MDC1 gene cDNA ORF clone, Ovis aries(sheep)

mRNA	Protein	Name
XM_012100992.2	XP_011956382.1	mediator of DNA damage checkpoint protein 1 isoform X8
XM_015090177.1	XP_014945663.1	mediator of DNA damage checkpoint protein 1 isoform X9
XM_012164410.2	XP_012019800.1	mediator of DNA damage checkpoint protein 1 isoform X14
XM_027958859.1	XP_027814660.1	LOW QUALITY PROTEIN: mediator of DNA damage checkpoint protein 1
XM_012100986.2	XP_011956376.1	mediator of DNA damage checkpoint protein 1 isoform X3
XM_012100991.2	XP_011956381.1	mediator of DNA damage checkpoint protein 1 isoform X7
XM_012164405.2	XP_012019795.1	mediator of DNA damage checkpoint protein 1 isoform X11
XM_012164407.2	XP_012019797.1	mediator of DNA damage checkpoint protein 1 isoform X11
XM_012164411.2	XP_012019801.1	mediator of DNA damage checkpoint protein 1 isoform X15
XM_012164403.2	XP_012019793.1	mediator of DNA damage checkpoint protein 1 isoform X10
XM_012164406.2	XP_012019796.1	mediator of DNA damage checkpoint protein 1 isoform X11
XM_012164408.2	XP_012019798.1	mediator of DNA damage checkpoint protein 1 isoform X12
XM_012100984.2	XP_011956374.1	mediator of DNA damage checkpoint protein 1 isoform X1
XM_012100985.2	XP_011956375.1	mediator of DNA damage checkpoint protein 1 isoform X2
XM_012100988.2	XP_011956378.1	mediator of DNA damage checkpoint protein 1 isoform X5
XM_012164401.2	XP_012019791.1	mediator of DNA damage checkpoint protein 1 isoform X9
XM_012164402.2	XP_012019792.1	mediator of DNA damage checkpoint protein 1 isoform X9
XM_012164409.2	XP_012019799.1	mediator of DNA damage checkpoint protein 1 isoform X13
XM_012164404.2	XP_012019794.1	mediator of DNA damage checkpoint protein 1 isoform X11
XM_012164412.2	XP_012019802.1	mediator of DNA damage checkpoint protein 1 isoform X16
XM_012100987.2	XP_011956377.1	mediator of DNA damage checkpoint protein 1 isoform X4
XM_012100990.2	XP_011956380.1	mediator of DNA damage checkpoint protein 1 isoform X6

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OSm71853	XM_027958859.1 Latest version!	Ovis aries mediator of DNA damage checkpoint 1 (MDC1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OOv35148	XM_015090177.1 Latest version!	Ovis aries musimon mediator of DNA damage checkpoint 1 (MDC1), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OOv35148	XM_012164401.2 Latest version!	Ovis aries musimon mediator of DNA damage checkpoint 1 (MDC1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OOv35148	XM_012164402.2 Latest version!	Ovis aries musimon mediator of DNA damage checkpoint 1 (MDC1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OOv35149	XM_012164403.2 Latest version!	Ovis aries musimon mediator of DNA damage checkpoint 1 (MDC1), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OOv35150	XM_012164406.2 Latest version!	Ovis aries musimon mediator of DNA damage checkpoint 1 (MDC1), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OOv35150	XM_012164405.2 Latest version!	Ovis aries musimon mediator of DNA damage checkpoint 1 (MDC1), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OOv35150	XM_012164407.2 Latest version!	Ovis aries musimon mediator of DNA damage checkpoint 1 (MDC1), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OOv35150	XM_012164404.2 Latest version!	Ovis aries musimon mediator of DNA damage checkpoint 1 (MDC1), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OOv35151	XM_012164408.2 Latest version!	Ovis aries musimon mediator of DNA damage checkpoint 1 (MDC1), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OOv35152	XM_012164409.2 Latest version!	Ovis aries musimon mediator of DNA damage checkpoint 1 (MDC1), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OOv35153	XM_012164410.2 Latest version!	Ovis aries musimon mediator of DNA damage checkpoint 1 (MDC1), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OOv35154	XM_012164411.2 Latest version!	Ovis aries musimon mediator of DNA damage checkpoint 1 (MDC1), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OOv35155	XM_012164412.2 Latest version!	Ovis aries musimon mediator of DNA damage checkpoint 1 (MDC1), transcript variant X21, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OSm28025	XM_012100984.2 Latest version!	Ovis aries mediator of DNA damage checkpoint 1 (MDC1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OSm28026	XM_012100985.2 Latest version!	Ovis aries mediator of DNA damage checkpoint 1 (MDC1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OSm28027	XM_012100986.2 Latest version!	Ovis aries mediator of DNA damage checkpoint 1 (MDC1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OSm28028	XM_012100987.2 Latest version!	Ovis aries mediator of DNA damage checkpoint 1 (MDC1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OSm28029	XM_012100988.2 Latest version!	Ovis aries mediator of DNA damage checkpoint 1 (MDC1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OSm28030	XM_012100990.2 Latest version!	Ovis aries mediator of DNA damage checkpoint 1 (MDC1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OSm28031	XM_012100991.2 Latest version!	Ovis aries mediator of DNA damage checkpoint 1 (MDC1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OSm28032	XM_012100992.2 Latest version!	Ovis aries mediator of DNA damage checkpoint 1 (MDC1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00

MDC1 ( XM_027958859.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_027814660.1 Ovis aries mediator of DNA damage checkpoint 1 (MDC1), mRNA.

Price & Availability↑

CloneID	OSm71853
Clone ID Related Accession (Same CDS sequence)	XM_027958859.1
Accession Version	XM_027958859.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5811bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-05
Organism	Ovis aries(sheep)
Product	LOW QUALITY PROTEIN: mediator of DNA damage checkpoint protein 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040271.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ovis aries Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 1 indel ##RefSeq-Attributes-END##

ATGGAGGTGT CCCACTTTCC TTGTCTACAT TTTCCCTTTA AGATCATGGA AGACACCCAG 
ATTTTAAACT GGGAAGTTGA AGAAGAGGAG GAGGTTGCAG AGAGCCCCAA TGAATCTCTG 
GGGTATAGCT TGGAGCCCCT AGCACAGCTG CATATCTTCA GTAGTTCCTA TGGACCAGAA 
AAAGACTTCC CACTCTACCT TGGAAAGAAC ATGATAGGCC GAATGCCTGA TTGCTCTGTG 
GCCCTGCCCT TTTCATCAAT ATCCAAACAA CATGCAGTGA TTGAAATCTC TGCCTGGGAC 
AAGGCGCCTG TCCTTCGGGA TTGCGGGAGC CTCAATGGCA CTCAAATCCT GCGGCCTCCT 
AAGGTCCTGA GCCCTGGGGT GAGTCATCGT TTGCGGGACC GGGAGTTGAT TCTCTTTGCT 
GACTTGCCCT GCCAGTACCA TCGCTTGGAT GTCCCCCGGC CTTTTGTCTC CCGGGGCCCT 
CTAAGTGTAG AGGAGACACC CAGGGTACAG GGAGGAACTC AACCCTCCAG GCTTCTGTTG 
GCTGAGGACT CAGAGGAAGA AGTAGATTCC CTTTTGGACA AGTGTGTGGT GAAAGGACCA 
AGGACCTCCT CTTCGGCAAC AGTCGTTCCA GAGAGTGATG AAGAAGGGCC TTCCCCTGCC 
CCAGATGGGC CCGGGCCACC TTCTGCCTTC AACTTGAACA GCGACACGGA TGAGGAAGAA 
AGTCAGGAAT CAGGAGCAGG GGAGGCCTCT TCAGCTGCCA GAAGAGGTAC CGCTGCAGAG 
ACGGAACAGC CTGAACCTGT CACAGCCGAG ATCCAGATTG AGAAGGATCA GTGTTCCGTG 
AAGGAGAGGA ACGGGGACAC AGAAATCGAG AGGGATGTGA GGAATGGGGT GGTTCCGACT 
GGAGTGATTC TGGAGAGGAG CCAGCCTTCT GGGGAGGACA GTGACACAGA TGTGAGTGAT 
GAGAGTGGGC CTCGAAGAAG GCTTGTTGGG GTCCGTCCGA AAAGGGCCTG GTCTTGTAAC 
TTCATAGACA GTGATACCGA TGGAGAGGAC GAGGGGATCC CTGCTACCCC AGCAGTGGTT 
CCCATGAAGG AGAGGCAGAT CTTCCACGAA GCTGGTACAC AGAGCCCCCA GGCACCTGGT 
GTGGCACGTT GGCAGGGGAG CCCAGCTGAT GGTGATACAG ATATAGAGGA GGGGGAGGCC 
CCCCCAGACA GAAGCCAAGC CTCCGTGGTG ATCGACAGCA ATACAGACGA TGAGGAAGAA 
GTCTCAGCAG CACTGACACT GGCATGTCTA AGAGAGAGCC AGGCTGGTAA GTGGAACCGA 
GATCCAGATG CAGAAGAGAC CAGGGCCCAA CCCGTGGCCC TTCTGGAGCG AAGCCAGGCC 
TCTGCTGGGG GAGACAGTGA CACAGATGTG GAGGAAGAGG GGCTCCCAGT GGAAAGGAGG 
GGAATGGTTC CCAAGGGTTA CATGGACAGG GAATATTCAA AAAAGAGCCA GCACCTTCCC 
AGGGACAGTG ATACAGAGGG GAAGGAAGAT GAGAGCTCAC CGGGGGTCCA CCTGGAGAGA 
AGCCAGGCCT CTGCACAAGT GGAGGATGAG GTCCCACTGG GGCCAGCTGT TGCACTTCCG 
GAGAAGCGTC AGGTACAAGG CATAGTGTGG CCACATCACA CCGATGCGGA GGCAGAAGGG 
GACCCGGCAC AGCTCCCCGT GCTGCGTCTA GAGGAAGCCC GGCCTCCTCT AGCTGGGGAC 
TGTGAACTGG ATTCGGAGAA CACATCTGCT GTCAGAAAGA GCCAGCTTCC GGCGGAAAAA 
GATGCTGGGA CCACGTGGGC TGCAGCCGTT CCTGAACAGG ACAGAGCACT TGCCATCGGG 
ACCCAGGGTG GGTCATCCAC GGCACCAGGG GAGCAAGACC TTCTCCCGGT CTCAAGGGAA 
GACCTAGCAG ATATGGTGGT GGACATAGGC ACTCCAGGGG AGCCCCAACC GCAGAGAGAG 
GGGGCCCAGA CCACCACAGG AAGGGAGAGA GAACCATATG GGAATAGGGC CACAGACTCT 
GGAGACAACC TCCACGATTC TGAAGATCTG GACCTACCAG CTACCCAGTG CTTTGTAGAC 
AGAAAGAATC AGAGCCTGGA AGCAGCCCCC AGCATGGAGG ATGAGCCCAC CCAGGCCTTC 
CTGTTTCCTC TGCCCCAAGA GCCTGGCCCT TCCTGTTGCA GCTCCCAGGC CACAGGTTCC 
TTGGATGAGT CATGGGAGGC GTTGGCGACA CAGCCATTCT GTCCGAGAGA ATCTGAGGCC 
TCCGAGACCC AAACCGTTGT CACCCTCCTT GACACCCATG CATCTTGGCC CTCTCCATCT 
AGGACAGCAC AGCAAGAGCA ACATCCAGAG AGCCCAGTCC ATGCAGAGCC ACTGGGGATG 
GAAGGCAGAG AGATGCAGAC TGTGGAGAAA GAAATGGGTA CCCCAAGAGA AACAGCAGAG 
AGGGTGATCC CTGAGGGAGG GCCACCGCAG GAGGAAACCA AGAAACTGCC CTCAGAAGGA 
GAGAGGGAAG ATGTGACGGG AGAGGAAGAA TTAATCGGGG GGATACAGGG CAGAGAAAAA 
AATCAGGTGT TTCCTAGAGA TACTCAGAGC CAAGAATCTG ACAAAAAAGT GAAAAGTGCA 
AGTACTGGAA GGGGAATGGA GATTGTAAAG GTAGAAATTG AGACACCCAA GGAAACACAA 
GAGAAAGAGA GAGAAAAGCA GACTCTCGCA GGGGAAATAT TTGAGAGTGA AGCAGGGAAA 
CTGGTAATAG AGAGAGAGAG CGAGGCAGAT GGGTTAGAAG TCAAGGAACC CCAAGAGCTA 
CTGGACAGAA GCCCACAGAT CGGGGAGACA GAGGCGGGGG ACCAGGACCA GAAAGGCCAA 
GCCTCTGGTC TGCCATCAGA GCCTGGAGCA GGGGCAGGAG ACCTTCAGGG ACTTGCTTCA 
GACCCAGTAG CTTTTGGGAG CCAGGCAGGT GGAGGAAGGG GAGCCCCAGG GAGCCCCAGG 
AGGCAGCAGA GAGGTGACTT GAATTGCGAG ATGCCACCTG CTCAGAAGGC TTCCAGGGGT 
GATCAGGAAT CCCCAGATGC TTGTCTGCCT CCTGCAGCGC CTGAAGCCTC AGCCGCACTC 
CCAAATTCCC TCATCTCTCA GATCCAAAAA CATCCCGCAC CTCAGTCCCT CCTTTTTCCC 
TCTCTAGCTC CTTTAGAACT GCCTATTCCC AGGACCAGAC AAAATGAGAC TCAGGAAGCT 
CCAGAGACTC CCTTCTCCTC AGAGCTGAAC TCTGTCCACC CAGAACCCAA AGTCAGGCTC 
CAGGCATCCT CTCCAGTTTC TTCTCTACCC CTTGAGCCCC ACCCTACCAC CCCCACAGGC 
CAGCCTATTG CCCTTGAACC CATATCTGGG GTCTCTCGGA GTAGGACATG TAGTTCCTTT 
GATGTAACTG CCTCATCAGT TGTCCCCACA GCCGTTGCAC TGCAGCCATC CACCTCCACA 
GACAAGCCTG TCACCCCTAA GCCCACACTT CGGGCCCCTC GGGGCAGGGC ACAGAGGTCT 
TCTGTCAAAA CCCCTGTACC CAGTGTCACC ACAGACCAGC CTGTTGCCCC TGAGCTCACA 
GCTATGGCAA CTCGGGGCAG GGTACAGAGG TCTTCTGTCA AGATTCCCAA ACCAGATAAC 
CCCACAACAC CCAAGCCCCA GCCTTCCACT TCTACAGACC AGCCTGTCAT CCCCAAACCC 
ACATCTCGGG CCCCTCGGGG CAGGACACCT AGGTCTTCTG CCAAGACTCC TGAACCAGCT 
GTCCCCACAG CCTCTGAGCT CCAGCCTGCT GCCCCTAAAG ACCAGCCTGT TGCTCCTGAG 
CTCACATCTA GAGCCACTCG GGGCAGGACA CAGAGGTCTA TCAAGACTTC CAAACCAGAT 
ATGCCCACAA CTCCCGAGCC CCAGCCTTCC ACTTCCACAG ACCAGCCTGT CACCCCCAAA 
CCCACGTCTC GGGCCCCTCG GGGCAGGACA CCTAGGTCTT CCGCCAAGAC TCCTGAACCA 
GTTGTCTCCA CAGCCTCTGA GCTCCAGCCT TCTGCCCTCA CAGATGAGCC TGTCACTCCT 
GAGCTCACAT CTAGGGCTAC TCGGGGCCGG GCACAGAGGT CCTCTGTCAA AACCCCTGAT 
CCAGTTACCA CCACAACACC TGAGCTCCAG CCTTCCACCT CCACAGACCA GCTTGTCACC 
CCCAAACGCA CATCTCGGGC CCCTCGAGGC AGGACACGTA GGTTGTCTGC CAAGACTCCT 
GAACCAGTTG TTCCCACAGC CTCTGAGCTC CAGCCTTCTG CCCCTGCAGA CCAGCCTGTT 
GGTCCTTGGG CCACTCAGTG TAGAAGACAT AGGTCTTCTG TCAAGACCCC AGAACCAGTT 
GTCCCCACAG TCCCTGAACC TCAGCCTTCC ACTTCTAAAG ACCAGTCTGT CACTCCTGAG 
CCCACATCTC AGGCCACTGA GAGCCAAACA CATAGGTCCT CTATCAAGAC ATCCCAGCCA 
ACGGAACCCA CAGCCCCTGA CCTCAAACCT TCCTCCCCCA CAGACCAGCT TGTCACTCCC 
AAGGTCATAG CTCAGGGTGG TCCAAGCAGG ACAGGAAGGG CTTCCACAGC AAGTGCTGTG 
CTGGTTCCTA CTACCCCTGA ATTCCAGTCT CCAGTCCCGT CAGAACAGCC TCTTCCCCCT 
GACCCCATCC CCGAAGTCAA CTGCAGCAGG AGGCTGAGGG CCACTAGGAA ACATGGGTCT 
CCCACAGCTC ATGTTCATGA GCCCTGCACC GTACCCCCTG AACCTAACTC TCACTCTTCA 
AGGAACCAAA GACATGGGGC AGTGAAAGCA GCCAAGCCCC TTAGCACCAT TCCTGAGCCT 
GCCTTTGCCC AGCTCCCCGA GGCACCGCCT CACACTCCCC AGGTGCCAAA GGAGGAGGCA 
GCAGATGGAT CAGGCTTCAC CCCAGAGCCC CAGCCTAGGG CCTCTCAAAA CCACAAGAGG 
CCTTCAGCTA CTGCACATTC ACCTCCACTT CAAAAACGGC TCCAGAGAGG GAGAGTCCCT 
CAGAAGGCAG CATCCCTTAA GGAAGAAGAA GAAAATCCAG TAGCGAGGCC GAGGAAGGAA 
GAGGGTGTAG TGATTCCAGG TCCAGGCAAG AGAAAGAGAG AGCAGACAGA AGAGGAGTCC 
CAGGGAAGAC CGAGCCGCAG CCTGCGACGG ACCAAACCGA TGCAGGAGTC CACGGCCCCC 
AAAGTGCTGT TCACCGGTGT GGTGGATGCT CGCGGAGAGA GGACGGTGCT GGCCTTGGGG 
GGCAGTTTGG CCAGCTCGGT GGCCGAGGCT TCTCACCTGG TGACTGATCG GATCCGCCGG 
ACGGTCAAGT TTCTGTGTGC CCTGGGCCGG GGCATCCCCA TCCTCTCCCT GGCCTGGCTG 
CATGAGTCCC GCAAGGCAGG CTGCTTCTTG CCCCCGGACG AATATTTGGT GACTGATCCT 
GAGCAGGAGA AGAACTTCGG CTTCAGCCTT CGGGAGGCCC TGAGCCGAGC TCAGGAGCGA 
AGGCTGCTGG AGGGCTATGA GATTCACGTG ACCCCCGGAG TCCAGCCACC GCCACCTCAG 
ATGGGAGAAA TCATCAGCTG CTGTGGAGGC GCCATCCTGC CCAGCATGCC CCGGTCCTAC 
AAGCCTCAGA GGTTCGTGAT CACATGTTCC CAGGACTTTC CTCGATGTGC TGTTCCATAT 
CGGGTTGGGC TGCCTATCCT CTCACCCGAA TTCCTGCTGA CGGGAGTACT GAAGCAGGAA 
GTCAAGCCAG AGGCCTTTGC CTTCTCCACT GTGGAAATGT CATCCACCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_027814660.1
CDS	274..6084
Translation	MEVSHFPCLHFPFKIMEDTQILNWEVEEEEEVAESPNESLGYSLEPLAQLHIFSSSYGPEKDFPLYLGKNMIGRMPDCSVALPFSSISKQHAVIEISAWDKAPVLRDCGSLNGTQILRPPKVLSPGVSHRLRDRELILFADLPCQYHRLDVPRPFVSRGPLSVEETPRVQGGTQPSRLLLAEDSEEEVDSLLDKCVVKGPRTSSSATVVPESDEEGPSPAPDGPGPPSAFNLNSDTDEEESQESGAGEASSAARRGTAAETEQPEPVTAEIQIEKDQCSVKERNGDTEIERDVRNGVVPTGVILERSQPSGEDSDTDVSDESGPRRRLVGVRPKRAWSCNFIDSDTDGEDEGIPATPAVVPMKERQIFHEAGTQSPQAPGVARWQGSPADGDTDIEEGEAPPDRSQASVVIDSNTDDEEEVSAALTLACLRESQAGKWNRDPDAEETRAQPVALLERSQASAGGDSDTDVEEEGLPVERRGMVPKGYMDREYSKKSQHLPRDSDTEGKEDESSPGVHLERSQASAQVEDEVPLGPAVALPEKRQVQGIVWPHHTDAEAEGDPAQLPVLRLEEARPPLAGDCELDSENTSAVRKSQLPAEKDAGTTWAAAVPEQDRALAIGTQGGSSTAPGEQDLLPVSREDLADMVVDIGTPGEPQPQREGAQTTTGREREPYGNRATDSGDNLHDSEDLDLPATQCFVDRKNQSLEAAPSMEDEPTQAFLFPLPQEPGPSCCSSQATGSLDESWEALATQPFCPRESEASETQTVVTLLDTHASWPSPSRTAQQEQHPESPVHAEPLGMEGREMQTVEKEMGTPRETAERVIPEGGPPQEETKKLPSEGEREDVTGEEELIGGIQGREKNQVFPRDTQSQESDKKVKSASTGRGMEIVKVEIETPKETQEKEREKQTLAGEIFESEAGKLVIERESEADGLEVKEPQELLDRSPQIGETEAGDQDQKGQASGLPSEPGAGAGDLQGLASDPVAFGSQAGGGRGAPGSPRRQQRGDLNCEMPPAQKASRGDQESPDACLPPAAPEASAALPNSLISQIQKHPAPQSLLFPSLAPLELPIPRTRQNETQEAPETPFSSELNSVHPEPKVRLQASSPVSSLPLEPHPTTPTGQPIALEPISGVSRSRTCSSFDVTASSVVPTAVALQPSTSTDKPVTPKPTLRAPRGRAQRSSVKTPVPSVTTDQPVAPELTAMATRGRVQRSSVKIPKPDNPTTPKPQPSTSTDQPVIPKPTSRAPRGRTPRSSAKTPEPAVPTASELQPAAPKDQPVAPELTSRATRGRTQRSIKTSKPDMPTTPEPQPSTSTDQPVTPKPTSRAPRGRTPRSSAKTPEPVVSTASELQPSALTDEPVTPELTSRATRGRAQRSSVKTPDPVTTTTPELQPSTSTDQLVTPKRTSRAPRGRTRRLSAKTPEPVVPTASELQPSAPADQPVGPWATQCRRHRSSVKTPEPVVPTVPEPQPSTSKDQSVTPEPTSQATESQTHRSSIKTSQPTEPTAPDLKPSSPTDQLVTPKVIAQGGPSRTGRASTASAVLVPTTPEFQSPVPSEQPLPPDPIPEVNCSRRLRATRKHGSPTAHVHEPCTVPPEPNSHSSRNQRHGAVKAAKPLSTIPEPAFAQLPEAPPHTPQVPKEEAADGSGFTPEPQPRASQNHKRPSATAHSPPLQKRLQRGRVPQKAASLKEEEENPVARPRKEEGVVIPGPGKRKREQTEEESQGRPSRSLRRTKPMQESTAPKVLFTGVVDARGERTVLALGGSLASSVAEASHLVTDRIRRTVKFLCALGRGIPILSLAWLHESRKAGCFLPPDEYLVTDPEQEKNFGFSLREALSRAQERRLLEGYEIHVTPGVQPPPPQMGEIISCCGGAILPSMPRSYKPQRFVITCSQDFPRCAVPYRVGLPILSPEFLLTGVLKQEVKPEAFAFSTVEMSST

Target ORF information:

RefSeq Version	XM_027958859.1
Organism	Ovis aries(sheep)
Definition	Ovis aries mediator of DNA damage checkpoint 1 (MDC1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027958859.1

ORF Insert Sequence:

ATGGAGGTGT CCCACTTTCC TTGTCTACAT TTTCCCTTTA AGATCATGGA AGACACCCAG 
ATTTTAAACT GGGAAGTTGA AGAAGAGGAG GAGGTTGCAG AGAGCCCCAA TGAATCTCTG 
GGGTATAGCT TGGAGCCCCT AGCACAGCTG CATATCTTCA GTAGTTCCTA TGGACCAGAA 
AAAGACTTCC CACTCTACCT TGGAAAGAAC ATGATAGGCC GAATGCCTGA TTGCTCTGTG 
GCCCTGCCCT TTTCATCAAT ATCCAAACAA CATGCAGTGA TTGAAATCTC TGCCTGGGAC 
AAGGCGCCTG TCCTTCGGGA TTGCGGGAGC CTCAATGGCA CTCAAATCCT GCGGCCTCCT 
AAGGTCCTGA GCCCTGGGGT GAGTCATCGT TTGCGGGACC GGGAGTTGAT TCTCTTTGCT 
GACTTGCCCT GCCAGTACCA TCGCTTGGAT GTCCCCCGGC CTTTTGTCTC CCGGGGCCCT 
CTAAGTGTAG AGGAGACACC CAGGGTACAG GGAGGAACTC AACCCTCCAG GCTTCTGTTG 
GCTGAGGACT CAGAGGAAGA AGTAGATTCC CTTTTGGACA AGTGTGTGGT GAAAGGACCA 
AGGACCTCCT CTTCGGCAAC AGTCGTTCCA GAGAGTGATG AAGAAGGGCC TTCCCCTGCC 
CCAGATGGGC CCGGGCCACC TTCTGCCTTC AACTTGAACA GCGACACGGA TGAGGAAGAA 
AGTCAGGAAT CAGGAGCAGG GGAGGCCTCT TCAGCTGCCA GAAGAGGTAC CGCTGCAGAG 
ACGGAACAGC CTGAACCTGT CACAGCCGAG ATCCAGATTG AGAAGGATCA GTGTTCCGTG 
AAGGAGAGGA ACGGGGACAC AGAAATCGAG AGGGATGTGA GGAATGGGGT GGTTCCGACT 
GGAGTGATTC TGGAGAGGAG CCAGCCTTCT GGGGAGGACA GTGACACAGA TGTGAGTGAT 
GAGAGTGGGC CTCGAAGAAG GCTTGTTGGG GTCCGTCCGA AAAGGGCCTG GTCTTGTAAC 
TTCATAGACA GTGATACCGA TGGAGAGGAC GAGGGGATCC CTGCTACCCC AGCAGTGGTT 
CCCATGAAGG AGAGGCAGAT CTTCCACGAA GCTGGTACAC AGAGCCCCCA GGCACCTGGT 
GTGGCACGTT GGCAGGGGAG CCCAGCTGAT GGTGATACAG ATATAGAGGA GGGGGAGGCC 
CCCCCAGACA GAAGCCAAGC CTCCGTGGTG ATCGACAGCA ATACAGACGA TGAGGAAGAA 
GTCTCAGCAG CACTGACACT GGCATGTCTA AGAGAGAGCC AGGCTGGTAA GTGGAACCGA 
GATCCAGATG CAGAAGAGAC CAGGGCCCAA CCCGTGGCCC TTCTGGAGCG AAGCCAGGCC 
TCTGCTGGGG GAGACAGTGA CACAGATGTG GAGGAAGAGG GGCTCCCAGT GGAAAGGAGG 
GGAATGGTTC CCAAGGGTTA CATGGACAGG GAATATTCAA AAAAGAGCCA GCACCTTCCC 
AGGGACAGTG ATACAGAGGG GAAGGAAGAT GAGAGCTCAC CGGGGGTCCA CCTGGAGAGA 
AGCCAGGCCT CTGCACAAGT GGAGGATGAG GTCCCACTGG GGCCAGCTGT TGCACTTCCG 
GAGAAGCGTC AGGTACAAGG CATAGTGTGG CCACATCACA CCGATGCGGA GGCAGAAGGG 
GACCCGGCAC AGCTCCCCGT GCTGCGTCTA GAGGAAGCCC GGCCTCCTCT AGCTGGGGAC 
TGTGAACTGG ATTCGGAGAA CACATCTGCT GTCAGAAAGA GCCAGCTTCC GGCGGAAAAA 
GATGCTGGGA CCACGTGGGC TGCAGCCGTT CCTGAACAGG ACAGAGCACT TGCCATCGGG 
ACCCAGGGTG GGTCATCCAC GGCACCAGGG GAGCAAGACC TTCTCCCGGT CTCAAGGGAA 
GACCTAGCAG ATATGGTGGT GGACATAGGC ACTCCAGGGG AGCCCCAACC GCAGAGAGAG 
GGGGCCCAGA CCACCACAGG AAGGGAGAGA GAACCATATG GGAATAGGGC CACAGACTCT 
GGAGACAACC TCCACGATTC TGAAGATCTG GACCTACCAG CTACCCAGTG CTTTGTAGAC 
AGAAAGAATC AGAGCCTGGA AGCAGCCCCC AGCATGGAGG ATGAGCCCAC CCAGGCCTTC 
CTGTTTCCTC TGCCCCAAGA GCCTGGCCCT TCCTGTTGCA GCTCCCAGGC CACAGGTTCC 
TTGGATGAGT CATGGGAGGC GTTGGCGACA CAGCCATTCT GTCCGAGAGA ATCTGAGGCC 
TCCGAGACCC AAACCGTTGT CACCCTCCTT GACACCCATG CATCTTGGCC CTCTCCATCT 
AGGACAGCAC AGCAAGAGCA ACATCCAGAG AGCCCAGTCC ATGCAGAGCC ACTGGGGATG 
GAAGGCAGAG AGATGCAGAC TGTGGAGAAA GAAATGGGTA CCCCAAGAGA AACAGCAGAG 
AGGGTGATCC CTGAGGGAGG GCCACCGCAG GAGGAAACCA AGAAACTGCC CTCAGAAGGA 
GAGAGGGAAG ATGTGACGGG AGAGGAAGAA TTAATCGGGG GGATACAGGG CAGAGAAAAA 
AATCAGGTGT TTCCTAGAGA TACTCAGAGC CAAGAATCTG ACAAAAAAGT GAAAAGTGCA 
AGTACTGGAA GGGGAATGGA GATTGTAAAG GTAGAAATTG AGACACCCAA GGAAACACAA 
GAGAAAGAGA GAGAAAAGCA GACTCTCGCA GGGGAAATAT TTGAGAGTGA AGCAGGGAAA 
CTGGTAATAG AGAGAGAGAG CGAGGCAGAT GGGTTAGAAG TCAAGGAACC CCAAGAGCTA 
CTGGACAGAA GCCCACAGAT CGGGGAGACA GAGGCGGGGG ACCAGGACCA GAAAGGCCAA 
GCCTCTGGTC TGCCATCAGA GCCTGGAGCA GGGGCAGGAG ACCTTCAGGG ACTTGCTTCA 
GACCCAGTAG CTTTTGGGAG CCAGGCAGGT GGAGGAAGGG GAGCCCCAGG GAGCCCCAGG 
AGGCAGCAGA GAGGTGACTT GAATTGCGAG ATGCCACCTG CTCAGAAGGC TTCCAGGGGT 
GATCAGGAAT CCCCAGATGC TTGTCTGCCT CCTGCAGCGC CTGAAGCCTC AGCCGCACTC 
CCAAATTCCC TCATCTCTCA GATCCAAAAA CATCCCGCAC CTCAGTCCCT CCTTTTTCCC 
TCTCTAGCTC CTTTAGAACT GCCTATTCCC AGGACCAGAC AAAATGAGAC TCAGGAAGCT 
CCAGAGACTC CCTTCTCCTC AGAGCTGAAC TCTGTCCACC CAGAACCCAA AGTCAGGCTC 
CAGGCATCCT CTCCAGTTTC TTCTCTACCC CTTGAGCCCC ACCCTACCAC CCCCACAGGC 
CAGCCTATTG CCCTTGAACC CATATCTGGG GTCTCTCGGA GTAGGACATG TAGTTCCTTT 
GATGTAACTG CCTCATCAGT TGTCCCCACA GCCGTTGCAC TGCAGCCATC CACCTCCACA 
GACAAGCCTG TCACCCCTAA GCCCACACTT CGGGCCCCTC GGGGCAGGGC ACAGAGGTCT 
TCTGTCAAAA CCCCTGTACC CAGTGTCACC ACAGACCAGC CTGTTGCCCC TGAGCTCACA 
GCTATGGCAA CTCGGGGCAG GGTACAGAGG TCTTCTGTCA AGATTCCCAA ACCAGATAAC 
CCCACAACAC CCAAGCCCCA GCCTTCCACT TCTACAGACC AGCCTGTCAT CCCCAAACCC 
ACATCTCGGG CCCCTCGGGG CAGGACACCT AGGTCTTCTG CCAAGACTCC TGAACCAGCT 
GTCCCCACAG CCTCTGAGCT CCAGCCTGCT GCCCCTAAAG ACCAGCCTGT TGCTCCTGAG 
CTCACATCTA GAGCCACTCG GGGCAGGACA CAGAGGTCTA TCAAGACTTC CAAACCAGAT 
ATGCCCACAA CTCCCGAGCC CCAGCCTTCC ACTTCCACAG ACCAGCCTGT CACCCCCAAA 
CCCACGTCTC GGGCCCCTCG GGGCAGGACA CCTAGGTCTT CCGCCAAGAC TCCTGAACCA 
GTTGTCTCCA CAGCCTCTGA GCTCCAGCCT TCTGCCCTCA CAGATGAGCC TGTCACTCCT 
GAGCTCACAT CTAGGGCTAC TCGGGGCCGG GCACAGAGGT CCTCTGTCAA AACCCCTGAT 
CCAGTTACCA CCACAACACC TGAGCTCCAG CCTTCCACCT CCACAGACCA GCTTGTCACC 
CCCAAACGCA CATCTCGGGC CCCTCGAGGC AGGACACGTA GGTTGTCTGC CAAGACTCCT 
GAACCAGTTG TTCCCACAGC CTCTGAGCTC CAGCCTTCTG CCCCTGCAGA CCAGCCTGTT 
GGTCCTTGGG CCACTCAGTG TAGAAGACAT AGGTCTTCTG TCAAGACCCC AGAACCAGTT 
GTCCCCACAG TCCCTGAACC TCAGCCTTCC ACTTCTAAAG ACCAGTCTGT CACTCCTGAG 
CCCACATCTC AGGCCACTGA GAGCCAAACA CATAGGTCCT CTATCAAGAC ATCCCAGCCA 
ACGGAACCCA CAGCCCCTGA CCTCAAACCT TCCTCCCCCA CAGACCAGCT TGTCACTCCC 
AAGGTCATAG CTCAGGGTGG TCCAAGCAGG ACAGGAAGGG CTTCCACAGC AAGTGCTGTG 
CTGGTTCCTA CTACCCCTGA ATTCCAGTCT CCAGTCCCGT CAGAACAGCC TCTTCCCCCT 
GACCCCATCC CCGAAGTCAA CTGCAGCAGG AGGCTGAGGG CCACTAGGAA ACATGGGTCT 
CCCACAGCTC ATGTTCATGA GCCCTGCACC GTACCCCCTG AACCTAACTC TCACTCTTCA 
AGGAACCAAA GACATGGGGC AGTGAAAGCA GCCAAGCCCC TTAGCACCAT TCCTGAGCCT 
GCCTTTGCCC AGCTCCCCGA GGCACCGCCT CACACTCCCC AGGTGCCAAA GGAGGAGGCA 
GCAGATGGAT CAGGCTTCAC CCCAGAGCCC CAGCCTAGGG CCTCTCAAAA CCACAAGAGG 
CCTTCAGCTA CTGCACATTC ACCTCCACTT CAAAAACGGC TCCAGAGAGG GAGAGTCCCT 
CAGAAGGCAG CATCCCTTAA GGAAGAAGAA GAAAATCCAG TAGCGAGGCC GAGGAAGGAA 
GAGGGTGTAG TGATTCCAGG TCCAGGCAAG AGAAAGAGAG AGCAGACAGA AGAGGAGTCC 
CAGGGAAGAC CGAGCCGCAG CCTGCGACGG ACCAAACCGA TGCAGGAGTC CACGGCCCCC 
AAAGTGCTGT TCACCGGTGT GGTGGATGCT CGCGGAGAGA GGACGGTGCT GGCCTTGGGG 
GGCAGTTTGG CCAGCTCGGT GGCCGAGGCT TCTCACCTGG TGACTGATCG GATCCGCCGG 
ACGGTCAAGT TTCTGTGTGC CCTGGGCCGG GGCATCCCCA TCCTCTCCCT GGCCTGGCTG 
CATGAGTCCC GCAAGGCAGG CTGCTTCTTG CCCCCGGACG AATATTTGGT GACTGATCCT 
GAGCAGGAGA AGAACTTCGG CTTCAGCCTT CGGGAGGCCC TGAGCCGAGC TCAGGAGCGA 
AGGCTGCTGG AGGGCTATGA GATTCACGTG ACCCCCGGAG TCCAGCCACC GCCACCTCAG 
ATGGGAGAAA TCATCAGCTG CTGTGGAGGC GCCATCCTGC CCAGCATGCC CCGGTCCTAC 
AAGCCTCAGA GGTTCGTGAT CACATGTTCC CAGGACTTTC CTCGATGTGC TGTTCCATAT 
CGGGTTGGGC TGCCTATCCT CTCACCCGAA TTCCTGCTGA CGGGAGTACT GAAGCAGGAA 
GTCAAGCCAG AGGCCTTTGC CTTCTCCACT GTGGAAATGT CATCCACCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MDC1 ( XM_015090177.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_014945663.1 Ovis aries musimon mediator of DNA damage checkpoint 1 (MDC1), transcript variant X11, mRNA.

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CloneID	OOv35148
Clone ID Related Accession (Same CDS sequence)	XM_015090177.1 , XM_012164401.2 , XM_012164402.2
Accession Version	XM_015090177.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5811bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	mediator of DNA damage checkpoint protein 1 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011943095.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGTGT CCCACTTTCC TTGTCTACAT TTTCCCTTTA AGATCATGGA AGACACCCAG 
ATTTTAAACT GGGAAGTTGA AGAAGAGGAG GAGGTTGCAG AGAGCCCCAA TGAATCTCTG 
GGGTATAGCT TGGAGCCCCT AGCACAGCTG CATATCTTCA GTAGTTCCTA TGGACCAGAA 
AAAGACTTCC CACTCTACCT TGGAAAGAAC ATGATAGGCC GAATGCCTGA TTGCTCTGTG 
GCCCTGCCCT TTTCATCAAT ATCCAAACAA CATGCAGTGA TTGAAATCTC TGCCTGGGAC 
AAGGCGCCTG TCCTTCGGGA TTGCGGGAGC CTCAATGGCA CTCAAATCCT GCGGCCTCCT 
AAGGTCCTGA GCCCTGGGGT GAGTCATCGT TTGCGGGACC GGGAGTTGAT TCTCTTTGCT 
GACTTGCCCT GCCAGTACCA TCGCTTGGAT GTCCCCCGGC CTTTTGTCTC CCGGGGCCCT 
CTAACTGTAG AGGAGACACC CAGGGTACAG GGAGGAACTC AACCCTCCAG GCTTCTGTTG 
GCTGAGGACT CAGAGGAAGA AGTAGATTCC CTTTTGGACA AGTGTGTGGT GAAAGGACCA 
AGGACCTCCT CTTCGGCAAC AGTCGTTCCA GAGAGTGATG AAGAAGGGCC TTCCCCTGCC 
CCAGATGGGC CCGGGCCACC TTCTGCCTTC AACTTGAACA GCGACACGGA TGAGGAAGAA 
AGTCAGGAAT CAGGAGCAGG GGAGGCCTCT TCAGCTGCCA GAAGAGGTAC CGCTGCAGAG 
ACGGAACAGC CTGAACCTGT CACAGCCGAG ATCCAGATTG AGAAGGATCA GTGTTCCGTG 
AAGGAGAGGA ACGGGGACAC AGAAATCGAG AGGGATGTGA GGAATGGGGT GGTTCCGACT 
GGAGTGATTC TGGAGAGGAG CCAGCCTTCT GGGGAGGACA GTGACACAGA TGTGAGTGAT 
GAGAGTGGGC CTCGAAGAAG GCTTGTTGGA GTCCGTCCGA AAAGGGCCTG GTCTTGTAAC 
TTCATAGACA GTGATACCGA TGGAGAGGAC GAGGGGATCC CTGCTACCCC AGCAGTGGTT 
CCCATGAAGG AGAGGCAGAT CTTCCACGAA GCTGGTACAC AGAGCCCCCA GGCACCTGGT 
GTGGCACGTT GGCAGGAGAG CCCAGCTGAT GGTGATACAG ATATAGAGGA GGGGGAGGCC 
CCCCCAGACA GAAGCCAAGC CTCCGTGGTG ATCGACAGCA ATACAGACGA TGAGGAAGAA 
GTCTCAGCAG CACTGACACT GGCATGTCTA AGAGAGAGCC AGGCTGGTAA GTGGAACCGA 
GATCCAGATG CAGAAGAGAC CAGGGCCCAA CCCGTGGCCC TTCTGGAGCG AAGCCAGGCC 
TCTGCTGGGG GAGACAGTGA CACAGATGTG GAGGAAGAGG GGCTCCCAGT GGAAAGGAGG 
GGAATGGTTC CCAAGGGTTA CATGGACAGG GAATATTCAA AAAAGAGCCA GCACCCTCCC 
AGGGACAGTG ATACAGAGGG GAAGGAAGAT GAGAGCTCAC CGGGGGTCCA CCTGGAGAGA 
AGCCAGGCCT CTGCACAAGT GGAGGATGAG GTCCCACTGG GGCCAGCTGT TGCACTTCCG 
GAGAAGCGTC AGGTACAAGG CATAGTGTGG CCACATCACA CCGATGCGGA GGCAGAAGGG 
GACCCGGCAC AGCTCCCCGT GCTGCGTCTA GAGGAAGCCC GGCCTCCTCT AGCTGGGGAC 
TGTGAACTGG ATTCGGAGAA CACATCTGCT GTCAGAAAGA GCCAGCTTCC GGCGGAAAAA 
GATGCTGGGA CCACGTGGGC TGCAGCCGTT CCTGAACAGG ACAGAGCACT TGCCATCGGG 
ACCCAGGGTG GGTCATCCAC GGCACCAGGG GAGCAAGACC TTCTCCCGGT CTCAAGGGAA 
GACCTAGCAG ATATGGTGGT GGACATAGGC ACTCCAGGGG AGCCCCAACC GCAGAGAGAG 
GGGGCCCAGA CCACCACAGG AAGGGAGAGA GAACCATATG GGAATAGGGC CACAGACTCT 
GGAGACAACC TCCACGATTC TGAAGATCTG GACCTACCAG CTACCCAGTG CTTTGTAGAC 
AGAAAGAATC AGAGCCTGGA AGCAGCCCCC AGCATGGAGG ATGAGCCCAC CCAGGCCTTC 
CTGTTTCCTC TGCCCCAAGA GCCTGGCCCT TCCTGTTGCA GCTCCCAGGC CACAGGTTCC 
TTGGATGAGT CATGGGAGGC CTTGGCGACA CAGCCATTCT GTCCGAGAGA ATCTGAGGCC 
TCCGAGACCC AAACCGTTGT CACCCTCCTT GACACCCATG CATCTTGGCC CTCTCCATCT 
AGGACAGCAC AGCAAGAGCA ACATCCAGAG AGCCCAGTCC ATGCAGAGCC ACTGGGGATG 
GAAGGCAGAG AGATGCAGAC TGTGGAGAAA GAAATGGGTA CCCCAAGAGA AACAGCAGAG 
AGGGTGATCC CTGAGGGAGG GCCACCGCAG GAGGAAACCA AGAAACTGCC CTCAGAAGGA 
GAGAGGGAAG ATGTGACGGG AGAGGAAGAA TTAATCGGGG GGATACAGGG CAGAGAAAAA 
AATCAGGTGT TTGCTAGAGA TACTCAGAGC CAAGAATCTG ACAAAAAAGT GAAAAGTGCA 
AGTACTGGAA GGGGAATGGA GATTTTAAAG GTAGAAATTG AGACACCCAA GGAAACACAA 
GAGAAAGAGA GAGAAAAGCA GACTCTCGCA GGGGAAATAT TTGAGAGTGA AGCAGGGAAA 
CTGGTAGTAG AGAGAGAGAG TGAGGCAGAT GGGTTAGAAG TCAAGGAACC CCAAGAGCTA 
CTGGACAGAA GCCCACAGAT GGGGGAGACA GAGGCGGGGG ACCAGGACCA GAAAGGCCAA 
GCCTCTGGTC TGCCATCAGA GCCTGGAGCA GGGGCAGGAG ACCTTCAGGG ACTTGCTTCA 
GACCCAGTAG CTTCTGGGAG CCAGGCAGGT GGAGGAAGGG GAGTCCCAGG GAGCCCCAGG 
AGGCAGCAGA GAGGTGACTT GAATTGCGAG ATGCCACCTG CTCAGAAGGC TTCCAGGGGT 
GATCAGGAAT CCCCAGATGC TTGTCTGCCT CCTGCAGCGC CTGAAGCCTC AGCCGCACTC 
CCAAATTCCC TCATCATCTC TCAGATCCAA AAACATCCCG CACCTCAGTC CCTCCTTTTT 
CCCTCTCTAG CTCCTTTAGA ACTGCCTATT CCCAGGACCA GACAAAATGA GACTCAGGAA 
GCTCCAGAGA CTCCCTTCTC CTCAGAGCTG AACTCTGTCC ACCCAGAACC CAAAGTCAGG 
CTCCAGGCGT CCTCTCCAGT TTCTTCTCTA CCCCTTGAGC CCCACCCTAC CACCCCCACA 
GGCCAGCCTA TTGCCCTTGA ACCCATATCT GGGGTCTCTT GGAGCAGGAC ACGTAGTTCC 
TTTGATGTAA CTGCCTCATC AGTTGTCCCC ACAGCCGTTG CACTGCAGCC ATCCATCTCC 
ACAGACAAGC CTGTCACCCC TAAGCCCACA CTTCGGGCCC CTCGGGGCAG GGCACAGAGG 
TCTTCTGTCA AAACCCCTGA ACCCAATGTC ACCACAGACC AGCCTGTTGC CCCTGAGCTC 
ACAGCTATGG CAACTCGGGG CAGGGCACAG AGGTCTTCTG TCAAGATTCC CAAACCAGAT 
AACCCCACAA CACCCAAGCC CCAGCCTTCC ACTTCCACAG ACCAGCCTGT CATCCCCAAA 
CCCACATCTC GGGCCCCTCG GGGCAGGACA CCTAGGTCTT CTGCCAAGAC TCCTGAACCA 
GCTGTCCCCA CAGCCTCTGA GCTCCAGCCT GCTGCCCCTA AAGACCAGCC TGTTGCTCCT 
GAGCTCACAT CTAGAGCCAC TCGGGGCAGG ACACAGAGGT CTATCAAGAC TTCCAAACCA 
GATATGCCCA CAACTCCCGA GCCCCAGCCT TCCACCTCCG CAGACCAGCC TGTCACCCCC 
AAACCCACGT CTCGGGCCCC TCGGGGCAGG ACACCTAGGT CTTCCGCCAA GACTCCTGAG 
CCAGTTGTCT CCACAGCCTC TGAGCTCCAG CCTTCTGCCC TCACAGATGA GCCTGTCACT 
CCTGAGCTCA CATCTAGGGC TACTCGGGGT CGGGCACAGA GGTCCTCTGT CAAAACCCCT 
GATCCAGTTA CCACCACAAC ACCTGAGCTC CAGCCTTCCA CCTCCACAGA CCAGCTTGTC 
ACCCCCAAAC GCACATCTCG GGCCCCTCGA GGCAGGACAC GTAGGTTGTC TGCCAAGACT 
CCTGAACCAG TTGTTCCCAC AGCCTCTGAG CTCCAGCCTT CTGCCCCTGC AGACCAGCCT 
GTTGGTCCTT GGGCCACTCA GTGTAGAAGA CATAGGTCTT CTGTCAAGAC CCCAGAACCA 
GTTGTCCCCA CAGTCCCTGA ACCTCAGCCT TCCACTTCTA AAGACCAGTC TGTCACTCCT 
GAGCCCACAT CTCAGGCCAC TGAGAGCCAA ACACATAGGT CCTCTATCAA GACATCCCAG 
CCAACGGAAC CCACAGCCTC TGACCTCAAA CCTTCCTCCC CCACAGACCA GCTTGTCACT 
CCCAAGGTCA TAGCTCAGGG TGGTCCAAGC AGGACAGGAA GGGCTTCCAC AGCAAGTGCT 
GTGCTGGTTC CTACTACCCC TGAATTCCAG TCTCCAGTCC CGTCAGAACA GCCTCTTCCC 
CCTGACCCCA TCCCTGAAGT CAACTGCAGC AGGAGGCTGA GGGCCACTAG GAAACATGGG 
TCTCCCACAG CTCATGTTCA TGAGCCCTGC ACCGTACCCC CTGAACCTAA CTCCCACTCT 
TCAAGGAACC AAAGACATGG GGCAGTGAAA GCAGCCAAGC CCCTTAGCAC CATTCCTGAG 
CCTGCCTTTG CCCAGCTCCC CGAGGCACCG CCTCACACTC CCCAGGTGCC AAAGGAGGAG 
GCAGCAGATG GATCAGGCTT CACCCCAGAG CCCCAGCCTA GGGCCTCTCA AAACCACAAG 
AGGCCTTCAG CTACTGCACA TTCACCTCCA CTTCAAAAAC GGCTCCAGAG AGGGAGAGTC 
CCTCAGAAGG CAGCATCCCT TAAGGAAGAA GAAAATCCAG TAGCGAGGCC GAGGAAGGAA 
GAGGGTGTAG TGATTCCAGG TCCAGGCAAG AGAAAGAGAG AGCAGACAGA GGAGGAGTCC 
CAGGGAAGAC CGAGCCGCAG CCTGCGACGG ACCAGACCGA TGCAGGAGTC CACGGCCCCC 
AAAGTGCTGT TCACCGGTGT GGTGGATGCT CGCGGAGAGA GGACGGTGCT GGCCCTGGGG 
GGCAGTTTGG CCAGCTCGGT GGCCGAGGCT TCTCACCTGG TGACTGATCG GATCCGCCGG 
ACGGTCAAGT TTCTGTGTGC CCTGGGCCGG GGCATCCCCA TCCTCTCCCT GGCCTGGCTG 
CATGAGTCCC GCAAGGCAGG CTGCTTCTTG CCCCCGGACG AATATTTGGT GACTGATCCT 
GAGCAGGAGA AGAACTTCGG CTTCAGCCTT CGGGAGGCCC TGAGCCGAGC TCAGGAGCGA 
AGGCTGCTGG AGGGCTATGA GATTCATGTG ACCCCCGGAG TCCAGCCACC GCCACCTCAG 
ATGGGAGAAA TCATCAGCTG CTGTGGAGGC GCCATCCTGC CCAGCATGCC TCGGTCCTAC 
AAGCCTCAGA GGTTCGTGAT CACATGTTCC CAGGACTTTC CTCGATGTGC TGTTCCATAT 
CGGGTTGGGC TGCCTATCCT CTCACCCGAA TTCCTGCTGA CGGGAGTACT GAAGCAGGAA 
GTCAAGCCAG AGGCCTTTGC CTTCTCCACT GTGGAAATGT CATCCACCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014945663.1
CDS	295..6105
Translation	MEVSHFPCLHFPFKIMEDTQILNWEVEEEEEVAESPNESLGYSLEPLAQLHIFSSSYGPEKDFPLYLGKNMIGRMPDCSVALPFSSISKQHAVIEISAWDKAPVLRDCGSLNGTQILRPPKVLSPGVSHRLRDRELILFADLPCQYHRLDVPRPFVSRGPLTVEETPRVQGGTQPSRLLLAEDSEEEVDSLLDKCVVKGPRTSSSATVVPESDEEGPSPAPDGPGPPSAFNLNSDTDEEESQESGAGEASSAARRGTAAETEQPEPVTAEIQIEKDQCSVKERNGDTEIERDVRNGVVPTGVILERSQPSGEDSDTDVSDESGPRRRLVGVRPKRAWSCNFIDSDTDGEDEGIPATPAVVPMKERQIFHEAGTQSPQAPGVARWQESPADGDTDIEEGEAPPDRSQASVVIDSNTDDEEEVSAALTLACLRESQAGKWNRDPDAEETRAQPVALLERSQASAGGDSDTDVEEEGLPVERRGMVPKGYMDREYSKKSQHPPRDSDTEGKEDESSPGVHLERSQASAQVEDEVPLGPAVALPEKRQVQGIVWPHHTDAEAEGDPAQLPVLRLEEARPPLAGDCELDSENTSAVRKSQLPAEKDAGTTWAAAVPEQDRALAIGTQGGSSTAPGEQDLLPVSREDLADMVVDIGTPGEPQPQREGAQTTTGREREPYGNRATDSGDNLHDSEDLDLPATQCFVDRKNQSLEAAPSMEDEPTQAFLFPLPQEPGPSCCSSQATGSLDESWEALATQPFCPRESEASETQTVVTLLDTHASWPSPSRTAQQEQHPESPVHAEPLGMEGREMQTVEKEMGTPRETAERVIPEGGPPQEETKKLPSEGEREDVTGEEELIGGIQGREKNQVFARDTQSQESDKKVKSASTGRGMEILKVEIETPKETQEKEREKQTLAGEIFESEAGKLVVERESEADGLEVKEPQELLDRSPQMGETEAGDQDQKGQASGLPSEPGAGAGDLQGLASDPVASGSQAGGGRGVPGSPRRQQRGDLNCEMPPAQKASRGDQESPDACLPPAAPEASAALPNSLIISQIQKHPAPQSLLFPSLAPLELPIPRTRQNETQEAPETPFSSELNSVHPEPKVRLQASSPVSSLPLEPHPTTPTGQPIALEPISGVSWSRTRSSFDVTASSVVPTAVALQPSISTDKPVTPKPTLRAPRGRAQRSSVKTPEPNVTTDQPVAPELTAMATRGRAQRSSVKIPKPDNPTTPKPQPSTSTDQPVIPKPTSRAPRGRTPRSSAKTPEPAVPTASELQPAAPKDQPVAPELTSRATRGRTQRSIKTSKPDMPTTPEPQPSTSADQPVTPKPTSRAPRGRTPRSSAKTPEPVVSTASELQPSALTDEPVTPELTSRATRGRAQRSSVKTPDPVTTTTPELQPSTSTDQLVTPKRTSRAPRGRTRRLSAKTPEPVVPTASELQPSAPADQPVGPWATQCRRHRSSVKTPEPVVPTVPEPQPSTSKDQSVTPEPTSQATESQTHRSSIKTSQPTEPTASDLKPSSPTDQLVTPKVIAQGGPSRTGRASTASAVLVPTTPEFQSPVPSEQPLPPDPIPEVNCSRRLRATRKHGSPTAHVHEPCTVPPEPNSHSSRNQRHGAVKAAKPLSTIPEPAFAQLPEAPPHTPQVPKEEAADGSGFTPEPQPRASQNHKRPSATAHSPPLQKRLQRGRVPQKAASLKEEENPVARPRKEEGVVIPGPGKRKREQTEEESQGRPSRSLRRTRPMQESTAPKVLFTGVVDARGERTVLALGGSLASSVAEASHLVTDRIRRTVKFLCALGRGIPILSLAWLHESRKAGCFLPPDEYLVTDPEQEKNFGFSLREALSRAQERRLLEGYEIHVTPGVQPPPPQMGEIISCCGGAILPSMPRSYKPQRFVITCSQDFPRCAVPYRVGLPILSPEFLLTGVLKQEVKPEAFAFSTVEMSST

Target ORF information:

RefSeq Version	XM_015090177.1
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon mediator of DNA damage checkpoint 1 (MDC1), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015090177.1

ORF Insert Sequence:

ATGGAGGTGT CCCACTTTCC TTGTCTACAT TTTCCCTTTA AGATCATGGA AGACACCCAG 
ATTTTAAACT GGGAAGTTGA AGAAGAGGAG GAGGTTGCAG AGAGCCCCAA TGAATCTCTG 
GGGTATAGCT TGGAGCCCCT AGCACAGCTG CATATCTTCA GTAGTTCCTA TGGACCAGAA 
AAAGACTTCC CACTCTACCT TGGAAAGAAC ATGATAGGCC GAATGCCTGA TTGCTCTGTG 
GCCCTGCCCT TTTCATCAAT ATCCAAACAA CATGCAGTGA TTGAAATCTC TGCCTGGGAC 
AAGGCGCCTG TCCTTCGGGA TTGCGGGAGC CTCAATGGCA CTCAAATCCT GCGGCCTCCT 
AAGGTCCTGA GCCCTGGGGT GAGTCATCGT TTGCGGGACC GGGAGTTGAT TCTCTTTGCT 
GACTTGCCCT GCCAGTACCA TCGCTTGGAT GTCCCCCGGC CTTTTGTCTC CCGGGGCCCT 
CTAACTGTAG AGGAGACACC CAGGGTACAG GGAGGAACTC AACCCTCCAG GCTTCTGTTG 
GCTGAGGACT CAGAGGAAGA AGTAGATTCC CTTTTGGACA AGTGTGTGGT GAAAGGACCA 
AGGACCTCCT CTTCGGCAAC AGTCGTTCCA GAGAGTGATG AAGAAGGGCC TTCCCCTGCC 
CCAGATGGGC CCGGGCCACC TTCTGCCTTC AACTTGAACA GCGACACGGA TGAGGAAGAA 
AGTCAGGAAT CAGGAGCAGG GGAGGCCTCT TCAGCTGCCA GAAGAGGTAC CGCTGCAGAG 
ACGGAACAGC CTGAACCTGT CACAGCCGAG ATCCAGATTG AGAAGGATCA GTGTTCCGTG 
AAGGAGAGGA ACGGGGACAC AGAAATCGAG AGGGATGTGA GGAATGGGGT GGTTCCGACT 
GGAGTGATTC TGGAGAGGAG CCAGCCTTCT GGGGAGGACA GTGACACAGA TGTGAGTGAT 
GAGAGTGGGC CTCGAAGAAG GCTTGTTGGA GTCCGTCCGA AAAGGGCCTG GTCTTGTAAC 
TTCATAGACA GTGATACCGA TGGAGAGGAC GAGGGGATCC CTGCTACCCC AGCAGTGGTT 
CCCATGAAGG AGAGGCAGAT CTTCCACGAA GCTGGTACAC AGAGCCCCCA GGCACCTGGT 
GTGGCACGTT GGCAGGAGAG CCCAGCTGAT GGTGATACAG ATATAGAGGA GGGGGAGGCC 
CCCCCAGACA GAAGCCAAGC CTCCGTGGTG ATCGACAGCA ATACAGACGA TGAGGAAGAA 
GTCTCAGCAG CACTGACACT GGCATGTCTA AGAGAGAGCC AGGCTGGTAA GTGGAACCGA 
GATCCAGATG CAGAAGAGAC CAGGGCCCAA CCCGTGGCCC TTCTGGAGCG AAGCCAGGCC 
TCTGCTGGGG GAGACAGTGA CACAGATGTG GAGGAAGAGG GGCTCCCAGT GGAAAGGAGG 
GGAATGGTTC CCAAGGGTTA CATGGACAGG GAATATTCAA AAAAGAGCCA GCACCCTCCC 
AGGGACAGTG ATACAGAGGG GAAGGAAGAT GAGAGCTCAC CGGGGGTCCA CCTGGAGAGA 
AGCCAGGCCT CTGCACAAGT GGAGGATGAG GTCCCACTGG GGCCAGCTGT TGCACTTCCG 
GAGAAGCGTC AGGTACAAGG CATAGTGTGG CCACATCACA CCGATGCGGA GGCAGAAGGG 
GACCCGGCAC AGCTCCCCGT GCTGCGTCTA GAGGAAGCCC GGCCTCCTCT AGCTGGGGAC 
TGTGAACTGG ATTCGGAGAA CACATCTGCT GTCAGAAAGA GCCAGCTTCC GGCGGAAAAA 
GATGCTGGGA CCACGTGGGC TGCAGCCGTT CCTGAACAGG ACAGAGCACT TGCCATCGGG 
ACCCAGGGTG GGTCATCCAC GGCACCAGGG GAGCAAGACC TTCTCCCGGT CTCAAGGGAA 
GACCTAGCAG ATATGGTGGT GGACATAGGC ACTCCAGGGG AGCCCCAACC GCAGAGAGAG 
GGGGCCCAGA CCACCACAGG AAGGGAGAGA GAACCATATG GGAATAGGGC CACAGACTCT 
GGAGACAACC TCCACGATTC TGAAGATCTG GACCTACCAG CTACCCAGTG CTTTGTAGAC 
AGAAAGAATC AGAGCCTGGA AGCAGCCCCC AGCATGGAGG ATGAGCCCAC CCAGGCCTTC 
CTGTTTCCTC TGCCCCAAGA GCCTGGCCCT TCCTGTTGCA GCTCCCAGGC CACAGGTTCC 
TTGGATGAGT CATGGGAGGC CTTGGCGACA CAGCCATTCT GTCCGAGAGA ATCTGAGGCC 
TCCGAGACCC AAACCGTTGT CACCCTCCTT GACACCCATG CATCTTGGCC CTCTCCATCT 
AGGACAGCAC AGCAAGAGCA ACATCCAGAG AGCCCAGTCC ATGCAGAGCC ACTGGGGATG 
GAAGGCAGAG AGATGCAGAC TGTGGAGAAA GAAATGGGTA CCCCAAGAGA AACAGCAGAG 
AGGGTGATCC CTGAGGGAGG GCCACCGCAG GAGGAAACCA AGAAACTGCC CTCAGAAGGA 
GAGAGGGAAG ATGTGACGGG AGAGGAAGAA TTAATCGGGG GGATACAGGG CAGAGAAAAA 
AATCAGGTGT TTGCTAGAGA TACTCAGAGC CAAGAATCTG ACAAAAAAGT GAAAAGTGCA 
AGTACTGGAA GGGGAATGGA GATTTTAAAG GTAGAAATTG AGACACCCAA GGAAACACAA 
GAGAAAGAGA GAGAAAAGCA GACTCTCGCA GGGGAAATAT TTGAGAGTGA AGCAGGGAAA 
CTGGTAGTAG AGAGAGAGAG TGAGGCAGAT GGGTTAGAAG TCAAGGAACC CCAAGAGCTA 
CTGGACAGAA GCCCACAGAT GGGGGAGACA GAGGCGGGGG ACCAGGACCA GAAAGGCCAA 
GCCTCTGGTC TGCCATCAGA GCCTGGAGCA GGGGCAGGAG ACCTTCAGGG ACTTGCTTCA 
GACCCAGTAG CTTCTGGGAG CCAGGCAGGT GGAGGAAGGG GAGTCCCAGG GAGCCCCAGG 
AGGCAGCAGA GAGGTGACTT GAATTGCGAG ATGCCACCTG CTCAGAAGGC TTCCAGGGGT 
GATCAGGAAT CCCCAGATGC TTGTCTGCCT CCTGCAGCGC CTGAAGCCTC AGCCGCACTC 
CCAAATTCCC TCATCATCTC TCAGATCCAA AAACATCCCG CACCTCAGTC CCTCCTTTTT 
CCCTCTCTAG CTCCTTTAGA ACTGCCTATT CCCAGGACCA GACAAAATGA GACTCAGGAA 
GCTCCAGAGA CTCCCTTCTC CTCAGAGCTG AACTCTGTCC ACCCAGAACC CAAAGTCAGG 
CTCCAGGCGT CCTCTCCAGT TTCTTCTCTA CCCCTTGAGC CCCACCCTAC CACCCCCACA 
GGCCAGCCTA TTGCCCTTGA ACCCATATCT GGGGTCTCTT GGAGCAGGAC ACGTAGTTCC 
TTTGATGTAA CTGCCTCATC AGTTGTCCCC ACAGCCGTTG CACTGCAGCC ATCCATCTCC 
ACAGACAAGC CTGTCACCCC TAAGCCCACA CTTCGGGCCC CTCGGGGCAG GGCACAGAGG 
TCTTCTGTCA AAACCCCTGA ACCCAATGTC ACCACAGACC AGCCTGTTGC CCCTGAGCTC 
ACAGCTATGG CAACTCGGGG CAGGGCACAG AGGTCTTCTG TCAAGATTCC CAAACCAGAT 
AACCCCACAA CACCCAAGCC CCAGCCTTCC ACTTCCACAG ACCAGCCTGT CATCCCCAAA 
CCCACATCTC GGGCCCCTCG GGGCAGGACA CCTAGGTCTT CTGCCAAGAC TCCTGAACCA 
GCTGTCCCCA CAGCCTCTGA GCTCCAGCCT GCTGCCCCTA AAGACCAGCC TGTTGCTCCT 
GAGCTCACAT CTAGAGCCAC TCGGGGCAGG ACACAGAGGT CTATCAAGAC TTCCAAACCA 
GATATGCCCA CAACTCCCGA GCCCCAGCCT TCCACCTCCG CAGACCAGCC TGTCACCCCC 
AAACCCACGT CTCGGGCCCC TCGGGGCAGG ACACCTAGGT CTTCCGCCAA GACTCCTGAG 
CCAGTTGTCT CCACAGCCTC TGAGCTCCAG CCTTCTGCCC TCACAGATGA GCCTGTCACT 
CCTGAGCTCA CATCTAGGGC TACTCGGGGT CGGGCACAGA GGTCCTCTGT CAAAACCCCT 
GATCCAGTTA CCACCACAAC ACCTGAGCTC CAGCCTTCCA CCTCCACAGA CCAGCTTGTC 
ACCCCCAAAC GCACATCTCG GGCCCCTCGA GGCAGGACAC GTAGGTTGTC TGCCAAGACT 
CCTGAACCAG TTGTTCCCAC AGCCTCTGAG CTCCAGCCTT CTGCCCCTGC AGACCAGCCT 
GTTGGTCCTT GGGCCACTCA GTGTAGAAGA CATAGGTCTT CTGTCAAGAC CCCAGAACCA 
GTTGTCCCCA CAGTCCCTGA ACCTCAGCCT TCCACTTCTA AAGACCAGTC TGTCACTCCT 
GAGCCCACAT CTCAGGCCAC TGAGAGCCAA ACACATAGGT CCTCTATCAA GACATCCCAG 
CCAACGGAAC CCACAGCCTC TGACCTCAAA CCTTCCTCCC CCACAGACCA GCTTGTCACT 
CCCAAGGTCA TAGCTCAGGG TGGTCCAAGC AGGACAGGAA GGGCTTCCAC AGCAAGTGCT 
GTGCTGGTTC CTACTACCCC TGAATTCCAG TCTCCAGTCC CGTCAGAACA GCCTCTTCCC 
CCTGACCCCA TCCCTGAAGT CAACTGCAGC AGGAGGCTGA GGGCCACTAG GAAACATGGG 
TCTCCCACAG CTCATGTTCA TGAGCCCTGC ACCGTACCCC CTGAACCTAA CTCCCACTCT 
TCAAGGAACC AAAGACATGG GGCAGTGAAA GCAGCCAAGC CCCTTAGCAC CATTCCTGAG 
CCTGCCTTTG CCCAGCTCCC CGAGGCACCG CCTCACACTC CCCAGGTGCC AAAGGAGGAG 
GCAGCAGATG GATCAGGCTT CACCCCAGAG CCCCAGCCTA GGGCCTCTCA AAACCACAAG 
AGGCCTTCAG CTACTGCACA TTCACCTCCA CTTCAAAAAC GGCTCCAGAG AGGGAGAGTC 
CCTCAGAAGG CAGCATCCCT TAAGGAAGAA GAAAATCCAG TAGCGAGGCC GAGGAAGGAA 
GAGGGTGTAG TGATTCCAGG TCCAGGCAAG AGAAAGAGAG AGCAGACAGA GGAGGAGTCC 
CAGGGAAGAC CGAGCCGCAG CCTGCGACGG ACCAGACCGA TGCAGGAGTC CACGGCCCCC 
AAAGTGCTGT TCACCGGTGT GGTGGATGCT CGCGGAGAGA GGACGGTGCT GGCCCTGGGG 
GGCAGTTTGG CCAGCTCGGT GGCCGAGGCT TCTCACCTGG TGACTGATCG GATCCGCCGG 
ACGGTCAAGT TTCTGTGTGC CCTGGGCCGG GGCATCCCCA TCCTCTCCCT GGCCTGGCTG 
CATGAGTCCC GCAAGGCAGG CTGCTTCTTG CCCCCGGACG AATATTTGGT GACTGATCCT 
GAGCAGGAGA AGAACTTCGG CTTCAGCCTT CGGGAGGCCC TGAGCCGAGC TCAGGAGCGA 
AGGCTGCTGG AGGGCTATGA GATTCATGTG ACCCCCGGAG TCCAGCCACC GCCACCTCAG 
ATGGGAGAAA TCATCAGCTG CTGTGGAGGC GCCATCCTGC CCAGCATGCC TCGGTCCTAC 
AAGCCTCAGA GGTTCGTGAT CACATGTTCC CAGGACTTTC CTCGATGTGC TGTTCCATAT 
CGGGTTGGGC TGCCTATCCT CTCACCCGAA TTCCTGCTGA CGGGAGTACT GAAGCAGGAA 
GTCAAGCCAG AGGCCTTTGC CTTCTCCACT GTGGAAATGT CATCCACCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MDC1 ( XM_012164401.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_012019791.1 Ovis aries musimon mediator of DNA damage checkpoint 1 (MDC1), transcript variant X9, mRNA.

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CloneID	OOv35148
Clone ID Related Accession (Same CDS sequence)	XM_015090177.1 , XM_012164401.2 , XM_012164402.2
Accession Version	XM_012164401.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5811bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	mediator of DNA damage checkpoint protein 1 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011943095.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012164401.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGTGT CCCACTTTCC TTGTCTACAT TTTCCCTTTA AGATCATGGA AGACACCCAG 
ATTTTAAACT GGGAAGTTGA AGAAGAGGAG GAGGTTGCAG AGAGCCCCAA TGAATCTCTG 
GGGTATAGCT TGGAGCCCCT AGCACAGCTG CATATCTTCA GTAGTTCCTA TGGACCAGAA 
AAAGACTTCC CACTCTACCT TGGAAAGAAC ATGATAGGCC GAATGCCTGA TTGCTCTGTG 
GCCCTGCCCT TTTCATCAAT ATCCAAACAA CATGCAGTGA TTGAAATCTC TGCCTGGGAC 
AAGGCGCCTG TCCTTCGGGA TTGCGGGAGC CTCAATGGCA CTCAAATCCT GCGGCCTCCT 
AAGGTCCTGA GCCCTGGGGT GAGTCATCGT TTGCGGGACC GGGAGTTGAT TCTCTTTGCT 
GACTTGCCCT GCCAGTACCA TCGCTTGGAT GTCCCCCGGC CTTTTGTCTC CCGGGGCCCT 
CTAACTGTAG AGGAGACACC CAGGGTACAG GGAGGAACTC AACCCTCCAG GCTTCTGTTG 
GCTGAGGACT CAGAGGAAGA AGTAGATTCC CTTTTGGACA AGTGTGTGGT GAAAGGACCA 
AGGACCTCCT CTTCGGCAAC AGTCGTTCCA GAGAGTGATG AAGAAGGGCC TTCCCCTGCC 
CCAGATGGGC CCGGGCCACC TTCTGCCTTC AACTTGAACA GCGACACGGA TGAGGAAGAA 
AGTCAGGAAT CAGGAGCAGG GGAGGCCTCT TCAGCTGCCA GAAGAGGTAC CGCTGCAGAG 
ACGGAACAGC CTGAACCTGT CACAGCCGAG ATCCAGATTG AGAAGGATCA GTGTTCCGTG 
AAGGAGAGGA ACGGGGACAC AGAAATCGAG AGGGATGTGA GGAATGGGGT GGTTCCGACT 
GGAGTGATTC TGGAGAGGAG CCAGCCTTCT GGGGAGGACA GTGACACAGA TGTGAGTGAT 
GAGAGTGGGC CTCGAAGAAG GCTTGTTGGA GTCCGTCCGA AAAGGGCCTG GTCTTGTAAC 
TTCATAGACA GTGATACCGA TGGAGAGGAC GAGGGGATCC CTGCTACCCC AGCAGTGGTT 
CCCATGAAGG AGAGGCAGAT CTTCCACGAA GCTGGTACAC AGAGCCCCCA GGCACCTGGT 
GTGGCACGTT GGCAGGAGAG CCCAGCTGAT GGTGATACAG ATATAGAGGA GGGGGAGGCC 
CCCCCAGACA GAAGCCAAGC CTCCGTGGTG ATCGACAGCA ATACAGACGA TGAGGAAGAA 
GTCTCAGCAG CACTGACACT GGCATGTCTA AGAGAGAGCC AGGCTGGTAA GTGGAACCGA 
GATCCAGATG CAGAAGAGAC CAGGGCCCAA CCCGTGGCCC TTCTGGAGCG AAGCCAGGCC 
TCTGCTGGGG GAGACAGTGA CACAGATGTG GAGGAAGAGG GGCTCCCAGT GGAAAGGAGG 
GGAATGGTTC CCAAGGGTTA CATGGACAGG GAATATTCAA AAAAGAGCCA GCACCCTCCC 
AGGGACAGTG ATACAGAGGG GAAGGAAGAT GAGAGCTCAC CGGGGGTCCA CCTGGAGAGA 
AGCCAGGCCT CTGCACAAGT GGAGGATGAG GTCCCACTGG GGCCAGCTGT TGCACTTCCG 
GAGAAGCGTC AGGTACAAGG CATAGTGTGG CCACATCACA CCGATGCGGA GGCAGAAGGG 
GACCCGGCAC AGCTCCCCGT GCTGCGTCTA GAGGAAGCCC GGCCTCCTCT AGCTGGGGAC 
TGTGAACTGG ATTCGGAGAA CACATCTGCT GTCAGAAAGA GCCAGCTTCC GGCGGAAAAA 
GATGCTGGGA CCACGTGGGC TGCAGCCGTT CCTGAACAGG ACAGAGCACT TGCCATCGGG 
ACCCAGGGTG GGTCATCCAC GGCACCAGGG GAGCAAGACC TTCTCCCGGT CTCAAGGGAA 
GACCTAGCAG ATATGGTGGT GGACATAGGC ACTCCAGGGG AGCCCCAACC GCAGAGAGAG 
GGGGCCCAGA CCACCACAGG AAGGGAGAGA GAACCATATG GGAATAGGGC CACAGACTCT 
GGAGACAACC TCCACGATTC TGAAGATCTG GACCTACCAG CTACCCAGTG CTTTGTAGAC 
AGAAAGAATC AGAGCCTGGA AGCAGCCCCC AGCATGGAGG ATGAGCCCAC CCAGGCCTTC 
CTGTTTCCTC TGCCCCAAGA GCCTGGCCCT TCCTGTTGCA GCTCCCAGGC CACAGGTTCC 
TTGGATGAGT CATGGGAGGC CTTGGCGACA CAGCCATTCT GTCCGAGAGA ATCTGAGGCC 
TCCGAGACCC AAACCGTTGT CACCCTCCTT GACACCCATG CATCTTGGCC CTCTCCATCT 
AGGACAGCAC AGCAAGAGCA ACATCCAGAG AGCCCAGTCC ATGCAGAGCC ACTGGGGATG 
GAAGGCAGAG AGATGCAGAC TGTGGAGAAA GAAATGGGTA CCCCAAGAGA AACAGCAGAG 
AGGGTGATCC CTGAGGGAGG GCCACCGCAG GAGGAAACCA AGAAACTGCC CTCAGAAGGA 
GAGAGGGAAG ATGTGACGGG AGAGGAAGAA TTAATCGGGG GGATACAGGG CAGAGAAAAA 
AATCAGGTGT TTGCTAGAGA TACTCAGAGC CAAGAATCTG ACAAAAAAGT GAAAAGTGCA 
AGTACTGGAA GGGGAATGGA GATTTTAAAG GTAGAAATTG AGACACCCAA GGAAACACAA 
GAGAAAGAGA GAGAAAAGCA GACTCTCGCA GGGGAAATAT TTGAGAGTGA AGCAGGGAAA 
CTGGTAGTAG AGAGAGAGAG TGAGGCAGAT GGGTTAGAAG TCAAGGAACC CCAAGAGCTA 
CTGGACAGAA GCCCACAGAT GGGGGAGACA GAGGCGGGGG ACCAGGACCA GAAAGGCCAA 
GCCTCTGGTC TGCCATCAGA GCCTGGAGCA GGGGCAGGAG ACCTTCAGGG ACTTGCTTCA 
GACCCAGTAG CTTCTGGGAG CCAGGCAGGT GGAGGAAGGG GAGTCCCAGG GAGCCCCAGG 
AGGCAGCAGA GAGGTGACTT GAATTGCGAG ATGCCACCTG CTCAGAAGGC TTCCAGGGGT 
GATCAGGAAT CCCCAGATGC TTGTCTGCCT CCTGCAGCGC CTGAAGCCTC AGCCGCACTC 
CCAAATTCCC TCATCATCTC TCAGATCCAA AAACATCCCG CACCTCAGTC CCTCCTTTTT 
CCCTCTCTAG CTCCTTTAGA ACTGCCTATT CCCAGGACCA GACAAAATGA GACTCAGGAA 
GCTCCAGAGA CTCCCTTCTC CTCAGAGCTG AACTCTGTCC ACCCAGAACC CAAAGTCAGG 
CTCCAGGCGT CCTCTCCAGT TTCTTCTCTA CCCCTTGAGC CCCACCCTAC CACCCCCACA 
GGCCAGCCTA TTGCCCTTGA ACCCATATCT GGGGTCTCTT GGAGCAGGAC ACGTAGTTCC 
TTTGATGTAA CTGCCTCATC AGTTGTCCCC ACAGCCGTTG CACTGCAGCC ATCCATCTCC 
ACAGACAAGC CTGTCACCCC TAAGCCCACA CTTCGGGCCC CTCGGGGCAG GGCACAGAGG 
TCTTCTGTCA AAACCCCTGA ACCCAATGTC ACCACAGACC AGCCTGTTGC CCCTGAGCTC 
ACAGCTATGG CAACTCGGGG CAGGGCACAG AGGTCTTCTG TCAAGATTCC CAAACCAGAT 
AACCCCACAA CACCCAAGCC CCAGCCTTCC ACTTCCACAG ACCAGCCTGT CATCCCCAAA 
CCCACATCTC GGGCCCCTCG GGGCAGGACA CCTAGGTCTT CTGCCAAGAC TCCTGAACCA 
GCTGTCCCCA CAGCCTCTGA GCTCCAGCCT GCTGCCCCTA AAGACCAGCC TGTTGCTCCT 
GAGCTCACAT CTAGAGCCAC TCGGGGCAGG ACACAGAGGT CTATCAAGAC TTCCAAACCA 
GATATGCCCA CAACTCCCGA GCCCCAGCCT TCCACCTCCG CAGACCAGCC TGTCACCCCC 
AAACCCACGT CTCGGGCCCC TCGGGGCAGG ACACCTAGGT CTTCCGCCAA GACTCCTGAG 
CCAGTTGTCT CCACAGCCTC TGAGCTCCAG CCTTCTGCCC TCACAGATGA GCCTGTCACT 
CCTGAGCTCA CATCTAGGGC TACTCGGGGT CGGGCACAGA GGTCCTCTGT CAAAACCCCT 
GATCCAGTTA CCACCACAAC ACCTGAGCTC CAGCCTTCCA CCTCCACAGA CCAGCTTGTC 
ACCCCCAAAC GCACATCTCG GGCCCCTCGA GGCAGGACAC GTAGGTTGTC TGCCAAGACT 
CCTGAACCAG TTGTTCCCAC AGCCTCTGAG CTCCAGCCTT CTGCCCCTGC AGACCAGCCT 
GTTGGTCCTT GGGCCACTCA GTGTAGAAGA CATAGGTCTT CTGTCAAGAC CCCAGAACCA 
GTTGTCCCCA CAGTCCCTGA ACCTCAGCCT TCCACTTCTA AAGACCAGTC TGTCACTCCT 
GAGCCCACAT CTCAGGCCAC TGAGAGCCAA ACACATAGGT CCTCTATCAA GACATCCCAG 
CCAACGGAAC CCACAGCCTC TGACCTCAAA CCTTCCTCCC CCACAGACCA GCTTGTCACT 
CCCAAGGTCA TAGCTCAGGG TGGTCCAAGC AGGACAGGAA GGGCTTCCAC AGCAAGTGCT 
GTGCTGGTTC CTACTACCCC TGAATTCCAG TCTCCAGTCC CGTCAGAACA GCCTCTTCCC 
CCTGACCCCA TCCCTGAAGT CAACTGCAGC AGGAGGCTGA GGGCCACTAG GAAACATGGG 
TCTCCCACAG CTCATGTTCA TGAGCCCTGC ACCGTACCCC CTGAACCTAA CTCCCACTCT 
TCAAGGAACC AAAGACATGG GGCAGTGAAA GCAGCCAAGC CCCTTAGCAC CATTCCTGAG 
CCTGCCTTTG CCCAGCTCCC CGAGGCACCG CCTCACACTC CCCAGGTGCC AAAGGAGGAG 
GCAGCAGATG GATCAGGCTT CACCCCAGAG CCCCAGCCTA GGGCCTCTCA AAACCACAAG 
AGGCCTTCAG CTACTGCACA TTCACCTCCA CTTCAAAAAC GGCTCCAGAG AGGGAGAGTC 
CCTCAGAAGG CAGCATCCCT TAAGGAAGAA GAAAATCCAG TAGCGAGGCC GAGGAAGGAA 
GAGGGTGTAG TGATTCCAGG TCCAGGCAAG AGAAAGAGAG AGCAGACAGA GGAGGAGTCC 
CAGGGAAGAC CGAGCCGCAG CCTGCGACGG ACCAGACCGA TGCAGGAGTC CACGGCCCCC 
AAAGTGCTGT TCACCGGTGT GGTGGATGCT CGCGGAGAGA GGACGGTGCT GGCCCTGGGG 
GGCAGTTTGG CCAGCTCGGT GGCCGAGGCT TCTCACCTGG TGACTGATCG GATCCGCCGG 
ACGGTCAAGT TTCTGTGTGC CCTGGGCCGG GGCATCCCCA TCCTCTCCCT GGCCTGGCTG 
CATGAGTCCC GCAAGGCAGG CTGCTTCTTG CCCCCGGACG AATATTTGGT GACTGATCCT 
GAGCAGGAGA AGAACTTCGG CTTCAGCCTT CGGGAGGCCC TGAGCCGAGC TCAGGAGCGA 
AGGCTGCTGG AGGGCTATGA GATTCATGTG ACCCCCGGAG TCCAGCCACC GCCACCTCAG 
ATGGGAGAAA TCATCAGCTG CTGTGGAGGC GCCATCCTGC CCAGCATGCC TCGGTCCTAC 
AAGCCTCAGA GGTTCGTGAT CACATGTTCC CAGGACTTTC CTCGATGTGC TGTTCCATAT 
CGGGTTGGGC TGCCTATCCT CTCACCCGAA TTCCTGCTGA CGGGAGTACT GAAGCAGGAA 
GTCAAGCCAG AGGCCTTTGC CTTCTCCACT GTGGAAATGT CATCCACCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012019791.1
CDS	328..6138
Translation	MEVSHFPCLHFPFKIMEDTQILNWEVEEEEEVAESPNESLGYSLEPLAQLHIFSSSYGPEKDFPLYLGKNMIGRMPDCSVALPFSSISKQHAVIEISAWDKAPVLRDCGSLNGTQILRPPKVLSPGVSHRLRDRELILFADLPCQYHRLDVPRPFVSRGPLTVEETPRVQGGTQPSRLLLAEDSEEEVDSLLDKCVVKGPRTSSSATVVPESDEEGPSPAPDGPGPPSAFNLNSDTDEEESQESGAGEASSAARRGTAAETEQPEPVTAEIQIEKDQCSVKERNGDTEIERDVRNGVVPTGVILERSQPSGEDSDTDVSDESGPRRRLVGVRPKRAWSCNFIDSDTDGEDEGIPATPAVVPMKERQIFHEAGTQSPQAPGVARWQESPADGDTDIEEGEAPPDRSQASVVIDSNTDDEEEVSAALTLACLRESQAGKWNRDPDAEETRAQPVALLERSQASAGGDSDTDVEEEGLPVERRGMVPKGYMDREYSKKSQHPPRDSDTEGKEDESSPGVHLERSQASAQVEDEVPLGPAVALPEKRQVQGIVWPHHTDAEAEGDPAQLPVLRLEEARPPLAGDCELDSENTSAVRKSQLPAEKDAGTTWAAAVPEQDRALAIGTQGGSSTAPGEQDLLPVSREDLADMVVDIGTPGEPQPQREGAQTTTGREREPYGNRATDSGDNLHDSEDLDLPATQCFVDRKNQSLEAAPSMEDEPTQAFLFPLPQEPGPSCCSSQATGSLDESWEALATQPFCPRESEASETQTVVTLLDTHASWPSPSRTAQQEQHPESPVHAEPLGMEGREMQTVEKEMGTPRETAERVIPEGGPPQEETKKLPSEGEREDVTGEEELIGGIQGREKNQVFARDTQSQESDKKVKSASTGRGMEILKVEIETPKETQEKEREKQTLAGEIFESEAGKLVVERESEADGLEVKEPQELLDRSPQMGETEAGDQDQKGQASGLPSEPGAGAGDLQGLASDPVASGSQAGGGRGVPGSPRRQQRGDLNCEMPPAQKASRGDQESPDACLPPAAPEASAALPNSLIISQIQKHPAPQSLLFPSLAPLELPIPRTRQNETQEAPETPFSSELNSVHPEPKVRLQASSPVSSLPLEPHPTTPTGQPIALEPISGVSWSRTRSSFDVTASSVVPTAVALQPSISTDKPVTPKPTLRAPRGRAQRSSVKTPEPNVTTDQPVAPELTAMATRGRAQRSSVKIPKPDNPTTPKPQPSTSTDQPVIPKPTSRAPRGRTPRSSAKTPEPAVPTASELQPAAPKDQPVAPELTSRATRGRTQRSIKTSKPDMPTTPEPQPSTSADQPVTPKPTSRAPRGRTPRSSAKTPEPVVSTASELQPSALTDEPVTPELTSRATRGRAQRSSVKTPDPVTTTTPELQPSTSTDQLVTPKRTSRAPRGRTRRLSAKTPEPVVPTASELQPSAPADQPVGPWATQCRRHRSSVKTPEPVVPTVPEPQPSTSKDQSVTPEPTSQATESQTHRSSIKTSQPTEPTASDLKPSSPTDQLVTPKVIAQGGPSRTGRASTASAVLVPTTPEFQSPVPSEQPLPPDPIPEVNCSRRLRATRKHGSPTAHVHEPCTVPPEPNSHSSRNQRHGAVKAAKPLSTIPEPAFAQLPEAPPHTPQVPKEEAADGSGFTPEPQPRASQNHKRPSATAHSPPLQKRLQRGRVPQKAASLKEEENPVARPRKEEGVVIPGPGKRKREQTEEESQGRPSRSLRRTRPMQESTAPKVLFTGVVDARGERTVLALGGSLASSVAEASHLVTDRIRRTVKFLCALGRGIPILSLAWLHESRKAGCFLPPDEYLVTDPEQEKNFGFSLREALSRAQERRLLEGYEIHVTPGVQPPPPQMGEIISCCGGAILPSMPRSYKPQRFVITCSQDFPRCAVPYRVGLPILSPEFLLTGVLKQEVKPEAFAFSTVEMSST

