MAN2A2 gene cDNA ORF clone, Ovis aries(sheep)

mRNA	Protein	Name
XM_012098482.2	XP_011953872.1	alpha-mannosidase 2x isoform X1
XM_012098485.2	XP_011953875.1	alpha-mannosidase 2x isoform X4
XM_012133339.2	XP_011988729.1	alpha-mannosidase 2x isoform X2
XM_012133341.2	XP_011988731.1	alpha-mannosidase 2x isoform X4
XM_015101896.1	XP_014957382.1	alpha-mannosidase 2x isoform X1
XM_012133336.2	XP_011988726.1	alpha-mannosidase 2x isoform X1
XM_012098484.2	XP_011953874.1	alpha-mannosidase 2x isoform X3
XM_027957080.1	XP_027812881.1	alpha-mannosidase 2x isoform X2
XM_027957081.1	XP_027812882.1	alpha-mannosidase 2x isoform X3
XM_012098481.2	XP_011953871.1	alpha-mannosidase 2x isoform X1
XM_012133338.1	XP_011988728.1	alpha-mannosidase 2x isoform X1
XM_012133340.2	XP_011988730.1	alpha-mannosidase 2x isoform X3
XM_015101896.2	XP_014957382.2	alpha-mannosidase 2x isoform X1
XM_027957079.1	XP_027812880.1	alpha-mannosidase 2x isoform X1
XM_012133337.2	XP_011988727.1	alpha-mannosidase 2x isoform X1
XM_012098483.1	XP_011953873.1	alpha-mannosidase 2x isoform X1
XM_004017759.3	XP_004017808.1	alpha-mannosidase 2x isoform X2

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OOv15680	XM_012133336.2 Latest version!	Ovis aries musimon mannosidase, alpha, class 2A, member 2 (MAN2A2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OOv15680	XM_012133337.2 Latest version!	Ovis aries musimon mannosidase, alpha, class 2A, member 2 (MAN2A2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OOv15680	XM_012133338.1 Latest version!	Ovis aries musimon mannosidase, alpha, class 2A, member 2 (MAN2A2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OOv15681	XM_012133339.2 Latest version!	Ovis aries musimon mannosidase, alpha, class 2A, member 2 (MAN2A2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OOv15682	XM_012133340.2 Latest version!	Ovis aries musimon mannosidase, alpha, class 2A, member 2 (MAN2A2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OOv15683	XM_012133341.2 Latest version!	Ovis aries musimon mannosidase, alpha, class 2A, member 2 (MAN2A2), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OSm67701	XM_027957079.1 Latest version!	Ovis aries mannosidase alpha class 2A member 2 (MAN2A2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OSm67701	XM_015101896.2 Latest version!	Ovis aries mannosidase alpha class 2A member 2 (MAN2A2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OSm67702	XM_027957080.1 Latest version!	Ovis aries mannosidase alpha class 2A member 2 (MAN2A2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OSm67703	XM_027957081.1 Latest version!	Ovis aries mannosidase alpha class 2A member 2 (MAN2A2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OSm14138	XM_004017759.3 Latest version!	Ovis aries mannosidase, alpha, class 2A, member 2 (MAN2A2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OSm27643	XM_012098483.1 Latest version!	Ovis aries mannosidase, alpha, class 2A, member 2 (MAN2A2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OSm27643	XM_012098482.2 Latest version!	Ovis aries mannosidase, alpha, class 2A, member 2 (MAN2A2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OSm27643	XM_012098481.2 Latest version!	Ovis aries mannosidase, alpha, class 2A, member 2 (MAN2A2), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OSm27643	XM_015101896.1 Latest version!	Ovis aries mannosidase, alpha, class 2A, member 2 (MAN2A2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OSm27644	XM_012098484.2 Latest version!	Ovis aries mannosidase, alpha, class 2A, member 2 (MAN2A2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OSm27645	XM_012098485.2 Latest version!	Ovis aries mannosidase, alpha, class 2A, member 2 (MAN2A2), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

MAN2A2 ( XM_012133336.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_011988726.1 Ovis aries musimon mannosidase, alpha, class 2A, member 2 (MAN2A2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OOv15680
Clone ID Related Accession (Same CDS sequence)	XM_012133336.2 , XM_012133338.1 , XM_012133337.2
Accession Version	XM_012133336.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3528bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	alpha-mannosidase 2x isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942386.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012133336.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCTGA AAAAGCAGGT GACAGTGTGT GGGGCTGCTA TCTTCTGCGT GGCTGTCTTC 
TCGCTCTACC TCATGTTGGA CCGAGTACAG CATGATCCTG CTCGACACCA GAACGGTGGG 
AACTTCCCCC GGAGTCAGAT ATCTGTGCTA CAGAACCGCA TTGAACAGCT GGAACAGCTG 
TTGGAGGAGA ACCATGAGAT CATCAGCCAC ATCAAAGACT CTGTGCTGGA GCTGACAGCC 
AACGTGGAGG GCCCACCCGC CCTGCTGCCC TACTATGTGG CCAACGGCTC CTGGGTGGTG 
CCGCCAGAGC CCCGGCCCAG CTTCTTCTCC ATCTCCCCTC AAGACTGCCA GTTTGCTTTG 
GGGGGCCGCG GCGAGAAGCC AGAGCTGCAG ATGCTGACTG TATCAGAGGA GTTACCGTTT 
GACAACGTGG ATGGCGGCGT GTGGAAGCAA GGCTTCGACA TTTCCTACAG CCCGCACGAC 
TGGGACACCG AAGACCTGCA GGTGTTTGTG GTCCCCCACT CTCACAATGA CCCCGGCTGG 
CTCAAGACCT TTGACAAGTA CTACACAGAA CAGACCCAGC ACATCCTCAA CAACATGGTG 
TCCAAGCTGC AGGAGGACCC CCGGCGGCGC TTCATCTGGG CAGAAGTCTC CTTCTTTGCC 
AAGTGGTGGG ACAACATCAG TGCCCAAAAG AGAGCAGCAG TCCGAAGGCT GGTGGGAAAC 
GGGCAGCTGG AGATTGCAAC AGGAGGCTGG GTGATGCCGG ACGAGGCCAA CTCCCACTAT 
TTTGCATTGA TTGACCAGCT CATTGAGGGC CACCAGTGGC TGGAGAAGAA CCTGGGTGCA 
ACTCCGCGCT CGGGCTGGGC GGTGGACCCC TTTGGATACA GCCCCACCAT GCCTTACCTG 
CTGCGCCGTG CCAACCTGAC CAGCATGCTC ATTCAGAGGG TACACTACGC CATCAAGAAG 
CACTTTGCTG CCAGCCACAG CCTGGAGTTC ATGTGGAGGC AGACCTGGGA CTCGGATGCC 
AGCACAGACA TCTTCTGCCA CATGATGCCT TTCTACAGCT ACGATGTCCC CCATACGTGT 
GGCCCGGATC CCAAGATCTG CTGCCAGTTC GATTTCAAGC GCTTGCCTGG CGGGCGTATC 
AGCTGCCCTT GGAAGGTGCC GCCCCGGGCT ATCACGGAGG CCAATGTGGC TGAGAGGGCA 
GGCCTGCTCC TGGACCAGTA CAGGAAGAAG TCCCGGCTCT TCCGGAGCAA TGTGCTCCTG 
GTGCCGCTGG GCGATGACTT CCGCTACGAC AAGCCCCAGG AGTGGGACGC CCAGTTCTTC 
AACTACCAGC GGCTCTTCGA CTTCCTCAAC AGCAAGCCTG ACCTCCATGT GCAGGCCCAG 
TTTGGCACCC TCTCCGACTA TTTTGACGCT CTATACAAGA GGACAGGGGT GGAGCCGGGA 
GCCCGGCCTC CAGGTTTCCC TGTGCTGAGT GGGGATTTCT TCTCCTACGC GGACCGGGAG 
GACCACTACT GGACAGGCTA TTACACTTCA CGGCCTTTCT ACAAGAACCT GGACCGCGTC 
CTGGAAGCAC ACCTGCGGGG TGCAGAGATT CTGTACAGCC TGGCCGTGGC TCACGCCCGC 
CGCGCTGGAC TGGCCAGCCA GTTCCCACTC TCAAACTTCG CCCTTCTGAC GGACGCTCGG 
CGCACACTGG GGCTCTTCCA GCACCATGAC GCAATCACGG GCACCGCCAA GGAGGCCGTT 
GTGGTGGACT ATGGGGTCAG GCTTCTGCGC TCCCTTGTCA GCCTGAAACA GGTCATCATG 
AACGCAGCTC ACTACCTGGT GCTGGGGGAC AAGAAGGCCT ACCACTTTGA CCCAGAGGTG 
CCCTTCCTGC AGATGGATGA CACCCGCTTA AATCACGACG CCCTTCCAGA GCGCACAGTG 
ATCCAGCTGG AATCCTCACC CAGGTATGTG GTGCTGTTCA ACCCACTGGA GCAGGAGCGG 
CTCAGCGTGG TGTCCCTGCT GGTGAGCTCA CCCCGGGTGC GCGTGCTTTC GGAGGAGGGT 
CAGCCCCTGG CCGTGCAGGT CAGCGCGCAC TGGAGCTCTG CCACCGACAT GGTCCCTGAC 
GTCTATCAGG TGTCTGTGCC TGTCCGCCTG CCAGCGCTGG GTCTTGGCGT GCTGCAGCTG 
CAGCAGGGCC TGGATGGGCC CCGCACGCTG CCCTCCTCTG TGCGCGTCTA CCTACATGGG 
CGGCCGCTGT CCGTCAGCAG GAACGAGGCG TTCCCTCTCC GTGTCATTGA CTCTGGCACC 
AGTGACTTCG CCCTCAGCAA CCGCTACATG CAGGTCTGGT TCTCAGGCCT TACCGGGCTC 
CTCAAGAGCG TCCGAAGGGT GGATGAGGAG CAGGAACAGC GGGTGGACAT GGAGTTCCTC 
GTCTATGGCA CCCGCTCGTC CAAAGACAAG AGTGGAGCCT ACCTCTTCCT GCCTGACGGC 
GAGGCCAAGC CCTACGTCCC CAGGGACCCA CCCGTGCTGC GCGTCACAGA AGGCCCTTTC 
TTCTCAGAGG TGGTTGCCTG CTACGAGCAT GTTCACCAGG TGGTCCGGCT GTATAACCTG 
CCGGGGGTGG AGGGGCTGTC CCTGGATGTG TCGTCCTTGG TGGACATCCG GGACTACGTC 
AACAAGGAGC TGGCCCTGCG CATTCGCACT GACATCGATA GCCAGGGCAT CTTCTTCACA 
GACCTCAACG GCTTTCAGGT GCAGCCCCGA CGGTATCTGA AGAAGCTGCC CCTGCAGGCC 
AACTTCTACC CCATGCCCGT CATGGCCTAC CTCCAGGACG CTCAGAACCG CCTCACGCTG 
CACACAGCCC AGGCCCTGGG TGTCTCCAGC CTCCACAATG GCCAGCTAGA GGTGATCTTG 
GACCGGAGAC TGATGCAGGA CGACAACCGG GGCCTTGGCC AAGGGCTCAA GGACAACAAG 
AGAACCTGCA ACCACTTCCG CCTCCTGTTG GAACGGCGAA CCCTGGGCAG GGAGCCTGGC 
TTTTTCTCCA AACTGGCAGC CATGTTTAGG GGCTTGATCT TTCACAGCAG CAGGAACAGG 
AACCGAGAGG TCCGCGAGGG CTCCTCTGCA AGCTTCCCGT CCCTCCTCAG CCACCTGACC 
TCCATGTACC TGAACACCCC TGTGCTCGCC CTGCCTGTGG CCAGGAGGCA GGCCCCCGGC 
CCCGCTCTGC GCTCTTTTCA CCCTCTGGCC TCCTCACTGC CCTGTGACTT CCACCTGCTC 
AACCTGCGGA TGCTCCAGGC CGAGGAGGAT GGCTTGCCCT CGGCAGAAGC TGCGCTCCTC 
TTACACCGCA AGGGTTTTGA CTGTGGCCTT GAGGCCAAGA ACTTGGGCTT CAACTGCACC 
ACCAGCCAAG GCAAGGTGGC CCTGGGCAGC CTCTTCCATG GCCTGGACGT GGTTTTCCTG 
CAGCCAACCT CCTTGACCCT GCTGTACCCT CTGGCCTCGC CCTCCAATAG CACTGACGTC 
TATGTGGAGC CCATGGAGAT CGCCACCTTC CGCCTCCGCT TGGGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011988726.1
CDS	434..3961
Translation	MKLKKQVTVCGAAIFCVAVFSLYLMLDRVQHDPARHQNGGNFPRSQISVLQNRIEQLEQLLEENHEIISHIKDSVLELTANVEGPPALLPYYVANGSWVVPPEPRPSFFSISPQDCQFALGGRGEKPELQMLTVSEELPFDNVDGGVWKQGFDISYSPHDWDTEDLQVFVVPHSHNDPGWLKTFDKYYTEQTQHILNNMVSKLQEDPRRRFIWAEVSFFAKWWDNISAQKRAAVRRLVGNGQLEIATGGWVMPDEANSHYFALIDQLIEGHQWLEKNLGATPRSGWAVDPFGYSPTMPYLLRRANLTSMLIQRVHYAIKKHFAASHSLEFMWRQTWDSDASTDIFCHMMPFYSYDVPHTCGPDPKICCQFDFKRLPGGRISCPWKVPPRAITEANVAERAGLLLDQYRKKSRLFRSNVLLVPLGDDFRYDKPQEWDAQFFNYQRLFDFLNSKPDLHVQAQFGTLSDYFDALYKRTGVEPGARPPGFPVLSGDFFSYADREDHYWTGYYTSRPFYKNLDRVLEAHLRGAEILYSLAVAHARRAGLASQFPLSNFALLTDARRTLGLFQHHDAITGTAKEAVVVDYGVRLLRSLVSLKQVIMNAAHYLVLGDKKAYHFDPEVPFLQMDDTRLNHDALPERTVIQLESSPRYVVLFNPLEQERLSVVSLLVSSPRVRVLSEEGQPLAVQVSAHWSSATDMVPDVYQVSVPVRLPALGLGVLQLQQGLDGPRTLPSSVRVYLHGRPLSVSRNEAFPLRVIDSGTSDFALSNRYMQVWFSGLTGLLKSVRRVDEEQEQRVDMEFLVYGTRSSKDKSGAYLFLPDGEAKPYVPRDPPVLRVTEGPFFSEVVACYEHVHQVVRLYNLPGVEGLSLDVSSLVDIRDYVNKELALRIRTDIDSQGIFFTDLNGFQVQPRRYLKKLPLQANFYPMPVMAYLQDAQNRLTLHTAQALGVSSLHNGQLEVILDRRLMQDDNRGLGQGLKDNKRTCNHFRLLLERRTLGREPGFFSKLAAMFRGLIFHSSRNRNREVREGSSASFPSLLSHLTSMYLNTPVLALPVARRQAPGPALRSFHPLASSLPCDFHLLNLRMLQAEEDGLPSAEAALLLHRKGFDCGLEAKNLGFNCTTSQGKVALGSLFHGLDVVFLQPTSLTLLYPLASPSNSTDVYVEPMEIATFRLRLG

