQFLVQKSAEKSQKLKEANKQQNFNTGIKDFDFWLSEVEALLASEDYGKDLASVNNLLKKHQLLEADISAHEDRLKDLNSQADSLMTSSAFDTSQVKDKRDTINSRFQKIKSMAASRRARLNESHRLHQFFRDMDDEESWIKEKKLLVSSEDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQFVEHWKELKQLAAARGQRLEESLEYQQFVANVEEEEAWINEKMTLVASEDYGDTLAAIQGLLKKHEAFETDFTVHKDRVNDVCTNGQDLVKKNNHHEENISSKMKGLNGKVSDLEKAASQRKAKLDENSAFLQFNWKADVVESWIGEKENSLKTDDYGRDLSSVQTLLTKQETFDAGLQAFQQEGIANITALKDQLLAAKHVQSKAIEARHAALMKRWSQLLANSATRKKKLLEAQSHFRKVEDLFLTFAKKASAFNSWFENAEEDLTDPVRCNSLEEIKALREAHDAFRSSLSSAQADFNQLAELDRQIKSFRVASNPYTWFTMEALEETWRNLQKIIKERELELQKEQRRQEENDKLRQEFAQHANAFHQWIQETRTYLLDGIAYRRVIRVYQYEVGDDLSGRSCMVEESGTLESQLEATKRKHQEIRAMRSQLKKIEDLGAAMEEALILDNKYTEHSTVGLAQQWDQLDQLGMRMQHNLEQQIQARNTTGVTEEALKEFSMMFKHFDKDKSGRLNHQEFKSCLRSLGYDLPMVEEGEPDPEFEAILDTVDPNRDGHVSLQEYMAFMISRETENVKSSEEIESAFRALSSEGKPYVTKEELYQNLTREQADYCVSHMKPYVDGKGRELPTAFDYVEFTRSLFVN

Target ORF information:

RefSeq Version	XM_012147478.1
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon spectrin, alpha, non-erythrocytic 1 (SPTAN1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012147478.1

ORF Insert Sequence:

ATGCTGTCAT CGTTTCGTAA ACGCAGAGTC CAGAAAATGG ACCCAAGCGG GGTCAAAGTG 
CTGGAGACAG CCGAGGACAT CCAGGAGAGG CGGCAGCAGG TCCTGGACAG ATACCACCGC 
TTCAAGGAGC TCTCGACCCT GAGGCGCCAG AAGCTTGAAG ACTCCTACCG ATTCCAGTTT 
TTCCAAAGAG ATGCTGAGGA GCTGGAGAAA TGGATTCAGG AGAAGCTTCA GATTGCCTCG 
GATGAGAATT ACAAAGACCC AACCAACCTG CAGGGCAAGC TGCAGAAGCA TCAAGCCTTC 
GAAGCCGAGG TGCAGGCCAA CTCGGGTGCC ATCGTGAAGC TTGATGAGAC CGGTAACCTG 
ATGATCTCAG AAGGGCACTT CGCGTCCGAA ACCATCCGGA CTCGTCTGAT GGAGCTGCAC 
CGCCAGTGGG AATTGCTTCT GGAGAAGATG CGGGAGAAAG GCATCAAGCT GCTGCAGGCC 
CAGAAGCTGG TGCAGTACCT GCGGGAGTGT GAGGACGTGA TGGACTGGAT CAATGACAAG 
GAAGCCATTG TGACATCTGA GGAGCTGGGC CAGGACCTGG AGCACGTGGA GGTGTTACAG 
AAGAAATTCG AAGAGTTTCA GACAGACATG GCTGCCCACG AAGAGAGAGT CAATGAAGTG 
AACCAGTTTG CTGCCAAGCT CATTCAGGAG CAGCACCCTG AGGAGGAGCT GATCAAGACA 
AAGGAGGACG AGGTGAATGC AGCCTGGCAG CGGCTGAAGG GCCTGGCCCT CCAGCGGCAG 
GGGAAGCTGT TCGGGGCAGC TGAGGTCCAA CGCTTTAACA GGGATGTGGA CGAGACCATT 
AGCTGGATTA AGGAGAAGGA ACAGTTAATG GCCTCTGATG ACTTTGGCCG GGACCTGGCC 
AGTGTCCAGG CTCTACTGCG GAAACACGAG GGTTTGGAGA GAGATCTTGC TGCCTTGGAG 
GACAAGGTCA AAGCCCTGTG TGCCGAGGCT GACCGCCTGC AGCAGTCCCA CCCTCTGAGT 
GCCACCCAGA TCCAAGTGAA GCGAGAGGAG CTGATCACCA ACTGGGAGCA GATCCGCACC 
CTGGCCGCGG AGAGACACGC ACGTCTCAAC GATTCCTACA GGCTTCAGCG CTTCCTTGCT 
GACTTCCGAG ACCTCACCAG CTGGGTCACT GAGATGAAAG CCCTCATCAA TGCAGATGAA 
CTTGCCAATG ATGTGGCCGG GGCCGAAGCC CTGCTGGATA GGCATCAGGA GCACAAGGGT 
GAAATTGACG CTCATGAAGA TAGCTTTAAA TCTGCAGATG AGTCTGGACA GGCCCTGCTT 
GCAGCTGGTC ACTATGCCTC AGATGAAGTG AGGGAGAAGC TGACCATCCT GTCCGAGGAG 
CGGGCCGCGC TACTAGAGCT CTGGGAGCTG CGACGGCAGC AATATGAGCA GTGCATGGAC 
CTGCAGCTCT TCTACCGCGA CACGGAGCAG GTGGACAACT GGATGAGCAA GCAGGAGGCG 
TTCCTGTTGA ACGAAGACCT GGGTGATTCC TTGGATAGTG TGGAAGCGCT TCTCAAGAAG 
CACGAAGACT TTGAGAAGTC CCTCAGCGCC CAGGAGGAGA AGATCACAGC CTTAGATGAG 
TTTGCCACCA AGCTGATTCA GAACAACCAC TACGCGATGG AAGATGTGGC CACCCGGCGA 
GACGCACTGC TGAGCCGCCG CAACGCCCTC CATGAGAGAG CCATGTACCG CCGTGCCCAG 
CTTGCAGATT CCTTCCACTT GCAGCAGTTT TTCCGTGACT CTGATGAGCT CAAGAGTTGG 
GTCAACGAGA AGATGAAAAC AGCCACAGAT GAAGCTTATA AAGACCCATC CAACCTACAA 
GGGAAAGTGC AGAAGCACCA GGCCTTTGAG GCCGAGCTCT CAGCAAACCA GAGCCGCATC 
GACGCCTTGG AGAAAGCTGG GCAGAAGCTG ATCGACGTTG ACCACTATGC CAAGGATGAG 
GTGGCGACTC GGATGAATGA AGTTATCAGT CTGTGGAAGA AGCTGCTGGA GGCCACGGAG 
CTGAAAGGCA TAAAGCTCCG TGAGGCTAAC CAGCAGCAGC AGTTTAACCG CAATGTTGAG 
GACATCGAGC TGTGGCTGTA CGAAGTGGAA GGTCATCTGG CCTCGGATGA CTACGGCAAA 
GACCTCACCA ACGTCCAGAA CCTCCAGAAA AAGCATGCCC TGCTGGAGGC TGACGTGGCC 
GCTCACCAGG ATCGCATCGA TGGCATCACT ATCCAAGCCC GCCAGTTCCA GGATGCGGGC 
CACTTTGATG CTGAAAACAT CAAGAAGAAG CAGGAGGCCC TGGTAGCTCG CTACGAGGCA 
CTCAAGGAGC CCATGGTTGC CCGGAAGCAG AAGCTGGCCG ATTCCCTGCG GTTGCAGCAG 
CTCTTCCGTG ACGTTGAGGA TGAGGAGACG TGGATTCGGG AGAAAGAACC TATTGCCGCC 
TCCACCAACA GAGGCAAAGA TTTAATTGGA GTCCAGAACC TGCTAAAGAA ACACCAAGCG 
CTACAAGCAG AAATTGCTGG CCACGAGCCC CGCATCAAAG CAGTCACACA GAAGGGGAAC 
GCCATGGTAG AGGAGGGCCA TTTTGCTGCC GAGGACGTGA AGGCCAAGCT CCATGAACTG 
AACCAGAAGT GGGAGTCGCT GAAGTCCAAG GCCTCCCAGC GACGGCAGGA CCTGGAGGAC 
TCGCTGCAGG CCCAGCAGTA CTTCGCTGAT GCCAACGAGG CCGAGTCCTG GATGCGGGAG 
AAGGAGCCCA TCGTGGGCAG CACCGACTAT GGCAAAGATG AGGACTCTGC CGAGGCTCTG 
CTAAAGAAGC ACGAGGCATT GATGTCGGAT CTCAGCGCCT ATGGCAGCAG CATCCAGGCC 
CTGCGAGAGC AGGCCCAGTC GTGCCGGCAA CAAGTGGCCC CCATGGATGA CGAGACTGGG 
AAGGAGCTGG TCTTGGCGCT CTATGACTAT CAGGAGAAGA GTCCCCGAGA GGTCACCATG 
AAGAAAGGAG ACATTCTCAC CTTACTCAAC AGCACCAACA AGGACTGGTG GAAAGTGGAA 
GTGAACGACC GGCAGGGCTT CGTGCCGGCC GCCTACGTGA AGAAGCTGGA CCCAGCGCAG 
TCGGCCTCCC GGGAGAACCT CCTGGAAGAG CAGGGCAGCA TCGCGCTGCG GCAGGAGCAG 
ATTGACAACC AGACGCGCGT AACTAAGGAG GCCGGCAGCG TGTCTCTGCG TATGAAACAG 
GTGGAAGAAC TGTACCATTC TCTGCTGGAA CTGGGAGAGA AGCGTAAAGG CATGTTGGAG 
AAGAGCTGTA AGAAGTTCAT GCTGTTCCGT GAAGCGAACG AGCTGCAGCA GTGGATCAAC 
GAGAAGGAAG CGGCCCTGAC AAGCGAGGAG GTCGGCGCGG ACTTGGAGCA GGTCGAAGTG 
CTGCAGAAGA AGTTTGACGA CTTCCAGAAG GATCTCAAGG CCAATGAGTC ACGGCTGAAG 
GACATTAACA AGGTGGCTGA AGACCTGGAG TCGGAAGGCC TGATGGCGGA GGAGGTGCAG 
GCCGTGCAGC AGCAGGAGGT GTATGGCGCG ATGCCCAGGG ATGAATCTGA TTCCAAGACA 
GCCTCCCCGT GGAAGTCTGC TCGCCTGATG GTTCACACCG TGGCCACCTT TAATTCCATC 
AAGGAGCTCA ATGAGCGCTG GCGGTCCCTG CAGCAGCTGG CTGAGGAGCG GAGCCAACTC 
TTGGGCAGCG CCCACGAAGT GCAGCGGTTC CACAGAGATG CTGACGAAAC CAAAGAGTGG 
ATCGAGGAGA AGAACCAGGC TCTAAACACA GACAACTATG GCCACGACCT GGCCAGCGTC 
CAGGCCCTGC AGCGCAAGCA TGAGGGCTTC GAGAGGGACC TCGCGGCCCT TGGCGACAAG 
GTGAACTCGC TCGGGGAGAC AGCCGAGCGC CTGATCCAGT CCCACCCCGA GTCGGCAGAG 
GACCTGCAGG AGAAGTGCAC TGAGCTGAAC CAGGCATGGA GCAGCCTGGG CAAGCGTGCC 
GACCAGCGCA AGGCCAAGCT GGGCGACTCC CACGACCTGC AGCGCTTCCT CAGCGACTTC 
CGGGACCTCA TGTCTTGGAT CAATGGAATC CGAGGTCTGG TGTCCTCAGA CGAGCTAGCC 
AAGGATGTCA CTGGAGCTGA GGCCCTGCTG GAGCGACACC AGGAACACCG GACAGAAATT 
GATGCCAGAG CTGGCACTTT CCAGGCATTC GAGCAGTTTG GGCAGCAGCT GTTGGCTCAT 
GGGCACTACG CCAGCCCCGA GATCAAGGAG AAGCTCGATG TTCTAGACCA GGAGCGCGCA 
GACCTCGAGA AGGCCTGGGT CCAGCGCAGG ATGATGCTGG ATCAGTGCCT TGAATTGCAG 
CTGTTCCATC GGGATTGCGA GCAGGCCGAG AACTGGATGG CTGCCCGGGA GGCCTTCCTG 
AACACAGAAG ACAAAGGGGA CTCGCTAGAC AGCGTGGAGG CTCTGATCAA AAAACACGAG 
GACTTTGACA AAGCAATTAA TGTCCAGGAG GAGAAGATTG CTGCCCTGCA GTCCTTTGCT 
GACCAGCTCA TTGCTGGCGG TCACTACGCC AAAGGCGACA TCTCCAGCCG GCGCAATGAG 
GTTCTGGACA GGTGGCGCCG TCTAAAAGCT CAGATGATTG AGAAGAGGTC AAAGCTGGGA 
GAATCGCAGA CCCTCCAGCA GTTCAGCCGG GATGTAGACG AGATTGAAGC TTGGATCAGT 
GAAAAATTAC AGACTGCAAG TGATGAGTCA TATAAGGACC CCACCAACAT CCAGAGCAAG 
CATCAGAAGC ACCAGGCCTT CGAGGCAGAA CTGCACGCCA ATGCGGACCG GATCCGCGGG 
GTCATCGACG TGGGCAACTC CCTCATTGAC CGTGGGGCCT GTGCCGGCAG CGAGGATGCC 
GTCAAGGCCC GGCTGGCCGC CTTAGCTGAC CAGTGGCAGT TCCTGGTGCA GAAGTCGGCT 
GAGAAGAGCC AGAAGCTGAA AGAAGCCAAC AAACAGCAGA ACTTCAACAC TGGCATCAAG 
GACTTCGACT TCTGGCTCTC TGAGGTGGAA GCCCTGCTGG CCTCTGAGGA TTATGGCAAA 
GACCTGGCTT CTGTGAACAA CTTGCTAAAA AAACATCAGC TGCTGGAGGC AGATATCTCT 
GCCCACGAGG ACCGCCTGAA GGACCTCAAC AGCCAGGCGG ACAGCCTGAT GACCAGCAGC 
GCCTTCGACA CCTCCCAGGT GAAGGATAAG CGGGACACCA TCAACAGCCG TTTCCAGAAG 
ATCAAGAGCA TGGCCGCCTC CCGGCGAGCT AGGCTGAATG AGTCCCACCG CCTGCACCAG 
TTCTTCCGGG ACATGGACGA TGAGGAGTCC TGGATCAAGG AGAAGAAGCT GCTGGTGAGC 
TCAGAGGACT ACGGCCGCGA CCTGACGGGC GTCCAGAACC TGAGGAAGAA GCACAAACGG 
CTGGAGGCAG AGCTGGCTGC CCACGAGCCG GCCATTCAGG GTGTCCTGGA CACTGGAAAG 
AAGCTGTCTG ATGACAACAC CATCGGGAAG GAGGAGATTC AGCAGCGCCT GGCGCAGTTT 
GTGGAGCACT GGAAGGAGCT GAAGCAGCTG GCAGCTGCTC GGGGCCAGCG GCTAGAAGAA 
TCTTTGGAAT ATCAGCAGTT TGTGGCCAAC GTGGAGGAGG AGGAAGCCTG GATCAATGAG 
AAGATGACCC TGGTGGCCAG TGAGGATTAC GGAGACACTC TTGCTGCCAT CCAGGGCTTG 
CTGAAGAAAC ACGAAGCCTT CGAGACGGAC TTCACTGTCC ACAAGGACCG GGTGAATGAC 
GTCTGCACTA ACGGACAAGA CCTGGTTAAG AAGAACAATC ACCATGAGGA GAACATCTCT 
TCCAAGATGA AGGGCTTGAA CGGGAAAGTG TCAGACTTGG AGAAGGCTGC CTCCCAGAGG 
AAGGCAAAGC TCGACGAGAA CTCGGCCTTC CTCCAGTTCA ACTGGAAGGC CGATGTGGTG 
GAGTCCTGGA TTGGTGAAAA GGAGAACAGC TTGAAGACAG ATGATTATGG TCGAGATCTG 
TCTTCTGTCC AAACTCTCCT CACCAAACAG GAAACCTTCG ATGCTGGCCT GCAGGCCTTC 
CAGCAGGAGG GCATCGCCAA CATCACTGCC CTCAAAGATC AGCTGCTGGC CGCCAAGCAT 
GTTCAGTCCA AGGCCATCGA GGCCCGCCAC GCTGCCCTCA TGAAGAGATG GAGCCAGCTC 
CTGGCCAATT CGGCCACCCG GAAGAAGAAG CTGCTGGAGG CACAGAGTCA CTTCCGCAAG 
GTGGAGGATC TCTTCCTGAC CTTTGCCAAA AAGGCTTCAG CCTTCAACAG CTGGTTTGAA 
AATGCAGAGG AGGACTTAAC AGACCCAGTG CGCTGCAACT CCCTGGAAGA GATCAAGGCC 
TTGAGAGAGG CCCACGATGC CTTCCGCTCC TCGCTGAGCT CAGCCCAGGC CGACTTCAAC 
CAGCTGGCTG AGCTGGACCG CCAGATCAAG AGCTTCCGCG TGGCCTCCAA CCCCTACACC 
TGGTTCACCA TGGAGGCCCT GGAGGAGACC TGGAGGAACC TTCAGAAGAT CATCAAGGAA 
AGGGAGCTGG AGCTGCAGAA GGAGCAGCGG CGGCAGGAGG AGAACGACAA GCTGCGCCAG 
GAGTTCGCCC AGCATGCCAA CGCCTTCCAC CAGTGGATCC AGGAGACCAG AACGTATCTC 
CTCGATGGCA TAGCGTATCG TCGGGTCATT CGTGTCTATC AGTATGAAGT TGGGGATGAT 
CTGTCTGGAA GGTCCTGCAT GGTGGAAGAA TCTGGGACCC TTGAATCGCA GCTTGAAGCT 
ACCAAACGCA AACACCAGGA GATTCGAGCC ATGAGAAGTC AGCTCAAGAA GATTGAAGAC 
CTGGGGGCGG CCATGGAGGA AGCGCTGATC CTGGACAACA AGTACACGGA GCACAGCACC 
GTGGGCCTGG CCCAGCAGTG GGACCAGCTC GACCAGCTGG GCATGCGGAT GCAGCACAAC 
CTGGAGCAGC AGATCCAGGC CAGGAACACG ACAGGAGTGA CCGAGGAGGC CCTCAAAGAA 
TTCAGCATGA TGTTCAAACA CTTCGACAAG GACAAGTCCG GCCGGCTGAA TCACCAGGAG 
TTCAAATCCT GTCTGCGCTC CCTGGGCTAT GACCTGCCCA TGGTGGAGGA AGGGGAGCCC 
GACCCTGAGT TCGAGGCCAT CTTGGATACT GTGGATCCTA ACAGGGACGG CCACGTCTCC 
CTGCAAGAGT ACATGGCCTT TATGATCAGC CGTGAGACCG AGAATGTCAA GTCCAGCGAG 
GAGATTGAGA GCGCCTTCCG GGCCCTGAGC TCCGAAGGGA AGCCTTATGT GACCAAGGAG 
GAGCTGTACC AGAACCTGAC TCGGGAACAA GCCGACTACT GCGTCTCCCA CATGAAGCCC 
TACGTGGACG GCAAGGGCCG CGAACTCCCC ACTGCTTTCG ACTACGTTGA GTTCACCCGC 
TCGCTCTTCG TGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTAN1 ( XM_012147479.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_012002869.1 Ovis aries musimon spectrin, alpha, non-erythrocytic 1 (SPTAN1), transcript variant X4, mRNA.

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CloneID	OOv24451
Clone ID Related Accession (Same CDS sequence)	XM_012147479.2 , XM_012147480.2
Accession Version	XM_012147479.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7497bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	spectrin alpha chain, non-erythrocytic 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942583.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012147479.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCCAA GCGGGGTCAA AGTGCTGGAG ACAGCCGAGG ACATCCAGGA GAGGCGGCAG 
CAGGTCCTGG ACAGATACCA CCGCTTCAAG GAGCTCTCGA CCCTGAGGCG CCAGAAGCTT 
GAAGACTCCT ACCGATTCCA GTTTTTCCAA AGAGATGCTG AGGAGCTGGA GAAATGGATT 
CAGGAGAAGC TTCAGATTGC CTCGGATGAG AATTACAAAG ACCCAACCAA CCTGCAGGGC 
AAGCTGCAGA AGCATCAAGC CTTCGAAGCC GAGGTGCAGG CCAACTCGGG TGCCATCGTG 
AAGCTTGATG AGACCGGTAA CCTGATGATC TCAGAAGGGC ACTTCGCGTC CGAAACCATC 
CGGACTCGTC TGATGGAGCT GCACCGCCAG TGGGAATTGC TTCTGGAGAA GATGCGGGAG 
AAAGGCATCA AGCTGCTGCA GGCCCAGAAG CTGGTGCAGT ACCTGCGGGA GTGTGAGGAC 
GTGATGGACT GGATCAATGA CAAGGAAGCC ATTGTGACAT CTGAGGAGCT GGGCCAGGAC 
CTGGAGCACG TGGAGGTGTT ACAGAAGAAA TTCGAAGAGT TTCAGACAGA CATGGCTGCC 
CACGAAGAGA GAGTCAATGA AGTGAACCAG TTTGCTGCCA AGCTCATTCA GGAGCAGCAC 
CCTGAGGAGG AGCTGATCAA GACAAAGGAG GACGAGGTGA ATGCAGCCTG GCAGCGGCTG 
AAGGGCCTGG CCCTCCAGCG GCAGGGGAAG CTGTTCGGGG CAGCTGAGGT CCAACGCTTT 
AACAGGGATG TGGACGAGAC CATTAGCTGG ATTAAGGAGA AGGAACAGTT AATGGCCTCT 
GATGACTTTG GCCGGGACCT GGCCAGTGTC CAGGCTCTAC TGCGGAAACA CGAGGGTTTG 
GAGAGAGATC TTGCTGCCTT GGAGGACAAG GTCAAAGCCC TGTGTGCCGA GGCTGACCGC 
CTGCAGCAGT CCCACCCTCT GAGTGCCACC CAGATCCAAG TGAAGCGAGA GGAGCTGATC 
ACCAACTGGG AGCAGATCCG CACCCTGGCC GCGGAGAGAC ACGCACGTCT CAACGATTCC 
TACAGGCTTC AGCGCTTCCT TGCTGACTTC CGAGACCTCA CCAGCTGGGT CACTGAGATG 
AAAGCCCTCA TCAATGCAGA TGAACTTGCC AATGATGTGG CCGGGGCCGA AGCCCTGCTG 
GATAGGCATC AGGAGCACAA GGGTGAAATT GACGCTCATG AAGATAGCTT TAAATCTGCA 
GATGAGTCTG GACAGGCCCT GCTTGCAGCT GGTCACTATG CCTCAGATGA AGTGAGGGAG 
AAGCTGACCA TCCTGTCCGA GGAGCGGGCC GCGCTACTAG AGCTCTGGGA GCTGCGACGG 
CAGCAATATG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GCGACACGGA GCAGGTGGAC 
AACTGGATGA GCAAGCAGGA GGCGTTCCTG TTGAACGAAG ACCTGGGTGA TTCCTTGGAT 
AGTGTGGAAG CGCTTCTCAA GAAGCACGAA GACTTTGAGA AGTCCCTCAG CGCCCAGGAG 
GAGAAGATCA CAGCCTTAGA TGAGTTTGCC ACCAAGCTGA TTCAGAACAA CCACTACGCG 
ATGGAAGATG TGGCCACCCG GCGAGACGCA CTGCTGAGCC GCCGCAACGC CCTCCATGAG 
AGAGCCATGT ACCGCCGTGC CCAGCTTGCA GATTCCTTCC ACTTGCAGCA GTTTTTCCGT 
GACTCTGATG AGCTCAAGAG TTGGGTCAAC GAGAAGATGA AAACAGCCAC AGATGAAGCT 
TATAAAGACC CATCCAACCT ACAAGGGAAA GTGCAGAAGC ACCAGGCCTT TGAGGCCGAG 
CTCTCAGCAA ACCAGAGCCG CATCGACGCC TTGGAGAAAG CTGGGCAGAA GCTGATCGAC 
GTTGACCACT ATGCCAAGGA TGAGGTGGCG ACTCGGATGA ATGAAGTTAT CAGTCTGTGG 
AAGAAGCTGC TGGAGGCCAC GGAGCTGAAA GGCATAAAGC TCCGTGAGGC TAACCAGCAG 
CAGCAGTTTA ACCGCAATGT TGAGGACATC GAGCTGTGGC TGTACGAAGT GGAAGGTCAT 
CTGGCCTCGG ATGACTACGG CAAAGACCTC ACCAACGTCC AGAACCTCCA GAAAAAGCAT 
GCCCTGCTGG AGGCTGACGT GGCCGCTCAC CAGGATCGCA TCGATGGCAT CACTATCCAA 
GCCCGCCAGT TCCAGGATGC GGGCCACTTT GATGCTGAAA ACATCAAGAA GAAGCAGGAG 
GCCCTGGTAG CTCGCTACGA GGCACTCAAG GAGCCCATGG TTGCCCGGAA GCAGAAGCTG 
GCCGATTCCC TGCGGTTGCA GCAGCTCTTC CGTGACGTTG AGGATGAGGA GACGTGGATT 
CGGGAGAAAG AACCTATTGC CGCCTCCACC AACAGAGGCA AAGATTTAAT TGGAGTCCAG 
AACCTGCTAA AGAAACACCA AGCGCTACAA GCAGAAATTG CTGGCCACGA GCCCCGCATC 
AAAGCAGTCA CACAGAAGGG GAACGCCATG GTAGAGGAGG GCCATTTTGC TGCCGAGGAC 
GTGAAGGCCA AGCTCCATGA ACTGAACCAG AAGTGGGAGT CGCTGAAGTC CAAGGCCTCC 
CAGCGACGGC AGGACCTGGA GGACTCGCTG CAGGCCCAGC AGTACTTCGC TGATGCCAAC 
GAGGCCGAGT CCTGGATGCG GGAGAAGGAG CCCATCGTGG GCAGCACCGA CTATGGCAAA 
GATGAGGACT CTGCCGAGGC TCTGCTAAAG AAGCACGAGG CATTGATGTC GGATCTCAGC 
GCCTATGGCA GCAGCATCCA GGCCCTGCGA GAGCAGGCCC AGTCGTGCCG GCAACAAGTG 
GCCCCCATGG ATGACGAGAC TGGGAAGGAG CTGGTCTTGG CGCTCTATGA CTATCAGGAG 
AAGAGTCCCC GAGAGGTCAC CATGAAGAAA GGAGACATTC TCACCTTACT CAACAGCACC 
AACAAGGACT GGTGGAAAGT GGAAGTGAAC GACCGGCAGG GCTTCGTGCC GGCCGCCTAC 
GTGAAGAAGC TGGACCCAGC GCAGTCGGCC TCCCGGGAGA ACCTCCTGGA AGAGCAGGGC 
AGCATCGCGC TGCGGCAGGA GCAGATTGAC AACCAGACGC GCGTAACTAA GGAGGCCGGC 
AGCGTGTCTC TGCGTATGAA ACAGGTGGAA GAACTGTACC ATTCTCTGCT GGAACTGGGA 
GAGAAGCGTA AAGGCATGTT GGAGAAGAGC TGTAAGAAGT TCATGCTGTT CCGTGAAGCG 
AACGAGCTGC AGCAGTGGAT CAACGAGAAG GAAGCGGCCC TGACAAGCGA GGAGGTCGGC 
GCGGACTTGG AGCAGGTCGA AGTGCTGCAG AAGAAGTTTG ACGACTTCCA GAAGGATCTC 
AAGGCCAATG AGTCACGGCT GAAGGACATT AACAAGGTGG CTGAAGACCT GGAGTCGGAA 
GGCCTGATGG CGGAGGAGGT GCAGGCCGTG CAGCAGCAGG AGGTGTATGG CGCGATGCCC 
AGGGATGAAT CTGATTCCAA GACAGCCTCC CCGTGGAAGT CTGCTCGCCT GATGGTTCAC 
ACCGTGGCCA CCTTTAATTC CATCAAGGAG CTCAATGAGC GCTGGCGGTC CCTGCAGCAG 
CTGGCTGAGG AGCGGAGCCA ACTCTTGGGC AGCGCCCACG AAGTGCAGCG GTTCCACAGA 
GATGCTGACG AAACCAAAGA GTGGATCGAG GAGAAGAACC AGGCTCTAAA CACAGACAAC 
TATGGCCACG ACCTGGCCAG CGTCCAGGCC CTGCAGCGCA AGCATGAGGG CTTCGAGAGG 
GACCTCGCGG CCCTTGGCGA CAAGGTGAAC TCGCTCGGGG AGACAGCCGA GCGCCTGATC 
CAGTCCCACC CCGAGTCGGC AGAGGACCTG CAGGAGAAGT GCACTGAGCT GAACCAGGCA 
TGGAGCAGCC TGGGCAAGCG TGCCGACCAG CGCAAGGCCA AGCTGGGCGA CTCCCACGAC 
CTGCAGCGCT TCCTCAGCGA CTTCCGGGAC CTCATGTCTT GGATCAATGG AATCCGAGGT 
CTGGTGTCCT CAGACGAGCT AGCCAAGGAT GTCACTGGAG CTGAGGCCCT GCTGGAGCGA 
CACCAGGAAC ACCGGACAGA AATTGATGCC AGAGCTGGCA CTTTCCAGGC ATTCGAGCAG 
TTTGGGCAGC AGCTGTTGGC TCATGGGCAC TACGCCAGCC CCGAGATCAA GGAGAAGCTC 
GATGTTCTAG ACCAGGAGCG CGCAGACCTC GAGAAGGCCT GGGTCCAGCG CAGGATGATG 
CTGGATCAGT GCCTTGAATT GCAGCTGTTC CATCGGGATT GCGAGCAGGC CGAGAACTGG 
ATGGCTGCCC GGGAGGCCTT CCTGAACACA GAAGACAAAG GGGACTCGCT AGACAGCGTG 
GAGGCTCTGA TCAAAAAACA CGAGGACTTT GACAAAGCAA TTAATGTCCA GGAGGAGAAG 
ATTGCTGCCC TGCAGTCCTT TGCTGACCAG CTCATTGCTG GCGGTCACTA CGCCAAAGGC 
GACATCTCCA GCCGGCGCAA TGAGGTTCTG GACAGGTGGC GCCGTCTAAA AGCTCAGATG 
ATTGAGAAGA GGTCAAAGCT GGGAGAATCG CAGACCCTCC AGCAGTTCAG CCGGGATGTA 
GACGAGATTG AAGCTTGGAT CAGTGAAAAA TTACAGACTG CAAGTGATGA GTCATATAAG 
GACCCCACCA ACATCCAGCT TTCCAAGCTG CTGAGCAAGC ATCAGAAGCA CCAGGCCTTC 
GAGGCAGAAC TGCACGCCAA TGCGGACCGG ATCCGCGGGG TCATCGACGT GGGCAACTCC 
CTCATTGACC GTGGGGCCTG TGCCGGCAGC GAGGATGCCG TCAAGGCCCG GCTGGCCGCC 
TTAGCTGACC AGTGGCAGTT CCTGGTGCAG AAGTCGGCTG AGAAGAGCCA GAAGCTGAAA 
GAAGCCAACA AACAGCAGAA CTTCAACACT GGCATCAAGG ACTTCGACTT CTGGCTCTCT 
GAGGTGGAAG CCCTGCTGGC CTCTGAGGAT TATGGCAAAG ACCTGGCTTC TGTGAACAAC 
TTGCTAAAAA AACATCAGCT GCTGGAGGCA GATATCTCTG CCCACGAGGA CCGCCTGAAG 
GACCTCAACA GCCAGGCGGA CAGCCTGATG ACCAGCAGCG CCTTCGACAC CTCCCAGGTG 
AAGGATAAGC GGGACACCAT CAACAGCCGT TTCCAGAAGA TCAAGAGCAT GGCCGCCTCC 
CGGCGAGCTA GGCTGAATGA GTCCCACCGC CTGCACCAGT TCTTCCGGGA CATGGACGAT 
GAGGAGTCCT GGATCAAGGA GAAGAAGCTG CTGGTGAGCT CAGAGGACTA CGGCCGCGAC 
CTGACGGGCG TCCAGAACCT GAGGAAGAAG CACAAACGGC TGGAGGCAGA GCTGGCTGCC 
CACGAGCCGG CCATTCAGGG TGTCCTGGAC ACTGGAAAGA AGCTGTCTGA TGACAACACC 
ATCGGGAAGG AGGAGATTCA GCAGCGCCTG GCGCAGTTTG TGGAGCACTG GAAGGAGCTG 
AAGCAGCTGG CAGCTGCTCG GGGCCAGCGG CTAGAAGAAT CTTTGGAATA TCAGCAGTTT 
GTGGCCAACG TGGAGGAGGA GGAAGCCTGG ATCAATGAGA AGATGACCCT GGTGGCCAGT 
GAGGATTACG GAGACACTCT TGCTGCCATC CAGGGCTTGC TGAAGAAACA CGAAGCCTTC 
GAGACGGACT TCACTGTCCA CAAGGACCGG GTGAATGACG TCTGCACTAA CGGACAAGAC 
CTGGTTAAGA AGAACAATCA CCATGAGGAG AACATCTCTT CCAAGATGAA GGGCTTGAAC 
GGGAAAGTGT CAGACTTGGA GAAGGCTGCC TCCCAGAGGA AGGCAAAGCT CGACGAGAAC 
TCGGCCTTCC TCCAGTTCAA CTGGAAGGCC GATGTGGTGG AGTCCTGGAT TGGTGAAAAG 
GAGAACAGCT TGAAGACAGA TGATTATGGT CGAGATCTGT CTTCTGTCCA AACTCTCCTC 
ACCAAACAGG AAACCTTCGA TGCTGGCCTG CAGGCCTTCC AGCAGGAGGG CATCGCCAAC 
ATCACTGCCC TCAAAGATCA GCTGCTGGCC GCCAAGCATG TTCAGTCCAA GGCCATCGAG 
GCCCGCCACG CTGCCCTCAT GAAGAGATGG AGCCAGCTCC TGGCCAATTC GGCCACCCGG 
AAGAAGAAGC TGCTGGAGGC ACAGAGTCAC TTCCGCAAGG TGGAGGATCT CTTCCTGACC 
TTTGCCAAAA AGGCTTCAGC CTTCAACAGC TGGTTTGAAA ATGCAGAGGA GGACTTAACA 
GACCCAGTGC GCTGCAACTC CCTGGAAGAG ATCAAGGCCT TGAGAGAGGC CCACGATGCC 
TTCCGCTCCT CGCTGAGCTC AGCCCAGGCC GACTTCAACC AGCTGGCTGA GCTGGACCGC 
CAGATCAAGA GCTTCCGCGT GGCCTCCAAC CCCTACACCT GGTTCACCAT GGAGGCCCTG 
GAGGAGACCT GGAGGAACCT TCAGAAGATC ATCAAGGAAA GGGAGCTGGA GCTGCAGAAG 
GAGCAGCGGC GGCAGGAGGA GAACGACAAG CTGCGCCAGG AGTTCGCCCA GCATGCCAAC 
GCCTTCCACC AGTGGATCCA GGAGACCAGA ACGTATCTCC TCGATGGCAT AGCGTATCGT 
CGGGTCATTC GTGTCTATCA GTATGAAGTT GGGGATGATC TGTCTGGAAG GTCCTGCATG 
GTGGAAGAAT CTGGGACCCT TGAATCGCAG CTTGAAGCTA CCAAACGCAA ACACCAGGAG 
ATTCGAGCCA TGAGAAGTCA GCTCAAGAAG ATTGAAGACC TGGGGGCGGC CATGGAGGAA 
GCGCTGATCC TGGACAACAA GTACACGGAG CACAGCACCG TGGGCCTGGC CCAGCAGTGG 
GACCAGCTCG ACCAGCTGGG CATGCGGATG CAGCACAACC TGGAGCAGCA GATCCAGGCC 
AGGAACACGA CAGGAGTGAC CGAGGAGGCC CTCAAAGAAT TCAGCATGAT GTTCAAACAC 
TTCGACAAGG ACAAGTCCGG CCGGCTGAAT CACCAGGAGT TCAAATCCTG TCTGCGCTCC 
CTGGGCTATG ACCTGCCCAT GGTGGAGGAA GGGGAGCCCG ACCCTGAGTT CGAGGCCATC 
TTGGATACTG TGGATCCTAA CAGGGACGGC CACGTCTCCC TGCAAGAGTA CATGGCCTTT 
ATGATCAGCC GTGAGACCGA GAATGTCAAG TCCAGCGAGG AGATTGAGAG CGCCTTCCGG 
GCCCTGAGCT CCGAAGGGAA GCCTTATGTG ACCAAGGAGG AGCTGTACCA GAACCTGACT 
CGGGAACAAG CCGACTACTG CGTCTCCCAC ATGAAGCCCT ACGTGGACGG CAAGGGCCGC 
GAACTCCCCA CTGCTTTCGA CTACGTTGAG TTCACCCGCT CGCTCTTCGT GAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012002869.1
CDS	159..7655
Translation	MDPSGVKVLETAEDIQERRQQVLDRYHRFKELSTLRRQKLEDSYRFQFFQRDAEELEKWIQEKLQIASDENYKDPTNLQGKLQKHQAFEAEVQANSGAIVKLDETGNLMISEGHFASETIRTRLMELHRQWELLLEKMREKGIKLLQAQKLVQYLRECEDVMDWINDKEAIVTSEELGQDLEHVEVLQKKFEEFQTDMAAHEERVNEVNQFAAKLIQEQHPEEELIKTKEDEVNAAWQRLKGLALQRQGKLFGAAEVQRFNRDVDETISWIKEKEQLMASDDFGRDLASVQALLRKHEGLERDLAALEDKVKALCAEADRLQQSHPLSATQIQVKREELITNWEQIRTLAAERHARLNDSYRLQRFLADFRDLTSWVTEMKALINADELANDVAGAEALLDRHQEHKGEIDAHEDSFKSADESGQALLAAGHYASDEVREKLTILSEERAALLELWELRRQQYEQCMDLQLFYRDTEQVDNWMSKQEAFLLNEDLGDSLDSVEALLKKHEDFEKSLSAQEEKITALDEFATKLIQNNHYAMEDVATRRDALLSRRNALHERAMYRRAQLADSFHLQQFFRDSDELKSWVNEKMKTATDEAYKDPSNLQGKVQKHQAFEAELSANQSRIDALEKAGQKLIDVDHYAKDEVATRMNEVISLWKKLLEATELKGIKLREANQQQQFNRNVEDIELWLYEVEGHLASDDYGKDLTNVQNLQKKHALLEADVAAHQDRIDGITIQARQFQDAGHFDAENIKKKQEALVARYEALKEPMVARKQKLADSLRLQQLFRDVEDEETWIREKEPIAASTNRGKDLIGVQNLLKKHQALQAEIAGHEPRIKAVTQKGNAMVEEGHFAAEDVKAKLHELNQKWESLKSKASQRRQDLEDSLQAQQYFADANEAESWMREKEPIVGSTDYGKDEDSAEALLKKHEALMSDLSAYGSSIQALREQAQSCRQQVAPMDDETGKELVLALYDYQEKSPREVTMKKGDILTLLNSTNKDWWKVEVNDRQGFVPAAYVKKLDPAQSASRENLLEEQGSIALRQEQIDNQTRVTKEAGSVSLRMKQVEELYHSLLELGEKRKGMLEKSCKKFMLFREANELQQWINEKEAALTSEEVGADLEQVEVLQKKFDDFQKDLKANESRLKDINKVAEDLESEGLMAEEVQAVQQQEVYGAMPRDESDSKTASPWKSARLMVHTVATFNSIKELNERWRSLQQLAEERSQLLGSAHEVQRFHRDADETKEWIEEKNQALNTDNYGHDLASVQALQRKHEGFERDLAALGDKVNSLGETAERLIQSHPESAEDLQEKCTELNQAWSSLGKRADQRKAKLGDSHDLQRFLSDFRDLMSWINGIRGLVSSDELAKDVTGAEALLERHQEHRTEIDARAGTFQAFEQFGQQLLAHGHYASPEIKEKLDVLDQERADLEKAWVQRRMMLDQCLELQLFHRDCEQAENWMAAREAFLNTEDKGDSLDSVEALIKKHEDFDKAINVQEEKIAALQSFADQLIAGGHYAKGDISSRRNEVLDRWRRLKAQMIEKRSKLGESQTLQQFSRDVDEIEAWISEKLQTASDESYKDPTNIQLSKLLSKHQKHQAFEAELHANADRIRGVIDVGNSLIDRGACAGSEDAVKARLAALADQWQFLVQKSAEKSQKLKEANKQQNFNTGIKDFDFWLSEVEALLASEDYGKDLASVNNLLKKHQLLEADISAHEDRLKDLNSQADSLMTSSAFDTSQVKDKRDTINSRFQKIKSMAASRRARLNESHRLHQFFRDMDDEESWIKEKKLLVSSEDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQFVEHWKELKQLAAARGQRLEESLEYQQFVANVEEEEAWINEKMTLVASEDYGDTLAAIQGLLKKHEAFETDFTVHKDRVNDVCTNGQDLVKKNNHHEENISSKMKGLNGKVSDLEKAASQRKAKLDENSAFLQFNWKADVVESWIGEKENSLKTDDYGRDLSSVQTLLTKQETFDAGLQAFQQEGIANITALKDQLLAAKHVQSKAIEARHAALMKRWSQLLANSATRKKKLLEAQSHFRKVEDLFLTFAKKASAFNSWFENAEEDLTDPVRCNSLEEIKALREAHDAFRSSLSSAQADFNQLAELDRQIKSFRVASNPYTWFTMEALEETWRNLQKIIKERELELQKEQRRQEENDKLRQEFAQHANAFHQWIQETRTYLLDGIAYRRVIRVYQYEVGDDLSGRSCMVEESGTLESQLEATKRKHQEIRAMRSQLKKIEDLGAAMEEALILDNKYTEHSTVGLAQQWDQLDQLGMRMQHNLEQQIQARNTTGVTEEALKEFSMMFKHFDKDKSGRLNHQEFKSCLRSLGYDLPMVEEGEPDPEFEAILDTVDPNRDGHVSLQEYMAFMISRETENVKSSEEIESAFRALSSEGKPYVTKEELYQNLTREQADYCVSHMKPYVDGKGRELPTAFDYVEFTRSLFVN

Target ORF information:

RefSeq Version	XM_012147479.2
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon spectrin, alpha, non-erythrocytic 1 (SPTAN1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012147479.2

ORF Insert Sequence:

ATGGACCCAA GCGGGGTCAA AGTGCTGGAG ACAGCCGAGG ACATCCAGGA GAGGCGGCAG 
CAGGTCCTGG ACAGATACCA CCGCTTCAAG GAGCTCTCGA CCCTGAGGCG CCAGAAGCTT 
GAAGACTCCT ACCGATTCCA GTTTTTCCAA AGAGATGCTG AGGAGCTGGA GAAATGGATT 
CAGGAGAAGC TTCAGATTGC CTCGGATGAG AATTACAAAG ACCCAACCAA CCTGCAGGGC 
AAGCTGCAGA AGCATCAAGC CTTCGAAGCC GAGGTGCAGG CCAACTCGGG TGCCATCGTG 
AAGCTTGATG AGACCGGTAA CCTGATGATC TCAGAAGGGC ACTTCGCGTC CGAAACCATC 
CGGACTCGTC TGATGGAGCT GCACCGCCAG TGGGAATTGC TTCTGGAGAA GATGCGGGAG 
AAAGGCATCA AGCTGCTGCA GGCCCAGAAG CTGGTGCAGT ACCTGCGGGA GTGTGAGGAC 
GTGATGGACT GGATCAATGA CAAGGAAGCC ATTGTGACAT CTGAGGAGCT GGGCCAGGAC 
CTGGAGCACG TGGAGGTGTT ACAGAAGAAA TTCGAAGAGT TTCAGACAGA CATGGCTGCC 
CACGAAGAGA GAGTCAATGA AGTGAACCAG TTTGCTGCCA AGCTCATTCA GGAGCAGCAC 
CCTGAGGAGG AGCTGATCAA GACAAAGGAG GACGAGGTGA ATGCAGCCTG GCAGCGGCTG 
AAGGGCCTGG CCCTCCAGCG GCAGGGGAAG CTGTTCGGGG CAGCTGAGGT CCAACGCTTT 
AACAGGGATG TGGACGAGAC CATTAGCTGG ATTAAGGAGA AGGAACAGTT AATGGCCTCT 
GATGACTTTG GCCGGGACCT GGCCAGTGTC CAGGCTCTAC TGCGGAAACA CGAGGGTTTG 
GAGAGAGATC TTGCTGCCTT GGAGGACAAG GTCAAAGCCC TGTGTGCCGA GGCTGACCGC 
CTGCAGCAGT CCCACCCTCT GAGTGCCACC CAGATCCAAG TGAAGCGAGA GGAGCTGATC 
ACCAACTGGG AGCAGATCCG CACCCTGGCC GCGGAGAGAC ACGCACGTCT CAACGATTCC 
TACAGGCTTC AGCGCTTCCT TGCTGACTTC CGAGACCTCA CCAGCTGGGT CACTGAGATG 
AAAGCCCTCA TCAATGCAGA TGAACTTGCC AATGATGTGG CCGGGGCCGA AGCCCTGCTG 
GATAGGCATC AGGAGCACAA GGGTGAAATT GACGCTCATG AAGATAGCTT TAAATCTGCA 
GATGAGTCTG GACAGGCCCT GCTTGCAGCT GGTCACTATG CCTCAGATGA AGTGAGGGAG 
AAGCTGACCA TCCTGTCCGA GGAGCGGGCC GCGCTACTAG AGCTCTGGGA GCTGCGACGG 
CAGCAATATG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GCGACACGGA GCAGGTGGAC 
AACTGGATGA GCAAGCAGGA GGCGTTCCTG TTGAACGAAG ACCTGGGTGA TTCCTTGGAT 
AGTGTGGAAG CGCTTCTCAA GAAGCACGAA GACTTTGAGA AGTCCCTCAG CGCCCAGGAG 
GAGAAGATCA CAGCCTTAGA TGAGTTTGCC ACCAAGCTGA TTCAGAACAA CCACTACGCG 
ATGGAAGATG TGGCCACCCG GCGAGACGCA CTGCTGAGCC GCCGCAACGC CCTCCATGAG 
AGAGCCATGT ACCGCCGTGC CCAGCTTGCA GATTCCTTCC ACTTGCAGCA GTTTTTCCGT 
GACTCTGATG AGCTCAAGAG TTGGGTCAAC GAGAAGATGA AAACAGCCAC AGATGAAGCT 
TATAAAGACC CATCCAACCT ACAAGGGAAA GTGCAGAAGC ACCAGGCCTT TGAGGCCGAG 
CTCTCAGCAA ACCAGAGCCG CATCGACGCC TTGGAGAAAG CTGGGCAGAA GCTGATCGAC 
GTTGACCACT ATGCCAAGGA TGAGGTGGCG ACTCGGATGA ATGAAGTTAT CAGTCTGTGG 
AAGAAGCTGC TGGAGGCCAC GGAGCTGAAA GGCATAAAGC TCCGTGAGGC TAACCAGCAG 
CAGCAGTTTA ACCGCAATGT TGAGGACATC GAGCTGTGGC TGTACGAAGT GGAAGGTCAT 
CTGGCCTCGG ATGACTACGG CAAAGACCTC ACCAACGTCC AGAACCTCCA GAAAAAGCAT 
GCCCTGCTGG AGGCTGACGT GGCCGCTCAC CAGGATCGCA TCGATGGCAT CACTATCCAA 
GCCCGCCAGT TCCAGGATGC GGGCCACTTT GATGCTGAAA ACATCAAGAA GAAGCAGGAG 
GCCCTGGTAG CTCGCTACGA GGCACTCAAG GAGCCCATGG TTGCCCGGAA GCAGAAGCTG 
GCCGATTCCC TGCGGTTGCA GCAGCTCTTC CGTGACGTTG AGGATGAGGA GACGTGGATT 
CGGGAGAAAG AACCTATTGC CGCCTCCACC AACAGAGGCA AAGATTTAAT TGGAGTCCAG 
AACCTGCTAA AGAAACACCA AGCGCTACAA GCAGAAATTG CTGGCCACGA GCCCCGCATC 
AAAGCAGTCA CACAGAAGGG GAACGCCATG GTAGAGGAGG GCCATTTTGC TGCCGAGGAC 
GTGAAGGCCA AGCTCCATGA ACTGAACCAG AAGTGGGAGT CGCTGAAGTC CAAGGCCTCC 
CAGCGACGGC AGGACCTGGA GGACTCGCTG CAGGCCCAGC AGTACTTCGC TGATGCCAAC 
GAGGCCGAGT CCTGGATGCG GGAGAAGGAG CCCATCGTGG GCAGCACCGA CTATGGCAAA 
GATGAGGACT CTGCCGAGGC TCTGCTAAAG AAGCACGAGG CATTGATGTC GGATCTCAGC 
GCCTATGGCA GCAGCATCCA GGCCCTGCGA GAGCAGGCCC AGTCGTGCCG GCAACAAGTG 
GCCCCCATGG ATGACGAGAC TGGGAAGGAG CTGGTCTTGG CGCTCTATGA CTATCAGGAG 
AAGAGTCCCC GAGAGGTCAC CATGAAGAAA GGAGACATTC TCACCTTACT CAACAGCACC 
AACAAGGACT GGTGGAAAGT GGAAGTGAAC GACCGGCAGG GCTTCGTGCC GGCCGCCTAC 
GTGAAGAAGC TGGACCCAGC GCAGTCGGCC TCCCGGGAGA ACCTCCTGGA AGAGCAGGGC 
AGCATCGCGC TGCGGCAGGA GCAGATTGAC AACCAGACGC GCGTAACTAA GGAGGCCGGC 
AGCGTGTCTC TGCGTATGAA ACAGGTGGAA GAACTGTACC ATTCTCTGCT GGAACTGGGA 
GAGAAGCGTA AAGGCATGTT GGAGAAGAGC TGTAAGAAGT TCATGCTGTT CCGTGAAGCG 
AACGAGCTGC AGCAGTGGAT CAACGAGAAG GAAGCGGCCC TGACAAGCGA GGAGGTCGGC 
GCGGACTTGG AGCAGGTCGA AGTGCTGCAG AAGAAGTTTG ACGACTTCCA GAAGGATCTC 
AAGGCCAATG AGTCACGGCT GAAGGACATT AACAAGGTGG CTGAAGACCT GGAGTCGGAA 
GGCCTGATGG CGGAGGAGGT GCAGGCCGTG CAGCAGCAGG AGGTGTATGG CGCGATGCCC 
AGGGATGAAT CTGATTCCAA GACAGCCTCC CCGTGGAAGT CTGCTCGCCT GATGGTTCAC 
ACCGTGGCCA CCTTTAATTC CATCAAGGAG CTCAATGAGC GCTGGCGGTC CCTGCAGCAG 
CTGGCTGAGG AGCGGAGCCA ACTCTTGGGC AGCGCCCACG AAGTGCAGCG GTTCCACAGA 
GATGCTGACG AAACCAAAGA GTGGATCGAG GAGAAGAACC AGGCTCTAAA CACAGACAAC 
TATGGCCACG ACCTGGCCAG CGTCCAGGCC CTGCAGCGCA AGCATGAGGG CTTCGAGAGG 
GACCTCGCGG CCCTTGGCGA CAAGGTGAAC TCGCTCGGGG AGACAGCCGA GCGCCTGATC 
CAGTCCCACC CCGAGTCGGC AGAGGACCTG CAGGAGAAGT GCACTGAGCT GAACCAGGCA 
TGGAGCAGCC TGGGCAAGCG TGCCGACCAG CGCAAGGCCA AGCTGGGCGA CTCCCACGAC 
CTGCAGCGCT TCCTCAGCGA CTTCCGGGAC CTCATGTCTT GGATCAATGG AATCCGAGGT 
CTGGTGTCCT CAGACGAGCT AGCCAAGGAT GTCACTGGAG CTGAGGCCCT GCTGGAGCGA 
CACCAGGAAC ACCGGACAGA AATTGATGCC AGAGCTGGCA CTTTCCAGGC ATTCGAGCAG 
TTTGGGCAGC AGCTGTTGGC TCATGGGCAC TACGCCAGCC CCGAGATCAA GGAGAAGCTC 
GATGTTCTAG ACCAGGAGCG CGCAGACCTC GAGAAGGCCT GGGTCCAGCG CAGGATGATG 
CTGGATCAGT GCCTTGAATT GCAGCTGTTC CATCGGGATT GCGAGCAGGC CGAGAACTGG 
ATGGCTGCCC GGGAGGCCTT CCTGAACACA GAAGACAAAG GGGACTCGCT AGACAGCGTG 
GAGGCTCTGA TCAAAAAACA CGAGGACTTT GACAAAGCAA TTAATGTCCA GGAGGAGAAG 
ATTGCTGCCC TGCAGTCCTT TGCTGACCAG CTCATTGCTG GCGGTCACTA CGCCAAAGGC 
GACATCTCCA GCCGGCGCAA TGAGGTTCTG GACAGGTGGC GCCGTCTAAA AGCTCAGATG 
ATTGAGAAGA GGTCAAAGCT GGGAGAATCG CAGACCCTCC AGCAGTTCAG CCGGGATGTA 
GACGAGATTG AAGCTTGGAT CAGTGAAAAA TTACAGACTG CAAGTGATGA GTCATATAAG 
GACCCCACCA ACATCCAGCT TTCCAAGCTG CTGAGCAAGC ATCAGAAGCA CCAGGCCTTC 
GAGGCAGAAC TGCACGCCAA TGCGGACCGG ATCCGCGGGG TCATCGACGT GGGCAACTCC 
CTCATTGACC GTGGGGCCTG TGCCGGCAGC GAGGATGCCG TCAAGGCCCG GCTGGCCGCC 
TTAGCTGACC AGTGGCAGTT CCTGGTGCAG AAGTCGGCTG AGAAGAGCCA GAAGCTGAAA 
GAAGCCAACA AACAGCAGAA CTTCAACACT GGCATCAAGG ACTTCGACTT CTGGCTCTCT 
GAGGTGGAAG CCCTGCTGGC CTCTGAGGAT TATGGCAAAG ACCTGGCTTC TGTGAACAAC 
TTGCTAAAAA AACATCAGCT GCTGGAGGCA GATATCTCTG CCCACGAGGA CCGCCTGAAG 
GACCTCAACA GCCAGGCGGA CAGCCTGATG ACCAGCAGCG CCTTCGACAC CTCCCAGGTG 
AAGGATAAGC GGGACACCAT CAACAGCCGT TTCCAGAAGA TCAAGAGCAT GGCCGCCTCC 
CGGCGAGCTA GGCTGAATGA GTCCCACCGC CTGCACCAGT TCTTCCGGGA CATGGACGAT 
GAGGAGTCCT GGATCAAGGA GAAGAAGCTG CTGGTGAGCT CAGAGGACTA CGGCCGCGAC 
CTGACGGGCG TCCAGAACCT GAGGAAGAAG CACAAACGGC TGGAGGCAGA GCTGGCTGCC 
CACGAGCCGG CCATTCAGGG TGTCCTGGAC ACTGGAAAGA AGCTGTCTGA TGACAACACC 
ATCGGGAAGG AGGAGATTCA GCAGCGCCTG GCGCAGTTTG TGGAGCACTG GAAGGAGCTG 
AAGCAGCTGG CAGCTGCTCG GGGCCAGCGG CTAGAAGAAT CTTTGGAATA TCAGCAGTTT 
GTGGCCAACG TGGAGGAGGA GGAAGCCTGG ATCAATGAGA AGATGACCCT GGTGGCCAGT 
GAGGATTACG GAGACACTCT TGCTGCCATC CAGGGCTTGC TGAAGAAACA CGAAGCCTTC 
GAGACGGACT TCACTGTCCA CAAGGACCGG GTGAATGACG TCTGCACTAA CGGACAAGAC 
CTGGTTAAGA AGAACAATCA CCATGAGGAG AACATCTCTT CCAAGATGAA GGGCTTGAAC 
GGGAAAGTGT CAGACTTGGA GAAGGCTGCC TCCCAGAGGA AGGCAAAGCT CGACGAGAAC 
TCGGCCTTCC TCCAGTTCAA CTGGAAGGCC GATGTGGTGG AGTCCTGGAT TGGTGAAAAG 
GAGAACAGCT TGAAGACAGA TGATTATGGT CGAGATCTGT CTTCTGTCCA AACTCTCCTC 
ACCAAACAGG AAACCTTCGA TGCTGGCCTG CAGGCCTTCC AGCAGGAGGG CATCGCCAAC 
ATCACTGCCC TCAAAGATCA GCTGCTGGCC GCCAAGCATG TTCAGTCCAA GGCCATCGAG 
GCCCGCCACG CTGCCCTCAT GAAGAGATGG AGCCAGCTCC TGGCCAATTC GGCCACCCGG 
AAGAAGAAGC TGCTGGAGGC ACAGAGTCAC TTCCGCAAGG TGGAGGATCT CTTCCTGACC 
TTTGCCAAAA AGGCTTCAGC CTTCAACAGC TGGTTTGAAA ATGCAGAGGA GGACTTAACA 
GACCCAGTGC GCTGCAACTC CCTGGAAGAG ATCAAGGCCT TGAGAGAGGC CCACGATGCC 
TTCCGCTCCT CGCTGAGCTC AGCCCAGGCC GACTTCAACC AGCTGGCTGA GCTGGACCGC 
CAGATCAAGA GCTTCCGCGT GGCCTCCAAC CCCTACACCT GGTTCACCAT GGAGGCCCTG 
GAGGAGACCT GGAGGAACCT TCAGAAGATC ATCAAGGAAA GGGAGCTGGA GCTGCAGAAG 
GAGCAGCGGC GGCAGGAGGA GAACGACAAG CTGCGCCAGG AGTTCGCCCA GCATGCCAAC 
GCCTTCCACC AGTGGATCCA GGAGACCAGA ACGTATCTCC TCGATGGCAT AGCGTATCGT 
CGGGTCATTC GTGTCTATCA GTATGAAGTT GGGGATGATC TGTCTGGAAG GTCCTGCATG 
GTGGAAGAAT CTGGGACCCT TGAATCGCAG CTTGAAGCTA CCAAACGCAA ACACCAGGAG 
ATTCGAGCCA TGAGAAGTCA GCTCAAGAAG ATTGAAGACC TGGGGGCGGC CATGGAGGAA 
GCGCTGATCC TGGACAACAA GTACACGGAG CACAGCACCG TGGGCCTGGC CCAGCAGTGG 
GACCAGCTCG ACCAGCTGGG CATGCGGATG CAGCACAACC TGGAGCAGCA GATCCAGGCC 
AGGAACACGA CAGGAGTGAC CGAGGAGGCC CTCAAAGAAT TCAGCATGAT GTTCAAACAC 
TTCGACAAGG ACAAGTCCGG CCGGCTGAAT CACCAGGAGT TCAAATCCTG TCTGCGCTCC 
CTGGGCTATG ACCTGCCCAT GGTGGAGGAA GGGGAGCCCG ACCCTGAGTT CGAGGCCATC 
TTGGATACTG TGGATCCTAA CAGGGACGGC CACGTCTCCC TGCAAGAGTA CATGGCCTTT 
ATGATCAGCC GTGAGACCGA GAATGTCAAG TCCAGCGAGG AGATTGAGAG CGCCTTCCGG 
GCCCTGAGCT CCGAAGGGAA GCCTTATGTG ACCAAGGAGG AGCTGTACCA GAACCTGACT 
CGGGAACAAG CCGACTACTG CGTCTCCCAC ATGAAGCCCT ACGTGGACGG CAAGGGCCGC 
GAACTCCCCA CTGCTTTCGA CTACGTTGAG TTCACCCGCT CGCTCTTCGT GAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTAN1 ( XM_012147480.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_012002870.1 Ovis aries musimon spectrin, alpha, non-erythrocytic 1 (SPTAN1), transcript variant X5, mRNA.

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CloneID	OOv24451
Clone ID Related Accession (Same CDS sequence)	XM_012147479.2 , XM_012147480.2
Accession Version	XM_012147480.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7497bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	spectrin alpha chain, non-erythrocytic 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942583.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012147480.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCCAA GCGGGGTCAA AGTGCTGGAG ACAGCCGAGG ACATCCAGGA GAGGCGGCAG 
CAGGTCCTGG ACAGATACCA CCGCTTCAAG GAGCTCTCGA CCCTGAGGCG CCAGAAGCTT 
GAAGACTCCT ACCGATTCCA GTTTTTCCAA AGAGATGCTG AGGAGCTGGA GAAATGGATT 
CAGGAGAAGC TTCAGATTGC CTCGGATGAG AATTACAAAG ACCCAACCAA CCTGCAGGGC 
AAGCTGCAGA AGCATCAAGC CTTCGAAGCC GAGGTGCAGG CCAACTCGGG TGCCATCGTG 
AAGCTTGATG AGACCGGTAA CCTGATGATC TCAGAAGGGC ACTTCGCGTC CGAAACCATC 
CGGACTCGTC TGATGGAGCT GCACCGCCAG TGGGAATTGC TTCTGGAGAA GATGCGGGAG 
AAAGGCATCA AGCTGCTGCA GGCCCAGAAG CTGGTGCAGT ACCTGCGGGA GTGTGAGGAC 
GTGATGGACT GGATCAATGA CAAGGAAGCC ATTGTGACAT CTGAGGAGCT GGGCCAGGAC 
CTGGAGCACG TGGAGGTGTT ACAGAAGAAA TTCGAAGAGT TTCAGACAGA CATGGCTGCC 
CACGAAGAGA GAGTCAATGA AGTGAACCAG TTTGCTGCCA AGCTCATTCA GGAGCAGCAC 
CCTGAGGAGG AGCTGATCAA GACAAAGGAG GACGAGGTGA ATGCAGCCTG GCAGCGGCTG 
AAGGGCCTGG CCCTCCAGCG GCAGGGGAAG CTGTTCGGGG CAGCTGAGGT CCAACGCTTT 
AACAGGGATG TGGACGAGAC CATTAGCTGG ATTAAGGAGA AGGAACAGTT AATGGCCTCT 
GATGACTTTG GCCGGGACCT GGCCAGTGTC CAGGCTCTAC TGCGGAAACA CGAGGGTTTG 
GAGAGAGATC TTGCTGCCTT GGAGGACAAG GTCAAAGCCC TGTGTGCCGA GGCTGACCGC 
CTGCAGCAGT CCCACCCTCT GAGTGCCACC CAGATCCAAG TGAAGCGAGA GGAGCTGATC 
ACCAACTGGG AGCAGATCCG CACCCTGGCC GCGGAGAGAC ACGCACGTCT CAACGATTCC 
TACAGGCTTC AGCGCTTCCT TGCTGACTTC CGAGACCTCA CCAGCTGGGT CACTGAGATG 
AAAGCCCTCA TCAATGCAGA TGAACTTGCC AATGATGTGG CCGGGGCCGA AGCCCTGCTG 
GATAGGCATC AGGAGCACAA GGGTGAAATT GACGCTCATG AAGATAGCTT TAAATCTGCA 
GATGAGTCTG GACAGGCCCT GCTTGCAGCT GGTCACTATG CCTCAGATGA AGTGAGGGAG 
AAGCTGACCA TCCTGTCCGA GGAGCGGGCC GCGCTACTAG AGCTCTGGGA GCTGCGACGG 
CAGCAATATG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GCGACACGGA GCAGGTGGAC 
AACTGGATGA GCAAGCAGGA GGCGTTCCTG TTGAACGAAG ACCTGGGTGA TTCCTTGGAT 
AGTGTGGAAG CGCTTCTCAA GAAGCACGAA GACTTTGAGA AGTCCCTCAG CGCCCAGGAG 
GAGAAGATCA CAGCCTTAGA TGAGTTTGCC ACCAAGCTGA TTCAGAACAA CCACTACGCG 
ATGGAAGATG TGGCCACCCG GCGAGACGCA CTGCTGAGCC GCCGCAACGC CCTCCATGAG 
AGAGCCATGT ACCGCCGTGC CCAGCTTGCA GATTCCTTCC ACTTGCAGCA GTTTTTCCGT 
GACTCTGATG AGCTCAAGAG TTGGGTCAAC GAGAAGATGA AAACAGCCAC AGATGAAGCT 
TATAAAGACC CATCCAACCT ACAAGGGAAA GTGCAGAAGC ACCAGGCCTT TGAGGCCGAG 
CTCTCAGCAA ACCAGAGCCG CATCGACGCC TTGGAGAAAG CTGGGCAGAA GCTGATCGAC 
GTTGACCACT ATGCCAAGGA TGAGGTGGCG ACTCGGATGA ATGAAGTTAT CAGTCTGTGG 
AAGAAGCTGC TGGAGGCCAC GGAGCTGAAA GGCATAAAGC TCCGTGAGGC TAACCAGCAG 
CAGCAGTTTA ACCGCAATGT TGAGGACATC GAGCTGTGGC TGTACGAAGT GGAAGGTCAT 
CTGGCCTCGG ATGACTACGG CAAAGACCTC ACCAACGTCC AGAACCTCCA GAAAAAGCAT 
GCCCTGCTGG AGGCTGACGT GGCCGCTCAC CAGGATCGCA TCGATGGCAT CACTATCCAA 
GCCCGCCAGT TCCAGGATGC GGGCCACTTT GATGCTGAAA ACATCAAGAA GAAGCAGGAG 
GCCCTGGTAG CTCGCTACGA GGCACTCAAG GAGCCCATGG TTGCCCGGAA GCAGAAGCTG 
GCCGATTCCC TGCGGTTGCA GCAGCTCTTC CGTGACGTTG AGGATGAGGA GACGTGGATT 
CGGGAGAAAG AACCTATTGC CGCCTCCACC AACAGAGGCA AAGATTTAAT TGGAGTCCAG 
AACCTGCTAA AGAAACACCA AGCGCTACAA GCAGAAATTG CTGGCCACGA GCCCCGCATC 
AAAGCAGTCA CACAGAAGGG GAACGCCATG GTAGAGGAGG GCCATTTTGC TGCCGAGGAC 
GTGAAGGCCA AGCTCCATGA ACTGAACCAG AAGTGGGAGT CGCTGAAGTC CAAGGCCTCC 
CAGCGACGGC AGGACCTGGA GGACTCGCTG CAGGCCCAGC AGTACTTCGC TGATGCCAAC 
GAGGCCGAGT CCTGGATGCG GGAGAAGGAG CCCATCGTGG GCAGCACCGA CTATGGCAAA 
GATGAGGACT CTGCCGAGGC TCTGCTAAAG AAGCACGAGG CATTGATGTC GGATCTCAGC 
GCCTATGGCA GCAGCATCCA GGCCCTGCGA GAGCAGGCCC AGTCGTGCCG GCAACAAGTG 
GCCCCCATGG ATGACGAGAC TGGGAAGGAG CTGGTCTTGG CGCTCTATGA CTATCAGGAG 
AAGAGTCCCC GAGAGGTCAC CATGAAGAAA GGAGACATTC TCACCTTACT CAACAGCACC 
AACAAGGACT GGTGGAAAGT GGAAGTGAAC GACCGGCAGG GCTTCGTGCC GGCCGCCTAC 
GTGAAGAAGC TGGACCCAGC GCAGTCGGCC TCCCGGGAGA ACCTCCTGGA AGAGCAGGGC 
AGCATCGCGC TGCGGCAGGA GCAGATTGAC AACCAGACGC GCGTAACTAA GGAGGCCGGC 
AGCGTGTCTC TGCGTATGAA ACAGGTGGAA GAACTGTACC ATTCTCTGCT GGAACTGGGA 
GAGAAGCGTA AAGGCATGTT GGAGAAGAGC TGTAAGAAGT TCATGCTGTT CCGTGAAGCG 
AACGAGCTGC AGCAGTGGAT CAACGAGAAG GAAGCGGCCC TGACAAGCGA GGAGGTCGGC 
GCGGACTTGG AGCAGGTCGA AGTGCTGCAG AAGAAGTTTG ACGACTTCCA GAAGGATCTC 
AAGGCCAATG AGTCACGGCT GAAGGACATT AACAAGGTGG CTGAAGACCT GGAGTCGGAA 
GGCCTGATGG CGGAGGAGGT GCAGGCCGTG CAGCAGCAGG AGGTGTATGG CGCGATGCCC 
AGGGATGAAT CTGATTCCAA GACAGCCTCC CCGTGGAAGT CTGCTCGCCT GATGGTTCAC 
ACCGTGGCCA CCTTTAATTC CATCAAGGAG CTCAATGAGC GCTGGCGGTC CCTGCAGCAG 
CTGGCTGAGG AGCGGAGCCA ACTCTTGGGC AGCGCCCACG AAGTGCAGCG GTTCCACAGA 
GATGCTGACG AAACCAAAGA GTGGATCGAG GAGAAGAACC AGGCTCTAAA CACAGACAAC 
TATGGCCACG ACCTGGCCAG CGTCCAGGCC CTGCAGCGCA AGCATGAGGG CTTCGAGAGG 
GACCTCGCGG CCCTTGGCGA CAAGGTGAAC TCGCTCGGGG AGACAGCCGA GCGCCTGATC 
CAGTCCCACC CCGAGTCGGC AGAGGACCTG CAGGAGAAGT GCACTGAGCT GAACCAGGCA 
TGGAGCAGCC TGGGCAAGCG TGCCGACCAG CGCAAGGCCA AGCTGGGCGA CTCCCACGAC 
CTGCAGCGCT TCCTCAGCGA CTTCCGGGAC CTCATGTCTT GGATCAATGG AATCCGAGGT 
CTGGTGTCCT CAGACGAGCT AGCCAAGGAT GTCACTGGAG CTGAGGCCCT GCTGGAGCGA 
CACCAGGAAC ACCGGACAGA AATTGATGCC AGAGCTGGCA CTTTCCAGGC ATTCGAGCAG 
TTTGGGCAGC AGCTGTTGGC TCATGGGCAC TACGCCAGCC CCGAGATCAA GGAGAAGCTC 
GATGTTCTAG ACCAGGAGCG CGCAGACCTC GAGAAGGCCT GGGTCCAGCG CAGGATGATG 
CTGGATCAGT GCCTTGAATT GCAGCTGTTC CATCGGGATT GCGAGCAGGC CGAGAACTGG 
ATGGCTGCCC GGGAGGCCTT CCTGAACACA GAAGACAAAG GGGACTCGCT AGACAGCGTG 
GAGGCTCTGA TCAAAAAACA CGAGGACTTT GACAAAGCAA TTAATGTCCA GGAGGAGAAG 
ATTGCTGCCC TGCAGTCCTT TGCTGACCAG CTCATTGCTG GCGGTCACTA CGCCAAAGGC 
GACATCTCCA GCCGGCGCAA TGAGGTTCTG GACAGGTGGC GCCGTCTAAA AGCTCAGATG 
ATTGAGAAGA GGTCAAAGCT GGGAGAATCG CAGACCCTCC AGCAGTTCAG CCGGGATGTA 
GACGAGATTG AAGCTTGGAT CAGTGAAAAA TTACAGACTG CAAGTGATGA GTCATATAAG 
GACCCCACCA ACATCCAGCT TTCCAAGCTG CTGAGCAAGC ATCAGAAGCA CCAGGCCTTC 
GAGGCAGAAC TGCACGCCAA TGCGGACCGG ATCCGCGGGG TCATCGACGT GGGCAACTCC 
CTCATTGACC GTGGGGCCTG TGCCGGCAGC GAGGATGCCG TCAAGGCCCG GCTGGCCGCC 
TTAGCTGACC AGTGGCAGTT CCTGGTGCAG AAGTCGGCTG AGAAGAGCCA GAAGCTGAAA 
GAAGCCAACA AACAGCAGAA CTTCAACACT GGCATCAAGG ACTTCGACTT CTGGCTCTCT 
GAGGTGGAAG CCCTGCTGGC CTCTGAGGAT TATGGCAAAG ACCTGGCTTC TGTGAACAAC 
TTGCTAAAAA AACATCAGCT GCTGGAGGCA GATATCTCTG CCCACGAGGA CCGCCTGAAG 
GACCTCAACA GCCAGGCGGA CAGCCTGATG ACCAGCAGCG CCTTCGACAC CTCCCAGGTG 
AAGGATAAGC GGGACACCAT CAACAGCCGT TTCCAGAAGA TCAAGAGCAT GGCCGCCTCC 
CGGCGAGCTA GGCTGAATGA GTCCCACCGC CTGCACCAGT TCTTCCGGGA CATGGACGAT 
GAGGAGTCCT GGATCAAGGA GAAGAAGCTG CTGGTGAGCT CAGAGGACTA CGGCCGCGAC 
CTGACGGGCG TCCAGAACCT GAGGAAGAAG CACAAACGGC TGGAGGCAGA GCTGGCTGCC 
CACGAGCCGG CCATTCAGGG TGTCCTGGAC ACTGGAAAGA AGCTGTCTGA TGACAACACC 
ATCGGGAAGG AGGAGATTCA GCAGCGCCTG GCGCAGTTTG TGGAGCACTG GAAGGAGCTG 
AAGCAGCTGG CAGCTGCTCG GGGCCAGCGG CTAGAAGAAT CTTTGGAATA TCAGCAGTTT 
GTGGCCAACG TGGAGGAGGA GGAAGCCTGG ATCAATGAGA AGATGACCCT GGTGGCCAGT 
GAGGATTACG GAGACACTCT TGCTGCCATC CAGGGCTTGC TGAAGAAACA CGAAGCCTTC 
GAGACGGACT TCACTGTCCA CAAGGACCGG GTGAATGACG TCTGCACTAA CGGACAAGAC 
CTGGTTAAGA AGAACAATCA CCATGAGGAG AACATCTCTT CCAAGATGAA GGGCTTGAAC 
GGGAAAGTGT CAGACTTGGA GAAGGCTGCC TCCCAGAGGA AGGCAAAGCT CGACGAGAAC 
TCGGCCTTCC TCCAGTTCAA CTGGAAGGCC GATGTGGTGG AGTCCTGGAT TGGTGAAAAG 
GAGAACAGCT TGAAGACAGA TGATTATGGT CGAGATCTGT CTTCTGTCCA AACTCTCCTC 
ACCAAACAGG AAACCTTCGA TGCTGGCCTG CAGGCCTTCC AGCAGGAGGG CATCGCCAAC 
ATCACTGCCC TCAAAGATCA GCTGCTGGCC GCCAAGCATG TTCAGTCCAA GGCCATCGAG 
GCCCGCCACG CTGCCCTCAT GAAGAGATGG AGCCAGCTCC TGGCCAATTC GGCCACCCGG 
AAGAAGAAGC TGCTGGAGGC ACAGAGTCAC TTCCGCAAGG TGGAGGATCT CTTCCTGACC 
TTTGCCAAAA AGGCTTCAGC CTTCAACAGC TGGTTTGAAA ATGCAGAGGA GGACTTAACA 
GACCCAGTGC GCTGCAACTC CCTGGAAGAG ATCAAGGCCT TGAGAGAGGC CCACGATGCC 
TTCCGCTCCT CGCTGAGCTC AGCCCAGGCC GACTTCAACC AGCTGGCTGA GCTGGACCGC 
CAGATCAAGA GCTTCCGCGT GGCCTCCAAC CCCTACACCT GGTTCACCAT GGAGGCCCTG 
GAGGAGACCT GGAGGAACCT TCAGAAGATC ATCAAGGAAA GGGAGCTGGA GCTGCAGAAG 
GAGCAGCGGC GGCAGGAGGA GAACGACAAG CTGCGCCAGG AGTTCGCCCA GCATGCCAAC 
GCCTTCCACC AGTGGATCCA GGAGACCAGA ACGTATCTCC TCGATGGCAT AGCGTATCGT 
CGGGTCATTC GTGTCTATCA GTATGAAGTT GGGGATGATC TGTCTGGAAG GTCCTGCATG 
GTGGAAGAAT CTGGGACCCT TGAATCGCAG CTTGAAGCTA CCAAACGCAA ACACCAGGAG 
ATTCGAGCCA TGAGAAGTCA GCTCAAGAAG ATTGAAGACC TGGGGGCGGC CATGGAGGAA 
GCGCTGATCC TGGACAACAA GTACACGGAG CACAGCACCG TGGGCCTGGC CCAGCAGTGG 
GACCAGCTCG ACCAGCTGGG CATGCGGATG CAGCACAACC TGGAGCAGCA GATCCAGGCC 
AGGAACACGA CAGGAGTGAC CGAGGAGGCC CTCAAAGAAT TCAGCATGAT GTTCAAACAC 
TTCGACAAGG ACAAGTCCGG CCGGCTGAAT CACCAGGAGT TCAAATCCTG TCTGCGCTCC 
CTGGGCTATG ACCTGCCCAT GGTGGAGGAA GGGGAGCCCG ACCCTGAGTT CGAGGCCATC 
TTGGATACTG TGGATCCTAA CAGGGACGGC CACGTCTCCC TGCAAGAGTA CATGGCCTTT 
ATGATCAGCC GTGAGACCGA GAATGTCAAG TCCAGCGAGG AGATTGAGAG CGCCTTCCGG 
GCCCTGAGCT CCGAAGGGAA GCCTTATGTG ACCAAGGAGG AGCTGTACCA GAACCTGACT 
CGGGAACAAG CCGACTACTG CGTCTCCCAC ATGAAGCCCT ACGTGGACGG CAAGGGCCGC 
GAACTCCCCA CTGCTTTCGA CTACGTTGAG TTCACCCGCT CGCTCTTCGT GAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012002870.1
CDS	125..7621
Translation	MDPSGVKVLETAEDIQERRQQVLDRYHRFKELSTLRRQKLEDSYRFQFFQRDAEELEKWIQEKLQIASDENYKDPTNLQGKLQKHQAFEAEVQANSGAIVKLDETGNLMISEGHFASETIRTRLMELHRQWELLLEKMREKGIKLLQAQKLVQYLRECEDVMDWINDKEAIVTSEELGQDLEHVEVLQKKFEEFQTDMAAHEERVNEVNQFAAKLIQEQHPEEELIKTKEDEVNAAWQRLKGLALQRQGKLFGAAEVQRFNRDVDETISWIKEKEQLMASDDFGRDLASVQALLRKHEGLERDLAALEDKVKALCAEADRLQQSHPLSATQIQVKREELITNWEQIRTLAAERHARLNDSYRLQRFLADFRDLTSWVTEMKALINADELANDVAGAEALLDRHQEHKGEIDAHEDSFKSADESGQALLAAGHYASDEVREKLTILSEERAALLELWELRRQQYEQCMDLQLFYRDTEQVDNWMSKQEAFLLNEDLGDSLDSVEALLKKHEDFEKSLSAQEEKITALDEFATKLIQNNHYAMEDVATRRDALLSRRNALHERAMYRRAQLADSFHLQQFFRDSDELKSWVNEKMKTATDEAYKDPSNLQGKVQKHQAFEAELSANQSRIDALEKAGQKLIDVDHYAKDEVATRMNEVISLWKKLLEATELKGIKLREANQQQQFNRNVEDIELWLYEVEGHLASDDYGKDLTNVQNLQKKHALLEADVAAHQDRIDGITIQARQFQDAGHFDAENIKKKQEALVARYEALKEPMVARKQKLADSLRLQQLFRDVEDEETWIREKEPIAASTNRGKDLIGVQNLLKKHQALQAEIAGHEPRIKAVTQKGNAMVEEGHFAAEDVKAKLHELNQKWESLKSKASQRRQDLEDSLQAQQYFADANEAESWMREKEPIVGSTDYGKDEDSAEALLKKHEALMSDLSAYGSSIQALREQAQSCRQQVAPMDDETGKELVLALYDYQEKSPREVTMKKGDILTLLNSTNKDWWKVEVNDRQGFVPAAYVKKLDPAQSASRENLLEEQGSIALRQEQIDNQTRVTKEAGSVSLRMKQVEELYHSLLELGEKRKGMLEKSCKKFMLFREANELQQWINEKEAALTSEEVGADLEQVEVLQKKFDDFQKDLKANESRLKDINKVAEDLESEGLMAEEVQAVQQQEVYGAMPRDESDSKTASPWKSARLMVHTVATFNSIKELNERWRSLQQLAEERSQLLGSAHEVQRFHRDADETKEWIEEKNQALNTDNYGHDLASVQALQRKHEGFERDLAALGDKVNSLGETAERLIQSHPESAEDLQEKCTELNQAWSSLGKRADQRKAKLGDSHDLQRFLSDFRDLMSWINGIRGLVSSDELAKDVTGAEALLERHQEHRTEIDARAGTFQAFEQFGQQLLAHGHYASPEIKEKLDVLDQERADLEKAWVQRRMMLDQCLELQLFHRDCEQAENWMAAREAFLNTEDKGDSLDSVEALIKKHEDFDKAINVQEEKIAALQSFADQLIAGGHYAKGDISSRRNEVLDRWRRLKAQMIEKRSKLGESQTLQQFSRDVDEIEAWISEKLQTASDESYKDPTNIQLSKLLSKHQKHQAFEAELHANADRIRGVIDVGNSLIDRGACAGSEDAVKARLAALADQWQFLVQKSAEKSQKLKEANKQQNFNTGIKDFDFWLSEVEALLASEDYGKDLASVNNLLKKHQLLEADISAHEDRLKDLNSQADSLMTSSAFDTSQVKDKRDTINSRFQKIKSMAASRRARLNESHRLHQFFRDMDDEESWIKEKKLLVSSEDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQFVEHWKELKQLAAARGQRLEESLEYQQFVANVEEEEAWINEKMTLVASEDYGDTLAAIQGLLKKHEAFETDFTVHKDRVNDVCTNGQDLVKKNNHHEENISSKMKGLNGKVSDLEKAASQRKAKLDENSAFLQFNWKADVVESWIGEKENSLKTDDYGRDLSSVQTLLTKQETFDAGLQAFQQEGIANITALKDQLLAAKHVQSKAIEARHAALMKRWSQLLANSATRKKKLLEAQSHFRKVEDLFLTFAKKASAFNSWFENAEEDLTDPVRCNSLEEIKALREAHDAFRSSLSSAQADFNQLAELDRQIKSFRVASNPYTWFTMEALEETWRNLQKIIKERELELQKEQRRQEENDKLRQEFAQHANAFHQWIQETRTYLLDGIAYRRVIRVYQYEVGDDLSGRSCMVEESGTLESQLEATKRKHQEIRAMRSQLKKIEDLGAAMEEALILDNKYTEHSTVGLAQQWDQLDQLGMRMQHNLEQQIQARNTTGVTEEALKEFSMMFKHFDKDKSGRLNHQEFKSCLRSLGYDLPMVEEGEPDPEFEAILDTVDPNRDGHVSLQEYMAFMISRETENVKSSEEIESAFRALSSEGKPYVTKEELYQNLTREQADYCVSHMKPYVDGKGRELPTAFDYVEFTRSLFVN

Target ORF information:

RefSeq Version	XM_012147480.2
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon spectrin, alpha, non-erythrocytic 1 (SPTAN1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012147480.2

ORF Insert Sequence:

ATGGACCCAA GCGGGGTCAA AGTGCTGGAG ACAGCCGAGG ACATCCAGGA GAGGCGGCAG 
CAGGTCCTGG ACAGATACCA CCGCTTCAAG GAGCTCTCGA CCCTGAGGCG CCAGAAGCTT 
GAAGACTCCT ACCGATTCCA GTTTTTCCAA AGAGATGCTG AGGAGCTGGA GAAATGGATT 
CAGGAGAAGC TTCAGATTGC CTCGGATGAG AATTACAAAG ACCCAACCAA CCTGCAGGGC 
AAGCTGCAGA AGCATCAAGC CTTCGAAGCC GAGGTGCAGG CCAACTCGGG TGCCATCGTG 
AAGCTTGATG AGACCGGTAA CCTGATGATC TCAGAAGGGC ACTTCGCGTC CGAAACCATC 
CGGACTCGTC TGATGGAGCT GCACCGCCAG TGGGAATTGC TTCTGGAGAA GATGCGGGAG 
AAAGGCATCA AGCTGCTGCA GGCCCAGAAG CTGGTGCAGT ACCTGCGGGA GTGTGAGGAC 
GTGATGGACT GGATCAATGA CAAGGAAGCC ATTGTGACAT CTGAGGAGCT GGGCCAGGAC 
CTGGAGCACG TGGAGGTGTT ACAGAAGAAA TTCGAAGAGT TTCAGACAGA CATGGCTGCC 
CACGAAGAGA GAGTCAATGA AGTGAACCAG TTTGCTGCCA AGCTCATTCA GGAGCAGCAC 
CCTGAGGAGG AGCTGATCAA GACAAAGGAG GACGAGGTGA ATGCAGCCTG GCAGCGGCTG 
AAGGGCCTGG CCCTCCAGCG GCAGGGGAAG CTGTTCGGGG CAGCTGAGGT CCAACGCTTT 
AACAGGGATG TGGACGAGAC CATTAGCTGG ATTAAGGAGA AGGAACAGTT AATGGCCTCT 
GATGACTTTG GCCGGGACCT GGCCAGTGTC CAGGCTCTAC TGCGGAAACA CGAGGGTTTG 
GAGAGAGATC TTGCTGCCTT GGAGGACAAG GTCAAAGCCC TGTGTGCCGA GGCTGACCGC 
CTGCAGCAGT CCCACCCTCT GAGTGCCACC CAGATCCAAG TGAAGCGAGA GGAGCTGATC 
ACCAACTGGG AGCAGATCCG CACCCTGGCC GCGGAGAGAC ACGCACGTCT CAACGATTCC 
TACAGGCTTC AGCGCTTCCT TGCTGACTTC CGAGACCTCA CCAGCTGGGT CACTGAGATG 
AAAGCCCTCA TCAATGCAGA TGAACTTGCC AATGATGTGG CCGGGGCCGA AGCCCTGCTG 
GATAGGCATC AGGAGCACAA GGGTGAAATT GACGCTCATG AAGATAGCTT TAAATCTGCA 
GATGAGTCTG GACAGGCCCT GCTTGCAGCT GGTCACTATG CCTCAGATGA AGTGAGGGAG 
AAGCTGACCA TCCTGTCCGA GGAGCGGGCC GCGCTACTAG AGCTCTGGGA GCTGCGACGG 
CAGCAATATG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GCGACACGGA GCAGGTGGAC 
AACTGGATGA GCAAGCAGGA GGCGTTCCTG TTGAACGAAG ACCTGGGTGA TTCCTTGGAT 
AGTGTGGAAG CGCTTCTCAA GAAGCACGAA GACTTTGAGA AGTCCCTCAG CGCCCAGGAG 
GAGAAGATCA CAGCCTTAGA TGAGTTTGCC ACCAAGCTGA TTCAGAACAA CCACTACGCG 
ATGGAAGATG TGGCCACCCG GCGAGACGCA CTGCTGAGCC GCCGCAACGC CCTCCATGAG 
AGAGCCATGT ACCGCCGTGC CCAGCTTGCA GATTCCTTCC ACTTGCAGCA GTTTTTCCGT 
GACTCTGATG AGCTCAAGAG TTGGGTCAAC GAGAAGATGA AAACAGCCAC AGATGAAGCT 
TATAAAGACC CATCCAACCT ACAAGGGAAA GTGCAGAAGC ACCAGGCCTT TGAGGCCGAG 
CTCTCAGCAA ACCAGAGCCG CATCGACGCC TTGGAGAAAG CTGGGCAGAA GCTGATCGAC 
GTTGACCACT ATGCCAAGGA TGAGGTGGCG ACTCGGATGA ATGAAGTTAT CAGTCTGTGG 
AAGAAGCTGC TGGAGGCCAC GGAGCTGAAA GGCATAAAGC TCCGTGAGGC TAACCAGCAG 
CAGCAGTTTA ACCGCAATGT TGAGGACATC GAGCTGTGGC TGTACGAAGT GGAAGGTCAT 
CTGGCCTCGG ATGACTACGG CAAAGACCTC ACCAACGTCC AGAACCTCCA GAAAAAGCAT 
GCCCTGCTGG AGGCTGACGT GGCCGCTCAC CAGGATCGCA TCGATGGCAT CACTATCCAA 
GCCCGCCAGT TCCAGGATGC GGGCCACTTT GATGCTGAAA ACATCAAGAA GAAGCAGGAG 
GCCCTGGTAG CTCGCTACGA GGCACTCAAG GAGCCCATGG TTGCCCGGAA GCAGAAGCTG 
GCCGATTCCC TGCGGTTGCA GCAGCTCTTC CGTGACGTTG AGGATGAGGA GACGTGGATT 
CGGGAGAAAG AACCTATTGC CGCCTCCACC AACAGAGGCA AAGATTTAAT TGGAGTCCAG 
AACCTGCTAA AGAAACACCA AGCGCTACAA GCAGAAATTG CTGGCCACGA GCCCCGCATC 
AAAGCAGTCA CACAGAAGGG GAACGCCATG GTAGAGGAGG GCCATTTTGC TGCCGAGGAC 
GTGAAGGCCA AGCTCCATGA ACTGAACCAG AAGTGGGAGT CGCTGAAGTC CAAGGCCTCC 
CAGCGACGGC AGGACCTGGA GGACTCGCTG CAGGCCCAGC AGTACTTCGC TGATGCCAAC 
GAGGCCGAGT CCTGGATGCG GGAGAAGGAG CCCATCGTGG GCAGCACCGA CTATGGCAAA 
GATGAGGACT CTGCCGAGGC TCTGCTAAAG AAGCACGAGG CATTGATGTC GGATCTCAGC 
GCCTATGGCA GCAGCATCCA GGCCCTGCGA GAGCAGGCCC AGTCGTGCCG GCAACAAGTG 
GCCCCCATGG ATGACGAGAC TGGGAAGGAG CTGGTCTTGG CGCTCTATGA CTATCAGGAG 
AAGAGTCCCC GAGAGGTCAC CATGAAGAAA GGAGACATTC TCACCTTACT CAACAGCACC 
AACAAGGACT GGTGGAAAGT GGAAGTGAAC GACCGGCAGG GCTTCGTGCC GGCCGCCTAC 
GTGAAGAAGC TGGACCCAGC GCAGTCGGCC TCCCGGGAGA ACCTCCTGGA AGAGCAGGGC 
AGCATCGCGC TGCGGCAGGA GCAGATTGAC AACCAGACGC GCGTAACTAA GGAGGCCGGC 
AGCGTGTCTC TGCGTATGAA ACAGGTGGAA GAACTGTACC ATTCTCTGCT GGAACTGGGA 
GAGAAGCGTA AAGGCATGTT GGAGAAGAGC TGTAAGAAGT TCATGCTGTT CCGTGAAGCG 
AACGAGCTGC AGCAGTGGAT CAACGAGAAG GAAGCGGCCC TGACAAGCGA GGAGGTCGGC 
GCGGACTTGG AGCAGGTCGA AGTGCTGCAG AAGAAGTTTG ACGACTTCCA GAAGGATCTC 
AAGGCCAATG AGTCACGGCT GAAGGACATT AACAAGGTGG CTGAAGACCT GGAGTCGGAA 
GGCCTGATGG CGGAGGAGGT GCAGGCCGTG CAGCAGCAGG AGGTGTATGG CGCGATGCCC 
AGGGATGAAT CTGATTCCAA GACAGCCTCC CCGTGGAAGT CTGCTCGCCT GATGGTTCAC 
ACCGTGGCCA CCTTTAATTC CATCAAGGAG CTCAATGAGC GCTGGCGGTC CCTGCAGCAG 
CTGGCTGAGG AGCGGAGCCA ACTCTTGGGC AGCGCCCACG AAGTGCAGCG GTTCCACAGA 
GATGCTGACG AAACCAAAGA GTGGATCGAG GAGAAGAACC AGGCTCTAAA CACAGACAAC 
TATGGCCACG ACCTGGCCAG CGTCCAGGCC CTGCAGCGCA AGCATGAGGG CTTCGAGAGG 
GACCTCGCGG CCCTTGGCGA CAAGGTGAAC TCGCTCGGGG AGACAGCCGA GCGCCTGATC 
CAGTCCCACC CCGAGTCGGC AGAGGACCTG CAGGAGAAGT GCACTGAGCT GAACCAGGCA 
TGGAGCAGCC TGGGCAAGCG TGCCGACCAG CGCAAGGCCA AGCTGGGCGA CTCCCACGAC 
CTGCAGCGCT TCCTCAGCGA CTTCCGGGAC CTCATGTCTT GGATCAATGG AATCCGAGGT 
CTGGTGTCCT CAGACGAGCT AGCCAAGGAT GTCACTGGAG CTGAGGCCCT GCTGGAGCGA 
CACCAGGAAC ACCGGACAGA AATTGATGCC AGAGCTGGCA CTTTCCAGGC ATTCGAGCAG 
TTTGGGCAGC AGCTGTTGGC TCATGGGCAC TACGCCAGCC CCGAGATCAA GGAGAAGCTC 
GATGTTCTAG ACCAGGAGCG CGCAGACCTC GAGAAGGCCT GGGTCCAGCG CAGGATGATG 
CTGGATCAGT GCCTTGAATT GCAGCTGTTC CATCGGGATT GCGAGCAGGC CGAGAACTGG 
ATGGCTGCCC GGGAGGCCTT CCTGAACACA GAAGACAAAG GGGACTCGCT AGACAGCGTG 
GAGGCTCTGA TCAAAAAACA CGAGGACTTT GACAAAGCAA TTAATGTCCA GGAGGAGAAG 
ATTGCTGCCC TGCAGTCCTT TGCTGACCAG CTCATTGCTG GCGGTCACTA CGCCAAAGGC 
GACATCTCCA GCCGGCGCAA TGAGGTTCTG GACAGGTGGC GCCGTCTAAA AGCTCAGATG 
ATTGAGAAGA GGTCAAAGCT GGGAGAATCG CAGACCCTCC AGCAGTTCAG CCGGGATGTA 
GACGAGATTG AAGCTTGGAT CAGTGAAAAA TTACAGACTG CAAGTGATGA GTCATATAAG 
GACCCCACCA ACATCCAGCT TTCCAAGCTG CTGAGCAAGC ATCAGAAGCA CCAGGCCTTC 
GAGGCAGAAC TGCACGCCAA TGCGGACCGG ATCCGCGGGG TCATCGACGT GGGCAACTCC 
CTCATTGACC GTGGGGCCTG TGCCGGCAGC GAGGATGCCG TCAAGGCCCG GCTGGCCGCC 
TTAGCTGACC AGTGGCAGTT CCTGGTGCAG AAGTCGGCTG AGAAGAGCCA GAAGCTGAAA 
GAAGCCAACA AACAGCAGAA CTTCAACACT GGCATCAAGG ACTTCGACTT CTGGCTCTCT 
GAGGTGGAAG CCCTGCTGGC CTCTGAGGAT TATGGCAAAG ACCTGGCTTC TGTGAACAAC 
TTGCTAAAAA AACATCAGCT GCTGGAGGCA GATATCTCTG CCCACGAGGA CCGCCTGAAG 
GACCTCAACA GCCAGGCGGA CAGCCTGATG ACCAGCAGCG CCTTCGACAC CTCCCAGGTG 
AAGGATAAGC GGGACACCAT CAACAGCCGT TTCCAGAAGA TCAAGAGCAT GGCCGCCTCC 
CGGCGAGCTA GGCTGAATGA GTCCCACCGC CTGCACCAGT TCTTCCGGGA CATGGACGAT 
GAGGAGTCCT GGATCAAGGA GAAGAAGCTG CTGGTGAGCT CAGAGGACTA CGGCCGCGAC 
CTGACGGGCG TCCAGAACCT GAGGAAGAAG CACAAACGGC TGGAGGCAGA GCTGGCTGCC 
CACGAGCCGG CCATTCAGGG TGTCCTGGAC ACTGGAAAGA AGCTGTCTGA TGACAACACC 
ATCGGGAAGG AGGAGATTCA GCAGCGCCTG GCGCAGTTTG TGGAGCACTG GAAGGAGCTG 
AAGCAGCTGG CAGCTGCTCG GGGCCAGCGG CTAGAAGAAT CTTTGGAATA TCAGCAGTTT 
GTGGCCAACG TGGAGGAGGA GGAAGCCTGG ATCAATGAGA AGATGACCCT GGTGGCCAGT 
GAGGATTACG GAGACACTCT TGCTGCCATC CAGGGCTTGC TGAAGAAACA CGAAGCCTTC 
GAGACGGACT TCACTGTCCA CAAGGACCGG GTGAATGACG TCTGCACTAA CGGACAAGAC 
CTGGTTAAGA AGAACAATCA CCATGAGGAG AACATCTCTT CCAAGATGAA GGGCTTGAAC 
GGGAAAGTGT CAGACTTGGA GAAGGCTGCC TCCCAGAGGA AGGCAAAGCT CGACGAGAAC 
TCGGCCTTCC TCCAGTTCAA CTGGAAGGCC GATGTGGTGG AGTCCTGGAT TGGTGAAAAG 
GAGAACAGCT TGAAGACAGA TGATTATGGT CGAGATCTGT CTTCTGTCCA AACTCTCCTC 
ACCAAACAGG AAACCTTCGA TGCTGGCCTG CAGGCCTTCC AGCAGGAGGG CATCGCCAAC 
ATCACTGCCC TCAAAGATCA GCTGCTGGCC GCCAAGCATG TTCAGTCCAA GGCCATCGAG 
GCCCGCCACG CTGCCCTCAT GAAGAGATGG AGCCAGCTCC TGGCCAATTC GGCCACCCGG 
AAGAAGAAGC TGCTGGAGGC ACAGAGTCAC TTCCGCAAGG TGGAGGATCT CTTCCTGACC 
TTTGCCAAAA AGGCTTCAGC CTTCAACAGC TGGTTTGAAA ATGCAGAGGA GGACTTAACA 
GACCCAGTGC GCTGCAACTC CCTGGAAGAG ATCAAGGCCT TGAGAGAGGC CCACGATGCC 
TTCCGCTCCT CGCTGAGCTC AGCCCAGGCC GACTTCAACC AGCTGGCTGA GCTGGACCGC 
CAGATCAAGA GCTTCCGCGT GGCCTCCAAC CCCTACACCT GGTTCACCAT GGAGGCCCTG 
GAGGAGACCT GGAGGAACCT TCAGAAGATC ATCAAGGAAA GGGAGCTGGA GCTGCAGAAG 
GAGCAGCGGC GGCAGGAGGA GAACGACAAG CTGCGCCAGG AGTTCGCCCA GCATGCCAAC 
GCCTTCCACC AGTGGATCCA GGAGACCAGA ACGTATCTCC TCGATGGCAT AGCGTATCGT 
CGGGTCATTC GTGTCTATCA GTATGAAGTT GGGGATGATC TGTCTGGAAG GTCCTGCATG 
GTGGAAGAAT CTGGGACCCT TGAATCGCAG CTTGAAGCTA CCAAACGCAA ACACCAGGAG 
ATTCGAGCCA TGAGAAGTCA GCTCAAGAAG ATTGAAGACC TGGGGGCGGC CATGGAGGAA 
GCGCTGATCC TGGACAACAA GTACACGGAG CACAGCACCG TGGGCCTGGC CCAGCAGTGG 
GACCAGCTCG ACCAGCTGGG CATGCGGATG CAGCACAACC TGGAGCAGCA GATCCAGGCC 
AGGAACACGA CAGGAGTGAC CGAGGAGGCC CTCAAAGAAT TCAGCATGAT GTTCAAACAC 
TTCGACAAGG ACAAGTCCGG CCGGCTGAAT CACCAGGAGT TCAAATCCTG TCTGCGCTCC 
CTGGGCTATG ACCTGCCCAT GGTGGAGGAA GGGGAGCCCG ACCCTGAGTT CGAGGCCATC 
TTGGATACTG TGGATCCTAA CAGGGACGGC CACGTCTCCC TGCAAGAGTA CATGGCCTTT 
ATGATCAGCC GTGAGACCGA GAATGTCAAG TCCAGCGAGG AGATTGAGAG CGCCTTCCGG 
GCCCTGAGCT CCGAAGGGAA GCCTTATGTG ACCAAGGAGG AGCTGTACCA GAACCTGACT 
CGGGAACAAG CCGACTACTG CGTCTCCCAC ATGAAGCCCT ACGTGGACGG CAAGGGCCGC 
GAACTCCCCA CTGCTTTCGA CTACGTTGAG TTCACCCGCT CGCTCTTCGT GAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTAN1 ( XM_012147482.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_012002872.1 Ovis aries musimon spectrin, alpha, non-erythrocytic 1 (SPTAN1), transcript variant X6, mRNA.