Target ORF information:

RefSeq Version	XM_012164401.2
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon mediator of DNA damage checkpoint 1 (MDC1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012164401.2

ORF Insert Sequence:

ATGGAGGTGT CCCACTTTCC TTGTCTACAT TTTCCCTTTA AGATCATGGA AGACACCCAG 
ATTTTAAACT GGGAAGTTGA AGAAGAGGAG GAGGTTGCAG AGAGCCCCAA TGAATCTCTG 
GGGTATAGCT TGGAGCCCCT AGCACAGCTG CATATCTTCA GTAGTTCCTA TGGACCAGAA 
AAAGACTTCC CACTCTACCT TGGAAAGAAC ATGATAGGCC GAATGCCTGA TTGCTCTGTG 
GCCCTGCCCT TTTCATCAAT ATCCAAACAA CATGCAGTGA TTGAAATCTC TGCCTGGGAC 
AAGGCGCCTG TCCTTCGGGA TTGCGGGAGC CTCAATGGCA CTCAAATCCT GCGGCCTCCT 
AAGGTCCTGA GCCCTGGGGT GAGTCATCGT TTGCGGGACC GGGAGTTGAT TCTCTTTGCT 
GACTTGCCCT GCCAGTACCA TCGCTTGGAT GTCCCCCGGC CTTTTGTCTC CCGGGGCCCT 
CTAACTGTAG AGGAGACACC CAGGGTACAG GGAGGAACTC AACCCTCCAG GCTTCTGTTG 
GCTGAGGACT CAGAGGAAGA AGTAGATTCC CTTTTGGACA AGTGTGTGGT GAAAGGACCA 
AGGACCTCCT CTTCGGCAAC AGTCGTTCCA GAGAGTGATG AAGAAGGGCC TTCCCCTGCC 
CCAGATGGGC CCGGGCCACC TTCTGCCTTC AACTTGAACA GCGACACGGA TGAGGAAGAA 
AGTCAGGAAT CAGGAGCAGG GGAGGCCTCT TCAGCTGCCA GAAGAGGTAC CGCTGCAGAG 
ACGGAACAGC CTGAACCTGT CACAGCCGAG ATCCAGATTG AGAAGGATCA GTGTTCCGTG 
AAGGAGAGGA ACGGGGACAC AGAAATCGAG AGGGATGTGA GGAATGGGGT GGTTCCGACT 
GGAGTGATTC TGGAGAGGAG CCAGCCTTCT GGGGAGGACA GTGACACAGA TGTGAGTGAT 
GAGAGTGGGC CTCGAAGAAG GCTTGTTGGA GTCCGTCCGA AAAGGGCCTG GTCTTGTAAC 
TTCATAGACA GTGATACCGA TGGAGAGGAC GAGGGGATCC CTGCTACCCC AGCAGTGGTT 
CCCATGAAGG AGAGGCAGAT CTTCCACGAA GCTGGTACAC AGAGCCCCCA GGCACCTGGT 
GTGGCACGTT GGCAGGAGAG CCCAGCTGAT GGTGATACAG ATATAGAGGA GGGGGAGGCC 
CCCCCAGACA GAAGCCAAGC CTCCGTGGTG ATCGACAGCA ATACAGACGA TGAGGAAGAA 
GTCTCAGCAG CACTGACACT GGCATGTCTA AGAGAGAGCC AGGCTGGTAA GTGGAACCGA 
GATCCAGATG CAGAAGAGAC CAGGGCCCAA CCCGTGGCCC TTCTGGAGCG AAGCCAGGCC 
TCTGCTGGGG GAGACAGTGA CACAGATGTG GAGGAAGAGG GGCTCCCAGT GGAAAGGAGG 
GGAATGGTTC CCAAGGGTTA CATGGACAGG GAATATTCAA AAAAGAGCCA GCACCCTCCC 
AGGGACAGTG ATACAGAGGG GAAGGAAGAT GAGAGCTCAC CGGGGGTCCA CCTGGAGAGA 
AGCCAGGCCT CTGCACAAGT GGAGGATGAG GTCCCACTGG GGCCAGCTGT TGCACTTCCG 
GAGAAGCGTC AGGTACAAGG CATAGTGTGG CCACATCACA CCGATGCGGA GGCAGAAGGG 
GACCCGGCAC AGCTCCCCGT GCTGCGTCTA GAGGAAGCCC GGCCTCCTCT AGCTGGGGAC 
TGTGAACTGG ATTCGGAGAA CACATCTGCT GTCAGAAAGA GCCAGCTTCC GGCGGAAAAA 
GATGCTGGGA CCACGTGGGC TGCAGCCGTT CCTGAACAGG ACAGAGCACT TGCCATCGGG 
ACCCAGGGTG GGTCATCCAC GGCACCAGGG GAGCAAGACC TTCTCCCGGT CTCAAGGGAA 
GACCTAGCAG ATATGGTGGT GGACATAGGC ACTCCAGGGG AGCCCCAACC GCAGAGAGAG 
GGGGCCCAGA CCACCACAGG AAGGGAGAGA GAACCATATG GGAATAGGGC CACAGACTCT 
GGAGACAACC TCCACGATTC TGAAGATCTG GACCTACCAG CTACCCAGTG CTTTGTAGAC 
AGAAAGAATC AGAGCCTGGA AGCAGCCCCC AGCATGGAGG ATGAGCCCAC CCAGGCCTTC 
CTGTTTCCTC TGCCCCAAGA GCCTGGCCCT TCCTGTTGCA GCTCCCAGGC CACAGGTTCC 
TTGGATGAGT CATGGGAGGC CTTGGCGACA CAGCCATTCT GTCCGAGAGA ATCTGAGGCC 
TCCGAGACCC AAACCGTTGT CACCCTCCTT GACACCCATG CATCTTGGCC CTCTCCATCT 
AGGACAGCAC AGCAAGAGCA ACATCCAGAG AGCCCAGTCC ATGCAGAGCC ACTGGGGATG 
GAAGGCAGAG AGATGCAGAC TGTGGAGAAA GAAATGGGTA CCCCAAGAGA AACAGCAGAG 
AGGGTGATCC CTGAGGGAGG GCCACCGCAG GAGGAAACCA AGAAACTGCC CTCAGAAGGA 
GAGAGGGAAG ATGTGACGGG AGAGGAAGAA TTAATCGGGG GGATACAGGG CAGAGAAAAA 
AATCAGGTGT TTGCTAGAGA TACTCAGAGC CAAGAATCTG ACAAAAAAGT GAAAAGTGCA 
AGTACTGGAA GGGGAATGGA GATTTTAAAG GTAGAAATTG AGACACCCAA GGAAACACAA 
GAGAAAGAGA GAGAAAAGCA GACTCTCGCA GGGGAAATAT TTGAGAGTGA AGCAGGGAAA 
CTGGTAGTAG AGAGAGAGAG TGAGGCAGAT GGGTTAGAAG TCAAGGAACC CCAAGAGCTA 
CTGGACAGAA GCCCACAGAT GGGGGAGACA GAGGCGGGGG ACCAGGACCA GAAAGGCCAA 
GCCTCTGGTC TGCCATCAGA GCCTGGAGCA GGGGCAGGAG ACCTTCAGGG ACTTGCTTCA 
GACCCAGTAG CTTCTGGGAG CCAGGCAGGT GGAGGAAGGG GAGTCCCAGG GAGCCCCAGG 
AGGCAGCAGA GAGGTGACTT GAATTGCGAG ATGCCACCTG CTCAGAAGGC TTCCAGGGGT 
GATCAGGAAT CCCCAGATGC TTGTCTGCCT CCTGCAGCGC CTGAAGCCTC AGCCGCACTC 
CCAAATTCCC TCATCATCTC TCAGATCCAA AAACATCCCG CACCTCAGTC CCTCCTTTTT 
CCCTCTCTAG CTCCTTTAGA ACTGCCTATT CCCAGGACCA GACAAAATGA GACTCAGGAA 
GCTCCAGAGA CTCCCTTCTC CTCAGAGCTG AACTCTGTCC ACCCAGAACC CAAAGTCAGG 
CTCCAGGCGT CCTCTCCAGT TTCTTCTCTA CCCCTTGAGC CCCACCCTAC CACCCCCACA 
GGCCAGCCTA TTGCCCTTGA ACCCATATCT GGGGTCTCTT GGAGCAGGAC ACGTAGTTCC 
TTTGATGTAA CTGCCTCATC AGTTGTCCCC ACAGCCGTTG CACTGCAGCC ATCCATCTCC 
ACAGACAAGC CTGTCACCCC TAAGCCCACA CTTCGGGCCC CTCGGGGCAG GGCACAGAGG 
TCTTCTGTCA AAACCCCTGA ACCCAATGTC ACCACAGACC AGCCTGTTGC CCCTGAGCTC 
ACAGCTATGG CAACTCGGGG CAGGGCACAG AGGTCTTCTG TCAAGATTCC CAAACCAGAT 
AACCCCACAA CACCCAAGCC CCAGCCTTCC ACTTCCACAG ACCAGCCTGT CATCCCCAAA 
CCCACATCTC GGGCCCCTCG GGGCAGGACA CCTAGGTCTT CTGCCAAGAC TCCTGAACCA 
GCTGTCCCCA CAGCCTCTGA GCTCCAGCCT GCTGCCCCTA AAGACCAGCC TGTTGCTCCT 
GAGCTCACAT CTAGAGCCAC TCGGGGCAGG ACACAGAGGT CTATCAAGAC TTCCAAACCA 
GATATGCCCA CAACTCCCGA GCCCCAGCCT TCCACCTCCG CAGACCAGCC TGTCACCCCC 
AAACCCACGT CTCGGGCCCC TCGGGGCAGG ACACCTAGGT CTTCCGCCAA GACTCCTGAG 
CCAGTTGTCT CCACAGCCTC TGAGCTCCAG CCTTCTGCCC TCACAGATGA GCCTGTCACT 
CCTGAGCTCA CATCTAGGGC TACTCGGGGT CGGGCACAGA GGTCCTCTGT CAAAACCCCT 
GATCCAGTTA CCACCACAAC ACCTGAGCTC CAGCCTTCCA CCTCCACAGA CCAGCTTGTC 
ACCCCCAAAC GCACATCTCG GGCCCCTCGA GGCAGGACAC GTAGGTTGTC TGCCAAGACT 
CCTGAACCAG TTGTTCCCAC AGCCTCTGAG CTCCAGCCTT CTGCCCCTGC AGACCAGCCT 
GTTGGTCCTT GGGCCACTCA GTGTAGAAGA CATAGGTCTT CTGTCAAGAC CCCAGAACCA 
GTTGTCCCCA CAGTCCCTGA ACCTCAGCCT TCCACTTCTA AAGACCAGTC TGTCACTCCT 
GAGCCCACAT CTCAGGCCAC TGAGAGCCAA ACACATAGGT CCTCTATCAA GACATCCCAG 
CCAACGGAAC CCACAGCCTC TGACCTCAAA CCTTCCTCCC CCACAGACCA GCTTGTCACT 
CCCAAGGTCA TAGCTCAGGG TGGTCCAAGC AGGACAGGAA GGGCTTCCAC AGCAAGTGCT 
GTGCTGGTTC CTACTACCCC TGAATTCCAG TCTCCAGTCC CGTCAGAACA GCCTCTTCCC 
CCTGACCCCA TCCCTGAAGT CAACTGCAGC AGGAGGCTGA GGGCCACTAG GAAACATGGG 
TCTCCCACAG CTCATGTTCA TGAGCCCTGC ACCGTACCCC CTGAACCTAA CTCCCACTCT 
TCAAGGAACC AAAGACATGG GGCAGTGAAA GCAGCCAAGC CCCTTAGCAC CATTCCTGAG 
CCTGCCTTTG CCCAGCTCCC CGAGGCACCG CCTCACACTC CCCAGGTGCC AAAGGAGGAG 
GCAGCAGATG GATCAGGCTT CACCCCAGAG CCCCAGCCTA GGGCCTCTCA AAACCACAAG 
AGGCCTTCAG CTACTGCACA TTCACCTCCA CTTCAAAAAC GGCTCCAGAG AGGGAGAGTC 
CCTCAGAAGG CAGCATCCCT TAAGGAAGAA GAAAATCCAG TAGCGAGGCC GAGGAAGGAA 
GAGGGTGTAG TGATTCCAGG TCCAGGCAAG AGAAAGAGAG AGCAGACAGA GGAGGAGTCC 
CAGGGAAGAC CGAGCCGCAG CCTGCGACGG ACCAGACCGA TGCAGGAGTC CACGGCCCCC 
AAAGTGCTGT TCACCGGTGT GGTGGATGCT CGCGGAGAGA GGACGGTGCT GGCCCTGGGG 
GGCAGTTTGG CCAGCTCGGT GGCCGAGGCT TCTCACCTGG TGACTGATCG GATCCGCCGG 
ACGGTCAAGT TTCTGTGTGC CCTGGGCCGG GGCATCCCCA TCCTCTCCCT GGCCTGGCTG 
CATGAGTCCC GCAAGGCAGG CTGCTTCTTG CCCCCGGACG AATATTTGGT GACTGATCCT 
GAGCAGGAGA AGAACTTCGG CTTCAGCCTT CGGGAGGCCC TGAGCCGAGC TCAGGAGCGA 
AGGCTGCTGG AGGGCTATGA GATTCATGTG ACCCCCGGAG TCCAGCCACC GCCACCTCAG 
ATGGGAGAAA TCATCAGCTG CTGTGGAGGC GCCATCCTGC CCAGCATGCC TCGGTCCTAC 
AAGCCTCAGA GGTTCGTGAT CACATGTTCC CAGGACTTTC CTCGATGTGC TGTTCCATAT 
CGGGTTGGGC TGCCTATCCT CTCACCCGAA TTCCTGCTGA CGGGAGTACT GAAGCAGGAA 
GTCAAGCCAG AGGCCTTTGC CTTCTCCACT GTGGAAATGT CATCCACCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MDC1 ( XM_012164402.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_012019792.1 Ovis aries musimon mediator of DNA damage checkpoint 1 (MDC1), transcript variant X10, mRNA.

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CloneID	OOv35148
Clone ID Related Accession (Same CDS sequence)	XM_015090177.1 , XM_012164401.2 , XM_012164402.2
Accession Version	XM_012164402.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5811bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	mediator of DNA damage checkpoint protein 1 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011943095.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012164402.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGTGT CCCACTTTCC TTGTCTACAT TTTCCCTTTA AGATCATGGA AGACACCCAG 
ATTTTAAACT GGGAAGTTGA AGAAGAGGAG GAGGTTGCAG AGAGCCCCAA TGAATCTCTG 
GGGTATAGCT TGGAGCCCCT AGCACAGCTG CATATCTTCA GTAGTTCCTA TGGACCAGAA 
AAAGACTTCC CACTCTACCT TGGAAAGAAC ATGATAGGCC GAATGCCTGA TTGCTCTGTG 
GCCCTGCCCT TTTCATCAAT ATCCAAACAA CATGCAGTGA TTGAAATCTC TGCCTGGGAC 
AAGGCGCCTG TCCTTCGGGA TTGCGGGAGC CTCAATGGCA CTCAAATCCT GCGGCCTCCT 
AAGGTCCTGA GCCCTGGGGT GAGTCATCGT TTGCGGGACC GGGAGTTGAT TCTCTTTGCT 
GACTTGCCCT GCCAGTACCA TCGCTTGGAT GTCCCCCGGC CTTTTGTCTC CCGGGGCCCT 
CTAACTGTAG AGGAGACACC CAGGGTACAG GGAGGAACTC AACCCTCCAG GCTTCTGTTG 
GCTGAGGACT CAGAGGAAGA AGTAGATTCC CTTTTGGACA AGTGTGTGGT GAAAGGACCA 
AGGACCTCCT CTTCGGCAAC AGTCGTTCCA GAGAGTGATG AAGAAGGGCC TTCCCCTGCC 
CCAGATGGGC CCGGGCCACC TTCTGCCTTC AACTTGAACA GCGACACGGA TGAGGAAGAA 
AGTCAGGAAT CAGGAGCAGG GGAGGCCTCT TCAGCTGCCA GAAGAGGTAC CGCTGCAGAG 
ACGGAACAGC CTGAACCTGT CACAGCCGAG ATCCAGATTG AGAAGGATCA GTGTTCCGTG 
AAGGAGAGGA ACGGGGACAC AGAAATCGAG AGGGATGTGA GGAATGGGGT GGTTCCGACT 
GGAGTGATTC TGGAGAGGAG CCAGCCTTCT GGGGAGGACA GTGACACAGA TGTGAGTGAT 
GAGAGTGGGC CTCGAAGAAG GCTTGTTGGA GTCCGTCCGA AAAGGGCCTG GTCTTGTAAC 
TTCATAGACA GTGATACCGA TGGAGAGGAC GAGGGGATCC CTGCTACCCC AGCAGTGGTT 
CCCATGAAGG AGAGGCAGAT CTTCCACGAA GCTGGTACAC AGAGCCCCCA GGCACCTGGT 
GTGGCACGTT GGCAGGAGAG CCCAGCTGAT GGTGATACAG ATATAGAGGA GGGGGAGGCC 
CCCCCAGACA GAAGCCAAGC CTCCGTGGTG ATCGACAGCA ATACAGACGA TGAGGAAGAA 
GTCTCAGCAG CACTGACACT GGCATGTCTA AGAGAGAGCC AGGCTGGTAA GTGGAACCGA 
GATCCAGATG CAGAAGAGAC CAGGGCCCAA CCCGTGGCCC TTCTGGAGCG AAGCCAGGCC 
TCTGCTGGGG GAGACAGTGA CACAGATGTG GAGGAAGAGG GGCTCCCAGT GGAAAGGAGG 
GGAATGGTTC CCAAGGGTTA CATGGACAGG GAATATTCAA AAAAGAGCCA GCACCCTCCC 
AGGGACAGTG ATACAGAGGG GAAGGAAGAT GAGAGCTCAC CGGGGGTCCA CCTGGAGAGA 
AGCCAGGCCT CTGCACAAGT GGAGGATGAG GTCCCACTGG GGCCAGCTGT TGCACTTCCG 
GAGAAGCGTC AGGTACAAGG CATAGTGTGG CCACATCACA CCGATGCGGA GGCAGAAGGG 
GACCCGGCAC AGCTCCCCGT GCTGCGTCTA GAGGAAGCCC GGCCTCCTCT AGCTGGGGAC 
TGTGAACTGG ATTCGGAGAA CACATCTGCT GTCAGAAAGA GCCAGCTTCC GGCGGAAAAA 
GATGCTGGGA CCACGTGGGC TGCAGCCGTT CCTGAACAGG ACAGAGCACT TGCCATCGGG 
ACCCAGGGTG GGTCATCCAC GGCACCAGGG GAGCAAGACC TTCTCCCGGT CTCAAGGGAA 
GACCTAGCAG ATATGGTGGT GGACATAGGC ACTCCAGGGG AGCCCCAACC GCAGAGAGAG 
GGGGCCCAGA CCACCACAGG AAGGGAGAGA GAACCATATG GGAATAGGGC CACAGACTCT 
GGAGACAACC TCCACGATTC TGAAGATCTG GACCTACCAG CTACCCAGTG CTTTGTAGAC 
AGAAAGAATC AGAGCCTGGA AGCAGCCCCC AGCATGGAGG ATGAGCCCAC CCAGGCCTTC 
CTGTTTCCTC TGCCCCAAGA GCCTGGCCCT TCCTGTTGCA GCTCCCAGGC CACAGGTTCC 
TTGGATGAGT CATGGGAGGC CTTGGCGACA CAGCCATTCT GTCCGAGAGA ATCTGAGGCC 
TCCGAGACCC AAACCGTTGT CACCCTCCTT GACACCCATG CATCTTGGCC CTCTCCATCT 
AGGACAGCAC AGCAAGAGCA ACATCCAGAG AGCCCAGTCC ATGCAGAGCC ACTGGGGATG 
GAAGGCAGAG AGATGCAGAC TGTGGAGAAA GAAATGGGTA CCCCAAGAGA AACAGCAGAG 
AGGGTGATCC CTGAGGGAGG GCCACCGCAG GAGGAAACCA AGAAACTGCC CTCAGAAGGA 
GAGAGGGAAG ATGTGACGGG AGAGGAAGAA TTAATCGGGG GGATACAGGG CAGAGAAAAA 
AATCAGGTGT TTGCTAGAGA TACTCAGAGC CAAGAATCTG ACAAAAAAGT GAAAAGTGCA 
AGTACTGGAA GGGGAATGGA GATTTTAAAG GTAGAAATTG AGACACCCAA GGAAACACAA 
GAGAAAGAGA GAGAAAAGCA GACTCTCGCA GGGGAAATAT TTGAGAGTGA AGCAGGGAAA 
CTGGTAGTAG AGAGAGAGAG TGAGGCAGAT GGGTTAGAAG TCAAGGAACC CCAAGAGCTA 
CTGGACAGAA GCCCACAGAT GGGGGAGACA GAGGCGGGGG ACCAGGACCA GAAAGGCCAA 
GCCTCTGGTC TGCCATCAGA GCCTGGAGCA GGGGCAGGAG ACCTTCAGGG ACTTGCTTCA 
GACCCAGTAG CTTCTGGGAG CCAGGCAGGT GGAGGAAGGG GAGTCCCAGG GAGCCCCAGG 
AGGCAGCAGA GAGGTGACTT GAATTGCGAG ATGCCACCTG CTCAGAAGGC TTCCAGGGGT 
GATCAGGAAT CCCCAGATGC TTGTCTGCCT CCTGCAGCGC CTGAAGCCTC AGCCGCACTC 
CCAAATTCCC TCATCATCTC TCAGATCCAA AAACATCCCG CACCTCAGTC CCTCCTTTTT 
CCCTCTCTAG CTCCTTTAGA ACTGCCTATT CCCAGGACCA GACAAAATGA GACTCAGGAA 
GCTCCAGAGA CTCCCTTCTC CTCAGAGCTG AACTCTGTCC ACCCAGAACC CAAAGTCAGG 
CTCCAGGCGT CCTCTCCAGT TTCTTCTCTA CCCCTTGAGC CCCACCCTAC CACCCCCACA 
GGCCAGCCTA TTGCCCTTGA ACCCATATCT GGGGTCTCTT GGAGCAGGAC ACGTAGTTCC 
TTTGATGTAA CTGCCTCATC AGTTGTCCCC ACAGCCGTTG CACTGCAGCC ATCCATCTCC 
ACAGACAAGC CTGTCACCCC TAAGCCCACA CTTCGGGCCC CTCGGGGCAG GGCACAGAGG 
TCTTCTGTCA AAACCCCTGA ACCCAATGTC ACCACAGACC AGCCTGTTGC CCCTGAGCTC 
ACAGCTATGG CAACTCGGGG CAGGGCACAG AGGTCTTCTG TCAAGATTCC CAAACCAGAT 
AACCCCACAA CACCCAAGCC CCAGCCTTCC ACTTCCACAG ACCAGCCTGT CATCCCCAAA 
CCCACATCTC GGGCCCCTCG GGGCAGGACA CCTAGGTCTT CTGCCAAGAC TCCTGAACCA 
GCTGTCCCCA CAGCCTCTGA GCTCCAGCCT GCTGCCCCTA AAGACCAGCC TGTTGCTCCT 
GAGCTCACAT CTAGAGCCAC TCGGGGCAGG ACACAGAGGT CTATCAAGAC TTCCAAACCA 
GATATGCCCA CAACTCCCGA GCCCCAGCCT TCCACCTCCG CAGACCAGCC TGTCACCCCC 
AAACCCACGT CTCGGGCCCC TCGGGGCAGG ACACCTAGGT CTTCCGCCAA GACTCCTGAG 
CCAGTTGTCT CCACAGCCTC TGAGCTCCAG CCTTCTGCCC TCACAGATGA GCCTGTCACT 
CCTGAGCTCA CATCTAGGGC TACTCGGGGT CGGGCACAGA GGTCCTCTGT CAAAACCCCT 
GATCCAGTTA CCACCACAAC ACCTGAGCTC CAGCCTTCCA CCTCCACAGA CCAGCTTGTC 
ACCCCCAAAC GCACATCTCG GGCCCCTCGA GGCAGGACAC GTAGGTTGTC TGCCAAGACT 
CCTGAACCAG TTGTTCCCAC AGCCTCTGAG CTCCAGCCTT CTGCCCCTGC AGACCAGCCT 
GTTGGTCCTT GGGCCACTCA GTGTAGAAGA CATAGGTCTT CTGTCAAGAC CCCAGAACCA 
GTTGTCCCCA CAGTCCCTGA ACCTCAGCCT TCCACTTCTA AAGACCAGTC TGTCACTCCT 
GAGCCCACAT CTCAGGCCAC TGAGAGCCAA ACACATAGGT CCTCTATCAA GACATCCCAG 
CCAACGGAAC CCACAGCCTC TGACCTCAAA CCTTCCTCCC CCACAGACCA GCTTGTCACT 
CCCAAGGTCA TAGCTCAGGG TGGTCCAAGC AGGACAGGAA GGGCTTCCAC AGCAAGTGCT 
GTGCTGGTTC CTACTACCCC TGAATTCCAG TCTCCAGTCC CGTCAGAACA GCCTCTTCCC 
CCTGACCCCA TCCCTGAAGT CAACTGCAGC AGGAGGCTGA GGGCCACTAG GAAACATGGG 
TCTCCCACAG CTCATGTTCA TGAGCCCTGC ACCGTACCCC CTGAACCTAA CTCCCACTCT 
TCAAGGAACC AAAGACATGG GGCAGTGAAA GCAGCCAAGC CCCTTAGCAC CATTCCTGAG 
CCTGCCTTTG CCCAGCTCCC CGAGGCACCG CCTCACACTC CCCAGGTGCC AAAGGAGGAG 
GCAGCAGATG GATCAGGCTT CACCCCAGAG CCCCAGCCTA GGGCCTCTCA AAACCACAAG 
AGGCCTTCAG CTACTGCACA TTCACCTCCA CTTCAAAAAC GGCTCCAGAG AGGGAGAGTC 
CCTCAGAAGG CAGCATCCCT TAAGGAAGAA GAAAATCCAG TAGCGAGGCC GAGGAAGGAA 
GAGGGTGTAG TGATTCCAGG TCCAGGCAAG AGAAAGAGAG AGCAGACAGA GGAGGAGTCC 
CAGGGAAGAC CGAGCCGCAG CCTGCGACGG ACCAGACCGA TGCAGGAGTC CACGGCCCCC 
AAAGTGCTGT TCACCGGTGT GGTGGATGCT CGCGGAGAGA GGACGGTGCT GGCCCTGGGG 
GGCAGTTTGG CCAGCTCGGT GGCCGAGGCT TCTCACCTGG TGACTGATCG GATCCGCCGG 
ACGGTCAAGT TTCTGTGTGC CCTGGGCCGG GGCATCCCCA TCCTCTCCCT GGCCTGGCTG 
CATGAGTCCC GCAAGGCAGG CTGCTTCTTG CCCCCGGACG AATATTTGGT GACTGATCCT 
GAGCAGGAGA AGAACTTCGG CTTCAGCCTT CGGGAGGCCC TGAGCCGAGC TCAGGAGCGA 
AGGCTGCTGG AGGGCTATGA GATTCATGTG ACCCCCGGAG TCCAGCCACC GCCACCTCAG 
ATGGGAGAAA TCATCAGCTG CTGTGGAGGC GCCATCCTGC CCAGCATGCC TCGGTCCTAC 
AAGCCTCAGA GGTTCGTGAT CACATGTTCC CAGGACTTTC CTCGATGTGC TGTTCCATAT 
CGGGTTGGGC TGCCTATCCT CTCACCCGAA TTCCTGCTGA CGGGAGTACT GAAGCAGGAA 
GTCAAGCCAG AGGCCTTTGC CTTCTCCACT GTGGAAATGT CATCCACCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012019792.1
CDS	460..6270
Translation	MEVSHFPCLHFPFKIMEDTQILNWEVEEEEEVAESPNESLGYSLEPLAQLHIFSSSYGPEKDFPLYLGKNMIGRMPDCSVALPFSSISKQHAVIEISAWDKAPVLRDCGSLNGTQILRPPKVLSPGVSHRLRDRELILFADLPCQYHRLDVPRPFVSRGPLTVEETPRVQGGTQPSRLLLAEDSEEEVDSLLDKCVVKGPRTSSSATVVPESDEEGPSPAPDGPGPPSAFNLNSDTDEEESQESGAGEASSAARRGTAAETEQPEPVTAEIQIEKDQCSVKERNGDTEIERDVRNGVVPTGVILERSQPSGEDSDTDVSDESGPRRRLVGVRPKRAWSCNFIDSDTDGEDEGIPATPAVVPMKERQIFHEAGTQSPQAPGVARWQESPADGDTDIEEGEAPPDRSQASVVIDSNTDDEEEVSAALTLACLRESQAGKWNRDPDAEETRAQPVALLERSQASAGGDSDTDVEEEGLPVERRGMVPKGYMDREYSKKSQHPPRDSDTEGKEDESSPGVHLERSQASAQVEDEVPLGPAVALPEKRQVQGIVWPHHTDAEAEGDPAQLPVLRLEEARPPLAGDCELDSENTSAVRKSQLPAEKDAGTTWAAAVPEQDRALAIGTQGGSSTAPGEQDLLPVSREDLADMVVDIGTPGEPQPQREGAQTTTGREREPYGNRATDSGDNLHDSEDLDLPATQCFVDRKNQSLEAAPSMEDEPTQAFLFPLPQEPGPSCCSSQATGSLDESWEALATQPFCPRESEASETQTVVTLLDTHASWPSPSRTAQQEQHPESPVHAEPLGMEGREMQTVEKEMGTPRETAERVIPEGGPPQEETKKLPSEGEREDVTGEEELIGGIQGREKNQVFARDTQSQESDKKVKSASTGRGMEILKVEIETPKETQEKEREKQTLAGEIFESEAGKLVVERESEADGLEVKEPQELLDRSPQMGETEAGDQDQKGQASGLPSEPGAGAGDLQGLASDPVASGSQAGGGRGVPGSPRRQQRGDLNCEMPPAQKASRGDQESPDACLPPAAPEASAALPNSLIISQIQKHPAPQSLLFPSLAPLELPIPRTRQNETQEAPETPFSSELNSVHPEPKVRLQASSPVSSLPLEPHPTTPTGQPIALEPISGVSWSRTRSSFDVTASSVVPTAVALQPSISTDKPVTPKPTLRAPRGRAQRSSVKTPEPNVTTDQPVAPELTAMATRGRAQRSSVKIPKPDNPTTPKPQPSTSTDQPVIPKPTSRAPRGRTPRSSAKTPEPAVPTASELQPAAPKDQPVAPELTSRATRGRTQRSIKTSKPDMPTTPEPQPSTSADQPVTPKPTSRAPRGRTPRSSAKTPEPVVSTASELQPSALTDEPVTPELTSRATRGRAQRSSVKTPDPVTTTTPELQPSTSTDQLVTPKRTSRAPRGRTRRLSAKTPEPVVPTASELQPSAPADQPVGPWATQCRRHRSSVKTPEPVVPTVPEPQPSTSKDQSVTPEPTSQATESQTHRSSIKTSQPTEPTASDLKPSSPTDQLVTPKVIAQGGPSRTGRASTASAVLVPTTPEFQSPVPSEQPLPPDPIPEVNCSRRLRATRKHGSPTAHVHEPCTVPPEPNSHSSRNQRHGAVKAAKPLSTIPEPAFAQLPEAPPHTPQVPKEEAADGSGFTPEPQPRASQNHKRPSATAHSPPLQKRLQRGRVPQKAASLKEEENPVARPRKEEGVVIPGPGKRKREQTEEESQGRPSRSLRRTRPMQESTAPKVLFTGVVDARGERTVLALGGSLASSVAEASHLVTDRIRRTVKFLCALGRGIPILSLAWLHESRKAGCFLPPDEYLVTDPEQEKNFGFSLREALSRAQERRLLEGYEIHVTPGVQPPPPQMGEIISCCGGAILPSMPRSYKPQRFVITCSQDFPRCAVPYRVGLPILSPEFLLTGVLKQEVKPEAFAFSTVEMSST

Target ORF information:

RefSeq Version	XM_012164402.2
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon mediator of DNA damage checkpoint 1 (MDC1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012164402.2

ORF Insert Sequence:

ATGGAGGTGT CCCACTTTCC TTGTCTACAT TTTCCCTTTA AGATCATGGA AGACACCCAG 
ATTTTAAACT GGGAAGTTGA AGAAGAGGAG GAGGTTGCAG AGAGCCCCAA TGAATCTCTG 
GGGTATAGCT TGGAGCCCCT AGCACAGCTG CATATCTTCA GTAGTTCCTA TGGACCAGAA 
AAAGACTTCC CACTCTACCT TGGAAAGAAC ATGATAGGCC GAATGCCTGA TTGCTCTGTG 
GCCCTGCCCT TTTCATCAAT ATCCAAACAA CATGCAGTGA TTGAAATCTC TGCCTGGGAC 
AAGGCGCCTG TCCTTCGGGA TTGCGGGAGC CTCAATGGCA CTCAAATCCT GCGGCCTCCT 
AAGGTCCTGA GCCCTGGGGT GAGTCATCGT TTGCGGGACC GGGAGTTGAT TCTCTTTGCT 
GACTTGCCCT GCCAGTACCA TCGCTTGGAT GTCCCCCGGC CTTTTGTCTC CCGGGGCCCT 
CTAACTGTAG AGGAGACACC CAGGGTACAG GGAGGAACTC AACCCTCCAG GCTTCTGTTG 
GCTGAGGACT CAGAGGAAGA AGTAGATTCC CTTTTGGACA AGTGTGTGGT GAAAGGACCA 
AGGACCTCCT CTTCGGCAAC AGTCGTTCCA GAGAGTGATG AAGAAGGGCC TTCCCCTGCC 
CCAGATGGGC CCGGGCCACC TTCTGCCTTC AACTTGAACA GCGACACGGA TGAGGAAGAA 
AGTCAGGAAT CAGGAGCAGG GGAGGCCTCT TCAGCTGCCA GAAGAGGTAC CGCTGCAGAG 
ACGGAACAGC CTGAACCTGT CACAGCCGAG ATCCAGATTG AGAAGGATCA GTGTTCCGTG 
AAGGAGAGGA ACGGGGACAC AGAAATCGAG AGGGATGTGA GGAATGGGGT GGTTCCGACT 
GGAGTGATTC TGGAGAGGAG CCAGCCTTCT GGGGAGGACA GTGACACAGA TGTGAGTGAT 
GAGAGTGGGC CTCGAAGAAG GCTTGTTGGA GTCCGTCCGA AAAGGGCCTG GTCTTGTAAC 
TTCATAGACA GTGATACCGA TGGAGAGGAC GAGGGGATCC CTGCTACCCC AGCAGTGGTT 
CCCATGAAGG AGAGGCAGAT CTTCCACGAA GCTGGTACAC AGAGCCCCCA GGCACCTGGT 
GTGGCACGTT GGCAGGAGAG CCCAGCTGAT GGTGATACAG ATATAGAGGA GGGGGAGGCC 
CCCCCAGACA GAAGCCAAGC CTCCGTGGTG ATCGACAGCA ATACAGACGA TGAGGAAGAA 
GTCTCAGCAG CACTGACACT GGCATGTCTA AGAGAGAGCC AGGCTGGTAA GTGGAACCGA 
GATCCAGATG CAGAAGAGAC CAGGGCCCAA CCCGTGGCCC TTCTGGAGCG AAGCCAGGCC 
TCTGCTGGGG GAGACAGTGA CACAGATGTG GAGGAAGAGG GGCTCCCAGT GGAAAGGAGG 
GGAATGGTTC CCAAGGGTTA CATGGACAGG GAATATTCAA AAAAGAGCCA GCACCCTCCC 
AGGGACAGTG ATACAGAGGG GAAGGAAGAT GAGAGCTCAC CGGGGGTCCA CCTGGAGAGA 
AGCCAGGCCT CTGCACAAGT GGAGGATGAG GTCCCACTGG GGCCAGCTGT TGCACTTCCG 
GAGAAGCGTC AGGTACAAGG CATAGTGTGG CCACATCACA CCGATGCGGA GGCAGAAGGG 
GACCCGGCAC AGCTCCCCGT GCTGCGTCTA GAGGAAGCCC GGCCTCCTCT AGCTGGGGAC 
TGTGAACTGG ATTCGGAGAA CACATCTGCT GTCAGAAAGA GCCAGCTTCC GGCGGAAAAA 
GATGCTGGGA CCACGTGGGC TGCAGCCGTT CCTGAACAGG ACAGAGCACT TGCCATCGGG 
ACCCAGGGTG GGTCATCCAC GGCACCAGGG GAGCAAGACC TTCTCCCGGT CTCAAGGGAA 
GACCTAGCAG ATATGGTGGT GGACATAGGC ACTCCAGGGG AGCCCCAACC GCAGAGAGAG 
GGGGCCCAGA CCACCACAGG AAGGGAGAGA GAACCATATG GGAATAGGGC CACAGACTCT 
GGAGACAACC TCCACGATTC TGAAGATCTG GACCTACCAG CTACCCAGTG CTTTGTAGAC 
AGAAAGAATC AGAGCCTGGA AGCAGCCCCC AGCATGGAGG ATGAGCCCAC CCAGGCCTTC 
CTGTTTCCTC TGCCCCAAGA GCCTGGCCCT TCCTGTTGCA GCTCCCAGGC CACAGGTTCC 
TTGGATGAGT CATGGGAGGC CTTGGCGACA CAGCCATTCT GTCCGAGAGA ATCTGAGGCC 
TCCGAGACCC AAACCGTTGT CACCCTCCTT GACACCCATG CATCTTGGCC CTCTCCATCT 
AGGACAGCAC AGCAAGAGCA ACATCCAGAG AGCCCAGTCC ATGCAGAGCC ACTGGGGATG 
GAAGGCAGAG AGATGCAGAC TGTGGAGAAA GAAATGGGTA CCCCAAGAGA AACAGCAGAG 
AGGGTGATCC CTGAGGGAGG GCCACCGCAG GAGGAAACCA AGAAACTGCC CTCAGAAGGA 
GAGAGGGAAG ATGTGACGGG AGAGGAAGAA TTAATCGGGG GGATACAGGG CAGAGAAAAA 
AATCAGGTGT TTGCTAGAGA TACTCAGAGC CAAGAATCTG ACAAAAAAGT GAAAAGTGCA 
AGTACTGGAA GGGGAATGGA GATTTTAAAG GTAGAAATTG AGACACCCAA GGAAACACAA 
GAGAAAGAGA GAGAAAAGCA GACTCTCGCA GGGGAAATAT TTGAGAGTGA AGCAGGGAAA 
CTGGTAGTAG AGAGAGAGAG TGAGGCAGAT GGGTTAGAAG TCAAGGAACC CCAAGAGCTA 
CTGGACAGAA GCCCACAGAT GGGGGAGACA GAGGCGGGGG ACCAGGACCA GAAAGGCCAA 
GCCTCTGGTC TGCCATCAGA GCCTGGAGCA GGGGCAGGAG ACCTTCAGGG ACTTGCTTCA 
GACCCAGTAG CTTCTGGGAG CCAGGCAGGT GGAGGAAGGG GAGTCCCAGG GAGCCCCAGG 
AGGCAGCAGA GAGGTGACTT GAATTGCGAG ATGCCACCTG CTCAGAAGGC TTCCAGGGGT 
GATCAGGAAT CCCCAGATGC TTGTCTGCCT CCTGCAGCGC CTGAAGCCTC AGCCGCACTC 
CCAAATTCCC TCATCATCTC TCAGATCCAA AAACATCCCG CACCTCAGTC CCTCCTTTTT 
CCCTCTCTAG CTCCTTTAGA ACTGCCTATT CCCAGGACCA GACAAAATGA GACTCAGGAA 
GCTCCAGAGA CTCCCTTCTC CTCAGAGCTG AACTCTGTCC ACCCAGAACC CAAAGTCAGG 
CTCCAGGCGT CCTCTCCAGT TTCTTCTCTA CCCCTTGAGC CCCACCCTAC CACCCCCACA 
GGCCAGCCTA TTGCCCTTGA ACCCATATCT GGGGTCTCTT GGAGCAGGAC ACGTAGTTCC 
TTTGATGTAA CTGCCTCATC AGTTGTCCCC ACAGCCGTTG CACTGCAGCC ATCCATCTCC 
ACAGACAAGC CTGTCACCCC TAAGCCCACA CTTCGGGCCC CTCGGGGCAG GGCACAGAGG 
TCTTCTGTCA AAACCCCTGA ACCCAATGTC ACCACAGACC AGCCTGTTGC CCCTGAGCTC 
ACAGCTATGG CAACTCGGGG CAGGGCACAG AGGTCTTCTG TCAAGATTCC CAAACCAGAT 
AACCCCACAA CACCCAAGCC CCAGCCTTCC ACTTCCACAG ACCAGCCTGT CATCCCCAAA 
CCCACATCTC GGGCCCCTCG GGGCAGGACA CCTAGGTCTT CTGCCAAGAC TCCTGAACCA 
GCTGTCCCCA CAGCCTCTGA GCTCCAGCCT GCTGCCCCTA AAGACCAGCC TGTTGCTCCT 
GAGCTCACAT CTAGAGCCAC TCGGGGCAGG ACACAGAGGT CTATCAAGAC TTCCAAACCA 
GATATGCCCA CAACTCCCGA GCCCCAGCCT TCCACCTCCG CAGACCAGCC TGTCACCCCC 
AAACCCACGT CTCGGGCCCC TCGGGGCAGG ACACCTAGGT CTTCCGCCAA GACTCCTGAG 
CCAGTTGTCT CCACAGCCTC TGAGCTCCAG CCTTCTGCCC TCACAGATGA GCCTGTCACT 
CCTGAGCTCA CATCTAGGGC TACTCGGGGT CGGGCACAGA GGTCCTCTGT CAAAACCCCT 
GATCCAGTTA CCACCACAAC ACCTGAGCTC CAGCCTTCCA CCTCCACAGA CCAGCTTGTC 
ACCCCCAAAC GCACATCTCG GGCCCCTCGA GGCAGGACAC GTAGGTTGTC TGCCAAGACT 
CCTGAACCAG TTGTTCCCAC AGCCTCTGAG CTCCAGCCTT CTGCCCCTGC AGACCAGCCT 
GTTGGTCCTT GGGCCACTCA GTGTAGAAGA CATAGGTCTT CTGTCAAGAC CCCAGAACCA 
GTTGTCCCCA CAGTCCCTGA ACCTCAGCCT TCCACTTCTA AAGACCAGTC TGTCACTCCT 
GAGCCCACAT CTCAGGCCAC TGAGAGCCAA ACACATAGGT CCTCTATCAA GACATCCCAG 
CCAACGGAAC CCACAGCCTC TGACCTCAAA CCTTCCTCCC CCACAGACCA GCTTGTCACT 
CCCAAGGTCA TAGCTCAGGG TGGTCCAAGC AGGACAGGAA GGGCTTCCAC AGCAAGTGCT 
GTGCTGGTTC CTACTACCCC TGAATTCCAG TCTCCAGTCC CGTCAGAACA GCCTCTTCCC 
CCTGACCCCA TCCCTGAAGT CAACTGCAGC AGGAGGCTGA GGGCCACTAG GAAACATGGG 
TCTCCCACAG CTCATGTTCA TGAGCCCTGC ACCGTACCCC CTGAACCTAA CTCCCACTCT 
TCAAGGAACC AAAGACATGG GGCAGTGAAA GCAGCCAAGC CCCTTAGCAC CATTCCTGAG 
CCTGCCTTTG CCCAGCTCCC CGAGGCACCG CCTCACACTC CCCAGGTGCC AAAGGAGGAG 
GCAGCAGATG GATCAGGCTT CACCCCAGAG CCCCAGCCTA GGGCCTCTCA AAACCACAAG 
AGGCCTTCAG CTACTGCACA TTCACCTCCA CTTCAAAAAC GGCTCCAGAG AGGGAGAGTC 
CCTCAGAAGG CAGCATCCCT TAAGGAAGAA GAAAATCCAG TAGCGAGGCC GAGGAAGGAA 
GAGGGTGTAG TGATTCCAGG TCCAGGCAAG AGAAAGAGAG AGCAGACAGA GGAGGAGTCC 
CAGGGAAGAC CGAGCCGCAG CCTGCGACGG ACCAGACCGA TGCAGGAGTC CACGGCCCCC 
AAAGTGCTGT TCACCGGTGT GGTGGATGCT CGCGGAGAGA GGACGGTGCT GGCCCTGGGG 
GGCAGTTTGG CCAGCTCGGT GGCCGAGGCT TCTCACCTGG TGACTGATCG GATCCGCCGG 
ACGGTCAAGT TTCTGTGTGC CCTGGGCCGG GGCATCCCCA TCCTCTCCCT GGCCTGGCTG 
CATGAGTCCC GCAAGGCAGG CTGCTTCTTG CCCCCGGACG AATATTTGGT GACTGATCCT 
GAGCAGGAGA AGAACTTCGG CTTCAGCCTT CGGGAGGCCC TGAGCCGAGC TCAGGAGCGA 
AGGCTGCTGG AGGGCTATGA GATTCATGTG ACCCCCGGAG TCCAGCCACC GCCACCTCAG 
ATGGGAGAAA TCATCAGCTG CTGTGGAGGC GCCATCCTGC CCAGCATGCC TCGGTCCTAC 
AAGCCTCAGA GGTTCGTGAT CACATGTTCC CAGGACTTTC CTCGATGTGC TGTTCCATAT 
CGGGTTGGGC TGCCTATCCT CTCACCCGAA TTCCTGCTGA CGGGAGTACT GAAGCAGGAA 
GTCAAGCCAG AGGCCTTTGC CTTCTCCACT GTGGAAATGT CATCCACCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MDC1 ( XM_012164403.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_012019793.1 Ovis aries musimon mediator of DNA damage checkpoint 1 (MDC1), transcript variant X12, mRNA.

Price & Availability↑

CloneID	OOv35149
Clone ID Related Accession (Same CDS sequence)	XM_012164403.2
Accession Version	XM_012164403.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5808bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	mediator of DNA damage checkpoint protein 1 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011943095.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012164403.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGTGT CCCACTTTCC TTGTCTACAT TTTCCCTTTA AGATCATGGA AGACACCCAG 
ATTTTAAACT GGGAAGTTGA AGAAGAGGAG GAGGTTGCAG AGAGCCCCAA TGAATCTCTG 
GGGTATAGCT TGGAGCCCCT AGCACAGCTG CATATCTTCA GTAGTTCCTA TGGACCAGAA 
AAAGACTTCC CACTCTACCT TGGAAAGAAC ATGATAGGCC GAATGCCTGA TTGCTCTGTG 
GCCCTGCCCT TTTCATCAAT ATCCAAACAA CATGCAGTGA TTGAAATCTC TGCCTGGGAC 
AAGGCGCCTG TCCTTCGGGA TTGCGGGAGC CTCAATGGCA CTCAAATCCT GCGGCCTCCT 
AAGGTCCTGA GCCCTGGGGT GAGTCATCGT TTGCGGGACC GGGAGTTGAT TCTCTTTGCT 
GACTTGCCCT GCCAGTACCA TCGCTTGGAT GTCCCCCGGC CTTTTGTCTC CCGGGGCCCT 
CTAACTGTAG AGGAGACACC CAGGGTACAG GGAGGAACTC AACCCTCCAG GCTTCTGTTG 
GCTGAGGACT CAGAGGAAGA AGTAGATTCC CTTTTGGACA AGTGTGTGGT GAAAGGACCA 
AGGACCTCCT CTTCGGCAAC AGTCGTTCCA GAGAGTGATG AAGAAGGGCC TTCCCCTGCC 
CCAGATGGGC CCGGGCCACC TTCTGCCTTC AACTTGAACA GCGACACGGA TGAGGAAGAA 
AGTCAGGAAT CAGGAGCAGG GGAGGCCTCT TCAGCTGCCA GAAGAGGTAC CGCTGCAGAG 
ACGGAACAGC CTGAACCTGT CACAGCCGAG ATCCAGATTG AGAAGGATCA GTGTTCCGTG 
AAGGAGAGGA ACGGGGACAC AGAAATCGAG AGGGATGTGA GGAATGGGGT GGTTCCGACT 
GGAGTGATTC TGGAGAGGAG CCAGCCTTCT GGGGAGGACA GTGACACAGA TGTGAGTGAT 
GAGAGTGGGC CTCGAAGAAG GCTTGTTGGA GTCCGTCCGA AAAGGGCCTG GTCTTGTAAC 
TTCATAGACA GTGATACCGA TGGAGAGGAC GAGGGGATCC CTGCTACCCC AGCAGTGGTT 
CCCATGAAGG AGAGGCAGAT CTTCCACGAA GCTGGTACAC AGAGCCCCCA GGCACCTGGT 
GTGGCACGTT GGCAGGAGAG CCCAGCTGAT GGTGATACAG ATATAGAGGA GGGGGAGGCC 
CCCCCAGACA GAAGCCAAGC CTCCGTGGTG ATCGACAGCA ATACAGACGA TGAGGAAGAA 
GTCTCAGCAG CACTGACACT GGCATGTCTA AGAGAGAGCC AGGCTGGTAA GTGGAACCGA 
GATCCAGATG CAGAAGAGAC CAGGGCCCAA CCCGTGGCCC TTCTGGAGCG AAGCCAGGCC 
TCTGCTGGGG GAGACAGTGA CACAGATGTG GAGGAAGAGG GGCTCCCAGT GGAAAGGAGG 
GGAATGGTTC CCAAGGGTTA CATGGACAGG GAATATTCAA AAAAGAGCCA GCACCCTCCC 
AGGGACAGTG ATACAGAGGG GAAGGAAGAT GAGAGCTCAC CGGGGGTCCA CCTGGAGAGA 
AGCCAGGCCT CTGCACAAGT GGAGGATGAG GTCCCACTGG GGCCAGCTGT TGCACTTCCG 
GAGAAGCGTC AGGTACAAGG CATAGTGTGG CCACATCACA CCGATGCGGA GGCAGAAGGG 
GACCCGGCAC AGCTCCCCGT GCTGCGTCTA GAGGAAGCCC GGCCTCCTCT AGCTGGGGAC 
TGTGAACTGG ATTCGGAGAA CACATCTGCT GTCAGAAAGA GCCAGCTTCC GGCGGAAAAA 
GATGCTGGGA CCACGTGGGC TGCAGCCGTT CCTGAACAGG ACAGAGCACT TGCCATCGGG 
ACCCAGGGTG GGTCATCCAC GGCACCAGGG GAGCAAGACC TTCTCCCGGT CTCAAGGGAA 
GACCTAGCAG ATATGGTGGT GGACATAGGC ACTCCAGGGG AGCCCCAACC GCAGAGAGAG 
GGGGCCCAGA CCACCACAGG AAGGGAGAGA GAACCATATG GGAATAGGGC CACAGACTCT 
GGAGACAACC TCCACGATTC TGAAGATCTG GACCTACCAG CTACCCAGTG CTTTGTAGAC 
AGAAAGAATC AGAGCCTGGA AGCCCCCAGC ATGGAGGATG AGCCCACCCA GGCCTTCCTG 
TTTCCTCTGC CCCAAGAGCC TGGCCCTTCC TGTTGCAGCT CCCAGGCCAC AGGTTCCTTG 
GATGAGTCAT GGGAGGCCTT GGCGACACAG CCATTCTGTC CGAGAGAATC TGAGGCCTCC 
GAGACCCAAA CCGTTGTCAC CCTCCTTGAC ACCCATGCAT CTTGGCCCTC TCCATCTAGG 
ACAGCACAGC AAGAGCAACA TCCAGAGAGC CCAGTCCATG CAGAGCCACT GGGGATGGAA 
GGCAGAGAGA TGCAGACTGT GGAGAAAGAA ATGGGTACCC CAAGAGAAAC AGCAGAGAGG 
GTGATCCCTG AGGGAGGGCC ACCGCAGGAG GAAACCAAGA AACTGCCCTC AGAAGGAGAG 
AGGGAAGATG TGACGGGAGA GGAAGAATTA ATCGGGGGGA TACAGGGCAG AGAAAAAAAT 
CAGGTGTTTG CTAGAGATAC TCAGAGCCAA GAATCTGACA AAAAAGTGAA AAGTGCAAGT 
ACTGGAAGGG GAATGGAGAT TTTAAAGGTA GAAATTGAGA CACCCAAGGA AACACAAGAG 
AAAGAGAGAG AAAAGCAGAC TCTCGCAGGG GAAATATTTG AGAGTGAAGC AGGGAAACTG 
GTAGTAGAGA GAGAGAGTGA GGCAGATGGG TTAGAAGTCA AGGAACCCCA AGAGCTACTG 
GACAGAAGCC CACAGATGGG GGAGACAGAG GCGGGGGACC AGGACCAGAA AGGCCAAGCC 
TCTGGTCTGC CATCAGAGCC TGGAGCAGGG GCAGGAGACC TTCAGGGACT TGCTTCAGAC 
CCAGTAGCTT CTGGGAGCCA GGCAGGTGGA GGAAGGGGAG TCCCAGGGAG CCCCAGGAGG 
CAGCAGAGAG GTGACTTGAA TTGCGAGATG CCACCTGCTC AGAAGGCTTC CAGGGGTGAT 
CAGGAATCCC CAGATGCTTG TCTGCCTCCT GCAGCGCCTG AAGCCTCAGC CGCACTCCCA 
AATTCCCTCA TCATCTCTCA GATCCAAAAA CATCCCGCAC CTCAGTCCCT CCTTTTTCCC 
TCTCTAGCTC CTTTAGAACT GCCTATTCCC AGGACCAGAC AAAATGAGAC TCAGGAAGCT 
CCAGAGACTC CCTTCTCCTC AGAGCTGAAC TCTGTCCACC CAGAACCCAA AGTCAGGCTC 
CAGGCGTCCT CTCCAGTTTC TTCTCTACCC CTTGAGCCCC ACCCTACCAC CCCCACAGGC 
CAGCCTATTG CCCTTGAACC CATATCTGGG GTCTCTTGGA GCAGGACACG TAGTTCCTTT 
GATGTAACTG CCTCATCAGT TGTCCCCACA GCCGTTGCAC TGCAGCCATC CATCTCCACA 
GACAAGCCTG TCACCCCTAA GCCCACACTT CGGGCCCCTC GGGGCAGGGC ACAGAGGTCT 
TCTGTCAAAA CCCCTGAACC CAATGTCACC ACAGACCAGC CTGTTGCCCC TGAGCTCACA 
GCTATGGCAA CTCGGGGCAG GGCACAGAGG TCTTCTGTCA AGATTCCCAA ACCAGATAAC 
CCCACAACAC CCAAGCCCCA GCCTTCCACT TCCACAGACC AGCCTGTCAT CCCCAAACCC 
ACATCTCGGG CCCCTCGGGG CAGGACACCT AGGTCTTCTG CCAAGACTCC TGAACCAGCT 
GTCCCCACAG CCTCTGAGCT CCAGCCTGCT GCCCCTAAAG ACCAGCCTGT TGCTCCTGAG 
CTCACATCTA GAGCCACTCG GGGCAGGACA CAGAGGTCTA TCAAGACTTC CAAACCAGAT 
ATGCCCACAA CTCCCGAGCC CCAGCCTTCC ACCTCCGCAG ACCAGCCTGT CACCCCCAAA 
CCCACGTCTC GGGCCCCTCG GGGCAGGACA CCTAGGTCTT CCGCCAAGAC TCCTGAGCCA 
GTTGTCTCCA CAGCCTCTGA GCTCCAGCCT TCTGCCCTCA CAGATGAGCC TGTCACTCCT 
GAGCTCACAT CTAGGGCTAC TCGGGGTCGG GCACAGAGGT CCTCTGTCAA AACCCCTGAT 
CCAGTTACCA CCACAACACC TGAGCTCCAG CCTTCCACCT CCACAGACCA GCTTGTCACC 
CCCAAACGCA CATCTCGGGC CCCTCGAGGC AGGACACGTA GGTTGTCTGC CAAGACTCCT 
GAACCAGTTG TTCCCACAGC CTCTGAGCTC CAGCCTTCTG CCCCTGCAGA CCAGCCTGTT 
GGTCCTTGGG CCACTCAGTG TAGAAGACAT AGGTCTTCTG TCAAGACCCC AGAACCAGTT 
GTCCCCACAG TCCCTGAACC TCAGCCTTCC ACTTCTAAAG ACCAGTCTGT CACTCCTGAG 
CCCACATCTC AGGCCACTGA GAGCCAAACA CATAGGTCCT CTATCAAGAC ATCCCAGCCA 
ACGGAACCCA CAGCCTCTGA CCTCAAACCT TCCTCCCCCA CAGACCAGCT TGTCACTCCC 
AAGGTCATAG CTCAGGGTGG TCCAAGCAGG ACAGGAAGGG CTTCCACAGC AAGTGCTGTG 
CTGGTTCCTA CTACCCCTGA ATTCCAGTCT CCAGTCCCGT CAGAACAGCC TCTTCCCCCT 
GACCCCATCC CTGAAGTCAA CTGCAGCAGG AGGCTGAGGG CCACTAGGAA ACATGGGTCT 
CCCACAGCTC ATGTTCATGA GCCCTGCACC GTACCCCCTG AACCTAACTC CCACTCTTCA 
AGGAACCAAA GACATGGGGC AGTGAAAGCA GCCAAGCCCC TTAGCACCAT TCCTGAGCCT 
GCCTTTGCCC AGCTCCCCGA GGCACCGCCT CACACTCCCC AGGTGCCAAA GGAGGAGGCA 
GCAGATGGAT CAGGCTTCAC CCCAGAGCCC CAGCCTAGGG CCTCTCAAAA CCACAAGAGG 
CCTTCAGCTA CTGCACATTC ACCTCCACTT CAAAAACGGC TCCAGAGAGG GAGAGTCCCT 
CAGAAGGCAG CATCCCTTAA GGAAGAAGAA AATCCAGTAG CGAGGCCGAG GAAGGAAGAG 
GGTGTAGTGA TTCCAGGTCC AGGCAAGAGA AAGAGAGAGC AGACAGAGGA GGAGTCCCAG 
GGAAGACCGA GCCGCAGCCT GCGACGGACC AGACCGATGC AGGAGTCCAC GGCCCCCAAA 
GTGCTGTTCA CCGGTGTGGT GGATGCTCGC GGAGAGAGGA CGGTGCTGGC CCTGGGGGGC 
AGTTTGGCCA GCTCGGTGGC CGAGGCTTCT CACCTGGTGA CTGATCGGAT CCGCCGGACG 
GTCAAGTTTC TGTGTGCCCT GGGCCGGGGC ATCCCCATCC TCTCCCTGGC CTGGCTGCAT 
GAGTCCCGCA AGGCAGGCTG CTTCTTGCCC CCGGACGAAT ATTTGGTGAC TGATCCTGAG 
CAGGAGAAGA ACTTCGGCTT CAGCCTTCGG GAGGCCCTGA GCCGAGCTCA GGAGCGAAGG 
CTGCTGGAGG GCTATGAGAT TCATGTGACC CCCGGAGTCC AGCCACCGCC ACCTCAGATG 
GGAGAAATCA TCAGCTGCTG TGGAGGCGCC ATCCTGCCCA GCATGCCTCG GTCCTACAAG 
CCTCAGAGGT TCGTGATCAC ATGTTCCCAG GACTTTCCTC GATGTGCTGT TCCATATCGG 
GTTGGGCTGC CTATCCTCTC ACCCGAATTC CTGCTGACGG GAGTACTGAA GCAGGAAGTC 
AAGCCAGAGG CCTTTGCCTT CTCCACTGTG GAAATGTCAT CCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012019793.1
CDS	328..6135
Translation	MEVSHFPCLHFPFKIMEDTQILNWEVEEEEEVAESPNESLGYSLEPLAQLHIFSSSYGPEKDFPLYLGKNMIGRMPDCSVALPFSSISKQHAVIEISAWDKAPVLRDCGSLNGTQILRPPKVLSPGVSHRLRDRELILFADLPCQYHRLDVPRPFVSRGPLTVEETPRVQGGTQPSRLLLAEDSEEEVDSLLDKCVVKGPRTSSSATVVPESDEEGPSPAPDGPGPPSAFNLNSDTDEEESQESGAGEASSAARRGTAAETEQPEPVTAEIQIEKDQCSVKERNGDTEIERDVRNGVVPTGVILERSQPSGEDSDTDVSDESGPRRRLVGVRPKRAWSCNFIDSDTDGEDEGIPATPAVVPMKERQIFHEAGTQSPQAPGVARWQESPADGDTDIEEGEAPPDRSQASVVIDSNTDDEEEVSAALTLACLRESQAGKWNRDPDAEETRAQPVALLERSQASAGGDSDTDVEEEGLPVERRGMVPKGYMDREYSKKSQHPPRDSDTEGKEDESSPGVHLERSQASAQVEDEVPLGPAVALPEKRQVQGIVWPHHTDAEAEGDPAQLPVLRLEEARPPLAGDCELDSENTSAVRKSQLPAEKDAGTTWAAAVPEQDRALAIGTQGGSSTAPGEQDLLPVSREDLADMVVDIGTPGEPQPQREGAQTTTGREREPYGNRATDSGDNLHDSEDLDLPATQCFVDRKNQSLEAPSMEDEPTQAFLFPLPQEPGPSCCSSQATGSLDESWEALATQPFCPRESEASETQTVVTLLDTHASWPSPSRTAQQEQHPESPVHAEPLGMEGREMQTVEKEMGTPRETAERVIPEGGPPQEETKKLPSEGEREDVTGEEELIGGIQGREKNQVFARDTQSQESDKKVKSASTGRGMEILKVEIETPKETQEKEREKQTLAGEIFESEAGKLVVERESEADGLEVKEPQELLDRSPQMGETEAGDQDQKGQASGLPSEPGAGAGDLQGLASDPVASGSQAGGGRGVPGSPRRQQRGDLNCEMPPAQKASRGDQESPDACLPPAAPEASAALPNSLIISQIQKHPAPQSLLFPSLAPLELPIPRTRQNETQEAPETPFSSELNSVHPEPKVRLQASSPVSSLPLEPHPTTPTGQPIALEPISGVSWSRTRSSFDVTASSVVPTAVALQPSISTDKPVTPKPTLRAPRGRAQRSSVKTPEPNVTTDQPVAPELTAMATRGRAQRSSVKIPKPDNPTTPKPQPSTSTDQPVIPKPTSRAPRGRTPRSSAKTPEPAVPTASELQPAAPKDQPVAPELTSRATRGRTQRSIKTSKPDMPTTPEPQPSTSADQPVTPKPTSRAPRGRTPRSSAKTPEPVVSTASELQPSALTDEPVTPELTSRATRGRAQRSSVKTPDPVTTTTPELQPSTSTDQLVTPKRTSRAPRGRTRRLSAKTPEPVVPTASELQPSAPADQPVGPWATQCRRHRSSVKTPEPVVPTVPEPQPSTSKDQSVTPEPTSQATESQTHRSSIKTSQPTEPTASDLKPSSPTDQLVTPKVIAQGGPSRTGRASTASAVLVPTTPEFQSPVPSEQPLPPDPIPEVNCSRRLRATRKHGSPTAHVHEPCTVPPEPNSHSSRNQRHGAVKAAKPLSTIPEPAFAQLPEAPPHTPQVPKEEAADGSGFTPEPQPRASQNHKRPSATAHSPPLQKRLQRGRVPQKAASLKEEENPVARPRKEEGVVIPGPGKRKREQTEEESQGRPSRSLRRTRPMQESTAPKVLFTGVVDARGERTVLALGGSLASSVAEASHLVTDRIRRTVKFLCALGRGIPILSLAWLHESRKAGCFLPPDEYLVTDPEQEKNFGFSLREALSRAQERRLLEGYEIHVTPGVQPPPPQMGEIISCCGGAILPSMPRSYKPQRFVITCSQDFPRCAVPYRVGLPILSPEFLLTGVLKQEVKPEAFAFSTVEMSST

Target ORF information:

RefSeq Version	XM_012164403.2
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon mediator of DNA damage checkpoint 1 (MDC1), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012164403.2

ORF Insert Sequence:

ATGGAGGTGT CCCACTTTCC TTGTCTACAT TTTCCCTTTA AGATCATGGA AGACACCCAG 
ATTTTAAACT GGGAAGTTGA AGAAGAGGAG GAGGTTGCAG AGAGCCCCAA TGAATCTCTG 
GGGTATAGCT TGGAGCCCCT AGCACAGCTG CATATCTTCA GTAGTTCCTA TGGACCAGAA 
AAAGACTTCC CACTCTACCT TGGAAAGAAC ATGATAGGCC GAATGCCTGA TTGCTCTGTG 
GCCCTGCCCT TTTCATCAAT ATCCAAACAA CATGCAGTGA TTGAAATCTC TGCCTGGGAC 
AAGGCGCCTG TCCTTCGGGA TTGCGGGAGC CTCAATGGCA CTCAAATCCT GCGGCCTCCT 
AAGGTCCTGA GCCCTGGGGT GAGTCATCGT TTGCGGGACC GGGAGTTGAT TCTCTTTGCT 
GACTTGCCCT GCCAGTACCA TCGCTTGGAT GTCCCCCGGC CTTTTGTCTC CCGGGGCCCT 
CTAACTGTAG AGGAGACACC CAGGGTACAG GGAGGAACTC AACCCTCCAG GCTTCTGTTG 
GCTGAGGACT CAGAGGAAGA AGTAGATTCC CTTTTGGACA AGTGTGTGGT GAAAGGACCA 
AGGACCTCCT CTTCGGCAAC AGTCGTTCCA GAGAGTGATG AAGAAGGGCC TTCCCCTGCC 
CCAGATGGGC CCGGGCCACC TTCTGCCTTC AACTTGAACA GCGACACGGA TGAGGAAGAA 
AGTCAGGAAT CAGGAGCAGG GGAGGCCTCT TCAGCTGCCA GAAGAGGTAC CGCTGCAGAG 
ACGGAACAGC CTGAACCTGT CACAGCCGAG ATCCAGATTG AGAAGGATCA GTGTTCCGTG 
AAGGAGAGGA ACGGGGACAC AGAAATCGAG AGGGATGTGA GGAATGGGGT GGTTCCGACT 
GGAGTGATTC TGGAGAGGAG CCAGCCTTCT GGGGAGGACA GTGACACAGA TGTGAGTGAT 
GAGAGTGGGC CTCGAAGAAG GCTTGTTGGA GTCCGTCCGA AAAGGGCCTG GTCTTGTAAC 
TTCATAGACA GTGATACCGA TGGAGAGGAC GAGGGGATCC CTGCTACCCC AGCAGTGGTT 
CCCATGAAGG AGAGGCAGAT CTTCCACGAA GCTGGTACAC AGAGCCCCCA GGCACCTGGT 
GTGGCACGTT GGCAGGAGAG CCCAGCTGAT GGTGATACAG ATATAGAGGA GGGGGAGGCC 
CCCCCAGACA GAAGCCAAGC CTCCGTGGTG ATCGACAGCA ATACAGACGA TGAGGAAGAA 
GTCTCAGCAG CACTGACACT GGCATGTCTA AGAGAGAGCC AGGCTGGTAA GTGGAACCGA 
GATCCAGATG CAGAAGAGAC CAGGGCCCAA CCCGTGGCCC TTCTGGAGCG AAGCCAGGCC 
TCTGCTGGGG GAGACAGTGA CACAGATGTG GAGGAAGAGG GGCTCCCAGT GGAAAGGAGG 
GGAATGGTTC CCAAGGGTTA CATGGACAGG GAATATTCAA AAAAGAGCCA GCACCCTCCC 
AGGGACAGTG ATACAGAGGG GAAGGAAGAT GAGAGCTCAC CGGGGGTCCA CCTGGAGAGA 
AGCCAGGCCT CTGCACAAGT GGAGGATGAG GTCCCACTGG GGCCAGCTGT TGCACTTCCG 
GAGAAGCGTC AGGTACAAGG CATAGTGTGG CCACATCACA CCGATGCGGA GGCAGAAGGG 
GACCCGGCAC AGCTCCCCGT GCTGCGTCTA GAGGAAGCCC GGCCTCCTCT AGCTGGGGAC 
TGTGAACTGG ATTCGGAGAA CACATCTGCT GTCAGAAAGA GCCAGCTTCC GGCGGAAAAA 
GATGCTGGGA CCACGTGGGC TGCAGCCGTT CCTGAACAGG ACAGAGCACT TGCCATCGGG 
ACCCAGGGTG GGTCATCCAC GGCACCAGGG GAGCAAGACC TTCTCCCGGT CTCAAGGGAA 
GACCTAGCAG ATATGGTGGT GGACATAGGC ACTCCAGGGG AGCCCCAACC GCAGAGAGAG 
GGGGCCCAGA CCACCACAGG AAGGGAGAGA GAACCATATG GGAATAGGGC CACAGACTCT 
GGAGACAACC TCCACGATTC TGAAGATCTG GACCTACCAG CTACCCAGTG CTTTGTAGAC 
AGAAAGAATC AGAGCCTGGA AGCCCCCAGC ATGGAGGATG AGCCCACCCA GGCCTTCCTG 
TTTCCTCTGC CCCAAGAGCC TGGCCCTTCC TGTTGCAGCT CCCAGGCCAC AGGTTCCTTG 
GATGAGTCAT GGGAGGCCTT GGCGACACAG CCATTCTGTC CGAGAGAATC TGAGGCCTCC 
GAGACCCAAA CCGTTGTCAC CCTCCTTGAC ACCCATGCAT CTTGGCCCTC TCCATCTAGG 
ACAGCACAGC AAGAGCAACA TCCAGAGAGC CCAGTCCATG CAGAGCCACT GGGGATGGAA 
GGCAGAGAGA TGCAGACTGT GGAGAAAGAA ATGGGTACCC CAAGAGAAAC AGCAGAGAGG 
GTGATCCCTG AGGGAGGGCC ACCGCAGGAG GAAACCAAGA AACTGCCCTC AGAAGGAGAG 
AGGGAAGATG TGACGGGAGA GGAAGAATTA ATCGGGGGGA TACAGGGCAG AGAAAAAAAT 
CAGGTGTTTG CTAGAGATAC TCAGAGCCAA GAATCTGACA AAAAAGTGAA AAGTGCAAGT 
ACTGGAAGGG GAATGGAGAT TTTAAAGGTA GAAATTGAGA CACCCAAGGA AACACAAGAG 
AAAGAGAGAG AAAAGCAGAC TCTCGCAGGG GAAATATTTG AGAGTGAAGC AGGGAAACTG 
GTAGTAGAGA GAGAGAGTGA GGCAGATGGG TTAGAAGTCA AGGAACCCCA AGAGCTACTG 
GACAGAAGCC CACAGATGGG GGAGACAGAG GCGGGGGACC AGGACCAGAA AGGCCAAGCC 
TCTGGTCTGC CATCAGAGCC TGGAGCAGGG GCAGGAGACC TTCAGGGACT TGCTTCAGAC 
CCAGTAGCTT CTGGGAGCCA GGCAGGTGGA GGAAGGGGAG TCCCAGGGAG CCCCAGGAGG 
CAGCAGAGAG GTGACTTGAA TTGCGAGATG CCACCTGCTC AGAAGGCTTC CAGGGGTGAT 
CAGGAATCCC CAGATGCTTG TCTGCCTCCT GCAGCGCCTG AAGCCTCAGC CGCACTCCCA 
AATTCCCTCA TCATCTCTCA GATCCAAAAA CATCCCGCAC CTCAGTCCCT CCTTTTTCCC 
TCTCTAGCTC CTTTAGAACT GCCTATTCCC AGGACCAGAC AAAATGAGAC TCAGGAAGCT 
CCAGAGACTC CCTTCTCCTC AGAGCTGAAC TCTGTCCACC CAGAACCCAA AGTCAGGCTC 
CAGGCGTCCT CTCCAGTTTC TTCTCTACCC CTTGAGCCCC ACCCTACCAC CCCCACAGGC 
CAGCCTATTG CCCTTGAACC CATATCTGGG GTCTCTTGGA GCAGGACACG TAGTTCCTTT 
GATGTAACTG CCTCATCAGT TGTCCCCACA GCCGTTGCAC TGCAGCCATC CATCTCCACA 
GACAAGCCTG TCACCCCTAA GCCCACACTT CGGGCCCCTC GGGGCAGGGC ACAGAGGTCT 
TCTGTCAAAA CCCCTGAACC CAATGTCACC ACAGACCAGC CTGTTGCCCC TGAGCTCACA 
GCTATGGCAA CTCGGGGCAG GGCACAGAGG TCTTCTGTCA AGATTCCCAA ACCAGATAAC 
CCCACAACAC CCAAGCCCCA GCCTTCCACT TCCACAGACC AGCCTGTCAT CCCCAAACCC 
ACATCTCGGG CCCCTCGGGG CAGGACACCT AGGTCTTCTG CCAAGACTCC TGAACCAGCT 
GTCCCCACAG CCTCTGAGCT CCAGCCTGCT GCCCCTAAAG ACCAGCCTGT TGCTCCTGAG 
CTCACATCTA GAGCCACTCG GGGCAGGACA CAGAGGTCTA TCAAGACTTC CAAACCAGAT 
ATGCCCACAA CTCCCGAGCC CCAGCCTTCC ACCTCCGCAG ACCAGCCTGT CACCCCCAAA 
CCCACGTCTC GGGCCCCTCG GGGCAGGACA CCTAGGTCTT CCGCCAAGAC TCCTGAGCCA 
GTTGTCTCCA CAGCCTCTGA GCTCCAGCCT TCTGCCCTCA CAGATGAGCC TGTCACTCCT 
GAGCTCACAT CTAGGGCTAC TCGGGGTCGG GCACAGAGGT CCTCTGTCAA AACCCCTGAT 
CCAGTTACCA CCACAACACC TGAGCTCCAG CCTTCCACCT CCACAGACCA GCTTGTCACC 
CCCAAACGCA CATCTCGGGC CCCTCGAGGC AGGACACGTA GGTTGTCTGC CAAGACTCCT 
GAACCAGTTG TTCCCACAGC CTCTGAGCTC CAGCCTTCTG CCCCTGCAGA CCAGCCTGTT 
GGTCCTTGGG CCACTCAGTG TAGAAGACAT AGGTCTTCTG TCAAGACCCC AGAACCAGTT 
GTCCCCACAG TCCCTGAACC TCAGCCTTCC ACTTCTAAAG ACCAGTCTGT CACTCCTGAG 
CCCACATCTC AGGCCACTGA GAGCCAAACA CATAGGTCCT CTATCAAGAC ATCCCAGCCA 
ACGGAACCCA CAGCCTCTGA CCTCAAACCT TCCTCCCCCA CAGACCAGCT TGTCACTCCC 
AAGGTCATAG CTCAGGGTGG TCCAAGCAGG ACAGGAAGGG CTTCCACAGC AAGTGCTGTG 
CTGGTTCCTA CTACCCCTGA ATTCCAGTCT CCAGTCCCGT CAGAACAGCC TCTTCCCCCT 
GACCCCATCC CTGAAGTCAA CTGCAGCAGG AGGCTGAGGG CCACTAGGAA ACATGGGTCT 
CCCACAGCTC ATGTTCATGA GCCCTGCACC GTACCCCCTG AACCTAACTC CCACTCTTCA 
AGGAACCAAA GACATGGGGC AGTGAAAGCA GCCAAGCCCC TTAGCACCAT TCCTGAGCCT 
GCCTTTGCCC AGCTCCCCGA GGCACCGCCT CACACTCCCC AGGTGCCAAA GGAGGAGGCA 
GCAGATGGAT CAGGCTTCAC CCCAGAGCCC CAGCCTAGGG CCTCTCAAAA CCACAAGAGG 
CCTTCAGCTA CTGCACATTC ACCTCCACTT CAAAAACGGC TCCAGAGAGG GAGAGTCCCT 
CAGAAGGCAG CATCCCTTAA GGAAGAAGAA AATCCAGTAG CGAGGCCGAG GAAGGAAGAG 
GGTGTAGTGA TTCCAGGTCC AGGCAAGAGA AAGAGAGAGC AGACAGAGGA GGAGTCCCAG 
GGAAGACCGA GCCGCAGCCT GCGACGGACC AGACCGATGC AGGAGTCCAC GGCCCCCAAA 
GTGCTGTTCA CCGGTGTGGT GGATGCTCGC GGAGAGAGGA CGGTGCTGGC CCTGGGGGGC 
AGTTTGGCCA GCTCGGTGGC CGAGGCTTCT CACCTGGTGA CTGATCGGAT CCGCCGGACG 
GTCAAGTTTC TGTGTGCCCT GGGCCGGGGC ATCCCCATCC TCTCCCTGGC CTGGCTGCAT 
GAGTCCCGCA AGGCAGGCTG CTTCTTGCCC CCGGACGAAT ATTTGGTGAC TGATCCTGAG 
CAGGAGAAGA ACTTCGGCTT CAGCCTTCGG GAGGCCCTGA GCCGAGCTCA GGAGCGAAGG 
CTGCTGGAGG GCTATGAGAT TCATGTGACC CCCGGAGTCC AGCCACCGCC ACCTCAGATG 
GGAGAAATCA TCAGCTGCTG TGGAGGCGCC ATCCTGCCCA GCATGCCTCG GTCCTACAAG 
CCTCAGAGGT TCGTGATCAC ATGTTCCCAG GACTTTCCTC GATGTGCTGT TCCATATCGG 
GTTGGGCTGC CTATCCTCTC ACCCGAATTC CTGCTGACGG GAGTACTGAA GCAGGAAGTC 
AAGCCAGAGG CCTTTGCCTT CTCCACTGTG GAAATGTCAT CCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MDC1 ( XM_012164405.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_012019795.1 Ovis aries musimon mediator of DNA damage checkpoint 1 (MDC1), transcript variant X14, mRNA.