Target ORF information:

RefSeq Version	XM_012133336.2
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon mannosidase, alpha, class 2A, member 2 (MAN2A2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012133336.2

ORF Insert Sequence:

ATGAAGCTGA AAAAGCAGGT GACAGTGTGT GGGGCTGCTA TCTTCTGCGT GGCTGTCTTC 
TCGCTCTACC TCATGTTGGA CCGAGTACAG CATGATCCTG CTCGACACCA GAACGGTGGG 
AACTTCCCCC GGAGTCAGAT ATCTGTGCTA CAGAACCGCA TTGAACAGCT GGAACAGCTG 
TTGGAGGAGA ACCATGAGAT CATCAGCCAC ATCAAAGACT CTGTGCTGGA GCTGACAGCC 
AACGTGGAGG GCCCACCCGC CCTGCTGCCC TACTATGTGG CCAACGGCTC CTGGGTGGTG 
CCGCCAGAGC CCCGGCCCAG CTTCTTCTCC ATCTCCCCTC AAGACTGCCA GTTTGCTTTG 
GGGGGCCGCG GCGAGAAGCC AGAGCTGCAG ATGCTGACTG TATCAGAGGA GTTACCGTTT 
GACAACGTGG ATGGCGGCGT GTGGAAGCAA GGCTTCGACA TTTCCTACAG CCCGCACGAC 
TGGGACACCG AAGACCTGCA GGTGTTTGTG GTCCCCCACT CTCACAATGA CCCCGGCTGG 
CTCAAGACCT TTGACAAGTA CTACACAGAA CAGACCCAGC ACATCCTCAA CAACATGGTG 
TCCAAGCTGC AGGAGGACCC CCGGCGGCGC TTCATCTGGG CAGAAGTCTC CTTCTTTGCC 
AAGTGGTGGG ACAACATCAG TGCCCAAAAG AGAGCAGCAG TCCGAAGGCT GGTGGGAAAC 
GGGCAGCTGG AGATTGCAAC AGGAGGCTGG GTGATGCCGG ACGAGGCCAA CTCCCACTAT 
TTTGCATTGA TTGACCAGCT CATTGAGGGC CACCAGTGGC TGGAGAAGAA CCTGGGTGCA 
ACTCCGCGCT CGGGCTGGGC GGTGGACCCC TTTGGATACA GCCCCACCAT GCCTTACCTG 
CTGCGCCGTG CCAACCTGAC CAGCATGCTC ATTCAGAGGG TACACTACGC CATCAAGAAG 
CACTTTGCTG CCAGCCACAG CCTGGAGTTC ATGTGGAGGC AGACCTGGGA CTCGGATGCC 
AGCACAGACA TCTTCTGCCA CATGATGCCT TTCTACAGCT ACGATGTCCC CCATACGTGT 
GGCCCGGATC CCAAGATCTG CTGCCAGTTC GATTTCAAGC GCTTGCCTGG CGGGCGTATC 
AGCTGCCCTT GGAAGGTGCC GCCCCGGGCT ATCACGGAGG CCAATGTGGC TGAGAGGGCA 
GGCCTGCTCC TGGACCAGTA CAGGAAGAAG TCCCGGCTCT TCCGGAGCAA TGTGCTCCTG 
GTGCCGCTGG GCGATGACTT CCGCTACGAC AAGCCCCAGG AGTGGGACGC CCAGTTCTTC 
AACTACCAGC GGCTCTTCGA CTTCCTCAAC AGCAAGCCTG ACCTCCATGT GCAGGCCCAG 
TTTGGCACCC TCTCCGACTA TTTTGACGCT CTATACAAGA GGACAGGGGT GGAGCCGGGA 
GCCCGGCCTC CAGGTTTCCC TGTGCTGAGT GGGGATTTCT TCTCCTACGC GGACCGGGAG 
GACCACTACT GGACAGGCTA TTACACTTCA CGGCCTTTCT ACAAGAACCT GGACCGCGTC 
CTGGAAGCAC ACCTGCGGGG TGCAGAGATT CTGTACAGCC TGGCCGTGGC TCACGCCCGC 
CGCGCTGGAC TGGCCAGCCA GTTCCCACTC TCAAACTTCG CCCTTCTGAC GGACGCTCGG 
CGCACACTGG GGCTCTTCCA GCACCATGAC GCAATCACGG GCACCGCCAA GGAGGCCGTT 
GTGGTGGACT ATGGGGTCAG GCTTCTGCGC TCCCTTGTCA GCCTGAAACA GGTCATCATG 
AACGCAGCTC ACTACCTGGT GCTGGGGGAC AAGAAGGCCT ACCACTTTGA CCCAGAGGTG 
CCCTTCCTGC AGATGGATGA CACCCGCTTA AATCACGACG CCCTTCCAGA GCGCACAGTG 
ATCCAGCTGG AATCCTCACC CAGGTATGTG GTGCTGTTCA ACCCACTGGA GCAGGAGCGG 
CTCAGCGTGG TGTCCCTGCT GGTGAGCTCA CCCCGGGTGC GCGTGCTTTC GGAGGAGGGT 
CAGCCCCTGG CCGTGCAGGT CAGCGCGCAC TGGAGCTCTG CCACCGACAT GGTCCCTGAC 
GTCTATCAGG TGTCTGTGCC TGTCCGCCTG CCAGCGCTGG GTCTTGGCGT GCTGCAGCTG 
CAGCAGGGCC TGGATGGGCC CCGCACGCTG CCCTCCTCTG TGCGCGTCTA CCTACATGGG 
CGGCCGCTGT CCGTCAGCAG GAACGAGGCG TTCCCTCTCC GTGTCATTGA CTCTGGCACC 
AGTGACTTCG CCCTCAGCAA CCGCTACATG CAGGTCTGGT TCTCAGGCCT TACCGGGCTC 
CTCAAGAGCG TCCGAAGGGT GGATGAGGAG CAGGAACAGC GGGTGGACAT GGAGTTCCTC 
GTCTATGGCA CCCGCTCGTC CAAAGACAAG AGTGGAGCCT ACCTCTTCCT GCCTGACGGC 
GAGGCCAAGC CCTACGTCCC CAGGGACCCA CCCGTGCTGC GCGTCACAGA AGGCCCTTTC 
TTCTCAGAGG TGGTTGCCTG CTACGAGCAT GTTCACCAGG TGGTCCGGCT GTATAACCTG 
CCGGGGGTGG AGGGGCTGTC CCTGGATGTG TCGTCCTTGG TGGACATCCG GGACTACGTC 
AACAAGGAGC TGGCCCTGCG CATTCGCACT GACATCGATA GCCAGGGCAT CTTCTTCACA 
GACCTCAACG GCTTTCAGGT GCAGCCCCGA CGGTATCTGA AGAAGCTGCC CCTGCAGGCC 
AACTTCTACC CCATGCCCGT CATGGCCTAC CTCCAGGACG CTCAGAACCG CCTCACGCTG 
CACACAGCCC AGGCCCTGGG TGTCTCCAGC CTCCACAATG GCCAGCTAGA GGTGATCTTG 
GACCGGAGAC TGATGCAGGA CGACAACCGG GGCCTTGGCC AAGGGCTCAA GGACAACAAG 
AGAACCTGCA ACCACTTCCG CCTCCTGTTG GAACGGCGAA CCCTGGGCAG GGAGCCTGGC 
TTTTTCTCCA AACTGGCAGC CATGTTTAGG GGCTTGATCT TTCACAGCAG CAGGAACAGG 
AACCGAGAGG TCCGCGAGGG CTCCTCTGCA AGCTTCCCGT CCCTCCTCAG CCACCTGACC 
TCCATGTACC TGAACACCCC TGTGCTCGCC CTGCCTGTGG CCAGGAGGCA GGCCCCCGGC 
CCCGCTCTGC GCTCTTTTCA CCCTCTGGCC TCCTCACTGC CCTGTGACTT CCACCTGCTC 
AACCTGCGGA TGCTCCAGGC CGAGGAGGAT GGCTTGCCCT CGGCAGAAGC TGCGCTCCTC 
TTACACCGCA AGGGTTTTGA CTGTGGCCTT GAGGCCAAGA ACTTGGGCTT CAACTGCACC 
ACCAGCCAAG GCAAGGTGGC CCTGGGCAGC CTCTTCCATG GCCTGGACGT GGTTTTCCTG 
CAGCCAACCT CCTTGACCCT GCTGTACCCT CTGGCCTCGC CCTCCAATAG CACTGACGTC 
TATGTGGAGC CCATGGAGAT CGCCACCTTC CGCCTCCGCT TGGGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAN2A2 ( XM_012133338.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_011988728.1 Ovis aries musimon mannosidase, alpha, class 2A, member 2 (MAN2A2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OOv15680
Clone ID Related Accession (Same CDS sequence)	XM_012133336.2 , XM_012133338.1 , XM_012133337.2
Accession Version	XM_012133338.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3528bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	alpha-mannosidase 2x isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942386.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCTGA AAAAGCAGGT GACAGTGTGT GGGGCTGCTA TCTTCTGCGT GGCTGTCTTC 
TCGCTCTACC TCATGTTGGA CCGAGTACAG CATGATCCTG CTCGACACCA GAACGGTGGG 
AACTTCCCCC GGAGTCAGAT ATCTGTGCTA CAGAACCGCA TTGAACAGCT GGAACAGCTG 