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CloneID	OOv24452
Clone ID Related Accession (Same CDS sequence)	XM_012147482.1
Accession Version	XM_012147482.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7485bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	spectrin alpha chain, non-erythrocytic 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942583.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTCAT CGTTTCGTAA ACGCAGAGTC CAGAAAATGG ACCCAAGCGG GGTCAAAGTG 
CTGGAGACAG CCGAGGACAT CCAGGAGAGG CGGCAGCAGG TCCTGGACAG ATACCACCGC 
TTCAAGGAGC TCTCGACCCT GAGGCGCCAG AAGCTTGAAG ACTCCTACCG ATTCCAGTTT 
TTCCAAAGAG ATGCTGAGGA GCTGGAGAAA TGGATTCAGG AGAAGCTTCA GATTGCCTCG 
GATGAGAATT ACAAAGACCC AACCAACCTG CAGGGCAAGC TGCAGAAGCA TCAAGCCTTC 
GAAGCCGAGG TGCAGGCCAA CTCGGGTGCC ATCGTGAAGC TTGATGAGAC CGGTAACCTG 
ATGATCTCAG AAGGGCACTT CGCGTCCGAA ACCATCCGGA CTCGTCTGAT GGAGCTGCAC 
CGCCAGTGGG AATTGCTTCT GGAGAAGATG CGGGAGAAAG GCATCAAGCT GCTGCAGGCC 
CAGAAGCTGG TGCAGTACCT GCGGGAGTGT GAGGACGTGA TGGACTGGAT CAATGACAAG 
GAAGCCATTG TGACATCTGA GGAGCTGGGC CAGGACCTGG AGCACGTGGA GGTGTTACAG 
AAGAAATTCG AAGAGTTTCA GACAGACATG GCTGCCCACG AAGAGAGAGT CAATGAAGTG 
AACCAGTTTG CTGCCAAGCT CATTCAGGAG CAGCACCCTG AGGAGGAGCT GATCAAGACA 
AAGGAGGACG AGGTGAATGC AGCCTGGCAG CGGCTGAAGG GCCTGGCCCT CCAGCGGCAG 
GGGAAGCTGT TCGGGGCAGC TGAGGTCCAA CGCTTTAACA GGGATGTGGA CGAGACCATT 
AGCTGGATTA AGGAGAAGGA ACAGTTAATG GCCTCTGATG ACTTTGGCCG GGACCTGGCC 
AGTGTCCAGG CTCTACTGCG GAAACACGAG GGTTTGGAGA GAGATCTTGC TGCCTTGGAG 
GACAAGGTCA AAGCCCTGTG TGCCGAGGCT GACCGCCTGC AGCAGTCCCA CCCTCTGAGT 
GCCACCCAGA TCCAAGTGAA GCGAGAGGAG CTGATCACCA ACTGGGAGCA GATCCGCACC 
CTGGCCGCGG AGAGACACGC ACGTCTCAAC GATTCCTACA GGCTTCAGCG CTTCCTTGCT 
GACTTCCGAG ACCTCACCAG CTGGGTCACT GAGATGAAAG CCCTCATCAA TGCAGATGAA 
CTTGCCAATG ATGTGGCCGG GGCCGAAGCC CTGCTGGATA GGCATCAGGA GCACAAGGGT 
GAAATTGACG CTCATGAAGA TAGCTTTAAA TCTGCAGATG AGTCTGGACA GGCCCTGCTT 
GCAGCTGGTC ACTATGCCTC AGATGAAGTG AGGGAGAAGC TGACCATCCT GTCCGAGGAG 
CGGGCCGCGC TACTAGAGCT CTGGGAGCTG CGACGGCAGC AATATGAGCA GTGCATGGAC 
CTGCAGCTCT TCTACCGCGA CACGGAGCAG GTGGACAACT GGATGAGCAA GCAGGAGGCG 
TTCCTGTTGA ACGAAGACCT GGGTGATTCC TTGGATAGTG TGGAAGCGCT TCTCAAGAAG 
CACGAAGACT TTGAGAAGTC CCTCAGCGCC CAGGAGGAGA AGATCACAGC CTTAGATGAG 
TTTGCCACCA AGCTGATTCA GAACAACCAC TACGCGATGG AAGATGTGGC CACCCGGCGA 
GACGCACTGC TGAGCCGCCG CAACGCCCTC CATGAGAGAG CCATGTACCG CCGTGCCCAG 
CTTGCAGATT CCTTCCACTT GCAGCAGTTT TTCCGTGACT CTGATGAGCT CAAGAGTTGG 
GTCAACGAGA AGATGAAAAC AGCCACAGAT GAAGCTTATA AAGACCCATC CAACCTACAA 
GGGAAAGTGC AGAAGCACCA GGCCTTTGAG GCCGAGCTCT CAGCAAACCA GAGCCGCATC 
GACGCCTTGG AGAAAGCTGG GCAGAAGCTG ATCGACGTTG ACCACTATGC CAAGGATGAG 
GTGGCGACTC GGATGAATGA AGTTATCAGT CTGTGGAAGA AGCTGCTGGA GGCCACGGAG 
CTGAAAGGCA TAAAGCTCCG TGAGGCTAAC CAGCAGCAGC AGTTTAACCG CAATGTTGAG 
GACATCGAGC TGTGGCTGTA CGAAGTGGAA GGTCATCTGG CCTCGGATGA CTACGGCAAA 
GACCTCACCA ACGTCCAGAA CCTCCAGAAA AAGCATGCCC TGCTGGAGGC TGACGTGGCC 
GCTCACCAGG ATCGCATCGA TGGCATCACT ATCCAAGCCC GCCAGTTCCA GGATGCGGGC 
CACTTTGATG CTGAAAACAT CAAGAAGAAG CAGGAGGCCC TGGTAGCTCG CTACGAGGCA 
CTCAAGGAGC CCATGGTTGC CCGGAAGCAG AAGCTGGCCG ATTCCCTGCG GTTGCAGCAG 
CTCTTCCGTG ACGTTGAGGA TGAGGAGACG TGGATTCGGG AGAAAGAACC TATTGCCGCC 
TCCACCAACA GAGGCAAAGA TTTAATTGGA GTCCAGAACC TGCTAAAGAA ACACCAAGCG 
CTACAAGCAG AAATTGCTGG CCACGAGCCC CGCATCAAAG CAGTCACACA GAAGGGGAAC 
GCCATGGTAG AGGAGGGCCA TTTTGCTGCC GAGGACGTGA AGGCCAAGCT CCATGAACTG 
AACCAGAAGT GGGAGTCGCT GAAGTCCAAG GCCTCCCAGC GACGGCAGGA CCTGGAGGAC 
TCGCTGCAGG CCCAGCAGTA CTTCGCTGAT GCCAACGAGG CCGAGTCCTG GATGCGGGAG 
AAGGAGCCCA TCGTGGGCAG CACCGACTAT GGCAAAGATG AGGACTCTGC CGAGGCTCTG 
CTAAAGAAGC ACGAGGCATT GATGTCGGAT CTCAGCGCCT ATGGCAGCAG CATCCAGGCC 
CTGCGAGAGC AGGCCCAGTC GTGCCGGCAA CAAGTGGCCC CCATGGATGA CGAGACTGGG 
AAGGAGCTGG TCTTGGCGCT CTATGACTAT CAGGAGAAGA GTCCCCGAGA GGTCACCATG 
AAGAAAGGAG ACATTCTCAC CTTACTCAAC AGCACCAACA AGGACTGGTG GAAAGTGGAA 
GTGAACGACC GGCAGGGCTT CGTGCCGGCC GCCTACGTGA AGAAGCTGGA CCCAGCGCAG 
TCGGCCTCCC GGGAGAACCT CCTGGAAGAG CAGGGCAGCA TCGCGCTGCG GCAGGAGCAG 
ATTGACAACC AGACGCGCGT AACTAAGGAG GCCGGCAGCG TGTCTCTGCG TATGAAACAG 
GTGGAAGAAC TGTACCATTC TCTGCTGGAA CTGGGAGAGA AGCGTAAAGG CATGTTGGAG 
AAGAGCTGTA AGAAGTTCAT GCTGTTCCGT GAAGCGAACG AGCTGCAGCA GTGGATCAAC 
GAGAAGGAAG CGGCCCTGAC AAGCGAGGAG GTCGGCGCGG ACTTGGAGCA GGTCGAAGTG 
CTGCAGAAGA AGTTTGACGA CTTCCAGAAG GATCTCAAGG CCAATGAGTC ACGGCTGAAG 
GACATTAACA AGGTGGCTGA AGACCTGGAG TCGGAAGGCC TGATGGCGGA GGAGGTGCAG 
GCCGTGCAGC AGCAGGAGGT GTATGGCGCG ATGCCCAGGG ATGAATCTGA TTCCAAGACA 
GCCTCCCCGT GGAAGGAGCT CAATGAGCGC TGGCGGTCCC TGCAGCAGCT GGCTGAGGAG 
CGGAGCCAAC TCTTGGGCAG CGCCCACGAA GTGCAGCGGT TCCACAGAGA TGCTGACGAA 
ACCAAAGAGT GGATCGAGGA GAAGAACCAG GCTCTAAACA CAGACAACTA TGGCCACGAC 
CTGGCCAGCG TCCAGGCCCT GCAGCGCAAG CATGAGGGCT TCGAGAGGGA CCTCGCGGCC 
CTTGGCGACA AGGTGAACTC GCTCGGGGAG ACAGCCGAGC GCCTGATCCA GTCCCACCCC 
GAGTCGGCAG AGGACCTGCA GGAGAAGTGC ACTGAGCTGA ACCAGGCATG GAGCAGCCTG 
GGCAAGCGTG CCGACCAGCG CAAGGCCAAG CTGGGCGACT CCCACGACCT GCAGCGCTTC 
CTCAGCGACT TCCGGGACCT CATGTCTTGG ATCAATGGAA TCCGAGGTCT GGTGTCCTCA 
GACGAGCTAG CCAAGGATGT CACTGGAGCT GAGGCCCTGC TGGAGCGACA CCAGGAACAC 
CGGACAGAAA TTGATGCCAG AGCTGGCACT TTCCAGGCAT TCGAGCAGTT TGGGCAGCAG 
CTGTTGGCTC ATGGGCACTA CGCCAGCCCC GAGATCAAGG AGAAGCTCGA TGTTCTAGAC 
CAGGAGCGCG CAGACCTCGA GAAGGCCTGG GTCCAGCGCA GGATGATGCT GGATCAGTGC 
CTTGAATTGC AGCTGTTCCA TCGGGATTGC GAGCAGGCCG AGAACTGGAT GGCTGCCCGG 
GAGGCCTTCC TGAACACAGA AGACAAAGGG GACTCGCTAG ACAGCGTGGA GGCTCTGATC 
AAAAAACACG AGGACTTTGA CAAAGCAATT AATGTCCAGG AGGAGAAGAT TGCTGCCCTG 
CAGTCCTTTG CTGACCAGCT CATTGCTGGC GGTCACTACG CCAAAGGCGA CATCTCCAGC 
CGGCGCAATG AGGTTCTGGA CAGGTGGCGC CGTCTAAAAG CTCAGATGAT TGAGAAGAGG 
TCAAAGCTGG GAGAATCGCA GACCCTCCAG CAGTTCAGCC GGGATGTAGA CGAGATTGAA 
GCTTGGATCA GTGAAAAATT ACAGACTGCA AGTGATGAGT CATATAAGGA CCCCACCAAC 
ATCCAGCTTT CCAAGCTGCT GAGCAAGCAT CAGAAGCACC AGGCCTTCGA GGCAGAACTG 
CACGCCAATG CGGACCGGAT CCGCGGGGTC ATCGACGTGG GCAACTCCCT CATTGACCGT 
GGGGCCTGTG CCGGCAGCGA GGATGCCGTC AAGGCCCGGC TGGCCGCCTT AGCTGACCAG 
TGGCAGTTCC TGGTGCAGAA GTCGGCTGAG AAGAGCCAGA AGCTGAAAGA AGCCAACAAA 
CAGCAGAACT TCAACACTGG CATCAAGGAC TTCGACTTCT GGCTCTCTGA GGTGGAAGCC 
CTGCTGGCCT CTGAGGATTA TGGCAAAGAC CTGGCTTCTG TGAACAACTT GCTAAAAAAA 
CATCAGCTGC TGGAGGCAGA TATCTCTGCC CACGAGGACC GCCTGAAGGA CCTCAACAGC 
CAGGCGGACA GCCTGATGAC CAGCAGCGCC TTCGACACCT CCCAGGTGAA GGATAAGCGG 
GACACCATCA ACAGCCGTTT CCAGAAGATC AAGAGCATGG CCGCCTCCCG GCGAGCTAGG 
CTGAATGAGT CCCACCGCCT GCACCAGTTC TTCCGGGACA TGGACGATGA GGAGTCCTGG 
ATCAAGGAGA AGAAGCTGCT GGTGAGCTCA GAGGACTACG GCCGCGACCT GACGGGCGTC 
CAGAACCTGA GGAAGAAGCA CAAACGGCTG GAGGCAGAGC TGGCTGCCCA CGAGCCGGCC 
ATTCAGGGTG TCCTGGACAC TGGAAAGAAG CTGTCTGATG ACAACACCAT CGGGAAGGAG 
GAGATTCAGC AGCGCCTGGC GCAGTTTGTG GAGCACTGGA AGGAGCTGAA GCAGCTGGCA 
GCTGCTCGGG GCCAGCGGCT AGAAGAATCT TTGGAATATC AGCAGTTTGT GGCCAACGTG 
GAGGAGGAGG AAGCCTGGAT CAATGAGAAG ATGACCCTGG TGGCCAGTGA GGATTACGGA 
GACACTCTTG CTGCCATCCA GGGCTTGCTG AAGAAACACG AAGCCTTCGA GACGGACTTC 
ACTGTCCACA AGGACCGGGT GAATGACGTC TGCACTAACG GACAAGACCT GGTTAAGAAG 
AACAATCACC ATGAGGAGAA CATCTCTTCC AAGATGAAGG GCTTGAACGG GAAAGTGTCA 
GACTTGGAGA AGGCTGCCTC CCAGAGGAAG GCAAAGCTCG ACGAGAACTC GGCCTTCCTC 
CAGTTCAACT GGAAGGCCGA TGTGGTGGAG TCCTGGATTG GTGAAAAGGA GAACAGCTTG 
AAGACAGATG ATTATGGTCG AGATCTGTCT TCTGTCCAAA CTCTCCTCAC CAAACAGGAA 
ACCTTCGATG CTGGCCTGCA GGCCTTCCAG CAGGAGGGCA TCGCCAACAT CACTGCCCTC 
AAAGATCAGC TGCTGGCCGC CAAGCATGTT CAGTCCAAGG CCATCGAGGC CCGCCACGCT 
GCCCTCATGA AGAGATGGAG CCAGCTCCTG GCCAATTCGG CCACCCGGAA GAAGAAGCTG 
CTGGAGGCAC AGAGTCACTT CCGCAAGGTG GAGGATCTCT TCCTGACCTT TGCCAAAAAG 
GCTTCAGCCT TCAACAGCTG GTTTGAAAAT GCAGAGGAGG ACTTAACAGA CCCAGTGCGC 
TGCAACTCCC TGGAAGAGAT CAAGGCCTTG AGAGAGGCCC ACGATGCCTT CCGCTCCTCG 
CTGAGCTCAG CCCAGGCCGA CTTCAACCAG CTGGCTGAGC TGGACCGCCA GATCAAGAGC 
TTCCGCGTGG CCTCCAACCC CTACACCTGG TTCACCATGG AGGCCCTGGA GGAGACCTGG 
AGGAACCTTC AGAAGATCAT CAAGGAAAGG GAGCTGGAGC TGCAGAAGGA GCAGCGGCGG 
CAGGAGGAGA ACGACAAGCT GCGCCAGGAG TTCGCCCAGC ATGCCAACGC CTTCCACCAG 
TGGATCCAGG AGACCAGAAC GTATCTCCTC GATGGCATAG CGTATCGTCG GGTCATTCGT 
GTCTATCAGT ATGAAGTTGG GGATGATCTG TCTGGAAGGT CCTGCATGGT GGAAGAATCT 
GGGACCCTTG AATCGCAGCT TGAAGCTACC AAACGCAAAC ACCAGGAGAT TCGAGCCATG 
AGAAGTCAGC TCAAGAAGAT TGAAGACCTG GGGGCGGCCA TGGAGGAAGC GCTGATCCTG 
GACAACAAGT ACACGGAGCA CAGCACCGTG GGCCTGGCCC AGCAGTGGGA CCAGCTCGAC 
CAGCTGGGCA TGCGGATGCA GCACAACCTG GAGCAGCAGA TCCAGGCCAG GAACACGACA 
GGAGTGACCG AGGAGGCCCT CAAAGAATTC AGCATGATGT TCAAACACTT CGACAAGGAC 
AAGTCCGGCC GGCTGAATCA CCAGGAGTTC AAATCCTGTC TGCGCTCCCT GGGCTATGAC 
CTGCCCATGG TGGAGGAAGG GGAGCCCGAC CCTGAGTTCG AGGCCATCTT GGATACTGTG 
GATCCTAACA GGGACGGCCA CGTCTCCCTG CAAGAGTACA TGGCCTTTAT GATCAGCCGT 
GAGACCGAGA ATGTCAAGTC CAGCGAGGAG ATTGAGAGCG CCTTCCGGGC CCTGAGCTCC 
GAAGGGAAGC CTTATGTGAC CAAGGAGGAG CTGTACCAGA ACCTGACTCG GGAACAAGCC 
GACTACTGCG TCTCCCACAT GAAGCCCTAC GTGGACGGCA AGGGCCGCGA ACTCCCCACT 
GCTTTCGACT ACGTTGAGTT CACCCGCTCG CTCTTCGTGA ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012002872.1
CDS	82..7566
Translation	MLSSFRKRRVQKMDPSGVKVLETAEDIQERRQQVLDRYHRFKELSTLRRQKLEDSYRFQFFQRDAEELEKWIQEKLQIASDENYKDPTNLQGKLQKHQAFEAEVQANSGAIVKLDETGNLMISEGHFASETIRTRLMELHRQWELLLEKMREKGIKLLQAQKLVQYLRECEDVMDWINDKEAIVTSEELGQDLEHVEVLQKKFEEFQTDMAAHEERVNEVNQFAAKLIQEQHPEEELIKTKEDEVNAAWQRLKGLALQRQGKLFGAAEVQRFNRDVDETISWIKEKEQLMASDDFGRDLASVQALLRKHEGLERDLAALEDKVKALCAEADRLQQSHPLSATQIQVKREELITNWEQIRTLAAERHARLNDSYRLQRFLADFRDLTSWVTEMKALINADELANDVAGAEALLDRHQEHKGEIDAHEDSFKSADESGQALLAAGHYASDEVREKLTILSEERAALLELWELRRQQYEQCMDLQLFYRDTEQVDNWMSKQEAFLLNEDLGDSLDSVEALLKKHEDFEKSLSAQEEKITALDEFATKLIQNNHYAMEDVATRRDALLSRRNALHERAMYRRAQLADSFHLQQFFRDSDELKSWVNEKMKTATDEAYKDPSNLQGKVQKHQAFEAELSANQSRIDALEKAGQKLIDVDHYAKDEVATRMNEVISLWKKLLEATELKGIKLREANQQQQFNRNVEDIELWLYEVEGHLASDDYGKDLTNVQNLQKKHALLEADVAAHQDRIDGITIQARQFQDAGHFDAENIKKKQEALVARYEALKEPMVARKQKLADSLRLQQLFRDVEDEETWIREKEPIAASTNRGKDLIGVQNLLKKHQALQAEIAGHEPRIKAVTQKGNAMVEEGHFAAEDVKAKLHELNQKWESLKSKASQRRQDLEDSLQAQQYFADANEAESWMREKEPIVGSTDYGKDEDSAEALLKKHEALMSDLSAYGSSIQALREQAQSCRQQVAPMDDETGKELVLALYDYQEKSPREVTMKKGDILTLLNSTNKDWWKVEVNDRQGFVPAAYVKKLDPAQSASRENLLEEQGSIALRQEQIDNQTRVTKEAGSVSLRMKQVEELYHSLLELGEKRKGMLEKSCKKFMLFREANELQQWINEKEAALTSEEVGADLEQVEVLQKKFDDFQKDLKANESRLKDINKVAEDLESEGLMAEEVQAVQQQEVYGAMPRDESDSKTASPWKELNERWRSLQQLAEERSQLLGSAHEVQRFHRDADETKEWIEEKNQALNTDNYGHDLASVQALQRKHEGFERDLAALGDKVNSLGETAERLIQSHPESAEDLQEKCTELNQAWSSLGKRADQRKAKLGDSHDLQRFLSDFRDLMSWINGIRGLVSSDELAKDVTGAEALLERHQEHRTEIDARAGTFQAFEQFGQQLLAHGHYASPEIKEKLDVLDQERADLEKAWVQRRMMLDQCLELQLFHRDCEQAENWMAAREAFLNTEDKGDSLDSVEALIKKHEDFDKAINVQEEKIAALQSFADQLIAGGHYAKGDISSRRNEVLDRWRRLKAQMIEKRSKLGESQTLQQFSRDVDEIEAWISEKLQTASDESYKDPTNIQLSKLLSKHQKHQAFEAELHANADRIRGVIDVGNSLIDRGACAGSEDAVKARLAALADQWQFLVQKSAEKSQKLKEANKQQNFNTGIKDFDFWLSEVEALLASEDYGKDLASVNNLLKKHQLLEADISAHEDRLKDLNSQADSLMTSSAFDTSQVKDKRDTINSRFQKIKSMAASRRARLNESHRLHQFFRDMDDEESWIKEKKLLVSSEDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQFVEHWKELKQLAAARGQRLEESLEYQQFVANVEEEEAWINEKMTLVASEDYGDTLAAIQGLLKKHEAFETDFTVHKDRVNDVCTNGQDLVKKNNHHEENISSKMKGLNGKVSDLEKAASQRKAKLDENSAFLQFNWKADVVESWIGEKENSLKTDDYGRDLSSVQTLLTKQETFDAGLQAFQQEGIANITALKDQLLAAKHVQSKAIEARHAALMKRWSQLLANSATRKKKLLEAQSHFRKVEDLFLTFAKKASAFNSWFENAEEDLTDPVRCNSLEEIKALREAHDAFRSSLSSAQADFNQLAELDRQIKSFRVASNPYTWFTMEALEETWRNLQKIIKERELELQKEQRRQEENDKLRQEFAQHANAFHQWIQETRTYLLDGIAYRRVIRVYQYEVGDDLSGRSCMVEESGTLESQLEATKRKHQEIRAMRSQLKKIEDLGAAMEEALILDNKYTEHSTVGLAQQWDQLDQLGMRMQHNLEQQIQARNTTGVTEEALKEFSMMFKHFDKDKSGRLNHQEFKSCLRSLGYDLPMVEEGEPDPEFEAILDTVDPNRDGHVSLQEYMAFMISRETENVKSSEEIESAFRALSSEGKPYVTKEELYQNLTREQADYCVSHMKPYVDGKGRELPTAFDYVEFTRSLFVN

Target ORF information:

RefSeq Version	XM_012147482.1
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon spectrin, alpha, non-erythrocytic 1 (SPTAN1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012147482.1

ORF Insert Sequence:

ATGCTGTCAT CGTTTCGTAA ACGCAGAGTC CAGAAAATGG ACCCAAGCGG GGTCAAAGTG 
CTGGAGACAG CCGAGGACAT CCAGGAGAGG CGGCAGCAGG TCCTGGACAG ATACCACCGC 
TTCAAGGAGC TCTCGACCCT GAGGCGCCAG AAGCTTGAAG ACTCCTACCG ATTCCAGTTT 
TTCCAAAGAG ATGCTGAGGA GCTGGAGAAA TGGATTCAGG AGAAGCTTCA GATTGCCTCG 
GATGAGAATT ACAAAGACCC AACCAACCTG CAGGGCAAGC TGCAGAAGCA TCAAGCCTTC 
GAAGCCGAGG TGCAGGCCAA CTCGGGTGCC ATCGTGAAGC TTGATGAGAC CGGTAACCTG 
ATGATCTCAG AAGGGCACTT CGCGTCCGAA ACCATCCGGA CTCGTCTGAT GGAGCTGCAC 
CGCCAGTGGG AATTGCTTCT GGAGAAGATG CGGGAGAAAG GCATCAAGCT GCTGCAGGCC 
CAGAAGCTGG TGCAGTACCT GCGGGAGTGT GAGGACGTGA TGGACTGGAT CAATGACAAG 
GAAGCCATTG TGACATCTGA GGAGCTGGGC CAGGACCTGG AGCACGTGGA GGTGTTACAG 
AAGAAATTCG AAGAGTTTCA GACAGACATG GCTGCCCACG AAGAGAGAGT CAATGAAGTG 
AACCAGTTTG CTGCCAAGCT CATTCAGGAG CAGCACCCTG AGGAGGAGCT GATCAAGACA 
AAGGAGGACG AGGTGAATGC AGCCTGGCAG CGGCTGAAGG GCCTGGCCCT CCAGCGGCAG 
GGGAAGCTGT TCGGGGCAGC TGAGGTCCAA CGCTTTAACA GGGATGTGGA CGAGACCATT 
AGCTGGATTA AGGAGAAGGA ACAGTTAATG GCCTCTGATG ACTTTGGCCG GGACCTGGCC 
AGTGTCCAGG CTCTACTGCG GAAACACGAG GGTTTGGAGA GAGATCTTGC TGCCTTGGAG 
GACAAGGTCA AAGCCCTGTG TGCCGAGGCT GACCGCCTGC AGCAGTCCCA CCCTCTGAGT 
GCCACCCAGA TCCAAGTGAA GCGAGAGGAG CTGATCACCA ACTGGGAGCA GATCCGCACC 
CTGGCCGCGG AGAGACACGC ACGTCTCAAC GATTCCTACA GGCTTCAGCG CTTCCTTGCT 
GACTTCCGAG ACCTCACCAG CTGGGTCACT GAGATGAAAG CCCTCATCAA TGCAGATGAA 
CTTGCCAATG ATGTGGCCGG GGCCGAAGCC CTGCTGGATA GGCATCAGGA GCACAAGGGT 
GAAATTGACG CTCATGAAGA TAGCTTTAAA TCTGCAGATG AGTCTGGACA GGCCCTGCTT 
GCAGCTGGTC ACTATGCCTC AGATGAAGTG AGGGAGAAGC TGACCATCCT GTCCGAGGAG 
CGGGCCGCGC TACTAGAGCT CTGGGAGCTG CGACGGCAGC AATATGAGCA GTGCATGGAC 
CTGCAGCTCT TCTACCGCGA CACGGAGCAG GTGGACAACT GGATGAGCAA GCAGGAGGCG 
TTCCTGTTGA ACGAAGACCT GGGTGATTCC TTGGATAGTG TGGAAGCGCT TCTCAAGAAG 
CACGAAGACT TTGAGAAGTC CCTCAGCGCC CAGGAGGAGA AGATCACAGC CTTAGATGAG 
TTTGCCACCA AGCTGATTCA GAACAACCAC TACGCGATGG AAGATGTGGC CACCCGGCGA 
GACGCACTGC TGAGCCGCCG CAACGCCCTC CATGAGAGAG CCATGTACCG CCGTGCCCAG 
CTTGCAGATT CCTTCCACTT GCAGCAGTTT TTCCGTGACT CTGATGAGCT CAAGAGTTGG 
GTCAACGAGA AGATGAAAAC AGCCACAGAT GAAGCTTATA AAGACCCATC CAACCTACAA 
GGGAAAGTGC AGAAGCACCA GGCCTTTGAG GCCGAGCTCT CAGCAAACCA GAGCCGCATC 
GACGCCTTGG AGAAAGCTGG GCAGAAGCTG ATCGACGTTG ACCACTATGC CAAGGATGAG 
GTGGCGACTC GGATGAATGA AGTTATCAGT CTGTGGAAGA AGCTGCTGGA GGCCACGGAG 
CTGAAAGGCA TAAAGCTCCG TGAGGCTAAC CAGCAGCAGC AGTTTAACCG CAATGTTGAG 
GACATCGAGC TGTGGCTGTA CGAAGTGGAA GGTCATCTGG CCTCGGATGA CTACGGCAAA 
GACCTCACCA ACGTCCAGAA CCTCCAGAAA AAGCATGCCC TGCTGGAGGC TGACGTGGCC 
GCTCACCAGG ATCGCATCGA TGGCATCACT ATCCAAGCCC GCCAGTTCCA GGATGCGGGC 
CACTTTGATG CTGAAAACAT CAAGAAGAAG CAGGAGGCCC TGGTAGCTCG CTACGAGGCA 
CTCAAGGAGC CCATGGTTGC CCGGAAGCAG AAGCTGGCCG ATTCCCTGCG GTTGCAGCAG 
CTCTTCCGTG ACGTTGAGGA TGAGGAGACG TGGATTCGGG AGAAAGAACC TATTGCCGCC 
TCCACCAACA GAGGCAAAGA TTTAATTGGA GTCCAGAACC TGCTAAAGAA ACACCAAGCG 
CTACAAGCAG AAATTGCTGG CCACGAGCCC CGCATCAAAG CAGTCACACA GAAGGGGAAC 
GCCATGGTAG AGGAGGGCCA TTTTGCTGCC GAGGACGTGA AGGCCAAGCT CCATGAACTG 
AACCAGAAGT GGGAGTCGCT GAAGTCCAAG GCCTCCCAGC GACGGCAGGA CCTGGAGGAC 
TCGCTGCAGG CCCAGCAGTA CTTCGCTGAT GCCAACGAGG CCGAGTCCTG GATGCGGGAG 
AAGGAGCCCA TCGTGGGCAG CACCGACTAT GGCAAAGATG AGGACTCTGC CGAGGCTCTG 
CTAAAGAAGC ACGAGGCATT GATGTCGGAT CTCAGCGCCT ATGGCAGCAG CATCCAGGCC 
CTGCGAGAGC AGGCCCAGTC GTGCCGGCAA CAAGTGGCCC CCATGGATGA CGAGACTGGG 
AAGGAGCTGG TCTTGGCGCT CTATGACTAT CAGGAGAAGA GTCCCCGAGA GGTCACCATG 
AAGAAAGGAG ACATTCTCAC CTTACTCAAC AGCACCAACA AGGACTGGTG GAAAGTGGAA 
GTGAACGACC GGCAGGGCTT CGTGCCGGCC GCCTACGTGA AGAAGCTGGA CCCAGCGCAG 
TCGGCCTCCC GGGAGAACCT CCTGGAAGAG CAGGGCAGCA TCGCGCTGCG GCAGGAGCAG 
ATTGACAACC AGACGCGCGT AACTAAGGAG GCCGGCAGCG TGTCTCTGCG TATGAAACAG 
GTGGAAGAAC TGTACCATTC TCTGCTGGAA CTGGGAGAGA AGCGTAAAGG CATGTTGGAG 
AAGAGCTGTA AGAAGTTCAT GCTGTTCCGT GAAGCGAACG AGCTGCAGCA GTGGATCAAC 
GAGAAGGAAG CGGCCCTGAC AAGCGAGGAG GTCGGCGCGG ACTTGGAGCA GGTCGAAGTG 
CTGCAGAAGA AGTTTGACGA CTTCCAGAAG GATCTCAAGG CCAATGAGTC ACGGCTGAAG 
GACATTAACA AGGTGGCTGA AGACCTGGAG TCGGAAGGCC TGATGGCGGA GGAGGTGCAG 
GCCGTGCAGC AGCAGGAGGT GTATGGCGCG ATGCCCAGGG ATGAATCTGA TTCCAAGACA 
GCCTCCCCGT GGAAGGAGCT CAATGAGCGC TGGCGGTCCC TGCAGCAGCT GGCTGAGGAG 
CGGAGCCAAC TCTTGGGCAG CGCCCACGAA GTGCAGCGGT TCCACAGAGA TGCTGACGAA 
ACCAAAGAGT GGATCGAGGA GAAGAACCAG GCTCTAAACA CAGACAACTA TGGCCACGAC 
CTGGCCAGCG TCCAGGCCCT GCAGCGCAAG CATGAGGGCT TCGAGAGGGA CCTCGCGGCC 
CTTGGCGACA AGGTGAACTC GCTCGGGGAG ACAGCCGAGC GCCTGATCCA GTCCCACCCC 
GAGTCGGCAG AGGACCTGCA GGAGAAGTGC ACTGAGCTGA ACCAGGCATG GAGCAGCCTG 
GGCAAGCGTG CCGACCAGCG CAAGGCCAAG CTGGGCGACT CCCACGACCT GCAGCGCTTC 
CTCAGCGACT TCCGGGACCT CATGTCTTGG ATCAATGGAA TCCGAGGTCT GGTGTCCTCA 
GACGAGCTAG CCAAGGATGT CACTGGAGCT GAGGCCCTGC TGGAGCGACA CCAGGAACAC 
CGGACAGAAA TTGATGCCAG AGCTGGCACT TTCCAGGCAT TCGAGCAGTT TGGGCAGCAG 
CTGTTGGCTC ATGGGCACTA CGCCAGCCCC GAGATCAAGG AGAAGCTCGA TGTTCTAGAC 
CAGGAGCGCG CAGACCTCGA GAAGGCCTGG GTCCAGCGCA GGATGATGCT GGATCAGTGC 
CTTGAATTGC AGCTGTTCCA TCGGGATTGC GAGCAGGCCG AGAACTGGAT GGCTGCCCGG 
GAGGCCTTCC TGAACACAGA AGACAAAGGG GACTCGCTAG ACAGCGTGGA GGCTCTGATC 
AAAAAACACG AGGACTTTGA CAAAGCAATT AATGTCCAGG AGGAGAAGAT TGCTGCCCTG 
CAGTCCTTTG CTGACCAGCT CATTGCTGGC GGTCACTACG CCAAAGGCGA CATCTCCAGC 
CGGCGCAATG AGGTTCTGGA CAGGTGGCGC CGTCTAAAAG CTCAGATGAT TGAGAAGAGG 
TCAAAGCTGG GAGAATCGCA GACCCTCCAG CAGTTCAGCC GGGATGTAGA CGAGATTGAA 
GCTTGGATCA GTGAAAAATT ACAGACTGCA AGTGATGAGT CATATAAGGA CCCCACCAAC 
ATCCAGCTTT CCAAGCTGCT GAGCAAGCAT CAGAAGCACC AGGCCTTCGA GGCAGAACTG 
CACGCCAATG CGGACCGGAT CCGCGGGGTC ATCGACGTGG GCAACTCCCT CATTGACCGT 
GGGGCCTGTG CCGGCAGCGA GGATGCCGTC AAGGCCCGGC TGGCCGCCTT AGCTGACCAG 
TGGCAGTTCC TGGTGCAGAA GTCGGCTGAG AAGAGCCAGA AGCTGAAAGA AGCCAACAAA 
CAGCAGAACT TCAACACTGG CATCAAGGAC TTCGACTTCT GGCTCTCTGA GGTGGAAGCC 
CTGCTGGCCT CTGAGGATTA TGGCAAAGAC CTGGCTTCTG TGAACAACTT GCTAAAAAAA 
CATCAGCTGC TGGAGGCAGA TATCTCTGCC CACGAGGACC GCCTGAAGGA CCTCAACAGC 
CAGGCGGACA GCCTGATGAC CAGCAGCGCC TTCGACACCT CCCAGGTGAA GGATAAGCGG 
GACACCATCA ACAGCCGTTT CCAGAAGATC AAGAGCATGG CCGCCTCCCG GCGAGCTAGG 
CTGAATGAGT CCCACCGCCT GCACCAGTTC TTCCGGGACA TGGACGATGA GGAGTCCTGG 
ATCAAGGAGA AGAAGCTGCT GGTGAGCTCA GAGGACTACG GCCGCGACCT GACGGGCGTC 
CAGAACCTGA GGAAGAAGCA CAAACGGCTG GAGGCAGAGC TGGCTGCCCA CGAGCCGGCC 
ATTCAGGGTG TCCTGGACAC TGGAAAGAAG CTGTCTGATG ACAACACCAT CGGGAAGGAG 
GAGATTCAGC AGCGCCTGGC GCAGTTTGTG GAGCACTGGA AGGAGCTGAA GCAGCTGGCA 
GCTGCTCGGG GCCAGCGGCT AGAAGAATCT TTGGAATATC AGCAGTTTGT GGCCAACGTG 
GAGGAGGAGG AAGCCTGGAT CAATGAGAAG ATGACCCTGG TGGCCAGTGA GGATTACGGA 
GACACTCTTG CTGCCATCCA GGGCTTGCTG AAGAAACACG AAGCCTTCGA GACGGACTTC 
ACTGTCCACA AGGACCGGGT GAATGACGTC TGCACTAACG GACAAGACCT GGTTAAGAAG 
AACAATCACC ATGAGGAGAA CATCTCTTCC AAGATGAAGG GCTTGAACGG GAAAGTGTCA 
GACTTGGAGA AGGCTGCCTC CCAGAGGAAG GCAAAGCTCG ACGAGAACTC GGCCTTCCTC 
CAGTTCAACT GGAAGGCCGA TGTGGTGGAG TCCTGGATTG GTGAAAAGGA GAACAGCTTG 
AAGACAGATG ATTATGGTCG AGATCTGTCT TCTGTCCAAA CTCTCCTCAC CAAACAGGAA 
ACCTTCGATG CTGGCCTGCA GGCCTTCCAG CAGGAGGGCA TCGCCAACAT CACTGCCCTC 
AAAGATCAGC TGCTGGCCGC CAAGCATGTT CAGTCCAAGG CCATCGAGGC CCGCCACGCT 
GCCCTCATGA AGAGATGGAG CCAGCTCCTG GCCAATTCGG CCACCCGGAA GAAGAAGCTG 
CTGGAGGCAC AGAGTCACTT CCGCAAGGTG GAGGATCTCT TCCTGACCTT TGCCAAAAAG 
GCTTCAGCCT TCAACAGCTG GTTTGAAAAT GCAGAGGAGG ACTTAACAGA CCCAGTGCGC 
TGCAACTCCC TGGAAGAGAT CAAGGCCTTG AGAGAGGCCC ACGATGCCTT CCGCTCCTCG 
CTGAGCTCAG CCCAGGCCGA CTTCAACCAG CTGGCTGAGC TGGACCGCCA GATCAAGAGC 
TTCCGCGTGG CCTCCAACCC CTACACCTGG TTCACCATGG AGGCCCTGGA GGAGACCTGG 
AGGAACCTTC AGAAGATCAT CAAGGAAAGG GAGCTGGAGC TGCAGAAGGA GCAGCGGCGG 
CAGGAGGAGA ACGACAAGCT GCGCCAGGAG TTCGCCCAGC ATGCCAACGC CTTCCACCAG 
TGGATCCAGG AGACCAGAAC GTATCTCCTC GATGGCATAG CGTATCGTCG GGTCATTCGT 
GTCTATCAGT ATGAAGTTGG GGATGATCTG TCTGGAAGGT CCTGCATGGT GGAAGAATCT 
GGGACCCTTG AATCGCAGCT TGAAGCTACC AAACGCAAAC ACCAGGAGAT TCGAGCCATG 
AGAAGTCAGC TCAAGAAGAT TGAAGACCTG GGGGCGGCCA TGGAGGAAGC GCTGATCCTG 
GACAACAAGT ACACGGAGCA CAGCACCGTG GGCCTGGCCC AGCAGTGGGA CCAGCTCGAC 
CAGCTGGGCA TGCGGATGCA GCACAACCTG GAGCAGCAGA TCCAGGCCAG GAACACGACA 
GGAGTGACCG AGGAGGCCCT CAAAGAATTC AGCATGATGT TCAAACACTT CGACAAGGAC 
AAGTCCGGCC GGCTGAATCA CCAGGAGTTC AAATCCTGTC TGCGCTCCCT GGGCTATGAC 
CTGCCCATGG TGGAGGAAGG GGAGCCCGAC CCTGAGTTCG AGGCCATCTT GGATACTGTG 
GATCCTAACA GGGACGGCCA CGTCTCCCTG CAAGAGTACA TGGCCTTTAT GATCAGCCGT 
GAGACCGAGA ATGTCAAGTC CAGCGAGGAG ATTGAGAGCG CCTTCCGGGC CCTGAGCTCC 
GAAGGGAAGC CTTATGTGAC CAAGGAGGAG CTGTACCAGA ACCTGACTCG GGAACAAGCC 
GACTACTGCG TCTCCCACAT GAAGCCCTAC GTGGACGGCA AGGGCCGCGA ACTCCCCACT 
GCTTTCGACT ACGTTGAGTT CACCCGCTCG CTCTTCGTGA ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTAN1 ( XM_012147483.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_012002873.1 Ovis aries musimon spectrin, alpha, non-erythrocytic 1 (SPTAN1), transcript variant X7, mRNA.

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CloneID	OOv24453
Clone ID Related Accession (Same CDS sequence)	XM_012147483.1
Accession Version	XM_012147483.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7473bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	spectrin alpha chain, non-erythrocytic 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942583.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTCAT CGTTTCGTAA ACGCAGAGTC CAGAAAATGG ACCCAAGCGG GGTCAAAGTG 
CTGGAGACAG CCGAGGACAT CCAGGAGAGG CGGCAGCAGG TCCTGGACAG ATACCACCGC 
TTCAAGGAGC TCTCGACCCT GAGGCGCCAG AAGCTTGAAG ACTCCTACCG ATTCCAGTTT 
TTCCAAAGAG ATGCTGAGGA GCTGGAGAAA TGGATTCAGG AGAAGCTTCA GATTGCCTCG 
GATGAGAATT ACAAAGACCC AACCAACCTG CAGGGCAAGC TGCAGAAGCA TCAAGCCTTC 
GAAGCCGAGG TGCAGGCCAA CTCGGGTGCC ATCGTGAAGC TTGATGAGAC CGGTAACCTG 
ATGATCTCAG AAGGGCACTT CGCGTCCGAA ACCATCCGGA CTCGTCTGAT GGAGCTGCAC 
CGCCAGTGGG AATTGCTTCT GGAGAAGATG CGGGAGAAAG GCATCAAGCT GCTGCAGGCC 
CAGAAGCTGG TGCAGTACCT GCGGGAGTGT GAGGACGTGA TGGACTGGAT CAATGACAAG 
GAAGCCATTG TGACATCTGA GGAGCTGGGC CAGGACCTGG AGCACGTGGA GGTGTTACAG 
AAGAAATTCG AAGAGTTTCA GACAGACATG GCTGCCCACG AAGAGAGAGT CAATGAAGTG 
AACCAGTTTG CTGCCAAGCT CATTCAGGAG CAGCACCCTG AGGAGGAGCT GATCAAGACA 
AAGGAGGACG AGGTGAATGC AGCCTGGCAG CGGCTGAAGG GCCTGGCCCT CCAGCGGCAG 
GGGAAGCTGT TCGGGGCAGC TGAGGTCCAA CGCTTTAACA GGGATGTGGA CGAGACCATT 
AGCTGGATTA AGGAGAAGGA ACAGTTAATG GCCTCTGATG ACTTTGGCCG GGACCTGGCC 
AGTGTCCAGG CTCTACTGCG GAAACACGAG GGTTTGGAGA GAGATCTTGC TGCCTTGGAG 
GACAAGGTCA AAGCCCTGTG TGCCGAGGCT GACCGCCTGC AGCAGTCCCA CCCTCTGAGT 
GCCACCCAGA TCCAAGTGAA GCGAGAGGAG CTGATCACCA ACTGGGAGCA GATCCGCACC 
CTGGCCGCGG AGAGACACGC ACGTCTCAAC GATTCCTACA GGCTTCAGCG CTTCCTTGCT 
GACTTCCGAG ACCTCACCAG CTGGGTCACT GAGATGAAAG CCCTCATCAA TGCAGATGAA 
CTTGCCAATG ATGTGGCCGG GGCCGAAGCC CTGCTGGATA GGCATCAGGA GCACAAGGGT 
GAAATTGACG CTCATGAAGA TAGCTTTAAA TCTGCAGATG AGTCTGGACA GGCCCTGCTT 
GCAGCTGGTC ACTATGCCTC AGATGAAGTG AGGGAGAAGC TGACCATCCT GTCCGAGGAG 
CGGGCCGCGC TACTAGAGCT CTGGGAGCTG CGACGGCAGC AATATGAGCA GTGCATGGAC 
CTGCAGCTCT TCTACCGCGA CACGGAGCAG GTGGACAACT GGATGAGCAA GCAGGAGGCG 
TTCCTGTTGA ACGAAGACCT GGGTGATTCC TTGGATAGTG TGGAAGCGCT TCTCAAGAAG 
CACGAAGACT TTGAGAAGTC CCTCAGCGCC CAGGAGGAGA AGATCACAGC CTTAGATGAG 
TTTGCCACCA AGCTGATTCA GAACAACCAC TACGCGATGG AAGATGTGGC CACCCGGCGA 
GACGCACTGC TGAGCCGCCG CAACGCCCTC CATGAGAGAG CCATGTACCG CCGTGCCCAG 
CTTGCAGATT CCTTCCACTT GCAGCAGTTT TTCCGTGACT CTGATGAGCT CAAGAGTTGG 
GTCAACGAGA AGATGAAAAC AGCCACAGAT GAAGCTTATA AAGACCCATC CAACCTACAA 
GGGAAAGTGC AGAAGCACCA GGCCTTTGAG GCCGAGCTCT CAGCAAACCA GAGCCGCATC 
GACGCCTTGG AGAAAGCTGG GCAGAAGCTG ATCGACGTTG ACCACTATGC CAAGGATGAG 
GTGGCGACTC GGATGAATGA AGTTATCAGT CTGTGGAAGA AGCTGCTGGA GGCCACGGAG 
CTGAAAGGCA TAAAGCTCCG TGAGGCTAAC CAGCAGCAGC AGTTTAACCG CAATGTTGAG 
GACATCGAGC TGTGGCTGTA CGAAGTGGAA GGTCATCTGG CCTCGGATGA CTACGGCAAA 
GACCTCACCA ACGTCCAGAA CCTCCAGAAA AAGCATGCCC TGCTGGAGGC TGACGTGGCC 
GCTCACCAGG ATCGCATCGA TGGCATCACT ATCCAAGCCC GCCAGTTCCA GGATGCGGGC 
CACTTTGATG CTGAAAACAT CAAGAAGAAG CAGGAGGCCC TGGTAGCTCG CTACGAGGCA 
CTCAAGGAGC CCATGGTTGC CCGGAAGCAG AAGCTGGCCG ATTCCCTGCG GTTGCAGCAG 
CTCTTCCGTG ACGTTGAGGA TGAGGAGACG TGGATTCGGG AGAAAGAACC TATTGCCGCC 
TCCACCAACA GAGGCAAAGA TTTAATTGGA GTCCAGAACC TGCTAAAGAA ACACCAAGCG 
CTACAAGCAG AAATTGCTGG CCACGAGCCC CGCATCAAAG CAGTCACACA GAAGGGGAAC 
GCCATGGTAG AGGAGGGCCA TTTTGCTGCC GAGGACGTGA AGGCCAAGCT CCATGAACTG 
AACCAGAAGT GGGAGTCGCT GAAGTCCAAG GCCTCCCAGC GACGGCAGGA CCTGGAGGAC 
TCGCTGCAGG CCCAGCAGTA CTTCGCTGAT GCCAACGAGG CCGAGTCCTG GATGCGGGAG 
AAGGAGCCCA TCGTGGGCAG CACCGACTAT GGCAAAGATG AGGACTCTGC CGAGGCTCTG 
CTAAAGAAGC ACGAGGCATT GATGTCGGAT CTCAGCGCCT ATGGCAGCAG CATCCAGGCC 
CTGCGAGAGC AGGCCCAGTC GTGCCGGCAA CAAGTGGCCC CCATGGATGA CGAGACTGGG 
AAGGAGCTGG TCTTGGCGCT CTATGACTAT CAGGAGAAGA GTCCCCGAGA GGTCACCATG 
AAGAAAGGAG ACATTCTCAC CTTACTCAAC AGCACCAACA AGGACTGGTG GAAAGTGGAA 
GTGAACGACC GGCAGGGCTT CGTGCCGGCC GCCTACGTGA AGAAGCTGGA CCCAGCGCAG 
TCGGCCTCCC GGGAGAACCT CCTGGAAGAG CAGGGCAGCA TCGCGCTGCG GCAGGAGCAG 
ATTGACAACC AGTACCATTC TCTGCTGGAA CTGGGAGAGA AGCGTAAAGG CATGTTGGAG 
AAGAGCTGTA AGAAGTTCAT GCTGTTCCGT GAAGCGAACG AGCTGCAGCA GTGGATCAAC 
GAGAAGGAAG CGGCCCTGAC AAGCGAGGAG GTCGGCGCGG ACTTGGAGCA GGTCGAAGTG 
CTGCAGAAGA AGTTTGACGA CTTCCAGAAG GATCTCAAGG CCAATGAGTC ACGGCTGAAG 
GACATTAACA AGGTGGCTGA AGACCTGGAG TCGGAAGGCC TGATGGCGGA GGAGGTGCAG 
GCCGTGCAGC AGCAGGAGGT GTATGGCGCG ATGCCCAGGG ATGAATCTGA TTCCAAGACA 
GCCTCCCCGT GGAAGTCTGC TCGCCTGATG GTTCACACCG TGGCCACCTT TAATTCCATC 
AAGGAGCTCA ATGAGCGCTG GCGGTCCCTG CAGCAGCTGG CTGAGGAGCG GAGCCAACTC 
TTGGGCAGCG CCCACGAAGT GCAGCGGTTC CACAGAGATG CTGACGAAAC CAAAGAGTGG 
ATCGAGGAGA AGAACCAGGC TCTAAACACA GACAACTATG GCCACGACCT GGCCAGCGTC 
CAGGCCCTGC AGCGCAAGCA TGAGGGCTTC GAGAGGGACC TCGCGGCCCT TGGCGACAAG 
GTGAACTCGC TCGGGGAGAC AGCCGAGCGC CTGATCCAGT CCCACCCCGA GTCGGCAGAG 
GACCTGCAGG AGAAGTGCAC TGAGCTGAAC CAGGCATGGA GCAGCCTGGG CAAGCGTGCC 
GACCAGCGCA AGGCCAAGCT GGGCGACTCC CACGACCTGC AGCGCTTCCT CAGCGACTTC 
CGGGACCTCA TGTCTTGGAT CAATGGAATC CGAGGTCTGG TGTCCTCAGA CGAGCTAGCC 
AAGGATGTCA CTGGAGCTGA GGCCCTGCTG GAGCGACACC AGGAACACCG GACAGAAATT 
GATGCCAGAG CTGGCACTTT CCAGGCATTC GAGCAGTTTG GGCAGCAGCT GTTGGCTCAT 
GGGCACTACG CCAGCCCCGA GATCAAGGAG AAGCTCGATG TTCTAGACCA GGAGCGCGCA 
GACCTCGAGA AGGCCTGGGT CCAGCGCAGG ATGATGCTGG ATCAGTGCCT TGAATTGCAG 
CTGTTCCATC GGGATTGCGA GCAGGCCGAG AACTGGATGG CTGCCCGGGA GGCCTTCCTG 
AACACAGAAG ACAAAGGGGA CTCGCTAGAC AGCGTGGAGG CTCTGATCAA AAAACACGAG 
GACTTTGACA AAGCAATTAA TGTCCAGGAG GAGAAGATTG CTGCCCTGCA GTCCTTTGCT 
GACCAGCTCA TTGCTGGCGG TCACTACGCC AAAGGCGACA TCTCCAGCCG GCGCAATGAG 
GTTCTGGACA GGTGGCGCCG TCTAAAAGCT CAGATGATTG AGAAGAGGTC AAAGCTGGGA 
GAATCGCAGA CCCTCCAGCA GTTCAGCCGG GATGTAGACG AGATTGAAGC TTGGATCAGT 
GAAAAATTAC AGACTGCAAG TGATGAGTCA TATAAGGACC CCACCAACAT CCAGCTTTCC 
AAGCTGCTGA GCAAGCATCA GAAGCACCAG GCCTTCGAGG CAGAACTGCA CGCCAATGCG 
GACCGGATCC GCGGGGTCAT CGACGTGGGC AACTCCCTCA TTGACCGTGG GGCCTGTGCC 
GGCAGCGAGG ATGCCGTCAA GGCCCGGCTG GCCGCCTTAG CTGACCAGTG GCAGTTCCTG 
GTGCAGAAGT CGGCTGAGAA GAGCCAGAAG CTGAAAGAAG CCAACAAACA GCAGAACTTC 
AACACTGGCA TCAAGGACTT CGACTTCTGG CTCTCTGAGG TGGAAGCCCT GCTGGCCTCT 
GAGGATTATG GCAAAGACCT GGCTTCTGTG AACAACTTGC TAAAAAAACA TCAGCTGCTG 
GAGGCAGATA TCTCTGCCCA CGAGGACCGC CTGAAGGACC TCAACAGCCA GGCGGACAGC 
CTGATGACCA GCAGCGCCTT CGACACCTCC CAGGTGAAGG ATAAGCGGGA CACCATCAAC 
AGCCGTTTCC AGAAGATCAA GAGCATGGCC GCCTCCCGGC GAGCTAGGCT GAATGAGTCC 
CACCGCCTGC ACCAGTTCTT CCGGGACATG GACGATGAGG AGTCCTGGAT CAAGGAGAAG 
AAGCTGCTGG TGAGCTCAGA GGACTACGGC CGCGACCTGA CGGGCGTCCA GAACCTGAGG 
AAGAAGCACA AACGGCTGGA GGCAGAGCTG GCTGCCCACG AGCCGGCCAT TCAGGGTGTC 
CTGGACACTG GAAAGAAGCT GTCTGATGAC AACACCATCG GGAAGGAGGA GATTCAGCAG 
CGCCTGGCGC AGTTTGTGGA GCACTGGAAG GAGCTGAAGC AGCTGGCAGC TGCTCGGGGC 
CAGCGGCTAG AAGAATCTTT GGAATATCAG CAGTTTGTGG CCAACGTGGA GGAGGAGGAA 
GCCTGGATCA ATGAGAAGAT GACCCTGGTG GCCAGTGAGG ATTACGGAGA CACTCTTGCT 
GCCATCCAGG GCTTGCTGAA GAAACACGAA GCCTTCGAGA CGGACTTCAC TGTCCACAAG 
GACCGGGTGA ATGACGTCTG CACTAACGGA CAAGACCTGG TTAAGAAGAA CAATCACCAT 
GAGGAGAACA TCTCTTCCAA GATGAAGGGC TTGAACGGGA AAGTGTCAGA CTTGGAGAAG 
GCTGCCTCCC AGAGGAAGGC AAAGCTCGAC GAGAACTCGG CCTTCCTCCA GTTCAACTGG 
AAGGCCGATG TGGTGGAGTC CTGGATTGGT GAAAAGGAGA ACAGCTTGAA GACAGATGAT 
TATGGTCGAG ATCTGTCTTC TGTCCAAACT CTCCTCACCA AACAGGAAAC CTTCGATGCT 
GGCCTGCAGG CCTTCCAGCA GGAGGGCATC GCCAACATCA CTGCCCTCAA AGATCAGCTG 
CTGGCCGCCA AGCATGTTCA GTCCAAGGCC ATCGAGGCCC GCCACGCTGC CCTCATGAAG 
AGATGGAGCC AGCTCCTGGC CAATTCGGCC ACCCGGAAGA AGAAGCTGCT GGAGGCACAG 
AGTCACTTCC GCAAGGTGGA GGATCTCTTC CTGACCTTTG CCAAAAAGGC TTCAGCCTTC 
AACAGCTGGT TTGAAAATGC AGAGGAGGAC TTAACAGACC CAGTGCGCTG CAACTCCCTG 
GAAGAGATCA AGGCCTTGAG AGAGGCCCAC GATGCCTTCC GCTCCTCGCT GAGCTCAGCC 
CAGGCCGACT TCAACCAGCT GGCTGAGCTG GACCGCCAGA TCAAGAGCTT CCGCGTGGCC 
TCCAACCCCT ACACCTGGTT CACCATGGAG GCCCTGGAGG AGACCTGGAG GAACCTTCAG 
AAGATCATCA AGGAAAGGGA GCTGGAGCTG CAGAAGGAGC AGCGGCGGCA GGAGGAGAAC 
GACAAGCTGC GCCAGGAGTT CGCCCAGCAT GCCAACGCCT TCCACCAGTG GATCCAGGAG 
ACCAGAACGT ATCTCCTCGA TGGCATAGCG TATCGTCGGG TCATTCGTGT CTATCAGTAT 
GAAGTTGGGG ATGATCTGTC TGGAAGGTCC TGCATGGTGG AAGAATCTGG GACCCTTGAA 
TCGCAGCTTG AAGCTACCAA ACGCAAACAC CAGGAGATTC GAGCCATGAG AAGTCAGCTC 
AAGAAGATTG AAGACCTGGG GGCGGCCATG GAGGAAGCGC TGATCCTGGA CAACAAGTAC 
ACGGAGCACA GCACCGTGGG CCTGGCCCAG CAGTGGGACC AGCTCGACCA GCTGGGCATG 
CGGATGCAGC ACAACCTGGA GCAGCAGATC CAGGCCAGGA ACACGACAGG AGTGACCGAG 
GAGGCCCTCA AAGAATTCAG CATGATGTTC AAACACTTCG ACAAGGACAA GTCCGGCCGG 
CTGAATCACC AGGAGTTCAA ATCCTGTCTG CGCTCCCTGG GCTATGACCT GCCCATGGTG 
GAGGAAGGGG AGCCCGACCC TGAGTTCGAG GCCATCTTGG ATACTGTGGA TCCTAACAGG 
GACGGCCACG TCTCCCTGCA AGAGTACATG GCCTTTATGA TCAGCCGTGA GACCGAGAAT 
GTCAAGTCCA GCGAGGAGAT TGAGAGCGCC TTCCGGGCCC TGAGCTCCGA AGGGAAGCCT 
TATGTGACCA AGGAGGAGCT GTACCAGAAC CTGACTCGGG AACAAGCCGA CTACTGCGTC 
TCCCACATGA AGCCCTACGT GGACGGCAAG GGCCGCGAAC TCCCCACTGC TTTCGACTAC 
GTTGAGTTCA CCCGCTCGCT CTTCGTGAAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012002873.1
CDS	82..7554
Translation	MLSSFRKRRVQKMDPSGVKVLETAEDIQERRQQVLDRYHRFKELSTLRRQKLEDSYRFQFFQRDAEELEKWIQEKLQIASDENYKDPTNLQGKLQKHQAFEAEVQANSGAIVKLDETGNLMISEGHFASETIRTRLMELHRQWELLLEKMREKGIKLLQAQKLVQYLRECEDVMDWINDKEAIVTSEELGQDLEHVEVLQKKFEEFQTDMAAHEERVNEVNQFAAKLIQEQHPEEELIKTKEDEVNAAWQRLKGLALQRQGKLFGAAEVQRFNRDVDETISWIKEKEQLMASDDFGRDLASVQALLRKHEGLERDLAALEDKVKALCAEADRLQQSHPLSATQIQVKREELITNWEQIRTLAAERHARLNDSYRLQRFLADFRDLTSWVTEMKALINADELANDVAGAEALLDRHQEHKGEIDAHEDSFKSADESGQALLAAGHYASDEVREKLTILSEERAALLELWELRRQQYEQCMDLQLFYRDTEQVDNWMSKQEAFLLNEDLGDSLDSVEALLKKHEDFEKSLSAQEEKITALDEFATKLIQNNHYAMEDVATRRDALLSRRNALHERAMYRRAQLADSFHLQQFFRDSDELKSWVNEKMKTATDEAYKDPSNLQGKVQKHQAFEAELSANQSRIDALEKAGQKLIDVDHYAKDEVATRMNEVISLWKKLLEATELKGIKLREANQQQQFNRNVEDIELWLYEVEGHLASDDYGKDLTNVQNLQKKHALLEADVAAHQDRIDGITIQARQFQDAGHFDAENIKKKQEALVARYEALKEPMVARKQKLADSLRLQQLFRDVEDEETWIREKEPIAASTNRGKDLIGVQNLLKKHQALQAEIAGHEPRIKAVTQKGNAMVEEGHFAAEDVKAKLHELNQKWESLKSKASQRRQDLEDSLQAQQYFADANEAESWMREKEPIVGSTDYGKDEDSAEALLKKHEALMSDLSAYGSSIQALREQAQSCRQQVAPMDDETGKELVLALYDYQEKSPREVTMKKGDILTLLNSTNKDWWKVEVNDRQGFVPAAYVKKLDPAQSASRENLLEEQGSIALRQEQIDNQYHSLLELGEKRKGMLEKSCKKFMLFREANELQQWINEKEAALTSEEVGADLEQVEVLQKKFDDFQKDLKANESRLKDINKVAEDLESEGLMAEEVQAVQQQEVYGAMPRDESDSKTASPWKSARLMVHTVATFNSIKELNERWRSLQQLAEERSQLLGSAHEVQRFHRDADETKEWIEEKNQALNTDNYGHDLASVQALQRKHEGFERDLAALGDKVNSLGETAERLIQSHPESAEDLQEKCTELNQAWSSLGKRADQRKAKLGDSHDLQRFLSDFRDLMSWINGIRGLVSSDELAKDVTGAEALLERHQEHRTEIDARAGTFQAFEQFGQQLLAHGHYASPEIKEKLDVLDQERADLEKAWVQRRMMLDQCLELQLFHRDCEQAENWMAAREAFLNTEDKGDSLDSVEALIKKHEDFDKAINVQEEKIAALQSFADQLIAGGHYAKGDISSRRNEVLDRWRRLKAQMIEKRSKLGESQTLQQFSRDVDEIEAWISEKLQTASDESYKDPTNIQLSKLLSKHQKHQAFEAELHANADRIRGVIDVGNSLIDRGACAGSEDAVKARLAALADQWQFLVQKSAEKSQKLKEANKQQNFNTGIKDFDFWLSEVEALLASEDYGKDLASVNNLLKKHQLLEADISAHEDRLKDLNSQADSLMTSSAFDTSQVKDKRDTINSRFQKIKSMAASRRARLNESHRLHQFFRDMDDEESWIKEKKLLVSSEDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQFVEHWKELKQLAAARGQRLEESLEYQQFVANVEEEEAWINEKMTLVASEDYGDTLAAIQGLLKKHEAFETDFTVHKDRVNDVCTNGQDLVKKNNHHEENISSKMKGLNGKVSDLEKAASQRKAKLDENSAFLQFNWKADVVESWIGEKENSLKTDDYGRDLSSVQTLLTKQETFDAGLQAFQQEGIANITALKDQLLAAKHVQSKAIEARHAALMKRWSQLLANSATRKKKLLEAQSHFRKVEDLFLTFAKKASAFNSWFENAEEDLTDPVRCNSLEEIKALREAHDAFRSSLSSAQADFNQLAELDRQIKSFRVASNPYTWFTMEALEETWRNLQKIIKERELELQKEQRRQEENDKLRQEFAQHANAFHQWIQETRTYLLDGIAYRRVIRVYQYEVGDDLSGRSCMVEESGTLESQLEATKRKHQEIRAMRSQLKKIEDLGAAMEEALILDNKYTEHSTVGLAQQWDQLDQLGMRMQHNLEQQIQARNTTGVTEEALKEFSMMFKHFDKDKSGRLNHQEFKSCLRSLGYDLPMVEEGEPDPEFEAILDTVDPNRDGHVSLQEYMAFMISRETENVKSSEEIESAFRALSSEGKPYVTKEELYQNLTREQADYCVSHMKPYVDGKGRELPTAFDYVEFTRSLFVN

Target ORF information:

RefSeq Version	XM_012147483.1
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon spectrin, alpha, non-erythrocytic 1 (SPTAN1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012147483.1

ORF Insert Sequence:

ATGCTGTCAT CGTTTCGTAA ACGCAGAGTC CAGAAAATGG ACCCAAGCGG GGTCAAAGTG 
CTGGAGACAG CCGAGGACAT CCAGGAGAGG CGGCAGCAGG TCCTGGACAG ATACCACCGC 
TTCAAGGAGC TCTCGACCCT GAGGCGCCAG AAGCTTGAAG ACTCCTACCG ATTCCAGTTT 
TTCCAAAGAG ATGCTGAGGA GCTGGAGAAA TGGATTCAGG AGAAGCTTCA GATTGCCTCG 
GATGAGAATT ACAAAGACCC AACCAACCTG CAGGGCAAGC TGCAGAAGCA TCAAGCCTTC 
GAAGCCGAGG TGCAGGCCAA CTCGGGTGCC ATCGTGAAGC TTGATGAGAC CGGTAACCTG 
ATGATCTCAG AAGGGCACTT CGCGTCCGAA ACCATCCGGA CTCGTCTGAT GGAGCTGCAC 
CGCCAGTGGG AATTGCTTCT GGAGAAGATG CGGGAGAAAG GCATCAAGCT GCTGCAGGCC 
CAGAAGCTGG TGCAGTACCT GCGGGAGTGT GAGGACGTGA TGGACTGGAT CAATGACAAG 
GAAGCCATTG TGACATCTGA GGAGCTGGGC CAGGACCTGG AGCACGTGGA GGTGTTACAG 
AAGAAATTCG AAGAGTTTCA GACAGACATG GCTGCCCACG AAGAGAGAGT CAATGAAGTG 
AACCAGTTTG CTGCCAAGCT CATTCAGGAG CAGCACCCTG AGGAGGAGCT GATCAAGACA 
AAGGAGGACG AGGTGAATGC AGCCTGGCAG CGGCTGAAGG GCCTGGCCCT CCAGCGGCAG 
GGGAAGCTGT TCGGGGCAGC TGAGGTCCAA CGCTTTAACA GGGATGTGGA CGAGACCATT 
AGCTGGATTA AGGAGAAGGA ACAGTTAATG GCCTCTGATG ACTTTGGCCG GGACCTGGCC 
AGTGTCCAGG CTCTACTGCG GAAACACGAG GGTTTGGAGA GAGATCTTGC TGCCTTGGAG 
GACAAGGTCA AAGCCCTGTG TGCCGAGGCT GACCGCCTGC AGCAGTCCCA CCCTCTGAGT 
GCCACCCAGA TCCAAGTGAA GCGAGAGGAG CTGATCACCA ACTGGGAGCA GATCCGCACC 
CTGGCCGCGG AGAGACACGC ACGTCTCAAC GATTCCTACA GGCTTCAGCG CTTCCTTGCT 
GACTTCCGAG ACCTCACCAG CTGGGTCACT GAGATGAAAG CCCTCATCAA TGCAGATGAA 
CTTGCCAATG ATGTGGCCGG GGCCGAAGCC CTGCTGGATA GGCATCAGGA GCACAAGGGT 
GAAATTGACG CTCATGAAGA TAGCTTTAAA TCTGCAGATG AGTCTGGACA GGCCCTGCTT 
GCAGCTGGTC ACTATGCCTC AGATGAAGTG AGGGAGAAGC TGACCATCCT GTCCGAGGAG 
CGGGCCGCGC TACTAGAGCT CTGGGAGCTG CGACGGCAGC AATATGAGCA GTGCATGGAC 
CTGCAGCTCT TCTACCGCGA CACGGAGCAG GTGGACAACT GGATGAGCAA GCAGGAGGCG 
TTCCTGTTGA ACGAAGACCT GGGTGATTCC TTGGATAGTG TGGAAGCGCT TCTCAAGAAG 
CACGAAGACT TTGAGAAGTC CCTCAGCGCC CAGGAGGAGA AGATCACAGC CTTAGATGAG 
TTTGCCACCA AGCTGATTCA GAACAACCAC TACGCGATGG AAGATGTGGC CACCCGGCGA 
GACGCACTGC TGAGCCGCCG CAACGCCCTC CATGAGAGAG CCATGTACCG CCGTGCCCAG 
CTTGCAGATT CCTTCCACTT GCAGCAGTTT TTCCGTGACT CTGATGAGCT CAAGAGTTGG 
GTCAACGAGA AGATGAAAAC AGCCACAGAT GAAGCTTATA AAGACCCATC CAACCTACAA 
GGGAAAGTGC AGAAGCACCA GGCCTTTGAG GCCGAGCTCT CAGCAAACCA GAGCCGCATC 
GACGCCTTGG AGAAAGCTGG GCAGAAGCTG ATCGACGTTG ACCACTATGC CAAGGATGAG 
GTGGCGACTC GGATGAATGA AGTTATCAGT CTGTGGAAGA AGCTGCTGGA GGCCACGGAG 
CTGAAAGGCA TAAAGCTCCG TGAGGCTAAC CAGCAGCAGC AGTTTAACCG CAATGTTGAG 
GACATCGAGC TGTGGCTGTA CGAAGTGGAA GGTCATCTGG CCTCGGATGA CTACGGCAAA 
GACCTCACCA ACGTCCAGAA CCTCCAGAAA AAGCATGCCC TGCTGGAGGC TGACGTGGCC 
GCTCACCAGG ATCGCATCGA TGGCATCACT ATCCAAGCCC GCCAGTTCCA GGATGCGGGC 
CACTTTGATG CTGAAAACAT CAAGAAGAAG CAGGAGGCCC TGGTAGCTCG CTACGAGGCA 
CTCAAGGAGC CCATGGTTGC CCGGAAGCAG AAGCTGGCCG ATTCCCTGCG GTTGCAGCAG 
CTCTTCCGTG ACGTTGAGGA TGAGGAGACG TGGATTCGGG AGAAAGAACC TATTGCCGCC 
TCCACCAACA GAGGCAAAGA TTTAATTGGA GTCCAGAACC TGCTAAAGAA ACACCAAGCG 
CTACAAGCAG AAATTGCTGG CCACGAGCCC CGCATCAAAG CAGTCACACA GAAGGGGAAC 
GCCATGGTAG AGGAGGGCCA TTTTGCTGCC GAGGACGTGA AGGCCAAGCT CCATGAACTG 
AACCAGAAGT GGGAGTCGCT GAAGTCCAAG GCCTCCCAGC GACGGCAGGA CCTGGAGGAC 
TCGCTGCAGG CCCAGCAGTA CTTCGCTGAT GCCAACGAGG CCGAGTCCTG GATGCGGGAG 
AAGGAGCCCA TCGTGGGCAG CACCGACTAT GGCAAAGATG AGGACTCTGC CGAGGCTCTG 
CTAAAGAAGC ACGAGGCATT GATGTCGGAT CTCAGCGCCT ATGGCAGCAG CATCCAGGCC 
CTGCGAGAGC AGGCCCAGTC GTGCCGGCAA CAAGTGGCCC CCATGGATGA CGAGACTGGG 
AAGGAGCTGG TCTTGGCGCT CTATGACTAT CAGGAGAAGA GTCCCCGAGA GGTCACCATG 
AAGAAAGGAG ACATTCTCAC CTTACTCAAC AGCACCAACA AGGACTGGTG GAAAGTGGAA 
GTGAACGACC GGCAGGGCTT CGTGCCGGCC GCCTACGTGA AGAAGCTGGA CCCAGCGCAG 
TCGGCCTCCC GGGAGAACCT CCTGGAAGAG CAGGGCAGCA TCGCGCTGCG GCAGGAGCAG 
ATTGACAACC AGTACCATTC TCTGCTGGAA CTGGGAGAGA AGCGTAAAGG CATGTTGGAG 
AAGAGCTGTA AGAAGTTCAT GCTGTTCCGT GAAGCGAACG AGCTGCAGCA GTGGATCAAC 
GAGAAGGAAG CGGCCCTGAC AAGCGAGGAG GTCGGCGCGG ACTTGGAGCA GGTCGAAGTG 
CTGCAGAAGA AGTTTGACGA CTTCCAGAAG GATCTCAAGG CCAATGAGTC ACGGCTGAAG 
GACATTAACA AGGTGGCTGA AGACCTGGAG TCGGAAGGCC TGATGGCGGA GGAGGTGCAG 
GCCGTGCAGC AGCAGGAGGT GTATGGCGCG ATGCCCAGGG ATGAATCTGA TTCCAAGACA 
GCCTCCCCGT GGAAGTCTGC TCGCCTGATG GTTCACACCG TGGCCACCTT TAATTCCATC 
AAGGAGCTCA ATGAGCGCTG GCGGTCCCTG CAGCAGCTGG CTGAGGAGCG GAGCCAACTC 
TTGGGCAGCG CCCACGAAGT GCAGCGGTTC CACAGAGATG CTGACGAAAC CAAAGAGTGG 
ATCGAGGAGA AGAACCAGGC TCTAAACACA GACAACTATG GCCACGACCT GGCCAGCGTC 
CAGGCCCTGC AGCGCAAGCA TGAGGGCTTC GAGAGGGACC TCGCGGCCCT TGGCGACAAG 
GTGAACTCGC TCGGGGAGAC AGCCGAGCGC CTGATCCAGT CCCACCCCGA GTCGGCAGAG 
GACCTGCAGG AGAAGTGCAC TGAGCTGAAC CAGGCATGGA GCAGCCTGGG CAAGCGTGCC 
GACCAGCGCA AGGCCAAGCT GGGCGACTCC CACGACCTGC AGCGCTTCCT CAGCGACTTC 
CGGGACCTCA TGTCTTGGAT CAATGGAATC CGAGGTCTGG TGTCCTCAGA CGAGCTAGCC 
AAGGATGTCA CTGGAGCTGA GGCCCTGCTG GAGCGACACC AGGAACACCG GACAGAAATT 
GATGCCAGAG CTGGCACTTT CCAGGCATTC GAGCAGTTTG GGCAGCAGCT GTTGGCTCAT 
GGGCACTACG CCAGCCCCGA GATCAAGGAG AAGCTCGATG TTCTAGACCA GGAGCGCGCA 
GACCTCGAGA AGGCCTGGGT CCAGCGCAGG ATGATGCTGG ATCAGTGCCT TGAATTGCAG 
CTGTTCCATC GGGATTGCGA GCAGGCCGAG AACTGGATGG CTGCCCGGGA GGCCTTCCTG 
AACACAGAAG ACAAAGGGGA CTCGCTAGAC AGCGTGGAGG CTCTGATCAA AAAACACGAG 
GACTTTGACA AAGCAATTAA TGTCCAGGAG GAGAAGATTG CTGCCCTGCA GTCCTTTGCT 
GACCAGCTCA TTGCTGGCGG TCACTACGCC AAAGGCGACA TCTCCAGCCG GCGCAATGAG 
GTTCTGGACA GGTGGCGCCG TCTAAAAGCT CAGATGATTG AGAAGAGGTC AAAGCTGGGA 
GAATCGCAGA CCCTCCAGCA GTTCAGCCGG GATGTAGACG AGATTGAAGC TTGGATCAGT 
GAAAAATTAC AGACTGCAAG TGATGAGTCA TATAAGGACC CCACCAACAT CCAGCTTTCC 
AAGCTGCTGA GCAAGCATCA GAAGCACCAG GCCTTCGAGG CAGAACTGCA CGCCAATGCG 
GACCGGATCC GCGGGGTCAT CGACGTGGGC AACTCCCTCA TTGACCGTGG GGCCTGTGCC 
GGCAGCGAGG ATGCCGTCAA GGCCCGGCTG GCCGCCTTAG CTGACCAGTG GCAGTTCCTG 
GTGCAGAAGT CGGCTGAGAA GAGCCAGAAG CTGAAAGAAG CCAACAAACA GCAGAACTTC 
AACACTGGCA TCAAGGACTT CGACTTCTGG CTCTCTGAGG TGGAAGCCCT GCTGGCCTCT 
GAGGATTATG GCAAAGACCT GGCTTCTGTG AACAACTTGC TAAAAAAACA TCAGCTGCTG 
GAGGCAGATA TCTCTGCCCA CGAGGACCGC CTGAAGGACC TCAACAGCCA GGCGGACAGC 
CTGATGACCA GCAGCGCCTT CGACACCTCC CAGGTGAAGG ATAAGCGGGA CACCATCAAC 
AGCCGTTTCC AGAAGATCAA GAGCATGGCC GCCTCCCGGC GAGCTAGGCT GAATGAGTCC 
CACCGCCTGC ACCAGTTCTT CCGGGACATG GACGATGAGG AGTCCTGGAT CAAGGAGAAG 
AAGCTGCTGG TGAGCTCAGA GGACTACGGC CGCGACCTGA CGGGCGTCCA GAACCTGAGG 
AAGAAGCACA AACGGCTGGA GGCAGAGCTG GCTGCCCACG AGCCGGCCAT TCAGGGTGTC 
CTGGACACTG GAAAGAAGCT GTCTGATGAC AACACCATCG GGAAGGAGGA GATTCAGCAG 
CGCCTGGCGC AGTTTGTGGA GCACTGGAAG GAGCTGAAGC AGCTGGCAGC TGCTCGGGGC 
CAGCGGCTAG AAGAATCTTT GGAATATCAG CAGTTTGTGG CCAACGTGGA GGAGGAGGAA 
GCCTGGATCA ATGAGAAGAT GACCCTGGTG GCCAGTGAGG ATTACGGAGA CACTCTTGCT 
GCCATCCAGG GCTTGCTGAA GAAACACGAA GCCTTCGAGA CGGACTTCAC TGTCCACAAG 
GACCGGGTGA ATGACGTCTG CACTAACGGA CAAGACCTGG TTAAGAAGAA CAATCACCAT 
GAGGAGAACA TCTCTTCCAA GATGAAGGGC TTGAACGGGA AAGTGTCAGA CTTGGAGAAG 
GCTGCCTCCC AGAGGAAGGC AAAGCTCGAC GAGAACTCGG CCTTCCTCCA GTTCAACTGG 
AAGGCCGATG TGGTGGAGTC CTGGATTGGT GAAAAGGAGA ACAGCTTGAA GACAGATGAT 
TATGGTCGAG ATCTGTCTTC TGTCCAAACT CTCCTCACCA AACAGGAAAC CTTCGATGCT 
GGCCTGCAGG CCTTCCAGCA GGAGGGCATC GCCAACATCA CTGCCCTCAA AGATCAGCTG 
CTGGCCGCCA AGCATGTTCA GTCCAAGGCC ATCGAGGCCC GCCACGCTGC CCTCATGAAG 
AGATGGAGCC AGCTCCTGGC CAATTCGGCC ACCCGGAAGA AGAAGCTGCT GGAGGCACAG 
AGTCACTTCC GCAAGGTGGA GGATCTCTTC CTGACCTTTG CCAAAAAGGC TTCAGCCTTC 
AACAGCTGGT TTGAAAATGC AGAGGAGGAC TTAACAGACC CAGTGCGCTG CAACTCCCTG 
GAAGAGATCA AGGCCTTGAG AGAGGCCCAC GATGCCTTCC GCTCCTCGCT GAGCTCAGCC 
CAGGCCGACT TCAACCAGCT GGCTGAGCTG GACCGCCAGA TCAAGAGCTT CCGCGTGGCC 
TCCAACCCCT ACACCTGGTT CACCATGGAG GCCCTGGAGG AGACCTGGAG GAACCTTCAG 
AAGATCATCA AGGAAAGGGA GCTGGAGCTG CAGAAGGAGC AGCGGCGGCA GGAGGAGAAC 
GACAAGCTGC GCCAGGAGTT CGCCCAGCAT GCCAACGCCT TCCACCAGTG GATCCAGGAG 
ACCAGAACGT ATCTCCTCGA TGGCATAGCG TATCGTCGGG TCATTCGTGT CTATCAGTAT 
GAAGTTGGGG ATGATCTGTC TGGAAGGTCC TGCATGGTGG AAGAATCTGG GACCCTTGAA 
TCGCAGCTTG AAGCTACCAA ACGCAAACAC CAGGAGATTC GAGCCATGAG AAGTCAGCTC 
AAGAAGATTG AAGACCTGGG GGCGGCCATG GAGGAAGCGC TGATCCTGGA CAACAAGTAC 
ACGGAGCACA GCACCGTGGG CCTGGCCCAG CAGTGGGACC AGCTCGACCA GCTGGGCATG 
CGGATGCAGC ACAACCTGGA GCAGCAGATC CAGGCCAGGA ACACGACAGG AGTGACCGAG 
GAGGCCCTCA AAGAATTCAG CATGATGTTC AAACACTTCG ACAAGGACAA GTCCGGCCGG 
CTGAATCACC AGGAGTTCAA ATCCTGTCTG CGCTCCCTGG GCTATGACCT GCCCATGGTG 
GAGGAAGGGG AGCCCGACCC TGAGTTCGAG GCCATCTTGG ATACTGTGGA TCCTAACAGG 
GACGGCCACG TCTCCCTGCA AGAGTACATG GCCTTTATGA TCAGCCGTGA GACCGAGAAT 
GTCAAGTCCA GCGAGGAGAT TGAGAGCGCC TTCCGGGCCC TGAGCTCCGA AGGGAAGCCT 
TATGTGACCA AGGAGGAGCT GTACCAGAAC CTGACTCGGG AACAAGCCGA CTACTGCGTC 
TCCCACATGA AGCCCTACGT GGACGGCAAG GGCCGCGAAC TCCCCACTGC TTTCGACTAC 
GTTGAGTTCA CCCGCTCGCT CTTCGTGAAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTAN1 ( XM_012147484.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_012002874.1 Ovis aries musimon spectrin, alpha, non-erythrocytic 1 (SPTAN1), transcript variant X8, mRNA.

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CloneID	OOv24454
Clone ID Related Accession (Same CDS sequence)	XM_012147484.1
Accession Version	XM_012147484.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7470bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	spectrin alpha chain, non-erythrocytic 1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942583.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTCAT CGTTTCGTAA ACGCAGAGTC CAGAAAATGG ACCCAAGCGG GGTCAAAGTG 
CTGGAGACAG CCGAGGACAT CCAGGAGAGG CGGCAGCAGG TCCTGGACAG ATACCACCGC 
TTCAAGGAGC TCTCGACCCT GAGGCGCCAG AAGCTTGAAG ACTCCTACCG ATTCCAGTTT 
TTCCAAAGAG ATGCTGAGGA GCTGGAGAAA TGGATTCAGG AGAAGCTTCA GATTGCCTCG 
GATGAGAATT ACAAAGACCC AACCAACCTG CAGGGCAAGC TGCAGAAGCA TCAAGCCTTC 
GAAGCCGAGG TGCAGGCCAA CTCGGGTGCC ATCGTGAAGC TTGATGAGAC CGGTAACCTG 
ATGATCTCAG AAGGGCACTT CGCGTCCGAA ACCATCCGGA CTCGTCTGAT GGAGCTGCAC 
CGCCAGTGGG AATTGCTTCT GGAGAAGATG CGGGAGAAAG GCATCAAGCT GCTGCAGGCC 
CAGAAGCTGG TGCAGTACCT GCGGGAGTGT GAGGACGTGA TGGACTGGAT CAATGACAAG 
GAAGCCATTG TGACATCTGA GGAGCTGGGC CAGGACCTGG AGCACGTGGA GGTGTTACAG 
AAGAAATTCG AAGAGTTTCA GACAGACATG GCTGCCCACG AAGAGAGAGT CAATGAAGTG 
AACCAGTTTG CTGCCAAGCT CATTCAGGAG CAGCACCCTG AGGAGGAGCT GATCAAGACA 
AAGGAGGACG AGGTGAATGC AGCCTGGCAG CGGCTGAAGG GCCTGGCCCT CCAGCGGCAG 
GGGAAGCTGT TCGGGGCAGC TGAGGTCCAA CGCTTTAACA GGGATGTGGA CGAGACCATT 
AGCTGGATTA AGGAGAAGGA ACAGTTAATG GCCTCTGATG ACTTTGGCCG GGACCTGGCC 
AGTGTCCAGG CTCTACTGCG GAAACACGAG GGTTTGGAGA GAGATCTTGC TGCCTTGGAG 
GACAAGGTCA AAGCCCTGTG TGCCGAGGCT GACCGCCTGC AGCAGTCCCA CCCTCTGAGT 
GCCACCCAGA TCCAAGTGAA GCGAGAGGAG CTGATCACCA ACTGGGAGCA GATCCGCACC 
CTGGCCGCGG AGAGACACGC ACGTCTCAAC GATTCCTACA GGCTTCAGCG CTTCCTTGCT 
GACTTCCGAG ACCTCACCAG CTGGGTCACT GAGATGAAAG CCCTCATCAA TGCAGATGAA 
CTTGCCAATG ATGTGGCCGG GGCCGAAGCC CTGCTGGATA GGCATCAGGA GCACAAGGGT 
GAAATTGACG CTCATGAAGA TAGCTTTAAA TCTGCAGATG AGTCTGGACA GGCCCTGCTT 
GCAGCTGGTC ACTATGCCTC AGATGAAGTG AGGGAGAAGC TGACCATCCT GTCCGAGGAG 
CGGGCCGCGC TACTAGAGCT CTGGGAGCTG CGACGGCAGC AATATGAGCA GTGCATGGAC 
CTGCAGCTCT TCTACCGCGA CACGGAGCAG GTGGACAACT GGATGAGCAA GCAGGAGGCG 
TTCCTGTTGA ACGAAGACCT GGGTGATTCC TTGGATAGTG TGGAAGCGCT TCTCAAGAAG 
CACGAAGACT TTGAGAAGTC CCTCAGCGCC CAGGAGGAGA AGATCACAGC CTTAGATGAG 
TTTGCCACCA AGCTGATTCA GAACAACCAC TACGCGATGG AAGATGTGGC CACCCGGCGA 
GACGCACTGC TGAGCCGCCG CAACGCCCTC CATGAGAGAG CCATGTACCG CCGTGCCCAG 
CTTGCAGATT CCTTCCACTT GCAGCAGTTT TTCCGTGACT CTGATGAGCT CAAGAGTTGG 
GTCAACGAGA AGATGAAAAC AGCCACAGAT GAAGCTTATA AAGACCCATC CAACCTACAA 
GGGAAAGTGC AGAAGCACCA GGCCTTTGAG GCCGAGCTCT CAGCAAACCA GAGCCGCATC 
GACGCCTTGG AGAAAGCTGG GCAGAAGCTG ATCGACGTTG ACCACTATGC CAAGGATGAG 
GTGGCGACTC GGATGAATGA AGTTATCAGT CTGTGGAAGA AGCTGCTGGA GGCCACGGAG 
CTGAAAGGCA TAAAGCTCCG TGAGGCTAAC CAGCAGCAGC AGTTTAACCG CAATGTTGAG 
GACATCGAGC TGTGGCTGTA CGAAGTGGAA GGTCATCTGG CCTCGGATGA CTACGGCAAA 
GACCTCACCA ACGTCCAGAA CCTCCAGAAA AAGCATGCCC TGCTGGAGGC TGACGTGGCC 
GCTCACCAGG ATCGCATCGA TGGCATCACT ATCCAAGCCC GCCAGTTCCA GGATGCGGGC 
CACTTTGATG CTGAAAACAT CAAGAAGAAG CAGGAGGCCC TGGTAGCTCG CTACGAGGCA 
CTCAAGGAGC CCATGGTTGC CCGGAAGCAG AAGCTGGCCG ATTCCCTGCG GTTGCAGCAG 
CTCTTCCGTG ACGTTGAGGA TGAGGAGACG TGGATTCGGG AGAAAGAACC TATTGCCGCC 
TCCACCAACA GAGGCAAAGA TTTAATTGGA GTCCAGAACC TGCTAAAGAA ACACCAAGCG 
CTACAAGCAG AAATTGCTGG CCACGAGCCC CGCATCAAAG CAGTCACACA GAAGGGGAAC 
GCCATGGTAG AGGAGGGCCA TTTTGCTGCC GAGGACGTGA AGGCCAAGCT CCATGAACTG 
AACCAGAAGT GGGAGTCGCT GAAGTCCAAG GCCTCCCAGC GACGGCAGGA CCTGGAGGAC 
TCGCTGCAGG CCCAGCAGTA CTTCGCTGAT GCCAACGAGG CCGAGTCCTG GATGCGGGAG 
AAGGAGCCCA TCGTGGGCAG CACCGACTAT GGCAAAGATG AGGACTCTGC CGAGGCTCTG 
CTAAAGAAGC ACGAGGCATT GATGTCGGAT CTCAGCGCCT ATGGCAGCAG CATCCAGGCC 
CTGCGAGAGC AGGCCCAGTC GTGCCGGCAA CAAGTGGCCC CCATGGATGA CGAGACTGGG 
AAGGAGCTGG TCTTGGCGCT CTATGACTAT CAGGAGAAGA GTCCCCGAGA GGTCACCATG 
AAGAAAGGAG ACATTCTCAC CTTACTCAAC AGCACCAACA AGGACTGGTG GAAAGTGGAA 
GTGAACGACC GGCAGGGCTT CGTGCCGGCC GCCTACGTGA AGAAGCTGGA CCCAGCGCAG 
TCGGCCTCCC GGGAGAACCT CCTGGAAGAG CAGGGCAGCA TCGCGCTGCG GCAGGAGCAG 
ATTGACAACC AGACGCGCGT AACTAAGGAG GCCGGCAGCG TGTCTCTGCG TATGAAACAG 
GTGGAAGAAC TGTACCATTC TCTGCTGGAA CTGGGAGAGA AGCGTAAAGG CATGTTGGAG 
AAGAGCTGTA AGAAGTTCAT GCTGTTCCGT GAAGCGAACG AGCTGCAGCA GTGGATCAAC 
GAGAAGGAAG CGGCCCTGAC AAGCGAGGAG GTCGGCGCGG ACTTGGAGCA GGTCGAAGTG 
CTGCAGAAGA AGTTTGACGA CTTCCAGAAG GATCTCAAGG CCAATGAGTC ACGGCTGAAG 
GACATTAACA AGGTGGCTGA AGACCTGGAG TCGGAAGGCC TGATGGCGGA GGAGGTGCAG 
GCCGTGCAGC AGCAGGAGGT GTATGGCGCG ATGCCCAGGG ATGAATCTGA TTCCAAGACA 
GCCTCCCCGT GGAAGTCTGC TCGCCTGATG GTTCACACCG TGGCCACCTT TAATTCCATC 
AAGGAGCTCA ATGAGCGCTG GCGGTCCCTG CAGCAGCTGG CTGAGGAGCG GAGCCAACTC 
TTGGGCAGCG CCCACGAAGT GCAGCGGTTC CACAGAGATG CTGACGAAAC CAAAGAGTGG 
ATCGAGGAGA AGAACCAGGC TCTAAACACA GACAACTATG GCCACGACCT GGCCAGCGTC 
CAGGCCCTGC AGCGCAAGCA TGAGGGCTTC GAGAGGGACC TCGCGGCCCT TGGCGACAAG 
GTGAACTCGC TCGGGGAGAC AGCCGAGCGC CTGATCCAGT CCCACCCCGA GTCGGCAGAG 
GACCTGCAGG AGAAGTGCAC TGAGCTGAAC CAGGCATGGA GCAGCCTGGG CAAGCGTGCC 
GACCAGCGCA AGGCCAAGCT GGGCGACTCC CACGACCTGC AGCGCTTCCT CAGCGACTTC 
CGGGACCTCA TGTCTTGGAT CAATGGAATC CGAGGTCTGG TGTCCTCAGA CGAGCTAGCC 
AAGGATGTCA CTGGAGCTGA GGCCCTGCTG GAGCGACACC AGGAACACCG GACAGAAATT 
GATGCCAGAG CTGGCACTTT CCAGGCATTC GAGCAGTTTG GGCAGCAGCT GTTGGCTCAT 
GGGCACTACG CCAGCCCCGA GATCAAGGAG AAGCTCGATG TTCTAGACCA GGAGCGCGCA 
GACCTCGAGA AGGCCTGGGT CCAGCGCAGG ATGATGCTGG ATCAGTGCCT TGAATTGCAG 
CTGTTCCATC GGGATTGCGA GCAGGCCGAG AACTGGATGG CTGCCCGGGA GGCCTTCCTG 
AACACAGAAG ACAAAGGGGA CTCGCTAGAC AGCGTGGAGG CTCTGATCAA AAAACACGAG 
GACTTTGACA AAGCAATTAA TGTCCAGGAG GAGAAGATTG CTGCCCTGCA GTCCTTTGCT 
GACCAGCTCA TTGCTGGCGG TCACTACGCC AAAGGCGACA TCTCCAGCCG GCGCAATGAG 
GTTCTGGACA GGTGGCGCCG TCTAAAAGCT CAGATGATTG AGAAGAGGTC AAAGCTGGGA 
GAATCGCAGA CCCTCCAGCA GTTCAGCCGG GATGTAGACG AGATTGAAGC TTGGATCAGT 
GAAAAATTAC AGACTGCAAG TGATGAGTCA TATAAGGACC CCACCAACAT CCAGCTTTCC 
AAGCTGCTGA GCAAGCATCA GAAGCACCAG GCCTTCGAGG CAGAACTGCA CGCCAATGCG 
GACCGGATCC GCGGGGTCAT CGACGTGGGC AACTCCCTCA TTGACCGTGG GGCCTGTGCC 
GGCAGCGAGG ATGCCGTCAA GGCCCGGCTG GCCGCCTTAG CTGACCAGTG GCAGTTCCTG 
GTGCAGAAGT CGGCTGAGAA GAGCCAGAAG CTGAAAGAAG CCAACAAACA GCAGAACTTC 
AACACTGGCA TCAAGGACTT CGACTTCTGG CTCTCTGAGG TGGAAGCCCT GCTGGCCTCT 
GAGGATTATG GCAAAGACCT GGCTTCTGTG AACAACTTGC TAAAAAAACA TCAGCTGCTG 
GAGGCAGATA TCTCTGCCCA CGAGGACCGC CTGAAGGACC TCAACAGCCA GGCGGACAGC 
CTGATGACCA GCAGCGCCTT CGACACCTCC CAGGTGAAGG ATAAGCGGGA CACCATCAAC 
AGCCGTTTCC AGAAGATCAA GAGCATGGCC GCCTCCCGGC GAGCTAGGCT GAATGAGTCC 
CACCGCCTGC ACCAGTTCTT CCGGGACATG GACGATGAGG AGTCCTGGAT CAAGGAGAAG 
AAGCTGCTGG TGAGCTCAGA GGACTACGGC CGCGACCTGA CGGGCGTCCA GAACCTGAGG 
AAGAAGCACA AACGGCTGGA GGCAGAGCTG GCTGCCCACG AGCCGGCCAT TCAGGGTGTC 
CTGGACACTG GAAAGAAGCT GTCTGATGAC AACACCATCG GGAAGGAGGA GATTCAGCAG 
CGCCTGGCGC AGTTTGTGGA GCACTGGAAG GAGCTGAAGC AGCTGGCAGC TGCTCGGGGC 
CAGCGGCTAG AAGAATCTTT GGAATATCAG CAGTTTGTGG CCAACGTGGA GGAGGAGGAA 
GCCTGGATCA ATGAGAAGAT GACCCTGGTG GCCAGTGAGG ATTACGGAGA CACTCTTGCT 
GCCATCCAGG GCTTGCTGAA GAAACACGAA GCCTTCGAGA CGGACTTCAC TGTCCACAAG 
GACCGGGTGA ATGACGTCTG CACTAACGGA CAAGACCTGG TTAAGAAGAA CAATCACCAT 
GAGGAGAACA TCTCTTCCAA GATGAAGGGC TTGAACGGGA AAGTGTCAGA CTTGGAGAAG 
GCTGCCTCCC AGAGGAAGGC AAAGCTCGAC GAGAACTCGG CCTTCCTCCA GTTCAACTGG 
AAGGCCGATG TGGTGGAGTC CTGGATTGGT GAAAAGGAGA ACAGCTTGAA GACAGATGAT 
TATGGTCGAG ATCTGTCTTC TGTCCAAACT CTCCTCACCA AACAGGAAAC CTTCGATGCT 
GGCCTGCAGG CCTTCCAGCA GGAGGGCATC GCCAACATCA CTGCCCTCAA AGATCAGCTG 
CTGGCCGCCA AGCATGTTCA GTCCAAGGCC ATCGAGGCCC GCCACGCTGC CCTCATGAAG 
AGATGGAGCC AGCTCCTGGC CAATTCGGCC ACCCGGAAGA AGAAGCTGCT GGAGGCACAG 
AGTCACTTCC GCAAGGTGGA GGATCTCTTC CTGACCTTTG CCAAAAAGGC TTCAGCCTTC 
AACAGCTGGT TTGAAAATGC AGAGGAGGAC TTAACAGACC CAGTGCGCTG CAACTCCCTG 
GAAGAGATCA AGGCCTTGAG AGAGGCCCAC GATGCCTTCC GCTCCTCGCT GAGCTCAGCC 
CAGGCCGACT TCAACCAGCT GGCTGAGCTG GACCGCCAGA TCAAGAGCTT CCGCGTGGCC 
TCCAACCCCT ACACCTGGTT CACCATGGAG GCCCTGGAGG AGACCTGGAG GAACCTTCAG 
AAGATCATCA AGGAAAGGGA GCTGGAGCTG CAGAAGGAGC AGCGGCGGCA GGAGGAGAAC 
GACAAGCTGC GCCAGGAGTT CGCCCAGCAT GCCAACGCCT TCCACCAGTG GATCCAGGAG 
ACCAGAACGT ATCTCCTCGA TGGGTCCTGC ATGGTGGAAG AATCTGGGAC CCTTGAATCG 
CAGCTTGAAG CTACCAAACG CAAACACCAG GAGATTCGAG CCATGAGAAG TCAGCTCAAG 
AAGATTGAAG ACCTGGGGGC GGCCATGGAG GAAGCGCTGA TCCTGGACAA CAAGTACACG 
GAGCACAGCA CCGTGGGCCT GGCCCAGCAG TGGGACCAGC TCGACCAGCT GGGCATGCGG 
ATGCAGCACA ACCTGGAGCA GCAGATCCAG GCCAGGAACA CGACAGGAGT GACCGAGGAG 
GCCCTCAAAG AATTCAGCAT GATGTTCAAA CACTTCGACA AGGACAAGTC CGGCCGGCTG 
AATCACCAGG AGTTCAAATC CTGTCTGCGC TCCCTGGGCT ATGACCTGCC CATGGTGGAG 
GAAGGGGAGC CCGACCCTGA GTTCGAGGCC ATCTTGGATA CTGTGGATCC TAACAGGGAC 
GGCCACGTCT CCCTGCAAGA GTACATGGCC TTTATGATCA GCCGTGAGAC CGAGAATGTC 
AAGTCCAGCG AGGAGATTGA GAGCGCCTTC CGGGCCCTGA GCTCCGAAGG GAAGCCTTAT 
GTGACCAAGG AGGAGCTGTA CCAGAACCTG ACTCGGGAAC AAGCCGACTA CTGCGTCTCC 
CACATGAAGC CCTACGTGGA CGGCAAGGGC CGCGAACTCC CCACTGCTTT CGACTACGTT 
GAGTTCACCC GCTCGCTCTT CGTGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012002874.1
CDS	81..7550
Translation	MLSSFRKRRVQKMDPSGVKVLETAEDIQERRQQVLDRYHRFKELSTLRRQKLEDSYRFQFFQRDAEELEKWIQEKLQIASDENYKDPTNLQGKLQKHQAFEAEVQANSGAIVKLDETGNLMISEGHFASETIRTRLMELHRQWELLLEKMREKGIKLLQAQKLVQYLRECEDVMDWINDKEAIVTSEELGQDLEHVEVLQKKFEEFQTDMAAHEERVNEVNQFAAKLIQEQHPEEELIKTKEDEVNAAWQRLKGLALQRQGKLFGAAEVQRFNRDVDETISWIKEKEQLMASDDFGRDLASVQALLRKHEGLERDLAALEDKVKALCAEADRLQQSHPLSATQIQVKREELITNWEQIRTLAAERHARLNDSYRLQRFLADFRDLTSWVTEMKALINADELANDVAGAEALLDRHQEHKGEIDAHEDSFKSADESGQALLAAGHYASDEVREKLTILSEERAALLELWELRRQQYEQCMDLQLFYRDTEQVDNWMSKQEAFLLNEDLGDSLDSVEALLKKHEDFEKSLSAQEEKITALDEFATKLIQNNHYAMEDVATRRDALLSRRNALHERAMYRRAQLADSFHLQQFFRDSDELKSWVNEKMKTATDEAYKDPSNLQGKVQKHQAFEAELSANQSRIDALEKAGQKLIDVDHYAKDEVATRMNEVISLWKKLLEATELKGIKLREANQQQQFNRNVEDIELWLYEVEGHLASDDYGKDLTNVQNLQKKHALLEADVAAHQDRIDGITIQARQFQDAGHFDAENIKKKQEALVARYEALKEPMVARKQKLADSLRLQQLFRDVEDEETWIREKEPIAASTNRGKDLIGVQNLLKKHQALQAEIAGHEPRIKAVTQKGNAMVEEGHFAAEDVKAKLHELNQKWESLKSKASQRRQDLEDSLQAQQYFADANEAESWMREKEPIVGSTDYGKDEDSAEALLKKHEALMSDLSAYGSSIQALREQAQSCRQQVAPMDDETGKELVLALYDYQEKSPREVTMKKGDILTLLNSTNKDWWKVEVNDRQGFVPAAYVKKLDPAQSASRENLLEEQGSIALRQEQIDNQTRVTKEAGSVSLRMKQVEELYHSLLELGEKRKGMLEKSCKKFMLFREANELQQWINEKEAALTSEEVGADLEQVEVLQKKFDDFQKDLKANESRLKDINKVAEDLESEGLMAEEVQAVQQQEVYGAMPRDESDSKTASPWKSARLMVHTVATFNSIKELNERWRSLQQLAEERSQLLGSAHEVQRFHRDADETKEWIEEKNQALNTDNYGHDLASVQALQRKHEGFERDLAALGDKVNSLGETAERLIQSHPESAEDLQEKCTELNQAWSSLGKRADQRKAKLGDSHDLQRFLSDFRDLMSWINGIRGLVSSDELAKDVTGAEALLERHQEHRTEIDARAGTFQAFEQFGQQLLAHGHYASPEIKEKLDVLDQERADLEKAWVQRRMMLDQCLELQLFHRDCEQAENWMAAREAFLNTEDKGDSLDSVEALIKKHEDFDKAINVQEEKIAALQSFADQLIAGGHYAKGDISSRRNEVLDRWRRLKAQMIEKRSKLGESQTLQQFSRDVDEIEAWISEKLQTASDESYKDPTNIQLSKLLSKHQKHQAFEAELHANADRIRGVIDVGNSLIDRGACAGSEDAVKARLAALADQWQFLVQKSAEKSQKLKEANKQQNFNTGIKDFDFWLSEVEALLASEDYGKDLASVNNLLKKHQLLEADISAHEDRLKDLNSQADSLMTSSAFDTSQVKDKRDTINSRFQKIKSMAASRRARLNESHRLHQFFRDMDDEESWIKEKKLLVSSEDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQFVEHWKELKQLAAARGQRLEESLEYQQFVANVEEEEAWINEKMTLVASEDYGDTLAAIQGLLKKHEAFETDFTVHKDRVNDVCTNGQDLVKKNNHHEENISSKMKGLNGKVSDLEKAASQRKAKLDENSAFLQFNWKADVVESWIGEKENSLKTDDYGRDLSSVQTLLTKQETFDAGLQAFQQEGIANITALKDQLLAAKHVQSKAIEARHAALMKRWSQLLANSATRKKKLLEAQSHFRKVEDLFLTFAKKASAFNSWFENAEEDLTDPVRCNSLEEIKALREAHDAFRSSLSSAQADFNQLAELDRQIKSFRVASNPYTWFTMEALEETWRNLQKIIKERELELQKEQRRQEENDKLRQEFAQHANAFHQWIQETRTYLLDGSCMVEESGTLESQLEATKRKHQEIRAMRSQLKKIEDLGAAMEEALILDNKYTEHSTVGLAQQWDQLDQLGMRMQHNLEQQIQARNTTGVTEEALKEFSMMFKHFDKDKSGRLNHQEFKSCLRSLGYDLPMVEEGEPDPEFEAILDTVDPNRDGHVSLQEYMAFMISRETENVKSSEEIESAFRALSSEGKPYVTKEELYQNLTREQADYCVSHMKPYVDGKGRELPTAFDYVEFTRSLFVN

Target ORF information:

RefSeq Version	XM_012147484.1
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon spectrin, alpha, non-erythrocytic 1 (SPTAN1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012147484.1

ORF Insert Sequence:

ATGCTGTCAT CGTTTCGTAA ACGCAGAGTC CAGAAAATGG ACCCAAGCGG GGTCAAAGTG 
CTGGAGACAG CCGAGGACAT CCAGGAGAGG CGGCAGCAGG TCCTGGACAG ATACCACCGC 
TTCAAGGAGC TCTCGACCCT GAGGCGCCAG AAGCTTGAAG ACTCCTACCG ATTCCAGTTT 
TTCCAAAGAG ATGCTGAGGA GCTGGAGAAA TGGATTCAGG AGAAGCTTCA GATTGCCTCG 
GATGAGAATT ACAAAGACCC AACCAACCTG CAGGGCAAGC TGCAGAAGCA TCAAGCCTTC 
GAAGCCGAGG TGCAGGCCAA CTCGGGTGCC ATCGTGAAGC TTGATGAGAC CGGTAACCTG 
ATGATCTCAG AAGGGCACTT CGCGTCCGAA ACCATCCGGA CTCGTCTGAT GGAGCTGCAC 
CGCCAGTGGG AATTGCTTCT GGAGAAGATG CGGGAGAAAG GCATCAAGCT GCTGCAGGCC 
CAGAAGCTGG TGCAGTACCT GCGGGAGTGT GAGGACGTGA TGGACTGGAT CAATGACAAG 
GAAGCCATTG TGACATCTGA GGAGCTGGGC CAGGACCTGG AGCACGTGGA GGTGTTACAG 
AAGAAATTCG AAGAGTTTCA GACAGACATG GCTGCCCACG AAGAGAGAGT CAATGAAGTG 
AACCAGTTTG CTGCCAAGCT CATTCAGGAG CAGCACCCTG AGGAGGAGCT GATCAAGACA 
AAGGAGGACG AGGTGAATGC AGCCTGGCAG CGGCTGAAGG GCCTGGCCCT CCAGCGGCAG 
GGGAAGCTGT TCGGGGCAGC TGAGGTCCAA CGCTTTAACA GGGATGTGGA CGAGACCATT 
AGCTGGATTA AGGAGAAGGA ACAGTTAATG GCCTCTGATG ACTTTGGCCG GGACCTGGCC 
AGTGTCCAGG CTCTACTGCG GAAACACGAG GGTTTGGAGA GAGATCTTGC TGCCTTGGAG 
GACAAGGTCA AAGCCCTGTG TGCCGAGGCT GACCGCCTGC AGCAGTCCCA CCCTCTGAGT 
GCCACCCAGA TCCAAGTGAA GCGAGAGGAG CTGATCACCA ACTGGGAGCA GATCCGCACC 
CTGGCCGCGG AGAGACACGC ACGTCTCAAC GATTCCTACA GGCTTCAGCG CTTCCTTGCT 
GACTTCCGAG ACCTCACCAG CTGGGTCACT GAGATGAAAG CCCTCATCAA TGCAGATGAA 
CTTGCCAATG ATGTGGCCGG GGCCGAAGCC CTGCTGGATA GGCATCAGGA GCACAAGGGT 
GAAATTGACG CTCATGAAGA TAGCTTTAAA TCTGCAGATG AGTCTGGACA GGCCCTGCTT 
GCAGCTGGTC ACTATGCCTC AGATGAAGTG AGGGAGAAGC TGACCATCCT GTCCGAGGAG 
CGGGCCGCGC TACTAGAGCT CTGGGAGCTG CGACGGCAGC AATATGAGCA GTGCATGGAC 
CTGCAGCTCT TCTACCGCGA CACGGAGCAG GTGGACAACT GGATGAGCAA GCAGGAGGCG 
TTCCTGTTGA ACGAAGACCT GGGTGATTCC TTGGATAGTG TGGAAGCGCT TCTCAAGAAG 
CACGAAGACT TTGAGAAGTC CCTCAGCGCC CAGGAGGAGA AGATCACAGC CTTAGATGAG 
TTTGCCACCA AGCTGATTCA GAACAACCAC TACGCGATGG AAGATGTGGC CACCCGGCGA 
GACGCACTGC TGAGCCGCCG CAACGCCCTC CATGAGAGAG CCATGTACCG CCGTGCCCAG 
CTTGCAGATT CCTTCCACTT GCAGCAGTTT TTCCGTGACT CTGATGAGCT CAAGAGTTGG 
GTCAACGAGA AGATGAAAAC AGCCACAGAT GAAGCTTATA AAGACCCATC CAACCTACAA 
GGGAAAGTGC AGAAGCACCA GGCCTTTGAG GCCGAGCTCT CAGCAAACCA GAGCCGCATC 
GACGCCTTGG AGAAAGCTGG GCAGAAGCTG ATCGACGTTG ACCACTATGC CAAGGATGAG 
GTGGCGACTC GGATGAATGA AGTTATCAGT CTGTGGAAGA AGCTGCTGGA GGCCACGGAG 
CTGAAAGGCA TAAAGCTCCG TGAGGCTAAC CAGCAGCAGC AGTTTAACCG CAATGTTGAG 
GACATCGAGC TGTGGCTGTA CGAAGTGGAA GGTCATCTGG CCTCGGATGA CTACGGCAAA 
GACCTCACCA ACGTCCAGAA CCTCCAGAAA AAGCATGCCC TGCTGGAGGC TGACGTGGCC 
GCTCACCAGG ATCGCATCGA TGGCATCACT ATCCAAGCCC GCCAGTTCCA GGATGCGGGC 
CACTTTGATG CTGAAAACAT CAAGAAGAAG CAGGAGGCCC TGGTAGCTCG CTACGAGGCA 
CTCAAGGAGC CCATGGTTGC CCGGAAGCAG AAGCTGGCCG ATTCCCTGCG GTTGCAGCAG 
CTCTTCCGTG ACGTTGAGGA TGAGGAGACG TGGATTCGGG AGAAAGAACC TATTGCCGCC 
TCCACCAACA GAGGCAAAGA TTTAATTGGA GTCCAGAACC TGCTAAAGAA ACACCAAGCG 
CTACAAGCAG AAATTGCTGG CCACGAGCCC CGCATCAAAG CAGTCACACA GAAGGGGAAC 
GCCATGGTAG AGGAGGGCCA TTTTGCTGCC GAGGACGTGA AGGCCAAGCT CCATGAACTG 
AACCAGAAGT GGGAGTCGCT GAAGTCCAAG GCCTCCCAGC GACGGCAGGA CCTGGAGGAC 
TCGCTGCAGG CCCAGCAGTA CTTCGCTGAT GCCAACGAGG CCGAGTCCTG GATGCGGGAG 
AAGGAGCCCA TCGTGGGCAG CACCGACTAT GGCAAAGATG AGGACTCTGC CGAGGCTCTG 
CTAAAGAAGC ACGAGGCATT GATGTCGGAT CTCAGCGCCT ATGGCAGCAG CATCCAGGCC 
CTGCGAGAGC AGGCCCAGTC GTGCCGGCAA CAAGTGGCCC CCATGGATGA CGAGACTGGG 
AAGGAGCTGG TCTTGGCGCT CTATGACTAT CAGGAGAAGA GTCCCCGAGA GGTCACCATG 
AAGAAAGGAG ACATTCTCAC CTTACTCAAC AGCACCAACA AGGACTGGTG GAAAGTGGAA 
GTGAACGACC GGCAGGGCTT CGTGCCGGCC GCCTACGTGA AGAAGCTGGA CCCAGCGCAG 
TCGGCCTCCC GGGAGAACCT CCTGGAAGAG CAGGGCAGCA TCGCGCTGCG GCAGGAGCAG 
ATTGACAACC AGACGCGCGT AACTAAGGAG GCCGGCAGCG TGTCTCTGCG TATGAAACAG 
GTGGAAGAAC TGTACCATTC TCTGCTGGAA CTGGGAGAGA AGCGTAAAGG CATGTTGGAG 
AAGAGCTGTA AGAAGTTCAT GCTGTTCCGT GAAGCGAACG AGCTGCAGCA GTGGATCAAC 
GAGAAGGAAG CGGCCCTGAC AAGCGAGGAG GTCGGCGCGG ACTTGGAGCA GGTCGAAGTG 
CTGCAGAAGA AGTTTGACGA CTTCCAGAAG GATCTCAAGG CCAATGAGTC ACGGCTGAAG 
GACATTAACA AGGTGGCTGA AGACCTGGAG TCGGAAGGCC TGATGGCGGA GGAGGTGCAG 
GCCGTGCAGC AGCAGGAGGT GTATGGCGCG ATGCCCAGGG ATGAATCTGA TTCCAAGACA 
GCCTCCCCGT GGAAGTCTGC TCGCCTGATG GTTCACACCG TGGCCACCTT TAATTCCATC 
AAGGAGCTCA ATGAGCGCTG GCGGTCCCTG CAGCAGCTGG CTGAGGAGCG GAGCCAACTC 
TTGGGCAGCG CCCACGAAGT GCAGCGGTTC CACAGAGATG CTGACGAAAC CAAAGAGTGG 
ATCGAGGAGA AGAACCAGGC TCTAAACACA GACAACTATG GCCACGACCT GGCCAGCGTC 
CAGGCCCTGC AGCGCAAGCA TGAGGGCTTC GAGAGGGACC TCGCGGCCCT TGGCGACAAG 
GTGAACTCGC TCGGGGAGAC AGCCGAGCGC CTGATCCAGT CCCACCCCGA GTCGGCAGAG 
GACCTGCAGG AGAAGTGCAC TGAGCTGAAC CAGGCATGGA GCAGCCTGGG CAAGCGTGCC 
GACCAGCGCA AGGCCAAGCT GGGCGACTCC CACGACCTGC AGCGCTTCCT CAGCGACTTC 
CGGGACCTCA TGTCTTGGAT CAATGGAATC CGAGGTCTGG TGTCCTCAGA CGAGCTAGCC 
AAGGATGTCA CTGGAGCTGA GGCCCTGCTG GAGCGACACC AGGAACACCG GACAGAAATT 
GATGCCAGAG CTGGCACTTT CCAGGCATTC GAGCAGTTTG GGCAGCAGCT GTTGGCTCAT 
GGGCACTACG CCAGCCCCGA GATCAAGGAG AAGCTCGATG TTCTAGACCA GGAGCGCGCA 
GACCTCGAGA AGGCCTGGGT CCAGCGCAGG ATGATGCTGG ATCAGTGCCT TGAATTGCAG 
CTGTTCCATC GGGATTGCGA GCAGGCCGAG AACTGGATGG CTGCCCGGGA GGCCTTCCTG 
AACACAGAAG ACAAAGGGGA CTCGCTAGAC AGCGTGGAGG CTCTGATCAA AAAACACGAG 
GACTTTGACA AAGCAATTAA TGTCCAGGAG GAGAAGATTG CTGCCCTGCA GTCCTTTGCT 
GACCAGCTCA TTGCTGGCGG TCACTACGCC AAAGGCGACA TCTCCAGCCG GCGCAATGAG 
GTTCTGGACA GGTGGCGCCG TCTAAAAGCT CAGATGATTG AGAAGAGGTC AAAGCTGGGA 
GAATCGCAGA CCCTCCAGCA GTTCAGCCGG GATGTAGACG AGATTGAAGC TTGGATCAGT 
GAAAAATTAC AGACTGCAAG TGATGAGTCA TATAAGGACC CCACCAACAT CCAGCTTTCC 
AAGCTGCTGA GCAAGCATCA GAAGCACCAG GCCTTCGAGG CAGAACTGCA CGCCAATGCG 
GACCGGATCC GCGGGGTCAT CGACGTGGGC AACTCCCTCA TTGACCGTGG GGCCTGTGCC 
GGCAGCGAGG ATGCCGTCAA GGCCCGGCTG GCCGCCTTAG CTGACCAGTG GCAGTTCCTG 
GTGCAGAAGT CGGCTGAGAA GAGCCAGAAG CTGAAAGAAG CCAACAAACA GCAGAACTTC 
AACACTGGCA TCAAGGACTT CGACTTCTGG CTCTCTGAGG TGGAAGCCCT GCTGGCCTCT 
GAGGATTATG GCAAAGACCT GGCTTCTGTG AACAACTTGC TAAAAAAACA TCAGCTGCTG 
GAGGCAGATA TCTCTGCCCA CGAGGACCGC CTGAAGGACC TCAACAGCCA GGCGGACAGC 
CTGATGACCA GCAGCGCCTT CGACACCTCC CAGGTGAAGG ATAAGCGGGA CACCATCAAC 
AGCCGTTTCC AGAAGATCAA GAGCATGGCC GCCTCCCGGC GAGCTAGGCT GAATGAGTCC 
CACCGCCTGC ACCAGTTCTT CCGGGACATG GACGATGAGG AGTCCTGGAT CAAGGAGAAG 
AAGCTGCTGG TGAGCTCAGA GGACTACGGC CGCGACCTGA CGGGCGTCCA GAACCTGAGG 
AAGAAGCACA AACGGCTGGA GGCAGAGCTG GCTGCCCACG AGCCGGCCAT TCAGGGTGTC 
CTGGACACTG GAAAGAAGCT GTCTGATGAC AACACCATCG GGAAGGAGGA GATTCAGCAG 
CGCCTGGCGC AGTTTGTGGA GCACTGGAAG GAGCTGAAGC AGCTGGCAGC TGCTCGGGGC 
CAGCGGCTAG AAGAATCTTT GGAATATCAG CAGTTTGTGG CCAACGTGGA GGAGGAGGAA 
GCCTGGATCA ATGAGAAGAT GACCCTGGTG GCCAGTGAGG ATTACGGAGA CACTCTTGCT 
GCCATCCAGG GCTTGCTGAA GAAACACGAA GCCTTCGAGA CGGACTTCAC TGTCCACAAG 
GACCGGGTGA ATGACGTCTG CACTAACGGA CAAGACCTGG TTAAGAAGAA CAATCACCAT 
GAGGAGAACA TCTCTTCCAA GATGAAGGGC TTGAACGGGA AAGTGTCAGA CTTGGAGAAG 
GCTGCCTCCC AGAGGAAGGC AAAGCTCGAC GAGAACTCGG CCTTCCTCCA GTTCAACTGG 
AAGGCCGATG TGGTGGAGTC CTGGATTGGT GAAAAGGAGA ACAGCTTGAA GACAGATGAT 
TATGGTCGAG ATCTGTCTTC TGTCCAAACT CTCCTCACCA AACAGGAAAC CTTCGATGCT 
GGCCTGCAGG CCTTCCAGCA GGAGGGCATC GCCAACATCA CTGCCCTCAA AGATCAGCTG 
CTGGCCGCCA AGCATGTTCA GTCCAAGGCC ATCGAGGCCC GCCACGCTGC CCTCATGAAG 
AGATGGAGCC AGCTCCTGGC CAATTCGGCC ACCCGGAAGA AGAAGCTGCT GGAGGCACAG 
AGTCACTTCC GCAAGGTGGA GGATCTCTTC CTGACCTTTG CCAAAAAGGC TTCAGCCTTC 
AACAGCTGGT TTGAAAATGC AGAGGAGGAC TTAACAGACC CAGTGCGCTG CAACTCCCTG 
GAAGAGATCA AGGCCTTGAG AGAGGCCCAC GATGCCTTCC GCTCCTCGCT GAGCTCAGCC 
CAGGCCGACT TCAACCAGCT GGCTGAGCTG GACCGCCAGA TCAAGAGCTT CCGCGTGGCC 
TCCAACCCCT ACACCTGGTT CACCATGGAG GCCCTGGAGG AGACCTGGAG GAACCTTCAG 
AAGATCATCA AGGAAAGGGA GCTGGAGCTG CAGAAGGAGC AGCGGCGGCA GGAGGAGAAC 
GACAAGCTGC GCCAGGAGTT CGCCCAGCAT GCCAACGCCT TCCACCAGTG GATCCAGGAG 
ACCAGAACGT ATCTCCTCGA TGGGTCCTGC ATGGTGGAAG AATCTGGGAC CCTTGAATCG 
CAGCTTGAAG CTACCAAACG CAAACACCAG GAGATTCGAG CCATGAGAAG TCAGCTCAAG 
AAGATTGAAG ACCTGGGGGC GGCCATGGAG GAAGCGCTGA TCCTGGACAA CAAGTACACG 
GAGCACAGCA CCGTGGGCCT GGCCCAGCAG TGGGACCAGC TCGACCAGCT GGGCATGCGG 
ATGCAGCACA ACCTGGAGCA GCAGATCCAG GCCAGGAACA CGACAGGAGT GACCGAGGAG 
GCCCTCAAAG AATTCAGCAT GATGTTCAAA CACTTCGACA AGGACAAGTC CGGCCGGCTG 
AATCACCAGG AGTTCAAATC CTGTCTGCGC TCCCTGGGCT ATGACCTGCC CATGGTGGAG 
GAAGGGGAGC CCGACCCTGA GTTCGAGGCC ATCTTGGATA CTGTGGATCC TAACAGGGAC 
GGCCACGTCT CCCTGCAAGA GTACATGGCC TTTATGATCA GCCGTGAGAC CGAGAATGTC 
AAGTCCAGCG AGGAGATTGA GAGCGCCTTC CGGGCCCTGA GCTCCGAAGG GAAGCCTTAT 
GTGACCAAGG AGGAGCTGTA CCAGAACCTG ACTCGGGAAC AAGCCGACTA CTGCGTCTCC 
CACATGAAGC CCTACGTGGA CGGCAAGGGC CGCGAACTCC CCACTGCTTT CGACTACGTT 
GAGTTCACCC GCTCGCTCTT CGTGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTAN1 ( XM_012147485.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_012002875.1 Ovis aries musimon spectrin, alpha, non-erythrocytic 1 (SPTAN1), transcript variant X9, mRNA.