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CloneID	OOv35150
Clone ID Related Accession (Same CDS sequence)	XM_012164405.2 , XM_012164407.2 , XM_012164406.2 , XM_012164404.2
Accession Version	XM_012164405.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5766bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	mediator of DNA damage checkpoint protein 1 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011943095.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012164405.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGACA CCCAGATTTT AAACTGGGAA GTTGAAGAAG AGGAGGAGGT TGCAGAGAGC 
CCCAATGAAT CTCTGGGGTA TAGCTTGGAG CCCCTAGCAC AGCTGCATAT CTTCAGTAGT 
TCCTATGGAC CAGAAAAAGA CTTCCCACTC TACCTTGGAA AGAACATGAT AGGCCGAATG 
CCTGATTGCT CTGTGGCCCT GCCCTTTTCA TCAATATCCA AACAACATGC AGTGATTGAA 
ATCTCTGCCT GGGACAAGGC GCCTGTCCTT CGGGATTGCG GGAGCCTCAA TGGCACTCAA 
ATCCTGCGGC CTCCTAAGGT CCTGAGCCCT GGGGTGAGTC ATCGTTTGCG GGACCGGGAG 
TTGATTCTCT TTGCTGACTT GCCCTGCCAG TACCATCGCT TGGATGTCCC CCGGCCTTTT 
GTCTCCCGGG GCCCTCTAAC TGTAGAGGAG ACACCCAGGG TACAGGGAGG AACTCAACCC 
TCCAGGCTTC TGTTGGCTGA GGACTCAGAG GAAGAAGTAG ATTCCCTTTT GGACAAGTGT 
GTGGTGAAAG GACCAAGGAC CTCCTCTTCG GCAACAGTCG TTCCAGAGAG TGATGAAGAA 
GGGCCTTCCC CTGCCCCAGA TGGGCCCGGG CCACCTTCTG CCTTCAACTT GAACAGCGAC 
ACGGATGAGG AAGAAAGTCA GGAATCAGGA GCAGGGGAGG CCTCTTCAGC TGCCAGAAGA 
GGTACCGCTG CAGAGACGGA ACAGCCTGAA CCTGTCACAG CCGAGATCCA GATTGAGAAG 
GATCAGTGTT CCGTGAAGGA GAGGAACGGG GACACAGAAA TCGAGAGGGA TGTGAGGAAT 
GGGGTGGTTC CGACTGGAGT GATTCTGGAG AGGAGCCAGC CTTCTGGGGA GGACAGTGAC 
ACAGATGTGA GTGATGAGAG TGGGCCTCGA AGAAGGCTTG TTGGAGTCCG TCCGAAAAGG 
GCCTGGTCTT GTAACTTCAT AGACAGTGAT ACCGATGGAG AGGACGAGGG GATCCCTGCT 
ACCCCAGCAG TGGTTCCCAT GAAGGAGAGG CAGATCTTCC ACGAAGCTGG TACACAGAGC 
CCCCAGGCAC CTGGTGTGGC ACGTTGGCAG GAGAGCCCAG CTGATGGTGA TACAGATATA 
GAGGAGGGGG AGGCCCCCCC AGACAGAAGC CAAGCCTCCG TGGTGATCGA CAGCAATACA 
GACGATGAGG AAGAAGTCTC AGCAGCACTG ACACTGGCAT GTCTAAGAGA GAGCCAGGCT 
GGTAAGTGGA ACCGAGATCC AGATGCAGAA GAGACCAGGG CCCAACCCGT GGCCCTTCTG 
GAGCGAAGCC AGGCCTCTGC TGGGGGAGAC AGTGACACAG ATGTGGAGGA AGAGGGGCTC 
CCAGTGGAAA GGAGGGGAAT GGTTCCCAAG GGTTACATGG ACAGGGAATA TTCAAAAAAG 
AGCCAGCACC CTCCCAGGGA CAGTGATACA GAGGGGAAGG AAGATGAGAG CTCACCGGGG 
GTCCACCTGG AGAGAAGCCA GGCCTCTGCA CAAGTGGAGG ATGAGGTCCC ACTGGGGCCA 
GCTGTTGCAC TTCCGGAGAA GCGTCAGGTA CAAGGCATAG TGTGGCCACA TCACACCGAT 
GCGGAGGCAG AAGGGGACCC GGCACAGCTC CCCGTGCTGC GTCTAGAGGA AGCCCGGCCT 
CCTCTAGCTG GGGACTGTGA ACTGGATTCG GAGAACACAT CTGCTGTCAG AAAGAGCCAG 
CTTCCGGCGG AAAAAGATGC TGGGACCACG TGGGCTGCAG CCGTTCCTGA ACAGGACAGA 
GCACTTGCCA TCGGGACCCA GGGTGGGTCA TCCACGGCAC CAGGGGAGCA AGACCTTCTC 
CCGGTCTCAA GGGAAGACCT AGCAGATATG GTGGTGGACA TAGGCACTCC AGGGGAGCCC 
CAACCGCAGA GAGAGGGGGC CCAGACCACC ACAGGAAGGG AGAGAGAACC ATATGGGAAT 
AGGGCCACAG ACTCTGGAGA CAACCTCCAC GATTCTGAAG ATCTGGACCT ACCAGCTACC 
CAGTGCTTTG TAGACAGAAA GAATCAGAGC CTGGAAGCAG CCCCCAGCAT GGAGGATGAG 
CCCACCCAGG CCTTCCTGTT TCCTCTGCCC CAAGAGCCTG GCCCTTCCTG TTGCAGCTCC 
CAGGCCACAG GTTCCTTGGA TGAGTCATGG GAGGCCTTGG CGACACAGCC ATTCTGTCCG 
AGAGAATCTG AGGCCTCCGA GACCCAAACC GTTGTCACCC TCCTTGACAC CCATGCATCT 
TGGCCCTCTC CATCTAGGAC AGCACAGCAA GAGCAACATC CAGAGAGCCC AGTCCATGCA 
GAGCCACTGG GGATGGAAGG CAGAGAGATG CAGACTGTGG AGAAAGAAAT GGGTACCCCA 
AGAGAAACAG CAGAGAGGGT GATCCCTGAG GGAGGGCCAC CGCAGGAGGA AACCAAGAAA 
CTGCCCTCAG AAGGAGAGAG GGAAGATGTG ACGGGAGAGG AAGAATTAAT CGGGGGGATA 
CAGGGCAGAG AAAAAAATCA GGTGTTTGCT AGAGATACTC AGAGCCAAGA ATCTGACAAA 
AAAGTGAAAA GTGCAAGTAC TGGAAGGGGA ATGGAGATTT TAAAGGTAGA AATTGAGACA 
CCCAAGGAAA CACAAGAGAA AGAGAGAGAA AAGCAGACTC TCGCAGGGGA AATATTTGAG 
AGTGAAGCAG GGAAACTGGT AGTAGAGAGA GAGAGTGAGG CAGATGGGTT AGAAGTCAAG 
GAACCCCAAG AGCTACTGGA CAGAAGCCCA CAGATGGGGG AGACAGAGGC GGGGGACCAG 
GACCAGAAAG GCCAAGCCTC TGGTCTGCCA TCAGAGCCTG GAGCAGGGGC AGGAGACCTT 
CAGGGACTTG CTTCAGACCC AGTAGCTTCT GGGAGCCAGG CAGGTGGAGG AAGGGGAGTC 
CCAGGGAGCC CCAGGAGGCA GCAGAGAGGT GACTTGAATT GCGAGATGCC ACCTGCTCAG 
AAGGCTTCCA GGGGTGATCA GGAATCCCCA GATGCTTGTC TGCCTCCTGC AGCGCCTGAA 
GCCTCAGCCG CACTCCCAAA TTCCCTCATC ATCTCTCAGA TCCAAAAACA TCCCGCACCT 
CAGTCCCTCC TTTTTCCCTC TCTAGCTCCT TTAGAACTGC CTATTCCCAG GACCAGACAA 
AATGAGACTC AGGAAGCTCC AGAGACTCCC TTCTCCTCAG AGCTGAACTC TGTCCACCCA 
GAACCCAAAG TCAGGCTCCA GGCGTCCTCT CCAGTTTCTT CTCTACCCCT TGAGCCCCAC 
CCTACCACCC CCACAGGCCA GCCTATTGCC CTTGAACCCA TATCTGGGGT CTCTTGGAGC 
AGGACACGTA GTTCCTTTGA TGTAACTGCC TCATCAGTTG TCCCCACAGC CGTTGCACTG 
CAGCCATCCA TCTCCACAGA CAAGCCTGTC ACCCCTAAGC CCACACTTCG GGCCCCTCGG 
GGCAGGGCAC AGAGGTCTTC TGTCAAAACC CCTGAACCCA ATGTCACCAC AGACCAGCCT 
GTTGCCCCTG AGCTCACAGC TATGGCAACT CGGGGCAGGG CACAGAGGTC TTCTGTCAAG 
ATTCCCAAAC CAGATAACCC CACAACACCC AAGCCCCAGC CTTCCACTTC CACAGACCAG 
CCTGTCATCC CCAAACCCAC ATCTCGGGCC CCTCGGGGCA GGACACCTAG GTCTTCTGCC 
AAGACTCCTG AACCAGCTGT CCCCACAGCC TCTGAGCTCC AGCCTGCTGC CCCTAAAGAC 
CAGCCTGTTG CTCCTGAGCT CACATCTAGA GCCACTCGGG GCAGGACACA GAGGTCTATC 
AAGACTTCCA AACCAGATAT GCCCACAACT CCCGAGCCCC AGCCTTCCAC CTCCGCAGAC 
CAGCCTGTCA CCCCCAAACC CACGTCTCGG GCCCCTCGGG GCAGGACACC TAGGTCTTCC 
GCCAAGACTC CTGAGCCAGT TGTCTCCACA GCCTCTGAGC TCCAGCCTTC TGCCCTCACA 
GATGAGCCTG TCACTCCTGA GCTCACATCT AGGGCTACTC GGGGTCGGGC ACAGAGGTCC 
TCTGTCAAAA CCCCTGATCC AGTTACCACC ACAACACCTG AGCTCCAGCC TTCCACCTCC 
ACAGACCAGC TTGTCACCCC CAAACGCACA TCTCGGGCCC CTCGAGGCAG GACACGTAGG 
TTGTCTGCCA AGACTCCTGA ACCAGTTGTT CCCACAGCCT CTGAGCTCCA GCCTTCTGCC 
CCTGCAGACC AGCCTGTTGG TCCTTGGGCC ACTCAGTGTA GAAGACATAG GTCTTCTGTC 
AAGACCCCAG AACCAGTTGT CCCCACAGTC CCTGAACCTC AGCCTTCCAC TTCTAAAGAC 
CAGTCTGTCA CTCCTGAGCC CACATCTCAG GCCACTGAGA GCCAAACACA TAGGTCCTCT 
ATCAAGACAT CCCAGCCAAC GGAACCCACA GCCTCTGACC TCAAACCTTC CTCCCCCACA 
GACCAGCTTG TCACTCCCAA GGTCATAGCT CAGGGTGGTC CAAGCAGGAC AGGAAGGGCT 
TCCACAGCAA GTGCTGTGCT GGTTCCTACT ACCCCTGAAT TCCAGTCTCC AGTCCCGTCA 
GAACAGCCTC TTCCCCCTGA CCCCATCCCT GAAGTCAACT GCAGCAGGAG GCTGAGGGCC 
ACTAGGAAAC ATGGGTCTCC CACAGCTCAT GTTCATGAGC CCTGCACCGT ACCCCCTGAA 
CCTAACTCCC ACTCTTCAAG GAACCAAAGA CATGGGGCAG TGAAAGCAGC CAAGCCCCTT 
AGCACCATTC CTGAGCCTGC CTTTGCCCAG CTCCCCGAGG CACCGCCTCA CACTCCCCAG 
GTGCCAAAGG AGGAGGCAGC AGATGGATCA GGCTTCACCC CAGAGCCCCA GCCTAGGGCC 
TCTCAAAACC ACAAGAGGCC TTCAGCTACT GCACATTCAC CTCCACTTCA AAAACGGCTC 
CAGAGAGGGA GAGTCCCTCA GAAGGCAGCA TCCCTTAAGG AAGAAGAAAA TCCAGTAGCG 
AGGCCGAGGA AGGAAGAGGG TGTAGTGATT CCAGGTCCAG GCAAGAGAAA GAGAGAGCAG 
ACAGAGGAGG AGTCCCAGGG AAGACCGAGC CGCAGCCTGC GACGGACCAG ACCGATGCAG 
GAGTCCACGG CCCCCAAAGT GCTGTTCACC GGTGTGGTGG ATGCTCGCGG AGAGAGGACG 
GTGCTGGCCC TGGGGGGCAG TTTGGCCAGC TCGGTGGCCG AGGCTTCTCA CCTGGTGACT 
GATCGGATCC GCCGGACGGT CAAGTTTCTG TGTGCCCTGG GCCGGGGCAT CCCCATCCTC 
TCCCTGGCCT GGCTGCATGA GTCCCGCAAG GCAGGCTGCT TCTTGCCCCC GGACGAATAT 
TTGGTGACTG ATCCTGAGCA GGAGAAGAAC TTCGGCTTCA GCCTTCGGGA GGCCCTGAGC 
CGAGCTCAGG AGCGAAGGCT GCTGGAGGGC TATGAGATTC ATGTGACCCC CGGAGTCCAG 
CCACCGCCAC CTCAGATGGG AGAAATCATC AGCTGCTGTG GAGGCGCCAT CCTGCCCAGC 
ATGCCTCGGT CCTACAAGCC TCAGAGGTTC GTGATCACAT GTTCCCAGGA CTTTCCTCGA 
TGTGCTGTTC CATATCGGGT TGGGCTGCCT ATCCTCTCAC CCGAATTCCT GCTGACGGGA 
GTACTGAAGC AGGAAGTCAA GCCAGAGGCC TTTGCCTTCT CCACTGTGGA AATGTCATCC 
ACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012019795.1
CDS	816..6581
Translation	MEDTQILNWEVEEEEEVAESPNESLGYSLEPLAQLHIFSSSYGPEKDFPLYLGKNMIGRMPDCSVALPFSSISKQHAVIEISAWDKAPVLRDCGSLNGTQILRPPKVLSPGVSHRLRDRELILFADLPCQYHRLDVPRPFVSRGPLTVEETPRVQGGTQPSRLLLAEDSEEEVDSLLDKCVVKGPRTSSSATVVPESDEEGPSPAPDGPGPPSAFNLNSDTDEEESQESGAGEASSAARRGTAAETEQPEPVTAEIQIEKDQCSVKERNGDTEIERDVRNGVVPTGVILERSQPSGEDSDTDVSDESGPRRRLVGVRPKRAWSCNFIDSDTDGEDEGIPATPAVVPMKERQIFHEAGTQSPQAPGVARWQESPADGDTDIEEGEAPPDRSQASVVIDSNTDDEEEVSAALTLACLRESQAGKWNRDPDAEETRAQPVALLERSQASAGGDSDTDVEEEGLPVERRGMVPKGYMDREYSKKSQHPPRDSDTEGKEDESSPGVHLERSQASAQVEDEVPLGPAVALPEKRQVQGIVWPHHTDAEAEGDPAQLPVLRLEEARPPLAGDCELDSENTSAVRKSQLPAEKDAGTTWAAAVPEQDRALAIGTQGGSSTAPGEQDLLPVSREDLADMVVDIGTPGEPQPQREGAQTTTGREREPYGNRATDSGDNLHDSEDLDLPATQCFVDRKNQSLEAAPSMEDEPTQAFLFPLPQEPGPSCCSSQATGSLDESWEALATQPFCPRESEASETQTVVTLLDTHASWPSPSRTAQQEQHPESPVHAEPLGMEGREMQTVEKEMGTPRETAERVIPEGGPPQEETKKLPSEGEREDVTGEEELIGGIQGREKNQVFARDTQSQESDKKVKSASTGRGMEILKVEIETPKETQEKEREKQTLAGEIFESEAGKLVVERESEADGLEVKEPQELLDRSPQMGETEAGDQDQKGQASGLPSEPGAGAGDLQGLASDPVASGSQAGGGRGVPGSPRRQQRGDLNCEMPPAQKASRGDQESPDACLPPAAPEASAALPNSLIISQIQKHPAPQSLLFPSLAPLELPIPRTRQNETQEAPETPFSSELNSVHPEPKVRLQASSPVSSLPLEPHPTTPTGQPIALEPISGVSWSRTRSSFDVTASSVVPTAVALQPSISTDKPVTPKPTLRAPRGRAQRSSVKTPEPNVTTDQPVAPELTAMATRGRAQRSSVKIPKPDNPTTPKPQPSTSTDQPVIPKPTSRAPRGRTPRSSAKTPEPAVPTASELQPAAPKDQPVAPELTSRATRGRTQRSIKTSKPDMPTTPEPQPSTSADQPVTPKPTSRAPRGRTPRSSAKTPEPVVSTASELQPSALTDEPVTPELTSRATRGRAQRSSVKTPDPVTTTTPELQPSTSTDQLVTPKRTSRAPRGRTRRLSAKTPEPVVPTASELQPSAPADQPVGPWATQCRRHRSSVKTPEPVVPTVPEPQPSTSKDQSVTPEPTSQATESQTHRSSIKTSQPTEPTASDLKPSSPTDQLVTPKVIAQGGPSRTGRASTASAVLVPTTPEFQSPVPSEQPLPPDPIPEVNCSRRLRATRKHGSPTAHVHEPCTVPPEPNSHSSRNQRHGAVKAAKPLSTIPEPAFAQLPEAPPHTPQVPKEEAADGSGFTPEPQPRASQNHKRPSATAHSPPLQKRLQRGRVPQKAASLKEEENPVARPRKEEGVVIPGPGKRKREQTEEESQGRPSRSLRRTRPMQESTAPKVLFTGVVDARGERTVLALGGSLASSVAEASHLVTDRIRRTVKFLCALGRGIPILSLAWLHESRKAGCFLPPDEYLVTDPEQEKNFGFSLREALSRAQERRLLEGYEIHVTPGVQPPPPQMGEIISCCGGAILPSMPRSYKPQRFVITCSQDFPRCAVPYRVGLPILSPEFLLTGVLKQEVKPEAFAFSTVEMSST

Target ORF information:

RefSeq Version	XM_012164405.2
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon mediator of DNA damage checkpoint 1 (MDC1), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012164405.2

ORF Insert Sequence:

ATGGAAGACA CCCAGATTTT AAACTGGGAA GTTGAAGAAG AGGAGGAGGT TGCAGAGAGC 
CCCAATGAAT CTCTGGGGTA TAGCTTGGAG CCCCTAGCAC AGCTGCATAT CTTCAGTAGT 
TCCTATGGAC CAGAAAAAGA CTTCCCACTC TACCTTGGAA AGAACATGAT AGGCCGAATG 
CCTGATTGCT CTGTGGCCCT GCCCTTTTCA TCAATATCCA AACAACATGC AGTGATTGAA 
ATCTCTGCCT GGGACAAGGC GCCTGTCCTT CGGGATTGCG GGAGCCTCAA TGGCACTCAA 
ATCCTGCGGC CTCCTAAGGT CCTGAGCCCT GGGGTGAGTC ATCGTTTGCG GGACCGGGAG 
TTGATTCTCT TTGCTGACTT GCCCTGCCAG TACCATCGCT TGGATGTCCC CCGGCCTTTT 
GTCTCCCGGG GCCCTCTAAC TGTAGAGGAG ACACCCAGGG TACAGGGAGG AACTCAACCC 
TCCAGGCTTC TGTTGGCTGA GGACTCAGAG GAAGAAGTAG ATTCCCTTTT GGACAAGTGT 
GTGGTGAAAG GACCAAGGAC CTCCTCTTCG GCAACAGTCG TTCCAGAGAG TGATGAAGAA 
GGGCCTTCCC CTGCCCCAGA TGGGCCCGGG CCACCTTCTG CCTTCAACTT GAACAGCGAC 
ACGGATGAGG AAGAAAGTCA GGAATCAGGA GCAGGGGAGG CCTCTTCAGC TGCCAGAAGA 
GGTACCGCTG CAGAGACGGA ACAGCCTGAA CCTGTCACAG CCGAGATCCA GATTGAGAAG 
GATCAGTGTT CCGTGAAGGA GAGGAACGGG GACACAGAAA TCGAGAGGGA TGTGAGGAAT 
GGGGTGGTTC CGACTGGAGT GATTCTGGAG AGGAGCCAGC CTTCTGGGGA GGACAGTGAC 
ACAGATGTGA GTGATGAGAG TGGGCCTCGA AGAAGGCTTG TTGGAGTCCG TCCGAAAAGG 
GCCTGGTCTT GTAACTTCAT AGACAGTGAT ACCGATGGAG AGGACGAGGG GATCCCTGCT 
ACCCCAGCAG TGGTTCCCAT GAAGGAGAGG CAGATCTTCC ACGAAGCTGG TACACAGAGC 
CCCCAGGCAC CTGGTGTGGC ACGTTGGCAG GAGAGCCCAG CTGATGGTGA TACAGATATA 
GAGGAGGGGG AGGCCCCCCC AGACAGAAGC CAAGCCTCCG TGGTGATCGA CAGCAATACA 
GACGATGAGG AAGAAGTCTC AGCAGCACTG ACACTGGCAT GTCTAAGAGA GAGCCAGGCT 
GGTAAGTGGA ACCGAGATCC AGATGCAGAA GAGACCAGGG CCCAACCCGT GGCCCTTCTG 
GAGCGAAGCC AGGCCTCTGC TGGGGGAGAC AGTGACACAG ATGTGGAGGA AGAGGGGCTC 
CCAGTGGAAA GGAGGGGAAT GGTTCCCAAG GGTTACATGG ACAGGGAATA TTCAAAAAAG 
AGCCAGCACC CTCCCAGGGA CAGTGATACA GAGGGGAAGG AAGATGAGAG CTCACCGGGG 
GTCCACCTGG AGAGAAGCCA GGCCTCTGCA CAAGTGGAGG ATGAGGTCCC ACTGGGGCCA 
GCTGTTGCAC TTCCGGAGAA GCGTCAGGTA CAAGGCATAG TGTGGCCACA TCACACCGAT 
GCGGAGGCAG AAGGGGACCC GGCACAGCTC CCCGTGCTGC GTCTAGAGGA AGCCCGGCCT 
CCTCTAGCTG GGGACTGTGA ACTGGATTCG GAGAACACAT CTGCTGTCAG AAAGAGCCAG 
CTTCCGGCGG AAAAAGATGC TGGGACCACG TGGGCTGCAG CCGTTCCTGA ACAGGACAGA 
GCACTTGCCA TCGGGACCCA GGGTGGGTCA TCCACGGCAC CAGGGGAGCA AGACCTTCTC 
CCGGTCTCAA GGGAAGACCT AGCAGATATG GTGGTGGACA TAGGCACTCC AGGGGAGCCC 
CAACCGCAGA GAGAGGGGGC CCAGACCACC ACAGGAAGGG AGAGAGAACC ATATGGGAAT 
AGGGCCACAG ACTCTGGAGA CAACCTCCAC GATTCTGAAG ATCTGGACCT ACCAGCTACC 
CAGTGCTTTG TAGACAGAAA GAATCAGAGC CTGGAAGCAG CCCCCAGCAT GGAGGATGAG 
CCCACCCAGG CCTTCCTGTT TCCTCTGCCC CAAGAGCCTG GCCCTTCCTG TTGCAGCTCC 
CAGGCCACAG GTTCCTTGGA TGAGTCATGG GAGGCCTTGG CGACACAGCC ATTCTGTCCG 
AGAGAATCTG AGGCCTCCGA GACCCAAACC GTTGTCACCC TCCTTGACAC CCATGCATCT 
TGGCCCTCTC CATCTAGGAC AGCACAGCAA GAGCAACATC CAGAGAGCCC AGTCCATGCA 
GAGCCACTGG GGATGGAAGG CAGAGAGATG CAGACTGTGG AGAAAGAAAT GGGTACCCCA 
AGAGAAACAG CAGAGAGGGT GATCCCTGAG GGAGGGCCAC CGCAGGAGGA AACCAAGAAA 
CTGCCCTCAG AAGGAGAGAG GGAAGATGTG ACGGGAGAGG AAGAATTAAT CGGGGGGATA 
CAGGGCAGAG AAAAAAATCA GGTGTTTGCT AGAGATACTC AGAGCCAAGA ATCTGACAAA 
AAAGTGAAAA GTGCAAGTAC TGGAAGGGGA ATGGAGATTT TAAAGGTAGA AATTGAGACA 
CCCAAGGAAA CACAAGAGAA AGAGAGAGAA AAGCAGACTC TCGCAGGGGA AATATTTGAG 
AGTGAAGCAG GGAAACTGGT AGTAGAGAGA GAGAGTGAGG CAGATGGGTT AGAAGTCAAG 
GAACCCCAAG AGCTACTGGA CAGAAGCCCA CAGATGGGGG AGACAGAGGC GGGGGACCAG 
GACCAGAAAG GCCAAGCCTC TGGTCTGCCA TCAGAGCCTG GAGCAGGGGC AGGAGACCTT 
CAGGGACTTG CTTCAGACCC AGTAGCTTCT GGGAGCCAGG CAGGTGGAGG AAGGGGAGTC 
CCAGGGAGCC CCAGGAGGCA GCAGAGAGGT GACTTGAATT GCGAGATGCC ACCTGCTCAG 
AAGGCTTCCA GGGGTGATCA GGAATCCCCA GATGCTTGTC TGCCTCCTGC AGCGCCTGAA 
GCCTCAGCCG CACTCCCAAA TTCCCTCATC ATCTCTCAGA TCCAAAAACA TCCCGCACCT 
CAGTCCCTCC TTTTTCCCTC TCTAGCTCCT TTAGAACTGC CTATTCCCAG GACCAGACAA 
AATGAGACTC AGGAAGCTCC AGAGACTCCC TTCTCCTCAG AGCTGAACTC TGTCCACCCA 
GAACCCAAAG TCAGGCTCCA GGCGTCCTCT CCAGTTTCTT CTCTACCCCT TGAGCCCCAC 
CCTACCACCC CCACAGGCCA GCCTATTGCC CTTGAACCCA TATCTGGGGT CTCTTGGAGC 
AGGACACGTA GTTCCTTTGA TGTAACTGCC TCATCAGTTG TCCCCACAGC CGTTGCACTG 
CAGCCATCCA TCTCCACAGA CAAGCCTGTC ACCCCTAAGC CCACACTTCG GGCCCCTCGG 
GGCAGGGCAC AGAGGTCTTC TGTCAAAACC CCTGAACCCA ATGTCACCAC AGACCAGCCT 
GTTGCCCCTG AGCTCACAGC TATGGCAACT CGGGGCAGGG CACAGAGGTC TTCTGTCAAG 
ATTCCCAAAC CAGATAACCC CACAACACCC AAGCCCCAGC CTTCCACTTC CACAGACCAG 
CCTGTCATCC CCAAACCCAC ATCTCGGGCC CCTCGGGGCA GGACACCTAG GTCTTCTGCC 
AAGACTCCTG AACCAGCTGT CCCCACAGCC TCTGAGCTCC AGCCTGCTGC CCCTAAAGAC 
CAGCCTGTTG CTCCTGAGCT CACATCTAGA GCCACTCGGG GCAGGACACA GAGGTCTATC 
AAGACTTCCA AACCAGATAT GCCCACAACT CCCGAGCCCC AGCCTTCCAC CTCCGCAGAC 
CAGCCTGTCA CCCCCAAACC CACGTCTCGG GCCCCTCGGG GCAGGACACC TAGGTCTTCC 
GCCAAGACTC CTGAGCCAGT TGTCTCCACA GCCTCTGAGC TCCAGCCTTC TGCCCTCACA 
GATGAGCCTG TCACTCCTGA GCTCACATCT AGGGCTACTC GGGGTCGGGC ACAGAGGTCC 
TCTGTCAAAA CCCCTGATCC AGTTACCACC ACAACACCTG AGCTCCAGCC TTCCACCTCC 
ACAGACCAGC TTGTCACCCC CAAACGCACA TCTCGGGCCC CTCGAGGCAG GACACGTAGG 
TTGTCTGCCA AGACTCCTGA ACCAGTTGTT CCCACAGCCT CTGAGCTCCA GCCTTCTGCC 
CCTGCAGACC AGCCTGTTGG TCCTTGGGCC ACTCAGTGTA GAAGACATAG GTCTTCTGTC 
AAGACCCCAG AACCAGTTGT CCCCACAGTC CCTGAACCTC AGCCTTCCAC TTCTAAAGAC 
CAGTCTGTCA CTCCTGAGCC CACATCTCAG GCCACTGAGA GCCAAACACA TAGGTCCTCT 
ATCAAGACAT CCCAGCCAAC GGAACCCACA GCCTCTGACC TCAAACCTTC CTCCCCCACA 
GACCAGCTTG TCACTCCCAA GGTCATAGCT CAGGGTGGTC CAAGCAGGAC AGGAAGGGCT 
TCCACAGCAA GTGCTGTGCT GGTTCCTACT ACCCCTGAAT TCCAGTCTCC AGTCCCGTCA 
GAACAGCCTC TTCCCCCTGA CCCCATCCCT GAAGTCAACT GCAGCAGGAG GCTGAGGGCC 
ACTAGGAAAC ATGGGTCTCC CACAGCTCAT GTTCATGAGC CCTGCACCGT ACCCCCTGAA 
CCTAACTCCC ACTCTTCAAG GAACCAAAGA CATGGGGCAG TGAAAGCAGC CAAGCCCCTT 
AGCACCATTC CTGAGCCTGC CTTTGCCCAG CTCCCCGAGG CACCGCCTCA CACTCCCCAG 
GTGCCAAAGG AGGAGGCAGC AGATGGATCA GGCTTCACCC CAGAGCCCCA GCCTAGGGCC 
TCTCAAAACC ACAAGAGGCC TTCAGCTACT GCACATTCAC CTCCACTTCA AAAACGGCTC 
CAGAGAGGGA GAGTCCCTCA GAAGGCAGCA TCCCTTAAGG AAGAAGAAAA TCCAGTAGCG 
AGGCCGAGGA AGGAAGAGGG TGTAGTGATT CCAGGTCCAG GCAAGAGAAA GAGAGAGCAG 
ACAGAGGAGG AGTCCCAGGG AAGACCGAGC CGCAGCCTGC GACGGACCAG ACCGATGCAG 
GAGTCCACGG CCCCCAAAGT GCTGTTCACC GGTGTGGTGG ATGCTCGCGG AGAGAGGACG 
GTGCTGGCCC TGGGGGGCAG TTTGGCCAGC TCGGTGGCCG AGGCTTCTCA CCTGGTGACT 
GATCGGATCC GCCGGACGGT CAAGTTTCTG TGTGCCCTGG GCCGGGGCAT CCCCATCCTC 
TCCCTGGCCT GGCTGCATGA GTCCCGCAAG GCAGGCTGCT TCTTGCCCCC GGACGAATAT 
TTGGTGACTG ATCCTGAGCA GGAGAAGAAC TTCGGCTTCA GCCTTCGGGA GGCCCTGAGC 
CGAGCTCAGG AGCGAAGGCT GCTGGAGGGC TATGAGATTC ATGTGACCCC CGGAGTCCAG 
CCACCGCCAC CTCAGATGGG AGAAATCATC AGCTGCTGTG GAGGCGCCAT CCTGCCCAGC 
ATGCCTCGGT CCTACAAGCC TCAGAGGTTC GTGATCACAT GTTCCCAGGA CTTTCCTCGA 
TGTGCTGTTC CATATCGGGT TGGGCTGCCT ATCCTCTCAC CCGAATTCCT GCTGACGGGA 
GTACTGAAGC AGGAAGTCAA GCCAGAGGCC TTTGCCTTCT CCACTGTGGA AATGTCATCC 
ACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MDC1 ( XM_012164407.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_012019797.1 Ovis aries musimon mediator of DNA damage checkpoint 1 (MDC1), transcript variant X16, mRNA.

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CloneID	OOv35150
Clone ID Related Accession (Same CDS sequence)	XM_012164405.2 , XM_012164407.2 , XM_012164406.2 , XM_012164404.2
Accession Version	XM_012164407.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5766bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	mediator of DNA damage checkpoint protein 1 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011943095.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012164407.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGACA CCCAGATTTT AAACTGGGAA GTTGAAGAAG AGGAGGAGGT TGCAGAGAGC 
CCCAATGAAT CTCTGGGGTA TAGCTTGGAG CCCCTAGCAC AGCTGCATAT CTTCAGTAGT 
TCCTATGGAC CAGAAAAAGA CTTCCCACTC TACCTTGGAA AGAACATGAT AGGCCGAATG 
CCTGATTGCT CTGTGGCCCT GCCCTTTTCA TCAATATCCA AACAACATGC AGTGATTGAA 
ATCTCTGCCT GGGACAAGGC GCCTGTCCTT CGGGATTGCG GGAGCCTCAA TGGCACTCAA 
ATCCTGCGGC CTCCTAAGGT CCTGAGCCCT GGGGTGAGTC ATCGTTTGCG GGACCGGGAG 
TTGATTCTCT TTGCTGACTT GCCCTGCCAG TACCATCGCT TGGATGTCCC CCGGCCTTTT 
GTCTCCCGGG GCCCTCTAAC TGTAGAGGAG ACACCCAGGG TACAGGGAGG AACTCAACCC 
TCCAGGCTTC TGTTGGCTGA GGACTCAGAG GAAGAAGTAG ATTCCCTTTT GGACAAGTGT 
GTGGTGAAAG GACCAAGGAC CTCCTCTTCG GCAACAGTCG TTCCAGAGAG TGATGAAGAA 
GGGCCTTCCC CTGCCCCAGA TGGGCCCGGG CCACCTTCTG CCTTCAACTT GAACAGCGAC 
ACGGATGAGG AAGAAAGTCA GGAATCAGGA GCAGGGGAGG CCTCTTCAGC TGCCAGAAGA 
GGTACCGCTG CAGAGACGGA ACAGCCTGAA CCTGTCACAG CCGAGATCCA GATTGAGAAG 
GATCAGTGTT CCGTGAAGGA GAGGAACGGG GACACAGAAA TCGAGAGGGA TGTGAGGAAT 
GGGGTGGTTC CGACTGGAGT GATTCTGGAG AGGAGCCAGC CTTCTGGGGA GGACAGTGAC 
ACAGATGTGA GTGATGAGAG TGGGCCTCGA AGAAGGCTTG TTGGAGTCCG TCCGAAAAGG 
GCCTGGTCTT GTAACTTCAT AGACAGTGAT ACCGATGGAG AGGACGAGGG GATCCCTGCT 
ACCCCAGCAG TGGTTCCCAT GAAGGAGAGG CAGATCTTCC ACGAAGCTGG TACACAGAGC 
CCCCAGGCAC CTGGTGTGGC ACGTTGGCAG GAGAGCCCAG CTGATGGTGA TACAGATATA 
GAGGAGGGGG AGGCCCCCCC AGACAGAAGC CAAGCCTCCG TGGTGATCGA CAGCAATACA 
GACGATGAGG AAGAAGTCTC AGCAGCACTG ACACTGGCAT GTCTAAGAGA GAGCCAGGCT 
GGTAAGTGGA ACCGAGATCC AGATGCAGAA GAGACCAGGG CCCAACCCGT GGCCCTTCTG 
GAGCGAAGCC AGGCCTCTGC TGGGGGAGAC AGTGACACAG ATGTGGAGGA AGAGGGGCTC 
CCAGTGGAAA GGAGGGGAAT GGTTCCCAAG GGTTACATGG ACAGGGAATA TTCAAAAAAG 
AGCCAGCACC CTCCCAGGGA CAGTGATACA GAGGGGAAGG AAGATGAGAG CTCACCGGGG 
GTCCACCTGG AGAGAAGCCA GGCCTCTGCA CAAGTGGAGG ATGAGGTCCC ACTGGGGCCA 
GCTGTTGCAC TTCCGGAGAA GCGTCAGGTA CAAGGCATAG TGTGGCCACA TCACACCGAT 
GCGGAGGCAG AAGGGGACCC GGCACAGCTC CCCGTGCTGC GTCTAGAGGA AGCCCGGCCT 
CCTCTAGCTG GGGACTGTGA ACTGGATTCG GAGAACACAT CTGCTGTCAG AAAGAGCCAG 
CTTCCGGCGG AAAAAGATGC TGGGACCACG TGGGCTGCAG CCGTTCCTGA ACAGGACAGA 
GCACTTGCCA TCGGGACCCA GGGTGGGTCA TCCACGGCAC CAGGGGAGCA AGACCTTCTC 
CCGGTCTCAA GGGAAGACCT AGCAGATATG GTGGTGGACA TAGGCACTCC AGGGGAGCCC 
CAACCGCAGA GAGAGGGGGC CCAGACCACC ACAGGAAGGG AGAGAGAACC ATATGGGAAT 
AGGGCCACAG ACTCTGGAGA CAACCTCCAC GATTCTGAAG ATCTGGACCT ACCAGCTACC 
CAGTGCTTTG TAGACAGAAA GAATCAGAGC CTGGAAGCAG CCCCCAGCAT GGAGGATGAG 
CCCACCCAGG CCTTCCTGTT TCCTCTGCCC CAAGAGCCTG GCCCTTCCTG TTGCAGCTCC 
CAGGCCACAG GTTCCTTGGA TGAGTCATGG GAGGCCTTGG CGACACAGCC ATTCTGTCCG 
AGAGAATCTG AGGCCTCCGA GACCCAAACC GTTGTCACCC TCCTTGACAC CCATGCATCT 
TGGCCCTCTC CATCTAGGAC AGCACAGCAA GAGCAACATC CAGAGAGCCC AGTCCATGCA 
GAGCCACTGG GGATGGAAGG CAGAGAGATG CAGACTGTGG AGAAAGAAAT GGGTACCCCA 
AGAGAAACAG CAGAGAGGGT GATCCCTGAG GGAGGGCCAC CGCAGGAGGA AACCAAGAAA 
CTGCCCTCAG AAGGAGAGAG GGAAGATGTG ACGGGAGAGG AAGAATTAAT CGGGGGGATA 
CAGGGCAGAG AAAAAAATCA GGTGTTTGCT AGAGATACTC AGAGCCAAGA ATCTGACAAA 
AAAGTGAAAA GTGCAAGTAC TGGAAGGGGA ATGGAGATTT TAAAGGTAGA AATTGAGACA 
CCCAAGGAAA CACAAGAGAA AGAGAGAGAA AAGCAGACTC TCGCAGGGGA AATATTTGAG 
AGTGAAGCAG GGAAACTGGT AGTAGAGAGA GAGAGTGAGG CAGATGGGTT AGAAGTCAAG 
GAACCCCAAG AGCTACTGGA CAGAAGCCCA CAGATGGGGG AGACAGAGGC GGGGGACCAG 
GACCAGAAAG GCCAAGCCTC TGGTCTGCCA TCAGAGCCTG GAGCAGGGGC AGGAGACCTT 
CAGGGACTTG CTTCAGACCC AGTAGCTTCT GGGAGCCAGG CAGGTGGAGG AAGGGGAGTC 
CCAGGGAGCC CCAGGAGGCA GCAGAGAGGT GACTTGAATT GCGAGATGCC ACCTGCTCAG 
AAGGCTTCCA GGGGTGATCA GGAATCCCCA GATGCTTGTC TGCCTCCTGC AGCGCCTGAA 
GCCTCAGCCG CACTCCCAAA TTCCCTCATC ATCTCTCAGA TCCAAAAACA TCCCGCACCT 
CAGTCCCTCC TTTTTCCCTC TCTAGCTCCT TTAGAACTGC CTATTCCCAG GACCAGACAA 
AATGAGACTC AGGAAGCTCC AGAGACTCCC TTCTCCTCAG AGCTGAACTC TGTCCACCCA 
GAACCCAAAG TCAGGCTCCA GGCGTCCTCT CCAGTTTCTT CTCTACCCCT TGAGCCCCAC 
CCTACCACCC CCACAGGCCA GCCTATTGCC CTTGAACCCA TATCTGGGGT CTCTTGGAGC 
AGGACACGTA GTTCCTTTGA TGTAACTGCC TCATCAGTTG TCCCCACAGC CGTTGCACTG 
CAGCCATCCA TCTCCACAGA CAAGCCTGTC ACCCCTAAGC CCACACTTCG GGCCCCTCGG 
GGCAGGGCAC AGAGGTCTTC TGTCAAAACC CCTGAACCCA ATGTCACCAC AGACCAGCCT 
GTTGCCCCTG AGCTCACAGC TATGGCAACT CGGGGCAGGG CACAGAGGTC TTCTGTCAAG 
ATTCCCAAAC CAGATAACCC CACAACACCC AAGCCCCAGC CTTCCACTTC CACAGACCAG 
CCTGTCATCC CCAAACCCAC ATCTCGGGCC CCTCGGGGCA GGACACCTAG GTCTTCTGCC 
AAGACTCCTG AACCAGCTGT CCCCACAGCC TCTGAGCTCC AGCCTGCTGC CCCTAAAGAC 
CAGCCTGTTG CTCCTGAGCT CACATCTAGA GCCACTCGGG GCAGGACACA GAGGTCTATC 
AAGACTTCCA AACCAGATAT GCCCACAACT CCCGAGCCCC AGCCTTCCAC CTCCGCAGAC 
CAGCCTGTCA CCCCCAAACC CACGTCTCGG GCCCCTCGGG GCAGGACACC TAGGTCTTCC 
GCCAAGACTC CTGAGCCAGT TGTCTCCACA GCCTCTGAGC TCCAGCCTTC TGCCCTCACA 
GATGAGCCTG TCACTCCTGA GCTCACATCT AGGGCTACTC GGGGTCGGGC ACAGAGGTCC 
TCTGTCAAAA CCCCTGATCC AGTTACCACC ACAACACCTG AGCTCCAGCC TTCCACCTCC 
ACAGACCAGC TTGTCACCCC CAAACGCACA TCTCGGGCCC CTCGAGGCAG GACACGTAGG 
TTGTCTGCCA AGACTCCTGA ACCAGTTGTT CCCACAGCCT CTGAGCTCCA GCCTTCTGCC 
CCTGCAGACC AGCCTGTTGG TCCTTGGGCC ACTCAGTGTA GAAGACATAG GTCTTCTGTC 
AAGACCCCAG AACCAGTTGT CCCCACAGTC CCTGAACCTC AGCCTTCCAC TTCTAAAGAC 
CAGTCTGTCA CTCCTGAGCC CACATCTCAG GCCACTGAGA GCCAAACACA TAGGTCCTCT 
ATCAAGACAT CCCAGCCAAC GGAACCCACA GCCTCTGACC TCAAACCTTC CTCCCCCACA 
GACCAGCTTG TCACTCCCAA GGTCATAGCT CAGGGTGGTC CAAGCAGGAC AGGAAGGGCT 
TCCACAGCAA GTGCTGTGCT GGTTCCTACT ACCCCTGAAT TCCAGTCTCC AGTCCCGTCA 
GAACAGCCTC TTCCCCCTGA CCCCATCCCT GAAGTCAACT GCAGCAGGAG GCTGAGGGCC 
ACTAGGAAAC ATGGGTCTCC CACAGCTCAT GTTCATGAGC CCTGCACCGT ACCCCCTGAA 
CCTAACTCCC ACTCTTCAAG GAACCAAAGA CATGGGGCAG TGAAAGCAGC CAAGCCCCTT 
AGCACCATTC CTGAGCCTGC CTTTGCCCAG CTCCCCGAGG CACCGCCTCA CACTCCCCAG 
GTGCCAAAGG AGGAGGCAGC AGATGGATCA GGCTTCACCC CAGAGCCCCA GCCTAGGGCC 
TCTCAAAACC ACAAGAGGCC TTCAGCTACT GCACATTCAC CTCCACTTCA AAAACGGCTC 
CAGAGAGGGA GAGTCCCTCA GAAGGCAGCA TCCCTTAAGG AAGAAGAAAA TCCAGTAGCG 
AGGCCGAGGA AGGAAGAGGG TGTAGTGATT CCAGGTCCAG GCAAGAGAAA GAGAGAGCAG 
ACAGAGGAGG AGTCCCAGGG AAGACCGAGC CGCAGCCTGC GACGGACCAG ACCGATGCAG 
GAGTCCACGG CCCCCAAAGT GCTGTTCACC GGTGTGGTGG ATGCTCGCGG AGAGAGGACG 
GTGCTGGCCC TGGGGGGCAG TTTGGCCAGC TCGGTGGCCG AGGCTTCTCA CCTGGTGACT 
GATCGGATCC GCCGGACGGT CAAGTTTCTG TGTGCCCTGG GCCGGGGCAT CCCCATCCTC 
TCCCTGGCCT GGCTGCATGA GTCCCGCAAG GCAGGCTGCT TCTTGCCCCC GGACGAATAT 
TTGGTGACTG ATCCTGAGCA GGAGAAGAAC TTCGGCTTCA GCCTTCGGGA GGCCCTGAGC 
CGAGCTCAGG AGCGAAGGCT GCTGGAGGGC TATGAGATTC ATGTGACCCC CGGAGTCCAG 
CCACCGCCAC CTCAGATGGG AGAAATCATC AGCTGCTGTG GAGGCGCCAT CCTGCCCAGC 
ATGCCTCGGT CCTACAAGCC TCAGAGGTTC GTGATCACAT GTTCCCAGGA CTTTCCTCGA 
TGTGCTGTTC CATATCGGGT TGGGCTGCCT ATCCTCTCAC CCGAATTCCT GCTGACGGGA 
GTACTGAAGC AGGAAGTCAA GCCAGAGGCC TTTGCCTTCT CCACTGTGGA AATGTCATCC 
ACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012019797.1
CDS	189..5954
Translation	MEDTQILNWEVEEEEEVAESPNESLGYSLEPLAQLHIFSSSYGPEKDFPLYLGKNMIGRMPDCSVALPFSSISKQHAVIEISAWDKAPVLRDCGSLNGTQILRPPKVLSPGVSHRLRDRELILFADLPCQYHRLDVPRPFVSRGPLTVEETPRVQGGTQPSRLLLAEDSEEEVDSLLDKCVVKGPRTSSSATVVPESDEEGPSPAPDGPGPPSAFNLNSDTDEEESQESGAGEASSAARRGTAAETEQPEPVTAEIQIEKDQCSVKERNGDTEIERDVRNGVVPTGVILERSQPSGEDSDTDVSDESGPRRRLVGVRPKRAWSCNFIDSDTDGEDEGIPATPAVVPMKERQIFHEAGTQSPQAPGVARWQESPADGDTDIEEGEAPPDRSQASVVIDSNTDDEEEVSAALTLACLRESQAGKWNRDPDAEETRAQPVALLERSQASAGGDSDTDVEEEGLPVERRGMVPKGYMDREYSKKSQHPPRDSDTEGKEDESSPGVHLERSQASAQVEDEVPLGPAVALPEKRQVQGIVWPHHTDAEAEGDPAQLPVLRLEEARPPLAGDCELDSENTSAVRKSQLPAEKDAGTTWAAAVPEQDRALAIGTQGGSSTAPGEQDLLPVSREDLADMVVDIGTPGEPQPQREGAQTTTGREREPYGNRATDSGDNLHDSEDLDLPATQCFVDRKNQSLEAAPSMEDEPTQAFLFPLPQEPGPSCCSSQATGSLDESWEALATQPFCPRESEASETQTVVTLLDTHASWPSPSRTAQQEQHPESPVHAEPLGMEGREMQTVEKEMGTPRETAERVIPEGGPPQEETKKLPSEGEREDVTGEEELIGGIQGREKNQVFARDTQSQESDKKVKSASTGRGMEILKVEIETPKETQEKEREKQTLAGEIFESEAGKLVVERESEADGLEVKEPQELLDRSPQMGETEAGDQDQKGQASGLPSEPGAGAGDLQGLASDPVASGSQAGGGRGVPGSPRRQQRGDLNCEMPPAQKASRGDQESPDACLPPAAPEASAALPNSLIISQIQKHPAPQSLLFPSLAPLELPIPRTRQNETQEAPETPFSSELNSVHPEPKVRLQASSPVSSLPLEPHPTTPTGQPIALEPISGVSWSRTRSSFDVTASSVVPTAVALQPSISTDKPVTPKPTLRAPRGRAQRSSVKTPEPNVTTDQPVAPELTAMATRGRAQRSSVKIPKPDNPTTPKPQPSTSTDQPVIPKPTSRAPRGRTPRSSAKTPEPAVPTASELQPAAPKDQPVAPELTSRATRGRTQRSIKTSKPDMPTTPEPQPSTSADQPVTPKPTSRAPRGRTPRSSAKTPEPVVSTASELQPSALTDEPVTPELTSRATRGRAQRSSVKTPDPVTTTTPELQPSTSTDQLVTPKRTSRAPRGRTRRLSAKTPEPVVPTASELQPSAPADQPVGPWATQCRRHRSSVKTPEPVVPTVPEPQPSTSKDQSVTPEPTSQATESQTHRSSIKTSQPTEPTASDLKPSSPTDQLVTPKVIAQGGPSRTGRASTASAVLVPTTPEFQSPVPSEQPLPPDPIPEVNCSRRLRATRKHGSPTAHVHEPCTVPPEPNSHSSRNQRHGAVKAAKPLSTIPEPAFAQLPEAPPHTPQVPKEEAADGSGFTPEPQPRASQNHKRPSATAHSPPLQKRLQRGRVPQKAASLKEEENPVARPRKEEGVVIPGPGKRKREQTEEESQGRPSRSLRRTRPMQESTAPKVLFTGVVDARGERTVLALGGSLASSVAEASHLVTDRIRRTVKFLCALGRGIPILSLAWLHESRKAGCFLPPDEYLVTDPEQEKNFGFSLREALSRAQERRLLEGYEIHVTPGVQPPPPQMGEIISCCGGAILPSMPRSYKPQRFVITCSQDFPRCAVPYRVGLPILSPEFLLTGVLKQEVKPEAFAFSTVEMSST

Target ORF information:

RefSeq Version	XM_012164407.2
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon mediator of DNA damage checkpoint 1 (MDC1), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012164407.2

ORF Insert Sequence:

ATGGAAGACA CCCAGATTTT AAACTGGGAA GTTGAAGAAG AGGAGGAGGT TGCAGAGAGC 
CCCAATGAAT CTCTGGGGTA TAGCTTGGAG CCCCTAGCAC AGCTGCATAT CTTCAGTAGT 
TCCTATGGAC CAGAAAAAGA CTTCCCACTC TACCTTGGAA AGAACATGAT AGGCCGAATG 
CCTGATTGCT CTGTGGCCCT GCCCTTTTCA TCAATATCCA AACAACATGC AGTGATTGAA 
ATCTCTGCCT GGGACAAGGC GCCTGTCCTT CGGGATTGCG GGAGCCTCAA TGGCACTCAA 
ATCCTGCGGC CTCCTAAGGT CCTGAGCCCT GGGGTGAGTC ATCGTTTGCG GGACCGGGAG 
TTGATTCTCT TTGCTGACTT GCCCTGCCAG TACCATCGCT TGGATGTCCC CCGGCCTTTT 
GTCTCCCGGG GCCCTCTAAC TGTAGAGGAG ACACCCAGGG TACAGGGAGG AACTCAACCC 
TCCAGGCTTC TGTTGGCTGA GGACTCAGAG GAAGAAGTAG ATTCCCTTTT GGACAAGTGT 
GTGGTGAAAG GACCAAGGAC CTCCTCTTCG GCAACAGTCG TTCCAGAGAG TGATGAAGAA 
GGGCCTTCCC CTGCCCCAGA TGGGCCCGGG CCACCTTCTG CCTTCAACTT GAACAGCGAC 
ACGGATGAGG AAGAAAGTCA GGAATCAGGA GCAGGGGAGG CCTCTTCAGC TGCCAGAAGA 
GGTACCGCTG CAGAGACGGA ACAGCCTGAA CCTGTCACAG CCGAGATCCA GATTGAGAAG 
GATCAGTGTT CCGTGAAGGA GAGGAACGGG GACACAGAAA TCGAGAGGGA TGTGAGGAAT 
GGGGTGGTTC CGACTGGAGT GATTCTGGAG AGGAGCCAGC CTTCTGGGGA GGACAGTGAC 
ACAGATGTGA GTGATGAGAG TGGGCCTCGA AGAAGGCTTG TTGGAGTCCG TCCGAAAAGG 
GCCTGGTCTT GTAACTTCAT AGACAGTGAT ACCGATGGAG AGGACGAGGG GATCCCTGCT 
ACCCCAGCAG TGGTTCCCAT GAAGGAGAGG CAGATCTTCC ACGAAGCTGG TACACAGAGC 
CCCCAGGCAC CTGGTGTGGC ACGTTGGCAG GAGAGCCCAG CTGATGGTGA TACAGATATA 
GAGGAGGGGG AGGCCCCCCC AGACAGAAGC CAAGCCTCCG TGGTGATCGA CAGCAATACA 
GACGATGAGG AAGAAGTCTC AGCAGCACTG ACACTGGCAT GTCTAAGAGA GAGCCAGGCT 
GGTAAGTGGA ACCGAGATCC AGATGCAGAA GAGACCAGGG CCCAACCCGT GGCCCTTCTG 
GAGCGAAGCC AGGCCTCTGC TGGGGGAGAC AGTGACACAG ATGTGGAGGA AGAGGGGCTC 
CCAGTGGAAA GGAGGGGAAT GGTTCCCAAG GGTTACATGG ACAGGGAATA TTCAAAAAAG 
AGCCAGCACC CTCCCAGGGA CAGTGATACA GAGGGGAAGG AAGATGAGAG CTCACCGGGG 
GTCCACCTGG AGAGAAGCCA GGCCTCTGCA CAAGTGGAGG ATGAGGTCCC ACTGGGGCCA 
GCTGTTGCAC TTCCGGAGAA GCGTCAGGTA CAAGGCATAG TGTGGCCACA TCACACCGAT 
GCGGAGGCAG AAGGGGACCC GGCACAGCTC CCCGTGCTGC GTCTAGAGGA AGCCCGGCCT 
CCTCTAGCTG GGGACTGTGA ACTGGATTCG GAGAACACAT CTGCTGTCAG AAAGAGCCAG 
CTTCCGGCGG AAAAAGATGC TGGGACCACG TGGGCTGCAG CCGTTCCTGA ACAGGACAGA 
GCACTTGCCA TCGGGACCCA GGGTGGGTCA TCCACGGCAC CAGGGGAGCA AGACCTTCTC 
CCGGTCTCAA GGGAAGACCT AGCAGATATG GTGGTGGACA TAGGCACTCC AGGGGAGCCC 
CAACCGCAGA GAGAGGGGGC CCAGACCACC ACAGGAAGGG AGAGAGAACC ATATGGGAAT 
AGGGCCACAG ACTCTGGAGA CAACCTCCAC GATTCTGAAG ATCTGGACCT ACCAGCTACC 
CAGTGCTTTG TAGACAGAAA GAATCAGAGC CTGGAAGCAG CCCCCAGCAT GGAGGATGAG 
CCCACCCAGG CCTTCCTGTT TCCTCTGCCC CAAGAGCCTG GCCCTTCCTG TTGCAGCTCC 
CAGGCCACAG GTTCCTTGGA TGAGTCATGG GAGGCCTTGG CGACACAGCC ATTCTGTCCG 
AGAGAATCTG AGGCCTCCGA GACCCAAACC GTTGTCACCC TCCTTGACAC CCATGCATCT 
TGGCCCTCTC CATCTAGGAC AGCACAGCAA GAGCAACATC CAGAGAGCCC AGTCCATGCA 
GAGCCACTGG GGATGGAAGG CAGAGAGATG CAGACTGTGG AGAAAGAAAT GGGTACCCCA 
AGAGAAACAG CAGAGAGGGT GATCCCTGAG GGAGGGCCAC CGCAGGAGGA AACCAAGAAA 
CTGCCCTCAG AAGGAGAGAG GGAAGATGTG ACGGGAGAGG AAGAATTAAT CGGGGGGATA 
CAGGGCAGAG AAAAAAATCA GGTGTTTGCT AGAGATACTC AGAGCCAAGA ATCTGACAAA 
AAAGTGAAAA GTGCAAGTAC TGGAAGGGGA ATGGAGATTT TAAAGGTAGA AATTGAGACA 
CCCAAGGAAA CACAAGAGAA AGAGAGAGAA AAGCAGACTC TCGCAGGGGA AATATTTGAG 
AGTGAAGCAG GGAAACTGGT AGTAGAGAGA GAGAGTGAGG CAGATGGGTT AGAAGTCAAG 
GAACCCCAAG AGCTACTGGA CAGAAGCCCA CAGATGGGGG AGACAGAGGC GGGGGACCAG 
GACCAGAAAG GCCAAGCCTC TGGTCTGCCA TCAGAGCCTG GAGCAGGGGC AGGAGACCTT 
CAGGGACTTG CTTCAGACCC AGTAGCTTCT GGGAGCCAGG CAGGTGGAGG AAGGGGAGTC 
CCAGGGAGCC CCAGGAGGCA GCAGAGAGGT GACTTGAATT GCGAGATGCC ACCTGCTCAG 
AAGGCTTCCA GGGGTGATCA GGAATCCCCA GATGCTTGTC TGCCTCCTGC AGCGCCTGAA 
GCCTCAGCCG CACTCCCAAA TTCCCTCATC ATCTCTCAGA TCCAAAAACA TCCCGCACCT 
CAGTCCCTCC TTTTTCCCTC TCTAGCTCCT TTAGAACTGC CTATTCCCAG GACCAGACAA 
AATGAGACTC AGGAAGCTCC AGAGACTCCC TTCTCCTCAG AGCTGAACTC TGTCCACCCA 
GAACCCAAAG TCAGGCTCCA GGCGTCCTCT CCAGTTTCTT CTCTACCCCT TGAGCCCCAC 
CCTACCACCC CCACAGGCCA GCCTATTGCC CTTGAACCCA TATCTGGGGT CTCTTGGAGC 
AGGACACGTA GTTCCTTTGA TGTAACTGCC TCATCAGTTG TCCCCACAGC CGTTGCACTG 
CAGCCATCCA TCTCCACAGA CAAGCCTGTC ACCCCTAAGC CCACACTTCG GGCCCCTCGG 
GGCAGGGCAC AGAGGTCTTC TGTCAAAACC CCTGAACCCA ATGTCACCAC AGACCAGCCT 
GTTGCCCCTG AGCTCACAGC TATGGCAACT CGGGGCAGGG CACAGAGGTC TTCTGTCAAG 
ATTCCCAAAC CAGATAACCC CACAACACCC AAGCCCCAGC CTTCCACTTC CACAGACCAG 
CCTGTCATCC CCAAACCCAC ATCTCGGGCC CCTCGGGGCA GGACACCTAG GTCTTCTGCC 
AAGACTCCTG AACCAGCTGT CCCCACAGCC TCTGAGCTCC AGCCTGCTGC CCCTAAAGAC 
CAGCCTGTTG CTCCTGAGCT CACATCTAGA GCCACTCGGG GCAGGACACA GAGGTCTATC 
AAGACTTCCA AACCAGATAT GCCCACAACT CCCGAGCCCC AGCCTTCCAC CTCCGCAGAC 
CAGCCTGTCA CCCCCAAACC CACGTCTCGG GCCCCTCGGG GCAGGACACC TAGGTCTTCC 
GCCAAGACTC CTGAGCCAGT TGTCTCCACA GCCTCTGAGC TCCAGCCTTC TGCCCTCACA 
GATGAGCCTG TCACTCCTGA GCTCACATCT AGGGCTACTC GGGGTCGGGC ACAGAGGTCC 
TCTGTCAAAA CCCCTGATCC AGTTACCACC ACAACACCTG AGCTCCAGCC TTCCACCTCC 
ACAGACCAGC TTGTCACCCC CAAACGCACA TCTCGGGCCC CTCGAGGCAG GACACGTAGG 
TTGTCTGCCA AGACTCCTGA ACCAGTTGTT CCCACAGCCT CTGAGCTCCA GCCTTCTGCC 
CCTGCAGACC AGCCTGTTGG TCCTTGGGCC ACTCAGTGTA GAAGACATAG GTCTTCTGTC 
AAGACCCCAG AACCAGTTGT CCCCACAGTC CCTGAACCTC AGCCTTCCAC TTCTAAAGAC 
CAGTCTGTCA CTCCTGAGCC CACATCTCAG GCCACTGAGA GCCAAACACA TAGGTCCTCT 
ATCAAGACAT CCCAGCCAAC GGAACCCACA GCCTCTGACC TCAAACCTTC CTCCCCCACA 
GACCAGCTTG TCACTCCCAA GGTCATAGCT CAGGGTGGTC CAAGCAGGAC AGGAAGGGCT 
TCCACAGCAA GTGCTGTGCT GGTTCCTACT ACCCCTGAAT TCCAGTCTCC AGTCCCGTCA 
GAACAGCCTC TTCCCCCTGA CCCCATCCCT GAAGTCAACT GCAGCAGGAG GCTGAGGGCC 
ACTAGGAAAC ATGGGTCTCC CACAGCTCAT GTTCATGAGC CCTGCACCGT ACCCCCTGAA 
CCTAACTCCC ACTCTTCAAG GAACCAAAGA CATGGGGCAG TGAAAGCAGC CAAGCCCCTT 
AGCACCATTC CTGAGCCTGC CTTTGCCCAG CTCCCCGAGG CACCGCCTCA CACTCCCCAG 
GTGCCAAAGG AGGAGGCAGC AGATGGATCA GGCTTCACCC CAGAGCCCCA GCCTAGGGCC 
TCTCAAAACC ACAAGAGGCC TTCAGCTACT GCACATTCAC CTCCACTTCA AAAACGGCTC 
CAGAGAGGGA GAGTCCCTCA GAAGGCAGCA TCCCTTAAGG AAGAAGAAAA TCCAGTAGCG 
AGGCCGAGGA AGGAAGAGGG TGTAGTGATT CCAGGTCCAG GCAAGAGAAA GAGAGAGCAG 
ACAGAGGAGG AGTCCCAGGG AAGACCGAGC CGCAGCCTGC GACGGACCAG ACCGATGCAG 
GAGTCCACGG CCCCCAAAGT GCTGTTCACC GGTGTGGTGG ATGCTCGCGG AGAGAGGACG 
GTGCTGGCCC TGGGGGGCAG TTTGGCCAGC TCGGTGGCCG AGGCTTCTCA CCTGGTGACT 
GATCGGATCC GCCGGACGGT CAAGTTTCTG TGTGCCCTGG GCCGGGGCAT CCCCATCCTC 
TCCCTGGCCT GGCTGCATGA GTCCCGCAAG GCAGGCTGCT TCTTGCCCCC GGACGAATAT 
TTGGTGACTG ATCCTGAGCA GGAGAAGAAC TTCGGCTTCA GCCTTCGGGA GGCCCTGAGC 
CGAGCTCAGG AGCGAAGGCT GCTGGAGGGC TATGAGATTC ATGTGACCCC CGGAGTCCAG 
CCACCGCCAC CTCAGATGGG AGAAATCATC AGCTGCTGTG GAGGCGCCAT CCTGCCCAGC 
ATGCCTCGGT CCTACAAGCC TCAGAGGTTC GTGATCACAT GTTCCCAGGA CTTTCCTCGA 
TGTGCTGTTC CATATCGGGT TGGGCTGCCT ATCCTCTCAC CCGAATTCCT GCTGACGGGA 
GTACTGAAGC AGGAAGTCAA GCCAGAGGCC TTTGCCTTCT CCACTGTGGA AATGTCATCC 
ACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MDC1 ( XM_012164406.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_012019796.1 Ovis aries musimon mediator of DNA damage checkpoint 1 (MDC1), transcript variant X15, mRNA.

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CloneID	OOv35150
Clone ID Related Accession (Same CDS sequence)	XM_012164405.2 , XM_012164407.2 , XM_012164406.2 , XM_012164404.2
Accession Version	XM_012164406.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5766bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	mediator of DNA damage checkpoint protein 1 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011943095.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012164406.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGACA CCCAGATTTT AAACTGGGAA GTTGAAGAAG AGGAGGAGGT TGCAGAGAGC 
CCCAATGAAT CTCTGGGGTA TAGCTTGGAG CCCCTAGCAC AGCTGCATAT CTTCAGTAGT 
TCCTATGGAC CAGAAAAAGA CTTCCCACTC TACCTTGGAA AGAACATGAT AGGCCGAATG 
CCTGATTGCT CTGTGGCCCT GCCCTTTTCA TCAATATCCA AACAACATGC AGTGATTGAA 
ATCTCTGCCT GGGACAAGGC GCCTGTCCTT CGGGATTGCG GGAGCCTCAA TGGCACTCAA 
ATCCTGCGGC CTCCTAAGGT CCTGAGCCCT GGGGTGAGTC ATCGTTTGCG GGACCGGGAG 
TTGATTCTCT TTGCTGACTT GCCCTGCCAG TACCATCGCT TGGATGTCCC CCGGCCTTTT 
GTCTCCCGGG GCCCTCTAAC TGTAGAGGAG ACACCCAGGG TACAGGGAGG AACTCAACCC 
TCCAGGCTTC TGTTGGCTGA GGACTCAGAG GAAGAAGTAG ATTCCCTTTT GGACAAGTGT 
GTGGTGAAAG GACCAAGGAC CTCCTCTTCG GCAACAGTCG TTCCAGAGAG TGATGAAGAA 
GGGCCTTCCC CTGCCCCAGA TGGGCCCGGG CCACCTTCTG CCTTCAACTT GAACAGCGAC 
ACGGATGAGG AAGAAAGTCA GGAATCAGGA GCAGGGGAGG CCTCTTCAGC TGCCAGAAGA 
GGTACCGCTG CAGAGACGGA ACAGCCTGAA CCTGTCACAG CCGAGATCCA GATTGAGAAG 
GATCAGTGTT CCGTGAAGGA GAGGAACGGG GACACAGAAA TCGAGAGGGA TGTGAGGAAT 
GGGGTGGTTC CGACTGGAGT GATTCTGGAG AGGAGCCAGC CTTCTGGGGA GGACAGTGAC 
ACAGATGTGA GTGATGAGAG TGGGCCTCGA AGAAGGCTTG TTGGAGTCCG TCCGAAAAGG 
GCCTGGTCTT GTAACTTCAT AGACAGTGAT ACCGATGGAG AGGACGAGGG GATCCCTGCT 
ACCCCAGCAG TGGTTCCCAT GAAGGAGAGG CAGATCTTCC ACGAAGCTGG TACACAGAGC 
CCCCAGGCAC CTGGTGTGGC ACGTTGGCAG GAGAGCCCAG CTGATGGTGA TACAGATATA 
GAGGAGGGGG AGGCCCCCCC AGACAGAAGC CAAGCCTCCG TGGTGATCGA CAGCAATACA 
GACGATGAGG AAGAAGTCTC AGCAGCACTG ACACTGGCAT GTCTAAGAGA GAGCCAGGCT 
GGTAAGTGGA ACCGAGATCC AGATGCAGAA GAGACCAGGG CCCAACCCGT GGCCCTTCTG 
GAGCGAAGCC AGGCCTCTGC TGGGGGAGAC AGTGACACAG ATGTGGAGGA AGAGGGGCTC 
CCAGTGGAAA GGAGGGGAAT GGTTCCCAAG GGTTACATGG ACAGGGAATA TTCAAAAAAG 
AGCCAGCACC CTCCCAGGGA CAGTGATACA GAGGGGAAGG AAGATGAGAG CTCACCGGGG 
GTCCACCTGG AGAGAAGCCA GGCCTCTGCA CAAGTGGAGG ATGAGGTCCC ACTGGGGCCA 
GCTGTTGCAC TTCCGGAGAA GCGTCAGGTA CAAGGCATAG TGTGGCCACA TCACACCGAT 
GCGGAGGCAG AAGGGGACCC GGCACAGCTC CCCGTGCTGC GTCTAGAGGA AGCCCGGCCT 
CCTCTAGCTG GGGACTGTGA ACTGGATTCG GAGAACACAT CTGCTGTCAG AAAGAGCCAG 
CTTCCGGCGG AAAAAGATGC TGGGACCACG TGGGCTGCAG CCGTTCCTGA ACAGGACAGA 
GCACTTGCCA TCGGGACCCA GGGTGGGTCA TCCACGGCAC CAGGGGAGCA AGACCTTCTC 
CCGGTCTCAA GGGAAGACCT AGCAGATATG GTGGTGGACA TAGGCACTCC AGGGGAGCCC 
CAACCGCAGA GAGAGGGGGC CCAGACCACC ACAGGAAGGG AGAGAGAACC ATATGGGAAT 
AGGGCCACAG ACTCTGGAGA CAACCTCCAC GATTCTGAAG ATCTGGACCT ACCAGCTACC 
CAGTGCTTTG TAGACAGAAA GAATCAGAGC CTGGAAGCAG CCCCCAGCAT GGAGGATGAG 
CCCACCCAGG CCTTCCTGTT TCCTCTGCCC CAAGAGCCTG GCCCTTCCTG TTGCAGCTCC 
CAGGCCACAG GTTCCTTGGA TGAGTCATGG GAGGCCTTGG CGACACAGCC ATTCTGTCCG 
AGAGAATCTG AGGCCTCCGA GACCCAAACC GTTGTCACCC TCCTTGACAC CCATGCATCT 
TGGCCCTCTC CATCTAGGAC AGCACAGCAA GAGCAACATC CAGAGAGCCC AGTCCATGCA 
GAGCCACTGG GGATGGAAGG CAGAGAGATG CAGACTGTGG AGAAAGAAAT GGGTACCCCA 
AGAGAAACAG CAGAGAGGGT GATCCCTGAG GGAGGGCCAC CGCAGGAGGA AACCAAGAAA 
CTGCCCTCAG AAGGAGAGAG GGAAGATGTG ACGGGAGAGG AAGAATTAAT CGGGGGGATA 
CAGGGCAGAG AAAAAAATCA GGTGTTTGCT AGAGATACTC AGAGCCAAGA ATCTGACAAA 
AAAGTGAAAA GTGCAAGTAC TGGAAGGGGA ATGGAGATTT TAAAGGTAGA AATTGAGACA 
CCCAAGGAAA CACAAGAGAA AGAGAGAGAA AAGCAGACTC TCGCAGGGGA AATATTTGAG 
AGTGAAGCAG GGAAACTGGT AGTAGAGAGA GAGAGTGAGG CAGATGGGTT AGAAGTCAAG 
GAACCCCAAG AGCTACTGGA CAGAAGCCCA CAGATGGGGG AGACAGAGGC GGGGGACCAG 
GACCAGAAAG GCCAAGCCTC TGGTCTGCCA TCAGAGCCTG GAGCAGGGGC AGGAGACCTT 
CAGGGACTTG CTTCAGACCC AGTAGCTTCT GGGAGCCAGG CAGGTGGAGG AAGGGGAGTC 
CCAGGGAGCC CCAGGAGGCA GCAGAGAGGT GACTTGAATT GCGAGATGCC ACCTGCTCAG 
AAGGCTTCCA GGGGTGATCA GGAATCCCCA GATGCTTGTC TGCCTCCTGC AGCGCCTGAA 
GCCTCAGCCG CACTCCCAAA TTCCCTCATC ATCTCTCAGA TCCAAAAACA TCCCGCACCT 
CAGTCCCTCC TTTTTCCCTC TCTAGCTCCT TTAGAACTGC CTATTCCCAG GACCAGACAA 
AATGAGACTC AGGAAGCTCC AGAGACTCCC TTCTCCTCAG AGCTGAACTC TGTCCACCCA 
GAACCCAAAG TCAGGCTCCA GGCGTCCTCT CCAGTTTCTT CTCTACCCCT TGAGCCCCAC 
CCTACCACCC CCACAGGCCA GCCTATTGCC CTTGAACCCA TATCTGGGGT CTCTTGGAGC 
AGGACACGTA GTTCCTTTGA TGTAACTGCC TCATCAGTTG TCCCCACAGC CGTTGCACTG 
CAGCCATCCA TCTCCACAGA CAAGCCTGTC ACCCCTAAGC CCACACTTCG GGCCCCTCGG 
GGCAGGGCAC AGAGGTCTTC TGTCAAAACC CCTGAACCCA ATGTCACCAC AGACCAGCCT 
GTTGCCCCTG AGCTCACAGC TATGGCAACT CGGGGCAGGG CACAGAGGTC TTCTGTCAAG 
ATTCCCAAAC CAGATAACCC CACAACACCC AAGCCCCAGC CTTCCACTTC CACAGACCAG 
CCTGTCATCC CCAAACCCAC ATCTCGGGCC CCTCGGGGCA GGACACCTAG GTCTTCTGCC 
AAGACTCCTG AACCAGCTGT CCCCACAGCC TCTGAGCTCC AGCCTGCTGC CCCTAAAGAC 
CAGCCTGTTG CTCCTGAGCT CACATCTAGA GCCACTCGGG GCAGGACACA GAGGTCTATC 
AAGACTTCCA AACCAGATAT GCCCACAACT CCCGAGCCCC AGCCTTCCAC CTCCGCAGAC 
CAGCCTGTCA CCCCCAAACC CACGTCTCGG GCCCCTCGGG GCAGGACACC TAGGTCTTCC 
GCCAAGACTC CTGAGCCAGT TGTCTCCACA GCCTCTGAGC TCCAGCCTTC TGCCCTCACA 
GATGAGCCTG TCACTCCTGA GCTCACATCT AGGGCTACTC GGGGTCGGGC ACAGAGGTCC 
TCTGTCAAAA CCCCTGATCC AGTTACCACC ACAACACCTG AGCTCCAGCC TTCCACCTCC 
ACAGACCAGC TTGTCACCCC CAAACGCACA TCTCGGGCCC CTCGAGGCAG GACACGTAGG 
TTGTCTGCCA AGACTCCTGA ACCAGTTGTT CCCACAGCCT CTGAGCTCCA GCCTTCTGCC 
CCTGCAGACC AGCCTGTTGG TCCTTGGGCC ACTCAGTGTA GAAGACATAG GTCTTCTGTC 
AAGACCCCAG AACCAGTTGT CCCCACAGTC CCTGAACCTC AGCCTTCCAC TTCTAAAGAC 
CAGTCTGTCA CTCCTGAGCC CACATCTCAG GCCACTGAGA GCCAAACACA TAGGTCCTCT 
ATCAAGACAT CCCAGCCAAC GGAACCCACA GCCTCTGACC TCAAACCTTC CTCCCCCACA 
GACCAGCTTG TCACTCCCAA GGTCATAGCT CAGGGTGGTC CAAGCAGGAC AGGAAGGGCT 
TCCACAGCAA GTGCTGTGCT GGTTCCTACT ACCCCTGAAT TCCAGTCTCC AGTCCCGTCA 
GAACAGCCTC TTCCCCCTGA CCCCATCCCT GAAGTCAACT GCAGCAGGAG GCTGAGGGCC 
ACTAGGAAAC ATGGGTCTCC CACAGCTCAT GTTCATGAGC CCTGCACCGT ACCCCCTGAA 
CCTAACTCCC ACTCTTCAAG GAACCAAAGA CATGGGGCAG TGAAAGCAGC CAAGCCCCTT 
AGCACCATTC CTGAGCCTGC CTTTGCCCAG CTCCCCGAGG CACCGCCTCA CACTCCCCAG 
GTGCCAAAGG AGGAGGCAGC AGATGGATCA GGCTTCACCC CAGAGCCCCA GCCTAGGGCC 
TCTCAAAACC ACAAGAGGCC TTCAGCTACT GCACATTCAC CTCCACTTCA AAAACGGCTC 
CAGAGAGGGA GAGTCCCTCA GAAGGCAGCA TCCCTTAAGG AAGAAGAAAA TCCAGTAGCG 
AGGCCGAGGA AGGAAGAGGG TGTAGTGATT CCAGGTCCAG GCAAGAGAAA GAGAGAGCAG 
ACAGAGGAGG AGTCCCAGGG AAGACCGAGC CGCAGCCTGC GACGGACCAG ACCGATGCAG 
GAGTCCACGG CCCCCAAAGT GCTGTTCACC GGTGTGGTGG ATGCTCGCGG AGAGAGGACG 
GTGCTGGCCC TGGGGGGCAG TTTGGCCAGC TCGGTGGCCG AGGCTTCTCA CCTGGTGACT 
GATCGGATCC GCCGGACGGT CAAGTTTCTG TGTGCCCTGG GCCGGGGCAT CCCCATCCTC 
TCCCTGGCCT GGCTGCATGA GTCCCGCAAG GCAGGCTGCT TCTTGCCCCC GGACGAATAT 
TTGGTGACTG ATCCTGAGCA GGAGAAGAAC TTCGGCTTCA GCCTTCGGGA GGCCCTGAGC 
CGAGCTCAGG AGCGAAGGCT GCTGGAGGGC TATGAGATTC ATGTGACCCC CGGAGTCCAG 
CCACCGCCAC CTCAGATGGG AGAAATCATC AGCTGCTGTG GAGGCGCCAT CCTGCCCAGC 
ATGCCTCGGT CCTACAAGCC TCAGAGGTTC GTGATCACAT GTTCCCAGGA CTTTCCTCGA 
TGTGCTGTTC CATATCGGGT TGGGCTGCCT ATCCTCTCAC CCGAATTCCT GCTGACGGGA 
GTACTGAAGC AGGAAGTCAA GCCAGAGGCC TTTGCCTTCT CCACTGTGGA AATGTCATCC 
ACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012019796.1
CDS	160..5925
Translation	MEDTQILNWEVEEEEEVAESPNESLGYSLEPLAQLHIFSSSYGPEKDFPLYLGKNMIGRMPDCSVALPFSSISKQHAVIEISAWDKAPVLRDCGSLNGTQILRPPKVLSPGVSHRLRDRELILFADLPCQYHRLDVPRPFVSRGPLTVEETPRVQGGTQPSRLLLAEDSEEEVDSLLDKCVVKGPRTSSSATVVPESDEEGPSPAPDGPGPPSAFNLNSDTDEEESQESGAGEASSAARRGTAAETEQPEPVTAEIQIEKDQCSVKERNGDTEIERDVRNGVVPTGVILERSQPSGEDSDTDVSDESGPRRRLVGVRPKRAWSCNFIDSDTDGEDEGIPATPAVVPMKERQIFHEAGTQSPQAPGVARWQESPADGDTDIEEGEAPPDRSQASVVIDSNTDDEEEVSAALTLACLRESQAGKWNRDPDAEETRAQPVALLERSQASAGGDSDTDVEEEGLPVERRGMVPKGYMDREYSKKSQHPPRDSDTEGKEDESSPGVHLERSQASAQVEDEVPLGPAVALPEKRQVQGIVWPHHTDAEAEGDPAQLPVLRLEEARPPLAGDCELDSENTSAVRKSQLPAEKDAGTTWAAAVPEQDRALAIGTQGGSSTAPGEQDLLPVSREDLADMVVDIGTPGEPQPQREGAQTTTGREREPYGNRATDSGDNLHDSEDLDLPATQCFVDRKNQSLEAAPSMEDEPTQAFLFPLPQEPGPSCCSSQATGSLDESWEALATQPFCPRESEASETQTVVTLLDTHASWPSPSRTAQQEQHPESPVHAEPLGMEGREMQTVEKEMGTPRETAERVIPEGGPPQEETKKLPSEGEREDVTGEEELIGGIQGREKNQVFARDTQSQESDKKVKSASTGRGMEILKVEIETPKETQEKEREKQTLAGEIFESEAGKLVVERESEADGLEVKEPQELLDRSPQMGETEAGDQDQKGQASGLPSEPGAGAGDLQGLASDPVASGSQAGGGRGVPGSPRRQQRGDLNCEMPPAQKASRGDQESPDACLPPAAPEASAALPNSLIISQIQKHPAPQSLLFPSLAPLELPIPRTRQNETQEAPETPFSSELNSVHPEPKVRLQASSPVSSLPLEPHPTTPTGQPIALEPISGVSWSRTRSSFDVTASSVVPTAVALQPSISTDKPVTPKPTLRAPRGRAQRSSVKTPEPNVTTDQPVAPELTAMATRGRAQRSSVKIPKPDNPTTPKPQPSTSTDQPVIPKPTSRAPRGRTPRSSAKTPEPAVPTASELQPAAPKDQPVAPELTSRATRGRTQRSIKTSKPDMPTTPEPQPSTSADQPVTPKPTSRAPRGRTPRSSAKTPEPVVSTASELQPSALTDEPVTPELTSRATRGRAQRSSVKTPDPVTTTTPELQPSTSTDQLVTPKRTSRAPRGRTRRLSAKTPEPVVPTASELQPSAPADQPVGPWATQCRRHRSSVKTPEPVVPTVPEPQPSTSKDQSVTPEPTSQATESQTHRSSIKTSQPTEPTASDLKPSSPTDQLVTPKVIAQGGPSRTGRASTASAVLVPTTPEFQSPVPSEQPLPPDPIPEVNCSRRLRATRKHGSPTAHVHEPCTVPPEPNSHSSRNQRHGAVKAAKPLSTIPEPAFAQLPEAPPHTPQVPKEEAADGSGFTPEPQPRASQNHKRPSATAHSPPLQKRLQRGRVPQKAASLKEEENPVARPRKEEGVVIPGPGKRKREQTEEESQGRPSRSLRRTRPMQESTAPKVLFTGVVDARGERTVLALGGSLASSVAEASHLVTDRIRRTVKFLCALGRGIPILSLAWLHESRKAGCFLPPDEYLVTDPEQEKNFGFSLREALSRAQERRLLEGYEIHVTPGVQPPPPQMGEIISCCGGAILPSMPRSYKPQRFVITCSQDFPRCAVPYRVGLPILSPEFLLTGVLKQEVKPEAFAFSTVEMSST

Target ORF information:

RefSeq Version	XM_012164406.2
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon mediator of DNA damage checkpoint 1 (MDC1), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012164406.2

ORF Insert Sequence:

ATGGAAGACA CCCAGATTTT AAACTGGGAA GTTGAAGAAG AGGAGGAGGT TGCAGAGAGC 
CCCAATGAAT CTCTGGGGTA TAGCTTGGAG CCCCTAGCAC AGCTGCATAT CTTCAGTAGT 
TCCTATGGAC CAGAAAAAGA CTTCCCACTC TACCTTGGAA AGAACATGAT AGGCCGAATG 
CCTGATTGCT CTGTGGCCCT GCCCTTTTCA TCAATATCCA AACAACATGC AGTGATTGAA 
ATCTCTGCCT GGGACAAGGC GCCTGTCCTT CGGGATTGCG GGAGCCTCAA TGGCACTCAA 
ATCCTGCGGC CTCCTAAGGT CCTGAGCCCT GGGGTGAGTC ATCGTTTGCG GGACCGGGAG 
TTGATTCTCT TTGCTGACTT GCCCTGCCAG TACCATCGCT TGGATGTCCC CCGGCCTTTT 
GTCTCCCGGG GCCCTCTAAC TGTAGAGGAG ACACCCAGGG TACAGGGAGG AACTCAACCC 
TCCAGGCTTC TGTTGGCTGA GGACTCAGAG GAAGAAGTAG ATTCCCTTTT GGACAAGTGT 
GTGGTGAAAG GACCAAGGAC CTCCTCTTCG GCAACAGTCG TTCCAGAGAG TGATGAAGAA 
GGGCCTTCCC CTGCCCCAGA TGGGCCCGGG CCACCTTCTG CCTTCAACTT GAACAGCGAC 
ACGGATGAGG AAGAAAGTCA GGAATCAGGA GCAGGGGAGG CCTCTTCAGC TGCCAGAAGA 
GGTACCGCTG CAGAGACGGA ACAGCCTGAA CCTGTCACAG CCGAGATCCA GATTGAGAAG 
GATCAGTGTT CCGTGAAGGA GAGGAACGGG GACACAGAAA TCGAGAGGGA TGTGAGGAAT 
GGGGTGGTTC CGACTGGAGT GATTCTGGAG AGGAGCCAGC CTTCTGGGGA GGACAGTGAC 
ACAGATGTGA GTGATGAGAG TGGGCCTCGA AGAAGGCTTG TTGGAGTCCG TCCGAAAAGG 
GCCTGGTCTT GTAACTTCAT AGACAGTGAT ACCGATGGAG AGGACGAGGG GATCCCTGCT 
ACCCCAGCAG TGGTTCCCAT GAAGGAGAGG CAGATCTTCC ACGAAGCTGG TACACAGAGC 
CCCCAGGCAC CTGGTGTGGC ACGTTGGCAG GAGAGCCCAG CTGATGGTGA TACAGATATA 
GAGGAGGGGG AGGCCCCCCC AGACAGAAGC CAAGCCTCCG TGGTGATCGA CAGCAATACA 
GACGATGAGG AAGAAGTCTC AGCAGCACTG ACACTGGCAT GTCTAAGAGA GAGCCAGGCT 
GGTAAGTGGA ACCGAGATCC AGATGCAGAA GAGACCAGGG CCCAACCCGT GGCCCTTCTG 
GAGCGAAGCC AGGCCTCTGC TGGGGGAGAC AGTGACACAG ATGTGGAGGA AGAGGGGCTC 
CCAGTGGAAA GGAGGGGAAT GGTTCCCAAG GGTTACATGG ACAGGGAATA TTCAAAAAAG 
AGCCAGCACC CTCCCAGGGA CAGTGATACA GAGGGGAAGG AAGATGAGAG CTCACCGGGG 
GTCCACCTGG AGAGAAGCCA GGCCTCTGCA CAAGTGGAGG ATGAGGTCCC ACTGGGGCCA 
GCTGTTGCAC TTCCGGAGAA GCGTCAGGTA CAAGGCATAG TGTGGCCACA TCACACCGAT 
GCGGAGGCAG AAGGGGACCC GGCACAGCTC CCCGTGCTGC GTCTAGAGGA AGCCCGGCCT 
CCTCTAGCTG GGGACTGTGA ACTGGATTCG GAGAACACAT CTGCTGTCAG AAAGAGCCAG 
CTTCCGGCGG AAAAAGATGC TGGGACCACG TGGGCTGCAG CCGTTCCTGA ACAGGACAGA 
GCACTTGCCA TCGGGACCCA GGGTGGGTCA TCCACGGCAC CAGGGGAGCA AGACCTTCTC 
CCGGTCTCAA GGGAAGACCT AGCAGATATG GTGGTGGACA TAGGCACTCC AGGGGAGCCC 
CAACCGCAGA GAGAGGGGGC CCAGACCACC ACAGGAAGGG AGAGAGAACC ATATGGGAAT 
AGGGCCACAG ACTCTGGAGA CAACCTCCAC GATTCTGAAG ATCTGGACCT ACCAGCTACC 
CAGTGCTTTG TAGACAGAAA GAATCAGAGC CTGGAAGCAG CCCCCAGCAT GGAGGATGAG 
CCCACCCAGG CCTTCCTGTT TCCTCTGCCC CAAGAGCCTG GCCCTTCCTG TTGCAGCTCC 
CAGGCCACAG GTTCCTTGGA TGAGTCATGG GAGGCCTTGG CGACACAGCC ATTCTGTCCG 
AGAGAATCTG AGGCCTCCGA GACCCAAACC GTTGTCACCC TCCTTGACAC CCATGCATCT 
TGGCCCTCTC CATCTAGGAC AGCACAGCAA GAGCAACATC CAGAGAGCCC AGTCCATGCA 
GAGCCACTGG GGATGGAAGG CAGAGAGATG CAGACTGTGG AGAAAGAAAT GGGTACCCCA 
AGAGAAACAG CAGAGAGGGT GATCCCTGAG GGAGGGCCAC CGCAGGAGGA AACCAAGAAA 
CTGCCCTCAG AAGGAGAGAG GGAAGATGTG ACGGGAGAGG AAGAATTAAT CGGGGGGATA 
CAGGGCAGAG AAAAAAATCA GGTGTTTGCT AGAGATACTC AGAGCCAAGA ATCTGACAAA 
AAAGTGAAAA GTGCAAGTAC TGGAAGGGGA ATGGAGATTT TAAAGGTAGA AATTGAGACA 
CCCAAGGAAA CACAAGAGAA AGAGAGAGAA AAGCAGACTC TCGCAGGGGA AATATTTGAG 
AGTGAAGCAG GGAAACTGGT AGTAGAGAGA GAGAGTGAGG CAGATGGGTT AGAAGTCAAG 
GAACCCCAAG AGCTACTGGA CAGAAGCCCA CAGATGGGGG AGACAGAGGC GGGGGACCAG 
GACCAGAAAG GCCAAGCCTC TGGTCTGCCA TCAGAGCCTG GAGCAGGGGC AGGAGACCTT 
CAGGGACTTG CTTCAGACCC AGTAGCTTCT GGGAGCCAGG CAGGTGGAGG AAGGGGAGTC 
CCAGGGAGCC CCAGGAGGCA GCAGAGAGGT GACTTGAATT GCGAGATGCC ACCTGCTCAG 
AAGGCTTCCA GGGGTGATCA GGAATCCCCA GATGCTTGTC TGCCTCCTGC AGCGCCTGAA 
GCCTCAGCCG CACTCCCAAA TTCCCTCATC ATCTCTCAGA TCCAAAAACA TCCCGCACCT 
CAGTCCCTCC TTTTTCCCTC TCTAGCTCCT TTAGAACTGC CTATTCCCAG GACCAGACAA 
AATGAGACTC AGGAAGCTCC AGAGACTCCC TTCTCCTCAG AGCTGAACTC TGTCCACCCA 
GAACCCAAAG TCAGGCTCCA GGCGTCCTCT CCAGTTTCTT CTCTACCCCT TGAGCCCCAC 
CCTACCACCC CCACAGGCCA GCCTATTGCC CTTGAACCCA TATCTGGGGT CTCTTGGAGC 
AGGACACGTA GTTCCTTTGA TGTAACTGCC TCATCAGTTG TCCCCACAGC CGTTGCACTG 
CAGCCATCCA TCTCCACAGA CAAGCCTGTC ACCCCTAAGC CCACACTTCG GGCCCCTCGG 
GGCAGGGCAC AGAGGTCTTC TGTCAAAACC CCTGAACCCA ATGTCACCAC AGACCAGCCT 
GTTGCCCCTG AGCTCACAGC TATGGCAACT CGGGGCAGGG CACAGAGGTC TTCTGTCAAG 
ATTCCCAAAC CAGATAACCC CACAACACCC AAGCCCCAGC CTTCCACTTC CACAGACCAG 
CCTGTCATCC CCAAACCCAC ATCTCGGGCC CCTCGGGGCA GGACACCTAG GTCTTCTGCC 
AAGACTCCTG AACCAGCTGT CCCCACAGCC TCTGAGCTCC AGCCTGCTGC CCCTAAAGAC 
CAGCCTGTTG CTCCTGAGCT CACATCTAGA GCCACTCGGG GCAGGACACA GAGGTCTATC 
AAGACTTCCA AACCAGATAT GCCCACAACT CCCGAGCCCC AGCCTTCCAC CTCCGCAGAC 
CAGCCTGTCA CCCCCAAACC CACGTCTCGG GCCCCTCGGG GCAGGACACC TAGGTCTTCC 
GCCAAGACTC CTGAGCCAGT TGTCTCCACA GCCTCTGAGC TCCAGCCTTC TGCCCTCACA 
GATGAGCCTG TCACTCCTGA GCTCACATCT AGGGCTACTC GGGGTCGGGC ACAGAGGTCC 
TCTGTCAAAA CCCCTGATCC AGTTACCACC ACAACACCTG AGCTCCAGCC TTCCACCTCC 
ACAGACCAGC TTGTCACCCC CAAACGCACA TCTCGGGCCC CTCGAGGCAG GACACGTAGG 
TTGTCTGCCA AGACTCCTGA ACCAGTTGTT CCCACAGCCT CTGAGCTCCA GCCTTCTGCC 
CCTGCAGACC AGCCTGTTGG TCCTTGGGCC ACTCAGTGTA GAAGACATAG GTCTTCTGTC 
AAGACCCCAG AACCAGTTGT CCCCACAGTC CCTGAACCTC AGCCTTCCAC TTCTAAAGAC 
CAGTCTGTCA CTCCTGAGCC CACATCTCAG GCCACTGAGA GCCAAACACA TAGGTCCTCT 
ATCAAGACAT CCCAGCCAAC GGAACCCACA GCCTCTGACC TCAAACCTTC CTCCCCCACA 
GACCAGCTTG TCACTCCCAA GGTCATAGCT CAGGGTGGTC CAAGCAGGAC AGGAAGGGCT 
TCCACAGCAA GTGCTGTGCT GGTTCCTACT ACCCCTGAAT TCCAGTCTCC AGTCCCGTCA 
GAACAGCCTC TTCCCCCTGA CCCCATCCCT GAAGTCAACT GCAGCAGGAG GCTGAGGGCC 
ACTAGGAAAC ATGGGTCTCC CACAGCTCAT GTTCATGAGC CCTGCACCGT ACCCCCTGAA 
CCTAACTCCC ACTCTTCAAG GAACCAAAGA CATGGGGCAG TGAAAGCAGC CAAGCCCCTT 
AGCACCATTC CTGAGCCTGC CTTTGCCCAG CTCCCCGAGG CACCGCCTCA CACTCCCCAG 
GTGCCAAAGG AGGAGGCAGC AGATGGATCA GGCTTCACCC CAGAGCCCCA GCCTAGGGCC 
TCTCAAAACC ACAAGAGGCC TTCAGCTACT GCACATTCAC CTCCACTTCA AAAACGGCTC 
CAGAGAGGGA GAGTCCCTCA GAAGGCAGCA TCCCTTAAGG AAGAAGAAAA TCCAGTAGCG 
AGGCCGAGGA AGGAAGAGGG TGTAGTGATT CCAGGTCCAG GCAAGAGAAA GAGAGAGCAG 
ACAGAGGAGG AGTCCCAGGG AAGACCGAGC CGCAGCCTGC GACGGACCAG ACCGATGCAG 
GAGTCCACGG CCCCCAAAGT GCTGTTCACC GGTGTGGTGG ATGCTCGCGG AGAGAGGACG 
GTGCTGGCCC TGGGGGGCAG TTTGGCCAGC TCGGTGGCCG AGGCTTCTCA CCTGGTGACT 
GATCGGATCC GCCGGACGGT CAAGTTTCTG TGTGCCCTGG GCCGGGGCAT CCCCATCCTC 
TCCCTGGCCT GGCTGCATGA GTCCCGCAAG GCAGGCTGCT TCTTGCCCCC GGACGAATAT 
TTGGTGACTG ATCCTGAGCA GGAGAAGAAC TTCGGCTTCA GCCTTCGGGA GGCCCTGAGC 
CGAGCTCAGG AGCGAAGGCT GCTGGAGGGC TATGAGATTC ATGTGACCCC CGGAGTCCAG 
CCACCGCCAC CTCAGATGGG AGAAATCATC AGCTGCTGTG GAGGCGCCAT CCTGCCCAGC 
ATGCCTCGGT CCTACAAGCC TCAGAGGTTC GTGATCACAT GTTCCCAGGA CTTTCCTCGA 
TGTGCTGTTC CATATCGGGT TGGGCTGCCT ATCCTCTCAC CCGAATTCCT GCTGACGGGA 
GTACTGAAGC AGGAAGTCAA GCCAGAGGCC TTTGCCTTCT CCACTGTGGA AATGTCATCC 
ACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MDC1 ( XM_012164404.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_012019794.1 Ovis aries musimon mediator of DNA damage checkpoint 1 (MDC1), transcript variant X13, mRNA.