TTGGAGGAGA ACCATGAGAT CATCAGCCAC ATCAAAGACT CTGTGCTGGA GCTGACAGCC 
AACGTGGAGG GCCCACCCGC CCTGCTGCCC TACTATGTGG CCAACGGCTC CTGGGTGGTG 
CCGCCAGAGC CCCGGCCCAG CTTCTTCTCC ATCTCCCCTC AAGACTGCCA GTTTGCTTTG 
GGGGGCCGCG GCGAGAAGCC AGAGCTGCAG ATGCTGACTG TATCAGAGGA GTTACCGTTT 
GACAACGTGG ATGGCGGCGT GTGGAAGCAA GGCTTCGACA TTTCCTACAG CCCGCACGAC 
TGGGACACCG AAGACCTGCA GGTGTTTGTG GTCCCCCACT CTCACAATGA CCCCGGCTGG 
CTCAAGACCT TTGACAAGTA CTACACAGAA CAGACCCAGC ACATCCTCAA CAACATGGTG 
TCCAAGCTGC AGGAGGACCC CCGGCGGCGC TTCATCTGGG CAGAAGTCTC CTTCTTTGCC 
AAGTGGTGGG ACAACATCAG TGCCCAAAAG AGAGCAGCAG TCCGAAGGCT GGTGGGAAAC 
GGGCAGCTGG AGATTGCAAC AGGAGGCTGG GTGATGCCGG ACGAGGCCAA CTCCCACTAT 
TTTGCATTGA TTGACCAGCT CATTGAGGGC CACCAGTGGC TGGAGAAGAA CCTGGGTGCA 
ACTCCGCGCT CGGGCTGGGC GGTGGACCCC TTTGGATACA GCCCCACCAT GCCTTACCTG 
CTGCGCCGTG CCAACCTGAC CAGCATGCTC ATTCAGAGGG TACACTACGC CATCAAGAAG 
CACTTTGCTG CCAGCCACAG CCTGGAGTTC ATGTGGAGGC AGACCTGGGA CTCGGATGCC 
AGCACAGACA TCTTCTGCCA CATGATGCCT TTCTACAGCT ACGATGTCCC CCATACGTGT 
GGCCCGGATC CCAAGATCTG CTGCCAGTTC GATTTCAAGC GCTTGCCTGG CGGGCGTATC 
AGCTGCCCTT GGAAGGTGCC GCCCCGGGCT ATCACGGAGG CCAATGTGGC TGAGAGGGCA 
GGCCTGCTCC TGGACCAGTA CAGGAAGAAG TCCCGGCTCT TCCGGAGCAA TGTGCTCCTG 
GTGCCGCTGG GCGATGACTT CCGCTACGAC AAGCCCCAGG AGTGGGACGC CCAGTTCTTC 
AACTACCAGC GGCTCTTCGA CTTCCTCAAC AGCAAGCCTG ACCTCCATGT GCAGGCCCAG 
TTTGGCACCC TCTCCGACTA TTTTGACGCT CTATACAAGA GGACAGGGGT GGAGCCGGGA 
GCCCGGCCTC CAGGTTTCCC TGTGCTGAGT GGGGATTTCT TCTCCTACGC GGACCGGGAG 
GACCACTACT GGACAGGCTA TTACACTTCA CGGCCTTTCT ACAAGAACCT GGACCGCGTC 
CTGGAAGCAC ACCTGCGGGG TGCAGAGATT CTGTACAGCC TGGCCGTGGC TCACGCCCGC 
CGCGCTGGAC TGGCCAGCCA GTTCCCACTC TCAAACTTCG CCCTTCTGAC GGACGCTCGG 
CGCACACTGG GGCTCTTCCA GCACCATGAC GCAATCACGG GCACCGCCAA GGAGGCCGTT 
GTGGTGGACT ATGGGGTCAG GCTTCTGCGC TCCCTTGTCA GCCTGAAACA GGTCATCATG 
AACGCAGCTC ACTACCTGGT GCTGGGGGAC AAGAAGGCCT ACCACTTTGA CCCAGAGGTG 
CCCTTCCTGC AGATGGATGA CACCCGCTTA AATCACGACG CCCTTCCAGA GCGCACAGTG 
ATCCAGCTGG AATCCTCACC CAGGTATGTG GTGCTGTTCA ACCCACTGGA GCAGGAGCGG 
CTCAGCGTGG TGTCCCTGCT GGTGAGCTCA CCCCGGGTGC GCGTGCTTTC GGAGGAGGGT 
CAGCCCCTGG CCGTGCAGGT CAGCGCGCAC TGGAGCTCTG CCACCGACAT GGTCCCTGAC 
GTCTATCAGG TGTCTGTGCC TGTCCGCCTG CCAGCGCTGG GTCTTGGCGT GCTGCAGCTG 
CAGCAGGGCC TGGATGGGCC CCGCACGCTG CCCTCCTCTG TGCGCGTCTA CCTACATGGG 
CGGCCGCTGT CCGTCAGCAG GAACGAGGCG TTCCCTCTCC GTGTCATTGA CTCTGGCACC 
AGTGACTTCG CCCTCAGCAA CCGCTACATG CAGGTCTGGT TCTCAGGCCT TACCGGGCTC 
CTCAAGAGCG TCCGAAGGGT GGATGAGGAG CAGGAACAGC GGGTGGACAT GGAGTTCCTC 
GTCTATGGCA CCCGCTCGTC CAAAGACAAG AGTGGAGCCT ACCTCTTCCT GCCTGACGGC 
GAGGCCAAGC CCTACGTCCC CAGGGACCCA CCCGTGCTGC GCGTCACAGA AGGCCCTTTC 
TTCTCAGAGG TGGTTGCCTG CTACGAGCAT GTTCACCAGG TGGTCCGGCT GTATAACCTG 
CCGGGGGTGG AGGGGCTGTC CCTGGATGTG TCGTCCTTGG TGGACATCCG GGACTACGTC 
AACAAGGAGC TGGCCCTGCG CATTCGCACT GACATCGATA GCCAGGGCAT CTTCTTCACA 
GACCTCAACG GCTTTCAGGT GCAGCCCCGA CGGTATCTGA AGAAGCTGCC CCTGCAGGCC 
AACTTCTACC CCATGCCCGT CATGGCCTAC CTCCAGGACG CTCAGAACCG CCTCACGCTG 
CACACAGCCC AGGCCCTGGG TGTCTCCAGC CTCCACAATG GCCAGCTAGA GGTGATCTTG 
GACCGGAGAC TGATGCAGGA CGACAACCGG GGCCTTGGCC AAGGGCTCAA GGACAACAAG 
AGAACCTGCA ACCACTTCCG CCTCCTGTTG GAACGGCGAA CCCTGGGCAG GGAGCCTGGC 
TTTTTCTCCA AACTGGCAGC CATGTTTAGG GGCTTGATCT TTCACAGCAG CAGGAACAGG 
AACCGAGAGG TCCGCGAGGG CTCCTCTGCA AGCTTCCCGT CCCTCCTCAG CCACCTGACC 
TCCATGTACC TGAACACCCC TGTGCTCGCC CTGCCTGTGG CCAGGAGGCA GGCCCCCGGC 
CCCGCTCTGC GCTCTTTTCA CCCTCTGGCC TCCTCACTGC CCTGTGACTT CCACCTGCTC 
AACCTGCGGA TGCTCCAGGC CGAGGAGGAT GGCTTGCCCT CGGCAGAAGC TGCGCTCCTC 
TTACACCGCA AGGGTTTTGA CTGTGGCCTT GAGGCCAAGA ACTTGGGCTT CAACTGCACC 
ACCAGCCAAG GCAAGGTGGC CCTGGGCAGC CTCTTCCATG GCCTGGACGT GGTTTTCCTG 
CAGCCAACCT CCTTGACCCT GCTGTACCCT CTGGCCTCGC CCTCCAATAG CACTGACGTC 
TATGTGGAGC CCATGGAGAT CGCCACCTTC CGCCTCCGCT TGGGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011988728.1
CDS	312..3839
Translation	MKLKKQVTVCGAAIFCVAVFSLYLMLDRVQHDPARHQNGGNFPRSQISVLQNRIEQLEQLLEENHEIISHIKDSVLELTANVEGPPALLPYYVANGSWVVPPEPRPSFFSISPQDCQFALGGRGEKPELQMLTVSEELPFDNVDGGVWKQGFDISYSPHDWDTEDLQVFVVPHSHNDPGWLKTFDKYYTEQTQHILNNMVSKLQEDPRRRFIWAEVSFFAKWWDNISAQKRAAVRRLVGNGQLEIATGGWVMPDEANSHYFALIDQLIEGHQWLEKNLGATPRSGWAVDPFGYSPTMPYLLRRANLTSMLIQRVHYAIKKHFAASHSLEFMWRQTWDSDASTDIFCHMMPFYSYDVPHTCGPDPKICCQFDFKRLPGGRISCPWKVPPRAITEANVAERAGLLLDQYRKKSRLFRSNVLLVPLGDDFRYDKPQEWDAQFFNYQRLFDFLNSKPDLHVQAQFGTLSDYFDALYKRTGVEPGARPPGFPVLSGDFFSYADREDHYWTGYYTSRPFYKNLDRVLEAHLRGAEILYSLAVAHARRAGLASQFPLSNFALLTDARRTLGLFQHHDAITGTAKEAVVVDYGVRLLRSLVSLKQVIMNAAHYLVLGDKKAYHFDPEVPFLQMDDTRLNHDALPERTVIQLESSPRYVVLFNPLEQERLSVVSLLVSSPRVRVLSEEGQPLAVQVSAHWSSATDMVPDVYQVSVPVRLPALGLGVLQLQQGLDGPRTLPSSVRVYLHGRPLSVSRNEAFPLRVIDSGTSDFALSNRYMQVWFSGLTGLLKSVRRVDEEQEQRVDMEFLVYGTRSSKDKSGAYLFLPDGEAKPYVPRDPPVLRVTEGPFFSEVVACYEHVHQVVRLYNLPGVEGLSLDVSSLVDIRDYVNKELALRIRTDIDSQGIFFTDLNGFQVQPRRYLKKLPLQANFYPMPVMAYLQDAQNRLTLHTAQALGVSSLHNGQLEVILDRRLMQDDNRGLGQGLKDNKRTCNHFRLLLERRTLGREPGFFSKLAAMFRGLIFHSSRNRNREVREGSSASFPSLLSHLTSMYLNTPVLALPVARRQAPGPALRSFHPLASSLPCDFHLLNLRMLQAEEDGLPSAEAALLLHRKGFDCGLEAKNLGFNCTTSQGKVALGSLFHGLDVVFLQPTSLTLLYPLASPSNSTDVYVEPMEIATFRLRLG