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CloneID	OOv24455
Clone ID Related Accession (Same CDS sequence)	XM_012147485.1
Accession Version	XM_012147485.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7455bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	spectrin alpha chain, non-erythrocytic 1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942583.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTCAT CGTTTCGTAA ACGCAGAGTC CAGAAAATGG ACCCAAGCGG GGTCAAAGTG 
CTGGAGACAG CCGAGGACAT CCAGGAGAGG CGGCAGCAGG TCCTGGACAG ATACCACCGC 
TTCAAGGAGC TCTCGACCCT GAGGCGCCAG AAGCTTGAAG ACTCCTACCG ATTCCAGTTT 
TTCCAAAGAG ATGCTGAGGA GCTGGAGAAA TGGATTCAGG AGAAGCTTCA GATTGCCTCG 
GATGAGAATT ACAAAGACCC AACCAACCTG CAGGGCAAGC TGCAGAAGCA TCAAGCCTTC 
GAAGCCGAGG TGCAGGCCAA CTCGGGTGCC ATCGTGAAGC TTGATGAGAC CGGTAACCTG 
ATGATCTCAG AAGGGCACTT CGCGTCCGAA ACCATCCGGA CTCGTCTGAT GGAGCTGCAC 
CGCCAGTGGG AATTGCTTCT GGAGAAGATG CGGGAGAAAG GCATCAAGCT GCTGCAGGCC 
CAGAAGCTGG TGCAGTACCT GCGGGAGTGT GAGGACGTGA TGGACTGGAT CAATGACAAG 
GAAGCCATTG TGACATCTGA GGAGCTGGGC CAGGACCTGG AGCACGTGGA GGTGTTACAG 
AAGAAATTCG AAGAGTTTCA GACAGACATG GCTGCCCACG AAGAGAGAGT CAATGAAGTG 
AACCAGTTTG CTGCCAAGCT CATTCAGGAG CAGCACCCTG AGGAGGAGCT GATCAAGACA 
AAGGAGGACG AGGTGAATGC AGCCTGGCAG CGGCTGAAGG GCCTGGCCCT CCAGCGGCAG 
GGGAAGCTGT TCGGGGCAGC TGAGGTCCAA CGCTTTAACA GGGATGTGGA CGAGACCATT 
AGCTGGATTA AGGAGAAGGA ACAGTTAATG GCCTCTGATG ACTTTGGCCG GGACCTGGCC 
AGTGTCCAGG CTCTACTGCG GAAACACGAG GGTTTGGAGA GAGATCTTGC TGCCTTGGAG 
GACAAGGTCA AAGCCCTGTG TGCCGAGGCT GACCGCCTGC AGCAGTCCCA CCCTCTGAGT 
GCCACCCAGA TCCAAGTGAA GCGAGAGGAG CTGATCACCA ACTGGGAGCA GATCCGCACC 
CTGGCCGCGG AGAGACACGC ACGTCTCAAC GATTCCTACA GGCTTCAGCG CTTCCTTGCT 
GACTTCCGAG ACCTCACCAG CTGGGTCACT GAGATGAAAG CCCTCATCAA TGCAGATGAA 
CTTGCCAATG ATGTGGCCGG GGCCGAAGCC CTGCTGGATA GGCATCAGGA GCACAAGGGT 
GAAATTGACG CTCATGAAGA TAGCTTTAAA TCTGCAGATG AGTCTGGACA GGCCCTGCTT 
GCAGCTGGTC ACTATGCCTC AGATGAAGTG AGGGAGAAGC TGACCATCCT GTCCGAGGAG 
CGGGCCGCGC TACTAGAGCT CTGGGAGCTG CGACGGCAGC AATATGAGCA GTGCATGGAC 
CTGCAGCTCT TCTACCGCGA CACGGAGCAG GTGGACAACT GGATGAGCAA GCAGGAGGCG 
TTCCTGTTGA ACGAAGACCT GGGTGATTCC TTGGATAGTG TGGAAGCGCT TCTCAAGAAG 
CACGAAGACT TTGAGAAGTC CCTCAGCGCC CAGGAGGAGA AGATCACAGC CTTAGATGAG 
TTTGCCACCA AGCTGATTCA GAACAACCAC TACGCGATGG AAGATGTGGC CACCCGGCGA 
GACGCACTGC TGAGCCGCCG CAACGCCCTC CATGAGAGAG CCATGTACCG CCGTGCCCAG 
CTTGCAGATT CCTTCCACTT GCAGCAGTTT TTCCGTGACT CTGATGAGCT CAAGAGTTGG 
GTCAACGAGA AGATGAAAAC AGCCACAGAT GAAGCTTATA AAGACCCATC CAACCTACAA 
GGGAAAGTGC AGAAGCACCA GGCCTTTGAG GCCGAGCTCT CAGCAAACCA GAGCCGCATC 
GACGCCTTGG AGAAAGCTGG GCAGAAGCTG ATCGACGTTG ACCACTATGC CAAGGATGAG 
GTGGCGACTC GGATGAATGA AGTTATCAGT CTGTGGAAGA AGCTGCTGGA GGCCACGGAG 
CTGAAAGGCA TAAAGCTCCG TGAGGCTAAC CAGCAGCAGC AGTTTAACCG CAATGTTGAG 
GACATCGAGC TGTGGCTGTA CGAAGTGGAA GGTCATCTGG CCTCGGATGA CTACGGCAAA 
GACCTCACCA ACGTCCAGAA CCTCCAGAAA AAGCATGCCC TGCTGGAGGC TGACGTGGCC 
GCTCACCAGG ATCGCATCGA TGGCATCACT ATCCAAGCCC GCCAGTTCCA GGATGCGGGC 
CACTTTGATG CTGAAAACAT CAAGAAGAAG CAGGAGGCCC TGGTAGCTCG CTACGAGGCA 
CTCAAGGAGC CCATGGTTGC CCGGAAGCAG AAGCTGGCCG ATTCCCTGCG GTTGCAGCAG 
CTCTTCCGTG ACGTTGAGGA TGAGGAGACG TGGATTCGGG AGAAAGAACC TATTGCCGCC 
TCCACCAACA GAGGCAAAGA TTTAATTGGA GTCCAGAACC TGCTAAAGAA ACACCAAGCG 
CTACAAGCAG AAATTGCTGG CCACGAGCCC CGCATCAAAG CAGTCACACA GAAGGGGAAC 
GCCATGGTAG AGGAGGGCCA TTTTGCTGCC GAGGACGTGA AGGCCAAGCT CCATGAACTG 
AACCAGAAGT GGGAGTCGCT GAAGTCCAAG GCCTCCCAGC GACGGCAGGA CCTGGAGGAC 
TCGCTGCAGG CCCAGCAGTA CTTCGCTGAT GCCAACGAGG CCGAGTCCTG GATGCGGGAG 
AAGGAGCCCA TCGTGGGCAG CACCGACTAT GGCAAAGATG AGGACTCTGC CGAGGCTCTG 
CTAAAGAAGC ACGAGGCATT GATGTCGGAT CTCAGCGCCT ATGGCAGCAG CATCCAGGCC 
CTGCGAGAGC AGGCCCAGTC GTGCCGGCAA CAAGTGGCCC CCATGGATGA CGAGACTGGG 
AAGGAGCTGG TCTTGGCGCT CTATGACTAT CAGGAGAAGA GTCCCCGAGA GGTCACCATG 
AAGAAAGGAG ACATTCTCAC CTTACTCAAC AGCACCAACA AGGACTGGTG GAAAGTGGAA 
GTGAACGACC GGCAGGGCTT CGTGCCGGCC GCCTACGTGA AGAAGCTGGA CCCAGCGCAG 
TCGGCCTCCC GGGAGAACCT CCTGGAAGAG CAGGGCAGCA TCGCGCTGCG GCAGGAGCAG 
ATTGACAACC AGACGCGCGT AACTAAGGAG GCCGGCAGCG TGTCTCTGCG TATGAAACAG 
GTGGAAGAAC TGTACCATTC TCTGCTGGAA CTGGGAGAGA AGCGTAAAGG CATGTTGGAG 
AAGAGCTGTA AGAAGTTCAT GCTGTTCCGT GAAGCGAACG AGCTGCAGCA GTGGATCAAC 
GAGAAGGAAG CGGCCCTGAC AAGCGAGGAG GTCGGCGCGG ACTTGGAGCA GGTCGAAGTG 
CTGCAGAAGA AGTTTGACGA CTTCCAGAAG GATCTCAAGG CCAATGAGTC ACGGCTGAAG 
GACATTAACA AGGTGGCTGA AGACCTGGAG TCGGAAGGCC TGATGGCGGA GGAGGTGCAG 
GCCGTGCAGC AGCAGGAGGT GTATGGCGCG ATGCCCAGGG ATGAATCTGA TTCCAAGACA 
GCCTCCCCGT GGAAGTCTGC TCGCCTGATG GTTCACACCG TGGCCACCTT TAATTCCATC 
AAGGAGCTCA ATGAGCGCTG GCGGTCCCTG CAGCAGCTGG CTGAGGAGCG GAGCCAACTC 
TTGGGCAGCG CCCACGAAGT GCAGCGGTTC CACAGAGATG CTGACGAAAC CAAAGAGTGG 
ATCGAGGAGA AGAACCAGGC TCTAAACACA GACAACTATG GCCACGACCT GGCCAGCGTC 
CAGGCCCTGC AGCGCAAGCA TGAGGGCTTC GAGAGGGACC TCGCGGCCCT TGGCGACAAG 
GTGAACTCGC TCGGGGAGAC AGCCGAGCGC CTGATCCAGT CCCACCCCGA GTCGGCAGAG 
GACCTGCAGG AGAAGTGCAC TGAGCTGAAC CAGGCATGGA GCAGCCTGGG CAAGCGTGCC 
GACCAGCGCA AGGCCAAGCT GGGCGACTCC CACGACCTGC AGCGCTTCCT CAGCGACTTC 
CGGGACCTCA TGTCTTGGAT CAATGGAATC CGAGGTCTGG TGTCCTCAGA CGAGCTAGCC 
AAGGATGTCA CTGGAGCTGA GGCCCTGCTG GAGCGACACC AGGAACACCG GACAGAAATT 
GATGCCAGAG CTGGCACTTT CCAGGCATTC GAGCAGTTTG GGCAGCAGCT GTTGGCTCAT 
GGGCACTACG CCAGCCCCGA GATCAAGGAG AAGCTCGATG TTCTAGACCA GGAGCGCGCA 
GACCTCGAGA AGGCCTGGGT CCAGCGCAGG ATGATGCTGG ATCAGTGCCT TGAATTGCAG 
CTGTTCCATC GGGATTGCGA GCAGGCCGAG AACTGGATGG CTGCCCGGGA GGCCTTCCTG 
AACACAGAAG ACAAAGGGGA CTCGCTAGAC AGCGTGGAGG CTCTGATCAA AAAACACGAG 
GACTTTGACA AAGCAATTAA TGTCCAGGAG GAGAAGATTG CTGCCCTGCA GTCCTTTGCT 
GACCAGCTCA TTGCTGGCGG TCACTACGCC AAAGGCGACA TCTCCAGCCG GCGCAATGAG 
GTTCTGGACA GGTGGCGCCG TCTAAAAGCT CAGATGATTG AGAAGAGGTC AAAGCTGGGA 
GAATCGCAGA CCCTCCAGCA GTTCAGCCGG GATGTAGACG AGATTGAAGC TTGGATCAGT 
GAAAAATTAC AGACTGCAAG TGATGAGTCA TATAAGGACC CCACCAACAT CCAGAGCAAG 
CATCAGAAGC ACCAGGCCTT CGAGGCAGAA CTGCACGCCA ATGCGGACCG GATCCGCGGG 
GTCATCGACG TGGGCAACTC CCTCATTGAC CGTGGGGCCT GTGCCGGCAG CGAGGATGCC 
GTCAAGGCCC GGCTGGCCGC CTTAGCTGAC CAGTGGCAGT TCCTGGTGCA GAAGTCGGCT 
GAGAAGAGCC AGAAGCTGAA AGAAGCCAAC AAACAGCAGA ACTTCAACAC TGGCATCAAG 
GACTTCGACT TCTGGCTCTC TGAGGTGGAA GCCCTGCTGG CCTCTGAGGA TTATGGCAAA 
GACCTGGCTT CTGTGAACAA CTTGCTAAAA AAACATCAGC TGCTGGAGGC AGATATCTCT 
GCCCACGAGG ACCGCCTGAA GGACCTCAAC AGCCAGGCGG ACAGCCTGAT GACCAGCAGC 
GCCTTCGACA CCTCCCAGGT GAAGGATAAG CGGGACACCA TCAACAGCCG TTTCCAGAAG 
ATCAAGAGCA TGGCCGCCTC CCGGCGAGCT AGGCTGAATG AGTCCCACCG CCTGCACCAG 
TTCTTCCGGG ACATGGACGA TGAGGAGTCC TGGATCAAGG AGAAGAAGCT GCTGGTGAGC 
TCAGAGGACT ACGGCCGCGA CCTGACGGGC GTCCAGAACC TGAGGAAGAA GCACAAACGG 
CTGGAGGCAG AGCTGGCTGC CCACGAGCCG GCCATTCAGG GTGTCCTGGA CACTGGAAAG 
AAGCTGTCTG ATGACAACAC CATCGGGAAG GAGGAGATTC AGCAGCGCCT GGCGCAGTTT 
GTGGAGCACT GGAAGGAGCT GAAGCAGCTG GCAGCTGCTC GGGGCCAGCG GCTAGAAGAA 
TCTTTGGAAT ATCAGCAGTT TGTGGCCAAC GTGGAGGAGG AGGAAGCCTG GATCAATGAG 
AAGATGACCC TGGTGGCCAG TGAGGATTAC GGAGACACTC TTGCTGCCAT CCAGGGCTTG 
CTGAAGAAAC ACGAAGCCTT CGAGACGGAC TTCACTGTCC ACAAGGACCG GGTGAATGAC 
GTCTGCACTA ACGGACAAGA CCTGGTTAAG AAGAACAATC ACCATGAGGA GAACATCTCT 
TCCAAGATGA AGGGCTTGAA CGGGAAAGTG TCAGACTTGG AGAAGGCTGC CTCCCAGAGG 
AAGGCAAAGC TCGACGAGAA CTCGGCCTTC CTCCAGTTCA ACTGGAAGGC CGATGTGGTG 
GAGTCCTGGA TTGGTGAAAA GGAGAACAGC TTGAAGACAG ATGATTATGG TCGAGATCTG 
TCTTCTGTCC AAACTCTCCT CACCAAACAG GAAACCTTCG ATGCTGGCCT GCAGGCCTTC 
CAGCAGGAGG GCATCGCCAA CATCACTGCC CTCAAAGATC AGCTGCTGGC CGCCAAGCAT 
GTTCAGTCCA AGGCCATCGA GGCCCGCCAC GCTGCCCTCA TGAAGAGATG GAGCCAGCTC 
CTGGCCAATT CGGCCACCCG GAAGAAGAAG CTGCTGGAGG CACAGAGTCA CTTCCGCAAG 
GTGGAGGATC TCTTCCTGAC CTTTGCCAAA AAGGCTTCAG CCTTCAACAG CTGGTTTGAA 
AATGCAGAGG AGGACTTAAC AGACCCAGTG CGCTGCAACT CCCTGGAAGA GATCAAGGCC 
TTGAGAGAGG CCCACGATGC CTTCCGCTCC TCGCTGAGCT CAGCCCAGGC CGACTTCAAC 
CAGCTGGCTG AGCTGGACCG CCAGATCAAG AGCTTCCGCG TGGCCTCCAA CCCCTACACC 
TGGTTCACCA TGGAGGCCCT GGAGGAGACC TGGAGGAACC TTCAGAAGAT CATCAAGGAA 
AGGGAGCTGG AGCTGCAGAA GGAGCAGCGG CGGCAGGAGG AGAACGACAA GCTGCGCCAG 
GAGTTCGCCC AGCATGCCAA CGCCTTCCAC CAGTGGATCC AGGAGACCAG AACGTATCTC 
CTCGATGGGT CCTGCATGGT GGAAGAATCT GGGACCCTTG AATCGCAGCT TGAAGCTACC 
AAACGCAAAC ACCAGGAGAT TCGAGCCATG AGAAGTCAGC TCAAGAAGAT TGAAGACCTG 
GGGGCGGCCA TGGAGGAAGC GCTGATCCTG GACAACAAGT ACACGGAGCA CAGCACCGTG 
GGCCTGGCCC AGCAGTGGGA CCAGCTCGAC CAGCTGGGCA TGCGGATGCA GCACAACCTG 
GAGCAGCAGA TCCAGGCCAG GAACACGACA GGAGTGACCG AGGAGGCCCT CAAAGAATTC 
AGCATGATGT TCAAACACTT CGACAAGGAC AAGTCCGGCC GGCTGAATCA CCAGGAGTTC 
AAATCCTGTC TGCGCTCCCT GGGCTATGAC CTGCCCATGG TGGAGGAAGG GGAGCCCGAC 
CCTGAGTTCG AGGCCATCTT GGATACTGTG GATCCTAACA GGGACGGCCA CGTCTCCCTG 
CAAGAGTACA TGGCCTTTAT GATCAGCCGT GAGACCGAGA ATGTCAAGTC CAGCGAGGAG 
ATTGAGAGCG CCTTCCGGGC CCTGAGCTCC GAAGGGAAGC CTTATGTGAC CAAGGAGGAG 
CTGTACCAGA ACCTGACTCG GGAACAAGCC GACTACTGCG TCTCCCACAT GAAGCCCTAC 
GTGGACGGCA AGGGCCGCGA ACTCCCCACT GCTTTCGACT ACGTTGAGTT CACCCGCTCG 
CTCTTCGTGA ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012002875.1
CDS	81..7535
Translation	MLSSFRKRRVQKMDPSGVKVLETAEDIQERRQQVLDRYHRFKELSTLRRQKLEDSYRFQFFQRDAEELEKWIQEKLQIASDENYKDPTNLQGKLQKHQAFEAEVQANSGAIVKLDETGNLMISEGHFASETIRTRLMELHRQWELLLEKMREKGIKLLQAQKLVQYLRECEDVMDWINDKEAIVTSEELGQDLEHVEVLQKKFEEFQTDMAAHEERVNEVNQFAAKLIQEQHPEEELIKTKEDEVNAAWQRLKGLALQRQGKLFGAAEVQRFNRDVDETISWIKEKEQLMASDDFGRDLASVQALLRKHEGLERDLAALEDKVKALCAEADRLQQSHPLSATQIQVKREELITNWEQIRTLAAERHARLNDSYRLQRFLADFRDLTSWVTEMKALINADELANDVAGAEALLDRHQEHKGEIDAHEDSFKSADESGQALLAAGHYASDEVREKLTILSEERAALLELWELRRQQYEQCMDLQLFYRDTEQVDNWMSKQEAFLLNEDLGDSLDSVEALLKKHEDFEKSLSAQEEKITALDEFATKLIQNNHYAMEDVATRRDALLSRRNALHERAMYRRAQLADSFHLQQFFRDSDELKSWVNEKMKTATDEAYKDPSNLQGKVQKHQAFEAELSANQSRIDALEKAGQKLIDVDHYAKDEVATRMNEVISLWKKLLEATELKGIKLREANQQQQFNRNVEDIELWLYEVEGHLASDDYGKDLTNVQNLQKKHALLEADVAAHQDRIDGITIQARQFQDAGHFDAENIKKKQEALVARYEALKEPMVARKQKLADSLRLQQLFRDVEDEETWIREKEPIAASTNRGKDLIGVQNLLKKHQALQAEIAGHEPRIKAVTQKGNAMVEEGHFAAEDVKAKLHELNQKWESLKSKASQRRQDLEDSLQAQQYFADANEAESWMREKEPIVGSTDYGKDEDSAEALLKKHEALMSDLSAYGSSIQALREQAQSCRQQVAPMDDETGKELVLALYDYQEKSPREVTMKKGDILTLLNSTNKDWWKVEVNDRQGFVPAAYVKKLDPAQSASRENLLEEQGSIALRQEQIDNQTRVTKEAGSVSLRMKQVEELYHSLLELGEKRKGMLEKSCKKFMLFREANELQQWINEKEAALTSEEVGADLEQVEVLQKKFDDFQKDLKANESRLKDINKVAEDLESEGLMAEEVQAVQQQEVYGAMPRDESDSKTASPWKSARLMVHTVATFNSIKELNERWRSLQQLAEERSQLLGSAHEVQRFHRDADETKEWIEEKNQALNTDNYGHDLASVQALQRKHEGFERDLAALGDKVNSLGETAERLIQSHPESAEDLQEKCTELNQAWSSLGKRADQRKAKLGDSHDLQRFLSDFRDLMSWINGIRGLVSSDELAKDVTGAEALLERHQEHRTEIDARAGTFQAFEQFGQQLLAHGHYASPEIKEKLDVLDQERADLEKAWVQRRMMLDQCLELQLFHRDCEQAENWMAAREAFLNTEDKGDSLDSVEALIKKHEDFDKAINVQEEKIAALQSFADQLIAGGHYAKGDISSRRNEVLDRWRRLKAQMIEKRSKLGESQTLQQFSRDVDEIEAWISEKLQTASDESYKDPTNIQSKHQKHQAFEAELHANADRIRGVIDVGNSLIDRGACAGSEDAVKARLAALADQWQFLVQKSAEKSQKLKEANKQQNFNTGIKDFDFWLSEVEALLASEDYGKDLASVNNLLKKHQLLEADISAHEDRLKDLNSQADSLMTSSAFDTSQVKDKRDTINSRFQKIKSMAASRRARLNESHRLHQFFRDMDDEESWIKEKKLLVSSEDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQFVEHWKELKQLAAARGQRLEESLEYQQFVANVEEEEAWINEKMTLVASEDYGDTLAAIQGLLKKHEAFETDFTVHKDRVNDVCTNGQDLVKKNNHHEENISSKMKGLNGKVSDLEKAASQRKAKLDENSAFLQFNWKADVVESWIGEKENSLKTDDYGRDLSSVQTLLTKQETFDAGLQAFQQEGIANITALKDQLLAAKHVQSKAIEARHAALMKRWSQLLANSATRKKKLLEAQSHFRKVEDLFLTFAKKASAFNSWFENAEEDLTDPVRCNSLEEIKALREAHDAFRSSLSSAQADFNQLAELDRQIKSFRVASNPYTWFTMEALEETWRNLQKIIKERELELQKEQRRQEENDKLRQEFAQHANAFHQWIQETRTYLLDGSCMVEESGTLESQLEATKRKHQEIRAMRSQLKKIEDLGAAMEEALILDNKYTEHSTVGLAQQWDQLDQLGMRMQHNLEQQIQARNTTGVTEEALKEFSMMFKHFDKDKSGRLNHQEFKSCLRSLGYDLPMVEEGEPDPEFEAILDTVDPNRDGHVSLQEYMAFMISRETENVKSSEEIESAFRALSSEGKPYVTKEELYQNLTREQADYCVSHMKPYVDGKGRELPTAFDYVEFTRSLFVN

Target ORF information:

RefSeq Version	XM_012147485.1
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon spectrin, alpha, non-erythrocytic 1 (SPTAN1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012147485.1

ORF Insert Sequence:

ATGCTGTCAT CGTTTCGTAA ACGCAGAGTC CAGAAAATGG ACCCAAGCGG GGTCAAAGTG 
CTGGAGACAG CCGAGGACAT CCAGGAGAGG CGGCAGCAGG TCCTGGACAG ATACCACCGC 
TTCAAGGAGC TCTCGACCCT GAGGCGCCAG AAGCTTGAAG ACTCCTACCG ATTCCAGTTT 
TTCCAAAGAG ATGCTGAGGA GCTGGAGAAA TGGATTCAGG AGAAGCTTCA GATTGCCTCG 
GATGAGAATT ACAAAGACCC AACCAACCTG CAGGGCAAGC TGCAGAAGCA TCAAGCCTTC 
GAAGCCGAGG TGCAGGCCAA CTCGGGTGCC ATCGTGAAGC TTGATGAGAC CGGTAACCTG 
ATGATCTCAG AAGGGCACTT CGCGTCCGAA ACCATCCGGA CTCGTCTGAT GGAGCTGCAC 
CGCCAGTGGG AATTGCTTCT GGAGAAGATG CGGGAGAAAG GCATCAAGCT GCTGCAGGCC 
CAGAAGCTGG TGCAGTACCT GCGGGAGTGT GAGGACGTGA TGGACTGGAT CAATGACAAG 
GAAGCCATTG TGACATCTGA GGAGCTGGGC CAGGACCTGG AGCACGTGGA GGTGTTACAG 
AAGAAATTCG AAGAGTTTCA GACAGACATG GCTGCCCACG AAGAGAGAGT CAATGAAGTG 
AACCAGTTTG CTGCCAAGCT CATTCAGGAG CAGCACCCTG AGGAGGAGCT GATCAAGACA 
AAGGAGGACG AGGTGAATGC AGCCTGGCAG CGGCTGAAGG GCCTGGCCCT CCAGCGGCAG 
GGGAAGCTGT TCGGGGCAGC TGAGGTCCAA CGCTTTAACA GGGATGTGGA CGAGACCATT 
AGCTGGATTA AGGAGAAGGA ACAGTTAATG GCCTCTGATG ACTTTGGCCG GGACCTGGCC 
AGTGTCCAGG CTCTACTGCG GAAACACGAG GGTTTGGAGA GAGATCTTGC TGCCTTGGAG 
GACAAGGTCA AAGCCCTGTG TGCCGAGGCT GACCGCCTGC AGCAGTCCCA CCCTCTGAGT 
GCCACCCAGA TCCAAGTGAA GCGAGAGGAG CTGATCACCA ACTGGGAGCA GATCCGCACC 
CTGGCCGCGG AGAGACACGC ACGTCTCAAC GATTCCTACA GGCTTCAGCG CTTCCTTGCT 
GACTTCCGAG ACCTCACCAG CTGGGTCACT GAGATGAAAG CCCTCATCAA TGCAGATGAA 
CTTGCCAATG ATGTGGCCGG GGCCGAAGCC CTGCTGGATA GGCATCAGGA GCACAAGGGT 
GAAATTGACG CTCATGAAGA TAGCTTTAAA TCTGCAGATG AGTCTGGACA GGCCCTGCTT 
GCAGCTGGTC ACTATGCCTC AGATGAAGTG AGGGAGAAGC TGACCATCCT GTCCGAGGAG 
CGGGCCGCGC TACTAGAGCT CTGGGAGCTG CGACGGCAGC AATATGAGCA GTGCATGGAC 
CTGCAGCTCT TCTACCGCGA CACGGAGCAG GTGGACAACT GGATGAGCAA GCAGGAGGCG 
TTCCTGTTGA ACGAAGACCT GGGTGATTCC TTGGATAGTG TGGAAGCGCT TCTCAAGAAG 
CACGAAGACT TTGAGAAGTC CCTCAGCGCC CAGGAGGAGA AGATCACAGC CTTAGATGAG 
TTTGCCACCA AGCTGATTCA GAACAACCAC TACGCGATGG AAGATGTGGC CACCCGGCGA 
GACGCACTGC TGAGCCGCCG CAACGCCCTC CATGAGAGAG CCATGTACCG CCGTGCCCAG 
CTTGCAGATT CCTTCCACTT GCAGCAGTTT TTCCGTGACT CTGATGAGCT CAAGAGTTGG 
GTCAACGAGA AGATGAAAAC AGCCACAGAT GAAGCTTATA AAGACCCATC CAACCTACAA 
GGGAAAGTGC AGAAGCACCA GGCCTTTGAG GCCGAGCTCT CAGCAAACCA GAGCCGCATC 
GACGCCTTGG AGAAAGCTGG GCAGAAGCTG ATCGACGTTG ACCACTATGC CAAGGATGAG 
GTGGCGACTC GGATGAATGA AGTTATCAGT CTGTGGAAGA AGCTGCTGGA GGCCACGGAG 
CTGAAAGGCA TAAAGCTCCG TGAGGCTAAC CAGCAGCAGC AGTTTAACCG CAATGTTGAG 
GACATCGAGC TGTGGCTGTA CGAAGTGGAA GGTCATCTGG CCTCGGATGA CTACGGCAAA 
GACCTCACCA ACGTCCAGAA CCTCCAGAAA AAGCATGCCC TGCTGGAGGC TGACGTGGCC 
GCTCACCAGG ATCGCATCGA TGGCATCACT ATCCAAGCCC GCCAGTTCCA GGATGCGGGC 
CACTTTGATG CTGAAAACAT CAAGAAGAAG CAGGAGGCCC TGGTAGCTCG CTACGAGGCA 
CTCAAGGAGC CCATGGTTGC CCGGAAGCAG AAGCTGGCCG ATTCCCTGCG GTTGCAGCAG 
CTCTTCCGTG ACGTTGAGGA TGAGGAGACG TGGATTCGGG AGAAAGAACC TATTGCCGCC 
TCCACCAACA GAGGCAAAGA TTTAATTGGA GTCCAGAACC TGCTAAAGAA ACACCAAGCG 
CTACAAGCAG AAATTGCTGG CCACGAGCCC CGCATCAAAG CAGTCACACA GAAGGGGAAC 
GCCATGGTAG AGGAGGGCCA TTTTGCTGCC GAGGACGTGA AGGCCAAGCT CCATGAACTG 
AACCAGAAGT GGGAGTCGCT GAAGTCCAAG GCCTCCCAGC GACGGCAGGA CCTGGAGGAC 
TCGCTGCAGG CCCAGCAGTA CTTCGCTGAT GCCAACGAGG CCGAGTCCTG GATGCGGGAG 
AAGGAGCCCA TCGTGGGCAG CACCGACTAT GGCAAAGATG AGGACTCTGC CGAGGCTCTG 
CTAAAGAAGC ACGAGGCATT GATGTCGGAT CTCAGCGCCT ATGGCAGCAG CATCCAGGCC 
CTGCGAGAGC AGGCCCAGTC GTGCCGGCAA CAAGTGGCCC CCATGGATGA CGAGACTGGG 
AAGGAGCTGG TCTTGGCGCT CTATGACTAT CAGGAGAAGA GTCCCCGAGA GGTCACCATG 
AAGAAAGGAG ACATTCTCAC CTTACTCAAC AGCACCAACA AGGACTGGTG GAAAGTGGAA 
GTGAACGACC GGCAGGGCTT CGTGCCGGCC GCCTACGTGA AGAAGCTGGA CCCAGCGCAG 
TCGGCCTCCC GGGAGAACCT CCTGGAAGAG CAGGGCAGCA TCGCGCTGCG GCAGGAGCAG 
ATTGACAACC AGACGCGCGT AACTAAGGAG GCCGGCAGCG TGTCTCTGCG TATGAAACAG 
GTGGAAGAAC TGTACCATTC TCTGCTGGAA CTGGGAGAGA AGCGTAAAGG CATGTTGGAG 
AAGAGCTGTA AGAAGTTCAT GCTGTTCCGT GAAGCGAACG AGCTGCAGCA GTGGATCAAC 
GAGAAGGAAG CGGCCCTGAC AAGCGAGGAG GTCGGCGCGG ACTTGGAGCA GGTCGAAGTG 
CTGCAGAAGA AGTTTGACGA CTTCCAGAAG GATCTCAAGG CCAATGAGTC ACGGCTGAAG 
GACATTAACA AGGTGGCTGA AGACCTGGAG TCGGAAGGCC TGATGGCGGA GGAGGTGCAG 
GCCGTGCAGC AGCAGGAGGT GTATGGCGCG ATGCCCAGGG ATGAATCTGA TTCCAAGACA 
GCCTCCCCGT GGAAGTCTGC TCGCCTGATG GTTCACACCG TGGCCACCTT TAATTCCATC 
AAGGAGCTCA ATGAGCGCTG GCGGTCCCTG CAGCAGCTGG CTGAGGAGCG GAGCCAACTC 
TTGGGCAGCG CCCACGAAGT GCAGCGGTTC CACAGAGATG CTGACGAAAC CAAAGAGTGG 
ATCGAGGAGA AGAACCAGGC TCTAAACACA GACAACTATG GCCACGACCT GGCCAGCGTC 
CAGGCCCTGC AGCGCAAGCA TGAGGGCTTC GAGAGGGACC TCGCGGCCCT TGGCGACAAG 
GTGAACTCGC TCGGGGAGAC AGCCGAGCGC CTGATCCAGT CCCACCCCGA GTCGGCAGAG 
GACCTGCAGG AGAAGTGCAC TGAGCTGAAC CAGGCATGGA GCAGCCTGGG CAAGCGTGCC 
GACCAGCGCA AGGCCAAGCT GGGCGACTCC CACGACCTGC AGCGCTTCCT CAGCGACTTC 
CGGGACCTCA TGTCTTGGAT CAATGGAATC CGAGGTCTGG TGTCCTCAGA CGAGCTAGCC 
AAGGATGTCA CTGGAGCTGA GGCCCTGCTG GAGCGACACC AGGAACACCG GACAGAAATT 
GATGCCAGAG CTGGCACTTT CCAGGCATTC GAGCAGTTTG GGCAGCAGCT GTTGGCTCAT 
GGGCACTACG CCAGCCCCGA GATCAAGGAG AAGCTCGATG TTCTAGACCA GGAGCGCGCA 
GACCTCGAGA AGGCCTGGGT CCAGCGCAGG ATGATGCTGG ATCAGTGCCT TGAATTGCAG 
CTGTTCCATC GGGATTGCGA GCAGGCCGAG AACTGGATGG CTGCCCGGGA GGCCTTCCTG 
AACACAGAAG ACAAAGGGGA CTCGCTAGAC AGCGTGGAGG CTCTGATCAA AAAACACGAG 
GACTTTGACA AAGCAATTAA TGTCCAGGAG GAGAAGATTG CTGCCCTGCA GTCCTTTGCT 
GACCAGCTCA TTGCTGGCGG TCACTACGCC AAAGGCGACA TCTCCAGCCG GCGCAATGAG 
GTTCTGGACA GGTGGCGCCG TCTAAAAGCT CAGATGATTG AGAAGAGGTC AAAGCTGGGA 
GAATCGCAGA CCCTCCAGCA GTTCAGCCGG GATGTAGACG AGATTGAAGC TTGGATCAGT 
GAAAAATTAC AGACTGCAAG TGATGAGTCA TATAAGGACC CCACCAACAT CCAGAGCAAG 
CATCAGAAGC ACCAGGCCTT CGAGGCAGAA CTGCACGCCA ATGCGGACCG GATCCGCGGG 
GTCATCGACG TGGGCAACTC CCTCATTGAC CGTGGGGCCT GTGCCGGCAG CGAGGATGCC 
GTCAAGGCCC GGCTGGCCGC CTTAGCTGAC CAGTGGCAGT TCCTGGTGCA GAAGTCGGCT 
GAGAAGAGCC AGAAGCTGAA AGAAGCCAAC AAACAGCAGA ACTTCAACAC TGGCATCAAG 
GACTTCGACT TCTGGCTCTC TGAGGTGGAA GCCCTGCTGG CCTCTGAGGA TTATGGCAAA 
GACCTGGCTT CTGTGAACAA CTTGCTAAAA AAACATCAGC TGCTGGAGGC AGATATCTCT 
GCCCACGAGG ACCGCCTGAA GGACCTCAAC AGCCAGGCGG ACAGCCTGAT GACCAGCAGC 
GCCTTCGACA CCTCCCAGGT GAAGGATAAG CGGGACACCA TCAACAGCCG TTTCCAGAAG 
ATCAAGAGCA TGGCCGCCTC CCGGCGAGCT AGGCTGAATG AGTCCCACCG CCTGCACCAG 
TTCTTCCGGG ACATGGACGA TGAGGAGTCC TGGATCAAGG AGAAGAAGCT GCTGGTGAGC 
TCAGAGGACT ACGGCCGCGA CCTGACGGGC GTCCAGAACC TGAGGAAGAA GCACAAACGG 
CTGGAGGCAG AGCTGGCTGC CCACGAGCCG GCCATTCAGG GTGTCCTGGA CACTGGAAAG 
AAGCTGTCTG ATGACAACAC CATCGGGAAG GAGGAGATTC AGCAGCGCCT GGCGCAGTTT 
GTGGAGCACT GGAAGGAGCT GAAGCAGCTG GCAGCTGCTC GGGGCCAGCG GCTAGAAGAA 
TCTTTGGAAT ATCAGCAGTT TGTGGCCAAC GTGGAGGAGG AGGAAGCCTG GATCAATGAG 
AAGATGACCC TGGTGGCCAG TGAGGATTAC GGAGACACTC TTGCTGCCAT CCAGGGCTTG 
CTGAAGAAAC ACGAAGCCTT CGAGACGGAC TTCACTGTCC ACAAGGACCG GGTGAATGAC 
GTCTGCACTA ACGGACAAGA CCTGGTTAAG AAGAACAATC ACCATGAGGA GAACATCTCT 
TCCAAGATGA AGGGCTTGAA CGGGAAAGTG TCAGACTTGG AGAAGGCTGC CTCCCAGAGG 
AAGGCAAAGC TCGACGAGAA CTCGGCCTTC CTCCAGTTCA ACTGGAAGGC CGATGTGGTG 
GAGTCCTGGA TTGGTGAAAA GGAGAACAGC TTGAAGACAG ATGATTATGG TCGAGATCTG 
TCTTCTGTCC AAACTCTCCT CACCAAACAG GAAACCTTCG ATGCTGGCCT GCAGGCCTTC 
CAGCAGGAGG GCATCGCCAA CATCACTGCC CTCAAAGATC AGCTGCTGGC CGCCAAGCAT 
GTTCAGTCCA AGGCCATCGA GGCCCGCCAC GCTGCCCTCA TGAAGAGATG GAGCCAGCTC 
CTGGCCAATT CGGCCACCCG GAAGAAGAAG CTGCTGGAGG CACAGAGTCA CTTCCGCAAG 
GTGGAGGATC TCTTCCTGAC CTTTGCCAAA AAGGCTTCAG CCTTCAACAG CTGGTTTGAA 
AATGCAGAGG AGGACTTAAC AGACCCAGTG CGCTGCAACT CCCTGGAAGA GATCAAGGCC 
TTGAGAGAGG CCCACGATGC CTTCCGCTCC TCGCTGAGCT CAGCCCAGGC CGACTTCAAC 
CAGCTGGCTG AGCTGGACCG CCAGATCAAG AGCTTCCGCG TGGCCTCCAA CCCCTACACC 
TGGTTCACCA TGGAGGCCCT GGAGGAGACC TGGAGGAACC TTCAGAAGAT CATCAAGGAA 
AGGGAGCTGG AGCTGCAGAA GGAGCAGCGG CGGCAGGAGG AGAACGACAA GCTGCGCCAG 
GAGTTCGCCC AGCATGCCAA CGCCTTCCAC CAGTGGATCC AGGAGACCAG AACGTATCTC 
CTCGATGGGT CCTGCATGGT GGAAGAATCT GGGACCCTTG AATCGCAGCT TGAAGCTACC 
AAACGCAAAC ACCAGGAGAT TCGAGCCATG AGAAGTCAGC TCAAGAAGAT TGAAGACCTG 
GGGGCGGCCA TGGAGGAAGC GCTGATCCTG GACAACAAGT ACACGGAGCA CAGCACCGTG 
GGCCTGGCCC AGCAGTGGGA CCAGCTCGAC CAGCTGGGCA TGCGGATGCA GCACAACCTG 
GAGCAGCAGA TCCAGGCCAG GAACACGACA GGAGTGACCG AGGAGGCCCT CAAAGAATTC 
AGCATGATGT TCAAACACTT CGACAAGGAC AAGTCCGGCC GGCTGAATCA CCAGGAGTTC 
AAATCCTGTC TGCGCTCCCT GGGCTATGAC CTGCCCATGG TGGAGGAAGG GGAGCCCGAC 
CCTGAGTTCG AGGCCATCTT GGATACTGTG GATCCTAACA GGGACGGCCA CGTCTCCCTG 
CAAGAGTACA TGGCCTTTAT GATCAGCCGT GAGACCGAGA ATGTCAAGTC CAGCGAGGAG 
ATTGAGAGCG CCTTCCGGGC CCTGAGCTCC GAAGGGAAGC CTTATGTGAC CAAGGAGGAG 
CTGTACCAGA ACCTGACTCG GGAACAAGCC GACTACTGCG TCTCCCACAT GAAGCCCTAC 
GTGGACGGCA AGGGCCGCGA ACTCCCCACT GCTTTCGACT ACGTTGAGTT CACCCGCTCG 
CTCTTCGTGA ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTAN1 ( XM_012147486.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_012002876.1 Ovis aries musimon spectrin, alpha, non-erythrocytic 1 (SPTAN1), transcript variant X10, mRNA.

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CloneID	OOv24456
Clone ID Related Accession (Same CDS sequence)	XM_012147486.1
Accession Version	XM_012147486.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7452bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	spectrin alpha chain, non-erythrocytic 1 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942583.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTCAT CGTTTCGTAA ACGCAGAGTC CAGAAAATGG ACCCAAGCGG GGTCAAAGTG 
CTGGAGACAG CCGAGGACAT CCAGGAGAGG CGGCAGCAGG TCCTGGACAG ATACCACCGC 
TTCAAGGAGC TCTCGACCCT GAGGCGCCAG AAGCTTGAAG ACTCCTACCG ATTCCAGTTT 
TTCCAAAGAG ATGCTGAGGA GCTGGAGAAA TGGATTCAGG AGAAGCTTCA GATTGCCTCG 
GATGAGAATT ACAAAGACCC AACCAACCTG CAGGGCAAGC TGCAGAAGCA TCAAGCCTTC 
GAAGCCGAGG TGCAGGCCAA CTCGGGTGCC ATCGTGAAGC TTGATGAGAC CGGTAACCTG 
ATGATCTCAG AAGGGCACTT CGCGTCCGAA ACCATCCGGA CTCGTCTGAT GGAGCTGCAC 
CGCCAGTGGG AATTGCTTCT GGAGAAGATG CGGGAGAAAG GCATCAAGCT GCTGCAGGCC 
CAGAAGCTGG TGCAGTACCT GCGGGAGTGT GAGGACGTGA TGGACTGGAT CAATGACAAG 
GAAGCCATTG TGACATCTGA GGAGCTGGGC CAGGACCTGG AGCACGTGGA GGTGTTACAG 
AAGAAATTCG AAGAGTTTCA GACAGACATG GCTGCCCACG AAGAGAGAGT CAATGAAGTG 
AACCAGTTTG CTGCCAAGCT CATTCAGGAG CAGCACCCTG AGGAGGAGCT GATCAAGACA 
AAGGAGGACG AGGTGAATGC AGCCTGGCAG CGGCTGAAGG GCCTGGCCCT CCAGCGGCAG 
GGGAAGCTGT TCGGGGCAGC TGAGGTCCAA CGCTTTAACA GGGATGTGGA CGAGACCATT 
AGCTGGATTA AGGAGAAGGA ACAGTTAATG GCCTCTGATG ACTTTGGCCG GGACCTGGCC 
AGTGTCCAGG CTCTACTGCG GAAACACGAG GGTTTGGAGA GAGATCTTGC TGCCTTGGAG 
GACAAGGTCA AAGCCCTGTG TGCCGAGGCT GACCGCCTGC AGCAGTCCCA CCCTCTGAGT 
GCCACCCAGA TCCAAGTGAA GCGAGAGGAG CTGATCACCA ACTGGGAGCA GATCCGCACC 
CTGGCCGCGG AGAGACACGC ACGTCTCAAC GATTCCTACA GGCTTCAGCG CTTCCTTGCT 
GACTTCCGAG ACCTCACCAG CTGGGTCACT GAGATGAAAG CCCTCATCAA TGCAGATGAA 
CTTGCCAATG ATGTGGCCGG GGCCGAAGCC CTGCTGGATA GGCATCAGGA GCACAAGGGT 
GAAATTGACG CTCATGAAGA TAGCTTTAAA TCTGCAGATG AGTCTGGACA GGCCCTGCTT 
GCAGCTGGTC ACTATGCCTC AGATGAAGTG AGGGAGAAGC TGACCATCCT GTCCGAGGAG 
CGGGCCGCGC TACTAGAGCT CTGGGAGCTG CGACGGCAGC AATATGAGCA GTGCATGGAC 
CTGCAGCTCT TCTACCGCGA CACGGAGCAG GTGGACAACT GGATGAGCAA GCAGGAGGCG 
TTCCTGTTGA ACGAAGACCT GGGTGATTCC TTGGATAGTG TGGAAGCGCT TCTCAAGAAG 
CACGAAGACT TTGAGAAGTC CCTCAGCGCC CAGGAGGAGA AGATCACAGC CTTAGATGAG 
TTTGCCACCA AGCTGATTCA GAACAACCAC TACGCGATGG AAGATGTGGC CACCCGGCGA 
GACGCACTGC TGAGCCGCCG CAACGCCCTC CATGAGAGAG CCATGTACCG CCGTGCCCAG 
CTTGCAGATT CCTTCCACTT GCAGCAGTTT TTCCGTGACT CTGATGAGCT CAAGAGTTGG 
GTCAACGAGA AGATGAAAAC AGCCACAGAT GAAGCTTATA AAGACCCATC CAACCTACAA 
GGGAAAGTGC AGAAGCACCA GGCCTTTGAG GCCGAGCTCT CAGCAAACCA GAGCCGCATC 
GACGCCTTGG AGAAAGCTGG GCAGAAGCTG ATCGACGTTG ACCACTATGC CAAGGATGAG 
GTGGCGACTC GGATGAATGA AGTTATCAGT CTGTGGAAGA AGCTGCTGGA GGCCACGGAG 
CTGAAAGGCA TAAAGCTCCG TGAGGCTAAC CAGCAGCAGC AGTTTAACCG CAATGTTGAG 
GACATCGAGC TGTGGCTGTA CGAAGTGGAA GGTCATCTGG CCTCGGATGA CTACGGCAAA 
GACCTCACCA ACGTCCAGAA CCTCCAGAAA AAGCATGCCC TGCTGGAGGC TGACGTGGCC 
GCTCACCAGG ATCGCATCGA TGGCATCACT ATCCAAGCCC GCCAGTTCCA GGATGCGGGC 
CACTTTGATG CTGAAAACAT CAAGAAGAAG CAGGAGGCCC TGGTAGCTCG CTACGAGGCA 
CTCAAGGAGC CCATGGTTGC CCGGAAGCAG AAGCTGGCCG ATTCCCTGCG GTTGCAGCAG 
CTCTTCCGTG ACGTTGAGGA TGAGGAGACG TGGATTCGGG AGAAAGAACC TATTGCCGCC 
TCCACCAACA GAGGCAAAGA TTTAATTGGA GTCCAGAACC TGCTAAAGAA ACACCAAGCG 
CTACAAGCAG AAATTGCTGG CCACGAGCCC CGCATCAAAG CAGTCACACA GAAGGGGAAC 
GCCATGGTAG AGGAGGGCCA TTTTGCTGCC GAGGACGTGA AGGCCAAGCT CCATGAACTG 
AACCAGAAGT GGGAGTCGCT GAAGTCCAAG GCCTCCCAGC GACGGCAGGA CCTGGAGGAC 
TCGCTGCAGG CCCAGCAGTA CTTCGCTGAT GCCAACGAGG CCGAGTCCTG GATGCGGGAG 
AAGGAGCCCA TCGTGGGCAG CACCGACTAT GGCAAAGATG AGGACTCTGC CGAGGCTCTG 
CTAAAGAAGC ACGAGGCATT GATGTCGGAT CTCAGCGCCT ATGGCAGCAG CATCCAGGCC 
CTGCGAGAGC AGGCCCAGTC GTGCCGGCAA CAAGTGGCCC CCATGGATGA CGAGACTGGG 
AAGGAGCTGG TCTTGGCGCT CTATGACTAT CAGGAGAAGA GTCCCCGAGA GGTCACCATG 
AAGAAAGGAG ACATTCTCAC CTTACTCAAC AGCACCAACA AGGACTGGTG GAAAGTGGAA 
GTGAACGACC GGCAGGGCTT CGTGCCGGCC GCCTACGTGA AGAAGCTGGA CCCAGCGCAG 
TCGGCCTCCC GGGAGAACCT CCTGGAAGAG CAGGGCAGCA TCGCGCTGCG GCAGGAGCAG 
ATTGACAACC AGACGCGCGT AACTAAGGAG GCCGGCAGCG TGTCTCTGCG TATGAAACAG 
GTGGAAGAAC TGTACCATTC TCTGCTGGAA CTGGGAGAGA AGCGTAAAGG CATGTTGGAG 
AAGAGCTGTA AGAAGTTCAT GCTGTTCCGT GAAGCGAACG AGCTGCAGCA GTGGATCAAC 
GAGAAGGAAG CGGCCCTGAC AAGCGAGGAG GTCGGCGCGG ACTTGGAGCA GGTCGAAGTG 
CTGCAGAAGA AGTTTGACGA CTTCCAGAAG GATCTCAAGG CCAATGAGTC ACGGCTGAAG 
GACATTAACA AGGTGGCTGA AGACCTGGAG TCGGAAGGCC TGATGGCGGA GGAGGTGCAG 
GCCGTGCAGC AGCAGGAGGT GTATGGCGCG ATGCCCAGGG ATGAATCTGA TTCCAAGACA 
GCCTCCCCGT GGAAGTCTGC TCGCCTGATG GTTCACACCG TGGCCACCTT TAATTCCATC 
AAGGAGCTCA ATGAGCGCTG GCGGTCCCTG CAGCAGCTGG CTGAGGAGCG GAGCCAACTC 
TTGGGCAGCG CCCACGAAGT GCAGCGGTTC CACAGAGATG CTGACGAAAC CAAAGAGTGG 
ATCGAGGAGA AGAACCAGGC TCTAAACACA GACAACTATG GCCACGACCT GGCCAGCGTC 
CAGGCCCTGC AGCGCAAGCA TGAGGGCTTC GAGAGGGACC TCGCGGCCCT TGGCGACAAG 
GTGAACTCGC TCGGGGAGAC AGCCGAGCGC CTGATCCAGT CCCACCCCGA GTCGGCAGAG 
GACCTGCAGG AGAAGTGCAC TGAGCTGAAC CAGGCATGGA GCAGCCTGGG CAAGCGTGCC 
GACCAGCGCA AGGCCAAGCT GGGCGACTCC CACGACCTGC AGCGCTTCCT CAGCGACTTC 
CGGGACCTCA TGTCTTGGAT CAATGGAATC CGAGGTCTGG TGTCCTCAGA CGAGCTAGCC 
AAGGATGTCA CTGGAGCTGA GGCCCTGCTG GAGCGACACC AGGAACACCG GACAGAAATT 
GATGCCAGAG CTGGCACTTT CCAGGCATTC GAGCAGTTTG GGCAGCAGCT GTTGGCTCAT 
GGGCACTACG CCAGCCCCGA GATCAAGGAG AAGCTCGATG TTCTAGACCA GGAGCGCGCA 
GACCTCGAGA AGGCCTGGGT CCAGCGCAGG ATGATGCTGG ATCAGTGCCT TGAATTGCAG 
CTGTTCCATC GGGATTGCGA GCAGGCCGAG AACTGGATGG CTGCCCGGGA GGCCTTCCTG 
AACACAGAAG ACAAAGGGGA CTCGCTAGAC AGCGTGGAGG CTCTGATCAA AAAACACGAG 
GACTTTGACA AAGCAATTAA TGTCCAGGAG GAGAAGATTG CTGCCCTGCA GTCCTTTGCT 
GACCAGCTCA TTGCTGGCGG TCACTACGCC AAAGGCGACA TCTCCAGCCG GCGCAATGAG 
GTTCTGGACA GGTGGCGCCG TCTAAAAGCT CAGATGATTG AGAAGAGGTC AAAGCTGGGA 
GAATCGCAGA CCCTCCAGCA GTTCAGCCGG GATGTAGACG AGATTGAAGC TTGGATCAGT 
GAAAAATTAC AGACTGCAAG TGATGAGTCA TATAAGGACC CCACCAACAT CCAGCTTTCC 
AAGCTGCTGA GCAAGCATCA GAAGCACCAG GCCTTCGAGG CAGAACTGCA CGCCAATGCG 
GACCGGATCC GCGGGGTCAT CGACGTGGGC AACTCCCTCA TTGACCGTGG GGCCTGTGCC 
GGCAGCGAGG ATGCCGTCAA GGCCCGGCTG GCCGCCTTAG CTGACCAGTG GCAGTTCCTG 
GTGCAGAAGT CGGCTGAGAA GAGCCAGAAG CTGAAAGAAG CCAACAAACA GCAGAACTTC 
AACACTGGCA TCAAGGACTT CGACTTCTGG CTCTCTGAGG TGGAAGCCCT GCTGGCCTCT 
GAGGATTATG GCAAAGACCT GGCTTCTGTG AACAACTTGC TAAAAAAACA TCAGCTGCTG 
GAGGCAGATA TCTCTGCCCA CGAGGACCGC CTGAAGGACC TCAACAGCCA GGCGGACAGC 
CTGATGACCA GCAGCGCCTT CGACACCTCC CAGGTGAAGG ATAAGCGGGA CACCATCAAC 
AGCCGTTTCC AGAAGATCAA GAGCATGGCC GCCTCCCGGC GAGCTAGGCT GAATGAGTCC 
CACCGCCTGC ACCAGTTCTT CCGGGACATG GACGATGAGG AGTCCTGGAT CAAGGAGAAG 
AAGCTGCTGG TGAGCTCAGA GGACTACGGC CGCGACCTGA CGGGCGTCCA GAACCTGAGG 
AAGAAGCACA AACGGCTGGA GGCAGAGCTG GCTGCCCACG AGCCGGCCAT TCAGGGTGTC 
CTGGACACTG GAAAGAAGCT GTCTGATGAC AACACCATCG GGAAGGAGGA GATTCAGCAG 
CGCCTGGCGC AGTTTGTGGA GCACTGGAAG GAGCTGAAGC AGCTGGCAGC TGCTCGGGGC 
CAGCGGCTAG AAGAATCTTT GGAATATCAG CAGTTTGTGG CCAACGTGGA GGAGGAGGAA 
GCCTGGATCA ATGAGAAGAT GACCCTGGTG GCCAGTGAGG ATTACGGAGA CACTCTTGCT 
GCCATCCAGG GCTTGCTGAA GAAACACGAA GCCTTCGAGA CGGACTTCAC TGTCCACAAG 
GACCGGGTGA ATGACGTCTG CACTAACGGA CAAGACCTGG TTAAGAAGAA CAATCACCAT 
GAGGAGAACA TCTCTTCCAA GATGAAGGGC TTGAACGGGA AAGTGTCAGA CTTGGAGAAG 
GCTGCCTCCC AGAGGAAGGC AAAGCTCGAC GAGAACTCGG CCTTCCTCCA GTTCAACTGG 
AAGGCCGATG TGGTGGAGTC CTGGATTGGT GAAAAGGAGA ACAGCTTGAA GACAGATGAT 
TATGGTCGAG ATCTGTCTTC TGTCCAAACT CTCCTCACCA AACAGGAAAC CTTCGATGCT 
GGCCTGCAGG CCTTCCAGCA GGAGGGCATC GCCAACATCA CTGCCCTCAA AGATCAGCTG 
CTGGCCGCCA AGCATGTTCA GTCCAAGGCC ATCGAGGCCC GCCACGCTGC CCTCATGAAG 
AGATGGAGCC AGCTCCTGGC CAATTCGGCC ACCCGGAAGA AGAAGCTGCT GGAGGCACAG 
AGTCACTTCC GCAAGGTGGA GGATCTCTTC CTGACCTTTG CCAAAAAGGC TTCAGCCTTC 
AACAGCTGGT TTGAAAATGC AGAGGAGGAC TTAACAGACC CAGTGCGCTG CAACTCCCTG 
GAAGAGATCA AGGCCTTGAG AGAGGCCCAC GATGCCTTCC GCTCCTCGCT GAGCTCAGCC 
CAGGCCGACT TCAACCAGCT GGCTGAGCTG GACCGCCAGA TCAAGAGCTT CCGCGTGGCC 
TCCAACCCCT ACACCTGGTT CACCATGGAG GCCCTGGAGG AGACCTGGAG GAACCTTCAG 
AAGATCATCA AGGAAAGGGA GCTGGAGCTG CAGAAGGAGC AGCGGCGGCA GGAGGAGAAC 
GACAAGCTGC GCCAGGAGTT CGCCCAGCAT GCCAACGCCT TCCACCAGTG GATCCAGGAG 
ACCAGGTCCT GCATGGTGGA AGAATCTGGG ACCCTTGAAT CGCAGCTTGA AGCTACCAAA 
CGCAAACACC AGGAGATTCG AGCCATGAGA AGTCAGCTCA AGAAGATTGA AGACCTGGGG 
GCGGCCATGG AGGAAGCGCT GATCCTGGAC AACAAGTACA CGGAGCACAG CACCGTGGGC 
CTGGCCCAGC AGTGGGACCA GCTCGACCAG CTGGGCATGC GGATGCAGCA CAACCTGGAG 
CAGCAGATCC AGGCCAGGAA CACGACAGGA GTGACCGAGG AGGCCCTCAA AGAATTCAGC 
ATGATGTTCA AACACTTCGA CAAGGACAAG TCCGGCCGGC TGAATCACCA GGAGTTCAAA 
TCCTGTCTGC GCTCCCTGGG CTATGACCTG CCCATGGTGG AGGAAGGGGA GCCCGACCCT 
GAGTTCGAGG CCATCTTGGA TACTGTGGAT CCTAACAGGG ACGGCCACGT CTCCCTGCAA 
GAGTACATGG CCTTTATGAT CAGCCGTGAG ACCGAGAATG TCAAGTCCAG CGAGGAGATT 
GAGAGCGCCT TCCGGGCCCT GAGCTCCGAA GGGAAGCCTT ATGTGACCAA GGAGGAGCTG 
TACCAGAACC TGACTCGGGA ACAAGCCGAC TACTGCGTCT CCCACATGAA GCCCTACGTG 
GACGGCAAGG GCCGCGAACT CCCCACTGCT TTCGACTACG TTGAGTTCAC CCGCTCGCTC 
TTCGTGAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012002876.1
CDS	81..7532
Translation	MLSSFRKRRVQKMDPSGVKVLETAEDIQERRQQVLDRYHRFKELSTLRRQKLEDSYRFQFFQRDAEELEKWIQEKLQIASDENYKDPTNLQGKLQKHQAFEAEVQANSGAIVKLDETGNLMISEGHFASETIRTRLMELHRQWELLLEKMREKGIKLLQAQKLVQYLRECEDVMDWINDKEAIVTSEELGQDLEHVEVLQKKFEEFQTDMAAHEERVNEVNQFAAKLIQEQHPEEELIKTKEDEVNAAWQRLKGLALQRQGKLFGAAEVQRFNRDVDETISWIKEKEQLMASDDFGRDLASVQALLRKHEGLERDLAALEDKVKALCAEADRLQQSHPLSATQIQVKREELITNWEQIRTLAAERHARLNDSYRLQRFLADFRDLTSWVTEMKALINADELANDVAGAEALLDRHQEHKGEIDAHEDSFKSADESGQALLAAGHYASDEVREKLTILSEERAALLELWELRRQQYEQCMDLQLFYRDTEQVDNWMSKQEAFLLNEDLGDSLDSVEALLKKHEDFEKSLSAQEEKITALDEFATKLIQNNHYAMEDVATRRDALLSRRNALHERAMYRRAQLADSFHLQQFFRDSDELKSWVNEKMKTATDEAYKDPSNLQGKVQKHQAFEAELSANQSRIDALEKAGQKLIDVDHYAKDEVATRMNEVISLWKKLLEATELKGIKLREANQQQQFNRNVEDIELWLYEVEGHLASDDYGKDLTNVQNLQKKHALLEADVAAHQDRIDGITIQARQFQDAGHFDAENIKKKQEALVARYEALKEPMVARKQKLADSLRLQQLFRDVEDEETWIREKEPIAASTNRGKDLIGVQNLLKKHQALQAEIAGHEPRIKAVTQKGNAMVEEGHFAAEDVKAKLHELNQKWESLKSKASQRRQDLEDSLQAQQYFADANEAESWMREKEPIVGSTDYGKDEDSAEALLKKHEALMSDLSAYGSSIQALREQAQSCRQQVAPMDDETGKELVLALYDYQEKSPREVTMKKGDILTLLNSTNKDWWKVEVNDRQGFVPAAYVKKLDPAQSASRENLLEEQGSIALRQEQIDNQTRVTKEAGSVSLRMKQVEELYHSLLELGEKRKGMLEKSCKKFMLFREANELQQWINEKEAALTSEEVGADLEQVEVLQKKFDDFQKDLKANESRLKDINKVAEDLESEGLMAEEVQAVQQQEVYGAMPRDESDSKTASPWKSARLMVHTVATFNSIKELNERWRSLQQLAEERSQLLGSAHEVQRFHRDADETKEWIEEKNQALNTDNYGHDLASVQALQRKHEGFERDLAALGDKVNSLGETAERLIQSHPESAEDLQEKCTELNQAWSSLGKRADQRKAKLGDSHDLQRFLSDFRDLMSWINGIRGLVSSDELAKDVTGAEALLERHQEHRTEIDARAGTFQAFEQFGQQLLAHGHYASPEIKEKLDVLDQERADLEKAWVQRRMMLDQCLELQLFHRDCEQAENWMAAREAFLNTEDKGDSLDSVEALIKKHEDFDKAINVQEEKIAALQSFADQLIAGGHYAKGDISSRRNEVLDRWRRLKAQMIEKRSKLGESQTLQQFSRDVDEIEAWISEKLQTASDESYKDPTNIQLSKLLSKHQKHQAFEAELHANADRIRGVIDVGNSLIDRGACAGSEDAVKARLAALADQWQFLVQKSAEKSQKLKEANKQQNFNTGIKDFDFWLSEVEALLASEDYGKDLASVNNLLKKHQLLEADISAHEDRLKDLNSQADSLMTSSAFDTSQVKDKRDTINSRFQKIKSMAASRRARLNESHRLHQFFRDMDDEESWIKEKKLLVSSEDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQFVEHWKELKQLAAARGQRLEESLEYQQFVANVEEEEAWINEKMTLVASEDYGDTLAAIQGLLKKHEAFETDFTVHKDRVNDVCTNGQDLVKKNNHHEENISSKMKGLNGKVSDLEKAASQRKAKLDENSAFLQFNWKADVVESWIGEKENSLKTDDYGRDLSSVQTLLTKQETFDAGLQAFQQEGIANITALKDQLLAAKHVQSKAIEARHAALMKRWSQLLANSATRKKKLLEAQSHFRKVEDLFLTFAKKASAFNSWFENAEEDLTDPVRCNSLEEIKALREAHDAFRSSLSSAQADFNQLAELDRQIKSFRVASNPYTWFTMEALEETWRNLQKIIKERELELQKEQRRQEENDKLRQEFAQHANAFHQWIQETRSCMVEESGTLESQLEATKRKHQEIRAMRSQLKKIEDLGAAMEEALILDNKYTEHSTVGLAQQWDQLDQLGMRMQHNLEQQIQARNTTGVTEEALKEFSMMFKHFDKDKSGRLNHQEFKSCLRSLGYDLPMVEEGEPDPEFEAILDTVDPNRDGHVSLQEYMAFMISRETENVKSSEEIESAFRALSSEGKPYVTKEELYQNLTREQADYCVSHMKPYVDGKGRELPTAFDYVEFTRSLFVN

Target ORF information:

RefSeq Version	XM_012147486.1
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon spectrin, alpha, non-erythrocytic 1 (SPTAN1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012147486.1

ORF Insert Sequence:

ATGCTGTCAT CGTTTCGTAA ACGCAGAGTC CAGAAAATGG ACCCAAGCGG GGTCAAAGTG 
CTGGAGACAG CCGAGGACAT CCAGGAGAGG CGGCAGCAGG TCCTGGACAG ATACCACCGC 
TTCAAGGAGC TCTCGACCCT GAGGCGCCAG AAGCTTGAAG ACTCCTACCG ATTCCAGTTT 
TTCCAAAGAG ATGCTGAGGA GCTGGAGAAA TGGATTCAGG AGAAGCTTCA GATTGCCTCG 
GATGAGAATT ACAAAGACCC AACCAACCTG CAGGGCAAGC TGCAGAAGCA TCAAGCCTTC 
GAAGCCGAGG TGCAGGCCAA CTCGGGTGCC ATCGTGAAGC TTGATGAGAC CGGTAACCTG 
ATGATCTCAG AAGGGCACTT CGCGTCCGAA ACCATCCGGA CTCGTCTGAT GGAGCTGCAC 
CGCCAGTGGG AATTGCTTCT GGAGAAGATG CGGGAGAAAG GCATCAAGCT GCTGCAGGCC 
CAGAAGCTGG TGCAGTACCT GCGGGAGTGT GAGGACGTGA TGGACTGGAT CAATGACAAG 
GAAGCCATTG TGACATCTGA GGAGCTGGGC CAGGACCTGG AGCACGTGGA GGTGTTACAG 
AAGAAATTCG AAGAGTTTCA GACAGACATG GCTGCCCACG AAGAGAGAGT CAATGAAGTG 
AACCAGTTTG CTGCCAAGCT CATTCAGGAG CAGCACCCTG AGGAGGAGCT GATCAAGACA 
AAGGAGGACG AGGTGAATGC AGCCTGGCAG CGGCTGAAGG GCCTGGCCCT CCAGCGGCAG 
GGGAAGCTGT TCGGGGCAGC TGAGGTCCAA CGCTTTAACA GGGATGTGGA CGAGACCATT 
AGCTGGATTA AGGAGAAGGA ACAGTTAATG GCCTCTGATG ACTTTGGCCG GGACCTGGCC 
AGTGTCCAGG CTCTACTGCG GAAACACGAG GGTTTGGAGA GAGATCTTGC TGCCTTGGAG 
GACAAGGTCA AAGCCCTGTG TGCCGAGGCT GACCGCCTGC AGCAGTCCCA CCCTCTGAGT 
GCCACCCAGA TCCAAGTGAA GCGAGAGGAG CTGATCACCA ACTGGGAGCA GATCCGCACC 
CTGGCCGCGG AGAGACACGC ACGTCTCAAC GATTCCTACA GGCTTCAGCG CTTCCTTGCT 
GACTTCCGAG ACCTCACCAG CTGGGTCACT GAGATGAAAG CCCTCATCAA TGCAGATGAA 
CTTGCCAATG ATGTGGCCGG GGCCGAAGCC CTGCTGGATA GGCATCAGGA GCACAAGGGT 
GAAATTGACG CTCATGAAGA TAGCTTTAAA TCTGCAGATG AGTCTGGACA GGCCCTGCTT 
GCAGCTGGTC ACTATGCCTC AGATGAAGTG AGGGAGAAGC TGACCATCCT GTCCGAGGAG 
CGGGCCGCGC TACTAGAGCT CTGGGAGCTG CGACGGCAGC AATATGAGCA GTGCATGGAC 
CTGCAGCTCT TCTACCGCGA CACGGAGCAG GTGGACAACT GGATGAGCAA GCAGGAGGCG 
TTCCTGTTGA ACGAAGACCT GGGTGATTCC TTGGATAGTG TGGAAGCGCT TCTCAAGAAG 
CACGAAGACT TTGAGAAGTC CCTCAGCGCC CAGGAGGAGA AGATCACAGC CTTAGATGAG 
TTTGCCACCA AGCTGATTCA GAACAACCAC TACGCGATGG AAGATGTGGC CACCCGGCGA 
GACGCACTGC TGAGCCGCCG CAACGCCCTC CATGAGAGAG CCATGTACCG CCGTGCCCAG 
CTTGCAGATT CCTTCCACTT GCAGCAGTTT TTCCGTGACT CTGATGAGCT CAAGAGTTGG 
GTCAACGAGA AGATGAAAAC AGCCACAGAT GAAGCTTATA AAGACCCATC CAACCTACAA 
GGGAAAGTGC AGAAGCACCA GGCCTTTGAG GCCGAGCTCT CAGCAAACCA GAGCCGCATC 
GACGCCTTGG AGAAAGCTGG GCAGAAGCTG ATCGACGTTG ACCACTATGC CAAGGATGAG 
GTGGCGACTC GGATGAATGA AGTTATCAGT CTGTGGAAGA AGCTGCTGGA GGCCACGGAG 
CTGAAAGGCA TAAAGCTCCG TGAGGCTAAC CAGCAGCAGC AGTTTAACCG CAATGTTGAG 
GACATCGAGC TGTGGCTGTA CGAAGTGGAA GGTCATCTGG CCTCGGATGA CTACGGCAAA 
GACCTCACCA ACGTCCAGAA CCTCCAGAAA AAGCATGCCC TGCTGGAGGC TGACGTGGCC 
GCTCACCAGG ATCGCATCGA TGGCATCACT ATCCAAGCCC GCCAGTTCCA GGATGCGGGC 
CACTTTGATG CTGAAAACAT CAAGAAGAAG CAGGAGGCCC TGGTAGCTCG CTACGAGGCA 
CTCAAGGAGC CCATGGTTGC CCGGAAGCAG AAGCTGGCCG ATTCCCTGCG GTTGCAGCAG 
CTCTTCCGTG ACGTTGAGGA TGAGGAGACG TGGATTCGGG AGAAAGAACC TATTGCCGCC 
TCCACCAACA GAGGCAAAGA TTTAATTGGA GTCCAGAACC TGCTAAAGAA ACACCAAGCG 
CTACAAGCAG AAATTGCTGG CCACGAGCCC CGCATCAAAG CAGTCACACA GAAGGGGAAC 
GCCATGGTAG AGGAGGGCCA TTTTGCTGCC GAGGACGTGA AGGCCAAGCT CCATGAACTG 
AACCAGAAGT GGGAGTCGCT GAAGTCCAAG GCCTCCCAGC GACGGCAGGA CCTGGAGGAC 
TCGCTGCAGG CCCAGCAGTA CTTCGCTGAT GCCAACGAGG CCGAGTCCTG GATGCGGGAG 
AAGGAGCCCA TCGTGGGCAG CACCGACTAT GGCAAAGATG AGGACTCTGC CGAGGCTCTG 
CTAAAGAAGC ACGAGGCATT GATGTCGGAT CTCAGCGCCT ATGGCAGCAG CATCCAGGCC 
CTGCGAGAGC AGGCCCAGTC GTGCCGGCAA CAAGTGGCCC CCATGGATGA CGAGACTGGG 
AAGGAGCTGG TCTTGGCGCT CTATGACTAT CAGGAGAAGA GTCCCCGAGA GGTCACCATG 
AAGAAAGGAG ACATTCTCAC CTTACTCAAC AGCACCAACA AGGACTGGTG GAAAGTGGAA 
GTGAACGACC GGCAGGGCTT CGTGCCGGCC GCCTACGTGA AGAAGCTGGA CCCAGCGCAG 
TCGGCCTCCC GGGAGAACCT CCTGGAAGAG CAGGGCAGCA TCGCGCTGCG GCAGGAGCAG 
ATTGACAACC AGACGCGCGT AACTAAGGAG GCCGGCAGCG TGTCTCTGCG TATGAAACAG 
GTGGAAGAAC TGTACCATTC TCTGCTGGAA CTGGGAGAGA AGCGTAAAGG CATGTTGGAG 
AAGAGCTGTA AGAAGTTCAT GCTGTTCCGT GAAGCGAACG AGCTGCAGCA GTGGATCAAC 
GAGAAGGAAG CGGCCCTGAC AAGCGAGGAG GTCGGCGCGG ACTTGGAGCA GGTCGAAGTG 
CTGCAGAAGA AGTTTGACGA CTTCCAGAAG GATCTCAAGG CCAATGAGTC ACGGCTGAAG 
GACATTAACA AGGTGGCTGA AGACCTGGAG TCGGAAGGCC TGATGGCGGA GGAGGTGCAG 
GCCGTGCAGC AGCAGGAGGT GTATGGCGCG ATGCCCAGGG ATGAATCTGA TTCCAAGACA 
GCCTCCCCGT GGAAGTCTGC TCGCCTGATG GTTCACACCG TGGCCACCTT TAATTCCATC 
AAGGAGCTCA ATGAGCGCTG GCGGTCCCTG CAGCAGCTGG CTGAGGAGCG GAGCCAACTC 
TTGGGCAGCG CCCACGAAGT GCAGCGGTTC CACAGAGATG CTGACGAAAC CAAAGAGTGG 
ATCGAGGAGA AGAACCAGGC TCTAAACACA GACAACTATG GCCACGACCT GGCCAGCGTC 
CAGGCCCTGC AGCGCAAGCA TGAGGGCTTC GAGAGGGACC TCGCGGCCCT TGGCGACAAG 
GTGAACTCGC TCGGGGAGAC AGCCGAGCGC CTGATCCAGT CCCACCCCGA GTCGGCAGAG 
GACCTGCAGG AGAAGTGCAC TGAGCTGAAC CAGGCATGGA GCAGCCTGGG CAAGCGTGCC 
GACCAGCGCA AGGCCAAGCT GGGCGACTCC CACGACCTGC AGCGCTTCCT CAGCGACTTC 
CGGGACCTCA TGTCTTGGAT CAATGGAATC CGAGGTCTGG TGTCCTCAGA CGAGCTAGCC 
AAGGATGTCA CTGGAGCTGA GGCCCTGCTG GAGCGACACC AGGAACACCG GACAGAAATT 
GATGCCAGAG CTGGCACTTT CCAGGCATTC GAGCAGTTTG GGCAGCAGCT GTTGGCTCAT 
GGGCACTACG CCAGCCCCGA GATCAAGGAG AAGCTCGATG TTCTAGACCA GGAGCGCGCA 
GACCTCGAGA AGGCCTGGGT CCAGCGCAGG ATGATGCTGG ATCAGTGCCT TGAATTGCAG 
CTGTTCCATC GGGATTGCGA GCAGGCCGAG AACTGGATGG CTGCCCGGGA GGCCTTCCTG 
AACACAGAAG ACAAAGGGGA CTCGCTAGAC AGCGTGGAGG CTCTGATCAA AAAACACGAG 
GACTTTGACA AAGCAATTAA TGTCCAGGAG GAGAAGATTG CTGCCCTGCA GTCCTTTGCT 
GACCAGCTCA TTGCTGGCGG TCACTACGCC AAAGGCGACA TCTCCAGCCG GCGCAATGAG 
GTTCTGGACA GGTGGCGCCG TCTAAAAGCT CAGATGATTG AGAAGAGGTC AAAGCTGGGA 
GAATCGCAGA CCCTCCAGCA GTTCAGCCGG GATGTAGACG AGATTGAAGC TTGGATCAGT 
GAAAAATTAC AGACTGCAAG TGATGAGTCA TATAAGGACC CCACCAACAT CCAGCTTTCC 
AAGCTGCTGA GCAAGCATCA GAAGCACCAG GCCTTCGAGG CAGAACTGCA CGCCAATGCG 
GACCGGATCC GCGGGGTCAT CGACGTGGGC AACTCCCTCA TTGACCGTGG GGCCTGTGCC 
GGCAGCGAGG ATGCCGTCAA GGCCCGGCTG GCCGCCTTAG CTGACCAGTG GCAGTTCCTG 
GTGCAGAAGT CGGCTGAGAA GAGCCAGAAG CTGAAAGAAG CCAACAAACA GCAGAACTTC 
AACACTGGCA TCAAGGACTT CGACTTCTGG CTCTCTGAGG TGGAAGCCCT GCTGGCCTCT 
GAGGATTATG GCAAAGACCT GGCTTCTGTG AACAACTTGC TAAAAAAACA TCAGCTGCTG 
GAGGCAGATA TCTCTGCCCA CGAGGACCGC CTGAAGGACC TCAACAGCCA GGCGGACAGC 
CTGATGACCA GCAGCGCCTT CGACACCTCC CAGGTGAAGG ATAAGCGGGA CACCATCAAC 
AGCCGTTTCC AGAAGATCAA GAGCATGGCC GCCTCCCGGC GAGCTAGGCT GAATGAGTCC 
CACCGCCTGC ACCAGTTCTT CCGGGACATG GACGATGAGG AGTCCTGGAT CAAGGAGAAG 
AAGCTGCTGG TGAGCTCAGA GGACTACGGC CGCGACCTGA CGGGCGTCCA GAACCTGAGG 
AAGAAGCACA AACGGCTGGA GGCAGAGCTG GCTGCCCACG AGCCGGCCAT TCAGGGTGTC 
CTGGACACTG GAAAGAAGCT GTCTGATGAC AACACCATCG GGAAGGAGGA GATTCAGCAG 
CGCCTGGCGC AGTTTGTGGA GCACTGGAAG GAGCTGAAGC AGCTGGCAGC TGCTCGGGGC 
CAGCGGCTAG AAGAATCTTT GGAATATCAG CAGTTTGTGG CCAACGTGGA GGAGGAGGAA 
GCCTGGATCA ATGAGAAGAT GACCCTGGTG GCCAGTGAGG ATTACGGAGA CACTCTTGCT 
GCCATCCAGG GCTTGCTGAA GAAACACGAA GCCTTCGAGA CGGACTTCAC TGTCCACAAG 
GACCGGGTGA ATGACGTCTG CACTAACGGA CAAGACCTGG TTAAGAAGAA CAATCACCAT 
GAGGAGAACA TCTCTTCCAA GATGAAGGGC TTGAACGGGA AAGTGTCAGA CTTGGAGAAG 
GCTGCCTCCC AGAGGAAGGC AAAGCTCGAC GAGAACTCGG CCTTCCTCCA GTTCAACTGG 
AAGGCCGATG TGGTGGAGTC CTGGATTGGT GAAAAGGAGA ACAGCTTGAA GACAGATGAT 
TATGGTCGAG ATCTGTCTTC TGTCCAAACT CTCCTCACCA AACAGGAAAC CTTCGATGCT 
GGCCTGCAGG CCTTCCAGCA GGAGGGCATC GCCAACATCA CTGCCCTCAA AGATCAGCTG 
CTGGCCGCCA AGCATGTTCA GTCCAAGGCC ATCGAGGCCC GCCACGCTGC CCTCATGAAG 
AGATGGAGCC AGCTCCTGGC CAATTCGGCC ACCCGGAAGA AGAAGCTGCT GGAGGCACAG 
AGTCACTTCC GCAAGGTGGA GGATCTCTTC CTGACCTTTG CCAAAAAGGC TTCAGCCTTC 
AACAGCTGGT TTGAAAATGC AGAGGAGGAC TTAACAGACC CAGTGCGCTG CAACTCCCTG 
GAAGAGATCA AGGCCTTGAG AGAGGCCCAC GATGCCTTCC GCTCCTCGCT GAGCTCAGCC 
CAGGCCGACT TCAACCAGCT GGCTGAGCTG GACCGCCAGA TCAAGAGCTT CCGCGTGGCC 
TCCAACCCCT ACACCTGGTT CACCATGGAG GCCCTGGAGG AGACCTGGAG GAACCTTCAG 
AAGATCATCA AGGAAAGGGA GCTGGAGCTG CAGAAGGAGC AGCGGCGGCA GGAGGAGAAC 
GACAAGCTGC GCCAGGAGTT CGCCCAGCAT GCCAACGCCT TCCACCAGTG GATCCAGGAG 
ACCAGGTCCT GCATGGTGGA AGAATCTGGG ACCCTTGAAT CGCAGCTTGA AGCTACCAAA 
CGCAAACACC AGGAGATTCG AGCCATGAGA AGTCAGCTCA AGAAGATTGA AGACCTGGGG 
GCGGCCATGG AGGAAGCGCT GATCCTGGAC AACAAGTACA CGGAGCACAG CACCGTGGGC 
CTGGCCCAGC AGTGGGACCA GCTCGACCAG CTGGGCATGC GGATGCAGCA CAACCTGGAG 
CAGCAGATCC AGGCCAGGAA CACGACAGGA GTGACCGAGG AGGCCCTCAA AGAATTCAGC 
ATGATGTTCA AACACTTCGA CAAGGACAAG TCCGGCCGGC TGAATCACCA GGAGTTCAAA 
TCCTGTCTGC GCTCCCTGGG CTATGACCTG CCCATGGTGG AGGAAGGGGA GCCCGACCCT 
GAGTTCGAGG CCATCTTGGA TACTGTGGAT CCTAACAGGG ACGGCCACGT CTCCCTGCAA 
GAGTACATGG CCTTTATGAT CAGCCGTGAG ACCGAGAATG TCAAGTCCAG CGAGGAGATT 
GAGAGCGCCT TCCGGGCCCT GAGCTCCGAA GGGAAGCCTT ATGTGACCAA GGAGGAGCTG 
TACCAGAACC TGACTCGGGA ACAAGCCGAC TACTGCGTCT CCCACATGAA GCCCTACGTG 
GACGGCAAGG GCCGCGAACT CCCCACTGCT TTCGACTACG TTGAGTTCAC CCGCTCGCTC 
TTCGTGAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTAN1 ( XM_012147487.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_012002877.1 Ovis aries musimon spectrin, alpha, non-erythrocytic 1 (SPTAN1), transcript variant X11, mRNA.