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CloneID	OOv35150
Clone ID Related Accession (Same CDS sequence)	XM_012164405.2 , XM_012164407.2 , XM_012164406.2 , XM_012164404.2
Accession Version	XM_012164404.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5766bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	mediator of DNA damage checkpoint protein 1 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011943095.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012164404.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGACA CCCAGATTTT AAACTGGGAA GTTGAAGAAG AGGAGGAGGT TGCAGAGAGC 
CCCAATGAAT CTCTGGGGTA TAGCTTGGAG CCCCTAGCAC AGCTGCATAT CTTCAGTAGT 
TCCTATGGAC CAGAAAAAGA CTTCCCACTC TACCTTGGAA AGAACATGAT AGGCCGAATG 
CCTGATTGCT CTGTGGCCCT GCCCTTTTCA TCAATATCCA AACAACATGC AGTGATTGAA 
ATCTCTGCCT GGGACAAGGC GCCTGTCCTT CGGGATTGCG GGAGCCTCAA TGGCACTCAA 
ATCCTGCGGC CTCCTAAGGT CCTGAGCCCT GGGGTGAGTC ATCGTTTGCG GGACCGGGAG 
TTGATTCTCT TTGCTGACTT GCCCTGCCAG TACCATCGCT TGGATGTCCC CCGGCCTTTT 
GTCTCCCGGG GCCCTCTAAC TGTAGAGGAG ACACCCAGGG TACAGGGAGG AACTCAACCC 
TCCAGGCTTC TGTTGGCTGA GGACTCAGAG GAAGAAGTAG ATTCCCTTTT GGACAAGTGT 
GTGGTGAAAG GACCAAGGAC CTCCTCTTCG GCAACAGTCG TTCCAGAGAG TGATGAAGAA 
GGGCCTTCCC CTGCCCCAGA TGGGCCCGGG CCACCTTCTG CCTTCAACTT GAACAGCGAC 
ACGGATGAGG AAGAAAGTCA GGAATCAGGA GCAGGGGAGG CCTCTTCAGC TGCCAGAAGA 
GGTACCGCTG CAGAGACGGA ACAGCCTGAA CCTGTCACAG CCGAGATCCA GATTGAGAAG 
GATCAGTGTT CCGTGAAGGA GAGGAACGGG GACACAGAAA TCGAGAGGGA TGTGAGGAAT 
GGGGTGGTTC CGACTGGAGT GATTCTGGAG AGGAGCCAGC CTTCTGGGGA GGACAGTGAC 
ACAGATGTGA GTGATGAGAG TGGGCCTCGA AGAAGGCTTG TTGGAGTCCG TCCGAAAAGG 
GCCTGGTCTT GTAACTTCAT AGACAGTGAT ACCGATGGAG AGGACGAGGG GATCCCTGCT 
ACCCCAGCAG TGGTTCCCAT GAAGGAGAGG CAGATCTTCC ACGAAGCTGG TACACAGAGC 
CCCCAGGCAC CTGGTGTGGC ACGTTGGCAG GAGAGCCCAG CTGATGGTGA TACAGATATA 
GAGGAGGGGG AGGCCCCCCC AGACAGAAGC CAAGCCTCCG TGGTGATCGA CAGCAATACA 
GACGATGAGG AAGAAGTCTC AGCAGCACTG ACACTGGCAT GTCTAAGAGA GAGCCAGGCT 
GGTAAGTGGA ACCGAGATCC AGATGCAGAA GAGACCAGGG CCCAACCCGT GGCCCTTCTG 
GAGCGAAGCC AGGCCTCTGC TGGGGGAGAC AGTGACACAG ATGTGGAGGA AGAGGGGCTC 
CCAGTGGAAA GGAGGGGAAT GGTTCCCAAG GGTTACATGG ACAGGGAATA TTCAAAAAAG 
AGCCAGCACC CTCCCAGGGA CAGTGATACA GAGGGGAAGG AAGATGAGAG CTCACCGGGG 
GTCCACCTGG AGAGAAGCCA GGCCTCTGCA CAAGTGGAGG ATGAGGTCCC ACTGGGGCCA 
GCTGTTGCAC TTCCGGAGAA GCGTCAGGTA CAAGGCATAG TGTGGCCACA TCACACCGAT 
GCGGAGGCAG AAGGGGACCC GGCACAGCTC CCCGTGCTGC GTCTAGAGGA AGCCCGGCCT 
CCTCTAGCTG GGGACTGTGA ACTGGATTCG GAGAACACAT CTGCTGTCAG AAAGAGCCAG 
CTTCCGGCGG AAAAAGATGC TGGGACCACG TGGGCTGCAG CCGTTCCTGA ACAGGACAGA 
GCACTTGCCA TCGGGACCCA GGGTGGGTCA TCCACGGCAC CAGGGGAGCA AGACCTTCTC 
CCGGTCTCAA GGGAAGACCT AGCAGATATG GTGGTGGACA TAGGCACTCC AGGGGAGCCC 
CAACCGCAGA GAGAGGGGGC CCAGACCACC ACAGGAAGGG AGAGAGAACC ATATGGGAAT 
AGGGCCACAG ACTCTGGAGA CAACCTCCAC GATTCTGAAG ATCTGGACCT ACCAGCTACC 
CAGTGCTTTG TAGACAGAAA GAATCAGAGC CTGGAAGCAG CCCCCAGCAT GGAGGATGAG 
CCCACCCAGG CCTTCCTGTT TCCTCTGCCC CAAGAGCCTG GCCCTTCCTG TTGCAGCTCC 
CAGGCCACAG GTTCCTTGGA TGAGTCATGG GAGGCCTTGG CGACACAGCC ATTCTGTCCG 
AGAGAATCTG AGGCCTCCGA GACCCAAACC GTTGTCACCC TCCTTGACAC CCATGCATCT 
TGGCCCTCTC CATCTAGGAC AGCACAGCAA GAGCAACATC CAGAGAGCCC AGTCCATGCA 
GAGCCACTGG GGATGGAAGG CAGAGAGATG CAGACTGTGG AGAAAGAAAT GGGTACCCCA 
AGAGAAACAG CAGAGAGGGT GATCCCTGAG GGAGGGCCAC CGCAGGAGGA AACCAAGAAA 
CTGCCCTCAG AAGGAGAGAG GGAAGATGTG ACGGGAGAGG AAGAATTAAT CGGGGGGATA 
CAGGGCAGAG AAAAAAATCA GGTGTTTGCT AGAGATACTC AGAGCCAAGA ATCTGACAAA 
AAAGTGAAAA GTGCAAGTAC TGGAAGGGGA ATGGAGATTT TAAAGGTAGA AATTGAGACA 
CCCAAGGAAA CACAAGAGAA AGAGAGAGAA AAGCAGACTC TCGCAGGGGA AATATTTGAG 
AGTGAAGCAG GGAAACTGGT AGTAGAGAGA GAGAGTGAGG CAGATGGGTT AGAAGTCAAG 
GAACCCCAAG AGCTACTGGA CAGAAGCCCA CAGATGGGGG AGACAGAGGC GGGGGACCAG 
GACCAGAAAG GCCAAGCCTC TGGTCTGCCA TCAGAGCCTG GAGCAGGGGC AGGAGACCTT 
CAGGGACTTG CTTCAGACCC AGTAGCTTCT GGGAGCCAGG CAGGTGGAGG AAGGGGAGTC 
CCAGGGAGCC CCAGGAGGCA GCAGAGAGGT GACTTGAATT GCGAGATGCC ACCTGCTCAG 
AAGGCTTCCA GGGGTGATCA GGAATCCCCA GATGCTTGTC TGCCTCCTGC AGCGCCTGAA 
GCCTCAGCCG CACTCCCAAA TTCCCTCATC ATCTCTCAGA TCCAAAAACA TCCCGCACCT 
CAGTCCCTCC TTTTTCCCTC TCTAGCTCCT TTAGAACTGC CTATTCCCAG GACCAGACAA 
AATGAGACTC AGGAAGCTCC AGAGACTCCC TTCTCCTCAG AGCTGAACTC TGTCCACCCA 
GAACCCAAAG TCAGGCTCCA GGCGTCCTCT CCAGTTTCTT CTCTACCCCT TGAGCCCCAC 
CCTACCACCC CCACAGGCCA GCCTATTGCC CTTGAACCCA TATCTGGGGT CTCTTGGAGC 
AGGACACGTA GTTCCTTTGA TGTAACTGCC TCATCAGTTG TCCCCACAGC CGTTGCACTG 
CAGCCATCCA TCTCCACAGA CAAGCCTGTC ACCCCTAAGC CCACACTTCG GGCCCCTCGG 
GGCAGGGCAC AGAGGTCTTC TGTCAAAACC CCTGAACCCA ATGTCACCAC AGACCAGCCT 
GTTGCCCCTG AGCTCACAGC TATGGCAACT CGGGGCAGGG CACAGAGGTC TTCTGTCAAG 
ATTCCCAAAC CAGATAACCC CACAACACCC AAGCCCCAGC CTTCCACTTC CACAGACCAG 
CCTGTCATCC CCAAACCCAC ATCTCGGGCC CCTCGGGGCA GGACACCTAG GTCTTCTGCC 
AAGACTCCTG AACCAGCTGT CCCCACAGCC TCTGAGCTCC AGCCTGCTGC CCCTAAAGAC 
CAGCCTGTTG CTCCTGAGCT CACATCTAGA GCCACTCGGG GCAGGACACA GAGGTCTATC 
AAGACTTCCA AACCAGATAT GCCCACAACT CCCGAGCCCC AGCCTTCCAC CTCCGCAGAC 
CAGCCTGTCA CCCCCAAACC CACGTCTCGG GCCCCTCGGG GCAGGACACC TAGGTCTTCC 
GCCAAGACTC CTGAGCCAGT TGTCTCCACA GCCTCTGAGC TCCAGCCTTC TGCCCTCACA 
GATGAGCCTG TCACTCCTGA GCTCACATCT AGGGCTACTC GGGGTCGGGC ACAGAGGTCC 
TCTGTCAAAA CCCCTGATCC AGTTACCACC ACAACACCTG AGCTCCAGCC TTCCACCTCC 
ACAGACCAGC TTGTCACCCC CAAACGCACA TCTCGGGCCC CTCGAGGCAG GACACGTAGG 
TTGTCTGCCA AGACTCCTGA ACCAGTTGTT CCCACAGCCT CTGAGCTCCA GCCTTCTGCC 
CCTGCAGACC AGCCTGTTGG TCCTTGGGCC ACTCAGTGTA GAAGACATAG GTCTTCTGTC 
AAGACCCCAG AACCAGTTGT CCCCACAGTC CCTGAACCTC AGCCTTCCAC TTCTAAAGAC 
CAGTCTGTCA CTCCTGAGCC CACATCTCAG GCCACTGAGA GCCAAACACA TAGGTCCTCT 
ATCAAGACAT CCCAGCCAAC GGAACCCACA GCCTCTGACC TCAAACCTTC CTCCCCCACA 
GACCAGCTTG TCACTCCCAA GGTCATAGCT CAGGGTGGTC CAAGCAGGAC AGGAAGGGCT 
TCCACAGCAA GTGCTGTGCT GGTTCCTACT ACCCCTGAAT TCCAGTCTCC AGTCCCGTCA 
GAACAGCCTC TTCCCCCTGA CCCCATCCCT GAAGTCAACT GCAGCAGGAG GCTGAGGGCC 
ACTAGGAAAC ATGGGTCTCC CACAGCTCAT GTTCATGAGC CCTGCACCGT ACCCCCTGAA 
CCTAACTCCC ACTCTTCAAG GAACCAAAGA CATGGGGCAG TGAAAGCAGC CAAGCCCCTT 
AGCACCATTC CTGAGCCTGC CTTTGCCCAG CTCCCCGAGG CACCGCCTCA CACTCCCCAG 
GTGCCAAAGG AGGAGGCAGC AGATGGATCA GGCTTCACCC CAGAGCCCCA GCCTAGGGCC 
TCTCAAAACC ACAAGAGGCC TTCAGCTACT GCACATTCAC CTCCACTTCA AAAACGGCTC 
CAGAGAGGGA GAGTCCCTCA GAAGGCAGCA TCCCTTAAGG AAGAAGAAAA TCCAGTAGCG 
AGGCCGAGGA AGGAAGAGGG TGTAGTGATT CCAGGTCCAG GCAAGAGAAA GAGAGAGCAG 
ACAGAGGAGG AGTCCCAGGG AAGACCGAGC CGCAGCCTGC GACGGACCAG ACCGATGCAG 
GAGTCCACGG CCCCCAAAGT GCTGTTCACC GGTGTGGTGG ATGCTCGCGG AGAGAGGACG 
GTGCTGGCCC TGGGGGGCAG TTTGGCCAGC TCGGTGGCCG AGGCTTCTCA CCTGGTGACT 
GATCGGATCC GCCGGACGGT CAAGTTTCTG TGTGCCCTGG GCCGGGGCAT CCCCATCCTC 
TCCCTGGCCT GGCTGCATGA GTCCCGCAAG GCAGGCTGCT TCTTGCCCCC GGACGAATAT 
TTGGTGACTG ATCCTGAGCA GGAGAAGAAC TTCGGCTTCA GCCTTCGGGA GGCCCTGAGC 
CGAGCTCAGG AGCGAAGGCT GCTGGAGGGC TATGAGATTC ATGTGACCCC CGGAGTCCAG 
CCACCGCCAC CTCAGATGGG AGAAATCATC AGCTGCTGTG GAGGCGCCAT CCTGCCCAGC 
ATGCCTCGGT CCTACAAGCC TCAGAGGTTC GTGATCACAT GTTCCCAGGA CTTTCCTCGA 
TGTGCTGTTC CATATCGGGT TGGGCTGCCT ATCCTCTCAC CCGAATTCCT GCTGACGGGA 
GTACTGAAGC AGGAAGTCAA GCCAGAGGCC TTTGCCTTCT CCACTGTGGA AATGTCATCC 
ACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012019794.1
CDS	193..5958
Translation	MEDTQILNWEVEEEEEVAESPNESLGYSLEPLAQLHIFSSSYGPEKDFPLYLGKNMIGRMPDCSVALPFSSISKQHAVIEISAWDKAPVLRDCGSLNGTQILRPPKVLSPGVSHRLRDRELILFADLPCQYHRLDVPRPFVSRGPLTVEETPRVQGGTQPSRLLLAEDSEEEVDSLLDKCVVKGPRTSSSATVVPESDEEGPSPAPDGPGPPSAFNLNSDTDEEESQESGAGEASSAARRGTAAETEQPEPVTAEIQIEKDQCSVKERNGDTEIERDVRNGVVPTGVILERSQPSGEDSDTDVSDESGPRRRLVGVRPKRAWSCNFIDSDTDGEDEGIPATPAVVPMKERQIFHEAGTQSPQAPGVARWQESPADGDTDIEEGEAPPDRSQASVVIDSNTDDEEEVSAALTLACLRESQAGKWNRDPDAEETRAQPVALLERSQASAGGDSDTDVEEEGLPVERRGMVPKGYMDREYSKKSQHPPRDSDTEGKEDESSPGVHLERSQASAQVEDEVPLGPAVALPEKRQVQGIVWPHHTDAEAEGDPAQLPVLRLEEARPPLAGDCELDSENTSAVRKSQLPAEKDAGTTWAAAVPEQDRALAIGTQGGSSTAPGEQDLLPVSREDLADMVVDIGTPGEPQPQREGAQTTTGREREPYGNRATDSGDNLHDSEDLDLPATQCFVDRKNQSLEAAPSMEDEPTQAFLFPLPQEPGPSCCSSQATGSLDESWEALATQPFCPRESEASETQTVVTLLDTHASWPSPSRTAQQEQHPESPVHAEPLGMEGREMQTVEKEMGTPRETAERVIPEGGPPQEETKKLPSEGEREDVTGEEELIGGIQGREKNQVFARDTQSQESDKKVKSASTGRGMEILKVEIETPKETQEKEREKQTLAGEIFESEAGKLVVERESEADGLEVKEPQELLDRSPQMGETEAGDQDQKGQASGLPSEPGAGAGDLQGLASDPVASGSQAGGGRGVPGSPRRQQRGDLNCEMPPAQKASRGDQESPDACLPPAAPEASAALPNSLIISQIQKHPAPQSLLFPSLAPLELPIPRTRQNETQEAPETPFSSELNSVHPEPKVRLQASSPVSSLPLEPHPTTPTGQPIALEPISGVSWSRTRSSFDVTASSVVPTAVALQPSISTDKPVTPKPTLRAPRGRAQRSSVKTPEPNVTTDQPVAPELTAMATRGRAQRSSVKIPKPDNPTTPKPQPSTSTDQPVIPKPTSRAPRGRTPRSSAKTPEPAVPTASELQPAAPKDQPVAPELTSRATRGRTQRSIKTSKPDMPTTPEPQPSTSADQPVTPKPTSRAPRGRTPRSSAKTPEPVVSTASELQPSALTDEPVTPELTSRATRGRAQRSSVKTPDPVTTTTPELQPSTSTDQLVTPKRTSRAPRGRTRRLSAKTPEPVVPTASELQPSAPADQPVGPWATQCRRHRSSVKTPEPVVPTVPEPQPSTSKDQSVTPEPTSQATESQTHRSSIKTSQPTEPTASDLKPSSPTDQLVTPKVIAQGGPSRTGRASTASAVLVPTTPEFQSPVPSEQPLPPDPIPEVNCSRRLRATRKHGSPTAHVHEPCTVPPEPNSHSSRNQRHGAVKAAKPLSTIPEPAFAQLPEAPPHTPQVPKEEAADGSGFTPEPQPRASQNHKRPSATAHSPPLQKRLQRGRVPQKAASLKEEENPVARPRKEEGVVIPGPGKRKREQTEEESQGRPSRSLRRTRPMQESTAPKVLFTGVVDARGERTVLALGGSLASSVAEASHLVTDRIRRTVKFLCALGRGIPILSLAWLHESRKAGCFLPPDEYLVTDPEQEKNFGFSLREALSRAQERRLLEGYEIHVTPGVQPPPPQMGEIISCCGGAILPSMPRSYKPQRFVITCSQDFPRCAVPYRVGLPILSPEFLLTGVLKQEVKPEAFAFSTVEMSST

Target ORF information:

RefSeq Version	XM_012164404.2
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon mediator of DNA damage checkpoint 1 (MDC1), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012164404.2

ORF Insert Sequence:

ATGGAAGACA CCCAGATTTT AAACTGGGAA GTTGAAGAAG AGGAGGAGGT TGCAGAGAGC 
CCCAATGAAT CTCTGGGGTA TAGCTTGGAG CCCCTAGCAC AGCTGCATAT CTTCAGTAGT 
TCCTATGGAC CAGAAAAAGA CTTCCCACTC TACCTTGGAA AGAACATGAT AGGCCGAATG 
CCTGATTGCT CTGTGGCCCT GCCCTTTTCA TCAATATCCA AACAACATGC AGTGATTGAA 
ATCTCTGCCT GGGACAAGGC GCCTGTCCTT CGGGATTGCG GGAGCCTCAA TGGCACTCAA 
ATCCTGCGGC CTCCTAAGGT CCTGAGCCCT GGGGTGAGTC ATCGTTTGCG GGACCGGGAG 
TTGATTCTCT TTGCTGACTT GCCCTGCCAG TACCATCGCT TGGATGTCCC CCGGCCTTTT 
GTCTCCCGGG GCCCTCTAAC TGTAGAGGAG ACACCCAGGG TACAGGGAGG AACTCAACCC 
TCCAGGCTTC TGTTGGCTGA GGACTCAGAG GAAGAAGTAG ATTCCCTTTT GGACAAGTGT 
GTGGTGAAAG GACCAAGGAC CTCCTCTTCG GCAACAGTCG TTCCAGAGAG TGATGAAGAA 
GGGCCTTCCC CTGCCCCAGA TGGGCCCGGG CCACCTTCTG CCTTCAACTT GAACAGCGAC 
ACGGATGAGG AAGAAAGTCA GGAATCAGGA GCAGGGGAGG CCTCTTCAGC TGCCAGAAGA 
GGTACCGCTG CAGAGACGGA ACAGCCTGAA CCTGTCACAG CCGAGATCCA GATTGAGAAG 
GATCAGTGTT CCGTGAAGGA GAGGAACGGG GACACAGAAA TCGAGAGGGA TGTGAGGAAT 
GGGGTGGTTC CGACTGGAGT GATTCTGGAG AGGAGCCAGC CTTCTGGGGA GGACAGTGAC 
ACAGATGTGA GTGATGAGAG TGGGCCTCGA AGAAGGCTTG TTGGAGTCCG TCCGAAAAGG 
GCCTGGTCTT GTAACTTCAT AGACAGTGAT ACCGATGGAG AGGACGAGGG GATCCCTGCT 
ACCCCAGCAG TGGTTCCCAT GAAGGAGAGG CAGATCTTCC ACGAAGCTGG TACACAGAGC 
CCCCAGGCAC CTGGTGTGGC ACGTTGGCAG GAGAGCCCAG CTGATGGTGA TACAGATATA 
GAGGAGGGGG AGGCCCCCCC AGACAGAAGC CAAGCCTCCG TGGTGATCGA CAGCAATACA 
GACGATGAGG AAGAAGTCTC AGCAGCACTG ACACTGGCAT GTCTAAGAGA GAGCCAGGCT 
GGTAAGTGGA ACCGAGATCC AGATGCAGAA GAGACCAGGG CCCAACCCGT GGCCCTTCTG 
GAGCGAAGCC AGGCCTCTGC TGGGGGAGAC AGTGACACAG ATGTGGAGGA AGAGGGGCTC 
CCAGTGGAAA GGAGGGGAAT GGTTCCCAAG GGTTACATGG ACAGGGAATA TTCAAAAAAG 
AGCCAGCACC CTCCCAGGGA CAGTGATACA GAGGGGAAGG AAGATGAGAG CTCACCGGGG 
GTCCACCTGG AGAGAAGCCA GGCCTCTGCA CAAGTGGAGG ATGAGGTCCC ACTGGGGCCA 
GCTGTTGCAC TTCCGGAGAA GCGTCAGGTA CAAGGCATAG TGTGGCCACA TCACACCGAT 
GCGGAGGCAG AAGGGGACCC GGCACAGCTC CCCGTGCTGC GTCTAGAGGA AGCCCGGCCT 
CCTCTAGCTG GGGACTGTGA ACTGGATTCG GAGAACACAT CTGCTGTCAG AAAGAGCCAG 
CTTCCGGCGG AAAAAGATGC TGGGACCACG TGGGCTGCAG CCGTTCCTGA ACAGGACAGA 
GCACTTGCCA TCGGGACCCA GGGTGGGTCA TCCACGGCAC CAGGGGAGCA AGACCTTCTC 
CCGGTCTCAA GGGAAGACCT AGCAGATATG GTGGTGGACA TAGGCACTCC AGGGGAGCCC 
CAACCGCAGA GAGAGGGGGC CCAGACCACC ACAGGAAGGG AGAGAGAACC ATATGGGAAT 
AGGGCCACAG ACTCTGGAGA CAACCTCCAC GATTCTGAAG ATCTGGACCT ACCAGCTACC 
CAGTGCTTTG TAGACAGAAA GAATCAGAGC CTGGAAGCAG CCCCCAGCAT GGAGGATGAG 
CCCACCCAGG CCTTCCTGTT TCCTCTGCCC CAAGAGCCTG GCCCTTCCTG TTGCAGCTCC 
CAGGCCACAG GTTCCTTGGA TGAGTCATGG GAGGCCTTGG CGACACAGCC ATTCTGTCCG 
AGAGAATCTG AGGCCTCCGA GACCCAAACC GTTGTCACCC TCCTTGACAC CCATGCATCT 
TGGCCCTCTC CATCTAGGAC AGCACAGCAA GAGCAACATC CAGAGAGCCC AGTCCATGCA 
GAGCCACTGG GGATGGAAGG CAGAGAGATG CAGACTGTGG AGAAAGAAAT GGGTACCCCA 
AGAGAAACAG CAGAGAGGGT GATCCCTGAG GGAGGGCCAC CGCAGGAGGA AACCAAGAAA 
CTGCCCTCAG AAGGAGAGAG GGAAGATGTG ACGGGAGAGG AAGAATTAAT CGGGGGGATA 
CAGGGCAGAG AAAAAAATCA GGTGTTTGCT AGAGATACTC AGAGCCAAGA ATCTGACAAA 
AAAGTGAAAA GTGCAAGTAC TGGAAGGGGA ATGGAGATTT TAAAGGTAGA AATTGAGACA 
CCCAAGGAAA CACAAGAGAA AGAGAGAGAA AAGCAGACTC TCGCAGGGGA AATATTTGAG 
AGTGAAGCAG GGAAACTGGT AGTAGAGAGA GAGAGTGAGG CAGATGGGTT AGAAGTCAAG 
GAACCCCAAG AGCTACTGGA CAGAAGCCCA CAGATGGGGG AGACAGAGGC GGGGGACCAG 
GACCAGAAAG GCCAAGCCTC TGGTCTGCCA TCAGAGCCTG GAGCAGGGGC AGGAGACCTT 
CAGGGACTTG CTTCAGACCC AGTAGCTTCT GGGAGCCAGG CAGGTGGAGG AAGGGGAGTC 
CCAGGGAGCC CCAGGAGGCA GCAGAGAGGT GACTTGAATT GCGAGATGCC ACCTGCTCAG 
AAGGCTTCCA GGGGTGATCA GGAATCCCCA GATGCTTGTC TGCCTCCTGC AGCGCCTGAA 
GCCTCAGCCG CACTCCCAAA TTCCCTCATC ATCTCTCAGA TCCAAAAACA TCCCGCACCT 
CAGTCCCTCC TTTTTCCCTC TCTAGCTCCT TTAGAACTGC CTATTCCCAG GACCAGACAA 
AATGAGACTC AGGAAGCTCC AGAGACTCCC TTCTCCTCAG AGCTGAACTC TGTCCACCCA 
GAACCCAAAG TCAGGCTCCA GGCGTCCTCT CCAGTTTCTT CTCTACCCCT TGAGCCCCAC 
CCTACCACCC CCACAGGCCA GCCTATTGCC CTTGAACCCA TATCTGGGGT CTCTTGGAGC 
AGGACACGTA GTTCCTTTGA TGTAACTGCC TCATCAGTTG TCCCCACAGC CGTTGCACTG 
CAGCCATCCA TCTCCACAGA CAAGCCTGTC ACCCCTAAGC CCACACTTCG GGCCCCTCGG 
GGCAGGGCAC AGAGGTCTTC TGTCAAAACC CCTGAACCCA ATGTCACCAC AGACCAGCCT 
GTTGCCCCTG AGCTCACAGC TATGGCAACT CGGGGCAGGG CACAGAGGTC TTCTGTCAAG 
ATTCCCAAAC CAGATAACCC CACAACACCC AAGCCCCAGC CTTCCACTTC CACAGACCAG 
CCTGTCATCC CCAAACCCAC ATCTCGGGCC CCTCGGGGCA GGACACCTAG GTCTTCTGCC 
AAGACTCCTG AACCAGCTGT CCCCACAGCC TCTGAGCTCC AGCCTGCTGC CCCTAAAGAC 
CAGCCTGTTG CTCCTGAGCT CACATCTAGA GCCACTCGGG GCAGGACACA GAGGTCTATC 
AAGACTTCCA AACCAGATAT GCCCACAACT CCCGAGCCCC AGCCTTCCAC CTCCGCAGAC 
CAGCCTGTCA CCCCCAAACC CACGTCTCGG GCCCCTCGGG GCAGGACACC TAGGTCTTCC 
GCCAAGACTC CTGAGCCAGT TGTCTCCACA GCCTCTGAGC TCCAGCCTTC TGCCCTCACA 
GATGAGCCTG TCACTCCTGA GCTCACATCT AGGGCTACTC GGGGTCGGGC ACAGAGGTCC 
TCTGTCAAAA CCCCTGATCC AGTTACCACC ACAACACCTG AGCTCCAGCC TTCCACCTCC 
ACAGACCAGC TTGTCACCCC CAAACGCACA TCTCGGGCCC CTCGAGGCAG GACACGTAGG 
TTGTCTGCCA AGACTCCTGA ACCAGTTGTT CCCACAGCCT CTGAGCTCCA GCCTTCTGCC 
CCTGCAGACC AGCCTGTTGG TCCTTGGGCC ACTCAGTGTA GAAGACATAG GTCTTCTGTC 
AAGACCCCAG AACCAGTTGT CCCCACAGTC CCTGAACCTC AGCCTTCCAC TTCTAAAGAC 
CAGTCTGTCA CTCCTGAGCC CACATCTCAG GCCACTGAGA GCCAAACACA TAGGTCCTCT 
ATCAAGACAT CCCAGCCAAC GGAACCCACA GCCTCTGACC TCAAACCTTC CTCCCCCACA 
GACCAGCTTG TCACTCCCAA GGTCATAGCT CAGGGTGGTC CAAGCAGGAC AGGAAGGGCT 
TCCACAGCAA GTGCTGTGCT GGTTCCTACT ACCCCTGAAT TCCAGTCTCC AGTCCCGTCA 
GAACAGCCTC TTCCCCCTGA CCCCATCCCT GAAGTCAACT GCAGCAGGAG GCTGAGGGCC 
ACTAGGAAAC ATGGGTCTCC CACAGCTCAT GTTCATGAGC CCTGCACCGT ACCCCCTGAA 
CCTAACTCCC ACTCTTCAAG GAACCAAAGA CATGGGGCAG TGAAAGCAGC CAAGCCCCTT 
AGCACCATTC CTGAGCCTGC CTTTGCCCAG CTCCCCGAGG CACCGCCTCA CACTCCCCAG 
GTGCCAAAGG AGGAGGCAGC AGATGGATCA GGCTTCACCC CAGAGCCCCA GCCTAGGGCC 
TCTCAAAACC ACAAGAGGCC TTCAGCTACT GCACATTCAC CTCCACTTCA AAAACGGCTC 
CAGAGAGGGA GAGTCCCTCA GAAGGCAGCA TCCCTTAAGG AAGAAGAAAA TCCAGTAGCG 
AGGCCGAGGA AGGAAGAGGG TGTAGTGATT CCAGGTCCAG GCAAGAGAAA GAGAGAGCAG 
ACAGAGGAGG AGTCCCAGGG AAGACCGAGC CGCAGCCTGC GACGGACCAG ACCGATGCAG 
GAGTCCACGG CCCCCAAAGT GCTGTTCACC GGTGTGGTGG ATGCTCGCGG AGAGAGGACG 
GTGCTGGCCC TGGGGGGCAG TTTGGCCAGC TCGGTGGCCG AGGCTTCTCA CCTGGTGACT 
GATCGGATCC GCCGGACGGT CAAGTTTCTG TGTGCCCTGG GCCGGGGCAT CCCCATCCTC 
TCCCTGGCCT GGCTGCATGA GTCCCGCAAG GCAGGCTGCT TCTTGCCCCC GGACGAATAT 
TTGGTGACTG ATCCTGAGCA GGAGAAGAAC TTCGGCTTCA GCCTTCGGGA GGCCCTGAGC 
CGAGCTCAGG AGCGAAGGCT GCTGGAGGGC TATGAGATTC ATGTGACCCC CGGAGTCCAG 
CCACCGCCAC CTCAGATGGG AGAAATCATC AGCTGCTGTG GAGGCGCCAT CCTGCCCAGC 
ATGCCTCGGT CCTACAAGCC TCAGAGGTTC GTGATCACAT GTTCCCAGGA CTTTCCTCGA 
TGTGCTGTTC CATATCGGGT TGGGCTGCCT ATCCTCTCAC CCGAATTCCT GCTGACGGGA 
GTACTGAAGC AGGAAGTCAA GCCAGAGGCC TTTGCCTTCT CCACTGTGGA AATGTCATCC 
ACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MDC1 ( XM_012164408.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_012019798.1 Ovis aries musimon mediator of DNA damage checkpoint 1 (MDC1), transcript variant X17, mRNA.

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CloneID	OOv35151
Clone ID Related Accession (Same CDS sequence)	XM_012164408.2
Accession Version	XM_012164408.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5718bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	mediator of DNA damage checkpoint protein 1 isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011943095.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012164408.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGTGT CCCACTTTCC TTGTCTACAT TTTCCCTTTA AGATCATGGA AGACACCCAG 
ATTTTAAACT GGGAAGTTGA AGAAGAGGAG GAGGTTGCAG AGAGCCCCAA TGAATCTCTG 
GGGTATAGCT TGGAGCCCCT AGCACAGCTG CATATCTTCA GTAGTTCCTA TGGACCAGAA 
AAAGACTTCC CACTCTACCT TGGAAAGAAC ATGATAGGCC GAATGCCTGA TTGCTCTGTG 
GCCCTGCCCT TTTCATCAAT ATCCAAACAA CATGCAGTGA TTGAAATCTC TGCCTGGGAC 
AAGGCGCCTG TCCTTCGGGA TTGCGGGAGC CTCAATGGCA CTCAAATCCT GCGGCCTCCT 
AAGGTCCTGA GCCCTGGGGT GAGTCATCGT TTGCGGGACC GGGAGTTGAT TCTCTTTGCT 
GACTTGCCCT GCCAGTACCA TCGCTTGGAT GTCCCCCGGC CTTTTGTCTC CCGGGGCCCT 
CTAACTGTAG AGGAGACACC CAGGGTACAG GGAGGAACTC AACCCTCCAG GCTTCTGTTG 
GCTGAGGACT CAGAGGAAGA AGTAGATTCC CTTTTGGACA AGTGTGTGGT GAAAGGACCA 
AGGACCTCCT CTTCGGCAAC AGTCGTTCCA GAGAGTGATG AAGAAGGGCC TTCCCCTGCC 
CCAGATGGGC CCGGGCCACC TTCTGCCTTC AACTTGAACA GCGACACGGA TGAGGAAGAA 
AGTCAGGAAT CAGGAGCAGG GGAGGCCTCT TCAGCTGCCA GAAGAGGTAC CGCTGCAGAG 
ACGGAACAGC CTGAACCTGT CACAGCCGAG ATCCAGATTG AGAAGGATCA GTGTTCCGTG 
AAGGAGAGGA ACGGGGACAC AGAAATCGAG AGGGATGTGA GGAATGGGGT GGTTCCGACT 
GGAGTGATTC TGGAGAGGAG CCAGCCTTCT GGGGAGGACA GTGACACAGA TGTGAGTGAT 
GAGAGTGGGC CTCGAAGAAG GCTTGTTGGA GTCCGTCCGA AAAGGGCCTG GTCTTGTAAC 
TTCATAGACA GTGATACCGA TGGAGAGGAC GAGGGGATCC CTGCTACCCC AGCAGTGGTT 
CCCATGAAGG AGAGGCAGAT CTTCCACGAA GCTGGTACAC AGAGCCCCCA GGCACCTGGT 
GTGGCACGTT GGCAGGAGAG CCCAGCTGAT GGTGATACAG ATATAGAGGA GGGGGAGGCC 
CCCCCAGACA GAAGCCAAGC CTCCGTGGTG ATCGACAGCA ATACAGACGA TGAGGAAGAA 
GTCTCAGCAG CACTGACACT GGCATGTCTA AGAGAGAGCC AGGCTGGTAA GTGGAACCGA 
GATCCAGATG CAGAAGAGAC CAGGGCCCAA CCCGTGGCCC TTCTGGAGCG AAGCCAGGCC 
TCTGCTGGGG GAGACAGTGA CACAGATGTG GAGGAAGAGG GGCTCCCAGT GGAAAGGAGG 
GGAATGGTTC CCAAGGGTTA CATGGACAGG GAATATTCAA AAAAGAGCCA GCACCCTCCC 
AGGGACAGTG ATACAGAGGG GAAGGAAGAT GAGAGCTCAC CGGGGGTCCA CCTGGAGAGA 
AGCCAGGCCT CTGCACAAGT GGAGGATGAG GTCCCACTGG GGCCAGCTGT TGCACTTCCG 
GAGAAGCGTC AGGTACAAGG CATAGTGTGG CCACATCACA CCGATGCGGA GGCAGAAGGG 
GACCCGGCAC AGCTCCCCGT GCTGCGTCTA GAGGAAGCCC GGCCTCCTCT AGCTGGGGAC 
TGTGAACTGG ATTCGGAGAA CACATCTGCT GTCAGAAAGA GCCAGCTTCC GGCGGAAAAA 
GATGCTGGGA CCACGTGGGC TGCAGCCGTT CCTGAACAGG ACAGAGCACT TGCCATCGGG 
ACCCAGGGTG GGTCATCCAC GGCACCAGGG GAGCAAGACC TTCTCCCGGT CTCAAGGGAA 
GACCTAGCAG ATATGGTGGT GGACATAGGC ACTCCAGGGG AGCCCCAACC GCAGAGAGAG 
GGGGCCCAGA CCACCACAGG AAGGGAGAGA GAACCATATG GGAATAGGGC CACAGACTCT 
GGAGACAACC TCCACGATTC TGAAGATCTG GACCTACCAG CTACCCAGTG CTTTGTAGAC 
AGAAAGAATC AGAGCCTGGA AGGTTCCTTG GATGAGTCAT GGGAGGCCTT GGCGACACAG 
CCATTCTGTC CGAGAGAATC TGAGGCCTCC GAGACCCAAA CCGTTGTCAC CCTCCTTGAC 
ACCCATGCAT CTTGGCCCTC TCCATCTAGG ACAGCACAGC AAGAGCAACA TCCAGAGAGC 
CCAGTCCATG CAGAGCCACT GGGGATGGAA GGCAGAGAGA TGCAGACTGT GGAGAAAGAA 
ATGGGTACCC CAAGAGAAAC AGCAGAGAGG GTGATCCCTG AGGGAGGGCC ACCGCAGGAG 
GAAACCAAGA AACTGCCCTC AGAAGGAGAG AGGGAAGATG TGACGGGAGA GGAAGAATTA 
ATCGGGGGGA TACAGGGCAG AGAAAAAAAT CAGGTGTTTG CTAGAGATAC TCAGAGCCAA 
GAATCTGACA AAAAAGTGAA AAGTGCAAGT ACTGGAAGGG GAATGGAGAT TTTAAAGGTA 
GAAATTGAGA CACCCAAGGA AACACAAGAG AAAGAGAGAG AAAAGCAGAC TCTCGCAGGG 
GAAATATTTG AGAGTGAAGC AGGGAAACTG GTAGTAGAGA GAGAGAGTGA GGCAGATGGG 
TTAGAAGTCA AGGAACCCCA AGAGCTACTG GACAGAAGCC CACAGATGGG GGAGACAGAG 
GCGGGGGACC AGGACCAGAA AGGCCAAGCC TCTGGTCTGC CATCAGAGCC TGGAGCAGGG 
GCAGGAGACC TTCAGGGACT TGCTTCAGAC CCAGTAGCTT CTGGGAGCCA GGCAGGTGGA 
GGAAGGGGAG TCCCAGGGAG CCCCAGGAGG CAGCAGAGAG GTGACTTGAA TTGCGAGATG 
CCACCTGCTC AGAAGGCTTC CAGGGGTGAT CAGGAATCCC CAGATGCTTG TCTGCCTCCT 
GCAGCGCCTG AAGCCTCAGC CGCACTCCCA AATTCCCTCA TCATCTCTCA GATCCAAAAA 
CATCCCGCAC CTCAGTCCCT CCTTTTTCCC TCTCTAGCTC CTTTAGAACT GCCTATTCCC 
AGGACCAGAC AAAATGAGAC TCAGGAAGCT CCAGAGACTC CCTTCTCCTC AGAGCTGAAC 
TCTGTCCACC CAGAACCCAA AGTCAGGCTC CAGGCGTCCT CTCCAGTTTC TTCTCTACCC 
CTTGAGCCCC ACCCTACCAC CCCCACAGGC CAGCCTATTG CCCTTGAACC CATATCTGGG 
GTCTCTTGGA GCAGGACACG TAGTTCCTTT GATGTAACTG CCTCATCAGT TGTCCCCACA 
GCCGTTGCAC TGCAGCCATC CATCTCCACA GACAAGCCTG TCACCCCTAA GCCCACACTT 
CGGGCCCCTC GGGGCAGGGC ACAGAGGTCT TCTGTCAAAA CCCCTGAACC CAATGTCACC 
ACAGACCAGC CTGTTGCCCC TGAGCTCACA GCTATGGCAA CTCGGGGCAG GGCACAGAGG 
TCTTCTGTCA AGATTCCCAA ACCAGATAAC CCCACAACAC CCAAGCCCCA GCCTTCCACT 
TCCACAGACC AGCCTGTCAT CCCCAAACCC ACATCTCGGG CCCCTCGGGG CAGGACACCT 
AGGTCTTCTG CCAAGACTCC TGAACCAGCT GTCCCCACAG CCTCTGAGCT CCAGCCTGCT 
GCCCCTAAAG ACCAGCCTGT TGCTCCTGAG CTCACATCTA GAGCCACTCG GGGCAGGACA 
CAGAGGTCTA TCAAGACTTC CAAACCAGAT ATGCCCACAA CTCCCGAGCC CCAGCCTTCC 
ACCTCCGCAG ACCAGCCTGT CACCCCCAAA CCCACGTCTC GGGCCCCTCG GGGCAGGACA 
CCTAGGTCTT CCGCCAAGAC TCCTGAGCCA GTTGTCTCCA CAGCCTCTGA GCTCCAGCCT 
TCTGCCCTCA CAGATGAGCC TGTCACTCCT GAGCTCACAT CTAGGGCTAC TCGGGGTCGG 
GCACAGAGGT CCTCTGTCAA AACCCCTGAT CCAGTTACCA CCACAACACC TGAGCTCCAG 
CCTTCCACCT CCACAGACCA GCTTGTCACC CCCAAACGCA CATCTCGGGC CCCTCGAGGC 
AGGACACGTA GGTTGTCTGC CAAGACTCCT GAACCAGTTG TTCCCACAGC CTCTGAGCTC 
CAGCCTTCTG CCCCTGCAGA CCAGCCTGTT GGTCCTTGGG CCACTCAGTG TAGAAGACAT 
AGGTCTTCTG TCAAGACCCC AGAACCAGTT GTCCCCACAG TCCCTGAACC TCAGCCTTCC 
ACTTCTAAAG ACCAGTCTGT CACTCCTGAG CCCACATCTC AGGCCACTGA GAGCCAAACA 
CATAGGTCCT CTATCAAGAC ATCCCAGCCA ACGGAACCCA CAGCCTCTGA CCTCAAACCT 
TCCTCCCCCA CAGACCAGCT TGTCACTCCC AAGGTCATAG CTCAGGGTGG TCCAAGCAGG 
ACAGGAAGGG CTTCCACAGC AAGTGCTGTG CTGGTTCCTA CTACCCCTGA ATTCCAGTCT 
CCAGTCCCGT CAGAACAGCC TCTTCCCCCT GACCCCATCC CTGAAGTCAA CTGCAGCAGG 
AGGCTGAGGG CCACTAGGAA ACATGGGTCT CCCACAGCTC ATGTTCATGA GCCCTGCACC 
GTACCCCCTG AACCTAACTC CCACTCTTCA AGGAACCAAA GACATGGGGC AGTGAAAGCA 
GCCAAGCCCC TTAGCACCAT TCCTGAGCCT GCCTTTGCCC AGCTCCCCGA GGCACCGCCT 
CACACTCCCC AGGTGCCAAA GGAGGAGGCA GCAGATGGAT CAGGCTTCAC CCCAGAGCCC 
CAGCCTAGGG CCTCTCAAAA CCACAAGAGG CCTTCAGCTA CTGCACATTC ACCTCCACTT 
CAAAAACGGC TCCAGAGAGG GAGAGTCCCT CAGAAGGCAG CATCCCTTAA GGAAGAAGAA 
AATCCAGTAG CGAGGCCGAG GAAGGAAGAG GGTGTAGTGA TTCCAGGTCC AGGCAAGAGA 
AAGAGAGAGC AGACAGAGGA GGAGTCCCAG GGAAGACCGA GCCGCAGCCT GCGACGGACC 
AGACCGATGC AGGAGTCCAC GGCCCCCAAA GTGCTGTTCA CCGGTGTGGT GGATGCTCGC 
GGAGAGAGGA CGGTGCTGGC CCTGGGGGGC AGTTTGGCCA GCTCGGTGGC CGAGGCTTCT 
CACCTGGTGA CTGATCGGAT CCGCCGGACG GTCAAGTTTC TGTGTGCCCT GGGCCGGGGC 
ATCCCCATCC TCTCCCTGGC CTGGCTGCAT GAGTCCCGCA AGGCAGGCTG CTTCTTGCCC 
CCGGACGAAT ATTTGGTGAC TGATCCTGAG CAGGAGAAGA ACTTCGGCTT CAGCCTTCGG 
GAGGCCCTGA GCCGAGCTCA GGAGCGAAGG CTGCTGGAGG GCTATGAGAT TCATGTGACC 
CCCGGAGTCC AGCCACCGCC ACCTCAGATG GGAGAAATCA TCAGCTGCTG TGGAGGCGCC 
ATCCTGCCCA GCATGCCTCG GTCCTACAAG CCTCAGAGGT TCGTGATCAC ATGTTCCCAG 
GACTTTCCTC GATGTGCTGT TCCATATCGG GTTGGGCTGC CTATCCTCTC ACCCGAATTC 
CTGCTGACGG GAGTACTGAA GCAGGAAGTC AAGCCAGAGG CCTTTGCCTT CTCCACTGTG 
GAAATGTCAT CCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012019798.1
CDS	328..6045
Translation	MEVSHFPCLHFPFKIMEDTQILNWEVEEEEEVAESPNESLGYSLEPLAQLHIFSSSYGPEKDFPLYLGKNMIGRMPDCSVALPFSSISKQHAVIEISAWDKAPVLRDCGSLNGTQILRPPKVLSPGVSHRLRDRELILFADLPCQYHRLDVPRPFVSRGPLTVEETPRVQGGTQPSRLLLAEDSEEEVDSLLDKCVVKGPRTSSSATVVPESDEEGPSPAPDGPGPPSAFNLNSDTDEEESQESGAGEASSAARRGTAAETEQPEPVTAEIQIEKDQCSVKERNGDTEIERDVRNGVVPTGVILERSQPSGEDSDTDVSDESGPRRRLVGVRPKRAWSCNFIDSDTDGEDEGIPATPAVVPMKERQIFHEAGTQSPQAPGVARWQESPADGDTDIEEGEAPPDRSQASVVIDSNTDDEEEVSAALTLACLRESQAGKWNRDPDAEETRAQPVALLERSQASAGGDSDTDVEEEGLPVERRGMVPKGYMDREYSKKSQHPPRDSDTEGKEDESSPGVHLERSQASAQVEDEVPLGPAVALPEKRQVQGIVWPHHTDAEAEGDPAQLPVLRLEEARPPLAGDCELDSENTSAVRKSQLPAEKDAGTTWAAAVPEQDRALAIGTQGGSSTAPGEQDLLPVSREDLADMVVDIGTPGEPQPQREGAQTTTGREREPYGNRATDSGDNLHDSEDLDLPATQCFVDRKNQSLEGSLDESWEALATQPFCPRESEASETQTVVTLLDTHASWPSPSRTAQQEQHPESPVHAEPLGMEGREMQTVEKEMGTPRETAERVIPEGGPPQEETKKLPSEGEREDVTGEEELIGGIQGREKNQVFARDTQSQESDKKVKSASTGRGMEILKVEIETPKETQEKEREKQTLAGEIFESEAGKLVVERESEADGLEVKEPQELLDRSPQMGETEAGDQDQKGQASGLPSEPGAGAGDLQGLASDPVASGSQAGGGRGVPGSPRRQQRGDLNCEMPPAQKASRGDQESPDACLPPAAPEASAALPNSLIISQIQKHPAPQSLLFPSLAPLELPIPRTRQNETQEAPETPFSSELNSVHPEPKVRLQASSPVSSLPLEPHPTTPTGQPIALEPISGVSWSRTRSSFDVTASSVVPTAVALQPSISTDKPVTPKPTLRAPRGRAQRSSVKTPEPNVTTDQPVAPELTAMATRGRAQRSSVKIPKPDNPTTPKPQPSTSTDQPVIPKPTSRAPRGRTPRSSAKTPEPAVPTASELQPAAPKDQPVAPELTSRATRGRTQRSIKTSKPDMPTTPEPQPSTSADQPVTPKPTSRAPRGRTPRSSAKTPEPVVSTASELQPSALTDEPVTPELTSRATRGRAQRSSVKTPDPVTTTTPELQPSTSTDQLVTPKRTSRAPRGRTRRLSAKTPEPVVPTASELQPSAPADQPVGPWATQCRRHRSSVKTPEPVVPTVPEPQPSTSKDQSVTPEPTSQATESQTHRSSIKTSQPTEPTASDLKPSSPTDQLVTPKVIAQGGPSRTGRASTASAVLVPTTPEFQSPVPSEQPLPPDPIPEVNCSRRLRATRKHGSPTAHVHEPCTVPPEPNSHSSRNQRHGAVKAAKPLSTIPEPAFAQLPEAPPHTPQVPKEEAADGSGFTPEPQPRASQNHKRPSATAHSPPLQKRLQRGRVPQKAASLKEEENPVARPRKEEGVVIPGPGKRKREQTEEESQGRPSRSLRRTRPMQESTAPKVLFTGVVDARGERTVLALGGSLASSVAEASHLVTDRIRRTVKFLCALGRGIPILSLAWLHESRKAGCFLPPDEYLVTDPEQEKNFGFSLREALSRAQERRLLEGYEIHVTPGVQPPPPQMGEIISCCGGAILPSMPRSYKPQRFVITCSQDFPRCAVPYRVGLPILSPEFLLTGVLKQEVKPEAFAFSTVEMSST

Target ORF information:

RefSeq Version	XM_012164408.2
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon mediator of DNA damage checkpoint 1 (MDC1), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012164408.2

ORF Insert Sequence:

ATGGAGGTGT CCCACTTTCC TTGTCTACAT TTTCCCTTTA AGATCATGGA AGACACCCAG 
ATTTTAAACT GGGAAGTTGA AGAAGAGGAG GAGGTTGCAG AGAGCCCCAA TGAATCTCTG 
GGGTATAGCT TGGAGCCCCT AGCACAGCTG CATATCTTCA GTAGTTCCTA TGGACCAGAA 
AAAGACTTCC CACTCTACCT TGGAAAGAAC ATGATAGGCC GAATGCCTGA TTGCTCTGTG 
GCCCTGCCCT TTTCATCAAT ATCCAAACAA CATGCAGTGA TTGAAATCTC TGCCTGGGAC 
AAGGCGCCTG TCCTTCGGGA TTGCGGGAGC CTCAATGGCA CTCAAATCCT GCGGCCTCCT 
AAGGTCCTGA GCCCTGGGGT GAGTCATCGT TTGCGGGACC GGGAGTTGAT TCTCTTTGCT 
GACTTGCCCT GCCAGTACCA TCGCTTGGAT GTCCCCCGGC CTTTTGTCTC CCGGGGCCCT 
CTAACTGTAG AGGAGACACC CAGGGTACAG GGAGGAACTC AACCCTCCAG GCTTCTGTTG 
GCTGAGGACT CAGAGGAAGA AGTAGATTCC CTTTTGGACA AGTGTGTGGT GAAAGGACCA 
AGGACCTCCT CTTCGGCAAC AGTCGTTCCA GAGAGTGATG AAGAAGGGCC TTCCCCTGCC 
CCAGATGGGC CCGGGCCACC TTCTGCCTTC AACTTGAACA GCGACACGGA TGAGGAAGAA 
AGTCAGGAAT CAGGAGCAGG GGAGGCCTCT TCAGCTGCCA GAAGAGGTAC CGCTGCAGAG 
ACGGAACAGC CTGAACCTGT CACAGCCGAG ATCCAGATTG AGAAGGATCA GTGTTCCGTG 
AAGGAGAGGA ACGGGGACAC AGAAATCGAG AGGGATGTGA GGAATGGGGT GGTTCCGACT 
GGAGTGATTC TGGAGAGGAG CCAGCCTTCT GGGGAGGACA GTGACACAGA TGTGAGTGAT 
GAGAGTGGGC CTCGAAGAAG GCTTGTTGGA GTCCGTCCGA AAAGGGCCTG GTCTTGTAAC 
TTCATAGACA GTGATACCGA TGGAGAGGAC GAGGGGATCC CTGCTACCCC AGCAGTGGTT 
CCCATGAAGG AGAGGCAGAT CTTCCACGAA GCTGGTACAC AGAGCCCCCA GGCACCTGGT 
GTGGCACGTT GGCAGGAGAG CCCAGCTGAT GGTGATACAG ATATAGAGGA GGGGGAGGCC 
CCCCCAGACA GAAGCCAAGC CTCCGTGGTG ATCGACAGCA ATACAGACGA TGAGGAAGAA 
GTCTCAGCAG CACTGACACT GGCATGTCTA AGAGAGAGCC AGGCTGGTAA GTGGAACCGA 
GATCCAGATG CAGAAGAGAC CAGGGCCCAA CCCGTGGCCC TTCTGGAGCG AAGCCAGGCC 
TCTGCTGGGG GAGACAGTGA CACAGATGTG GAGGAAGAGG GGCTCCCAGT GGAAAGGAGG 
GGAATGGTTC CCAAGGGTTA CATGGACAGG GAATATTCAA AAAAGAGCCA GCACCCTCCC 
AGGGACAGTG ATACAGAGGG GAAGGAAGAT GAGAGCTCAC CGGGGGTCCA CCTGGAGAGA 
AGCCAGGCCT CTGCACAAGT GGAGGATGAG GTCCCACTGG GGCCAGCTGT TGCACTTCCG 
GAGAAGCGTC AGGTACAAGG CATAGTGTGG CCACATCACA CCGATGCGGA GGCAGAAGGG 
GACCCGGCAC AGCTCCCCGT GCTGCGTCTA GAGGAAGCCC GGCCTCCTCT AGCTGGGGAC 
TGTGAACTGG ATTCGGAGAA CACATCTGCT GTCAGAAAGA GCCAGCTTCC GGCGGAAAAA 
GATGCTGGGA CCACGTGGGC TGCAGCCGTT CCTGAACAGG ACAGAGCACT TGCCATCGGG 
ACCCAGGGTG GGTCATCCAC GGCACCAGGG GAGCAAGACC TTCTCCCGGT CTCAAGGGAA 
GACCTAGCAG ATATGGTGGT GGACATAGGC ACTCCAGGGG AGCCCCAACC GCAGAGAGAG 
GGGGCCCAGA CCACCACAGG AAGGGAGAGA GAACCATATG GGAATAGGGC CACAGACTCT 
GGAGACAACC TCCACGATTC TGAAGATCTG GACCTACCAG CTACCCAGTG CTTTGTAGAC 
AGAAAGAATC AGAGCCTGGA AGGTTCCTTG GATGAGTCAT GGGAGGCCTT GGCGACACAG 
CCATTCTGTC CGAGAGAATC TGAGGCCTCC GAGACCCAAA CCGTTGTCAC CCTCCTTGAC 
ACCCATGCAT CTTGGCCCTC TCCATCTAGG ACAGCACAGC AAGAGCAACA TCCAGAGAGC 
CCAGTCCATG CAGAGCCACT GGGGATGGAA GGCAGAGAGA TGCAGACTGT GGAGAAAGAA 
ATGGGTACCC CAAGAGAAAC AGCAGAGAGG GTGATCCCTG AGGGAGGGCC ACCGCAGGAG 
GAAACCAAGA AACTGCCCTC AGAAGGAGAG AGGGAAGATG TGACGGGAGA GGAAGAATTA 
ATCGGGGGGA TACAGGGCAG AGAAAAAAAT CAGGTGTTTG CTAGAGATAC TCAGAGCCAA 
GAATCTGACA AAAAAGTGAA AAGTGCAAGT ACTGGAAGGG GAATGGAGAT TTTAAAGGTA 
GAAATTGAGA CACCCAAGGA AACACAAGAG AAAGAGAGAG AAAAGCAGAC TCTCGCAGGG 
GAAATATTTG AGAGTGAAGC AGGGAAACTG GTAGTAGAGA GAGAGAGTGA GGCAGATGGG 
TTAGAAGTCA AGGAACCCCA AGAGCTACTG GACAGAAGCC CACAGATGGG GGAGACAGAG 
GCGGGGGACC AGGACCAGAA AGGCCAAGCC TCTGGTCTGC CATCAGAGCC TGGAGCAGGG 
GCAGGAGACC TTCAGGGACT TGCTTCAGAC CCAGTAGCTT CTGGGAGCCA GGCAGGTGGA 
GGAAGGGGAG TCCCAGGGAG CCCCAGGAGG CAGCAGAGAG GTGACTTGAA TTGCGAGATG 
CCACCTGCTC AGAAGGCTTC CAGGGGTGAT CAGGAATCCC CAGATGCTTG TCTGCCTCCT 
GCAGCGCCTG AAGCCTCAGC CGCACTCCCA AATTCCCTCA TCATCTCTCA GATCCAAAAA 
CATCCCGCAC CTCAGTCCCT CCTTTTTCCC TCTCTAGCTC CTTTAGAACT GCCTATTCCC 
AGGACCAGAC AAAATGAGAC TCAGGAAGCT CCAGAGACTC CCTTCTCCTC AGAGCTGAAC 
TCTGTCCACC CAGAACCCAA AGTCAGGCTC CAGGCGTCCT CTCCAGTTTC TTCTCTACCC 
CTTGAGCCCC ACCCTACCAC CCCCACAGGC CAGCCTATTG CCCTTGAACC CATATCTGGG 
GTCTCTTGGA GCAGGACACG TAGTTCCTTT GATGTAACTG CCTCATCAGT TGTCCCCACA 
GCCGTTGCAC TGCAGCCATC CATCTCCACA GACAAGCCTG TCACCCCTAA GCCCACACTT 
CGGGCCCCTC GGGGCAGGGC ACAGAGGTCT TCTGTCAAAA CCCCTGAACC CAATGTCACC 
ACAGACCAGC CTGTTGCCCC TGAGCTCACA GCTATGGCAA CTCGGGGCAG GGCACAGAGG 
TCTTCTGTCA AGATTCCCAA ACCAGATAAC CCCACAACAC CCAAGCCCCA GCCTTCCACT 
TCCACAGACC AGCCTGTCAT CCCCAAACCC ACATCTCGGG CCCCTCGGGG CAGGACACCT 
AGGTCTTCTG CCAAGACTCC TGAACCAGCT GTCCCCACAG CCTCTGAGCT CCAGCCTGCT 
GCCCCTAAAG ACCAGCCTGT TGCTCCTGAG CTCACATCTA GAGCCACTCG GGGCAGGACA 
CAGAGGTCTA TCAAGACTTC CAAACCAGAT ATGCCCACAA CTCCCGAGCC CCAGCCTTCC 
ACCTCCGCAG ACCAGCCTGT CACCCCCAAA CCCACGTCTC GGGCCCCTCG GGGCAGGACA 
CCTAGGTCTT CCGCCAAGAC TCCTGAGCCA GTTGTCTCCA CAGCCTCTGA GCTCCAGCCT 
TCTGCCCTCA CAGATGAGCC TGTCACTCCT GAGCTCACAT CTAGGGCTAC TCGGGGTCGG 
GCACAGAGGT CCTCTGTCAA AACCCCTGAT CCAGTTACCA CCACAACACC TGAGCTCCAG 
CCTTCCACCT CCACAGACCA GCTTGTCACC CCCAAACGCA CATCTCGGGC CCCTCGAGGC 
AGGACACGTA GGTTGTCTGC CAAGACTCCT GAACCAGTTG TTCCCACAGC CTCTGAGCTC 
CAGCCTTCTG CCCCTGCAGA CCAGCCTGTT GGTCCTTGGG CCACTCAGTG TAGAAGACAT 
AGGTCTTCTG TCAAGACCCC AGAACCAGTT GTCCCCACAG TCCCTGAACC TCAGCCTTCC 
ACTTCTAAAG ACCAGTCTGT CACTCCTGAG CCCACATCTC AGGCCACTGA GAGCCAAACA 
CATAGGTCCT CTATCAAGAC ATCCCAGCCA ACGGAACCCA CAGCCTCTGA CCTCAAACCT 
TCCTCCCCCA CAGACCAGCT TGTCACTCCC AAGGTCATAG CTCAGGGTGG TCCAAGCAGG 
ACAGGAAGGG CTTCCACAGC AAGTGCTGTG CTGGTTCCTA CTACCCCTGA ATTCCAGTCT 
CCAGTCCCGT CAGAACAGCC TCTTCCCCCT GACCCCATCC CTGAAGTCAA CTGCAGCAGG 
AGGCTGAGGG CCACTAGGAA ACATGGGTCT CCCACAGCTC ATGTTCATGA GCCCTGCACC 
GTACCCCCTG AACCTAACTC CCACTCTTCA AGGAACCAAA GACATGGGGC AGTGAAAGCA 
GCCAAGCCCC TTAGCACCAT TCCTGAGCCT GCCTTTGCCC AGCTCCCCGA GGCACCGCCT 
CACACTCCCC AGGTGCCAAA GGAGGAGGCA GCAGATGGAT CAGGCTTCAC CCCAGAGCCC 
CAGCCTAGGG CCTCTCAAAA CCACAAGAGG CCTTCAGCTA CTGCACATTC ACCTCCACTT 
CAAAAACGGC TCCAGAGAGG GAGAGTCCCT CAGAAGGCAG CATCCCTTAA GGAAGAAGAA 
AATCCAGTAG CGAGGCCGAG GAAGGAAGAG GGTGTAGTGA TTCCAGGTCC AGGCAAGAGA 
AAGAGAGAGC AGACAGAGGA GGAGTCCCAG GGAAGACCGA GCCGCAGCCT GCGACGGACC 
AGACCGATGC AGGAGTCCAC GGCCCCCAAA GTGCTGTTCA CCGGTGTGGT GGATGCTCGC 
GGAGAGAGGA CGGTGCTGGC CCTGGGGGGC AGTTTGGCCA GCTCGGTGGC CGAGGCTTCT 
CACCTGGTGA CTGATCGGAT CCGCCGGACG GTCAAGTTTC TGTGTGCCCT GGGCCGGGGC 
ATCCCCATCC TCTCCCTGGC CTGGCTGCAT GAGTCCCGCA AGGCAGGCTG CTTCTTGCCC 
CCGGACGAAT ATTTGGTGAC TGATCCTGAG CAGGAGAAGA ACTTCGGCTT CAGCCTTCGG 
GAGGCCCTGA GCCGAGCTCA GGAGCGAAGG CTGCTGGAGG GCTATGAGAT TCATGTGACC 
CCCGGAGTCC AGCCACCGCC ACCTCAGATG GGAGAAATCA TCAGCTGCTG TGGAGGCGCC 
ATCCTGCCCA GCATGCCTCG GTCCTACAAG CCTCAGAGGT TCGTGATCAC ATGTTCCCAG 
GACTTTCCTC GATGTGCTGT TCCATATCGG GTTGGGCTGC CTATCCTCTC ACCCGAATTC 
CTGCTGACGG GAGTACTGAA GCAGGAAGTC AAGCCAGAGG CCTTTGCCTT CTCCACTGTG 
GAAATGTCAT CCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MDC1 ( XM_012164409.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_012019799.1 Ovis aries musimon mediator of DNA damage checkpoint 1 (MDC1), transcript variant X18, mRNA.

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CloneID	OOv35152
Clone ID Related Accession (Same CDS sequence)	XM_012164409.2
Accession Version	XM_012164409.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5235bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	mediator of DNA damage checkpoint protein 1 isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011943095.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012164409.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGTGT CCCACTTTCC TTGTCTACAT TTTCCCTTTA AGATCATGGA AGACACCCAG 
ATTTTAAACT GGGAAGTTGA AGAAGAGGAG GAGGTTGCAG AGAGCCCCAA TGAATCTCTG 
GGGTATAGCT TGGAGCCCCT AGCACAGCTG CATATCTTCA GTAGTTCCTA TGGACCAGAA 
AAAGACTTCC CACTCTACCT TGGAAAGAAC ATGATAGGCC GAATGCCTGA TTGCTCTGTG 
GCCCTGCCCT TTTCATCAAT ATCCAAACAA CATGCAGTGA TTGAAATCTC TGCCTGGGAC 
AAGGCGCCTG TCCTTCGGGA TTGCGGGAGC CTCAATGGCA CTCAAATCCT GCGGCCTCCT 
AAGGTCCTGA GCCCTGGGGT GAGTCATCGT TTGCGGGACC GGGAGTTGAT TCTCTTTGCT 
GACTTGCCCT GCCAGTACCA TCGCTTGGAT GTCCCCCGGC CTTTTGTCTC CCGGGGCCCT 
CTAACTGTAG AGGAGACACC CAGGGTACAG GGAGGAACTC AACCCTCCAG GCTTCTGTTG 
GCTGAGGACT CAGAGGAAGA AGTAGATTCC CTTTTGGACA AGTGTGTGGT GAAAGGACCA 
AGGACCTCCT CTTCGGCAAC AGTCGTTCCA GAGAGTGATG AAGAAGGGCC TTCCCCTGCC 
CCAGATGGGC CCGGGCCACC TTCTGCCTTC AACTTGAACA GCGACACGGA TGAGGAAGAA 
AGTCAGGAAT CAGGAGCAGG GGAGGCCTCT TCAGCTGCCA GAAGAGGTAC CGCTGCAGAG 
ACGGAACAGC CTGAACCTGT CACAGCCGAG ATCCAGATTG AGAAGGATCA GTGTTCCGTG 
AAGGAGAGGA ACGGGGACAC AGAAATCGAG AGGGATGTGA GGAATGGGGT GGTTCCGACT 
GGAGTGATTC TGGAGAGGAG CCAGCCTTCT GGGGAGGACA GTGACACAGA TGTGAGTGAT 
GAGAGTGGGC CTCGAAGAAG GCTTGTTGGA GTCCGTCCGA AAAGGGCCTG GTCTTGTAAC 
TTCATAGACA GTGATACCGA TGGAGAGGAC GAGGGGATCC CTGCTACCCC AGCAGTGGTT 
CCCATGAAGG AGAGGCAGAT CTTCCACGAA GCTGGTACAC AGAGCCCCCA GGCACCTGGT 
GTGGCACGTT GGCAGGAGAG CCCAGCTGAT GGTGATACAG ATATAGAGGA GGGGGAGGCC 
CCCCCAGACA GAAGCCAAGC CTCCGTGGTG ATCGACAGCA ATACAGACGA TGAGGAAGAA 
GTCTCAGCAG CACTGACACT GGCATGTCTA AGAGAGAGCC AGGCTGGTAA GTGGAACCGA 
GATCCAGATG CAGAAGAGAC CAGGGCCCAA CCCGTGGCCC TTCTGGAGCG AAGCCAGGCC 
TCTGCTGGGG GAGACAGTGA CACAGATGTG GAGGAAGAGG GGCTCCCAGT GGAAAGGAGG 
GGAATGGTTC CCAAGGGTTA CATGGACAGG GAATATTCAA AAAAGAGCCA GCACCCTCCC 
AGGGACAGTG ATACAGAGGG GAAGGAAGAT GAGAGCTCAC CGGGGGTCCA CCTGGAGAGA 
AGCCAGGCCT CTGCACAAGT GGAGGATGAG GTCCCACTGG GGCCAGCTGT TGCACTTCCG 
GAGAAGCGTC AGGTACAAGG CATAGTGTGG CCACATCACA CCGATGCGGA GGCAGAAGGG 
GACCCGGCAC AGCTCCCCGT GCTGCGTCTA GAGGAAGCCC GGCCTCCTCT AGCTGGGGAC 
TGTGAACTGG ATTCGGAGAA CACATCTGCT GTCAGAAAGA GCCAGCTTCC GGCGGAAAAA 
GATGCTGGGA CCACGTGGGC TGCAGCCGTT CCTGAACAGG ACAGAGCACT TGCCATCGGG 
ACCCAGGGTG GGTCATCCAC GGCACCAGGG GAGCAAGACC TTCTCCCGGT CTCAAGGGAA 
GACCTAGCAG ATATGGTGGT GGACATAGGC ACTCCAGGGG AGCCCCAACC GCAGAGAGAG 
GGGGCCCAGA CCACCACAGG AAGGGAGAGA GAACCATATG GGAATAGGGC CACAGACTCT 
GGAGACAACC TCCACGATTC TGAAGATCTG GACCTACCAG CTACCCAGTG CTTTGTAGAC 
AGAAAGAATC AGAGCCTGGA AGCAGCCCCC AGCATGGAGG ATGAGCCCAC CCAGGCCTTC 
CTGTTTCCTC TGCCCCAAGA GCCTGGCCCT TCCTGTTGCA GCTCCCAGGC CACAGGTTCC 
TTGGATGAGT CATGGGAGGC CTTGGCGACA CAGCCATTCT GTCCGAGAGA ATCTGAGGCC 
TCCGAGACCC AAACCGTTGT CACCCTCCTT GACACCCATG CATCTTGGCC CTCTCCATCT 
AGGACAGCAC AGCAAGAGCA ACATCCAGAG AGCCCAGTCC ATGCAGAGCC ACTGGGGATG 
GAAGGCAGAG AGATGCAGAC TGTGGAGAAA GAAATGGGTG ACTTGAATTG CGAGATGCCA 
CCTGCTCAGA AGGCTTCCAG GGGTGATCAG GAATCCCCAG ATGCTTGTCT GCCTCCTGCA 
GCGCCTGAAG CCTCAGCCGC ACTCCCAAAT TCCCTCATCA TCTCTCAGAT CCAAAAACAT 
CCCGCACCTC AGTCCCTCCT TTTTCCCTCT CTAGCTCCTT TAGAACTGCC TATTCCCAGG 
ACCAGACAAA ATGAGACTCA GGAAGCTCCA GAGACTCCCT TCTCCTCAGA GCTGAACTCT 
GTCCACCCAG AACCCAAAGT CAGGCTCCAG GCGTCCTCTC CAGTTTCTTC TCTACCCCTT 
GAGCCCCACC CTACCACCCC CACAGGCCAG CCTATTGCCC TTGAACCCAT ATCTGGGGTC 
TCTTGGAGCA GGACACGTAG TTCCTTTGAT GTAACTGCCT CATCAGTTGT CCCCACAGCC 
GTTGCACTGC AGCCATCCAT CTCCACAGAC AAGCCTGTCA CCCCTAAGCC CACACTTCGG 
GCCCCTCGGG GCAGGGCACA GAGGTCTTCT GTCAAAACCC CTGAACCCAA TGTCACCACA 
GACCAGCCTG TTGCCCCTGA GCTCACAGCT ATGGCAACTC GGGGCAGGGC ACAGAGGTCT 
TCTGTCAAGA TTCCCAAACC AGATAACCCC ACAACACCCA AGCCCCAGCC TTCCACTTCC 
ACAGACCAGC CTGTCATCCC CAAACCCACA TCTCGGGCCC CTCGGGGCAG GACACCTAGG 
TCTTCTGCCA AGACTCCTGA ACCAGCTGTC CCCACAGCCT CTGAGCTCCA GCCTGCTGCC 
CCTAAAGACC AGCCTGTTGC TCCTGAGCTC ACATCTAGAG CCACTCGGGG CAGGACACAG 
AGGTCTATCA AGACTTCCAA ACCAGATATG CCCACAACTC CCGAGCCCCA GCCTTCCACC 
TCCGCAGACC AGCCTGTCAC CCCCAAACCC ACGTCTCGGG CCCCTCGGGG CAGGACACCT 
AGGTCTTCCG CCAAGACTCC TGAGCCAGTT GTCTCCACAG CCTCTGAGCT CCAGCCTTCT 
GCCCTCACAG ATGAGCCTGT CACTCCTGAG CTCACATCTA GGGCTACTCG GGGTCGGGCA 
CAGAGGTCCT CTGTCAAAAC CCCTGATCCA GTTACCACCA CAACACCTGA GCTCCAGCCT 
TCCACCTCCA CAGACCAGCT TGTCACCCCC AAACGCACAT CTCGGGCCCC TCGAGGCAGG 
ACACGTAGGT TGTCTGCCAA GACTCCTGAA CCAGTTGTTC CCACAGCCTC TGAGCTCCAG 
CCTTCTGCCC CTGCAGACCA GCCTGTTGGT CCTTGGGCCA CTCAGTGTAG AAGACATAGG 
TCTTCTGTCA AGACCCCAGA ACCAGTTGTC CCCACAGTCC CTGAACCTCA GCCTTCCACT 
TCTAAAGACC AGTCTGTCAC TCCTGAGCCC ACATCTCAGG CCACTGAGAG CCAAACACAT 
AGGTCCTCTA TCAAGACATC CCAGCCAACG GAACCCACAG CCTCTGACCT CAAACCTTCC 
TCCCCCACAG ACCAGCTTGT CACTCCCAAG GTCATAGCTC AGGGTGGTCC AAGCAGGACA 
GGAAGGGCTT CCACAGCAAG TGCTGTGCTG GTTCCTACTA CCCCTGAATT CCAGTCTCCA 
GTCCCGTCAG AACAGCCTCT TCCCCCTGAC CCCATCCCTG AAGTCAACTG CAGCAGGAGG 
CTGAGGGCCA CTAGGAAACA TGGGTCTCCC ACAGCTCATG TTCATGAGCC CTGCACCGTA 
CCCCCTGAAC CTAACTCCCA CTCTTCAAGG AACCAAAGAC ATGGGGCAGT GAAAGCAGCC 
AAGCCCCTTA GCACCATTCC TGAGCCTGCC TTTGCCCAGC TCCCCGAGGC ACCGCCTCAC 
ACTCCCCAGG TGCCAAAGGA GGAGGCAGCA GATGGATCAG GCTTCACCCC AGAGCCCCAG 
CCTAGGGCCT CTCAAAACCA CAAGAGGCCT TCAGCTACTG CACATTCACC TCCACTTCAA 
AAACGGCTCC AGAGAGGGAG AGTCCCTCAG AAGGCAGCAT CCCTTAAGGA AGAAGAAAAT 
CCAGTAGCGA GGCCGAGGAA GGAAGAGGGT GTAGTGATTC CAGGTCCAGG CAAGAGAAAG 
AGAGAGCAGA CAGAGGAGGA GTCCCAGGGA AGACCGAGCC GCAGCCTGCG ACGGACCAGA 
CCGATGCAGG AGTCCACGGC CCCCAAAGTG CTGTTCACCG GTGTGGTGGA TGCTCGCGGA 
GAGAGGACGG TGCTGGCCCT GGGGGGCAGT TTGGCCAGCT CGGTGGCCGA GGCTTCTCAC 
CTGGTGACTG ATCGGATCCG CCGGACGGTC AAGTTTCTGT GTGCCCTGGG CCGGGGCATC 
CCCATCCTCT CCCTGGCCTG GCTGCATGAG TCCCGCAAGG CAGGCTGCTT CTTGCCCCCG 
GACGAATATT TGGTGACTGA TCCTGAGCAG GAGAAGAACT TCGGCTTCAG CCTTCGGGAG 
GCCCTGAGCC GAGCTCAGGA GCGAAGGCTG CTGGAGGGCT ATGAGATTCA TGTGACCCCC 
GGAGTCCAGC CACCGCCACC TCAGATGGGA GAAATCATCA GCTGCTGTGG AGGCGCCATC 
CTGCCCAGCA TGCCTCGGTC CTACAAGCCT CAGAGGTTCG TGATCACATG TTCCCAGGAC 
TTTCCTCGAT GTGCTGTTCC ATATCGGGTT GGGCTGCCTA TCCTCTCACC CGAATTCCTG 
CTGACGGGAG TACTGAAGCA GGAAGTCAAG CCAGAGGCCT TTGCCTTCTC CACTGTGGAA 
ATGTCATCCA CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012019799.1
CDS	328..5562
Translation	MEVSHFPCLHFPFKIMEDTQILNWEVEEEEEVAESPNESLGYSLEPLAQLHIFSSSYGPEKDFPLYLGKNMIGRMPDCSVALPFSSISKQHAVIEISAWDKAPVLRDCGSLNGTQILRPPKVLSPGVSHRLRDRELILFADLPCQYHRLDVPRPFVSRGPLTVEETPRVQGGTQPSRLLLAEDSEEEVDSLLDKCVVKGPRTSSSATVVPESDEEGPSPAPDGPGPPSAFNLNSDTDEEESQESGAGEASSAARRGTAAETEQPEPVTAEIQIEKDQCSVKERNGDTEIERDVRNGVVPTGVILERSQPSGEDSDTDVSDESGPRRRLVGVRPKRAWSCNFIDSDTDGEDEGIPATPAVVPMKERQIFHEAGTQSPQAPGVARWQESPADGDTDIEEGEAPPDRSQASVVIDSNTDDEEEVSAALTLACLRESQAGKWNRDPDAEETRAQPVALLERSQASAGGDSDTDVEEEGLPVERRGMVPKGYMDREYSKKSQHPPRDSDTEGKEDESSPGVHLERSQASAQVEDEVPLGPAVALPEKRQVQGIVWPHHTDAEAEGDPAQLPVLRLEEARPPLAGDCELDSENTSAVRKSQLPAEKDAGTTWAAAVPEQDRALAIGTQGGSSTAPGEQDLLPVSREDLADMVVDIGTPGEPQPQREGAQTTTGREREPYGNRATDSGDNLHDSEDLDLPATQCFVDRKNQSLEAAPSMEDEPTQAFLFPLPQEPGPSCCSSQATGSLDESWEALATQPFCPRESEASETQTVVTLLDTHASWPSPSRTAQQEQHPESPVHAEPLGMEGREMQTVEKEMGDLNCEMPPAQKASRGDQESPDACLPPAAPEASAALPNSLIISQIQKHPAPQSLLFPSLAPLELPIPRTRQNETQEAPETPFSSELNSVHPEPKVRLQASSPVSSLPLEPHPTTPTGQPIALEPISGVSWSRTRSSFDVTASSVVPTAVALQPSISTDKPVTPKPTLRAPRGRAQRSSVKTPEPNVTTDQPVAPELTAMATRGRAQRSSVKIPKPDNPTTPKPQPSTSTDQPVIPKPTSRAPRGRTPRSSAKTPEPAVPTASELQPAAPKDQPVAPELTSRATRGRTQRSIKTSKPDMPTTPEPQPSTSADQPVTPKPTSRAPRGRTPRSSAKTPEPVVSTASELQPSALTDEPVTPELTSRATRGRAQRSSVKTPDPVTTTTPELQPSTSTDQLVTPKRTSRAPRGRTRRLSAKTPEPVVPTASELQPSAPADQPVGPWATQCRRHRSSVKTPEPVVPTVPEPQPSTSKDQSVTPEPTSQATESQTHRSSIKTSQPTEPTASDLKPSSPTDQLVTPKVIAQGGPSRTGRASTASAVLVPTTPEFQSPVPSEQPLPPDPIPEVNCSRRLRATRKHGSPTAHVHEPCTVPPEPNSHSSRNQRHGAVKAAKPLSTIPEPAFAQLPEAPPHTPQVPKEEAADGSGFTPEPQPRASQNHKRPSATAHSPPLQKRLQRGRVPQKAASLKEEENPVARPRKEEGVVIPGPGKRKREQTEEESQGRPSRSLRRTRPMQESTAPKVLFTGVVDARGERTVLALGGSLASSVAEASHLVTDRIRRTVKFLCALGRGIPILSLAWLHESRKAGCFLPPDEYLVTDPEQEKNFGFSLREALSRAQERRLLEGYEIHVTPGVQPPPPQMGEIISCCGGAILPSMPRSYKPQRFVITCSQDFPRCAVPYRVGLPILSPEFLLTGVLKQEVKPEAFAFSTVEMSST