Target ORF information:

RefSeq Version	XM_012133338.1
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon mannosidase, alpha, class 2A, member 2 (MAN2A2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012133338.1

ORF Insert Sequence:

ATGAAGCTGA AAAAGCAGGT GACAGTGTGT GGGGCTGCTA TCTTCTGCGT GGCTGTCTTC 
TCGCTCTACC TCATGTTGGA CCGAGTACAG CATGATCCTG CTCGACACCA GAACGGTGGG 
AACTTCCCCC GGAGTCAGAT ATCTGTGCTA CAGAACCGCA TTGAACAGCT GGAACAGCTG 
TTGGAGGAGA ACCATGAGAT CATCAGCCAC ATCAAAGACT CTGTGCTGGA GCTGACAGCC 
AACGTGGAGG GCCCACCCGC CCTGCTGCCC TACTATGTGG CCAACGGCTC CTGGGTGGTG 
CCGCCAGAGC CCCGGCCCAG CTTCTTCTCC ATCTCCCCTC AAGACTGCCA GTTTGCTTTG 
GGGGGCCGCG GCGAGAAGCC AGAGCTGCAG ATGCTGACTG TATCAGAGGA GTTACCGTTT 
GACAACGTGG ATGGCGGCGT GTGGAAGCAA GGCTTCGACA TTTCCTACAG CCCGCACGAC 
TGGGACACCG AAGACCTGCA GGTGTTTGTG GTCCCCCACT CTCACAATGA CCCCGGCTGG 
CTCAAGACCT TTGACAAGTA CTACACAGAA CAGACCCAGC ACATCCTCAA CAACATGGTG 
TCCAAGCTGC AGGAGGACCC CCGGCGGCGC TTCATCTGGG CAGAAGTCTC CTTCTTTGCC 
AAGTGGTGGG ACAACATCAG TGCCCAAAAG AGAGCAGCAG TCCGAAGGCT GGTGGGAAAC 
GGGCAGCTGG AGATTGCAAC AGGAGGCTGG GTGATGCCGG ACGAGGCCAA CTCCCACTAT 
TTTGCATTGA TTGACCAGCT CATTGAGGGC CACCAGTGGC TGGAGAAGAA CCTGGGTGCA 
ACTCCGCGCT CGGGCTGGGC GGTGGACCCC TTTGGATACA GCCCCACCAT GCCTTACCTG 
CTGCGCCGTG CCAACCTGAC CAGCATGCTC ATTCAGAGGG TACACTACGC CATCAAGAAG 
CACTTTGCTG CCAGCCACAG CCTGGAGTTC ATGTGGAGGC AGACCTGGGA CTCGGATGCC 
AGCACAGACA TCTTCTGCCA CATGATGCCT TTCTACAGCT ACGATGTCCC CCATACGTGT 
GGCCCGGATC CCAAGATCTG CTGCCAGTTC GATTTCAAGC GCTTGCCTGG CGGGCGTATC 
AGCTGCCCTT GGAAGGTGCC GCCCCGGGCT ATCACGGAGG CCAATGTGGC TGAGAGGGCA 
GGCCTGCTCC TGGACCAGTA CAGGAAGAAG TCCCGGCTCT TCCGGAGCAA TGTGCTCCTG 
GTGCCGCTGG GCGATGACTT CCGCTACGAC AAGCCCCAGG AGTGGGACGC CCAGTTCTTC 
AACTACCAGC GGCTCTTCGA CTTCCTCAAC AGCAAGCCTG ACCTCCATGT GCAGGCCCAG 
TTTGGCACCC TCTCCGACTA TTTTGACGCT CTATACAAGA GGACAGGGGT GGAGCCGGGA 
GCCCGGCCTC CAGGTTTCCC TGTGCTGAGT GGGGATTTCT TCTCCTACGC GGACCGGGAG 
GACCACTACT GGACAGGCTA TTACACTTCA CGGCCTTTCT ACAAGAACCT GGACCGCGTC 
CTGGAAGCAC ACCTGCGGGG TGCAGAGATT CTGTACAGCC TGGCCGTGGC TCACGCCCGC 
CGCGCTGGAC TGGCCAGCCA GTTCCCACTC TCAAACTTCG CCCTTCTGAC GGACGCTCGG 
CGCACACTGG GGCTCTTCCA GCACCATGAC GCAATCACGG GCACCGCCAA GGAGGCCGTT 
GTGGTGGACT ATGGGGTCAG GCTTCTGCGC TCCCTTGTCA GCCTGAAACA GGTCATCATG 
AACGCAGCTC ACTACCTGGT GCTGGGGGAC AAGAAGGCCT ACCACTTTGA CCCAGAGGTG 
CCCTTCCTGC AGATGGATGA CACCCGCTTA AATCACGACG CCCTTCCAGA GCGCACAGTG 
ATCCAGCTGG AATCCTCACC CAGGTATGTG GTGCTGTTCA ACCCACTGGA GCAGGAGCGG 
CTCAGCGTGG TGTCCCTGCT GGTGAGCTCA CCCCGGGTGC GCGTGCTTTC GGAGGAGGGT 
CAGCCCCTGG CCGTGCAGGT CAGCGCGCAC TGGAGCTCTG CCACCGACAT GGTCCCTGAC 
GTCTATCAGG TGTCTGTGCC TGTCCGCCTG CCAGCGCTGG GTCTTGGCGT GCTGCAGCTG 
CAGCAGGGCC TGGATGGGCC CCGCACGCTG CCCTCCTCTG TGCGCGTCTA CCTACATGGG 
CGGCCGCTGT CCGTCAGCAG GAACGAGGCG TTCCCTCTCC GTGTCATTGA CTCTGGCACC 
AGTGACTTCG CCCTCAGCAA CCGCTACATG CAGGTCTGGT TCTCAGGCCT TACCGGGCTC 
CTCAAGAGCG TCCGAAGGGT GGATGAGGAG CAGGAACAGC GGGTGGACAT GGAGTTCCTC 
GTCTATGGCA CCCGCTCGTC CAAAGACAAG AGTGGAGCCT ACCTCTTCCT GCCTGACGGC 
GAGGCCAAGC CCTACGTCCC CAGGGACCCA CCCGTGCTGC GCGTCACAGA AGGCCCTTTC 
TTCTCAGAGG TGGTTGCCTG CTACGAGCAT GTTCACCAGG TGGTCCGGCT GTATAACCTG 
CCGGGGGTGG AGGGGCTGTC CCTGGATGTG TCGTCCTTGG TGGACATCCG GGACTACGTC 
AACAAGGAGC TGGCCCTGCG CATTCGCACT GACATCGATA GCCAGGGCAT CTTCTTCACA 
GACCTCAACG GCTTTCAGGT GCAGCCCCGA CGGTATCTGA AGAAGCTGCC CCTGCAGGCC 
AACTTCTACC CCATGCCCGT CATGGCCTAC CTCCAGGACG CTCAGAACCG CCTCACGCTG 
CACACAGCCC AGGCCCTGGG TGTCTCCAGC CTCCACAATG GCCAGCTAGA GGTGATCTTG 
GACCGGAGAC TGATGCAGGA CGACAACCGG GGCCTTGGCC AAGGGCTCAA GGACAACAAG 
AGAACCTGCA ACCACTTCCG CCTCCTGTTG GAACGGCGAA CCCTGGGCAG GGAGCCTGGC 
TTTTTCTCCA AACTGGCAGC CATGTTTAGG GGCTTGATCT TTCACAGCAG CAGGAACAGG 
AACCGAGAGG TCCGCGAGGG CTCCTCTGCA AGCTTCCCGT CCCTCCTCAG CCACCTGACC 
TCCATGTACC TGAACACCCC TGTGCTCGCC CTGCCTGTGG CCAGGAGGCA GGCCCCCGGC 
CCCGCTCTGC GCTCTTTTCA CCCTCTGGCC TCCTCACTGC CCTGTGACTT CCACCTGCTC 
AACCTGCGGA TGCTCCAGGC CGAGGAGGAT GGCTTGCCCT CGGCAGAAGC TGCGCTCCTC 
TTACACCGCA AGGGTTTTGA CTGTGGCCTT GAGGCCAAGA ACTTGGGCTT CAACTGCACC 
ACCAGCCAAG GCAAGGTGGC CCTGGGCAGC CTCTTCCATG GCCTGGACGT GGTTTTCCTG 
CAGCCAACCT CCTTGACCCT GCTGTACCCT CTGGCCTCGC CCTCCAATAG CACTGACGTC 
TATGTGGAGC CCATGGAGAT CGCCACCTTC CGCCTCCGCT TGGGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAN2A2 ( XM_012133337.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_011988727.1 Ovis aries musimon mannosidase, alpha, class 2A, member 2 (MAN2A2), transcript variant X2, mRNA.