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CloneID	OOv24457
Clone ID Related Accession (Same CDS sequence)	XM_012147487.1
Accession Version	XM_012147487.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7410bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	spectrin alpha chain, non-erythrocytic 1 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942583.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTCAT CGTTTCGTAA ACGCAGAGTC CAGAAAATGG ACCCAAGCGG GGTCAAAGTG 
CTGGAGACAG CCGAGGACAT CCAGGAGAGG CGGCAGCAGG TCCTGGACAG ATACCACCGC 
TTCAAGGAGC TCTCGACCCT GAGGCGCCAG AAGCTTGAAG ACTCCTACCG ATTCCAGTTT 
TTCCAAAGAG ATGCTGAGGA GCTGGAGAAA TGGATTCAGG AGAAGCTTCA GATTGCCTCG 
GATGAGAATT ACAAAGACCC AACCAACCTG CAGGGCAAGC TGCAGAAGCA TCAAGCCTTC 
GAAGCCGAGG TGCAGGCCAA CTCGGGTGCC ATCGTGAAGC TTGATGAGAC CGGTAACCTG 
ATGATCTCAG AAGGGCACTT CGCGTCCGAA ACCATCCGGA CTCGTCTGAT GGAGCTGCAC 
CGCCAGTGGG AATTGCTTCT GGAGAAGATG CGGGAGAAAG GCATCAAGCT GCTGCAGGCC 
CAGAAGCTGG TGCAGTACCT GCGGGAGTGT GAGGACGTGA TGGACTGGAT CAATGACAAG 
GAAGCCATTG TGACATCTGA GGAGCTGGGC CAGGACCTGG AGCACGTGGA GGTGTTACAG 
AAGAAATTCG AAGAGTTTCA GACAGACATG GCTGCCCACG AAGAGAGAGT CAATGAAGTG 
AACCAGTTTG CTGCCAAGCT CATTCAGGAG CAGCACCCTG AGGAGGAGCT GATCAAGACA 
AAGGAGGACG AGGTGAATGC AGCCTGGCAG CGGCTGAAGG GCCTGGCCCT CCAGCGGCAG 
GGGAAGCTGT TCGGGGCAGC TGAGGTCCAA CGCTTTAACA GGGATGTGGA CGAGACCATT 
AGCTGGATTA AGGAGAAGGA ACAGTTAATG GCCTCTGATG ACTTTGGCCG GGACCTGGCC 
AGTGTCCAGG CTCTACTGCG GAAACACGAG GGTTTGGAGA GAGATCTTGC TGCCTTGGAG 
GACAAGGTCA AAGCCCTGTG TGCCGAGGCT GACCGCCTGC AGCAGTCCCA CCCTCTGAGT 
GCCACCCAGA TCCAAGTGAA GCGAGAGGAG CTGATCACCA ACTGGGAGCA GATCCGCACC 
CTGGCCGCGG AGAGACACGC ACGTCTCAAC GATTCCTACA GGCTTCAGCG CTTCCTTGCT 
GACTTCCGAG ACCTCACCAG CTGGGTCACT GAGATGAAAG CCCTCATCAA TGCAGATGAA 
CTTGCCAATG ATGTGGCCGG GGCCGAAGCC CTGCTGGATA GGCATCAGGA GCACAAGGGT 
GAAATTGACG CTCATGAAGA TAGCTTTAAA TCTGCAGATG AGTCTGGACA GGCCCTGCTT 
GCAGCTGGTC ACTATGCCTC AGATGAAGTG AGGGAGAAGC TGACCATCCT GTCCGAGGAG 
CGGGCCGCGC TACTAGAGCT CTGGGAGCTG CGACGGCAGC AATATGAGCA GTGCATGGAC 
CTGCAGCTCT TCTACCGCGA CACGGAGCAG GTGGACAACT GGATGAGCAA GCAGGAGGCG 
TTCCTGTTGA ACGAAGACCT GGGTGATTCC TTGGATAGTG TGGAAGCGCT TCTCAAGAAG 
CACGAAGACT TTGAGAAGTC CCTCAGCGCC CAGGAGGAGA AGATCACAGC CTTAGATGAG 
TTTGCCACCA AGCTGATTCA GAACAACCAC TACGCGATGG AAGATGTGGC CACCCGGCGA 
GACGCACTGC TGAGCCGCCG CAACGCCCTC CATGAGAGAG CCATGTACCG CCGTGCCCAG 
CTTGCAGATT CCTTCCACTT GCAGCAGTTT TTCCGTGACT CTGATGAGCT CAAGAGTTGG 
GTCAACGAGA AGATGAAAAC AGCCACAGAT GAAGCTTATA AAGACCCATC CAACCTACAA 
GGGAAAGTGC AGAAGCACCA GGCCTTTGAG GCCGAGCTCT CAGCAAACCA GAGCCGCATC 
GACGCCTTGG AGAAAGCTGG GCAGAAGCTG ATCGACGTTG ACCACTATGC CAAGGATGAG 
GTGGCGACTC GGATGAATGA AGTTATCAGT CTGTGGAAGA AGCTGCTGGA GGCCACGGAG 
CTGAAAGGCA TAAAGCTCCG TGAGGCTAAC CAGCAGCAGC AGTTTAACCG CAATGTTGAG 
GACATCGAGC TGTGGCTGTA CGAAGTGGAA GGTCATCTGG CCTCGGATGA CTACGGCAAA 
GACCTCACCA ACGTCCAGAA CCTCCAGAAA AAGCATGCCC TGCTGGAGGC TGACGTGGCC 
GCTCACCAGG ATCGCATCGA TGGCATCACT ATCCAAGCCC GCCAGTTCCA GGATGCGGGC 
CACTTTGATG CTGAAAACAT CAAGAAGAAG CAGGAGGCCC TGGTAGCTCG CTACGAGGCA 
CTCAAGGAGC CCATGGTTGC CCGGAAGCAG AAGCTGGCCG ATTCCCTGCG GTTGCAGCAG 
CTCTTCCGTG ACGTTGAGGA TGAGGAGACG TGGATTCGGG AGAAAGAACC TATTGCCGCC 
TCCACCAACA GAGGCAAAGA TTTAATTGGA GTCCAGAACC TGCTAAAGAA ACACCAAGCG 
CTACAAGCAG AAATTGCTGG CCACGAGCCC CGCATCAAAG CAGTCACACA GAAGGGGAAC 
GCCATGGTAG AGGAGGGCCA TTTTGCTGCC GAGGACGTGA AGGCCAAGCT CCATGAACTG 
AACCAGAAGT GGGAGTCGCT GAAGTCCAAG GCCTCCCAGC GACGGCAGGA CCTGGAGGAC 
TCGCTGCAGG CCCAGCAGTA CTTCGCTGAT GCCAACGAGG CCGAGTCCTG GATGCGGGAG 
AAGGAGCCCA TCGTGGGCAG CACCGACTAT GGCAAAGATG AGGACTCTGC CGAGGCTCTG 
CTAAAGAAGC ACGAGGCATT GATGTCGGAT CTCAGCGCCT ATGGCAGCAG CATCCAGGCC 
CTGCGAGAGC AGGCCCAGTC GTGCCGGCAA CAAGTGGCCC CCATGGATGA CGAGACTGGG 
AAGGAGCTGG TCTTGGCGCT CTATGACTAT CAGGAGAAGA GTCCCCGAGA GGTCACCATG 
AAGAAAGGAG ACATTCTCAC CTTACTCAAC AGCACCAACA AGGACTGGTG GAAAGTGGAA 
GTGAACGACC GGCAGGGCTT CGTGCCGGCC GCCTACGTGA AGAAGCTGGA CCCAGCGCAG 
TCGGCCTCCC GGGAGAACCT CCTGGAAGAG CAGGGCAGCA TCGCGCTGCG GCAGGAGCAG 
ATTGACAACC AGTACCATTC TCTGCTGGAA CTGGGAGAGA AGCGTAAAGG CATGTTGGAG 
AAGAGCTGTA AGAAGTTCAT GCTGTTCCGT GAAGCGAACG AGCTGCAGCA GTGGATCAAC 
GAGAAGGAAG CGGCCCTGAC AAGCGAGGAG GTCGGCGCGG ACTTGGAGCA GGTCGAAGTG 
CTGCAGAAGA AGTTTGACGA CTTCCAGAAG GATCTCAAGG CCAATGAGTC ACGGCTGAAG 
GACATTAACA AGGTGGCTGA AGACCTGGAG TCGGAAGGCC TGATGGCGGA GGAGGTGCAG 
GCCGTGCAGC AGCAGGAGGT GTATGGCGCG ATGCCCAGGG ATGAATCTGA TTCCAAGACA 
GCCTCCCCGT GGAAGTCTGC TCGCCTGATG GTTCACACCG TGGCCACCTT TAATTCCATC 
AAGGAGCTCA ATGAGCGCTG GCGGTCCCTG CAGCAGCTGG CTGAGGAGCG GAGCCAACTC 
TTGGGCAGCG CCCACGAAGT GCAGCGGTTC CACAGAGATG CTGACGAAAC CAAAGAGTGG 
ATCGAGGAGA AGAACCAGGC TCTAAACACA GACAACTATG GCCACGACCT GGCCAGCGTC 
CAGGCCCTGC AGCGCAAGCA TGAGGGCTTC GAGAGGGACC TCGCGGCCCT TGGCGACAAG 
GTGAACTCGC TCGGGGAGAC AGCCGAGCGC CTGATCCAGT CCCACCCCGA GTCGGCAGAG 
GACCTGCAGG AGAAGTGCAC TGAGCTGAAC CAGGCATGGA GCAGCCTGGG CAAGCGTGCC 
GACCAGCGCA AGGCCAAGCT GGGCGACTCC CACGACCTGC AGCGCTTCCT CAGCGACTTC 
CGGGACCTCA TGTCTTGGAT CAATGGAATC CGAGGTCTGG TGTCCTCAGA CGAGCTAGCC 
AAGGATGTCA CTGGAGCTGA GGCCCTGCTG GAGCGACACC AGGAACACCG GACAGAAATT 
GATGCCAGAG CTGGCACTTT CCAGGCATTC GAGCAGTTTG GGCAGCAGCT GTTGGCTCAT 
GGGCACTACG CCAGCCCCGA GATCAAGGAG AAGCTCGATG TTCTAGACCA GGAGCGCGCA 
GACCTCGAGA AGGCCTGGGT CCAGCGCAGG ATGATGCTGG ATCAGTGCCT TGAATTGCAG 
CTGTTCCATC GGGATTGCGA GCAGGCCGAG AACTGGATGG CTGCCCGGGA GGCCTTCCTG 
AACACAGAAG ACAAAGGGGA CTCGCTAGAC AGCGTGGAGG CTCTGATCAA AAAACACGAG 
GACTTTGACA AAGCAATTAA TGTCCAGGAG GAGAAGATTG CTGCCCTGCA GTCCTTTGCT 
GACCAGCTCA TTGCTGGCGG TCACTACGCC AAAGGCGACA TCTCCAGCCG GCGCAATGAG 
GTTCTGGACA GGTGGCGCCG TCTAAAAGCT CAGATGATTG AGAAGAGGTC AAAGCTGGGA 
GAATCGCAGA CCCTCCAGCA GTTCAGCCGG GATGTAGACG AGATTGAAGC TTGGATCAGT 
GAAAAATTAC AGACTGCAAG TGATGAGTCA TATAAGGACC CCACCAACAT CCAGCTTTCC 
AAGCTGCTGA GCAAGCATCA GAAGCACCAG GCCTTCGAGG CAGAACTGCA CGCCAATGCG 
GACCGGATCC GCGGGGTCAT CGACGTGGGC AACTCCCTCA TTGACCGTGG GGCCTGTGCC 
GGCAGCGAGG ATGCCGTCAA GGCCCGGCTG GCCGCCTTAG CTGACCAGTG GCAGTTCCTG 
GTGCAGAAGT CGGCTGAGAA GAGCCAGAAG CTGAAAGAAG CCAACAAACA GCAGAACTTC 
AACACTGGCA TCAAGGACTT CGACTTCTGG CTCTCTGAGG TGGAAGCCCT GCTGGCCTCT 
GAGGATTATG GCAAAGACCT GGCTTCTGTG AACAACTTGC TAAAAAAACA TCAGCTGCTG 
GAGGCAGATA TCTCTGCCCA CGAGGACCGC CTGAAGGACC TCAACAGCCA GGCGGACAGC 
CTGATGACCA GCAGCGCCTT CGACACCTCC CAGGTGAAGG ATAAGCGGGA CACCATCAAC 
AGCCGTTTCC AGAAGATCAA GAGCATGGCC GCCTCCCGGC GAGCTAGGCT GAATGAGTCC 
CACCGCCTGC ACCAGTTCTT CCGGGACATG GACGATGAGG AGTCCTGGAT CAAGGAGAAG 
AAGCTGCTGG TGAGCTCAGA GGACTACGGC CGCGACCTGA CGGGCGTCCA GAACCTGAGG 
AAGAAGCACA AACGGCTGGA GGCAGAGCTG GCTGCCCACG AGCCGGCCAT TCAGGGTGTC 
CTGGACACTG GAAAGAAGCT GTCTGATGAC AACACCATCG GGAAGGAGGA GATTCAGCAG 
CGCCTGGCGC AGTTTGTGGA GCACTGGAAG GAGCTGAAGC AGCTGGCAGC TGCTCGGGGC 
CAGCGGCTAG AAGAATCTTT GGAATATCAG CAGTTTGTGG CCAACGTGGA GGAGGAGGAA 
GCCTGGATCA ATGAGAAGAT GACCCTGGTG GCCAGTGAGG ATTACGGAGA CACTCTTGCT 
GCCATCCAGG GCTTGCTGAA GAAACACGAA GCCTTCGAGA CGGACTTCAC TGTCCACAAG 
GACCGGGTGA ATGACGTCTG CACTAACGGA CAAGACCTGG TTAAGAAGAA CAATCACCAT 
GAGGAGAACA TCTCTTCCAA GATGAAGGGC TTGAACGGGA AAGTGTCAGA CTTGGAGAAG 
GCTGCCTCCC AGAGGAAGGC AAAGCTCGAC GAGAACTCGG CCTTCCTCCA GTTCAACTGG 
AAGGCCGATG TGGTGGAGTC CTGGATTGGT GAAAAGGAGA ACAGCTTGAA GACAGATGAT 
TATGGTCGAG ATCTGTCTTC TGTCCAAACT CTCCTCACCA AACAGGAAAC CTTCGATGCT 
GGCCTGCAGG CCTTCCAGCA GGAGGGCATC GCCAACATCA CTGCCCTCAA AGATCAGCTG 
CTGGCCGCCA AGCATGTTCA GTCCAAGGCC ATCGAGGCCC GCCACGCTGC CCTCATGAAG 
AGATGGAGCC AGCTCCTGGC CAATTCGGCC ACCCGGAAGA AGAAGCTGCT GGAGGCACAG 
AGTCACTTCC GCAAGGTGGA GGATCTCTTC CTGACCTTTG CCAAAAAGGC TTCAGCCTTC 
AACAGCTGGT TTGAAAATGC AGAGGAGGAC TTAACAGACC CAGTGCGCTG CAACTCCCTG 
GAAGAGATCA AGGCCTTGAG AGAGGCCCAC GATGCCTTCC GCTCCTCGCT GAGCTCAGCC 
CAGGCCGACT TCAACCAGCT GGCTGAGCTG GACCGCCAGA TCAAGAGCTT CCGCGTGGCC 
TCCAACCCCT ACACCTGGTT CACCATGGAG GCCCTGGAGG AGACCTGGAG GAACCTTCAG 
AAGATCATCA AGGAAAGGGA GCTGGAGCTG CAGAAGGAGC AGCGGCGGCA GGAGGAGAAC 
GACAAGCTGC GCCAGGAGTT CGCCCAGCAT GCCAACGCCT TCCACCAGTG GATCCAGGAG 
ACCAGAACGT ATCTCCTCGA TGGGTCCTGC ATGGTGGAAG AATCTGGGAC CCTTGAATCG 
CAGCTTGAAG CTACCAAACG CAAACACCAG GAGATTCGAG CCATGAGAAG TCAGCTCAAG 
AAGATTGAAG ACCTGGGGGC GGCCATGGAG GAAGCGCTGA TCCTGGACAA CAAGTACACG 
GAGCACAGCA CCGTGGGCCT GGCCCAGCAG TGGGACCAGC TCGACCAGCT GGGCATGCGG 
ATGCAGCACA ACCTGGAGCA GCAGATCCAG GCCAGGAACA CGACAGGAGT GACCGAGGAG 
GCCCTCAAAG AATTCAGCAT GATGTTCAAA CACTTCGACA AGGACAAGTC CGGCCGGCTG 
AATCACCAGG AGTTCAAATC CTGTCTGCGC TCCCTGGGCT ATGACCTGCC CATGGTGGAG 
GAAGGGGAGC CCGACCCTGA GTTCGAGGCC ATCTTGGATA CTGTGGATCC TAACAGGGAC 
GGCCACGTCT CCCTGCAAGA GTACATGGCC TTTATGATCA GCCGTGAGAC CGAGAATGTC 
AAGTCCAGCG AGGAGATTGA GAGCGCCTTC CGGGCCCTGA GCTCCGAAGG GAAGCCTTAT 
GTGACCAAGG AGGAGCTGTA CCAGAACCTG ACTCGGGAAC AAGCCGACTA CTGCGTCTCC 
CACATGAAGC CCTACGTGGA CGGCAAGGGC CGCGAACTCC CCACTGCTTT CGACTACGTT 
GAGTTCACCC GCTCGCTCTT CGTGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012002877.1
CDS	80..7489
Translation	MLSSFRKRRVQKMDPSGVKVLETAEDIQERRQQVLDRYHRFKELSTLRRQKLEDSYRFQFFQRDAEELEKWIQEKLQIASDENYKDPTNLQGKLQKHQAFEAEVQANSGAIVKLDETGNLMISEGHFASETIRTRLMELHRQWELLLEKMREKGIKLLQAQKLVQYLRECEDVMDWINDKEAIVTSEELGQDLEHVEVLQKKFEEFQTDMAAHEERVNEVNQFAAKLIQEQHPEEELIKTKEDEVNAAWQRLKGLALQRQGKLFGAAEVQRFNRDVDETISWIKEKEQLMASDDFGRDLASVQALLRKHEGLERDLAALEDKVKALCAEADRLQQSHPLSATQIQVKREELITNWEQIRTLAAERHARLNDSYRLQRFLADFRDLTSWVTEMKALINADELANDVAGAEALLDRHQEHKGEIDAHEDSFKSADESGQALLAAGHYASDEVREKLTILSEERAALLELWELRRQQYEQCMDLQLFYRDTEQVDNWMSKQEAFLLNEDLGDSLDSVEALLKKHEDFEKSLSAQEEKITALDEFATKLIQNNHYAMEDVATRRDALLSRRNALHERAMYRRAQLADSFHLQQFFRDSDELKSWVNEKMKTATDEAYKDPSNLQGKVQKHQAFEAELSANQSRIDALEKAGQKLIDVDHYAKDEVATRMNEVISLWKKLLEATELKGIKLREANQQQQFNRNVEDIELWLYEVEGHLASDDYGKDLTNVQNLQKKHALLEADVAAHQDRIDGITIQARQFQDAGHFDAENIKKKQEALVARYEALKEPMVARKQKLADSLRLQQLFRDVEDEETWIREKEPIAASTNRGKDLIGVQNLLKKHQALQAEIAGHEPRIKAVTQKGNAMVEEGHFAAEDVKAKLHELNQKWESLKSKASQRRQDLEDSLQAQQYFADANEAESWMREKEPIVGSTDYGKDEDSAEALLKKHEALMSDLSAYGSSIQALREQAQSCRQQVAPMDDETGKELVLALYDYQEKSPREVTMKKGDILTLLNSTNKDWWKVEVNDRQGFVPAAYVKKLDPAQSASRENLLEEQGSIALRQEQIDNQYHSLLELGEKRKGMLEKSCKKFMLFREANELQQWINEKEAALTSEEVGADLEQVEVLQKKFDDFQKDLKANESRLKDINKVAEDLESEGLMAEEVQAVQQQEVYGAMPRDESDSKTASPWKSARLMVHTVATFNSIKELNERWRSLQQLAEERSQLLGSAHEVQRFHRDADETKEWIEEKNQALNTDNYGHDLASVQALQRKHEGFERDLAALGDKVNSLGETAERLIQSHPESAEDLQEKCTELNQAWSSLGKRADQRKAKLGDSHDLQRFLSDFRDLMSWINGIRGLVSSDELAKDVTGAEALLERHQEHRTEIDARAGTFQAFEQFGQQLLAHGHYASPEIKEKLDVLDQERADLEKAWVQRRMMLDQCLELQLFHRDCEQAENWMAAREAFLNTEDKGDSLDSVEALIKKHEDFDKAINVQEEKIAALQSFADQLIAGGHYAKGDISSRRNEVLDRWRRLKAQMIEKRSKLGESQTLQQFSRDVDEIEAWISEKLQTASDESYKDPTNIQLSKLLSKHQKHQAFEAELHANADRIRGVIDVGNSLIDRGACAGSEDAVKARLAALADQWQFLVQKSAEKSQKLKEANKQQNFNTGIKDFDFWLSEVEALLASEDYGKDLASVNNLLKKHQLLEADISAHEDRLKDLNSQADSLMTSSAFDTSQVKDKRDTINSRFQKIKSMAASRRARLNESHRLHQFFRDMDDEESWIKEKKLLVSSEDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQFVEHWKELKQLAAARGQRLEESLEYQQFVANVEEEEAWINEKMTLVASEDYGDTLAAIQGLLKKHEAFETDFTVHKDRVNDVCTNGQDLVKKNNHHEENISSKMKGLNGKVSDLEKAASQRKAKLDENSAFLQFNWKADVVESWIGEKENSLKTDDYGRDLSSVQTLLTKQETFDAGLQAFQQEGIANITALKDQLLAAKHVQSKAIEARHAALMKRWSQLLANSATRKKKLLEAQSHFRKVEDLFLTFAKKASAFNSWFENAEEDLTDPVRCNSLEEIKALREAHDAFRSSLSSAQADFNQLAELDRQIKSFRVASNPYTWFTMEALEETWRNLQKIIKERELELQKEQRRQEENDKLRQEFAQHANAFHQWIQETRTYLLDGSCMVEESGTLESQLEATKRKHQEIRAMRSQLKKIEDLGAAMEEALILDNKYTEHSTVGLAQQWDQLDQLGMRMQHNLEQQIQARNTTGVTEEALKEFSMMFKHFDKDKSGRLNHQEFKSCLRSLGYDLPMVEEGEPDPEFEAILDTVDPNRDGHVSLQEYMAFMISRETENVKSSEEIESAFRALSSEGKPYVTKEELYQNLTREQADYCVSHMKPYVDGKGRELPTAFDYVEFTRSLFVN

Target ORF information:

RefSeq Version	XM_012147487.1
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon spectrin, alpha, non-erythrocytic 1 (SPTAN1), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012147487.1

ORF Insert Sequence:

ATGCTGTCAT CGTTTCGTAA ACGCAGAGTC CAGAAAATGG ACCCAAGCGG GGTCAAAGTG 
CTGGAGACAG CCGAGGACAT CCAGGAGAGG CGGCAGCAGG TCCTGGACAG ATACCACCGC 
TTCAAGGAGC TCTCGACCCT GAGGCGCCAG AAGCTTGAAG ACTCCTACCG ATTCCAGTTT 
TTCCAAAGAG ATGCTGAGGA GCTGGAGAAA TGGATTCAGG AGAAGCTTCA GATTGCCTCG 
GATGAGAATT ACAAAGACCC AACCAACCTG CAGGGCAAGC TGCAGAAGCA TCAAGCCTTC 
GAAGCCGAGG TGCAGGCCAA CTCGGGTGCC ATCGTGAAGC TTGATGAGAC CGGTAACCTG 
ATGATCTCAG AAGGGCACTT CGCGTCCGAA ACCATCCGGA CTCGTCTGAT GGAGCTGCAC 
CGCCAGTGGG AATTGCTTCT GGAGAAGATG CGGGAGAAAG GCATCAAGCT GCTGCAGGCC 
CAGAAGCTGG TGCAGTACCT GCGGGAGTGT GAGGACGTGA TGGACTGGAT CAATGACAAG 
GAAGCCATTG TGACATCTGA GGAGCTGGGC CAGGACCTGG AGCACGTGGA GGTGTTACAG 
AAGAAATTCG AAGAGTTTCA GACAGACATG GCTGCCCACG AAGAGAGAGT CAATGAAGTG 
AACCAGTTTG CTGCCAAGCT CATTCAGGAG CAGCACCCTG AGGAGGAGCT GATCAAGACA 
AAGGAGGACG AGGTGAATGC AGCCTGGCAG CGGCTGAAGG GCCTGGCCCT CCAGCGGCAG 
GGGAAGCTGT TCGGGGCAGC TGAGGTCCAA CGCTTTAACA GGGATGTGGA CGAGACCATT 
AGCTGGATTA AGGAGAAGGA ACAGTTAATG GCCTCTGATG ACTTTGGCCG GGACCTGGCC 
AGTGTCCAGG CTCTACTGCG GAAACACGAG GGTTTGGAGA GAGATCTTGC TGCCTTGGAG 
GACAAGGTCA AAGCCCTGTG TGCCGAGGCT GACCGCCTGC AGCAGTCCCA CCCTCTGAGT 
GCCACCCAGA TCCAAGTGAA GCGAGAGGAG CTGATCACCA ACTGGGAGCA GATCCGCACC 
CTGGCCGCGG AGAGACACGC ACGTCTCAAC GATTCCTACA GGCTTCAGCG CTTCCTTGCT 
GACTTCCGAG ACCTCACCAG CTGGGTCACT GAGATGAAAG CCCTCATCAA TGCAGATGAA 
CTTGCCAATG ATGTGGCCGG GGCCGAAGCC CTGCTGGATA GGCATCAGGA GCACAAGGGT 
GAAATTGACG CTCATGAAGA TAGCTTTAAA TCTGCAGATG AGTCTGGACA GGCCCTGCTT 
GCAGCTGGTC ACTATGCCTC AGATGAAGTG AGGGAGAAGC TGACCATCCT GTCCGAGGAG 
CGGGCCGCGC TACTAGAGCT CTGGGAGCTG CGACGGCAGC AATATGAGCA GTGCATGGAC 
CTGCAGCTCT TCTACCGCGA CACGGAGCAG GTGGACAACT GGATGAGCAA GCAGGAGGCG 
TTCCTGTTGA ACGAAGACCT GGGTGATTCC TTGGATAGTG TGGAAGCGCT TCTCAAGAAG 
CACGAAGACT TTGAGAAGTC CCTCAGCGCC CAGGAGGAGA AGATCACAGC CTTAGATGAG 
TTTGCCACCA AGCTGATTCA GAACAACCAC TACGCGATGG AAGATGTGGC CACCCGGCGA 
GACGCACTGC TGAGCCGCCG CAACGCCCTC CATGAGAGAG CCATGTACCG CCGTGCCCAG 
CTTGCAGATT CCTTCCACTT GCAGCAGTTT TTCCGTGACT CTGATGAGCT CAAGAGTTGG 
GTCAACGAGA AGATGAAAAC AGCCACAGAT GAAGCTTATA AAGACCCATC CAACCTACAA 
GGGAAAGTGC AGAAGCACCA GGCCTTTGAG GCCGAGCTCT CAGCAAACCA GAGCCGCATC 
GACGCCTTGG AGAAAGCTGG GCAGAAGCTG ATCGACGTTG ACCACTATGC CAAGGATGAG 
GTGGCGACTC GGATGAATGA AGTTATCAGT CTGTGGAAGA AGCTGCTGGA GGCCACGGAG 
CTGAAAGGCA TAAAGCTCCG TGAGGCTAAC CAGCAGCAGC AGTTTAACCG CAATGTTGAG 
GACATCGAGC TGTGGCTGTA CGAAGTGGAA GGTCATCTGG CCTCGGATGA CTACGGCAAA 
GACCTCACCA ACGTCCAGAA CCTCCAGAAA AAGCATGCCC TGCTGGAGGC TGACGTGGCC 
GCTCACCAGG ATCGCATCGA TGGCATCACT ATCCAAGCCC GCCAGTTCCA GGATGCGGGC 
CACTTTGATG CTGAAAACAT CAAGAAGAAG CAGGAGGCCC TGGTAGCTCG CTACGAGGCA 
CTCAAGGAGC CCATGGTTGC CCGGAAGCAG AAGCTGGCCG ATTCCCTGCG GTTGCAGCAG 
CTCTTCCGTG ACGTTGAGGA TGAGGAGACG TGGATTCGGG AGAAAGAACC TATTGCCGCC 
TCCACCAACA GAGGCAAAGA TTTAATTGGA GTCCAGAACC TGCTAAAGAA ACACCAAGCG 
CTACAAGCAG AAATTGCTGG CCACGAGCCC CGCATCAAAG CAGTCACACA GAAGGGGAAC 
GCCATGGTAG AGGAGGGCCA TTTTGCTGCC GAGGACGTGA AGGCCAAGCT CCATGAACTG 
AACCAGAAGT GGGAGTCGCT GAAGTCCAAG GCCTCCCAGC GACGGCAGGA CCTGGAGGAC 
TCGCTGCAGG CCCAGCAGTA CTTCGCTGAT GCCAACGAGG CCGAGTCCTG GATGCGGGAG 
AAGGAGCCCA TCGTGGGCAG CACCGACTAT GGCAAAGATG AGGACTCTGC CGAGGCTCTG 
CTAAAGAAGC ACGAGGCATT GATGTCGGAT CTCAGCGCCT ATGGCAGCAG CATCCAGGCC 
CTGCGAGAGC AGGCCCAGTC GTGCCGGCAA CAAGTGGCCC CCATGGATGA CGAGACTGGG 
AAGGAGCTGG TCTTGGCGCT CTATGACTAT CAGGAGAAGA GTCCCCGAGA GGTCACCATG 
AAGAAAGGAG ACATTCTCAC CTTACTCAAC AGCACCAACA AGGACTGGTG GAAAGTGGAA 
GTGAACGACC GGCAGGGCTT CGTGCCGGCC GCCTACGTGA AGAAGCTGGA CCCAGCGCAG 
TCGGCCTCCC GGGAGAACCT CCTGGAAGAG CAGGGCAGCA TCGCGCTGCG GCAGGAGCAG 
ATTGACAACC AGTACCATTC TCTGCTGGAA CTGGGAGAGA AGCGTAAAGG CATGTTGGAG 
AAGAGCTGTA AGAAGTTCAT GCTGTTCCGT GAAGCGAACG AGCTGCAGCA GTGGATCAAC 
GAGAAGGAAG CGGCCCTGAC AAGCGAGGAG GTCGGCGCGG ACTTGGAGCA GGTCGAAGTG 
CTGCAGAAGA AGTTTGACGA CTTCCAGAAG GATCTCAAGG CCAATGAGTC ACGGCTGAAG 
GACATTAACA AGGTGGCTGA AGACCTGGAG TCGGAAGGCC TGATGGCGGA GGAGGTGCAG 
GCCGTGCAGC AGCAGGAGGT GTATGGCGCG ATGCCCAGGG ATGAATCTGA TTCCAAGACA 
GCCTCCCCGT GGAAGTCTGC TCGCCTGATG GTTCACACCG TGGCCACCTT TAATTCCATC 
AAGGAGCTCA ATGAGCGCTG GCGGTCCCTG CAGCAGCTGG CTGAGGAGCG GAGCCAACTC 
TTGGGCAGCG CCCACGAAGT GCAGCGGTTC CACAGAGATG CTGACGAAAC CAAAGAGTGG 
ATCGAGGAGA AGAACCAGGC TCTAAACACA GACAACTATG GCCACGACCT GGCCAGCGTC 
CAGGCCCTGC AGCGCAAGCA TGAGGGCTTC GAGAGGGACC TCGCGGCCCT TGGCGACAAG 
GTGAACTCGC TCGGGGAGAC AGCCGAGCGC CTGATCCAGT CCCACCCCGA GTCGGCAGAG 
GACCTGCAGG AGAAGTGCAC TGAGCTGAAC CAGGCATGGA GCAGCCTGGG CAAGCGTGCC 
GACCAGCGCA AGGCCAAGCT GGGCGACTCC CACGACCTGC AGCGCTTCCT CAGCGACTTC 
CGGGACCTCA TGTCTTGGAT CAATGGAATC CGAGGTCTGG TGTCCTCAGA CGAGCTAGCC 
AAGGATGTCA CTGGAGCTGA GGCCCTGCTG GAGCGACACC AGGAACACCG GACAGAAATT 
GATGCCAGAG CTGGCACTTT CCAGGCATTC GAGCAGTTTG GGCAGCAGCT GTTGGCTCAT 
GGGCACTACG CCAGCCCCGA GATCAAGGAG AAGCTCGATG TTCTAGACCA GGAGCGCGCA 
GACCTCGAGA AGGCCTGGGT CCAGCGCAGG ATGATGCTGG ATCAGTGCCT TGAATTGCAG 
CTGTTCCATC GGGATTGCGA GCAGGCCGAG AACTGGATGG CTGCCCGGGA GGCCTTCCTG 
AACACAGAAG ACAAAGGGGA CTCGCTAGAC AGCGTGGAGG CTCTGATCAA AAAACACGAG 
GACTTTGACA AAGCAATTAA TGTCCAGGAG GAGAAGATTG CTGCCCTGCA GTCCTTTGCT 
GACCAGCTCA TTGCTGGCGG TCACTACGCC AAAGGCGACA TCTCCAGCCG GCGCAATGAG 
GTTCTGGACA GGTGGCGCCG TCTAAAAGCT CAGATGATTG AGAAGAGGTC AAAGCTGGGA 
GAATCGCAGA CCCTCCAGCA GTTCAGCCGG GATGTAGACG AGATTGAAGC TTGGATCAGT 
GAAAAATTAC AGACTGCAAG TGATGAGTCA TATAAGGACC CCACCAACAT CCAGCTTTCC 
AAGCTGCTGA GCAAGCATCA GAAGCACCAG GCCTTCGAGG CAGAACTGCA CGCCAATGCG 
GACCGGATCC GCGGGGTCAT CGACGTGGGC AACTCCCTCA TTGACCGTGG GGCCTGTGCC 
GGCAGCGAGG ATGCCGTCAA GGCCCGGCTG GCCGCCTTAG CTGACCAGTG GCAGTTCCTG 
GTGCAGAAGT CGGCTGAGAA GAGCCAGAAG CTGAAAGAAG CCAACAAACA GCAGAACTTC 
AACACTGGCA TCAAGGACTT CGACTTCTGG CTCTCTGAGG TGGAAGCCCT GCTGGCCTCT 
GAGGATTATG GCAAAGACCT GGCTTCTGTG AACAACTTGC TAAAAAAACA TCAGCTGCTG 
GAGGCAGATA TCTCTGCCCA CGAGGACCGC CTGAAGGACC TCAACAGCCA GGCGGACAGC 
CTGATGACCA GCAGCGCCTT CGACACCTCC CAGGTGAAGG ATAAGCGGGA CACCATCAAC 
AGCCGTTTCC AGAAGATCAA GAGCATGGCC GCCTCCCGGC GAGCTAGGCT GAATGAGTCC 
CACCGCCTGC ACCAGTTCTT CCGGGACATG GACGATGAGG AGTCCTGGAT CAAGGAGAAG 
AAGCTGCTGG TGAGCTCAGA GGACTACGGC CGCGACCTGA CGGGCGTCCA GAACCTGAGG 
AAGAAGCACA AACGGCTGGA GGCAGAGCTG GCTGCCCACG AGCCGGCCAT TCAGGGTGTC 
CTGGACACTG GAAAGAAGCT GTCTGATGAC AACACCATCG GGAAGGAGGA GATTCAGCAG 
CGCCTGGCGC AGTTTGTGGA GCACTGGAAG GAGCTGAAGC AGCTGGCAGC TGCTCGGGGC 
CAGCGGCTAG AAGAATCTTT GGAATATCAG CAGTTTGTGG CCAACGTGGA GGAGGAGGAA 
GCCTGGATCA ATGAGAAGAT GACCCTGGTG GCCAGTGAGG ATTACGGAGA CACTCTTGCT 
GCCATCCAGG GCTTGCTGAA GAAACACGAA GCCTTCGAGA CGGACTTCAC TGTCCACAAG 
GACCGGGTGA ATGACGTCTG CACTAACGGA CAAGACCTGG TTAAGAAGAA CAATCACCAT 
GAGGAGAACA TCTCTTCCAA GATGAAGGGC TTGAACGGGA AAGTGTCAGA CTTGGAGAAG 
GCTGCCTCCC AGAGGAAGGC AAAGCTCGAC GAGAACTCGG CCTTCCTCCA GTTCAACTGG 
AAGGCCGATG TGGTGGAGTC CTGGATTGGT GAAAAGGAGA ACAGCTTGAA GACAGATGAT 
TATGGTCGAG ATCTGTCTTC TGTCCAAACT CTCCTCACCA AACAGGAAAC CTTCGATGCT 
GGCCTGCAGG CCTTCCAGCA GGAGGGCATC GCCAACATCA CTGCCCTCAA AGATCAGCTG 
CTGGCCGCCA AGCATGTTCA GTCCAAGGCC ATCGAGGCCC GCCACGCTGC CCTCATGAAG 
AGATGGAGCC AGCTCCTGGC CAATTCGGCC ACCCGGAAGA AGAAGCTGCT GGAGGCACAG 
AGTCACTTCC GCAAGGTGGA GGATCTCTTC CTGACCTTTG CCAAAAAGGC TTCAGCCTTC 
AACAGCTGGT TTGAAAATGC AGAGGAGGAC TTAACAGACC CAGTGCGCTG CAACTCCCTG 
GAAGAGATCA AGGCCTTGAG AGAGGCCCAC GATGCCTTCC GCTCCTCGCT GAGCTCAGCC 
CAGGCCGACT TCAACCAGCT GGCTGAGCTG GACCGCCAGA TCAAGAGCTT CCGCGTGGCC 
TCCAACCCCT ACACCTGGTT CACCATGGAG GCCCTGGAGG AGACCTGGAG GAACCTTCAG 
AAGATCATCA AGGAAAGGGA GCTGGAGCTG CAGAAGGAGC AGCGGCGGCA GGAGGAGAAC 
GACAAGCTGC GCCAGGAGTT CGCCCAGCAT GCCAACGCCT TCCACCAGTG GATCCAGGAG 
ACCAGAACGT ATCTCCTCGA TGGGTCCTGC ATGGTGGAAG AATCTGGGAC CCTTGAATCG 
CAGCTTGAAG CTACCAAACG CAAACACCAG GAGATTCGAG CCATGAGAAG TCAGCTCAAG 
AAGATTGAAG ACCTGGGGGC GGCCATGGAG GAAGCGCTGA TCCTGGACAA CAAGTACACG 
GAGCACAGCA CCGTGGGCCT GGCCCAGCAG TGGGACCAGC TCGACCAGCT GGGCATGCGG 
ATGCAGCACA ACCTGGAGCA GCAGATCCAG GCCAGGAACA CGACAGGAGT GACCGAGGAG 
GCCCTCAAAG AATTCAGCAT GATGTTCAAA CACTTCGACA AGGACAAGTC CGGCCGGCTG 
AATCACCAGG AGTTCAAATC CTGTCTGCGC TCCCTGGGCT ATGACCTGCC CATGGTGGAG 
GAAGGGGAGC CCGACCCTGA GTTCGAGGCC ATCTTGGATA CTGTGGATCC TAACAGGGAC 
GGCCACGTCT CCCTGCAAGA GTACATGGCC TTTATGATCA GCCGTGAGAC CGAGAATGTC 
AAGTCCAGCG AGGAGATTGA GAGCGCCTTC CGGGCCCTGA GCTCCGAAGG GAAGCCTTAT 
GTGACCAAGG AGGAGCTGTA CCAGAACCTG ACTCGGGAAC AAGCCGACTA CTGCGTCTCC 
CACATGAAGC CCTACGTGGA CGGCAAGGGC CGCGAACTCC CCACTGCTTT CGACTACGTT 
GAGTTCACCC GCTCGCTCTT CGTGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTAN1 ( XM_012147488.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_012002878.1 Ovis aries musimon spectrin, alpha, non-erythrocytic 1 (SPTAN1), transcript variant X12, mRNA.

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CloneID	OOv24458
Clone ID Related Accession (Same CDS sequence)	XM_012147488.1
Accession Version	XM_012147488.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7395bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	spectrin alpha chain, non-erythrocytic 1 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942583.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTCAT CGTTTCGTAA ACGCAGAGTC CAGAAAATGG ACCCAAGCGG GGTCAAAGTG 
CTGGAGACAG CCGAGGACAT CCAGGAGAGG CGGCAGCAGG TCCTGGACAG ATACCACCGC 
TTCAAGGAGC TCTCGACCCT GAGGCGCCAG AAGCTTGAAG ACTCCTACCG ATTCCAGTTT 
TTCCAAAGAG ATGCTGAGGA GCTGGAGAAA TGGATTCAGG AGAAGCTTCA GATTGCCTCG 
GATGAGAATT ACAAAGACCC AACCAACCTG CAGGGCAAGC TGCAGAAGCA TCAAGCCTTC 
GAAGCCGAGG TGCAGGCCAA CTCGGGTGCC ATCGTGAAGC TTGATGAGAC CGGTAACCTG 
ATGATCTCAG AAGGGCACTT CGCGTCCGAA ACCATCCGGA CTCGTCTGAT GGAGCTGCAC 
CGCCAGTGGG AATTGCTTCT GGAGAAGATG CGGGAGAAAG GCATCAAGCT GCTGCAGGCC 
CAGAAGCTGG TGCAGTACCT GCGGGAGTGT GAGGACGTGA TGGACTGGAT CAATGACAAG 
GAAGCCATTG TGACATCTGA GGAGCTGGGC CAGGACCTGG AGCACGTGGA GGTGTTACAG 
AAGAAATTCG AAGAGTTTCA GACAGACATG GCTGCCCACG AAGAGAGAGT CAATGAAGTG 
AACCAGTTTG CTGCCAAGCT CATTCAGGAG CAGCACCCTG AGGAGGAGCT GATCAAGACA 
AAGGAGGACG AGGTGAATGC AGCCTGGCAG CGGCTGAAGG GCCTGGCCCT CCAGCGGCAG 
GGGAAGCTGT TCGGGGCAGC TGAGGTCCAA CGCTTTAACA GGGATGTGGA CGAGACCATT 
AGCTGGATTA AGGAGAAGGA ACAGTTAATG GCCTCTGATG ACTTTGGCCG GGACCTGGCC 
AGTGTCCAGG CTCTACTGCG GAAACACGAG GGTTTGGAGA GAGATCTTGC TGCCTTGGAG 
GACAAGGTCA AAGCCCTGTG TGCCGAGGCT GACCGCCTGC AGCAGTCCCA CCCTCTGAGT 
GCCACCCAGA TCCAAGTGAA GCGAGAGGAG CTGATCACCA ACTGGGAGCA GATCCGCACC 
CTGGCCGCGG AGAGACACGC ACGTCTCAAC GATTCCTACA GGCTTCAGCG CTTCCTTGCT 
GACTTCCGAG ACCTCACCAG CTGGGTCACT GAGATGAAAG CCCTCATCAA TGCAGATGAA 
CTTGCCAATG ATGTGGCCGG GGCCGAAGCC CTGCTGGATA GGCATCAGGA GCACAAGGGT 
GAAATTGACG CTCATGAAGA TAGCTTTAAA TCTGCAGATG AGTCTGGACA GGCCCTGCTT 
GCAGCTGGTC ACTATGCCTC AGATGAAGTG AGGGAGAAGC TGACCATCCT GTCCGAGGAG 
CGGGCCGCGC TACTAGAGCT CTGGGAGCTG CGACGGCAGC AATATGAGCA GTGCATGGAC 
CTGCAGCTCT TCTACCGCGA CACGGAGCAG GTGGACAACT GGATGAGCAA GCAGGAGGCG 
TTCCTGTTGA ACGAAGACCT GGGTGATTCC TTGGATAGTG TGGAAGCGCT TCTCAAGAAG 
CACGAAGACT TTGAGAAGTC CCTCAGCGCC CAGGAGGAGA AGATCACAGC CTTAGATGAG 
TTTGCCACCA AGCTGATTCA GAACAACCAC TACGCGATGG AAGATGTGGC CACCCGGCGA 
GACGCACTGC TGAGCCGCCG CAACGCCCTC CATGAGAGAG CCATGTACCG CCGTGCCCAG 
CTTGCAGATT CCTTCCACTT GCAGCAGTTT TTCCGTGACT CTGATGAGCT CAAGAGTTGG 
GTCAACGAGA AGATGAAAAC AGCCACAGAT GAAGCTTATA AAGACCCATC CAACCTACAA 
GGGAAAGTGC AGAAGCACCA GGCCTTTGAG GCCGAGCTCT CAGCAAACCA GAGCCGCATC 
GACGCCTTGG AGAAAGCTGG GCAGAAGCTG ATCGACGTTG ACCACTATGC CAAGGATGAG 
GTGGCGACTC GGATGAATGA AGTTATCAGT CTGTGGAAGA AGCTGCTGGA GGCCACGGAG 
CTGAAAGGCA TAAAGCTCCG TGAGGCTAAC CAGCAGCAGC AGTTTAACCG CAATGTTGAG 
GACATCGAGC TGTGGCTGTA CGAAGTGGAA GGTCATCTGG CCTCGGATGA CTACGGCAAA 
GACCTCACCA ACGTCCAGAA CCTCCAGAAA AAGCATGCCC TGCTGGAGGC TGACGTGGCC 
GCTCACCAGG ATCGCATCGA TGGCATCACT ATCCAAGCCC GCCAGTTCCA GGATGCGGGC 
CACTTTGATG CTGAAAACAT CAAGAAGAAG CAGGAGGCCC TGGTAGCTCG CTACGAGGCA 
CTCAAGGAGC CCATGGTTGC CCGGAAGCAG AAGCTGGCCG ATTCCCTGCG GTTGCAGCAG 
CTCTTCCGTG ACGTTGAGGA TGAGGAGACG TGGATTCGGG AGAAAGAACC TATTGCCGCC 
TCCACCAACA GAGGCAAAGA TTTAATTGGA GTCCAGAACC TGCTAAAGAA ACACCAAGCG 
CTACAAGCAG AAATTGCTGG CCACGAGCCC CGCATCAAAG CAGTCACACA GAAGGGGAAC 
GCCATGGTAG AGGAGGGCCA TTTTGCTGCC GAGGACGTGA AGGCCAAGCT CCATGAACTG 
AACCAGAAGT GGGAGTCGCT GAAGTCCAAG GCCTCCCAGC GACGGCAGGA CCTGGAGGAC 
TCGCTGCAGG CCCAGCAGTA CTTCGCTGAT GCCAACGAGG CCGAGTCCTG GATGCGGGAG 
AAGGAGCCCA TCGTGGGCAG CACCGACTAT GGCAAAGATG AGGACTCTGC CGAGGCTCTG 
CTAAAGAAGC ACGAGGCATT GATGTCGGAT CTCAGCGCCT ATGGCAGCAG CATCCAGGCC 
CTGCGAGAGC AGGCCCAGTC GTGCCGGCAA CAAGTGGCCC CCATGGATGA CGAGACTGGG 
AAGGAGCTGG TCTTGGCGCT CTATGACTAT CAGGAGAAGA GTCCCCGAGA GGTCACCATG 
AAGAAAGGAG ACATTCTCAC CTTACTCAAC AGCACCAACA AGGACTGGTG GAAAGTGGAA 
GTGAACGACC GGCAGGGCTT CGTGCCGGCC GCCTACGTGA AGAAGCTGGA CCCAGCGCAG 
TCGGCCTCCC GGGAGAACCT CCTGGAAGAG CAGGGCAGCA TCGCGCTGCG GCAGGAGCAG 
ATTGACAACC AGTACCATTC TCTGCTGGAA CTGGGAGAGA AGCGTAAAGG CATGTTGGAG 
AAGAGCTGTA AGAAGTTCAT GCTGTTCCGT GAAGCGAACG AGCTGCAGCA GTGGATCAAC 
GAGAAGGAAG CGGCCCTGAC AAGCGAGGAG GTCGGCGCGG ACTTGGAGCA GGTCGAAGTG 
CTGCAGAAGA AGTTTGACGA CTTCCAGAAG GATCTCAAGG CCAATGAGTC ACGGCTGAAG 
GACATTAACA AGGTGGCTGA AGACCTGGAG TCGGAAGGCC TGATGGCGGA GGAGGTGCAG 
GCCGTGCAGC AGCAGGAGGT GTATGGCGCG ATGCCCAGGG ATGAATCTGA TTCCAAGACA 
GCCTCCCCGT GGAAGTCTGC TCGCCTGATG GTTCACACCG TGGCCACCTT TAATTCCATC 
AAGGAGCTCA ATGAGCGCTG GCGGTCCCTG CAGCAGCTGG CTGAGGAGCG GAGCCAACTC 
TTGGGCAGCG CCCACGAAGT GCAGCGGTTC CACAGAGATG CTGACGAAAC CAAAGAGTGG 
ATCGAGGAGA AGAACCAGGC TCTAAACACA GACAACTATG GCCACGACCT GGCCAGCGTC 
CAGGCCCTGC AGCGCAAGCA TGAGGGCTTC GAGAGGGACC TCGCGGCCCT TGGCGACAAG 
GTGAACTCGC TCGGGGAGAC AGCCGAGCGC CTGATCCAGT CCCACCCCGA GTCGGCAGAG 
GACCTGCAGG AGAAGTGCAC TGAGCTGAAC CAGGCATGGA GCAGCCTGGG CAAGCGTGCC 
GACCAGCGCA AGGCCAAGCT GGGCGACTCC CACGACCTGC AGCGCTTCCT CAGCGACTTC 
CGGGACCTCA TGTCTTGGAT CAATGGAATC CGAGGTCTGG TGTCCTCAGA CGAGCTAGCC 
AAGGATGTCA CTGGAGCTGA GGCCCTGCTG GAGCGACACC AGGAACACCG GACAGAAATT 
GATGCCAGAG CTGGCACTTT CCAGGCATTC GAGCAGTTTG GGCAGCAGCT GTTGGCTCAT 
GGGCACTACG CCAGCCCCGA GATCAAGGAG AAGCTCGATG TTCTAGACCA GGAGCGCGCA 
GACCTCGAGA AGGCCTGGGT CCAGCGCAGG ATGATGCTGG ATCAGTGCCT TGAATTGCAG 
CTGTTCCATC GGGATTGCGA GCAGGCCGAG AACTGGATGG CTGCCCGGGA GGCCTTCCTG 
AACACAGAAG ACAAAGGGGA CTCGCTAGAC AGCGTGGAGG CTCTGATCAA AAAACACGAG 
GACTTTGACA AAGCAATTAA TGTCCAGGAG GAGAAGATTG CTGCCCTGCA GTCCTTTGCT 
GACCAGCTCA TTGCTGGCGG TCACTACGCC AAAGGCGACA TCTCCAGCCG GCGCAATGAG 
GTTCTGGACA GGTGGCGCCG TCTAAAAGCT CAGATGATTG AGAAGAGGTC AAAGCTGGGA 
GAATCGCAGA CCCTCCAGCA GTTCAGCCGG GATGTAGACG AGATTGAAGC TTGGATCAGT 
GAAAAATTAC AGACTGCAAG TGATGAGTCA TATAAGGACC CCACCAACAT CCAGAGCAAG 
CATCAGAAGC ACCAGGCCTT CGAGGCAGAA CTGCACGCCA ATGCGGACCG GATCCGCGGG 
GTCATCGACG TGGGCAACTC CCTCATTGAC CGTGGGGCCT GTGCCGGCAG CGAGGATGCC 
GTCAAGGCCC GGCTGGCCGC CTTAGCTGAC CAGTGGCAGT TCCTGGTGCA GAAGTCGGCT 
GAGAAGAGCC AGAAGCTGAA AGAAGCCAAC AAACAGCAGA ACTTCAACAC TGGCATCAAG 
GACTTCGACT TCTGGCTCTC TGAGGTGGAA GCCCTGCTGG CCTCTGAGGA TTATGGCAAA 
GACCTGGCTT CTGTGAACAA CTTGCTAAAA AAACATCAGC TGCTGGAGGC AGATATCTCT 
GCCCACGAGG ACCGCCTGAA GGACCTCAAC AGCCAGGCGG ACAGCCTGAT GACCAGCAGC 
GCCTTCGACA CCTCCCAGGT GAAGGATAAG CGGGACACCA TCAACAGCCG TTTCCAGAAG 
ATCAAGAGCA TGGCCGCCTC CCGGCGAGCT AGGCTGAATG AGTCCCACCG CCTGCACCAG 
TTCTTCCGGG ACATGGACGA TGAGGAGTCC TGGATCAAGG AGAAGAAGCT GCTGGTGAGC 
TCAGAGGACT ACGGCCGCGA CCTGACGGGC GTCCAGAACC TGAGGAAGAA GCACAAACGG 
CTGGAGGCAG AGCTGGCTGC CCACGAGCCG GCCATTCAGG GTGTCCTGGA CACTGGAAAG 
AAGCTGTCTG ATGACAACAC CATCGGGAAG GAGGAGATTC AGCAGCGCCT GGCGCAGTTT 
GTGGAGCACT GGAAGGAGCT GAAGCAGCTG GCAGCTGCTC GGGGCCAGCG GCTAGAAGAA 
TCTTTGGAAT ATCAGCAGTT TGTGGCCAAC GTGGAGGAGG AGGAAGCCTG GATCAATGAG 
AAGATGACCC TGGTGGCCAG TGAGGATTAC GGAGACACTC TTGCTGCCAT CCAGGGCTTG 
CTGAAGAAAC ACGAAGCCTT CGAGACGGAC TTCACTGTCC ACAAGGACCG GGTGAATGAC 
GTCTGCACTA ACGGACAAGA CCTGGTTAAG AAGAACAATC ACCATGAGGA GAACATCTCT 
TCCAAGATGA AGGGCTTGAA CGGGAAAGTG TCAGACTTGG AGAAGGCTGC CTCCCAGAGG 
AAGGCAAAGC TCGACGAGAA CTCGGCCTTC CTCCAGTTCA ACTGGAAGGC CGATGTGGTG 
GAGTCCTGGA TTGGTGAAAA GGAGAACAGC TTGAAGACAG ATGATTATGG TCGAGATCTG 
TCTTCTGTCC AAACTCTCCT CACCAAACAG GAAACCTTCG ATGCTGGCCT GCAGGCCTTC 
CAGCAGGAGG GCATCGCCAA CATCACTGCC CTCAAAGATC AGCTGCTGGC CGCCAAGCAT 
GTTCAGTCCA AGGCCATCGA GGCCCGCCAC GCTGCCCTCA TGAAGAGATG GAGCCAGCTC 
CTGGCCAATT CGGCCACCCG GAAGAAGAAG CTGCTGGAGG CACAGAGTCA CTTCCGCAAG 
GTGGAGGATC TCTTCCTGAC CTTTGCCAAA AAGGCTTCAG CCTTCAACAG CTGGTTTGAA 
AATGCAGAGG AGGACTTAAC AGACCCAGTG CGCTGCAACT CCCTGGAAGA GATCAAGGCC 
TTGAGAGAGG CCCACGATGC CTTCCGCTCC TCGCTGAGCT CAGCCCAGGC CGACTTCAAC 
CAGCTGGCTG AGCTGGACCG CCAGATCAAG AGCTTCCGCG TGGCCTCCAA CCCCTACACC 
TGGTTCACCA TGGAGGCCCT GGAGGAGACC TGGAGGAACC TTCAGAAGAT CATCAAGGAA 
AGGGAGCTGG AGCTGCAGAA GGAGCAGCGG CGGCAGGAGG AGAACGACAA GCTGCGCCAG 
GAGTTCGCCC AGCATGCCAA CGCCTTCCAC CAGTGGATCC AGGAGACCAG AACGTATCTC 
CTCGATGGGT CCTGCATGGT GGAAGAATCT GGGACCCTTG AATCGCAGCT TGAAGCTACC 
AAACGCAAAC ACCAGGAGAT TCGAGCCATG AGAAGTCAGC TCAAGAAGAT TGAAGACCTG 
GGGGCGGCCA TGGAGGAAGC GCTGATCCTG GACAACAAGT ACACGGAGCA CAGCACCGTG 
GGCCTGGCCC AGCAGTGGGA CCAGCTCGAC CAGCTGGGCA TGCGGATGCA GCACAACCTG 
GAGCAGCAGA TCCAGGCCAG GAACACGACA GGAGTGACCG AGGAGGCCCT CAAAGAATTC 
AGCATGATGT TCAAACACTT CGACAAGGAC AAGTCCGGCC GGCTGAATCA CCAGGAGTTC 
AAATCCTGTC TGCGCTCCCT GGGCTATGAC CTGCCCATGG TGGAGGAAGG GGAGCCCGAC 
CCTGAGTTCG AGGCCATCTT GGATACTGTG GATCCTAACA GGGACGGCCA CGTCTCCCTG 
CAAGAGTACA TGGCCTTTAT GATCAGCCGT GAGACCGAGA ATGTCAAGTC CAGCGAGGAG 
ATTGAGAGCG CCTTCCGGGC CCTGAGCTCC GAAGGGAAGC CTTATGTGAC CAAGGAGGAG 
CTGTACCAGA ACCTGACTCG GGAACAAGCC GACTACTGCG TCTCCCACAT GAAGCCCTAC 
GTGGACGGCA AGGGCCGCGA ACTCCCCACT GCTTTCGACT ACGTTGAGTT CACCCGCTCG 
CTCTTCGTGA ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012002878.1
CDS	80..7474
Translation	MLSSFRKRRVQKMDPSGVKVLETAEDIQERRQQVLDRYHRFKELSTLRRQKLEDSYRFQFFQRDAEELEKWIQEKLQIASDENYKDPTNLQGKLQKHQAFEAEVQANSGAIVKLDETGNLMISEGHFASETIRTRLMELHRQWELLLEKMREKGIKLLQAQKLVQYLRECEDVMDWINDKEAIVTSEELGQDLEHVEVLQKKFEEFQTDMAAHEERVNEVNQFAAKLIQEQHPEEELIKTKEDEVNAAWQRLKGLALQRQGKLFGAAEVQRFNRDVDETISWIKEKEQLMASDDFGRDLASVQALLRKHEGLERDLAALEDKVKALCAEADRLQQSHPLSATQIQVKREELITNWEQIRTLAAERHARLNDSYRLQRFLADFRDLTSWVTEMKALINADELANDVAGAEALLDRHQEHKGEIDAHEDSFKSADESGQALLAAGHYASDEVREKLTILSEERAALLELWELRRQQYEQCMDLQLFYRDTEQVDNWMSKQEAFLLNEDLGDSLDSVEALLKKHEDFEKSLSAQEEKITALDEFATKLIQNNHYAMEDVATRRDALLSRRNALHERAMYRRAQLADSFHLQQFFRDSDELKSWVNEKMKTATDEAYKDPSNLQGKVQKHQAFEAELSANQSRIDALEKAGQKLIDVDHYAKDEVATRMNEVISLWKKLLEATELKGIKLREANQQQQFNRNVEDIELWLYEVEGHLASDDYGKDLTNVQNLQKKHALLEADVAAHQDRIDGITIQARQFQDAGHFDAENIKKKQEALVARYEALKEPMVARKQKLADSLRLQQLFRDVEDEETWIREKEPIAASTNRGKDLIGVQNLLKKHQALQAEIAGHEPRIKAVTQKGNAMVEEGHFAAEDVKAKLHELNQKWESLKSKASQRRQDLEDSLQAQQYFADANEAESWMREKEPIVGSTDYGKDEDSAEALLKKHEALMSDLSAYGSSIQALREQAQSCRQQVAPMDDETGKELVLALYDYQEKSPREVTMKKGDILTLLNSTNKDWWKVEVNDRQGFVPAAYVKKLDPAQSASRENLLEEQGSIALRQEQIDNQYHSLLELGEKRKGMLEKSCKKFMLFREANELQQWINEKEAALTSEEVGADLEQVEVLQKKFDDFQKDLKANESRLKDINKVAEDLESEGLMAEEVQAVQQQEVYGAMPRDESDSKTASPWKSARLMVHTVATFNSIKELNERWRSLQQLAEERSQLLGSAHEVQRFHRDADETKEWIEEKNQALNTDNYGHDLASVQALQRKHEGFERDLAALGDKVNSLGETAERLIQSHPESAEDLQEKCTELNQAWSSLGKRADQRKAKLGDSHDLQRFLSDFRDLMSWINGIRGLVSSDELAKDVTGAEALLERHQEHRTEIDARAGTFQAFEQFGQQLLAHGHYASPEIKEKLDVLDQERADLEKAWVQRRMMLDQCLELQLFHRDCEQAENWMAAREAFLNTEDKGDSLDSVEALIKKHEDFDKAINVQEEKIAALQSFADQLIAGGHYAKGDISSRRNEVLDRWRRLKAQMIEKRSKLGESQTLQQFSRDVDEIEAWISEKLQTASDESYKDPTNIQSKHQKHQAFEAELHANADRIRGVIDVGNSLIDRGACAGSEDAVKARLAALADQWQFLVQKSAEKSQKLKEANKQQNFNTGIKDFDFWLSEVEALLASEDYGKDLASVNNLLKKHQLLEADISAHEDRLKDLNSQADSLMTSSAFDTSQVKDKRDTINSRFQKIKSMAASRRARLNESHRLHQFFRDMDDEESWIKEKKLLVSSEDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQFVEHWKELKQLAAARGQRLEESLEYQQFVANVEEEEAWINEKMTLVASEDYGDTLAAIQGLLKKHEAFETDFTVHKDRVNDVCTNGQDLVKKNNHHEENISSKMKGLNGKVSDLEKAASQRKAKLDENSAFLQFNWKADVVESWIGEKENSLKTDDYGRDLSSVQTLLTKQETFDAGLQAFQQEGIANITALKDQLLAAKHVQSKAIEARHAALMKRWSQLLANSATRKKKLLEAQSHFRKVEDLFLTFAKKASAFNSWFENAEEDLTDPVRCNSLEEIKALREAHDAFRSSLSSAQADFNQLAELDRQIKSFRVASNPYTWFTMEALEETWRNLQKIIKERELELQKEQRRQEENDKLRQEFAQHANAFHQWIQETRTYLLDGSCMVEESGTLESQLEATKRKHQEIRAMRSQLKKIEDLGAAMEEALILDNKYTEHSTVGLAQQWDQLDQLGMRMQHNLEQQIQARNTTGVTEEALKEFSMMFKHFDKDKSGRLNHQEFKSCLRSLGYDLPMVEEGEPDPEFEAILDTVDPNRDGHVSLQEYMAFMISRETENVKSSEEIESAFRALSSEGKPYVTKEELYQNLTREQADYCVSHMKPYVDGKGRELPTAFDYVEFTRSLFVN

Target ORF information:

RefSeq Version	XM_012147488.1
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon spectrin, alpha, non-erythrocytic 1 (SPTAN1), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012147488.1

ORF Insert Sequence:

ATGCTGTCAT CGTTTCGTAA ACGCAGAGTC CAGAAAATGG ACCCAAGCGG GGTCAAAGTG 
CTGGAGACAG CCGAGGACAT CCAGGAGAGG CGGCAGCAGG TCCTGGACAG ATACCACCGC 
TTCAAGGAGC TCTCGACCCT GAGGCGCCAG AAGCTTGAAG ACTCCTACCG ATTCCAGTTT 
TTCCAAAGAG ATGCTGAGGA GCTGGAGAAA TGGATTCAGG AGAAGCTTCA GATTGCCTCG 
GATGAGAATT ACAAAGACCC AACCAACCTG CAGGGCAAGC TGCAGAAGCA TCAAGCCTTC 
GAAGCCGAGG TGCAGGCCAA CTCGGGTGCC ATCGTGAAGC TTGATGAGAC CGGTAACCTG 
ATGATCTCAG AAGGGCACTT CGCGTCCGAA ACCATCCGGA CTCGTCTGAT GGAGCTGCAC 
CGCCAGTGGG AATTGCTTCT GGAGAAGATG CGGGAGAAAG GCATCAAGCT GCTGCAGGCC 
CAGAAGCTGG TGCAGTACCT GCGGGAGTGT GAGGACGTGA TGGACTGGAT CAATGACAAG 
GAAGCCATTG TGACATCTGA GGAGCTGGGC CAGGACCTGG AGCACGTGGA GGTGTTACAG 
AAGAAATTCG AAGAGTTTCA GACAGACATG GCTGCCCACG AAGAGAGAGT CAATGAAGTG 
AACCAGTTTG CTGCCAAGCT CATTCAGGAG CAGCACCCTG AGGAGGAGCT GATCAAGACA 
AAGGAGGACG AGGTGAATGC AGCCTGGCAG CGGCTGAAGG GCCTGGCCCT CCAGCGGCAG 
GGGAAGCTGT TCGGGGCAGC TGAGGTCCAA CGCTTTAACA GGGATGTGGA CGAGACCATT 
AGCTGGATTA AGGAGAAGGA ACAGTTAATG GCCTCTGATG ACTTTGGCCG GGACCTGGCC 
AGTGTCCAGG CTCTACTGCG GAAACACGAG GGTTTGGAGA GAGATCTTGC TGCCTTGGAG 
GACAAGGTCA AAGCCCTGTG TGCCGAGGCT GACCGCCTGC AGCAGTCCCA CCCTCTGAGT 
GCCACCCAGA TCCAAGTGAA GCGAGAGGAG CTGATCACCA ACTGGGAGCA GATCCGCACC 
CTGGCCGCGG AGAGACACGC ACGTCTCAAC GATTCCTACA GGCTTCAGCG CTTCCTTGCT 
GACTTCCGAG ACCTCACCAG CTGGGTCACT GAGATGAAAG CCCTCATCAA TGCAGATGAA 
CTTGCCAATG ATGTGGCCGG GGCCGAAGCC CTGCTGGATA GGCATCAGGA GCACAAGGGT 
GAAATTGACG CTCATGAAGA TAGCTTTAAA TCTGCAGATG AGTCTGGACA GGCCCTGCTT 
GCAGCTGGTC ACTATGCCTC AGATGAAGTG AGGGAGAAGC TGACCATCCT GTCCGAGGAG 
CGGGCCGCGC TACTAGAGCT CTGGGAGCTG CGACGGCAGC AATATGAGCA GTGCATGGAC 
CTGCAGCTCT TCTACCGCGA CACGGAGCAG GTGGACAACT GGATGAGCAA GCAGGAGGCG 
TTCCTGTTGA ACGAAGACCT GGGTGATTCC TTGGATAGTG TGGAAGCGCT TCTCAAGAAG 
CACGAAGACT TTGAGAAGTC CCTCAGCGCC CAGGAGGAGA AGATCACAGC CTTAGATGAG 
TTTGCCACCA AGCTGATTCA GAACAACCAC TACGCGATGG AAGATGTGGC CACCCGGCGA 
GACGCACTGC TGAGCCGCCG CAACGCCCTC CATGAGAGAG CCATGTACCG CCGTGCCCAG 
CTTGCAGATT CCTTCCACTT GCAGCAGTTT TTCCGTGACT CTGATGAGCT CAAGAGTTGG 
GTCAACGAGA AGATGAAAAC AGCCACAGAT GAAGCTTATA AAGACCCATC CAACCTACAA 
GGGAAAGTGC AGAAGCACCA GGCCTTTGAG GCCGAGCTCT CAGCAAACCA GAGCCGCATC 
GACGCCTTGG AGAAAGCTGG GCAGAAGCTG ATCGACGTTG ACCACTATGC CAAGGATGAG 
GTGGCGACTC GGATGAATGA AGTTATCAGT CTGTGGAAGA AGCTGCTGGA GGCCACGGAG 
CTGAAAGGCA TAAAGCTCCG TGAGGCTAAC CAGCAGCAGC AGTTTAACCG CAATGTTGAG 
GACATCGAGC TGTGGCTGTA CGAAGTGGAA GGTCATCTGG CCTCGGATGA CTACGGCAAA 
GACCTCACCA ACGTCCAGAA CCTCCAGAAA AAGCATGCCC TGCTGGAGGC TGACGTGGCC 
GCTCACCAGG ATCGCATCGA TGGCATCACT ATCCAAGCCC GCCAGTTCCA GGATGCGGGC 
CACTTTGATG CTGAAAACAT CAAGAAGAAG CAGGAGGCCC TGGTAGCTCG CTACGAGGCA 
CTCAAGGAGC CCATGGTTGC CCGGAAGCAG AAGCTGGCCG ATTCCCTGCG GTTGCAGCAG 
CTCTTCCGTG ACGTTGAGGA TGAGGAGACG TGGATTCGGG AGAAAGAACC TATTGCCGCC 
TCCACCAACA GAGGCAAAGA TTTAATTGGA GTCCAGAACC TGCTAAAGAA ACACCAAGCG 
CTACAAGCAG AAATTGCTGG CCACGAGCCC CGCATCAAAG CAGTCACACA GAAGGGGAAC 
GCCATGGTAG AGGAGGGCCA TTTTGCTGCC GAGGACGTGA AGGCCAAGCT CCATGAACTG 
AACCAGAAGT GGGAGTCGCT GAAGTCCAAG GCCTCCCAGC GACGGCAGGA CCTGGAGGAC 
TCGCTGCAGG CCCAGCAGTA CTTCGCTGAT GCCAACGAGG CCGAGTCCTG GATGCGGGAG 
AAGGAGCCCA TCGTGGGCAG CACCGACTAT GGCAAAGATG AGGACTCTGC CGAGGCTCTG 
CTAAAGAAGC ACGAGGCATT GATGTCGGAT CTCAGCGCCT ATGGCAGCAG CATCCAGGCC 
CTGCGAGAGC AGGCCCAGTC GTGCCGGCAA CAAGTGGCCC CCATGGATGA CGAGACTGGG 
AAGGAGCTGG TCTTGGCGCT CTATGACTAT CAGGAGAAGA GTCCCCGAGA GGTCACCATG 
AAGAAAGGAG ACATTCTCAC CTTACTCAAC AGCACCAACA AGGACTGGTG GAAAGTGGAA 
GTGAACGACC GGCAGGGCTT CGTGCCGGCC GCCTACGTGA AGAAGCTGGA CCCAGCGCAG 
TCGGCCTCCC GGGAGAACCT CCTGGAAGAG CAGGGCAGCA TCGCGCTGCG GCAGGAGCAG 
ATTGACAACC AGTACCATTC TCTGCTGGAA CTGGGAGAGA AGCGTAAAGG CATGTTGGAG 
AAGAGCTGTA AGAAGTTCAT GCTGTTCCGT GAAGCGAACG AGCTGCAGCA GTGGATCAAC 
GAGAAGGAAG CGGCCCTGAC AAGCGAGGAG GTCGGCGCGG ACTTGGAGCA GGTCGAAGTG 
CTGCAGAAGA AGTTTGACGA CTTCCAGAAG GATCTCAAGG CCAATGAGTC ACGGCTGAAG 
GACATTAACA AGGTGGCTGA AGACCTGGAG TCGGAAGGCC TGATGGCGGA GGAGGTGCAG 
GCCGTGCAGC AGCAGGAGGT GTATGGCGCG ATGCCCAGGG ATGAATCTGA TTCCAAGACA 
GCCTCCCCGT GGAAGTCTGC TCGCCTGATG GTTCACACCG TGGCCACCTT TAATTCCATC 
AAGGAGCTCA ATGAGCGCTG GCGGTCCCTG CAGCAGCTGG CTGAGGAGCG GAGCCAACTC 
TTGGGCAGCG CCCACGAAGT GCAGCGGTTC CACAGAGATG CTGACGAAAC CAAAGAGTGG 
ATCGAGGAGA AGAACCAGGC TCTAAACACA GACAACTATG GCCACGACCT GGCCAGCGTC 
CAGGCCCTGC AGCGCAAGCA TGAGGGCTTC GAGAGGGACC TCGCGGCCCT TGGCGACAAG 
GTGAACTCGC TCGGGGAGAC AGCCGAGCGC CTGATCCAGT CCCACCCCGA GTCGGCAGAG 
GACCTGCAGG AGAAGTGCAC TGAGCTGAAC CAGGCATGGA GCAGCCTGGG CAAGCGTGCC 
GACCAGCGCA AGGCCAAGCT GGGCGACTCC CACGACCTGC AGCGCTTCCT CAGCGACTTC 
CGGGACCTCA TGTCTTGGAT CAATGGAATC CGAGGTCTGG TGTCCTCAGA CGAGCTAGCC 
AAGGATGTCA CTGGAGCTGA GGCCCTGCTG GAGCGACACC AGGAACACCG GACAGAAATT 
GATGCCAGAG CTGGCACTTT CCAGGCATTC GAGCAGTTTG GGCAGCAGCT GTTGGCTCAT 
GGGCACTACG CCAGCCCCGA GATCAAGGAG AAGCTCGATG TTCTAGACCA GGAGCGCGCA 
GACCTCGAGA AGGCCTGGGT CCAGCGCAGG ATGATGCTGG ATCAGTGCCT TGAATTGCAG 
CTGTTCCATC GGGATTGCGA GCAGGCCGAG AACTGGATGG CTGCCCGGGA GGCCTTCCTG 
AACACAGAAG ACAAAGGGGA CTCGCTAGAC AGCGTGGAGG CTCTGATCAA AAAACACGAG 
GACTTTGACA AAGCAATTAA TGTCCAGGAG GAGAAGATTG CTGCCCTGCA GTCCTTTGCT 
GACCAGCTCA TTGCTGGCGG TCACTACGCC AAAGGCGACA TCTCCAGCCG GCGCAATGAG 
GTTCTGGACA GGTGGCGCCG TCTAAAAGCT CAGATGATTG AGAAGAGGTC AAAGCTGGGA 
GAATCGCAGA CCCTCCAGCA GTTCAGCCGG GATGTAGACG AGATTGAAGC TTGGATCAGT 
GAAAAATTAC AGACTGCAAG TGATGAGTCA TATAAGGACC CCACCAACAT CCAGAGCAAG 
CATCAGAAGC ACCAGGCCTT CGAGGCAGAA CTGCACGCCA ATGCGGACCG GATCCGCGGG 
GTCATCGACG TGGGCAACTC CCTCATTGAC CGTGGGGCCT GTGCCGGCAG CGAGGATGCC 
GTCAAGGCCC GGCTGGCCGC CTTAGCTGAC CAGTGGCAGT TCCTGGTGCA GAAGTCGGCT 
GAGAAGAGCC AGAAGCTGAA AGAAGCCAAC AAACAGCAGA ACTTCAACAC TGGCATCAAG 
GACTTCGACT TCTGGCTCTC TGAGGTGGAA GCCCTGCTGG CCTCTGAGGA TTATGGCAAA 
GACCTGGCTT CTGTGAACAA CTTGCTAAAA AAACATCAGC TGCTGGAGGC AGATATCTCT 
GCCCACGAGG ACCGCCTGAA GGACCTCAAC AGCCAGGCGG ACAGCCTGAT GACCAGCAGC 
GCCTTCGACA CCTCCCAGGT GAAGGATAAG CGGGACACCA TCAACAGCCG TTTCCAGAAG 
ATCAAGAGCA TGGCCGCCTC CCGGCGAGCT AGGCTGAATG AGTCCCACCG CCTGCACCAG 
TTCTTCCGGG ACATGGACGA TGAGGAGTCC TGGATCAAGG AGAAGAAGCT GCTGGTGAGC 
TCAGAGGACT ACGGCCGCGA CCTGACGGGC GTCCAGAACC TGAGGAAGAA GCACAAACGG 
CTGGAGGCAG AGCTGGCTGC CCACGAGCCG GCCATTCAGG GTGTCCTGGA CACTGGAAAG 
AAGCTGTCTG ATGACAACAC CATCGGGAAG GAGGAGATTC AGCAGCGCCT GGCGCAGTTT 
GTGGAGCACT GGAAGGAGCT GAAGCAGCTG GCAGCTGCTC GGGGCCAGCG GCTAGAAGAA 
TCTTTGGAAT ATCAGCAGTT TGTGGCCAAC GTGGAGGAGG AGGAAGCCTG GATCAATGAG 
AAGATGACCC TGGTGGCCAG TGAGGATTAC GGAGACACTC TTGCTGCCAT CCAGGGCTTG 
CTGAAGAAAC ACGAAGCCTT CGAGACGGAC TTCACTGTCC ACAAGGACCG GGTGAATGAC 
GTCTGCACTA ACGGACAAGA CCTGGTTAAG AAGAACAATC ACCATGAGGA GAACATCTCT 
TCCAAGATGA AGGGCTTGAA CGGGAAAGTG TCAGACTTGG AGAAGGCTGC CTCCCAGAGG 
AAGGCAAAGC TCGACGAGAA CTCGGCCTTC CTCCAGTTCA ACTGGAAGGC CGATGTGGTG 
GAGTCCTGGA TTGGTGAAAA GGAGAACAGC TTGAAGACAG ATGATTATGG TCGAGATCTG 
TCTTCTGTCC AAACTCTCCT CACCAAACAG GAAACCTTCG ATGCTGGCCT GCAGGCCTTC 
CAGCAGGAGG GCATCGCCAA CATCACTGCC CTCAAAGATC AGCTGCTGGC CGCCAAGCAT 
GTTCAGTCCA AGGCCATCGA GGCCCGCCAC GCTGCCCTCA TGAAGAGATG GAGCCAGCTC 
CTGGCCAATT CGGCCACCCG GAAGAAGAAG CTGCTGGAGG CACAGAGTCA CTTCCGCAAG 
GTGGAGGATC TCTTCCTGAC CTTTGCCAAA AAGGCTTCAG CCTTCAACAG CTGGTTTGAA 
AATGCAGAGG AGGACTTAAC AGACCCAGTG CGCTGCAACT CCCTGGAAGA GATCAAGGCC 
TTGAGAGAGG CCCACGATGC CTTCCGCTCC TCGCTGAGCT CAGCCCAGGC CGACTTCAAC 
CAGCTGGCTG AGCTGGACCG CCAGATCAAG AGCTTCCGCG TGGCCTCCAA CCCCTACACC 
TGGTTCACCA TGGAGGCCCT GGAGGAGACC TGGAGGAACC TTCAGAAGAT CATCAAGGAA 
AGGGAGCTGG AGCTGCAGAA GGAGCAGCGG CGGCAGGAGG AGAACGACAA GCTGCGCCAG 
GAGTTCGCCC AGCATGCCAA CGCCTTCCAC CAGTGGATCC AGGAGACCAG AACGTATCTC 
CTCGATGGGT CCTGCATGGT GGAAGAATCT GGGACCCTTG AATCGCAGCT TGAAGCTACC 
AAACGCAAAC ACCAGGAGAT TCGAGCCATG AGAAGTCAGC TCAAGAAGAT TGAAGACCTG 
GGGGCGGCCA TGGAGGAAGC GCTGATCCTG GACAACAAGT ACACGGAGCA CAGCACCGTG 
GGCCTGGCCC AGCAGTGGGA CCAGCTCGAC CAGCTGGGCA TGCGGATGCA GCACAACCTG 
GAGCAGCAGA TCCAGGCCAG GAACACGACA GGAGTGACCG AGGAGGCCCT CAAAGAATTC 
AGCATGATGT TCAAACACTT CGACAAGGAC AAGTCCGGCC GGCTGAATCA CCAGGAGTTC 
AAATCCTGTC TGCGCTCCCT GGGCTATGAC CTGCCCATGG TGGAGGAAGG GGAGCCCGAC 
CCTGAGTTCG AGGCCATCTT GGATACTGTG GATCCTAACA GGGACGGCCA CGTCTCCCTG 
CAAGAGTACA TGGCCTTTAT GATCAGCCGT GAGACCGAGA ATGTCAAGTC CAGCGAGGAG 
ATTGAGAGCG CCTTCCGGGC CCTGAGCTCC GAAGGGAAGC CTTATGTGAC CAAGGAGGAG 
CTGTACCAGA ACCTGACTCG GGAACAAGCC GACTACTGCG TCTCCCACAT GAAGCCCTAC 
GTGGACGGCA AGGGCCGCGA ACTCCCCACT GCTTTCGACT ACGTTGAGTT CACCCGCTCG 
CTCTTCGTGA ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTAN1 ( XM_015093852.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_014949338.1 Ovis aries spectrin, alpha, non-erythrocytic 1 (SPTAN1), transcript variant X1, mRNA.

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CloneID	OSm48298
Clone ID Related Accession (Same CDS sequence)	XM_015093852.1
Accession Version	XM_015093852.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7197bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries(sheep)
Product	spectrin alpha chain, non-erythrocytic 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014639012.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 3% of CDS bases ##RefSeq-Attributes-END##

ATGCTGTCAT CGTTTCGTAA ACGCAGAGTC CAGAAAATGG ACCCAAGCGG GGTCAAAGTG 
CTGGAGACAG CCGAGGACAT CCAGGAGAGG CGGCAGCAGG TCCTGGACAG ATACCACCGC 
TTCAAGGAGC TCTCGACCCT GAGGCGCCAG AAGCTTGAAG ACTCCTACCG ATTCCAGTTT 
TTCCAAAGAG ATGCTGAGGA GCTGGAGAAA TGGATTCAGG AGAAGCTTCA GATTGCCTCG 
GATGAGAATT ACAAAGACCC AACCAACCTG CAGGGCAAGC TGCAGAAGCA TCAAGCCTTC 
GAAGCCGAGG TGCAGGCCAA CTCGGGTGCC ATCGTGAAGC TTGATGAGAC CGGTAACCTG 
ATGATCTCAG AAGGGCACTT CGCGTCCGAA ACCATCCGGA CTCGTCTGAT GGAGCTGCAC 
CGCCAGTGGG AATTGCTTCT GGAGAAGATG CGGGAGAAAG GCATCAAGCT GCTGCAGGCC 
CAGAAGCTGG TGCAGTACCT GCGGGAGTGT GAGGACGTGA TGGACTGGAT CAATGACAAG 
GAAGCCATTG TGACATCTGA GGAGCTGGGC CAGGACCTGG AGCACGTGGA GGTGTTACAG 
AAGAAATTCG AAGAGTTTCA GACAGACATG GCTGCCCACG AAGAGAGAGT CAATGAAGTG 
AACCAGTTTG CTGCCAAGCT CATTCAGGAG CAGCACCCTG AGGAGGAGCT GATCAAGACA 
AAGGAGGACG AGGTGAATGC AGCCTGGCAG CGGCTGAAGG GCCTGGCCCT CCAGCGGCAG 
GGGAAGCTGT TCGGGGCAGC TGAGGTCCAA CGCTTTAACA GGGATGTGGA CGAGACCATT 
AGCTGGATTA AGGAGAAGGA ACAGTTAATG GCCTCTGATG ACTTTGGCCG GGACCTGGCC 
AGTGTCCAGG CTCTACTGCG GAAACACGAG GGTTTGGAGA GAGATCTTGC TGCCTTGGAG 
GACAAGGTCA AAGCCCTGTG TGCCGAGGCT GACCGCCTGC AGCAGTCCCA CCCTCTGAGT 
GCCACCCAGA TCCAAGTGAA GCGAGAGGAG CTGATCACCA ACTGGGAGCA GATCCGCACC 
CTGGCCGCGG AGAGACACGC ACGTCTCAAC GATTCCTACA GGCTTCAGCG CTTCCTTGCT 
GACTTCCGAG ACCTCACCAG CTGGGTCACT GAGATGAAAG CCCTCATCAA TGCAGATGAA 
CTTGCCAATG ATGTGGCCGG GGCCGAAGCC CTGCTGGATA GGCATCAGGA GCACAAGGGT 
GAAATTGACG CTCATGAAGA TAGCTTTAAA TCTGCAGATG AGTCTGGACA GGCCCTGCTT 
GCAGCTGGTC ACTATGCCTC AGATGAAGTG AGGGAGAAGC TGACCATCCT GTCCGAGGAG 
CGGGCCGCGC TACTAGAGCT CTGGGAGCTG CGACGGCAGC AATATGAGCA GTGCATGGAC 
CTGCAGCTCT TCTACCGCGA CACGGAGCAG GTGGACAACT GGATGAGCAA ACAGGAGGCG 
TTCCTGTTGA ACGAAGACCT GGGTGATTCC TTGGATAGTG TGGAAGCGCT TCTCAAGAAG 
CACGAAGACT TTGAGAAGTC CCTCAGCGCC CAGGAGGAGA AGATCACAGC CTTAGATGAG 
TTTGCCACCA AGCTGATTCA GAACAACCAC TACGCGATGG AAGATGTGGC CACCCGGCGA 
GACGCACTGC TGAGCCGCCG CAACGCCCTC CATGAGAGAG CCATGTACCG CCGTGCCCAG 
CTTGCAGATT CCTTCCACTT GCAGCAGTTT TTCCGTGACT CTGATGAGCT CAAGAGTTGG 
GTCAACGAGA AGATGAAAAC AGCCACAGAT GAAGCTTATA AAGACCCATC CAACCTACAA 
GGGAAAGTGC AGAAGCACCA GGCCTTTGAG GCCGAGCTCT CAGCAAACCA GAGCCGCATC 
GACGCCTTGG AGAAAGCTGG GCAGAAGCTG ATCGACGTTG ACCACTATGC CAAGGATGAG 
GTGGCGACTC GGATGAATGA AGTTATCAGT CTGTGGAAGA AGCTGCTGGA GGCCACGGAG 
CTGAAAGGCA TAAAGCTCCG TGAGGCTAAC CAGCAGCAGC AGTTTAACCG CAACGTTGAG 
GACATCGAGC TGTGGCTGTA CGAAGTGGAA GGTCATCTGG CCTCGGATGA CTACGGCAAA 
GACCTCACCA ACGTCCAGAA CCTCCAGAAA AAGCATGCCC TGCTGGAGGC TGACGTGGCC 
GCTCACCAGG ATCGCATCGA TGGCATCACT ATCCAAGCCC GCCAGTTCCA GGATGCGGGC 
CACTTTGATG CTGAAAACAT CAAGAAGAAG CAGGAGGCCC TGGTAGCTCG CTACGAGGCA 
CTCAAGGAGC CCATGGTTGC CCGGAAGCAG AAGCTGGCCG ATTCCCTGCG GTTGCAGCAG 
CTCTTCCGTG ACGTTGAGGA TGAGGAGACG TGGATTCGGG AGAAAGAACC TATTGCCGCC 
TCCACCAACA GAGGCAAAGA TTTAATTGGA GTCCAGAACC TGCTAAAGAA ACACCAAGCG 
CTACAAGCAG AAATTGCTGG CCACGAGCCC CGCATCAAAG CAGTCACACA GAAGGGGAAC 
GCCATGGTAG AGGAGGGCCA TTTTGCTGCC GAGGACGTGA AGGCCAAGCT CCATGAACTG 
AACCAGAAGT GGGAGTCGCT GAAGTCCAAA GCCTCCCAGC GACGGCAGGA CCTGGAGGAC 
TCGCTGCAGG CCCAGCAGTA CTTCGCTGAT GCCAACGAGG CCGAGTCCTG GATGCGGGAG 
AAGGAGCCCA TCGTGGGCAG CACCGACTAT GGCAAAGATG AGGACTCTGC CGAGGCTCTG 
CTAAAGAAGC ACGAGGCATT GATGTCGGAT CTCAGCGCCT ATGGCAGCAG CATCCAGGCC 
CTGCGAGAGC AGGCCCAGTC GTGCCGGCAA CAAGTGGCCC CCATGGATGA CGAGACTGGG 
AAGGAGCTGG TCTTGGCGCT CTATGACTAT CAGGAGAAGA GTCCCCGAGA GGTCACCATG 
AAGAAAGGAG ACATTCTCAC CTTACTCAAC AGCACCAACA AGGACTGGTG GAAAGTGGAA 
GTGAACGACC GGCAGGGCTT CGTGCCGGCC GCCTACGTGA AGAAGCTGGA CCCAGCGCAG 
TCGGCCTCCC GGGAGAACCT CCTGGAAGAG CAGGGCAGCA TCGCGCTGCG GCAGGAGCAG 
ATTGACAACC AGACGCGCGT AACTAAGGAG GCCGGCAGCG TGTCTCTGCG TATGAAACAG 
GTGGAAGAAC TGTACCATTC TCTGCTGGAA CTGGGAGAGA AGCGTAAAGG CATGTTGGAG 
AAGAGCTGTA AGAAGTTCAT GCTGTTCCGT GAAGCGAACG AGCTGCAGCA GTGGATCAAC 
GAGAAGGAAG CGGCCCTGAC AAGCGAGGAG GTCGGCGCGG ACTTGGAGCA GGTTGAAGTG 
CTGCAGAAGA AGTTTGACGA CTTCCAGAAG GATCTCAAGG CCAATGAGTC ACGGCTGAAG 
GACATTAACA AGGTGGCTGA AGACCTGGAG TCGGAAGGCC TGATGGCGGA GGAGGTGCAG 
GCCGTGCAGC AGCAGGAGGT GTATGGCGCG ATGCCCAGGG ATGAATCTGA TTCCAAGACA 
GCCTCCCCGT GGAAGTCTGC TCGCCTGATG GTTCACACCG TGGCCACCTT TAATTCCATC 
AAGGAGCTCA ATGAGCGCTG GCGGTCCCTG CAGCAGCTGG CTGAGGAGCG GAGCCAACTC 
TTGGGCAGCG CCCACGAAGT GCAGCGGTTC CACAGAGATG CTGACGAAAC CAAAGAGTGG 
ATCGAGGAGA AGAACCAGGC TCTAAACACA GACAACTATG GCCACGACCT GGCCAGCGTC 
CAGGCCCTGC AGCGCAAGCA TGAGGGCTTC GAGAGGGACC TCGCGGCCCT TGGCGACAAG 
GTGAACTCGC TCGGGGAGAC AGCCGAGCGC CTGATCCAGT CCCACCCCGA GTCGGCAGAG 
GACCTGCAGG AGAAGTGCAC TGAGCTGAAC CAGGCATGGA GCAGCCTGGG CAAGCGTGCC 
GACCAGCGCA AGGCCAAGCT GGGCGACTCC CACGACCTGC AGCGCTTCCT CAGCGACTTC 
CGGGACCTCA TGTCTTGGAT CAATGGAATC CGAGGTCTGG TGTCCTCAGA CGAGCTAGCC 
AAGGATGTCA CTGGAGCTGA GGCCCTGCTG GAGCGACACC AGGAACACCG GACAGAAATT 
GATGCCAGAG CTGGCACTTT CCAGGCATTC GAGCAGTTTG GGCAGCAGCT GTTGGCTCAT 
GGGCACTACG CCAGCCCCGA GATCAAGGAG AAGCTCGATG TTCTAGACCA GGAGCGCGCA 
GACCTCGAGA AGGCCTGGGT CCAGCGCAGG ATGATGCTGG ATCAGTGCCT TGAATTGCAG 
CTGTTCCATC GGGATTGCGA GCAGGCCGAG AACTGGATGG CTGCCCGGGA GGCCTTCCTG 
AACACAGAAG ACAAAGGGGA CTCGCTAGAC AGCGTGGAGG CTCTGATCAA AAAACACGAG 
GACTTTGACA AAGCAATTAA TGTCCAGGAG GAGAAGATTG CTGCCCTGCA GTCCTTTGCT 
GACCAGCTCA TTGCTGGCGG TCACTACGCC AAAGGCGACA TCTCCAGCCG GCGCAATGAG 
GTTCTGGACA GGTGGCGCCG TCTAAAAGCT CAGATGATTG AGAAGAGGTC AAAGCTGGGA 
GAATCGCAGA CCCTCCAGCA GTTCAGCCGG GATGTAGACG AGATTGAAGC TTGGATCAGT 
GAAAAATTAC AGACTGCAAG TGATGAGTCA TATAAGGACC CCACCAACAT CCAGCTTTCC 
AAGCTGCTGA GCAAGCATCA GAAGCACCAG GCCTTCGAGG CAGAACTGCA CGCCAATGCG 
GACCGGATCC GCGGGGTCAT CGACGTGGGC AACTCCCTCA TTGACCGTGG GGCCTGTGCC 
GGCAGCGAGG ATGCCGTCAA GGCCCGGCTG GCCGCCTTAG CTGACCAGTG GCAGTTCCTG 
GTGCAGAAGT CGGCTGAGAA GAGCCAGAAG CTGAAAGAAG CCAACAAACA GCAGAACTTC 
AACACTGGCA TCAAGGACTT CGACTTCTGG CTCTCTGAGG TGGAAGCCCT GCTGGCCTCT 
GAGGATTATG GCAAAGACCT GGCTTCTGTG AACAACTTGC TAAAAAAACA TCAGCTGCTG 
GAGGCAGATA TCTCTGCCCA CGAGGACCGC CTGAAGGACC TCAACAGCCA GGCGGACAGC 
CTGATGACCA GCAGCGCCTT CGACACCTCC CAGGTGAAGG ATAAGCGGGA CACCATCAAC 
AGCCGTTTCC AGAAGATCAA GAGCATGGCC GCCTCCCGGC GAGCTAGGCT GAATGAGTCC 
CACCGCCTGC ACCAGTTCTT CCGGGACATG GACGATGAGG AGTCCTGGAT CAAGGAGAAG 
AAGCTGCTGG TGAGCTCAGA GGACTACGGC CGCGACCTGA CGGGCGTCCA GAACCTGAGG 
AAGAAGCACA AACGGCTGGA GGCAGAGCTG GCTGCCCACG AGCCGGCCAT TCAGGGTGTC 
CTGGACACTG GAAAGAAGCT GTCTGATGAC AACACCATCG GGAAGGAGGA GATTCAGCAG 
CGCCTGGCGC AGTTTGTGGA GCACTGGAAG GAGCTGAAGC AGCTGGCAGC TGCTCGGGGC 
CAGCGGCTAG AAGAATCTTT GGAATATCAG CAGTTTGTGG CCAACGTGGA GGAGGAGGAA 
GCCTGGATCA ATGAGAAGAT GACCCTGGTG GCCAGTGAGG ATTACGGAGA CACTCTTGCT 
GCCATCCAGG GCTTGCTGAA GAAACACGAA GCCTTCGAGA CGGACTTCAC TGTCCACAAG 
GACCGGGTGA ATGACGTCTG CACTAACGGA CAAGACCTGG TTAAGAAGAA CAATCACCAT 
GAGGAGAACA TCTCTTCCAA GATGAAGGGC TTGAACGGGA AAGTGTCAGA CTTGGAGAAG 
GCTGCCTCCC AGAGGAAGGC AAAGCTCGAC GAGAACTCGG CCTTCCTCCA GTTCAACTGG 
AAGGCCGATG TGGTGGAGTC CTGGATTGGT GAAAAGGAGA ACAGCTTGAA GACAGATGAT 
TATGGTCGAG ATCTGTCTTC TGTCCAAACT CTCCTCACCA AACAGGAAAC CTTCGATGCT 
GGCCTGCAGG CCTTCCAGCA GGAGGGCATC GCCAACATCA CTGCCCTCAA AGATCAGCTG 
CTGGCCGCCA AGCATGTTCA GTCCAAGGCC ATCGAGGCCC GCCACGCTGC CCTCATGAAG 
AGATGGAGCC AGCTCCTGGC CAATTCGGCC ACCCGGAAGA AGAAGCTGCT GGAGGCACAG 
AGTCACTTCC GCAAGGTGGA GGATCTCTTC CTGACCTTTG CCAAAAAGGC TTCAGCCTTC 
AACAGCTGGT TTGAAAATGC AGAGGAGGAC TTAACAGACC CAGTGCGCTG CAACTCCCTG 
GAAGAGATCA AGGCCTTGAG AGAGGCCCAC GATGCCTTCC GCTCCTCGCT GAGCTCAGCC 
CAGGCCGACT TCAACCAGCT GGCTGAGCTG GACCGCCAGA TCAAGAGCTT CCGCGTGGCC 
TCCAACCCCT ACACCTGGTT CACCATGGAG GCCCTGGAGG AGACCTGGAG GAACCTTCAG 
AAGATCATCA AGGAAAGGGA GCTGGAGCTG CAGAAGGAGC AGCGGCGGCA GGAGGAGAAC 
GACAAGCTGC GCCAGGAGTT CGCCCAGCAT GCCAACGCCT TCCACCAGTG GATCCAGGAG 
ACCAGAACGT ATCTCCTCGA TGGCATAGCG TATCGTCGGG TCATTCGTGT CTATCAGTAT 
GAAGTTGGGG ATGATCTGTC TGGAAGGTCC TGCATGTGGA AGAATCTGGG ACCCTTGAAT 
CGCAGCTTGA AGCTACCAAA CGCAAACACC AGGAGATTCG AGCGCATGCA GAAGTCAGCT 
CAGAAGATGA AGGACCGCTG GGGCGGCCAT GGAGGAAGCG CTGATCCTGG ACAACAAGAG 
ACACGGAGCA CGAGCACCGT GGGCCTGGCC CAGCAGTGGG ACCAGCGTCG ACCAGCTAGG 
GCATGCGGAT GCAGCACAAC CTCGGGAGCA GCAGATCCAG GCCAGAACCT GACTGTGGGA 
ACAAGCCGAC TACTGCGTCT CCACATGAAG CCTTACGTGG ACGCAAGGGC CGCGAACTCG 
CCACTGGCTT GCATTGACTA CGTTGGGAGT TCACCCGCTC GCTCTTCGGT GAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014949338.1
CDS	1843..9039
Translation	MLSSFRKRRVQKMDPSGVKVLETAEDIQERRQQVLDRYHRFKELSTLRRQKLEDSYRFQFFQRDAEELEKWIQEKLQIASDENYKDPTNLQGKLQKHQAFEAEVQANSGAIVKLDETGNLMISEGHFASETIRTRLMELHRQWELLLEKMREKGIKLLQAQKLVQYLRECEDVMDWINDKEAIVTSEELGQDLEHVEVLQKKFEEFQTDMAAHEERVNEVNQFAAKLIQEQHPEEELIKTKEDEVNAAWQRLKGLALQRQGKLFGAAEVQRFNRDVDETISWIKEKEQLMASDDFGRDLASVQALLRKHEGLERDLAALEDKVKALCAEADRLQQSHPLSATQIQVKREELITNWEQIRTLAAERHARLNDSYRLQRFLADFRDLTSWVTEMKALINADELANDVAGAEALLDRHQEHKGEIDAHEDSFKSADESGQALLAAGHYASDEVREKLTILSEERAALLELWELRRQQYEQCMDLQLFYRDTEQVDNWMSKQEAFLLNEDLGDSLDSVEALLKKHEDFEKSLSAQEEKITALDEFATKLIQNNHYAMEDVATRRDALLSRRNALHERAMYRRAQLADSFHLQQFFRDSDELKSWVNEKMKTATDEAYKDPSNLQGKVQKHQAFEAELSANQSRIDALEKAGQKLIDVDHYAKDEVATRMNEVISLWKKLLEATELKGIKLREANQQQQFNRNVEDIELWLYEVEGHLASDDYGKDLTNVQNLQKKHALLEADVAAHQDRIDGITIQARQFQDAGHFDAENIKKKQEALVARYEALKEPMVARKQKLADSLRLQQLFRDVEDEETWIREKEPIAASTNRGKDLIGVQNLLKKHQALQAEIAGHEPRIKAVTQKGNAMVEEGHFAAEDVKAKLHELNQKWESLKSKASQRRQDLEDSLQAQQYFADANEAESWMREKEPIVGSTDYGKDEDSAEALLKKHEALMSDLSAYGSSIQALREQAQSCRQQVAPMDDETGKELVLALYDYQEKSPREVTMKKGDILTLLNSTNKDWWKVEVNDRQGFVPAAYVKKLDPAQSASRENLLEEQGSIALRQEQIDNQTRVTKEAGSVSLRMKQVEELYHSLLELGEKRKGMLEKSCKKFMLFREANELQQWINEKEAALTSEEVGADLEQVEVLQKKFDDFQKDLKANESRLKDINKVAEDLESEGLMAEEVQAVQQQEVYGAMPRDESDSKTASPWKSARLMVHTVATFNSIKELNERWRSLQQLAEERSQLLGSAHEVQRFHRDADETKEWIEEKNQALNTDNYGHDLASVQALQRKHEGFERDLAALGDKVNSLGETAERLIQSHPESAEDLQEKCTELNQAWSSLGKRADQRKAKLGDSHDLQRFLSDFRDLMSWINGIRGLVSSDELAKDVTGAEALLERHQEHRTEIDARAGTFQAFEQFGQQLLAHGHYASPEIKEKLDVLDQERADLEKAWVQRRMMLDQCLELQLFHRDCEQAENWMAAREAFLNTEDKGDSLDSVEALIKKHEDFDKAINVQEEKIAALQSFADQLIAGGHYAKGDISSRRNEVLDRWRRLKAQMIEKRSKLGESQTLQQFSRDVDEIEAWISEKLQTASDESYKDPTNIQLSKLLSKHQKHQAFEAELHANADRIRGVIDVGNSLIDRGACAGSEDAVKARLAALADQWQFLVQKSAEKSQKLKEANKQQNFNTGIKDFDFWLSEVEALLASEDYGKDLASVNNLLKKHQLLEADISAHEDRLKDLNSQADSLMTSSAFDTSQVKDKRDTINSRFQKIKSMAASRRARLNESHRLHQFFRDMDDEESWIKEKKLLVSSEDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQFVEHWKELKQLAAARGQRLEESLEYQQFVANVEEEEAWINEKMTLVASEDYGDTLAAIQGLLKKHEAFETDFTVHKDRVNDVCTNGQDLVKKNNHHEENISSKMKGLNGKVSDLEKAASQRKAKLDENSAFLQFNWKADVVESWIGEKENSLKTDDYGRDLSSVQTLLTKQETFDAGLQAFQQEGIANITALKDQLLAAKHVQSKAIEARHAALMKRWSQLLANSATRKKKLLEAQSHFRKVEDLFLTFAKKASAFNSWFENAEEDLTDPVRCNSLEEIKALREAHDAFRSSLSSAQADFNQLAELDRQIKSFRVASNPYTWFTMEALEETWRNLQKIIKERELELQKEQRRQEENDKLRQEFAQHANAFHQWIQETRTYLLDGIAYRRVIRVYQYEVGDDLSGRSCMWKNLGPLNRSLKLPNANTRRFERMQKSAQKMKDRWGGHGGSADPGQQETRSTSTVGLAQQWDQRRPARACGCSTTSGAADPGQNLTVGTSRLLRLHMKPYVDARAANSPLACIDYVGSSPARSSVN

Target ORF information:

RefSeq Version	XM_015093852.1
Organism	Ovis aries(sheep)
Definition	Ovis aries spectrin, alpha, non-erythrocytic 1 (SPTAN1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015093852.1

ORF Insert Sequence:

ATGCTGTCAT CGTTTCGTAA ACGCAGAGTC CAGAAAATGG ACCCAAGCGG GGTCAAAGTG 
CTGGAGACAG CCGAGGACAT CCAGGAGAGG CGGCAGCAGG TCCTGGACAG ATACCACCGC 
TTCAAGGAGC TCTCGACCCT GAGGCGCCAG AAGCTTGAAG ACTCCTACCG ATTCCAGTTT 
TTCCAAAGAG ATGCTGAGGA GCTGGAGAAA TGGATTCAGG AGAAGCTTCA GATTGCCTCG 
GATGAGAATT ACAAAGACCC AACCAACCTG CAGGGCAAGC TGCAGAAGCA TCAAGCCTTC 
GAAGCCGAGG TGCAGGCCAA CTCGGGTGCC ATCGTGAAGC TTGATGAGAC CGGTAACCTG 
ATGATCTCAG AAGGGCACTT CGCGTCCGAA ACCATCCGGA CTCGTCTGAT GGAGCTGCAC 
CGCCAGTGGG AATTGCTTCT GGAGAAGATG CGGGAGAAAG GCATCAAGCT GCTGCAGGCC 
CAGAAGCTGG TGCAGTACCT GCGGGAGTGT GAGGACGTGA TGGACTGGAT CAATGACAAG 
GAAGCCATTG TGACATCTGA GGAGCTGGGC CAGGACCTGG AGCACGTGGA GGTGTTACAG 
AAGAAATTCG AAGAGTTTCA GACAGACATG GCTGCCCACG AAGAGAGAGT CAATGAAGTG 
AACCAGTTTG CTGCCAAGCT CATTCAGGAG CAGCACCCTG AGGAGGAGCT GATCAAGACA 
AAGGAGGACG AGGTGAATGC AGCCTGGCAG CGGCTGAAGG GCCTGGCCCT CCAGCGGCAG 
GGGAAGCTGT TCGGGGCAGC TGAGGTCCAA CGCTTTAACA GGGATGTGGA CGAGACCATT 
AGCTGGATTA AGGAGAAGGA ACAGTTAATG GCCTCTGATG ACTTTGGCCG GGACCTGGCC 
AGTGTCCAGG CTCTACTGCG GAAACACGAG GGTTTGGAGA GAGATCTTGC TGCCTTGGAG 
GACAAGGTCA AAGCCCTGTG TGCCGAGGCT GACCGCCTGC AGCAGTCCCA CCCTCTGAGT 
GCCACCCAGA TCCAAGTGAA GCGAGAGGAG CTGATCACCA ACTGGGAGCA GATCCGCACC 
CTGGCCGCGG AGAGACACGC ACGTCTCAAC GATTCCTACA GGCTTCAGCG CTTCCTTGCT 
GACTTCCGAG ACCTCACCAG CTGGGTCACT GAGATGAAAG CCCTCATCAA TGCAGATGAA 
CTTGCCAATG ATGTGGCCGG GGCCGAAGCC CTGCTGGATA GGCATCAGGA GCACAAGGGT 
GAAATTGACG CTCATGAAGA TAGCTTTAAA TCTGCAGATG AGTCTGGACA GGCCCTGCTT 
GCAGCTGGTC ACTATGCCTC AGATGAAGTG AGGGAGAAGC TGACCATCCT GTCCGAGGAG 
CGGGCCGCGC TACTAGAGCT CTGGGAGCTG CGACGGCAGC AATATGAGCA GTGCATGGAC 
CTGCAGCTCT TCTACCGCGA CACGGAGCAG GTGGACAACT GGATGAGCAA ACAGGAGGCG 
TTCCTGTTGA ACGAAGACCT GGGTGATTCC TTGGATAGTG TGGAAGCGCT TCTCAAGAAG 
CACGAAGACT TTGAGAAGTC CCTCAGCGCC CAGGAGGAGA AGATCACAGC CTTAGATGAG 
TTTGCCACCA AGCTGATTCA GAACAACCAC TACGCGATGG AAGATGTGGC CACCCGGCGA 
GACGCACTGC TGAGCCGCCG CAACGCCCTC CATGAGAGAG CCATGTACCG CCGTGCCCAG 
CTTGCAGATT CCTTCCACTT GCAGCAGTTT TTCCGTGACT CTGATGAGCT CAAGAGTTGG 
GTCAACGAGA AGATGAAAAC AGCCACAGAT GAAGCTTATA AAGACCCATC CAACCTACAA 
GGGAAAGTGC AGAAGCACCA GGCCTTTGAG GCCGAGCTCT CAGCAAACCA GAGCCGCATC 
GACGCCTTGG AGAAAGCTGG GCAGAAGCTG ATCGACGTTG ACCACTATGC CAAGGATGAG 
GTGGCGACTC GGATGAATGA AGTTATCAGT CTGTGGAAGA AGCTGCTGGA GGCCACGGAG 
CTGAAAGGCA TAAAGCTCCG TGAGGCTAAC CAGCAGCAGC AGTTTAACCG CAACGTTGAG 
GACATCGAGC TGTGGCTGTA CGAAGTGGAA GGTCATCTGG CCTCGGATGA CTACGGCAAA 
GACCTCACCA ACGTCCAGAA CCTCCAGAAA AAGCATGCCC TGCTGGAGGC TGACGTGGCC 
GCTCACCAGG ATCGCATCGA TGGCATCACT ATCCAAGCCC GCCAGTTCCA GGATGCGGGC 
CACTTTGATG CTGAAAACAT CAAGAAGAAG CAGGAGGCCC TGGTAGCTCG CTACGAGGCA 
CTCAAGGAGC CCATGGTTGC CCGGAAGCAG AAGCTGGCCG ATTCCCTGCG GTTGCAGCAG 
CTCTTCCGTG ACGTTGAGGA TGAGGAGACG TGGATTCGGG AGAAAGAACC TATTGCCGCC 
TCCACCAACA GAGGCAAAGA TTTAATTGGA GTCCAGAACC TGCTAAAGAA ACACCAAGCG 
CTACAAGCAG AAATTGCTGG CCACGAGCCC CGCATCAAAG CAGTCACACA GAAGGGGAAC 
GCCATGGTAG AGGAGGGCCA TTTTGCTGCC GAGGACGTGA AGGCCAAGCT CCATGAACTG 
AACCAGAAGT GGGAGTCGCT GAAGTCCAAA GCCTCCCAGC GACGGCAGGA CCTGGAGGAC 
TCGCTGCAGG CCCAGCAGTA CTTCGCTGAT GCCAACGAGG CCGAGTCCTG GATGCGGGAG 
AAGGAGCCCA TCGTGGGCAG CACCGACTAT GGCAAAGATG AGGACTCTGC CGAGGCTCTG 
CTAAAGAAGC ACGAGGCATT GATGTCGGAT CTCAGCGCCT ATGGCAGCAG CATCCAGGCC 
CTGCGAGAGC AGGCCCAGTC GTGCCGGCAA CAAGTGGCCC CCATGGATGA CGAGACTGGG 
AAGGAGCTGG TCTTGGCGCT CTATGACTAT CAGGAGAAGA GTCCCCGAGA GGTCACCATG 
AAGAAAGGAG ACATTCTCAC CTTACTCAAC AGCACCAACA AGGACTGGTG GAAAGTGGAA 
GTGAACGACC GGCAGGGCTT CGTGCCGGCC GCCTACGTGA AGAAGCTGGA CCCAGCGCAG 
TCGGCCTCCC GGGAGAACCT CCTGGAAGAG CAGGGCAGCA TCGCGCTGCG GCAGGAGCAG 
ATTGACAACC AGACGCGCGT AACTAAGGAG GCCGGCAGCG TGTCTCTGCG TATGAAACAG 
GTGGAAGAAC TGTACCATTC TCTGCTGGAA CTGGGAGAGA AGCGTAAAGG CATGTTGGAG 
AAGAGCTGTA AGAAGTTCAT GCTGTTCCGT GAAGCGAACG AGCTGCAGCA GTGGATCAAC 
GAGAAGGAAG CGGCCCTGAC AAGCGAGGAG GTCGGCGCGG ACTTGGAGCA GGTTGAAGTG 
CTGCAGAAGA AGTTTGACGA CTTCCAGAAG GATCTCAAGG CCAATGAGTC ACGGCTGAAG 
GACATTAACA AGGTGGCTGA AGACCTGGAG TCGGAAGGCC TGATGGCGGA GGAGGTGCAG 
GCCGTGCAGC AGCAGGAGGT GTATGGCGCG ATGCCCAGGG ATGAATCTGA TTCCAAGACA 
GCCTCCCCGT GGAAGTCTGC TCGCCTGATG GTTCACACCG TGGCCACCTT TAATTCCATC 
AAGGAGCTCA ATGAGCGCTG GCGGTCCCTG CAGCAGCTGG CTGAGGAGCG GAGCCAACTC 
TTGGGCAGCG CCCACGAAGT GCAGCGGTTC CACAGAGATG CTGACGAAAC CAAAGAGTGG 
ATCGAGGAGA AGAACCAGGC TCTAAACACA GACAACTATG GCCACGACCT GGCCAGCGTC 
CAGGCCCTGC AGCGCAAGCA TGAGGGCTTC GAGAGGGACC TCGCGGCCCT TGGCGACAAG 
GTGAACTCGC TCGGGGAGAC AGCCGAGCGC CTGATCCAGT CCCACCCCGA GTCGGCAGAG 
GACCTGCAGG AGAAGTGCAC TGAGCTGAAC CAGGCATGGA GCAGCCTGGG CAAGCGTGCC 
GACCAGCGCA AGGCCAAGCT GGGCGACTCC CACGACCTGC AGCGCTTCCT CAGCGACTTC 
CGGGACCTCA TGTCTTGGAT CAATGGAATC CGAGGTCTGG TGTCCTCAGA CGAGCTAGCC 
AAGGATGTCA CTGGAGCTGA GGCCCTGCTG GAGCGACACC AGGAACACCG GACAGAAATT 
GATGCCAGAG CTGGCACTTT CCAGGCATTC GAGCAGTTTG GGCAGCAGCT GTTGGCTCAT 
GGGCACTACG CCAGCCCCGA GATCAAGGAG AAGCTCGATG TTCTAGACCA GGAGCGCGCA 
GACCTCGAGA AGGCCTGGGT CCAGCGCAGG ATGATGCTGG ATCAGTGCCT TGAATTGCAG 
CTGTTCCATC GGGATTGCGA GCAGGCCGAG AACTGGATGG CTGCCCGGGA GGCCTTCCTG 
AACACAGAAG ACAAAGGGGA CTCGCTAGAC AGCGTGGAGG CTCTGATCAA AAAACACGAG 
GACTTTGACA AAGCAATTAA TGTCCAGGAG GAGAAGATTG CTGCCCTGCA GTCCTTTGCT 
GACCAGCTCA TTGCTGGCGG TCACTACGCC AAAGGCGACA TCTCCAGCCG GCGCAATGAG 
GTTCTGGACA GGTGGCGCCG TCTAAAAGCT CAGATGATTG AGAAGAGGTC AAAGCTGGGA 
GAATCGCAGA CCCTCCAGCA GTTCAGCCGG GATGTAGACG AGATTGAAGC TTGGATCAGT 
GAAAAATTAC AGACTGCAAG TGATGAGTCA TATAAGGACC CCACCAACAT CCAGCTTTCC 
AAGCTGCTGA GCAAGCATCA GAAGCACCAG GCCTTCGAGG CAGAACTGCA CGCCAATGCG 
GACCGGATCC GCGGGGTCAT CGACGTGGGC AACTCCCTCA TTGACCGTGG GGCCTGTGCC 
GGCAGCGAGG ATGCCGTCAA GGCCCGGCTG GCCGCCTTAG CTGACCAGTG GCAGTTCCTG 
GTGCAGAAGT CGGCTGAGAA GAGCCAGAAG CTGAAAGAAG CCAACAAACA GCAGAACTTC 
AACACTGGCA TCAAGGACTT CGACTTCTGG CTCTCTGAGG TGGAAGCCCT GCTGGCCTCT 
GAGGATTATG GCAAAGACCT GGCTTCTGTG AACAACTTGC TAAAAAAACA TCAGCTGCTG 
GAGGCAGATA TCTCTGCCCA CGAGGACCGC CTGAAGGACC TCAACAGCCA GGCGGACAGC 
CTGATGACCA GCAGCGCCTT CGACACCTCC CAGGTGAAGG ATAAGCGGGA CACCATCAAC 
AGCCGTTTCC AGAAGATCAA GAGCATGGCC GCCTCCCGGC GAGCTAGGCT GAATGAGTCC 
CACCGCCTGC ACCAGTTCTT CCGGGACATG GACGATGAGG AGTCCTGGAT CAAGGAGAAG 
AAGCTGCTGG TGAGCTCAGA GGACTACGGC CGCGACCTGA CGGGCGTCCA GAACCTGAGG 
AAGAAGCACA AACGGCTGGA GGCAGAGCTG GCTGCCCACG AGCCGGCCAT TCAGGGTGTC 
CTGGACACTG GAAAGAAGCT GTCTGATGAC AACACCATCG GGAAGGAGGA GATTCAGCAG 
CGCCTGGCGC AGTTTGTGGA GCACTGGAAG GAGCTGAAGC AGCTGGCAGC TGCTCGGGGC 
CAGCGGCTAG AAGAATCTTT GGAATATCAG CAGTTTGTGG CCAACGTGGA GGAGGAGGAA 
GCCTGGATCA ATGAGAAGAT GACCCTGGTG GCCAGTGAGG ATTACGGAGA CACTCTTGCT 
GCCATCCAGG GCTTGCTGAA GAAACACGAA GCCTTCGAGA CGGACTTCAC TGTCCACAAG 
GACCGGGTGA ATGACGTCTG CACTAACGGA CAAGACCTGG TTAAGAAGAA CAATCACCAT 
GAGGAGAACA TCTCTTCCAA GATGAAGGGC TTGAACGGGA AAGTGTCAGA CTTGGAGAAG 
GCTGCCTCCC AGAGGAAGGC AAAGCTCGAC GAGAACTCGG CCTTCCTCCA GTTCAACTGG 
AAGGCCGATG TGGTGGAGTC CTGGATTGGT GAAAAGGAGA ACAGCTTGAA GACAGATGAT 
TATGGTCGAG ATCTGTCTTC TGTCCAAACT CTCCTCACCA AACAGGAAAC CTTCGATGCT 
GGCCTGCAGG CCTTCCAGCA GGAGGGCATC GCCAACATCA CTGCCCTCAA AGATCAGCTG 
CTGGCCGCCA AGCATGTTCA GTCCAAGGCC ATCGAGGCCC GCCACGCTGC CCTCATGAAG 
AGATGGAGCC AGCTCCTGGC CAATTCGGCC ACCCGGAAGA AGAAGCTGCT GGAGGCACAG 
AGTCACTTCC GCAAGGTGGA GGATCTCTTC CTGACCTTTG CCAAAAAGGC TTCAGCCTTC 
AACAGCTGGT TTGAAAATGC AGAGGAGGAC TTAACAGACC CAGTGCGCTG CAACTCCCTG 
GAAGAGATCA AGGCCTTGAG AGAGGCCCAC GATGCCTTCC GCTCCTCGCT GAGCTCAGCC 
CAGGCCGACT TCAACCAGCT GGCTGAGCTG GACCGCCAGA TCAAGAGCTT CCGCGTGGCC 
TCCAACCCCT ACACCTGGTT CACCATGGAG GCCCTGGAGG AGACCTGGAG GAACCTTCAG 
AAGATCATCA AGGAAAGGGA GCTGGAGCTG CAGAAGGAGC AGCGGCGGCA GGAGGAGAAC 
GACAAGCTGC GCCAGGAGTT CGCCCAGCAT GCCAACGCCT TCCACCAGTG GATCCAGGAG 
ACCAGAACGT ATCTCCTCGA TGGCATAGCG TATCGTCGGG TCATTCGTGT CTATCAGTAT 
GAAGTTGGGG ATGATCTGTC TGGAAGGTCC TGCATGTGGA AGAATCTGGG ACCCTTGAAT 
CGCAGCTTGA AGCTACCAAA CGCAAACACC AGGAGATTCG AGCGCATGCA GAAGTCAGCT 
CAGAAGATGA AGGACCGCTG GGGCGGCCAT GGAGGAAGCG CTGATCCTGG ACAACAAGAG 
ACACGGAGCA CGAGCACCGT GGGCCTGGCC CAGCAGTGGG ACCAGCGTCG ACCAGCTAGG 
GCATGCGGAT GCAGCACAAC CTCGGGAGCA GCAGATCCAG GCCAGAACCT GACTGTGGGA 
ACAAGCCGAC TACTGCGTCT CCACATGAAG CCTTACGTGG ACGCAAGGGC CGCGAACTCG 
CCACTGGCTT GCATTGACTA CGTTGGGAGT TCACCCGCTC GCTCTTCGGT GAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTAN1 ( XM_027966354.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_027822155.1 Ovis aries spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X1, mRNA.