Target ORF information:

RefSeq Version	XM_012164409.2
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon mediator of DNA damage checkpoint 1 (MDC1), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012164409.2

ORF Insert Sequence:

ATGGAGGTGT CCCACTTTCC TTGTCTACAT TTTCCCTTTA AGATCATGGA AGACACCCAG 
ATTTTAAACT GGGAAGTTGA AGAAGAGGAG GAGGTTGCAG AGAGCCCCAA TGAATCTCTG 
GGGTATAGCT TGGAGCCCCT AGCACAGCTG CATATCTTCA GTAGTTCCTA TGGACCAGAA 
AAAGACTTCC CACTCTACCT TGGAAAGAAC ATGATAGGCC GAATGCCTGA TTGCTCTGTG 
GCCCTGCCCT TTTCATCAAT ATCCAAACAA CATGCAGTGA TTGAAATCTC TGCCTGGGAC 
AAGGCGCCTG TCCTTCGGGA TTGCGGGAGC CTCAATGGCA CTCAAATCCT GCGGCCTCCT 
AAGGTCCTGA GCCCTGGGGT GAGTCATCGT TTGCGGGACC GGGAGTTGAT TCTCTTTGCT 
GACTTGCCCT GCCAGTACCA TCGCTTGGAT GTCCCCCGGC CTTTTGTCTC CCGGGGCCCT 
CTAACTGTAG AGGAGACACC CAGGGTACAG GGAGGAACTC AACCCTCCAG GCTTCTGTTG 
GCTGAGGACT CAGAGGAAGA AGTAGATTCC CTTTTGGACA AGTGTGTGGT GAAAGGACCA 
AGGACCTCCT CTTCGGCAAC AGTCGTTCCA GAGAGTGATG AAGAAGGGCC TTCCCCTGCC 
CCAGATGGGC CCGGGCCACC TTCTGCCTTC AACTTGAACA GCGACACGGA TGAGGAAGAA 
AGTCAGGAAT CAGGAGCAGG GGAGGCCTCT TCAGCTGCCA GAAGAGGTAC CGCTGCAGAG 
ACGGAACAGC CTGAACCTGT CACAGCCGAG ATCCAGATTG AGAAGGATCA GTGTTCCGTG 
AAGGAGAGGA ACGGGGACAC AGAAATCGAG AGGGATGTGA GGAATGGGGT GGTTCCGACT 
GGAGTGATTC TGGAGAGGAG CCAGCCTTCT GGGGAGGACA GTGACACAGA TGTGAGTGAT 
GAGAGTGGGC CTCGAAGAAG GCTTGTTGGA GTCCGTCCGA AAAGGGCCTG GTCTTGTAAC 
TTCATAGACA GTGATACCGA TGGAGAGGAC GAGGGGATCC CTGCTACCCC AGCAGTGGTT 
CCCATGAAGG AGAGGCAGAT CTTCCACGAA GCTGGTACAC AGAGCCCCCA GGCACCTGGT 
GTGGCACGTT GGCAGGAGAG CCCAGCTGAT GGTGATACAG ATATAGAGGA GGGGGAGGCC 
CCCCCAGACA GAAGCCAAGC CTCCGTGGTG ATCGACAGCA ATACAGACGA TGAGGAAGAA 
GTCTCAGCAG CACTGACACT GGCATGTCTA AGAGAGAGCC AGGCTGGTAA GTGGAACCGA 
GATCCAGATG CAGAAGAGAC CAGGGCCCAA CCCGTGGCCC TTCTGGAGCG AAGCCAGGCC 
TCTGCTGGGG GAGACAGTGA CACAGATGTG GAGGAAGAGG GGCTCCCAGT GGAAAGGAGG 
GGAATGGTTC CCAAGGGTTA CATGGACAGG GAATATTCAA AAAAGAGCCA GCACCCTCCC 
AGGGACAGTG ATACAGAGGG GAAGGAAGAT GAGAGCTCAC CGGGGGTCCA CCTGGAGAGA 
AGCCAGGCCT CTGCACAAGT GGAGGATGAG GTCCCACTGG GGCCAGCTGT TGCACTTCCG 
GAGAAGCGTC AGGTACAAGG CATAGTGTGG CCACATCACA CCGATGCGGA GGCAGAAGGG 
GACCCGGCAC AGCTCCCCGT GCTGCGTCTA GAGGAAGCCC GGCCTCCTCT AGCTGGGGAC 
TGTGAACTGG ATTCGGAGAA CACATCTGCT GTCAGAAAGA GCCAGCTTCC GGCGGAAAAA 
GATGCTGGGA CCACGTGGGC TGCAGCCGTT CCTGAACAGG ACAGAGCACT TGCCATCGGG 
ACCCAGGGTG GGTCATCCAC GGCACCAGGG GAGCAAGACC TTCTCCCGGT CTCAAGGGAA 
GACCTAGCAG ATATGGTGGT GGACATAGGC ACTCCAGGGG AGCCCCAACC GCAGAGAGAG 
GGGGCCCAGA CCACCACAGG AAGGGAGAGA GAACCATATG GGAATAGGGC CACAGACTCT 
GGAGACAACC TCCACGATTC TGAAGATCTG GACCTACCAG CTACCCAGTG CTTTGTAGAC 
AGAAAGAATC AGAGCCTGGA AGCAGCCCCC AGCATGGAGG ATGAGCCCAC CCAGGCCTTC 
CTGTTTCCTC TGCCCCAAGA GCCTGGCCCT TCCTGTTGCA GCTCCCAGGC CACAGGTTCC 
TTGGATGAGT CATGGGAGGC CTTGGCGACA CAGCCATTCT GTCCGAGAGA ATCTGAGGCC 
TCCGAGACCC AAACCGTTGT CACCCTCCTT GACACCCATG CATCTTGGCC CTCTCCATCT 
AGGACAGCAC AGCAAGAGCA ACATCCAGAG AGCCCAGTCC ATGCAGAGCC ACTGGGGATG 
GAAGGCAGAG AGATGCAGAC TGTGGAGAAA GAAATGGGTG ACTTGAATTG CGAGATGCCA 
CCTGCTCAGA AGGCTTCCAG GGGTGATCAG GAATCCCCAG ATGCTTGTCT GCCTCCTGCA 
GCGCCTGAAG CCTCAGCCGC ACTCCCAAAT TCCCTCATCA TCTCTCAGAT CCAAAAACAT 
CCCGCACCTC AGTCCCTCCT TTTTCCCTCT CTAGCTCCTT TAGAACTGCC TATTCCCAGG 
ACCAGACAAA ATGAGACTCA GGAAGCTCCA GAGACTCCCT TCTCCTCAGA GCTGAACTCT 
GTCCACCCAG AACCCAAAGT CAGGCTCCAG GCGTCCTCTC CAGTTTCTTC TCTACCCCTT 
GAGCCCCACC CTACCACCCC CACAGGCCAG CCTATTGCCC TTGAACCCAT ATCTGGGGTC 
TCTTGGAGCA GGACACGTAG TTCCTTTGAT GTAACTGCCT CATCAGTTGT CCCCACAGCC 
GTTGCACTGC AGCCATCCAT CTCCACAGAC AAGCCTGTCA CCCCTAAGCC CACACTTCGG 
GCCCCTCGGG GCAGGGCACA GAGGTCTTCT GTCAAAACCC CTGAACCCAA TGTCACCACA 
GACCAGCCTG TTGCCCCTGA GCTCACAGCT ATGGCAACTC GGGGCAGGGC ACAGAGGTCT 
TCTGTCAAGA TTCCCAAACC AGATAACCCC ACAACACCCA AGCCCCAGCC TTCCACTTCC 
ACAGACCAGC CTGTCATCCC CAAACCCACA TCTCGGGCCC CTCGGGGCAG GACACCTAGG 
TCTTCTGCCA AGACTCCTGA ACCAGCTGTC CCCACAGCCT CTGAGCTCCA GCCTGCTGCC 
CCTAAAGACC AGCCTGTTGC TCCTGAGCTC ACATCTAGAG CCACTCGGGG CAGGACACAG 
AGGTCTATCA AGACTTCCAA ACCAGATATG CCCACAACTC CCGAGCCCCA GCCTTCCACC 
TCCGCAGACC AGCCTGTCAC CCCCAAACCC ACGTCTCGGG CCCCTCGGGG CAGGACACCT 
AGGTCTTCCG CCAAGACTCC TGAGCCAGTT GTCTCCACAG CCTCTGAGCT CCAGCCTTCT 
GCCCTCACAG ATGAGCCTGT CACTCCTGAG CTCACATCTA GGGCTACTCG GGGTCGGGCA 
CAGAGGTCCT CTGTCAAAAC CCCTGATCCA GTTACCACCA CAACACCTGA GCTCCAGCCT 
TCCACCTCCA CAGACCAGCT TGTCACCCCC AAACGCACAT CTCGGGCCCC TCGAGGCAGG 
ACACGTAGGT TGTCTGCCAA GACTCCTGAA CCAGTTGTTC CCACAGCCTC TGAGCTCCAG 
CCTTCTGCCC CTGCAGACCA GCCTGTTGGT CCTTGGGCCA CTCAGTGTAG AAGACATAGG 
TCTTCTGTCA AGACCCCAGA ACCAGTTGTC CCCACAGTCC CTGAACCTCA GCCTTCCACT 
TCTAAAGACC AGTCTGTCAC TCCTGAGCCC ACATCTCAGG CCACTGAGAG CCAAACACAT 
AGGTCCTCTA TCAAGACATC CCAGCCAACG GAACCCACAG CCTCTGACCT CAAACCTTCC 
TCCCCCACAG ACCAGCTTGT CACTCCCAAG GTCATAGCTC AGGGTGGTCC AAGCAGGACA 
GGAAGGGCTT CCACAGCAAG TGCTGTGCTG GTTCCTACTA CCCCTGAATT CCAGTCTCCA 
GTCCCGTCAG AACAGCCTCT TCCCCCTGAC CCCATCCCTG AAGTCAACTG CAGCAGGAGG 
CTGAGGGCCA CTAGGAAACA TGGGTCTCCC ACAGCTCATG TTCATGAGCC CTGCACCGTA 
CCCCCTGAAC CTAACTCCCA CTCTTCAAGG AACCAAAGAC ATGGGGCAGT GAAAGCAGCC 
AAGCCCCTTA GCACCATTCC TGAGCCTGCC TTTGCCCAGC TCCCCGAGGC ACCGCCTCAC 
ACTCCCCAGG TGCCAAAGGA GGAGGCAGCA GATGGATCAG GCTTCACCCC AGAGCCCCAG 
CCTAGGGCCT CTCAAAACCA CAAGAGGCCT TCAGCTACTG CACATTCACC TCCACTTCAA 
AAACGGCTCC AGAGAGGGAG AGTCCCTCAG AAGGCAGCAT CCCTTAAGGA AGAAGAAAAT 
CCAGTAGCGA GGCCGAGGAA GGAAGAGGGT GTAGTGATTC CAGGTCCAGG CAAGAGAAAG 
AGAGAGCAGA CAGAGGAGGA GTCCCAGGGA AGACCGAGCC GCAGCCTGCG ACGGACCAGA 
CCGATGCAGG AGTCCACGGC CCCCAAAGTG CTGTTCACCG GTGTGGTGGA TGCTCGCGGA 
GAGAGGACGG TGCTGGCCCT GGGGGGCAGT TTGGCCAGCT CGGTGGCCGA GGCTTCTCAC 
CTGGTGACTG ATCGGATCCG CCGGACGGTC AAGTTTCTGT GTGCCCTGGG CCGGGGCATC 
CCCATCCTCT CCCTGGCCTG GCTGCATGAG TCCCGCAAGG CAGGCTGCTT CTTGCCCCCG 
GACGAATATT TGGTGACTGA TCCTGAGCAG GAGAAGAACT TCGGCTTCAG CCTTCGGGAG 
GCCCTGAGCC GAGCTCAGGA GCGAAGGCTG CTGGAGGGCT ATGAGATTCA TGTGACCCCC 
GGAGTCCAGC CACCGCCACC TCAGATGGGA GAAATCATCA GCTGCTGTGG AGGCGCCATC 
CTGCCCAGCA TGCCTCGGTC CTACAAGCCT CAGAGGTTCG TGATCACATG TTCCCAGGAC 
TTTCCTCGAT GTGCTGTTCC ATATCGGGTT GGGCTGCCTA TCCTCTCACC CGAATTCCTG 
CTGACGGGAG TACTGAAGCA GGAAGTCAAG CCAGAGGCCT TTGCCTTCTC CACTGTGGAA 
ATGTCATCCA CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MDC1 ( XM_012164410.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_012019800.1 Ovis aries musimon mediator of DNA damage checkpoint 1 (MDC1), transcript variant X19, mRNA.

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CloneID	OOv35153
Clone ID Related Accession (Same CDS sequence)	XM_012164410.2
Accession Version	XM_012164410.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5061bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	mediator of DNA damage checkpoint protein 1 isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011943095.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012164410.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGTGT CCCACTTTCC TTGTCTACAT TTTCCCTTTA AGATCATGGA AGACACCCAG 
ATTTTAAACT GGGAAGTTGA AGAAGAGGAG GAGGTTGCAG AGAGCCCCAA TGAATCTCTG 
GGGTATAGCT TGGAGCCCCT AGCACAGCTG CATATCTTCA GTAGTTCCTA TGGACCAGAA 
AAAGACTTCC CACTCTACCT TGGAAAGAAC ATGATAGGCC GAATGCCTGA TTGCTCTGTG 
GCCCTGCCCT TTTCATCAAT ATCCAAACAA CATGCAGTGA TTGAAATCTC TGCCTGGGAC 
AAGGCGCCTG TCCTTCGGGA TTGCGGGAGC CTCAATGGCA CTCAAATCCT GCGGCCTCCT 
AAGGTCCTGA GCCCTGGGGT GAGTCATCGT TTGCGGGACC GGGAGTTGAT TCTCTTTGCT 
GACTTGCCCT GCCAGTACCA TCGCTTGGAT GTCCCCCGGC CTTTTGTCTC CCGGGGCCCT 
CTAACTGTAG AGGAGACACC CAGGGTACAG GGAGGAACTC AACCCTCCAG GCTTCTGTTG 
GCTGAGGACT CAGAGGAAGA AGTAGATTCC CTTTTGGACA AGTGTGTGGT GAAAGGACCA 
AGGACCTCCT CTTCGGCAAC AGTCGTTCCA GAGAGTGATG AAGAAGGGCC TTCCCCTGCC 
CCAGATGGGC CCGGGCCACC TTCTGCCTTC AACTTGAACA GCGACACGGA TGAGGAAGAA 
AGTCAGGAAT CAGGAGCAGG GGAGGCCTCT TCAGCTGCCA GAAGAGGTAC CGCTGCAGAG 
ACGGAACAGC CTGAACCTGT CACAGCCGAG ATCCAGATTG AGAAGGATCA GTGTTCCGTG 
AAGGAGAGGA ACGGGGACAC AGAAATCGAG AGGGATGTGA GGAATGGGGT GGTTCCGACT 
GGAGTGATTC TGGAGAGGAG CCAGCCTTCT GGGGAGGACA GTGACACAGA TGTGAGTGAT 
GAGAGTGGGC CTCGAAGAAG GCTTGTTGGA GTCCGTCCGA AAAGGGCCTG GTCTTGTAAC 
TTCATAGACA GTGATACCGA TGGAGAGGAC GAGGGGATCC CTGCTACCCC AGCAGTGGTT 
CCCATGAAGG AGAGGCAGAT CTTCCACGAA GCTGGTACAC AGAGCCCCCA GGCACCTGGT 
GTGGCACGTT GGCAGGAGAG CCCAGCTGAT GGTGATACAG ATATAGAGGA GGGGGAGGCC 
CCCCCAGACA GAAGCCAAGC CTCCGTGGTG ATCGACAGCA ATACAGACGA TGAGGAAGAA 
GTCTCAGCAG CACTGACACT GGCATGTCTA AGAGAGAGCC AGGCTGATTC TGAAGATCTG 
GACCTACCAG CTACCCAGTG CTTTGTAGAC AGAAAGAATC AGAGCCTGGA AGCAGCCCCC 
AGCATGGAGG ATGAGCCCAC CCAGGCCTTC CTGTTTCCTC TGCCCCAAGA GCCTGGCCCT 
TCCTGTTGCA GCTCCCAGGC CACAGGTTCC TTGGATGAGT CATGGGAGGC CTTGGCGACA 
CAGCCATTCT GTCCGAGAGA ATCTGAGGCC TCCGAGACCC AAACCGTTGT CACCCTCCTT 
GACACCCATG CATCTTGGCC CTCTCCATCT AGGACAGCAC AGCAAGAGCA ACATCCAGAG 
AGCCCAGTCC ATGCAGAGCC ACTGGGGATG GAAGGCAGAG AGATGCAGAC TGTGGAGAAA 
GAAATGGGTA CCCCAAGAGA AACAGCAGAG AGGGTGATCC CTGAGGGAGG GCCACCGCAG 
GAGGAAACCA AGAAACTGCC CTCAGAAGGA GAGAGGGAAG ATGTGACGGG AGAGGAAGAA 
TTAATCGGGG GGATACAGGG CAGAGAAAAA AATCAGGTGT TTGCTAGAGA TACTCAGAGC 
CAAGAATCTG ACAAAAAAGT GAAAAGTGCA AGTACTGGAA GGGGAATGGA GATTTTAAAG 
GTAGAAATTG AGACACCCAA GGAAACACAA GAGAAAGAGA GAGAAAAGCA GACTCTCGCA 
GGGGAAATAT TTGAGAGTGA AGCAGGGAAA CTGGTAGTAG AGAGAGAGAG TGAGGCAGAT 
GGGTTAGAAG TCAAGGAACC CCAAGAGCTA CTGGACAGAA GCCCACAGAT GGGGGAGACA 
GAGGCGGGGG ACCAGGACCA GAAAGGCCAA GCCTCTGGTC TGCCATCAGA GCCTGGAGCA 
GGGGCAGGAG ACCTTCAGGG ACTTGCTTCA GACCCAGTAG CTTCTGGGAG CCAGGCAGGT 
GGAGGAAGGG GAGTCCCAGG GAGCCCCAGG AGGCAGCAGA GAGGTGACTT GAATTGCGAG 
ATGCCACCTG CTCAGAAGGC TTCCAGGGGT GATCAGGAAT CCCCAGATGC TTGTCTGCCT 
CCTGCAGCGC CTGAAGCCTC AGCCGCACTC CCAAATTCCC TCATCATCTC TCAGATCCAA 
AAACATCCCG CACCTCAGTC CCTCCTTTTT CCCTCTCTAG CTCCTTTAGA ACTGCCTATT 
CCCAGGACCA GACAAAATGA GACTCAGGAA GCTCCAGAGA CTCCCTTCTC CTCAGAGCTG 
AACTCTGTCC ACCCAGAACC CAAAGTCAGG CTCCAGGCGT CCTCTCCAGT TTCTTCTCTA 
CCCCTTGAGC CCCACCCTAC CACCCCCACA GGCCAGCCTA TTGCCCTTGA ACCCATATCT 
GGGGTCTCTT GGAGCAGGAC ACGTAGTTCC TTTGATGTAA CTGCCTCATC AGTTGTCCCC 
ACAGCCGTTG CACTGCAGCC ATCCATCTCC ACAGACAAGC CTGTCACCCC TAAGCCCACA 
CTTCGGGCCC CTCGGGGCAG GGCACAGAGG TCTTCTGTCA AAACCCCTGA ACCCAATGTC 
ACCACAGACC AGCCTGTTGC CCCTGAGCTC ACAGCTATGG CAACTCGGGG CAGGGCACAG 
AGGTCTTCTG TCAAGATTCC CAAACCAGAT AACCCCACAA CACCCAAGCC CCAGCCTTCC 
ACTTCCACAG ACCAGCCTGT CATCCCCAAA CCCACATCTC GGGCCCCTCG GGGCAGGACA 
CCTAGGTCTT CTGCCAAGAC TCCTGAACCA GCTGTCCCCA CAGCCTCTGA GCTCCAGCCT 
GCTGCCCCTA AAGACCAGCC TGTTGCTCCT GAGCTCACAT CTAGAGCCAC TCGGGGCAGG 
ACACAGAGGT CTATCAAGAC TTCCAAACCA GATATGCCCA CAACTCCCGA GCCCCAGCCT 
TCCACCTCCG CAGACCAGCC TGTCACCCCC AAACCCACGT CTCGGGCCCC TCGGGGCAGG 
ACACCTAGGT CTTCCGCCAA GACTCCTGAG CCAGTTGTCT CCACAGCCTC TGAGCTCCAG 
CCTTCTGCCC TCACAGATGA GCCTGTCACT CCTGAGCTCA CATCTAGGGC TACTCGGGGT 
CGGGCACAGA GGTCCTCTGT CAAAACCCCT GATCCAGTTA CCACCACAAC ACCTGAGCTC 
CAGCCTTCCA CCTCCACAGA CCAGCTTGTC ACCCCCAAAC GCACATCTCG GGCCCCTCGA 
GGCAGGACAC GTAGGTTGTC TGCCAAGACT CCTGAACCAG TTGTTCCCAC AGCCTCTGAG 
CTCCAGCCTT CTGCCCCTGC AGACCAGCCT GTTGGTCCTT GGGCCACTCA GTGTAGAAGA 
CATAGGTCTT CTGTCAAGAC CCCAGAACCA GTTGTCCCCA CAGTCCCTGA ACCTCAGCCT 
TCCACTTCTA AAGACCAGTC TGTCACTCCT GAGCCCACAT CTCAGGCCAC TGAGAGCCAA 
ACACATAGGT CCTCTATCAA GACATCCCAG CCAACGGAAC CCACAGCCTC TGACCTCAAA 
CCTTCCTCCC CCACAGACCA GCTTGTCACT CCCAAGGTCA TAGCTCAGGG TGGTCCAAGC 
AGGACAGGAA GGGCTTCCAC AGCAAGTGCT GTGCTGGTTC CTACTACCCC TGAATTCCAG 
TCTCCAGTCC CGTCAGAACA GCCTCTTCCC CCTGACCCCA TCCCTGAAGT CAACTGCAGC 
AGGAGGCTGA GGGCCACTAG GAAACATGGG TCTCCCACAG CTCATGTTCA TGAGCCCTGC 
ACCGTACCCC CTGAACCTAA CTCCCACTCT TCAAGGAACC AAAGACATGG GGCAGTGAAA 
GCAGCCAAGC CCCTTAGCAC CATTCCTGAG CCTGCCTTTG CCCAGCTCCC CGAGGCACCG 
CCTCACACTC CCCAGGTGCC AAAGGAGGAG GCAGCAGATG GATCAGGCTT CACCCCAGAG 
CCCCAGCCTA GGGCCTCTCA AAACCACAAG AGGCCTTCAG CTACTGCACA TTCACCTCCA 
CTTCAAAAAC GGCTCCAGAG AGGGAGAGTC CCTCAGAAGG CAGCATCCCT TAAGGAAGAA 
GAAAATCCAG TAGCGAGGCC GAGGAAGGAA GAGGGTGTAG TGATTCCAGG TCCAGGCAAG 
AGAAAGAGAG AGCAGACAGA GGAGGAGTCC CAGGGAAGAC CGAGCCGCAG CCTGCGACGG 
ACCAGACCGA TGCAGGAGTC CACGGCCCCC AAAGTGCTGT TCACCGGTGT GGTGGATGCT 
CGCGGAGAGA GGACGGTGCT GGCCCTGGGG GGCAGTTTGG CCAGCTCGGT GGCCGAGGCT 
TCTCACCTGG TGACTGATCG GATCCGCCGG ACGGTCAAGT TTCTGTGTGC CCTGGGCCGG 
GGCATCCCCA TCCTCTCCCT GGCCTGGCTG CATGAGTCCC GCAAGGCAGG CTGCTTCTTG 
CCCCCGGACG AATATTTGGT GACTGATCCT GAGCAGGAGA AGAACTTCGG CTTCAGCCTT 
CGGGAGGCCC TGAGCCGAGC TCAGGAGCGA AGGCTGCTGG AGGGCTATGA GATTCATGTG 
ACCCCCGGAG TCCAGCCACC GCCACCTCAG ATGGGAGAAA TCATCAGCTG CTGTGGAGGC 
GCCATCCTGC CCAGCATGCC TCGGTCCTAC AAGCCTCAGA GGTTCGTGAT CACATGTTCC 
CAGGACTTTC CTCGATGTGC TGTTCCATAT CGGGTTGGGC TGCCTATCCT CTCACCCGAA 
TTCCTGCTGA CGGGAGTACT GAAGCAGGAA GTCAAGCCAG AGGCCTTTGC CTTCTCCACT 
GTGGAAATGT CATCCACCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012019800.1
CDS	328..5388
Translation	MEVSHFPCLHFPFKIMEDTQILNWEVEEEEEVAESPNESLGYSLEPLAQLHIFSSSYGPEKDFPLYLGKNMIGRMPDCSVALPFSSISKQHAVIEISAWDKAPVLRDCGSLNGTQILRPPKVLSPGVSHRLRDRELILFADLPCQYHRLDVPRPFVSRGPLTVEETPRVQGGTQPSRLLLAEDSEEEVDSLLDKCVVKGPRTSSSATVVPESDEEGPSPAPDGPGPPSAFNLNSDTDEEESQESGAGEASSAARRGTAAETEQPEPVTAEIQIEKDQCSVKERNGDTEIERDVRNGVVPTGVILERSQPSGEDSDTDVSDESGPRRRLVGVRPKRAWSCNFIDSDTDGEDEGIPATPAVVPMKERQIFHEAGTQSPQAPGVARWQESPADGDTDIEEGEAPPDRSQASVVIDSNTDDEEEVSAALTLACLRESQADSEDLDLPATQCFVDRKNQSLEAAPSMEDEPTQAFLFPLPQEPGPSCCSSQATGSLDESWEALATQPFCPRESEASETQTVVTLLDTHASWPSPSRTAQQEQHPESPVHAEPLGMEGREMQTVEKEMGTPRETAERVIPEGGPPQEETKKLPSEGEREDVTGEEELIGGIQGREKNQVFARDTQSQESDKKVKSASTGRGMEILKVEIETPKETQEKEREKQTLAGEIFESEAGKLVVERESEADGLEVKEPQELLDRSPQMGETEAGDQDQKGQASGLPSEPGAGAGDLQGLASDPVASGSQAGGGRGVPGSPRRQQRGDLNCEMPPAQKASRGDQESPDACLPPAAPEASAALPNSLIISQIQKHPAPQSLLFPSLAPLELPIPRTRQNETQEAPETPFSSELNSVHPEPKVRLQASSPVSSLPLEPHPTTPTGQPIALEPISGVSWSRTRSSFDVTASSVVPTAVALQPSISTDKPVTPKPTLRAPRGRAQRSSVKTPEPNVTTDQPVAPELTAMATRGRAQRSSVKIPKPDNPTTPKPQPSTSTDQPVIPKPTSRAPRGRTPRSSAKTPEPAVPTASELQPAAPKDQPVAPELTSRATRGRTQRSIKTSKPDMPTTPEPQPSTSADQPVTPKPTSRAPRGRTPRSSAKTPEPVVSTASELQPSALTDEPVTPELTSRATRGRAQRSSVKTPDPVTTTTPELQPSTSTDQLVTPKRTSRAPRGRTRRLSAKTPEPVVPTASELQPSAPADQPVGPWATQCRRHRSSVKTPEPVVPTVPEPQPSTSKDQSVTPEPTSQATESQTHRSSIKTSQPTEPTASDLKPSSPTDQLVTPKVIAQGGPSRTGRASTASAVLVPTTPEFQSPVPSEQPLPPDPIPEVNCSRRLRATRKHGSPTAHVHEPCTVPPEPNSHSSRNQRHGAVKAAKPLSTIPEPAFAQLPEAPPHTPQVPKEEAADGSGFTPEPQPRASQNHKRPSATAHSPPLQKRLQRGRVPQKAASLKEEENPVARPRKEEGVVIPGPGKRKREQTEEESQGRPSRSLRRTRPMQESTAPKVLFTGVVDARGERTVLALGGSLASSVAEASHLVTDRIRRTVKFLCALGRGIPILSLAWLHESRKAGCFLPPDEYLVTDPEQEKNFGFSLREALSRAQERRLLEGYEIHVTPGVQPPPPQMGEIISCCGGAILPSMPRSYKPQRFVITCSQDFPRCAVPYRVGLPILSPEFLLTGVLKQEVKPEAFAFSTVEMSST

Target ORF information:

RefSeq Version	XM_012164410.2
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon mediator of DNA damage checkpoint 1 (MDC1), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012164410.2

ORF Insert Sequence:

ATGGAGGTGT CCCACTTTCC TTGTCTACAT TTTCCCTTTA AGATCATGGA AGACACCCAG 
ATTTTAAACT GGGAAGTTGA AGAAGAGGAG GAGGTTGCAG AGAGCCCCAA TGAATCTCTG 
GGGTATAGCT TGGAGCCCCT AGCACAGCTG CATATCTTCA GTAGTTCCTA TGGACCAGAA 
AAAGACTTCC CACTCTACCT TGGAAAGAAC ATGATAGGCC GAATGCCTGA TTGCTCTGTG 
GCCCTGCCCT TTTCATCAAT ATCCAAACAA CATGCAGTGA TTGAAATCTC TGCCTGGGAC 
AAGGCGCCTG TCCTTCGGGA TTGCGGGAGC CTCAATGGCA CTCAAATCCT GCGGCCTCCT 
AAGGTCCTGA GCCCTGGGGT GAGTCATCGT TTGCGGGACC GGGAGTTGAT TCTCTTTGCT 
GACTTGCCCT GCCAGTACCA TCGCTTGGAT GTCCCCCGGC CTTTTGTCTC CCGGGGCCCT 
CTAACTGTAG AGGAGACACC CAGGGTACAG GGAGGAACTC AACCCTCCAG GCTTCTGTTG 
GCTGAGGACT CAGAGGAAGA AGTAGATTCC CTTTTGGACA AGTGTGTGGT GAAAGGACCA 
AGGACCTCCT CTTCGGCAAC AGTCGTTCCA GAGAGTGATG AAGAAGGGCC TTCCCCTGCC 
CCAGATGGGC CCGGGCCACC TTCTGCCTTC AACTTGAACA GCGACACGGA TGAGGAAGAA 
AGTCAGGAAT CAGGAGCAGG GGAGGCCTCT TCAGCTGCCA GAAGAGGTAC CGCTGCAGAG 
ACGGAACAGC CTGAACCTGT CACAGCCGAG ATCCAGATTG AGAAGGATCA GTGTTCCGTG 
AAGGAGAGGA ACGGGGACAC AGAAATCGAG AGGGATGTGA GGAATGGGGT GGTTCCGACT 
GGAGTGATTC TGGAGAGGAG CCAGCCTTCT GGGGAGGACA GTGACACAGA TGTGAGTGAT 
GAGAGTGGGC CTCGAAGAAG GCTTGTTGGA GTCCGTCCGA AAAGGGCCTG GTCTTGTAAC 
TTCATAGACA GTGATACCGA TGGAGAGGAC GAGGGGATCC CTGCTACCCC AGCAGTGGTT 
CCCATGAAGG AGAGGCAGAT CTTCCACGAA GCTGGTACAC AGAGCCCCCA GGCACCTGGT 
GTGGCACGTT GGCAGGAGAG CCCAGCTGAT GGTGATACAG ATATAGAGGA GGGGGAGGCC 
CCCCCAGACA GAAGCCAAGC CTCCGTGGTG ATCGACAGCA ATACAGACGA TGAGGAAGAA 
GTCTCAGCAG CACTGACACT GGCATGTCTA AGAGAGAGCC AGGCTGATTC TGAAGATCTG 
GACCTACCAG CTACCCAGTG CTTTGTAGAC AGAAAGAATC AGAGCCTGGA AGCAGCCCCC 
AGCATGGAGG ATGAGCCCAC CCAGGCCTTC CTGTTTCCTC TGCCCCAAGA GCCTGGCCCT 
TCCTGTTGCA GCTCCCAGGC CACAGGTTCC TTGGATGAGT CATGGGAGGC CTTGGCGACA 
CAGCCATTCT GTCCGAGAGA ATCTGAGGCC TCCGAGACCC AAACCGTTGT CACCCTCCTT 
GACACCCATG CATCTTGGCC CTCTCCATCT AGGACAGCAC AGCAAGAGCA ACATCCAGAG 
AGCCCAGTCC ATGCAGAGCC ACTGGGGATG GAAGGCAGAG AGATGCAGAC TGTGGAGAAA 
GAAATGGGTA CCCCAAGAGA AACAGCAGAG AGGGTGATCC CTGAGGGAGG GCCACCGCAG 
GAGGAAACCA AGAAACTGCC CTCAGAAGGA GAGAGGGAAG ATGTGACGGG AGAGGAAGAA 
TTAATCGGGG GGATACAGGG CAGAGAAAAA AATCAGGTGT TTGCTAGAGA TACTCAGAGC 
CAAGAATCTG ACAAAAAAGT GAAAAGTGCA AGTACTGGAA GGGGAATGGA GATTTTAAAG 
GTAGAAATTG AGACACCCAA GGAAACACAA GAGAAAGAGA GAGAAAAGCA GACTCTCGCA 
GGGGAAATAT TTGAGAGTGA AGCAGGGAAA CTGGTAGTAG AGAGAGAGAG TGAGGCAGAT 
GGGTTAGAAG TCAAGGAACC CCAAGAGCTA CTGGACAGAA GCCCACAGAT GGGGGAGACA 
GAGGCGGGGG ACCAGGACCA GAAAGGCCAA GCCTCTGGTC TGCCATCAGA GCCTGGAGCA 
GGGGCAGGAG ACCTTCAGGG ACTTGCTTCA GACCCAGTAG CTTCTGGGAG CCAGGCAGGT 
GGAGGAAGGG GAGTCCCAGG GAGCCCCAGG AGGCAGCAGA GAGGTGACTT GAATTGCGAG 
ATGCCACCTG CTCAGAAGGC TTCCAGGGGT GATCAGGAAT CCCCAGATGC TTGTCTGCCT 
CCTGCAGCGC CTGAAGCCTC AGCCGCACTC CCAAATTCCC TCATCATCTC TCAGATCCAA 
AAACATCCCG CACCTCAGTC CCTCCTTTTT CCCTCTCTAG CTCCTTTAGA ACTGCCTATT 
CCCAGGACCA GACAAAATGA GACTCAGGAA GCTCCAGAGA CTCCCTTCTC CTCAGAGCTG 
AACTCTGTCC ACCCAGAACC CAAAGTCAGG CTCCAGGCGT CCTCTCCAGT TTCTTCTCTA 
CCCCTTGAGC CCCACCCTAC CACCCCCACA GGCCAGCCTA TTGCCCTTGA ACCCATATCT 
GGGGTCTCTT GGAGCAGGAC ACGTAGTTCC TTTGATGTAA CTGCCTCATC AGTTGTCCCC 
ACAGCCGTTG CACTGCAGCC ATCCATCTCC ACAGACAAGC CTGTCACCCC TAAGCCCACA 
CTTCGGGCCC CTCGGGGCAG GGCACAGAGG TCTTCTGTCA AAACCCCTGA ACCCAATGTC 
ACCACAGACC AGCCTGTTGC CCCTGAGCTC ACAGCTATGG CAACTCGGGG CAGGGCACAG 
AGGTCTTCTG TCAAGATTCC CAAACCAGAT AACCCCACAA CACCCAAGCC CCAGCCTTCC 
ACTTCCACAG ACCAGCCTGT CATCCCCAAA CCCACATCTC GGGCCCCTCG GGGCAGGACA 
CCTAGGTCTT CTGCCAAGAC TCCTGAACCA GCTGTCCCCA CAGCCTCTGA GCTCCAGCCT 
GCTGCCCCTA AAGACCAGCC TGTTGCTCCT GAGCTCACAT CTAGAGCCAC TCGGGGCAGG 
ACACAGAGGT CTATCAAGAC TTCCAAACCA GATATGCCCA CAACTCCCGA GCCCCAGCCT 
TCCACCTCCG CAGACCAGCC TGTCACCCCC AAACCCACGT CTCGGGCCCC TCGGGGCAGG 
ACACCTAGGT CTTCCGCCAA GACTCCTGAG CCAGTTGTCT CCACAGCCTC TGAGCTCCAG 
CCTTCTGCCC TCACAGATGA GCCTGTCACT CCTGAGCTCA CATCTAGGGC TACTCGGGGT 
CGGGCACAGA GGTCCTCTGT CAAAACCCCT GATCCAGTTA CCACCACAAC ACCTGAGCTC 
CAGCCTTCCA CCTCCACAGA CCAGCTTGTC ACCCCCAAAC GCACATCTCG GGCCCCTCGA 
GGCAGGACAC GTAGGTTGTC TGCCAAGACT CCTGAACCAG TTGTTCCCAC AGCCTCTGAG 
CTCCAGCCTT CTGCCCCTGC AGACCAGCCT GTTGGTCCTT GGGCCACTCA GTGTAGAAGA 
CATAGGTCTT CTGTCAAGAC CCCAGAACCA GTTGTCCCCA CAGTCCCTGA ACCTCAGCCT 
TCCACTTCTA AAGACCAGTC TGTCACTCCT GAGCCCACAT CTCAGGCCAC TGAGAGCCAA 
ACACATAGGT CCTCTATCAA GACATCCCAG CCAACGGAAC CCACAGCCTC TGACCTCAAA 
CCTTCCTCCC CCACAGACCA GCTTGTCACT CCCAAGGTCA TAGCTCAGGG TGGTCCAAGC 
AGGACAGGAA GGGCTTCCAC AGCAAGTGCT GTGCTGGTTC CTACTACCCC TGAATTCCAG 
TCTCCAGTCC CGTCAGAACA GCCTCTTCCC CCTGACCCCA TCCCTGAAGT CAACTGCAGC 
AGGAGGCTGA GGGCCACTAG GAAACATGGG TCTCCCACAG CTCATGTTCA TGAGCCCTGC 
ACCGTACCCC CTGAACCTAA CTCCCACTCT TCAAGGAACC AAAGACATGG GGCAGTGAAA 
GCAGCCAAGC CCCTTAGCAC CATTCCTGAG CCTGCCTTTG CCCAGCTCCC CGAGGCACCG 
CCTCACACTC CCCAGGTGCC AAAGGAGGAG GCAGCAGATG GATCAGGCTT CACCCCAGAG 
CCCCAGCCTA GGGCCTCTCA AAACCACAAG AGGCCTTCAG CTACTGCACA TTCACCTCCA 
CTTCAAAAAC GGCTCCAGAG AGGGAGAGTC CCTCAGAAGG CAGCATCCCT TAAGGAAGAA 
GAAAATCCAG TAGCGAGGCC GAGGAAGGAA GAGGGTGTAG TGATTCCAGG TCCAGGCAAG 
AGAAAGAGAG AGCAGACAGA GGAGGAGTCC CAGGGAAGAC CGAGCCGCAG CCTGCGACGG 
ACCAGACCGA TGCAGGAGTC CACGGCCCCC AAAGTGCTGT TCACCGGTGT GGTGGATGCT 
CGCGGAGAGA GGACGGTGCT GGCCCTGGGG GGCAGTTTGG CCAGCTCGGT GGCCGAGGCT 
TCTCACCTGG TGACTGATCG GATCCGCCGG ACGGTCAAGT TTCTGTGTGC CCTGGGCCGG 
GGCATCCCCA TCCTCTCCCT GGCCTGGCTG CATGAGTCCC GCAAGGCAGG CTGCTTCTTG 
CCCCCGGACG AATATTTGGT GACTGATCCT GAGCAGGAGA AGAACTTCGG CTTCAGCCTT 
CGGGAGGCCC TGAGCCGAGC TCAGGAGCGA AGGCTGCTGG AGGGCTATGA GATTCATGTG 
ACCCCCGGAG TCCAGCCACC GCCACCTCAG ATGGGAGAAA TCATCAGCTG CTGTGGAGGC 
GCCATCCTGC CCAGCATGCC TCGGTCCTAC AAGCCTCAGA GGTTCGTGAT CACATGTTCC 
CAGGACTTTC CTCGATGTGC TGTTCCATAT CGGGTTGGGC TGCCTATCCT CTCACCCGAA 
TTCCTGCTGA CGGGAGTACT GAAGCAGGAA GTCAAGCCAG AGGCCTTTGC CTTCTCCACT 
GTGGAAATGT CATCCACCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MDC1 ( XM_012164411.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_012019801.1 Ovis aries musimon mediator of DNA damage checkpoint 1 (MDC1), transcript variant X20, mRNA.

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CloneID	OOv35154
Clone ID Related Accession (Same CDS sequence)	XM_012164411.2
Accession Version	XM_012164411.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5058bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	mediator of DNA damage checkpoint protein 1 isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011943095.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012164411.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGTGT CCCACTTTCC TTGTCTACAT TTTCCCTTTA AGATCATGGA AGACACCCAG 
ATTTTAAACT GGGAAGTTGA AGAAGAGGAG GAGGTTGCAG AGAGCCCCAA TGAATCTCTG 
GGGTATAGCT TGGAGCCCCT AGCACAGCTG CATATCTTCA GTAGTTCCTA TGGACCAGAA 
AAAGACTTCC CACTCTACCT TGGAAAGAAC ATGATAGGCC GAATGCCTGA TTGCTCTGTG 
GCCCTGCCCT TTTCATCAAT ATCCAAACAA CATGCAGTGA TTGAAATCTC TGCCTGGGAC 
AAGGCGCCTG TCCTTCGGGA TTGCGGGAGC CTCAATGGCA CTCAAATCCT GCGGCCTCCT 
AAGGTCCTGA GCCCTGGGGT GAGTCATCGT TTGCGGGACC GGGAGTTGAT TCTCTTTGCT 
GACTTGCCCT GCCAGTACCA TCGCTTGGAT GTCCCCCGGC CTTTTGTCTC CCGGGGCCCT 
CTAACTGTAG AGGAGACACC CAGGGTACAG GGAGGAACTC AACCCTCCAG GCTTCTGTTG 
GCTGAGGACT CAGAGGAAGA AGTAGATTCC CTTTTGGACA AGTGTGTGGT GAAAGGACCA 
AGGACCTCCT CTTCGGCAAC AGTCGTTCCA GAGAGTGATG AAGAAGGGCC TTCCCCTGCC 
CCAGATGGGC CCGGGCCACC TTCTGCCTTC AACTTGAACA GCGACACGGA TGAGGAAGAA 
AGTCAGGAAT CAGGAGCAGG GGAGGCCTCT TCAGCTGCCA GAAGAGGTAC CGCTGCAGAG 
ACGGAACAGC CTGAACCTGT CACAGCCGAG ATCCAGATTG AGAAGGATCA GTGTTCCGTG 
AAGGAGAGGA ACGGGGACAC AGAAATCGAG AGGGATGTGA GGAATGGGGT GGTTCCGACT 
GGAGTGATTC TGGAGAGGAG CCAGCCTTCT GGGGAGGACA GTGACACAGA TGTGAGTGAT 
GAGAGTGGGC CTCGAAGAAG GCTTGTTGGA GTCCGTCCGA AAAGGGCCTG GTCTTGTAAC 
TTCATAGACA GTGATACCGA TGGAGAGGAC GAGGGGATCC CTGCTACCCC AGCAGTGGTT 
CCCATGAAGG AGAGGCAGAT CTTCCACGAA GCTGGTACAC AGAGCCCCCA GGCACCTGGT 
GTGGCACGTT GGCAGGAGAG CCCAGCTGAT GGTGATACAG ATATAGAGGA GGGGGAGGCC 
CCCCCAGACA GAAGCCAAGC CTCCGTGGTG ATCGACAGCA ATACAGACGA TGAGGAAGAA 
GTCTCAGCAG CACTGACACT GGCATGTCTA AGAGAGAGCC AGGCTGATTC TGAAGATCTG 
GACCTACCAG CTACCCAGTG CTTTGTAGAC AGAAAGAATC AGAGCCTGGA AGCCCCCAGC 
ATGGAGGATG AGCCCACCCA GGCCTTCCTG TTTCCTCTGC CCCAAGAGCC TGGCCCTTCC 
TGTTGCAGCT CCCAGGCCAC AGGTTCCTTG GATGAGTCAT GGGAGGCCTT GGCGACACAG 
CCATTCTGTC CGAGAGAATC TGAGGCCTCC GAGACCCAAA CCGTTGTCAC CCTCCTTGAC 
ACCCATGCAT CTTGGCCCTC TCCATCTAGG ACAGCACAGC AAGAGCAACA TCCAGAGAGC 
CCAGTCCATG CAGAGCCACT GGGGATGGAA GGCAGAGAGA TGCAGACTGT GGAGAAAGAA 
ATGGGTACCC CAAGAGAAAC AGCAGAGAGG GTGATCCCTG AGGGAGGGCC ACCGCAGGAG 
GAAACCAAGA AACTGCCCTC AGAAGGAGAG AGGGAAGATG TGACGGGAGA GGAAGAATTA 
ATCGGGGGGA TACAGGGCAG AGAAAAAAAT CAGGTGTTTG CTAGAGATAC TCAGAGCCAA 
GAATCTGACA AAAAAGTGAA AAGTGCAAGT ACTGGAAGGG GAATGGAGAT TTTAAAGGTA 
GAAATTGAGA CACCCAAGGA AACACAAGAG AAAGAGAGAG AAAAGCAGAC TCTCGCAGGG 
GAAATATTTG AGAGTGAAGC AGGGAAACTG GTAGTAGAGA GAGAGAGTGA GGCAGATGGG 
TTAGAAGTCA AGGAACCCCA AGAGCTACTG GACAGAAGCC CACAGATGGG GGAGACAGAG 
GCGGGGGACC AGGACCAGAA AGGCCAAGCC TCTGGTCTGC CATCAGAGCC TGGAGCAGGG 
GCAGGAGACC TTCAGGGACT TGCTTCAGAC CCAGTAGCTT CTGGGAGCCA GGCAGGTGGA 
GGAAGGGGAG TCCCAGGGAG CCCCAGGAGG CAGCAGAGAG GTGACTTGAA TTGCGAGATG 
CCACCTGCTC AGAAGGCTTC CAGGGGTGAT CAGGAATCCC CAGATGCTTG TCTGCCTCCT 
GCAGCGCCTG AAGCCTCAGC CGCACTCCCA AATTCCCTCA TCATCTCTCA GATCCAAAAA 
CATCCCGCAC CTCAGTCCCT CCTTTTTCCC TCTCTAGCTC CTTTAGAACT GCCTATTCCC 
AGGACCAGAC AAAATGAGAC TCAGGAAGCT CCAGAGACTC CCTTCTCCTC AGAGCTGAAC 
TCTGTCCACC CAGAACCCAA AGTCAGGCTC CAGGCGTCCT CTCCAGTTTC TTCTCTACCC 
CTTGAGCCCC ACCCTACCAC CCCCACAGGC CAGCCTATTG CCCTTGAACC CATATCTGGG 
GTCTCTTGGA GCAGGACACG TAGTTCCTTT GATGTAACTG CCTCATCAGT TGTCCCCACA 
GCCGTTGCAC TGCAGCCATC CATCTCCACA GACAAGCCTG TCACCCCTAA GCCCACACTT 
CGGGCCCCTC GGGGCAGGGC ACAGAGGTCT TCTGTCAAAA CCCCTGAACC CAATGTCACC 
ACAGACCAGC CTGTTGCCCC TGAGCTCACA GCTATGGCAA CTCGGGGCAG GGCACAGAGG 
TCTTCTGTCA AGATTCCCAA ACCAGATAAC CCCACAACAC CCAAGCCCCA GCCTTCCACT 
TCCACAGACC AGCCTGTCAT CCCCAAACCC ACATCTCGGG CCCCTCGGGG CAGGACACCT 
AGGTCTTCTG CCAAGACTCC TGAACCAGCT GTCCCCACAG CCTCTGAGCT CCAGCCTGCT 
GCCCCTAAAG ACCAGCCTGT TGCTCCTGAG CTCACATCTA GAGCCACTCG GGGCAGGACA 
CAGAGGTCTA TCAAGACTTC CAAACCAGAT ATGCCCACAA CTCCCGAGCC CCAGCCTTCC 
ACCTCCGCAG ACCAGCCTGT CACCCCCAAA CCCACGTCTC GGGCCCCTCG GGGCAGGACA 
CCTAGGTCTT CCGCCAAGAC TCCTGAGCCA GTTGTCTCCA CAGCCTCTGA GCTCCAGCCT 
TCTGCCCTCA CAGATGAGCC TGTCACTCCT GAGCTCACAT CTAGGGCTAC TCGGGGTCGG 
GCACAGAGGT CCTCTGTCAA AACCCCTGAT CCAGTTACCA CCACAACACC TGAGCTCCAG 
CCTTCCACCT CCACAGACCA GCTTGTCACC CCCAAACGCA CATCTCGGGC CCCTCGAGGC 
AGGACACGTA GGTTGTCTGC CAAGACTCCT GAACCAGTTG TTCCCACAGC CTCTGAGCTC 
CAGCCTTCTG CCCCTGCAGA CCAGCCTGTT GGTCCTTGGG CCACTCAGTG TAGAAGACAT 
AGGTCTTCTG TCAAGACCCC AGAACCAGTT GTCCCCACAG TCCCTGAACC TCAGCCTTCC 
ACTTCTAAAG ACCAGTCTGT CACTCCTGAG CCCACATCTC AGGCCACTGA GAGCCAAACA 
CATAGGTCCT CTATCAAGAC ATCCCAGCCA ACGGAACCCA CAGCCTCTGA CCTCAAACCT 
TCCTCCCCCA CAGACCAGCT TGTCACTCCC AAGGTCATAG CTCAGGGTGG TCCAAGCAGG 
ACAGGAAGGG CTTCCACAGC AAGTGCTGTG CTGGTTCCTA CTACCCCTGA ATTCCAGTCT 
CCAGTCCCGT CAGAACAGCC TCTTCCCCCT GACCCCATCC CTGAAGTCAA CTGCAGCAGG 
AGGCTGAGGG CCACTAGGAA ACATGGGTCT CCCACAGCTC ATGTTCATGA GCCCTGCACC 
GTACCCCCTG AACCTAACTC CCACTCTTCA AGGAACCAAA GACATGGGGC AGTGAAAGCA 
GCCAAGCCCC TTAGCACCAT TCCTGAGCCT GCCTTTGCCC AGCTCCCCGA GGCACCGCCT 
CACACTCCCC AGGTGCCAAA GGAGGAGGCA GCAGATGGAT CAGGCTTCAC CCCAGAGCCC 
CAGCCTAGGG CCTCTCAAAA CCACAAGAGG CCTTCAGCTA CTGCACATTC ACCTCCACTT 
CAAAAACGGC TCCAGAGAGG GAGAGTCCCT CAGAAGGCAG CATCCCTTAA GGAAGAAGAA 
AATCCAGTAG CGAGGCCGAG GAAGGAAGAG GGTGTAGTGA TTCCAGGTCC AGGCAAGAGA 
AAGAGAGAGC AGACAGAGGA GGAGTCCCAG GGAAGACCGA GCCGCAGCCT GCGACGGACC 
AGACCGATGC AGGAGTCCAC GGCCCCCAAA GTGCTGTTCA CCGGTGTGGT GGATGCTCGC 
GGAGAGAGGA CGGTGCTGGC CCTGGGGGGC AGTTTGGCCA GCTCGGTGGC CGAGGCTTCT 
CACCTGGTGA CTGATCGGAT CCGCCGGACG GTCAAGTTTC TGTGTGCCCT GGGCCGGGGC 
ATCCCCATCC TCTCCCTGGC CTGGCTGCAT GAGTCCCGCA AGGCAGGCTG CTTCTTGCCC 
CCGGACGAAT ATTTGGTGAC TGATCCTGAG CAGGAGAAGA ACTTCGGCTT CAGCCTTCGG 
GAGGCCCTGA GCCGAGCTCA GGAGCGAAGG CTGCTGGAGG GCTATGAGAT TCATGTGACC 
CCCGGAGTCC AGCCACCGCC ACCTCAGATG GGAGAAATCA TCAGCTGCTG TGGAGGCGCC 
ATCCTGCCCA GCATGCCTCG GTCCTACAAG CCTCAGAGGT TCGTGATCAC ATGTTCCCAG 
GACTTTCCTC GATGTGCTGT TCCATATCGG GTTGGGCTGC CTATCCTCTC ACCCGAATTC 
CTGCTGACGG GAGTACTGAA GCAGGAAGTC AAGCCAGAGG CCTTTGCCTT CTCCACTGTG 
GAAATGTCAT CCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012019801.1
CDS	328..5385
Translation	MEVSHFPCLHFPFKIMEDTQILNWEVEEEEEVAESPNESLGYSLEPLAQLHIFSSSYGPEKDFPLYLGKNMIGRMPDCSVALPFSSISKQHAVIEISAWDKAPVLRDCGSLNGTQILRPPKVLSPGVSHRLRDRELILFADLPCQYHRLDVPRPFVSRGPLTVEETPRVQGGTQPSRLLLAEDSEEEVDSLLDKCVVKGPRTSSSATVVPESDEEGPSPAPDGPGPPSAFNLNSDTDEEESQESGAGEASSAARRGTAAETEQPEPVTAEIQIEKDQCSVKERNGDTEIERDVRNGVVPTGVILERSQPSGEDSDTDVSDESGPRRRLVGVRPKRAWSCNFIDSDTDGEDEGIPATPAVVPMKERQIFHEAGTQSPQAPGVARWQESPADGDTDIEEGEAPPDRSQASVVIDSNTDDEEEVSAALTLACLRESQADSEDLDLPATQCFVDRKNQSLEAPSMEDEPTQAFLFPLPQEPGPSCCSSQATGSLDESWEALATQPFCPRESEASETQTVVTLLDTHASWPSPSRTAQQEQHPESPVHAEPLGMEGREMQTVEKEMGTPRETAERVIPEGGPPQEETKKLPSEGEREDVTGEEELIGGIQGREKNQVFARDTQSQESDKKVKSASTGRGMEILKVEIETPKETQEKEREKQTLAGEIFESEAGKLVVERESEADGLEVKEPQELLDRSPQMGETEAGDQDQKGQASGLPSEPGAGAGDLQGLASDPVASGSQAGGGRGVPGSPRRQQRGDLNCEMPPAQKASRGDQESPDACLPPAAPEASAALPNSLIISQIQKHPAPQSLLFPSLAPLELPIPRTRQNETQEAPETPFSSELNSVHPEPKVRLQASSPVSSLPLEPHPTTPTGQPIALEPISGVSWSRTRSSFDVTASSVVPTAVALQPSISTDKPVTPKPTLRAPRGRAQRSSVKTPEPNVTTDQPVAPELTAMATRGRAQRSSVKIPKPDNPTTPKPQPSTSTDQPVIPKPTSRAPRGRTPRSSAKTPEPAVPTASELQPAAPKDQPVAPELTSRATRGRTQRSIKTSKPDMPTTPEPQPSTSADQPVTPKPTSRAPRGRTPRSSAKTPEPVVSTASELQPSALTDEPVTPELTSRATRGRAQRSSVKTPDPVTTTTPELQPSTSTDQLVTPKRTSRAPRGRTRRLSAKTPEPVVPTASELQPSAPADQPVGPWATQCRRHRSSVKTPEPVVPTVPEPQPSTSKDQSVTPEPTSQATESQTHRSSIKTSQPTEPTASDLKPSSPTDQLVTPKVIAQGGPSRTGRASTASAVLVPTTPEFQSPVPSEQPLPPDPIPEVNCSRRLRATRKHGSPTAHVHEPCTVPPEPNSHSSRNQRHGAVKAAKPLSTIPEPAFAQLPEAPPHTPQVPKEEAADGSGFTPEPQPRASQNHKRPSATAHSPPLQKRLQRGRVPQKAASLKEEENPVARPRKEEGVVIPGPGKRKREQTEEESQGRPSRSLRRTRPMQESTAPKVLFTGVVDARGERTVLALGGSLASSVAEASHLVTDRIRRTVKFLCALGRGIPILSLAWLHESRKAGCFLPPDEYLVTDPEQEKNFGFSLREALSRAQERRLLEGYEIHVTPGVQPPPPQMGEIISCCGGAILPSMPRSYKPQRFVITCSQDFPRCAVPYRVGLPILSPEFLLTGVLKQEVKPEAFAFSTVEMSST

Target ORF information:

RefSeq Version	XM_012164411.2
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon mediator of DNA damage checkpoint 1 (MDC1), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012164411.2

ORF Insert Sequence:

ATGGAGGTGT CCCACTTTCC TTGTCTACAT TTTCCCTTTA AGATCATGGA AGACACCCAG 
ATTTTAAACT GGGAAGTTGA AGAAGAGGAG GAGGTTGCAG AGAGCCCCAA TGAATCTCTG 
GGGTATAGCT TGGAGCCCCT AGCACAGCTG CATATCTTCA GTAGTTCCTA TGGACCAGAA 
AAAGACTTCC CACTCTACCT TGGAAAGAAC ATGATAGGCC GAATGCCTGA TTGCTCTGTG 
GCCCTGCCCT TTTCATCAAT ATCCAAACAA CATGCAGTGA TTGAAATCTC TGCCTGGGAC 
AAGGCGCCTG TCCTTCGGGA TTGCGGGAGC CTCAATGGCA CTCAAATCCT GCGGCCTCCT 
AAGGTCCTGA GCCCTGGGGT GAGTCATCGT TTGCGGGACC GGGAGTTGAT TCTCTTTGCT 
GACTTGCCCT GCCAGTACCA TCGCTTGGAT GTCCCCCGGC CTTTTGTCTC CCGGGGCCCT 
CTAACTGTAG AGGAGACACC CAGGGTACAG GGAGGAACTC AACCCTCCAG GCTTCTGTTG 
GCTGAGGACT CAGAGGAAGA AGTAGATTCC CTTTTGGACA AGTGTGTGGT GAAAGGACCA 
AGGACCTCCT CTTCGGCAAC AGTCGTTCCA GAGAGTGATG AAGAAGGGCC TTCCCCTGCC 
CCAGATGGGC CCGGGCCACC TTCTGCCTTC AACTTGAACA GCGACACGGA TGAGGAAGAA 
AGTCAGGAAT CAGGAGCAGG GGAGGCCTCT TCAGCTGCCA GAAGAGGTAC CGCTGCAGAG 
ACGGAACAGC CTGAACCTGT CACAGCCGAG ATCCAGATTG AGAAGGATCA GTGTTCCGTG 
AAGGAGAGGA ACGGGGACAC AGAAATCGAG AGGGATGTGA GGAATGGGGT GGTTCCGACT 
GGAGTGATTC TGGAGAGGAG CCAGCCTTCT GGGGAGGACA GTGACACAGA TGTGAGTGAT 
GAGAGTGGGC CTCGAAGAAG GCTTGTTGGA GTCCGTCCGA AAAGGGCCTG GTCTTGTAAC 
TTCATAGACA GTGATACCGA TGGAGAGGAC GAGGGGATCC CTGCTACCCC AGCAGTGGTT 
CCCATGAAGG AGAGGCAGAT CTTCCACGAA GCTGGTACAC AGAGCCCCCA GGCACCTGGT 
GTGGCACGTT GGCAGGAGAG CCCAGCTGAT GGTGATACAG ATATAGAGGA GGGGGAGGCC 
CCCCCAGACA GAAGCCAAGC CTCCGTGGTG ATCGACAGCA ATACAGACGA TGAGGAAGAA 
GTCTCAGCAG CACTGACACT GGCATGTCTA AGAGAGAGCC AGGCTGATTC TGAAGATCTG 
GACCTACCAG CTACCCAGTG CTTTGTAGAC AGAAAGAATC AGAGCCTGGA AGCCCCCAGC 
ATGGAGGATG AGCCCACCCA GGCCTTCCTG TTTCCTCTGC CCCAAGAGCC TGGCCCTTCC 
TGTTGCAGCT CCCAGGCCAC AGGTTCCTTG GATGAGTCAT GGGAGGCCTT GGCGACACAG 
CCATTCTGTC CGAGAGAATC TGAGGCCTCC GAGACCCAAA CCGTTGTCAC CCTCCTTGAC 
ACCCATGCAT CTTGGCCCTC TCCATCTAGG ACAGCACAGC AAGAGCAACA TCCAGAGAGC 
CCAGTCCATG CAGAGCCACT GGGGATGGAA GGCAGAGAGA TGCAGACTGT GGAGAAAGAA 
ATGGGTACCC CAAGAGAAAC AGCAGAGAGG GTGATCCCTG AGGGAGGGCC ACCGCAGGAG 
GAAACCAAGA AACTGCCCTC AGAAGGAGAG AGGGAAGATG TGACGGGAGA GGAAGAATTA 
ATCGGGGGGA TACAGGGCAG AGAAAAAAAT CAGGTGTTTG CTAGAGATAC TCAGAGCCAA 
GAATCTGACA AAAAAGTGAA AAGTGCAAGT ACTGGAAGGG GAATGGAGAT TTTAAAGGTA 
GAAATTGAGA CACCCAAGGA AACACAAGAG AAAGAGAGAG AAAAGCAGAC TCTCGCAGGG 
GAAATATTTG AGAGTGAAGC AGGGAAACTG GTAGTAGAGA GAGAGAGTGA GGCAGATGGG 
TTAGAAGTCA AGGAACCCCA AGAGCTACTG GACAGAAGCC CACAGATGGG GGAGACAGAG 
GCGGGGGACC AGGACCAGAA AGGCCAAGCC TCTGGTCTGC CATCAGAGCC TGGAGCAGGG 
GCAGGAGACC TTCAGGGACT TGCTTCAGAC CCAGTAGCTT CTGGGAGCCA GGCAGGTGGA 
GGAAGGGGAG TCCCAGGGAG CCCCAGGAGG CAGCAGAGAG GTGACTTGAA TTGCGAGATG 
CCACCTGCTC AGAAGGCTTC CAGGGGTGAT CAGGAATCCC CAGATGCTTG TCTGCCTCCT 
GCAGCGCCTG AAGCCTCAGC CGCACTCCCA AATTCCCTCA TCATCTCTCA GATCCAAAAA 
CATCCCGCAC CTCAGTCCCT CCTTTTTCCC TCTCTAGCTC CTTTAGAACT GCCTATTCCC 
AGGACCAGAC AAAATGAGAC TCAGGAAGCT CCAGAGACTC CCTTCTCCTC AGAGCTGAAC 
TCTGTCCACC CAGAACCCAA AGTCAGGCTC CAGGCGTCCT CTCCAGTTTC TTCTCTACCC 
CTTGAGCCCC ACCCTACCAC CCCCACAGGC CAGCCTATTG CCCTTGAACC CATATCTGGG 
GTCTCTTGGA GCAGGACACG TAGTTCCTTT GATGTAACTG CCTCATCAGT TGTCCCCACA 
GCCGTTGCAC TGCAGCCATC CATCTCCACA GACAAGCCTG TCACCCCTAA GCCCACACTT 
CGGGCCCCTC GGGGCAGGGC ACAGAGGTCT TCTGTCAAAA CCCCTGAACC CAATGTCACC 
ACAGACCAGC CTGTTGCCCC TGAGCTCACA GCTATGGCAA CTCGGGGCAG GGCACAGAGG 
TCTTCTGTCA AGATTCCCAA ACCAGATAAC CCCACAACAC CCAAGCCCCA GCCTTCCACT 
TCCACAGACC AGCCTGTCAT CCCCAAACCC ACATCTCGGG CCCCTCGGGG CAGGACACCT 
AGGTCTTCTG CCAAGACTCC TGAACCAGCT GTCCCCACAG CCTCTGAGCT CCAGCCTGCT 
GCCCCTAAAG ACCAGCCTGT TGCTCCTGAG CTCACATCTA GAGCCACTCG GGGCAGGACA 
CAGAGGTCTA TCAAGACTTC CAAACCAGAT ATGCCCACAA CTCCCGAGCC CCAGCCTTCC 
ACCTCCGCAG ACCAGCCTGT CACCCCCAAA CCCACGTCTC GGGCCCCTCG GGGCAGGACA 
CCTAGGTCTT CCGCCAAGAC TCCTGAGCCA GTTGTCTCCA CAGCCTCTGA GCTCCAGCCT 
TCTGCCCTCA CAGATGAGCC TGTCACTCCT GAGCTCACAT CTAGGGCTAC TCGGGGTCGG 
GCACAGAGGT CCTCTGTCAA AACCCCTGAT CCAGTTACCA CCACAACACC TGAGCTCCAG 
CCTTCCACCT CCACAGACCA GCTTGTCACC CCCAAACGCA CATCTCGGGC CCCTCGAGGC 
AGGACACGTA GGTTGTCTGC CAAGACTCCT GAACCAGTTG TTCCCACAGC CTCTGAGCTC 
CAGCCTTCTG CCCCTGCAGA CCAGCCTGTT GGTCCTTGGG CCACTCAGTG TAGAAGACAT 
AGGTCTTCTG TCAAGACCCC AGAACCAGTT GTCCCCACAG TCCCTGAACC TCAGCCTTCC 
ACTTCTAAAG ACCAGTCTGT CACTCCTGAG CCCACATCTC AGGCCACTGA GAGCCAAACA 
CATAGGTCCT CTATCAAGAC ATCCCAGCCA ACGGAACCCA CAGCCTCTGA CCTCAAACCT 
TCCTCCCCCA CAGACCAGCT TGTCACTCCC AAGGTCATAG CTCAGGGTGG TCCAAGCAGG 
ACAGGAAGGG CTTCCACAGC AAGTGCTGTG CTGGTTCCTA CTACCCCTGA ATTCCAGTCT 
CCAGTCCCGT CAGAACAGCC TCTTCCCCCT GACCCCATCC CTGAAGTCAA CTGCAGCAGG 
AGGCTGAGGG CCACTAGGAA ACATGGGTCT CCCACAGCTC ATGTTCATGA GCCCTGCACC 
GTACCCCCTG AACCTAACTC CCACTCTTCA AGGAACCAAA GACATGGGGC AGTGAAAGCA 
GCCAAGCCCC TTAGCACCAT TCCTGAGCCT GCCTTTGCCC AGCTCCCCGA GGCACCGCCT 
CACACTCCCC AGGTGCCAAA GGAGGAGGCA GCAGATGGAT CAGGCTTCAC CCCAGAGCCC 
CAGCCTAGGG CCTCTCAAAA CCACAAGAGG CCTTCAGCTA CTGCACATTC ACCTCCACTT 
CAAAAACGGC TCCAGAGAGG GAGAGTCCCT CAGAAGGCAG CATCCCTTAA GGAAGAAGAA 
AATCCAGTAG CGAGGCCGAG GAAGGAAGAG GGTGTAGTGA TTCCAGGTCC AGGCAAGAGA 
AAGAGAGAGC AGACAGAGGA GGAGTCCCAG GGAAGACCGA GCCGCAGCCT GCGACGGACC 
AGACCGATGC AGGAGTCCAC GGCCCCCAAA GTGCTGTTCA CCGGTGTGGT GGATGCTCGC 
GGAGAGAGGA CGGTGCTGGC CCTGGGGGGC AGTTTGGCCA GCTCGGTGGC CGAGGCTTCT 
CACCTGGTGA CTGATCGGAT CCGCCGGACG GTCAAGTTTC TGTGTGCCCT GGGCCGGGGC 
ATCCCCATCC TCTCCCTGGC CTGGCTGCAT GAGTCCCGCA AGGCAGGCTG CTTCTTGCCC 
CCGGACGAAT ATTTGGTGAC TGATCCTGAG CAGGAGAAGA ACTTCGGCTT CAGCCTTCGG 
GAGGCCCTGA GCCGAGCTCA GGAGCGAAGG CTGCTGGAGG GCTATGAGAT TCATGTGACC 
CCCGGAGTCC AGCCACCGCC ACCTCAGATG GGAGAAATCA TCAGCTGCTG TGGAGGCGCC 
ATCCTGCCCA GCATGCCTCG GTCCTACAAG CCTCAGAGGT TCGTGATCAC ATGTTCCCAG 
GACTTTCCTC GATGTGCTGT TCCATATCGG GTTGGGCTGC CTATCCTCTC ACCCGAATTC 
CTGCTGACGG GAGTACTGAA GCAGGAAGTC AAGCCAGAGG CCTTTGCCTT CTCCACTGTG 
GAAATGTCAT CCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MDC1 ( XM_012164412.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_012019802.1 Ovis aries musimon mediator of DNA damage checkpoint 1 (MDC1), transcript variant X21, mRNA.

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CloneID	OOv35155
Clone ID Related Accession (Same CDS sequence)	XM_012164412.2
Accession Version	XM_012164412.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4968bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	mediator of DNA damage checkpoint protein 1 isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011943095.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012164412.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGTGT CCCACTTTCC TTGTCTACAT TTTCCCTTTA AGATCATGGA AGACACCCAG 
ATTTTAAACT GGGAAGTTGA AGAAGAGGAG GAGGTTGCAG AGAGCCCCAA TGAATCTCTG 
GGGTATAGCT TGGAGCCCCT AGCACAGCTG CATATCTTCA GTAGTTCCTA TGGACCAGAA 
AAAGACTTCC CACTCTACCT TGGAAAGAAC ATGATAGGCC GAATGCCTGA TTGCTCTGTG 
GCCCTGCCCT TTTCATCAAT ATCCAAACAA CATGCAGTGA TTGAAATCTC TGCCTGGGAC 
AAGGCGCCTG TCCTTCGGGA TTGCGGGAGC CTCAATGGCA CTCAAATCCT GCGGCCTCCT 
AAGGTCCTGA GCCCTGGGGT GAGTCATCGT TTGCGGGACC GGGAGTTGAT TCTCTTTGCT 
GACTTGCCCT GCCAGTACCA TCGCTTGGAT GTCCCCCGGC CTTTTGTCTC CCGGGGCCCT 
CTAACTGTAG AGGAGACACC CAGGGTACAG GGAGGAACTC AACCCTCCAG GCTTCTGTTG 
GCTGAGGACT CAGAGGAAGA AGTAGATTCC CTTTTGGACA AGTGTGTGGT GAAAGGACCA 
AGGACCTCCT CTTCGGCAAC AGTCGTTCCA GAGAGTGATG AAGAAGGGCC TTCCCCTGCC 
CCAGATGGGC CCGGGCCACC TTCTGCCTTC AACTTGAACA GCGACACGGA TGAGGAAGAA 
AGTCAGGAAT CAGGAGCAGG GGAGGCCTCT TCAGCTGCCA GAAGAGGTAC CGCTGCAGAG 
ACGGAACAGC CTGAACCTGT CACAGCCGAG ATCCAGATTG AGAAGGATCA GTGTTCCGTG 
AAGGAGAGGA ACGGGGACAC AGAAATCGAG AGGGATGTGA GGAATGGGGT GGTTCCGACT 
GGAGTGATTC TGGAGAGGAG CCAGCCTTCT GGGGAGGACA GTGACACAGA TGTGAGTGAT 
GAGAGTGGGC CTCGAAGAAG GCTTGTTGGA GTCCGTCCGA AAAGGGCCTG GTCTTGTAAC 
TTCATAGACA GTGATACCGA TGGAGAGGAC GAGGGGATCC CTGCTACCCC AGCAGTGGTT 
CCCATGAAGG AGAGGCAGAT CTTCCACGAA GCTGGTACAC AGAGCCCCCA GGCACCTGGT 
GTGGCACGTT GGCAGGAGAG CCCAGCTGAT GGTGATACAG ATATAGAGGA GGGGGAGGCC 
CCCCCAGACA GAAGCCAAGC CTCCGTGGTG ATCGACAGCA ATACAGACGA TGAGGAAGAA 
GTCTCAGCAG CACTGACACT GGCATGTCTA AGAGAGAGCC AGGCTGATTC TGAAGATCTG 
GACCTACCAG CTACCCAGTG CTTTGTAGAC AGAAAGAATC AGAGCCTGGA AGGTTCCTTG 
GATGAGTCAT GGGAGGCCTT GGCGACACAG CCATTCTGTC CGAGAGAATC TGAGGCCTCC 
GAGACCCAAA CCGTTGTCAC CCTCCTTGAC ACCCATGCAT CTTGGCCCTC TCCATCTAGG 
ACAGCACAGC AAGAGCAACA TCCAGAGAGC CCAGTCCATG CAGAGCCACT GGGGATGGAA 
GGCAGAGAGA TGCAGACTGT GGAGAAAGAA ATGGGTACCC CAAGAGAAAC AGCAGAGAGG 
GTGATCCCTG AGGGAGGGCC ACCGCAGGAG GAAACCAAGA AACTGCCCTC AGAAGGAGAG 
AGGGAAGATG TGACGGGAGA GGAAGAATTA ATCGGGGGGA TACAGGGCAG AGAAAAAAAT 
CAGGTGTTTG CTAGAGATAC TCAGAGCCAA GAATCTGACA AAAAAGTGAA AAGTGCAAGT 
ACTGGAAGGG GAATGGAGAT TTTAAAGGTA GAAATTGAGA CACCCAAGGA AACACAAGAG 
AAAGAGAGAG AAAAGCAGAC TCTCGCAGGG GAAATATTTG AGAGTGAAGC AGGGAAACTG 
GTAGTAGAGA GAGAGAGTGA GGCAGATGGG TTAGAAGTCA AGGAACCCCA AGAGCTACTG 
GACAGAAGCC CACAGATGGG GGAGACAGAG GCGGGGGACC AGGACCAGAA AGGCCAAGCC 
TCTGGTCTGC CATCAGAGCC TGGAGCAGGG GCAGGAGACC TTCAGGGACT TGCTTCAGAC 
CCAGTAGCTT CTGGGAGCCA GGCAGGTGGA GGAAGGGGAG TCCCAGGGAG CCCCAGGAGG 
CAGCAGAGAG GTGACTTGAA TTGCGAGATG CCACCTGCTC AGAAGGCTTC CAGGGGTGAT 
CAGGAATCCC CAGATGCTTG TCTGCCTCCT GCAGCGCCTG AAGCCTCAGC CGCACTCCCA 
AATTCCCTCA TCATCTCTCA GATCCAAAAA CATCCCGCAC CTCAGTCCCT CCTTTTTCCC 
TCTCTAGCTC CTTTAGAACT GCCTATTCCC AGGACCAGAC AAAATGAGAC TCAGGAAGCT 
CCAGAGACTC CCTTCTCCTC AGAGCTGAAC TCTGTCCACC CAGAACCCAA AGTCAGGCTC 
CAGGCGTCCT CTCCAGTTTC TTCTCTACCC CTTGAGCCCC ACCCTACCAC CCCCACAGGC 
CAGCCTATTG CCCTTGAACC CATATCTGGG GTCTCTTGGA GCAGGACACG TAGTTCCTTT 
GATGTAACTG CCTCATCAGT TGTCCCCACA GCCGTTGCAC TGCAGCCATC CATCTCCACA 
GACAAGCCTG TCACCCCTAA GCCCACACTT CGGGCCCCTC GGGGCAGGGC ACAGAGGTCT 
TCTGTCAAAA CCCCTGAACC CAATGTCACC ACAGACCAGC CTGTTGCCCC TGAGCTCACA 
GCTATGGCAA CTCGGGGCAG GGCACAGAGG TCTTCTGTCA AGATTCCCAA ACCAGATAAC 
CCCACAACAC CCAAGCCCCA GCCTTCCACT TCCACAGACC AGCCTGTCAT CCCCAAACCC 
ACATCTCGGG CCCCTCGGGG CAGGACACCT AGGTCTTCTG CCAAGACTCC TGAACCAGCT 
GTCCCCACAG CCTCTGAGCT CCAGCCTGCT GCCCCTAAAG ACCAGCCTGT TGCTCCTGAG 
CTCACATCTA GAGCCACTCG GGGCAGGACA CAGAGGTCTA TCAAGACTTC CAAACCAGAT 
ATGCCCACAA CTCCCGAGCC CCAGCCTTCC ACCTCCGCAG ACCAGCCTGT CACCCCCAAA 
CCCACGTCTC GGGCCCCTCG GGGCAGGACA CCTAGGTCTT CCGCCAAGAC TCCTGAGCCA 
GTTGTCTCCA CAGCCTCTGA GCTCCAGCCT TCTGCCCTCA CAGATGAGCC TGTCACTCCT 
GAGCTCACAT CTAGGGCTAC TCGGGGTCGG GCACAGAGGT CCTCTGTCAA AACCCCTGAT 
CCAGTTACCA CCACAACACC TGAGCTCCAG CCTTCCACCT CCACAGACCA GCTTGTCACC 
CCCAAACGCA CATCTCGGGC CCCTCGAGGC AGGACACGTA GGTTGTCTGC CAAGACTCCT 
GAACCAGTTG TTCCCACAGC CTCTGAGCTC CAGCCTTCTG CCCCTGCAGA CCAGCCTGTT 
GGTCCTTGGG CCACTCAGTG TAGAAGACAT AGGTCTTCTG TCAAGACCCC AGAACCAGTT 
GTCCCCACAG TCCCTGAACC TCAGCCTTCC ACTTCTAAAG ACCAGTCTGT CACTCCTGAG 
CCCACATCTC AGGCCACTGA GAGCCAAACA CATAGGTCCT CTATCAAGAC ATCCCAGCCA 
ACGGAACCCA CAGCCTCTGA CCTCAAACCT TCCTCCCCCA CAGACCAGCT TGTCACTCCC 
AAGGTCATAG CTCAGGGTGG TCCAAGCAGG ACAGGAAGGG CTTCCACAGC AAGTGCTGTG 
CTGGTTCCTA CTACCCCTGA ATTCCAGTCT CCAGTCCCGT CAGAACAGCC TCTTCCCCCT 
GACCCCATCC CTGAAGTCAA CTGCAGCAGG AGGCTGAGGG CCACTAGGAA ACATGGGTCT 
CCCACAGCTC ATGTTCATGA GCCCTGCACC GTACCCCCTG AACCTAACTC CCACTCTTCA 
AGGAACCAAA GACATGGGGC AGTGAAAGCA GCCAAGCCCC TTAGCACCAT TCCTGAGCCT 
GCCTTTGCCC AGCTCCCCGA GGCACCGCCT CACACTCCCC AGGTGCCAAA GGAGGAGGCA 
GCAGATGGAT CAGGCTTCAC CCCAGAGCCC CAGCCTAGGG CCTCTCAAAA CCACAAGAGG 
CCTTCAGCTA CTGCACATTC ACCTCCACTT CAAAAACGGC TCCAGAGAGG GAGAGTCCCT 
CAGAAGGCAG CATCCCTTAA GGAAGAAGAA AATCCAGTAG CGAGGCCGAG GAAGGAAGAG 
GGTGTAGTGA TTCCAGGTCC AGGCAAGAGA AAGAGAGAGC AGACAGAGGA GGAGTCCCAG 
GGAAGACCGA GCCGCAGCCT GCGACGGACC AGACCGATGC AGGAGTCCAC GGCCCCCAAA 
GTGCTGTTCA CCGGTGTGGT GGATGCTCGC GGAGAGAGGA CGGTGCTGGC CCTGGGGGGC 
AGTTTGGCCA GCTCGGTGGC CGAGGCTTCT CACCTGGTGA CTGATCGGAT CCGCCGGACG 
GTCAAGTTTC TGTGTGCCCT GGGCCGGGGC ATCCCCATCC TCTCCCTGGC CTGGCTGCAT 
GAGTCCCGCA AGGCAGGCTG CTTCTTGCCC CCGGACGAAT ATTTGGTGAC TGATCCTGAG 
CAGGAGAAGA ACTTCGGCTT CAGCCTTCGG GAGGCCCTGA GCCGAGCTCA GGAGCGAAGG 
CTGCTGGAGG GCTATGAGAT TCATGTGACC CCCGGAGTCC AGCCACCGCC ACCTCAGATG 
GGAGAAATCA TCAGCTGCTG TGGAGGCGCC ATCCTGCCCA GCATGCCTCG GTCCTACAAG 
CCTCAGAGGT TCGTGATCAC ATGTTCCCAG GACTTTCCTC GATGTGCTGT TCCATATCGG 
GTTGGGCTGC CTATCCTCTC ACCCGAATTC CTGCTGACGG GAGTACTGAA GCAGGAAGTC 
AAGCCAGAGG CCTTTGCCTT CTCCACTGTG GAAATGTCAT CCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012019802.1
CDS	328..5295
Translation	MEVSHFPCLHFPFKIMEDTQILNWEVEEEEEVAESPNESLGYSLEPLAQLHIFSSSYGPEKDFPLYLGKNMIGRMPDCSVALPFSSISKQHAVIEISAWDKAPVLRDCGSLNGTQILRPPKVLSPGVSHRLRDRELILFADLPCQYHRLDVPRPFVSRGPLTVEETPRVQGGTQPSRLLLAEDSEEEVDSLLDKCVVKGPRTSSSATVVPESDEEGPSPAPDGPGPPSAFNLNSDTDEEESQESGAGEASSAARRGTAAETEQPEPVTAEIQIEKDQCSVKERNGDTEIERDVRNGVVPTGVILERSQPSGEDSDTDVSDESGPRRRLVGVRPKRAWSCNFIDSDTDGEDEGIPATPAVVPMKERQIFHEAGTQSPQAPGVARWQESPADGDTDIEEGEAPPDRSQASVVIDSNTDDEEEVSAALTLACLRESQADSEDLDLPATQCFVDRKNQSLEGSLDESWEALATQPFCPRESEASETQTVVTLLDTHASWPSPSRTAQQEQHPESPVHAEPLGMEGREMQTVEKEMGTPRETAERVIPEGGPPQEETKKLPSEGEREDVTGEEELIGGIQGREKNQVFARDTQSQESDKKVKSASTGRGMEILKVEIETPKETQEKEREKQTLAGEIFESEAGKLVVERESEADGLEVKEPQELLDRSPQMGETEAGDQDQKGQASGLPSEPGAGAGDLQGLASDPVASGSQAGGGRGVPGSPRRQQRGDLNCEMPPAQKASRGDQESPDACLPPAAPEASAALPNSLIISQIQKHPAPQSLLFPSLAPLELPIPRTRQNETQEAPETPFSSELNSVHPEPKVRLQASSPVSSLPLEPHPTTPTGQPIALEPISGVSWSRTRSSFDVTASSVVPTAVALQPSISTDKPVTPKPTLRAPRGRAQRSSVKTPEPNVTTDQPVAPELTAMATRGRAQRSSVKIPKPDNPTTPKPQPSTSTDQPVIPKPTSRAPRGRTPRSSAKTPEPAVPTASELQPAAPKDQPVAPELTSRATRGRTQRSIKTSKPDMPTTPEPQPSTSADQPVTPKPTSRAPRGRTPRSSAKTPEPVVSTASELQPSALTDEPVTPELTSRATRGRAQRSSVKTPDPVTTTTPELQPSTSTDQLVTPKRTSRAPRGRTRRLSAKTPEPVVPTASELQPSAPADQPVGPWATQCRRHRSSVKTPEPVVPTVPEPQPSTSKDQSVTPEPTSQATESQTHRSSIKTSQPTEPTASDLKPSSPTDQLVTPKVIAQGGPSRTGRASTASAVLVPTTPEFQSPVPSEQPLPPDPIPEVNCSRRLRATRKHGSPTAHVHEPCTVPPEPNSHSSRNQRHGAVKAAKPLSTIPEPAFAQLPEAPPHTPQVPKEEAADGSGFTPEPQPRASQNHKRPSATAHSPPLQKRLQRGRVPQKAASLKEEENPVARPRKEEGVVIPGPGKRKREQTEEESQGRPSRSLRRTRPMQESTAPKVLFTGVVDARGERTVLALGGSLASSVAEASHLVTDRIRRTVKFLCALGRGIPILSLAWLHESRKAGCFLPPDEYLVTDPEQEKNFGFSLREALSRAQERRLLEGYEIHVTPGVQPPPPQMGEIISCCGGAILPSMPRSYKPQRFVITCSQDFPRCAVPYRVGLPILSPEFLLTGVLKQEVKPEAFAFSTVEMSST