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CloneID	OOv15680
Clone ID Related Accession (Same CDS sequence)	XM_012133336.2 , XM_012133338.1 , XM_012133337.2
Accession Version	XM_012133337.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3528bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	alpha-mannosidase 2x isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942386.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012133337.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCTGA AAAAGCAGGT GACAGTGTGT GGGGCTGCTA TCTTCTGCGT GGCTGTCTTC 
TCGCTCTACC TCATGTTGGA CCGAGTACAG CATGATCCTG CTCGACACCA GAACGGTGGG 
AACTTCCCCC GGAGTCAGAT ATCTGTGCTA CAGAACCGCA TTGAACAGCT GGAACAGCTG 
TTGGAGGAGA ACCATGAGAT CATCAGCCAC ATCAAAGACT CTGTGCTGGA GCTGACAGCC 
AACGTGGAGG GCCCACCCGC CCTGCTGCCC TACTATGTGG CCAACGGCTC CTGGGTGGTG 
CCGCCAGAGC CCCGGCCCAG CTTCTTCTCC ATCTCCCCTC AAGACTGCCA GTTTGCTTTG 
GGGGGCCGCG GCGAGAAGCC AGAGCTGCAG ATGCTGACTG TATCAGAGGA GTTACCGTTT 
GACAACGTGG ATGGCGGCGT GTGGAAGCAA GGCTTCGACA TTTCCTACAG CCCGCACGAC 
TGGGACACCG AAGACCTGCA GGTGTTTGTG GTCCCCCACT CTCACAATGA CCCCGGCTGG 
CTCAAGACCT TTGACAAGTA CTACACAGAA CAGACCCAGC ACATCCTCAA CAACATGGTG 
TCCAAGCTGC AGGAGGACCC CCGGCGGCGC TTCATCTGGG CAGAAGTCTC CTTCTTTGCC 
AAGTGGTGGG ACAACATCAG TGCCCAAAAG AGAGCAGCAG TCCGAAGGCT GGTGGGAAAC 
GGGCAGCTGG AGATTGCAAC AGGAGGCTGG GTGATGCCGG ACGAGGCCAA CTCCCACTAT 
TTTGCATTGA TTGACCAGCT CATTGAGGGC CACCAGTGGC TGGAGAAGAA CCTGGGTGCA 
ACTCCGCGCT CGGGCTGGGC GGTGGACCCC TTTGGATACA GCCCCACCAT GCCTTACCTG 
CTGCGCCGTG CCAACCTGAC CAGCATGCTC ATTCAGAGGG TACACTACGC CATCAAGAAG 
CACTTTGCTG CCAGCCACAG CCTGGAGTTC ATGTGGAGGC AGACCTGGGA CTCGGATGCC 
AGCACAGACA TCTTCTGCCA CATGATGCCT TTCTACAGCT ACGATGTCCC CCATACGTGT 
GGCCCGGATC CCAAGATCTG CTGCCAGTTC GATTTCAAGC GCTTGCCTGG CGGGCGTATC 
AGCTGCCCTT GGAAGGTGCC GCCCCGGGCT ATCACGGAGG CCAATGTGGC TGAGAGGGCA 
GGCCTGCTCC TGGACCAGTA CAGGAAGAAG TCCCGGCTCT TCCGGAGCAA TGTGCTCCTG 
GTGCCGCTGG GCGATGACTT CCGCTACGAC AAGCCCCAGG AGTGGGACGC CCAGTTCTTC 
AACTACCAGC GGCTCTTCGA CTTCCTCAAC AGCAAGCCTG ACCTCCATGT GCAGGCCCAG 
TTTGGCACCC TCTCCGACTA TTTTGACGCT CTATACAAGA GGACAGGGGT GGAGCCGGGA 
GCCCGGCCTC CAGGTTTCCC TGTGCTGAGT GGGGATTTCT TCTCCTACGC GGACCGGGAG 
GACCACTACT GGACAGGCTA TTACACTTCA CGGCCTTTCT ACAAGAACCT GGACCGCGTC 
CTGGAAGCAC ACCTGCGGGG TGCAGAGATT CTGTACAGCC TGGCCGTGGC TCACGCCCGC 
CGCGCTGGAC TGGCCAGCCA GTTCCCACTC TCAAACTTCG CCCTTCTGAC GGACGCTCGG 
CGCACACTGG GGCTCTTCCA GCACCATGAC GCAATCACGG GCACCGCCAA GGAGGCCGTT 
GTGGTGGACT ATGGGGTCAG GCTTCTGCGC TCCCTTGTCA GCCTGAAACA GGTCATCATG 
AACGCAGCTC ACTACCTGGT GCTGGGGGAC AAGAAGGCCT ACCACTTTGA CCCAGAGGTG 
CCCTTCCTGC AGATGGATGA CACCCGCTTA AATCACGACG CCCTTCCAGA GCGCACAGTG 
ATCCAGCTGG AATCCTCACC CAGGTATGTG GTGCTGTTCA ACCCACTGGA GCAGGAGCGG 
CTCAGCGTGG TGTCCCTGCT GGTGAGCTCA CCCCGGGTGC GCGTGCTTTC GGAGGAGGGT 
CAGCCCCTGG CCGTGCAGGT CAGCGCGCAC TGGAGCTCTG CCACCGACAT GGTCCCTGAC 
GTCTATCAGG TGTCTGTGCC TGTCCGCCTG CCAGCGCTGG GTCTTGGCGT GCTGCAGCTG 
CAGCAGGGCC TGGATGGGCC CCGCACGCTG CCCTCCTCTG TGCGCGTCTA CCTACATGGG 
CGGCCGCTGT CCGTCAGCAG GAACGAGGCG TTCCCTCTCC GTGTCATTGA CTCTGGCACC 
AGTGACTTCG CCCTCAGCAA CCGCTACATG CAGGTCTGGT TCTCAGGCCT TACCGGGCTC 
CTCAAGAGCG TCCGAAGGGT GGATGAGGAG CAGGAACAGC GGGTGGACAT GGAGTTCCTC 
GTCTATGGCA CCCGCTCGTC CAAAGACAAG AGTGGAGCCT ACCTCTTCCT GCCTGACGGC 
GAGGCCAAGC CCTACGTCCC CAGGGACCCA CCCGTGCTGC GCGTCACAGA AGGCCCTTTC 
TTCTCAGAGG TGGTTGCCTG CTACGAGCAT GTTCACCAGG TGGTCCGGCT GTATAACCTG 
CCGGGGGTGG AGGGGCTGTC CCTGGATGTG TCGTCCTTGG TGGACATCCG GGACTACGTC 
AACAAGGAGC TGGCCCTGCG CATTCGCACT GACATCGATA GCCAGGGCAT CTTCTTCACA 
GACCTCAACG GCTTTCAGGT GCAGCCCCGA CGGTATCTGA AGAAGCTGCC CCTGCAGGCC 
AACTTCTACC CCATGCCCGT CATGGCCTAC CTCCAGGACG CTCAGAACCG CCTCACGCTG 
CACACAGCCC AGGCCCTGGG TGTCTCCAGC CTCCACAATG GCCAGCTAGA GGTGATCTTG 
GACCGGAGAC TGATGCAGGA CGACAACCGG GGCCTTGGCC AAGGGCTCAA GGACAACAAG 
AGAACCTGCA ACCACTTCCG CCTCCTGTTG GAACGGCGAA CCCTGGGCAG GGAGCCTGGC 
TTTTTCTCCA AACTGGCAGC CATGTTTAGG GGCTTGATCT TTCACAGCAG CAGGAACAGG 
AACCGAGAGG TCCGCGAGGG CTCCTCTGCA AGCTTCCCGT CCCTCCTCAG CCACCTGACC 
TCCATGTACC TGAACACCCC TGTGCTCGCC CTGCCTGTGG CCAGGAGGCA GGCCCCCGGC 
CCCGCTCTGC GCTCTTTTCA CCCTCTGGCC TCCTCACTGC CCTGTGACTT CCACCTGCTC 
AACCTGCGGA TGCTCCAGGC CGAGGAGGAT GGCTTGCCCT CGGCAGAAGC TGCGCTCCTC 
TTACACCGCA AGGGTTTTGA CTGTGGCCTT GAGGCCAAGA ACTTGGGCTT CAACTGCACC 
ACCAGCCAAG GCAAGGTGGC CCTGGGCAGC CTCTTCCATG GCCTGGACGT GGTTTTCCTG 
CAGCCAACCT CCTTGACCCT GCTGTACCCT CTGGCCTCGC CCTCCAATAG CACTGACGTC 
TATGTGGAGC CCATGGAGAT CGCCACCTTC CGCCTCCGCT TGGGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011988727.1
CDS	403..3930
Translation	MKLKKQVTVCGAAIFCVAVFSLYLMLDRVQHDPARHQNGGNFPRSQISVLQNRIEQLEQLLEENHEIISHIKDSVLELTANVEGPPALLPYYVANGSWVVPPEPRPSFFSISPQDCQFALGGRGEKPELQMLTVSEELPFDNVDGGVWKQGFDISYSPHDWDTEDLQVFVVPHSHNDPGWLKTFDKYYTEQTQHILNNMVSKLQEDPRRRFIWAEVSFFAKWWDNISAQKRAAVRRLVGNGQLEIATGGWVMPDEANSHYFALIDQLIEGHQWLEKNLGATPRSGWAVDPFGYSPTMPYLLRRANLTSMLIQRVHYAIKKHFAASHSLEFMWRQTWDSDASTDIFCHMMPFYSYDVPHTCGPDPKICCQFDFKRLPGGRISCPWKVPPRAITEANVAERAGLLLDQYRKKSRLFRSNVLLVPLGDDFRYDKPQEWDAQFFNYQRLFDFLNSKPDLHVQAQFGTLSDYFDALYKRTGVEPGARPPGFPVLSGDFFSYADREDHYWTGYYTSRPFYKNLDRVLEAHLRGAEILYSLAVAHARRAGLASQFPLSNFALLTDARRTLGLFQHHDAITGTAKEAVVVDYGVRLLRSLVSLKQVIMNAAHYLVLGDKKAYHFDPEVPFLQMDDTRLNHDALPERTVIQLESSPRYVVLFNPLEQERLSVVSLLVSSPRVRVLSEEGQPLAVQVSAHWSSATDMVPDVYQVSVPVRLPALGLGVLQLQQGLDGPRTLPSSVRVYLHGRPLSVSRNEAFPLRVIDSGTSDFALSNRYMQVWFSGLTGLLKSVRRVDEEQEQRVDMEFLVYGTRSSKDKSGAYLFLPDGEAKPYVPRDPPVLRVTEGPFFSEVVACYEHVHQVVRLYNLPGVEGLSLDVSSLVDIRDYVNKELALRIRTDIDSQGIFFTDLNGFQVQPRRYLKKLPLQANFYPMPVMAYLQDAQNRLTLHTAQALGVSSLHNGQLEVILDRRLMQDDNRGLGQGLKDNKRTCNHFRLLLERRTLGREPGFFSKLAAMFRGLIFHSSRNRNREVREGSSASFPSLLSHLTSMYLNTPVLALPVARRQAPGPALRSFHPLASSLPCDFHLLNLRMLQAEEDGLPSAEAALLLHRKGFDCGLEAKNLGFNCTTSQGKVALGSLFHGLDVVFLQPTSLTLLYPLASPSNSTDVYVEPMEIATFRLRLG