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CloneID	OSm76648
Clone ID Related Accession (Same CDS sequence)	XM_027966354.1
Accession Version	XM_027966354.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7533bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-05
Organism	Ovis aries(sheep)
Product	spectrin alpha chain, non-erythrocytic 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040254.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ovis aries Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTCAT CGTTTCGTAA ACGCAGAGTC CAGAAAATGG ACCCAAGCGG GGTCAAAGTG 
CTGGAGACAG CCGAGGACAT CCAGGAGAGG CGGCAGCAGG TCCTGGACAG ATACCACCGC 
TTCAAGGAGC TCTCGACCCT GAGGCGCCAG AAGCTTGAAG ACTCCTACCG ATTCCAGTTT 
TTCCAAAGAG ATGCTGAGGA GCTGGAGAAA TGGATTCAGG AGAAGCTTCA GATTGCCTCG 
GATGAGAATT ACAAAGACCC AACCAACCTG CAGGGCAAGC TGCAGAAGCA TCAAGCCTTC 
GAAGCCGAGG TGCAGGCCAA CTCGGGTGCC ATCGTGAAGC TTGATGAGAC CGGTAACCTG 
ATGATCTCAG AAGGGCACTT CGCGTCCGAA ACCATCCGGA CTCGTCTGAT GGAGCTGCAC 
CGCCAGTGGG AATTGCTTCT GGAGAAGATG CGGGAGAAAG GCATCAAGCT GCTGCAGGCC 
CAGAAGCTGG TGCAGTACCT GCGGGAGTGT GAGGACGTGA TGGACTGGAT CAATGACAAG 
GAAGCCATTG TGACATCTGA GGAGCTGGGC CAGGACCTGG AGCACGTGGA GGTGTTACAG 
AAGAAATTCG AAGAGTTTCA GACAGACATG GCTGCCCACG AAGAGAGAGT CAATGAAGTG 
AACCAGTTTG CTGCCAAGCT CATTCAGGAG CAGCACCCTG AGGAGGAGCT GATCAAGACA 
AAGGAGGACG AGGTGAATGC AGCCTGGCAG CGGCTGAAGG GCCTGGCCCT CCAGCGGCAG 
GGGAAGCTGT TCGGGGCAGC TGAGGTCCAA CGCTTTAACA GGGATGTGGA CGAGACCATT 
AGCTGGATTA AGGAGAAGGA ACAGTTAATG GCCTCTGATG ACTTTGGCCG GGACCTGGCC 
AGTGTCCAGG CTCTACTGCG GAAACACGAG GGTTTGGAGA GAGATCTTGC TGCCTTGGAG 
GACAAGGTCA AAGCCCTGTG TGCCGAGGCT GACCGCCTGC AGCAGTCCCA CCCTCTGAGT 
GCCACCCAGA TCCAAGTGAA GCGAGAGGAG CTGATCACCA ACTGGGAGCA GATCCGCACC 
CTGGCCGCGG AGAGACACGC ACGTCTCAAC GATTCCTACA GGCTTCAGCG CTTCCTTGCT 
GACTTCCGAG ACCTCACCAG CTGGGTCACT GAGATGAAAG CCCTCATCAA TGCAGATGAA 
CTTGCCAATG ATGTGGCCGG GGCCGAAGCC CTGCTGGATA GGCATCAGGA GCACAAGGGT 
GAAATTGACG CTCATGAAGA TAGCTTTAAA TCTGCAGATG AGTCTGGACA GGCCCTGCTT 
GCAGCTGGTC ACTATGCCTC AGATGAAGTG AGGGAGAAGC TGACCATCCT GTCCGAGGAG 
CGGGCCGCGC TACTAGAGCT CTGGGAGCTG CGACGGCAGC AATATGAGCA GTGCATGGAC 
CTGCAGCTCT TCTACCGCGA CACGGAGCAG GTGGACAACT GGATGAGCAA ACAGGAGGCG 
TTCCTGTTGA ACGAAGACCT GGGTGATTCC TTGGATAGTG TGGAAGCGCT TCTCAAGAAG 
CACGAAGACT TTGAGAAGTC CCTCAGCGCC CAGGAGGAGA AGATCACAGC CTTAGATGAG 
TTTGCCACCA AGCTGATTCA GAACAACCAC TACGCGATGG AAGATGTGGC CACCCGGCGA 
GACGCACTGC TGAGCCGCCG CAACGCCCTC CATGAGAGAG CCATGTACCG CCGTGCCCAG 
CTTGCAGATT CCTTCCACTT GCAGCAGTTT TTCCGTGACT CTGATGAGCT CAAGAGTTGG 
GTCAACGAGA AGATGAAAAC AGCCACAGAT GAAGCTTATA AAGACCCATC CAACCTACAA 
GGGAAAGTGC AGAAGCACCA GGCCTTTGAG GCCGAGCTCT CAGCAAACCA GAGCCGCATC 
GACGCCTTGG AGAAAGCTGG GCAGAAGCTG ATCGACGTTG ACCACTATGC CAAGGATGAG 
GTGGCGACTC GGATGAATGA AGTTATCAGT CTGTGGAAGA AGCTGCTGGA GGCCACGGAG 
CTGAAAGGCA TAAAGCTCCG TGAGGCTAAC CAGCAGCAGC AGTTTAACCG CAACGTTGAG 
GACATCGAGC TGTGGCTGTA CGAAGTGGAA GGTCATCTGG CCTCGGATGA CTACGGCAAA 
GACCTCACCA ACGTCCAGAA CCTCCAGAAA AAGCATGCCC TGCTGGAGGC TGACGTGGCC 
GCTCACCAGG ATCGCATCGA TGGCATCACT ATCCAAGCCC GCCAGTTCCA GGATGCGGGC 
CACTTTGATG CTGAAAACAT CAAGAAGAAG CAGGAGGCCC TGGTAGCTCG CTACGAGGCA 
CTCAAGGAGC CCATGGTTGC CCGGAAGCAG AAGCTGGCCG ATTCCCTGCG GTTGCAGCAG 
CTCTTCCGTG ACGTTGAGGA TGAGGAGACG TGGATTCGGG AGAAAGAACC TATTGCCGCC 
TCCACCAACA GAGGCAAAGA TTTAATTGGA GTCCAGAACC TGCTAAAGAA ACACCAAGCG 
CTACAAGCAG AAATTGCTGG CCACGAGCCC CGCATCAAAG CAGTCACACA GAAGGGGAAC 
GCCATGGTAG AGGAGGGCCA TTTTGCTGCC GAGGACGTGA AGGCCAAGCT CCATGAACTG 
AACCAGAAGT GGGAGTCGCT GAAGTCCAAG GCCTCCCAGC GACGGCAGGA CCTGGAGGAC 
TCGCTGCAGG CCCAGCAGTA CTTCGCTGAT GCCAACGAGG CCGAGTCCTG GATGCGGGAG 
AAGGAGCCCA TCGTGGGCAG CACCGACTAT GGCAAAGATG AGGACTCTGC CGAGGCTCTG 
CTAAAGAAGC ACGAGGCATT GATGTCGGAT CTCAGCGCCT ATGGCAGCAG CATCCAGGCC 
CTGCGAGAGC AGGCCCAGTC GTGCCGGCAA CAAGTGGCCC CCATGGATGA CGAGACTGGG 
AAGGAGCTGG TCTTGGCGCT CTATGACTAT CAGGAGAAGA GTCCCCGAGA GGTCACCATG 
AAGAAAGGAG ACATTCTCAC CTTACTCAAC AGCACCAACA AGGACTGGTG GAAAGTGGAA 
GTGAACGACC GGCAGGGCTT CGTGCCGGCC GCCTACGTGA AGAAGCTGGA CCCAGCGCAG 
TCGGCCTCCC GGGAGAACCT CCTGGAAGAG CAGGGCAGCA TCGCGCTGCG GCAGGAGCAG 
ATTGACAACC AGACGCGCGT AACTAAGGAG GCCGGCAGCG TGTCTCTGCG TATGAAACAG 
GTGGAAGAAC TGTACCATTC TCTGCTGGAA CTGGGAGAGA AGCGTAAAGG CATGTTGGAG 
AAGAGCTGTA AGAAGTTCAT GCTGTTCCGT GAAGCGAACG AGCTGCAGCA GTGGATCAAC 
GAGAAGGAAG CGGCCCTGAC AAGCGAGGAG GTCGGCGCGG ACTTGGAGCA GGTCGAAGTG 
CTGCAGAAGA AGTTTGACGA CTTCCAGAAG GATCTCAAGG CCAATGAGTC ACGGCTGAAG 
GACATTAACA AGGTGGCTGA AGACCTGGAG TCGGAAGGCC TGATGGCGGA GGAGGTGCAG 
GCCGTGCAGC AGCAGGAGGT GTATGGCGCG ATGCCCAGGG ATGAATCTGA TTCCAAGACA 
GCCTCCCCGT GGAAGTCTGC TCGCCTGATG GTTCACACCG TGGCCACCTT TAATTCCATC 
AAGGAGCTCA ATGAGCGCTG GCGGTCCCTG CAGCAGCTGG CTGAGGAGCG GAGCCAACTC 
TTGGGCAGCG CCCACGAAGT GCAGCGGTTC CACAGAGATG CTGACGAAAC CAAAGAGTGG 
ATCGAGGAGA AGAACCAGGC TCTAAACACA GACAACTATG GCCACGACCT GGCCAGCGTC 
CAGGCCCTGC AGCGCAAGCA TGAGGGCTTC GAGAGGGACC TCGCGGCCCT TGGCGACAAG 
GTGAACTCGC TCGGGGAGAC AGCCGAGCGC CTGATCCAGT CCCACCCCGA GTCGGCAGAG 
GACCTGCAGG AGAAGTGCAC TGAGCTGAAC CAGGCATGGA GCAGCCTGGG CAAGCGTGCC 
GACCAGCGCA AGGCCAAGCT GGGCGACTCC CACGACCTGC AGCGCTTCCT CAGCGACTTC 
CGGGACCTCA TGTCTTGGAT CAATGGAATC CGAGGTCTGG TGTCCTCAGA CGAGCTAGCC 
AAGGATGTCA CTGGAGCTGA GGCCCTGCTG GAGCGACACC AGGAACACCG GACAGAAATT 
GATGCCAGAG CTGGCACTTT CCAGGCATTC GAGCAGTTTG GGCAGCAGCT GTTGGCTCAT 
GGGCACTACG CCAGCCCCGA GATCAAGGAG AAGCTCGATG TTCTAGACCA GGAGCGCGCA 
GACCTCGAGA AGGCCTGGGT CCAGCGCAGG ATGATGCTGG ATCAGTGCCT TGAATTGCAG 
CTGTTCCATC GGGATTGCGA GCAGGCCGAG AACTGGATGG CTGCCCGGGA GGCCTTCCTG 
AACACAGAAG ACAAAGGGGA CTCGCTAGAC AGCGTGGAGG CTCTGATCAA AAAACACGAG 
GACTTTGACA AAGCAATTAA TGTCCAGGAG GAGAAGATTG CTGCCCTGCA GTCCTTTGCT 
GACCAGCTCA TTGCTGGCGG TCACTACGCC AAAGGCGACA TCTCCAGCCG GCGCAATGAG 
GTTTTGGACA GGTGGCGCCG TCTAAAAGCT CAGATGATTG AGAAGAGGTC AAAGCTGGGA 
GAATCGCAGA CCCTCCAGCA GTTCAGCCGG GATGTAGACG AGATTGAAGC TTGGATCAGT 
GAAAAATTAC AGACTGCAAG TGATGAGTCA TATAAGGACC CCACCAACAT CCAGCTTTCC 
AAGCTGCTGA GCAAGCATCA GAAGCACCAG GCCTTCGAGG CAGAACTGCA CGCCAATGCG 
GACCGGATCC GCGGGGTCAT CGACGTGGGC AACTCCCTCA TTGACCGTGG GGCCTGTGCC 
GGCAGCGAGG ATGCCGTCAA GGCCCGGCTG GCCGCCTTAG CTGACCAGTG GCAGTTCCTG 
GTGCAGAAGT CGGCTGAGAA GAGCCAGAAG CTGAAAGAAG CCAACAAACA GCAGAACTTC 
AACACTGGCA TCAAGGACTT CGACTTCTGG CTCTCTGAGG TGGAAGCCCT GCTGGCCTCT 
GAGGATTATG GCAAAGACCT GGCTTCTGTG AACAACTTGC TAAAAAAACA TCAGCTGCTG 
GAGGCAGATA TCTCTGCCCA CGAGGACCGC CTGAAGGACC TCAACAGCCA GGCGGACAGC 
CTGATGACCA GCAGCGCCTT CGACACCTCC CAGGTGAAGG ATAAGCGGGA CACCATCAAC 
AGCCGTTTCC AGAAGATCAA GAGCATGGCC GCCTCCCGGC GAGCTAGGCT GAATGAGTCC 
CACCGCCTGC ACCAGTTCTT CCGGGACATG GACGATGAGG AGTCCTGGAT CAAGGAGAAG 
AAGCTGCTGG TGAGCTCAGA GGACTACGGC CGCGACCTGA CGGGCGTCCA GAACCTGAGG 
AAGAAGCACA AACGGCTGGA GGCAGAGCTG GCTGCCCACG AGCCGGCCAT TCAGGGTGTC 
CTGGACACTG GAAAGAAGCT GTCTGATGAC AACACCATCG GGAAGGAGGA GATTCAGCAG 
CGCCTGGCGC AGTTTGTGGA GCACTGGAAG GAGCTGAAGC AGCTGGCAGC TGCTCGGGGC 
CAGCGGCTAG AAGAATCTTT GGAATATCAG CAGTTTGTGG CCAACGTGGA GGAGGAGGAA 
GCCTGGATCA ATGAGAAGAT GACCCTGGTG GCCAGTGAGG ATTACGGAGA CACTCTTGCT 
GCCATCCAGG GCTTGCTGAA GAAACACGAA GCCTTCGAGA CGGACTTCAC TGTCCACAAG 
GACCGGGTGA ATGACGTCTG CACTAACGGA CAAGACCTGG TTAAGAAGAA CAATCACCAT 
GAGGAGAACA TCTCTTCCAA GATGAAGGGC TTGAACGGGA AAGTGTCAGA CTTGGAGAAG 
GCTGCCTCCC AGAGGAAGGC AAAGCTCGAC GAGAACTCGG CCTTCCTCCA GTTCAACTGG 
AAGGCCGATG TGGTGGAGTC CTGGATTGGT GAAAAGGAGA ACAGCTTGAA GACAGATGAT 
TATGGTCGAG ATCTGTCTTC TGTCCAAACT CTCCTCACCA AACAGGAAAC CTTCGATGCT 
GGCCTGCAGG CCTTCCAGCA GGAGGGCATC GCCAACATCA CTGCCCTCAA AGATCAGCTG 
CTGGCCGCCA AGCATGTTCA GTCCAAGGCC ATCGAGGCCC GCCACGCTGC CCTCATGAAG 
AGATGGAGCC AGCTCCTGGC CAATTCGGCC ACCCGGAAGA AGAAGCTGCT GGAGGCACAG 
AGTCACTTCC GCAAGGTGGA GGATCTCTTC CTGACCTTTG CCAAAAAGGC TTCAGCCTTC 
AACAGCTGGT TTGAAAATGC AGAGGAGGAC TTAACAGACC CAGTGCGCTG CAACTCCCTG 
GAAGAGATCA AGGCCTTGAG AGAGGCCCAC GATGCCTTCC GCTCCTCGCT GAGCTCAGCC 
CAGGCCGACT TCAACCAGCT GGCTGAGCTG GACCGCCAGA TCAAGAGCTT CCGCGTGGCC 
TCCAACCCCT ACACCTGGTT CACCATGGAG GCCCTGGAGG AGACCTGGAG GAACCTTCAG 
AAGATCATCA AGGAAAGGGA GCTGGAGCTG CAGAAGGAGC AGCGGCGGCA GGAGGAGAAC 
GACAAGCTGC GCCAGGAGTT CGCCCAGCAT GCCAACGCCT TCCACCAGTG GATCCAGGAG 
ACCAGAACGT ATCTCCTCGA TGGCATAGCG TATCGTCGGG TCATTCGTGT CTATCAGTAT 
GAAGTTGGGG ATGATCTGTC TGGAAGGTCC TGCATGGTGG AAGAATCTGG GACCCTTGAA 
TCGCAGCTTG AAGCTACCAA ACGCAAACAC CAGGAGATTC GAGCCATGAG AAGTCAGCTC 
AAGAAGATTG AAGACCTGGG GGCGGCCATG GAGGAAGCGC TGATCCTGGA CAACAAGTAC 
ACGGAGCACA GCACCGTGGG CCTGGCCCAG CAGTGGGACC AGCTCGACCA GCTGGGCATG 
CGGATGCAGC ACAACCTGGA GCAGCAGATC CAGGCCAGGA ACACGACAGG AGTGACCGAG 
GAGGCCCTCA AAGAATTCAG CATGATGTTC AAACACTTCG ACAAGGACAA GTCCGGCCGG 
CTGAATCACC AGGAGTTCAA ATCCTGTCTG CGCTCCCTGG GCTATGACCT GCCCATGGTG 
GAGGAAGGGG AGCCCGACCC TGAGTTCGAG GCCATCTTGG ATACTGTGGA TCCTAACAGG 
GACGGCCACG TCTCCCTGCA AGAGTACATG GCCTTTATGA TCAGCCGTGA GACCGAGAAT 
GTCAAGTCCA GCGAGGAGAT TGAGAGCGCC TTCCGGGCCC TGAGCTCCGA AGGGAAGCCT 
TATGTGACCA AGGAGGAGCT GTACCAGAAC CTGACTCGGG AACAAGCCGA CTACTGCGTC 
TCCCACATGA AGCCCTACGT GGACGGCAAG GGCCGCGAAC TCCCCACTGC TTTCGACTAC 
GTTGAGTTCA CCCGCTCGCT CTTCGTGAAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_027822155.1
CDS	72..7604
Translation	MLSSFRKRRVQKMDPSGVKVLETAEDIQERRQQVLDRYHRFKELSTLRRQKLEDSYRFQFFQRDAEELEKWIQEKLQIASDENYKDPTNLQGKLQKHQAFEAEVQANSGAIVKLDETGNLMISEGHFASETIRTRLMELHRQWELLLEKMREKGIKLLQAQKLVQYLRECEDVMDWINDKEAIVTSEELGQDLEHVEVLQKKFEEFQTDMAAHEERVNEVNQFAAKLIQEQHPEEELIKTKEDEVNAAWQRLKGLALQRQGKLFGAAEVQRFNRDVDETISWIKEKEQLMASDDFGRDLASVQALLRKHEGLERDLAALEDKVKALCAEADRLQQSHPLSATQIQVKREELITNWEQIRTLAAERHARLNDSYRLQRFLADFRDLTSWVTEMKALINADELANDVAGAEALLDRHQEHKGEIDAHEDSFKSADESGQALLAAGHYASDEVREKLTILSEERAALLELWELRRQQYEQCMDLQLFYRDTEQVDNWMSKQEAFLLNEDLGDSLDSVEALLKKHEDFEKSLSAQEEKITALDEFATKLIQNNHYAMEDVATRRDALLSRRNALHERAMYRRAQLADSFHLQQFFRDSDELKSWVNEKMKTATDEAYKDPSNLQGKVQKHQAFEAELSANQSRIDALEKAGQKLIDVDHYAKDEVATRMNEVISLWKKLLEATELKGIKLREANQQQQFNRNVEDIELWLYEVEGHLASDDYGKDLTNVQNLQKKHALLEADVAAHQDRIDGITIQARQFQDAGHFDAENIKKKQEALVARYEALKEPMVARKQKLADSLRLQQLFRDVEDEETWIREKEPIAASTNRGKDLIGVQNLLKKHQALQAEIAGHEPRIKAVTQKGNAMVEEGHFAAEDVKAKLHELNQKWESLKSKASQRRQDLEDSLQAQQYFADANEAESWMREKEPIVGSTDYGKDEDSAEALLKKHEALMSDLSAYGSSIQALREQAQSCRQQVAPMDDETGKELVLALYDYQEKSPREVTMKKGDILTLLNSTNKDWWKVEVNDRQGFVPAAYVKKLDPAQSASRENLLEEQGSIALRQEQIDNQTRVTKEAGSVSLRMKQVEELYHSLLELGEKRKGMLEKSCKKFMLFREANELQQWINEKEAALTSEEVGADLEQVEVLQKKFDDFQKDLKANESRLKDINKVAEDLESEGLMAEEVQAVQQQEVYGAMPRDESDSKTASPWKSARLMVHTVATFNSIKELNERWRSLQQLAEERSQLLGSAHEVQRFHRDADETKEWIEEKNQALNTDNYGHDLASVQALQRKHEGFERDLAALGDKVNSLGETAERLIQSHPESAEDLQEKCTELNQAWSSLGKRADQRKAKLGDSHDLQRFLSDFRDLMSWINGIRGLVSSDELAKDVTGAEALLERHQEHRTEIDARAGTFQAFEQFGQQLLAHGHYASPEIKEKLDVLDQERADLEKAWVQRRMMLDQCLELQLFHRDCEQAENWMAAREAFLNTEDKGDSLDSVEALIKKHEDFDKAINVQEEKIAALQSFADQLIAGGHYAKGDISSRRNEVLDRWRRLKAQMIEKRSKLGESQTLQQFSRDVDEIEAWISEKLQTASDESYKDPTNIQLSKLLSKHQKHQAFEAELHANADRIRGVIDVGNSLIDRGACAGSEDAVKARLAALADQWQFLVQKSAEKSQKLKEANKQQNFNTGIKDFDFWLSEVEALLASEDYGKDLASVNNLLKKHQLLEADISAHEDRLKDLNSQADSLMTSSAFDTSQVKDKRDTINSRFQKIKSMAASRRARLNESHRLHQFFRDMDDEESWIKEKKLLVSSEDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQFVEHWKELKQLAAARGQRLEESLEYQQFVANVEEEEAWINEKMTLVASEDYGDTLAAIQGLLKKHEAFETDFTVHKDRVNDVCTNGQDLVKKNNHHEENISSKMKGLNGKVSDLEKAASQRKAKLDENSAFLQFNWKADVVESWIGEKENSLKTDDYGRDLSSVQTLLTKQETFDAGLQAFQQEGIANITALKDQLLAAKHVQSKAIEARHAALMKRWSQLLANSATRKKKLLEAQSHFRKVEDLFLTFAKKASAFNSWFENAEEDLTDPVRCNSLEEIKALREAHDAFRSSLSSAQADFNQLAELDRQIKSFRVASNPYTWFTMEALEETWRNLQKIIKERELELQKEQRRQEENDKLRQEFAQHANAFHQWIQETRTYLLDGIAYRRVIRVYQYEVGDDLSGRSCMVEESGTLESQLEATKRKHQEIRAMRSQLKKIEDLGAAMEEALILDNKYTEHSTVGLAQQWDQLDQLGMRMQHNLEQQIQARNTTGVTEEALKEFSMMFKHFDKDKSGRLNHQEFKSCLRSLGYDLPMVEEGEPDPEFEAILDTVDPNRDGHVSLQEYMAFMISRETENVKSSEEIESAFRALSSEGKPYVTKEELYQNLTREQADYCVSHMKPYVDGKGRELPTAFDYVEFTRSLFVN

Target ORF information:

RefSeq Version	XM_027966354.1
Organism	Ovis aries(sheep)
Definition	Ovis aries spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027966354.1

ORF Insert Sequence:

ATGCTGTCAT CGTTTCGTAA ACGCAGAGTC CAGAAAATGG ACCCAAGCGG GGTCAAAGTG 
CTGGAGACAG CCGAGGACAT CCAGGAGAGG CGGCAGCAGG TCCTGGACAG ATACCACCGC 
TTCAAGGAGC TCTCGACCCT GAGGCGCCAG AAGCTTGAAG ACTCCTACCG ATTCCAGTTT 
TTCCAAAGAG ATGCTGAGGA GCTGGAGAAA TGGATTCAGG AGAAGCTTCA GATTGCCTCG 
GATGAGAATT ACAAAGACCC AACCAACCTG CAGGGCAAGC TGCAGAAGCA TCAAGCCTTC 
GAAGCCGAGG TGCAGGCCAA CTCGGGTGCC ATCGTGAAGC TTGATGAGAC CGGTAACCTG 
ATGATCTCAG AAGGGCACTT CGCGTCCGAA ACCATCCGGA CTCGTCTGAT GGAGCTGCAC 
CGCCAGTGGG AATTGCTTCT GGAGAAGATG CGGGAGAAAG GCATCAAGCT GCTGCAGGCC 
CAGAAGCTGG TGCAGTACCT GCGGGAGTGT GAGGACGTGA TGGACTGGAT CAATGACAAG 
GAAGCCATTG TGACATCTGA GGAGCTGGGC CAGGACCTGG AGCACGTGGA GGTGTTACAG 
AAGAAATTCG AAGAGTTTCA GACAGACATG GCTGCCCACG AAGAGAGAGT CAATGAAGTG 
AACCAGTTTG CTGCCAAGCT CATTCAGGAG CAGCACCCTG AGGAGGAGCT GATCAAGACA 
AAGGAGGACG AGGTGAATGC AGCCTGGCAG CGGCTGAAGG GCCTGGCCCT CCAGCGGCAG 
GGGAAGCTGT TCGGGGCAGC TGAGGTCCAA CGCTTTAACA GGGATGTGGA CGAGACCATT 
AGCTGGATTA AGGAGAAGGA ACAGTTAATG GCCTCTGATG ACTTTGGCCG GGACCTGGCC 
AGTGTCCAGG CTCTACTGCG GAAACACGAG GGTTTGGAGA GAGATCTTGC TGCCTTGGAG 
GACAAGGTCA AAGCCCTGTG TGCCGAGGCT GACCGCCTGC AGCAGTCCCA CCCTCTGAGT 
GCCACCCAGA TCCAAGTGAA GCGAGAGGAG CTGATCACCA ACTGGGAGCA GATCCGCACC 
CTGGCCGCGG AGAGACACGC ACGTCTCAAC GATTCCTACA GGCTTCAGCG CTTCCTTGCT 
GACTTCCGAG ACCTCACCAG CTGGGTCACT GAGATGAAAG CCCTCATCAA TGCAGATGAA 
CTTGCCAATG ATGTGGCCGG GGCCGAAGCC CTGCTGGATA GGCATCAGGA GCACAAGGGT 
GAAATTGACG CTCATGAAGA TAGCTTTAAA TCTGCAGATG AGTCTGGACA GGCCCTGCTT 
GCAGCTGGTC ACTATGCCTC AGATGAAGTG AGGGAGAAGC TGACCATCCT GTCCGAGGAG 
CGGGCCGCGC TACTAGAGCT CTGGGAGCTG CGACGGCAGC AATATGAGCA GTGCATGGAC 
CTGCAGCTCT TCTACCGCGA CACGGAGCAG GTGGACAACT GGATGAGCAA ACAGGAGGCG 
TTCCTGTTGA ACGAAGACCT GGGTGATTCC TTGGATAGTG TGGAAGCGCT TCTCAAGAAG 
CACGAAGACT TTGAGAAGTC CCTCAGCGCC CAGGAGGAGA AGATCACAGC CTTAGATGAG 
TTTGCCACCA AGCTGATTCA GAACAACCAC TACGCGATGG AAGATGTGGC CACCCGGCGA 
GACGCACTGC TGAGCCGCCG CAACGCCCTC CATGAGAGAG CCATGTACCG CCGTGCCCAG 
CTTGCAGATT CCTTCCACTT GCAGCAGTTT TTCCGTGACT CTGATGAGCT CAAGAGTTGG 
GTCAACGAGA AGATGAAAAC AGCCACAGAT GAAGCTTATA AAGACCCATC CAACCTACAA 
GGGAAAGTGC AGAAGCACCA GGCCTTTGAG GCCGAGCTCT CAGCAAACCA GAGCCGCATC 
GACGCCTTGG AGAAAGCTGG GCAGAAGCTG ATCGACGTTG ACCACTATGC CAAGGATGAG 
GTGGCGACTC GGATGAATGA AGTTATCAGT CTGTGGAAGA AGCTGCTGGA GGCCACGGAG 
CTGAAAGGCA TAAAGCTCCG TGAGGCTAAC CAGCAGCAGC AGTTTAACCG CAACGTTGAG 
GACATCGAGC TGTGGCTGTA CGAAGTGGAA GGTCATCTGG CCTCGGATGA CTACGGCAAA 
GACCTCACCA ACGTCCAGAA CCTCCAGAAA AAGCATGCCC TGCTGGAGGC TGACGTGGCC 
GCTCACCAGG ATCGCATCGA TGGCATCACT ATCCAAGCCC GCCAGTTCCA GGATGCGGGC 
CACTTTGATG CTGAAAACAT CAAGAAGAAG CAGGAGGCCC TGGTAGCTCG CTACGAGGCA 
CTCAAGGAGC CCATGGTTGC CCGGAAGCAG AAGCTGGCCG ATTCCCTGCG GTTGCAGCAG 
CTCTTCCGTG ACGTTGAGGA TGAGGAGACG TGGATTCGGG AGAAAGAACC TATTGCCGCC 
TCCACCAACA GAGGCAAAGA TTTAATTGGA GTCCAGAACC TGCTAAAGAA ACACCAAGCG 
CTACAAGCAG AAATTGCTGG CCACGAGCCC CGCATCAAAG CAGTCACACA GAAGGGGAAC 
GCCATGGTAG AGGAGGGCCA TTTTGCTGCC GAGGACGTGA AGGCCAAGCT CCATGAACTG 
AACCAGAAGT GGGAGTCGCT GAAGTCCAAG GCCTCCCAGC GACGGCAGGA CCTGGAGGAC 
TCGCTGCAGG CCCAGCAGTA CTTCGCTGAT GCCAACGAGG CCGAGTCCTG GATGCGGGAG 
AAGGAGCCCA TCGTGGGCAG CACCGACTAT GGCAAAGATG AGGACTCTGC CGAGGCTCTG 
CTAAAGAAGC ACGAGGCATT GATGTCGGAT CTCAGCGCCT ATGGCAGCAG CATCCAGGCC 
CTGCGAGAGC AGGCCCAGTC GTGCCGGCAA CAAGTGGCCC CCATGGATGA CGAGACTGGG 
AAGGAGCTGG TCTTGGCGCT CTATGACTAT CAGGAGAAGA GTCCCCGAGA GGTCACCATG 
AAGAAAGGAG ACATTCTCAC CTTACTCAAC AGCACCAACA AGGACTGGTG GAAAGTGGAA 
GTGAACGACC GGCAGGGCTT CGTGCCGGCC GCCTACGTGA AGAAGCTGGA CCCAGCGCAG 
TCGGCCTCCC GGGAGAACCT CCTGGAAGAG CAGGGCAGCA TCGCGCTGCG GCAGGAGCAG 
ATTGACAACC AGACGCGCGT AACTAAGGAG GCCGGCAGCG TGTCTCTGCG TATGAAACAG 
GTGGAAGAAC TGTACCATTC TCTGCTGGAA CTGGGAGAGA AGCGTAAAGG CATGTTGGAG 
AAGAGCTGTA AGAAGTTCAT GCTGTTCCGT GAAGCGAACG AGCTGCAGCA GTGGATCAAC 
GAGAAGGAAG CGGCCCTGAC AAGCGAGGAG GTCGGCGCGG ACTTGGAGCA GGTCGAAGTG 
CTGCAGAAGA AGTTTGACGA CTTCCAGAAG GATCTCAAGG CCAATGAGTC ACGGCTGAAG 
GACATTAACA AGGTGGCTGA AGACCTGGAG TCGGAAGGCC TGATGGCGGA GGAGGTGCAG 
GCCGTGCAGC AGCAGGAGGT GTATGGCGCG ATGCCCAGGG ATGAATCTGA TTCCAAGACA 
GCCTCCCCGT GGAAGTCTGC TCGCCTGATG GTTCACACCG TGGCCACCTT TAATTCCATC 
AAGGAGCTCA ATGAGCGCTG GCGGTCCCTG CAGCAGCTGG CTGAGGAGCG GAGCCAACTC 
TTGGGCAGCG CCCACGAAGT GCAGCGGTTC CACAGAGATG CTGACGAAAC CAAAGAGTGG 
ATCGAGGAGA AGAACCAGGC TCTAAACACA GACAACTATG GCCACGACCT GGCCAGCGTC 
CAGGCCCTGC AGCGCAAGCA TGAGGGCTTC GAGAGGGACC TCGCGGCCCT TGGCGACAAG 
GTGAACTCGC TCGGGGAGAC AGCCGAGCGC CTGATCCAGT CCCACCCCGA GTCGGCAGAG 
GACCTGCAGG AGAAGTGCAC TGAGCTGAAC CAGGCATGGA GCAGCCTGGG CAAGCGTGCC 
GACCAGCGCA AGGCCAAGCT GGGCGACTCC CACGACCTGC AGCGCTTCCT CAGCGACTTC 
CGGGACCTCA TGTCTTGGAT CAATGGAATC CGAGGTCTGG TGTCCTCAGA CGAGCTAGCC 
AAGGATGTCA CTGGAGCTGA GGCCCTGCTG GAGCGACACC AGGAACACCG GACAGAAATT 
GATGCCAGAG CTGGCACTTT CCAGGCATTC GAGCAGTTTG GGCAGCAGCT GTTGGCTCAT 
GGGCACTACG CCAGCCCCGA GATCAAGGAG AAGCTCGATG TTCTAGACCA GGAGCGCGCA 
GACCTCGAGA AGGCCTGGGT CCAGCGCAGG ATGATGCTGG ATCAGTGCCT TGAATTGCAG 
CTGTTCCATC GGGATTGCGA GCAGGCCGAG AACTGGATGG CTGCCCGGGA GGCCTTCCTG 
AACACAGAAG ACAAAGGGGA CTCGCTAGAC AGCGTGGAGG CTCTGATCAA AAAACACGAG 
GACTTTGACA AAGCAATTAA TGTCCAGGAG GAGAAGATTG CTGCCCTGCA GTCCTTTGCT 
GACCAGCTCA TTGCTGGCGG TCACTACGCC AAAGGCGACA TCTCCAGCCG GCGCAATGAG 
GTTTTGGACA GGTGGCGCCG TCTAAAAGCT CAGATGATTG AGAAGAGGTC AAAGCTGGGA 
GAATCGCAGA CCCTCCAGCA GTTCAGCCGG GATGTAGACG AGATTGAAGC TTGGATCAGT 
GAAAAATTAC AGACTGCAAG TGATGAGTCA TATAAGGACC CCACCAACAT CCAGCTTTCC 
AAGCTGCTGA GCAAGCATCA GAAGCACCAG GCCTTCGAGG CAGAACTGCA CGCCAATGCG 
GACCGGATCC GCGGGGTCAT CGACGTGGGC AACTCCCTCA TTGACCGTGG GGCCTGTGCC 
GGCAGCGAGG ATGCCGTCAA GGCCCGGCTG GCCGCCTTAG CTGACCAGTG GCAGTTCCTG 
GTGCAGAAGT CGGCTGAGAA GAGCCAGAAG CTGAAAGAAG CCAACAAACA GCAGAACTTC 
AACACTGGCA TCAAGGACTT CGACTTCTGG CTCTCTGAGG TGGAAGCCCT GCTGGCCTCT 
GAGGATTATG GCAAAGACCT GGCTTCTGTG AACAACTTGC TAAAAAAACA TCAGCTGCTG 
GAGGCAGATA TCTCTGCCCA CGAGGACCGC CTGAAGGACC TCAACAGCCA GGCGGACAGC 
CTGATGACCA GCAGCGCCTT CGACACCTCC CAGGTGAAGG ATAAGCGGGA CACCATCAAC 
AGCCGTTTCC AGAAGATCAA GAGCATGGCC GCCTCCCGGC GAGCTAGGCT GAATGAGTCC 
CACCGCCTGC ACCAGTTCTT CCGGGACATG GACGATGAGG AGTCCTGGAT CAAGGAGAAG 
AAGCTGCTGG TGAGCTCAGA GGACTACGGC CGCGACCTGA CGGGCGTCCA GAACCTGAGG 
AAGAAGCACA AACGGCTGGA GGCAGAGCTG GCTGCCCACG AGCCGGCCAT TCAGGGTGTC 
CTGGACACTG GAAAGAAGCT GTCTGATGAC AACACCATCG GGAAGGAGGA GATTCAGCAG 
CGCCTGGCGC AGTTTGTGGA GCACTGGAAG GAGCTGAAGC AGCTGGCAGC TGCTCGGGGC 
CAGCGGCTAG AAGAATCTTT GGAATATCAG CAGTTTGTGG CCAACGTGGA GGAGGAGGAA 
GCCTGGATCA ATGAGAAGAT GACCCTGGTG GCCAGTGAGG ATTACGGAGA CACTCTTGCT 
GCCATCCAGG GCTTGCTGAA GAAACACGAA GCCTTCGAGA CGGACTTCAC TGTCCACAAG 
GACCGGGTGA ATGACGTCTG CACTAACGGA CAAGACCTGG TTAAGAAGAA CAATCACCAT 
GAGGAGAACA TCTCTTCCAA GATGAAGGGC TTGAACGGGA AAGTGTCAGA CTTGGAGAAG 
GCTGCCTCCC AGAGGAAGGC AAAGCTCGAC GAGAACTCGG CCTTCCTCCA GTTCAACTGG 
AAGGCCGATG TGGTGGAGTC CTGGATTGGT GAAAAGGAGA ACAGCTTGAA GACAGATGAT 
TATGGTCGAG ATCTGTCTTC TGTCCAAACT CTCCTCACCA AACAGGAAAC CTTCGATGCT 
GGCCTGCAGG CCTTCCAGCA GGAGGGCATC GCCAACATCA CTGCCCTCAA AGATCAGCTG 
CTGGCCGCCA AGCATGTTCA GTCCAAGGCC ATCGAGGCCC GCCACGCTGC CCTCATGAAG 
AGATGGAGCC AGCTCCTGGC CAATTCGGCC ACCCGGAAGA AGAAGCTGCT GGAGGCACAG 
AGTCACTTCC GCAAGGTGGA GGATCTCTTC CTGACCTTTG CCAAAAAGGC TTCAGCCTTC 
AACAGCTGGT TTGAAAATGC AGAGGAGGAC TTAACAGACC CAGTGCGCTG CAACTCCCTG 
GAAGAGATCA AGGCCTTGAG AGAGGCCCAC GATGCCTTCC GCTCCTCGCT GAGCTCAGCC 
CAGGCCGACT TCAACCAGCT GGCTGAGCTG GACCGCCAGA TCAAGAGCTT CCGCGTGGCC 
TCCAACCCCT ACACCTGGTT CACCATGGAG GCCCTGGAGG AGACCTGGAG GAACCTTCAG 
AAGATCATCA AGGAAAGGGA GCTGGAGCTG CAGAAGGAGC AGCGGCGGCA GGAGGAGAAC 
GACAAGCTGC GCCAGGAGTT CGCCCAGCAT GCCAACGCCT TCCACCAGTG GATCCAGGAG 
ACCAGAACGT ATCTCCTCGA TGGCATAGCG TATCGTCGGG TCATTCGTGT CTATCAGTAT 
GAAGTTGGGG ATGATCTGTC TGGAAGGTCC TGCATGGTGG AAGAATCTGG GACCCTTGAA 
TCGCAGCTTG AAGCTACCAA ACGCAAACAC CAGGAGATTC GAGCCATGAG AAGTCAGCTC 
AAGAAGATTG AAGACCTGGG GGCGGCCATG GAGGAAGCGC TGATCCTGGA CAACAAGTAC 
ACGGAGCACA GCACCGTGGG CCTGGCCCAG CAGTGGGACC AGCTCGACCA GCTGGGCATG 
CGGATGCAGC ACAACCTGGA GCAGCAGATC CAGGCCAGGA ACACGACAGG AGTGACCGAG 
GAGGCCCTCA AAGAATTCAG CATGATGTTC AAACACTTCG ACAAGGACAA GTCCGGCCGG 
CTGAATCACC AGGAGTTCAA ATCCTGTCTG CGCTCCCTGG GCTATGACCT GCCCATGGTG 
GAGGAAGGGG AGCCCGACCC TGAGTTCGAG GCCATCTTGG ATACTGTGGA TCCTAACAGG 
GACGGCCACG TCTCCCTGCA AGAGTACATG GCCTTTATGA TCAGCCGTGA GACCGAGAAT 
GTCAAGTCCA GCGAGGAGAT TGAGAGCGCC TTCCGGGCCC TGAGCTCCGA AGGGAAGCCT 
TATGTGACCA AGGAGGAGCT GTACCAGAAC CTGACTCGGG AACAAGCCGA CTACTGCGTC 
TCCCACATGA AGCCCTACGT GGACGGCAAG GGCCGCGAAC TCCCCACTGC TTTCGACTAC 
GTTGAGTTCA CCCGCTCGCT CTTCGTGAAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTAN1 ( XM_027966355.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_027822156.1 Ovis aries spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X2, mRNA.

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CloneID	OSm76649
Clone ID Related Accession (Same CDS sequence)	XM_027966355.1
Accession Version	XM_027966355.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7518bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-05
Organism	Ovis aries(sheep)
Product	spectrin alpha chain, non-erythrocytic 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040254.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ovis aries Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTCAT CGTTTCGTAA ACGCAGAGTC CAGAAAATGG ACCCAAGCGG GGTCAAAGTG 
CTGGAGACAG CCGAGGACAT CCAGGAGAGG CGGCAGCAGG TCCTGGACAG ATACCACCGC 
TTCAAGGAGC TCTCGACCCT GAGGCGCCAG AAGCTTGAAG ACTCCTACCG ATTCCAGTTT 
TTCCAAAGAG ATGCTGAGGA GCTGGAGAAA TGGATTCAGG AGAAGCTTCA GATTGCCTCG 
GATGAGAATT ACAAAGACCC AACCAACCTG CAGGGCAAGC TGCAGAAGCA TCAAGCCTTC 
GAAGCCGAGG TGCAGGCCAA CTCGGGTGCC ATCGTGAAGC TTGATGAGAC CGGTAACCTG 
ATGATCTCAG AAGGGCACTT CGCGTCCGAA ACCATCCGGA CTCGTCTGAT GGAGCTGCAC 
CGCCAGTGGG AATTGCTTCT GGAGAAGATG CGGGAGAAAG GCATCAAGCT GCTGCAGGCC 
CAGAAGCTGG TGCAGTACCT GCGGGAGTGT GAGGACGTGA TGGACTGGAT CAATGACAAG 
GAAGCCATTG TGACATCTGA GGAGCTGGGC CAGGACCTGG AGCACGTGGA GGTGTTACAG 
AAGAAATTCG AAGAGTTTCA GACAGACATG GCTGCCCACG AAGAGAGAGT CAATGAAGTG 
AACCAGTTTG CTGCCAAGCT CATTCAGGAG CAGCACCCTG AGGAGGAGCT GATCAAGACA 
AAGGAGGACG AGGTGAATGC AGCCTGGCAG CGGCTGAAGG GCCTGGCCCT CCAGCGGCAG 
GGGAAGCTGT TCGGGGCAGC TGAGGTCCAA CGCTTTAACA GGGATGTGGA CGAGACCATT 
AGCTGGATTA AGGAGAAGGA ACAGTTAATG GCCTCTGATG ACTTTGGCCG GGACCTGGCC 
AGTGTCCAGG CTCTACTGCG GAAACACGAG GGTTTGGAGA GAGATCTTGC TGCCTTGGAG 
GACAAGGTCA AAGCCCTGTG TGCCGAGGCT GACCGCCTGC AGCAGTCCCA CCCTCTGAGT 
GCCACCCAGA TCCAAGTGAA GCGAGAGGAG CTGATCACCA ACTGGGAGCA GATCCGCACC 
CTGGCCGCGG AGAGACACGC ACGTCTCAAC GATTCCTACA GGCTTCAGCG CTTCCTTGCT 
GACTTCCGAG ACCTCACCAG CTGGGTCACT GAGATGAAAG CCCTCATCAA TGCAGATGAA 
CTTGCCAATG ATGTGGCCGG GGCCGAAGCC CTGCTGGATA GGCATCAGGA GCACAAGGGT 
GAAATTGACG CTCATGAAGA TAGCTTTAAA TCTGCAGATG AGTCTGGACA GGCCCTGCTT 
GCAGCTGGTC ACTATGCCTC AGATGAAGTG AGGGAGAAGC TGACCATCCT GTCCGAGGAG 
CGGGCCGCGC TACTAGAGCT CTGGGAGCTG CGACGGCAGC AATATGAGCA GTGCATGGAC 
CTGCAGCTCT TCTACCGCGA CACGGAGCAG GTGGACAACT GGATGAGCAA ACAGGAGGCG 
TTCCTGTTGA ACGAAGACCT GGGTGATTCC TTGGATAGTG TGGAAGCGCT TCTCAAGAAG 
CACGAAGACT TTGAGAAGTC CCTCAGCGCC CAGGAGGAGA AGATCACAGC CTTAGATGAG 
TTTGCCACCA AGCTGATTCA GAACAACCAC TACGCGATGG AAGATGTGGC CACCCGGCGA 
GACGCACTGC TGAGCCGCCG CAACGCCCTC CATGAGAGAG CCATGTACCG CCGTGCCCAG 
CTTGCAGATT CCTTCCACTT GCAGCAGTTT TTCCGTGACT CTGATGAGCT CAAGAGTTGG 
GTCAACGAGA AGATGAAAAC AGCCACAGAT GAAGCTTATA AAGACCCATC CAACCTACAA 
GGGAAAGTGC AGAAGCACCA GGCCTTTGAG GCCGAGCTCT CAGCAAACCA GAGCCGCATC 
GACGCCTTGG AGAAAGCTGG GCAGAAGCTG ATCGACGTTG ACCACTATGC CAAGGATGAG 
GTGGCGACTC GGATGAATGA AGTTATCAGT CTGTGGAAGA AGCTGCTGGA GGCCACGGAG 
CTGAAAGGCA TAAAGCTCCG TGAGGCTAAC CAGCAGCAGC AGTTTAACCG CAACGTTGAG 
GACATCGAGC TGTGGCTGTA CGAAGTGGAA GGTCATCTGG CCTCGGATGA CTACGGCAAA 
GACCTCACCA ACGTCCAGAA CCTCCAGAAA AAGCATGCCC TGCTGGAGGC TGACGTGGCC 
GCTCACCAGG ATCGCATCGA TGGCATCACT ATCCAAGCCC GCCAGTTCCA GGATGCGGGC 
CACTTTGATG CTGAAAACAT CAAGAAGAAG CAGGAGGCCC TGGTAGCTCG CTACGAGGCA 
CTCAAGGAGC CCATGGTTGC CCGGAAGCAG AAGCTGGCCG ATTCCCTGCG GTTGCAGCAG 
CTCTTCCGTG ACGTTGAGGA TGAGGAGACG TGGATTCGGG AGAAAGAACC TATTGCCGCC 
TCCACCAACA GAGGCAAAGA TTTAATTGGA GTCCAGAACC TGCTAAAGAA ACACCAAGCG 
CTACAAGCAG AAATTGCTGG CCACGAGCCC CGCATCAAAG CAGTCACACA GAAGGGGAAC 
GCCATGGTAG AGGAGGGCCA TTTTGCTGCC GAGGACGTGA AGGCCAAGCT CCATGAACTG 
AACCAGAAGT GGGAGTCGCT GAAGTCCAAG GCCTCCCAGC GACGGCAGGA CCTGGAGGAC 
TCGCTGCAGG CCCAGCAGTA CTTCGCTGAT GCCAACGAGG CCGAGTCCTG GATGCGGGAG 
AAGGAGCCCA TCGTGGGCAG CACCGACTAT GGCAAAGATG AGGACTCTGC CGAGGCTCTG 
CTAAAGAAGC ACGAGGCATT GATGTCGGAT CTCAGCGCCT ATGGCAGCAG CATCCAGGCC 
CTGCGAGAGC AGGCCCAGTC GTGCCGGCAA CAAGTGGCCC CCATGGATGA CGAGACTGGG 
AAGGAGCTGG TCTTGGCGCT CTATGACTAT CAGGAGAAGA GTCCCCGAGA GGTCACCATG 
AAGAAAGGAG ACATTCTCAC CTTACTCAAC AGCACCAACA AGGACTGGTG GAAAGTGGAA 
GTGAACGACC GGCAGGGCTT CGTGCCGGCC GCCTACGTGA AGAAGCTGGA CCCAGCGCAG 
TCGGCCTCCC GGGAGAACCT CCTGGAAGAG CAGGGCAGCA TCGCGCTGCG GCAGGAGCAG 
ATTGACAACC AGACGCGCGT AACTAAGGAG GCCGGCAGCG TGTCTCTGCG TATGAAACAG 
GTGGAAGAAC TGTACCATTC TCTGCTGGAA CTGGGAGAGA AGCGTAAAGG CATGTTGGAG 
AAGAGCTGTA AGAAGTTCAT GCTGTTCCGT GAAGCGAACG AGCTGCAGCA GTGGATCAAC 
GAGAAGGAAG CGGCCCTGAC AAGCGAGGAG GTCGGCGCGG ACTTGGAGCA GGTCGAAGTG 
CTGCAGAAGA AGTTTGACGA CTTCCAGAAG GATCTCAAGG CCAATGAGTC ACGGCTGAAG 
GACATTAACA AGGTGGCTGA AGACCTGGAG TCGGAAGGCC TGATGGCGGA GGAGGTGCAG 
GCCGTGCAGC AGCAGGAGGT GTATGGCGCG ATGCCCAGGG ATGAATCTGA TTCCAAGACA 
GCCTCCCCGT GGAAGTCTGC TCGCCTGATG GTTCACACCG TGGCCACCTT TAATTCCATC 
AAGGAGCTCA ATGAGCGCTG GCGGTCCCTG CAGCAGCTGG CTGAGGAGCG GAGCCAACTC 
TTGGGCAGCG CCCACGAAGT GCAGCGGTTC CACAGAGATG CTGACGAAAC CAAAGAGTGG 
ATCGAGGAGA AGAACCAGGC TCTAAACACA GACAACTATG GCCACGACCT GGCCAGCGTC 
CAGGCCCTGC AGCGCAAGCA TGAGGGCTTC GAGAGGGACC TCGCGGCCCT TGGCGACAAG 
GTGAACTCGC TCGGGGAGAC AGCCGAGCGC CTGATCCAGT CCCACCCCGA GTCGGCAGAG 
GACCTGCAGG AGAAGTGCAC TGAGCTGAAC CAGGCATGGA GCAGCCTGGG CAAGCGTGCC 
GACCAGCGCA AGGCCAAGCT GGGCGACTCC CACGACCTGC AGCGCTTCCT CAGCGACTTC 
CGGGACCTCA TGTCTTGGAT CAATGGAATC CGAGGTCTGG TGTCCTCAGA CGAGCTAGCC 
AAGGATGTCA CTGGAGCTGA GGCCCTGCTG GAGCGACACC AGGAACACCG GACAGAAATT 
GATGCCAGAG CTGGCACTTT CCAGGCATTC GAGCAGTTTG GGCAGCAGCT GTTGGCTCAT 
GGGCACTACG CCAGCCCCGA GATCAAGGAG AAGCTCGATG TTCTAGACCA GGAGCGCGCA 
GACCTCGAGA AGGCCTGGGT CCAGCGCAGG ATGATGCTGG ATCAGTGCCT TGAATTGCAG 
CTGTTCCATC GGGATTGCGA GCAGGCCGAG AACTGGATGG CTGCCCGGGA GGCCTTCCTG 
AACACAGAAG ACAAAGGGGA CTCGCTAGAC AGCGTGGAGG CTCTGATCAA AAAACACGAG 
GACTTTGACA AAGCAATTAA TGTCCAGGAG GAGAAGATTG CTGCCCTGCA GTCCTTTGCT 
GACCAGCTCA TTGCTGGCGG TCACTACGCC AAAGGCGACA TCTCCAGCCG GCGCAATGAG 
GTTTTGGACA GGTGGCGCCG TCTAAAAGCT CAGATGATTG AGAAGAGGTC AAAGCTGGGA 
GAATCGCAGA CCCTCCAGCA GTTCAGCCGG GATGTAGACG AGATTGAAGC TTGGATCAGT 
GAAAAATTAC AGACTGCAAG TGATGAGTCA TATAAGGACC CCACCAACAT CCAGAGCAAG 
CATCAGAAGC ACCAGGCCTT CGAGGCAGAA CTGCACGCCA ATGCGGACCG GATCCGCGGG 
GTCATCGACG TGGGCAACTC CCTCATTGAC CGTGGGGCCT GTGCCGGCAG CGAGGATGCC 
GTCAAGGCCC GGCTGGCCGC CTTAGCTGAC CAGTGGCAGT TCCTGGTGCA GAAGTCGGCT 
GAGAAGAGCC AGAAGCTGAA AGAAGCCAAC AAACAGCAGA ACTTCAACAC TGGCATCAAG 
GACTTCGACT TCTGGCTCTC TGAGGTGGAA GCCCTGCTGG CCTCTGAGGA TTATGGCAAA 
GACCTGGCTT CTGTGAACAA CTTGCTAAAA AAACATCAGC TGCTGGAGGC AGATATCTCT 
GCCCACGAGG ACCGCCTGAA GGACCTCAAC AGCCAGGCGG ACAGCCTGAT GACCAGCAGC 
GCCTTCGACA CCTCCCAGGT GAAGGATAAG CGGGACACCA TCAACAGCCG TTTCCAGAAG 
ATCAAGAGCA TGGCCGCCTC CCGGCGAGCT AGGCTGAATG AGTCCCACCG CCTGCACCAG 
TTCTTCCGGG ACATGGACGA TGAGGAGTCC TGGATCAAGG AGAAGAAGCT GCTGGTGAGC 
TCAGAGGACT ACGGCCGCGA CCTGACGGGC GTCCAGAACC TGAGGAAGAA GCACAAACGG 
CTGGAGGCAG AGCTGGCTGC CCACGAGCCG GCCATTCAGG GTGTCCTGGA CACTGGAAAG 
AAGCTGTCTG ATGACAACAC CATCGGGAAG GAGGAGATTC AGCAGCGCCT GGCGCAGTTT 
GTGGAGCACT GGAAGGAGCT GAAGCAGCTG GCAGCTGCTC GGGGCCAGCG GCTAGAAGAA 
TCTTTGGAAT ATCAGCAGTT TGTGGCCAAC GTGGAGGAGG AGGAAGCCTG GATCAATGAG 
AAGATGACCC TGGTGGCCAG TGAGGATTAC GGAGACACTC TTGCTGCCAT CCAGGGCTTG 
CTGAAGAAAC ACGAAGCCTT CGAGACGGAC TTCACTGTCC ACAAGGACCG GGTGAATGAC 
GTCTGCACTA ACGGACAAGA CCTGGTTAAG AAGAACAATC ACCATGAGGA GAACATCTCT 
TCCAAGATGA AGGGCTTGAA CGGGAAAGTG TCAGACTTGG AGAAGGCTGC CTCCCAGAGG 
AAGGCAAAGC TCGACGAGAA CTCGGCCTTC CTCCAGTTCA ACTGGAAGGC CGATGTGGTG 
GAGTCCTGGA TTGGTGAAAA GGAGAACAGC TTGAAGACAG ATGATTATGG TCGAGATCTG 
TCTTCTGTCC AAACTCTCCT CACCAAACAG GAAACCTTCG ATGCTGGCCT GCAGGCCTTC 
CAGCAGGAGG GCATCGCCAA CATCACTGCC CTCAAAGATC AGCTGCTGGC CGCCAAGCAT 
GTTCAGTCCA AGGCCATCGA GGCCCGCCAC GCTGCCCTCA TGAAGAGATG GAGCCAGCTC 
CTGGCCAATT CGGCCACCCG GAAGAAGAAG CTGCTGGAGG CACAGAGTCA CTTCCGCAAG 
GTGGAGGATC TCTTCCTGAC CTTTGCCAAA AAGGCTTCAG CCTTCAACAG CTGGTTTGAA 
AATGCAGAGG AGGACTTAAC AGACCCAGTG CGCTGCAACT CCCTGGAAGA GATCAAGGCC 
TTGAGAGAGG CCCACGATGC CTTCCGCTCC TCGCTGAGCT CAGCCCAGGC CGACTTCAAC 
CAGCTGGCTG AGCTGGACCG CCAGATCAAG AGCTTCCGCG TGGCCTCCAA CCCCTACACC 
TGGTTCACCA TGGAGGCCCT GGAGGAGACC TGGAGGAACC TTCAGAAGAT CATCAAGGAA 
AGGGAGCTGG AGCTGCAGAA GGAGCAGCGG CGGCAGGAGG AGAACGACAA GCTGCGCCAG 
GAGTTCGCCC AGCATGCCAA CGCCTTCCAC CAGTGGATCC AGGAGACCAG AACGTATCTC 
CTCGATGGCA TAGCGTATCG TCGGGTCATT CGTGTCTATC AGTATGAAGT TGGGGATGAT 
CTGTCTGGAA GGTCCTGCAT GGTGGAAGAA TCTGGGACCC TTGAATCGCA GCTTGAAGCT 
ACCAAACGCA AACACCAGGA GATTCGAGCC ATGAGAAGTC AGCTCAAGAA GATTGAAGAC 
CTGGGGGCGG CCATGGAGGA AGCGCTGATC CTGGACAACA AGTACACGGA GCACAGCACC 
GTGGGCCTGG CCCAGCAGTG GGACCAGCTC GACCAGCTGG GCATGCGGAT GCAGCACAAC 
CTGGAGCAGC AGATCCAGGC CAGGAACACG ACAGGAGTGA CCGAGGAGGC CCTCAAAGAA 
TTCAGCATGA TGTTCAAACA CTTCGACAAG GACAAGTCCG GCCGGCTGAA TCACCAGGAG 
TTCAAATCCT GTCTGCGCTC CCTGGGCTAT GACCTGCCCA TGGTGGAGGA AGGGGAGCCC 
GACCCTGAGT TCGAGGCCAT CTTGGATACT GTGGATCCTA ACAGGGACGG CCACGTCTCC 
CTGCAAGAGT ACATGGCCTT TATGATCAGC CGTGAGACCG AGAATGTCAA GTCCAGCGAG 
GAGATTGAGA GCGCCTTCCG GGCCCTGAGC TCCGAAGGGA AGCCTTATGT GACCAAGGAG 
GAGCTGTACC AGAACCTGAC TCGGGAACAA GCCGACTACT GCGTCTCCCA CATGAAGCCC 
TACGTGGACG GCAAGGGCCG CGAACTCCCC ACTGCTTTCG ACTACGTTGA GTTCACCCGC 
TCGCTCTTCG TGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_027822156.1
CDS	72..7589
Translation	MLSSFRKRRVQKMDPSGVKVLETAEDIQERRQQVLDRYHRFKELSTLRRQKLEDSYRFQFFQRDAEELEKWIQEKLQIASDENYKDPTNLQGKLQKHQAFEAEVQANSGAIVKLDETGNLMISEGHFASETIRTRLMELHRQWELLLEKMREKGIKLLQAQKLVQYLRECEDVMDWINDKEAIVTSEELGQDLEHVEVLQKKFEEFQTDMAAHEERVNEVNQFAAKLIQEQHPEEELIKTKEDEVNAAWQRLKGLALQRQGKLFGAAEVQRFNRDVDETISWIKEKEQLMASDDFGRDLASVQALLRKHEGLERDLAALEDKVKALCAEADRLQQSHPLSATQIQVKREELITNWEQIRTLAAERHARLNDSYRLQRFLADFRDLTSWVTEMKALINADELANDVAGAEALLDRHQEHKGEIDAHEDSFKSADESGQALLAAGHYASDEVREKLTILSEERAALLELWELRRQQYEQCMDLQLFYRDTEQVDNWMSKQEAFLLNEDLGDSLDSVEALLKKHEDFEKSLSAQEEKITALDEFATKLIQNNHYAMEDVATRRDALLSRRNALHERAMYRRAQLADSFHLQQFFRDSDELKSWVNEKMKTATDEAYKDPSNLQGKVQKHQAFEAELSANQSRIDALEKAGQKLIDVDHYAKDEVATRMNEVISLWKKLLEATELKGIKLREANQQQQFNRNVEDIELWLYEVEGHLASDDYGKDLTNVQNLQKKHALLEADVAAHQDRIDGITIQARQFQDAGHFDAENIKKKQEALVARYEALKEPMVARKQKLADSLRLQQLFRDVEDEETWIREKEPIAASTNRGKDLIGVQNLLKKHQALQAEIAGHEPRIKAVTQKGNAMVEEGHFAAEDVKAKLHELNQKWESLKSKASQRRQDLEDSLQAQQYFADANEAESWMREKEPIVGSTDYGKDEDSAEALLKKHEALMSDLSAYGSSIQALREQAQSCRQQVAPMDDETGKELVLALYDYQEKSPREVTMKKGDILTLLNSTNKDWWKVEVNDRQGFVPAAYVKKLDPAQSASRENLLEEQGSIALRQEQIDNQTRVTKEAGSVSLRMKQVEELYHSLLELGEKRKGMLEKSCKKFMLFREANELQQWINEKEAALTSEEVGADLEQVEVLQKKFDDFQKDLKANESRLKDINKVAEDLESEGLMAEEVQAVQQQEVYGAMPRDESDSKTASPWKSARLMVHTVATFNSIKELNERWRSLQQLAEERSQLLGSAHEVQRFHRDADETKEWIEEKNQALNTDNYGHDLASVQALQRKHEGFERDLAALGDKVNSLGETAERLIQSHPESAEDLQEKCTELNQAWSSLGKRADQRKAKLGDSHDLQRFLSDFRDLMSWINGIRGLVSSDELAKDVTGAEALLERHQEHRTEIDARAGTFQAFEQFGQQLLAHGHYASPEIKEKLDVLDQERADLEKAWVQRRMMLDQCLELQLFHRDCEQAENWMAAREAFLNTEDKGDSLDSVEALIKKHEDFDKAINVQEEKIAALQSFADQLIAGGHYAKGDISSRRNEVLDRWRRLKAQMIEKRSKLGESQTLQQFSRDVDEIEAWISEKLQTASDESYKDPTNIQSKHQKHQAFEAELHANADRIRGVIDVGNSLIDRGACAGSEDAVKARLAALADQWQFLVQKSAEKSQKLKEANKQQNFNTGIKDFDFWLSEVEALLASEDYGKDLASVNNLLKKHQLLEADISAHEDRLKDLNSQADSLMTSSAFDTSQVKDKRDTINSRFQKIKSMAASRRARLNESHRLHQFFRDMDDEESWIKEKKLLVSSEDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQFVEHWKELKQLAAARGQRLEESLEYQQFVANVEEEEAWINEKMTLVASEDYGDTLAAIQGLLKKHEAFETDFTVHKDRVNDVCTNGQDLVKKNNHHEENISSKMKGLNGKVSDLEKAASQRKAKLDENSAFLQFNWKADVVESWIGEKENSLKTDDYGRDLSSVQTLLTKQETFDAGLQAFQQEGIANITALKDQLLAAKHVQSKAIEARHAALMKRWSQLLANSATRKKKLLEAQSHFRKVEDLFLTFAKKASAFNSWFENAEEDLTDPVRCNSLEEIKALREAHDAFRSSLSSAQADFNQLAELDRQIKSFRVASNPYTWFTMEALEETWRNLQKIIKERELELQKEQRRQEENDKLRQEFAQHANAFHQWIQETRTYLLDGIAYRRVIRVYQYEVGDDLSGRSCMVEESGTLESQLEATKRKHQEIRAMRSQLKKIEDLGAAMEEALILDNKYTEHSTVGLAQQWDQLDQLGMRMQHNLEQQIQARNTTGVTEEALKEFSMMFKHFDKDKSGRLNHQEFKSCLRSLGYDLPMVEEGEPDPEFEAILDTVDPNRDGHVSLQEYMAFMISRETENVKSSEEIESAFRALSSEGKPYVTKEELYQNLTREQADYCVSHMKPYVDGKGRELPTAFDYVEFTRSLFVN

Target ORF information:

RefSeq Version	XM_027966355.1
Organism	Ovis aries(sheep)
Definition	Ovis aries spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027966355.1

ORF Insert Sequence:

ATGCTGTCAT CGTTTCGTAA ACGCAGAGTC CAGAAAATGG ACCCAAGCGG GGTCAAAGTG 
CTGGAGACAG CCGAGGACAT CCAGGAGAGG CGGCAGCAGG TCCTGGACAG ATACCACCGC 
TTCAAGGAGC TCTCGACCCT GAGGCGCCAG AAGCTTGAAG ACTCCTACCG ATTCCAGTTT 
TTCCAAAGAG ATGCTGAGGA GCTGGAGAAA TGGATTCAGG AGAAGCTTCA GATTGCCTCG 
GATGAGAATT ACAAAGACCC AACCAACCTG CAGGGCAAGC TGCAGAAGCA TCAAGCCTTC 
GAAGCCGAGG TGCAGGCCAA CTCGGGTGCC ATCGTGAAGC TTGATGAGAC CGGTAACCTG 
ATGATCTCAG AAGGGCACTT CGCGTCCGAA ACCATCCGGA CTCGTCTGAT GGAGCTGCAC 
CGCCAGTGGG AATTGCTTCT GGAGAAGATG CGGGAGAAAG GCATCAAGCT GCTGCAGGCC 
CAGAAGCTGG TGCAGTACCT GCGGGAGTGT GAGGACGTGA TGGACTGGAT CAATGACAAG 
GAAGCCATTG TGACATCTGA GGAGCTGGGC CAGGACCTGG AGCACGTGGA GGTGTTACAG 
AAGAAATTCG AAGAGTTTCA GACAGACATG GCTGCCCACG AAGAGAGAGT CAATGAAGTG 
AACCAGTTTG CTGCCAAGCT CATTCAGGAG CAGCACCCTG AGGAGGAGCT GATCAAGACA 
AAGGAGGACG AGGTGAATGC AGCCTGGCAG CGGCTGAAGG GCCTGGCCCT CCAGCGGCAG 
GGGAAGCTGT TCGGGGCAGC TGAGGTCCAA CGCTTTAACA GGGATGTGGA CGAGACCATT 
AGCTGGATTA AGGAGAAGGA ACAGTTAATG GCCTCTGATG ACTTTGGCCG GGACCTGGCC 
AGTGTCCAGG CTCTACTGCG GAAACACGAG GGTTTGGAGA GAGATCTTGC TGCCTTGGAG 
GACAAGGTCA AAGCCCTGTG TGCCGAGGCT GACCGCCTGC AGCAGTCCCA CCCTCTGAGT 
GCCACCCAGA TCCAAGTGAA GCGAGAGGAG CTGATCACCA ACTGGGAGCA GATCCGCACC 
CTGGCCGCGG AGAGACACGC ACGTCTCAAC GATTCCTACA GGCTTCAGCG CTTCCTTGCT 
GACTTCCGAG ACCTCACCAG CTGGGTCACT GAGATGAAAG CCCTCATCAA TGCAGATGAA 
CTTGCCAATG ATGTGGCCGG GGCCGAAGCC CTGCTGGATA GGCATCAGGA GCACAAGGGT 
GAAATTGACG CTCATGAAGA TAGCTTTAAA TCTGCAGATG AGTCTGGACA GGCCCTGCTT 
GCAGCTGGTC ACTATGCCTC AGATGAAGTG AGGGAGAAGC TGACCATCCT GTCCGAGGAG 
CGGGCCGCGC TACTAGAGCT CTGGGAGCTG CGACGGCAGC AATATGAGCA GTGCATGGAC 
CTGCAGCTCT TCTACCGCGA CACGGAGCAG GTGGACAACT GGATGAGCAA ACAGGAGGCG 
TTCCTGTTGA ACGAAGACCT GGGTGATTCC TTGGATAGTG TGGAAGCGCT TCTCAAGAAG 
CACGAAGACT TTGAGAAGTC CCTCAGCGCC CAGGAGGAGA AGATCACAGC CTTAGATGAG 
TTTGCCACCA AGCTGATTCA GAACAACCAC TACGCGATGG AAGATGTGGC CACCCGGCGA 
GACGCACTGC TGAGCCGCCG CAACGCCCTC CATGAGAGAG CCATGTACCG CCGTGCCCAG 
CTTGCAGATT CCTTCCACTT GCAGCAGTTT TTCCGTGACT CTGATGAGCT CAAGAGTTGG 
GTCAACGAGA AGATGAAAAC AGCCACAGAT GAAGCTTATA AAGACCCATC CAACCTACAA 
GGGAAAGTGC AGAAGCACCA GGCCTTTGAG GCCGAGCTCT CAGCAAACCA GAGCCGCATC 
GACGCCTTGG AGAAAGCTGG GCAGAAGCTG ATCGACGTTG ACCACTATGC CAAGGATGAG 
GTGGCGACTC GGATGAATGA AGTTATCAGT CTGTGGAAGA AGCTGCTGGA GGCCACGGAG 
CTGAAAGGCA TAAAGCTCCG TGAGGCTAAC CAGCAGCAGC AGTTTAACCG CAACGTTGAG 
GACATCGAGC TGTGGCTGTA CGAAGTGGAA GGTCATCTGG CCTCGGATGA CTACGGCAAA 
GACCTCACCA ACGTCCAGAA CCTCCAGAAA AAGCATGCCC TGCTGGAGGC TGACGTGGCC 
GCTCACCAGG ATCGCATCGA TGGCATCACT ATCCAAGCCC GCCAGTTCCA GGATGCGGGC 
CACTTTGATG CTGAAAACAT CAAGAAGAAG CAGGAGGCCC TGGTAGCTCG CTACGAGGCA 
CTCAAGGAGC CCATGGTTGC CCGGAAGCAG AAGCTGGCCG ATTCCCTGCG GTTGCAGCAG 
CTCTTCCGTG ACGTTGAGGA TGAGGAGACG TGGATTCGGG AGAAAGAACC TATTGCCGCC 
TCCACCAACA GAGGCAAAGA TTTAATTGGA GTCCAGAACC TGCTAAAGAA ACACCAAGCG 
CTACAAGCAG AAATTGCTGG CCACGAGCCC CGCATCAAAG CAGTCACACA GAAGGGGAAC 
GCCATGGTAG AGGAGGGCCA TTTTGCTGCC GAGGACGTGA AGGCCAAGCT CCATGAACTG 
AACCAGAAGT GGGAGTCGCT GAAGTCCAAG GCCTCCCAGC GACGGCAGGA CCTGGAGGAC 
TCGCTGCAGG CCCAGCAGTA CTTCGCTGAT GCCAACGAGG CCGAGTCCTG GATGCGGGAG 
AAGGAGCCCA TCGTGGGCAG CACCGACTAT GGCAAAGATG AGGACTCTGC CGAGGCTCTG 
CTAAAGAAGC ACGAGGCATT GATGTCGGAT CTCAGCGCCT ATGGCAGCAG CATCCAGGCC 
CTGCGAGAGC AGGCCCAGTC GTGCCGGCAA CAAGTGGCCC CCATGGATGA CGAGACTGGG 
AAGGAGCTGG TCTTGGCGCT CTATGACTAT CAGGAGAAGA GTCCCCGAGA GGTCACCATG 
AAGAAAGGAG ACATTCTCAC CTTACTCAAC AGCACCAACA AGGACTGGTG GAAAGTGGAA 
GTGAACGACC GGCAGGGCTT CGTGCCGGCC GCCTACGTGA AGAAGCTGGA CCCAGCGCAG 
TCGGCCTCCC GGGAGAACCT CCTGGAAGAG CAGGGCAGCA TCGCGCTGCG GCAGGAGCAG 
ATTGACAACC AGACGCGCGT AACTAAGGAG GCCGGCAGCG TGTCTCTGCG TATGAAACAG 
GTGGAAGAAC TGTACCATTC TCTGCTGGAA CTGGGAGAGA AGCGTAAAGG CATGTTGGAG 
AAGAGCTGTA AGAAGTTCAT GCTGTTCCGT GAAGCGAACG AGCTGCAGCA GTGGATCAAC 
GAGAAGGAAG CGGCCCTGAC AAGCGAGGAG GTCGGCGCGG ACTTGGAGCA GGTCGAAGTG 
CTGCAGAAGA AGTTTGACGA CTTCCAGAAG GATCTCAAGG CCAATGAGTC ACGGCTGAAG 
GACATTAACA AGGTGGCTGA AGACCTGGAG TCGGAAGGCC TGATGGCGGA GGAGGTGCAG 
GCCGTGCAGC AGCAGGAGGT GTATGGCGCG ATGCCCAGGG ATGAATCTGA TTCCAAGACA 
GCCTCCCCGT GGAAGTCTGC TCGCCTGATG GTTCACACCG TGGCCACCTT TAATTCCATC 
AAGGAGCTCA ATGAGCGCTG GCGGTCCCTG CAGCAGCTGG CTGAGGAGCG GAGCCAACTC 
TTGGGCAGCG CCCACGAAGT GCAGCGGTTC CACAGAGATG CTGACGAAAC CAAAGAGTGG 
ATCGAGGAGA AGAACCAGGC TCTAAACACA GACAACTATG GCCACGACCT GGCCAGCGTC 
CAGGCCCTGC AGCGCAAGCA TGAGGGCTTC GAGAGGGACC TCGCGGCCCT TGGCGACAAG 
GTGAACTCGC TCGGGGAGAC AGCCGAGCGC CTGATCCAGT CCCACCCCGA GTCGGCAGAG 
GACCTGCAGG AGAAGTGCAC TGAGCTGAAC CAGGCATGGA GCAGCCTGGG CAAGCGTGCC 
GACCAGCGCA AGGCCAAGCT GGGCGACTCC CACGACCTGC AGCGCTTCCT CAGCGACTTC 
CGGGACCTCA TGTCTTGGAT CAATGGAATC CGAGGTCTGG TGTCCTCAGA CGAGCTAGCC 
AAGGATGTCA CTGGAGCTGA GGCCCTGCTG GAGCGACACC AGGAACACCG GACAGAAATT 
GATGCCAGAG CTGGCACTTT CCAGGCATTC GAGCAGTTTG GGCAGCAGCT GTTGGCTCAT 
GGGCACTACG CCAGCCCCGA GATCAAGGAG AAGCTCGATG TTCTAGACCA GGAGCGCGCA 
GACCTCGAGA AGGCCTGGGT CCAGCGCAGG ATGATGCTGG ATCAGTGCCT TGAATTGCAG 
CTGTTCCATC GGGATTGCGA GCAGGCCGAG AACTGGATGG CTGCCCGGGA GGCCTTCCTG 
AACACAGAAG ACAAAGGGGA CTCGCTAGAC AGCGTGGAGG CTCTGATCAA AAAACACGAG 
GACTTTGACA AAGCAATTAA TGTCCAGGAG GAGAAGATTG CTGCCCTGCA GTCCTTTGCT 
GACCAGCTCA TTGCTGGCGG TCACTACGCC AAAGGCGACA TCTCCAGCCG GCGCAATGAG 
GTTTTGGACA GGTGGCGCCG TCTAAAAGCT CAGATGATTG AGAAGAGGTC AAAGCTGGGA 
GAATCGCAGA CCCTCCAGCA GTTCAGCCGG GATGTAGACG AGATTGAAGC TTGGATCAGT 
GAAAAATTAC AGACTGCAAG TGATGAGTCA TATAAGGACC CCACCAACAT CCAGAGCAAG 
CATCAGAAGC ACCAGGCCTT CGAGGCAGAA CTGCACGCCA ATGCGGACCG GATCCGCGGG 
GTCATCGACG TGGGCAACTC CCTCATTGAC CGTGGGGCCT GTGCCGGCAG CGAGGATGCC 
GTCAAGGCCC GGCTGGCCGC CTTAGCTGAC CAGTGGCAGT TCCTGGTGCA GAAGTCGGCT 
GAGAAGAGCC AGAAGCTGAA AGAAGCCAAC AAACAGCAGA ACTTCAACAC TGGCATCAAG 
GACTTCGACT TCTGGCTCTC TGAGGTGGAA GCCCTGCTGG CCTCTGAGGA TTATGGCAAA 
GACCTGGCTT CTGTGAACAA CTTGCTAAAA AAACATCAGC TGCTGGAGGC AGATATCTCT 
GCCCACGAGG ACCGCCTGAA GGACCTCAAC AGCCAGGCGG ACAGCCTGAT GACCAGCAGC 
GCCTTCGACA CCTCCCAGGT GAAGGATAAG CGGGACACCA TCAACAGCCG TTTCCAGAAG 
ATCAAGAGCA TGGCCGCCTC CCGGCGAGCT AGGCTGAATG AGTCCCACCG CCTGCACCAG 
TTCTTCCGGG ACATGGACGA TGAGGAGTCC TGGATCAAGG AGAAGAAGCT GCTGGTGAGC 
TCAGAGGACT ACGGCCGCGA CCTGACGGGC GTCCAGAACC TGAGGAAGAA GCACAAACGG 
CTGGAGGCAG AGCTGGCTGC CCACGAGCCG GCCATTCAGG GTGTCCTGGA CACTGGAAAG 
AAGCTGTCTG ATGACAACAC CATCGGGAAG GAGGAGATTC AGCAGCGCCT GGCGCAGTTT 
GTGGAGCACT GGAAGGAGCT GAAGCAGCTG GCAGCTGCTC GGGGCCAGCG GCTAGAAGAA 
TCTTTGGAAT ATCAGCAGTT TGTGGCCAAC GTGGAGGAGG AGGAAGCCTG GATCAATGAG 
AAGATGACCC TGGTGGCCAG TGAGGATTAC GGAGACACTC TTGCTGCCAT CCAGGGCTTG 
CTGAAGAAAC ACGAAGCCTT CGAGACGGAC TTCACTGTCC ACAAGGACCG GGTGAATGAC 
GTCTGCACTA ACGGACAAGA CCTGGTTAAG AAGAACAATC ACCATGAGGA GAACATCTCT 
TCCAAGATGA AGGGCTTGAA CGGGAAAGTG TCAGACTTGG AGAAGGCTGC CTCCCAGAGG 
AAGGCAAAGC TCGACGAGAA CTCGGCCTTC CTCCAGTTCA ACTGGAAGGC CGATGTGGTG 
GAGTCCTGGA TTGGTGAAAA GGAGAACAGC TTGAAGACAG ATGATTATGG TCGAGATCTG 
TCTTCTGTCC AAACTCTCCT CACCAAACAG GAAACCTTCG ATGCTGGCCT GCAGGCCTTC 
CAGCAGGAGG GCATCGCCAA CATCACTGCC CTCAAAGATC AGCTGCTGGC CGCCAAGCAT 
GTTCAGTCCA AGGCCATCGA GGCCCGCCAC GCTGCCCTCA TGAAGAGATG GAGCCAGCTC 
CTGGCCAATT CGGCCACCCG GAAGAAGAAG CTGCTGGAGG CACAGAGTCA CTTCCGCAAG 
GTGGAGGATC TCTTCCTGAC CTTTGCCAAA AAGGCTTCAG CCTTCAACAG CTGGTTTGAA 
AATGCAGAGG AGGACTTAAC AGACCCAGTG CGCTGCAACT CCCTGGAAGA GATCAAGGCC 
TTGAGAGAGG CCCACGATGC CTTCCGCTCC TCGCTGAGCT CAGCCCAGGC CGACTTCAAC 
CAGCTGGCTG AGCTGGACCG CCAGATCAAG AGCTTCCGCG TGGCCTCCAA CCCCTACACC 
TGGTTCACCA TGGAGGCCCT GGAGGAGACC TGGAGGAACC TTCAGAAGAT CATCAAGGAA 
AGGGAGCTGG AGCTGCAGAA GGAGCAGCGG CGGCAGGAGG AGAACGACAA GCTGCGCCAG 
GAGTTCGCCC AGCATGCCAA CGCCTTCCAC CAGTGGATCC AGGAGACCAG AACGTATCTC 
CTCGATGGCA TAGCGTATCG TCGGGTCATT CGTGTCTATC AGTATGAAGT TGGGGATGAT 
CTGTCTGGAA GGTCCTGCAT GGTGGAAGAA TCTGGGACCC TTGAATCGCA GCTTGAAGCT 
ACCAAACGCA AACACCAGGA GATTCGAGCC ATGAGAAGTC AGCTCAAGAA GATTGAAGAC 
CTGGGGGCGG CCATGGAGGA AGCGCTGATC CTGGACAACA AGTACACGGA GCACAGCACC 
GTGGGCCTGG CCCAGCAGTG GGACCAGCTC GACCAGCTGG GCATGCGGAT GCAGCACAAC 
CTGGAGCAGC AGATCCAGGC CAGGAACACG ACAGGAGTGA CCGAGGAGGC CCTCAAAGAA 
TTCAGCATGA TGTTCAAACA CTTCGACAAG GACAAGTCCG GCCGGCTGAA TCACCAGGAG 
TTCAAATCCT GTCTGCGCTC CCTGGGCTAT GACCTGCCCA TGGTGGAGGA AGGGGAGCCC 
GACCCTGAGT TCGAGGCCAT CTTGGATACT GTGGATCCTA ACAGGGACGG CCACGTCTCC 
CTGCAAGAGT ACATGGCCTT TATGATCAGC CGTGAGACCG AGAATGTCAA GTCCAGCGAG 
GAGATTGAGA GCGCCTTCCG GGCCCTGAGC TCCGAAGGGA AGCCTTATGT GACCAAGGAG 
GAGCTGTACC AGAACCTGAC TCGGGAACAA GCCGACTACT GCGTCTCCCA CATGAAGCCC 
TACGTGGACG GCAAGGGCCG CGAACTCCCC ACTGCTTTCG ACTACGTTGA GTTCACCCGC 
TCGCTCTTCG TGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTAN1 ( XM_027966356.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_027822157.1 Ovis aries spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X3, mRNA.