Target ORF information:

RefSeq Version	XM_012164412.2
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon mediator of DNA damage checkpoint 1 (MDC1), transcript variant X21, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012164412.2

ORF Insert Sequence:

ATGGAGGTGT CCCACTTTCC TTGTCTACAT TTTCCCTTTA AGATCATGGA AGACACCCAG 
ATTTTAAACT GGGAAGTTGA AGAAGAGGAG GAGGTTGCAG AGAGCCCCAA TGAATCTCTG 
GGGTATAGCT TGGAGCCCCT AGCACAGCTG CATATCTTCA GTAGTTCCTA TGGACCAGAA 
AAAGACTTCC CACTCTACCT TGGAAAGAAC ATGATAGGCC GAATGCCTGA TTGCTCTGTG 
GCCCTGCCCT TTTCATCAAT ATCCAAACAA CATGCAGTGA TTGAAATCTC TGCCTGGGAC 
AAGGCGCCTG TCCTTCGGGA TTGCGGGAGC CTCAATGGCA CTCAAATCCT GCGGCCTCCT 
AAGGTCCTGA GCCCTGGGGT GAGTCATCGT TTGCGGGACC GGGAGTTGAT TCTCTTTGCT 
GACTTGCCCT GCCAGTACCA TCGCTTGGAT GTCCCCCGGC CTTTTGTCTC CCGGGGCCCT 
CTAACTGTAG AGGAGACACC CAGGGTACAG GGAGGAACTC AACCCTCCAG GCTTCTGTTG 
GCTGAGGACT CAGAGGAAGA AGTAGATTCC CTTTTGGACA AGTGTGTGGT GAAAGGACCA 
AGGACCTCCT CTTCGGCAAC AGTCGTTCCA GAGAGTGATG AAGAAGGGCC TTCCCCTGCC 
CCAGATGGGC CCGGGCCACC TTCTGCCTTC AACTTGAACA GCGACACGGA TGAGGAAGAA 
AGTCAGGAAT CAGGAGCAGG GGAGGCCTCT TCAGCTGCCA GAAGAGGTAC CGCTGCAGAG 
ACGGAACAGC CTGAACCTGT CACAGCCGAG ATCCAGATTG AGAAGGATCA GTGTTCCGTG 
AAGGAGAGGA ACGGGGACAC AGAAATCGAG AGGGATGTGA GGAATGGGGT GGTTCCGACT 
GGAGTGATTC TGGAGAGGAG CCAGCCTTCT GGGGAGGACA GTGACACAGA TGTGAGTGAT 
GAGAGTGGGC CTCGAAGAAG GCTTGTTGGA GTCCGTCCGA AAAGGGCCTG GTCTTGTAAC 
TTCATAGACA GTGATACCGA TGGAGAGGAC GAGGGGATCC CTGCTACCCC AGCAGTGGTT 
CCCATGAAGG AGAGGCAGAT CTTCCACGAA GCTGGTACAC AGAGCCCCCA GGCACCTGGT 
GTGGCACGTT GGCAGGAGAG CCCAGCTGAT GGTGATACAG ATATAGAGGA GGGGGAGGCC 
CCCCCAGACA GAAGCCAAGC CTCCGTGGTG ATCGACAGCA ATACAGACGA TGAGGAAGAA 
GTCTCAGCAG CACTGACACT GGCATGTCTA AGAGAGAGCC AGGCTGATTC TGAAGATCTG 
GACCTACCAG CTACCCAGTG CTTTGTAGAC AGAAAGAATC AGAGCCTGGA AGGTTCCTTG 
GATGAGTCAT GGGAGGCCTT GGCGACACAG CCATTCTGTC CGAGAGAATC TGAGGCCTCC 
GAGACCCAAA CCGTTGTCAC CCTCCTTGAC ACCCATGCAT CTTGGCCCTC TCCATCTAGG 
ACAGCACAGC AAGAGCAACA TCCAGAGAGC CCAGTCCATG CAGAGCCACT GGGGATGGAA 
GGCAGAGAGA TGCAGACTGT GGAGAAAGAA ATGGGTACCC CAAGAGAAAC AGCAGAGAGG 
GTGATCCCTG AGGGAGGGCC ACCGCAGGAG GAAACCAAGA AACTGCCCTC AGAAGGAGAG 
AGGGAAGATG TGACGGGAGA GGAAGAATTA ATCGGGGGGA TACAGGGCAG AGAAAAAAAT 
CAGGTGTTTG CTAGAGATAC TCAGAGCCAA GAATCTGACA AAAAAGTGAA AAGTGCAAGT 
ACTGGAAGGG GAATGGAGAT TTTAAAGGTA GAAATTGAGA CACCCAAGGA AACACAAGAG 
AAAGAGAGAG AAAAGCAGAC TCTCGCAGGG GAAATATTTG AGAGTGAAGC AGGGAAACTG 
GTAGTAGAGA GAGAGAGTGA GGCAGATGGG TTAGAAGTCA AGGAACCCCA AGAGCTACTG 
GACAGAAGCC CACAGATGGG GGAGACAGAG GCGGGGGACC AGGACCAGAA AGGCCAAGCC 
TCTGGTCTGC CATCAGAGCC TGGAGCAGGG GCAGGAGACC TTCAGGGACT TGCTTCAGAC 
CCAGTAGCTT CTGGGAGCCA GGCAGGTGGA GGAAGGGGAG TCCCAGGGAG CCCCAGGAGG 
CAGCAGAGAG GTGACTTGAA TTGCGAGATG CCACCTGCTC AGAAGGCTTC CAGGGGTGAT 
CAGGAATCCC CAGATGCTTG TCTGCCTCCT GCAGCGCCTG AAGCCTCAGC CGCACTCCCA 
AATTCCCTCA TCATCTCTCA GATCCAAAAA CATCCCGCAC CTCAGTCCCT CCTTTTTCCC 
TCTCTAGCTC CTTTAGAACT GCCTATTCCC AGGACCAGAC AAAATGAGAC TCAGGAAGCT 
CCAGAGACTC CCTTCTCCTC AGAGCTGAAC TCTGTCCACC CAGAACCCAA AGTCAGGCTC 
CAGGCGTCCT CTCCAGTTTC TTCTCTACCC CTTGAGCCCC ACCCTACCAC CCCCACAGGC 
CAGCCTATTG CCCTTGAACC CATATCTGGG GTCTCTTGGA GCAGGACACG TAGTTCCTTT 
GATGTAACTG CCTCATCAGT TGTCCCCACA GCCGTTGCAC TGCAGCCATC CATCTCCACA 
GACAAGCCTG TCACCCCTAA GCCCACACTT CGGGCCCCTC GGGGCAGGGC ACAGAGGTCT 
TCTGTCAAAA CCCCTGAACC CAATGTCACC ACAGACCAGC CTGTTGCCCC TGAGCTCACA 
GCTATGGCAA CTCGGGGCAG GGCACAGAGG TCTTCTGTCA AGATTCCCAA ACCAGATAAC 
CCCACAACAC CCAAGCCCCA GCCTTCCACT TCCACAGACC AGCCTGTCAT CCCCAAACCC 
ACATCTCGGG CCCCTCGGGG CAGGACACCT AGGTCTTCTG CCAAGACTCC TGAACCAGCT 
GTCCCCACAG CCTCTGAGCT CCAGCCTGCT GCCCCTAAAG ACCAGCCTGT TGCTCCTGAG 
CTCACATCTA GAGCCACTCG GGGCAGGACA CAGAGGTCTA TCAAGACTTC CAAACCAGAT 
ATGCCCACAA CTCCCGAGCC CCAGCCTTCC ACCTCCGCAG ACCAGCCTGT CACCCCCAAA 
CCCACGTCTC GGGCCCCTCG GGGCAGGACA CCTAGGTCTT CCGCCAAGAC TCCTGAGCCA 
GTTGTCTCCA CAGCCTCTGA GCTCCAGCCT TCTGCCCTCA CAGATGAGCC TGTCACTCCT 
GAGCTCACAT CTAGGGCTAC TCGGGGTCGG GCACAGAGGT CCTCTGTCAA AACCCCTGAT 
CCAGTTACCA CCACAACACC TGAGCTCCAG CCTTCCACCT CCACAGACCA GCTTGTCACC 
CCCAAACGCA CATCTCGGGC CCCTCGAGGC AGGACACGTA GGTTGTCTGC CAAGACTCCT 
GAACCAGTTG TTCCCACAGC CTCTGAGCTC CAGCCTTCTG CCCCTGCAGA CCAGCCTGTT 
GGTCCTTGGG CCACTCAGTG TAGAAGACAT AGGTCTTCTG TCAAGACCCC AGAACCAGTT 
GTCCCCACAG TCCCTGAACC TCAGCCTTCC ACTTCTAAAG ACCAGTCTGT CACTCCTGAG 
CCCACATCTC AGGCCACTGA GAGCCAAACA CATAGGTCCT CTATCAAGAC ATCCCAGCCA 
ACGGAACCCA CAGCCTCTGA CCTCAAACCT TCCTCCCCCA CAGACCAGCT TGTCACTCCC 
AAGGTCATAG CTCAGGGTGG TCCAAGCAGG ACAGGAAGGG CTTCCACAGC AAGTGCTGTG 
CTGGTTCCTA CTACCCCTGA ATTCCAGTCT CCAGTCCCGT CAGAACAGCC TCTTCCCCCT 
GACCCCATCC CTGAAGTCAA CTGCAGCAGG AGGCTGAGGG CCACTAGGAA ACATGGGTCT 
CCCACAGCTC ATGTTCATGA GCCCTGCACC GTACCCCCTG AACCTAACTC CCACTCTTCA 
AGGAACCAAA GACATGGGGC AGTGAAAGCA GCCAAGCCCC TTAGCACCAT TCCTGAGCCT 
GCCTTTGCCC AGCTCCCCGA GGCACCGCCT CACACTCCCC AGGTGCCAAA GGAGGAGGCA 
GCAGATGGAT CAGGCTTCAC CCCAGAGCCC CAGCCTAGGG CCTCTCAAAA CCACAAGAGG 
CCTTCAGCTA CTGCACATTC ACCTCCACTT CAAAAACGGC TCCAGAGAGG GAGAGTCCCT 
CAGAAGGCAG CATCCCTTAA GGAAGAAGAA AATCCAGTAG CGAGGCCGAG GAAGGAAGAG 
GGTGTAGTGA TTCCAGGTCC AGGCAAGAGA AAGAGAGAGC AGACAGAGGA GGAGTCCCAG 
GGAAGACCGA GCCGCAGCCT GCGACGGACC AGACCGATGC AGGAGTCCAC GGCCCCCAAA 
GTGCTGTTCA CCGGTGTGGT GGATGCTCGC GGAGAGAGGA CGGTGCTGGC CCTGGGGGGC 
AGTTTGGCCA GCTCGGTGGC CGAGGCTTCT CACCTGGTGA CTGATCGGAT CCGCCGGACG 
GTCAAGTTTC TGTGTGCCCT GGGCCGGGGC ATCCCCATCC TCTCCCTGGC CTGGCTGCAT 
GAGTCCCGCA AGGCAGGCTG CTTCTTGCCC CCGGACGAAT ATTTGGTGAC TGATCCTGAG 
CAGGAGAAGA ACTTCGGCTT CAGCCTTCGG GAGGCCCTGA GCCGAGCTCA GGAGCGAAGG 
CTGCTGGAGG GCTATGAGAT TCATGTGACC CCCGGAGTCC AGCCACCGCC ACCTCAGATG 
GGAGAAATCA TCAGCTGCTG TGGAGGCGCC ATCCTGCCCA GCATGCCTCG GTCCTACAAG 
CCTCAGAGGT TCGTGATCAC ATGTTCCCAG GACTTTCCTC GATGTGCTGT TCCATATCGG 
GTTGGGCTGC CTATCCTCTC ACCCGAATTC CTGCTGACGG GAGTACTGAA GCAGGAAGTC 
AAGCCAGAGG CCTTTGCCTT CTCCACTGTG GAAATGTCAT CCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MDC1 ( XM_012100984.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_011956374.1 Ovis aries mediator of DNA damage checkpoint 1 (MDC1), transcript variant X1, mRNA.

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CloneID	OSm28025
Clone ID Related Accession (Same CDS sequence)	XM_012100984.2
Accession Version	XM_012100984.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5811bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries(sheep)
Product	mediator of DNA damage checkpoint protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014639029.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012100984.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGTGT CCCACTTTCC TTGTCTACAT TTTCCCTTTA AGATCATGGA AGACACCCAG 
ATTTTAAACT GGGAAGTTGA AGAAGAGGAG GAGGTTGCAG AGAGCCCCAA TGAATCTCTG 
GGGTATAGCT TGGAGCCCCT AGCACAGCTG CATATCTTCA GTAGTTCCTA TGGACCAGAA 
AAAGACTTTC CACTCTACCT TGGAAAGAAC ATGATAGGCC GAATGCCTGA TTGCTCTGTG 
GCCCTGCCCT TTTCATCAAT ATCCAAACAA CATGCAGTGA TTGAAATCTC TGCCTGGGAC 
AAGGCGCCTG TCCTTCGGGA TTGCGGGAGC CTCAATGGCA CTCAAATCCT GCGGCCTCCT 
AAGGTCCTGA GCCCTGGGGT GAGTCATCGT TTGCGGGACC GGGAGTTGAT TCTCTTTGCT 
GACTTGCCCT GCCAGTACCA TCGCTTGGAT GTCCCCCGGC CTTTTGTCTC CCGGGGCCCT 
CTAAGTGTAG AGGAGACACC CAGGGTACAG GGAGGAACTC AACCCTCCAG GCTTCTGTTG 
GCTGAGGACT CAGAGGAAGA AGTAGATTCC CTTTTGGACA AGTGTGTGGT GAAAGGACCA 
AGGACCTCCT CTTCGGCAAC AGTCGTTCCA GAGAGTGATG AAGAAGGGCC TTCCCCTGCC 
CCAGATGGGC CCGGGCCACC TTCTGCCTTC AACTTGAACA GCGACACGGA TGAGGAAGAA 
AGTCAGGAAT CAGGAGCAGG GGAGGCCTCT TCAGCTGCCA GAAGAGGTAC CGCTGCAGAG 
ACGGAACAGC CTGAACCTGT CACAGCCGAG ATCCAGATTG AGAAGGATCA GTGTTCCGTG 
AAGGAGAGGA ACGGGGACAC AGAAATCGAG AGGGATGTGA GGAATGGGGT GGTTCCGACT 
GGAGTGATTC TGGAGAGGAG CCAGCCTTCT GGGGAGGACA GTGACACAGA TGTGAGTGAT 
GAGAGTGGGC CTCGAAGAAG GCTGGTTGGA GTCCGTCCGA AAAGGGCCTG GTCTTGTAAC 
TTCATAGACA GTGATACCGA TGGAGAGGAC GAGGGGATCC CTGCTACCCC AGCAGTGGTT 
CCCATGAAGG AGAGGCAGAT CTTCCACGAA GCTGGTACAC AGAGCCCCCA GGCACCTGGT 
GTGGCACGTT GGCAGGAGAG CCCAGCTGAT GGTGATACAG ATATAGAGGA GGGGGAGGCC 
CCCCCAGACA GAAGCCAAGC CTCCGTGGTG ATCGACAGCA ATACAGACGA TGAGGAAGAA 
GTCTCAGCAG CACTGACACT GGCATGTCTA AGAGAGAGCC AGGCTGGTAA GTGGAACCGA 
GATCCAGATG CAGAAGAGAC CAGGGCCCAA CCCGTGGCCC TTCTGGAGCG AAGCCAGGCC 
TCTGCTGGGG GAGACAGTGA CACAGATGTG GAGGAAGAGG GGCTCCCAGT GGAAAGGAGG 
GGAATGGTTC CCAAGGGTTA CATGGACAGG GAATATTCAA AAAAGAGCCA GCACCCTCCC 
AGGGACAGTG ATACAGAGGG GAAGGAAGAT GAGAGCTCAC CGGGGGTCCA CCTGGAGAGA 
AGCCAGGCCT CTGCACAAGT GGAGGATGAG GTCCCACTGG GGCCAGCTGT TGCACTTCCG 
GAGAAGCGTC AGGTACAAGG CATAGTGTGG CCACATCACA CCGATGCGGA GGCAGAAGGG 
GACCCGGCAC AGCTCCCCGT GCTGCGTCTA GAGGAAGCCC GGCCTCCTCT AGCTGGGGAC 
TGTGAACTGG ATTCGGAGAA CACATCTGCT GTCAGAAAGA GCCAGCTTCC AGCGGAAAAA 
GATGCTGGGA CCACGTGGGC TGCAGCCGTT CCTGAACAGG ACAGAGCACT TGCCATCGGG 
ACCCAGGGTG GGTCATCCAC GGCACCAGGG GAGCAAGACC TTCTCCCGGT CTCAAGGGAA 
GACCTAGCAG ATATGGTGGT GGACATAGGC ACTCCAGGGG AGCCCCAACC GCAGAGAGAG 
GGGGCCCAGA CCACCACAGG AAGGGAGAGA GAACCATATG GGAATAGGGC CACAGACTCT 
GGAGACAACC TCCACGATTC TGAAGATCTG GACCTACCAG CTACCCAGTG CTTTGTAGAC 
AGAAAGAATC AGAGCCTGGA AGCAGCCCCC AGCATGGAGG ATGAGCCCAC CCAGGCCTTC 
CTGTTTCCTC TGCCCCAAGA GCCTGGCCCT TCCTGTTGCA GCTCCCAGGC CACAGGTTCC 
TTGGATGAGT CATGGGAGGC CTTGGCGACA CAGCCATTCT GTCCGAGAGA ATCTGAGGCC 
TCCGAGACCC AAACCGTTGT CACCCTCCTT GACACCCATG CATCTTGGCC CTCTCCATCT 
AGGACAGCAC AGCAAGAGCA ACATCCAGAG AGCCCAGTCC ATGCAGAGCC ACTGGGGATG 
GAAGGCAGAG AGATGCAGAC TGTGGAGAAA GAAATGGGTA CCCCAAGAGA AACAGCAGAG 
AGGGTGATCC CTGAGGGAGG GCCACCGCAG GAGGAAACCA AGAAACTGCC CTCAGAAGGA 
GAGAGGGAAG ATGTGACGGG AGAGGAAGAA TTAATCCGGG GGATACAGGG CAGTGAAAAA 
AATCAGGTGT TTGCTAGAGA TACTCAGAGC CAAGAATCTG ACAAAAAAGT GAAAAGTGCA 
AGTACTGGAA GGGGAATGGA GATTTTAAAG GTAGAAATTG AGACACCCAA GGAAACACAA 
GAGAAAGAGA GAGAAAAGCA GACTCTCGCA GGGGAAATAT TTGAGAGTGA AGCAGGGAAA 
CTGGTAGTAG AGAGAGAGAG TGAGGCAGAT GGGTTAGAAG TCAAGGAACC CCAAGAGCTA 
CTGGACAGAA GCCCACAGAT GGGGGAGACA GAGGCGGGGG ACCAGGACCA GAAAGGCCAA 
GCCTCTGGTC TGCCATCAGA GCCTGGAGCA GGGGCAGGAG ACCTTCAGGG ACTTGCTTCA 
GACCCAGTAG CTTCTGGGAG CCAGGCAGGT GGAGGAAGGG GAGCCCCAGG GAGCCCCAGG 
AGGCAGCAGA GAGGTGACTT GAATTGCGAG ATGCCACCTG CTCAGAAGGC TTCCAGGGGT 
GATCAGGAAT CCCCAGATGC TTGTCTGCCT CCTGCAGCGC CTGAAGCCTC AGCCGCACTC 
CCAAATTCCC TCATCATCTC TCAAATCCAA AAACATCCCG CACCTCAGTC CCTCCTTTTT 
CCCTCTCTAG CTCCTTTAGA ACTGCCTATT CCCAGGACCA GACAAAATGA GAGTCAGGAA 
GCTCCAGAGA CTCCCTTCTC CTCAGAGCTG AACTCTGTCC ACCCAGAACC CAAAGTCAGG 
CTCCAGGCGT CCTCTCCAGT TTCTTCTCTA CCCCTTGAGC CCCACCCTAC CACCCCCACA 
GGCCAGCCTA TTGCCCTTGA ACCCATATCT GGGGTCTCTC GGAGCAGGAC ACGTAGTTCC 
TTTGATGTAA CTGCCTCATC AGTTGTCCCC ACAGCCGTTG CACTGCAGCC ATCCACCTCC 
ACAGACAAGC CTGTCACCCC TAAGCCCACA CTTCGGGCCC CTCGGGGCAG GGCACAGAGG 
TCTTCTGTCA AAACCCCTGA ACCCAATGTC ACCACAGACC AGCCTGTTGC CCCTGAGCTC 
ACAGCTATGG CAACTCGGGG CAGGGCACAG AGGTCTTCTG TCAAGATTCC CAAACCAGAT 
AACCCCACAA CACCCAAGCC CCAGCCTTCC ACTTCCACAG ACCAGCCTGT CATCCCCAAA 
CCCACATCTC GGGCCCCTCG GGGCAGGACA CCTAGGTCTT CTGCCAAGAC TCCTGAACCA 
GCTGTCCCCA CAGCCTCTGA GCTCCAGCCT GCTGCCCCTA AAGACCAGCC TGTTGCTCCT 
GAGCTCACAT CTAGAGCCAC TCGGGGCAAG ACACAGAGGT CTATCAAGAC TTCCAAACCA 
GATATGCCCA CAACTCCCGA GCCCCAGCCT TCCACCTCCA CAGACCAGCC TGTCACCCCC 
AAACCCACGT CTCGGGCCCC TCGGGGCAGG ACACCTAGGT CTTCCGCCAA GACTCCTGAG 
CCAGTTGTCT CCACAGCCTC TGAGCTCCAG CCTTCTGCCC TCACAGATGA GCCTGTCACT 
CCTGAGCTCA CATCTAGGGC TACTCGGGGT CGGGCACAGA GGTCCTCTGT CAAAACCCCT 
GATCCAGTTA CCACCACAAC ACCTGAGCTC CAGCCTTCCA CCTCCACAGA CCAGCTTGTC 
ACCCCCAAAC GCACATCTCG GGCCCCTCGA GGCAGGACAC GTAGGTTGTC TGCCAAGACT 
CCTGAACCAG TTGTTCCCAC AGCCTCTGAG CTCCAGCCTT CTGCCCCTGC AGACCAGCCT 
GTTGGTCCTT GGGCCACTCA GTGTAGAAGA CATAGGTCTT CTGTCAAGAC CCCAGAACCA 
GTTGTCCCCA CAGTCCCTGA ACCTCAGCCT TCCACTTCTA AAGACCAGTC TGTCACTCCT 
GAGCCCACAT CTCAGGCCAC TGAGAGCCAA ACACATAGGT CCTCTATCAA GACATCCCAG 
CCAACGGAAC CCACAGCCTC TGACCTCAAA CCTTCCTCCC CCACAGACCA GCTTGTCACT 
CCCAAGGTCA TAGCTCAGGG TGGTCCAAGC AGGACAGGAA AGGCTTCCAC AGCAAGTGCT 
GTGCTGGTTC CTACTACCCC TGAATTCCAG TCTCCAGTCC CGTCAGAACA GCCTCTTCCC 
CCTGACCCCA TCCCTGAAGT CAACTGCAGC AGGAGGCTGA GGGCCACTAG GAAACATGGG 
TCTCCCACAG CTCATGTTCA TGAGCCCTGC ACCGTACCCC CTGAACCTAA CTCCCACTCT 
TCAAGGAACC AAAGACATGG GGCAGTGAAA GCAGCCAAGC CCCTTAGCAC CATTCCTGAG 
CCTGCCTTTG CCCAGCTCCC CGAGGCACCG CCTCACACTC CCCAGGTGCC AAAGGAGGAG 
GCAGCAGATG GATCAGGCTT CACCCCAGAG CCCCAGCCTA GGGCCTCTCA AAACCACAAG 
AGGCCTTCAG CTACTGCACA TTCACCTCCA CTTCAAAAAC GGCTCCAGAG AGGGAGAGTC 
CCTCAGAAGG CAGCATCCCT TAAGGAAGAA GAAAATCCAG TAGCGAGGCC GAGGAAGGAA 
GAGGGTGTAG TGATTCCAGG TCCAGGCAAG AGAAAGAGAG AGCAGACAGA GGAGGAGTCC 
CAGGGAAGAC CGAGCCGCAG CCTGCGACGG ACCAAACCGA TGCAGGAGTC CACGGCCCCC 
AAAGTGCTGT TCACCGGTGT GGTGGATGCT CGCGGAGAGA GGACGGTGCT GGCCCTGGGG 
GGCAGTTTGG CCAGCTCGGT GGCCGAGGCT TCTCACCTGG TGACTGATCG GATCCGCCGG 
ACGGTCAAGT TTCTGTGTGC CCTGGGCCGG GGCATCCCCA TCCTCTCCCT GGCCTGGCTG 
CATGAGTCCC GCAAGGCAGG CTGCTTCTTG CCCCCGGACG AATATTTGGT GACTGATCCT 
GAGCAGGAGA AGAACTTCGG CTTCAGCCTT CGGGAGGCCC TGAGCCGAGC TCAGGAGCGA 
AGGCTGCTGG AGGGCTATGA GATTCACGTG ACCCCCGGAG TCCAGCCACC GCCACCTCAG 
ATGGGAGAAA TCATCAGCTG CTGTGGAGGC GCCATCCTGC CCAGCATGCC CCGGTCCTAC 
AAGCCTCAGA GGTTCGTGAT CACATGTTCC CAGGACTTTC CTCGATGTGC TGTTCCATAT 
CGGGTTGGGC TGCCTATCCT CTCACCCGAA TTCCTGCTGA CGGGAGTACT GAAGCAGGAA 
GTCAAGCCAG AGGCCTTTGC CTTCTCCACT GTGGAAATGT CATCCACCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011956374.1
CDS	143..5953
Translation	MEVSHFPCLHFPFKIMEDTQILNWEVEEEEEVAESPNESLGYSLEPLAQLHIFSSSYGPEKDFPLYLGKNMIGRMPDCSVALPFSSISKQHAVIEISAWDKAPVLRDCGSLNGTQILRPPKVLSPGVSHRLRDRELILFADLPCQYHRLDVPRPFVSRGPLSVEETPRVQGGTQPSRLLLAEDSEEEVDSLLDKCVVKGPRTSSSATVVPESDEEGPSPAPDGPGPPSAFNLNSDTDEEESQESGAGEASSAARRGTAAETEQPEPVTAEIQIEKDQCSVKERNGDTEIERDVRNGVVPTGVILERSQPSGEDSDTDVSDESGPRRRLVGVRPKRAWSCNFIDSDTDGEDEGIPATPAVVPMKERQIFHEAGTQSPQAPGVARWQESPADGDTDIEEGEAPPDRSQASVVIDSNTDDEEEVSAALTLACLRESQAGKWNRDPDAEETRAQPVALLERSQASAGGDSDTDVEEEGLPVERRGMVPKGYMDREYSKKSQHPPRDSDTEGKEDESSPGVHLERSQASAQVEDEVPLGPAVALPEKRQVQGIVWPHHTDAEAEGDPAQLPVLRLEEARPPLAGDCELDSENTSAVRKSQLPAEKDAGTTWAAAVPEQDRALAIGTQGGSSTAPGEQDLLPVSREDLADMVVDIGTPGEPQPQREGAQTTTGREREPYGNRATDSGDNLHDSEDLDLPATQCFVDRKNQSLEAAPSMEDEPTQAFLFPLPQEPGPSCCSSQATGSLDESWEALATQPFCPRESEASETQTVVTLLDTHASWPSPSRTAQQEQHPESPVHAEPLGMEGREMQTVEKEMGTPRETAERVIPEGGPPQEETKKLPSEGEREDVTGEEELIRGIQGSEKNQVFARDTQSQESDKKVKSASTGRGMEILKVEIETPKETQEKEREKQTLAGEIFESEAGKLVVERESEADGLEVKEPQELLDRSPQMGETEAGDQDQKGQASGLPSEPGAGAGDLQGLASDPVASGSQAGGGRGAPGSPRRQQRGDLNCEMPPAQKASRGDQESPDACLPPAAPEASAALPNSLIISQIQKHPAPQSLLFPSLAPLELPIPRTRQNESQEAPETPFSSELNSVHPEPKVRLQASSPVSSLPLEPHPTTPTGQPIALEPISGVSRSRTRSSFDVTASSVVPTAVALQPSTSTDKPVTPKPTLRAPRGRAQRSSVKTPEPNVTTDQPVAPELTAMATRGRAQRSSVKIPKPDNPTTPKPQPSTSTDQPVIPKPTSRAPRGRTPRSSAKTPEPAVPTASELQPAAPKDQPVAPELTSRATRGKTQRSIKTSKPDMPTTPEPQPSTSTDQPVTPKPTSRAPRGRTPRSSAKTPEPVVSTASELQPSALTDEPVTPELTSRATRGRAQRSSVKTPDPVTTTTPELQPSTSTDQLVTPKRTSRAPRGRTRRLSAKTPEPVVPTASELQPSAPADQPVGPWATQCRRHRSSVKTPEPVVPTVPEPQPSTSKDQSVTPEPTSQATESQTHRSSIKTSQPTEPTASDLKPSSPTDQLVTPKVIAQGGPSRTGKASTASAVLVPTTPEFQSPVPSEQPLPPDPIPEVNCSRRLRATRKHGSPTAHVHEPCTVPPEPNSHSSRNQRHGAVKAAKPLSTIPEPAFAQLPEAPPHTPQVPKEEAADGSGFTPEPQPRASQNHKRPSATAHSPPLQKRLQRGRVPQKAASLKEEENPVARPRKEEGVVIPGPGKRKREQTEEESQGRPSRSLRRTKPMQESTAPKVLFTGVVDARGERTVLALGGSLASSVAEASHLVTDRIRRTVKFLCALGRGIPILSLAWLHESRKAGCFLPPDEYLVTDPEQEKNFGFSLREALSRAQERRLLEGYEIHVTPGVQPPPPQMGEIISCCGGAILPSMPRSYKPQRFVITCSQDFPRCAVPYRVGLPILSPEFLLTGVLKQEVKPEAFAFSTVEMSST

Target ORF information:

RefSeq Version	XM_012100984.2
Organism	Ovis aries(sheep)
Definition	Ovis aries mediator of DNA damage checkpoint 1 (MDC1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012100984.2

ORF Insert Sequence:

ATGGAGGTGT CCCACTTTCC TTGTCTACAT TTTCCCTTTA AGATCATGGA AGACACCCAG 
ATTTTAAACT GGGAAGTTGA AGAAGAGGAG GAGGTTGCAG AGAGCCCCAA TGAATCTCTG 
GGGTATAGCT TGGAGCCCCT AGCACAGCTG CATATCTTCA GTAGTTCCTA TGGACCAGAA 
AAAGACTTTC CACTCTACCT TGGAAAGAAC ATGATAGGCC GAATGCCTGA TTGCTCTGTG 
GCCCTGCCCT TTTCATCAAT ATCCAAACAA CATGCAGTGA TTGAAATCTC TGCCTGGGAC 
AAGGCGCCTG TCCTTCGGGA TTGCGGGAGC CTCAATGGCA CTCAAATCCT GCGGCCTCCT 
AAGGTCCTGA GCCCTGGGGT GAGTCATCGT TTGCGGGACC GGGAGTTGAT TCTCTTTGCT 
GACTTGCCCT GCCAGTACCA TCGCTTGGAT GTCCCCCGGC CTTTTGTCTC CCGGGGCCCT 
CTAAGTGTAG AGGAGACACC CAGGGTACAG GGAGGAACTC AACCCTCCAG GCTTCTGTTG 
GCTGAGGACT CAGAGGAAGA AGTAGATTCC CTTTTGGACA AGTGTGTGGT GAAAGGACCA 
AGGACCTCCT CTTCGGCAAC AGTCGTTCCA GAGAGTGATG AAGAAGGGCC TTCCCCTGCC 
CCAGATGGGC CCGGGCCACC TTCTGCCTTC AACTTGAACA GCGACACGGA TGAGGAAGAA 
AGTCAGGAAT CAGGAGCAGG GGAGGCCTCT TCAGCTGCCA GAAGAGGTAC CGCTGCAGAG 
ACGGAACAGC CTGAACCTGT CACAGCCGAG ATCCAGATTG AGAAGGATCA GTGTTCCGTG 
AAGGAGAGGA ACGGGGACAC AGAAATCGAG AGGGATGTGA GGAATGGGGT GGTTCCGACT 
GGAGTGATTC TGGAGAGGAG CCAGCCTTCT GGGGAGGACA GTGACACAGA TGTGAGTGAT 
GAGAGTGGGC CTCGAAGAAG GCTGGTTGGA GTCCGTCCGA AAAGGGCCTG GTCTTGTAAC 
TTCATAGACA GTGATACCGA TGGAGAGGAC GAGGGGATCC CTGCTACCCC AGCAGTGGTT 
CCCATGAAGG AGAGGCAGAT CTTCCACGAA GCTGGTACAC AGAGCCCCCA GGCACCTGGT 
GTGGCACGTT GGCAGGAGAG CCCAGCTGAT GGTGATACAG ATATAGAGGA GGGGGAGGCC 
CCCCCAGACA GAAGCCAAGC CTCCGTGGTG ATCGACAGCA ATACAGACGA TGAGGAAGAA 
GTCTCAGCAG CACTGACACT GGCATGTCTA AGAGAGAGCC AGGCTGGTAA GTGGAACCGA 
GATCCAGATG CAGAAGAGAC CAGGGCCCAA CCCGTGGCCC TTCTGGAGCG AAGCCAGGCC 
TCTGCTGGGG GAGACAGTGA CACAGATGTG GAGGAAGAGG GGCTCCCAGT GGAAAGGAGG 
GGAATGGTTC CCAAGGGTTA CATGGACAGG GAATATTCAA AAAAGAGCCA GCACCCTCCC 
AGGGACAGTG ATACAGAGGG GAAGGAAGAT GAGAGCTCAC CGGGGGTCCA CCTGGAGAGA 
AGCCAGGCCT CTGCACAAGT GGAGGATGAG GTCCCACTGG GGCCAGCTGT TGCACTTCCG 
GAGAAGCGTC AGGTACAAGG CATAGTGTGG CCACATCACA CCGATGCGGA GGCAGAAGGG 
GACCCGGCAC AGCTCCCCGT GCTGCGTCTA GAGGAAGCCC GGCCTCCTCT AGCTGGGGAC 
TGTGAACTGG ATTCGGAGAA CACATCTGCT GTCAGAAAGA GCCAGCTTCC AGCGGAAAAA 
GATGCTGGGA CCACGTGGGC TGCAGCCGTT CCTGAACAGG ACAGAGCACT TGCCATCGGG 
ACCCAGGGTG GGTCATCCAC GGCACCAGGG GAGCAAGACC TTCTCCCGGT CTCAAGGGAA 
GACCTAGCAG ATATGGTGGT GGACATAGGC ACTCCAGGGG AGCCCCAACC GCAGAGAGAG 
GGGGCCCAGA CCACCACAGG AAGGGAGAGA GAACCATATG GGAATAGGGC CACAGACTCT 
GGAGACAACC TCCACGATTC TGAAGATCTG GACCTACCAG CTACCCAGTG CTTTGTAGAC 
AGAAAGAATC AGAGCCTGGA AGCAGCCCCC AGCATGGAGG ATGAGCCCAC CCAGGCCTTC 
CTGTTTCCTC TGCCCCAAGA GCCTGGCCCT TCCTGTTGCA GCTCCCAGGC CACAGGTTCC 
TTGGATGAGT CATGGGAGGC CTTGGCGACA CAGCCATTCT GTCCGAGAGA ATCTGAGGCC 
TCCGAGACCC AAACCGTTGT CACCCTCCTT GACACCCATG CATCTTGGCC CTCTCCATCT 
AGGACAGCAC AGCAAGAGCA ACATCCAGAG AGCCCAGTCC ATGCAGAGCC ACTGGGGATG 
GAAGGCAGAG AGATGCAGAC TGTGGAGAAA GAAATGGGTA CCCCAAGAGA AACAGCAGAG 
AGGGTGATCC CTGAGGGAGG GCCACCGCAG GAGGAAACCA AGAAACTGCC CTCAGAAGGA 
GAGAGGGAAG ATGTGACGGG AGAGGAAGAA TTAATCCGGG GGATACAGGG CAGTGAAAAA 
AATCAGGTGT TTGCTAGAGA TACTCAGAGC CAAGAATCTG ACAAAAAAGT GAAAAGTGCA 
AGTACTGGAA GGGGAATGGA GATTTTAAAG GTAGAAATTG AGACACCCAA GGAAACACAA 
GAGAAAGAGA GAGAAAAGCA GACTCTCGCA GGGGAAATAT TTGAGAGTGA AGCAGGGAAA 
CTGGTAGTAG AGAGAGAGAG TGAGGCAGAT GGGTTAGAAG TCAAGGAACC CCAAGAGCTA 
CTGGACAGAA GCCCACAGAT GGGGGAGACA GAGGCGGGGG ACCAGGACCA GAAAGGCCAA 
GCCTCTGGTC TGCCATCAGA GCCTGGAGCA GGGGCAGGAG ACCTTCAGGG ACTTGCTTCA 
GACCCAGTAG CTTCTGGGAG CCAGGCAGGT GGAGGAAGGG GAGCCCCAGG GAGCCCCAGG 
AGGCAGCAGA GAGGTGACTT GAATTGCGAG ATGCCACCTG CTCAGAAGGC TTCCAGGGGT 
GATCAGGAAT CCCCAGATGC TTGTCTGCCT CCTGCAGCGC CTGAAGCCTC AGCCGCACTC 
CCAAATTCCC TCATCATCTC TCAAATCCAA AAACATCCCG CACCTCAGTC CCTCCTTTTT 
CCCTCTCTAG CTCCTTTAGA ACTGCCTATT CCCAGGACCA GACAAAATGA GAGTCAGGAA 
GCTCCAGAGA CTCCCTTCTC CTCAGAGCTG AACTCTGTCC ACCCAGAACC CAAAGTCAGG 
CTCCAGGCGT CCTCTCCAGT TTCTTCTCTA CCCCTTGAGC CCCACCCTAC CACCCCCACA 
GGCCAGCCTA TTGCCCTTGA ACCCATATCT GGGGTCTCTC GGAGCAGGAC ACGTAGTTCC 
TTTGATGTAA CTGCCTCATC AGTTGTCCCC ACAGCCGTTG CACTGCAGCC ATCCACCTCC 
ACAGACAAGC CTGTCACCCC TAAGCCCACA CTTCGGGCCC CTCGGGGCAG GGCACAGAGG 
TCTTCTGTCA AAACCCCTGA ACCCAATGTC ACCACAGACC AGCCTGTTGC CCCTGAGCTC 
ACAGCTATGG CAACTCGGGG CAGGGCACAG AGGTCTTCTG TCAAGATTCC CAAACCAGAT 
AACCCCACAA CACCCAAGCC CCAGCCTTCC ACTTCCACAG ACCAGCCTGT CATCCCCAAA 
CCCACATCTC GGGCCCCTCG GGGCAGGACA CCTAGGTCTT CTGCCAAGAC TCCTGAACCA 
GCTGTCCCCA CAGCCTCTGA GCTCCAGCCT GCTGCCCCTA AAGACCAGCC TGTTGCTCCT 
GAGCTCACAT CTAGAGCCAC TCGGGGCAAG ACACAGAGGT CTATCAAGAC TTCCAAACCA 
GATATGCCCA CAACTCCCGA GCCCCAGCCT TCCACCTCCA CAGACCAGCC TGTCACCCCC 
AAACCCACGT CTCGGGCCCC TCGGGGCAGG ACACCTAGGT CTTCCGCCAA GACTCCTGAG 
CCAGTTGTCT CCACAGCCTC TGAGCTCCAG CCTTCTGCCC TCACAGATGA GCCTGTCACT 
CCTGAGCTCA CATCTAGGGC TACTCGGGGT CGGGCACAGA GGTCCTCTGT CAAAACCCCT 
GATCCAGTTA CCACCACAAC ACCTGAGCTC CAGCCTTCCA CCTCCACAGA CCAGCTTGTC 
ACCCCCAAAC GCACATCTCG GGCCCCTCGA GGCAGGACAC GTAGGTTGTC TGCCAAGACT 
CCTGAACCAG TTGTTCCCAC AGCCTCTGAG CTCCAGCCTT CTGCCCCTGC AGACCAGCCT 
GTTGGTCCTT GGGCCACTCA GTGTAGAAGA CATAGGTCTT CTGTCAAGAC CCCAGAACCA 
GTTGTCCCCA CAGTCCCTGA ACCTCAGCCT TCCACTTCTA AAGACCAGTC TGTCACTCCT 
GAGCCCACAT CTCAGGCCAC TGAGAGCCAA ACACATAGGT CCTCTATCAA GACATCCCAG 
CCAACGGAAC CCACAGCCTC TGACCTCAAA CCTTCCTCCC CCACAGACCA GCTTGTCACT 
CCCAAGGTCA TAGCTCAGGG TGGTCCAAGC AGGACAGGAA AGGCTTCCAC AGCAAGTGCT 
GTGCTGGTTC CTACTACCCC TGAATTCCAG TCTCCAGTCC CGTCAGAACA GCCTCTTCCC 
CCTGACCCCA TCCCTGAAGT CAACTGCAGC AGGAGGCTGA GGGCCACTAG GAAACATGGG 
TCTCCCACAG CTCATGTTCA TGAGCCCTGC ACCGTACCCC CTGAACCTAA CTCCCACTCT 
TCAAGGAACC AAAGACATGG GGCAGTGAAA GCAGCCAAGC CCCTTAGCAC CATTCCTGAG 
CCTGCCTTTG CCCAGCTCCC CGAGGCACCG CCTCACACTC CCCAGGTGCC AAAGGAGGAG 
GCAGCAGATG GATCAGGCTT CACCCCAGAG CCCCAGCCTA GGGCCTCTCA AAACCACAAG 
AGGCCTTCAG CTACTGCACA TTCACCTCCA CTTCAAAAAC GGCTCCAGAG AGGGAGAGTC 
CCTCAGAAGG CAGCATCCCT TAAGGAAGAA GAAAATCCAG TAGCGAGGCC GAGGAAGGAA 
GAGGGTGTAG TGATTCCAGG TCCAGGCAAG AGAAAGAGAG AGCAGACAGA GGAGGAGTCC 
CAGGGAAGAC CGAGCCGCAG CCTGCGACGG ACCAAACCGA TGCAGGAGTC CACGGCCCCC 
AAAGTGCTGT TCACCGGTGT GGTGGATGCT CGCGGAGAGA GGACGGTGCT GGCCCTGGGG 
GGCAGTTTGG CCAGCTCGGT GGCCGAGGCT TCTCACCTGG TGACTGATCG GATCCGCCGG 
ACGGTCAAGT TTCTGTGTGC CCTGGGCCGG GGCATCCCCA TCCTCTCCCT GGCCTGGCTG 
CATGAGTCCC GCAAGGCAGG CTGCTTCTTG CCCCCGGACG AATATTTGGT GACTGATCCT 
GAGCAGGAGA AGAACTTCGG CTTCAGCCTT CGGGAGGCCC TGAGCCGAGC TCAGGAGCGA 
AGGCTGCTGG AGGGCTATGA GATTCACGTG ACCCCCGGAG TCCAGCCACC GCCACCTCAG 
ATGGGAGAAA TCATCAGCTG CTGTGGAGGC GCCATCCTGC CCAGCATGCC CCGGTCCTAC 
AAGCCTCAGA GGTTCGTGAT CACATGTTCC CAGGACTTTC CTCGATGTGC TGTTCCATAT 
CGGGTTGGGC TGCCTATCCT CTCACCCGAA TTCCTGCTGA CGGGAGTACT GAAGCAGGAA 
GTCAAGCCAG AGGCCTTTGC CTTCTCCACT GTGGAAATGT CATCCACCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MDC1 ( XM_012100985.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_011956375.1 Ovis aries mediator of DNA damage checkpoint 1 (MDC1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OSm28026
Clone ID Related Accession (Same CDS sequence)	XM_012100985.2
Accession Version	XM_012100985.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5808bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries(sheep)
Product	mediator of DNA damage checkpoint protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014639029.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012100985.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGTGT CCCACTTTCC TTGTCTACAT TTTCCCTTTA AGATCATGGA AGACACCCAG 
ATTTTAAACT GGGAAGTTGA AGAAGAGGAG GAGGTTGCAG AGAGCCCCAA TGAATCTCTG 
GGGTATAGCT TGGAGCCCCT AGCACAGCTG CATATCTTCA GTAGTTCCTA TGGACCAGAA 
AAAGACTTTC CACTCTACCT TGGAAAGAAC ATGATAGGCC GAATGCCTGA TTGCTCTGTG 
GCCCTGCCCT TTTCATCAAT ATCCAAACAA CATGCAGTGA TTGAAATCTC TGCCTGGGAC 
AAGGCGCCTG TCCTTCGGGA TTGCGGGAGC CTCAATGGCA CTCAAATCCT GCGGCCTCCT 
AAGGTCCTGA GCCCTGGGGT GAGTCATCGT TTGCGGGACC GGGAGTTGAT TCTCTTTGCT 
GACTTGCCCT GCCAGTACCA TCGCTTGGAT GTCCCCCGGC CTTTTGTCTC CCGGGGCCCT 
CTAAGTGTAG AGGAGACACC CAGGGTACAG GGAGGAACTC AACCCTCCAG GCTTCTGTTG 
GCTGAGGACT CAGAGGAAGA AGTAGATTCC CTTTTGGACA AGTGTGTGGT GAAAGGACCA 
AGGACCTCCT CTTCGGCAAC AGTCGTTCCA GAGAGTGATG AAGAAGGGCC TTCCCCTGCC 
CCAGATGGGC CCGGGCCACC TTCTGCCTTC AACTTGAACA GCGACACGGA TGAGGAAGAA 
AGTCAGGAAT CAGGAGCAGG GGAGGCCTCT TCAGCTGCCA GAAGAGGTAC CGCTGCAGAG 
ACGGAACAGC CTGAACCTGT CACAGCCGAG ATCCAGATTG AGAAGGATCA GTGTTCCGTG 
AAGGAGAGGA ACGGGGACAC AGAAATCGAG AGGGATGTGA GGAATGGGGT GGTTCCGACT 
GGAGTGATTC TGGAGAGGAG CCAGCCTTCT GGGGAGGACA GTGACACAGA TGTGAGTGAT 
GAGAGTGGGC CTCGAAGAAG GCTGGTTGGA GTCCGTCCGA AAAGGGCCTG GTCTTGTAAC 
TTCATAGACA GTGATACCGA TGGAGAGGAC GAGGGGATCC CTGCTACCCC AGCAGTGGTT 
CCCATGAAGG AGAGGCAGAT CTTCCACGAA GCTGGTACAC AGAGCCCCCA GGCACCTGGT 
GTGGCACGTT GGCAGGAGAG CCCAGCTGAT GGTGATACAG ATATAGAGGA GGGGGAGGCC 
CCCCCAGACA GAAGCCAAGC CTCCGTGGTG ATCGACAGCA ATACAGACGA TGAGGAAGAA 
GTCTCAGCAG CACTGACACT GGCATGTCTA AGAGAGAGCC AGGCTGGTAA GTGGAACCGA 
GATCCAGATG CAGAAGAGAC CAGGGCCCAA CCCGTGGCCC TTCTGGAGCG AAGCCAGGCC 
TCTGCTGGGG GAGACAGTGA CACAGATGTG GAGGAAGAGG GGCTCCCAGT GGAAAGGAGG 
GGAATGGTTC CCAAGGGTTA CATGGACAGG GAATATTCAA AAAAGAGCCA GCACCCTCCC 
AGGGACAGTG ATACAGAGGG GAAGGAAGAT GAGAGCTCAC CGGGGGTCCA CCTGGAGAGA 
AGCCAGGCCT CTGCACAAGT GGAGGATGAG GTCCCACTGG GGCCAGCTGT TGCACTTCCG 
GAGAAGCGTC AGGTACAAGG CATAGTGTGG CCACATCACA CCGATGCGGA GGCAGAAGGG 
GACCCGGCAC AGCTCCCCGT GCTGCGTCTA GAGGAAGCCC GGCCTCCTCT AGCTGGGGAC 
TGTGAACTGG ATTCGGAGAA CACATCTGCT GTCAGAAAGA GCCAGCTTCC AGCGGAAAAA 
GATGCTGGGA CCACGTGGGC TGCAGCCGTT CCTGAACAGG ACAGAGCACT TGCCATCGGG 
ACCCAGGGTG GGTCATCCAC GGCACCAGGG GAGCAAGACC TTCTCCCGGT CTCAAGGGAA 
GACCTAGCAG ATATGGTGGT GGACATAGGC ACTCCAGGGG AGCCCCAACC GCAGAGAGAG 
GGGGCCCAGA CCACCACAGG AAGGGAGAGA GAACCATATG GGAATAGGGC CACAGACTCT 
GGAGACAACC TCCACGATTC TGAAGATCTG GACCTACCAG CTACCCAGTG CTTTGTAGAC 
AGAAAGAATC AGAGCCTGGA AGCCCCCAGC ATGGAGGATG AGCCCACCCA GGCCTTCCTG 
TTTCCTCTGC CCCAAGAGCC TGGCCCTTCC TGTTGCAGCT CCCAGGCCAC AGGTTCCTTG 
GATGAGTCAT GGGAGGCCTT GGCGACACAG CCATTCTGTC CGAGAGAATC TGAGGCCTCC 
GAGACCCAAA CCGTTGTCAC CCTCCTTGAC ACCCATGCAT CTTGGCCCTC TCCATCTAGG 
ACAGCACAGC AAGAGCAACA TCCAGAGAGC CCAGTCCATG CAGAGCCACT GGGGATGGAA 
GGCAGAGAGA TGCAGACTGT GGAGAAAGAA ATGGGTACCC CAAGAGAAAC AGCAGAGAGG 
GTGATCCCTG AGGGAGGGCC ACCGCAGGAG GAAACCAAGA AACTGCCCTC AGAAGGAGAG 
AGGGAAGATG TGACGGGAGA GGAAGAATTA ATCCGGGGGA TACAGGGCAG TGAAAAAAAT 
CAGGTGTTTG CTAGAGATAC TCAGAGCCAA GAATCTGACA AAAAAGTGAA AAGTGCAAGT 
ACTGGAAGGG GAATGGAGAT TTTAAAGGTA GAAATTGAGA CACCCAAGGA AACACAAGAG 
AAAGAGAGAG AAAAGCAGAC TCTCGCAGGG GAAATATTTG AGAGTGAAGC AGGGAAACTG 
GTAGTAGAGA GAGAGAGTGA GGCAGATGGG TTAGAAGTCA AGGAACCCCA AGAGCTACTG 
GACAGAAGCC CACAGATGGG GGAGACAGAG GCGGGGGACC AGGACCAGAA AGGCCAAGCC 
TCTGGTCTGC CATCAGAGCC TGGAGCAGGG GCAGGAGACC TTCAGGGACT TGCTTCAGAC 
CCAGTAGCTT CTGGGAGCCA GGCAGGTGGA GGAAGGGGAG CCCCAGGGAG CCCCAGGAGG 
CAGCAGAGAG GTGACTTGAA TTGCGAGATG CCACCTGCTC AGAAGGCTTC CAGGGGTGAT 
CAGGAATCCC CAGATGCTTG TCTGCCTCCT GCAGCGCCTG AAGCCTCAGC CGCACTCCCA 
AATTCCCTCA TCATCTCTCA AATCCAAAAA CATCCCGCAC CTCAGTCCCT CCTTTTTCCC 
TCTCTAGCTC CTTTAGAACT GCCTATTCCC AGGACCAGAC AAAATGAGAG TCAGGAAGCT 
CCAGAGACTC CCTTCTCCTC AGAGCTGAAC TCTGTCCACC CAGAACCCAA AGTCAGGCTC 
CAGGCGTCCT CTCCAGTTTC TTCTCTACCC CTTGAGCCCC ACCCTACCAC CCCCACAGGC 
CAGCCTATTG CCCTTGAACC CATATCTGGG GTCTCTCGGA GCAGGACACG TAGTTCCTTT 
GATGTAACTG CCTCATCAGT TGTCCCCACA GCCGTTGCAC TGCAGCCATC CACCTCCACA 
GACAAGCCTG TCACCCCTAA GCCCACACTT CGGGCCCCTC GGGGCAGGGC ACAGAGGTCT 
TCTGTCAAAA CCCCTGAACC CAATGTCACC ACAGACCAGC CTGTTGCCCC TGAGCTCACA 
GCTATGGCAA CTCGGGGCAG GGCACAGAGG TCTTCTGTCA AGATTCCCAA ACCAGATAAC 
CCCACAACAC CCAAGCCCCA GCCTTCCACT TCCACAGACC AGCCTGTCAT CCCCAAACCC 
ACATCTCGGG CCCCTCGGGG CAGGACACCT AGGTCTTCTG CCAAGACTCC TGAACCAGCT 
GTCCCCACAG CCTCTGAGCT CCAGCCTGCT GCCCCTAAAG ACCAGCCTGT TGCTCCTGAG 
CTCACATCTA GAGCCACTCG GGGCAAGACA CAGAGGTCTA TCAAGACTTC CAAACCAGAT 
ATGCCCACAA CTCCCGAGCC CCAGCCTTCC ACCTCCACAG ACCAGCCTGT CACCCCCAAA 
CCCACGTCTC GGGCCCCTCG GGGCAGGACA CCTAGGTCTT CCGCCAAGAC TCCTGAGCCA 
GTTGTCTCCA CAGCCTCTGA GCTCCAGCCT TCTGCCCTCA CAGATGAGCC TGTCACTCCT 
GAGCTCACAT CTAGGGCTAC TCGGGGTCGG GCACAGAGGT CCTCTGTCAA AACCCCTGAT 
CCAGTTACCA CCACAACACC TGAGCTCCAG CCTTCCACCT CCACAGACCA GCTTGTCACC 
CCCAAACGCA CATCTCGGGC CCCTCGAGGC AGGACACGTA GGTTGTCTGC CAAGACTCCT 
GAACCAGTTG TTCCCACAGC CTCTGAGCTC CAGCCTTCTG CCCCTGCAGA CCAGCCTGTT 
GGTCCTTGGG CCACTCAGTG TAGAAGACAT AGGTCTTCTG TCAAGACCCC AGAACCAGTT 
GTCCCCACAG TCCCTGAACC TCAGCCTTCC ACTTCTAAAG ACCAGTCTGT CACTCCTGAG 
CCCACATCTC AGGCCACTGA GAGCCAAACA CATAGGTCCT CTATCAAGAC ATCCCAGCCA 
ACGGAACCCA CAGCCTCTGA CCTCAAACCT TCCTCCCCCA CAGACCAGCT TGTCACTCCC 
AAGGTCATAG CTCAGGGTGG TCCAAGCAGG ACAGGAAAGG CTTCCACAGC AAGTGCTGTG 
CTGGTTCCTA CTACCCCTGA ATTCCAGTCT CCAGTCCCGT CAGAACAGCC TCTTCCCCCT 
GACCCCATCC CTGAAGTCAA CTGCAGCAGG AGGCTGAGGG CCACTAGGAA ACATGGGTCT 
CCCACAGCTC ATGTTCATGA GCCCTGCACC GTACCCCCTG AACCTAACTC CCACTCTTCA 
AGGAACCAAA GACATGGGGC AGTGAAAGCA GCCAAGCCCC TTAGCACCAT TCCTGAGCCT 
GCCTTTGCCC AGCTCCCCGA GGCACCGCCT CACACTCCCC AGGTGCCAAA GGAGGAGGCA 
GCAGATGGAT CAGGCTTCAC CCCAGAGCCC CAGCCTAGGG CCTCTCAAAA CCACAAGAGG 
CCTTCAGCTA CTGCACATTC ACCTCCACTT CAAAAACGGC TCCAGAGAGG GAGAGTCCCT 
CAGAAGGCAG CATCCCTTAA GGAAGAAGAA AATCCAGTAG CGAGGCCGAG GAAGGAAGAG 
GGTGTAGTGA TTCCAGGTCC AGGCAAGAGA AAGAGAGAGC AGACAGAGGA GGAGTCCCAG 
GGAAGACCGA GCCGCAGCCT GCGACGGACC AAACCGATGC AGGAGTCCAC GGCCCCCAAA 
GTGCTGTTCA CCGGTGTGGT GGATGCTCGC GGAGAGAGGA CGGTGCTGGC CCTGGGGGGC 
AGTTTGGCCA GCTCGGTGGC CGAGGCTTCT CACCTGGTGA CTGATCGGAT CCGCCGGACG 
GTCAAGTTTC TGTGTGCCCT GGGCCGGGGC ATCCCCATCC TCTCCCTGGC CTGGCTGCAT 
GAGTCCCGCA AGGCAGGCTG CTTCTTGCCC CCGGACGAAT ATTTGGTGAC TGATCCTGAG 
CAGGAGAAGA ACTTCGGCTT CAGCCTTCGG GAGGCCCTGA GCCGAGCTCA GGAGCGAAGG 
CTGCTGGAGG GCTATGAGAT TCACGTGACC CCCGGAGTCC AGCCACCGCC ACCTCAGATG 
GGAGAAATCA TCAGCTGCTG TGGAGGCGCC ATCCTGCCCA GCATGCCCCG GTCCTACAAG 
CCTCAGAGGT TCGTGATCAC ATGTTCCCAG GACTTTCCTC GATGTGCTGT TCCATATCGG 
GTTGGGCTGC CTATCCTCTC ACCCGAATTC CTGCTGACGG GAGTACTGAA GCAGGAAGTC 
AAGCCAGAGG CCTTTGCCTT CTCCACTGTG GAAATGTCAT CCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011956375.1
CDS	144..5951
Translation	MEVSHFPCLHFPFKIMEDTQILNWEVEEEEEVAESPNESLGYSLEPLAQLHIFSSSYGPEKDFPLYLGKNMIGRMPDCSVALPFSSISKQHAVIEISAWDKAPVLRDCGSLNGTQILRPPKVLSPGVSHRLRDRELILFADLPCQYHRLDVPRPFVSRGPLSVEETPRVQGGTQPSRLLLAEDSEEEVDSLLDKCVVKGPRTSSSATVVPESDEEGPSPAPDGPGPPSAFNLNSDTDEEESQESGAGEASSAARRGTAAETEQPEPVTAEIQIEKDQCSVKERNGDTEIERDVRNGVVPTGVILERSQPSGEDSDTDVSDESGPRRRLVGVRPKRAWSCNFIDSDTDGEDEGIPATPAVVPMKERQIFHEAGTQSPQAPGVARWQESPADGDTDIEEGEAPPDRSQASVVIDSNTDDEEEVSAALTLACLRESQAGKWNRDPDAEETRAQPVALLERSQASAGGDSDTDVEEEGLPVERRGMVPKGYMDREYSKKSQHPPRDSDTEGKEDESSPGVHLERSQASAQVEDEVPLGPAVALPEKRQVQGIVWPHHTDAEAEGDPAQLPVLRLEEARPPLAGDCELDSENTSAVRKSQLPAEKDAGTTWAAAVPEQDRALAIGTQGGSSTAPGEQDLLPVSREDLADMVVDIGTPGEPQPQREGAQTTTGREREPYGNRATDSGDNLHDSEDLDLPATQCFVDRKNQSLEAPSMEDEPTQAFLFPLPQEPGPSCCSSQATGSLDESWEALATQPFCPRESEASETQTVVTLLDTHASWPSPSRTAQQEQHPESPVHAEPLGMEGREMQTVEKEMGTPRETAERVIPEGGPPQEETKKLPSEGEREDVTGEEELIRGIQGSEKNQVFARDTQSQESDKKVKSASTGRGMEILKVEIETPKETQEKEREKQTLAGEIFESEAGKLVVERESEADGLEVKEPQELLDRSPQMGETEAGDQDQKGQASGLPSEPGAGAGDLQGLASDPVASGSQAGGGRGAPGSPRRQQRGDLNCEMPPAQKASRGDQESPDACLPPAAPEASAALPNSLIISQIQKHPAPQSLLFPSLAPLELPIPRTRQNESQEAPETPFSSELNSVHPEPKVRLQASSPVSSLPLEPHPTTPTGQPIALEPISGVSRSRTRSSFDVTASSVVPTAVALQPSTSTDKPVTPKPTLRAPRGRAQRSSVKTPEPNVTTDQPVAPELTAMATRGRAQRSSVKIPKPDNPTTPKPQPSTSTDQPVIPKPTSRAPRGRTPRSSAKTPEPAVPTASELQPAAPKDQPVAPELTSRATRGKTQRSIKTSKPDMPTTPEPQPSTSTDQPVTPKPTSRAPRGRTPRSSAKTPEPVVSTASELQPSALTDEPVTPELTSRATRGRAQRSSVKTPDPVTTTTPELQPSTSTDQLVTPKRTSRAPRGRTRRLSAKTPEPVVPTASELQPSAPADQPVGPWATQCRRHRSSVKTPEPVVPTVPEPQPSTSKDQSVTPEPTSQATESQTHRSSIKTSQPTEPTASDLKPSSPTDQLVTPKVIAQGGPSRTGKASTASAVLVPTTPEFQSPVPSEQPLPPDPIPEVNCSRRLRATRKHGSPTAHVHEPCTVPPEPNSHSSRNQRHGAVKAAKPLSTIPEPAFAQLPEAPPHTPQVPKEEAADGSGFTPEPQPRASQNHKRPSATAHSPPLQKRLQRGRVPQKAASLKEEENPVARPRKEEGVVIPGPGKRKREQTEEESQGRPSRSLRRTKPMQESTAPKVLFTGVVDARGERTVLALGGSLASSVAEASHLVTDRIRRTVKFLCALGRGIPILSLAWLHESRKAGCFLPPDEYLVTDPEQEKNFGFSLREALSRAQERRLLEGYEIHVTPGVQPPPPQMGEIISCCGGAILPSMPRSYKPQRFVITCSQDFPRCAVPYRVGLPILSPEFLLTGVLKQEVKPEAFAFSTVEMSST

Target ORF information:

RefSeq Version	XM_012100985.2
Organism	Ovis aries(sheep)
Definition	Ovis aries mediator of DNA damage checkpoint 1 (MDC1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012100985.2

ORF Insert Sequence:

ATGGAGGTGT CCCACTTTCC TTGTCTACAT TTTCCCTTTA AGATCATGGA AGACACCCAG 
ATTTTAAACT GGGAAGTTGA AGAAGAGGAG GAGGTTGCAG AGAGCCCCAA TGAATCTCTG 
GGGTATAGCT TGGAGCCCCT AGCACAGCTG CATATCTTCA GTAGTTCCTA TGGACCAGAA 
AAAGACTTTC CACTCTACCT TGGAAAGAAC ATGATAGGCC GAATGCCTGA TTGCTCTGTG 
GCCCTGCCCT TTTCATCAAT ATCCAAACAA CATGCAGTGA TTGAAATCTC TGCCTGGGAC 
AAGGCGCCTG TCCTTCGGGA TTGCGGGAGC CTCAATGGCA CTCAAATCCT GCGGCCTCCT 
AAGGTCCTGA GCCCTGGGGT GAGTCATCGT TTGCGGGACC GGGAGTTGAT TCTCTTTGCT 
GACTTGCCCT GCCAGTACCA TCGCTTGGAT GTCCCCCGGC CTTTTGTCTC CCGGGGCCCT 
CTAAGTGTAG AGGAGACACC CAGGGTACAG GGAGGAACTC AACCCTCCAG GCTTCTGTTG 
GCTGAGGACT CAGAGGAAGA AGTAGATTCC CTTTTGGACA AGTGTGTGGT GAAAGGACCA 
AGGACCTCCT CTTCGGCAAC AGTCGTTCCA GAGAGTGATG AAGAAGGGCC TTCCCCTGCC 
CCAGATGGGC CCGGGCCACC TTCTGCCTTC AACTTGAACA GCGACACGGA TGAGGAAGAA 
AGTCAGGAAT CAGGAGCAGG GGAGGCCTCT TCAGCTGCCA GAAGAGGTAC CGCTGCAGAG 
ACGGAACAGC CTGAACCTGT CACAGCCGAG ATCCAGATTG AGAAGGATCA GTGTTCCGTG 
AAGGAGAGGA ACGGGGACAC AGAAATCGAG AGGGATGTGA GGAATGGGGT GGTTCCGACT 
GGAGTGATTC TGGAGAGGAG CCAGCCTTCT GGGGAGGACA GTGACACAGA TGTGAGTGAT 
GAGAGTGGGC CTCGAAGAAG GCTGGTTGGA GTCCGTCCGA AAAGGGCCTG GTCTTGTAAC 
TTCATAGACA GTGATACCGA TGGAGAGGAC GAGGGGATCC CTGCTACCCC AGCAGTGGTT 
CCCATGAAGG AGAGGCAGAT CTTCCACGAA GCTGGTACAC AGAGCCCCCA GGCACCTGGT 
GTGGCACGTT GGCAGGAGAG CCCAGCTGAT GGTGATACAG ATATAGAGGA GGGGGAGGCC 
CCCCCAGACA GAAGCCAAGC CTCCGTGGTG ATCGACAGCA ATACAGACGA TGAGGAAGAA 
GTCTCAGCAG CACTGACACT GGCATGTCTA AGAGAGAGCC AGGCTGGTAA GTGGAACCGA 
GATCCAGATG CAGAAGAGAC CAGGGCCCAA CCCGTGGCCC TTCTGGAGCG AAGCCAGGCC 
TCTGCTGGGG GAGACAGTGA CACAGATGTG GAGGAAGAGG GGCTCCCAGT GGAAAGGAGG 
GGAATGGTTC CCAAGGGTTA CATGGACAGG GAATATTCAA AAAAGAGCCA GCACCCTCCC 
AGGGACAGTG ATACAGAGGG GAAGGAAGAT GAGAGCTCAC CGGGGGTCCA CCTGGAGAGA 
AGCCAGGCCT CTGCACAAGT GGAGGATGAG GTCCCACTGG GGCCAGCTGT TGCACTTCCG 
GAGAAGCGTC AGGTACAAGG CATAGTGTGG CCACATCACA CCGATGCGGA GGCAGAAGGG 
GACCCGGCAC AGCTCCCCGT GCTGCGTCTA GAGGAAGCCC GGCCTCCTCT AGCTGGGGAC 
TGTGAACTGG ATTCGGAGAA CACATCTGCT GTCAGAAAGA GCCAGCTTCC AGCGGAAAAA 
GATGCTGGGA CCACGTGGGC TGCAGCCGTT CCTGAACAGG ACAGAGCACT TGCCATCGGG 
ACCCAGGGTG GGTCATCCAC GGCACCAGGG GAGCAAGACC TTCTCCCGGT CTCAAGGGAA 
GACCTAGCAG ATATGGTGGT GGACATAGGC ACTCCAGGGG AGCCCCAACC GCAGAGAGAG 
GGGGCCCAGA CCACCACAGG AAGGGAGAGA GAACCATATG GGAATAGGGC CACAGACTCT 
GGAGACAACC TCCACGATTC TGAAGATCTG GACCTACCAG CTACCCAGTG CTTTGTAGAC 
AGAAAGAATC AGAGCCTGGA AGCCCCCAGC ATGGAGGATG AGCCCACCCA GGCCTTCCTG 
TTTCCTCTGC CCCAAGAGCC TGGCCCTTCC TGTTGCAGCT CCCAGGCCAC AGGTTCCTTG 
GATGAGTCAT GGGAGGCCTT GGCGACACAG CCATTCTGTC CGAGAGAATC TGAGGCCTCC 
GAGACCCAAA CCGTTGTCAC CCTCCTTGAC ACCCATGCAT CTTGGCCCTC TCCATCTAGG 
ACAGCACAGC AAGAGCAACA TCCAGAGAGC CCAGTCCATG CAGAGCCACT GGGGATGGAA 
GGCAGAGAGA TGCAGACTGT GGAGAAAGAA ATGGGTACCC CAAGAGAAAC AGCAGAGAGG 
GTGATCCCTG AGGGAGGGCC ACCGCAGGAG GAAACCAAGA AACTGCCCTC AGAAGGAGAG 
AGGGAAGATG TGACGGGAGA GGAAGAATTA ATCCGGGGGA TACAGGGCAG TGAAAAAAAT 
CAGGTGTTTG CTAGAGATAC TCAGAGCCAA GAATCTGACA AAAAAGTGAA AAGTGCAAGT 
ACTGGAAGGG GAATGGAGAT TTTAAAGGTA GAAATTGAGA CACCCAAGGA AACACAAGAG 
AAAGAGAGAG AAAAGCAGAC TCTCGCAGGG GAAATATTTG AGAGTGAAGC AGGGAAACTG 
GTAGTAGAGA GAGAGAGTGA GGCAGATGGG TTAGAAGTCA AGGAACCCCA AGAGCTACTG 
GACAGAAGCC CACAGATGGG GGAGACAGAG GCGGGGGACC AGGACCAGAA AGGCCAAGCC 
TCTGGTCTGC CATCAGAGCC TGGAGCAGGG GCAGGAGACC TTCAGGGACT TGCTTCAGAC 
CCAGTAGCTT CTGGGAGCCA GGCAGGTGGA GGAAGGGGAG CCCCAGGGAG CCCCAGGAGG 
CAGCAGAGAG GTGACTTGAA TTGCGAGATG CCACCTGCTC AGAAGGCTTC CAGGGGTGAT 
CAGGAATCCC CAGATGCTTG TCTGCCTCCT GCAGCGCCTG AAGCCTCAGC CGCACTCCCA 
AATTCCCTCA TCATCTCTCA AATCCAAAAA CATCCCGCAC CTCAGTCCCT CCTTTTTCCC 
TCTCTAGCTC CTTTAGAACT GCCTATTCCC AGGACCAGAC AAAATGAGAG TCAGGAAGCT 
CCAGAGACTC CCTTCTCCTC AGAGCTGAAC TCTGTCCACC CAGAACCCAA AGTCAGGCTC 
CAGGCGTCCT CTCCAGTTTC TTCTCTACCC CTTGAGCCCC ACCCTACCAC CCCCACAGGC 
CAGCCTATTG CCCTTGAACC CATATCTGGG GTCTCTCGGA GCAGGACACG TAGTTCCTTT 
GATGTAACTG CCTCATCAGT TGTCCCCACA GCCGTTGCAC TGCAGCCATC CACCTCCACA 
GACAAGCCTG TCACCCCTAA GCCCACACTT CGGGCCCCTC GGGGCAGGGC ACAGAGGTCT 
TCTGTCAAAA CCCCTGAACC CAATGTCACC ACAGACCAGC CTGTTGCCCC TGAGCTCACA 
GCTATGGCAA CTCGGGGCAG GGCACAGAGG TCTTCTGTCA AGATTCCCAA ACCAGATAAC 
CCCACAACAC CCAAGCCCCA GCCTTCCACT TCCACAGACC AGCCTGTCAT CCCCAAACCC 
ACATCTCGGG CCCCTCGGGG CAGGACACCT AGGTCTTCTG CCAAGACTCC TGAACCAGCT 
GTCCCCACAG CCTCTGAGCT CCAGCCTGCT GCCCCTAAAG ACCAGCCTGT TGCTCCTGAG 
CTCACATCTA GAGCCACTCG GGGCAAGACA CAGAGGTCTA TCAAGACTTC CAAACCAGAT 
ATGCCCACAA CTCCCGAGCC CCAGCCTTCC ACCTCCACAG ACCAGCCTGT CACCCCCAAA 
CCCACGTCTC GGGCCCCTCG GGGCAGGACA CCTAGGTCTT CCGCCAAGAC TCCTGAGCCA 
GTTGTCTCCA CAGCCTCTGA GCTCCAGCCT TCTGCCCTCA CAGATGAGCC TGTCACTCCT 
GAGCTCACAT CTAGGGCTAC TCGGGGTCGG GCACAGAGGT CCTCTGTCAA AACCCCTGAT 
CCAGTTACCA CCACAACACC TGAGCTCCAG CCTTCCACCT CCACAGACCA GCTTGTCACC 
CCCAAACGCA CATCTCGGGC CCCTCGAGGC AGGACACGTA GGTTGTCTGC CAAGACTCCT 
GAACCAGTTG TTCCCACAGC CTCTGAGCTC CAGCCTTCTG CCCCTGCAGA CCAGCCTGTT 
GGTCCTTGGG CCACTCAGTG TAGAAGACAT AGGTCTTCTG TCAAGACCCC AGAACCAGTT 
GTCCCCACAG TCCCTGAACC TCAGCCTTCC ACTTCTAAAG ACCAGTCTGT CACTCCTGAG 
CCCACATCTC AGGCCACTGA GAGCCAAACA CATAGGTCCT CTATCAAGAC ATCCCAGCCA 
ACGGAACCCA CAGCCTCTGA CCTCAAACCT TCCTCCCCCA CAGACCAGCT TGTCACTCCC 
AAGGTCATAG CTCAGGGTGG TCCAAGCAGG ACAGGAAAGG CTTCCACAGC AAGTGCTGTG 
CTGGTTCCTA CTACCCCTGA ATTCCAGTCT CCAGTCCCGT CAGAACAGCC TCTTCCCCCT 
GACCCCATCC CTGAAGTCAA CTGCAGCAGG AGGCTGAGGG CCACTAGGAA ACATGGGTCT 
CCCACAGCTC ATGTTCATGA GCCCTGCACC GTACCCCCTG AACCTAACTC CCACTCTTCA 
AGGAACCAAA GACATGGGGC AGTGAAAGCA GCCAAGCCCC TTAGCACCAT TCCTGAGCCT 
GCCTTTGCCC AGCTCCCCGA GGCACCGCCT CACACTCCCC AGGTGCCAAA GGAGGAGGCA 
GCAGATGGAT CAGGCTTCAC CCCAGAGCCC CAGCCTAGGG CCTCTCAAAA CCACAAGAGG 
CCTTCAGCTA CTGCACATTC ACCTCCACTT CAAAAACGGC TCCAGAGAGG GAGAGTCCCT 
CAGAAGGCAG CATCCCTTAA GGAAGAAGAA AATCCAGTAG CGAGGCCGAG GAAGGAAGAG 
GGTGTAGTGA TTCCAGGTCC AGGCAAGAGA AAGAGAGAGC AGACAGAGGA GGAGTCCCAG 
GGAAGACCGA GCCGCAGCCT GCGACGGACC AAACCGATGC AGGAGTCCAC GGCCCCCAAA 
GTGCTGTTCA CCGGTGTGGT GGATGCTCGC GGAGAGAGGA CGGTGCTGGC CCTGGGGGGC 
AGTTTGGCCA GCTCGGTGGC CGAGGCTTCT CACCTGGTGA CTGATCGGAT CCGCCGGACG 
GTCAAGTTTC TGTGTGCCCT GGGCCGGGGC ATCCCCATCC TCTCCCTGGC CTGGCTGCAT 
GAGTCCCGCA AGGCAGGCTG CTTCTTGCCC CCGGACGAAT ATTTGGTGAC TGATCCTGAG 
CAGGAGAAGA ACTTCGGCTT CAGCCTTCGG GAGGCCCTGA GCCGAGCTCA GGAGCGAAGG 
CTGCTGGAGG GCTATGAGAT TCACGTGACC CCCGGAGTCC AGCCACCGCC ACCTCAGATG 
GGAGAAATCA TCAGCTGCTG TGGAGGCGCC ATCCTGCCCA GCATGCCCCG GTCCTACAAG 
CCTCAGAGGT TCGTGATCAC ATGTTCCCAG GACTTTCCTC GATGTGCTGT TCCATATCGG 
GTTGGGCTGC CTATCCTCTC ACCCGAATTC CTGCTGACGG GAGTACTGAA GCAGGAAGTC 
AAGCCAGAGG CCTTTGCCTT CTCCACTGTG GAAATGTCAT CCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MDC1 ( XM_012100986.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_011956376.1 Ovis aries mediator of DNA damage checkpoint 1 (MDC1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OSm28027
Clone ID Related Accession (Same CDS sequence)	XM_012100986.2
Accession Version	XM_012100986.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5766bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries(sheep)
Product	mediator of DNA damage checkpoint protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014639029.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012100986.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGACA CCCAGATTTT AAACTGGGAA GTTGAAGAAG AGGAGGAGGT TGCAGAGAGC 
CCCAATGAAT CTCTGGGGTA TAGCTTGGAG CCCCTAGCAC AGCTGCATAT CTTCAGTAGT 
TCCTATGGAC CAGAAAAAGA CTTTCCACTC TACCTTGGAA AGAACATGAT AGGCCGAATG 
CCTGATTGCT CTGTGGCCCT GCCCTTTTCA TCAATATCCA AACAACATGC AGTGATTGAA 
ATCTCTGCCT GGGACAAGGC GCCTGTCCTT CGGGATTGCG GGAGCCTCAA TGGCACTCAA 
ATCCTGCGGC CTCCTAAGGT CCTGAGCCCT GGGGTGAGTC ATCGTTTGCG GGACCGGGAG 
TTGATTCTCT TTGCTGACTT GCCCTGCCAG TACCATCGCT TGGATGTCCC CCGGCCTTTT 
GTCTCCCGGG GCCCTCTAAG TGTAGAGGAG ACACCCAGGG TACAGGGAGG AACTCAACCC 
TCCAGGCTTC TGTTGGCTGA GGACTCAGAG GAAGAAGTAG ATTCCCTTTT GGACAAGTGT 
GTGGTGAAAG GACCAAGGAC CTCCTCTTCG GCAACAGTCG TTCCAGAGAG TGATGAAGAA 
GGGCCTTCCC CTGCCCCAGA TGGGCCCGGG CCACCTTCTG CCTTCAACTT GAACAGCGAC 
ACGGATGAGG AAGAAAGTCA GGAATCAGGA GCAGGGGAGG CCTCTTCAGC TGCCAGAAGA 
GGTACCGCTG CAGAGACGGA ACAGCCTGAA CCTGTCACAG CCGAGATCCA GATTGAGAAG 
GATCAGTGTT CCGTGAAGGA GAGGAACGGG GACACAGAAA TCGAGAGGGA TGTGAGGAAT 
GGGGTGGTTC CGACTGGAGT GATTCTGGAG AGGAGCCAGC CTTCTGGGGA GGACAGTGAC 
ACAGATGTGA GTGATGAGAG TGGGCCTCGA AGAAGGCTGG TTGGAGTCCG TCCGAAAAGG 
GCCTGGTCTT GTAACTTCAT AGACAGTGAT ACCGATGGAG AGGACGAGGG GATCCCTGCT 
ACCCCAGCAG TGGTTCCCAT GAAGGAGAGG CAGATCTTCC ACGAAGCTGG TACACAGAGC 
CCCCAGGCAC CTGGTGTGGC ACGTTGGCAG GAGAGCCCAG CTGATGGTGA TACAGATATA 
GAGGAGGGGG AGGCCCCCCC AGACAGAAGC CAAGCCTCCG TGGTGATCGA CAGCAATACA 
GACGATGAGG AAGAAGTCTC AGCAGCACTG ACACTGGCAT GTCTAAGAGA GAGCCAGGCT 
GGTAAGTGGA ACCGAGATCC AGATGCAGAA GAGACCAGGG CCCAACCCGT GGCCCTTCTG 
GAGCGAAGCC AGGCCTCTGC TGGGGGAGAC AGTGACACAG ATGTGGAGGA AGAGGGGCTC 
CCAGTGGAAA GGAGGGGAAT GGTTCCCAAG GGTTACATGG ACAGGGAATA TTCAAAAAAG 
AGCCAGCACC CTCCCAGGGA CAGTGATACA GAGGGGAAGG AAGATGAGAG CTCACCGGGG 
GTCCACCTGG AGAGAAGCCA GGCCTCTGCA CAAGTGGAGG ATGAGGTCCC ACTGGGGCCA 
GCTGTTGCAC TTCCGGAGAA GCGTCAGGTA CAAGGCATAG TGTGGCCACA TCACACCGAT 
GCGGAGGCAG AAGGGGACCC GGCACAGCTC CCCGTGCTGC GTCTAGAGGA AGCCCGGCCT 
CCTCTAGCTG GGGACTGTGA ACTGGATTCG GAGAACACAT CTGCTGTCAG AAAGAGCCAG 
CTTCCAGCGG AAAAAGATGC TGGGACCACG TGGGCTGCAG CCGTTCCTGA ACAGGACAGA 
GCACTTGCCA TCGGGACCCA GGGTGGGTCA TCCACGGCAC CAGGGGAGCA AGACCTTCTC 
CCGGTCTCAA GGGAAGACCT AGCAGATATG GTGGTGGACA TAGGCACTCC AGGGGAGCCC 
CAACCGCAGA GAGAGGGGGC CCAGACCACC ACAGGAAGGG AGAGAGAACC ATATGGGAAT 
AGGGCCACAG ACTCTGGAGA CAACCTCCAC GATTCTGAAG ATCTGGACCT ACCAGCTACC 
CAGTGCTTTG TAGACAGAAA GAATCAGAGC CTGGAAGCAG CCCCCAGCAT GGAGGATGAG 
CCCACCCAGG CCTTCCTGTT TCCTCTGCCC CAAGAGCCTG GCCCTTCCTG TTGCAGCTCC 
CAGGCCACAG GTTCCTTGGA TGAGTCATGG GAGGCCTTGG CGACACAGCC ATTCTGTCCG 
AGAGAATCTG AGGCCTCCGA GACCCAAACC GTTGTCACCC TCCTTGACAC CCATGCATCT 
TGGCCCTCTC CATCTAGGAC AGCACAGCAA GAGCAACATC CAGAGAGCCC AGTCCATGCA 
GAGCCACTGG GGATGGAAGG CAGAGAGATG CAGACTGTGG AGAAAGAAAT GGGTACCCCA 
AGAGAAACAG CAGAGAGGGT GATCCCTGAG GGAGGGCCAC CGCAGGAGGA AACCAAGAAA 
CTGCCCTCAG AAGGAGAGAG GGAAGATGTG ACGGGAGAGG AAGAATTAAT CCGGGGGATA 
CAGGGCAGTG AAAAAAATCA GGTGTTTGCT AGAGATACTC AGAGCCAAGA ATCTGACAAA 
AAAGTGAAAA GTGCAAGTAC TGGAAGGGGA ATGGAGATTT TAAAGGTAGA AATTGAGACA 
CCCAAGGAAA CACAAGAGAA AGAGAGAGAA AAGCAGACTC TCGCAGGGGA AATATTTGAG 
AGTGAAGCAG GGAAACTGGT AGTAGAGAGA GAGAGTGAGG CAGATGGGTT AGAAGTCAAG 
GAACCCCAAG AGCTACTGGA CAGAAGCCCA CAGATGGGGG AGACAGAGGC GGGGGACCAG 
GACCAGAAAG GCCAAGCCTC TGGTCTGCCA TCAGAGCCTG GAGCAGGGGC AGGAGACCTT 
CAGGGACTTG CTTCAGACCC AGTAGCTTCT GGGAGCCAGG CAGGTGGAGG AAGGGGAGCC 
CCAGGGAGCC CCAGGAGGCA GCAGAGAGGT GACTTGAATT GCGAGATGCC ACCTGCTCAG 
AAGGCTTCCA GGGGTGATCA GGAATCCCCA GATGCTTGTC TGCCTCCTGC AGCGCCTGAA 
GCCTCAGCCG CACTCCCAAA TTCCCTCATC ATCTCTCAAA TCCAAAAACA TCCCGCACCT 
CAGTCCCTCC TTTTTCCCTC TCTAGCTCCT TTAGAACTGC CTATTCCCAG GACCAGACAA 
AATGAGAGTC AGGAAGCTCC AGAGACTCCC TTCTCCTCAG AGCTGAACTC TGTCCACCCA 
GAACCCAAAG TCAGGCTCCA GGCGTCCTCT CCAGTTTCTT CTCTACCCCT TGAGCCCCAC 
CCTACCACCC CCACAGGCCA GCCTATTGCC CTTGAACCCA TATCTGGGGT CTCTCGGAGC 
AGGACACGTA GTTCCTTTGA TGTAACTGCC TCATCAGTTG TCCCCACAGC CGTTGCACTG 
CAGCCATCCA CCTCCACAGA CAAGCCTGTC ACCCCTAAGC CCACACTTCG GGCCCCTCGG 
GGCAGGGCAC AGAGGTCTTC TGTCAAAACC CCTGAACCCA ATGTCACCAC AGACCAGCCT 
GTTGCCCCTG AGCTCACAGC TATGGCAACT CGGGGCAGGG CACAGAGGTC TTCTGTCAAG 
ATTCCCAAAC CAGATAACCC CACAACACCC AAGCCCCAGC CTTCCACTTC CACAGACCAG 
CCTGTCATCC CCAAACCCAC ATCTCGGGCC CCTCGGGGCA GGACACCTAG GTCTTCTGCC 
AAGACTCCTG AACCAGCTGT CCCCACAGCC TCTGAGCTCC AGCCTGCTGC CCCTAAAGAC 
CAGCCTGTTG CTCCTGAGCT CACATCTAGA GCCACTCGGG GCAAGACACA GAGGTCTATC 
AAGACTTCCA AACCAGATAT GCCCACAACT CCCGAGCCCC AGCCTTCCAC CTCCACAGAC 
CAGCCTGTCA CCCCCAAACC CACGTCTCGG GCCCCTCGGG GCAGGACACC TAGGTCTTCC 
GCCAAGACTC CTGAGCCAGT TGTCTCCACA GCCTCTGAGC TCCAGCCTTC TGCCCTCACA 
GATGAGCCTG TCACTCCTGA GCTCACATCT AGGGCTACTC GGGGTCGGGC ACAGAGGTCC 
TCTGTCAAAA CCCCTGATCC AGTTACCACC ACAACACCTG AGCTCCAGCC TTCCACCTCC 
ACAGACCAGC TTGTCACCCC CAAACGCACA TCTCGGGCCC CTCGAGGCAG GACACGTAGG 
TTGTCTGCCA AGACTCCTGA ACCAGTTGTT CCCACAGCCT CTGAGCTCCA GCCTTCTGCC 
CCTGCAGACC AGCCTGTTGG TCCTTGGGCC ACTCAGTGTA GAAGACATAG GTCTTCTGTC 
AAGACCCCAG AACCAGTTGT CCCCACAGTC CCTGAACCTC AGCCTTCCAC TTCTAAAGAC 
CAGTCTGTCA CTCCTGAGCC CACATCTCAG GCCACTGAGA GCCAAACACA TAGGTCCTCT 
ATCAAGACAT CCCAGCCAAC GGAACCCACA GCCTCTGACC TCAAACCTTC CTCCCCCACA 
GACCAGCTTG TCACTCCCAA GGTCATAGCT CAGGGTGGTC CAAGCAGGAC AGGAAAGGCT 
TCCACAGCAA GTGCTGTGCT GGTTCCTACT ACCCCTGAAT TCCAGTCTCC AGTCCCGTCA 
GAACAGCCTC TTCCCCCTGA CCCCATCCCT GAAGTCAACT GCAGCAGGAG GCTGAGGGCC 
ACTAGGAAAC ATGGGTCTCC CACAGCTCAT GTTCATGAGC CCTGCACCGT ACCCCCTGAA 
CCTAACTCCC ACTCTTCAAG GAACCAAAGA CATGGGGCAG TGAAAGCAGC CAAGCCCCTT 
AGCACCATTC CTGAGCCTGC CTTTGCCCAG CTCCCCGAGG CACCGCCTCA CACTCCCCAG 
GTGCCAAAGG AGGAGGCAGC AGATGGATCA GGCTTCACCC CAGAGCCCCA GCCTAGGGCC 
TCTCAAAACC ACAAGAGGCC TTCAGCTACT GCACATTCAC CTCCACTTCA AAAACGGCTC 
CAGAGAGGGA GAGTCCCTCA GAAGGCAGCA TCCCTTAAGG AAGAAGAAAA TCCAGTAGCG 
AGGCCGAGGA AGGAAGAGGG TGTAGTGATT CCAGGTCCAG GCAAGAGAAA GAGAGAGCAG 
ACAGAGGAGG AGTCCCAGGG AAGACCGAGC CGCAGCCTGC GACGGACCAA ACCGATGCAG 
GAGTCCACGG CCCCCAAAGT GCTGTTCACC GGTGTGGTGG ATGCTCGCGG AGAGAGGACG 
GTGCTGGCCC TGGGGGGCAG TTTGGCCAGC TCGGTGGCCG AGGCTTCTCA CCTGGTGACT 
GATCGGATCC GCCGGACGGT CAAGTTTCTG TGTGCCCTGG GCCGGGGCAT CCCCATCCTC 
TCCCTGGCCT GGCTGCATGA GTCCCGCAAG GCAGGCTGCT TCTTGCCCCC GGACGAATAT 
TTGGTGACTG ATCCTGAGCA GGAGAAGAAC TTCGGCTTCA GCCTTCGGGA GGCCCTGAGC 
CGAGCTCAGG AGCGAAGGCT GCTGGAGGGC TATGAGATTC ACGTGACCCC CGGAGTCCAG 
CCACCGCCAC CTCAGATGGG AGAAATCATC AGCTGCTGTG GAGGCGCCAT CCTGCCCAGC 
ATGCCCCGGT CCTACAAGCC TCAGAGGTTC GTGATCACAT GTTCCCAGGA CTTTCCTCGA 
TGTGCTGTTC CATATCGGGT TGGGCTGCCT ATCCTCTCAC CCGAATTCCT GCTGACGGGA 
GTACTGAAGC AGGAAGTCAA GCCAGAGGCC TTTGCCTTCT CCACTGTGGA AATGTCATCC 
ACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011956376.1
CDS	115..5880
Translation	MEDTQILNWEVEEEEEVAESPNESLGYSLEPLAQLHIFSSSYGPEKDFPLYLGKNMIGRMPDCSVALPFSSISKQHAVIEISAWDKAPVLRDCGSLNGTQILRPPKVLSPGVSHRLRDRELILFADLPCQYHRLDVPRPFVSRGPLSVEETPRVQGGTQPSRLLLAEDSEEEVDSLLDKCVVKGPRTSSSATVVPESDEEGPSPAPDGPGPPSAFNLNSDTDEEESQESGAGEASSAARRGTAAETEQPEPVTAEIQIEKDQCSVKERNGDTEIERDVRNGVVPTGVILERSQPSGEDSDTDVSDESGPRRRLVGVRPKRAWSCNFIDSDTDGEDEGIPATPAVVPMKERQIFHEAGTQSPQAPGVARWQESPADGDTDIEEGEAPPDRSQASVVIDSNTDDEEEVSAALTLACLRESQAGKWNRDPDAEETRAQPVALLERSQASAGGDSDTDVEEEGLPVERRGMVPKGYMDREYSKKSQHPPRDSDTEGKEDESSPGVHLERSQASAQVEDEVPLGPAVALPEKRQVQGIVWPHHTDAEAEGDPAQLPVLRLEEARPPLAGDCELDSENTSAVRKSQLPAEKDAGTTWAAAVPEQDRALAIGTQGGSSTAPGEQDLLPVSREDLADMVVDIGTPGEPQPQREGAQTTTGREREPYGNRATDSGDNLHDSEDLDLPATQCFVDRKNQSLEAAPSMEDEPTQAFLFPLPQEPGPSCCSSQATGSLDESWEALATQPFCPRESEASETQTVVTLLDTHASWPSPSRTAQQEQHPESPVHAEPLGMEGREMQTVEKEMGTPRETAERVIPEGGPPQEETKKLPSEGEREDVTGEEELIRGIQGSEKNQVFARDTQSQESDKKVKSASTGRGMEILKVEIETPKETQEKEREKQTLAGEIFESEAGKLVVERESEADGLEVKEPQELLDRSPQMGETEAGDQDQKGQASGLPSEPGAGAGDLQGLASDPVASGSQAGGGRGAPGSPRRQQRGDLNCEMPPAQKASRGDQESPDACLPPAAPEASAALPNSLIISQIQKHPAPQSLLFPSLAPLELPIPRTRQNESQEAPETPFSSELNSVHPEPKVRLQASSPVSSLPLEPHPTTPTGQPIALEPISGVSRSRTRSSFDVTASSVVPTAVALQPSTSTDKPVTPKPTLRAPRGRAQRSSVKTPEPNVTTDQPVAPELTAMATRGRAQRSSVKIPKPDNPTTPKPQPSTSTDQPVIPKPTSRAPRGRTPRSSAKTPEPAVPTASELQPAAPKDQPVAPELTSRATRGKTQRSIKTSKPDMPTTPEPQPSTSTDQPVTPKPTSRAPRGRTPRSSAKTPEPVVSTASELQPSALTDEPVTPELTSRATRGRAQRSSVKTPDPVTTTTPELQPSTSTDQLVTPKRTSRAPRGRTRRLSAKTPEPVVPTASELQPSAPADQPVGPWATQCRRHRSSVKTPEPVVPTVPEPQPSTSKDQSVTPEPTSQATESQTHRSSIKTSQPTEPTASDLKPSSPTDQLVTPKVIAQGGPSRTGKASTASAVLVPTTPEFQSPVPSEQPLPPDPIPEVNCSRRLRATRKHGSPTAHVHEPCTVPPEPNSHSSRNQRHGAVKAAKPLSTIPEPAFAQLPEAPPHTPQVPKEEAADGSGFTPEPQPRASQNHKRPSATAHSPPLQKRLQRGRVPQKAASLKEEENPVARPRKEEGVVIPGPGKRKREQTEEESQGRPSRSLRRTKPMQESTAPKVLFTGVVDARGERTVLALGGSLASSVAEASHLVTDRIRRTVKFLCALGRGIPILSLAWLHESRKAGCFLPPDEYLVTDPEQEKNFGFSLREALSRAQERRLLEGYEIHVTPGVQPPPPQMGEIISCCGGAILPSMPRSYKPQRFVITCSQDFPRCAVPYRVGLPILSPEFLLTGVLKQEVKPEAFAFSTVEMSST