Target ORF information:

RefSeq Version	XM_012133337.2
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon mannosidase, alpha, class 2A, member 2 (MAN2A2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012133337.2

ORF Insert Sequence:

ATGAAGCTGA AAAAGCAGGT GACAGTGTGT GGGGCTGCTA TCTTCTGCGT GGCTGTCTTC 
TCGCTCTACC TCATGTTGGA CCGAGTACAG CATGATCCTG CTCGACACCA GAACGGTGGG 
AACTTCCCCC GGAGTCAGAT ATCTGTGCTA CAGAACCGCA TTGAACAGCT GGAACAGCTG 
TTGGAGGAGA ACCATGAGAT CATCAGCCAC ATCAAAGACT CTGTGCTGGA GCTGACAGCC 
AACGTGGAGG GCCCACCCGC CCTGCTGCCC TACTATGTGG CCAACGGCTC CTGGGTGGTG 
CCGCCAGAGC CCCGGCCCAG CTTCTTCTCC ATCTCCCCTC AAGACTGCCA GTTTGCTTTG 
GGGGGCCGCG GCGAGAAGCC AGAGCTGCAG ATGCTGACTG TATCAGAGGA GTTACCGTTT 
GACAACGTGG ATGGCGGCGT GTGGAAGCAA GGCTTCGACA TTTCCTACAG CCCGCACGAC 
TGGGACACCG AAGACCTGCA GGTGTTTGTG GTCCCCCACT CTCACAATGA CCCCGGCTGG 
CTCAAGACCT TTGACAAGTA CTACACAGAA CAGACCCAGC ACATCCTCAA CAACATGGTG 
TCCAAGCTGC AGGAGGACCC CCGGCGGCGC TTCATCTGGG CAGAAGTCTC CTTCTTTGCC 
AAGTGGTGGG ACAACATCAG TGCCCAAAAG AGAGCAGCAG TCCGAAGGCT GGTGGGAAAC 
GGGCAGCTGG AGATTGCAAC AGGAGGCTGG GTGATGCCGG ACGAGGCCAA CTCCCACTAT 
TTTGCATTGA TTGACCAGCT CATTGAGGGC CACCAGTGGC TGGAGAAGAA CCTGGGTGCA 
ACTCCGCGCT CGGGCTGGGC GGTGGACCCC TTTGGATACA GCCCCACCAT GCCTTACCTG 
CTGCGCCGTG CCAACCTGAC CAGCATGCTC ATTCAGAGGG TACACTACGC CATCAAGAAG 
CACTTTGCTG CCAGCCACAG CCTGGAGTTC ATGTGGAGGC AGACCTGGGA CTCGGATGCC 
AGCACAGACA TCTTCTGCCA CATGATGCCT TTCTACAGCT ACGATGTCCC CCATACGTGT 
GGCCCGGATC CCAAGATCTG CTGCCAGTTC GATTTCAAGC GCTTGCCTGG CGGGCGTATC 
AGCTGCCCTT GGAAGGTGCC GCCCCGGGCT ATCACGGAGG CCAATGTGGC TGAGAGGGCA 
GGCCTGCTCC TGGACCAGTA CAGGAAGAAG TCCCGGCTCT TCCGGAGCAA TGTGCTCCTG 
GTGCCGCTGG GCGATGACTT CCGCTACGAC AAGCCCCAGG AGTGGGACGC CCAGTTCTTC 
AACTACCAGC GGCTCTTCGA CTTCCTCAAC AGCAAGCCTG ACCTCCATGT GCAGGCCCAG 
TTTGGCACCC TCTCCGACTA TTTTGACGCT CTATACAAGA GGACAGGGGT GGAGCCGGGA 
GCCCGGCCTC CAGGTTTCCC TGTGCTGAGT GGGGATTTCT TCTCCTACGC GGACCGGGAG 
GACCACTACT GGACAGGCTA TTACACTTCA CGGCCTTTCT ACAAGAACCT GGACCGCGTC 
CTGGAAGCAC ACCTGCGGGG TGCAGAGATT CTGTACAGCC TGGCCGTGGC TCACGCCCGC 
CGCGCTGGAC TGGCCAGCCA GTTCCCACTC TCAAACTTCG CCCTTCTGAC GGACGCTCGG 
CGCACACTGG GGCTCTTCCA GCACCATGAC GCAATCACGG GCACCGCCAA GGAGGCCGTT 
GTGGTGGACT ATGGGGTCAG GCTTCTGCGC TCCCTTGTCA GCCTGAAACA GGTCATCATG 
AACGCAGCTC ACTACCTGGT GCTGGGGGAC AAGAAGGCCT ACCACTTTGA CCCAGAGGTG 
CCCTTCCTGC AGATGGATGA CACCCGCTTA AATCACGACG CCCTTCCAGA GCGCACAGTG 
ATCCAGCTGG AATCCTCACC CAGGTATGTG GTGCTGTTCA ACCCACTGGA GCAGGAGCGG 
CTCAGCGTGG TGTCCCTGCT GGTGAGCTCA CCCCGGGTGC GCGTGCTTTC GGAGGAGGGT 
CAGCCCCTGG CCGTGCAGGT CAGCGCGCAC TGGAGCTCTG CCACCGACAT GGTCCCTGAC 
GTCTATCAGG TGTCTGTGCC TGTCCGCCTG CCAGCGCTGG GTCTTGGCGT GCTGCAGCTG 
CAGCAGGGCC TGGATGGGCC CCGCACGCTG CCCTCCTCTG TGCGCGTCTA CCTACATGGG 
CGGCCGCTGT CCGTCAGCAG GAACGAGGCG TTCCCTCTCC GTGTCATTGA CTCTGGCACC 
AGTGACTTCG CCCTCAGCAA CCGCTACATG CAGGTCTGGT TCTCAGGCCT TACCGGGCTC 
CTCAAGAGCG TCCGAAGGGT GGATGAGGAG CAGGAACAGC GGGTGGACAT GGAGTTCCTC 
GTCTATGGCA CCCGCTCGTC CAAAGACAAG AGTGGAGCCT ACCTCTTCCT GCCTGACGGC 
GAGGCCAAGC CCTACGTCCC CAGGGACCCA CCCGTGCTGC GCGTCACAGA AGGCCCTTTC 
TTCTCAGAGG TGGTTGCCTG CTACGAGCAT GTTCACCAGG TGGTCCGGCT GTATAACCTG 
CCGGGGGTGG AGGGGCTGTC CCTGGATGTG TCGTCCTTGG TGGACATCCG GGACTACGTC 
AACAAGGAGC TGGCCCTGCG CATTCGCACT GACATCGATA GCCAGGGCAT CTTCTTCACA 
GACCTCAACG GCTTTCAGGT GCAGCCCCGA CGGTATCTGA AGAAGCTGCC CCTGCAGGCC 
AACTTCTACC CCATGCCCGT CATGGCCTAC CTCCAGGACG CTCAGAACCG CCTCACGCTG 
CACACAGCCC AGGCCCTGGG TGTCTCCAGC CTCCACAATG GCCAGCTAGA GGTGATCTTG 
GACCGGAGAC TGATGCAGGA CGACAACCGG GGCCTTGGCC AAGGGCTCAA GGACAACAAG 
AGAACCTGCA ACCACTTCCG CCTCCTGTTG GAACGGCGAA CCCTGGGCAG GGAGCCTGGC 
TTTTTCTCCA AACTGGCAGC CATGTTTAGG GGCTTGATCT TTCACAGCAG CAGGAACAGG 
AACCGAGAGG TCCGCGAGGG CTCCTCTGCA AGCTTCCCGT CCCTCCTCAG CCACCTGACC 
TCCATGTACC TGAACACCCC TGTGCTCGCC CTGCCTGTGG CCAGGAGGCA GGCCCCCGGC 
CCCGCTCTGC GCTCTTTTCA CCCTCTGGCC TCCTCACTGC CCTGTGACTT CCACCTGCTC 
AACCTGCGGA TGCTCCAGGC CGAGGAGGAT GGCTTGCCCT CGGCAGAAGC TGCGCTCCTC 
TTACACCGCA AGGGTTTTGA CTGTGGCCTT GAGGCCAAGA ACTTGGGCTT CAACTGCACC 
ACCAGCCAAG GCAAGGTGGC CCTGGGCAGC CTCTTCCATG GCCTGGACGT GGTTTTCCTG 
CAGCCAACCT CCTTGACCCT GCTGTACCCT CTGGCCTCGC CCTCCAATAG CACTGACGTC 
TATGTGGAGC CCATGGAGAT CGCCACCTTC CGCCTCCGCT TGGGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAN2A2 ( XM_012133339.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_011988729.1 Ovis aries musimon mannosidase, alpha, class 2A, member 2 (MAN2A2), transcript variant X4, mRNA.