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CloneID	OSm76650
Clone ID Related Accession (Same CDS sequence)	XM_027966356.1
Accession Version	XM_027966356.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7497bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-05
Organism	Ovis aries(sheep)
Product	spectrin alpha chain, non-erythrocytic 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040254.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ovis aries Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCCAA GCGGGGTCAA AGTGCTGGAG ACAGCCGAGG ACATCCAGGA GAGGCGGCAG 
CAGGTCCTGG ACAGATACCA CCGCTTCAAG GAGCTCTCGA CCCTGAGGCG CCAGAAGCTT 
GAAGACTCCT ACCGATTCCA GTTTTTCCAA AGAGATGCTG AGGAGCTGGA GAAATGGATT 
CAGGAGAAGC TTCAGATTGC CTCGGATGAG AATTACAAAG ACCCAACCAA CCTGCAGGGC 
AAGCTGCAGA AGCATCAAGC CTTCGAAGCC GAGGTGCAGG CCAACTCGGG TGCCATCGTG 
AAGCTTGATG AGACCGGTAA CCTGATGATC TCAGAAGGGC ACTTCGCGTC CGAAACCATC 
CGGACTCGTC TGATGGAGCT GCACCGCCAG TGGGAATTGC TTCTGGAGAA GATGCGGGAG 
AAAGGCATCA AGCTGCTGCA GGCCCAGAAG CTGGTGCAGT ACCTGCGGGA GTGTGAGGAC 
GTGATGGACT GGATCAATGA CAAGGAAGCC ATTGTGACAT CTGAGGAGCT GGGCCAGGAC 
CTGGAGCACG TGGAGGTGTT ACAGAAGAAA TTCGAAGAGT TTCAGACAGA CATGGCTGCC 
CACGAAGAGA GAGTCAATGA AGTGAACCAG TTTGCTGCCA AGCTCATTCA GGAGCAGCAC 
CCTGAGGAGG AGCTGATCAA GACAAAGGAG GACGAGGTGA ATGCAGCCTG GCAGCGGCTG 
AAGGGCCTGG CCCTCCAGCG GCAGGGGAAG CTGTTCGGGG CAGCTGAGGT CCAACGCTTT 
AACAGGGATG TGGACGAGAC CATTAGCTGG ATTAAGGAGA AGGAACAGTT AATGGCCTCT 
GATGACTTTG GCCGGGACCT GGCCAGTGTC CAGGCTCTAC TGCGGAAACA CGAGGGTTTG 
GAGAGAGATC TTGCTGCCTT GGAGGACAAG GTCAAAGCCC TGTGTGCCGA GGCTGACCGC 
CTGCAGCAGT CCCACCCTCT GAGTGCCACC CAGATCCAAG TGAAGCGAGA GGAGCTGATC 
ACCAACTGGG AGCAGATCCG CACCCTGGCC GCGGAGAGAC ACGCACGTCT CAACGATTCC 
TACAGGCTTC AGCGCTTCCT TGCTGACTTC CGAGACCTCA CCAGCTGGGT CACTGAGATG 
AAAGCCCTCA TCAATGCAGA TGAACTTGCC AATGATGTGG CCGGGGCCGA AGCCCTGCTG 
GATAGGCATC AGGAGCACAA GGGTGAAATT GACGCTCATG AAGATAGCTT TAAATCTGCA 
GATGAGTCTG GACAGGCCCT GCTTGCAGCT GGTCACTATG CCTCAGATGA AGTGAGGGAG 
AAGCTGACCA TCCTGTCCGA GGAGCGGGCC GCGCTACTAG AGCTCTGGGA GCTGCGACGG 
CAGCAATATG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GCGACACGGA GCAGGTGGAC 
AACTGGATGA GCAAACAGGA GGCGTTCCTG TTGAACGAAG ACCTGGGTGA TTCCTTGGAT 
AGTGTGGAAG CGCTTCTCAA GAAGCACGAA GACTTTGAGA AGTCCCTCAG CGCCCAGGAG 
GAGAAGATCA CAGCCTTAGA TGAGTTTGCC ACCAAGCTGA TTCAGAACAA CCACTACGCG 
ATGGAAGATG TGGCCACCCG GCGAGACGCA CTGCTGAGCC GCCGCAACGC CCTCCATGAG 
AGAGCCATGT ACCGCCGTGC CCAGCTTGCA GATTCCTTCC ACTTGCAGCA GTTTTTCCGT 
GACTCTGATG AGCTCAAGAG TTGGGTCAAC GAGAAGATGA AAACAGCCAC AGATGAAGCT 
TATAAAGACC CATCCAACCT ACAAGGGAAA GTGCAGAAGC ACCAGGCCTT TGAGGCCGAG 
CTCTCAGCAA ACCAGAGCCG CATCGACGCC TTGGAGAAAG CTGGGCAGAA GCTGATCGAC 
GTTGACCACT ATGCCAAGGA TGAGGTGGCG ACTCGGATGA ATGAAGTTAT CAGTCTGTGG 
AAGAAGCTGC TGGAGGCCAC GGAGCTGAAA GGCATAAAGC TCCGTGAGGC TAACCAGCAG 
CAGCAGTTTA ACCGCAACGT TGAGGACATC GAGCTGTGGC TGTACGAAGT GGAAGGTCAT 
CTGGCCTCGG ATGACTACGG CAAAGACCTC ACCAACGTCC AGAACCTCCA GAAAAAGCAT 
GCCCTGCTGG AGGCTGACGT GGCCGCTCAC CAGGATCGCA TCGATGGCAT CACTATCCAA 
GCCCGCCAGT TCCAGGATGC GGGCCACTTT GATGCTGAAA ACATCAAGAA GAAGCAGGAG 
GCCCTGGTAG CTCGCTACGA GGCACTCAAG GAGCCCATGG TTGCCCGGAA GCAGAAGCTG 
GCCGATTCCC TGCGGTTGCA GCAGCTCTTC CGTGACGTTG AGGATGAGGA GACGTGGATT 
CGGGAGAAAG AACCTATTGC CGCCTCCACC AACAGAGGCA AAGATTTAAT TGGAGTCCAG 
AACCTGCTAA AGAAACACCA AGCGCTACAA GCAGAAATTG CTGGCCACGA GCCCCGCATC 
AAAGCAGTCA CACAGAAGGG GAACGCCATG GTAGAGGAGG GCCATTTTGC TGCCGAGGAC 
GTGAAGGCCA AGCTCCATGA ACTGAACCAG AAGTGGGAGT CGCTGAAGTC CAAGGCCTCC 
CAGCGACGGC AGGACCTGGA GGACTCGCTG CAGGCCCAGC AGTACTTCGC TGATGCCAAC 
GAGGCCGAGT CCTGGATGCG GGAGAAGGAG CCCATCGTGG GCAGCACCGA CTATGGCAAA 
GATGAGGACT CTGCCGAGGC TCTGCTAAAG AAGCACGAGG CATTGATGTC GGATCTCAGC 
GCCTATGGCA GCAGCATCCA GGCCCTGCGA GAGCAGGCCC AGTCGTGCCG GCAACAAGTG 
GCCCCCATGG ATGACGAGAC TGGGAAGGAG CTGGTCTTGG CGCTCTATGA CTATCAGGAG 
AAGAGTCCCC GAGAGGTCAC CATGAAGAAA GGAGACATTC TCACCTTACT CAACAGCACC 
AACAAGGACT GGTGGAAAGT GGAAGTGAAC GACCGGCAGG GCTTCGTGCC GGCCGCCTAC 
GTGAAGAAGC TGGACCCAGC GCAGTCGGCC TCCCGGGAGA ACCTCCTGGA AGAGCAGGGC 
AGCATCGCGC TGCGGCAGGA GCAGATTGAC AACCAGACGC GCGTAACTAA GGAGGCCGGC 
AGCGTGTCTC TGCGTATGAA ACAGGTGGAA GAACTGTACC ATTCTCTGCT GGAACTGGGA 
GAGAAGCGTA AAGGCATGTT GGAGAAGAGC TGTAAGAAGT TCATGCTGTT CCGTGAAGCG 
AACGAGCTGC AGCAGTGGAT CAACGAGAAG GAAGCGGCCC TGACAAGCGA GGAGGTCGGC 
GCGGACTTGG AGCAGGTCGA AGTGCTGCAG AAGAAGTTTG ACGACTTCCA GAAGGATCTC 
AAGGCCAATG AGTCACGGCT GAAGGACATT AACAAGGTGG CTGAAGACCT GGAGTCGGAA 
GGCCTGATGG CGGAGGAGGT GCAGGCCGTG CAGCAGCAGG AGGTGTATGG CGCGATGCCC 
AGGGATGAAT CTGATTCCAA GACAGCCTCC CCGTGGAAGT CTGCTCGCCT GATGGTTCAC 
ACCGTGGCCA CCTTTAATTC CATCAAGGAG CTCAATGAGC GCTGGCGGTC CCTGCAGCAG 
CTGGCTGAGG AGCGGAGCCA ACTCTTGGGC AGCGCCCACG AAGTGCAGCG GTTCCACAGA 
GATGCTGACG AAACCAAAGA GTGGATCGAG GAGAAGAACC AGGCTCTAAA CACAGACAAC 
TATGGCCACG ACCTGGCCAG CGTCCAGGCC CTGCAGCGCA AGCATGAGGG CTTCGAGAGG 
GACCTCGCGG CCCTTGGCGA CAAGGTGAAC TCGCTCGGGG AGACAGCCGA GCGCCTGATC 
CAGTCCCACC CCGAGTCGGC AGAGGACCTG CAGGAGAAGT GCACTGAGCT GAACCAGGCA 
TGGAGCAGCC TGGGCAAGCG TGCCGACCAG CGCAAGGCCA AGCTGGGCGA CTCCCACGAC 
CTGCAGCGCT TCCTCAGCGA CTTCCGGGAC CTCATGTCTT GGATCAATGG AATCCGAGGT 
CTGGTGTCCT CAGACGAGCT AGCCAAGGAT GTCACTGGAG CTGAGGCCCT GCTGGAGCGA 
CACCAGGAAC ACCGGACAGA AATTGATGCC AGAGCTGGCA CTTTCCAGGC ATTCGAGCAG 
TTTGGGCAGC AGCTGTTGGC TCATGGGCAC TACGCCAGCC CCGAGATCAA GGAGAAGCTC 
GATGTTCTAG ACCAGGAGCG CGCAGACCTC GAGAAGGCCT GGGTCCAGCG CAGGATGATG 
CTGGATCAGT GCCTTGAATT GCAGCTGTTC CATCGGGATT GCGAGCAGGC CGAGAACTGG 
ATGGCTGCCC GGGAGGCCTT CCTGAACACA GAAGACAAAG GGGACTCGCT AGACAGCGTG 
GAGGCTCTGA TCAAAAAACA CGAGGACTTT GACAAAGCAA TTAATGTCCA GGAGGAGAAG 
ATTGCTGCCC TGCAGTCCTT TGCTGACCAG CTCATTGCTG GCGGTCACTA CGCCAAAGGC 
GACATCTCCA GCCGGCGCAA TGAGGTTTTG GACAGGTGGC GCCGTCTAAA AGCTCAGATG 
ATTGAGAAGA GGTCAAAGCT GGGAGAATCG CAGACCCTCC AGCAGTTCAG CCGGGATGTA 
GACGAGATTG AAGCTTGGAT CAGTGAAAAA TTACAGACTG CAAGTGATGA GTCATATAAG 
GACCCCACCA ACATCCAGCT TTCCAAGCTG CTGAGCAAGC ATCAGAAGCA CCAGGCCTTC 
GAGGCAGAAC TGCACGCCAA TGCGGACCGG ATCCGCGGGG TCATCGACGT GGGCAACTCC 
CTCATTGACC GTGGGGCCTG TGCCGGCAGC GAGGATGCCG TCAAGGCCCG GCTGGCCGCC 
TTAGCTGACC AGTGGCAGTT CCTGGTGCAG AAGTCGGCTG AGAAGAGCCA GAAGCTGAAA 
GAAGCCAACA AACAGCAGAA CTTCAACACT GGCATCAAGG ACTTCGACTT CTGGCTCTCT 
GAGGTGGAAG CCCTGCTGGC CTCTGAGGAT TATGGCAAAG ACCTGGCTTC TGTGAACAAC 
TTGCTAAAAA AACATCAGCT GCTGGAGGCA GATATCTCTG CCCACGAGGA CCGCCTGAAG 
GACCTCAACA GCCAGGCGGA CAGCCTGATG ACCAGCAGCG CCTTCGACAC CTCCCAGGTG 
AAGGATAAGC GGGACACCAT CAACAGCCGT TTCCAGAAGA TCAAGAGCAT GGCCGCCTCC 
CGGCGAGCTA GGCTGAATGA GTCCCACCGC CTGCACCAGT TCTTCCGGGA CATGGACGAT 
GAGGAGTCCT GGATCAAGGA GAAGAAGCTG CTGGTGAGCT CAGAGGACTA CGGCCGCGAC 
CTGACGGGCG TCCAGAACCT GAGGAAGAAG CACAAACGGC TGGAGGCAGA GCTGGCTGCC 
CACGAGCCGG CCATTCAGGG TGTCCTGGAC ACTGGAAAGA AGCTGTCTGA TGACAACACC 
ATCGGGAAGG AGGAGATTCA GCAGCGCCTG GCGCAGTTTG TGGAGCACTG GAAGGAGCTG 
AAGCAGCTGG CAGCTGCTCG GGGCCAGCGG CTAGAAGAAT CTTTGGAATA TCAGCAGTTT 
GTGGCCAACG TGGAGGAGGA GGAAGCCTGG ATCAATGAGA AGATGACCCT GGTGGCCAGT 
GAGGATTACG GAGACACTCT TGCTGCCATC CAGGGCTTGC TGAAGAAACA CGAAGCCTTC 
GAGACGGACT TCACTGTCCA CAAGGACCGG GTGAATGACG TCTGCACTAA CGGACAAGAC 
CTGGTTAAGA AGAACAATCA CCATGAGGAG AACATCTCTT CCAAGATGAA GGGCTTGAAC 
GGGAAAGTGT CAGACTTGGA GAAGGCTGCC TCCCAGAGGA AGGCAAAGCT CGACGAGAAC 
TCGGCCTTCC TCCAGTTCAA CTGGAAGGCC GATGTGGTGG AGTCCTGGAT TGGTGAAAAG 
GAGAACAGCT TGAAGACAGA TGATTATGGT CGAGATCTGT CTTCTGTCCA AACTCTCCTC 
ACCAAACAGG AAACCTTCGA TGCTGGCCTG CAGGCCTTCC AGCAGGAGGG CATCGCCAAC 
ATCACTGCCC TCAAAGATCA GCTGCTGGCC GCCAAGCATG TTCAGTCCAA GGCCATCGAG 
GCCCGCCACG CTGCCCTCAT GAAGAGATGG AGCCAGCTCC TGGCCAATTC GGCCACCCGG 
AAGAAGAAGC TGCTGGAGGC ACAGAGTCAC TTCCGCAAGG TGGAGGATCT CTTCCTGACC 
TTTGCCAAAA AGGCTTCAGC CTTCAACAGC TGGTTTGAAA ATGCAGAGGA GGACTTAACA 
GACCCAGTGC GCTGCAACTC CCTGGAAGAG ATCAAGGCCT TGAGAGAGGC CCACGATGCC 
TTCCGCTCCT CGCTGAGCTC AGCCCAGGCC GACTTCAACC AGCTGGCTGA GCTGGACCGC 
CAGATCAAGA GCTTCCGCGT GGCCTCCAAC CCCTACACCT GGTTCACCAT GGAGGCCCTG 
GAGGAGACCT GGAGGAACCT TCAGAAGATC ATCAAGGAAA GGGAGCTGGA GCTGCAGAAG 
GAGCAGCGGC GGCAGGAGGA GAACGACAAG CTGCGCCAGG AGTTCGCCCA GCATGCCAAC 
GCCTTCCACC AGTGGATCCA GGAGACCAGA ACGTATCTCC TCGATGGCAT AGCGTATCGT 
CGGGTCATTC GTGTCTATCA GTATGAAGTT GGGGATGATC TGTCTGGAAG GTCCTGCATG 
GTGGAAGAAT CTGGGACCCT TGAATCGCAG CTTGAAGCTA CCAAACGCAA ACACCAGGAG 
ATTCGAGCCA TGAGAAGTCA GCTCAAGAAG ATTGAAGACC TGGGGGCGGC CATGGAGGAA 
GCGCTGATCC TGGACAACAA GTACACGGAG CACAGCACCG TGGGCCTGGC CCAGCAGTGG 
GACCAGCTCG ACCAGCTGGG CATGCGGATG CAGCACAACC TGGAGCAGCA GATCCAGGCC 
AGGAACACGA CAGGAGTGAC CGAGGAGGCC CTCAAAGAAT TCAGCATGAT GTTCAAACAC 
TTCGACAAGG ACAAGTCCGG CCGGCTGAAT CACCAGGAGT TCAAATCCTG TCTGCGCTCC 
CTGGGCTATG ACCTGCCCAT GGTGGAGGAA GGGGAGCCCG ACCCTGAGTT CGAGGCCATC 
TTGGATACTG TGGATCCTAA CAGGGACGGC CACGTCTCCC TGCAAGAGTA CATGGCCTTT 
ATGATCAGCC GTGAGACCGA GAATGTCAAG TCCAGCGAGG AGATTGAGAG CGCCTTCCGG 
GCCCTGAGCT CCGAAGGGAA GCCTTATGTG ACCAAGGAGG AGCTGTACCA GAACCTGACT 
CGGGAACAAG CCGACTACTG CGTCTCCCAC ATGAAGCCCT ACGTGGACGG CAAGGGCCGC 
GAACTCCCCA CTGCTTTCGA CTACGTTGAG TTCACCCGCT CGCTCTTCGT GAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_027822157.1
CDS	93..7589
Translation	MDPSGVKVLETAEDIQERRQQVLDRYHRFKELSTLRRQKLEDSYRFQFFQRDAEELEKWIQEKLQIASDENYKDPTNLQGKLQKHQAFEAEVQANSGAIVKLDETGNLMISEGHFASETIRTRLMELHRQWELLLEKMREKGIKLLQAQKLVQYLRECEDVMDWINDKEAIVTSEELGQDLEHVEVLQKKFEEFQTDMAAHEERVNEVNQFAAKLIQEQHPEEELIKTKEDEVNAAWQRLKGLALQRQGKLFGAAEVQRFNRDVDETISWIKEKEQLMASDDFGRDLASVQALLRKHEGLERDLAALEDKVKALCAEADRLQQSHPLSATQIQVKREELITNWEQIRTLAAERHARLNDSYRLQRFLADFRDLTSWVTEMKALINADELANDVAGAEALLDRHQEHKGEIDAHEDSFKSADESGQALLAAGHYASDEVREKLTILSEERAALLELWELRRQQYEQCMDLQLFYRDTEQVDNWMSKQEAFLLNEDLGDSLDSVEALLKKHEDFEKSLSAQEEKITALDEFATKLIQNNHYAMEDVATRRDALLSRRNALHERAMYRRAQLADSFHLQQFFRDSDELKSWVNEKMKTATDEAYKDPSNLQGKVQKHQAFEAELSANQSRIDALEKAGQKLIDVDHYAKDEVATRMNEVISLWKKLLEATELKGIKLREANQQQQFNRNVEDIELWLYEVEGHLASDDYGKDLTNVQNLQKKHALLEADVAAHQDRIDGITIQARQFQDAGHFDAENIKKKQEALVARYEALKEPMVARKQKLADSLRLQQLFRDVEDEETWIREKEPIAASTNRGKDLIGVQNLLKKHQALQAEIAGHEPRIKAVTQKGNAMVEEGHFAAEDVKAKLHELNQKWESLKSKASQRRQDLEDSLQAQQYFADANEAESWMREKEPIVGSTDYGKDEDSAEALLKKHEALMSDLSAYGSSIQALREQAQSCRQQVAPMDDETGKELVLALYDYQEKSPREVTMKKGDILTLLNSTNKDWWKVEVNDRQGFVPAAYVKKLDPAQSASRENLLEEQGSIALRQEQIDNQTRVTKEAGSVSLRMKQVEELYHSLLELGEKRKGMLEKSCKKFMLFREANELQQWINEKEAALTSEEVGADLEQVEVLQKKFDDFQKDLKANESRLKDINKVAEDLESEGLMAEEVQAVQQQEVYGAMPRDESDSKTASPWKSARLMVHTVATFNSIKELNERWRSLQQLAEERSQLLGSAHEVQRFHRDADETKEWIEEKNQALNTDNYGHDLASVQALQRKHEGFERDLAALGDKVNSLGETAERLIQSHPESAEDLQEKCTELNQAWSSLGKRADQRKAKLGDSHDLQRFLSDFRDLMSWINGIRGLVSSDELAKDVTGAEALLERHQEHRTEIDARAGTFQAFEQFGQQLLAHGHYASPEIKEKLDVLDQERADLEKAWVQRRMMLDQCLELQLFHRDCEQAENWMAAREAFLNTEDKGDSLDSVEALIKKHEDFDKAINVQEEKIAALQSFADQLIAGGHYAKGDISSRRNEVLDRWRRLKAQMIEKRSKLGESQTLQQFSRDVDEIEAWISEKLQTASDESYKDPTNIQLSKLLSKHQKHQAFEAELHANADRIRGVIDVGNSLIDRGACAGSEDAVKARLAALADQWQFLVQKSAEKSQKLKEANKQQNFNTGIKDFDFWLSEVEALLASEDYGKDLASVNNLLKKHQLLEADISAHEDRLKDLNSQADSLMTSSAFDTSQVKDKRDTINSRFQKIKSMAASRRARLNESHRLHQFFRDMDDEESWIKEKKLLVSSEDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQFVEHWKELKQLAAARGQRLEESLEYQQFVANVEEEEAWINEKMTLVASEDYGDTLAAIQGLLKKHEAFETDFTVHKDRVNDVCTNGQDLVKKNNHHEENISSKMKGLNGKVSDLEKAASQRKAKLDENSAFLQFNWKADVVESWIGEKENSLKTDDYGRDLSSVQTLLTKQETFDAGLQAFQQEGIANITALKDQLLAAKHVQSKAIEARHAALMKRWSQLLANSATRKKKLLEAQSHFRKVEDLFLTFAKKASAFNSWFENAEEDLTDPVRCNSLEEIKALREAHDAFRSSLSSAQADFNQLAELDRQIKSFRVASNPYTWFTMEALEETWRNLQKIIKERELELQKEQRRQEENDKLRQEFAQHANAFHQWIQETRTYLLDGIAYRRVIRVYQYEVGDDLSGRSCMVEESGTLESQLEATKRKHQEIRAMRSQLKKIEDLGAAMEEALILDNKYTEHSTVGLAQQWDQLDQLGMRMQHNLEQQIQARNTTGVTEEALKEFSMMFKHFDKDKSGRLNHQEFKSCLRSLGYDLPMVEEGEPDPEFEAILDTVDPNRDGHVSLQEYMAFMISRETENVKSSEEIESAFRALSSEGKPYVTKEELYQNLTREQADYCVSHMKPYVDGKGRELPTAFDYVEFTRSLFVN

Target ORF information:

RefSeq Version	XM_027966356.1
Organism	Ovis aries(sheep)
Definition	Ovis aries spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027966356.1

ORF Insert Sequence:

ATGGACCCAA GCGGGGTCAA AGTGCTGGAG ACAGCCGAGG ACATCCAGGA GAGGCGGCAG 
CAGGTCCTGG ACAGATACCA CCGCTTCAAG GAGCTCTCGA CCCTGAGGCG CCAGAAGCTT 
GAAGACTCCT ACCGATTCCA GTTTTTCCAA AGAGATGCTG AGGAGCTGGA GAAATGGATT 
CAGGAGAAGC TTCAGATTGC CTCGGATGAG AATTACAAAG ACCCAACCAA CCTGCAGGGC 
AAGCTGCAGA AGCATCAAGC CTTCGAAGCC GAGGTGCAGG CCAACTCGGG TGCCATCGTG 
AAGCTTGATG AGACCGGTAA CCTGATGATC TCAGAAGGGC ACTTCGCGTC CGAAACCATC 
CGGACTCGTC TGATGGAGCT GCACCGCCAG TGGGAATTGC TTCTGGAGAA GATGCGGGAG 
AAAGGCATCA AGCTGCTGCA GGCCCAGAAG CTGGTGCAGT ACCTGCGGGA GTGTGAGGAC 
GTGATGGACT GGATCAATGA CAAGGAAGCC ATTGTGACAT CTGAGGAGCT GGGCCAGGAC 
CTGGAGCACG TGGAGGTGTT ACAGAAGAAA TTCGAAGAGT TTCAGACAGA CATGGCTGCC 
CACGAAGAGA GAGTCAATGA AGTGAACCAG TTTGCTGCCA AGCTCATTCA GGAGCAGCAC 
CCTGAGGAGG AGCTGATCAA GACAAAGGAG GACGAGGTGA ATGCAGCCTG GCAGCGGCTG 
AAGGGCCTGG CCCTCCAGCG GCAGGGGAAG CTGTTCGGGG CAGCTGAGGT CCAACGCTTT 
AACAGGGATG TGGACGAGAC CATTAGCTGG ATTAAGGAGA AGGAACAGTT AATGGCCTCT 
GATGACTTTG GCCGGGACCT GGCCAGTGTC CAGGCTCTAC TGCGGAAACA CGAGGGTTTG 
GAGAGAGATC TTGCTGCCTT GGAGGACAAG GTCAAAGCCC TGTGTGCCGA GGCTGACCGC 
CTGCAGCAGT CCCACCCTCT GAGTGCCACC CAGATCCAAG TGAAGCGAGA GGAGCTGATC 
ACCAACTGGG AGCAGATCCG CACCCTGGCC GCGGAGAGAC ACGCACGTCT CAACGATTCC 
TACAGGCTTC AGCGCTTCCT TGCTGACTTC CGAGACCTCA CCAGCTGGGT CACTGAGATG 
AAAGCCCTCA TCAATGCAGA TGAACTTGCC AATGATGTGG CCGGGGCCGA AGCCCTGCTG 
GATAGGCATC AGGAGCACAA GGGTGAAATT GACGCTCATG AAGATAGCTT TAAATCTGCA 
GATGAGTCTG GACAGGCCCT GCTTGCAGCT GGTCACTATG CCTCAGATGA AGTGAGGGAG 
AAGCTGACCA TCCTGTCCGA GGAGCGGGCC GCGCTACTAG AGCTCTGGGA GCTGCGACGG 
CAGCAATATG AGCAGTGCAT GGACCTGCAG CTCTTCTACC GCGACACGGA GCAGGTGGAC 
AACTGGATGA GCAAACAGGA GGCGTTCCTG TTGAACGAAG ACCTGGGTGA TTCCTTGGAT 
AGTGTGGAAG CGCTTCTCAA GAAGCACGAA GACTTTGAGA AGTCCCTCAG CGCCCAGGAG 
GAGAAGATCA CAGCCTTAGA TGAGTTTGCC ACCAAGCTGA TTCAGAACAA CCACTACGCG 
ATGGAAGATG TGGCCACCCG GCGAGACGCA CTGCTGAGCC GCCGCAACGC CCTCCATGAG 
AGAGCCATGT ACCGCCGTGC CCAGCTTGCA GATTCCTTCC ACTTGCAGCA GTTTTTCCGT 
GACTCTGATG AGCTCAAGAG TTGGGTCAAC GAGAAGATGA AAACAGCCAC AGATGAAGCT 
TATAAAGACC CATCCAACCT ACAAGGGAAA GTGCAGAAGC ACCAGGCCTT TGAGGCCGAG 
CTCTCAGCAA ACCAGAGCCG CATCGACGCC TTGGAGAAAG CTGGGCAGAA GCTGATCGAC 
GTTGACCACT ATGCCAAGGA TGAGGTGGCG ACTCGGATGA ATGAAGTTAT CAGTCTGTGG 
AAGAAGCTGC TGGAGGCCAC GGAGCTGAAA GGCATAAAGC TCCGTGAGGC TAACCAGCAG 
CAGCAGTTTA ACCGCAACGT TGAGGACATC GAGCTGTGGC TGTACGAAGT GGAAGGTCAT 
CTGGCCTCGG ATGACTACGG CAAAGACCTC ACCAACGTCC AGAACCTCCA GAAAAAGCAT 
GCCCTGCTGG AGGCTGACGT GGCCGCTCAC CAGGATCGCA TCGATGGCAT CACTATCCAA 
GCCCGCCAGT TCCAGGATGC GGGCCACTTT GATGCTGAAA ACATCAAGAA GAAGCAGGAG 
GCCCTGGTAG CTCGCTACGA GGCACTCAAG GAGCCCATGG TTGCCCGGAA GCAGAAGCTG 
GCCGATTCCC TGCGGTTGCA GCAGCTCTTC CGTGACGTTG AGGATGAGGA GACGTGGATT 
CGGGAGAAAG AACCTATTGC CGCCTCCACC AACAGAGGCA AAGATTTAAT TGGAGTCCAG 
AACCTGCTAA AGAAACACCA AGCGCTACAA GCAGAAATTG CTGGCCACGA GCCCCGCATC 
AAAGCAGTCA CACAGAAGGG GAACGCCATG GTAGAGGAGG GCCATTTTGC TGCCGAGGAC 
GTGAAGGCCA AGCTCCATGA ACTGAACCAG AAGTGGGAGT CGCTGAAGTC CAAGGCCTCC 
CAGCGACGGC AGGACCTGGA GGACTCGCTG CAGGCCCAGC AGTACTTCGC TGATGCCAAC 
GAGGCCGAGT CCTGGATGCG GGAGAAGGAG CCCATCGTGG GCAGCACCGA CTATGGCAAA 
GATGAGGACT CTGCCGAGGC TCTGCTAAAG AAGCACGAGG CATTGATGTC GGATCTCAGC 
GCCTATGGCA GCAGCATCCA GGCCCTGCGA GAGCAGGCCC AGTCGTGCCG GCAACAAGTG 
GCCCCCATGG ATGACGAGAC TGGGAAGGAG CTGGTCTTGG CGCTCTATGA CTATCAGGAG 
AAGAGTCCCC GAGAGGTCAC CATGAAGAAA GGAGACATTC TCACCTTACT CAACAGCACC 
AACAAGGACT GGTGGAAAGT GGAAGTGAAC GACCGGCAGG GCTTCGTGCC GGCCGCCTAC 
GTGAAGAAGC TGGACCCAGC GCAGTCGGCC TCCCGGGAGA ACCTCCTGGA AGAGCAGGGC 
AGCATCGCGC TGCGGCAGGA GCAGATTGAC AACCAGACGC GCGTAACTAA GGAGGCCGGC 
AGCGTGTCTC TGCGTATGAA ACAGGTGGAA GAACTGTACC ATTCTCTGCT GGAACTGGGA 
GAGAAGCGTA AAGGCATGTT GGAGAAGAGC TGTAAGAAGT TCATGCTGTT CCGTGAAGCG 
AACGAGCTGC AGCAGTGGAT CAACGAGAAG GAAGCGGCCC TGACAAGCGA GGAGGTCGGC 
GCGGACTTGG AGCAGGTCGA AGTGCTGCAG AAGAAGTTTG ACGACTTCCA GAAGGATCTC 
AAGGCCAATG AGTCACGGCT GAAGGACATT AACAAGGTGG CTGAAGACCT GGAGTCGGAA 
GGCCTGATGG CGGAGGAGGT GCAGGCCGTG CAGCAGCAGG AGGTGTATGG CGCGATGCCC 
AGGGATGAAT CTGATTCCAA GACAGCCTCC CCGTGGAAGT CTGCTCGCCT GATGGTTCAC 
ACCGTGGCCA CCTTTAATTC CATCAAGGAG CTCAATGAGC GCTGGCGGTC CCTGCAGCAG 
CTGGCTGAGG AGCGGAGCCA ACTCTTGGGC AGCGCCCACG AAGTGCAGCG GTTCCACAGA 
GATGCTGACG AAACCAAAGA GTGGATCGAG GAGAAGAACC AGGCTCTAAA CACAGACAAC 
TATGGCCACG ACCTGGCCAG CGTCCAGGCC CTGCAGCGCA AGCATGAGGG CTTCGAGAGG 
GACCTCGCGG CCCTTGGCGA CAAGGTGAAC TCGCTCGGGG AGACAGCCGA GCGCCTGATC 
CAGTCCCACC CCGAGTCGGC AGAGGACCTG CAGGAGAAGT GCACTGAGCT GAACCAGGCA 
TGGAGCAGCC TGGGCAAGCG TGCCGACCAG CGCAAGGCCA AGCTGGGCGA CTCCCACGAC 
CTGCAGCGCT TCCTCAGCGA CTTCCGGGAC CTCATGTCTT GGATCAATGG AATCCGAGGT 
CTGGTGTCCT CAGACGAGCT AGCCAAGGAT GTCACTGGAG CTGAGGCCCT GCTGGAGCGA 
CACCAGGAAC ACCGGACAGA AATTGATGCC AGAGCTGGCA CTTTCCAGGC ATTCGAGCAG 
TTTGGGCAGC AGCTGTTGGC TCATGGGCAC TACGCCAGCC CCGAGATCAA GGAGAAGCTC 
GATGTTCTAG ACCAGGAGCG CGCAGACCTC GAGAAGGCCT GGGTCCAGCG CAGGATGATG 
CTGGATCAGT GCCTTGAATT GCAGCTGTTC CATCGGGATT GCGAGCAGGC CGAGAACTGG 
ATGGCTGCCC GGGAGGCCTT CCTGAACACA GAAGACAAAG GGGACTCGCT AGACAGCGTG 
GAGGCTCTGA TCAAAAAACA CGAGGACTTT GACAAAGCAA TTAATGTCCA GGAGGAGAAG 
ATTGCTGCCC TGCAGTCCTT TGCTGACCAG CTCATTGCTG GCGGTCACTA CGCCAAAGGC 
GACATCTCCA GCCGGCGCAA TGAGGTTTTG GACAGGTGGC GCCGTCTAAA AGCTCAGATG 
ATTGAGAAGA GGTCAAAGCT GGGAGAATCG CAGACCCTCC AGCAGTTCAG CCGGGATGTA 
GACGAGATTG AAGCTTGGAT CAGTGAAAAA TTACAGACTG CAAGTGATGA GTCATATAAG 
GACCCCACCA ACATCCAGCT TTCCAAGCTG CTGAGCAAGC ATCAGAAGCA CCAGGCCTTC 
GAGGCAGAAC TGCACGCCAA TGCGGACCGG ATCCGCGGGG TCATCGACGT GGGCAACTCC 
CTCATTGACC GTGGGGCCTG TGCCGGCAGC GAGGATGCCG TCAAGGCCCG GCTGGCCGCC 
TTAGCTGACC AGTGGCAGTT CCTGGTGCAG AAGTCGGCTG AGAAGAGCCA GAAGCTGAAA 
GAAGCCAACA AACAGCAGAA CTTCAACACT GGCATCAAGG ACTTCGACTT CTGGCTCTCT 
GAGGTGGAAG CCCTGCTGGC CTCTGAGGAT TATGGCAAAG ACCTGGCTTC TGTGAACAAC 
TTGCTAAAAA AACATCAGCT GCTGGAGGCA GATATCTCTG CCCACGAGGA CCGCCTGAAG 
GACCTCAACA GCCAGGCGGA CAGCCTGATG ACCAGCAGCG CCTTCGACAC CTCCCAGGTG 
AAGGATAAGC GGGACACCAT CAACAGCCGT TTCCAGAAGA TCAAGAGCAT GGCCGCCTCC 
CGGCGAGCTA GGCTGAATGA GTCCCACCGC CTGCACCAGT TCTTCCGGGA CATGGACGAT 
GAGGAGTCCT GGATCAAGGA GAAGAAGCTG CTGGTGAGCT CAGAGGACTA CGGCCGCGAC 
CTGACGGGCG TCCAGAACCT GAGGAAGAAG CACAAACGGC TGGAGGCAGA GCTGGCTGCC 
CACGAGCCGG CCATTCAGGG TGTCCTGGAC ACTGGAAAGA AGCTGTCTGA TGACAACACC 
ATCGGGAAGG AGGAGATTCA GCAGCGCCTG GCGCAGTTTG TGGAGCACTG GAAGGAGCTG 
AAGCAGCTGG CAGCTGCTCG GGGCCAGCGG CTAGAAGAAT CTTTGGAATA TCAGCAGTTT 
GTGGCCAACG TGGAGGAGGA GGAAGCCTGG ATCAATGAGA AGATGACCCT GGTGGCCAGT 
GAGGATTACG GAGACACTCT TGCTGCCATC CAGGGCTTGC TGAAGAAACA CGAAGCCTTC 
GAGACGGACT TCACTGTCCA CAAGGACCGG GTGAATGACG TCTGCACTAA CGGACAAGAC 
CTGGTTAAGA AGAACAATCA CCATGAGGAG AACATCTCTT CCAAGATGAA GGGCTTGAAC 
GGGAAAGTGT CAGACTTGGA GAAGGCTGCC TCCCAGAGGA AGGCAAAGCT CGACGAGAAC 
TCGGCCTTCC TCCAGTTCAA CTGGAAGGCC GATGTGGTGG AGTCCTGGAT TGGTGAAAAG 
GAGAACAGCT TGAAGACAGA TGATTATGGT CGAGATCTGT CTTCTGTCCA AACTCTCCTC 
ACCAAACAGG AAACCTTCGA TGCTGGCCTG CAGGCCTTCC AGCAGGAGGG CATCGCCAAC 
ATCACTGCCC TCAAAGATCA GCTGCTGGCC GCCAAGCATG TTCAGTCCAA GGCCATCGAG 
GCCCGCCACG CTGCCCTCAT GAAGAGATGG AGCCAGCTCC TGGCCAATTC GGCCACCCGG 
AAGAAGAAGC TGCTGGAGGC ACAGAGTCAC TTCCGCAAGG TGGAGGATCT CTTCCTGACC 
TTTGCCAAAA AGGCTTCAGC CTTCAACAGC TGGTTTGAAA ATGCAGAGGA GGACTTAACA 
GACCCAGTGC GCTGCAACTC CCTGGAAGAG ATCAAGGCCT TGAGAGAGGC CCACGATGCC 
TTCCGCTCCT CGCTGAGCTC AGCCCAGGCC GACTTCAACC AGCTGGCTGA GCTGGACCGC 
CAGATCAAGA GCTTCCGCGT GGCCTCCAAC CCCTACACCT GGTTCACCAT GGAGGCCCTG 
GAGGAGACCT GGAGGAACCT TCAGAAGATC ATCAAGGAAA GGGAGCTGGA GCTGCAGAAG 
GAGCAGCGGC GGCAGGAGGA GAACGACAAG CTGCGCCAGG AGTTCGCCCA GCATGCCAAC 
GCCTTCCACC AGTGGATCCA GGAGACCAGA ACGTATCTCC TCGATGGCAT AGCGTATCGT 
CGGGTCATTC GTGTCTATCA GTATGAAGTT GGGGATGATC TGTCTGGAAG GTCCTGCATG 
GTGGAAGAAT CTGGGACCCT TGAATCGCAG CTTGAAGCTA CCAAACGCAA ACACCAGGAG 
ATTCGAGCCA TGAGAAGTCA GCTCAAGAAG ATTGAAGACC TGGGGGCGGC CATGGAGGAA 
GCGCTGATCC TGGACAACAA GTACACGGAG CACAGCACCG TGGGCCTGGC CCAGCAGTGG 
GACCAGCTCG ACCAGCTGGG CATGCGGATG CAGCACAACC TGGAGCAGCA GATCCAGGCC 
AGGAACACGA CAGGAGTGAC CGAGGAGGCC CTCAAAGAAT TCAGCATGAT GTTCAAACAC 
TTCGACAAGG ACAAGTCCGG CCGGCTGAAT CACCAGGAGT TCAAATCCTG TCTGCGCTCC 
CTGGGCTATG ACCTGCCCAT GGTGGAGGAA GGGGAGCCCG ACCCTGAGTT CGAGGCCATC 
TTGGATACTG TGGATCCTAA CAGGGACGGC CACGTCTCCC TGCAAGAGTA CATGGCCTTT 
ATGATCAGCC GTGAGACCGA GAATGTCAAG TCCAGCGAGG AGATTGAGAG CGCCTTCCGG 
GCCCTGAGCT CCGAAGGGAA GCCTTATGTG ACCAAGGAGG AGCTGTACCA GAACCTGACT 
CGGGAACAAG CCGACTACTG CGTCTCCCAC ATGAAGCCCT ACGTGGACGG CAAGGGCCGC 
GAACTCCCCA CTGCTTTCGA CTACGTTGAG TTCACCCGCT CGCTCTTCGT GAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTAN1 ( XM_027966357.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_027822158.1 Ovis aries spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X4, mRNA.

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CloneID	OSm76651
Clone ID Related Accession (Same CDS sequence)	XM_027966357.1
Accession Version	XM_027966357.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7485bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-05
Organism	Ovis aries(sheep)
Product	spectrin alpha chain, non-erythrocytic 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040254.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ovis aries Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTCAT CGTTTCGTAA ACGCAGAGTC CAGAAAATGG ACCCAAGCGG GGTCAAAGTG 
CTGGAGACAG CCGAGGACAT CCAGGAGAGG CGGCAGCAGG TCCTGGACAG ATACCACCGC 
TTCAAGGAGC TCTCGACCCT GAGGCGCCAG AAGCTTGAAG ACTCCTACCG ATTCCAGTTT 
TTCCAAAGAG ATGCTGAGGA GCTGGAGAAA TGGATTCAGG AGAAGCTTCA GATTGCCTCG 
GATGAGAATT ACAAAGACCC AACCAACCTG CAGGGCAAGC TGCAGAAGCA TCAAGCCTTC 
GAAGCCGAGG TGCAGGCCAA CTCGGGTGCC ATCGTGAAGC TTGATGAGAC CGGTAACCTG 
ATGATCTCAG AAGGGCACTT CGCGTCCGAA ACCATCCGGA CTCGTCTGAT GGAGCTGCAC 
CGCCAGTGGG AATTGCTTCT GGAGAAGATG CGGGAGAAAG GCATCAAGCT GCTGCAGGCC 
CAGAAGCTGG TGCAGTACCT GCGGGAGTGT GAGGACGTGA TGGACTGGAT CAATGACAAG 
GAAGCCATTG TGACATCTGA GGAGCTGGGC CAGGACCTGG AGCACGTGGA GGTGTTACAG 
AAGAAATTCG AAGAGTTTCA GACAGACATG GCTGCCCACG AAGAGAGAGT CAATGAAGTG 
AACCAGTTTG CTGCCAAGCT CATTCAGGAG CAGCACCCTG AGGAGGAGCT GATCAAGACA 
AAGGAGGACG AGGTGAATGC AGCCTGGCAG CGGCTGAAGG GCCTGGCCCT CCAGCGGCAG 
GGGAAGCTGT TCGGGGCAGC TGAGGTCCAA CGCTTTAACA GGGATGTGGA CGAGACCATT 
AGCTGGATTA AGGAGAAGGA ACAGTTAATG GCCTCTGATG ACTTTGGCCG GGACCTGGCC 
AGTGTCCAGG CTCTACTGCG GAAACACGAG GGTTTGGAGA GAGATCTTGC TGCCTTGGAG 
GACAAGGTCA AAGCCCTGTG TGCCGAGGCT GACCGCCTGC AGCAGTCCCA CCCTCTGAGT 
GCCACCCAGA TCCAAGTGAA GCGAGAGGAG CTGATCACCA ACTGGGAGCA GATCCGCACC 
CTGGCCGCGG AGAGACACGC ACGTCTCAAC GATTCCTACA GGCTTCAGCG CTTCCTTGCT 
GACTTCCGAG ACCTCACCAG CTGGGTCACT GAGATGAAAG CCCTCATCAA TGCAGATGAA 
CTTGCCAATG ATGTGGCCGG GGCCGAAGCC CTGCTGGATA GGCATCAGGA GCACAAGGGT 
GAAATTGACG CTCATGAAGA TAGCTTTAAA TCTGCAGATG AGTCTGGACA GGCCCTGCTT 
GCAGCTGGTC ACTATGCCTC AGATGAAGTG AGGGAGAAGC TGACCATCCT GTCCGAGGAG 
CGGGCCGCGC TACTAGAGCT CTGGGAGCTG CGACGGCAGC AATATGAGCA GTGCATGGAC 
CTGCAGCTCT TCTACCGCGA CACGGAGCAG GTGGACAACT GGATGAGCAA ACAGGAGGCG 
TTCCTGTTGA ACGAAGACCT GGGTGATTCC TTGGATAGTG TGGAAGCGCT TCTCAAGAAG 
CACGAAGACT TTGAGAAGTC CCTCAGCGCC CAGGAGGAGA AGATCACAGC CTTAGATGAG 
TTTGCCACCA AGCTGATTCA GAACAACCAC TACGCGATGG AAGATGTGGC CACCCGGCGA 
GACGCACTGC TGAGCCGCCG CAACGCCCTC CATGAGAGAG CCATGTACCG CCGTGCCCAG 
CTTGCAGATT CCTTCCACTT GCAGCAGTTT TTCCGTGACT CTGATGAGCT CAAGAGTTGG 
GTCAACGAGA AGATGAAAAC AGCCACAGAT GAAGCTTATA AAGACCCATC CAACCTACAA 
GGGAAAGTGC AGAAGCACCA GGCCTTTGAG GCCGAGCTCT CAGCAAACCA GAGCCGCATC 
GACGCCTTGG AGAAAGCTGG GCAGAAGCTG ATCGACGTTG ACCACTATGC CAAGGATGAG 
GTGGCGACTC GGATGAATGA AGTTATCAGT CTGTGGAAGA AGCTGCTGGA GGCCACGGAG 
CTGAAAGGCA TAAAGCTCCG TGAGGCTAAC CAGCAGCAGC AGTTTAACCG CAACGTTGAG 
GACATCGAGC TGTGGCTGTA CGAAGTGGAA GGTCATCTGG CCTCGGATGA CTACGGCAAA 
GACCTCACCA ACGTCCAGAA CCTCCAGAAA AAGCATGCCC TGCTGGAGGC TGACGTGGCC 
GCTCACCAGG ATCGCATCGA TGGCATCACT ATCCAAGCCC GCCAGTTCCA GGATGCGGGC 
CACTTTGATG CTGAAAACAT CAAGAAGAAG CAGGAGGCCC TGGTAGCTCG CTACGAGGCA 
CTCAAGGAGC CCATGGTTGC CCGGAAGCAG AAGCTGGCCG ATTCCCTGCG GTTGCAGCAG 
CTCTTCCGTG ACGTTGAGGA TGAGGAGACG TGGATTCGGG AGAAAGAACC TATTGCCGCC 
TCCACCAACA GAGGCAAAGA TTTAATTGGA GTCCAGAACC TGCTAAAGAA ACACCAAGCG 
CTACAAGCAG AAATTGCTGG CCACGAGCCC CGCATCAAAG CAGTCACACA GAAGGGGAAC 
GCCATGGTAG AGGAGGGCCA TTTTGCTGCC GAGGACGTGA AGGCCAAGCT CCATGAACTG 
AACCAGAAGT GGGAGTCGCT GAAGTCCAAG GCCTCCCAGC GACGGCAGGA CCTGGAGGAC 
TCGCTGCAGG CCCAGCAGTA CTTCGCTGAT GCCAACGAGG CCGAGTCCTG GATGCGGGAG 
AAGGAGCCCA TCGTGGGCAG CACCGACTAT GGCAAAGATG AGGACTCTGC CGAGGCTCTG 
CTAAAGAAGC ACGAGGCATT GATGTCGGAT CTCAGCGCCT ATGGCAGCAG CATCCAGGCC 
CTGCGAGAGC AGGCCCAGTC GTGCCGGCAA CAAGTGGCCC CCATGGATGA CGAGACTGGG 
AAGGAGCTGG TCTTGGCGCT CTATGACTAT CAGGAGAAGA GTCCCCGAGA GGTCACCATG 
AAGAAAGGAG ACATTCTCAC CTTACTCAAC AGCACCAACA AGGACTGGTG GAAAGTGGAA 
GTGAACGACC GGCAGGGCTT CGTGCCGGCC GCCTACGTGA AGAAGCTGGA CCCAGCGCAG 
TCGGCCTCCC GGGAGAACCT CCTGGAAGAG CAGGGCAGCA TCGCGCTGCG GCAGGAGCAG 
ATTGACAACC AGACGCGCGT AACTAAGGAG GCCGGCAGCG TGTCTCTGCG TATGAAACAG 
GTGGAAGAAC TGTACCATTC TCTGCTGGAA CTGGGAGAGA AGCGTAAAGG CATGTTGGAG 
AAGAGCTGTA AGAAGTTCAT GCTGTTCCGT GAAGCGAACG AGCTGCAGCA GTGGATCAAC 
GAGAAGGAAG CGGCCCTGAC AAGCGAGGAG GTCGGCGCGG ACTTGGAGCA GGTCGAAGTG 
CTGCAGAAGA AGTTTGACGA CTTCCAGAAG GATCTCAAGG CCAATGAGTC ACGGCTGAAG 
GACATTAACA AGGTGGCTGA AGACCTGGAG TCGGAAGGCC TGATGGCGGA GGAGGTGCAG 
GCCGTGCAGC AGCAGGAGGT GTATGGCGCG ATGCCCAGGG ATGAATCTGA TTCCAAGACA 
GCCTCCCCGT GGAAGGAGCT CAATGAGCGC TGGCGGTCCC TGCAGCAGCT GGCTGAGGAG 
CGGAGCCAAC TCTTGGGCAG CGCCCACGAA GTGCAGCGGT TCCACAGAGA TGCTGACGAA 
ACCAAAGAGT GGATCGAGGA GAAGAACCAG GCTCTAAACA CAGACAACTA TGGCCACGAC 
CTGGCCAGCG TCCAGGCCCT GCAGCGCAAG CATGAGGGCT TCGAGAGGGA CCTCGCGGCC 
CTTGGCGACA AGGTGAACTC GCTCGGGGAG ACAGCCGAGC GCCTGATCCA GTCCCACCCC 
GAGTCGGCAG AGGACCTGCA GGAGAAGTGC ACTGAGCTGA ACCAGGCATG GAGCAGCCTG 
GGCAAGCGTG CCGACCAGCG CAAGGCCAAG CTGGGCGACT CCCACGACCT GCAGCGCTTC 
CTCAGCGACT TCCGGGACCT CATGTCTTGG ATCAATGGAA TCCGAGGTCT GGTGTCCTCA 
GACGAGCTAG CCAAGGATGT CACTGGAGCT GAGGCCCTGC TGGAGCGACA CCAGGAACAC 
CGGACAGAAA TTGATGCCAG AGCTGGCACT TTCCAGGCAT TCGAGCAGTT TGGGCAGCAG 
CTGTTGGCTC ATGGGCACTA CGCCAGCCCC GAGATCAAGG AGAAGCTCGA TGTTCTAGAC 
CAGGAGCGCG CAGACCTCGA GAAGGCCTGG GTCCAGCGCA GGATGATGCT GGATCAGTGC 
CTTGAATTGC AGCTGTTCCA TCGGGATTGC GAGCAGGCCG AGAACTGGAT GGCTGCCCGG 
GAGGCCTTCC TGAACACAGA AGACAAAGGG GACTCGCTAG ACAGCGTGGA GGCTCTGATC 
AAAAAACACG AGGACTTTGA CAAAGCAATT AATGTCCAGG AGGAGAAGAT TGCTGCCCTG 
CAGTCCTTTG CTGACCAGCT CATTGCTGGC GGTCACTACG CCAAAGGCGA CATCTCCAGC 
CGGCGCAATG AGGTTTTGGA CAGGTGGCGC CGTCTAAAAG CTCAGATGAT TGAGAAGAGG 
TCAAAGCTGG GAGAATCGCA GACCCTCCAG CAGTTCAGCC GGGATGTAGA CGAGATTGAA 
GCTTGGATCA GTGAAAAATT ACAGACTGCA AGTGATGAGT CATATAAGGA CCCCACCAAC 
ATCCAGCTTT CCAAGCTGCT GAGCAAGCAT CAGAAGCACC AGGCCTTCGA GGCAGAACTG 
CACGCCAATG CGGACCGGAT CCGCGGGGTC ATCGACGTGG GCAACTCCCT CATTGACCGT 
GGGGCCTGTG CCGGCAGCGA GGATGCCGTC AAGGCCCGGC TGGCCGCCTT AGCTGACCAG 
TGGCAGTTCC TGGTGCAGAA GTCGGCTGAG AAGAGCCAGA AGCTGAAAGA AGCCAACAAA 
CAGCAGAACT TCAACACTGG CATCAAGGAC TTCGACTTCT GGCTCTCTGA GGTGGAAGCC 
CTGCTGGCCT CTGAGGATTA TGGCAAAGAC CTGGCTTCTG TGAACAACTT GCTAAAAAAA 
CATCAGCTGC TGGAGGCAGA TATCTCTGCC CACGAGGACC GCCTGAAGGA CCTCAACAGC 
CAGGCGGACA GCCTGATGAC CAGCAGCGCC TTCGACACCT CCCAGGTGAA GGATAAGCGG 
GACACCATCA ACAGCCGTTT CCAGAAGATC AAGAGCATGG CCGCCTCCCG GCGAGCTAGG 
CTGAATGAGT CCCACCGCCT GCACCAGTTC TTCCGGGACA TGGACGATGA GGAGTCCTGG 
ATCAAGGAGA AGAAGCTGCT GGTGAGCTCA GAGGACTACG GCCGCGACCT GACGGGCGTC 
CAGAACCTGA GGAAGAAGCA CAAACGGCTG GAGGCAGAGC TGGCTGCCCA CGAGCCGGCC 
ATTCAGGGTG TCCTGGACAC TGGAAAGAAG CTGTCTGATG ACAACACCAT CGGGAAGGAG 
GAGATTCAGC AGCGCCTGGC GCAGTTTGTG GAGCACTGGA AGGAGCTGAA GCAGCTGGCA 
GCTGCTCGGG GCCAGCGGCT AGAAGAATCT TTGGAATATC AGCAGTTTGT GGCCAACGTG 
GAGGAGGAGG AAGCCTGGAT CAATGAGAAG ATGACCCTGG TGGCCAGTGA GGATTACGGA 
GACACTCTTG CTGCCATCCA GGGCTTGCTG AAGAAACACG AAGCCTTCGA GACGGACTTC 
ACTGTCCACA AGGACCGGGT GAATGACGTC TGCACTAACG GACAAGACCT GGTTAAGAAG 
AACAATCACC ATGAGGAGAA CATCTCTTCC AAGATGAAGG GCTTGAACGG GAAAGTGTCA 
GACTTGGAGA AGGCTGCCTC CCAGAGGAAG GCAAAGCTCG ACGAGAACTC GGCCTTCCTC 
CAGTTCAACT GGAAGGCCGA TGTGGTGGAG TCCTGGATTG GTGAAAAGGA GAACAGCTTG 
AAGACAGATG ATTATGGTCG AGATCTGTCT TCTGTCCAAA CTCTCCTCAC CAAACAGGAA 
ACCTTCGATG CTGGCCTGCA GGCCTTCCAG CAGGAGGGCA TCGCCAACAT CACTGCCCTC 
AAAGATCAGC TGCTGGCCGC CAAGCATGTT CAGTCCAAGG CCATCGAGGC CCGCCACGCT 
GCCCTCATGA AGAGATGGAG CCAGCTCCTG GCCAATTCGG CCACCCGGAA GAAGAAGCTG 
CTGGAGGCAC AGAGTCACTT CCGCAAGGTG GAGGATCTCT TCCTGACCTT TGCCAAAAAG 
GCTTCAGCCT TCAACAGCTG GTTTGAAAAT GCAGAGGAGG ACTTAACAGA CCCAGTGCGC 
TGCAACTCCC TGGAAGAGAT CAAGGCCTTG AGAGAGGCCC ACGATGCCTT CCGCTCCTCG 
CTGAGCTCAG CCCAGGCCGA CTTCAACCAG CTGGCTGAGC TGGACCGCCA GATCAAGAGC 
TTCCGCGTGG CCTCCAACCC CTACACCTGG TTCACCATGG AGGCCCTGGA GGAGACCTGG 
AGGAACCTTC AGAAGATCAT CAAGGAAAGG GAGCTGGAGC TGCAGAAGGA GCAGCGGCGG 
CAGGAGGAGA ACGACAAGCT GCGCCAGGAG TTCGCCCAGC ATGCCAACGC CTTCCACCAG 
TGGATCCAGG AGACCAGAAC GTATCTCCTC GATGGCATAG CGTATCGTCG GGTCATTCGT 
GTCTATCAGT ATGAAGTTGG GGATGATCTG TCTGGAAGGT CCTGCATGGT GGAAGAATCT 
GGGACCCTTG AATCGCAGCT TGAAGCTACC AAACGCAAAC ACCAGGAGAT TCGAGCCATG 
AGAAGTCAGC TCAAGAAGAT TGAAGACCTG GGGGCGGCCA TGGAGGAAGC GCTGATCCTG 
GACAACAAGT ACACGGAGCA CAGCACCGTG GGCCTGGCCC AGCAGTGGGA CCAGCTCGAC 
CAGCTGGGCA TGCGGATGCA GCACAACCTG GAGCAGCAGA TCCAGGCCAG GAACACGACA 
GGAGTGACCG AGGAGGCCCT CAAAGAATTC AGCATGATGT TCAAACACTT CGACAAGGAC 
AAGTCCGGCC GGCTGAATCA CCAGGAGTTC AAATCCTGTC TGCGCTCCCT GGGCTATGAC 
CTGCCCATGG TGGAGGAAGG GGAGCCCGAC CCTGAGTTCG AGGCCATCTT GGATACTGTG 
GATCCTAACA GGGACGGCCA CGTCTCCCTG CAAGAGTACA TGGCCTTTAT GATCAGCCGT 
GAGACCGAGA ATGTCAAGTC CAGCGAGGAG ATTGAGAGCG CCTTCCGGGC CCTGAGCTCC 
GAAGGGAAGC CTTATGTGAC CAAGGAGGAG CTGTACCAGA ACCTGACTCG GGAACAAGCC 
GACTACTGCG TCTCCCACAT GAAGCCCTAC GTGGACGGCA AGGGCCGCGA ACTCCCCACT 
GCTTTCGACT ACGTTGAGTT CACCCGCTCG CTCTTCGTGA ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_027822158.1
CDS	72..7556
Translation	MLSSFRKRRVQKMDPSGVKVLETAEDIQERRQQVLDRYHRFKELSTLRRQKLEDSYRFQFFQRDAEELEKWIQEKLQIASDENYKDPTNLQGKLQKHQAFEAEVQANSGAIVKLDETGNLMISEGHFASETIRTRLMELHRQWELLLEKMREKGIKLLQAQKLVQYLRECEDVMDWINDKEAIVTSEELGQDLEHVEVLQKKFEEFQTDMAAHEERVNEVNQFAAKLIQEQHPEEELIKTKEDEVNAAWQRLKGLALQRQGKLFGAAEVQRFNRDVDETISWIKEKEQLMASDDFGRDLASVQALLRKHEGLERDLAALEDKVKALCAEADRLQQSHPLSATQIQVKREELITNWEQIRTLAAERHARLNDSYRLQRFLADFRDLTSWVTEMKALINADELANDVAGAEALLDRHQEHKGEIDAHEDSFKSADESGQALLAAGHYASDEVREKLTILSEERAALLELWELRRQQYEQCMDLQLFYRDTEQVDNWMSKQEAFLLNEDLGDSLDSVEALLKKHEDFEKSLSAQEEKITALDEFATKLIQNNHYAMEDVATRRDALLSRRNALHERAMYRRAQLADSFHLQQFFRDSDELKSWVNEKMKTATDEAYKDPSNLQGKVQKHQAFEAELSANQSRIDALEKAGQKLIDVDHYAKDEVATRMNEVISLWKKLLEATELKGIKLREANQQQQFNRNVEDIELWLYEVEGHLASDDYGKDLTNVQNLQKKHALLEADVAAHQDRIDGITIQARQFQDAGHFDAENIKKKQEALVARYEALKEPMVARKQKLADSLRLQQLFRDVEDEETWIREKEPIAASTNRGKDLIGVQNLLKKHQALQAEIAGHEPRIKAVTQKGNAMVEEGHFAAEDVKAKLHELNQKWESLKSKASQRRQDLEDSLQAQQYFADANEAESWMREKEPIVGSTDYGKDEDSAEALLKKHEALMSDLSAYGSSIQALREQAQSCRQQVAPMDDETGKELVLALYDYQEKSPREVTMKKGDILTLLNSTNKDWWKVEVNDRQGFVPAAYVKKLDPAQSASRENLLEEQGSIALRQEQIDNQTRVTKEAGSVSLRMKQVEELYHSLLELGEKRKGMLEKSCKKFMLFREANELQQWINEKEAALTSEEVGADLEQVEVLQKKFDDFQKDLKANESRLKDINKVAEDLESEGLMAEEVQAVQQQEVYGAMPRDESDSKTASPWKELNERWRSLQQLAEERSQLLGSAHEVQRFHRDADETKEWIEEKNQALNTDNYGHDLASVQALQRKHEGFERDLAALGDKVNSLGETAERLIQSHPESAEDLQEKCTELNQAWSSLGKRADQRKAKLGDSHDLQRFLSDFRDLMSWINGIRGLVSSDELAKDVTGAEALLERHQEHRTEIDARAGTFQAFEQFGQQLLAHGHYASPEIKEKLDVLDQERADLEKAWVQRRMMLDQCLELQLFHRDCEQAENWMAAREAFLNTEDKGDSLDSVEALIKKHEDFDKAINVQEEKIAALQSFADQLIAGGHYAKGDISSRRNEVLDRWRRLKAQMIEKRSKLGESQTLQQFSRDVDEIEAWISEKLQTASDESYKDPTNIQLSKLLSKHQKHQAFEAELHANADRIRGVIDVGNSLIDRGACAGSEDAVKARLAALADQWQFLVQKSAEKSQKLKEANKQQNFNTGIKDFDFWLSEVEALLASEDYGKDLASVNNLLKKHQLLEADISAHEDRLKDLNSQADSLMTSSAFDTSQVKDKRDTINSRFQKIKSMAASRRARLNESHRLHQFFRDMDDEESWIKEKKLLVSSEDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQFVEHWKELKQLAAARGQRLEESLEYQQFVANVEEEEAWINEKMTLVASEDYGDTLAAIQGLLKKHEAFETDFTVHKDRVNDVCTNGQDLVKKNNHHEENISSKMKGLNGKVSDLEKAASQRKAKLDENSAFLQFNWKADVVESWIGEKENSLKTDDYGRDLSSVQTLLTKQETFDAGLQAFQQEGIANITALKDQLLAAKHVQSKAIEARHAALMKRWSQLLANSATRKKKLLEAQSHFRKVEDLFLTFAKKASAFNSWFENAEEDLTDPVRCNSLEEIKALREAHDAFRSSLSSAQADFNQLAELDRQIKSFRVASNPYTWFTMEALEETWRNLQKIIKERELELQKEQRRQEENDKLRQEFAQHANAFHQWIQETRTYLLDGIAYRRVIRVYQYEVGDDLSGRSCMVEESGTLESQLEATKRKHQEIRAMRSQLKKIEDLGAAMEEALILDNKYTEHSTVGLAQQWDQLDQLGMRMQHNLEQQIQARNTTGVTEEALKEFSMMFKHFDKDKSGRLNHQEFKSCLRSLGYDLPMVEEGEPDPEFEAILDTVDPNRDGHVSLQEYMAFMISRETENVKSSEEIESAFRALSSEGKPYVTKEELYQNLTREQADYCVSHMKPYVDGKGRELPTAFDYVEFTRSLFVN

Target ORF information:

RefSeq Version	XM_027966357.1
Organism	Ovis aries(sheep)
Definition	Ovis aries spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027966357.1

ORF Insert Sequence:

ATGCTGTCAT CGTTTCGTAA ACGCAGAGTC CAGAAAATGG ACCCAAGCGG GGTCAAAGTG 
CTGGAGACAG CCGAGGACAT CCAGGAGAGG CGGCAGCAGG TCCTGGACAG ATACCACCGC 
TTCAAGGAGC TCTCGACCCT GAGGCGCCAG AAGCTTGAAG ACTCCTACCG ATTCCAGTTT 
TTCCAAAGAG ATGCTGAGGA GCTGGAGAAA TGGATTCAGG AGAAGCTTCA GATTGCCTCG 
GATGAGAATT ACAAAGACCC AACCAACCTG CAGGGCAAGC TGCAGAAGCA TCAAGCCTTC 
GAAGCCGAGG TGCAGGCCAA CTCGGGTGCC ATCGTGAAGC TTGATGAGAC CGGTAACCTG 
ATGATCTCAG AAGGGCACTT CGCGTCCGAA ACCATCCGGA CTCGTCTGAT GGAGCTGCAC 
CGCCAGTGGG AATTGCTTCT GGAGAAGATG CGGGAGAAAG GCATCAAGCT GCTGCAGGCC 
CAGAAGCTGG TGCAGTACCT GCGGGAGTGT GAGGACGTGA TGGACTGGAT CAATGACAAG 
GAAGCCATTG TGACATCTGA GGAGCTGGGC CAGGACCTGG AGCACGTGGA GGTGTTACAG 
AAGAAATTCG AAGAGTTTCA GACAGACATG GCTGCCCACG AAGAGAGAGT CAATGAAGTG 
AACCAGTTTG CTGCCAAGCT CATTCAGGAG CAGCACCCTG AGGAGGAGCT GATCAAGACA 
AAGGAGGACG AGGTGAATGC AGCCTGGCAG CGGCTGAAGG GCCTGGCCCT CCAGCGGCAG 
GGGAAGCTGT TCGGGGCAGC TGAGGTCCAA CGCTTTAACA GGGATGTGGA CGAGACCATT 
AGCTGGATTA AGGAGAAGGA ACAGTTAATG GCCTCTGATG ACTTTGGCCG GGACCTGGCC 
AGTGTCCAGG CTCTACTGCG GAAACACGAG GGTTTGGAGA GAGATCTTGC TGCCTTGGAG 
GACAAGGTCA AAGCCCTGTG TGCCGAGGCT GACCGCCTGC AGCAGTCCCA CCCTCTGAGT 
GCCACCCAGA TCCAAGTGAA GCGAGAGGAG CTGATCACCA ACTGGGAGCA GATCCGCACC 
CTGGCCGCGG AGAGACACGC ACGTCTCAAC GATTCCTACA GGCTTCAGCG CTTCCTTGCT 
GACTTCCGAG ACCTCACCAG CTGGGTCACT GAGATGAAAG CCCTCATCAA TGCAGATGAA 
CTTGCCAATG ATGTGGCCGG GGCCGAAGCC CTGCTGGATA GGCATCAGGA GCACAAGGGT 
GAAATTGACG CTCATGAAGA TAGCTTTAAA TCTGCAGATG AGTCTGGACA GGCCCTGCTT 
GCAGCTGGTC ACTATGCCTC AGATGAAGTG AGGGAGAAGC TGACCATCCT GTCCGAGGAG 
CGGGCCGCGC TACTAGAGCT CTGGGAGCTG CGACGGCAGC AATATGAGCA GTGCATGGAC 
CTGCAGCTCT TCTACCGCGA CACGGAGCAG GTGGACAACT GGATGAGCAA ACAGGAGGCG 
TTCCTGTTGA ACGAAGACCT GGGTGATTCC TTGGATAGTG TGGAAGCGCT TCTCAAGAAG 
CACGAAGACT TTGAGAAGTC CCTCAGCGCC CAGGAGGAGA AGATCACAGC CTTAGATGAG 
TTTGCCACCA AGCTGATTCA GAACAACCAC TACGCGATGG AAGATGTGGC CACCCGGCGA 
GACGCACTGC TGAGCCGCCG CAACGCCCTC CATGAGAGAG CCATGTACCG CCGTGCCCAG 
CTTGCAGATT CCTTCCACTT GCAGCAGTTT TTCCGTGACT CTGATGAGCT CAAGAGTTGG 
GTCAACGAGA AGATGAAAAC AGCCACAGAT GAAGCTTATA AAGACCCATC CAACCTACAA 
GGGAAAGTGC AGAAGCACCA GGCCTTTGAG GCCGAGCTCT CAGCAAACCA GAGCCGCATC 
GACGCCTTGG AGAAAGCTGG GCAGAAGCTG ATCGACGTTG ACCACTATGC CAAGGATGAG 
GTGGCGACTC GGATGAATGA AGTTATCAGT CTGTGGAAGA AGCTGCTGGA GGCCACGGAG 
CTGAAAGGCA TAAAGCTCCG TGAGGCTAAC CAGCAGCAGC AGTTTAACCG CAACGTTGAG 
GACATCGAGC TGTGGCTGTA CGAAGTGGAA GGTCATCTGG CCTCGGATGA CTACGGCAAA 
GACCTCACCA ACGTCCAGAA CCTCCAGAAA AAGCATGCCC TGCTGGAGGC TGACGTGGCC 
GCTCACCAGG ATCGCATCGA TGGCATCACT ATCCAAGCCC GCCAGTTCCA GGATGCGGGC 
CACTTTGATG CTGAAAACAT CAAGAAGAAG CAGGAGGCCC TGGTAGCTCG CTACGAGGCA 
CTCAAGGAGC CCATGGTTGC CCGGAAGCAG AAGCTGGCCG ATTCCCTGCG GTTGCAGCAG 
CTCTTCCGTG ACGTTGAGGA TGAGGAGACG TGGATTCGGG AGAAAGAACC TATTGCCGCC 
TCCACCAACA GAGGCAAAGA TTTAATTGGA GTCCAGAACC TGCTAAAGAA ACACCAAGCG 
CTACAAGCAG AAATTGCTGG CCACGAGCCC CGCATCAAAG CAGTCACACA GAAGGGGAAC 
GCCATGGTAG AGGAGGGCCA TTTTGCTGCC GAGGACGTGA AGGCCAAGCT CCATGAACTG 
AACCAGAAGT GGGAGTCGCT GAAGTCCAAG GCCTCCCAGC GACGGCAGGA CCTGGAGGAC 
TCGCTGCAGG CCCAGCAGTA CTTCGCTGAT GCCAACGAGG CCGAGTCCTG GATGCGGGAG 
AAGGAGCCCA TCGTGGGCAG CACCGACTAT GGCAAAGATG AGGACTCTGC CGAGGCTCTG 
CTAAAGAAGC ACGAGGCATT GATGTCGGAT CTCAGCGCCT ATGGCAGCAG CATCCAGGCC 
CTGCGAGAGC AGGCCCAGTC GTGCCGGCAA CAAGTGGCCC CCATGGATGA CGAGACTGGG 
AAGGAGCTGG TCTTGGCGCT CTATGACTAT CAGGAGAAGA GTCCCCGAGA GGTCACCATG 
AAGAAAGGAG ACATTCTCAC CTTACTCAAC AGCACCAACA AGGACTGGTG GAAAGTGGAA 
GTGAACGACC GGCAGGGCTT CGTGCCGGCC GCCTACGTGA AGAAGCTGGA CCCAGCGCAG 
TCGGCCTCCC GGGAGAACCT CCTGGAAGAG CAGGGCAGCA TCGCGCTGCG GCAGGAGCAG 
ATTGACAACC AGACGCGCGT AACTAAGGAG GCCGGCAGCG TGTCTCTGCG TATGAAACAG 
GTGGAAGAAC TGTACCATTC TCTGCTGGAA CTGGGAGAGA AGCGTAAAGG CATGTTGGAG 
AAGAGCTGTA AGAAGTTCAT GCTGTTCCGT GAAGCGAACG AGCTGCAGCA GTGGATCAAC 
GAGAAGGAAG CGGCCCTGAC AAGCGAGGAG GTCGGCGCGG ACTTGGAGCA GGTCGAAGTG 
CTGCAGAAGA AGTTTGACGA CTTCCAGAAG GATCTCAAGG CCAATGAGTC ACGGCTGAAG 
GACATTAACA AGGTGGCTGA AGACCTGGAG TCGGAAGGCC TGATGGCGGA GGAGGTGCAG 
GCCGTGCAGC AGCAGGAGGT GTATGGCGCG ATGCCCAGGG ATGAATCTGA TTCCAAGACA 
GCCTCCCCGT GGAAGGAGCT CAATGAGCGC TGGCGGTCCC TGCAGCAGCT GGCTGAGGAG 
CGGAGCCAAC TCTTGGGCAG CGCCCACGAA GTGCAGCGGT TCCACAGAGA TGCTGACGAA 
ACCAAAGAGT GGATCGAGGA GAAGAACCAG GCTCTAAACA CAGACAACTA TGGCCACGAC 
CTGGCCAGCG TCCAGGCCCT GCAGCGCAAG CATGAGGGCT TCGAGAGGGA CCTCGCGGCC 
CTTGGCGACA AGGTGAACTC GCTCGGGGAG ACAGCCGAGC GCCTGATCCA GTCCCACCCC 
GAGTCGGCAG AGGACCTGCA GGAGAAGTGC ACTGAGCTGA ACCAGGCATG GAGCAGCCTG 
GGCAAGCGTG CCGACCAGCG CAAGGCCAAG CTGGGCGACT CCCACGACCT GCAGCGCTTC 
CTCAGCGACT TCCGGGACCT CATGTCTTGG ATCAATGGAA TCCGAGGTCT GGTGTCCTCA 
GACGAGCTAG CCAAGGATGT CACTGGAGCT GAGGCCCTGC TGGAGCGACA CCAGGAACAC 
CGGACAGAAA TTGATGCCAG AGCTGGCACT TTCCAGGCAT TCGAGCAGTT TGGGCAGCAG 
CTGTTGGCTC ATGGGCACTA CGCCAGCCCC GAGATCAAGG AGAAGCTCGA TGTTCTAGAC 
CAGGAGCGCG CAGACCTCGA GAAGGCCTGG GTCCAGCGCA GGATGATGCT GGATCAGTGC 
CTTGAATTGC AGCTGTTCCA TCGGGATTGC GAGCAGGCCG AGAACTGGAT GGCTGCCCGG 
GAGGCCTTCC TGAACACAGA AGACAAAGGG GACTCGCTAG ACAGCGTGGA GGCTCTGATC 
AAAAAACACG AGGACTTTGA CAAAGCAATT AATGTCCAGG AGGAGAAGAT TGCTGCCCTG 
CAGTCCTTTG CTGACCAGCT CATTGCTGGC GGTCACTACG CCAAAGGCGA CATCTCCAGC 
CGGCGCAATG AGGTTTTGGA CAGGTGGCGC CGTCTAAAAG CTCAGATGAT TGAGAAGAGG 
TCAAAGCTGG GAGAATCGCA GACCCTCCAG CAGTTCAGCC GGGATGTAGA CGAGATTGAA 
GCTTGGATCA GTGAAAAATT ACAGACTGCA AGTGATGAGT CATATAAGGA CCCCACCAAC 
ATCCAGCTTT CCAAGCTGCT GAGCAAGCAT CAGAAGCACC AGGCCTTCGA GGCAGAACTG 
CACGCCAATG CGGACCGGAT CCGCGGGGTC ATCGACGTGG GCAACTCCCT CATTGACCGT 
GGGGCCTGTG CCGGCAGCGA GGATGCCGTC AAGGCCCGGC TGGCCGCCTT AGCTGACCAG 
TGGCAGTTCC TGGTGCAGAA GTCGGCTGAG AAGAGCCAGA AGCTGAAAGA AGCCAACAAA 
CAGCAGAACT TCAACACTGG CATCAAGGAC TTCGACTTCT GGCTCTCTGA GGTGGAAGCC 
CTGCTGGCCT CTGAGGATTA TGGCAAAGAC CTGGCTTCTG TGAACAACTT GCTAAAAAAA 
CATCAGCTGC TGGAGGCAGA TATCTCTGCC CACGAGGACC GCCTGAAGGA CCTCAACAGC 
CAGGCGGACA GCCTGATGAC CAGCAGCGCC TTCGACACCT CCCAGGTGAA GGATAAGCGG 
GACACCATCA ACAGCCGTTT CCAGAAGATC AAGAGCATGG CCGCCTCCCG GCGAGCTAGG 
CTGAATGAGT CCCACCGCCT GCACCAGTTC TTCCGGGACA TGGACGATGA GGAGTCCTGG 
ATCAAGGAGA AGAAGCTGCT GGTGAGCTCA GAGGACTACG GCCGCGACCT GACGGGCGTC 
CAGAACCTGA GGAAGAAGCA CAAACGGCTG GAGGCAGAGC TGGCTGCCCA CGAGCCGGCC 
ATTCAGGGTG TCCTGGACAC TGGAAAGAAG CTGTCTGATG ACAACACCAT CGGGAAGGAG 
GAGATTCAGC AGCGCCTGGC GCAGTTTGTG GAGCACTGGA AGGAGCTGAA GCAGCTGGCA 
GCTGCTCGGG GCCAGCGGCT AGAAGAATCT TTGGAATATC AGCAGTTTGT GGCCAACGTG 
GAGGAGGAGG AAGCCTGGAT CAATGAGAAG ATGACCCTGG TGGCCAGTGA GGATTACGGA 
GACACTCTTG CTGCCATCCA GGGCTTGCTG AAGAAACACG AAGCCTTCGA GACGGACTTC 
ACTGTCCACA AGGACCGGGT GAATGACGTC TGCACTAACG GACAAGACCT GGTTAAGAAG 
AACAATCACC ATGAGGAGAA CATCTCTTCC AAGATGAAGG GCTTGAACGG GAAAGTGTCA 
GACTTGGAGA AGGCTGCCTC CCAGAGGAAG GCAAAGCTCG ACGAGAACTC GGCCTTCCTC 
CAGTTCAACT GGAAGGCCGA TGTGGTGGAG TCCTGGATTG GTGAAAAGGA GAACAGCTTG 
AAGACAGATG ATTATGGTCG AGATCTGTCT TCTGTCCAAA CTCTCCTCAC CAAACAGGAA 
ACCTTCGATG CTGGCCTGCA GGCCTTCCAG CAGGAGGGCA TCGCCAACAT CACTGCCCTC 
AAAGATCAGC TGCTGGCCGC CAAGCATGTT CAGTCCAAGG CCATCGAGGC CCGCCACGCT 
GCCCTCATGA AGAGATGGAG CCAGCTCCTG GCCAATTCGG CCACCCGGAA GAAGAAGCTG 
CTGGAGGCAC AGAGTCACTT CCGCAAGGTG GAGGATCTCT TCCTGACCTT TGCCAAAAAG 
GCTTCAGCCT TCAACAGCTG GTTTGAAAAT GCAGAGGAGG ACTTAACAGA CCCAGTGCGC 
TGCAACTCCC TGGAAGAGAT CAAGGCCTTG AGAGAGGCCC ACGATGCCTT CCGCTCCTCG 
CTGAGCTCAG CCCAGGCCGA CTTCAACCAG CTGGCTGAGC TGGACCGCCA GATCAAGAGC 
TTCCGCGTGG CCTCCAACCC CTACACCTGG TTCACCATGG AGGCCCTGGA GGAGACCTGG 
AGGAACCTTC AGAAGATCAT CAAGGAAAGG GAGCTGGAGC TGCAGAAGGA GCAGCGGCGG 
CAGGAGGAGA ACGACAAGCT GCGCCAGGAG TTCGCCCAGC ATGCCAACGC CTTCCACCAG 
TGGATCCAGG AGACCAGAAC GTATCTCCTC GATGGCATAG CGTATCGTCG GGTCATTCGT 
GTCTATCAGT ATGAAGTTGG GGATGATCTG TCTGGAAGGT CCTGCATGGT GGAAGAATCT 
GGGACCCTTG AATCGCAGCT TGAAGCTACC AAACGCAAAC ACCAGGAGAT TCGAGCCATG 
AGAAGTCAGC TCAAGAAGAT TGAAGACCTG GGGGCGGCCA TGGAGGAAGC GCTGATCCTG 
GACAACAAGT ACACGGAGCA CAGCACCGTG GGCCTGGCCC AGCAGTGGGA CCAGCTCGAC 
CAGCTGGGCA TGCGGATGCA GCACAACCTG GAGCAGCAGA TCCAGGCCAG GAACACGACA 
GGAGTGACCG AGGAGGCCCT CAAAGAATTC AGCATGATGT TCAAACACTT CGACAAGGAC 
AAGTCCGGCC GGCTGAATCA CCAGGAGTTC AAATCCTGTC TGCGCTCCCT GGGCTATGAC 
CTGCCCATGG TGGAGGAAGG GGAGCCCGAC CCTGAGTTCG AGGCCATCTT GGATACTGTG 
GATCCTAACA GGGACGGCCA CGTCTCCCTG CAAGAGTACA TGGCCTTTAT GATCAGCCGT 
GAGACCGAGA ATGTCAAGTC CAGCGAGGAG ATTGAGAGCG CCTTCCGGGC CCTGAGCTCC 
GAAGGGAAGC CTTATGTGAC CAAGGAGGAG CTGTACCAGA ACCTGACTCG GGAACAAGCC 
GACTACTGCG TCTCCCACAT GAAGCCCTAC GTGGACGGCA AGGGCCGCGA ACTCCCCACT 
GCTTTCGACT ACGTTGAGTT CACCCGCTCG CTCTTCGTGA ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTAN1 ( XM_027966358.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_027822159.1 Ovis aries spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X5, mRNA.

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CloneID	OSm76652
Clone ID Related Accession (Same CDS sequence)	XM_027966358.1
Accession Version	XM_027966358.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7473bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-05
Organism	Ovis aries(sheep)
Product	spectrin alpha chain, non-erythrocytic 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040254.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ovis aries Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTCAT CGTTTCGTAA ACGCAGAGTC CAGAAAATGG ACCCAAGCGG GGTCAAAGTG 
CTGGAGACAG CCGAGGACAT CCAGGAGAGG CGGCAGCAGG TCCTGGACAG ATACCACCGC 
TTCAAGGAGC TCTCGACCCT GAGGCGCCAG AAGCTTGAAG ACTCCTACCG ATTCCAGTTT 
TTCCAAAGAG ATGCTGAGGA GCTGGAGAAA TGGATTCAGG AGAAGCTTCA GATTGCCTCG 
GATGAGAATT ACAAAGACCC AACCAACCTG CAGGGCAAGC TGCAGAAGCA TCAAGCCTTC 
GAAGCCGAGG TGCAGGCCAA CTCGGGTGCC ATCGTGAAGC TTGATGAGAC CGGTAACCTG 
ATGATCTCAG AAGGGCACTT CGCGTCCGAA ACCATCCGGA CTCGTCTGAT GGAGCTGCAC 
CGCCAGTGGG AATTGCTTCT GGAGAAGATG CGGGAGAAAG GCATCAAGCT GCTGCAGGCC 
CAGAAGCTGG TGCAGTACCT GCGGGAGTGT GAGGACGTGA TGGACTGGAT CAATGACAAG 
GAAGCCATTG TGACATCTGA GGAGCTGGGC CAGGACCTGG AGCACGTGGA GGTGTTACAG 
AAGAAATTCG AAGAGTTTCA GACAGACATG GCTGCCCACG AAGAGAGAGT CAATGAAGTG 
AACCAGTTTG CTGCCAAGCT CATTCAGGAG CAGCACCCTG AGGAGGAGCT GATCAAGACA 
AAGGAGGACG AGGTGAATGC AGCCTGGCAG CGGCTGAAGG GCCTGGCCCT CCAGCGGCAG 
GGGAAGCTGT TCGGGGCAGC TGAGGTCCAA CGCTTTAACA GGGATGTGGA CGAGACCATT 
AGCTGGATTA AGGAGAAGGA ACAGTTAATG GCCTCTGATG ACTTTGGCCG GGACCTGGCC 
AGTGTCCAGG CTCTACTGCG GAAACACGAG GGTTTGGAGA GAGATCTTGC TGCCTTGGAG 
GACAAGGTCA AAGCCCTGTG TGCCGAGGCT GACCGCCTGC AGCAGTCCCA CCCTCTGAGT 
GCCACCCAGA TCCAAGTGAA GCGAGAGGAG CTGATCACCA ACTGGGAGCA GATCCGCACC 
CTGGCCGCGG AGAGACACGC ACGTCTCAAC GATTCCTACA GGCTTCAGCG CTTCCTTGCT 
GACTTCCGAG ACCTCACCAG CTGGGTCACT GAGATGAAAG CCCTCATCAA TGCAGATGAA 
CTTGCCAATG ATGTGGCCGG GGCCGAAGCC CTGCTGGATA GGCATCAGGA GCACAAGGGT 
GAAATTGACG CTCATGAAGA TAGCTTTAAA TCTGCAGATG AGTCTGGACA GGCCCTGCTT 
GCAGCTGGTC ACTATGCCTC AGATGAAGTG AGGGAGAAGC TGACCATCCT GTCCGAGGAG 
CGGGCCGCGC TACTAGAGCT CTGGGAGCTG CGACGGCAGC AATATGAGCA GTGCATGGAC 
CTGCAGCTCT TCTACCGCGA CACGGAGCAG GTGGACAACT GGATGAGCAA ACAGGAGGCG 
TTCCTGTTGA ACGAAGACCT GGGTGATTCC TTGGATAGTG TGGAAGCGCT TCTCAAGAAG 
CACGAAGACT TTGAGAAGTC CCTCAGCGCC CAGGAGGAGA AGATCACAGC CTTAGATGAG 
TTTGCCACCA AGCTGATTCA GAACAACCAC TACGCGATGG AAGATGTGGC CACCCGGCGA 
GACGCACTGC TGAGCCGCCG CAACGCCCTC CATGAGAGAG CCATGTACCG CCGTGCCCAG 
CTTGCAGATT CCTTCCACTT GCAGCAGTTT TTCCGTGACT CTGATGAGCT CAAGAGTTGG 
GTCAACGAGA AGATGAAAAC AGCCACAGAT GAAGCTTATA AAGACCCATC CAACCTACAA 
GGGAAAGTGC AGAAGCACCA GGCCTTTGAG GCCGAGCTCT CAGCAAACCA GAGCCGCATC 
GACGCCTTGG AGAAAGCTGG GCAGAAGCTG ATCGACGTTG ACCACTATGC CAAGGATGAG 
GTGGCGACTC GGATGAATGA AGTTATCAGT CTGTGGAAGA AGCTGCTGGA GGCCACGGAG 
CTGAAAGGCA TAAAGCTCCG TGAGGCTAAC CAGCAGCAGC AGTTTAACCG CAACGTTGAG 
GACATCGAGC TGTGGCTGTA CGAAGTGGAA GGTCATCTGG CCTCGGATGA CTACGGCAAA 
GACCTCACCA ACGTCCAGAA CCTCCAGAAA AAGCATGCCC TGCTGGAGGC TGACGTGGCC 
GCTCACCAGG ATCGCATCGA TGGCATCACT ATCCAAGCCC GCCAGTTCCA GGATGCGGGC 
CACTTTGATG CTGAAAACAT CAAGAAGAAG CAGGAGGCCC TGGTAGCTCG CTACGAGGCA 
CTCAAGGAGC CCATGGTTGC CCGGAAGCAG AAGCTGGCCG ATTCCCTGCG GTTGCAGCAG 
CTCTTCCGTG ACGTTGAGGA TGAGGAGACG TGGATTCGGG AGAAAGAACC TATTGCCGCC 
TCCACCAACA GAGGCAAAGA TTTAATTGGA GTCCAGAACC TGCTAAAGAA ACACCAAGCG 
CTACAAGCAG AAATTGCTGG CCACGAGCCC CGCATCAAAG CAGTCACACA GAAGGGGAAC 
GCCATGGTAG AGGAGGGCCA TTTTGCTGCC GAGGACGTGA AGGCCAAGCT CCATGAACTG 
AACCAGAAGT GGGAGTCGCT GAAGTCCAAG GCCTCCCAGC GACGGCAGGA CCTGGAGGAC 
TCGCTGCAGG CCCAGCAGTA CTTCGCTGAT GCCAACGAGG CCGAGTCCTG GATGCGGGAG 
AAGGAGCCCA TCGTGGGCAG CACCGACTAT GGCAAAGATG AGGACTCTGC CGAGGCTCTG 
CTAAAGAAGC ACGAGGCATT GATGTCGGAT CTCAGCGCCT ATGGCAGCAG CATCCAGGCC 
CTGCGAGAGC AGGCCCAGTC GTGCCGGCAA CAAGTGGCCC CCATGGATGA CGAGACTGGG 
AAGGAGCTGG TCTTGGCGCT CTATGACTAT CAGGAGAAGA GTCCCCGAGA GGTCACCATG 
AAGAAAGGAG ACATTCTCAC CTTACTCAAC AGCACCAACA AGGACTGGTG GAAAGTGGAA 
GTGAACGACC GGCAGGGCTT CGTGCCGGCC GCCTACGTGA AGAAGCTGGA CCCAGCGCAG 
TCGGCCTCCC GGGAGAACCT CCTGGAAGAG CAGGGCAGCA TCGCGCTGCG GCAGGAGCAG 
ATTGACAACC AGTACCATTC TCTGCTGGAA CTGGGAGAGA AGCGTAAAGG CATGTTGGAG 
AAGAGCTGTA AGAAGTTCAT GCTGTTCCGT GAAGCGAACG AGCTGCAGCA GTGGATCAAC 
GAGAAGGAAG CGGCCCTGAC AAGCGAGGAG GTCGGCGCGG ACTTGGAGCA GGTCGAAGTG 
CTGCAGAAGA AGTTTGACGA CTTCCAGAAG GATCTCAAGG CCAATGAGTC ACGGCTGAAG 
GACATTAACA AGGTGGCTGA AGACCTGGAG TCGGAAGGCC TGATGGCGGA GGAGGTGCAG 
GCCGTGCAGC AGCAGGAGGT GTATGGCGCG ATGCCCAGGG ATGAATCTGA TTCCAAGACA 
GCCTCCCCGT GGAAGTCTGC TCGCCTGATG GTTCACACCG TGGCCACCTT TAATTCCATC 
AAGGAGCTCA ATGAGCGCTG GCGGTCCCTG CAGCAGCTGG CTGAGGAGCG GAGCCAACTC 
TTGGGCAGCG CCCACGAAGT GCAGCGGTTC CACAGAGATG CTGACGAAAC CAAAGAGTGG 
ATCGAGGAGA AGAACCAGGC TCTAAACACA GACAACTATG GCCACGACCT GGCCAGCGTC 
CAGGCCCTGC AGCGCAAGCA TGAGGGCTTC GAGAGGGACC TCGCGGCCCT TGGCGACAAG 
GTGAACTCGC TCGGGGAGAC AGCCGAGCGC CTGATCCAGT CCCACCCCGA GTCGGCAGAG 
GACCTGCAGG AGAAGTGCAC TGAGCTGAAC CAGGCATGGA GCAGCCTGGG CAAGCGTGCC 
GACCAGCGCA AGGCCAAGCT GGGCGACTCC CACGACCTGC AGCGCTTCCT CAGCGACTTC 
CGGGACCTCA TGTCTTGGAT CAATGGAATC CGAGGTCTGG TGTCCTCAGA CGAGCTAGCC 
AAGGATGTCA CTGGAGCTGA GGCCCTGCTG GAGCGACACC AGGAACACCG GACAGAAATT 
GATGCCAGAG CTGGCACTTT CCAGGCATTC GAGCAGTTTG GGCAGCAGCT GTTGGCTCAT 
GGGCACTACG CCAGCCCCGA GATCAAGGAG AAGCTCGATG TTCTAGACCA GGAGCGCGCA 
GACCTCGAGA AGGCCTGGGT CCAGCGCAGG ATGATGCTGG ATCAGTGCCT TGAATTGCAG 
CTGTTCCATC GGGATTGCGA GCAGGCCGAG AACTGGATGG CTGCCCGGGA GGCCTTCCTG 
AACACAGAAG ACAAAGGGGA CTCGCTAGAC AGCGTGGAGG CTCTGATCAA AAAACACGAG 
GACTTTGACA AAGCAATTAA TGTCCAGGAG GAGAAGATTG CTGCCCTGCA GTCCTTTGCT 
GACCAGCTCA TTGCTGGCGG TCACTACGCC AAAGGCGACA TCTCCAGCCG GCGCAATGAG 
GTTTTGGACA GGTGGCGCCG TCTAAAAGCT CAGATGATTG AGAAGAGGTC AAAGCTGGGA 
GAATCGCAGA CCCTCCAGCA GTTCAGCCGG GATGTAGACG AGATTGAAGC TTGGATCAGT 
GAAAAATTAC AGACTGCAAG TGATGAGTCA TATAAGGACC CCACCAACAT CCAGCTTTCC 
AAGCTGCTGA GCAAGCATCA GAAGCACCAG GCCTTCGAGG CAGAACTGCA CGCCAATGCG 
GACCGGATCC GCGGGGTCAT CGACGTGGGC AACTCCCTCA TTGACCGTGG GGCCTGTGCC 
GGCAGCGAGG ATGCCGTCAA GGCCCGGCTG GCCGCCTTAG CTGACCAGTG GCAGTTCCTG 
GTGCAGAAGT CGGCTGAGAA GAGCCAGAAG CTGAAAGAAG CCAACAAACA GCAGAACTTC 
AACACTGGCA TCAAGGACTT CGACTTCTGG CTCTCTGAGG TGGAAGCCCT GCTGGCCTCT 
GAGGATTATG GCAAAGACCT GGCTTCTGTG AACAACTTGC TAAAAAAACA TCAGCTGCTG 
GAGGCAGATA TCTCTGCCCA CGAGGACCGC CTGAAGGACC TCAACAGCCA GGCGGACAGC 
CTGATGACCA GCAGCGCCTT CGACACCTCC CAGGTGAAGG ATAAGCGGGA CACCATCAAC 
AGCCGTTTCC AGAAGATCAA GAGCATGGCC GCCTCCCGGC GAGCTAGGCT GAATGAGTCC 
CACCGCCTGC ACCAGTTCTT CCGGGACATG GACGATGAGG AGTCCTGGAT CAAGGAGAAG 
AAGCTGCTGG TGAGCTCAGA GGACTACGGC CGCGACCTGA CGGGCGTCCA GAACCTGAGG 
AAGAAGCACA AACGGCTGGA GGCAGAGCTG GCTGCCCACG AGCCGGCCAT TCAGGGTGTC 
CTGGACACTG GAAAGAAGCT GTCTGATGAC AACACCATCG GGAAGGAGGA GATTCAGCAG 
CGCCTGGCGC AGTTTGTGGA GCACTGGAAG GAGCTGAAGC AGCTGGCAGC TGCTCGGGGC 
CAGCGGCTAG AAGAATCTTT GGAATATCAG CAGTTTGTGG CCAACGTGGA GGAGGAGGAA 
GCCTGGATCA ATGAGAAGAT GACCCTGGTG GCCAGTGAGG ATTACGGAGA CACTCTTGCT 
GCCATCCAGG GCTTGCTGAA GAAACACGAA GCCTTCGAGA CGGACTTCAC TGTCCACAAG 
GACCGGGTGA ATGACGTCTG CACTAACGGA CAAGACCTGG TTAAGAAGAA CAATCACCAT 
GAGGAGAACA TCTCTTCCAA GATGAAGGGC TTGAACGGGA AAGTGTCAGA CTTGGAGAAG 
GCTGCCTCCC AGAGGAAGGC AAAGCTCGAC GAGAACTCGG CCTTCCTCCA GTTCAACTGG 
AAGGCCGATG TGGTGGAGTC CTGGATTGGT GAAAAGGAGA ACAGCTTGAA GACAGATGAT 
TATGGTCGAG ATCTGTCTTC TGTCCAAACT CTCCTCACCA AACAGGAAAC CTTCGATGCT 
GGCCTGCAGG CCTTCCAGCA GGAGGGCATC GCCAACATCA CTGCCCTCAA AGATCAGCTG 
CTGGCCGCCA AGCATGTTCA GTCCAAGGCC ATCGAGGCCC GCCACGCTGC CCTCATGAAG 
AGATGGAGCC AGCTCCTGGC CAATTCGGCC ACCCGGAAGA AGAAGCTGCT GGAGGCACAG 
AGTCACTTCC GCAAGGTGGA GGATCTCTTC CTGACCTTTG CCAAAAAGGC TTCAGCCTTC 
AACAGCTGGT TTGAAAATGC AGAGGAGGAC TTAACAGACC CAGTGCGCTG CAACTCCCTG 
GAAGAGATCA AGGCCTTGAG AGAGGCCCAC GATGCCTTCC GCTCCTCGCT GAGCTCAGCC 
CAGGCCGACT TCAACCAGCT GGCTGAGCTG GACCGCCAGA TCAAGAGCTT CCGCGTGGCC 
TCCAACCCCT ACACCTGGTT CACCATGGAG GCCCTGGAGG AGACCTGGAG GAACCTTCAG 
AAGATCATCA AGGAAAGGGA GCTGGAGCTG CAGAAGGAGC AGCGGCGGCA GGAGGAGAAC 
GACAAGCTGC GCCAGGAGTT CGCCCAGCAT GCCAACGCCT TCCACCAGTG GATCCAGGAG 
ACCAGAACGT ATCTCCTCGA TGGCATAGCG TATCGTCGGG TCATTCGTGT CTATCAGTAT 
GAAGTTGGGG ATGATCTGTC TGGAAGGTCC TGCATGGTGG AAGAATCTGG GACCCTTGAA 
TCGCAGCTTG AAGCTACCAA ACGCAAACAC CAGGAGATTC GAGCCATGAG AAGTCAGCTC 
AAGAAGATTG AAGACCTGGG GGCGGCCATG GAGGAAGCGC TGATCCTGGA CAACAAGTAC 
ACGGAGCACA GCACCGTGGG CCTGGCCCAG CAGTGGGACC AGCTCGACCA GCTGGGCATG 
CGGATGCAGC ACAACCTGGA GCAGCAGATC CAGGCCAGGA ACACGACAGG AGTGACCGAG 
GAGGCCCTCA AAGAATTCAG CATGATGTTC AAACACTTCG ACAAGGACAA GTCCGGCCGG 
CTGAATCACC AGGAGTTCAA ATCCTGTCTG CGCTCCCTGG GCTATGACCT GCCCATGGTG 
GAGGAAGGGG AGCCCGACCC TGAGTTCGAG GCCATCTTGG ATACTGTGGA TCCTAACAGG 
GACGGCCACG TCTCCCTGCA AGAGTACATG GCCTTTATGA TCAGCCGTGA GACCGAGAAT 
GTCAAGTCCA GCGAGGAGAT TGAGAGCGCC TTCCGGGCCC TGAGCTCCGA AGGGAAGCCT 
TATGTGACCA AGGAGGAGCT GTACCAGAAC CTGACTCGGG AACAAGCCGA CTACTGCGTC 
TCCCACATGA AGCCCTACGT GGACGGCAAG GGCCGCGAAC TCCCCACTGC TTTCGACTAC 
GTTGAGTTCA CCCGCTCGCT CTTCGTGAAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_027822159.1
CDS	71..7543
Translation	MLSSFRKRRVQKMDPSGVKVLETAEDIQERRQQVLDRYHRFKELSTLRRQKLEDSYRFQFFQRDAEELEKWIQEKLQIASDENYKDPTNLQGKLQKHQAFEAEVQANSGAIVKLDETGNLMISEGHFASETIRTRLMELHRQWELLLEKMREKGIKLLQAQKLVQYLRECEDVMDWINDKEAIVTSEELGQDLEHVEVLQKKFEEFQTDMAAHEERVNEVNQFAAKLIQEQHPEEELIKTKEDEVNAAWQRLKGLALQRQGKLFGAAEVQRFNRDVDETISWIKEKEQLMASDDFGRDLASVQALLRKHEGLERDLAALEDKVKALCAEADRLQQSHPLSATQIQVKREELITNWEQIRTLAAERHARLNDSYRLQRFLADFRDLTSWVTEMKALINADELANDVAGAEALLDRHQEHKGEIDAHEDSFKSADESGQALLAAGHYASDEVREKLTILSEERAALLELWELRRQQYEQCMDLQLFYRDTEQVDNWMSKQEAFLLNEDLGDSLDSVEALLKKHEDFEKSLSAQEEKITALDEFATKLIQNNHYAMEDVATRRDALLSRRNALHERAMYRRAQLADSFHLQQFFRDSDELKSWVNEKMKTATDEAYKDPSNLQGKVQKHQAFEAELSANQSRIDALEKAGQKLIDVDHYAKDEVATRMNEVISLWKKLLEATELKGIKLREANQQQQFNRNVEDIELWLYEVEGHLASDDYGKDLTNVQNLQKKHALLEADVAAHQDRIDGITIQARQFQDAGHFDAENIKKKQEALVARYEALKEPMVARKQKLADSLRLQQLFRDVEDEETWIREKEPIAASTNRGKDLIGVQNLLKKHQALQAEIAGHEPRIKAVTQKGNAMVEEGHFAAEDVKAKLHELNQKWESLKSKASQRRQDLEDSLQAQQYFADANEAESWMREKEPIVGSTDYGKDEDSAEALLKKHEALMSDLSAYGSSIQALREQAQSCRQQVAPMDDETGKELVLALYDYQEKSPREVTMKKGDILTLLNSTNKDWWKVEVNDRQGFVPAAYVKKLDPAQSASRENLLEEQGSIALRQEQIDNQYHSLLELGEKRKGMLEKSCKKFMLFREANELQQWINEKEAALTSEEVGADLEQVEVLQKKFDDFQKDLKANESRLKDINKVAEDLESEGLMAEEVQAVQQQEVYGAMPRDESDSKTASPWKSARLMVHTVATFNSIKELNERWRSLQQLAEERSQLLGSAHEVQRFHRDADETKEWIEEKNQALNTDNYGHDLASVQALQRKHEGFERDLAALGDKVNSLGETAERLIQSHPESAEDLQEKCTELNQAWSSLGKRADQRKAKLGDSHDLQRFLSDFRDLMSWINGIRGLVSSDELAKDVTGAEALLERHQEHRTEIDARAGTFQAFEQFGQQLLAHGHYASPEIKEKLDVLDQERADLEKAWVQRRMMLDQCLELQLFHRDCEQAENWMAAREAFLNTEDKGDSLDSVEALIKKHEDFDKAINVQEEKIAALQSFADQLIAGGHYAKGDISSRRNEVLDRWRRLKAQMIEKRSKLGESQTLQQFSRDVDEIEAWISEKLQTASDESYKDPTNIQLSKLLSKHQKHQAFEAELHANADRIRGVIDVGNSLIDRGACAGSEDAVKARLAALADQWQFLVQKSAEKSQKLKEANKQQNFNTGIKDFDFWLSEVEALLASEDYGKDLASVNNLLKKHQLLEADISAHEDRLKDLNSQADSLMTSSAFDTSQVKDKRDTINSRFQKIKSMAASRRARLNESHRLHQFFRDMDDEESWIKEKKLLVSSEDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQFVEHWKELKQLAAARGQRLEESLEYQQFVANVEEEEAWINEKMTLVASEDYGDTLAAIQGLLKKHEAFETDFTVHKDRVNDVCTNGQDLVKKNNHHEENISSKMKGLNGKVSDLEKAASQRKAKLDENSAFLQFNWKADVVESWIGEKENSLKTDDYGRDLSSVQTLLTKQETFDAGLQAFQQEGIANITALKDQLLAAKHVQSKAIEARHAALMKRWSQLLANSATRKKKLLEAQSHFRKVEDLFLTFAKKASAFNSWFENAEEDLTDPVRCNSLEEIKALREAHDAFRSSLSSAQADFNQLAELDRQIKSFRVASNPYTWFTMEALEETWRNLQKIIKERELELQKEQRRQEENDKLRQEFAQHANAFHQWIQETRTYLLDGIAYRRVIRVYQYEVGDDLSGRSCMVEESGTLESQLEATKRKHQEIRAMRSQLKKIEDLGAAMEEALILDNKYTEHSTVGLAQQWDQLDQLGMRMQHNLEQQIQARNTTGVTEEALKEFSMMFKHFDKDKSGRLNHQEFKSCLRSLGYDLPMVEEGEPDPEFEAILDTVDPNRDGHVSLQEYMAFMISRETENVKSSEEIESAFRALSSEGKPYVTKEELYQNLTREQADYCVSHMKPYVDGKGRELPTAFDYVEFTRSLFVN

Target ORF information:

RefSeq Version	XM_027966358.1
Organism	Ovis aries(sheep)
Definition	Ovis aries spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027966358.1

ORF Insert Sequence:

ATGCTGTCAT CGTTTCGTAA ACGCAGAGTC CAGAAAATGG ACCCAAGCGG GGTCAAAGTG 
CTGGAGACAG CCGAGGACAT CCAGGAGAGG CGGCAGCAGG TCCTGGACAG ATACCACCGC 
TTCAAGGAGC TCTCGACCCT GAGGCGCCAG AAGCTTGAAG ACTCCTACCG ATTCCAGTTT 
TTCCAAAGAG ATGCTGAGGA GCTGGAGAAA TGGATTCAGG AGAAGCTTCA GATTGCCTCG 
GATGAGAATT ACAAAGACCC AACCAACCTG CAGGGCAAGC TGCAGAAGCA TCAAGCCTTC 
GAAGCCGAGG TGCAGGCCAA CTCGGGTGCC ATCGTGAAGC TTGATGAGAC CGGTAACCTG 
ATGATCTCAG AAGGGCACTT CGCGTCCGAA ACCATCCGGA CTCGTCTGAT GGAGCTGCAC 
CGCCAGTGGG AATTGCTTCT GGAGAAGATG CGGGAGAAAG GCATCAAGCT GCTGCAGGCC 
CAGAAGCTGG TGCAGTACCT GCGGGAGTGT GAGGACGTGA TGGACTGGAT CAATGACAAG 
GAAGCCATTG TGACATCTGA GGAGCTGGGC CAGGACCTGG AGCACGTGGA GGTGTTACAG 
AAGAAATTCG AAGAGTTTCA GACAGACATG GCTGCCCACG AAGAGAGAGT CAATGAAGTG 
AACCAGTTTG CTGCCAAGCT CATTCAGGAG CAGCACCCTG AGGAGGAGCT GATCAAGACA 
AAGGAGGACG AGGTGAATGC AGCCTGGCAG CGGCTGAAGG GCCTGGCCCT CCAGCGGCAG 
GGGAAGCTGT TCGGGGCAGC TGAGGTCCAA CGCTTTAACA GGGATGTGGA CGAGACCATT 
AGCTGGATTA AGGAGAAGGA ACAGTTAATG GCCTCTGATG ACTTTGGCCG GGACCTGGCC 
AGTGTCCAGG CTCTACTGCG GAAACACGAG GGTTTGGAGA GAGATCTTGC TGCCTTGGAG 
GACAAGGTCA AAGCCCTGTG TGCCGAGGCT GACCGCCTGC AGCAGTCCCA CCCTCTGAGT 
GCCACCCAGA TCCAAGTGAA GCGAGAGGAG CTGATCACCA ACTGGGAGCA GATCCGCACC 
CTGGCCGCGG AGAGACACGC ACGTCTCAAC GATTCCTACA GGCTTCAGCG CTTCCTTGCT 
GACTTCCGAG ACCTCACCAG CTGGGTCACT GAGATGAAAG CCCTCATCAA TGCAGATGAA 
CTTGCCAATG ATGTGGCCGG GGCCGAAGCC CTGCTGGATA GGCATCAGGA GCACAAGGGT 
GAAATTGACG CTCATGAAGA TAGCTTTAAA TCTGCAGATG AGTCTGGACA GGCCCTGCTT 
GCAGCTGGTC ACTATGCCTC AGATGAAGTG AGGGAGAAGC TGACCATCCT GTCCGAGGAG 
CGGGCCGCGC TACTAGAGCT CTGGGAGCTG CGACGGCAGC AATATGAGCA GTGCATGGAC 
CTGCAGCTCT TCTACCGCGA CACGGAGCAG GTGGACAACT GGATGAGCAA ACAGGAGGCG 
TTCCTGTTGA ACGAAGACCT GGGTGATTCC TTGGATAGTG TGGAAGCGCT TCTCAAGAAG 
CACGAAGACT TTGAGAAGTC CCTCAGCGCC CAGGAGGAGA AGATCACAGC CTTAGATGAG 
TTTGCCACCA AGCTGATTCA GAACAACCAC TACGCGATGG AAGATGTGGC CACCCGGCGA 
GACGCACTGC TGAGCCGCCG CAACGCCCTC CATGAGAGAG CCATGTACCG CCGTGCCCAG 
CTTGCAGATT CCTTCCACTT GCAGCAGTTT TTCCGTGACT CTGATGAGCT CAAGAGTTGG 
GTCAACGAGA AGATGAAAAC AGCCACAGAT GAAGCTTATA AAGACCCATC CAACCTACAA 
GGGAAAGTGC AGAAGCACCA GGCCTTTGAG GCCGAGCTCT CAGCAAACCA GAGCCGCATC 
GACGCCTTGG AGAAAGCTGG GCAGAAGCTG ATCGACGTTG ACCACTATGC CAAGGATGAG 
GTGGCGACTC GGATGAATGA AGTTATCAGT CTGTGGAAGA AGCTGCTGGA GGCCACGGAG 
CTGAAAGGCA TAAAGCTCCG TGAGGCTAAC CAGCAGCAGC AGTTTAACCG CAACGTTGAG 
GACATCGAGC TGTGGCTGTA CGAAGTGGAA GGTCATCTGG CCTCGGATGA CTACGGCAAA 
GACCTCACCA ACGTCCAGAA CCTCCAGAAA AAGCATGCCC TGCTGGAGGC TGACGTGGCC 
GCTCACCAGG ATCGCATCGA TGGCATCACT ATCCAAGCCC GCCAGTTCCA GGATGCGGGC 
CACTTTGATG CTGAAAACAT CAAGAAGAAG CAGGAGGCCC TGGTAGCTCG CTACGAGGCA 
CTCAAGGAGC CCATGGTTGC CCGGAAGCAG AAGCTGGCCG ATTCCCTGCG GTTGCAGCAG 
CTCTTCCGTG ACGTTGAGGA TGAGGAGACG TGGATTCGGG AGAAAGAACC TATTGCCGCC 
TCCACCAACA GAGGCAAAGA TTTAATTGGA GTCCAGAACC TGCTAAAGAA ACACCAAGCG 
CTACAAGCAG AAATTGCTGG CCACGAGCCC CGCATCAAAG CAGTCACACA GAAGGGGAAC 
GCCATGGTAG AGGAGGGCCA TTTTGCTGCC GAGGACGTGA AGGCCAAGCT CCATGAACTG 
AACCAGAAGT GGGAGTCGCT GAAGTCCAAG GCCTCCCAGC GACGGCAGGA CCTGGAGGAC 
TCGCTGCAGG CCCAGCAGTA CTTCGCTGAT GCCAACGAGG CCGAGTCCTG GATGCGGGAG 
AAGGAGCCCA TCGTGGGCAG CACCGACTAT GGCAAAGATG AGGACTCTGC CGAGGCTCTG 
CTAAAGAAGC ACGAGGCATT GATGTCGGAT CTCAGCGCCT ATGGCAGCAG CATCCAGGCC 
CTGCGAGAGC AGGCCCAGTC GTGCCGGCAA CAAGTGGCCC CCATGGATGA CGAGACTGGG 
AAGGAGCTGG TCTTGGCGCT CTATGACTAT CAGGAGAAGA GTCCCCGAGA GGTCACCATG 
AAGAAAGGAG ACATTCTCAC CTTACTCAAC AGCACCAACA AGGACTGGTG GAAAGTGGAA 
GTGAACGACC GGCAGGGCTT CGTGCCGGCC GCCTACGTGA AGAAGCTGGA CCCAGCGCAG 
TCGGCCTCCC GGGAGAACCT CCTGGAAGAG CAGGGCAGCA TCGCGCTGCG GCAGGAGCAG 
ATTGACAACC AGTACCATTC TCTGCTGGAA CTGGGAGAGA AGCGTAAAGG CATGTTGGAG 
AAGAGCTGTA AGAAGTTCAT GCTGTTCCGT GAAGCGAACG AGCTGCAGCA GTGGATCAAC 
GAGAAGGAAG CGGCCCTGAC AAGCGAGGAG GTCGGCGCGG ACTTGGAGCA GGTCGAAGTG 
CTGCAGAAGA AGTTTGACGA CTTCCAGAAG GATCTCAAGG CCAATGAGTC ACGGCTGAAG 
GACATTAACA AGGTGGCTGA AGACCTGGAG TCGGAAGGCC TGATGGCGGA GGAGGTGCAG 
GCCGTGCAGC AGCAGGAGGT GTATGGCGCG ATGCCCAGGG ATGAATCTGA TTCCAAGACA 
GCCTCCCCGT GGAAGTCTGC TCGCCTGATG GTTCACACCG TGGCCACCTT TAATTCCATC 
AAGGAGCTCA ATGAGCGCTG GCGGTCCCTG CAGCAGCTGG CTGAGGAGCG GAGCCAACTC 
TTGGGCAGCG CCCACGAAGT GCAGCGGTTC CACAGAGATG CTGACGAAAC CAAAGAGTGG 
ATCGAGGAGA AGAACCAGGC TCTAAACACA GACAACTATG GCCACGACCT GGCCAGCGTC 
CAGGCCCTGC AGCGCAAGCA TGAGGGCTTC GAGAGGGACC TCGCGGCCCT TGGCGACAAG 
GTGAACTCGC TCGGGGAGAC AGCCGAGCGC CTGATCCAGT CCCACCCCGA GTCGGCAGAG 
GACCTGCAGG AGAAGTGCAC TGAGCTGAAC CAGGCATGGA GCAGCCTGGG CAAGCGTGCC 
GACCAGCGCA AGGCCAAGCT GGGCGACTCC CACGACCTGC AGCGCTTCCT CAGCGACTTC 
CGGGACCTCA TGTCTTGGAT CAATGGAATC CGAGGTCTGG TGTCCTCAGA CGAGCTAGCC 
AAGGATGTCA CTGGAGCTGA GGCCCTGCTG GAGCGACACC AGGAACACCG GACAGAAATT 
GATGCCAGAG CTGGCACTTT CCAGGCATTC GAGCAGTTTG GGCAGCAGCT GTTGGCTCAT 
GGGCACTACG CCAGCCCCGA GATCAAGGAG AAGCTCGATG TTCTAGACCA GGAGCGCGCA 
GACCTCGAGA AGGCCTGGGT CCAGCGCAGG ATGATGCTGG ATCAGTGCCT TGAATTGCAG 
CTGTTCCATC GGGATTGCGA GCAGGCCGAG AACTGGATGG CTGCCCGGGA GGCCTTCCTG 
AACACAGAAG ACAAAGGGGA CTCGCTAGAC AGCGTGGAGG CTCTGATCAA AAAACACGAG 
GACTTTGACA AAGCAATTAA TGTCCAGGAG GAGAAGATTG CTGCCCTGCA GTCCTTTGCT 
GACCAGCTCA TTGCTGGCGG TCACTACGCC AAAGGCGACA TCTCCAGCCG GCGCAATGAG 
GTTTTGGACA GGTGGCGCCG TCTAAAAGCT CAGATGATTG AGAAGAGGTC AAAGCTGGGA 
GAATCGCAGA CCCTCCAGCA GTTCAGCCGG GATGTAGACG AGATTGAAGC TTGGATCAGT 
GAAAAATTAC AGACTGCAAG TGATGAGTCA TATAAGGACC CCACCAACAT CCAGCTTTCC 
AAGCTGCTGA GCAAGCATCA GAAGCACCAG GCCTTCGAGG CAGAACTGCA CGCCAATGCG 
GACCGGATCC GCGGGGTCAT CGACGTGGGC AACTCCCTCA TTGACCGTGG GGCCTGTGCC 
GGCAGCGAGG ATGCCGTCAA GGCCCGGCTG GCCGCCTTAG CTGACCAGTG GCAGTTCCTG 
GTGCAGAAGT CGGCTGAGAA GAGCCAGAAG CTGAAAGAAG CCAACAAACA GCAGAACTTC 
AACACTGGCA TCAAGGACTT CGACTTCTGG CTCTCTGAGG TGGAAGCCCT GCTGGCCTCT 
GAGGATTATG GCAAAGACCT GGCTTCTGTG AACAACTTGC TAAAAAAACA TCAGCTGCTG 
GAGGCAGATA TCTCTGCCCA CGAGGACCGC CTGAAGGACC TCAACAGCCA GGCGGACAGC 
CTGATGACCA GCAGCGCCTT CGACACCTCC CAGGTGAAGG ATAAGCGGGA CACCATCAAC 
AGCCGTTTCC AGAAGATCAA GAGCATGGCC GCCTCCCGGC GAGCTAGGCT GAATGAGTCC 
CACCGCCTGC ACCAGTTCTT CCGGGACATG GACGATGAGG AGTCCTGGAT CAAGGAGAAG 
AAGCTGCTGG TGAGCTCAGA GGACTACGGC CGCGACCTGA CGGGCGTCCA GAACCTGAGG 
AAGAAGCACA AACGGCTGGA GGCAGAGCTG GCTGCCCACG AGCCGGCCAT TCAGGGTGTC 
CTGGACACTG GAAAGAAGCT GTCTGATGAC AACACCATCG GGAAGGAGGA GATTCAGCAG 
CGCCTGGCGC AGTTTGTGGA GCACTGGAAG GAGCTGAAGC AGCTGGCAGC TGCTCGGGGC 
CAGCGGCTAG AAGAATCTTT GGAATATCAG CAGTTTGTGG CCAACGTGGA GGAGGAGGAA 
GCCTGGATCA ATGAGAAGAT GACCCTGGTG GCCAGTGAGG ATTACGGAGA CACTCTTGCT 
GCCATCCAGG GCTTGCTGAA GAAACACGAA GCCTTCGAGA CGGACTTCAC TGTCCACAAG 
GACCGGGTGA ATGACGTCTG CACTAACGGA CAAGACCTGG TTAAGAAGAA CAATCACCAT 
GAGGAGAACA TCTCTTCCAA GATGAAGGGC TTGAACGGGA AAGTGTCAGA CTTGGAGAAG 
GCTGCCTCCC AGAGGAAGGC AAAGCTCGAC GAGAACTCGG CCTTCCTCCA GTTCAACTGG 
AAGGCCGATG TGGTGGAGTC CTGGATTGGT GAAAAGGAGA ACAGCTTGAA GACAGATGAT 
TATGGTCGAG ATCTGTCTTC TGTCCAAACT CTCCTCACCA AACAGGAAAC CTTCGATGCT 
GGCCTGCAGG CCTTCCAGCA GGAGGGCATC GCCAACATCA CTGCCCTCAA AGATCAGCTG 
CTGGCCGCCA AGCATGTTCA GTCCAAGGCC ATCGAGGCCC GCCACGCTGC CCTCATGAAG 
AGATGGAGCC AGCTCCTGGC CAATTCGGCC ACCCGGAAGA AGAAGCTGCT GGAGGCACAG 
AGTCACTTCC GCAAGGTGGA GGATCTCTTC CTGACCTTTG CCAAAAAGGC TTCAGCCTTC 
AACAGCTGGT TTGAAAATGC AGAGGAGGAC TTAACAGACC CAGTGCGCTG CAACTCCCTG 
GAAGAGATCA AGGCCTTGAG AGAGGCCCAC GATGCCTTCC GCTCCTCGCT GAGCTCAGCC 
CAGGCCGACT TCAACCAGCT GGCTGAGCTG GACCGCCAGA TCAAGAGCTT CCGCGTGGCC 
TCCAACCCCT ACACCTGGTT CACCATGGAG GCCCTGGAGG AGACCTGGAG GAACCTTCAG 
AAGATCATCA AGGAAAGGGA GCTGGAGCTG CAGAAGGAGC AGCGGCGGCA GGAGGAGAAC 
GACAAGCTGC GCCAGGAGTT CGCCCAGCAT GCCAACGCCT TCCACCAGTG GATCCAGGAG 
ACCAGAACGT ATCTCCTCGA TGGCATAGCG TATCGTCGGG TCATTCGTGT CTATCAGTAT 
GAAGTTGGGG ATGATCTGTC TGGAAGGTCC TGCATGGTGG AAGAATCTGG GACCCTTGAA 
TCGCAGCTTG AAGCTACCAA ACGCAAACAC CAGGAGATTC GAGCCATGAG AAGTCAGCTC 
AAGAAGATTG AAGACCTGGG GGCGGCCATG GAGGAAGCGC TGATCCTGGA CAACAAGTAC 
ACGGAGCACA GCACCGTGGG CCTGGCCCAG CAGTGGGACC AGCTCGACCA GCTGGGCATG 
CGGATGCAGC ACAACCTGGA GCAGCAGATC CAGGCCAGGA ACACGACAGG AGTGACCGAG 
GAGGCCCTCA AAGAATTCAG CATGATGTTC AAACACTTCG ACAAGGACAA GTCCGGCCGG 
CTGAATCACC AGGAGTTCAA ATCCTGTCTG CGCTCCCTGG GCTATGACCT GCCCATGGTG 
GAGGAAGGGG AGCCCGACCC TGAGTTCGAG GCCATCTTGG ATACTGTGGA TCCTAACAGG 
GACGGCCACG TCTCCCTGCA AGAGTACATG GCCTTTATGA TCAGCCGTGA GACCGAGAAT 
GTCAAGTCCA GCGAGGAGAT TGAGAGCGCC TTCCGGGCCC TGAGCTCCGA AGGGAAGCCT 
TATGTGACCA AGGAGGAGCT GTACCAGAAC CTGACTCGGG AACAAGCCGA CTACTGCGTC 
TCCCACATGA AGCCCTACGT GGACGGCAAG GGCCGCGAAC TCCCCACTGC TTTCGACTAC 
GTTGAGTTCA CCCGCTCGCT CTTCGTGAAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTAN1 ( XM_027966359.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_027822160.1 Ovis aries spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X6, mRNA.