Target ORF information:

RefSeq Version	XM_012100986.2
Organism	Ovis aries(sheep)
Definition	Ovis aries mediator of DNA damage checkpoint 1 (MDC1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012100986.2

ORF Insert Sequence:

ATGGAAGACA CCCAGATTTT AAACTGGGAA GTTGAAGAAG AGGAGGAGGT TGCAGAGAGC 
CCCAATGAAT CTCTGGGGTA TAGCTTGGAG CCCCTAGCAC AGCTGCATAT CTTCAGTAGT 
TCCTATGGAC CAGAAAAAGA CTTTCCACTC TACCTTGGAA AGAACATGAT AGGCCGAATG 
CCTGATTGCT CTGTGGCCCT GCCCTTTTCA TCAATATCCA AACAACATGC AGTGATTGAA 
ATCTCTGCCT GGGACAAGGC GCCTGTCCTT CGGGATTGCG GGAGCCTCAA TGGCACTCAA 
ATCCTGCGGC CTCCTAAGGT CCTGAGCCCT GGGGTGAGTC ATCGTTTGCG GGACCGGGAG 
TTGATTCTCT TTGCTGACTT GCCCTGCCAG TACCATCGCT TGGATGTCCC CCGGCCTTTT 
GTCTCCCGGG GCCCTCTAAG TGTAGAGGAG ACACCCAGGG TACAGGGAGG AACTCAACCC 
TCCAGGCTTC TGTTGGCTGA GGACTCAGAG GAAGAAGTAG ATTCCCTTTT GGACAAGTGT 
GTGGTGAAAG GACCAAGGAC CTCCTCTTCG GCAACAGTCG TTCCAGAGAG TGATGAAGAA 
GGGCCTTCCC CTGCCCCAGA TGGGCCCGGG CCACCTTCTG CCTTCAACTT GAACAGCGAC 
ACGGATGAGG AAGAAAGTCA GGAATCAGGA GCAGGGGAGG CCTCTTCAGC TGCCAGAAGA 
GGTACCGCTG CAGAGACGGA ACAGCCTGAA CCTGTCACAG CCGAGATCCA GATTGAGAAG 
GATCAGTGTT CCGTGAAGGA GAGGAACGGG GACACAGAAA TCGAGAGGGA TGTGAGGAAT 
GGGGTGGTTC CGACTGGAGT GATTCTGGAG AGGAGCCAGC CTTCTGGGGA GGACAGTGAC 
ACAGATGTGA GTGATGAGAG TGGGCCTCGA AGAAGGCTGG TTGGAGTCCG TCCGAAAAGG 
GCCTGGTCTT GTAACTTCAT AGACAGTGAT ACCGATGGAG AGGACGAGGG GATCCCTGCT 
ACCCCAGCAG TGGTTCCCAT GAAGGAGAGG CAGATCTTCC ACGAAGCTGG TACACAGAGC 
CCCCAGGCAC CTGGTGTGGC ACGTTGGCAG GAGAGCCCAG CTGATGGTGA TACAGATATA 
GAGGAGGGGG AGGCCCCCCC AGACAGAAGC CAAGCCTCCG TGGTGATCGA CAGCAATACA 
GACGATGAGG AAGAAGTCTC AGCAGCACTG ACACTGGCAT GTCTAAGAGA GAGCCAGGCT 
GGTAAGTGGA ACCGAGATCC AGATGCAGAA GAGACCAGGG CCCAACCCGT GGCCCTTCTG 
GAGCGAAGCC AGGCCTCTGC TGGGGGAGAC AGTGACACAG ATGTGGAGGA AGAGGGGCTC 
CCAGTGGAAA GGAGGGGAAT GGTTCCCAAG GGTTACATGG ACAGGGAATA TTCAAAAAAG 
AGCCAGCACC CTCCCAGGGA CAGTGATACA GAGGGGAAGG AAGATGAGAG CTCACCGGGG 
GTCCACCTGG AGAGAAGCCA GGCCTCTGCA CAAGTGGAGG ATGAGGTCCC ACTGGGGCCA 
GCTGTTGCAC TTCCGGAGAA GCGTCAGGTA CAAGGCATAG TGTGGCCACA TCACACCGAT 
GCGGAGGCAG AAGGGGACCC GGCACAGCTC CCCGTGCTGC GTCTAGAGGA AGCCCGGCCT 
CCTCTAGCTG GGGACTGTGA ACTGGATTCG GAGAACACAT CTGCTGTCAG AAAGAGCCAG 
CTTCCAGCGG AAAAAGATGC TGGGACCACG TGGGCTGCAG CCGTTCCTGA ACAGGACAGA 
GCACTTGCCA TCGGGACCCA GGGTGGGTCA TCCACGGCAC CAGGGGAGCA AGACCTTCTC 
CCGGTCTCAA GGGAAGACCT AGCAGATATG GTGGTGGACA TAGGCACTCC AGGGGAGCCC 
CAACCGCAGA GAGAGGGGGC CCAGACCACC ACAGGAAGGG AGAGAGAACC ATATGGGAAT 
AGGGCCACAG ACTCTGGAGA CAACCTCCAC GATTCTGAAG ATCTGGACCT ACCAGCTACC 
CAGTGCTTTG TAGACAGAAA GAATCAGAGC CTGGAAGCAG CCCCCAGCAT GGAGGATGAG 
CCCACCCAGG CCTTCCTGTT TCCTCTGCCC CAAGAGCCTG GCCCTTCCTG TTGCAGCTCC 
CAGGCCACAG GTTCCTTGGA TGAGTCATGG GAGGCCTTGG CGACACAGCC ATTCTGTCCG 
AGAGAATCTG AGGCCTCCGA GACCCAAACC GTTGTCACCC TCCTTGACAC CCATGCATCT 
TGGCCCTCTC CATCTAGGAC AGCACAGCAA GAGCAACATC CAGAGAGCCC AGTCCATGCA 
GAGCCACTGG GGATGGAAGG CAGAGAGATG CAGACTGTGG AGAAAGAAAT GGGTACCCCA 
AGAGAAACAG CAGAGAGGGT GATCCCTGAG GGAGGGCCAC CGCAGGAGGA AACCAAGAAA 
CTGCCCTCAG AAGGAGAGAG GGAAGATGTG ACGGGAGAGG AAGAATTAAT CCGGGGGATA 
CAGGGCAGTG AAAAAAATCA GGTGTTTGCT AGAGATACTC AGAGCCAAGA ATCTGACAAA 
AAAGTGAAAA GTGCAAGTAC TGGAAGGGGA ATGGAGATTT TAAAGGTAGA AATTGAGACA 
CCCAAGGAAA CACAAGAGAA AGAGAGAGAA AAGCAGACTC TCGCAGGGGA AATATTTGAG 
AGTGAAGCAG GGAAACTGGT AGTAGAGAGA GAGAGTGAGG CAGATGGGTT AGAAGTCAAG 
GAACCCCAAG AGCTACTGGA CAGAAGCCCA CAGATGGGGG AGACAGAGGC GGGGGACCAG 
GACCAGAAAG GCCAAGCCTC TGGTCTGCCA TCAGAGCCTG GAGCAGGGGC AGGAGACCTT 
CAGGGACTTG CTTCAGACCC AGTAGCTTCT GGGAGCCAGG CAGGTGGAGG AAGGGGAGCC 
CCAGGGAGCC CCAGGAGGCA GCAGAGAGGT GACTTGAATT GCGAGATGCC ACCTGCTCAG 
AAGGCTTCCA GGGGTGATCA GGAATCCCCA GATGCTTGTC TGCCTCCTGC AGCGCCTGAA 
GCCTCAGCCG CACTCCCAAA TTCCCTCATC ATCTCTCAAA TCCAAAAACA TCCCGCACCT 
CAGTCCCTCC TTTTTCCCTC TCTAGCTCCT TTAGAACTGC CTATTCCCAG GACCAGACAA 
AATGAGAGTC AGGAAGCTCC AGAGACTCCC TTCTCCTCAG AGCTGAACTC TGTCCACCCA 
GAACCCAAAG TCAGGCTCCA GGCGTCCTCT CCAGTTTCTT CTCTACCCCT TGAGCCCCAC 
CCTACCACCC CCACAGGCCA GCCTATTGCC CTTGAACCCA TATCTGGGGT CTCTCGGAGC 
AGGACACGTA GTTCCTTTGA TGTAACTGCC TCATCAGTTG TCCCCACAGC CGTTGCACTG 
CAGCCATCCA CCTCCACAGA CAAGCCTGTC ACCCCTAAGC CCACACTTCG GGCCCCTCGG 
GGCAGGGCAC AGAGGTCTTC TGTCAAAACC CCTGAACCCA ATGTCACCAC AGACCAGCCT 
GTTGCCCCTG AGCTCACAGC TATGGCAACT CGGGGCAGGG CACAGAGGTC TTCTGTCAAG 
ATTCCCAAAC CAGATAACCC CACAACACCC AAGCCCCAGC CTTCCACTTC CACAGACCAG 
CCTGTCATCC CCAAACCCAC ATCTCGGGCC CCTCGGGGCA GGACACCTAG GTCTTCTGCC 
AAGACTCCTG AACCAGCTGT CCCCACAGCC TCTGAGCTCC AGCCTGCTGC CCCTAAAGAC 
CAGCCTGTTG CTCCTGAGCT CACATCTAGA GCCACTCGGG GCAAGACACA GAGGTCTATC 
AAGACTTCCA AACCAGATAT GCCCACAACT CCCGAGCCCC AGCCTTCCAC CTCCACAGAC 
CAGCCTGTCA CCCCCAAACC CACGTCTCGG GCCCCTCGGG GCAGGACACC TAGGTCTTCC 
GCCAAGACTC CTGAGCCAGT TGTCTCCACA GCCTCTGAGC TCCAGCCTTC TGCCCTCACA 
GATGAGCCTG TCACTCCTGA GCTCACATCT AGGGCTACTC GGGGTCGGGC ACAGAGGTCC 
TCTGTCAAAA CCCCTGATCC AGTTACCACC ACAACACCTG AGCTCCAGCC TTCCACCTCC 
ACAGACCAGC TTGTCACCCC CAAACGCACA TCTCGGGCCC CTCGAGGCAG GACACGTAGG 
TTGTCTGCCA AGACTCCTGA ACCAGTTGTT CCCACAGCCT CTGAGCTCCA GCCTTCTGCC 
CCTGCAGACC AGCCTGTTGG TCCTTGGGCC ACTCAGTGTA GAAGACATAG GTCTTCTGTC 
AAGACCCCAG AACCAGTTGT CCCCACAGTC CCTGAACCTC AGCCTTCCAC TTCTAAAGAC 
CAGTCTGTCA CTCCTGAGCC CACATCTCAG GCCACTGAGA GCCAAACACA TAGGTCCTCT 
ATCAAGACAT CCCAGCCAAC GGAACCCACA GCCTCTGACC TCAAACCTTC CTCCCCCACA 
GACCAGCTTG TCACTCCCAA GGTCATAGCT CAGGGTGGTC CAAGCAGGAC AGGAAAGGCT 
TCCACAGCAA GTGCTGTGCT GGTTCCTACT ACCCCTGAAT TCCAGTCTCC AGTCCCGTCA 
GAACAGCCTC TTCCCCCTGA CCCCATCCCT GAAGTCAACT GCAGCAGGAG GCTGAGGGCC 
ACTAGGAAAC ATGGGTCTCC CACAGCTCAT GTTCATGAGC CCTGCACCGT ACCCCCTGAA 
CCTAACTCCC ACTCTTCAAG GAACCAAAGA CATGGGGCAG TGAAAGCAGC CAAGCCCCTT 
AGCACCATTC CTGAGCCTGC CTTTGCCCAG CTCCCCGAGG CACCGCCTCA CACTCCCCAG 
GTGCCAAAGG AGGAGGCAGC AGATGGATCA GGCTTCACCC CAGAGCCCCA GCCTAGGGCC 
TCTCAAAACC ACAAGAGGCC TTCAGCTACT GCACATTCAC CTCCACTTCA AAAACGGCTC 
CAGAGAGGGA GAGTCCCTCA GAAGGCAGCA TCCCTTAAGG AAGAAGAAAA TCCAGTAGCG 
AGGCCGAGGA AGGAAGAGGG TGTAGTGATT CCAGGTCCAG GCAAGAGAAA GAGAGAGCAG 
ACAGAGGAGG AGTCCCAGGG AAGACCGAGC CGCAGCCTGC GACGGACCAA ACCGATGCAG 
GAGTCCACGG CCCCCAAAGT GCTGTTCACC GGTGTGGTGG ATGCTCGCGG AGAGAGGACG 
GTGCTGGCCC TGGGGGGCAG TTTGGCCAGC TCGGTGGCCG AGGCTTCTCA CCTGGTGACT 
GATCGGATCC GCCGGACGGT CAAGTTTCTG TGTGCCCTGG GCCGGGGCAT CCCCATCCTC 
TCCCTGGCCT GGCTGCATGA GTCCCGCAAG GCAGGCTGCT TCTTGCCCCC GGACGAATAT 
TTGGTGACTG ATCCTGAGCA GGAGAAGAAC TTCGGCTTCA GCCTTCGGGA GGCCCTGAGC 
CGAGCTCAGG AGCGAAGGCT GCTGGAGGGC TATGAGATTC ACGTGACCCC CGGAGTCCAG 
CCACCGCCAC CTCAGATGGG AGAAATCATC AGCTGCTGTG GAGGCGCCAT CCTGCCCAGC 
ATGCCCCGGT CCTACAAGCC TCAGAGGTTC GTGATCACAT GTTCCCAGGA CTTTCCTCGA 
TGTGCTGTTC CATATCGGGT TGGGCTGCCT ATCCTCTCAC CCGAATTCCT GCTGACGGGA 
GTACTGAAGC AGGAAGTCAA GCCAGAGGCC TTTGCCTTCT CCACTGTGGA AATGTCATCC 
ACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MDC1 ( XM_012100987.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_011956377.1 Ovis aries mediator of DNA damage checkpoint 1 (MDC1), transcript variant X4, mRNA.

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CloneID	OSm28028
Clone ID Related Accession (Same CDS sequence)	XM_012100987.2
Accession Version	XM_012100987.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5718bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries(sheep)
Product	mediator of DNA damage checkpoint protein 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014639029.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012100987.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGTGT CCCACTTTCC TTGTCTACAT TTTCCCTTTA AGATCATGGA AGACACCCAG 
ATTTTAAACT GGGAAGTTGA AGAAGAGGAG GAGGTTGCAG AGAGCCCCAA TGAATCTCTG 
GGGTATAGCT TGGAGCCCCT AGCACAGCTG CATATCTTCA GTAGTTCCTA TGGACCAGAA 
AAAGACTTTC CACTCTACCT TGGAAAGAAC ATGATAGGCC GAATGCCTGA TTGCTCTGTG 
GCCCTGCCCT TTTCATCAAT ATCCAAACAA CATGCAGTGA TTGAAATCTC TGCCTGGGAC 
AAGGCGCCTG TCCTTCGGGA TTGCGGGAGC CTCAATGGCA CTCAAATCCT GCGGCCTCCT 
AAGGTCCTGA GCCCTGGGGT GAGTCATCGT TTGCGGGACC GGGAGTTGAT TCTCTTTGCT 
GACTTGCCCT GCCAGTACCA TCGCTTGGAT GTCCCCCGGC CTTTTGTCTC CCGGGGCCCT 
CTAAGTGTAG AGGAGACACC CAGGGTACAG GGAGGAACTC AACCCTCCAG GCTTCTGTTG 
GCTGAGGACT CAGAGGAAGA AGTAGATTCC CTTTTGGACA AGTGTGTGGT GAAAGGACCA 
AGGACCTCCT CTTCGGCAAC AGTCGTTCCA GAGAGTGATG AAGAAGGGCC TTCCCCTGCC 
CCAGATGGGC CCGGGCCACC TTCTGCCTTC AACTTGAACA GCGACACGGA TGAGGAAGAA 
AGTCAGGAAT CAGGAGCAGG GGAGGCCTCT TCAGCTGCCA GAAGAGGTAC CGCTGCAGAG 
ACGGAACAGC CTGAACCTGT CACAGCCGAG ATCCAGATTG AGAAGGATCA GTGTTCCGTG 
AAGGAGAGGA ACGGGGACAC AGAAATCGAG AGGGATGTGA GGAATGGGGT GGTTCCGACT 
GGAGTGATTC TGGAGAGGAG CCAGCCTTCT GGGGAGGACA GTGACACAGA TGTGAGTGAT 
GAGAGTGGGC CTCGAAGAAG GCTGGTTGGA GTCCGTCCGA AAAGGGCCTG GTCTTGTAAC 
TTCATAGACA GTGATACCGA TGGAGAGGAC GAGGGGATCC CTGCTACCCC AGCAGTGGTT 
CCCATGAAGG AGAGGCAGAT CTTCCACGAA GCTGGTACAC AGAGCCCCCA GGCACCTGGT 
GTGGCACGTT GGCAGGAGAG CCCAGCTGAT GGTGATACAG ATATAGAGGA GGGGGAGGCC 
CCCCCAGACA GAAGCCAAGC CTCCGTGGTG ATCGACAGCA ATACAGACGA TGAGGAAGAA 
GTCTCAGCAG CACTGACACT GGCATGTCTA AGAGAGAGCC AGGCTGGTAA GTGGAACCGA 
GATCCAGATG CAGAAGAGAC CAGGGCCCAA CCCGTGGCCC TTCTGGAGCG AAGCCAGGCC 
TCTGCTGGGG GAGACAGTGA CACAGATGTG GAGGAAGAGG GGCTCCCAGT GGAAAGGAGG 
GGAATGGTTC CCAAGGGTTA CATGGACAGG GAATATTCAA AAAAGAGCCA GCACCCTCCC 
AGGGACAGTG ATACAGAGGG GAAGGAAGAT GAGAGCTCAC CGGGGGTCCA CCTGGAGAGA 
AGCCAGGCCT CTGCACAAGT GGAGGATGAG GTCCCACTGG GGCCAGCTGT TGCACTTCCG 
GAGAAGCGTC AGGTACAAGG CATAGTGTGG CCACATCACA CCGATGCGGA GGCAGAAGGG 
GACCCGGCAC AGCTCCCCGT GCTGCGTCTA GAGGAAGCCC GGCCTCCTCT AGCTGGGGAC 
TGTGAACTGG ATTCGGAGAA CACATCTGCT GTCAGAAAGA GCCAGCTTCC AGCGGAAAAA 
GATGCTGGGA CCACGTGGGC TGCAGCCGTT CCTGAACAGG ACAGAGCACT TGCCATCGGG 
ACCCAGGGTG GGTCATCCAC GGCACCAGGG GAGCAAGACC TTCTCCCGGT CTCAAGGGAA 
GACCTAGCAG ATATGGTGGT GGACATAGGC ACTCCAGGGG AGCCCCAACC GCAGAGAGAG 
GGGGCCCAGA CCACCACAGG AAGGGAGAGA GAACCATATG GGAATAGGGC CACAGACTCT 
GGAGACAACC TCCACGATTC TGAAGATCTG GACCTACCAG CTACCCAGTG CTTTGTAGAC 
AGAAAGAATC AGAGCCTGGA AGGTTCCTTG GATGAGTCAT GGGAGGCCTT GGCGACACAG 
CCATTCTGTC CGAGAGAATC TGAGGCCTCC GAGACCCAAA CCGTTGTCAC CCTCCTTGAC 
ACCCATGCAT CTTGGCCCTC TCCATCTAGG ACAGCACAGC AAGAGCAACA TCCAGAGAGC 
CCAGTCCATG CAGAGCCACT GGGGATGGAA GGCAGAGAGA TGCAGACTGT GGAGAAAGAA 
ATGGGTACCC CAAGAGAAAC AGCAGAGAGG GTGATCCCTG AGGGAGGGCC ACCGCAGGAG 
GAAACCAAGA AACTGCCCTC AGAAGGAGAG AGGGAAGATG TGACGGGAGA GGAAGAATTA 
ATCCGGGGGA TACAGGGCAG TGAAAAAAAT CAGGTGTTTG CTAGAGATAC TCAGAGCCAA 
GAATCTGACA AAAAAGTGAA AAGTGCAAGT ACTGGAAGGG GAATGGAGAT TTTAAAGGTA 
GAAATTGAGA CACCCAAGGA AACACAAGAG AAAGAGAGAG AAAAGCAGAC TCTCGCAGGG 
GAAATATTTG AGAGTGAAGC AGGGAAACTG GTAGTAGAGA GAGAGAGTGA GGCAGATGGG 
TTAGAAGTCA AGGAACCCCA AGAGCTACTG GACAGAAGCC CACAGATGGG GGAGACAGAG 
GCGGGGGACC AGGACCAGAA AGGCCAAGCC TCTGGTCTGC CATCAGAGCC TGGAGCAGGG 
GCAGGAGACC TTCAGGGACT TGCTTCAGAC CCAGTAGCTT CTGGGAGCCA GGCAGGTGGA 
GGAAGGGGAG CCCCAGGGAG CCCCAGGAGG CAGCAGAGAG GTGACTTGAA TTGCGAGATG 
CCACCTGCTC AGAAGGCTTC CAGGGGTGAT CAGGAATCCC CAGATGCTTG TCTGCCTCCT 
GCAGCGCCTG AAGCCTCAGC CGCACTCCCA AATTCCCTCA TCATCTCTCA AATCCAAAAA 
CATCCCGCAC CTCAGTCCCT CCTTTTTCCC TCTCTAGCTC CTTTAGAACT GCCTATTCCC 
AGGACCAGAC AAAATGAGAG TCAGGAAGCT CCAGAGACTC CCTTCTCCTC AGAGCTGAAC 
TCTGTCCACC CAGAACCCAA AGTCAGGCTC CAGGCGTCCT CTCCAGTTTC TTCTCTACCC 
CTTGAGCCCC ACCCTACCAC CCCCACAGGC CAGCCTATTG CCCTTGAACC CATATCTGGG 
GTCTCTCGGA GCAGGACACG TAGTTCCTTT GATGTAACTG CCTCATCAGT TGTCCCCACA 
GCCGTTGCAC TGCAGCCATC CACCTCCACA GACAAGCCTG TCACCCCTAA GCCCACACTT 
CGGGCCCCTC GGGGCAGGGC ACAGAGGTCT TCTGTCAAAA CCCCTGAACC CAATGTCACC 
ACAGACCAGC CTGTTGCCCC TGAGCTCACA GCTATGGCAA CTCGGGGCAG GGCACAGAGG 
TCTTCTGTCA AGATTCCCAA ACCAGATAAC CCCACAACAC CCAAGCCCCA GCCTTCCACT 
TCCACAGACC AGCCTGTCAT CCCCAAACCC ACATCTCGGG CCCCTCGGGG CAGGACACCT 
AGGTCTTCTG CCAAGACTCC TGAACCAGCT GTCCCCACAG CCTCTGAGCT CCAGCCTGCT 
GCCCCTAAAG ACCAGCCTGT TGCTCCTGAG CTCACATCTA GAGCCACTCG GGGCAAGACA 
CAGAGGTCTA TCAAGACTTC CAAACCAGAT ATGCCCACAA CTCCCGAGCC CCAGCCTTCC 
ACCTCCACAG ACCAGCCTGT CACCCCCAAA CCCACGTCTC GGGCCCCTCG GGGCAGGACA 
CCTAGGTCTT CCGCCAAGAC TCCTGAGCCA GTTGTCTCCA CAGCCTCTGA GCTCCAGCCT 
TCTGCCCTCA CAGATGAGCC TGTCACTCCT GAGCTCACAT CTAGGGCTAC TCGGGGTCGG 
GCACAGAGGT CCTCTGTCAA AACCCCTGAT CCAGTTACCA CCACAACACC TGAGCTCCAG 
CCTTCCACCT CCACAGACCA GCTTGTCACC CCCAAACGCA CATCTCGGGC CCCTCGAGGC 
AGGACACGTA GGTTGTCTGC CAAGACTCCT GAACCAGTTG TTCCCACAGC CTCTGAGCTC 
CAGCCTTCTG CCCCTGCAGA CCAGCCTGTT GGTCCTTGGG CCACTCAGTG TAGAAGACAT 
AGGTCTTCTG TCAAGACCCC AGAACCAGTT GTCCCCACAG TCCCTGAACC TCAGCCTTCC 
ACTTCTAAAG ACCAGTCTGT CACTCCTGAG CCCACATCTC AGGCCACTGA GAGCCAAACA 
CATAGGTCCT CTATCAAGAC ATCCCAGCCA ACGGAACCCA CAGCCTCTGA CCTCAAACCT 
TCCTCCCCCA CAGACCAGCT TGTCACTCCC AAGGTCATAG CTCAGGGTGG TCCAAGCAGG 
ACAGGAAAGG CTTCCACAGC AAGTGCTGTG CTGGTTCCTA CTACCCCTGA ATTCCAGTCT 
CCAGTCCCGT CAGAACAGCC TCTTCCCCCT GACCCCATCC CTGAAGTCAA CTGCAGCAGG 
AGGCTGAGGG CCACTAGGAA ACATGGGTCT CCCACAGCTC ATGTTCATGA GCCCTGCACC 
GTACCCCCTG AACCTAACTC CCACTCTTCA AGGAACCAAA GACATGGGGC AGTGAAAGCA 
GCCAAGCCCC TTAGCACCAT TCCTGAGCCT GCCTTTGCCC AGCTCCCCGA GGCACCGCCT 
CACACTCCCC AGGTGCCAAA GGAGGAGGCA GCAGATGGAT CAGGCTTCAC CCCAGAGCCC 
CAGCCTAGGG CCTCTCAAAA CCACAAGAGG CCTTCAGCTA CTGCACATTC ACCTCCACTT 
CAAAAACGGC TCCAGAGAGG GAGAGTCCCT CAGAAGGCAG CATCCCTTAA GGAAGAAGAA 
AATCCAGTAG CGAGGCCGAG GAAGGAAGAG GGTGTAGTGA TTCCAGGTCC AGGCAAGAGA 
AAGAGAGAGC AGACAGAGGA GGAGTCCCAG GGAAGACCGA GCCGCAGCCT GCGACGGACC 
AAACCGATGC AGGAGTCCAC GGCCCCCAAA GTGCTGTTCA CCGGTGTGGT GGATGCTCGC 
GGAGAGAGGA CGGTGCTGGC CCTGGGGGGC AGTTTGGCCA GCTCGGTGGC CGAGGCTTCT 
CACCTGGTGA CTGATCGGAT CCGCCGGACG GTCAAGTTTC TGTGTGCCCT GGGCCGGGGC 
ATCCCCATCC TCTCCCTGGC CTGGCTGCAT GAGTCCCGCA AGGCAGGCTG CTTCTTGCCC 
CCGGACGAAT ATTTGGTGAC TGATCCTGAG CAGGAGAAGA ACTTCGGCTT CAGCCTTCGG 
GAGGCCCTGA GCCGAGCTCA GGAGCGAAGG CTGCTGGAGG GCTATGAGAT TCACGTGACC 
CCCGGAGTCC AGCCACCGCC ACCTCAGATG GGAGAAATCA TCAGCTGCTG TGGAGGCGCC 
ATCCTGCCCA GCATGCCCCG GTCCTACAAG CCTCAGAGGT TCGTGATCAC ATGTTCCCAG 
GACTTTCCTC GATGTGCTGT TCCATATCGG GTTGGGCTGC CTATCCTCTC ACCCGAATTC 
CTGCTGACGG GAGTACTGAA GCAGGAAGTC AAGCCAGAGG CCTTTGCCTT CTCCACTGTG 
GAAATGTCAT CCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011956377.1
CDS	144..5861
Translation	MEVSHFPCLHFPFKIMEDTQILNWEVEEEEEVAESPNESLGYSLEPLAQLHIFSSSYGPEKDFPLYLGKNMIGRMPDCSVALPFSSISKQHAVIEISAWDKAPVLRDCGSLNGTQILRPPKVLSPGVSHRLRDRELILFADLPCQYHRLDVPRPFVSRGPLSVEETPRVQGGTQPSRLLLAEDSEEEVDSLLDKCVVKGPRTSSSATVVPESDEEGPSPAPDGPGPPSAFNLNSDTDEEESQESGAGEASSAARRGTAAETEQPEPVTAEIQIEKDQCSVKERNGDTEIERDVRNGVVPTGVILERSQPSGEDSDTDVSDESGPRRRLVGVRPKRAWSCNFIDSDTDGEDEGIPATPAVVPMKERQIFHEAGTQSPQAPGVARWQESPADGDTDIEEGEAPPDRSQASVVIDSNTDDEEEVSAALTLACLRESQAGKWNRDPDAEETRAQPVALLERSQASAGGDSDTDVEEEGLPVERRGMVPKGYMDREYSKKSQHPPRDSDTEGKEDESSPGVHLERSQASAQVEDEVPLGPAVALPEKRQVQGIVWPHHTDAEAEGDPAQLPVLRLEEARPPLAGDCELDSENTSAVRKSQLPAEKDAGTTWAAAVPEQDRALAIGTQGGSSTAPGEQDLLPVSREDLADMVVDIGTPGEPQPQREGAQTTTGREREPYGNRATDSGDNLHDSEDLDLPATQCFVDRKNQSLEGSLDESWEALATQPFCPRESEASETQTVVTLLDTHASWPSPSRTAQQEQHPESPVHAEPLGMEGREMQTVEKEMGTPRETAERVIPEGGPPQEETKKLPSEGEREDVTGEEELIRGIQGSEKNQVFARDTQSQESDKKVKSASTGRGMEILKVEIETPKETQEKEREKQTLAGEIFESEAGKLVVERESEADGLEVKEPQELLDRSPQMGETEAGDQDQKGQASGLPSEPGAGAGDLQGLASDPVASGSQAGGGRGAPGSPRRQQRGDLNCEMPPAQKASRGDQESPDACLPPAAPEASAALPNSLIISQIQKHPAPQSLLFPSLAPLELPIPRTRQNESQEAPETPFSSELNSVHPEPKVRLQASSPVSSLPLEPHPTTPTGQPIALEPISGVSRSRTRSSFDVTASSVVPTAVALQPSTSTDKPVTPKPTLRAPRGRAQRSSVKTPEPNVTTDQPVAPELTAMATRGRAQRSSVKIPKPDNPTTPKPQPSTSTDQPVIPKPTSRAPRGRTPRSSAKTPEPAVPTASELQPAAPKDQPVAPELTSRATRGKTQRSIKTSKPDMPTTPEPQPSTSTDQPVTPKPTSRAPRGRTPRSSAKTPEPVVSTASELQPSALTDEPVTPELTSRATRGRAQRSSVKTPDPVTTTTPELQPSTSTDQLVTPKRTSRAPRGRTRRLSAKTPEPVVPTASELQPSAPADQPVGPWATQCRRHRSSVKTPEPVVPTVPEPQPSTSKDQSVTPEPTSQATESQTHRSSIKTSQPTEPTASDLKPSSPTDQLVTPKVIAQGGPSRTGKASTASAVLVPTTPEFQSPVPSEQPLPPDPIPEVNCSRRLRATRKHGSPTAHVHEPCTVPPEPNSHSSRNQRHGAVKAAKPLSTIPEPAFAQLPEAPPHTPQVPKEEAADGSGFTPEPQPRASQNHKRPSATAHSPPLQKRLQRGRVPQKAASLKEEENPVARPRKEEGVVIPGPGKRKREQTEEESQGRPSRSLRRTKPMQESTAPKVLFTGVVDARGERTVLALGGSLASSVAEASHLVTDRIRRTVKFLCALGRGIPILSLAWLHESRKAGCFLPPDEYLVTDPEQEKNFGFSLREALSRAQERRLLEGYEIHVTPGVQPPPPQMGEIISCCGGAILPSMPRSYKPQRFVITCSQDFPRCAVPYRVGLPILSPEFLLTGVLKQEVKPEAFAFSTVEMSST

Target ORF information:

RefSeq Version	XM_012100987.2
Organism	Ovis aries(sheep)
Definition	Ovis aries mediator of DNA damage checkpoint 1 (MDC1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012100987.2

ORF Insert Sequence:

ATGGAGGTGT CCCACTTTCC TTGTCTACAT TTTCCCTTTA AGATCATGGA AGACACCCAG 
ATTTTAAACT GGGAAGTTGA AGAAGAGGAG GAGGTTGCAG AGAGCCCCAA TGAATCTCTG 
GGGTATAGCT TGGAGCCCCT AGCACAGCTG CATATCTTCA GTAGTTCCTA TGGACCAGAA 
AAAGACTTTC CACTCTACCT TGGAAAGAAC ATGATAGGCC GAATGCCTGA TTGCTCTGTG 
GCCCTGCCCT TTTCATCAAT ATCCAAACAA CATGCAGTGA TTGAAATCTC TGCCTGGGAC 
AAGGCGCCTG TCCTTCGGGA TTGCGGGAGC CTCAATGGCA CTCAAATCCT GCGGCCTCCT 
AAGGTCCTGA GCCCTGGGGT GAGTCATCGT TTGCGGGACC GGGAGTTGAT TCTCTTTGCT 
GACTTGCCCT GCCAGTACCA TCGCTTGGAT GTCCCCCGGC CTTTTGTCTC CCGGGGCCCT 
CTAAGTGTAG AGGAGACACC CAGGGTACAG GGAGGAACTC AACCCTCCAG GCTTCTGTTG 
GCTGAGGACT CAGAGGAAGA AGTAGATTCC CTTTTGGACA AGTGTGTGGT GAAAGGACCA 
AGGACCTCCT CTTCGGCAAC AGTCGTTCCA GAGAGTGATG AAGAAGGGCC TTCCCCTGCC 
CCAGATGGGC CCGGGCCACC TTCTGCCTTC AACTTGAACA GCGACACGGA TGAGGAAGAA 
AGTCAGGAAT CAGGAGCAGG GGAGGCCTCT TCAGCTGCCA GAAGAGGTAC CGCTGCAGAG 
ACGGAACAGC CTGAACCTGT CACAGCCGAG ATCCAGATTG AGAAGGATCA GTGTTCCGTG 
AAGGAGAGGA ACGGGGACAC AGAAATCGAG AGGGATGTGA GGAATGGGGT GGTTCCGACT 
GGAGTGATTC TGGAGAGGAG CCAGCCTTCT GGGGAGGACA GTGACACAGA TGTGAGTGAT 
GAGAGTGGGC CTCGAAGAAG GCTGGTTGGA GTCCGTCCGA AAAGGGCCTG GTCTTGTAAC 
TTCATAGACA GTGATACCGA TGGAGAGGAC GAGGGGATCC CTGCTACCCC AGCAGTGGTT 
CCCATGAAGG AGAGGCAGAT CTTCCACGAA GCTGGTACAC AGAGCCCCCA GGCACCTGGT 
GTGGCACGTT GGCAGGAGAG CCCAGCTGAT GGTGATACAG ATATAGAGGA GGGGGAGGCC 
CCCCCAGACA GAAGCCAAGC CTCCGTGGTG ATCGACAGCA ATACAGACGA TGAGGAAGAA 
GTCTCAGCAG CACTGACACT GGCATGTCTA AGAGAGAGCC AGGCTGGTAA GTGGAACCGA 
GATCCAGATG CAGAAGAGAC CAGGGCCCAA CCCGTGGCCC TTCTGGAGCG AAGCCAGGCC 
TCTGCTGGGG GAGACAGTGA CACAGATGTG GAGGAAGAGG GGCTCCCAGT GGAAAGGAGG 
GGAATGGTTC CCAAGGGTTA CATGGACAGG GAATATTCAA AAAAGAGCCA GCACCCTCCC 
AGGGACAGTG ATACAGAGGG GAAGGAAGAT GAGAGCTCAC CGGGGGTCCA CCTGGAGAGA 
AGCCAGGCCT CTGCACAAGT GGAGGATGAG GTCCCACTGG GGCCAGCTGT TGCACTTCCG 
GAGAAGCGTC AGGTACAAGG CATAGTGTGG CCACATCACA CCGATGCGGA GGCAGAAGGG 
GACCCGGCAC AGCTCCCCGT GCTGCGTCTA GAGGAAGCCC GGCCTCCTCT AGCTGGGGAC 
TGTGAACTGG ATTCGGAGAA CACATCTGCT GTCAGAAAGA GCCAGCTTCC AGCGGAAAAA 
GATGCTGGGA CCACGTGGGC TGCAGCCGTT CCTGAACAGG ACAGAGCACT TGCCATCGGG 
ACCCAGGGTG GGTCATCCAC GGCACCAGGG GAGCAAGACC TTCTCCCGGT CTCAAGGGAA 
GACCTAGCAG ATATGGTGGT GGACATAGGC ACTCCAGGGG AGCCCCAACC GCAGAGAGAG 
GGGGCCCAGA CCACCACAGG AAGGGAGAGA GAACCATATG GGAATAGGGC CACAGACTCT 
GGAGACAACC TCCACGATTC TGAAGATCTG GACCTACCAG CTACCCAGTG CTTTGTAGAC 
AGAAAGAATC AGAGCCTGGA AGGTTCCTTG GATGAGTCAT GGGAGGCCTT GGCGACACAG 
CCATTCTGTC CGAGAGAATC TGAGGCCTCC GAGACCCAAA CCGTTGTCAC CCTCCTTGAC 
ACCCATGCAT CTTGGCCCTC TCCATCTAGG ACAGCACAGC AAGAGCAACA TCCAGAGAGC 
CCAGTCCATG CAGAGCCACT GGGGATGGAA GGCAGAGAGA TGCAGACTGT GGAGAAAGAA 
ATGGGTACCC CAAGAGAAAC AGCAGAGAGG GTGATCCCTG AGGGAGGGCC ACCGCAGGAG 
GAAACCAAGA AACTGCCCTC AGAAGGAGAG AGGGAAGATG TGACGGGAGA GGAAGAATTA 
ATCCGGGGGA TACAGGGCAG TGAAAAAAAT CAGGTGTTTG CTAGAGATAC TCAGAGCCAA 
GAATCTGACA AAAAAGTGAA AAGTGCAAGT ACTGGAAGGG GAATGGAGAT TTTAAAGGTA 
GAAATTGAGA CACCCAAGGA AACACAAGAG AAAGAGAGAG AAAAGCAGAC TCTCGCAGGG 
GAAATATTTG AGAGTGAAGC AGGGAAACTG GTAGTAGAGA GAGAGAGTGA GGCAGATGGG 
TTAGAAGTCA AGGAACCCCA AGAGCTACTG GACAGAAGCC CACAGATGGG GGAGACAGAG 
GCGGGGGACC AGGACCAGAA AGGCCAAGCC TCTGGTCTGC CATCAGAGCC TGGAGCAGGG 
GCAGGAGACC TTCAGGGACT TGCTTCAGAC CCAGTAGCTT CTGGGAGCCA GGCAGGTGGA 
GGAAGGGGAG CCCCAGGGAG CCCCAGGAGG CAGCAGAGAG GTGACTTGAA TTGCGAGATG 
CCACCTGCTC AGAAGGCTTC CAGGGGTGAT CAGGAATCCC CAGATGCTTG TCTGCCTCCT 
GCAGCGCCTG AAGCCTCAGC CGCACTCCCA AATTCCCTCA TCATCTCTCA AATCCAAAAA 
CATCCCGCAC CTCAGTCCCT CCTTTTTCCC TCTCTAGCTC CTTTAGAACT GCCTATTCCC 
AGGACCAGAC AAAATGAGAG TCAGGAAGCT CCAGAGACTC CCTTCTCCTC AGAGCTGAAC 
TCTGTCCACC CAGAACCCAA AGTCAGGCTC CAGGCGTCCT CTCCAGTTTC TTCTCTACCC 
CTTGAGCCCC ACCCTACCAC CCCCACAGGC CAGCCTATTG CCCTTGAACC CATATCTGGG 
GTCTCTCGGA GCAGGACACG TAGTTCCTTT GATGTAACTG CCTCATCAGT TGTCCCCACA 
GCCGTTGCAC TGCAGCCATC CACCTCCACA GACAAGCCTG TCACCCCTAA GCCCACACTT 
CGGGCCCCTC GGGGCAGGGC ACAGAGGTCT TCTGTCAAAA CCCCTGAACC CAATGTCACC 
ACAGACCAGC CTGTTGCCCC TGAGCTCACA GCTATGGCAA CTCGGGGCAG GGCACAGAGG 
TCTTCTGTCA AGATTCCCAA ACCAGATAAC CCCACAACAC CCAAGCCCCA GCCTTCCACT 
TCCACAGACC AGCCTGTCAT CCCCAAACCC ACATCTCGGG CCCCTCGGGG CAGGACACCT 
AGGTCTTCTG CCAAGACTCC TGAACCAGCT GTCCCCACAG CCTCTGAGCT CCAGCCTGCT 
GCCCCTAAAG ACCAGCCTGT TGCTCCTGAG CTCACATCTA GAGCCACTCG GGGCAAGACA 
CAGAGGTCTA TCAAGACTTC CAAACCAGAT ATGCCCACAA CTCCCGAGCC CCAGCCTTCC 
ACCTCCACAG ACCAGCCTGT CACCCCCAAA CCCACGTCTC GGGCCCCTCG GGGCAGGACA 
CCTAGGTCTT CCGCCAAGAC TCCTGAGCCA GTTGTCTCCA CAGCCTCTGA GCTCCAGCCT 
TCTGCCCTCA CAGATGAGCC TGTCACTCCT GAGCTCACAT CTAGGGCTAC TCGGGGTCGG 
GCACAGAGGT CCTCTGTCAA AACCCCTGAT CCAGTTACCA CCACAACACC TGAGCTCCAG 
CCTTCCACCT CCACAGACCA GCTTGTCACC CCCAAACGCA CATCTCGGGC CCCTCGAGGC 
AGGACACGTA GGTTGTCTGC CAAGACTCCT GAACCAGTTG TTCCCACAGC CTCTGAGCTC 
CAGCCTTCTG CCCCTGCAGA CCAGCCTGTT GGTCCTTGGG CCACTCAGTG TAGAAGACAT 
AGGTCTTCTG TCAAGACCCC AGAACCAGTT GTCCCCACAG TCCCTGAACC TCAGCCTTCC 
ACTTCTAAAG ACCAGTCTGT CACTCCTGAG CCCACATCTC AGGCCACTGA GAGCCAAACA 
CATAGGTCCT CTATCAAGAC ATCCCAGCCA ACGGAACCCA CAGCCTCTGA CCTCAAACCT 
TCCTCCCCCA CAGACCAGCT TGTCACTCCC AAGGTCATAG CTCAGGGTGG TCCAAGCAGG 
ACAGGAAAGG CTTCCACAGC AAGTGCTGTG CTGGTTCCTA CTACCCCTGA ATTCCAGTCT 
CCAGTCCCGT CAGAACAGCC TCTTCCCCCT GACCCCATCC CTGAAGTCAA CTGCAGCAGG 
AGGCTGAGGG CCACTAGGAA ACATGGGTCT CCCACAGCTC ATGTTCATGA GCCCTGCACC 
GTACCCCCTG AACCTAACTC CCACTCTTCA AGGAACCAAA GACATGGGGC AGTGAAAGCA 
GCCAAGCCCC TTAGCACCAT TCCTGAGCCT GCCTTTGCCC AGCTCCCCGA GGCACCGCCT 
CACACTCCCC AGGTGCCAAA GGAGGAGGCA GCAGATGGAT CAGGCTTCAC CCCAGAGCCC 
CAGCCTAGGG CCTCTCAAAA CCACAAGAGG CCTTCAGCTA CTGCACATTC ACCTCCACTT 
CAAAAACGGC TCCAGAGAGG GAGAGTCCCT CAGAAGGCAG CATCCCTTAA GGAAGAAGAA 
AATCCAGTAG CGAGGCCGAG GAAGGAAGAG GGTGTAGTGA TTCCAGGTCC AGGCAAGAGA 
AAGAGAGAGC AGACAGAGGA GGAGTCCCAG GGAAGACCGA GCCGCAGCCT GCGACGGACC 
AAACCGATGC AGGAGTCCAC GGCCCCCAAA GTGCTGTTCA CCGGTGTGGT GGATGCTCGC 
GGAGAGAGGA CGGTGCTGGC CCTGGGGGGC AGTTTGGCCA GCTCGGTGGC CGAGGCTTCT 
CACCTGGTGA CTGATCGGAT CCGCCGGACG GTCAAGTTTC TGTGTGCCCT GGGCCGGGGC 
ATCCCCATCC TCTCCCTGGC CTGGCTGCAT GAGTCCCGCA AGGCAGGCTG CTTCTTGCCC 
CCGGACGAAT ATTTGGTGAC TGATCCTGAG CAGGAGAAGA ACTTCGGCTT CAGCCTTCGG 
GAGGCCCTGA GCCGAGCTCA GGAGCGAAGG CTGCTGGAGG GCTATGAGAT TCACGTGACC 
CCCGGAGTCC AGCCACCGCC ACCTCAGATG GGAGAAATCA TCAGCTGCTG TGGAGGCGCC 
ATCCTGCCCA GCATGCCCCG GTCCTACAAG CCTCAGAGGT TCGTGATCAC ATGTTCCCAG 
GACTTTCCTC GATGTGCTGT TCCATATCGG GTTGGGCTGC CTATCCTCTC ACCCGAATTC 
CTGCTGACGG GAGTACTGAA GCAGGAAGTC AAGCCAGAGG CCTTTGCCTT CTCCACTGTG 
GAAATGTCAT CCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MDC1 ( XM_012100988.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_011956378.1 Ovis aries mediator of DNA damage checkpoint 1 (MDC1), transcript variant X5, mRNA.

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CloneID	OSm28029
Clone ID Related Accession (Same CDS sequence)	XM_012100988.2
Accession Version	XM_012100988.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5235bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries(sheep)
Product	mediator of DNA damage checkpoint protein 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014639029.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012100988.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGTGT CCCACTTTCC TTGTCTACAT TTTCCCTTTA AGATCATGGA AGACACCCAG 
ATTTTAAACT GGGAAGTTGA AGAAGAGGAG GAGGTTGCAG AGAGCCCCAA TGAATCTCTG 
GGGTATAGCT TGGAGCCCCT AGCACAGCTG CATATCTTCA GTAGTTCCTA TGGACCAGAA 
AAAGACTTTC CACTCTACCT TGGAAAGAAC ATGATAGGCC GAATGCCTGA TTGCTCTGTG 
GCCCTGCCCT TTTCATCAAT ATCCAAACAA CATGCAGTGA TTGAAATCTC TGCCTGGGAC 
AAGGCGCCTG TCCTTCGGGA TTGCGGGAGC CTCAATGGCA CTCAAATCCT GCGGCCTCCT 
AAGGTCCTGA GCCCTGGGGT GAGTCATCGT TTGCGGGACC GGGAGTTGAT TCTCTTTGCT 
GACTTGCCCT GCCAGTACCA TCGCTTGGAT GTCCCCCGGC CTTTTGTCTC CCGGGGCCCT 
CTAAGTGTAG AGGAGACACC CAGGGTACAG GGAGGAACTC AACCCTCCAG GCTTCTGTTG 
GCTGAGGACT CAGAGGAAGA AGTAGATTCC CTTTTGGACA AGTGTGTGGT GAAAGGACCA 
AGGACCTCCT CTTCGGCAAC AGTCGTTCCA GAGAGTGATG AAGAAGGGCC TTCCCCTGCC 
CCAGATGGGC CCGGGCCACC TTCTGCCTTC AACTTGAACA GCGACACGGA TGAGGAAGAA 
AGTCAGGAAT CAGGAGCAGG GGAGGCCTCT TCAGCTGCCA GAAGAGGTAC CGCTGCAGAG 
ACGGAACAGC CTGAACCTGT CACAGCCGAG ATCCAGATTG AGAAGGATCA GTGTTCCGTG 
AAGGAGAGGA ACGGGGACAC AGAAATCGAG AGGGATGTGA GGAATGGGGT GGTTCCGACT 
GGAGTGATTC TGGAGAGGAG CCAGCCTTCT GGGGAGGACA GTGACACAGA TGTGAGTGAT 
GAGAGTGGGC CTCGAAGAAG GCTGGTTGGA GTCCGTCCGA AAAGGGCCTG GTCTTGTAAC 
TTCATAGACA GTGATACCGA TGGAGAGGAC GAGGGGATCC CTGCTACCCC AGCAGTGGTT 
CCCATGAAGG AGAGGCAGAT CTTCCACGAA GCTGGTACAC AGAGCCCCCA GGCACCTGGT 
GTGGCACGTT GGCAGGAGAG CCCAGCTGAT GGTGATACAG ATATAGAGGA GGGGGAGGCC 
CCCCCAGACA GAAGCCAAGC CTCCGTGGTG ATCGACAGCA ATACAGACGA TGAGGAAGAA 
GTCTCAGCAG CACTGACACT GGCATGTCTA AGAGAGAGCC AGGCTGGTAA GTGGAACCGA 
GATCCAGATG CAGAAGAGAC CAGGGCCCAA CCCGTGGCCC TTCTGGAGCG AAGCCAGGCC 
TCTGCTGGGG GAGACAGTGA CACAGATGTG GAGGAAGAGG GGCTCCCAGT GGAAAGGAGG 
GGAATGGTTC CCAAGGGTTA CATGGACAGG GAATATTCAA AAAAGAGCCA GCACCCTCCC 
AGGGACAGTG ATACAGAGGG GAAGGAAGAT GAGAGCTCAC CGGGGGTCCA CCTGGAGAGA 
AGCCAGGCCT CTGCACAAGT GGAGGATGAG GTCCCACTGG GGCCAGCTGT TGCACTTCCG 
GAGAAGCGTC AGGTACAAGG CATAGTGTGG CCACATCACA CCGATGCGGA GGCAGAAGGG 
GACCCGGCAC AGCTCCCCGT GCTGCGTCTA GAGGAAGCCC GGCCTCCTCT AGCTGGGGAC 
TGTGAACTGG ATTCGGAGAA CACATCTGCT GTCAGAAAGA GCCAGCTTCC AGCGGAAAAA 
GATGCTGGGA CCACGTGGGC TGCAGCCGTT CCTGAACAGG ACAGAGCACT TGCCATCGGG 
ACCCAGGGTG GGTCATCCAC GGCACCAGGG GAGCAAGACC TTCTCCCGGT CTCAAGGGAA 
GACCTAGCAG ATATGGTGGT GGACATAGGC ACTCCAGGGG AGCCCCAACC GCAGAGAGAG 
GGGGCCCAGA CCACCACAGG AAGGGAGAGA GAACCATATG GGAATAGGGC CACAGACTCT 
GGAGACAACC TCCACGATTC TGAAGATCTG GACCTACCAG CTACCCAGTG CTTTGTAGAC 
AGAAAGAATC AGAGCCTGGA AGCAGCCCCC AGCATGGAGG ATGAGCCCAC CCAGGCCTTC 
CTGTTTCCTC TGCCCCAAGA GCCTGGCCCT TCCTGTTGCA GCTCCCAGGC CACAGGTTCC 
TTGGATGAGT CATGGGAGGC CTTGGCGACA CAGCCATTCT GTCCGAGAGA ATCTGAGGCC 
TCCGAGACCC AAACCGTTGT CACCCTCCTT GACACCCATG CATCTTGGCC CTCTCCATCT 
AGGACAGCAC AGCAAGAGCA ACATCCAGAG AGCCCAGTCC ATGCAGAGCC ACTGGGGATG 
GAAGGCAGAG AGATGCAGAC TGTGGAGAAA GAAATGGGTG ACTTGAATTG CGAGATGCCA 
CCTGCTCAGA AGGCTTCCAG GGGTGATCAG GAATCCCCAG ATGCTTGTCT GCCTCCTGCA 
GCGCCTGAAG CCTCAGCCGC ACTCCCAAAT TCCCTCATCA TCTCTCAAAT CCAAAAACAT 
CCCGCACCTC AGTCCCTCCT TTTTCCCTCT CTAGCTCCTT TAGAACTGCC TATTCCCAGG 
ACCAGACAAA ATGAGAGTCA GGAAGCTCCA GAGACTCCCT TCTCCTCAGA GCTGAACTCT 
GTCCACCCAG AACCCAAAGT CAGGCTCCAG GCGTCCTCTC CAGTTTCTTC TCTACCCCTT 
GAGCCCCACC CTACCACCCC CACAGGCCAG CCTATTGCCC TTGAACCCAT ATCTGGGGTC 
TCTCGGAGCA GGACACGTAG TTCCTTTGAT GTAACTGCCT CATCAGTTGT CCCCACAGCC 
GTTGCACTGC AGCCATCCAC CTCCACAGAC AAGCCTGTCA CCCCTAAGCC CACACTTCGG 
GCCCCTCGGG GCAGGGCACA GAGGTCTTCT GTCAAAACCC CTGAACCCAA TGTCACCACA 
GACCAGCCTG TTGCCCCTGA GCTCACAGCT ATGGCAACTC GGGGCAGGGC ACAGAGGTCT 
TCTGTCAAGA TTCCCAAACC AGATAACCCC ACAACACCCA AGCCCCAGCC TTCCACTTCC 
ACAGACCAGC CTGTCATCCC CAAACCCACA TCTCGGGCCC CTCGGGGCAG GACACCTAGG 
TCTTCTGCCA AGACTCCTGA ACCAGCTGTC CCCACAGCCT CTGAGCTCCA GCCTGCTGCC 
CCTAAAGACC AGCCTGTTGC TCCTGAGCTC ACATCTAGAG CCACTCGGGG CAAGACACAG 
AGGTCTATCA AGACTTCCAA ACCAGATATG CCCACAACTC CCGAGCCCCA GCCTTCCACC 
TCCACAGACC AGCCTGTCAC CCCCAAACCC ACGTCTCGGG CCCCTCGGGG CAGGACACCT 
AGGTCTTCCG CCAAGACTCC TGAGCCAGTT GTCTCCACAG CCTCTGAGCT CCAGCCTTCT 
GCCCTCACAG ATGAGCCTGT CACTCCTGAG CTCACATCTA GGGCTACTCG GGGTCGGGCA 
CAGAGGTCCT CTGTCAAAAC CCCTGATCCA GTTACCACCA CAACACCTGA GCTCCAGCCT 
TCCACCTCCA CAGACCAGCT TGTCACCCCC AAACGCACAT CTCGGGCCCC TCGAGGCAGG 
ACACGTAGGT TGTCTGCCAA GACTCCTGAA CCAGTTGTTC CCACAGCCTC TGAGCTCCAG 
CCTTCTGCCC CTGCAGACCA GCCTGTTGGT CCTTGGGCCA CTCAGTGTAG AAGACATAGG 
TCTTCTGTCA AGACCCCAGA ACCAGTTGTC CCCACAGTCC CTGAACCTCA GCCTTCCACT 
TCTAAAGACC AGTCTGTCAC TCCTGAGCCC ACATCTCAGG CCACTGAGAG CCAAACACAT 
AGGTCCTCTA TCAAGACATC CCAGCCAACG GAACCCACAG CCTCTGACCT CAAACCTTCC 
TCCCCCACAG ACCAGCTTGT CACTCCCAAG GTCATAGCTC AGGGTGGTCC AAGCAGGACA 
GGAAAGGCTT CCACAGCAAG TGCTGTGCTG GTTCCTACTA CCCCTGAATT CCAGTCTCCA 
GTCCCGTCAG AACAGCCTCT TCCCCCTGAC CCCATCCCTG AAGTCAACTG CAGCAGGAGG 
CTGAGGGCCA CTAGGAAACA TGGGTCTCCC ACAGCTCATG TTCATGAGCC CTGCACCGTA 
CCCCCTGAAC CTAACTCCCA CTCTTCAAGG AACCAAAGAC ATGGGGCAGT GAAAGCAGCC 
AAGCCCCTTA GCACCATTCC TGAGCCTGCC TTTGCCCAGC TCCCCGAGGC ACCGCCTCAC 
ACTCCCCAGG TGCCAAAGGA GGAGGCAGCA GATGGATCAG GCTTCACCCC AGAGCCCCAG 
CCTAGGGCCT CTCAAAACCA CAAGAGGCCT TCAGCTACTG CACATTCACC TCCACTTCAA 
AAACGGCTCC AGAGAGGGAG AGTCCCTCAG AAGGCAGCAT CCCTTAAGGA AGAAGAAAAT 
CCAGTAGCGA GGCCGAGGAA GGAAGAGGGT GTAGTGATTC CAGGTCCAGG CAAGAGAAAG 
AGAGAGCAGA CAGAGGAGGA GTCCCAGGGA AGACCGAGCC GCAGCCTGCG ACGGACCAAA 
CCGATGCAGG AGTCCACGGC CCCCAAAGTG CTGTTCACCG GTGTGGTGGA TGCTCGCGGA 
GAGAGGACGG TGCTGGCCCT GGGGGGCAGT TTGGCCAGCT CGGTGGCCGA GGCTTCTCAC 
CTGGTGACTG ATCGGATCCG CCGGACGGTC AAGTTTCTGT GTGCCCTGGG CCGGGGCATC 
CCCATCCTCT CCCTGGCCTG GCTGCATGAG TCCCGCAAGG CAGGCTGCTT CTTGCCCCCG 
GACGAATATT TGGTGACTGA TCCTGAGCAG GAGAAGAACT TCGGCTTCAG CCTTCGGGAG 
GCCCTGAGCC GAGCTCAGGA GCGAAGGCTG CTGGAGGGCT ATGAGATTCA CGTGACCCCC 
GGAGTCCAGC CACCGCCACC TCAGATGGGA GAAATCATCA GCTGCTGTGG AGGCGCCATC 
CTGCCCAGCA TGCCCCGGTC CTACAAGCCT CAGAGGTTCG TGATCACATG TTCCCAGGAC 
TTTCCTCGAT GTGCTGTTCC ATATCGGGTT GGGCTGCCTA TCCTCTCACC CGAATTCCTG 
CTGACGGGAG TACTGAAGCA GGAAGTCAAG CCAGAGGCCT TTGCCTTCTC CACTGTGGAA 
ATGTCATCCA CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011956378.1
CDS	144..5378
Translation	MEVSHFPCLHFPFKIMEDTQILNWEVEEEEEVAESPNESLGYSLEPLAQLHIFSSSYGPEKDFPLYLGKNMIGRMPDCSVALPFSSISKQHAVIEISAWDKAPVLRDCGSLNGTQILRPPKVLSPGVSHRLRDRELILFADLPCQYHRLDVPRPFVSRGPLSVEETPRVQGGTQPSRLLLAEDSEEEVDSLLDKCVVKGPRTSSSATVVPESDEEGPSPAPDGPGPPSAFNLNSDTDEEESQESGAGEASSAARRGTAAETEQPEPVTAEIQIEKDQCSVKERNGDTEIERDVRNGVVPTGVILERSQPSGEDSDTDVSDESGPRRRLVGVRPKRAWSCNFIDSDTDGEDEGIPATPAVVPMKERQIFHEAGTQSPQAPGVARWQESPADGDTDIEEGEAPPDRSQASVVIDSNTDDEEEVSAALTLACLRESQAGKWNRDPDAEETRAQPVALLERSQASAGGDSDTDVEEEGLPVERRGMVPKGYMDREYSKKSQHPPRDSDTEGKEDESSPGVHLERSQASAQVEDEVPLGPAVALPEKRQVQGIVWPHHTDAEAEGDPAQLPVLRLEEARPPLAGDCELDSENTSAVRKSQLPAEKDAGTTWAAAVPEQDRALAIGTQGGSSTAPGEQDLLPVSREDLADMVVDIGTPGEPQPQREGAQTTTGREREPYGNRATDSGDNLHDSEDLDLPATQCFVDRKNQSLEAAPSMEDEPTQAFLFPLPQEPGPSCCSSQATGSLDESWEALATQPFCPRESEASETQTVVTLLDTHASWPSPSRTAQQEQHPESPVHAEPLGMEGREMQTVEKEMGDLNCEMPPAQKASRGDQESPDACLPPAAPEASAALPNSLIISQIQKHPAPQSLLFPSLAPLELPIPRTRQNESQEAPETPFSSELNSVHPEPKVRLQASSPVSSLPLEPHPTTPTGQPIALEPISGVSRSRTRSSFDVTASSVVPTAVALQPSTSTDKPVTPKPTLRAPRGRAQRSSVKTPEPNVTTDQPVAPELTAMATRGRAQRSSVKIPKPDNPTTPKPQPSTSTDQPVIPKPTSRAPRGRTPRSSAKTPEPAVPTASELQPAAPKDQPVAPELTSRATRGKTQRSIKTSKPDMPTTPEPQPSTSTDQPVTPKPTSRAPRGRTPRSSAKTPEPVVSTASELQPSALTDEPVTPELTSRATRGRAQRSSVKTPDPVTTTTPELQPSTSTDQLVTPKRTSRAPRGRTRRLSAKTPEPVVPTASELQPSAPADQPVGPWATQCRRHRSSVKTPEPVVPTVPEPQPSTSKDQSVTPEPTSQATESQTHRSSIKTSQPTEPTASDLKPSSPTDQLVTPKVIAQGGPSRTGKASTASAVLVPTTPEFQSPVPSEQPLPPDPIPEVNCSRRLRATRKHGSPTAHVHEPCTVPPEPNSHSSRNQRHGAVKAAKPLSTIPEPAFAQLPEAPPHTPQVPKEEAADGSGFTPEPQPRASQNHKRPSATAHSPPLQKRLQRGRVPQKAASLKEEENPVARPRKEEGVVIPGPGKRKREQTEEESQGRPSRSLRRTKPMQESTAPKVLFTGVVDARGERTVLALGGSLASSVAEASHLVTDRIRRTVKFLCALGRGIPILSLAWLHESRKAGCFLPPDEYLVTDPEQEKNFGFSLREALSRAQERRLLEGYEIHVTPGVQPPPPQMGEIISCCGGAILPSMPRSYKPQRFVITCSQDFPRCAVPYRVGLPILSPEFLLTGVLKQEVKPEAFAFSTVEMSST

Target ORF information:

RefSeq Version	XM_012100988.2
Organism	Ovis aries(sheep)
Definition	Ovis aries mediator of DNA damage checkpoint 1 (MDC1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012100988.2

ORF Insert Sequence:

ATGGAGGTGT CCCACTTTCC TTGTCTACAT TTTCCCTTTA AGATCATGGA AGACACCCAG 
ATTTTAAACT GGGAAGTTGA AGAAGAGGAG GAGGTTGCAG AGAGCCCCAA TGAATCTCTG 
GGGTATAGCT TGGAGCCCCT AGCACAGCTG CATATCTTCA GTAGTTCCTA TGGACCAGAA 
AAAGACTTTC CACTCTACCT TGGAAAGAAC ATGATAGGCC GAATGCCTGA TTGCTCTGTG 
GCCCTGCCCT TTTCATCAAT ATCCAAACAA CATGCAGTGA TTGAAATCTC TGCCTGGGAC 
AAGGCGCCTG TCCTTCGGGA TTGCGGGAGC CTCAATGGCA CTCAAATCCT GCGGCCTCCT 
AAGGTCCTGA GCCCTGGGGT GAGTCATCGT TTGCGGGACC GGGAGTTGAT TCTCTTTGCT 
GACTTGCCCT GCCAGTACCA TCGCTTGGAT GTCCCCCGGC CTTTTGTCTC CCGGGGCCCT 
CTAAGTGTAG AGGAGACACC CAGGGTACAG GGAGGAACTC AACCCTCCAG GCTTCTGTTG 
GCTGAGGACT CAGAGGAAGA AGTAGATTCC CTTTTGGACA AGTGTGTGGT GAAAGGACCA 
AGGACCTCCT CTTCGGCAAC AGTCGTTCCA GAGAGTGATG AAGAAGGGCC TTCCCCTGCC 
CCAGATGGGC CCGGGCCACC TTCTGCCTTC AACTTGAACA GCGACACGGA TGAGGAAGAA 
AGTCAGGAAT CAGGAGCAGG GGAGGCCTCT TCAGCTGCCA GAAGAGGTAC CGCTGCAGAG 
ACGGAACAGC CTGAACCTGT CACAGCCGAG ATCCAGATTG AGAAGGATCA GTGTTCCGTG 
AAGGAGAGGA ACGGGGACAC AGAAATCGAG AGGGATGTGA GGAATGGGGT GGTTCCGACT 
GGAGTGATTC TGGAGAGGAG CCAGCCTTCT GGGGAGGACA GTGACACAGA TGTGAGTGAT 
GAGAGTGGGC CTCGAAGAAG GCTGGTTGGA GTCCGTCCGA AAAGGGCCTG GTCTTGTAAC 
TTCATAGACA GTGATACCGA TGGAGAGGAC GAGGGGATCC CTGCTACCCC AGCAGTGGTT 
CCCATGAAGG AGAGGCAGAT CTTCCACGAA GCTGGTACAC AGAGCCCCCA GGCACCTGGT 
GTGGCACGTT GGCAGGAGAG CCCAGCTGAT GGTGATACAG ATATAGAGGA GGGGGAGGCC 
CCCCCAGACA GAAGCCAAGC CTCCGTGGTG ATCGACAGCA ATACAGACGA TGAGGAAGAA 
GTCTCAGCAG CACTGACACT GGCATGTCTA AGAGAGAGCC AGGCTGGTAA GTGGAACCGA 
GATCCAGATG CAGAAGAGAC CAGGGCCCAA CCCGTGGCCC TTCTGGAGCG AAGCCAGGCC 
TCTGCTGGGG GAGACAGTGA CACAGATGTG GAGGAAGAGG GGCTCCCAGT GGAAAGGAGG 
GGAATGGTTC CCAAGGGTTA CATGGACAGG GAATATTCAA AAAAGAGCCA GCACCCTCCC 
AGGGACAGTG ATACAGAGGG GAAGGAAGAT GAGAGCTCAC CGGGGGTCCA CCTGGAGAGA 
AGCCAGGCCT CTGCACAAGT GGAGGATGAG GTCCCACTGG GGCCAGCTGT TGCACTTCCG 
GAGAAGCGTC AGGTACAAGG CATAGTGTGG CCACATCACA CCGATGCGGA GGCAGAAGGG 
GACCCGGCAC AGCTCCCCGT GCTGCGTCTA GAGGAAGCCC GGCCTCCTCT AGCTGGGGAC 
TGTGAACTGG ATTCGGAGAA CACATCTGCT GTCAGAAAGA GCCAGCTTCC AGCGGAAAAA 
GATGCTGGGA CCACGTGGGC TGCAGCCGTT CCTGAACAGG ACAGAGCACT TGCCATCGGG 
ACCCAGGGTG GGTCATCCAC GGCACCAGGG GAGCAAGACC TTCTCCCGGT CTCAAGGGAA 
GACCTAGCAG ATATGGTGGT GGACATAGGC ACTCCAGGGG AGCCCCAACC GCAGAGAGAG 
GGGGCCCAGA CCACCACAGG AAGGGAGAGA GAACCATATG GGAATAGGGC CACAGACTCT 
GGAGACAACC TCCACGATTC TGAAGATCTG GACCTACCAG CTACCCAGTG CTTTGTAGAC 
AGAAAGAATC AGAGCCTGGA AGCAGCCCCC AGCATGGAGG ATGAGCCCAC CCAGGCCTTC 
CTGTTTCCTC TGCCCCAAGA GCCTGGCCCT TCCTGTTGCA GCTCCCAGGC CACAGGTTCC 
TTGGATGAGT CATGGGAGGC CTTGGCGACA CAGCCATTCT GTCCGAGAGA ATCTGAGGCC 
TCCGAGACCC AAACCGTTGT CACCCTCCTT GACACCCATG CATCTTGGCC CTCTCCATCT 
AGGACAGCAC AGCAAGAGCA ACATCCAGAG AGCCCAGTCC ATGCAGAGCC ACTGGGGATG 
GAAGGCAGAG AGATGCAGAC TGTGGAGAAA GAAATGGGTG ACTTGAATTG CGAGATGCCA 
CCTGCTCAGA AGGCTTCCAG GGGTGATCAG GAATCCCCAG ATGCTTGTCT GCCTCCTGCA 
GCGCCTGAAG CCTCAGCCGC ACTCCCAAAT TCCCTCATCA TCTCTCAAAT CCAAAAACAT 
CCCGCACCTC AGTCCCTCCT TTTTCCCTCT CTAGCTCCTT TAGAACTGCC TATTCCCAGG 
ACCAGACAAA ATGAGAGTCA GGAAGCTCCA GAGACTCCCT TCTCCTCAGA GCTGAACTCT 
GTCCACCCAG AACCCAAAGT CAGGCTCCAG GCGTCCTCTC CAGTTTCTTC TCTACCCCTT 
GAGCCCCACC CTACCACCCC CACAGGCCAG CCTATTGCCC TTGAACCCAT ATCTGGGGTC 
TCTCGGAGCA GGACACGTAG TTCCTTTGAT GTAACTGCCT CATCAGTTGT CCCCACAGCC 
GTTGCACTGC AGCCATCCAC CTCCACAGAC AAGCCTGTCA CCCCTAAGCC CACACTTCGG 
GCCCCTCGGG GCAGGGCACA GAGGTCTTCT GTCAAAACCC CTGAACCCAA TGTCACCACA 
GACCAGCCTG TTGCCCCTGA GCTCACAGCT ATGGCAACTC GGGGCAGGGC ACAGAGGTCT 
TCTGTCAAGA TTCCCAAACC AGATAACCCC ACAACACCCA AGCCCCAGCC TTCCACTTCC 
ACAGACCAGC CTGTCATCCC CAAACCCACA TCTCGGGCCC CTCGGGGCAG GACACCTAGG 
TCTTCTGCCA AGACTCCTGA ACCAGCTGTC CCCACAGCCT CTGAGCTCCA GCCTGCTGCC 
CCTAAAGACC AGCCTGTTGC TCCTGAGCTC ACATCTAGAG CCACTCGGGG CAAGACACAG 
AGGTCTATCA AGACTTCCAA ACCAGATATG CCCACAACTC CCGAGCCCCA GCCTTCCACC 
TCCACAGACC AGCCTGTCAC CCCCAAACCC ACGTCTCGGG CCCCTCGGGG CAGGACACCT 
AGGTCTTCCG CCAAGACTCC TGAGCCAGTT GTCTCCACAG CCTCTGAGCT CCAGCCTTCT 
GCCCTCACAG ATGAGCCTGT CACTCCTGAG CTCACATCTA GGGCTACTCG GGGTCGGGCA 
CAGAGGTCCT CTGTCAAAAC CCCTGATCCA GTTACCACCA CAACACCTGA GCTCCAGCCT 
TCCACCTCCA CAGACCAGCT TGTCACCCCC AAACGCACAT CTCGGGCCCC TCGAGGCAGG 
ACACGTAGGT TGTCTGCCAA GACTCCTGAA CCAGTTGTTC CCACAGCCTC TGAGCTCCAG 
CCTTCTGCCC CTGCAGACCA GCCTGTTGGT CCTTGGGCCA CTCAGTGTAG AAGACATAGG 
TCTTCTGTCA AGACCCCAGA ACCAGTTGTC CCCACAGTCC CTGAACCTCA GCCTTCCACT 
TCTAAAGACC AGTCTGTCAC TCCTGAGCCC ACATCTCAGG CCACTGAGAG CCAAACACAT 
AGGTCCTCTA TCAAGACATC CCAGCCAACG GAACCCACAG CCTCTGACCT CAAACCTTCC 
TCCCCCACAG ACCAGCTTGT CACTCCCAAG GTCATAGCTC AGGGTGGTCC AAGCAGGACA 
GGAAAGGCTT CCACAGCAAG TGCTGTGCTG GTTCCTACTA CCCCTGAATT CCAGTCTCCA 
GTCCCGTCAG AACAGCCTCT TCCCCCTGAC CCCATCCCTG AAGTCAACTG CAGCAGGAGG 
CTGAGGGCCA CTAGGAAACA TGGGTCTCCC ACAGCTCATG TTCATGAGCC CTGCACCGTA 
CCCCCTGAAC CTAACTCCCA CTCTTCAAGG AACCAAAGAC ATGGGGCAGT GAAAGCAGCC 
AAGCCCCTTA GCACCATTCC TGAGCCTGCC TTTGCCCAGC TCCCCGAGGC ACCGCCTCAC 
ACTCCCCAGG TGCCAAAGGA GGAGGCAGCA GATGGATCAG GCTTCACCCC AGAGCCCCAG 
CCTAGGGCCT CTCAAAACCA CAAGAGGCCT TCAGCTACTG CACATTCACC TCCACTTCAA 
AAACGGCTCC AGAGAGGGAG AGTCCCTCAG AAGGCAGCAT CCCTTAAGGA AGAAGAAAAT 
CCAGTAGCGA GGCCGAGGAA GGAAGAGGGT GTAGTGATTC CAGGTCCAGG CAAGAGAAAG 
AGAGAGCAGA CAGAGGAGGA GTCCCAGGGA AGACCGAGCC GCAGCCTGCG ACGGACCAAA 
CCGATGCAGG AGTCCACGGC CCCCAAAGTG CTGTTCACCG GTGTGGTGGA TGCTCGCGGA 
GAGAGGACGG TGCTGGCCCT GGGGGGCAGT TTGGCCAGCT CGGTGGCCGA GGCTTCTCAC 
CTGGTGACTG ATCGGATCCG CCGGACGGTC AAGTTTCTGT GTGCCCTGGG CCGGGGCATC 
CCCATCCTCT CCCTGGCCTG GCTGCATGAG TCCCGCAAGG CAGGCTGCTT CTTGCCCCCG 
GACGAATATT TGGTGACTGA TCCTGAGCAG GAGAAGAACT TCGGCTTCAG CCTTCGGGAG 
GCCCTGAGCC GAGCTCAGGA GCGAAGGCTG CTGGAGGGCT ATGAGATTCA CGTGACCCCC 
GGAGTCCAGC CACCGCCACC TCAGATGGGA GAAATCATCA GCTGCTGTGG AGGCGCCATC 
CTGCCCAGCA TGCCCCGGTC CTACAAGCCT CAGAGGTTCG TGATCACATG TTCCCAGGAC 
TTTCCTCGAT GTGCTGTTCC ATATCGGGTT GGGCTGCCTA TCCTCTCACC CGAATTCCTG 
CTGACGGGAG TACTGAAGCA GGAAGTCAAG CCAGAGGCCT TTGCCTTCTC CACTGTGGAA 
ATGTCATCCA CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MDC1 ( XM_012100990.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_011956380.1 Ovis aries mediator of DNA damage checkpoint 1 (MDC1), transcript variant X6, mRNA.