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CloneID	OOv15681
Clone ID Related Accession (Same CDS sequence)	XM_012133339.2
Accession Version	XM_012133339.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3453bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	alpha-mannosidase 2x isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942386.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012133339.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCTGA AAAAGCAGGT GACAGTGTGT GGGGCTGCTA TCTTCTGCGT GGCTGTCTTC 
TCGCTCTACC TCATGTTGGA CCGAGTACAG CATGATCCTG CTCGACACCA GAACGGTGGG 
AACTTCCCCC GGAGTCAGAT ATCTGTGCTA CAGAACCGCA TTGAACAGCT GGAACAGCTG 
TTGGAGGAGA ACCATGAGAT CATCAGCCAC ATCAAAGACT CTGTGCTGGA GCTGACAGCC 
AACGTGGAGG GCCCACCCGC CCTGCTGCCC TACTATGTGG CCAACGGCTC CTGGGTGGTG 
CCGCCAGAGC CCCGGCCCAG CTTCTTCTCC ATCTCCCCTC AAGACTGCCA GTTTGCTTTG 
GGGGGCCGCG GCGAGAAGCC AGAGCTGCAG ATGCTGACTG TATCAGAGGA GTTACCGTTT 
GACAACGTGG ATGGCGGCGT GTGGAAGCAA GGCTTCGACA TTTCCTACAG CCCGCACGAC 
TGGGACACCG AAGACCTGCA GGTGTTTGTG GTCCCCCACT CTCACAATGA CCCCGGCTGG 
CTCAAGACCT TTGACAAGTA CTACACAGAA CAGACCCAGC ACATCCTCAA CAACATGGTG 
TCCAAGCTGC AGGAGGACCC CCGGCGGCGC TTCATCTGGG CAGAAGTCTC CTTCTTTGCC 
AAGTGGTGGG ACAACATCAG TGCCCAAAAG AGAGCAGCAG TCCGAAGGCT GGTGGGAAAC 
GGGCAGCTGG AGATTGCAAC AGGAGGCTGG GTGATGCCGG ACGAGGCCAA CTCCCACTAT 
TTTGCATTGA TTGACCAGCT CATTGAGGGC CACCAGTGGC TGGAGAAGAA CCTGGGTGCA 
ACTCCGCGCT CGGGCTGGGC GGTGGACCCC TTTGGATACA GCCCCACCAT GCCTTACCTG 
CTGCGCCGTG CCAACCTGAC CAGCATGCTC ATTCAGAGGG TACACTACGC CATCAAGAAG 
CACTTTGCTG CCAGCCACAG CCTGGAGTTC ATGTGGAGGC AGACCTGGGA CTCGGATGCC 
AGCACAGACA TCTTCTGCCA CATGATGCCT TTCTACAGCT ACGATGTCCC CCATACGTGT 
GGCCCGGATC CCAAGATCTG CTGCCAGTTC GATTTCAAGC GCTTGCCTGG CGGGCGTATC 
AGCTGCCCTT GGAAGGTGCC GCCCCGGGCT ATCACGGAGG CCAATGTGGC TGAGAGGGCA 
GGCCTGCTCC TGGACCAGTA CAGGAAGAAG TCCCGGCTCT TCCGGAGCAA TGTGCTCCTG 
GTGCCGCTGG GCGATGACTT CCGCTACGAC AAGCCCCAGG AGTGGGACGC CCAGTTCTTC 
AACTACCAGC GGCTCTTCGA CTTCCTCAAC AGCAAGCCTG ACCTCCATGT GCAGGCCCAG 
TTTGGCACCC TCTCCGACTA TTTTGACGCT CTATACAAGA GGACAGGGGT GGAGCCGGGA 
GCCCGGCCTC CAGGTTTCCC TGTGCTGAGT GGGGATTTCT TCTCCTACGC GGACCGGGAG 
GACCACTACT GGACAGGCTA TTACACTTCA CGGCCTTTCT ACAAGAACCT GGACCGCGTC 
CTGGAAGCAC ACCTGCGGGG TGCAGAGATT CTGTACAGCC TGGCCGTGGC TCACGCCCGC 
CGCGCTGGAC TGGCCAGCCA GTTCCCACTC TCAAACTTCG CCCTTCTGAC GGACGCTCGG 
CGCACACTGG GGCTCTTCCA GCACCATGAC GCAATCACGG GCACCGCCAA GGAGGCCGTT 
GTGGTGGACT ATGGGGTCAG GCTTCTGCGC TCCCTTGTCA GCCTGAAACA GGTCATCATG 
AACGCAGCTC ACTACCTGGT GCTGGGGGAC AAGAAGGCCT ACCACTTTGA CCCAGAGGTG 
CCCTTCCTGC AGATGGATGA CACCCGCTTA AATCACGACG CCCTTCCAGA GCGCACAGTG 
ATCCAGCTGG AATCCTCACC CAGGTATGTG GTGCTGTTCA ACCCACTGGA GCAGGAGCGG 
CTCAGCGTGG TGTCCCTGCT GGTGAGCTCA CCCCGGGTGC GCGTGCTTTC GGAGGAGGGT 
CAGCCCCTGG CCGTGCAGGT CAGCGCGCAC TGGAGCTCTG CCACCGACAT GGTCCCTGAC 
GTCTATCAGG TGTCTGTGCC TGTCCGCCTG CCAGCGCTGG GTCTTGGCGT GCTGCAGCTG 
CAGCAGGGCC TGGATGGGCC CCGCACGCTG CCCTCCTCTG TGCGCGTCTA CCTACATGGG 
CGGCCGCTGT CCGTCAGCAG GAACGAGGCG TTCCCTCTCC GTGTCATTGA CTCTGGCACC 
AGTGACTTCG CCCTCAGCAA CCGCTACATG CAGGTCTGGT TCTCAGGCCT TACCGGGCTC 
CTCAAGAGCG TCCGAAGGGT GGATGAGGAG CAGGAACAGC GGGTGGACAT GGAGTTCCTC 
GTCTATGGCA CCCGCTCGTC CAAAGACAAG AGTGGAGCCT ACCTCTTCCT GCCTGACGGC 
GAGGCCAAGC CCTACGTCCC CAGGGACCCA CCCGTGCTGC GCGTCACAGA AGGCCCTTTC 
TTCTCAGAGG TGGTTGCCTG CTACGAGCAT GTTCACCAGG TGGTCCGGCT GTATAACCTG 
CCGGGGGTGG AGGGGCTGTC CCTGGATGTG TCGTCCTTGG TGGACATCCG GGACTACGTC 
AACAAGGAGC TGGCCCTGCG CATTCGCACT GACATCGATA GCCAGGGCAT CTTCTTCACA 
GACCTCAACG GCTTTCAGGT GCAGCCCCGA CGGTATCTGA AGAAGCTGCC CCTGCAGGCC 
AACTTCTACC CCATGCCCGT CATGGCCTAC CTCCAGGACG CTCAGAACCG CCTCACGCTG 
CACACAGCCC AGGCCCTGGG TGTCTCCAGC CTCCACAATG GCCAGCTAGA GGTGATCTTG 
GACCGGAGAC TGATGCAGGA CGACAACCGG GGCCTTGGCC AAGGGCTCAA GGACAACAAG 
AGAACCTGCA ACCACTTCCG CCTCCTGTTG GAACGGCGAA CCCTGGGCAG GGAGGTCCGC 
GAGGGCTCCT CTGCAAGCTT CCCGTCCCTC CTCAGCCACC TGACCTCCAT GTACCTGAAC 
ACCCCTGTGC TCGCCCTGCC TGTGGCCAGG AGGCAGGCCC CCGGCCCCGC TCTGCGCTCT 
TTTCACCCTC TGGCCTCCTC ACTGCCCTGT GACTTCCACC TGCTCAACCT GCGGATGCTC 
CAGGCCGAGG AGGATGGCTT GCCCTCGGCA GAAGCTGCGC TCCTCTTACA CCGCAAGGGT 
TTTGACTGTG GCCTTGAGGC CAAGAACTTG GGCTTCAACT GCACCACCAG CCAAGGCAAG 
GTGGCCCTGG GCAGCCTCTT CCATGGCCTG GACGTGGTTT TCCTGCAGCC AACCTCCTTG 
ACCCTGCTGT ACCCTCTGGC CTCGCCCTCC AATAGCACTG ACGTCTATGT GGAGCCCATG 
GAGATCGCCA CCTTCCGCCT CCGCTTGGGC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011988729.1
CDS	433..3885
Translation	MKLKKQVTVCGAAIFCVAVFSLYLMLDRVQHDPARHQNGGNFPRSQISVLQNRIEQLEQLLEENHEIISHIKDSVLELTANVEGPPALLPYYVANGSWVVPPEPRPSFFSISPQDCQFALGGRGEKPELQMLTVSEELPFDNVDGGVWKQGFDISYSPHDWDTEDLQVFVVPHSHNDPGWLKTFDKYYTEQTQHILNNMVSKLQEDPRRRFIWAEVSFFAKWWDNISAQKRAAVRRLVGNGQLEIATGGWVMPDEANSHYFALIDQLIEGHQWLEKNLGATPRSGWAVDPFGYSPTMPYLLRRANLTSMLIQRVHYAIKKHFAASHSLEFMWRQTWDSDASTDIFCHMMPFYSYDVPHTCGPDPKICCQFDFKRLPGGRISCPWKVPPRAITEANVAERAGLLLDQYRKKSRLFRSNVLLVPLGDDFRYDKPQEWDAQFFNYQRLFDFLNSKPDLHVQAQFGTLSDYFDALYKRTGVEPGARPPGFPVLSGDFFSYADREDHYWTGYYTSRPFYKNLDRVLEAHLRGAEILYSLAVAHARRAGLASQFPLSNFALLTDARRTLGLFQHHDAITGTAKEAVVVDYGVRLLRSLVSLKQVIMNAAHYLVLGDKKAYHFDPEVPFLQMDDTRLNHDALPERTVIQLESSPRYVVLFNPLEQERLSVVSLLVSSPRVRVLSEEGQPLAVQVSAHWSSATDMVPDVYQVSVPVRLPALGLGVLQLQQGLDGPRTLPSSVRVYLHGRPLSVSRNEAFPLRVIDSGTSDFALSNRYMQVWFSGLTGLLKSVRRVDEEQEQRVDMEFLVYGTRSSKDKSGAYLFLPDGEAKPYVPRDPPVLRVTEGPFFSEVVACYEHVHQVVRLYNLPGVEGLSLDVSSLVDIRDYVNKELALRIRTDIDSQGIFFTDLNGFQVQPRRYLKKLPLQANFYPMPVMAYLQDAQNRLTLHTAQALGVSSLHNGQLEVILDRRLMQDDNRGLGQGLKDNKRTCNHFRLLLERRTLGREVREGSSASFPSLLSHLTSMYLNTPVLALPVARRQAPGPALRSFHPLASSLPCDFHLLNLRMLQAEEDGLPSAEAALLLHRKGFDCGLEAKNLGFNCTTSQGKVALGSLFHGLDVVFLQPTSLTLLYPLASPSNSTDVYVEPMEIATFRLRLG