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CloneID	OSm76653
Clone ID Related Accession (Same CDS sequence)	XM_027966359.1
Accession Version	XM_027966359.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7470bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-05
Organism	Ovis aries(sheep)
Product	spectrin alpha chain, non-erythrocytic 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040254.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ovis aries Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTCAT CGTTTCGTAA ACGCAGAGTC CAGAAAATGG ACCCAAGCGG GGTCAAAGTG 
CTGGAGACAG CCGAGGACAT CCAGGAGAGG CGGCAGCAGG TCCTGGACAG ATACCACCGC 
TTCAAGGAGC TCTCGACCCT GAGGCGCCAG AAGCTTGAAG ACTCCTACCG ATTCCAGTTT 
TTCCAAAGAG ATGCTGAGGA GCTGGAGAAA TGGATTCAGG AGAAGCTTCA GATTGCCTCG 
GATGAGAATT ACAAAGACCC AACCAACCTG CAGGGCAAGC TGCAGAAGCA TCAAGCCTTC 
GAAGCCGAGG TGCAGGCCAA CTCGGGTGCC ATCGTGAAGC TTGATGAGAC CGGTAACCTG 
ATGATCTCAG AAGGGCACTT CGCGTCCGAA ACCATCCGGA CTCGTCTGAT GGAGCTGCAC 
CGCCAGTGGG AATTGCTTCT GGAGAAGATG CGGGAGAAAG GCATCAAGCT GCTGCAGGCC 
CAGAAGCTGG TGCAGTACCT GCGGGAGTGT GAGGACGTGA TGGACTGGAT CAATGACAAG 
GAAGCCATTG TGACATCTGA GGAGCTGGGC CAGGACCTGG AGCACGTGGA GGTGTTACAG 
AAGAAATTCG AAGAGTTTCA GACAGACATG GCTGCCCACG AAGAGAGAGT CAATGAAGTG 
AACCAGTTTG CTGCCAAGCT CATTCAGGAG CAGCACCCTG AGGAGGAGCT GATCAAGACA 
AAGGAGGACG AGGTGAATGC AGCCTGGCAG CGGCTGAAGG GCCTGGCCCT CCAGCGGCAG 
GGGAAGCTGT TCGGGGCAGC TGAGGTCCAA CGCTTTAACA GGGATGTGGA CGAGACCATT 
AGCTGGATTA AGGAGAAGGA ACAGTTAATG GCCTCTGATG ACTTTGGCCG GGACCTGGCC 
AGTGTCCAGG CTCTACTGCG GAAACACGAG GGTTTGGAGA GAGATCTTGC TGCCTTGGAG 
GACAAGGTCA AAGCCCTGTG TGCCGAGGCT GACCGCCTGC AGCAGTCCCA CCCTCTGAGT 
GCCACCCAGA TCCAAGTGAA GCGAGAGGAG CTGATCACCA ACTGGGAGCA GATCCGCACC 
CTGGCCGCGG AGAGACACGC ACGTCTCAAC GATTCCTACA GGCTTCAGCG CTTCCTTGCT 
GACTTCCGAG ACCTCACCAG CTGGGTCACT GAGATGAAAG CCCTCATCAA TGCAGATGAA 
CTTGCCAATG ATGTGGCCGG GGCCGAAGCC CTGCTGGATA GGCATCAGGA GCACAAGGGT 
GAAATTGACG CTCATGAAGA TAGCTTTAAA TCTGCAGATG AGTCTGGACA GGCCCTGCTT 
GCAGCTGGTC ACTATGCCTC AGATGAAGTG AGGGAGAAGC TGACCATCCT GTCCGAGGAG 
CGGGCCGCGC TACTAGAGCT CTGGGAGCTG CGACGGCAGC AATATGAGCA GTGCATGGAC 
CTGCAGCTCT TCTACCGCGA CACGGAGCAG GTGGACAACT GGATGAGCAA ACAGGAGGCG 
TTCCTGTTGA ACGAAGACCT GGGTGATTCC TTGGATAGTG TGGAAGCGCT TCTCAAGAAG 
CACGAAGACT TTGAGAAGTC CCTCAGCGCC CAGGAGGAGA AGATCACAGC CTTAGATGAG 
TTTGCCACCA AGCTGATTCA GAACAACCAC TACGCGATGG AAGATGTGGC CACCCGGCGA 
GACGCACTGC TGAGCCGCCG CAACGCCCTC CATGAGAGAG CCATGTACCG CCGTGCCCAG 
CTTGCAGATT CCTTCCACTT GCAGCAGTTT TTCCGTGACT CTGATGAGCT CAAGAGTTGG 
GTCAACGAGA AGATGAAAAC AGCCACAGAT GAAGCTTATA AAGACCCATC CAACCTACAA 
GGGAAAGTGC AGAAGCACCA GGCCTTTGAG GCCGAGCTCT CAGCAAACCA GAGCCGCATC 
GACGCCTTGG AGAAAGCTGG GCAGAAGCTG ATCGACGTTG ACCACTATGC CAAGGATGAG 
GTGGCGACTC GGATGAATGA AGTTATCAGT CTGTGGAAGA AGCTGCTGGA GGCCACGGAG 
CTGAAAGGCA TAAAGCTCCG TGAGGCTAAC CAGCAGCAGC AGTTTAACCG CAACGTTGAG 
GACATCGAGC TGTGGCTGTA CGAAGTGGAA GGTCATCTGG CCTCGGATGA CTACGGCAAA 
GACCTCACCA ACGTCCAGAA CCTCCAGAAA AAGCATGCCC TGCTGGAGGC TGACGTGGCC 
GCTCACCAGG ATCGCATCGA TGGCATCACT ATCCAAGCCC GCCAGTTCCA GGATGCGGGC 
CACTTTGATG CTGAAAACAT CAAGAAGAAG CAGGAGGCCC TGGTAGCTCG CTACGAGGCA 
CTCAAGGAGC CCATGGTTGC CCGGAAGCAG AAGCTGGCCG ATTCCCTGCG GTTGCAGCAG 
CTCTTCCGTG ACGTTGAGGA TGAGGAGACG TGGATTCGGG AGAAAGAACC TATTGCCGCC 
TCCACCAACA GAGGCAAAGA TTTAATTGGA GTCCAGAACC TGCTAAAGAA ACACCAAGCG 
CTACAAGCAG AAATTGCTGG CCACGAGCCC CGCATCAAAG CAGTCACACA GAAGGGGAAC 
GCCATGGTAG AGGAGGGCCA TTTTGCTGCC GAGGACGTGA AGGCCAAGCT CCATGAACTG 
AACCAGAAGT GGGAGTCGCT GAAGTCCAAG GCCTCCCAGC GACGGCAGGA CCTGGAGGAC 
TCGCTGCAGG CCCAGCAGTA CTTCGCTGAT GCCAACGAGG CCGAGTCCTG GATGCGGGAG 
AAGGAGCCCA TCGTGGGCAG CACCGACTAT GGCAAAGATG AGGACTCTGC CGAGGCTCTG 
CTAAAGAAGC ACGAGGCATT GATGTCGGAT CTCAGCGCCT ATGGCAGCAG CATCCAGGCC 
CTGCGAGAGC AGGCCCAGTC GTGCCGGCAA CAAGTGGCCC CCATGGATGA CGAGACTGGG 
AAGGAGCTGG TCTTGGCGCT CTATGACTAT CAGGAGAAGA GTCCCCGAGA GGTCACCATG 
AAGAAAGGAG ACATTCTCAC CTTACTCAAC AGCACCAACA AGGACTGGTG GAAAGTGGAA 
GTGAACGACC GGCAGGGCTT CGTGCCGGCC GCCTACGTGA AGAAGCTGGA CCCAGCGCAG 
TCGGCCTCCC GGGAGAACCT CCTGGAAGAG CAGGGCAGCA TCGCGCTGCG GCAGGAGCAG 
ATTGACAACC AGACGCGCGT AACTAAGGAG GCCGGCAGCG TGTCTCTGCG TATGAAACAG 
GTGGAAGAAC TGTACCATTC TCTGCTGGAA CTGGGAGAGA AGCGTAAAGG CATGTTGGAG 
AAGAGCTGTA AGAAGTTCAT GCTGTTCCGT GAAGCGAACG AGCTGCAGCA GTGGATCAAC 
GAGAAGGAAG CGGCCCTGAC AAGCGAGGAG GTCGGCGCGG ACTTGGAGCA GGTCGAAGTG 
CTGCAGAAGA AGTTTGACGA CTTCCAGAAG GATCTCAAGG CCAATGAGTC ACGGCTGAAG 
GACATTAACA AGGTGGCTGA AGACCTGGAG TCGGAAGGCC TGATGGCGGA GGAGGTGCAG 
GCCGTGCAGC AGCAGGAGGT GTATGGCGCG ATGCCCAGGG ATGAATCTGA TTCCAAGACA 
GCCTCCCCGT GGAAGTCTGC TCGCCTGATG GTTCACACCG TGGCCACCTT TAATTCCATC 
AAGGAGCTCA ATGAGCGCTG GCGGTCCCTG CAGCAGCTGG CTGAGGAGCG GAGCCAACTC 
TTGGGCAGCG CCCACGAAGT GCAGCGGTTC CACAGAGATG CTGACGAAAC CAAAGAGTGG 
ATCGAGGAGA AGAACCAGGC TCTAAACACA GACAACTATG GCCACGACCT GGCCAGCGTC 
CAGGCCCTGC AGCGCAAGCA TGAGGGCTTC GAGAGGGACC TCGCGGCCCT TGGCGACAAG 
GTGAACTCGC TCGGGGAGAC AGCCGAGCGC CTGATCCAGT CCCACCCCGA GTCGGCAGAG 
GACCTGCAGG AGAAGTGCAC TGAGCTGAAC CAGGCATGGA GCAGCCTGGG CAAGCGTGCC 
GACCAGCGCA AGGCCAAGCT GGGCGACTCC CACGACCTGC AGCGCTTCCT CAGCGACTTC 
CGGGACCTCA TGTCTTGGAT CAATGGAATC CGAGGTCTGG TGTCCTCAGA CGAGCTAGCC 
AAGGATGTCA CTGGAGCTGA GGCCCTGCTG GAGCGACACC AGGAACACCG GACAGAAATT 
GATGCCAGAG CTGGCACTTT CCAGGCATTC GAGCAGTTTG GGCAGCAGCT GTTGGCTCAT 
GGGCACTACG CCAGCCCCGA GATCAAGGAG AAGCTCGATG TTCTAGACCA GGAGCGCGCA 
GACCTCGAGA AGGCCTGGGT CCAGCGCAGG ATGATGCTGG ATCAGTGCCT TGAATTGCAG 
CTGTTCCATC GGGATTGCGA GCAGGCCGAG AACTGGATGG CTGCCCGGGA GGCCTTCCTG 
AACACAGAAG ACAAAGGGGA CTCGCTAGAC AGCGTGGAGG CTCTGATCAA AAAACACGAG 
GACTTTGACA AAGCAATTAA TGTCCAGGAG GAGAAGATTG CTGCCCTGCA GTCCTTTGCT 
GACCAGCTCA TTGCTGGCGG TCACTACGCC AAAGGCGACA TCTCCAGCCG GCGCAATGAG 
GTTTTGGACA GGTGGCGCCG TCTAAAAGCT CAGATGATTG AGAAGAGGTC AAAGCTGGGA 
GAATCGCAGA CCCTCCAGCA GTTCAGCCGG GATGTAGACG AGATTGAAGC TTGGATCAGT 
GAAAAATTAC AGACTGCAAG TGATGAGTCA TATAAGGACC CCACCAACAT CCAGCTTTCC 
AAGCTGCTGA GCAAGCATCA GAAGCACCAG GCCTTCGAGG CAGAACTGCA CGCCAATGCG 
GACCGGATCC GCGGGGTCAT CGACGTGGGC AACTCCCTCA TTGACCGTGG GGCCTGTGCC 
GGCAGCGAGG ATGCCGTCAA GGCCCGGCTG GCCGCCTTAG CTGACCAGTG GCAGTTCCTG 
GTGCAGAAGT CGGCTGAGAA GAGCCAGAAG CTGAAAGAAG CCAACAAACA GCAGAACTTC 
AACACTGGCA TCAAGGACTT CGACTTCTGG CTCTCTGAGG TGGAAGCCCT GCTGGCCTCT 
GAGGATTATG GCAAAGACCT GGCTTCTGTG AACAACTTGC TAAAAAAACA TCAGCTGCTG 
GAGGCAGATA TCTCTGCCCA CGAGGACCGC CTGAAGGACC TCAACAGCCA GGCGGACAGC 
CTGATGACCA GCAGCGCCTT CGACACCTCC CAGGTGAAGG ATAAGCGGGA CACCATCAAC 
AGCCGTTTCC AGAAGATCAA GAGCATGGCC GCCTCCCGGC GAGCTAGGCT GAATGAGTCC 
CACCGCCTGC ACCAGTTCTT CCGGGACATG GACGATGAGG AGTCCTGGAT CAAGGAGAAG 
AAGCTGCTGG TGAGCTCAGA GGACTACGGC CGCGACCTGA CGGGCGTCCA GAACCTGAGG 
AAGAAGCACA AACGGCTGGA GGCAGAGCTG GCTGCCCACG AGCCGGCCAT TCAGGGTGTC 
CTGGACACTG GAAAGAAGCT GTCTGATGAC AACACCATCG GGAAGGAGGA GATTCAGCAG 
CGCCTGGCGC AGTTTGTGGA GCACTGGAAG GAGCTGAAGC AGCTGGCAGC TGCTCGGGGC 
CAGCGGCTAG AAGAATCTTT GGAATATCAG CAGTTTGTGG CCAACGTGGA GGAGGAGGAA 
GCCTGGATCA ATGAGAAGAT GACCCTGGTG GCCAGTGAGG ATTACGGAGA CACTCTTGCT 
GCCATCCAGG GCTTGCTGAA GAAACACGAA GCCTTCGAGA CGGACTTCAC TGTCCACAAG 
GACCGGGTGA ATGACGTCTG CACTAACGGA CAAGACCTGG TTAAGAAGAA CAATCACCAT 
GAGGAGAACA TCTCTTCCAA GATGAAGGGC TTGAACGGGA AAGTGTCAGA CTTGGAGAAG 
GCTGCCTCCC AGAGGAAGGC AAAGCTCGAC GAGAACTCGG CCTTCCTCCA GTTCAACTGG 
AAGGCCGATG TGGTGGAGTC CTGGATTGGT GAAAAGGAGA ACAGCTTGAA GACAGATGAT 
TATGGTCGAG ATCTGTCTTC TGTCCAAACT CTCCTCACCA AACAGGAAAC CTTCGATGCT 
GGCCTGCAGG CCTTCCAGCA GGAGGGCATC GCCAACATCA CTGCCCTCAA AGATCAGCTG 
CTGGCCGCCA AGCATGTTCA GTCCAAGGCC ATCGAGGCCC GCCACGCTGC CCTCATGAAG 
AGATGGAGCC AGCTCCTGGC CAATTCGGCC ACCCGGAAGA AGAAGCTGCT GGAGGCACAG 
AGTCACTTCC GCAAGGTGGA GGATCTCTTC CTGACCTTTG CCAAAAAGGC TTCAGCCTTC 
AACAGCTGGT TTGAAAATGC AGAGGAGGAC TTAACAGACC CAGTGCGCTG CAACTCCCTG 
GAAGAGATCA AGGCCTTGAG AGAGGCCCAC GATGCCTTCC GCTCCTCGCT GAGCTCAGCC 
CAGGCCGACT TCAACCAGCT GGCTGAGCTG GACCGCCAGA TCAAGAGCTT CCGCGTGGCC 
TCCAACCCCT ACACCTGGTT CACCATGGAG GCCCTGGAGG AGACCTGGAG GAACCTTCAG 
AAGATCATCA AGGAAAGGGA GCTGGAGCTG CAGAAGGAGC AGCGGCGGCA GGAGGAGAAC 
GACAAGCTGC GCCAGGAGTT CGCCCAGCAT GCCAACGCCT TCCACCAGTG GATCCAGGAG 
ACCAGAACGT ATCTCCTCGA TGGGTCCTGC ATGGTGGAAG AATCTGGGAC CCTTGAATCG 
CAGCTTGAAG CTACCAAACG CAAACACCAG GAGATTCGAG CCATGAGAAG TCAGCTCAAG 
AAGATTGAAG ACCTGGGGGC GGCCATGGAG GAAGCGCTGA TCCTGGACAA CAAGTACACG 
GAGCACAGCA CCGTGGGCCT GGCCCAGCAG TGGGACCAGC TCGACCAGCT GGGCATGCGG 
ATGCAGCACA ACCTGGAGCA GCAGATCCAG GCCAGGAACA CGACAGGAGT GACCGAGGAG 
GCCCTCAAAG AATTCAGCAT GATGTTCAAA CACTTCGACA AGGACAAGTC CGGCCGGCTG 
AATCACCAGG AGTTCAAATC CTGTCTGCGC TCCCTGGGCT ATGACCTGCC CATGGTGGAG 
GAAGGGGAGC CCGACCCTGA GTTCGAGGCC ATCTTGGATA CTGTGGATCC TAACAGGGAC 
GGCCACGTCT CCCTGCAAGA GTACATGGCC TTTATGATCA GCCGTGAGAC CGAGAATGTC 
AAGTCCAGCG AGGAGATTGA GAGCGCCTTC CGGGCCCTGA GCTCCGAAGG GAAGCCTTAT 
GTGACCAAGG AGGAGCTGTA CCAGAACCTG ACTCGGGAAC AAGCCGACTA CTGCGTCTCC 
CACATGAAGC CCTACGTGGA CGGCAAGGGC CGCGAACTCC CCACTGCTTT CGACTACGTT 
GAGTTCACCC GCTCGCTCTT CGTGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_027822160.1
CDS	70..7539
Translation	MLSSFRKRRVQKMDPSGVKVLETAEDIQERRQQVLDRYHRFKELSTLRRQKLEDSYRFQFFQRDAEELEKWIQEKLQIASDENYKDPTNLQGKLQKHQAFEAEVQANSGAIVKLDETGNLMISEGHFASETIRTRLMELHRQWELLLEKMREKGIKLLQAQKLVQYLRECEDVMDWINDKEAIVTSEELGQDLEHVEVLQKKFEEFQTDMAAHEERVNEVNQFAAKLIQEQHPEEELIKTKEDEVNAAWQRLKGLALQRQGKLFGAAEVQRFNRDVDETISWIKEKEQLMASDDFGRDLASVQALLRKHEGLERDLAALEDKVKALCAEADRLQQSHPLSATQIQVKREELITNWEQIRTLAAERHARLNDSYRLQRFLADFRDLTSWVTEMKALINADELANDVAGAEALLDRHQEHKGEIDAHEDSFKSADESGQALLAAGHYASDEVREKLTILSEERAALLELWELRRQQYEQCMDLQLFYRDTEQVDNWMSKQEAFLLNEDLGDSLDSVEALLKKHEDFEKSLSAQEEKITALDEFATKLIQNNHYAMEDVATRRDALLSRRNALHERAMYRRAQLADSFHLQQFFRDSDELKSWVNEKMKTATDEAYKDPSNLQGKVQKHQAFEAELSANQSRIDALEKAGQKLIDVDHYAKDEVATRMNEVISLWKKLLEATELKGIKLREANQQQQFNRNVEDIELWLYEVEGHLASDDYGKDLTNVQNLQKKHALLEADVAAHQDRIDGITIQARQFQDAGHFDAENIKKKQEALVARYEALKEPMVARKQKLADSLRLQQLFRDVEDEETWIREKEPIAASTNRGKDLIGVQNLLKKHQALQAEIAGHEPRIKAVTQKGNAMVEEGHFAAEDVKAKLHELNQKWESLKSKASQRRQDLEDSLQAQQYFADANEAESWMREKEPIVGSTDYGKDEDSAEALLKKHEALMSDLSAYGSSIQALREQAQSCRQQVAPMDDETGKELVLALYDYQEKSPREVTMKKGDILTLLNSTNKDWWKVEVNDRQGFVPAAYVKKLDPAQSASRENLLEEQGSIALRQEQIDNQTRVTKEAGSVSLRMKQVEELYHSLLELGEKRKGMLEKSCKKFMLFREANELQQWINEKEAALTSEEVGADLEQVEVLQKKFDDFQKDLKANESRLKDINKVAEDLESEGLMAEEVQAVQQQEVYGAMPRDESDSKTASPWKSARLMVHTVATFNSIKELNERWRSLQQLAEERSQLLGSAHEVQRFHRDADETKEWIEEKNQALNTDNYGHDLASVQALQRKHEGFERDLAALGDKVNSLGETAERLIQSHPESAEDLQEKCTELNQAWSSLGKRADQRKAKLGDSHDLQRFLSDFRDLMSWINGIRGLVSSDELAKDVTGAEALLERHQEHRTEIDARAGTFQAFEQFGQQLLAHGHYASPEIKEKLDVLDQERADLEKAWVQRRMMLDQCLELQLFHRDCEQAENWMAAREAFLNTEDKGDSLDSVEALIKKHEDFDKAINVQEEKIAALQSFADQLIAGGHYAKGDISSRRNEVLDRWRRLKAQMIEKRSKLGESQTLQQFSRDVDEIEAWISEKLQTASDESYKDPTNIQLSKLLSKHQKHQAFEAELHANADRIRGVIDVGNSLIDRGACAGSEDAVKARLAALADQWQFLVQKSAEKSQKLKEANKQQNFNTGIKDFDFWLSEVEALLASEDYGKDLASVNNLLKKHQLLEADISAHEDRLKDLNSQADSLMTSSAFDTSQVKDKRDTINSRFQKIKSMAASRRARLNESHRLHQFFRDMDDEESWIKEKKLLVSSEDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQFVEHWKELKQLAAARGQRLEESLEYQQFVANVEEEEAWINEKMTLVASEDYGDTLAAIQGLLKKHEAFETDFTVHKDRVNDVCTNGQDLVKKNNHHEENISSKMKGLNGKVSDLEKAASQRKAKLDENSAFLQFNWKADVVESWIGEKENSLKTDDYGRDLSSVQTLLTKQETFDAGLQAFQQEGIANITALKDQLLAAKHVQSKAIEARHAALMKRWSQLLANSATRKKKLLEAQSHFRKVEDLFLTFAKKASAFNSWFENAEEDLTDPVRCNSLEEIKALREAHDAFRSSLSSAQADFNQLAELDRQIKSFRVASNPYTWFTMEALEETWRNLQKIIKERELELQKEQRRQEENDKLRQEFAQHANAFHQWIQETRTYLLDGSCMVEESGTLESQLEATKRKHQEIRAMRSQLKKIEDLGAAMEEALILDNKYTEHSTVGLAQQWDQLDQLGMRMQHNLEQQIQARNTTGVTEEALKEFSMMFKHFDKDKSGRLNHQEFKSCLRSLGYDLPMVEEGEPDPEFEAILDTVDPNRDGHVSLQEYMAFMISRETENVKSSEEIESAFRALSSEGKPYVTKEELYQNLTREQADYCVSHMKPYVDGKGRELPTAFDYVEFTRSLFVN

Target ORF information:

RefSeq Version	XM_027966359.1
Organism	Ovis aries(sheep)
Definition	Ovis aries spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027966359.1

ORF Insert Sequence:

ATGCTGTCAT CGTTTCGTAA ACGCAGAGTC CAGAAAATGG ACCCAAGCGG GGTCAAAGTG 
CTGGAGACAG CCGAGGACAT CCAGGAGAGG CGGCAGCAGG TCCTGGACAG ATACCACCGC 
TTCAAGGAGC TCTCGACCCT GAGGCGCCAG AAGCTTGAAG ACTCCTACCG ATTCCAGTTT 
TTCCAAAGAG ATGCTGAGGA GCTGGAGAAA TGGATTCAGG AGAAGCTTCA GATTGCCTCG 
GATGAGAATT ACAAAGACCC AACCAACCTG CAGGGCAAGC TGCAGAAGCA TCAAGCCTTC 
GAAGCCGAGG TGCAGGCCAA CTCGGGTGCC ATCGTGAAGC TTGATGAGAC CGGTAACCTG 
ATGATCTCAG AAGGGCACTT CGCGTCCGAA ACCATCCGGA CTCGTCTGAT GGAGCTGCAC 
CGCCAGTGGG AATTGCTTCT GGAGAAGATG CGGGAGAAAG GCATCAAGCT GCTGCAGGCC 
CAGAAGCTGG TGCAGTACCT GCGGGAGTGT GAGGACGTGA TGGACTGGAT CAATGACAAG 
GAAGCCATTG TGACATCTGA GGAGCTGGGC CAGGACCTGG AGCACGTGGA GGTGTTACAG 
AAGAAATTCG AAGAGTTTCA GACAGACATG GCTGCCCACG AAGAGAGAGT CAATGAAGTG 
AACCAGTTTG CTGCCAAGCT CATTCAGGAG CAGCACCCTG AGGAGGAGCT GATCAAGACA 
AAGGAGGACG AGGTGAATGC AGCCTGGCAG CGGCTGAAGG GCCTGGCCCT CCAGCGGCAG 
GGGAAGCTGT TCGGGGCAGC TGAGGTCCAA CGCTTTAACA GGGATGTGGA CGAGACCATT 
AGCTGGATTA AGGAGAAGGA ACAGTTAATG GCCTCTGATG ACTTTGGCCG GGACCTGGCC 
AGTGTCCAGG CTCTACTGCG GAAACACGAG GGTTTGGAGA GAGATCTTGC TGCCTTGGAG 
GACAAGGTCA AAGCCCTGTG TGCCGAGGCT GACCGCCTGC AGCAGTCCCA CCCTCTGAGT 
GCCACCCAGA TCCAAGTGAA GCGAGAGGAG CTGATCACCA ACTGGGAGCA GATCCGCACC 
CTGGCCGCGG AGAGACACGC ACGTCTCAAC GATTCCTACA GGCTTCAGCG CTTCCTTGCT 
GACTTCCGAG ACCTCACCAG CTGGGTCACT GAGATGAAAG CCCTCATCAA TGCAGATGAA 
CTTGCCAATG ATGTGGCCGG GGCCGAAGCC CTGCTGGATA GGCATCAGGA GCACAAGGGT 
GAAATTGACG CTCATGAAGA TAGCTTTAAA TCTGCAGATG AGTCTGGACA GGCCCTGCTT 
GCAGCTGGTC ACTATGCCTC AGATGAAGTG AGGGAGAAGC TGACCATCCT GTCCGAGGAG 
CGGGCCGCGC TACTAGAGCT CTGGGAGCTG CGACGGCAGC AATATGAGCA GTGCATGGAC 
CTGCAGCTCT TCTACCGCGA CACGGAGCAG GTGGACAACT GGATGAGCAA ACAGGAGGCG 
TTCCTGTTGA ACGAAGACCT GGGTGATTCC TTGGATAGTG TGGAAGCGCT TCTCAAGAAG 
CACGAAGACT TTGAGAAGTC CCTCAGCGCC CAGGAGGAGA AGATCACAGC CTTAGATGAG 
TTTGCCACCA AGCTGATTCA GAACAACCAC TACGCGATGG AAGATGTGGC CACCCGGCGA 
GACGCACTGC TGAGCCGCCG CAACGCCCTC CATGAGAGAG CCATGTACCG CCGTGCCCAG 
CTTGCAGATT CCTTCCACTT GCAGCAGTTT TTCCGTGACT CTGATGAGCT CAAGAGTTGG 
GTCAACGAGA AGATGAAAAC AGCCACAGAT GAAGCTTATA AAGACCCATC CAACCTACAA 
GGGAAAGTGC AGAAGCACCA GGCCTTTGAG GCCGAGCTCT CAGCAAACCA GAGCCGCATC 
GACGCCTTGG AGAAAGCTGG GCAGAAGCTG ATCGACGTTG ACCACTATGC CAAGGATGAG 
GTGGCGACTC GGATGAATGA AGTTATCAGT CTGTGGAAGA AGCTGCTGGA GGCCACGGAG 
CTGAAAGGCA TAAAGCTCCG TGAGGCTAAC CAGCAGCAGC AGTTTAACCG CAACGTTGAG 
GACATCGAGC TGTGGCTGTA CGAAGTGGAA GGTCATCTGG CCTCGGATGA CTACGGCAAA 
GACCTCACCA ACGTCCAGAA CCTCCAGAAA AAGCATGCCC TGCTGGAGGC TGACGTGGCC 
GCTCACCAGG ATCGCATCGA TGGCATCACT ATCCAAGCCC GCCAGTTCCA GGATGCGGGC 
CACTTTGATG CTGAAAACAT CAAGAAGAAG CAGGAGGCCC TGGTAGCTCG CTACGAGGCA 
CTCAAGGAGC CCATGGTTGC CCGGAAGCAG AAGCTGGCCG ATTCCCTGCG GTTGCAGCAG 
CTCTTCCGTG ACGTTGAGGA TGAGGAGACG TGGATTCGGG AGAAAGAACC TATTGCCGCC 
TCCACCAACA GAGGCAAAGA TTTAATTGGA GTCCAGAACC TGCTAAAGAA ACACCAAGCG 
CTACAAGCAG AAATTGCTGG CCACGAGCCC CGCATCAAAG CAGTCACACA GAAGGGGAAC 
GCCATGGTAG AGGAGGGCCA TTTTGCTGCC GAGGACGTGA AGGCCAAGCT CCATGAACTG 
AACCAGAAGT GGGAGTCGCT GAAGTCCAAG GCCTCCCAGC GACGGCAGGA CCTGGAGGAC 
TCGCTGCAGG CCCAGCAGTA CTTCGCTGAT GCCAACGAGG CCGAGTCCTG GATGCGGGAG 
AAGGAGCCCA TCGTGGGCAG CACCGACTAT GGCAAAGATG AGGACTCTGC CGAGGCTCTG 
CTAAAGAAGC ACGAGGCATT GATGTCGGAT CTCAGCGCCT ATGGCAGCAG CATCCAGGCC 
CTGCGAGAGC AGGCCCAGTC GTGCCGGCAA CAAGTGGCCC CCATGGATGA CGAGACTGGG 
AAGGAGCTGG TCTTGGCGCT CTATGACTAT CAGGAGAAGA GTCCCCGAGA GGTCACCATG 
AAGAAAGGAG ACATTCTCAC CTTACTCAAC AGCACCAACA AGGACTGGTG GAAAGTGGAA 
GTGAACGACC GGCAGGGCTT CGTGCCGGCC GCCTACGTGA AGAAGCTGGA CCCAGCGCAG 
TCGGCCTCCC GGGAGAACCT CCTGGAAGAG CAGGGCAGCA TCGCGCTGCG GCAGGAGCAG 
ATTGACAACC AGACGCGCGT AACTAAGGAG GCCGGCAGCG TGTCTCTGCG TATGAAACAG 
GTGGAAGAAC TGTACCATTC TCTGCTGGAA CTGGGAGAGA AGCGTAAAGG CATGTTGGAG 
AAGAGCTGTA AGAAGTTCAT GCTGTTCCGT GAAGCGAACG AGCTGCAGCA GTGGATCAAC 
GAGAAGGAAG CGGCCCTGAC AAGCGAGGAG GTCGGCGCGG ACTTGGAGCA GGTCGAAGTG 
CTGCAGAAGA AGTTTGACGA CTTCCAGAAG GATCTCAAGG CCAATGAGTC ACGGCTGAAG 
GACATTAACA AGGTGGCTGA AGACCTGGAG TCGGAAGGCC TGATGGCGGA GGAGGTGCAG 
GCCGTGCAGC AGCAGGAGGT GTATGGCGCG ATGCCCAGGG ATGAATCTGA TTCCAAGACA 
GCCTCCCCGT GGAAGTCTGC TCGCCTGATG GTTCACACCG TGGCCACCTT TAATTCCATC 
AAGGAGCTCA ATGAGCGCTG GCGGTCCCTG CAGCAGCTGG CTGAGGAGCG GAGCCAACTC 
TTGGGCAGCG CCCACGAAGT GCAGCGGTTC CACAGAGATG CTGACGAAAC CAAAGAGTGG 
ATCGAGGAGA AGAACCAGGC TCTAAACACA GACAACTATG GCCACGACCT GGCCAGCGTC 
CAGGCCCTGC AGCGCAAGCA TGAGGGCTTC GAGAGGGACC TCGCGGCCCT TGGCGACAAG 
GTGAACTCGC TCGGGGAGAC AGCCGAGCGC CTGATCCAGT CCCACCCCGA GTCGGCAGAG 
GACCTGCAGG AGAAGTGCAC TGAGCTGAAC CAGGCATGGA GCAGCCTGGG CAAGCGTGCC 
GACCAGCGCA AGGCCAAGCT GGGCGACTCC CACGACCTGC AGCGCTTCCT CAGCGACTTC 
CGGGACCTCA TGTCTTGGAT CAATGGAATC CGAGGTCTGG TGTCCTCAGA CGAGCTAGCC 
AAGGATGTCA CTGGAGCTGA GGCCCTGCTG GAGCGACACC AGGAACACCG GACAGAAATT 
GATGCCAGAG CTGGCACTTT CCAGGCATTC GAGCAGTTTG GGCAGCAGCT GTTGGCTCAT 
GGGCACTACG CCAGCCCCGA GATCAAGGAG AAGCTCGATG TTCTAGACCA GGAGCGCGCA 
GACCTCGAGA AGGCCTGGGT CCAGCGCAGG ATGATGCTGG ATCAGTGCCT TGAATTGCAG 
CTGTTCCATC GGGATTGCGA GCAGGCCGAG AACTGGATGG CTGCCCGGGA GGCCTTCCTG 
AACACAGAAG ACAAAGGGGA CTCGCTAGAC AGCGTGGAGG CTCTGATCAA AAAACACGAG 
GACTTTGACA AAGCAATTAA TGTCCAGGAG GAGAAGATTG CTGCCCTGCA GTCCTTTGCT 
GACCAGCTCA TTGCTGGCGG TCACTACGCC AAAGGCGACA TCTCCAGCCG GCGCAATGAG 
GTTTTGGACA GGTGGCGCCG TCTAAAAGCT CAGATGATTG AGAAGAGGTC AAAGCTGGGA 
GAATCGCAGA CCCTCCAGCA GTTCAGCCGG GATGTAGACG AGATTGAAGC TTGGATCAGT 
GAAAAATTAC AGACTGCAAG TGATGAGTCA TATAAGGACC CCACCAACAT CCAGCTTTCC 
AAGCTGCTGA GCAAGCATCA GAAGCACCAG GCCTTCGAGG CAGAACTGCA CGCCAATGCG 
GACCGGATCC GCGGGGTCAT CGACGTGGGC AACTCCCTCA TTGACCGTGG GGCCTGTGCC 
GGCAGCGAGG ATGCCGTCAA GGCCCGGCTG GCCGCCTTAG CTGACCAGTG GCAGTTCCTG 
GTGCAGAAGT CGGCTGAGAA GAGCCAGAAG CTGAAAGAAG CCAACAAACA GCAGAACTTC 
AACACTGGCA TCAAGGACTT CGACTTCTGG CTCTCTGAGG TGGAAGCCCT GCTGGCCTCT 
GAGGATTATG GCAAAGACCT GGCTTCTGTG AACAACTTGC TAAAAAAACA TCAGCTGCTG 
GAGGCAGATA TCTCTGCCCA CGAGGACCGC CTGAAGGACC TCAACAGCCA GGCGGACAGC 
CTGATGACCA GCAGCGCCTT CGACACCTCC CAGGTGAAGG ATAAGCGGGA CACCATCAAC 
AGCCGTTTCC AGAAGATCAA GAGCATGGCC GCCTCCCGGC GAGCTAGGCT GAATGAGTCC 
CACCGCCTGC ACCAGTTCTT CCGGGACATG GACGATGAGG AGTCCTGGAT CAAGGAGAAG 
AAGCTGCTGG TGAGCTCAGA GGACTACGGC CGCGACCTGA CGGGCGTCCA GAACCTGAGG 
AAGAAGCACA AACGGCTGGA GGCAGAGCTG GCTGCCCACG AGCCGGCCAT TCAGGGTGTC 
CTGGACACTG GAAAGAAGCT GTCTGATGAC AACACCATCG GGAAGGAGGA GATTCAGCAG 
CGCCTGGCGC AGTTTGTGGA GCACTGGAAG GAGCTGAAGC AGCTGGCAGC TGCTCGGGGC 
CAGCGGCTAG AAGAATCTTT GGAATATCAG CAGTTTGTGG CCAACGTGGA GGAGGAGGAA 
GCCTGGATCA ATGAGAAGAT GACCCTGGTG GCCAGTGAGG ATTACGGAGA CACTCTTGCT 
GCCATCCAGG GCTTGCTGAA GAAACACGAA GCCTTCGAGA CGGACTTCAC TGTCCACAAG 
GACCGGGTGA ATGACGTCTG CACTAACGGA CAAGACCTGG TTAAGAAGAA CAATCACCAT 
GAGGAGAACA TCTCTTCCAA GATGAAGGGC TTGAACGGGA AAGTGTCAGA CTTGGAGAAG 
GCTGCCTCCC AGAGGAAGGC AAAGCTCGAC GAGAACTCGG CCTTCCTCCA GTTCAACTGG 
AAGGCCGATG TGGTGGAGTC CTGGATTGGT GAAAAGGAGA ACAGCTTGAA GACAGATGAT 
TATGGTCGAG ATCTGTCTTC TGTCCAAACT CTCCTCACCA AACAGGAAAC CTTCGATGCT 
GGCCTGCAGG CCTTCCAGCA GGAGGGCATC GCCAACATCA CTGCCCTCAA AGATCAGCTG 
CTGGCCGCCA AGCATGTTCA GTCCAAGGCC ATCGAGGCCC GCCACGCTGC CCTCATGAAG 
AGATGGAGCC AGCTCCTGGC CAATTCGGCC ACCCGGAAGA AGAAGCTGCT GGAGGCACAG 
AGTCACTTCC GCAAGGTGGA GGATCTCTTC CTGACCTTTG CCAAAAAGGC TTCAGCCTTC 
AACAGCTGGT TTGAAAATGC AGAGGAGGAC TTAACAGACC CAGTGCGCTG CAACTCCCTG 
GAAGAGATCA AGGCCTTGAG AGAGGCCCAC GATGCCTTCC GCTCCTCGCT GAGCTCAGCC 
CAGGCCGACT TCAACCAGCT GGCTGAGCTG GACCGCCAGA TCAAGAGCTT CCGCGTGGCC 
TCCAACCCCT ACACCTGGTT CACCATGGAG GCCCTGGAGG AGACCTGGAG GAACCTTCAG 
AAGATCATCA AGGAAAGGGA GCTGGAGCTG CAGAAGGAGC AGCGGCGGCA GGAGGAGAAC 
GACAAGCTGC GCCAGGAGTT CGCCCAGCAT GCCAACGCCT TCCACCAGTG GATCCAGGAG 
ACCAGAACGT ATCTCCTCGA TGGGTCCTGC ATGGTGGAAG AATCTGGGAC CCTTGAATCG 
CAGCTTGAAG CTACCAAACG CAAACACCAG GAGATTCGAG CCATGAGAAG TCAGCTCAAG 
AAGATTGAAG ACCTGGGGGC GGCCATGGAG GAAGCGCTGA TCCTGGACAA CAAGTACACG 
GAGCACAGCA CCGTGGGCCT GGCCCAGCAG TGGGACCAGC TCGACCAGCT GGGCATGCGG 
ATGCAGCACA ACCTGGAGCA GCAGATCCAG GCCAGGAACA CGACAGGAGT GACCGAGGAG 
GCCCTCAAAG AATTCAGCAT GATGTTCAAA CACTTCGACA AGGACAAGTC CGGCCGGCTG 
AATCACCAGG AGTTCAAATC CTGTCTGCGC TCCCTGGGCT ATGACCTGCC CATGGTGGAG 
GAAGGGGAGC CCGACCCTGA GTTCGAGGCC ATCTTGGATA CTGTGGATCC TAACAGGGAC 
GGCCACGTCT CCCTGCAAGA GTACATGGCC TTTATGATCA GCCGTGAGAC CGAGAATGTC 
AAGTCCAGCG AGGAGATTGA GAGCGCCTTC CGGGCCCTGA GCTCCGAAGG GAAGCCTTAT 
GTGACCAAGG AGGAGCTGTA CCAGAACCTG ACTCGGGAAC AAGCCGACTA CTGCGTCTCC 
CACATGAAGC CCTACGTGGA CGGCAAGGGC CGCGAACTCC CCACTGCTTT CGACTACGTT 
GAGTTCACCC GCTCGCTCTT CGTGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTAN1 ( XM_027966360.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_027822161.1 Ovis aries spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X7, mRNA.

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CloneID	OSm76654
Clone ID Related Accession (Same CDS sequence)	XM_027966360.1
Accession Version	XM_027966360.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7455bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-05
Organism	Ovis aries(sheep)
Product	spectrin alpha chain, non-erythrocytic 1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040254.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ovis aries Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTCAT CGTTTCGTAA ACGCAGAGTC CAGAAAATGG ACCCAAGCGG GGTCAAAGTG 
CTGGAGACAG CCGAGGACAT CCAGGAGAGG CGGCAGCAGG TCCTGGACAG ATACCACCGC 
TTCAAGGAGC TCTCGACCCT GAGGCGCCAG AAGCTTGAAG ACTCCTACCG ATTCCAGTTT 
TTCCAAAGAG ATGCTGAGGA GCTGGAGAAA TGGATTCAGG AGAAGCTTCA GATTGCCTCG 
GATGAGAATT ACAAAGACCC AACCAACCTG CAGGGCAAGC TGCAGAAGCA TCAAGCCTTC 
GAAGCCGAGG TGCAGGCCAA CTCGGGTGCC ATCGTGAAGC TTGATGAGAC CGGTAACCTG 
ATGATCTCAG AAGGGCACTT CGCGTCCGAA ACCATCCGGA CTCGTCTGAT GGAGCTGCAC 
CGCCAGTGGG AATTGCTTCT GGAGAAGATG CGGGAGAAAG GCATCAAGCT GCTGCAGGCC 
CAGAAGCTGG TGCAGTACCT GCGGGAGTGT GAGGACGTGA TGGACTGGAT CAATGACAAG 
GAAGCCATTG TGACATCTGA GGAGCTGGGC CAGGACCTGG AGCACGTGGA GGTGTTACAG 
AAGAAATTCG AAGAGTTTCA GACAGACATG GCTGCCCACG AAGAGAGAGT CAATGAAGTG 
AACCAGTTTG CTGCCAAGCT CATTCAGGAG CAGCACCCTG AGGAGGAGCT GATCAAGACA 
AAGGAGGACG AGGTGAATGC AGCCTGGCAG CGGCTGAAGG GCCTGGCCCT CCAGCGGCAG 
GGGAAGCTGT TCGGGGCAGC TGAGGTCCAA CGCTTTAACA GGGATGTGGA CGAGACCATT 
AGCTGGATTA AGGAGAAGGA ACAGTTAATG GCCTCTGATG ACTTTGGCCG GGACCTGGCC 
AGTGTCCAGG CTCTACTGCG GAAACACGAG GGTTTGGAGA GAGATCTTGC TGCCTTGGAG 
GACAAGGTCA AAGCCCTGTG TGCCGAGGCT GACCGCCTGC AGCAGTCCCA CCCTCTGAGT 
GCCACCCAGA TCCAAGTGAA GCGAGAGGAG CTGATCACCA ACTGGGAGCA GATCCGCACC 
CTGGCCGCGG AGAGACACGC ACGTCTCAAC GATTCCTACA GGCTTCAGCG CTTCCTTGCT 
GACTTCCGAG ACCTCACCAG CTGGGTCACT GAGATGAAAG CCCTCATCAA TGCAGATGAA 
CTTGCCAATG ATGTGGCCGG GGCCGAAGCC CTGCTGGATA GGCATCAGGA GCACAAGGGT 
GAAATTGACG CTCATGAAGA TAGCTTTAAA TCTGCAGATG AGTCTGGACA GGCCCTGCTT 
GCAGCTGGTC ACTATGCCTC AGATGAAGTG AGGGAGAAGC TGACCATCCT GTCCGAGGAG 
CGGGCCGCGC TACTAGAGCT CTGGGAGCTG CGACGGCAGC AATATGAGCA GTGCATGGAC 
CTGCAGCTCT TCTACCGCGA CACGGAGCAG GTGGACAACT GGATGAGCAA ACAGGAGGCG 
TTCCTGTTGA ACGAAGACCT GGGTGATTCC TTGGATAGTG TGGAAGCGCT TCTCAAGAAG 
CACGAAGACT TTGAGAAGTC CCTCAGCGCC CAGGAGGAGA AGATCACAGC CTTAGATGAG 
TTTGCCACCA AGCTGATTCA GAACAACCAC TACGCGATGG AAGATGTGGC CACCCGGCGA 
GACGCACTGC TGAGCCGCCG CAACGCCCTC CATGAGAGAG CCATGTACCG CCGTGCCCAG 
CTTGCAGATT CCTTCCACTT GCAGCAGTTT TTCCGTGACT CTGATGAGCT CAAGAGTTGG 
GTCAACGAGA AGATGAAAAC AGCCACAGAT GAAGCTTATA AAGACCCATC CAACCTACAA 
GGGAAAGTGC AGAAGCACCA GGCCTTTGAG GCCGAGCTCT CAGCAAACCA GAGCCGCATC 
GACGCCTTGG AGAAAGCTGG GCAGAAGCTG ATCGACGTTG ACCACTATGC CAAGGATGAG 
GTGGCGACTC GGATGAATGA AGTTATCAGT CTGTGGAAGA AGCTGCTGGA GGCCACGGAG 
CTGAAAGGCA TAAAGCTCCG TGAGGCTAAC CAGCAGCAGC AGTTTAACCG CAACGTTGAG 
GACATCGAGC TGTGGCTGTA CGAAGTGGAA GGTCATCTGG CCTCGGATGA CTACGGCAAA 
GACCTCACCA ACGTCCAGAA CCTCCAGAAA AAGCATGCCC TGCTGGAGGC TGACGTGGCC 
GCTCACCAGG ATCGCATCGA TGGCATCACT ATCCAAGCCC GCCAGTTCCA GGATGCGGGC 
CACTTTGATG CTGAAAACAT CAAGAAGAAG CAGGAGGCCC TGGTAGCTCG CTACGAGGCA 
CTCAAGGAGC CCATGGTTGC CCGGAAGCAG AAGCTGGCCG ATTCCCTGCG GTTGCAGCAG 
CTCTTCCGTG ACGTTGAGGA TGAGGAGACG TGGATTCGGG AGAAAGAACC TATTGCCGCC 
TCCACCAACA GAGGCAAAGA TTTAATTGGA GTCCAGAACC TGCTAAAGAA ACACCAAGCG 
CTACAAGCAG AAATTGCTGG CCACGAGCCC CGCATCAAAG CAGTCACACA GAAGGGGAAC 
GCCATGGTAG AGGAGGGCCA TTTTGCTGCC GAGGACGTGA AGGCCAAGCT CCATGAACTG 
AACCAGAAGT GGGAGTCGCT GAAGTCCAAG GCCTCCCAGC GACGGCAGGA CCTGGAGGAC 
TCGCTGCAGG CCCAGCAGTA CTTCGCTGAT GCCAACGAGG CCGAGTCCTG GATGCGGGAG 
AAGGAGCCCA TCGTGGGCAG CACCGACTAT GGCAAAGATG AGGACTCTGC CGAGGCTCTG 
CTAAAGAAGC ACGAGGCATT GATGTCGGAT CTCAGCGCCT ATGGCAGCAG CATCCAGGCC 
CTGCGAGAGC AGGCCCAGTC GTGCCGGCAA CAAGTGGCCC CCATGGATGA CGAGACTGGG 
AAGGAGCTGG TCTTGGCGCT CTATGACTAT CAGGAGAAGA GTCCCCGAGA GGTCACCATG 
AAGAAAGGAG ACATTCTCAC CTTACTCAAC AGCACCAACA AGGACTGGTG GAAAGTGGAA 
GTGAACGACC GGCAGGGCTT CGTGCCGGCC GCCTACGTGA AGAAGCTGGA CCCAGCGCAG 
TCGGCCTCCC GGGAGAACCT CCTGGAAGAG CAGGGCAGCA TCGCGCTGCG GCAGGAGCAG 
ATTGACAACC AGACGCGCGT AACTAAGGAG GCCGGCAGCG TGTCTCTGCG TATGAAACAG 
GTGGAAGAAC TGTACCATTC TCTGCTGGAA CTGGGAGAGA AGCGTAAAGG CATGTTGGAG 
AAGAGCTGTA AGAAGTTCAT GCTGTTCCGT GAAGCGAACG AGCTGCAGCA GTGGATCAAC 
GAGAAGGAAG CGGCCCTGAC AAGCGAGGAG GTCGGCGCGG ACTTGGAGCA GGTCGAAGTG 
CTGCAGAAGA AGTTTGACGA CTTCCAGAAG GATCTCAAGG CCAATGAGTC ACGGCTGAAG 
GACATTAACA AGGTGGCTGA AGACCTGGAG TCGGAAGGCC TGATGGCGGA GGAGGTGCAG 
GCCGTGCAGC AGCAGGAGGT GTATGGCGCG ATGCCCAGGG ATGAATCTGA TTCCAAGACA 
GCCTCCCCGT GGAAGTCTGC TCGCCTGATG GTTCACACCG TGGCCACCTT TAATTCCATC 
AAGGAGCTCA ATGAGCGCTG GCGGTCCCTG CAGCAGCTGG CTGAGGAGCG GAGCCAACTC 
TTGGGCAGCG CCCACGAAGT GCAGCGGTTC CACAGAGATG CTGACGAAAC CAAAGAGTGG 
ATCGAGGAGA AGAACCAGGC TCTAAACACA GACAACTATG GCCACGACCT GGCCAGCGTC 
CAGGCCCTGC AGCGCAAGCA TGAGGGCTTC GAGAGGGACC TCGCGGCCCT TGGCGACAAG 
GTGAACTCGC TCGGGGAGAC AGCCGAGCGC CTGATCCAGT CCCACCCCGA GTCGGCAGAG 
GACCTGCAGG AGAAGTGCAC TGAGCTGAAC CAGGCATGGA GCAGCCTGGG CAAGCGTGCC 
GACCAGCGCA AGGCCAAGCT GGGCGACTCC CACGACCTGC AGCGCTTCCT CAGCGACTTC 
CGGGACCTCA TGTCTTGGAT CAATGGAATC CGAGGTCTGG TGTCCTCAGA CGAGCTAGCC 
AAGGATGTCA CTGGAGCTGA GGCCCTGCTG GAGCGACACC AGGAACACCG GACAGAAATT 
GATGCCAGAG CTGGCACTTT CCAGGCATTC GAGCAGTTTG GGCAGCAGCT GTTGGCTCAT 
GGGCACTACG CCAGCCCCGA GATCAAGGAG AAGCTCGATG TTCTAGACCA GGAGCGCGCA 
GACCTCGAGA AGGCCTGGGT CCAGCGCAGG ATGATGCTGG ATCAGTGCCT TGAATTGCAG 
CTGTTCCATC GGGATTGCGA GCAGGCCGAG AACTGGATGG CTGCCCGGGA GGCCTTCCTG 
AACACAGAAG ACAAAGGGGA CTCGCTAGAC AGCGTGGAGG CTCTGATCAA AAAACACGAG 
GACTTTGACA AAGCAATTAA TGTCCAGGAG GAGAAGATTG CTGCCCTGCA GTCCTTTGCT 
GACCAGCTCA TTGCTGGCGG TCACTACGCC AAAGGCGACA TCTCCAGCCG GCGCAATGAG 
GTTTTGGACA GGTGGCGCCG TCTAAAAGCT CAGATGATTG AGAAGAGGTC AAAGCTGGGA 
GAATCGCAGA CCCTCCAGCA GTTCAGCCGG GATGTAGACG AGATTGAAGC TTGGATCAGT 
GAAAAATTAC AGACTGCAAG TGATGAGTCA TATAAGGACC CCACCAACAT CCAGAGCAAG 
CATCAGAAGC ACCAGGCCTT CGAGGCAGAA CTGCACGCCA ATGCGGACCG GATCCGCGGG 
GTCATCGACG TGGGCAACTC CCTCATTGAC CGTGGGGCCT GTGCCGGCAG CGAGGATGCC 
GTCAAGGCCC GGCTGGCCGC CTTAGCTGAC CAGTGGCAGT TCCTGGTGCA GAAGTCGGCT 
GAGAAGAGCC AGAAGCTGAA AGAAGCCAAC AAACAGCAGA ACTTCAACAC TGGCATCAAG 
GACTTCGACT TCTGGCTCTC TGAGGTGGAA GCCCTGCTGG CCTCTGAGGA TTATGGCAAA 
GACCTGGCTT CTGTGAACAA CTTGCTAAAA AAACATCAGC TGCTGGAGGC AGATATCTCT 
GCCCACGAGG ACCGCCTGAA GGACCTCAAC AGCCAGGCGG ACAGCCTGAT GACCAGCAGC 
GCCTTCGACA CCTCCCAGGT GAAGGATAAG CGGGACACCA TCAACAGCCG TTTCCAGAAG 
ATCAAGAGCA TGGCCGCCTC CCGGCGAGCT AGGCTGAATG AGTCCCACCG CCTGCACCAG 
TTCTTCCGGG ACATGGACGA TGAGGAGTCC TGGATCAAGG AGAAGAAGCT GCTGGTGAGC 
TCAGAGGACT ACGGCCGCGA CCTGACGGGC GTCCAGAACC TGAGGAAGAA GCACAAACGG 
CTGGAGGCAG AGCTGGCTGC CCACGAGCCG GCCATTCAGG GTGTCCTGGA CACTGGAAAG 
AAGCTGTCTG ATGACAACAC CATCGGGAAG GAGGAGATTC AGCAGCGCCT GGCGCAGTTT 
GTGGAGCACT GGAAGGAGCT GAAGCAGCTG GCAGCTGCTC GGGGCCAGCG GCTAGAAGAA 
TCTTTGGAAT ATCAGCAGTT TGTGGCCAAC GTGGAGGAGG AGGAAGCCTG GATCAATGAG 
AAGATGACCC TGGTGGCCAG TGAGGATTAC GGAGACACTC TTGCTGCCAT CCAGGGCTTG 
CTGAAGAAAC ACGAAGCCTT CGAGACGGAC TTCACTGTCC ACAAGGACCG GGTGAATGAC 
GTCTGCACTA ACGGACAAGA CCTGGTTAAG AAGAACAATC ACCATGAGGA GAACATCTCT 
TCCAAGATGA AGGGCTTGAA CGGGAAAGTG TCAGACTTGG AGAAGGCTGC CTCCCAGAGG 
AAGGCAAAGC TCGACGAGAA CTCGGCCTTC CTCCAGTTCA ACTGGAAGGC CGATGTGGTG 
GAGTCCTGGA TTGGTGAAAA GGAGAACAGC TTGAAGACAG ATGATTATGG TCGAGATCTG 
TCTTCTGTCC AAACTCTCCT CACCAAACAG GAAACCTTCG ATGCTGGCCT GCAGGCCTTC 
CAGCAGGAGG GCATCGCCAA CATCACTGCC CTCAAAGATC AGCTGCTGGC CGCCAAGCAT 
GTTCAGTCCA AGGCCATCGA GGCCCGCCAC GCTGCCCTCA TGAAGAGATG GAGCCAGCTC 
CTGGCCAATT CGGCCACCCG GAAGAAGAAG CTGCTGGAGG CACAGAGTCA CTTCCGCAAG 
GTGGAGGATC TCTTCCTGAC CTTTGCCAAA AAGGCTTCAG CCTTCAACAG CTGGTTTGAA 
AATGCAGAGG AGGACTTAAC AGACCCAGTG CGCTGCAACT CCCTGGAAGA GATCAAGGCC 
TTGAGAGAGG CCCACGATGC CTTCCGCTCC TCGCTGAGCT CAGCCCAGGC CGACTTCAAC 
CAGCTGGCTG AGCTGGACCG CCAGATCAAG AGCTTCCGCG TGGCCTCCAA CCCCTACACC 
TGGTTCACCA TGGAGGCCCT GGAGGAGACC TGGAGGAACC TTCAGAAGAT CATCAAGGAA 
AGGGAGCTGG AGCTGCAGAA GGAGCAGCGG CGGCAGGAGG AGAACGACAA GCTGCGCCAG 
GAGTTCGCCC AGCATGCCAA CGCCTTCCAC CAGTGGATCC AGGAGACCAG AACGTATCTC 
CTCGATGGGT CCTGCATGGT GGAAGAATCT GGGACCCTTG AATCGCAGCT TGAAGCTACC 
AAACGCAAAC ACCAGGAGAT TCGAGCCATG AGAAGTCAGC TCAAGAAGAT TGAAGACCTG 
GGGGCGGCCA TGGAGGAAGC GCTGATCCTG GACAACAAGT ACACGGAGCA CAGCACCGTG 
GGCCTGGCCC AGCAGTGGGA CCAGCTCGAC CAGCTGGGCA TGCGGATGCA GCACAACCTG 
GAGCAGCAGA TCCAGGCCAG GAACACGACA GGAGTGACCG AGGAGGCCCT CAAAGAATTC 
AGCATGATGT TCAAACACTT CGACAAGGAC AAGTCCGGCC GGCTGAATCA CCAGGAGTTC 
AAATCCTGTC TGCGCTCCCT GGGCTATGAC CTGCCCATGG TGGAGGAAGG GGAGCCCGAC 
CCTGAGTTCG AGGCCATCTT GGATACTGTG GATCCTAACA GGGACGGCCA CGTCTCCCTG 
CAAGAGTACA TGGCCTTTAT GATCAGCCGT GAGACCGAGA ATGTCAAGTC CAGCGAGGAG 
ATTGAGAGCG CCTTCCGGGC CCTGAGCTCC GAAGGGAAGC CTTATGTGAC CAAGGAGGAG 
CTGTACCAGA ACCTGACTCG GGAACAAGCC GACTACTGCG TCTCCCACAT GAAGCCCTAC 
GTGGACGGCA AGGGCCGCGA ACTCCCCACT GCTTTCGACT ACGTTGAGTT CACCCGCTCG 
CTCTTCGTGA ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_027822161.1
CDS	70..7524
Translation	MLSSFRKRRVQKMDPSGVKVLETAEDIQERRQQVLDRYHRFKELSTLRRQKLEDSYRFQFFQRDAEELEKWIQEKLQIASDENYKDPTNLQGKLQKHQAFEAEVQANSGAIVKLDETGNLMISEGHFASETIRTRLMELHRQWELLLEKMREKGIKLLQAQKLVQYLRECEDVMDWINDKEAIVTSEELGQDLEHVEVLQKKFEEFQTDMAAHEERVNEVNQFAAKLIQEQHPEEELIKTKEDEVNAAWQRLKGLALQRQGKLFGAAEVQRFNRDVDETISWIKEKEQLMASDDFGRDLASVQALLRKHEGLERDLAALEDKVKALCAEADRLQQSHPLSATQIQVKREELITNWEQIRTLAAERHARLNDSYRLQRFLADFRDLTSWVTEMKALINADELANDVAGAEALLDRHQEHKGEIDAHEDSFKSADESGQALLAAGHYASDEVREKLTILSEERAALLELWELRRQQYEQCMDLQLFYRDTEQVDNWMSKQEAFLLNEDLGDSLDSVEALLKKHEDFEKSLSAQEEKITALDEFATKLIQNNHYAMEDVATRRDALLSRRNALHERAMYRRAQLADSFHLQQFFRDSDELKSWVNEKMKTATDEAYKDPSNLQGKVQKHQAFEAELSANQSRIDALEKAGQKLIDVDHYAKDEVATRMNEVISLWKKLLEATELKGIKLREANQQQQFNRNVEDIELWLYEVEGHLASDDYGKDLTNVQNLQKKHALLEADVAAHQDRIDGITIQARQFQDAGHFDAENIKKKQEALVARYEALKEPMVARKQKLADSLRLQQLFRDVEDEETWIREKEPIAASTNRGKDLIGVQNLLKKHQALQAEIAGHEPRIKAVTQKGNAMVEEGHFAAEDVKAKLHELNQKWESLKSKASQRRQDLEDSLQAQQYFADANEAESWMREKEPIVGSTDYGKDEDSAEALLKKHEALMSDLSAYGSSIQALREQAQSCRQQVAPMDDETGKELVLALYDYQEKSPREVTMKKGDILTLLNSTNKDWWKVEVNDRQGFVPAAYVKKLDPAQSASRENLLEEQGSIALRQEQIDNQTRVTKEAGSVSLRMKQVEELYHSLLELGEKRKGMLEKSCKKFMLFREANELQQWINEKEAALTSEEVGADLEQVEVLQKKFDDFQKDLKANESRLKDINKVAEDLESEGLMAEEVQAVQQQEVYGAMPRDESDSKTASPWKSARLMVHTVATFNSIKELNERWRSLQQLAEERSQLLGSAHEVQRFHRDADETKEWIEEKNQALNTDNYGHDLASVQALQRKHEGFERDLAALGDKVNSLGETAERLIQSHPESAEDLQEKCTELNQAWSSLGKRADQRKAKLGDSHDLQRFLSDFRDLMSWINGIRGLVSSDELAKDVTGAEALLERHQEHRTEIDARAGTFQAFEQFGQQLLAHGHYASPEIKEKLDVLDQERADLEKAWVQRRMMLDQCLELQLFHRDCEQAENWMAAREAFLNTEDKGDSLDSVEALIKKHEDFDKAINVQEEKIAALQSFADQLIAGGHYAKGDISSRRNEVLDRWRRLKAQMIEKRSKLGESQTLQQFSRDVDEIEAWISEKLQTASDESYKDPTNIQSKHQKHQAFEAELHANADRIRGVIDVGNSLIDRGACAGSEDAVKARLAALADQWQFLVQKSAEKSQKLKEANKQQNFNTGIKDFDFWLSEVEALLASEDYGKDLASVNNLLKKHQLLEADISAHEDRLKDLNSQADSLMTSSAFDTSQVKDKRDTINSRFQKIKSMAASRRARLNESHRLHQFFRDMDDEESWIKEKKLLVSSEDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQFVEHWKELKQLAAARGQRLEESLEYQQFVANVEEEEAWINEKMTLVASEDYGDTLAAIQGLLKKHEAFETDFTVHKDRVNDVCTNGQDLVKKNNHHEENISSKMKGLNGKVSDLEKAASQRKAKLDENSAFLQFNWKADVVESWIGEKENSLKTDDYGRDLSSVQTLLTKQETFDAGLQAFQQEGIANITALKDQLLAAKHVQSKAIEARHAALMKRWSQLLANSATRKKKLLEAQSHFRKVEDLFLTFAKKASAFNSWFENAEEDLTDPVRCNSLEEIKALREAHDAFRSSLSSAQADFNQLAELDRQIKSFRVASNPYTWFTMEALEETWRNLQKIIKERELELQKEQRRQEENDKLRQEFAQHANAFHQWIQETRTYLLDGSCMVEESGTLESQLEATKRKHQEIRAMRSQLKKIEDLGAAMEEALILDNKYTEHSTVGLAQQWDQLDQLGMRMQHNLEQQIQARNTTGVTEEALKEFSMMFKHFDKDKSGRLNHQEFKSCLRSLGYDLPMVEEGEPDPEFEAILDTVDPNRDGHVSLQEYMAFMISRETENVKSSEEIESAFRALSSEGKPYVTKEELYQNLTREQADYCVSHMKPYVDGKGRELPTAFDYVEFTRSLFVN

Target ORF information:

RefSeq Version	XM_027966360.1
Organism	Ovis aries(sheep)
Definition	Ovis aries spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027966360.1

ORF Insert Sequence:

ATGCTGTCAT CGTTTCGTAA ACGCAGAGTC CAGAAAATGG ACCCAAGCGG GGTCAAAGTG 
CTGGAGACAG CCGAGGACAT CCAGGAGAGG CGGCAGCAGG TCCTGGACAG ATACCACCGC 
TTCAAGGAGC TCTCGACCCT GAGGCGCCAG AAGCTTGAAG ACTCCTACCG ATTCCAGTTT 
TTCCAAAGAG ATGCTGAGGA GCTGGAGAAA TGGATTCAGG AGAAGCTTCA GATTGCCTCG 
GATGAGAATT ACAAAGACCC AACCAACCTG CAGGGCAAGC TGCAGAAGCA TCAAGCCTTC 
GAAGCCGAGG TGCAGGCCAA CTCGGGTGCC ATCGTGAAGC TTGATGAGAC CGGTAACCTG 
ATGATCTCAG AAGGGCACTT CGCGTCCGAA ACCATCCGGA CTCGTCTGAT GGAGCTGCAC 
CGCCAGTGGG AATTGCTTCT GGAGAAGATG CGGGAGAAAG GCATCAAGCT GCTGCAGGCC 
CAGAAGCTGG TGCAGTACCT GCGGGAGTGT GAGGACGTGA TGGACTGGAT CAATGACAAG 
GAAGCCATTG TGACATCTGA GGAGCTGGGC CAGGACCTGG AGCACGTGGA GGTGTTACAG 
AAGAAATTCG AAGAGTTTCA GACAGACATG GCTGCCCACG AAGAGAGAGT CAATGAAGTG 
AACCAGTTTG CTGCCAAGCT CATTCAGGAG CAGCACCCTG AGGAGGAGCT GATCAAGACA 
AAGGAGGACG AGGTGAATGC AGCCTGGCAG CGGCTGAAGG GCCTGGCCCT CCAGCGGCAG 
GGGAAGCTGT TCGGGGCAGC TGAGGTCCAA CGCTTTAACA GGGATGTGGA CGAGACCATT 
AGCTGGATTA AGGAGAAGGA ACAGTTAATG GCCTCTGATG ACTTTGGCCG GGACCTGGCC 
AGTGTCCAGG CTCTACTGCG GAAACACGAG GGTTTGGAGA GAGATCTTGC TGCCTTGGAG 
GACAAGGTCA AAGCCCTGTG TGCCGAGGCT GACCGCCTGC AGCAGTCCCA CCCTCTGAGT 
GCCACCCAGA TCCAAGTGAA GCGAGAGGAG CTGATCACCA ACTGGGAGCA GATCCGCACC 
CTGGCCGCGG AGAGACACGC ACGTCTCAAC GATTCCTACA GGCTTCAGCG CTTCCTTGCT 
GACTTCCGAG ACCTCACCAG CTGGGTCACT GAGATGAAAG CCCTCATCAA TGCAGATGAA 
CTTGCCAATG ATGTGGCCGG GGCCGAAGCC CTGCTGGATA GGCATCAGGA GCACAAGGGT 
GAAATTGACG CTCATGAAGA TAGCTTTAAA TCTGCAGATG AGTCTGGACA GGCCCTGCTT 
GCAGCTGGTC ACTATGCCTC AGATGAAGTG AGGGAGAAGC TGACCATCCT GTCCGAGGAG 
CGGGCCGCGC TACTAGAGCT CTGGGAGCTG CGACGGCAGC AATATGAGCA GTGCATGGAC 
CTGCAGCTCT TCTACCGCGA CACGGAGCAG GTGGACAACT GGATGAGCAA ACAGGAGGCG 
TTCCTGTTGA ACGAAGACCT GGGTGATTCC TTGGATAGTG TGGAAGCGCT TCTCAAGAAG 
CACGAAGACT TTGAGAAGTC CCTCAGCGCC CAGGAGGAGA AGATCACAGC CTTAGATGAG 
TTTGCCACCA AGCTGATTCA GAACAACCAC TACGCGATGG AAGATGTGGC CACCCGGCGA 
GACGCACTGC TGAGCCGCCG CAACGCCCTC CATGAGAGAG CCATGTACCG CCGTGCCCAG 
CTTGCAGATT CCTTCCACTT GCAGCAGTTT TTCCGTGACT CTGATGAGCT CAAGAGTTGG 
GTCAACGAGA AGATGAAAAC AGCCACAGAT GAAGCTTATA AAGACCCATC CAACCTACAA 
GGGAAAGTGC AGAAGCACCA GGCCTTTGAG GCCGAGCTCT CAGCAAACCA GAGCCGCATC 
GACGCCTTGG AGAAAGCTGG GCAGAAGCTG ATCGACGTTG ACCACTATGC CAAGGATGAG 
GTGGCGACTC GGATGAATGA AGTTATCAGT CTGTGGAAGA AGCTGCTGGA GGCCACGGAG 
CTGAAAGGCA TAAAGCTCCG TGAGGCTAAC CAGCAGCAGC AGTTTAACCG CAACGTTGAG 
GACATCGAGC TGTGGCTGTA CGAAGTGGAA GGTCATCTGG CCTCGGATGA CTACGGCAAA 
GACCTCACCA ACGTCCAGAA CCTCCAGAAA AAGCATGCCC TGCTGGAGGC TGACGTGGCC 
GCTCACCAGG ATCGCATCGA TGGCATCACT ATCCAAGCCC GCCAGTTCCA GGATGCGGGC 
CACTTTGATG CTGAAAACAT CAAGAAGAAG CAGGAGGCCC TGGTAGCTCG CTACGAGGCA 
CTCAAGGAGC CCATGGTTGC CCGGAAGCAG AAGCTGGCCG ATTCCCTGCG GTTGCAGCAG 
CTCTTCCGTG ACGTTGAGGA TGAGGAGACG TGGATTCGGG AGAAAGAACC TATTGCCGCC 
TCCACCAACA GAGGCAAAGA TTTAATTGGA GTCCAGAACC TGCTAAAGAA ACACCAAGCG 
CTACAAGCAG AAATTGCTGG CCACGAGCCC CGCATCAAAG CAGTCACACA GAAGGGGAAC 
GCCATGGTAG AGGAGGGCCA TTTTGCTGCC GAGGACGTGA AGGCCAAGCT CCATGAACTG 
AACCAGAAGT GGGAGTCGCT GAAGTCCAAG GCCTCCCAGC GACGGCAGGA CCTGGAGGAC 
TCGCTGCAGG CCCAGCAGTA CTTCGCTGAT GCCAACGAGG CCGAGTCCTG GATGCGGGAG 
AAGGAGCCCA TCGTGGGCAG CACCGACTAT GGCAAAGATG AGGACTCTGC CGAGGCTCTG 
CTAAAGAAGC ACGAGGCATT GATGTCGGAT CTCAGCGCCT ATGGCAGCAG CATCCAGGCC 
CTGCGAGAGC AGGCCCAGTC GTGCCGGCAA CAAGTGGCCC CCATGGATGA CGAGACTGGG 
AAGGAGCTGG TCTTGGCGCT CTATGACTAT CAGGAGAAGA GTCCCCGAGA GGTCACCATG 
AAGAAAGGAG ACATTCTCAC CTTACTCAAC AGCACCAACA AGGACTGGTG GAAAGTGGAA 
GTGAACGACC GGCAGGGCTT CGTGCCGGCC GCCTACGTGA AGAAGCTGGA CCCAGCGCAG 
TCGGCCTCCC GGGAGAACCT CCTGGAAGAG CAGGGCAGCA TCGCGCTGCG GCAGGAGCAG 
ATTGACAACC AGACGCGCGT AACTAAGGAG GCCGGCAGCG TGTCTCTGCG TATGAAACAG 
GTGGAAGAAC TGTACCATTC TCTGCTGGAA CTGGGAGAGA AGCGTAAAGG CATGTTGGAG 
AAGAGCTGTA AGAAGTTCAT GCTGTTCCGT GAAGCGAACG AGCTGCAGCA GTGGATCAAC 
GAGAAGGAAG CGGCCCTGAC AAGCGAGGAG GTCGGCGCGG ACTTGGAGCA GGTCGAAGTG 
CTGCAGAAGA AGTTTGACGA CTTCCAGAAG GATCTCAAGG CCAATGAGTC ACGGCTGAAG 
GACATTAACA AGGTGGCTGA AGACCTGGAG TCGGAAGGCC TGATGGCGGA GGAGGTGCAG 
GCCGTGCAGC AGCAGGAGGT GTATGGCGCG ATGCCCAGGG ATGAATCTGA TTCCAAGACA 
GCCTCCCCGT GGAAGTCTGC TCGCCTGATG GTTCACACCG TGGCCACCTT TAATTCCATC 
AAGGAGCTCA ATGAGCGCTG GCGGTCCCTG CAGCAGCTGG CTGAGGAGCG GAGCCAACTC 
TTGGGCAGCG CCCACGAAGT GCAGCGGTTC CACAGAGATG CTGACGAAAC CAAAGAGTGG 
ATCGAGGAGA AGAACCAGGC TCTAAACACA GACAACTATG GCCACGACCT GGCCAGCGTC 
CAGGCCCTGC AGCGCAAGCA TGAGGGCTTC GAGAGGGACC TCGCGGCCCT TGGCGACAAG 
GTGAACTCGC TCGGGGAGAC AGCCGAGCGC CTGATCCAGT CCCACCCCGA GTCGGCAGAG 
GACCTGCAGG AGAAGTGCAC TGAGCTGAAC CAGGCATGGA GCAGCCTGGG CAAGCGTGCC 
GACCAGCGCA AGGCCAAGCT GGGCGACTCC CACGACCTGC AGCGCTTCCT CAGCGACTTC 
CGGGACCTCA TGTCTTGGAT CAATGGAATC CGAGGTCTGG TGTCCTCAGA CGAGCTAGCC 
AAGGATGTCA CTGGAGCTGA GGCCCTGCTG GAGCGACACC AGGAACACCG GACAGAAATT 
GATGCCAGAG CTGGCACTTT CCAGGCATTC GAGCAGTTTG GGCAGCAGCT GTTGGCTCAT 
GGGCACTACG CCAGCCCCGA GATCAAGGAG AAGCTCGATG TTCTAGACCA GGAGCGCGCA 
GACCTCGAGA AGGCCTGGGT CCAGCGCAGG ATGATGCTGG ATCAGTGCCT TGAATTGCAG 
CTGTTCCATC GGGATTGCGA GCAGGCCGAG AACTGGATGG CTGCCCGGGA GGCCTTCCTG 
AACACAGAAG ACAAAGGGGA CTCGCTAGAC AGCGTGGAGG CTCTGATCAA AAAACACGAG 
GACTTTGACA AAGCAATTAA TGTCCAGGAG GAGAAGATTG CTGCCCTGCA GTCCTTTGCT 
GACCAGCTCA TTGCTGGCGG TCACTACGCC AAAGGCGACA TCTCCAGCCG GCGCAATGAG 
GTTTTGGACA GGTGGCGCCG TCTAAAAGCT CAGATGATTG AGAAGAGGTC AAAGCTGGGA 
GAATCGCAGA CCCTCCAGCA GTTCAGCCGG GATGTAGACG AGATTGAAGC TTGGATCAGT 
GAAAAATTAC AGACTGCAAG TGATGAGTCA TATAAGGACC CCACCAACAT CCAGAGCAAG 
CATCAGAAGC ACCAGGCCTT CGAGGCAGAA CTGCACGCCA ATGCGGACCG GATCCGCGGG 
GTCATCGACG TGGGCAACTC CCTCATTGAC CGTGGGGCCT GTGCCGGCAG CGAGGATGCC 
GTCAAGGCCC GGCTGGCCGC CTTAGCTGAC CAGTGGCAGT TCCTGGTGCA GAAGTCGGCT 
GAGAAGAGCC AGAAGCTGAA AGAAGCCAAC AAACAGCAGA ACTTCAACAC TGGCATCAAG 
GACTTCGACT TCTGGCTCTC TGAGGTGGAA GCCCTGCTGG CCTCTGAGGA TTATGGCAAA 
GACCTGGCTT CTGTGAACAA CTTGCTAAAA AAACATCAGC TGCTGGAGGC AGATATCTCT 
GCCCACGAGG ACCGCCTGAA GGACCTCAAC AGCCAGGCGG ACAGCCTGAT GACCAGCAGC 
GCCTTCGACA CCTCCCAGGT GAAGGATAAG CGGGACACCA TCAACAGCCG TTTCCAGAAG 
ATCAAGAGCA TGGCCGCCTC CCGGCGAGCT AGGCTGAATG AGTCCCACCG CCTGCACCAG 
TTCTTCCGGG ACATGGACGA TGAGGAGTCC TGGATCAAGG AGAAGAAGCT GCTGGTGAGC 
TCAGAGGACT ACGGCCGCGA CCTGACGGGC GTCCAGAACC TGAGGAAGAA GCACAAACGG 
CTGGAGGCAG AGCTGGCTGC CCACGAGCCG GCCATTCAGG GTGTCCTGGA CACTGGAAAG 
AAGCTGTCTG ATGACAACAC CATCGGGAAG GAGGAGATTC AGCAGCGCCT GGCGCAGTTT 
GTGGAGCACT GGAAGGAGCT GAAGCAGCTG GCAGCTGCTC GGGGCCAGCG GCTAGAAGAA 
TCTTTGGAAT ATCAGCAGTT TGTGGCCAAC GTGGAGGAGG AGGAAGCCTG GATCAATGAG 
AAGATGACCC TGGTGGCCAG TGAGGATTAC GGAGACACTC TTGCTGCCAT CCAGGGCTTG 
CTGAAGAAAC ACGAAGCCTT CGAGACGGAC TTCACTGTCC ACAAGGACCG GGTGAATGAC 
GTCTGCACTA ACGGACAAGA CCTGGTTAAG AAGAACAATC ACCATGAGGA GAACATCTCT 
TCCAAGATGA AGGGCTTGAA CGGGAAAGTG TCAGACTTGG AGAAGGCTGC CTCCCAGAGG 
AAGGCAAAGC TCGACGAGAA CTCGGCCTTC CTCCAGTTCA ACTGGAAGGC CGATGTGGTG 
GAGTCCTGGA TTGGTGAAAA GGAGAACAGC TTGAAGACAG ATGATTATGG TCGAGATCTG 
TCTTCTGTCC AAACTCTCCT CACCAAACAG GAAACCTTCG ATGCTGGCCT GCAGGCCTTC 
CAGCAGGAGG GCATCGCCAA CATCACTGCC CTCAAAGATC AGCTGCTGGC CGCCAAGCAT 
GTTCAGTCCA AGGCCATCGA GGCCCGCCAC GCTGCCCTCA TGAAGAGATG GAGCCAGCTC 
CTGGCCAATT CGGCCACCCG GAAGAAGAAG CTGCTGGAGG CACAGAGTCA CTTCCGCAAG 
GTGGAGGATC TCTTCCTGAC CTTTGCCAAA AAGGCTTCAG CCTTCAACAG CTGGTTTGAA 
AATGCAGAGG AGGACTTAAC AGACCCAGTG CGCTGCAACT CCCTGGAAGA GATCAAGGCC 
TTGAGAGAGG CCCACGATGC CTTCCGCTCC TCGCTGAGCT CAGCCCAGGC CGACTTCAAC 
CAGCTGGCTG AGCTGGACCG CCAGATCAAG AGCTTCCGCG TGGCCTCCAA CCCCTACACC 
TGGTTCACCA TGGAGGCCCT GGAGGAGACC TGGAGGAACC TTCAGAAGAT CATCAAGGAA 
AGGGAGCTGG AGCTGCAGAA GGAGCAGCGG CGGCAGGAGG AGAACGACAA GCTGCGCCAG 
GAGTTCGCCC AGCATGCCAA CGCCTTCCAC CAGTGGATCC AGGAGACCAG AACGTATCTC 
CTCGATGGGT CCTGCATGGT GGAAGAATCT GGGACCCTTG AATCGCAGCT TGAAGCTACC 
AAACGCAAAC ACCAGGAGAT TCGAGCCATG AGAAGTCAGC TCAAGAAGAT TGAAGACCTG 
GGGGCGGCCA TGGAGGAAGC GCTGATCCTG GACAACAAGT ACACGGAGCA CAGCACCGTG 
GGCCTGGCCC AGCAGTGGGA CCAGCTCGAC CAGCTGGGCA TGCGGATGCA GCACAACCTG 
GAGCAGCAGA TCCAGGCCAG GAACACGACA GGAGTGACCG AGGAGGCCCT CAAAGAATTC 
AGCATGATGT TCAAACACTT CGACAAGGAC AAGTCCGGCC GGCTGAATCA CCAGGAGTTC 
AAATCCTGTC TGCGCTCCCT GGGCTATGAC CTGCCCATGG TGGAGGAAGG GGAGCCCGAC 
CCTGAGTTCG AGGCCATCTT GGATACTGTG GATCCTAACA GGGACGGCCA CGTCTCCCTG 
CAAGAGTACA TGGCCTTTAT GATCAGCCGT GAGACCGAGA ATGTCAAGTC CAGCGAGGAG 
ATTGAGAGCG CCTTCCGGGC CCTGAGCTCC GAAGGGAAGC CTTATGTGAC CAAGGAGGAG 
CTGTACCAGA ACCTGACTCG GGAACAAGCC GACTACTGCG TCTCCCACAT GAAGCCCTAC 
GTGGACGGCA AGGGCCGCGA ACTCCCCACT GCTTTCGACT ACGTTGAGTT CACCCGCTCG 
CTCTTCGTGA ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTAN1 ( XM_027966361.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_027822162.1 Ovis aries spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X8, mRNA.

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CloneID	OSm76655
Clone ID Related Accession (Same CDS sequence)	XM_027966361.1
Accession Version	XM_027966361.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7452bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-05
Organism	Ovis aries(sheep)
Product	spectrin alpha chain, non-erythrocytic 1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040254.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ovis aries Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTCAT CGTTTCGTAA ACGCAGAGTC CAGAAAATGG ACCCAAGCGG GGTCAAAGTG 
CTGGAGACAG CCGAGGACAT CCAGGAGAGG CGGCAGCAGG TCCTGGACAG ATACCACCGC 
TTCAAGGAGC TCTCGACCCT GAGGCGCCAG AAGCTTGAAG ACTCCTACCG ATTCCAGTTT 
TTCCAAAGAG ATGCTGAGGA GCTGGAGAAA TGGATTCAGG AGAAGCTTCA GATTGCCTCG 
GATGAGAATT ACAAAGACCC AACCAACCTG CAGGGCAAGC TGCAGAAGCA TCAAGCCTTC 
GAAGCCGAGG TGCAGGCCAA CTCGGGTGCC ATCGTGAAGC TTGATGAGAC CGGTAACCTG 
ATGATCTCAG AAGGGCACTT CGCGTCCGAA ACCATCCGGA CTCGTCTGAT GGAGCTGCAC 
CGCCAGTGGG AATTGCTTCT GGAGAAGATG CGGGAGAAAG GCATCAAGCT GCTGCAGGCC 
CAGAAGCTGG TGCAGTACCT GCGGGAGTGT GAGGACGTGA TGGACTGGAT CAATGACAAG 
GAAGCCATTG TGACATCTGA GGAGCTGGGC CAGGACCTGG AGCACGTGGA GGTGTTACAG 
AAGAAATTCG AAGAGTTTCA GACAGACATG GCTGCCCACG AAGAGAGAGT CAATGAAGTG 
AACCAGTTTG CTGCCAAGCT CATTCAGGAG CAGCACCCTG AGGAGGAGCT GATCAAGACA 
AAGGAGGACG AGGTGAATGC AGCCTGGCAG CGGCTGAAGG GCCTGGCCCT CCAGCGGCAG 
GGGAAGCTGT TCGGGGCAGC TGAGGTCCAA CGCTTTAACA GGGATGTGGA CGAGACCATT 
AGCTGGATTA AGGAGAAGGA ACAGTTAATG GCCTCTGATG ACTTTGGCCG GGACCTGGCC 
AGTGTCCAGG CTCTACTGCG GAAACACGAG GGTTTGGAGA GAGATCTTGC TGCCTTGGAG 
GACAAGGTCA AAGCCCTGTG TGCCGAGGCT GACCGCCTGC AGCAGTCCCA CCCTCTGAGT 
GCCACCCAGA TCCAAGTGAA GCGAGAGGAG CTGATCACCA ACTGGGAGCA GATCCGCACC 
CTGGCCGCGG AGAGACACGC ACGTCTCAAC GATTCCTACA GGCTTCAGCG CTTCCTTGCT 
GACTTCCGAG ACCTCACCAG CTGGGTCACT GAGATGAAAG CCCTCATCAA TGCAGATGAA 
CTTGCCAATG ATGTGGCCGG GGCCGAAGCC CTGCTGGATA GGCATCAGGA GCACAAGGGT 
GAAATTGACG CTCATGAAGA TAGCTTTAAA TCTGCAGATG AGTCTGGACA GGCCCTGCTT 
GCAGCTGGTC ACTATGCCTC AGATGAAGTG AGGGAGAAGC TGACCATCCT GTCCGAGGAG 
CGGGCCGCGC TACTAGAGCT CTGGGAGCTG CGACGGCAGC AATATGAGCA GTGCATGGAC 
CTGCAGCTCT TCTACCGCGA CACGGAGCAG GTGGACAACT GGATGAGCAA ACAGGAGGCG 
TTCCTGTTGA ACGAAGACCT GGGTGATTCC TTGGATAGTG TGGAAGCGCT TCTCAAGAAG 
CACGAAGACT TTGAGAAGTC CCTCAGCGCC CAGGAGGAGA AGATCACAGC CTTAGATGAG 
TTTGCCACCA AGCTGATTCA GAACAACCAC TACGCGATGG AAGATGTGGC CACCCGGCGA 
GACGCACTGC TGAGCCGCCG CAACGCCCTC CATGAGAGAG CCATGTACCG CCGTGCCCAG 
CTTGCAGATT CCTTCCACTT GCAGCAGTTT TTCCGTGACT CTGATGAGCT CAAGAGTTGG 
GTCAACGAGA AGATGAAAAC AGCCACAGAT GAAGCTTATA AAGACCCATC CAACCTACAA 
GGGAAAGTGC AGAAGCACCA GGCCTTTGAG GCCGAGCTCT CAGCAAACCA GAGCCGCATC 
GACGCCTTGG AGAAAGCTGG GCAGAAGCTG ATCGACGTTG ACCACTATGC CAAGGATGAG 
GTGGCGACTC GGATGAATGA AGTTATCAGT CTGTGGAAGA AGCTGCTGGA GGCCACGGAG 
CTGAAAGGCA TAAAGCTCCG TGAGGCTAAC CAGCAGCAGC AGTTTAACCG CAACGTTGAG 
GACATCGAGC TGTGGCTGTA CGAAGTGGAA GGTCATCTGG CCTCGGATGA CTACGGCAAA 
GACCTCACCA ACGTCCAGAA CCTCCAGAAA AAGCATGCCC TGCTGGAGGC TGACGTGGCC 
GCTCACCAGG ATCGCATCGA TGGCATCACT ATCCAAGCCC GCCAGTTCCA GGATGCGGGC 
CACTTTGATG CTGAAAACAT CAAGAAGAAG CAGGAGGCCC TGGTAGCTCG CTACGAGGCA 
CTCAAGGAGC CCATGGTTGC CCGGAAGCAG AAGCTGGCCG ATTCCCTGCG GTTGCAGCAG 
CTCTTCCGTG ACGTTGAGGA TGAGGAGACG TGGATTCGGG AGAAAGAACC TATTGCCGCC 
TCCACCAACA GAGGCAAAGA TTTAATTGGA GTCCAGAACC TGCTAAAGAA ACACCAAGCG 
CTACAAGCAG AAATTGCTGG CCACGAGCCC CGCATCAAAG CAGTCACACA GAAGGGGAAC 
GCCATGGTAG AGGAGGGCCA TTTTGCTGCC GAGGACGTGA AGGCCAAGCT CCATGAACTG 
AACCAGAAGT GGGAGTCGCT GAAGTCCAAG GCCTCCCAGC GACGGCAGGA CCTGGAGGAC 
TCGCTGCAGG CCCAGCAGTA CTTCGCTGAT GCCAACGAGG CCGAGTCCTG GATGCGGGAG 
AAGGAGCCCA TCGTGGGCAG CACCGACTAT GGCAAAGATG AGGACTCTGC CGAGGCTCTG 
CTAAAGAAGC ACGAGGCATT GATGTCGGAT CTCAGCGCCT ATGGCAGCAG CATCCAGGCC 
CTGCGAGAGC AGGCCCAGTC GTGCCGGCAA CAAGTGGCCC CCATGGATGA CGAGACTGGG 
AAGGAGCTGG TCTTGGCGCT CTATGACTAT CAGGAGAAGA GTCCCCGAGA GGTCACCATG 
AAGAAAGGAG ACATTCTCAC CTTACTCAAC AGCACCAACA AGGACTGGTG GAAAGTGGAA 
GTGAACGACC GGCAGGGCTT CGTGCCGGCC GCCTACGTGA AGAAGCTGGA CCCAGCGCAG 
TCGGCCTCCC GGGAGAACCT CCTGGAAGAG CAGGGCAGCA TCGCGCTGCG GCAGGAGCAG 
ATTGACAACC AGACGCGCGT AACTAAGGAG GCCGGCAGCG TGTCTCTGCG TATGAAACAG 
GTGGAAGAAC TGTACCATTC TCTGCTGGAA CTGGGAGAGA AGCGTAAAGG CATGTTGGAG 
AAGAGCTGTA AGAAGTTCAT GCTGTTCCGT GAAGCGAACG AGCTGCAGCA GTGGATCAAC 
GAGAAGGAAG CGGCCCTGAC AAGCGAGGAG GTCGGCGCGG ACTTGGAGCA GGTCGAAGTG 
CTGCAGAAGA AGTTTGACGA CTTCCAGAAG GATCTCAAGG CCAATGAGTC ACGGCTGAAG 
GACATTAACA AGGTGGCTGA AGACCTGGAG TCGGAAGGCC TGATGGCGGA GGAGGTGCAG 
GCCGTGCAGC AGCAGGAGGT GTATGGCGCG ATGCCCAGGG ATGAATCTGA TTCCAAGACA 
GCCTCCCCGT GGAAGTCTGC TCGCCTGATG GTTCACACCG TGGCCACCTT TAATTCCATC 
AAGGAGCTCA ATGAGCGCTG GCGGTCCCTG CAGCAGCTGG CTGAGGAGCG GAGCCAACTC 
TTGGGCAGCG CCCACGAAGT GCAGCGGTTC CACAGAGATG CTGACGAAAC CAAAGAGTGG 
ATCGAGGAGA AGAACCAGGC TCTAAACACA GACAACTATG GCCACGACCT GGCCAGCGTC 
CAGGCCCTGC AGCGCAAGCA TGAGGGCTTC GAGAGGGACC TCGCGGCCCT TGGCGACAAG 
GTGAACTCGC TCGGGGAGAC AGCCGAGCGC CTGATCCAGT CCCACCCCGA GTCGGCAGAG 
GACCTGCAGG AGAAGTGCAC TGAGCTGAAC CAGGCATGGA GCAGCCTGGG CAAGCGTGCC 
GACCAGCGCA AGGCCAAGCT GGGCGACTCC CACGACCTGC AGCGCTTCCT CAGCGACTTC 
CGGGACCTCA TGTCTTGGAT CAATGGAATC CGAGGTCTGG TGTCCTCAGA CGAGCTAGCC 
AAGGATGTCA CTGGAGCTGA GGCCCTGCTG GAGCGACACC AGGAACACCG GACAGAAATT 
GATGCCAGAG CTGGCACTTT CCAGGCATTC GAGCAGTTTG GGCAGCAGCT GTTGGCTCAT 
GGGCACTACG CCAGCCCCGA GATCAAGGAG AAGCTCGATG TTCTAGACCA GGAGCGCGCA 
GACCTCGAGA AGGCCTGGGT CCAGCGCAGG ATGATGCTGG ATCAGTGCCT TGAATTGCAG 
CTGTTCCATC GGGATTGCGA GCAGGCCGAG AACTGGATGG CTGCCCGGGA GGCCTTCCTG 
AACACAGAAG ACAAAGGGGA CTCGCTAGAC AGCGTGGAGG CTCTGATCAA AAAACACGAG 
GACTTTGACA AAGCAATTAA TGTCCAGGAG GAGAAGATTG CTGCCCTGCA GTCCTTTGCT 
GACCAGCTCA TTGCTGGCGG TCACTACGCC AAAGGCGACA TCTCCAGCCG GCGCAATGAG 
GTTTTGGACA GGTGGCGCCG TCTAAAAGCT CAGATGATTG AGAAGAGGTC AAAGCTGGGA 
GAATCGCAGA CCCTCCAGCA GTTCAGCCGG GATGTAGACG AGATTGAAGC TTGGATCAGT 
GAAAAATTAC AGACTGCAAG TGATGAGTCA TATAAGGACC CCACCAACAT CCAGCTTTCC 
AAGCTGCTGA GCAAGCATCA GAAGCACCAG GCCTTCGAGG CAGAACTGCA CGCCAATGCG 
GACCGGATCC GCGGGGTCAT CGACGTGGGC AACTCCCTCA TTGACCGTGG GGCCTGTGCC 
GGCAGCGAGG ATGCCGTCAA GGCCCGGCTG GCCGCCTTAG CTGACCAGTG GCAGTTCCTG 
GTGCAGAAGT CGGCTGAGAA GAGCCAGAAG CTGAAAGAAG CCAACAAACA GCAGAACTTC 
AACACTGGCA TCAAGGACTT CGACTTCTGG CTCTCTGAGG TGGAAGCCCT GCTGGCCTCT 
GAGGATTATG GCAAAGACCT GGCTTCTGTG AACAACTTGC TAAAAAAACA TCAGCTGCTG 
GAGGCAGATA TCTCTGCCCA CGAGGACCGC CTGAAGGACC TCAACAGCCA GGCGGACAGC 
CTGATGACCA GCAGCGCCTT CGACACCTCC CAGGTGAAGG ATAAGCGGGA CACCATCAAC 
AGCCGTTTCC AGAAGATCAA GAGCATGGCC GCCTCCCGGC GAGCTAGGCT GAATGAGTCC 
CACCGCCTGC ACCAGTTCTT CCGGGACATG GACGATGAGG AGTCCTGGAT CAAGGAGAAG 
AAGCTGCTGG TGAGCTCAGA GGACTACGGC CGCGACCTGA CGGGCGTCCA GAACCTGAGG 
AAGAAGCACA AACGGCTGGA GGCAGAGCTG GCTGCCCACG AGCCGGCCAT TCAGGGTGTC 
CTGGACACTG GAAAGAAGCT GTCTGATGAC AACACCATCG GGAAGGAGGA GATTCAGCAG 
CGCCTGGCGC AGTTTGTGGA GCACTGGAAG GAGCTGAAGC AGCTGGCAGC TGCTCGGGGC 
CAGCGGCTAG AAGAATCTTT GGAATATCAG CAGTTTGTGG CCAACGTGGA GGAGGAGGAA 
GCCTGGATCA ATGAGAAGAT GACCCTGGTG GCCAGTGAGG ATTACGGAGA CACTCTTGCT 
GCCATCCAGG GCTTGCTGAA GAAACACGAA GCCTTCGAGA CGGACTTCAC TGTCCACAAG 
GACCGGGTGA ATGACGTCTG CACTAACGGA CAAGACCTGG TTAAGAAGAA CAATCACCAT 
GAGGAGAACA TCTCTTCCAA GATGAAGGGC TTGAACGGGA AAGTGTCAGA CTTGGAGAAG 
GCTGCCTCCC AGAGGAAGGC AAAGCTCGAC GAGAACTCGG CCTTCCTCCA GTTCAACTGG 
AAGGCCGATG TGGTGGAGTC CTGGATTGGT GAAAAGGAGA ACAGCTTGAA GACAGATGAT 
TATGGTCGAG ATCTGTCTTC TGTCCAAACT CTCCTCACCA AACAGGAAAC CTTCGATGCT 
GGCCTGCAGG CCTTCCAGCA GGAGGGCATC GCCAACATCA CTGCCCTCAA AGATCAGCTG 
CTGGCCGCCA AGCATGTTCA GTCCAAGGCC ATCGAGGCCC GCCACGCTGC CCTCATGAAG 
AGATGGAGCC AGCTCCTGGC CAATTCGGCC ACCCGGAAGA AGAAGCTGCT GGAGGCACAG 
AGTCACTTCC GCAAGGTGGA GGATCTCTTC CTGACCTTTG CCAAAAAGGC TTCAGCCTTC 
AACAGCTGGT TTGAAAATGC AGAGGAGGAC TTAACAGACC CAGTGCGCTG CAACTCCCTG 
GAAGAGATCA AGGCCTTGAG AGAGGCCCAC GATGCCTTCC GCTCCTCGCT GAGCTCAGCC 
CAGGCCGACT TCAACCAGCT GGCTGAGCTG GACCGCCAGA TCAAGAGCTT CCGCGTGGCC 
TCCAACCCCT ACACCTGGTT CACCATGGAG GCCCTGGAGG AGACCTGGAG GAACCTTCAG 
AAGATCATCA AGGAAAGGGA GCTGGAGCTG CAGAAGGAGC AGCGGCGGCA GGAGGAGAAC 
GACAAGCTGC GCCAGGAGTT CGCCCAGCAT GCCAACGCCT TCCACCAGTG GATCCAGGAG 
ACCAGGTCCT GCATGGTGGA AGAATCTGGG ACCCTTGAAT CGCAGCTTGA AGCTACCAAA 
CGCAAACACC AGGAGATTCG AGCCATGAGA AGTCAGCTCA AGAAGATTGA AGACCTGGGG 
GCGGCCATGG AGGAAGCGCT GATCCTGGAC AACAAGTACA CGGAGCACAG CACCGTGGGC 
CTGGCCCAGC AGTGGGACCA GCTCGACCAG CTGGGCATGC GGATGCAGCA CAACCTGGAG 
CAGCAGATCC AGGCCAGGAA CACGACAGGA GTGACCGAGG AGGCCCTCAA AGAATTCAGC 
ATGATGTTCA AACACTTCGA CAAGGACAAG TCCGGCCGGC TGAATCACCA GGAGTTCAAA 
TCCTGTCTGC GCTCCCTGGG CTATGACCTG CCCATGGTGG AGGAAGGGGA GCCCGACCCT 
GAGTTCGAGG CCATCTTGGA TACTGTGGAT CCTAACAGGG ACGGCCACGT CTCCCTGCAA 
GAGTACATGG CCTTTATGAT CAGCCGTGAG ACCGAGAATG TCAAGTCCAG CGAGGAGATT 
GAGAGCGCCT TCCGGGCCCT GAGCTCCGAA GGGAAGCCTT ATGTGACCAA GGAGGAGCTG 
TACCAGAACC TGACTCGGGA ACAAGCCGAC TACTGCGTCT CCCACATGAA GCCCTACGTG 
GACGGCAAGG GCCGCGAACT CCCCACTGCT TTCGACTACG TTGAGTTCAC CCGCTCGCTC 
TTCGTGAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_027822162.1
CDS	71..7522
Translation	MLSSFRKRRVQKMDPSGVKVLETAEDIQERRQQVLDRYHRFKELSTLRRQKLEDSYRFQFFQRDAEELEKWIQEKLQIASDENYKDPTNLQGKLQKHQAFEAEVQANSGAIVKLDETGNLMISEGHFASETIRTRLMELHRQWELLLEKMREKGIKLLQAQKLVQYLRECEDVMDWINDKEAIVTSEELGQDLEHVEVLQKKFEEFQTDMAAHEERVNEVNQFAAKLIQEQHPEEELIKTKEDEVNAAWQRLKGLALQRQGKLFGAAEVQRFNRDVDETISWIKEKEQLMASDDFGRDLASVQALLRKHEGLERDLAALEDKVKALCAEADRLQQSHPLSATQIQVKREELITNWEQIRTLAAERHARLNDSYRLQRFLADFRDLTSWVTEMKALINADELANDVAGAEALLDRHQEHKGEIDAHEDSFKSADESGQALLAAGHYASDEVREKLTILSEERAALLELWELRRQQYEQCMDLQLFYRDTEQVDNWMSKQEAFLLNEDLGDSLDSVEALLKKHEDFEKSLSAQEEKITALDEFATKLIQNNHYAMEDVATRRDALLSRRNALHERAMYRRAQLADSFHLQQFFRDSDELKSWVNEKMKTATDEAYKDPSNLQGKVQKHQAFEAELSANQSRIDALEKAGQKLIDVDHYAKDEVATRMNEVISLWKKLLEATELKGIKLREANQQQQFNRNVEDIELWLYEVEGHLASDDYGKDLTNVQNLQKKHALLEADVAAHQDRIDGITIQARQFQDAGHFDAENIKKKQEALVARYEALKEPMVARKQKLADSLRLQQLFRDVEDEETWIREKEPIAASTNRGKDLIGVQNLLKKHQALQAEIAGHEPRIKAVTQKGNAMVEEGHFAAEDVKAKLHELNQKWESLKSKASQRRQDLEDSLQAQQYFADANEAESWMREKEPIVGSTDYGKDEDSAEALLKKHEALMSDLSAYGSSIQALREQAQSCRQQVAPMDDETGKELVLALYDYQEKSPREVTMKKGDILTLLNSTNKDWWKVEVNDRQGFVPAAYVKKLDPAQSASRENLLEEQGSIALRQEQIDNQTRVTKEAGSVSLRMKQVEELYHSLLELGEKRKGMLEKSCKKFMLFREANELQQWINEKEAALTSEEVGADLEQVEVLQKKFDDFQKDLKANESRLKDINKVAEDLESEGLMAEEVQAVQQQEVYGAMPRDESDSKTASPWKSARLMVHTVATFNSIKELNERWRSLQQLAEERSQLLGSAHEVQRFHRDADETKEWIEEKNQALNTDNYGHDLASVQALQRKHEGFERDLAALGDKVNSLGETAERLIQSHPESAEDLQEKCTELNQAWSSLGKRADQRKAKLGDSHDLQRFLSDFRDLMSWINGIRGLVSSDELAKDVTGAEALLERHQEHRTEIDARAGTFQAFEQFGQQLLAHGHYASPEIKEKLDVLDQERADLEKAWVQRRMMLDQCLELQLFHRDCEQAENWMAAREAFLNTEDKGDSLDSVEALIKKHEDFDKAINVQEEKIAALQSFADQLIAGGHYAKGDISSRRNEVLDRWRRLKAQMIEKRSKLGESQTLQQFSRDVDEIEAWISEKLQTASDESYKDPTNIQLSKLLSKHQKHQAFEAELHANADRIRGVIDVGNSLIDRGACAGSEDAVKARLAALADQWQFLVQKSAEKSQKLKEANKQQNFNTGIKDFDFWLSEVEALLASEDYGKDLASVNNLLKKHQLLEADISAHEDRLKDLNSQADSLMTSSAFDTSQVKDKRDTINSRFQKIKSMAASRRARLNESHRLHQFFRDMDDEESWIKEKKLLVSSEDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQFVEHWKELKQLAAARGQRLEESLEYQQFVANVEEEEAWINEKMTLVASEDYGDTLAAIQGLLKKHEAFETDFTVHKDRVNDVCTNGQDLVKKNNHHEENISSKMKGLNGKVSDLEKAASQRKAKLDENSAFLQFNWKADVVESWIGEKENSLKTDDYGRDLSSVQTLLTKQETFDAGLQAFQQEGIANITALKDQLLAAKHVQSKAIEARHAALMKRWSQLLANSATRKKKLLEAQSHFRKVEDLFLTFAKKASAFNSWFENAEEDLTDPVRCNSLEEIKALREAHDAFRSSLSSAQADFNQLAELDRQIKSFRVASNPYTWFTMEALEETWRNLQKIIKERELELQKEQRRQEENDKLRQEFAQHANAFHQWIQETRSCMVEESGTLESQLEATKRKHQEIRAMRSQLKKIEDLGAAMEEALILDNKYTEHSTVGLAQQWDQLDQLGMRMQHNLEQQIQARNTTGVTEEALKEFSMMFKHFDKDKSGRLNHQEFKSCLRSLGYDLPMVEEGEPDPEFEAILDTVDPNRDGHVSLQEYMAFMISRETENVKSSEEIESAFRALSSEGKPYVTKEELYQNLTREQADYCVSHMKPYVDGKGRELPTAFDYVEFTRSLFVN