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CloneID	OSm28030
Clone ID Related Accession (Same CDS sequence)	XM_012100990.2
Accession Version	XM_012100990.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5061bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries(sheep)
Product	mediator of DNA damage checkpoint protein 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014639029.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012100990.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGTGT CCCACTTTCC TTGTCTACAT TTTCCCTTTA AGATCATGGA AGACACCCAG 
ATTTTAAACT GGGAAGTTGA AGAAGAGGAG GAGGTTGCAG AGAGCCCCAA TGAATCTCTG 
GGGTATAGCT TGGAGCCCCT AGCACAGCTG CATATCTTCA GTAGTTCCTA TGGACCAGAA 
AAAGACTTTC CACTCTACCT TGGAAAGAAC ATGATAGGCC GAATGCCTGA TTGCTCTGTG 
GCCCTGCCCT TTTCATCAAT ATCCAAACAA CATGCAGTGA TTGAAATCTC TGCCTGGGAC 
AAGGCGCCTG TCCTTCGGGA TTGCGGGAGC CTCAATGGCA CTCAAATCCT GCGGCCTCCT 
AAGGTCCTGA GCCCTGGGGT GAGTCATCGT TTGCGGGACC GGGAGTTGAT TCTCTTTGCT 
GACTTGCCCT GCCAGTACCA TCGCTTGGAT GTCCCCCGGC CTTTTGTCTC CCGGGGCCCT 
CTAAGTGTAG AGGAGACACC CAGGGTACAG GGAGGAACTC AACCCTCCAG GCTTCTGTTG 
GCTGAGGACT CAGAGGAAGA AGTAGATTCC CTTTTGGACA AGTGTGTGGT GAAAGGACCA 
AGGACCTCCT CTTCGGCAAC AGTCGTTCCA GAGAGTGATG AAGAAGGGCC TTCCCCTGCC 
CCAGATGGGC CCGGGCCACC TTCTGCCTTC AACTTGAACA GCGACACGGA TGAGGAAGAA 
AGTCAGGAAT CAGGAGCAGG GGAGGCCTCT TCAGCTGCCA GAAGAGGTAC CGCTGCAGAG 
ACGGAACAGC CTGAACCTGT CACAGCCGAG ATCCAGATTG AGAAGGATCA GTGTTCCGTG 
AAGGAGAGGA ACGGGGACAC AGAAATCGAG AGGGATGTGA GGAATGGGGT GGTTCCGACT 
GGAGTGATTC TGGAGAGGAG CCAGCCTTCT GGGGAGGACA GTGACACAGA TGTGAGTGAT 
GAGAGTGGGC CTCGAAGAAG GCTGGTTGGA GTCCGTCCGA AAAGGGCCTG GTCTTGTAAC 
TTCATAGACA GTGATACCGA TGGAGAGGAC GAGGGGATCC CTGCTACCCC AGCAGTGGTT 
CCCATGAAGG AGAGGCAGAT CTTCCACGAA GCTGGTACAC AGAGCCCCCA GGCACCTGGT 
GTGGCACGTT GGCAGGAGAG CCCAGCTGAT GGTGATACAG ATATAGAGGA GGGGGAGGCC 
CCCCCAGACA GAAGCCAAGC CTCCGTGGTG ATCGACAGCA ATACAGACGA TGAGGAAGAA 
GTCTCAGCAG CACTGACACT GGCATGTCTA AGAGAGAGCC AGGCTGATTC TGAAGATCTG 
GACCTACCAG CTACCCAGTG CTTTGTAGAC AGAAAGAATC AGAGCCTGGA AGCAGCCCCC 
AGCATGGAGG ATGAGCCCAC CCAGGCCTTC CTGTTTCCTC TGCCCCAAGA GCCTGGCCCT 
TCCTGTTGCA GCTCCCAGGC CACAGGTTCC TTGGATGAGT CATGGGAGGC CTTGGCGACA 
CAGCCATTCT GTCCGAGAGA ATCTGAGGCC TCCGAGACCC AAACCGTTGT CACCCTCCTT 
GACACCCATG CATCTTGGCC CTCTCCATCT AGGACAGCAC AGCAAGAGCA ACATCCAGAG 
AGCCCAGTCC ATGCAGAGCC ACTGGGGATG GAAGGCAGAG AGATGCAGAC TGTGGAGAAA 
GAAATGGGTA CCCCAAGAGA AACAGCAGAG AGGGTGATCC CTGAGGGAGG GCCACCGCAG 
GAGGAAACCA AGAAACTGCC CTCAGAAGGA GAGAGGGAAG ATGTGACGGG AGAGGAAGAA 
TTAATCCGGG GGATACAGGG CAGTGAAAAA AATCAGGTGT TTGCTAGAGA TACTCAGAGC 
CAAGAATCTG ACAAAAAAGT GAAAAGTGCA AGTACTGGAA GGGGAATGGA GATTTTAAAG 
GTAGAAATTG AGACACCCAA GGAAACACAA GAGAAAGAGA GAGAAAAGCA GACTCTCGCA 
GGGGAAATAT TTGAGAGTGA AGCAGGGAAA CTGGTAGTAG AGAGAGAGAG TGAGGCAGAT 
GGGTTAGAAG TCAAGGAACC CCAAGAGCTA CTGGACAGAA GCCCACAGAT GGGGGAGACA 
GAGGCGGGGG ACCAGGACCA GAAAGGCCAA GCCTCTGGTC TGCCATCAGA GCCTGGAGCA 
GGGGCAGGAG ACCTTCAGGG ACTTGCTTCA GACCCAGTAG CTTCTGGGAG CCAGGCAGGT 
GGAGGAAGGG GAGCCCCAGG GAGCCCCAGG AGGCAGCAGA GAGGTGACTT GAATTGCGAG 
ATGCCACCTG CTCAGAAGGC TTCCAGGGGT GATCAGGAAT CCCCAGATGC TTGTCTGCCT 
CCTGCAGCGC CTGAAGCCTC AGCCGCACTC CCAAATTCCC TCATCATCTC TCAAATCCAA 
AAACATCCCG CACCTCAGTC CCTCCTTTTT CCCTCTCTAG CTCCTTTAGA ACTGCCTATT 
CCCAGGACCA GACAAAATGA GAGTCAGGAA GCTCCAGAGA CTCCCTTCTC CTCAGAGCTG 
AACTCTGTCC ACCCAGAACC CAAAGTCAGG CTCCAGGCGT CCTCTCCAGT TTCTTCTCTA 
CCCCTTGAGC CCCACCCTAC CACCCCCACA GGCCAGCCTA TTGCCCTTGA ACCCATATCT 
GGGGTCTCTC GGAGCAGGAC ACGTAGTTCC TTTGATGTAA CTGCCTCATC AGTTGTCCCC 
ACAGCCGTTG CACTGCAGCC ATCCACCTCC ACAGACAAGC CTGTCACCCC TAAGCCCACA 
CTTCGGGCCC CTCGGGGCAG GGCACAGAGG TCTTCTGTCA AAACCCCTGA ACCCAATGTC 
ACCACAGACC AGCCTGTTGC CCCTGAGCTC ACAGCTATGG CAACTCGGGG CAGGGCACAG 
AGGTCTTCTG TCAAGATTCC CAAACCAGAT AACCCCACAA CACCCAAGCC CCAGCCTTCC 
ACTTCCACAG ACCAGCCTGT CATCCCCAAA CCCACATCTC GGGCCCCTCG GGGCAGGACA 
CCTAGGTCTT CTGCCAAGAC TCCTGAACCA GCTGTCCCCA CAGCCTCTGA GCTCCAGCCT 
GCTGCCCCTA AAGACCAGCC TGTTGCTCCT GAGCTCACAT CTAGAGCCAC TCGGGGCAAG 
ACACAGAGGT CTATCAAGAC TTCCAAACCA GATATGCCCA CAACTCCCGA GCCCCAGCCT 
TCCACCTCCA CAGACCAGCC TGTCACCCCC AAACCCACGT CTCGGGCCCC TCGGGGCAGG 
ACACCTAGGT CTTCCGCCAA GACTCCTGAG CCAGTTGTCT CCACAGCCTC TGAGCTCCAG 
CCTTCTGCCC TCACAGATGA GCCTGTCACT CCTGAGCTCA CATCTAGGGC TACTCGGGGT 
CGGGCACAGA GGTCCTCTGT CAAAACCCCT GATCCAGTTA CCACCACAAC ACCTGAGCTC 
CAGCCTTCCA CCTCCACAGA CCAGCTTGTC ACCCCCAAAC GCACATCTCG GGCCCCTCGA 
GGCAGGACAC GTAGGTTGTC TGCCAAGACT CCTGAACCAG TTGTTCCCAC AGCCTCTGAG 
CTCCAGCCTT CTGCCCCTGC AGACCAGCCT GTTGGTCCTT GGGCCACTCA GTGTAGAAGA 
CATAGGTCTT CTGTCAAGAC CCCAGAACCA GTTGTCCCCA CAGTCCCTGA ACCTCAGCCT 
TCCACTTCTA AAGACCAGTC TGTCACTCCT GAGCCCACAT CTCAGGCCAC TGAGAGCCAA 
ACACATAGGT CCTCTATCAA GACATCCCAG CCAACGGAAC CCACAGCCTC TGACCTCAAA 
CCTTCCTCCC CCACAGACCA GCTTGTCACT CCCAAGGTCA TAGCTCAGGG TGGTCCAAGC 
AGGACAGGAA AGGCTTCCAC AGCAAGTGCT GTGCTGGTTC CTACTACCCC TGAATTCCAG 
TCTCCAGTCC CGTCAGAACA GCCTCTTCCC CCTGACCCCA TCCCTGAAGT CAACTGCAGC 
AGGAGGCTGA GGGCCACTAG GAAACATGGG TCTCCCACAG CTCATGTTCA TGAGCCCTGC 
ACCGTACCCC CTGAACCTAA CTCCCACTCT TCAAGGAACC AAAGACATGG GGCAGTGAAA 
GCAGCCAAGC CCCTTAGCAC CATTCCTGAG CCTGCCTTTG CCCAGCTCCC CGAGGCACCG 
CCTCACACTC CCCAGGTGCC AAAGGAGGAG GCAGCAGATG GATCAGGCTT CACCCCAGAG 
CCCCAGCCTA GGGCCTCTCA AAACCACAAG AGGCCTTCAG CTACTGCACA TTCACCTCCA 
CTTCAAAAAC GGCTCCAGAG AGGGAGAGTC CCTCAGAAGG CAGCATCCCT TAAGGAAGAA 
GAAAATCCAG TAGCGAGGCC GAGGAAGGAA GAGGGTGTAG TGATTCCAGG TCCAGGCAAG 
AGAAAGAGAG AGCAGACAGA GGAGGAGTCC CAGGGAAGAC CGAGCCGCAG CCTGCGACGG 
ACCAAACCGA TGCAGGAGTC CACGGCCCCC AAAGTGCTGT TCACCGGTGT GGTGGATGCT 
CGCGGAGAGA GGACGGTGCT GGCCCTGGGG GGCAGTTTGG CCAGCTCGGT GGCCGAGGCT 
TCTCACCTGG TGACTGATCG GATCCGCCGG ACGGTCAAGT TTCTGTGTGC CCTGGGCCGG 
GGCATCCCCA TCCTCTCCCT GGCCTGGCTG CATGAGTCCC GCAAGGCAGG CTGCTTCTTG 
CCCCCGGACG AATATTTGGT GACTGATCCT GAGCAGGAGA AGAACTTCGG CTTCAGCCTT 
CGGGAGGCCC TGAGCCGAGC TCAGGAGCGA AGGCTGCTGG AGGGCTATGA GATTCACGTG 
ACCCCCGGAG TCCAGCCACC GCCACCTCAG ATGGGAGAAA TCATCAGCTG CTGTGGAGGC 
GCCATCCTGC CCAGCATGCC CCGGTCCTAC AAGCCTCAGA GGTTCGTGAT CACATGTTCC 
CAGGACTTTC CTCGATGTGC TGTTCCATAT CGGGTTGGGC TGCCTATCCT CTCACCCGAA 
TTCCTGCTGA CGGGAGTACT GAAGCAGGAA GTCAAGCCAG AGGCCTTTGC CTTCTCCACT 
GTGGAAATGT CATCCACCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011956380.1
CDS	144..5204
Translation	MEVSHFPCLHFPFKIMEDTQILNWEVEEEEEVAESPNESLGYSLEPLAQLHIFSSSYGPEKDFPLYLGKNMIGRMPDCSVALPFSSISKQHAVIEISAWDKAPVLRDCGSLNGTQILRPPKVLSPGVSHRLRDRELILFADLPCQYHRLDVPRPFVSRGPLSVEETPRVQGGTQPSRLLLAEDSEEEVDSLLDKCVVKGPRTSSSATVVPESDEEGPSPAPDGPGPPSAFNLNSDTDEEESQESGAGEASSAARRGTAAETEQPEPVTAEIQIEKDQCSVKERNGDTEIERDVRNGVVPTGVILERSQPSGEDSDTDVSDESGPRRRLVGVRPKRAWSCNFIDSDTDGEDEGIPATPAVVPMKERQIFHEAGTQSPQAPGVARWQESPADGDTDIEEGEAPPDRSQASVVIDSNTDDEEEVSAALTLACLRESQADSEDLDLPATQCFVDRKNQSLEAAPSMEDEPTQAFLFPLPQEPGPSCCSSQATGSLDESWEALATQPFCPRESEASETQTVVTLLDTHASWPSPSRTAQQEQHPESPVHAEPLGMEGREMQTVEKEMGTPRETAERVIPEGGPPQEETKKLPSEGEREDVTGEEELIRGIQGSEKNQVFARDTQSQESDKKVKSASTGRGMEILKVEIETPKETQEKEREKQTLAGEIFESEAGKLVVERESEADGLEVKEPQELLDRSPQMGETEAGDQDQKGQASGLPSEPGAGAGDLQGLASDPVASGSQAGGGRGAPGSPRRQQRGDLNCEMPPAQKASRGDQESPDACLPPAAPEASAALPNSLIISQIQKHPAPQSLLFPSLAPLELPIPRTRQNESQEAPETPFSSELNSVHPEPKVRLQASSPVSSLPLEPHPTTPTGQPIALEPISGVSRSRTRSSFDVTASSVVPTAVALQPSTSTDKPVTPKPTLRAPRGRAQRSSVKTPEPNVTTDQPVAPELTAMATRGRAQRSSVKIPKPDNPTTPKPQPSTSTDQPVIPKPTSRAPRGRTPRSSAKTPEPAVPTASELQPAAPKDQPVAPELTSRATRGKTQRSIKTSKPDMPTTPEPQPSTSTDQPVTPKPTSRAPRGRTPRSSAKTPEPVVSTASELQPSALTDEPVTPELTSRATRGRAQRSSVKTPDPVTTTTPELQPSTSTDQLVTPKRTSRAPRGRTRRLSAKTPEPVVPTASELQPSAPADQPVGPWATQCRRHRSSVKTPEPVVPTVPEPQPSTSKDQSVTPEPTSQATESQTHRSSIKTSQPTEPTASDLKPSSPTDQLVTPKVIAQGGPSRTGKASTASAVLVPTTPEFQSPVPSEQPLPPDPIPEVNCSRRLRATRKHGSPTAHVHEPCTVPPEPNSHSSRNQRHGAVKAAKPLSTIPEPAFAQLPEAPPHTPQVPKEEAADGSGFTPEPQPRASQNHKRPSATAHSPPLQKRLQRGRVPQKAASLKEEENPVARPRKEEGVVIPGPGKRKREQTEEESQGRPSRSLRRTKPMQESTAPKVLFTGVVDARGERTVLALGGSLASSVAEASHLVTDRIRRTVKFLCALGRGIPILSLAWLHESRKAGCFLPPDEYLVTDPEQEKNFGFSLREALSRAQERRLLEGYEIHVTPGVQPPPPQMGEIISCCGGAILPSMPRSYKPQRFVITCSQDFPRCAVPYRVGLPILSPEFLLTGVLKQEVKPEAFAFSTVEMSST

Target ORF information:

RefSeq Version	XM_012100990.2
Organism	Ovis aries(sheep)
Definition	Ovis aries mediator of DNA damage checkpoint 1 (MDC1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012100990.2

ORF Insert Sequence:

ATGGAGGTGT CCCACTTTCC TTGTCTACAT TTTCCCTTTA AGATCATGGA AGACACCCAG 
ATTTTAAACT GGGAAGTTGA AGAAGAGGAG GAGGTTGCAG AGAGCCCCAA TGAATCTCTG 
GGGTATAGCT TGGAGCCCCT AGCACAGCTG CATATCTTCA GTAGTTCCTA TGGACCAGAA 
AAAGACTTTC CACTCTACCT TGGAAAGAAC ATGATAGGCC GAATGCCTGA TTGCTCTGTG 
GCCCTGCCCT TTTCATCAAT ATCCAAACAA CATGCAGTGA TTGAAATCTC TGCCTGGGAC 
AAGGCGCCTG TCCTTCGGGA TTGCGGGAGC CTCAATGGCA CTCAAATCCT GCGGCCTCCT 
AAGGTCCTGA GCCCTGGGGT GAGTCATCGT TTGCGGGACC GGGAGTTGAT TCTCTTTGCT 
GACTTGCCCT GCCAGTACCA TCGCTTGGAT GTCCCCCGGC CTTTTGTCTC CCGGGGCCCT 
CTAAGTGTAG AGGAGACACC CAGGGTACAG GGAGGAACTC AACCCTCCAG GCTTCTGTTG 
GCTGAGGACT CAGAGGAAGA AGTAGATTCC CTTTTGGACA AGTGTGTGGT GAAAGGACCA 
AGGACCTCCT CTTCGGCAAC AGTCGTTCCA GAGAGTGATG AAGAAGGGCC TTCCCCTGCC 
CCAGATGGGC CCGGGCCACC TTCTGCCTTC AACTTGAACA GCGACACGGA TGAGGAAGAA 
AGTCAGGAAT CAGGAGCAGG GGAGGCCTCT TCAGCTGCCA GAAGAGGTAC CGCTGCAGAG 
ACGGAACAGC CTGAACCTGT CACAGCCGAG ATCCAGATTG AGAAGGATCA GTGTTCCGTG 
AAGGAGAGGA ACGGGGACAC AGAAATCGAG AGGGATGTGA GGAATGGGGT GGTTCCGACT 
GGAGTGATTC TGGAGAGGAG CCAGCCTTCT GGGGAGGACA GTGACACAGA TGTGAGTGAT 
GAGAGTGGGC CTCGAAGAAG GCTGGTTGGA GTCCGTCCGA AAAGGGCCTG GTCTTGTAAC 
TTCATAGACA GTGATACCGA TGGAGAGGAC GAGGGGATCC CTGCTACCCC AGCAGTGGTT 
CCCATGAAGG AGAGGCAGAT CTTCCACGAA GCTGGTACAC AGAGCCCCCA GGCACCTGGT 
GTGGCACGTT GGCAGGAGAG CCCAGCTGAT GGTGATACAG ATATAGAGGA GGGGGAGGCC 
CCCCCAGACA GAAGCCAAGC CTCCGTGGTG ATCGACAGCA ATACAGACGA TGAGGAAGAA 
GTCTCAGCAG CACTGACACT GGCATGTCTA AGAGAGAGCC AGGCTGATTC TGAAGATCTG 
GACCTACCAG CTACCCAGTG CTTTGTAGAC AGAAAGAATC AGAGCCTGGA AGCAGCCCCC 
AGCATGGAGG ATGAGCCCAC CCAGGCCTTC CTGTTTCCTC TGCCCCAAGA GCCTGGCCCT 
TCCTGTTGCA GCTCCCAGGC CACAGGTTCC TTGGATGAGT CATGGGAGGC CTTGGCGACA 
CAGCCATTCT GTCCGAGAGA ATCTGAGGCC TCCGAGACCC AAACCGTTGT CACCCTCCTT 
GACACCCATG CATCTTGGCC CTCTCCATCT AGGACAGCAC AGCAAGAGCA ACATCCAGAG 
AGCCCAGTCC ATGCAGAGCC ACTGGGGATG GAAGGCAGAG AGATGCAGAC TGTGGAGAAA 
GAAATGGGTA CCCCAAGAGA AACAGCAGAG AGGGTGATCC CTGAGGGAGG GCCACCGCAG 
GAGGAAACCA AGAAACTGCC CTCAGAAGGA GAGAGGGAAG ATGTGACGGG AGAGGAAGAA 
TTAATCCGGG GGATACAGGG CAGTGAAAAA AATCAGGTGT TTGCTAGAGA TACTCAGAGC 
CAAGAATCTG ACAAAAAAGT GAAAAGTGCA AGTACTGGAA GGGGAATGGA GATTTTAAAG 
GTAGAAATTG AGACACCCAA GGAAACACAA GAGAAAGAGA GAGAAAAGCA GACTCTCGCA 
GGGGAAATAT TTGAGAGTGA AGCAGGGAAA CTGGTAGTAG AGAGAGAGAG TGAGGCAGAT 
GGGTTAGAAG TCAAGGAACC CCAAGAGCTA CTGGACAGAA GCCCACAGAT GGGGGAGACA 
GAGGCGGGGG ACCAGGACCA GAAAGGCCAA GCCTCTGGTC TGCCATCAGA GCCTGGAGCA 
GGGGCAGGAG ACCTTCAGGG ACTTGCTTCA GACCCAGTAG CTTCTGGGAG CCAGGCAGGT 
GGAGGAAGGG GAGCCCCAGG GAGCCCCAGG AGGCAGCAGA GAGGTGACTT GAATTGCGAG 
ATGCCACCTG CTCAGAAGGC TTCCAGGGGT GATCAGGAAT CCCCAGATGC TTGTCTGCCT 
CCTGCAGCGC CTGAAGCCTC AGCCGCACTC CCAAATTCCC TCATCATCTC TCAAATCCAA 
AAACATCCCG CACCTCAGTC CCTCCTTTTT CCCTCTCTAG CTCCTTTAGA ACTGCCTATT 
CCCAGGACCA GACAAAATGA GAGTCAGGAA GCTCCAGAGA CTCCCTTCTC CTCAGAGCTG 
AACTCTGTCC ACCCAGAACC CAAAGTCAGG CTCCAGGCGT CCTCTCCAGT TTCTTCTCTA 
CCCCTTGAGC CCCACCCTAC CACCCCCACA GGCCAGCCTA TTGCCCTTGA ACCCATATCT 
GGGGTCTCTC GGAGCAGGAC ACGTAGTTCC TTTGATGTAA CTGCCTCATC AGTTGTCCCC 
ACAGCCGTTG CACTGCAGCC ATCCACCTCC ACAGACAAGC CTGTCACCCC TAAGCCCACA 
CTTCGGGCCC CTCGGGGCAG GGCACAGAGG TCTTCTGTCA AAACCCCTGA ACCCAATGTC 
ACCACAGACC AGCCTGTTGC CCCTGAGCTC ACAGCTATGG CAACTCGGGG CAGGGCACAG 
AGGTCTTCTG TCAAGATTCC CAAACCAGAT AACCCCACAA CACCCAAGCC CCAGCCTTCC 
ACTTCCACAG ACCAGCCTGT CATCCCCAAA CCCACATCTC GGGCCCCTCG GGGCAGGACA 
CCTAGGTCTT CTGCCAAGAC TCCTGAACCA GCTGTCCCCA CAGCCTCTGA GCTCCAGCCT 
GCTGCCCCTA AAGACCAGCC TGTTGCTCCT GAGCTCACAT CTAGAGCCAC TCGGGGCAAG 
ACACAGAGGT CTATCAAGAC TTCCAAACCA GATATGCCCA CAACTCCCGA GCCCCAGCCT 
TCCACCTCCA CAGACCAGCC TGTCACCCCC AAACCCACGT CTCGGGCCCC TCGGGGCAGG 
ACACCTAGGT CTTCCGCCAA GACTCCTGAG CCAGTTGTCT CCACAGCCTC TGAGCTCCAG 
CCTTCTGCCC TCACAGATGA GCCTGTCACT CCTGAGCTCA CATCTAGGGC TACTCGGGGT 
CGGGCACAGA GGTCCTCTGT CAAAACCCCT GATCCAGTTA CCACCACAAC ACCTGAGCTC 
CAGCCTTCCA CCTCCACAGA CCAGCTTGTC ACCCCCAAAC GCACATCTCG GGCCCCTCGA 
GGCAGGACAC GTAGGTTGTC TGCCAAGACT CCTGAACCAG TTGTTCCCAC AGCCTCTGAG 
CTCCAGCCTT CTGCCCCTGC AGACCAGCCT GTTGGTCCTT GGGCCACTCA GTGTAGAAGA 
CATAGGTCTT CTGTCAAGAC CCCAGAACCA GTTGTCCCCA CAGTCCCTGA ACCTCAGCCT 
TCCACTTCTA AAGACCAGTC TGTCACTCCT GAGCCCACAT CTCAGGCCAC TGAGAGCCAA 
ACACATAGGT CCTCTATCAA GACATCCCAG CCAACGGAAC CCACAGCCTC TGACCTCAAA 
CCTTCCTCCC CCACAGACCA GCTTGTCACT CCCAAGGTCA TAGCTCAGGG TGGTCCAAGC 
AGGACAGGAA AGGCTTCCAC AGCAAGTGCT GTGCTGGTTC CTACTACCCC TGAATTCCAG 
TCTCCAGTCC CGTCAGAACA GCCTCTTCCC CCTGACCCCA TCCCTGAAGT CAACTGCAGC 
AGGAGGCTGA GGGCCACTAG GAAACATGGG TCTCCCACAG CTCATGTTCA TGAGCCCTGC 
ACCGTACCCC CTGAACCTAA CTCCCACTCT TCAAGGAACC AAAGACATGG GGCAGTGAAA 
GCAGCCAAGC CCCTTAGCAC CATTCCTGAG CCTGCCTTTG CCCAGCTCCC CGAGGCACCG 
CCTCACACTC CCCAGGTGCC AAAGGAGGAG GCAGCAGATG GATCAGGCTT CACCCCAGAG 
CCCCAGCCTA GGGCCTCTCA AAACCACAAG AGGCCTTCAG CTACTGCACA TTCACCTCCA 
CTTCAAAAAC GGCTCCAGAG AGGGAGAGTC CCTCAGAAGG CAGCATCCCT TAAGGAAGAA 
GAAAATCCAG TAGCGAGGCC GAGGAAGGAA GAGGGTGTAG TGATTCCAGG TCCAGGCAAG 
AGAAAGAGAG AGCAGACAGA GGAGGAGTCC CAGGGAAGAC CGAGCCGCAG CCTGCGACGG 
ACCAAACCGA TGCAGGAGTC CACGGCCCCC AAAGTGCTGT TCACCGGTGT GGTGGATGCT 
CGCGGAGAGA GGACGGTGCT GGCCCTGGGG GGCAGTTTGG CCAGCTCGGT GGCCGAGGCT 
TCTCACCTGG TGACTGATCG GATCCGCCGG ACGGTCAAGT TTCTGTGTGC CCTGGGCCGG 
GGCATCCCCA TCCTCTCCCT GGCCTGGCTG CATGAGTCCC GCAAGGCAGG CTGCTTCTTG 
CCCCCGGACG AATATTTGGT GACTGATCCT GAGCAGGAGA AGAACTTCGG CTTCAGCCTT 
CGGGAGGCCC TGAGCCGAGC TCAGGAGCGA AGGCTGCTGG AGGGCTATGA GATTCACGTG 
ACCCCCGGAG TCCAGCCACC GCCACCTCAG ATGGGAGAAA TCATCAGCTG CTGTGGAGGC 
GCCATCCTGC CCAGCATGCC CCGGTCCTAC AAGCCTCAGA GGTTCGTGAT CACATGTTCC 
CAGGACTTTC CTCGATGTGC TGTTCCATAT CGGGTTGGGC TGCCTATCCT CTCACCCGAA 
TTCCTGCTGA CGGGAGTACT GAAGCAGGAA GTCAAGCCAG AGGCCTTTGC CTTCTCCACT 
GTGGAAATGT CATCCACCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MDC1 ( XM_012100991.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_011956381.1 Ovis aries mediator of DNA damage checkpoint 1 (MDC1), transcript variant X7, mRNA.

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CloneID	OSm28031
Clone ID Related Accession (Same CDS sequence)	XM_012100991.2
Accession Version	XM_012100991.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5058bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries(sheep)
Product	mediator of DNA damage checkpoint protein 1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014639029.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012100991.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGTGT CCCACTTTCC TTGTCTACAT TTTCCCTTTA AGATCATGGA AGACACCCAG 
ATTTTAAACT GGGAAGTTGA AGAAGAGGAG GAGGTTGCAG AGAGCCCCAA TGAATCTCTG 
GGGTATAGCT TGGAGCCCCT AGCACAGCTG CATATCTTCA GTAGTTCCTA TGGACCAGAA 
AAAGACTTTC CACTCTACCT TGGAAAGAAC ATGATAGGCC GAATGCCTGA TTGCTCTGTG 
GCCCTGCCCT TTTCATCAAT ATCCAAACAA CATGCAGTGA TTGAAATCTC TGCCTGGGAC 
AAGGCGCCTG TCCTTCGGGA TTGCGGGAGC CTCAATGGCA CTCAAATCCT GCGGCCTCCT 
AAGGTCCTGA GCCCTGGGGT GAGTCATCGT TTGCGGGACC GGGAGTTGAT TCTCTTTGCT 
GACTTGCCCT GCCAGTACCA TCGCTTGGAT GTCCCCCGGC CTTTTGTCTC CCGGGGCCCT 
CTAAGTGTAG AGGAGACACC CAGGGTACAG GGAGGAACTC AACCCTCCAG GCTTCTGTTG 
GCTGAGGACT CAGAGGAAGA AGTAGATTCC CTTTTGGACA AGTGTGTGGT GAAAGGACCA 
AGGACCTCCT CTTCGGCAAC AGTCGTTCCA GAGAGTGATG AAGAAGGGCC TTCCCCTGCC 
CCAGATGGGC CCGGGCCACC TTCTGCCTTC AACTTGAACA GCGACACGGA TGAGGAAGAA 
AGTCAGGAAT CAGGAGCAGG GGAGGCCTCT TCAGCTGCCA GAAGAGGTAC CGCTGCAGAG 
ACGGAACAGC CTGAACCTGT CACAGCCGAG ATCCAGATTG AGAAGGATCA GTGTTCCGTG 
AAGGAGAGGA ACGGGGACAC AGAAATCGAG AGGGATGTGA GGAATGGGGT GGTTCCGACT 
GGAGTGATTC TGGAGAGGAG CCAGCCTTCT GGGGAGGACA GTGACACAGA TGTGAGTGAT 
GAGAGTGGGC CTCGAAGAAG GCTGGTTGGA GTCCGTCCGA AAAGGGCCTG GTCTTGTAAC 
TTCATAGACA GTGATACCGA TGGAGAGGAC GAGGGGATCC CTGCTACCCC AGCAGTGGTT 
CCCATGAAGG AGAGGCAGAT CTTCCACGAA GCTGGTACAC AGAGCCCCCA GGCACCTGGT 
GTGGCACGTT GGCAGGAGAG CCCAGCTGAT GGTGATACAG ATATAGAGGA GGGGGAGGCC 
CCCCCAGACA GAAGCCAAGC CTCCGTGGTG ATCGACAGCA ATACAGACGA TGAGGAAGAA 
GTCTCAGCAG CACTGACACT GGCATGTCTA AGAGAGAGCC AGGCTGATTC TGAAGATCTG 
GACCTACCAG CTACCCAGTG CTTTGTAGAC AGAAAGAATC AGAGCCTGGA AGCCCCCAGC 
ATGGAGGATG AGCCCACCCA GGCCTTCCTG TTTCCTCTGC CCCAAGAGCC TGGCCCTTCC 
TGTTGCAGCT CCCAGGCCAC AGGTTCCTTG GATGAGTCAT GGGAGGCCTT GGCGACACAG 
CCATTCTGTC CGAGAGAATC TGAGGCCTCC GAGACCCAAA CCGTTGTCAC CCTCCTTGAC 
ACCCATGCAT CTTGGCCCTC TCCATCTAGG ACAGCACAGC AAGAGCAACA TCCAGAGAGC 
CCAGTCCATG CAGAGCCACT GGGGATGGAA GGCAGAGAGA TGCAGACTGT GGAGAAAGAA 
ATGGGTACCC CAAGAGAAAC AGCAGAGAGG GTGATCCCTG AGGGAGGGCC ACCGCAGGAG 
GAAACCAAGA AACTGCCCTC AGAAGGAGAG AGGGAAGATG TGACGGGAGA GGAAGAATTA 
ATCCGGGGGA TACAGGGCAG TGAAAAAAAT CAGGTGTTTG CTAGAGATAC TCAGAGCCAA 
GAATCTGACA AAAAAGTGAA AAGTGCAAGT ACTGGAAGGG GAATGGAGAT TTTAAAGGTA 
GAAATTGAGA CACCCAAGGA AACACAAGAG AAAGAGAGAG AAAAGCAGAC TCTCGCAGGG 
GAAATATTTG AGAGTGAAGC AGGGAAACTG GTAGTAGAGA GAGAGAGTGA GGCAGATGGG 
TTAGAAGTCA AGGAACCCCA AGAGCTACTG GACAGAAGCC CACAGATGGG GGAGACAGAG 
GCGGGGGACC AGGACCAGAA AGGCCAAGCC TCTGGTCTGC CATCAGAGCC TGGAGCAGGG 
GCAGGAGACC TTCAGGGACT TGCTTCAGAC CCAGTAGCTT CTGGGAGCCA GGCAGGTGGA 
GGAAGGGGAG CCCCAGGGAG CCCCAGGAGG CAGCAGAGAG GTGACTTGAA TTGCGAGATG 
CCACCTGCTC AGAAGGCTTC CAGGGGTGAT CAGGAATCCC CAGATGCTTG TCTGCCTCCT 
GCAGCGCCTG AAGCCTCAGC CGCACTCCCA AATTCCCTCA TCATCTCTCA AATCCAAAAA 
CATCCCGCAC CTCAGTCCCT CCTTTTTCCC TCTCTAGCTC CTTTAGAACT GCCTATTCCC 
AGGACCAGAC AAAATGAGAG TCAGGAAGCT CCAGAGACTC CCTTCTCCTC AGAGCTGAAC 
TCTGTCCACC CAGAACCCAA AGTCAGGCTC CAGGCGTCCT CTCCAGTTTC TTCTCTACCC 
CTTGAGCCCC ACCCTACCAC CCCCACAGGC CAGCCTATTG CCCTTGAACC CATATCTGGG 
GTCTCTCGGA GCAGGACACG TAGTTCCTTT GATGTAACTG CCTCATCAGT TGTCCCCACA 
GCCGTTGCAC TGCAGCCATC CACCTCCACA GACAAGCCTG TCACCCCTAA GCCCACACTT 
CGGGCCCCTC GGGGCAGGGC ACAGAGGTCT TCTGTCAAAA CCCCTGAACC CAATGTCACC 
ACAGACCAGC CTGTTGCCCC TGAGCTCACA GCTATGGCAA CTCGGGGCAG GGCACAGAGG 
TCTTCTGTCA AGATTCCCAA ACCAGATAAC CCCACAACAC CCAAGCCCCA GCCTTCCACT 
TCCACAGACC AGCCTGTCAT CCCCAAACCC ACATCTCGGG CCCCTCGGGG CAGGACACCT 
AGGTCTTCTG CCAAGACTCC TGAACCAGCT GTCCCCACAG CCTCTGAGCT CCAGCCTGCT 
GCCCCTAAAG ACCAGCCTGT TGCTCCTGAG CTCACATCTA GAGCCACTCG GGGCAAGACA 
CAGAGGTCTA TCAAGACTTC CAAACCAGAT ATGCCCACAA CTCCCGAGCC CCAGCCTTCC 
ACCTCCACAG ACCAGCCTGT CACCCCCAAA CCCACGTCTC GGGCCCCTCG GGGCAGGACA 
CCTAGGTCTT CCGCCAAGAC TCCTGAGCCA GTTGTCTCCA CAGCCTCTGA GCTCCAGCCT 
TCTGCCCTCA CAGATGAGCC TGTCACTCCT GAGCTCACAT CTAGGGCTAC TCGGGGTCGG 
GCACAGAGGT CCTCTGTCAA AACCCCTGAT CCAGTTACCA CCACAACACC TGAGCTCCAG 
CCTTCCACCT CCACAGACCA GCTTGTCACC CCCAAACGCA CATCTCGGGC CCCTCGAGGC 
AGGACACGTA GGTTGTCTGC CAAGACTCCT GAACCAGTTG TTCCCACAGC CTCTGAGCTC 
CAGCCTTCTG CCCCTGCAGA CCAGCCTGTT GGTCCTTGGG CCACTCAGTG TAGAAGACAT 
AGGTCTTCTG TCAAGACCCC AGAACCAGTT GTCCCCACAG TCCCTGAACC TCAGCCTTCC 
ACTTCTAAAG ACCAGTCTGT CACTCCTGAG CCCACATCTC AGGCCACTGA GAGCCAAACA 
CATAGGTCCT CTATCAAGAC ATCCCAGCCA ACGGAACCCA CAGCCTCTGA CCTCAAACCT 
TCCTCCCCCA CAGACCAGCT TGTCACTCCC AAGGTCATAG CTCAGGGTGG TCCAAGCAGG 
ACAGGAAAGG CTTCCACAGC AAGTGCTGTG CTGGTTCCTA CTACCCCTGA ATTCCAGTCT 
CCAGTCCCGT CAGAACAGCC TCTTCCCCCT GACCCCATCC CTGAAGTCAA CTGCAGCAGG 
AGGCTGAGGG CCACTAGGAA ACATGGGTCT CCCACAGCTC ATGTTCATGA GCCCTGCACC 
GTACCCCCTG AACCTAACTC CCACTCTTCA AGGAACCAAA GACATGGGGC AGTGAAAGCA 
GCCAAGCCCC TTAGCACCAT TCCTGAGCCT GCCTTTGCCC AGCTCCCCGA GGCACCGCCT 
CACACTCCCC AGGTGCCAAA GGAGGAGGCA GCAGATGGAT CAGGCTTCAC CCCAGAGCCC 
CAGCCTAGGG CCTCTCAAAA CCACAAGAGG CCTTCAGCTA CTGCACATTC ACCTCCACTT 
CAAAAACGGC TCCAGAGAGG GAGAGTCCCT CAGAAGGCAG CATCCCTTAA GGAAGAAGAA 
AATCCAGTAG CGAGGCCGAG GAAGGAAGAG GGTGTAGTGA TTCCAGGTCC AGGCAAGAGA 
AAGAGAGAGC AGACAGAGGA GGAGTCCCAG GGAAGACCGA GCCGCAGCCT GCGACGGACC 
AAACCGATGC AGGAGTCCAC GGCCCCCAAA GTGCTGTTCA CCGGTGTGGT GGATGCTCGC 
GGAGAGAGGA CGGTGCTGGC CCTGGGGGGC AGTTTGGCCA GCTCGGTGGC CGAGGCTTCT 
CACCTGGTGA CTGATCGGAT CCGCCGGACG GTCAAGTTTC TGTGTGCCCT GGGCCGGGGC 
ATCCCCATCC TCTCCCTGGC CTGGCTGCAT GAGTCCCGCA AGGCAGGCTG CTTCTTGCCC 
CCGGACGAAT ATTTGGTGAC TGATCCTGAG CAGGAGAAGA ACTTCGGCTT CAGCCTTCGG 
GAGGCCCTGA GCCGAGCTCA GGAGCGAAGG CTGCTGGAGG GCTATGAGAT TCACGTGACC 
CCCGGAGTCC AGCCACCGCC ACCTCAGATG GGAGAAATCA TCAGCTGCTG TGGAGGCGCC 
ATCCTGCCCA GCATGCCCCG GTCCTACAAG CCTCAGAGGT TCGTGATCAC ATGTTCCCAG 
GACTTTCCTC GATGTGCTGT TCCATATCGG GTTGGGCTGC CTATCCTCTC ACCCGAATTC 
CTGCTGACGG GAGTACTGAA GCAGGAAGTC AAGCCAGAGG CCTTTGCCTT CTCCACTGTG 
GAAATGTCAT CCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011956381.1
CDS	144..5201
Translation	MEVSHFPCLHFPFKIMEDTQILNWEVEEEEEVAESPNESLGYSLEPLAQLHIFSSSYGPEKDFPLYLGKNMIGRMPDCSVALPFSSISKQHAVIEISAWDKAPVLRDCGSLNGTQILRPPKVLSPGVSHRLRDRELILFADLPCQYHRLDVPRPFVSRGPLSVEETPRVQGGTQPSRLLLAEDSEEEVDSLLDKCVVKGPRTSSSATVVPESDEEGPSPAPDGPGPPSAFNLNSDTDEEESQESGAGEASSAARRGTAAETEQPEPVTAEIQIEKDQCSVKERNGDTEIERDVRNGVVPTGVILERSQPSGEDSDTDVSDESGPRRRLVGVRPKRAWSCNFIDSDTDGEDEGIPATPAVVPMKERQIFHEAGTQSPQAPGVARWQESPADGDTDIEEGEAPPDRSQASVVIDSNTDDEEEVSAALTLACLRESQADSEDLDLPATQCFVDRKNQSLEAPSMEDEPTQAFLFPLPQEPGPSCCSSQATGSLDESWEALATQPFCPRESEASETQTVVTLLDTHASWPSPSRTAQQEQHPESPVHAEPLGMEGREMQTVEKEMGTPRETAERVIPEGGPPQEETKKLPSEGEREDVTGEEELIRGIQGSEKNQVFARDTQSQESDKKVKSASTGRGMEILKVEIETPKETQEKEREKQTLAGEIFESEAGKLVVERESEADGLEVKEPQELLDRSPQMGETEAGDQDQKGQASGLPSEPGAGAGDLQGLASDPVASGSQAGGGRGAPGSPRRQQRGDLNCEMPPAQKASRGDQESPDACLPPAAPEASAALPNSLIISQIQKHPAPQSLLFPSLAPLELPIPRTRQNESQEAPETPFSSELNSVHPEPKVRLQASSPVSSLPLEPHPTTPTGQPIALEPISGVSRSRTRSSFDVTASSVVPTAVALQPSTSTDKPVTPKPTLRAPRGRAQRSSVKTPEPNVTTDQPVAPELTAMATRGRAQRSSVKIPKPDNPTTPKPQPSTSTDQPVIPKPTSRAPRGRTPRSSAKTPEPAVPTASELQPAAPKDQPVAPELTSRATRGKTQRSIKTSKPDMPTTPEPQPSTSTDQPVTPKPTSRAPRGRTPRSSAKTPEPVVSTASELQPSALTDEPVTPELTSRATRGRAQRSSVKTPDPVTTTTPELQPSTSTDQLVTPKRTSRAPRGRTRRLSAKTPEPVVPTASELQPSAPADQPVGPWATQCRRHRSSVKTPEPVVPTVPEPQPSTSKDQSVTPEPTSQATESQTHRSSIKTSQPTEPTASDLKPSSPTDQLVTPKVIAQGGPSRTGKASTASAVLVPTTPEFQSPVPSEQPLPPDPIPEVNCSRRLRATRKHGSPTAHVHEPCTVPPEPNSHSSRNQRHGAVKAAKPLSTIPEPAFAQLPEAPPHTPQVPKEEAADGSGFTPEPQPRASQNHKRPSATAHSPPLQKRLQRGRVPQKAASLKEEENPVARPRKEEGVVIPGPGKRKREQTEEESQGRPSRSLRRTKPMQESTAPKVLFTGVVDARGERTVLALGGSLASSVAEASHLVTDRIRRTVKFLCALGRGIPILSLAWLHESRKAGCFLPPDEYLVTDPEQEKNFGFSLREALSRAQERRLLEGYEIHVTPGVQPPPPQMGEIISCCGGAILPSMPRSYKPQRFVITCSQDFPRCAVPYRVGLPILSPEFLLTGVLKQEVKPEAFAFSTVEMSST

Target ORF information:

RefSeq Version	XM_012100991.2
Organism	Ovis aries(sheep)
Definition	Ovis aries mediator of DNA damage checkpoint 1 (MDC1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012100991.2

ORF Insert Sequence:

ATGGAGGTGT CCCACTTTCC TTGTCTACAT TTTCCCTTTA AGATCATGGA AGACACCCAG 
ATTTTAAACT GGGAAGTTGA AGAAGAGGAG GAGGTTGCAG AGAGCCCCAA TGAATCTCTG 
GGGTATAGCT TGGAGCCCCT AGCACAGCTG CATATCTTCA GTAGTTCCTA TGGACCAGAA 
AAAGACTTTC CACTCTACCT TGGAAAGAAC ATGATAGGCC GAATGCCTGA TTGCTCTGTG 
GCCCTGCCCT TTTCATCAAT ATCCAAACAA CATGCAGTGA TTGAAATCTC TGCCTGGGAC 
AAGGCGCCTG TCCTTCGGGA TTGCGGGAGC CTCAATGGCA CTCAAATCCT GCGGCCTCCT 
AAGGTCCTGA GCCCTGGGGT GAGTCATCGT TTGCGGGACC GGGAGTTGAT TCTCTTTGCT 
GACTTGCCCT GCCAGTACCA TCGCTTGGAT GTCCCCCGGC CTTTTGTCTC CCGGGGCCCT 
CTAAGTGTAG AGGAGACACC CAGGGTACAG GGAGGAACTC AACCCTCCAG GCTTCTGTTG 
GCTGAGGACT CAGAGGAAGA AGTAGATTCC CTTTTGGACA AGTGTGTGGT GAAAGGACCA 
AGGACCTCCT CTTCGGCAAC AGTCGTTCCA GAGAGTGATG AAGAAGGGCC TTCCCCTGCC 
CCAGATGGGC CCGGGCCACC TTCTGCCTTC AACTTGAACA GCGACACGGA TGAGGAAGAA 
AGTCAGGAAT CAGGAGCAGG GGAGGCCTCT TCAGCTGCCA GAAGAGGTAC CGCTGCAGAG 
ACGGAACAGC CTGAACCTGT CACAGCCGAG ATCCAGATTG AGAAGGATCA GTGTTCCGTG 
AAGGAGAGGA ACGGGGACAC AGAAATCGAG AGGGATGTGA GGAATGGGGT GGTTCCGACT 
GGAGTGATTC TGGAGAGGAG CCAGCCTTCT GGGGAGGACA GTGACACAGA TGTGAGTGAT 
GAGAGTGGGC CTCGAAGAAG GCTGGTTGGA GTCCGTCCGA AAAGGGCCTG GTCTTGTAAC 
TTCATAGACA GTGATACCGA TGGAGAGGAC GAGGGGATCC CTGCTACCCC AGCAGTGGTT 
CCCATGAAGG AGAGGCAGAT CTTCCACGAA GCTGGTACAC AGAGCCCCCA GGCACCTGGT 
GTGGCACGTT GGCAGGAGAG CCCAGCTGAT GGTGATACAG ATATAGAGGA GGGGGAGGCC 
CCCCCAGACA GAAGCCAAGC CTCCGTGGTG ATCGACAGCA ATACAGACGA TGAGGAAGAA 
GTCTCAGCAG CACTGACACT GGCATGTCTA AGAGAGAGCC AGGCTGATTC TGAAGATCTG 
GACCTACCAG CTACCCAGTG CTTTGTAGAC AGAAAGAATC AGAGCCTGGA AGCCCCCAGC 
ATGGAGGATG AGCCCACCCA GGCCTTCCTG TTTCCTCTGC CCCAAGAGCC TGGCCCTTCC 
TGTTGCAGCT CCCAGGCCAC AGGTTCCTTG GATGAGTCAT GGGAGGCCTT GGCGACACAG 
CCATTCTGTC CGAGAGAATC TGAGGCCTCC GAGACCCAAA CCGTTGTCAC CCTCCTTGAC 
ACCCATGCAT CTTGGCCCTC TCCATCTAGG ACAGCACAGC AAGAGCAACA TCCAGAGAGC 
CCAGTCCATG CAGAGCCACT GGGGATGGAA GGCAGAGAGA TGCAGACTGT GGAGAAAGAA 
ATGGGTACCC CAAGAGAAAC AGCAGAGAGG GTGATCCCTG AGGGAGGGCC ACCGCAGGAG 
GAAACCAAGA AACTGCCCTC AGAAGGAGAG AGGGAAGATG TGACGGGAGA GGAAGAATTA 
ATCCGGGGGA TACAGGGCAG TGAAAAAAAT CAGGTGTTTG CTAGAGATAC TCAGAGCCAA 
GAATCTGACA AAAAAGTGAA AAGTGCAAGT ACTGGAAGGG GAATGGAGAT TTTAAAGGTA 
GAAATTGAGA CACCCAAGGA AACACAAGAG AAAGAGAGAG AAAAGCAGAC TCTCGCAGGG 
GAAATATTTG AGAGTGAAGC AGGGAAACTG GTAGTAGAGA GAGAGAGTGA GGCAGATGGG 
TTAGAAGTCA AGGAACCCCA AGAGCTACTG GACAGAAGCC CACAGATGGG GGAGACAGAG 
GCGGGGGACC AGGACCAGAA AGGCCAAGCC TCTGGTCTGC CATCAGAGCC TGGAGCAGGG 
GCAGGAGACC TTCAGGGACT TGCTTCAGAC CCAGTAGCTT CTGGGAGCCA GGCAGGTGGA 
GGAAGGGGAG CCCCAGGGAG CCCCAGGAGG CAGCAGAGAG GTGACTTGAA TTGCGAGATG 
CCACCTGCTC AGAAGGCTTC CAGGGGTGAT CAGGAATCCC CAGATGCTTG TCTGCCTCCT 
GCAGCGCCTG AAGCCTCAGC CGCACTCCCA AATTCCCTCA TCATCTCTCA AATCCAAAAA 
CATCCCGCAC CTCAGTCCCT CCTTTTTCCC TCTCTAGCTC CTTTAGAACT GCCTATTCCC 
AGGACCAGAC AAAATGAGAG TCAGGAAGCT CCAGAGACTC CCTTCTCCTC AGAGCTGAAC 
TCTGTCCACC CAGAACCCAA AGTCAGGCTC CAGGCGTCCT CTCCAGTTTC TTCTCTACCC 
CTTGAGCCCC ACCCTACCAC CCCCACAGGC CAGCCTATTG CCCTTGAACC CATATCTGGG 
GTCTCTCGGA GCAGGACACG TAGTTCCTTT GATGTAACTG CCTCATCAGT TGTCCCCACA 
GCCGTTGCAC TGCAGCCATC CACCTCCACA GACAAGCCTG TCACCCCTAA GCCCACACTT 
CGGGCCCCTC GGGGCAGGGC ACAGAGGTCT TCTGTCAAAA CCCCTGAACC CAATGTCACC 
ACAGACCAGC CTGTTGCCCC TGAGCTCACA GCTATGGCAA CTCGGGGCAG GGCACAGAGG 
TCTTCTGTCA AGATTCCCAA ACCAGATAAC CCCACAACAC CCAAGCCCCA GCCTTCCACT 
TCCACAGACC AGCCTGTCAT CCCCAAACCC ACATCTCGGG CCCCTCGGGG CAGGACACCT 
AGGTCTTCTG CCAAGACTCC TGAACCAGCT GTCCCCACAG CCTCTGAGCT CCAGCCTGCT 
GCCCCTAAAG ACCAGCCTGT TGCTCCTGAG CTCACATCTA GAGCCACTCG GGGCAAGACA 
CAGAGGTCTA TCAAGACTTC CAAACCAGAT ATGCCCACAA CTCCCGAGCC CCAGCCTTCC 
ACCTCCACAG ACCAGCCTGT CACCCCCAAA CCCACGTCTC GGGCCCCTCG GGGCAGGACA 
CCTAGGTCTT CCGCCAAGAC TCCTGAGCCA GTTGTCTCCA CAGCCTCTGA GCTCCAGCCT 
TCTGCCCTCA CAGATGAGCC TGTCACTCCT GAGCTCACAT CTAGGGCTAC TCGGGGTCGG 
GCACAGAGGT CCTCTGTCAA AACCCCTGAT CCAGTTACCA CCACAACACC TGAGCTCCAG 
CCTTCCACCT CCACAGACCA GCTTGTCACC CCCAAACGCA CATCTCGGGC CCCTCGAGGC 
AGGACACGTA GGTTGTCTGC CAAGACTCCT GAACCAGTTG TTCCCACAGC CTCTGAGCTC 
CAGCCTTCTG CCCCTGCAGA CCAGCCTGTT GGTCCTTGGG CCACTCAGTG TAGAAGACAT 
AGGTCTTCTG TCAAGACCCC AGAACCAGTT GTCCCCACAG TCCCTGAACC TCAGCCTTCC 
ACTTCTAAAG ACCAGTCTGT CACTCCTGAG CCCACATCTC AGGCCACTGA GAGCCAAACA 
CATAGGTCCT CTATCAAGAC ATCCCAGCCA ACGGAACCCA CAGCCTCTGA CCTCAAACCT 
TCCTCCCCCA CAGACCAGCT TGTCACTCCC AAGGTCATAG CTCAGGGTGG TCCAAGCAGG 
ACAGGAAAGG CTTCCACAGC AAGTGCTGTG CTGGTTCCTA CTACCCCTGA ATTCCAGTCT 
CCAGTCCCGT CAGAACAGCC TCTTCCCCCT GACCCCATCC CTGAAGTCAA CTGCAGCAGG 
AGGCTGAGGG CCACTAGGAA ACATGGGTCT CCCACAGCTC ATGTTCATGA GCCCTGCACC 
GTACCCCCTG AACCTAACTC CCACTCTTCA AGGAACCAAA GACATGGGGC AGTGAAAGCA 
GCCAAGCCCC TTAGCACCAT TCCTGAGCCT GCCTTTGCCC AGCTCCCCGA GGCACCGCCT 
CACACTCCCC AGGTGCCAAA GGAGGAGGCA GCAGATGGAT CAGGCTTCAC CCCAGAGCCC 
CAGCCTAGGG CCTCTCAAAA CCACAAGAGG CCTTCAGCTA CTGCACATTC ACCTCCACTT 
CAAAAACGGC TCCAGAGAGG GAGAGTCCCT CAGAAGGCAG CATCCCTTAA GGAAGAAGAA 
AATCCAGTAG CGAGGCCGAG GAAGGAAGAG GGTGTAGTGA TTCCAGGTCC AGGCAAGAGA 
AAGAGAGAGC AGACAGAGGA GGAGTCCCAG GGAAGACCGA GCCGCAGCCT GCGACGGACC 
AAACCGATGC AGGAGTCCAC GGCCCCCAAA GTGCTGTTCA CCGGTGTGGT GGATGCTCGC 
GGAGAGAGGA CGGTGCTGGC CCTGGGGGGC AGTTTGGCCA GCTCGGTGGC CGAGGCTTCT 
CACCTGGTGA CTGATCGGAT CCGCCGGACG GTCAAGTTTC TGTGTGCCCT GGGCCGGGGC 
ATCCCCATCC TCTCCCTGGC CTGGCTGCAT GAGTCCCGCA AGGCAGGCTG CTTCTTGCCC 
CCGGACGAAT ATTTGGTGAC TGATCCTGAG CAGGAGAAGA ACTTCGGCTT CAGCCTTCGG 
GAGGCCCTGA GCCGAGCTCA GGAGCGAAGG CTGCTGGAGG GCTATGAGAT TCACGTGACC 
CCCGGAGTCC AGCCACCGCC ACCTCAGATG GGAGAAATCA TCAGCTGCTG TGGAGGCGCC 
ATCCTGCCCA GCATGCCCCG GTCCTACAAG CCTCAGAGGT TCGTGATCAC ATGTTCCCAG 
GACTTTCCTC GATGTGCTGT TCCATATCGG GTTGGGCTGC CTATCCTCTC ACCCGAATTC 
CTGCTGACGG GAGTACTGAA GCAGGAAGTC AAGCCAGAGG CCTTTGCCTT CTCCACTGTG 
GAAATGTCAT CCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MDC1 ( XM_012100992.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_011956382.1 Ovis aries mediator of DNA damage checkpoint 1 (MDC1), transcript variant X8, mRNA.

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CloneID	OSm28032
Clone ID Related Accession (Same CDS sequence)	XM_012100992.2
Accession Version	XM_012100992.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4968bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries(sheep)
Product	mediator of DNA damage checkpoint protein 1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014639029.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012100992.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGTGT CCCACTTTCC TTGTCTACAT TTTCCCTTTA AGATCATGGA AGACACCCAG 
ATTTTAAACT GGGAAGTTGA AGAAGAGGAG GAGGTTGCAG AGAGCCCCAA TGAATCTCTG 
GGGTATAGCT TGGAGCCCCT AGCACAGCTG CATATCTTCA GTAGTTCCTA TGGACCAGAA 
AAAGACTTTC CACTCTACCT TGGAAAGAAC ATGATAGGCC GAATGCCTGA TTGCTCTGTG 
GCCCTGCCCT TTTCATCAAT ATCCAAACAA CATGCAGTGA TTGAAATCTC TGCCTGGGAC 
AAGGCGCCTG TCCTTCGGGA TTGCGGGAGC CTCAATGGCA CTCAAATCCT GCGGCCTCCT 
AAGGTCCTGA GCCCTGGGGT GAGTCATCGT TTGCGGGACC GGGAGTTGAT TCTCTTTGCT 
GACTTGCCCT GCCAGTACCA TCGCTTGGAT GTCCCCCGGC CTTTTGTCTC CCGGGGCCCT 
CTAAGTGTAG AGGAGACACC CAGGGTACAG GGAGGAACTC AACCCTCCAG GCTTCTGTTG 
GCTGAGGACT CAGAGGAAGA AGTAGATTCC CTTTTGGACA AGTGTGTGGT GAAAGGACCA 
AGGACCTCCT CTTCGGCAAC AGTCGTTCCA GAGAGTGATG AAGAAGGGCC TTCCCCTGCC 
CCAGATGGGC CCGGGCCACC TTCTGCCTTC AACTTGAACA GCGACACGGA TGAGGAAGAA 
AGTCAGGAAT CAGGAGCAGG GGAGGCCTCT TCAGCTGCCA GAAGAGGTAC CGCTGCAGAG 
ACGGAACAGC CTGAACCTGT CACAGCCGAG ATCCAGATTG AGAAGGATCA GTGTTCCGTG 
AAGGAGAGGA ACGGGGACAC AGAAATCGAG AGGGATGTGA GGAATGGGGT GGTTCCGACT 
GGAGTGATTC TGGAGAGGAG CCAGCCTTCT GGGGAGGACA GTGACACAGA TGTGAGTGAT 
GAGAGTGGGC CTCGAAGAAG GCTGGTTGGA GTCCGTCCGA AAAGGGCCTG GTCTTGTAAC 
TTCATAGACA GTGATACCGA TGGAGAGGAC GAGGGGATCC CTGCTACCCC AGCAGTGGTT 
CCCATGAAGG AGAGGCAGAT CTTCCACGAA GCTGGTACAC AGAGCCCCCA GGCACCTGGT 
GTGGCACGTT GGCAGGAGAG CCCAGCTGAT GGTGATACAG ATATAGAGGA GGGGGAGGCC 
CCCCCAGACA GAAGCCAAGC CTCCGTGGTG ATCGACAGCA ATACAGACGA TGAGGAAGAA 
GTCTCAGCAG CACTGACACT GGCATGTCTA AGAGAGAGCC AGGCTGATTC TGAAGATCTG 
GACCTACCAG CTACCCAGTG CTTTGTAGAC AGAAAGAATC AGAGCCTGGA AGGTTCCTTG 
GATGAGTCAT GGGAGGCCTT GGCGACACAG CCATTCTGTC CGAGAGAATC TGAGGCCTCC 
GAGACCCAAA CCGTTGTCAC CCTCCTTGAC ACCCATGCAT CTTGGCCCTC TCCATCTAGG 
ACAGCACAGC AAGAGCAACA TCCAGAGAGC CCAGTCCATG CAGAGCCACT GGGGATGGAA 
GGCAGAGAGA TGCAGACTGT GGAGAAAGAA ATGGGTACCC CAAGAGAAAC AGCAGAGAGG 
GTGATCCCTG AGGGAGGGCC ACCGCAGGAG GAAACCAAGA AACTGCCCTC AGAAGGAGAG 
AGGGAAGATG TGACGGGAGA GGAAGAATTA ATCCGGGGGA TACAGGGCAG TGAAAAAAAT 
CAGGTGTTTG CTAGAGATAC TCAGAGCCAA GAATCTGACA AAAAAGTGAA AAGTGCAAGT 
ACTGGAAGGG GAATGGAGAT TTTAAAGGTA GAAATTGAGA CACCCAAGGA AACACAAGAG 
AAAGAGAGAG AAAAGCAGAC TCTCGCAGGG GAAATATTTG AGAGTGAAGC AGGGAAACTG 
GTAGTAGAGA GAGAGAGTGA GGCAGATGGG TTAGAAGTCA AGGAACCCCA AGAGCTACTG 
GACAGAAGCC CACAGATGGG GGAGACAGAG GCGGGGGACC AGGACCAGAA AGGCCAAGCC 
TCTGGTCTGC CATCAGAGCC TGGAGCAGGG GCAGGAGACC TTCAGGGACT TGCTTCAGAC 
CCAGTAGCTT CTGGGAGCCA GGCAGGTGGA GGAAGGGGAG CCCCAGGGAG CCCCAGGAGG 
CAGCAGAGAG GTGACTTGAA TTGCGAGATG CCACCTGCTC AGAAGGCTTC CAGGGGTGAT 
CAGGAATCCC CAGATGCTTG TCTGCCTCCT GCAGCGCCTG AAGCCTCAGC CGCACTCCCA 
AATTCCCTCA TCATCTCTCA AATCCAAAAA CATCCCGCAC CTCAGTCCCT CCTTTTTCCC 
TCTCTAGCTC CTTTAGAACT GCCTATTCCC AGGACCAGAC AAAATGAGAG TCAGGAAGCT 
CCAGAGACTC CCTTCTCCTC AGAGCTGAAC TCTGTCCACC CAGAACCCAA AGTCAGGCTC 
CAGGCGTCCT CTCCAGTTTC TTCTCTACCC CTTGAGCCCC ACCCTACCAC CCCCACAGGC 
CAGCCTATTG CCCTTGAACC CATATCTGGG GTCTCTCGGA GCAGGACACG TAGTTCCTTT 
GATGTAACTG CCTCATCAGT TGTCCCCACA GCCGTTGCAC TGCAGCCATC CACCTCCACA 
GACAAGCCTG TCACCCCTAA GCCCACACTT CGGGCCCCTC GGGGCAGGGC ACAGAGGTCT 
TCTGTCAAAA CCCCTGAACC CAATGTCACC ACAGACCAGC CTGTTGCCCC TGAGCTCACA 
GCTATGGCAA CTCGGGGCAG GGCACAGAGG TCTTCTGTCA AGATTCCCAA ACCAGATAAC 
CCCACAACAC CCAAGCCCCA GCCTTCCACT TCCACAGACC AGCCTGTCAT CCCCAAACCC 
ACATCTCGGG CCCCTCGGGG CAGGACACCT AGGTCTTCTG CCAAGACTCC TGAACCAGCT 
GTCCCCACAG CCTCTGAGCT CCAGCCTGCT GCCCCTAAAG ACCAGCCTGT TGCTCCTGAG 
CTCACATCTA GAGCCACTCG GGGCAAGACA CAGAGGTCTA TCAAGACTTC CAAACCAGAT 
ATGCCCACAA CTCCCGAGCC CCAGCCTTCC ACCTCCACAG ACCAGCCTGT CACCCCCAAA 
CCCACGTCTC GGGCCCCTCG GGGCAGGACA CCTAGGTCTT CCGCCAAGAC TCCTGAGCCA 
GTTGTCTCCA CAGCCTCTGA GCTCCAGCCT TCTGCCCTCA CAGATGAGCC TGTCACTCCT 
GAGCTCACAT CTAGGGCTAC TCGGGGTCGG GCACAGAGGT CCTCTGTCAA AACCCCTGAT 
CCAGTTACCA CCACAACACC TGAGCTCCAG CCTTCCACCT CCACAGACCA GCTTGTCACC 
CCCAAACGCA CATCTCGGGC CCCTCGAGGC AGGACACGTA GGTTGTCTGC CAAGACTCCT 
GAACCAGTTG TTCCCACAGC CTCTGAGCTC CAGCCTTCTG CCCCTGCAGA CCAGCCTGTT 
GGTCCTTGGG CCACTCAGTG TAGAAGACAT AGGTCTTCTG TCAAGACCCC AGAACCAGTT 
GTCCCCACAG TCCCTGAACC TCAGCCTTCC ACTTCTAAAG ACCAGTCTGT CACTCCTGAG 
CCCACATCTC AGGCCACTGA GAGCCAAACA CATAGGTCCT CTATCAAGAC ATCCCAGCCA 
ACGGAACCCA CAGCCTCTGA CCTCAAACCT TCCTCCCCCA CAGACCAGCT TGTCACTCCC 
AAGGTCATAG CTCAGGGTGG TCCAAGCAGG ACAGGAAAGG CTTCCACAGC AAGTGCTGTG 
CTGGTTCCTA CTACCCCTGA ATTCCAGTCT CCAGTCCCGT CAGAACAGCC TCTTCCCCCT 
GACCCCATCC CTGAAGTCAA CTGCAGCAGG AGGCTGAGGG CCACTAGGAA ACATGGGTCT 
CCCACAGCTC ATGTTCATGA GCCCTGCACC GTACCCCCTG AACCTAACTC CCACTCTTCA 
AGGAACCAAA GACATGGGGC AGTGAAAGCA GCCAAGCCCC TTAGCACCAT TCCTGAGCCT 
GCCTTTGCCC AGCTCCCCGA GGCACCGCCT CACACTCCCC AGGTGCCAAA GGAGGAGGCA 
GCAGATGGAT CAGGCTTCAC CCCAGAGCCC CAGCCTAGGG CCTCTCAAAA CCACAAGAGG 
CCTTCAGCTA CTGCACATTC ACCTCCACTT CAAAAACGGC TCCAGAGAGG GAGAGTCCCT 
CAGAAGGCAG CATCCCTTAA GGAAGAAGAA AATCCAGTAG CGAGGCCGAG GAAGGAAGAG 
GGTGTAGTGA TTCCAGGTCC AGGCAAGAGA AAGAGAGAGC AGACAGAGGA GGAGTCCCAG 
GGAAGACCGA GCCGCAGCCT GCGACGGACC AAACCGATGC AGGAGTCCAC GGCCCCCAAA 
GTGCTGTTCA CCGGTGTGGT GGATGCTCGC GGAGAGAGGA CGGTGCTGGC CCTGGGGGGC 
AGTTTGGCCA GCTCGGTGGC CGAGGCTTCT CACCTGGTGA CTGATCGGAT CCGCCGGACG 
GTCAAGTTTC TGTGTGCCCT GGGCCGGGGC ATCCCCATCC TCTCCCTGGC CTGGCTGCAT 
GAGTCCCGCA AGGCAGGCTG CTTCTTGCCC CCGGACGAAT ATTTGGTGAC TGATCCTGAG 
CAGGAGAAGA ACTTCGGCTT CAGCCTTCGG GAGGCCCTGA GCCGAGCTCA GGAGCGAAGG 
CTGCTGGAGG GCTATGAGAT TCACGTGACC CCCGGAGTCC AGCCACCGCC ACCTCAGATG 
GGAGAAATCA TCAGCTGCTG TGGAGGCGCC ATCCTGCCCA GCATGCCCCG GTCCTACAAG 
CCTCAGAGGT TCGTGATCAC ATGTTCCCAG GACTTTCCTC GATGTGCTGT TCCATATCGG 
GTTGGGCTGC CTATCCTCTC ACCCGAATTC CTGCTGACGG GAGTACTGAA GCAGGAAGTC 
AAGCCAGAGG CCTTTGCCTT CTCCACTGTG GAAATGTCAT CCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011956382.1
CDS	144..5111
Translation	MEVSHFPCLHFPFKIMEDTQILNWEVEEEEEVAESPNESLGYSLEPLAQLHIFSSSYGPEKDFPLYLGKNMIGRMPDCSVALPFSSISKQHAVIEISAWDKAPVLRDCGSLNGTQILRPPKVLSPGVSHRLRDRELILFADLPCQYHRLDVPRPFVSRGPLSVEETPRVQGGTQPSRLLLAEDSEEEVDSLLDKCVVKGPRTSSSATVVPESDEEGPSPAPDGPGPPSAFNLNSDTDEEESQESGAGEASSAARRGTAAETEQPEPVTAEIQIEKDQCSVKERNGDTEIERDVRNGVVPTGVILERSQPSGEDSDTDVSDESGPRRRLVGVRPKRAWSCNFIDSDTDGEDEGIPATPAVVPMKERQIFHEAGTQSPQAPGVARWQESPADGDTDIEEGEAPPDRSQASVVIDSNTDDEEEVSAALTLACLRESQADSEDLDLPATQCFVDRKNQSLEGSLDESWEALATQPFCPRESEASETQTVVTLLDTHASWPSPSRTAQQEQHPESPVHAEPLGMEGREMQTVEKEMGTPRETAERVIPEGGPPQEETKKLPSEGEREDVTGEEELIRGIQGSEKNQVFARDTQSQESDKKVKSASTGRGMEILKVEIETPKETQEKEREKQTLAGEIFESEAGKLVVERESEADGLEVKEPQELLDRSPQMGETEAGDQDQKGQASGLPSEPGAGAGDLQGLASDPVASGSQAGGGRGAPGSPRRQQRGDLNCEMPPAQKASRGDQESPDACLPPAAPEASAALPNSLIISQIQKHPAPQSLLFPSLAPLELPIPRTRQNESQEAPETPFSSELNSVHPEPKVRLQASSPVSSLPLEPHPTTPTGQPIALEPISGVSRSRTRSSFDVTASSVVPTAVALQPSTSTDKPVTPKPTLRAPRGRAQRSSVKTPEPNVTTDQPVAPELTAMATRGRAQRSSVKIPKPDNPTTPKPQPSTSTDQPVIPKPTSRAPRGRTPRSSAKTPEPAVPTASELQPAAPKDQPVAPELTSRATRGKTQRSIKTSKPDMPTTPEPQPSTSTDQPVTPKPTSRAPRGRTPRSSAKTPEPVVSTASELQPSALTDEPVTPELTSRATRGRAQRSSVKTPDPVTTTTPELQPSTSTDQLVTPKRTSRAPRGRTRRLSAKTPEPVVPTASELQPSAPADQPVGPWATQCRRHRSSVKTPEPVVPTVPEPQPSTSKDQSVTPEPTSQATESQTHRSSIKTSQPTEPTASDLKPSSPTDQLVTPKVIAQGGPSRTGKASTASAVLVPTTPEFQSPVPSEQPLPPDPIPEVNCSRRLRATRKHGSPTAHVHEPCTVPPEPNSHSSRNQRHGAVKAAKPLSTIPEPAFAQLPEAPPHTPQVPKEEAADGSGFTPEPQPRASQNHKRPSATAHSPPLQKRLQRGRVPQKAASLKEEENPVARPRKEEGVVIPGPGKRKREQTEEESQGRPSRSLRRTKPMQESTAPKVLFTGVVDARGERTVLALGGSLASSVAEASHLVTDRIRRTVKFLCALGRGIPILSLAWLHESRKAGCFLPPDEYLVTDPEQEKNFGFSLREALSRAQERRLLEGYEIHVTPGVQPPPPQMGEIISCCGGAILPSMPRSYKPQRFVITCSQDFPRCAVPYRVGLPILSPEFLLTGVLKQEVKPEAFAFSTVEMSST

Target ORF information:

RefSeq Version	XM_012100992.2
Organism	Ovis aries(sheep)
Definition	Ovis aries mediator of DNA damage checkpoint 1 (MDC1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012100992.2

ORF Insert Sequence:

ATGGAGGTGT CCCACTTTCC TTGTCTACAT TTTCCCTTTA AGATCATGGA AGACACCCAG 
ATTTTAAACT GGGAAGTTGA AGAAGAGGAG GAGGTTGCAG AGAGCCCCAA TGAATCTCTG 
GGGTATAGCT TGGAGCCCCT AGCACAGCTG CATATCTTCA GTAGTTCCTA TGGACCAGAA 
AAAGACTTTC CACTCTACCT TGGAAAGAAC ATGATAGGCC GAATGCCTGA TTGCTCTGTG 
GCCCTGCCCT TTTCATCAAT ATCCAAACAA CATGCAGTGA TTGAAATCTC TGCCTGGGAC 
AAGGCGCCTG TCCTTCGGGA TTGCGGGAGC CTCAATGGCA CTCAAATCCT GCGGCCTCCT 
AAGGTCCTGA GCCCTGGGGT GAGTCATCGT TTGCGGGACC GGGAGTTGAT TCTCTTTGCT 
GACTTGCCCT GCCAGTACCA TCGCTTGGAT GTCCCCCGGC CTTTTGTCTC CCGGGGCCCT 
CTAAGTGTAG AGGAGACACC CAGGGTACAG GGAGGAACTC AACCCTCCAG GCTTCTGTTG 
GCTGAGGACT CAGAGGAAGA AGTAGATTCC CTTTTGGACA AGTGTGTGGT GAAAGGACCA 
AGGACCTCCT CTTCGGCAAC AGTCGTTCCA GAGAGTGATG AAGAAGGGCC TTCCCCTGCC 
CCAGATGGGC CCGGGCCACC TTCTGCCTTC AACTTGAACA GCGACACGGA TGAGGAAGAA 
AGTCAGGAAT CAGGAGCAGG GGAGGCCTCT TCAGCTGCCA GAAGAGGTAC CGCTGCAGAG 
ACGGAACAGC CTGAACCTGT CACAGCCGAG ATCCAGATTG AGAAGGATCA GTGTTCCGTG 
AAGGAGAGGA ACGGGGACAC AGAAATCGAG AGGGATGTGA GGAATGGGGT GGTTCCGACT 
GGAGTGATTC TGGAGAGGAG CCAGCCTTCT GGGGAGGACA GTGACACAGA TGTGAGTGAT 
GAGAGTGGGC CTCGAAGAAG GCTGGTTGGA GTCCGTCCGA AAAGGGCCTG GTCTTGTAAC 
TTCATAGACA GTGATACCGA TGGAGAGGAC GAGGGGATCC CTGCTACCCC AGCAGTGGTT 
CCCATGAAGG AGAGGCAGAT CTTCCACGAA GCTGGTACAC AGAGCCCCCA GGCACCTGGT 
GTGGCACGTT GGCAGGAGAG CCCAGCTGAT GGTGATACAG ATATAGAGGA GGGGGAGGCC 
CCCCCAGACA GAAGCCAAGC CTCCGTGGTG ATCGACAGCA ATACAGACGA TGAGGAAGAA 
GTCTCAGCAG CACTGACACT GGCATGTCTA AGAGAGAGCC AGGCTGATTC TGAAGATCTG 
GACCTACCAG CTACCCAGTG CTTTGTAGAC AGAAAGAATC AGAGCCTGGA AGGTTCCTTG 
GATGAGTCAT GGGAGGCCTT GGCGACACAG CCATTCTGTC CGAGAGAATC TGAGGCCTCC 
GAGACCCAAA CCGTTGTCAC CCTCCTTGAC ACCCATGCAT CTTGGCCCTC TCCATCTAGG 
ACAGCACAGC AAGAGCAACA TCCAGAGAGC CCAGTCCATG CAGAGCCACT GGGGATGGAA 
GGCAGAGAGA TGCAGACTGT GGAGAAAGAA ATGGGTACCC CAAGAGAAAC AGCAGAGAGG 
GTGATCCCTG AGGGAGGGCC ACCGCAGGAG GAAACCAAGA AACTGCCCTC AGAAGGAGAG 
AGGGAAGATG TGACGGGAGA GGAAGAATTA ATCCGGGGGA TACAGGGCAG TGAAAAAAAT 
CAGGTGTTTG CTAGAGATAC TCAGAGCCAA GAATCTGACA AAAAAGTGAA AAGTGCAAGT 
ACTGGAAGGG GAATGGAGAT TTTAAAGGTA GAAATTGAGA CACCCAAGGA AACACAAGAG 
AAAGAGAGAG AAAAGCAGAC TCTCGCAGGG GAAATATTTG AGAGTGAAGC AGGGAAACTG 
GTAGTAGAGA GAGAGAGTGA GGCAGATGGG TTAGAAGTCA AGGAACCCCA AGAGCTACTG 
GACAGAAGCC CACAGATGGG GGAGACAGAG GCGGGGGACC AGGACCAGAA AGGCCAAGCC 
TCTGGTCTGC CATCAGAGCC TGGAGCAGGG GCAGGAGACC TTCAGGGACT TGCTTCAGAC 
CCAGTAGCTT CTGGGAGCCA GGCAGGTGGA GGAAGGGGAG CCCCAGGGAG CCCCAGGAGG 
CAGCAGAGAG GTGACTTGAA TTGCGAGATG CCACCTGCTC AGAAGGCTTC CAGGGGTGAT 
CAGGAATCCC CAGATGCTTG TCTGCCTCCT GCAGCGCCTG AAGCCTCAGC CGCACTCCCA 
AATTCCCTCA TCATCTCTCA AATCCAAAAA CATCCCGCAC CTCAGTCCCT CCTTTTTCCC 
TCTCTAGCTC CTTTAGAACT GCCTATTCCC AGGACCAGAC AAAATGAGAG TCAGGAAGCT 
CCAGAGACTC CCTTCTCCTC AGAGCTGAAC TCTGTCCACC CAGAACCCAA AGTCAGGCTC 
CAGGCGTCCT CTCCAGTTTC TTCTCTACCC CTTGAGCCCC ACCCTACCAC CCCCACAGGC 
CAGCCTATTG CCCTTGAACC CATATCTGGG GTCTCTCGGA GCAGGACACG TAGTTCCTTT 
GATGTAACTG CCTCATCAGT TGTCCCCACA GCCGTTGCAC TGCAGCCATC CACCTCCACA 
GACAAGCCTG TCACCCCTAA GCCCACACTT CGGGCCCCTC GGGGCAGGGC ACAGAGGTCT 
TCTGTCAAAA CCCCTGAACC CAATGTCACC ACAGACCAGC CTGTTGCCCC TGAGCTCACA 
GCTATGGCAA CTCGGGGCAG GGCACAGAGG TCTTCTGTCA AGATTCCCAA ACCAGATAAC 
CCCACAACAC CCAAGCCCCA GCCTTCCACT TCCACAGACC AGCCTGTCAT CCCCAAACCC 
ACATCTCGGG CCCCTCGGGG CAGGACACCT AGGTCTTCTG CCAAGACTCC TGAACCAGCT 
GTCCCCACAG CCTCTGAGCT CCAGCCTGCT GCCCCTAAAG ACCAGCCTGT TGCTCCTGAG 
CTCACATCTA GAGCCACTCG GGGCAAGACA CAGAGGTCTA TCAAGACTTC CAAACCAGAT 
ATGCCCACAA CTCCCGAGCC CCAGCCTTCC ACCTCCACAG ACCAGCCTGT CACCCCCAAA 
CCCACGTCTC GGGCCCCTCG GGGCAGGACA CCTAGGTCTT CCGCCAAGAC TCCTGAGCCA 
GTTGTCTCCA CAGCCTCTGA GCTCCAGCCT TCTGCCCTCA CAGATGAGCC TGTCACTCCT 
GAGCTCACAT CTAGGGCTAC TCGGGGTCGG GCACAGAGGT CCTCTGTCAA AACCCCTGAT 
CCAGTTACCA CCACAACACC TGAGCTCCAG CCTTCCACCT CCACAGACCA GCTTGTCACC 
CCCAAACGCA CATCTCGGGC CCCTCGAGGC AGGACACGTA GGTTGTCTGC CAAGACTCCT 
GAACCAGTTG TTCCCACAGC CTCTGAGCTC CAGCCTTCTG CCCCTGCAGA CCAGCCTGTT 
GGTCCTTGGG CCACTCAGTG TAGAAGACAT AGGTCTTCTG TCAAGACCCC AGAACCAGTT 
GTCCCCACAG TCCCTGAACC TCAGCCTTCC ACTTCTAAAG ACCAGTCTGT CACTCCTGAG 
CCCACATCTC AGGCCACTGA GAGCCAAACA CATAGGTCCT CTATCAAGAC ATCCCAGCCA 
ACGGAACCCA CAGCCTCTGA CCTCAAACCT TCCTCCCCCA CAGACCAGCT TGTCACTCCC 
AAGGTCATAG CTCAGGGTGG TCCAAGCAGG ACAGGAAAGG CTTCCACAGC AAGTGCTGTG 
CTGGTTCCTA CTACCCCTGA ATTCCAGTCT CCAGTCCCGT CAGAACAGCC TCTTCCCCCT 
GACCCCATCC CTGAAGTCAA CTGCAGCAGG AGGCTGAGGG CCACTAGGAA ACATGGGTCT 
CCCACAGCTC ATGTTCATGA GCCCTGCACC GTACCCCCTG AACCTAACTC CCACTCTTCA 
AGGAACCAAA GACATGGGGC AGTGAAAGCA GCCAAGCCCC TTAGCACCAT TCCTGAGCCT 
GCCTTTGCCC AGCTCCCCGA GGCACCGCCT CACACTCCCC AGGTGCCAAA GGAGGAGGCA 
GCAGATGGAT CAGGCTTCAC CCCAGAGCCC CAGCCTAGGG CCTCTCAAAA CCACAAGAGG 
CCTTCAGCTA CTGCACATTC ACCTCCACTT CAAAAACGGC TCCAGAGAGG GAGAGTCCCT 
CAGAAGGCAG CATCCCTTAA GGAAGAAGAA AATCCAGTAG CGAGGCCGAG GAAGGAAGAG 
GGTGTAGTGA TTCCAGGTCC AGGCAAGAGA AAGAGAGAGC AGACAGAGGA GGAGTCCCAG 
GGAAGACCGA GCCGCAGCCT GCGACGGACC AAACCGATGC AGGAGTCCAC GGCCCCCAAA 
GTGCTGTTCA CCGGTGTGGT GGATGCTCGC GGAGAGAGGA CGGTGCTGGC CCTGGGGGGC 
AGTTTGGCCA GCTCGGTGGC CGAGGCTTCT CACCTGGTGA CTGATCGGAT CCGCCGGACG 
GTCAAGTTTC TGTGTGCCCT GGGCCGGGGC ATCCCCATCC TCTCCCTGGC CTGGCTGCAT 
GAGTCCCGCA AGGCAGGCTG CTTCTTGCCC CCGGACGAAT ATTTGGTGAC TGATCCTGAG 
CAGGAGAAGA ACTTCGGCTT CAGCCTTCGG GAGGCCCTGA GCCGAGCTCA GGAGCGAAGG 
CTGCTGGAGG GCTATGAGAT TCACGTGACC CCCGGAGTCC AGCCACCGCC ACCTCAGATG 
GGAGAAATCA TCAGCTGCTG TGGAGGCGCC ATCCTGCCCA GCATGCCCCG GTCCTACAAG 
CCTCAGAGGT TCGTGATCAC ATGTTCCCAG GACTTTCCTC GATGTGCTGT TCCATATCGG 
GTTGGGCTGC CTATCCTCTC ACCCGAATTC CTGCTGACGG GAGTACTGAA GCAGGAAGTC 
AAGCCAGAGG CCTTTGCCTT CTCCACTGTG GAAATGTCAT CCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	MDC1
Entrez Gene ID	101107143
Full Name	mediator of DNA damage checkpoint 1
Gene Type	protein-coding
Organism	Ovis aries(sheep)

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

MDC1 cDNA ORF clone, Ovis aries(sheep)

Gene

Clones

gRNAs

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