Target ORF information:

RefSeq Version	XM_027966361.1
Organism	Ovis aries(sheep)
Definition	Ovis aries spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027966361.1

ORF Insert Sequence:

ATGCTGTCAT CGTTTCGTAA ACGCAGAGTC CAGAAAATGG ACCCAAGCGG GGTCAAAGTG 
CTGGAGACAG CCGAGGACAT CCAGGAGAGG CGGCAGCAGG TCCTGGACAG ATACCACCGC 
TTCAAGGAGC TCTCGACCCT GAGGCGCCAG AAGCTTGAAG ACTCCTACCG ATTCCAGTTT 
TTCCAAAGAG ATGCTGAGGA GCTGGAGAAA TGGATTCAGG AGAAGCTTCA GATTGCCTCG 
GATGAGAATT ACAAAGACCC AACCAACCTG CAGGGCAAGC TGCAGAAGCA TCAAGCCTTC 
GAAGCCGAGG TGCAGGCCAA CTCGGGTGCC ATCGTGAAGC TTGATGAGAC CGGTAACCTG 
ATGATCTCAG AAGGGCACTT CGCGTCCGAA ACCATCCGGA CTCGTCTGAT GGAGCTGCAC 
CGCCAGTGGG AATTGCTTCT GGAGAAGATG CGGGAGAAAG GCATCAAGCT GCTGCAGGCC 
CAGAAGCTGG TGCAGTACCT GCGGGAGTGT GAGGACGTGA TGGACTGGAT CAATGACAAG 
GAAGCCATTG TGACATCTGA GGAGCTGGGC CAGGACCTGG AGCACGTGGA GGTGTTACAG 
AAGAAATTCG AAGAGTTTCA GACAGACATG GCTGCCCACG AAGAGAGAGT CAATGAAGTG 
AACCAGTTTG CTGCCAAGCT CATTCAGGAG CAGCACCCTG AGGAGGAGCT GATCAAGACA 
AAGGAGGACG AGGTGAATGC AGCCTGGCAG CGGCTGAAGG GCCTGGCCCT CCAGCGGCAG 
GGGAAGCTGT TCGGGGCAGC TGAGGTCCAA CGCTTTAACA GGGATGTGGA CGAGACCATT 
AGCTGGATTA AGGAGAAGGA ACAGTTAATG GCCTCTGATG ACTTTGGCCG GGACCTGGCC 
AGTGTCCAGG CTCTACTGCG GAAACACGAG GGTTTGGAGA GAGATCTTGC TGCCTTGGAG 
GACAAGGTCA AAGCCCTGTG TGCCGAGGCT GACCGCCTGC AGCAGTCCCA CCCTCTGAGT 
GCCACCCAGA TCCAAGTGAA GCGAGAGGAG CTGATCACCA ACTGGGAGCA GATCCGCACC 
CTGGCCGCGG AGAGACACGC ACGTCTCAAC GATTCCTACA GGCTTCAGCG CTTCCTTGCT 
GACTTCCGAG ACCTCACCAG CTGGGTCACT GAGATGAAAG CCCTCATCAA TGCAGATGAA 
CTTGCCAATG ATGTGGCCGG GGCCGAAGCC CTGCTGGATA GGCATCAGGA GCACAAGGGT 
GAAATTGACG CTCATGAAGA TAGCTTTAAA TCTGCAGATG AGTCTGGACA GGCCCTGCTT 
GCAGCTGGTC ACTATGCCTC AGATGAAGTG AGGGAGAAGC TGACCATCCT GTCCGAGGAG 
CGGGCCGCGC TACTAGAGCT CTGGGAGCTG CGACGGCAGC AATATGAGCA GTGCATGGAC 
CTGCAGCTCT TCTACCGCGA CACGGAGCAG GTGGACAACT GGATGAGCAA ACAGGAGGCG 
TTCCTGTTGA ACGAAGACCT GGGTGATTCC TTGGATAGTG TGGAAGCGCT TCTCAAGAAG 
CACGAAGACT TTGAGAAGTC CCTCAGCGCC CAGGAGGAGA AGATCACAGC CTTAGATGAG 
TTTGCCACCA AGCTGATTCA GAACAACCAC TACGCGATGG AAGATGTGGC CACCCGGCGA 
GACGCACTGC TGAGCCGCCG CAACGCCCTC CATGAGAGAG CCATGTACCG CCGTGCCCAG 
CTTGCAGATT CCTTCCACTT GCAGCAGTTT TTCCGTGACT CTGATGAGCT CAAGAGTTGG 
GTCAACGAGA AGATGAAAAC AGCCACAGAT GAAGCTTATA AAGACCCATC CAACCTACAA 
GGGAAAGTGC AGAAGCACCA GGCCTTTGAG GCCGAGCTCT CAGCAAACCA GAGCCGCATC 
GACGCCTTGG AGAAAGCTGG GCAGAAGCTG ATCGACGTTG ACCACTATGC CAAGGATGAG 
GTGGCGACTC GGATGAATGA AGTTATCAGT CTGTGGAAGA AGCTGCTGGA GGCCACGGAG 
CTGAAAGGCA TAAAGCTCCG TGAGGCTAAC CAGCAGCAGC AGTTTAACCG CAACGTTGAG 
GACATCGAGC TGTGGCTGTA CGAAGTGGAA GGTCATCTGG CCTCGGATGA CTACGGCAAA 
GACCTCACCA ACGTCCAGAA CCTCCAGAAA AAGCATGCCC TGCTGGAGGC TGACGTGGCC 
GCTCACCAGG ATCGCATCGA TGGCATCACT ATCCAAGCCC GCCAGTTCCA GGATGCGGGC 
CACTTTGATG CTGAAAACAT CAAGAAGAAG CAGGAGGCCC TGGTAGCTCG CTACGAGGCA 
CTCAAGGAGC CCATGGTTGC CCGGAAGCAG AAGCTGGCCG ATTCCCTGCG GTTGCAGCAG 
CTCTTCCGTG ACGTTGAGGA TGAGGAGACG TGGATTCGGG AGAAAGAACC TATTGCCGCC 
TCCACCAACA GAGGCAAAGA TTTAATTGGA GTCCAGAACC TGCTAAAGAA ACACCAAGCG 
CTACAAGCAG AAATTGCTGG CCACGAGCCC CGCATCAAAG CAGTCACACA GAAGGGGAAC 
GCCATGGTAG AGGAGGGCCA TTTTGCTGCC GAGGACGTGA AGGCCAAGCT CCATGAACTG 
AACCAGAAGT GGGAGTCGCT GAAGTCCAAG GCCTCCCAGC GACGGCAGGA CCTGGAGGAC 
TCGCTGCAGG CCCAGCAGTA CTTCGCTGAT GCCAACGAGG CCGAGTCCTG GATGCGGGAG 
AAGGAGCCCA TCGTGGGCAG CACCGACTAT GGCAAAGATG AGGACTCTGC CGAGGCTCTG 
CTAAAGAAGC ACGAGGCATT GATGTCGGAT CTCAGCGCCT ATGGCAGCAG CATCCAGGCC 
CTGCGAGAGC AGGCCCAGTC GTGCCGGCAA CAAGTGGCCC CCATGGATGA CGAGACTGGG 
AAGGAGCTGG TCTTGGCGCT CTATGACTAT CAGGAGAAGA GTCCCCGAGA GGTCACCATG 
AAGAAAGGAG ACATTCTCAC CTTACTCAAC AGCACCAACA AGGACTGGTG GAAAGTGGAA 
GTGAACGACC GGCAGGGCTT CGTGCCGGCC GCCTACGTGA AGAAGCTGGA CCCAGCGCAG 
TCGGCCTCCC GGGAGAACCT CCTGGAAGAG CAGGGCAGCA TCGCGCTGCG GCAGGAGCAG 
ATTGACAACC AGACGCGCGT AACTAAGGAG GCCGGCAGCG TGTCTCTGCG TATGAAACAG 
GTGGAAGAAC TGTACCATTC TCTGCTGGAA CTGGGAGAGA AGCGTAAAGG CATGTTGGAG 
AAGAGCTGTA AGAAGTTCAT GCTGTTCCGT GAAGCGAACG AGCTGCAGCA GTGGATCAAC 
GAGAAGGAAG CGGCCCTGAC AAGCGAGGAG GTCGGCGCGG ACTTGGAGCA GGTCGAAGTG 
CTGCAGAAGA AGTTTGACGA CTTCCAGAAG GATCTCAAGG CCAATGAGTC ACGGCTGAAG 
GACATTAACA AGGTGGCTGA AGACCTGGAG TCGGAAGGCC TGATGGCGGA GGAGGTGCAG 
GCCGTGCAGC AGCAGGAGGT GTATGGCGCG ATGCCCAGGG ATGAATCTGA TTCCAAGACA 
GCCTCCCCGT GGAAGTCTGC TCGCCTGATG GTTCACACCG TGGCCACCTT TAATTCCATC 
AAGGAGCTCA ATGAGCGCTG GCGGTCCCTG CAGCAGCTGG CTGAGGAGCG GAGCCAACTC 
TTGGGCAGCG CCCACGAAGT GCAGCGGTTC CACAGAGATG CTGACGAAAC CAAAGAGTGG 
ATCGAGGAGA AGAACCAGGC TCTAAACACA GACAACTATG GCCACGACCT GGCCAGCGTC 
CAGGCCCTGC AGCGCAAGCA TGAGGGCTTC GAGAGGGACC TCGCGGCCCT TGGCGACAAG 
GTGAACTCGC TCGGGGAGAC AGCCGAGCGC CTGATCCAGT CCCACCCCGA GTCGGCAGAG 
GACCTGCAGG AGAAGTGCAC TGAGCTGAAC CAGGCATGGA GCAGCCTGGG CAAGCGTGCC 
GACCAGCGCA AGGCCAAGCT GGGCGACTCC CACGACCTGC AGCGCTTCCT CAGCGACTTC 
CGGGACCTCA TGTCTTGGAT CAATGGAATC CGAGGTCTGG TGTCCTCAGA CGAGCTAGCC 
AAGGATGTCA CTGGAGCTGA GGCCCTGCTG GAGCGACACC AGGAACACCG GACAGAAATT 
GATGCCAGAG CTGGCACTTT CCAGGCATTC GAGCAGTTTG GGCAGCAGCT GTTGGCTCAT 
GGGCACTACG CCAGCCCCGA GATCAAGGAG AAGCTCGATG TTCTAGACCA GGAGCGCGCA 
GACCTCGAGA AGGCCTGGGT CCAGCGCAGG ATGATGCTGG ATCAGTGCCT TGAATTGCAG 
CTGTTCCATC GGGATTGCGA GCAGGCCGAG AACTGGATGG CTGCCCGGGA GGCCTTCCTG 
AACACAGAAG ACAAAGGGGA CTCGCTAGAC AGCGTGGAGG CTCTGATCAA AAAACACGAG 
GACTTTGACA AAGCAATTAA TGTCCAGGAG GAGAAGATTG CTGCCCTGCA GTCCTTTGCT 
GACCAGCTCA TTGCTGGCGG TCACTACGCC AAAGGCGACA TCTCCAGCCG GCGCAATGAG 
GTTTTGGACA GGTGGCGCCG TCTAAAAGCT CAGATGATTG AGAAGAGGTC AAAGCTGGGA 
GAATCGCAGA CCCTCCAGCA GTTCAGCCGG GATGTAGACG AGATTGAAGC TTGGATCAGT 
GAAAAATTAC AGACTGCAAG TGATGAGTCA TATAAGGACC CCACCAACAT CCAGCTTTCC 
AAGCTGCTGA GCAAGCATCA GAAGCACCAG GCCTTCGAGG CAGAACTGCA CGCCAATGCG 
GACCGGATCC GCGGGGTCAT CGACGTGGGC AACTCCCTCA TTGACCGTGG GGCCTGTGCC 
GGCAGCGAGG ATGCCGTCAA GGCCCGGCTG GCCGCCTTAG CTGACCAGTG GCAGTTCCTG 
GTGCAGAAGT CGGCTGAGAA GAGCCAGAAG CTGAAAGAAG CCAACAAACA GCAGAACTTC 
AACACTGGCA TCAAGGACTT CGACTTCTGG CTCTCTGAGG TGGAAGCCCT GCTGGCCTCT 
GAGGATTATG GCAAAGACCT GGCTTCTGTG AACAACTTGC TAAAAAAACA TCAGCTGCTG 
GAGGCAGATA TCTCTGCCCA CGAGGACCGC CTGAAGGACC TCAACAGCCA GGCGGACAGC 
CTGATGACCA GCAGCGCCTT CGACACCTCC CAGGTGAAGG ATAAGCGGGA CACCATCAAC 
AGCCGTTTCC AGAAGATCAA GAGCATGGCC GCCTCCCGGC GAGCTAGGCT GAATGAGTCC 
CACCGCCTGC ACCAGTTCTT CCGGGACATG GACGATGAGG AGTCCTGGAT CAAGGAGAAG 
AAGCTGCTGG TGAGCTCAGA GGACTACGGC CGCGACCTGA CGGGCGTCCA GAACCTGAGG 
AAGAAGCACA AACGGCTGGA GGCAGAGCTG GCTGCCCACG AGCCGGCCAT TCAGGGTGTC 
CTGGACACTG GAAAGAAGCT GTCTGATGAC AACACCATCG GGAAGGAGGA GATTCAGCAG 
CGCCTGGCGC AGTTTGTGGA GCACTGGAAG GAGCTGAAGC AGCTGGCAGC TGCTCGGGGC 
CAGCGGCTAG AAGAATCTTT GGAATATCAG CAGTTTGTGG CCAACGTGGA GGAGGAGGAA 
GCCTGGATCA ATGAGAAGAT GACCCTGGTG GCCAGTGAGG ATTACGGAGA CACTCTTGCT 
GCCATCCAGG GCTTGCTGAA GAAACACGAA GCCTTCGAGA CGGACTTCAC TGTCCACAAG 
GACCGGGTGA ATGACGTCTG CACTAACGGA CAAGACCTGG TTAAGAAGAA CAATCACCAT 
GAGGAGAACA TCTCTTCCAA GATGAAGGGC TTGAACGGGA AAGTGTCAGA CTTGGAGAAG 
GCTGCCTCCC AGAGGAAGGC AAAGCTCGAC GAGAACTCGG CCTTCCTCCA GTTCAACTGG 
AAGGCCGATG TGGTGGAGTC CTGGATTGGT GAAAAGGAGA ACAGCTTGAA GACAGATGAT 
TATGGTCGAG ATCTGTCTTC TGTCCAAACT CTCCTCACCA AACAGGAAAC CTTCGATGCT 
GGCCTGCAGG CCTTCCAGCA GGAGGGCATC GCCAACATCA CTGCCCTCAA AGATCAGCTG 
CTGGCCGCCA AGCATGTTCA GTCCAAGGCC ATCGAGGCCC GCCACGCTGC CCTCATGAAG 
AGATGGAGCC AGCTCCTGGC CAATTCGGCC ACCCGGAAGA AGAAGCTGCT GGAGGCACAG 
AGTCACTTCC GCAAGGTGGA GGATCTCTTC CTGACCTTTG CCAAAAAGGC TTCAGCCTTC 
AACAGCTGGT TTGAAAATGC AGAGGAGGAC TTAACAGACC CAGTGCGCTG CAACTCCCTG 
GAAGAGATCA AGGCCTTGAG AGAGGCCCAC GATGCCTTCC GCTCCTCGCT GAGCTCAGCC 
CAGGCCGACT TCAACCAGCT GGCTGAGCTG GACCGCCAGA TCAAGAGCTT CCGCGTGGCC 
TCCAACCCCT ACACCTGGTT CACCATGGAG GCCCTGGAGG AGACCTGGAG GAACCTTCAG 
AAGATCATCA AGGAAAGGGA GCTGGAGCTG CAGAAGGAGC AGCGGCGGCA GGAGGAGAAC 
GACAAGCTGC GCCAGGAGTT CGCCCAGCAT GCCAACGCCT TCCACCAGTG GATCCAGGAG 
ACCAGGTCCT GCATGGTGGA AGAATCTGGG ACCCTTGAAT CGCAGCTTGA AGCTACCAAA 
CGCAAACACC AGGAGATTCG AGCCATGAGA AGTCAGCTCA AGAAGATTGA AGACCTGGGG 
GCGGCCATGG AGGAAGCGCT GATCCTGGAC AACAAGTACA CGGAGCACAG CACCGTGGGC 
CTGGCCCAGC AGTGGGACCA GCTCGACCAG CTGGGCATGC GGATGCAGCA CAACCTGGAG 
CAGCAGATCC AGGCCAGGAA CACGACAGGA GTGACCGAGG AGGCCCTCAA AGAATTCAGC 
ATGATGTTCA AACACTTCGA CAAGGACAAG TCCGGCCGGC TGAATCACCA GGAGTTCAAA 
TCCTGTCTGC GCTCCCTGGG CTATGACCTG CCCATGGTGG AGGAAGGGGA GCCCGACCCT 
GAGTTCGAGG CCATCTTGGA TACTGTGGAT CCTAACAGGG ACGGCCACGT CTCCCTGCAA 
GAGTACATGG CCTTTATGAT CAGCCGTGAG ACCGAGAATG TCAAGTCCAG CGAGGAGATT 
GAGAGCGCCT TCCGGGCCCT GAGCTCCGAA GGGAAGCCTT ATGTGACCAA GGAGGAGCTG 
TACCAGAACC TGACTCGGGA ACAAGCCGAC TACTGCGTCT CCCACATGAA GCCCTACGTG 
GACGGCAAGG GCCGCGAACT CCCCACTGCT TTCGACTACG TTGAGTTCAC CCGCTCGCTC 
TTCGTGAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTAN1 ( XM_027966362.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_027822163.1 Ovis aries spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X9, mRNA.

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CloneID	OSm76656
Clone ID Related Accession (Same CDS sequence)	XM_027966362.1
Accession Version	XM_027966362.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7410bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-05
Organism	Ovis aries(sheep)
Product	spectrin alpha chain, non-erythrocytic 1 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040254.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ovis aries Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTCAT CGTTTCGTAA ACGCAGAGTC CAGAAAATGG ACCCAAGCGG GGTCAAAGTG 
CTGGAGACAG CCGAGGACAT CCAGGAGAGG CGGCAGCAGG TCCTGGACAG ATACCACCGC 
TTCAAGGAGC TCTCGACCCT GAGGCGCCAG AAGCTTGAAG ACTCCTACCG ATTCCAGTTT 
TTCCAAAGAG ATGCTGAGGA GCTGGAGAAA TGGATTCAGG AGAAGCTTCA GATTGCCTCG 
GATGAGAATT ACAAAGACCC AACCAACCTG CAGGGCAAGC TGCAGAAGCA TCAAGCCTTC 
GAAGCCGAGG TGCAGGCCAA CTCGGGTGCC ATCGTGAAGC TTGATGAGAC CGGTAACCTG 
ATGATCTCAG AAGGGCACTT CGCGTCCGAA ACCATCCGGA CTCGTCTGAT GGAGCTGCAC 
CGCCAGTGGG AATTGCTTCT GGAGAAGATG CGGGAGAAAG GCATCAAGCT GCTGCAGGCC 
CAGAAGCTGG TGCAGTACCT GCGGGAGTGT GAGGACGTGA TGGACTGGAT CAATGACAAG 
GAAGCCATTG TGACATCTGA GGAGCTGGGC CAGGACCTGG AGCACGTGGA GGTGTTACAG 
AAGAAATTCG AAGAGTTTCA GACAGACATG GCTGCCCACG AAGAGAGAGT CAATGAAGTG 
AACCAGTTTG CTGCCAAGCT CATTCAGGAG CAGCACCCTG AGGAGGAGCT GATCAAGACA 
AAGGAGGACG AGGTGAATGC AGCCTGGCAG CGGCTGAAGG GCCTGGCCCT CCAGCGGCAG 
GGGAAGCTGT TCGGGGCAGC TGAGGTCCAA CGCTTTAACA GGGATGTGGA CGAGACCATT 
AGCTGGATTA AGGAGAAGGA ACAGTTAATG GCCTCTGATG ACTTTGGCCG GGACCTGGCC 
AGTGTCCAGG CTCTACTGCG GAAACACGAG GGTTTGGAGA GAGATCTTGC TGCCTTGGAG 
GACAAGGTCA AAGCCCTGTG TGCCGAGGCT GACCGCCTGC AGCAGTCCCA CCCTCTGAGT 
GCCACCCAGA TCCAAGTGAA GCGAGAGGAG CTGATCACCA ACTGGGAGCA GATCCGCACC 
CTGGCCGCGG AGAGACACGC ACGTCTCAAC GATTCCTACA GGCTTCAGCG CTTCCTTGCT 
GACTTCCGAG ACCTCACCAG CTGGGTCACT GAGATGAAAG CCCTCATCAA TGCAGATGAA 
CTTGCCAATG ATGTGGCCGG GGCCGAAGCC CTGCTGGATA GGCATCAGGA GCACAAGGGT 
GAAATTGACG CTCATGAAGA TAGCTTTAAA TCTGCAGATG AGTCTGGACA GGCCCTGCTT 
GCAGCTGGTC ACTATGCCTC AGATGAAGTG AGGGAGAAGC TGACCATCCT GTCCGAGGAG 
CGGGCCGCGC TACTAGAGCT CTGGGAGCTG CGACGGCAGC AATATGAGCA GTGCATGGAC 
CTGCAGCTCT TCTACCGCGA CACGGAGCAG GTGGACAACT GGATGAGCAA ACAGGAGGCG 
TTCCTGTTGA ACGAAGACCT GGGTGATTCC TTGGATAGTG TGGAAGCGCT TCTCAAGAAG 
CACGAAGACT TTGAGAAGTC CCTCAGCGCC CAGGAGGAGA AGATCACAGC CTTAGATGAG 
TTTGCCACCA AGCTGATTCA GAACAACCAC TACGCGATGG AAGATGTGGC CACCCGGCGA 
GACGCACTGC TGAGCCGCCG CAACGCCCTC CATGAGAGAG CCATGTACCG CCGTGCCCAG 
CTTGCAGATT CCTTCCACTT GCAGCAGTTT TTCCGTGACT CTGATGAGCT CAAGAGTTGG 
GTCAACGAGA AGATGAAAAC AGCCACAGAT GAAGCTTATA AAGACCCATC CAACCTACAA 
GGGAAAGTGC AGAAGCACCA GGCCTTTGAG GCCGAGCTCT CAGCAAACCA GAGCCGCATC 
GACGCCTTGG AGAAAGCTGG GCAGAAGCTG ATCGACGTTG ACCACTATGC CAAGGATGAG 
GTGGCGACTC GGATGAATGA AGTTATCAGT CTGTGGAAGA AGCTGCTGGA GGCCACGGAG 
CTGAAAGGCA TAAAGCTCCG TGAGGCTAAC CAGCAGCAGC AGTTTAACCG CAACGTTGAG 
GACATCGAGC TGTGGCTGTA CGAAGTGGAA GGTCATCTGG CCTCGGATGA CTACGGCAAA 
GACCTCACCA ACGTCCAGAA CCTCCAGAAA AAGCATGCCC TGCTGGAGGC TGACGTGGCC 
GCTCACCAGG ATCGCATCGA TGGCATCACT ATCCAAGCCC GCCAGTTCCA GGATGCGGGC 
CACTTTGATG CTGAAAACAT CAAGAAGAAG CAGGAGGCCC TGGTAGCTCG CTACGAGGCA 
CTCAAGGAGC CCATGGTTGC CCGGAAGCAG AAGCTGGCCG ATTCCCTGCG GTTGCAGCAG 
CTCTTCCGTG ACGTTGAGGA TGAGGAGACG TGGATTCGGG AGAAAGAACC TATTGCCGCC 
TCCACCAACA GAGGCAAAGA TTTAATTGGA GTCCAGAACC TGCTAAAGAA ACACCAAGCG 
CTACAAGCAG AAATTGCTGG CCACGAGCCC CGCATCAAAG CAGTCACACA GAAGGGGAAC 
GCCATGGTAG AGGAGGGCCA TTTTGCTGCC GAGGACGTGA AGGCCAAGCT CCATGAACTG 
AACCAGAAGT GGGAGTCGCT GAAGTCCAAG GCCTCCCAGC GACGGCAGGA CCTGGAGGAC 
TCGCTGCAGG CCCAGCAGTA CTTCGCTGAT GCCAACGAGG CCGAGTCCTG GATGCGGGAG 
AAGGAGCCCA TCGTGGGCAG CACCGACTAT GGCAAAGATG AGGACTCTGC CGAGGCTCTG 
CTAAAGAAGC ACGAGGCATT GATGTCGGAT CTCAGCGCCT ATGGCAGCAG CATCCAGGCC 
CTGCGAGAGC AGGCCCAGTC GTGCCGGCAA CAAGTGGCCC CCATGGATGA CGAGACTGGG 
AAGGAGCTGG TCTTGGCGCT CTATGACTAT CAGGAGAAGA GTCCCCGAGA GGTCACCATG 
AAGAAAGGAG ACATTCTCAC CTTACTCAAC AGCACCAACA AGGACTGGTG GAAAGTGGAA 
GTGAACGACC GGCAGGGCTT CGTGCCGGCC GCCTACGTGA AGAAGCTGGA CCCAGCGCAG 
TCGGCCTCCC GGGAGAACCT CCTGGAAGAG CAGGGCAGCA TCGCGCTGCG GCAGGAGCAG 
ATTGACAACC AGTACCATTC TCTGCTGGAA CTGGGAGAGA AGCGTAAAGG CATGTTGGAG 
AAGAGCTGTA AGAAGTTCAT GCTGTTCCGT GAAGCGAACG AGCTGCAGCA GTGGATCAAC 
GAGAAGGAAG CGGCCCTGAC AAGCGAGGAG GTCGGCGCGG ACTTGGAGCA GGTCGAAGTG 
CTGCAGAAGA AGTTTGACGA CTTCCAGAAG GATCTCAAGG CCAATGAGTC ACGGCTGAAG 
GACATTAACA AGGTGGCTGA AGACCTGGAG TCGGAAGGCC TGATGGCGGA GGAGGTGCAG 
GCCGTGCAGC AGCAGGAGGT GTATGGCGCG ATGCCCAGGG ATGAATCTGA TTCCAAGACA 
GCCTCCCCGT GGAAGTCTGC TCGCCTGATG GTTCACACCG TGGCCACCTT TAATTCCATC 
AAGGAGCTCA ATGAGCGCTG GCGGTCCCTG CAGCAGCTGG CTGAGGAGCG GAGCCAACTC 
TTGGGCAGCG CCCACGAAGT GCAGCGGTTC CACAGAGATG CTGACGAAAC CAAAGAGTGG 
ATCGAGGAGA AGAACCAGGC TCTAAACACA GACAACTATG GCCACGACCT GGCCAGCGTC 
CAGGCCCTGC AGCGCAAGCA TGAGGGCTTC GAGAGGGACC TCGCGGCCCT TGGCGACAAG 
GTGAACTCGC TCGGGGAGAC AGCCGAGCGC CTGATCCAGT CCCACCCCGA GTCGGCAGAG 
GACCTGCAGG AGAAGTGCAC TGAGCTGAAC CAGGCATGGA GCAGCCTGGG CAAGCGTGCC 
GACCAGCGCA AGGCCAAGCT GGGCGACTCC CACGACCTGC AGCGCTTCCT CAGCGACTTC 
CGGGACCTCA TGTCTTGGAT CAATGGAATC CGAGGTCTGG TGTCCTCAGA CGAGCTAGCC 
AAGGATGTCA CTGGAGCTGA GGCCCTGCTG GAGCGACACC AGGAACACCG GACAGAAATT 
GATGCCAGAG CTGGCACTTT CCAGGCATTC GAGCAGTTTG GGCAGCAGCT GTTGGCTCAT 
GGGCACTACG CCAGCCCCGA GATCAAGGAG AAGCTCGATG TTCTAGACCA GGAGCGCGCA 
GACCTCGAGA AGGCCTGGGT CCAGCGCAGG ATGATGCTGG ATCAGTGCCT TGAATTGCAG 
CTGTTCCATC GGGATTGCGA GCAGGCCGAG AACTGGATGG CTGCCCGGGA GGCCTTCCTG 
AACACAGAAG ACAAAGGGGA CTCGCTAGAC AGCGTGGAGG CTCTGATCAA AAAACACGAG 
GACTTTGACA AAGCAATTAA TGTCCAGGAG GAGAAGATTG CTGCCCTGCA GTCCTTTGCT 
GACCAGCTCA TTGCTGGCGG TCACTACGCC AAAGGCGACA TCTCCAGCCG GCGCAATGAG 
GTTTTGGACA GGTGGCGCCG TCTAAAAGCT CAGATGATTG AGAAGAGGTC AAAGCTGGGA 
GAATCGCAGA CCCTCCAGCA GTTCAGCCGG GATGTAGACG AGATTGAAGC TTGGATCAGT 
GAAAAATTAC AGACTGCAAG TGATGAGTCA TATAAGGACC CCACCAACAT CCAGCTTTCC 
AAGCTGCTGA GCAAGCATCA GAAGCACCAG GCCTTCGAGG CAGAACTGCA CGCCAATGCG 
GACCGGATCC GCGGGGTCAT CGACGTGGGC AACTCCCTCA TTGACCGTGG GGCCTGTGCC 
GGCAGCGAGG ATGCCGTCAA GGCCCGGCTG GCCGCCTTAG CTGACCAGTG GCAGTTCCTG 
GTGCAGAAGT CGGCTGAGAA GAGCCAGAAG CTGAAAGAAG CCAACAAACA GCAGAACTTC 
AACACTGGCA TCAAGGACTT CGACTTCTGG CTCTCTGAGG TGGAAGCCCT GCTGGCCTCT 
GAGGATTATG GCAAAGACCT GGCTTCTGTG AACAACTTGC TAAAAAAACA TCAGCTGCTG 
GAGGCAGATA TCTCTGCCCA CGAGGACCGC CTGAAGGACC TCAACAGCCA GGCGGACAGC 
CTGATGACCA GCAGCGCCTT CGACACCTCC CAGGTGAAGG ATAAGCGGGA CACCATCAAC 
AGCCGTTTCC AGAAGATCAA GAGCATGGCC GCCTCCCGGC GAGCTAGGCT GAATGAGTCC 
CACCGCCTGC ACCAGTTCTT CCGGGACATG GACGATGAGG AGTCCTGGAT CAAGGAGAAG 
AAGCTGCTGG TGAGCTCAGA GGACTACGGC CGCGACCTGA CGGGCGTCCA GAACCTGAGG 
AAGAAGCACA AACGGCTGGA GGCAGAGCTG GCTGCCCACG AGCCGGCCAT TCAGGGTGTC 
CTGGACACTG GAAAGAAGCT GTCTGATGAC AACACCATCG GGAAGGAGGA GATTCAGCAG 
CGCCTGGCGC AGTTTGTGGA GCACTGGAAG GAGCTGAAGC AGCTGGCAGC TGCTCGGGGC 
CAGCGGCTAG AAGAATCTTT GGAATATCAG CAGTTTGTGG CCAACGTGGA GGAGGAGGAA 
GCCTGGATCA ATGAGAAGAT GACCCTGGTG GCCAGTGAGG ATTACGGAGA CACTCTTGCT 
GCCATCCAGG GCTTGCTGAA GAAACACGAA GCCTTCGAGA CGGACTTCAC TGTCCACAAG 
GACCGGGTGA ATGACGTCTG CACTAACGGA CAAGACCTGG TTAAGAAGAA CAATCACCAT 
GAGGAGAACA TCTCTTCCAA GATGAAGGGC TTGAACGGGA AAGTGTCAGA CTTGGAGAAG 
GCTGCCTCCC AGAGGAAGGC AAAGCTCGAC GAGAACTCGG CCTTCCTCCA GTTCAACTGG 
AAGGCCGATG TGGTGGAGTC CTGGATTGGT GAAAAGGAGA ACAGCTTGAA GACAGATGAT 
TATGGTCGAG ATCTGTCTTC TGTCCAAACT CTCCTCACCA AACAGGAAAC CTTCGATGCT 
GGCCTGCAGG CCTTCCAGCA GGAGGGCATC GCCAACATCA CTGCCCTCAA AGATCAGCTG 
CTGGCCGCCA AGCATGTTCA GTCCAAGGCC ATCGAGGCCC GCCACGCTGC CCTCATGAAG 
AGATGGAGCC AGCTCCTGGC CAATTCGGCC ACCCGGAAGA AGAAGCTGCT GGAGGCACAG 
AGTCACTTCC GCAAGGTGGA GGATCTCTTC CTGACCTTTG CCAAAAAGGC TTCAGCCTTC 
AACAGCTGGT TTGAAAATGC AGAGGAGGAC TTAACAGACC CAGTGCGCTG CAACTCCCTG 
GAAGAGATCA AGGCCTTGAG AGAGGCCCAC GATGCCTTCC GCTCCTCGCT GAGCTCAGCC 
CAGGCCGACT TCAACCAGCT GGCTGAGCTG GACCGCCAGA TCAAGAGCTT CCGCGTGGCC 
TCCAACCCCT ACACCTGGTT CACCATGGAG GCCCTGGAGG AGACCTGGAG GAACCTTCAG 
AAGATCATCA AGGAAAGGGA GCTGGAGCTG CAGAAGGAGC AGCGGCGGCA GGAGGAGAAC 
GACAAGCTGC GCCAGGAGTT CGCCCAGCAT GCCAACGCCT TCCACCAGTG GATCCAGGAG 
ACCAGAACGT ATCTCCTCGA TGGGTCCTGC ATGGTGGAAG AATCTGGGAC CCTTGAATCG 
CAGCTTGAAG CTACCAAACG CAAACACCAG GAGATTCGAG CCATGAGAAG TCAGCTCAAG 
AAGATTGAAG ACCTGGGGGC GGCCATGGAG GAAGCGCTGA TCCTGGACAA CAAGTACACG 
GAGCACAGCA CCGTGGGCCT GGCCCAGCAG TGGGACCAGC TCGACCAGCT GGGCATGCGG 
ATGCAGCACA ACCTGGAGCA GCAGATCCAG GCCAGGAACA CGACAGGAGT GACCGAGGAG 
GCCCTCAAAG AATTCAGCAT GATGTTCAAA CACTTCGACA AGGACAAGTC CGGCCGGCTG 
AATCACCAGG AGTTCAAATC CTGTCTGCGC TCCCTGGGCT ATGACCTGCC CATGGTGGAG 
GAAGGGGAGC CCGACCCTGA GTTCGAGGCC ATCTTGGATA CTGTGGATCC TAACAGGGAC 
GGCCACGTCT CCCTGCAAGA GTACATGGCC TTTATGATCA GCCGTGAGAC CGAGAATGTC 
AAGTCCAGCG AGGAGATTGA GAGCGCCTTC CGGGCCCTGA GCTCCGAAGG GAAGCCTTAT 
GTGACCAAGG AGGAGCTGTA CCAGAACCTG ACTCGGGAAC AAGCCGACTA CTGCGTCTCC 
CACATGAAGC CCTACGTGGA CGGCAAGGGC CGCGAACTCC CCACTGCTTT CGACTACGTT 
GAGTTCACCC GCTCGCTCTT CGTGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_027822163.1
CDS	70..7479
Translation	MLSSFRKRRVQKMDPSGVKVLETAEDIQERRQQVLDRYHRFKELSTLRRQKLEDSYRFQFFQRDAEELEKWIQEKLQIASDENYKDPTNLQGKLQKHQAFEAEVQANSGAIVKLDETGNLMISEGHFASETIRTRLMELHRQWELLLEKMREKGIKLLQAQKLVQYLRECEDVMDWINDKEAIVTSEELGQDLEHVEVLQKKFEEFQTDMAAHEERVNEVNQFAAKLIQEQHPEEELIKTKEDEVNAAWQRLKGLALQRQGKLFGAAEVQRFNRDVDETISWIKEKEQLMASDDFGRDLASVQALLRKHEGLERDLAALEDKVKALCAEADRLQQSHPLSATQIQVKREELITNWEQIRTLAAERHARLNDSYRLQRFLADFRDLTSWVTEMKALINADELANDVAGAEALLDRHQEHKGEIDAHEDSFKSADESGQALLAAGHYASDEVREKLTILSEERAALLELWELRRQQYEQCMDLQLFYRDTEQVDNWMSKQEAFLLNEDLGDSLDSVEALLKKHEDFEKSLSAQEEKITALDEFATKLIQNNHYAMEDVATRRDALLSRRNALHERAMYRRAQLADSFHLQQFFRDSDELKSWVNEKMKTATDEAYKDPSNLQGKVQKHQAFEAELSANQSRIDALEKAGQKLIDVDHYAKDEVATRMNEVISLWKKLLEATELKGIKLREANQQQQFNRNVEDIELWLYEVEGHLASDDYGKDLTNVQNLQKKHALLEADVAAHQDRIDGITIQARQFQDAGHFDAENIKKKQEALVARYEALKEPMVARKQKLADSLRLQQLFRDVEDEETWIREKEPIAASTNRGKDLIGVQNLLKKHQALQAEIAGHEPRIKAVTQKGNAMVEEGHFAAEDVKAKLHELNQKWESLKSKASQRRQDLEDSLQAQQYFADANEAESWMREKEPIVGSTDYGKDEDSAEALLKKHEALMSDLSAYGSSIQALREQAQSCRQQVAPMDDETGKELVLALYDYQEKSPREVTMKKGDILTLLNSTNKDWWKVEVNDRQGFVPAAYVKKLDPAQSASRENLLEEQGSIALRQEQIDNQYHSLLELGEKRKGMLEKSCKKFMLFREANELQQWINEKEAALTSEEVGADLEQVEVLQKKFDDFQKDLKANESRLKDINKVAEDLESEGLMAEEVQAVQQQEVYGAMPRDESDSKTASPWKSARLMVHTVATFNSIKELNERWRSLQQLAEERSQLLGSAHEVQRFHRDADETKEWIEEKNQALNTDNYGHDLASVQALQRKHEGFERDLAALGDKVNSLGETAERLIQSHPESAEDLQEKCTELNQAWSSLGKRADQRKAKLGDSHDLQRFLSDFRDLMSWINGIRGLVSSDELAKDVTGAEALLERHQEHRTEIDARAGTFQAFEQFGQQLLAHGHYASPEIKEKLDVLDQERADLEKAWVQRRMMLDQCLELQLFHRDCEQAENWMAAREAFLNTEDKGDSLDSVEALIKKHEDFDKAINVQEEKIAALQSFADQLIAGGHYAKGDISSRRNEVLDRWRRLKAQMIEKRSKLGESQTLQQFSRDVDEIEAWISEKLQTASDESYKDPTNIQLSKLLSKHQKHQAFEAELHANADRIRGVIDVGNSLIDRGACAGSEDAVKARLAALADQWQFLVQKSAEKSQKLKEANKQQNFNTGIKDFDFWLSEVEALLASEDYGKDLASVNNLLKKHQLLEADISAHEDRLKDLNSQADSLMTSSAFDTSQVKDKRDTINSRFQKIKSMAASRRARLNESHRLHQFFRDMDDEESWIKEKKLLVSSEDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQFVEHWKELKQLAAARGQRLEESLEYQQFVANVEEEEAWINEKMTLVASEDYGDTLAAIQGLLKKHEAFETDFTVHKDRVNDVCTNGQDLVKKNNHHEENISSKMKGLNGKVSDLEKAASQRKAKLDENSAFLQFNWKADVVESWIGEKENSLKTDDYGRDLSSVQTLLTKQETFDAGLQAFQQEGIANITALKDQLLAAKHVQSKAIEARHAALMKRWSQLLANSATRKKKLLEAQSHFRKVEDLFLTFAKKASAFNSWFENAEEDLTDPVRCNSLEEIKALREAHDAFRSSLSSAQADFNQLAELDRQIKSFRVASNPYTWFTMEALEETWRNLQKIIKERELELQKEQRRQEENDKLRQEFAQHANAFHQWIQETRTYLLDGSCMVEESGTLESQLEATKRKHQEIRAMRSQLKKIEDLGAAMEEALILDNKYTEHSTVGLAQQWDQLDQLGMRMQHNLEQQIQARNTTGVTEEALKEFSMMFKHFDKDKSGRLNHQEFKSCLRSLGYDLPMVEEGEPDPEFEAILDTVDPNRDGHVSLQEYMAFMISRETENVKSSEEIESAFRALSSEGKPYVTKEELYQNLTREQADYCVSHMKPYVDGKGRELPTAFDYVEFTRSLFVN

Target ORF information:

RefSeq Version	XM_027966362.1
Organism	Ovis aries(sheep)
Definition	Ovis aries spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027966362.1

ORF Insert Sequence:

ATGCTGTCAT CGTTTCGTAA ACGCAGAGTC CAGAAAATGG ACCCAAGCGG GGTCAAAGTG 
CTGGAGACAG CCGAGGACAT CCAGGAGAGG CGGCAGCAGG TCCTGGACAG ATACCACCGC 
TTCAAGGAGC TCTCGACCCT GAGGCGCCAG AAGCTTGAAG ACTCCTACCG ATTCCAGTTT 
TTCCAAAGAG ATGCTGAGGA GCTGGAGAAA TGGATTCAGG AGAAGCTTCA GATTGCCTCG 
GATGAGAATT ACAAAGACCC AACCAACCTG CAGGGCAAGC TGCAGAAGCA TCAAGCCTTC 
GAAGCCGAGG TGCAGGCCAA CTCGGGTGCC ATCGTGAAGC TTGATGAGAC CGGTAACCTG 
ATGATCTCAG AAGGGCACTT CGCGTCCGAA ACCATCCGGA CTCGTCTGAT GGAGCTGCAC 
CGCCAGTGGG AATTGCTTCT GGAGAAGATG CGGGAGAAAG GCATCAAGCT GCTGCAGGCC 
CAGAAGCTGG TGCAGTACCT GCGGGAGTGT GAGGACGTGA TGGACTGGAT CAATGACAAG 
GAAGCCATTG TGACATCTGA GGAGCTGGGC CAGGACCTGG AGCACGTGGA GGTGTTACAG 
AAGAAATTCG AAGAGTTTCA GACAGACATG GCTGCCCACG AAGAGAGAGT CAATGAAGTG 
AACCAGTTTG CTGCCAAGCT CATTCAGGAG CAGCACCCTG AGGAGGAGCT GATCAAGACA 
AAGGAGGACG AGGTGAATGC AGCCTGGCAG CGGCTGAAGG GCCTGGCCCT CCAGCGGCAG 
GGGAAGCTGT TCGGGGCAGC TGAGGTCCAA CGCTTTAACA GGGATGTGGA CGAGACCATT 
AGCTGGATTA AGGAGAAGGA ACAGTTAATG GCCTCTGATG ACTTTGGCCG GGACCTGGCC 
AGTGTCCAGG CTCTACTGCG GAAACACGAG GGTTTGGAGA GAGATCTTGC TGCCTTGGAG 
GACAAGGTCA AAGCCCTGTG TGCCGAGGCT GACCGCCTGC AGCAGTCCCA CCCTCTGAGT 
GCCACCCAGA TCCAAGTGAA GCGAGAGGAG CTGATCACCA ACTGGGAGCA GATCCGCACC 
CTGGCCGCGG AGAGACACGC ACGTCTCAAC GATTCCTACA GGCTTCAGCG CTTCCTTGCT 
GACTTCCGAG ACCTCACCAG CTGGGTCACT GAGATGAAAG CCCTCATCAA TGCAGATGAA 
CTTGCCAATG ATGTGGCCGG GGCCGAAGCC CTGCTGGATA GGCATCAGGA GCACAAGGGT 
GAAATTGACG CTCATGAAGA TAGCTTTAAA TCTGCAGATG AGTCTGGACA GGCCCTGCTT 
GCAGCTGGTC ACTATGCCTC AGATGAAGTG AGGGAGAAGC TGACCATCCT GTCCGAGGAG 
CGGGCCGCGC TACTAGAGCT CTGGGAGCTG CGACGGCAGC AATATGAGCA GTGCATGGAC 
CTGCAGCTCT TCTACCGCGA CACGGAGCAG GTGGACAACT GGATGAGCAA ACAGGAGGCG 
TTCCTGTTGA ACGAAGACCT GGGTGATTCC TTGGATAGTG TGGAAGCGCT TCTCAAGAAG 
CACGAAGACT TTGAGAAGTC CCTCAGCGCC CAGGAGGAGA AGATCACAGC CTTAGATGAG 
TTTGCCACCA AGCTGATTCA GAACAACCAC TACGCGATGG AAGATGTGGC CACCCGGCGA 
GACGCACTGC TGAGCCGCCG CAACGCCCTC CATGAGAGAG CCATGTACCG CCGTGCCCAG 
CTTGCAGATT CCTTCCACTT GCAGCAGTTT TTCCGTGACT CTGATGAGCT CAAGAGTTGG 
GTCAACGAGA AGATGAAAAC AGCCACAGAT GAAGCTTATA AAGACCCATC CAACCTACAA 
GGGAAAGTGC AGAAGCACCA GGCCTTTGAG GCCGAGCTCT CAGCAAACCA GAGCCGCATC 
GACGCCTTGG AGAAAGCTGG GCAGAAGCTG ATCGACGTTG ACCACTATGC CAAGGATGAG 
GTGGCGACTC GGATGAATGA AGTTATCAGT CTGTGGAAGA AGCTGCTGGA GGCCACGGAG 
CTGAAAGGCA TAAAGCTCCG TGAGGCTAAC CAGCAGCAGC AGTTTAACCG CAACGTTGAG 
GACATCGAGC TGTGGCTGTA CGAAGTGGAA GGTCATCTGG CCTCGGATGA CTACGGCAAA 
GACCTCACCA ACGTCCAGAA CCTCCAGAAA AAGCATGCCC TGCTGGAGGC TGACGTGGCC 
GCTCACCAGG ATCGCATCGA TGGCATCACT ATCCAAGCCC GCCAGTTCCA GGATGCGGGC 
CACTTTGATG CTGAAAACAT CAAGAAGAAG CAGGAGGCCC TGGTAGCTCG CTACGAGGCA 
CTCAAGGAGC CCATGGTTGC CCGGAAGCAG AAGCTGGCCG ATTCCCTGCG GTTGCAGCAG 
CTCTTCCGTG ACGTTGAGGA TGAGGAGACG TGGATTCGGG AGAAAGAACC TATTGCCGCC 
TCCACCAACA GAGGCAAAGA TTTAATTGGA GTCCAGAACC TGCTAAAGAA ACACCAAGCG 
CTACAAGCAG AAATTGCTGG CCACGAGCCC CGCATCAAAG CAGTCACACA GAAGGGGAAC 
GCCATGGTAG AGGAGGGCCA TTTTGCTGCC GAGGACGTGA AGGCCAAGCT CCATGAACTG 
AACCAGAAGT GGGAGTCGCT GAAGTCCAAG GCCTCCCAGC GACGGCAGGA CCTGGAGGAC 
TCGCTGCAGG CCCAGCAGTA CTTCGCTGAT GCCAACGAGG CCGAGTCCTG GATGCGGGAG 
AAGGAGCCCA TCGTGGGCAG CACCGACTAT GGCAAAGATG AGGACTCTGC CGAGGCTCTG 
CTAAAGAAGC ACGAGGCATT GATGTCGGAT CTCAGCGCCT ATGGCAGCAG CATCCAGGCC 
CTGCGAGAGC AGGCCCAGTC GTGCCGGCAA CAAGTGGCCC CCATGGATGA CGAGACTGGG 
AAGGAGCTGG TCTTGGCGCT CTATGACTAT CAGGAGAAGA GTCCCCGAGA GGTCACCATG 
AAGAAAGGAG ACATTCTCAC CTTACTCAAC AGCACCAACA AGGACTGGTG GAAAGTGGAA 
GTGAACGACC GGCAGGGCTT CGTGCCGGCC GCCTACGTGA AGAAGCTGGA CCCAGCGCAG 
TCGGCCTCCC GGGAGAACCT CCTGGAAGAG CAGGGCAGCA TCGCGCTGCG GCAGGAGCAG 
ATTGACAACC AGTACCATTC TCTGCTGGAA CTGGGAGAGA AGCGTAAAGG CATGTTGGAG 
AAGAGCTGTA AGAAGTTCAT GCTGTTCCGT GAAGCGAACG AGCTGCAGCA GTGGATCAAC 
GAGAAGGAAG CGGCCCTGAC AAGCGAGGAG GTCGGCGCGG ACTTGGAGCA GGTCGAAGTG 
CTGCAGAAGA AGTTTGACGA CTTCCAGAAG GATCTCAAGG CCAATGAGTC ACGGCTGAAG 
GACATTAACA AGGTGGCTGA AGACCTGGAG TCGGAAGGCC TGATGGCGGA GGAGGTGCAG 
GCCGTGCAGC AGCAGGAGGT GTATGGCGCG ATGCCCAGGG ATGAATCTGA TTCCAAGACA 
GCCTCCCCGT GGAAGTCTGC TCGCCTGATG GTTCACACCG TGGCCACCTT TAATTCCATC 
AAGGAGCTCA ATGAGCGCTG GCGGTCCCTG CAGCAGCTGG CTGAGGAGCG GAGCCAACTC 
TTGGGCAGCG CCCACGAAGT GCAGCGGTTC CACAGAGATG CTGACGAAAC CAAAGAGTGG 
ATCGAGGAGA AGAACCAGGC TCTAAACACA GACAACTATG GCCACGACCT GGCCAGCGTC 
CAGGCCCTGC AGCGCAAGCA TGAGGGCTTC GAGAGGGACC TCGCGGCCCT TGGCGACAAG 
GTGAACTCGC TCGGGGAGAC AGCCGAGCGC CTGATCCAGT CCCACCCCGA GTCGGCAGAG 
GACCTGCAGG AGAAGTGCAC TGAGCTGAAC CAGGCATGGA GCAGCCTGGG CAAGCGTGCC 
GACCAGCGCA AGGCCAAGCT GGGCGACTCC CACGACCTGC AGCGCTTCCT CAGCGACTTC 
CGGGACCTCA TGTCTTGGAT CAATGGAATC CGAGGTCTGG TGTCCTCAGA CGAGCTAGCC 
AAGGATGTCA CTGGAGCTGA GGCCCTGCTG GAGCGACACC AGGAACACCG GACAGAAATT 
GATGCCAGAG CTGGCACTTT CCAGGCATTC GAGCAGTTTG GGCAGCAGCT GTTGGCTCAT 
GGGCACTACG CCAGCCCCGA GATCAAGGAG AAGCTCGATG TTCTAGACCA GGAGCGCGCA 
GACCTCGAGA AGGCCTGGGT CCAGCGCAGG ATGATGCTGG ATCAGTGCCT TGAATTGCAG 
CTGTTCCATC GGGATTGCGA GCAGGCCGAG AACTGGATGG CTGCCCGGGA GGCCTTCCTG 
AACACAGAAG ACAAAGGGGA CTCGCTAGAC AGCGTGGAGG CTCTGATCAA AAAACACGAG 
GACTTTGACA AAGCAATTAA TGTCCAGGAG GAGAAGATTG CTGCCCTGCA GTCCTTTGCT 
GACCAGCTCA TTGCTGGCGG TCACTACGCC AAAGGCGACA TCTCCAGCCG GCGCAATGAG 
GTTTTGGACA GGTGGCGCCG TCTAAAAGCT CAGATGATTG AGAAGAGGTC AAAGCTGGGA 
GAATCGCAGA CCCTCCAGCA GTTCAGCCGG GATGTAGACG AGATTGAAGC TTGGATCAGT 
GAAAAATTAC AGACTGCAAG TGATGAGTCA TATAAGGACC CCACCAACAT CCAGCTTTCC 
AAGCTGCTGA GCAAGCATCA GAAGCACCAG GCCTTCGAGG CAGAACTGCA CGCCAATGCG 
GACCGGATCC GCGGGGTCAT CGACGTGGGC AACTCCCTCA TTGACCGTGG GGCCTGTGCC 
GGCAGCGAGG ATGCCGTCAA GGCCCGGCTG GCCGCCTTAG CTGACCAGTG GCAGTTCCTG 
GTGCAGAAGT CGGCTGAGAA GAGCCAGAAG CTGAAAGAAG CCAACAAACA GCAGAACTTC 
AACACTGGCA TCAAGGACTT CGACTTCTGG CTCTCTGAGG TGGAAGCCCT GCTGGCCTCT 
GAGGATTATG GCAAAGACCT GGCTTCTGTG AACAACTTGC TAAAAAAACA TCAGCTGCTG 
GAGGCAGATA TCTCTGCCCA CGAGGACCGC CTGAAGGACC TCAACAGCCA GGCGGACAGC 
CTGATGACCA GCAGCGCCTT CGACACCTCC CAGGTGAAGG ATAAGCGGGA CACCATCAAC 
AGCCGTTTCC AGAAGATCAA GAGCATGGCC GCCTCCCGGC GAGCTAGGCT GAATGAGTCC 
CACCGCCTGC ACCAGTTCTT CCGGGACATG GACGATGAGG AGTCCTGGAT CAAGGAGAAG 
AAGCTGCTGG TGAGCTCAGA GGACTACGGC CGCGACCTGA CGGGCGTCCA GAACCTGAGG 
AAGAAGCACA AACGGCTGGA GGCAGAGCTG GCTGCCCACG AGCCGGCCAT TCAGGGTGTC 
CTGGACACTG GAAAGAAGCT GTCTGATGAC AACACCATCG GGAAGGAGGA GATTCAGCAG 
CGCCTGGCGC AGTTTGTGGA GCACTGGAAG GAGCTGAAGC AGCTGGCAGC TGCTCGGGGC 
CAGCGGCTAG AAGAATCTTT GGAATATCAG CAGTTTGTGG CCAACGTGGA GGAGGAGGAA 
GCCTGGATCA ATGAGAAGAT GACCCTGGTG GCCAGTGAGG ATTACGGAGA CACTCTTGCT 
GCCATCCAGG GCTTGCTGAA GAAACACGAA GCCTTCGAGA CGGACTTCAC TGTCCACAAG 
GACCGGGTGA ATGACGTCTG CACTAACGGA CAAGACCTGG TTAAGAAGAA CAATCACCAT 
GAGGAGAACA TCTCTTCCAA GATGAAGGGC TTGAACGGGA AAGTGTCAGA CTTGGAGAAG 
GCTGCCTCCC AGAGGAAGGC AAAGCTCGAC GAGAACTCGG CCTTCCTCCA GTTCAACTGG 
AAGGCCGATG TGGTGGAGTC CTGGATTGGT GAAAAGGAGA ACAGCTTGAA GACAGATGAT 
TATGGTCGAG ATCTGTCTTC TGTCCAAACT CTCCTCACCA AACAGGAAAC CTTCGATGCT 
GGCCTGCAGG CCTTCCAGCA GGAGGGCATC GCCAACATCA CTGCCCTCAA AGATCAGCTG 
CTGGCCGCCA AGCATGTTCA GTCCAAGGCC ATCGAGGCCC GCCACGCTGC CCTCATGAAG 
AGATGGAGCC AGCTCCTGGC CAATTCGGCC ACCCGGAAGA AGAAGCTGCT GGAGGCACAG 
AGTCACTTCC GCAAGGTGGA GGATCTCTTC CTGACCTTTG CCAAAAAGGC TTCAGCCTTC 
AACAGCTGGT TTGAAAATGC AGAGGAGGAC TTAACAGACC CAGTGCGCTG CAACTCCCTG 
GAAGAGATCA AGGCCTTGAG AGAGGCCCAC GATGCCTTCC GCTCCTCGCT GAGCTCAGCC 
CAGGCCGACT TCAACCAGCT GGCTGAGCTG GACCGCCAGA TCAAGAGCTT CCGCGTGGCC 
TCCAACCCCT ACACCTGGTT CACCATGGAG GCCCTGGAGG AGACCTGGAG GAACCTTCAG 
AAGATCATCA AGGAAAGGGA GCTGGAGCTG CAGAAGGAGC AGCGGCGGCA GGAGGAGAAC 
GACAAGCTGC GCCAGGAGTT CGCCCAGCAT GCCAACGCCT TCCACCAGTG GATCCAGGAG 
ACCAGAACGT ATCTCCTCGA TGGGTCCTGC ATGGTGGAAG AATCTGGGAC CCTTGAATCG 
CAGCTTGAAG CTACCAAACG CAAACACCAG GAGATTCGAG CCATGAGAAG TCAGCTCAAG 
AAGATTGAAG ACCTGGGGGC GGCCATGGAG GAAGCGCTGA TCCTGGACAA CAAGTACACG 
GAGCACAGCA CCGTGGGCCT GGCCCAGCAG TGGGACCAGC TCGACCAGCT GGGCATGCGG 
ATGCAGCACA ACCTGGAGCA GCAGATCCAG GCCAGGAACA CGACAGGAGT GACCGAGGAG 
GCCCTCAAAG AATTCAGCAT GATGTTCAAA CACTTCGACA AGGACAAGTC CGGCCGGCTG 
AATCACCAGG AGTTCAAATC CTGTCTGCGC TCCCTGGGCT ATGACCTGCC CATGGTGGAG 
GAAGGGGAGC CCGACCCTGA GTTCGAGGCC ATCTTGGATA CTGTGGATCC TAACAGGGAC 
GGCCACGTCT CCCTGCAAGA GTACATGGCC TTTATGATCA GCCGTGAGAC CGAGAATGTC 
AAGTCCAGCG AGGAGATTGA GAGCGCCTTC CGGGCCCTGA GCTCCGAAGG GAAGCCTTAT 
GTGACCAAGG AGGAGCTGTA CCAGAACCTG ACTCGGGAAC AAGCCGACTA CTGCGTCTCC 
CACATGAAGC CCTACGTGGA CGGCAAGGGC CGCGAACTCC CCACTGCTTT CGACTACGTT 
GAGTTCACCC GCTCGCTCTT CGTGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTAN1 ( XM_027966363.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_027822164.1 Ovis aries spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X10, mRNA.

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CloneID	OSm76657
Clone ID Related Accession (Same CDS sequence)	XM_027966363.1
Accession Version	XM_027966363.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7395bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-05
Organism	Ovis aries(sheep)
Product	spectrin alpha chain, non-erythrocytic 1 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040254.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ovis aries Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTCAT CGTTTCGTAA ACGCAGAGTC CAGAAAATGG ACCCAAGCGG GGTCAAAGTG 
CTGGAGACAG CCGAGGACAT CCAGGAGAGG CGGCAGCAGG TCCTGGACAG ATACCACCGC 
TTCAAGGAGC TCTCGACCCT GAGGCGCCAG AAGCTTGAAG ACTCCTACCG ATTCCAGTTT 
TTCCAAAGAG ATGCTGAGGA GCTGGAGAAA TGGATTCAGG AGAAGCTTCA GATTGCCTCG 
GATGAGAATT ACAAAGACCC AACCAACCTG CAGGGCAAGC TGCAGAAGCA TCAAGCCTTC 
GAAGCCGAGG TGCAGGCCAA CTCGGGTGCC ATCGTGAAGC TTGATGAGAC CGGTAACCTG 
ATGATCTCAG AAGGGCACTT CGCGTCCGAA ACCATCCGGA CTCGTCTGAT GGAGCTGCAC 
CGCCAGTGGG AATTGCTTCT GGAGAAGATG CGGGAGAAAG GCATCAAGCT GCTGCAGGCC 
CAGAAGCTGG TGCAGTACCT GCGGGAGTGT GAGGACGTGA TGGACTGGAT CAATGACAAG 
GAAGCCATTG TGACATCTGA GGAGCTGGGC CAGGACCTGG AGCACGTGGA GGTGTTACAG 
AAGAAATTCG AAGAGTTTCA GACAGACATG GCTGCCCACG AAGAGAGAGT CAATGAAGTG 
AACCAGTTTG CTGCCAAGCT CATTCAGGAG CAGCACCCTG AGGAGGAGCT GATCAAGACA 
AAGGAGGACG AGGTGAATGC AGCCTGGCAG CGGCTGAAGG GCCTGGCCCT CCAGCGGCAG 
GGGAAGCTGT TCGGGGCAGC TGAGGTCCAA CGCTTTAACA GGGATGTGGA CGAGACCATT 
AGCTGGATTA AGGAGAAGGA ACAGTTAATG GCCTCTGATG ACTTTGGCCG GGACCTGGCC 
AGTGTCCAGG CTCTACTGCG GAAACACGAG GGTTTGGAGA GAGATCTTGC TGCCTTGGAG 
GACAAGGTCA AAGCCCTGTG TGCCGAGGCT GACCGCCTGC AGCAGTCCCA CCCTCTGAGT 
GCCACCCAGA TCCAAGTGAA GCGAGAGGAG CTGATCACCA ACTGGGAGCA GATCCGCACC 
CTGGCCGCGG AGAGACACGC ACGTCTCAAC GATTCCTACA GGCTTCAGCG CTTCCTTGCT 
GACTTCCGAG ACCTCACCAG CTGGGTCACT GAGATGAAAG CCCTCATCAA TGCAGATGAA 
CTTGCCAATG ATGTGGCCGG GGCCGAAGCC CTGCTGGATA GGCATCAGGA GCACAAGGGT 
GAAATTGACG CTCATGAAGA TAGCTTTAAA TCTGCAGATG AGTCTGGACA GGCCCTGCTT 
GCAGCTGGTC ACTATGCCTC AGATGAAGTG AGGGAGAAGC TGACCATCCT GTCCGAGGAG 
CGGGCCGCGC TACTAGAGCT CTGGGAGCTG CGACGGCAGC AATATGAGCA GTGCATGGAC 
CTGCAGCTCT TCTACCGCGA CACGGAGCAG GTGGACAACT GGATGAGCAA ACAGGAGGCG 
TTCCTGTTGA ACGAAGACCT GGGTGATTCC TTGGATAGTG TGGAAGCGCT TCTCAAGAAG 
CACGAAGACT TTGAGAAGTC CCTCAGCGCC CAGGAGGAGA AGATCACAGC CTTAGATGAG 
TTTGCCACCA AGCTGATTCA GAACAACCAC TACGCGATGG AAGATGTGGC CACCCGGCGA 
GACGCACTGC TGAGCCGCCG CAACGCCCTC CATGAGAGAG CCATGTACCG CCGTGCCCAG 
CTTGCAGATT CCTTCCACTT GCAGCAGTTT TTCCGTGACT CTGATGAGCT CAAGAGTTGG 
GTCAACGAGA AGATGAAAAC AGCCACAGAT GAAGCTTATA AAGACCCATC CAACCTACAA 
GGGAAAGTGC AGAAGCACCA GGCCTTTGAG GCCGAGCTCT CAGCAAACCA GAGCCGCATC 
GACGCCTTGG AGAAAGCTGG GCAGAAGCTG ATCGACGTTG ACCACTATGC CAAGGATGAG 
GTGGCGACTC GGATGAATGA AGTTATCAGT CTGTGGAAGA AGCTGCTGGA GGCCACGGAG 
CTGAAAGGCA TAAAGCTCCG TGAGGCTAAC CAGCAGCAGC AGTTTAACCG CAACGTTGAG 
GACATCGAGC TGTGGCTGTA CGAAGTGGAA GGTCATCTGG CCTCGGATGA CTACGGCAAA 
GACCTCACCA ACGTCCAGAA CCTCCAGAAA AAGCATGCCC TGCTGGAGGC TGACGTGGCC 
GCTCACCAGG ATCGCATCGA TGGCATCACT ATCCAAGCCC GCCAGTTCCA GGATGCGGGC 
CACTTTGATG CTGAAAACAT CAAGAAGAAG CAGGAGGCCC TGGTAGCTCG CTACGAGGCA 
CTCAAGGAGC CCATGGTTGC CCGGAAGCAG AAGCTGGCCG ATTCCCTGCG GTTGCAGCAG 
CTCTTCCGTG ACGTTGAGGA TGAGGAGACG TGGATTCGGG AGAAAGAACC TATTGCCGCC 
TCCACCAACA GAGGCAAAGA TTTAATTGGA GTCCAGAACC TGCTAAAGAA ACACCAAGCG 
CTACAAGCAG AAATTGCTGG CCACGAGCCC CGCATCAAAG CAGTCACACA GAAGGGGAAC 
GCCATGGTAG AGGAGGGCCA TTTTGCTGCC GAGGACGTGA AGGCCAAGCT CCATGAACTG 
AACCAGAAGT GGGAGTCGCT GAAGTCCAAG GCCTCCCAGC GACGGCAGGA CCTGGAGGAC 
TCGCTGCAGG CCCAGCAGTA CTTCGCTGAT GCCAACGAGG CCGAGTCCTG GATGCGGGAG 
AAGGAGCCCA TCGTGGGCAG CACCGACTAT GGCAAAGATG AGGACTCTGC CGAGGCTCTG 
CTAAAGAAGC ACGAGGCATT GATGTCGGAT CTCAGCGCCT ATGGCAGCAG CATCCAGGCC 
CTGCGAGAGC AGGCCCAGTC GTGCCGGCAA CAAGTGGCCC CCATGGATGA CGAGACTGGG 
AAGGAGCTGG TCTTGGCGCT CTATGACTAT CAGGAGAAGA GTCCCCGAGA GGTCACCATG 
AAGAAAGGAG ACATTCTCAC CTTACTCAAC AGCACCAACA AGGACTGGTG GAAAGTGGAA 
GTGAACGACC GGCAGGGCTT CGTGCCGGCC GCCTACGTGA AGAAGCTGGA CCCAGCGCAG 
TCGGCCTCCC GGGAGAACCT CCTGGAAGAG CAGGGCAGCA TCGCGCTGCG GCAGGAGCAG 
ATTGACAACC AGTACCATTC TCTGCTGGAA CTGGGAGAGA AGCGTAAAGG CATGTTGGAG 
AAGAGCTGTA AGAAGTTCAT GCTGTTCCGT GAAGCGAACG AGCTGCAGCA GTGGATCAAC 
GAGAAGGAAG CGGCCCTGAC AAGCGAGGAG GTCGGCGCGG ACTTGGAGCA GGTCGAAGTG 
CTGCAGAAGA AGTTTGACGA CTTCCAGAAG GATCTCAAGG CCAATGAGTC ACGGCTGAAG 
GACATTAACA AGGTGGCTGA AGACCTGGAG TCGGAAGGCC TGATGGCGGA GGAGGTGCAG 
GCCGTGCAGC AGCAGGAGGT GTATGGCGCG ATGCCCAGGG ATGAATCTGA TTCCAAGACA 
GCCTCCCCGT GGAAGTCTGC TCGCCTGATG GTTCACACCG TGGCCACCTT TAATTCCATC 
AAGGAGCTCA ATGAGCGCTG GCGGTCCCTG CAGCAGCTGG CTGAGGAGCG GAGCCAACTC 
TTGGGCAGCG CCCACGAAGT GCAGCGGTTC CACAGAGATG CTGACGAAAC CAAAGAGTGG 
ATCGAGGAGA AGAACCAGGC TCTAAACACA GACAACTATG GCCACGACCT GGCCAGCGTC 
CAGGCCCTGC AGCGCAAGCA TGAGGGCTTC GAGAGGGACC TCGCGGCCCT TGGCGACAAG 
GTGAACTCGC TCGGGGAGAC AGCCGAGCGC CTGATCCAGT CCCACCCCGA GTCGGCAGAG 
GACCTGCAGG AGAAGTGCAC TGAGCTGAAC CAGGCATGGA GCAGCCTGGG CAAGCGTGCC 
GACCAGCGCA AGGCCAAGCT GGGCGACTCC CACGACCTGC AGCGCTTCCT CAGCGACTTC 
CGGGACCTCA TGTCTTGGAT CAATGGAATC CGAGGTCTGG TGTCCTCAGA CGAGCTAGCC 
AAGGATGTCA CTGGAGCTGA GGCCCTGCTG GAGCGACACC AGGAACACCG GACAGAAATT 
GATGCCAGAG CTGGCACTTT CCAGGCATTC GAGCAGTTTG GGCAGCAGCT GTTGGCTCAT 
GGGCACTACG CCAGCCCCGA GATCAAGGAG AAGCTCGATG TTCTAGACCA GGAGCGCGCA 
GACCTCGAGA AGGCCTGGGT CCAGCGCAGG ATGATGCTGG ATCAGTGCCT TGAATTGCAG 
CTGTTCCATC GGGATTGCGA GCAGGCCGAG AACTGGATGG CTGCCCGGGA GGCCTTCCTG 
AACACAGAAG ACAAAGGGGA CTCGCTAGAC AGCGTGGAGG CTCTGATCAA AAAACACGAG 
GACTTTGACA AAGCAATTAA TGTCCAGGAG GAGAAGATTG CTGCCCTGCA GTCCTTTGCT 
GACCAGCTCA TTGCTGGCGG TCACTACGCC AAAGGCGACA TCTCCAGCCG GCGCAATGAG 
GTTTTGGACA GGTGGCGCCG TCTAAAAGCT CAGATGATTG AGAAGAGGTC AAAGCTGGGA 
GAATCGCAGA CCCTCCAGCA GTTCAGCCGG GATGTAGACG AGATTGAAGC TTGGATCAGT 
GAAAAATTAC AGACTGCAAG TGATGAGTCA TATAAGGACC CCACCAACAT CCAGAGCAAG 
CATCAGAAGC ACCAGGCCTT CGAGGCAGAA CTGCACGCCA ATGCGGACCG GATCCGCGGG 
GTCATCGACG TGGGCAACTC CCTCATTGAC CGTGGGGCCT GTGCCGGCAG CGAGGATGCC 
GTCAAGGCCC GGCTGGCCGC CTTAGCTGAC CAGTGGCAGT TCCTGGTGCA GAAGTCGGCT 
GAGAAGAGCC AGAAGCTGAA AGAAGCCAAC AAACAGCAGA ACTTCAACAC TGGCATCAAG 
GACTTCGACT TCTGGCTCTC TGAGGTGGAA GCCCTGCTGG CCTCTGAGGA TTATGGCAAA 
GACCTGGCTT CTGTGAACAA CTTGCTAAAA AAACATCAGC TGCTGGAGGC AGATATCTCT 
GCCCACGAGG ACCGCCTGAA GGACCTCAAC AGCCAGGCGG ACAGCCTGAT GACCAGCAGC 
GCCTTCGACA CCTCCCAGGT GAAGGATAAG CGGGACACCA TCAACAGCCG TTTCCAGAAG 
ATCAAGAGCA TGGCCGCCTC CCGGCGAGCT AGGCTGAATG AGTCCCACCG CCTGCACCAG 
TTCTTCCGGG ACATGGACGA TGAGGAGTCC TGGATCAAGG AGAAGAAGCT GCTGGTGAGC 
TCAGAGGACT ACGGCCGCGA CCTGACGGGC GTCCAGAACC TGAGGAAGAA GCACAAACGG 
CTGGAGGCAG AGCTGGCTGC CCACGAGCCG GCCATTCAGG GTGTCCTGGA CACTGGAAAG 
AAGCTGTCTG ATGACAACAC CATCGGGAAG GAGGAGATTC AGCAGCGCCT GGCGCAGTTT 
GTGGAGCACT GGAAGGAGCT GAAGCAGCTG GCAGCTGCTC GGGGCCAGCG GCTAGAAGAA 
TCTTTGGAAT ATCAGCAGTT TGTGGCCAAC GTGGAGGAGG AGGAAGCCTG GATCAATGAG 
AAGATGACCC TGGTGGCCAG TGAGGATTAC GGAGACACTC TTGCTGCCAT CCAGGGCTTG 
CTGAAGAAAC ACGAAGCCTT CGAGACGGAC TTCACTGTCC ACAAGGACCG GGTGAATGAC 
GTCTGCACTA ACGGACAAGA CCTGGTTAAG AAGAACAATC ACCATGAGGA GAACATCTCT 
TCCAAGATGA AGGGCTTGAA CGGGAAAGTG TCAGACTTGG AGAAGGCTGC CTCCCAGAGG 
AAGGCAAAGC TCGACGAGAA CTCGGCCTTC CTCCAGTTCA ACTGGAAGGC CGATGTGGTG 
GAGTCCTGGA TTGGTGAAAA GGAGAACAGC TTGAAGACAG ATGATTATGG TCGAGATCTG 
TCTTCTGTCC AAACTCTCCT CACCAAACAG GAAACCTTCG ATGCTGGCCT GCAGGCCTTC 
CAGCAGGAGG GCATCGCCAA CATCACTGCC CTCAAAGATC AGCTGCTGGC CGCCAAGCAT 
GTTCAGTCCA AGGCCATCGA GGCCCGCCAC GCTGCCCTCA TGAAGAGATG GAGCCAGCTC 
CTGGCCAATT CGGCCACCCG GAAGAAGAAG CTGCTGGAGG CACAGAGTCA CTTCCGCAAG 
GTGGAGGATC TCTTCCTGAC CTTTGCCAAA AAGGCTTCAG CCTTCAACAG CTGGTTTGAA 
AATGCAGAGG AGGACTTAAC AGACCCAGTG CGCTGCAACT CCCTGGAAGA GATCAAGGCC 
TTGAGAGAGG CCCACGATGC CTTCCGCTCC TCGCTGAGCT CAGCCCAGGC CGACTTCAAC 
CAGCTGGCTG AGCTGGACCG CCAGATCAAG AGCTTCCGCG TGGCCTCCAA CCCCTACACC 
TGGTTCACCA TGGAGGCCCT GGAGGAGACC TGGAGGAACC TTCAGAAGAT CATCAAGGAA 
AGGGAGCTGG AGCTGCAGAA GGAGCAGCGG CGGCAGGAGG AGAACGACAA GCTGCGCCAG 
GAGTTCGCCC AGCATGCCAA CGCCTTCCAC CAGTGGATCC AGGAGACCAG AACGTATCTC 
CTCGATGGGT CCTGCATGGT GGAAGAATCT GGGACCCTTG AATCGCAGCT TGAAGCTACC 
AAACGCAAAC ACCAGGAGAT TCGAGCCATG AGAAGTCAGC TCAAGAAGAT TGAAGACCTG 
GGGGCGGCCA TGGAGGAAGC GCTGATCCTG GACAACAAGT ACACGGAGCA CAGCACCGTG 
GGCCTGGCCC AGCAGTGGGA CCAGCTCGAC CAGCTGGGCA TGCGGATGCA GCACAACCTG 
GAGCAGCAGA TCCAGGCCAG GAACACGACA GGAGTGACCG AGGAGGCCCT CAAAGAATTC 
AGCATGATGT TCAAACACTT CGACAAGGAC AAGTCCGGCC GGCTGAATCA CCAGGAGTTC 
AAATCCTGTC TGCGCTCCCT GGGCTATGAC CTGCCCATGG TGGAGGAAGG GGAGCCCGAC 
CCTGAGTTCG AGGCCATCTT GGATACTGTG GATCCTAACA GGGACGGCCA CGTCTCCCTG 
CAAGAGTACA TGGCCTTTAT GATCAGCCGT GAGACCGAGA ATGTCAAGTC CAGCGAGGAG 
ATTGAGAGCG CCTTCCGGGC CCTGAGCTCC GAAGGGAAGC CTTATGTGAC CAAGGAGGAG 
CTGTACCAGA ACCTGACTCG GGAACAAGCC GACTACTGCG TCTCCCACAT GAAGCCCTAC 
GTGGACGGCA AGGGCCGCGA ACTCCCCACT GCTTTCGACT ACGTTGAGTT CACCCGCTCG 
CTCTTCGTGA ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_027822164.1
CDS	70..7464
Translation	MLSSFRKRRVQKMDPSGVKVLETAEDIQERRQQVLDRYHRFKELSTLRRQKLEDSYRFQFFQRDAEELEKWIQEKLQIASDENYKDPTNLQGKLQKHQAFEAEVQANSGAIVKLDETGNLMISEGHFASETIRTRLMELHRQWELLLEKMREKGIKLLQAQKLVQYLRECEDVMDWINDKEAIVTSEELGQDLEHVEVLQKKFEEFQTDMAAHEERVNEVNQFAAKLIQEQHPEEELIKTKEDEVNAAWQRLKGLALQRQGKLFGAAEVQRFNRDVDETISWIKEKEQLMASDDFGRDLASVQALLRKHEGLERDLAALEDKVKALCAEADRLQQSHPLSATQIQVKREELITNWEQIRTLAAERHARLNDSYRLQRFLADFRDLTSWVTEMKALINADELANDVAGAEALLDRHQEHKGEIDAHEDSFKSADESGQALLAAGHYASDEVREKLTILSEERAALLELWELRRQQYEQCMDLQLFYRDTEQVDNWMSKQEAFLLNEDLGDSLDSVEALLKKHEDFEKSLSAQEEKITALDEFATKLIQNNHYAMEDVATRRDALLSRRNALHERAMYRRAQLADSFHLQQFFRDSDELKSWVNEKMKTATDEAYKDPSNLQGKVQKHQAFEAELSANQSRIDALEKAGQKLIDVDHYAKDEVATRMNEVISLWKKLLEATELKGIKLREANQQQQFNRNVEDIELWLYEVEGHLASDDYGKDLTNVQNLQKKHALLEADVAAHQDRIDGITIQARQFQDAGHFDAENIKKKQEALVARYEALKEPMVARKQKLADSLRLQQLFRDVEDEETWIREKEPIAASTNRGKDLIGVQNLLKKHQALQAEIAGHEPRIKAVTQKGNAMVEEGHFAAEDVKAKLHELNQKWESLKSKASQRRQDLEDSLQAQQYFADANEAESWMREKEPIVGSTDYGKDEDSAEALLKKHEALMSDLSAYGSSIQALREQAQSCRQQVAPMDDETGKELVLALYDYQEKSPREVTMKKGDILTLLNSTNKDWWKVEVNDRQGFVPAAYVKKLDPAQSASRENLLEEQGSIALRQEQIDNQYHSLLELGEKRKGMLEKSCKKFMLFREANELQQWINEKEAALTSEEVGADLEQVEVLQKKFDDFQKDLKANESRLKDINKVAEDLESEGLMAEEVQAVQQQEVYGAMPRDESDSKTASPWKSARLMVHTVATFNSIKELNERWRSLQQLAEERSQLLGSAHEVQRFHRDADETKEWIEEKNQALNTDNYGHDLASVQALQRKHEGFERDLAALGDKVNSLGETAERLIQSHPESAEDLQEKCTELNQAWSSLGKRADQRKAKLGDSHDLQRFLSDFRDLMSWINGIRGLVSSDELAKDVTGAEALLERHQEHRTEIDARAGTFQAFEQFGQQLLAHGHYASPEIKEKLDVLDQERADLEKAWVQRRMMLDQCLELQLFHRDCEQAENWMAAREAFLNTEDKGDSLDSVEALIKKHEDFDKAINVQEEKIAALQSFADQLIAGGHYAKGDISSRRNEVLDRWRRLKAQMIEKRSKLGESQTLQQFSRDVDEIEAWISEKLQTASDESYKDPTNIQSKHQKHQAFEAELHANADRIRGVIDVGNSLIDRGACAGSEDAVKARLAALADQWQFLVQKSAEKSQKLKEANKQQNFNTGIKDFDFWLSEVEALLASEDYGKDLASVNNLLKKHQLLEADISAHEDRLKDLNSQADSLMTSSAFDTSQVKDKRDTINSRFQKIKSMAASRRARLNESHRLHQFFRDMDDEESWIKEKKLLVSSEDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNTIGKEEIQQRLAQFVEHWKELKQLAAARGQRLEESLEYQQFVANVEEEEAWINEKMTLVASEDYGDTLAAIQGLLKKHEAFETDFTVHKDRVNDVCTNGQDLVKKNNHHEENISSKMKGLNGKVSDLEKAASQRKAKLDENSAFLQFNWKADVVESWIGEKENSLKTDDYGRDLSSVQTLLTKQETFDAGLQAFQQEGIANITALKDQLLAAKHVQSKAIEARHAALMKRWSQLLANSATRKKKLLEAQSHFRKVEDLFLTFAKKASAFNSWFENAEEDLTDPVRCNSLEEIKALREAHDAFRSSLSSAQADFNQLAELDRQIKSFRVASNPYTWFTMEALEETWRNLQKIIKERELELQKEQRRQEENDKLRQEFAQHANAFHQWIQETRTYLLDGSCMVEESGTLESQLEATKRKHQEIRAMRSQLKKIEDLGAAMEEALILDNKYTEHSTVGLAQQWDQLDQLGMRMQHNLEQQIQARNTTGVTEEALKEFSMMFKHFDKDKSGRLNHQEFKSCLRSLGYDLPMVEEGEPDPEFEAILDTVDPNRDGHVSLQEYMAFMISRETENVKSSEEIESAFRALSSEGKPYVTKEELYQNLTREQADYCVSHMKPYVDGKGRELPTAFDYVEFTRSLFVN

Target ORF information:

RefSeq Version	XM_027966363.1
Organism	Ovis aries(sheep)
Definition	Ovis aries spectrin alpha, non-erythrocytic 1 (SPTAN1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027966363.1

ORF Insert Sequence:

ATGCTGTCAT CGTTTCGTAA ACGCAGAGTC CAGAAAATGG ACCCAAGCGG GGTCAAAGTG 
CTGGAGACAG CCGAGGACAT CCAGGAGAGG CGGCAGCAGG TCCTGGACAG ATACCACCGC 
TTCAAGGAGC TCTCGACCCT GAGGCGCCAG AAGCTTGAAG ACTCCTACCG ATTCCAGTTT 
TTCCAAAGAG ATGCTGAGGA GCTGGAGAAA TGGATTCAGG AGAAGCTTCA GATTGCCTCG 
GATGAGAATT ACAAAGACCC AACCAACCTG CAGGGCAAGC TGCAGAAGCA TCAAGCCTTC 
GAAGCCGAGG TGCAGGCCAA CTCGGGTGCC ATCGTGAAGC TTGATGAGAC CGGTAACCTG 
ATGATCTCAG AAGGGCACTT CGCGTCCGAA ACCATCCGGA CTCGTCTGAT GGAGCTGCAC 
CGCCAGTGGG AATTGCTTCT GGAGAAGATG CGGGAGAAAG GCATCAAGCT GCTGCAGGCC 
CAGAAGCTGG TGCAGTACCT GCGGGAGTGT GAGGACGTGA TGGACTGGAT CAATGACAAG 
GAAGCCATTG TGACATCTGA GGAGCTGGGC CAGGACCTGG AGCACGTGGA GGTGTTACAG 
AAGAAATTCG AAGAGTTTCA GACAGACATG GCTGCCCACG AAGAGAGAGT CAATGAAGTG 
AACCAGTTTG CTGCCAAGCT CATTCAGGAG CAGCACCCTG AGGAGGAGCT GATCAAGACA 
AAGGAGGACG AGGTGAATGC AGCCTGGCAG CGGCTGAAGG GCCTGGCCCT CCAGCGGCAG 
GGGAAGCTGT TCGGGGCAGC TGAGGTCCAA CGCTTTAACA GGGATGTGGA CGAGACCATT 
AGCTGGATTA AGGAGAAGGA ACAGTTAATG GCCTCTGATG ACTTTGGCCG GGACCTGGCC 
AGTGTCCAGG CTCTACTGCG GAAACACGAG GGTTTGGAGA GAGATCTTGC TGCCTTGGAG 
GACAAGGTCA AAGCCCTGTG TGCCGAGGCT GACCGCCTGC AGCAGTCCCA CCCTCTGAGT 
GCCACCCAGA TCCAAGTGAA GCGAGAGGAG CTGATCACCA ACTGGGAGCA GATCCGCACC 
CTGGCCGCGG AGAGACACGC ACGTCTCAAC GATTCCTACA GGCTTCAGCG CTTCCTTGCT 
GACTTCCGAG ACCTCACCAG CTGGGTCACT GAGATGAAAG CCCTCATCAA TGCAGATGAA 
CTTGCCAATG ATGTGGCCGG GGCCGAAGCC CTGCTGGATA GGCATCAGGA GCACAAGGGT 
GAAATTGACG CTCATGAAGA TAGCTTTAAA TCTGCAGATG AGTCTGGACA GGCCCTGCTT 
GCAGCTGGTC ACTATGCCTC AGATGAAGTG AGGGAGAAGC TGACCATCCT GTCCGAGGAG 
CGGGCCGCGC TACTAGAGCT CTGGGAGCTG CGACGGCAGC AATATGAGCA GTGCATGGAC 
CTGCAGCTCT TCTACCGCGA CACGGAGCAG GTGGACAACT GGATGAGCAA ACAGGAGGCG 
TTCCTGTTGA ACGAAGACCT GGGTGATTCC TTGGATAGTG TGGAAGCGCT TCTCAAGAAG 
CACGAAGACT TTGAGAAGTC CCTCAGCGCC CAGGAGGAGA AGATCACAGC CTTAGATGAG 
TTTGCCACCA AGCTGATTCA GAACAACCAC TACGCGATGG AAGATGTGGC CACCCGGCGA 
GACGCACTGC TGAGCCGCCG CAACGCCCTC CATGAGAGAG CCATGTACCG CCGTGCCCAG 
CTTGCAGATT CCTTCCACTT GCAGCAGTTT TTCCGTGACT CTGATGAGCT CAAGAGTTGG 
GTCAACGAGA AGATGAAAAC AGCCACAGAT GAAGCTTATA AAGACCCATC CAACCTACAA 
GGGAAAGTGC AGAAGCACCA GGCCTTTGAG GCCGAGCTCT CAGCAAACCA GAGCCGCATC 
GACGCCTTGG AGAAAGCTGG GCAGAAGCTG ATCGACGTTG ACCACTATGC CAAGGATGAG 
GTGGCGACTC GGATGAATGA AGTTATCAGT CTGTGGAAGA AGCTGCTGGA GGCCACGGAG 
CTGAAAGGCA TAAAGCTCCG TGAGGCTAAC CAGCAGCAGC AGTTTAACCG CAACGTTGAG 
GACATCGAGC TGTGGCTGTA CGAAGTGGAA GGTCATCTGG CCTCGGATGA CTACGGCAAA 
GACCTCACCA ACGTCCAGAA CCTCCAGAAA AAGCATGCCC TGCTGGAGGC TGACGTGGCC 
GCTCACCAGG ATCGCATCGA TGGCATCACT ATCCAAGCCC GCCAGTTCCA GGATGCGGGC 
CACTTTGATG CTGAAAACAT CAAGAAGAAG CAGGAGGCCC TGGTAGCTCG CTACGAGGCA 
CTCAAGGAGC CCATGGTTGC CCGGAAGCAG AAGCTGGCCG ATTCCCTGCG GTTGCAGCAG 
CTCTTCCGTG ACGTTGAGGA TGAGGAGACG TGGATTCGGG AGAAAGAACC TATTGCCGCC 
TCCACCAACA GAGGCAAAGA TTTAATTGGA GTCCAGAACC TGCTAAAGAA ACACCAAGCG 
CTACAAGCAG AAATTGCTGG CCACGAGCCC CGCATCAAAG CAGTCACACA GAAGGGGAAC 
GCCATGGTAG AGGAGGGCCA TTTTGCTGCC GAGGACGTGA AGGCCAAGCT CCATGAACTG 
AACCAGAAGT GGGAGTCGCT GAAGTCCAAG GCCTCCCAGC GACGGCAGGA CCTGGAGGAC 
TCGCTGCAGG CCCAGCAGTA CTTCGCTGAT GCCAACGAGG CCGAGTCCTG GATGCGGGAG 
AAGGAGCCCA TCGTGGGCAG CACCGACTAT GGCAAAGATG AGGACTCTGC CGAGGCTCTG 
CTAAAGAAGC ACGAGGCATT GATGTCGGAT CTCAGCGCCT ATGGCAGCAG CATCCAGGCC 
CTGCGAGAGC AGGCCCAGTC GTGCCGGCAA CAAGTGGCCC CCATGGATGA CGAGACTGGG 
AAGGAGCTGG TCTTGGCGCT CTATGACTAT CAGGAGAAGA GTCCCCGAGA GGTCACCATG 
AAGAAAGGAG ACATTCTCAC CTTACTCAAC AGCACCAACA AGGACTGGTG GAAAGTGGAA 
GTGAACGACC GGCAGGGCTT CGTGCCGGCC GCCTACGTGA AGAAGCTGGA CCCAGCGCAG 
TCGGCCTCCC GGGAGAACCT CCTGGAAGAG CAGGGCAGCA TCGCGCTGCG GCAGGAGCAG 
ATTGACAACC AGTACCATTC TCTGCTGGAA CTGGGAGAGA AGCGTAAAGG CATGTTGGAG 
AAGAGCTGTA AGAAGTTCAT GCTGTTCCGT GAAGCGAACG AGCTGCAGCA GTGGATCAAC 
GAGAAGGAAG CGGCCCTGAC AAGCGAGGAG GTCGGCGCGG ACTTGGAGCA GGTCGAAGTG 
CTGCAGAAGA AGTTTGACGA CTTCCAGAAG GATCTCAAGG CCAATGAGTC ACGGCTGAAG 
GACATTAACA AGGTGGCTGA AGACCTGGAG TCGGAAGGCC TGATGGCGGA GGAGGTGCAG 
GCCGTGCAGC AGCAGGAGGT GTATGGCGCG ATGCCCAGGG ATGAATCTGA TTCCAAGACA 
GCCTCCCCGT GGAAGTCTGC TCGCCTGATG GTTCACACCG TGGCCACCTT TAATTCCATC 
AAGGAGCTCA ATGAGCGCTG GCGGTCCCTG CAGCAGCTGG CTGAGGAGCG GAGCCAACTC 
TTGGGCAGCG CCCACGAAGT GCAGCGGTTC CACAGAGATG CTGACGAAAC CAAAGAGTGG 
ATCGAGGAGA AGAACCAGGC TCTAAACACA GACAACTATG GCCACGACCT GGCCAGCGTC 
CAGGCCCTGC AGCGCAAGCA TGAGGGCTTC GAGAGGGACC TCGCGGCCCT TGGCGACAAG 
GTGAACTCGC TCGGGGAGAC AGCCGAGCGC CTGATCCAGT CCCACCCCGA GTCGGCAGAG 
GACCTGCAGG AGAAGTGCAC TGAGCTGAAC CAGGCATGGA GCAGCCTGGG CAAGCGTGCC 
GACCAGCGCA AGGCCAAGCT GGGCGACTCC CACGACCTGC AGCGCTTCCT CAGCGACTTC 
CGGGACCTCA TGTCTTGGAT CAATGGAATC CGAGGTCTGG TGTCCTCAGA CGAGCTAGCC 
AAGGATGTCA CTGGAGCTGA GGCCCTGCTG GAGCGACACC AGGAACACCG GACAGAAATT 
GATGCCAGAG CTGGCACTTT CCAGGCATTC GAGCAGTTTG GGCAGCAGCT GTTGGCTCAT 
GGGCACTACG CCAGCCCCGA GATCAAGGAG AAGCTCGATG TTCTAGACCA GGAGCGCGCA 
GACCTCGAGA AGGCCTGGGT CCAGCGCAGG ATGATGCTGG ATCAGTGCCT TGAATTGCAG 
CTGTTCCATC GGGATTGCGA GCAGGCCGAG AACTGGATGG CTGCCCGGGA GGCCTTCCTG 
AACACAGAAG ACAAAGGGGA CTCGCTAGAC AGCGTGGAGG CTCTGATCAA AAAACACGAG 
GACTTTGACA AAGCAATTAA TGTCCAGGAG GAGAAGATTG CTGCCCTGCA GTCCTTTGCT 
GACCAGCTCA TTGCTGGCGG TCACTACGCC AAAGGCGACA TCTCCAGCCG GCGCAATGAG 
GTTTTGGACA GGTGGCGCCG TCTAAAAGCT CAGATGATTG AGAAGAGGTC AAAGCTGGGA 
GAATCGCAGA CCCTCCAGCA GTTCAGCCGG GATGTAGACG AGATTGAAGC TTGGATCAGT 
GAAAAATTAC AGACTGCAAG TGATGAGTCA TATAAGGACC CCACCAACAT CCAGAGCAAG 
CATCAGAAGC ACCAGGCCTT CGAGGCAGAA CTGCACGCCA ATGCGGACCG GATCCGCGGG 
GTCATCGACG TGGGCAACTC CCTCATTGAC CGTGGGGCCT GTGCCGGCAG CGAGGATGCC 
GTCAAGGCCC GGCTGGCCGC CTTAGCTGAC CAGTGGCAGT TCCTGGTGCA GAAGTCGGCT 
GAGAAGAGCC AGAAGCTGAA AGAAGCCAAC AAACAGCAGA ACTTCAACAC TGGCATCAAG 
GACTTCGACT TCTGGCTCTC TGAGGTGGAA GCCCTGCTGG CCTCTGAGGA TTATGGCAAA 
GACCTGGCTT CTGTGAACAA CTTGCTAAAA AAACATCAGC TGCTGGAGGC AGATATCTCT 
GCCCACGAGG ACCGCCTGAA GGACCTCAAC AGCCAGGCGG ACAGCCTGAT GACCAGCAGC 
GCCTTCGACA CCTCCCAGGT GAAGGATAAG CGGGACACCA TCAACAGCCG TTTCCAGAAG 
ATCAAGAGCA TGGCCGCCTC CCGGCGAGCT AGGCTGAATG AGTCCCACCG CCTGCACCAG 
TTCTTCCGGG ACATGGACGA TGAGGAGTCC TGGATCAAGG AGAAGAAGCT GCTGGTGAGC 
TCAGAGGACT ACGGCCGCGA CCTGACGGGC GTCCAGAACC TGAGGAAGAA GCACAAACGG 
CTGGAGGCAG AGCTGGCTGC CCACGAGCCG GCCATTCAGG GTGTCCTGGA CACTGGAAAG 
AAGCTGTCTG ATGACAACAC CATCGGGAAG GAGGAGATTC AGCAGCGCCT GGCGCAGTTT 
GTGGAGCACT GGAAGGAGCT GAAGCAGCTG GCAGCTGCTC GGGGCCAGCG GCTAGAAGAA 
TCTTTGGAAT ATCAGCAGTT TGTGGCCAAC GTGGAGGAGG AGGAAGCCTG GATCAATGAG 
AAGATGACCC TGGTGGCCAG TGAGGATTAC GGAGACACTC TTGCTGCCAT CCAGGGCTTG 
CTGAAGAAAC ACGAAGCCTT CGAGACGGAC TTCACTGTCC ACAAGGACCG GGTGAATGAC 
GTCTGCACTA ACGGACAAGA CCTGGTTAAG AAGAACAATC ACCATGAGGA GAACATCTCT 
TCCAAGATGA AGGGCTTGAA CGGGAAAGTG TCAGACTTGG AGAAGGCTGC CTCCCAGAGG 
AAGGCAAAGC TCGACGAGAA CTCGGCCTTC CTCCAGTTCA ACTGGAAGGC CGATGTGGTG 
GAGTCCTGGA TTGGTGAAAA GGAGAACAGC TTGAAGACAG ATGATTATGG TCGAGATCTG 
TCTTCTGTCC AAACTCTCCT CACCAAACAG GAAACCTTCG ATGCTGGCCT GCAGGCCTTC 
CAGCAGGAGG GCATCGCCAA CATCACTGCC CTCAAAGATC AGCTGCTGGC CGCCAAGCAT 
GTTCAGTCCA AGGCCATCGA GGCCCGCCAC GCTGCCCTCA TGAAGAGATG GAGCCAGCTC 
CTGGCCAATT CGGCCACCCG GAAGAAGAAG CTGCTGGAGG CACAGAGTCA CTTCCGCAAG 
GTGGAGGATC TCTTCCTGAC CTTTGCCAAA AAGGCTTCAG CCTTCAACAG CTGGTTTGAA 
AATGCAGAGG AGGACTTAAC AGACCCAGTG CGCTGCAACT CCCTGGAAGA GATCAAGGCC 
TTGAGAGAGG CCCACGATGC CTTCCGCTCC TCGCTGAGCT CAGCCCAGGC CGACTTCAAC 
CAGCTGGCTG AGCTGGACCG CCAGATCAAG AGCTTCCGCG TGGCCTCCAA CCCCTACACC 
TGGTTCACCA TGGAGGCCCT GGAGGAGACC TGGAGGAACC TTCAGAAGAT CATCAAGGAA 
AGGGAGCTGG AGCTGCAGAA GGAGCAGCGG CGGCAGGAGG AGAACGACAA GCTGCGCCAG 
GAGTTCGCCC AGCATGCCAA CGCCTTCCAC CAGTGGATCC AGGAGACCAG AACGTATCTC 
CTCGATGGGT CCTGCATGGT GGAAGAATCT GGGACCCTTG AATCGCAGCT TGAAGCTACC 
AAACGCAAAC ACCAGGAGAT TCGAGCCATG AGAAGTCAGC TCAAGAAGAT TGAAGACCTG 
GGGGCGGCCA TGGAGGAAGC GCTGATCCTG GACAACAAGT ACACGGAGCA CAGCACCGTG 
GGCCTGGCCC AGCAGTGGGA CCAGCTCGAC CAGCTGGGCA TGCGGATGCA GCACAACCTG 
GAGCAGCAGA TCCAGGCCAG GAACACGACA GGAGTGACCG AGGAGGCCCT CAAAGAATTC 
AGCATGATGT TCAAACACTT CGACAAGGAC AAGTCCGGCC GGCTGAATCA CCAGGAGTTC 
AAATCCTGTC TGCGCTCCCT GGGCTATGAC CTGCCCATGG TGGAGGAAGG GGAGCCCGAC 
CCTGAGTTCG AGGCCATCTT GGATACTGTG GATCCTAACA GGGACGGCCA CGTCTCCCTG 
CAAGAGTACA TGGCCTTTAT GATCAGCCGT GAGACCGAGA ATGTCAAGTC CAGCGAGGAG 
ATTGAGAGCG CCTTCCGGGC CCTGAGCTCC GAAGGGAAGC CTTATGTGAC CAAGGAGGAG 
CTGTACCAGA ACCTGACTCG GGAACAAGCC GACTACTGCG TCTCCCACAT GAAGCCCTAC 
GTGGACGGCA AGGGCCGCGA ACTCCCCACT GCTTTCGACT ACGTTGAGTT CACCCGCTCG 
CTCTTCGTGA ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	SPTAN1
Entrez Gene ID	101107431
Full Name	spectrin alpha, non-erythrocytic 1
Gene Type	protein-coding
Organism	Ovis aries(sheep)

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

SPTAN1 cDNA ORF clone, Ovis aries(sheep)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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