Target ORF information:

RefSeq Version	XM_012133339.2
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon mannosidase, alpha, class 2A, member 2 (MAN2A2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012133339.2

ORF Insert Sequence:

ATGAAGCTGA AAAAGCAGGT GACAGTGTGT GGGGCTGCTA TCTTCTGCGT GGCTGTCTTC 
TCGCTCTACC TCATGTTGGA CCGAGTACAG CATGATCCTG CTCGACACCA GAACGGTGGG 
AACTTCCCCC GGAGTCAGAT ATCTGTGCTA CAGAACCGCA TTGAACAGCT GGAACAGCTG 
TTGGAGGAGA ACCATGAGAT CATCAGCCAC ATCAAAGACT CTGTGCTGGA GCTGACAGCC 
AACGTGGAGG GCCCACCCGC CCTGCTGCCC TACTATGTGG CCAACGGCTC CTGGGTGGTG 
CCGCCAGAGC CCCGGCCCAG CTTCTTCTCC ATCTCCCCTC AAGACTGCCA GTTTGCTTTG 
GGGGGCCGCG GCGAGAAGCC AGAGCTGCAG ATGCTGACTG TATCAGAGGA GTTACCGTTT 
GACAACGTGG ATGGCGGCGT GTGGAAGCAA GGCTTCGACA TTTCCTACAG CCCGCACGAC 
TGGGACACCG AAGACCTGCA GGTGTTTGTG GTCCCCCACT CTCACAATGA CCCCGGCTGG 
CTCAAGACCT TTGACAAGTA CTACACAGAA CAGACCCAGC ACATCCTCAA CAACATGGTG 
TCCAAGCTGC AGGAGGACCC CCGGCGGCGC TTCATCTGGG CAGAAGTCTC CTTCTTTGCC 
AAGTGGTGGG ACAACATCAG TGCCCAAAAG AGAGCAGCAG TCCGAAGGCT GGTGGGAAAC 
GGGCAGCTGG AGATTGCAAC AGGAGGCTGG GTGATGCCGG ACGAGGCCAA CTCCCACTAT 
TTTGCATTGA TTGACCAGCT CATTGAGGGC CACCAGTGGC TGGAGAAGAA CCTGGGTGCA 
ACTCCGCGCT CGGGCTGGGC GGTGGACCCC TTTGGATACA GCCCCACCAT GCCTTACCTG 
CTGCGCCGTG CCAACCTGAC CAGCATGCTC ATTCAGAGGG TACACTACGC CATCAAGAAG 
CACTTTGCTG CCAGCCACAG CCTGGAGTTC ATGTGGAGGC AGACCTGGGA CTCGGATGCC 
AGCACAGACA TCTTCTGCCA CATGATGCCT TTCTACAGCT ACGATGTCCC CCATACGTGT 
GGCCCGGATC CCAAGATCTG CTGCCAGTTC GATTTCAAGC GCTTGCCTGG CGGGCGTATC 
AGCTGCCCTT GGAAGGTGCC GCCCCGGGCT ATCACGGAGG CCAATGTGGC TGAGAGGGCA 
GGCCTGCTCC TGGACCAGTA CAGGAAGAAG TCCCGGCTCT TCCGGAGCAA TGTGCTCCTG 
GTGCCGCTGG GCGATGACTT CCGCTACGAC AAGCCCCAGG AGTGGGACGC CCAGTTCTTC 
AACTACCAGC GGCTCTTCGA CTTCCTCAAC AGCAAGCCTG ACCTCCATGT GCAGGCCCAG 
TTTGGCACCC TCTCCGACTA TTTTGACGCT CTATACAAGA GGACAGGGGT GGAGCCGGGA 
GCCCGGCCTC CAGGTTTCCC TGTGCTGAGT GGGGATTTCT TCTCCTACGC GGACCGGGAG 
GACCACTACT GGACAGGCTA TTACACTTCA CGGCCTTTCT ACAAGAACCT GGACCGCGTC 
CTGGAAGCAC ACCTGCGGGG TGCAGAGATT CTGTACAGCC TGGCCGTGGC TCACGCCCGC 
CGCGCTGGAC TGGCCAGCCA GTTCCCACTC TCAAACTTCG CCCTTCTGAC GGACGCTCGG 
CGCACACTGG GGCTCTTCCA GCACCATGAC GCAATCACGG GCACCGCCAA GGAGGCCGTT 
GTGGTGGACT ATGGGGTCAG GCTTCTGCGC TCCCTTGTCA GCCTGAAACA GGTCATCATG 
AACGCAGCTC ACTACCTGGT GCTGGGGGAC AAGAAGGCCT ACCACTTTGA CCCAGAGGTG 
CCCTTCCTGC AGATGGATGA CACCCGCTTA AATCACGACG CCCTTCCAGA GCGCACAGTG 
ATCCAGCTGG AATCCTCACC CAGGTATGTG GTGCTGTTCA ACCCACTGGA GCAGGAGCGG 
CTCAGCGTGG TGTCCCTGCT GGTGAGCTCA CCCCGGGTGC GCGTGCTTTC GGAGGAGGGT 
CAGCCCCTGG CCGTGCAGGT CAGCGCGCAC TGGAGCTCTG CCACCGACAT GGTCCCTGAC 
GTCTATCAGG TGTCTGTGCC TGTCCGCCTG CCAGCGCTGG GTCTTGGCGT GCTGCAGCTG 
CAGCAGGGCC TGGATGGGCC CCGCACGCTG CCCTCCTCTG TGCGCGTCTA CCTACATGGG 
CGGCCGCTGT CCGTCAGCAG GAACGAGGCG TTCCCTCTCC GTGTCATTGA CTCTGGCACC 
AGTGACTTCG CCCTCAGCAA CCGCTACATG CAGGTCTGGT TCTCAGGCCT TACCGGGCTC 
CTCAAGAGCG TCCGAAGGGT GGATGAGGAG CAGGAACAGC GGGTGGACAT GGAGTTCCTC 
GTCTATGGCA CCCGCTCGTC CAAAGACAAG AGTGGAGCCT ACCTCTTCCT GCCTGACGGC 
GAGGCCAAGC CCTACGTCCC CAGGGACCCA CCCGTGCTGC GCGTCACAGA AGGCCCTTTC 
TTCTCAGAGG TGGTTGCCTG CTACGAGCAT GTTCACCAGG TGGTCCGGCT GTATAACCTG 
CCGGGGGTGG AGGGGCTGTC CCTGGATGTG TCGTCCTTGG TGGACATCCG GGACTACGTC 
AACAAGGAGC TGGCCCTGCG CATTCGCACT GACATCGATA GCCAGGGCAT CTTCTTCACA 
GACCTCAACG GCTTTCAGGT GCAGCCCCGA CGGTATCTGA AGAAGCTGCC CCTGCAGGCC 
AACTTCTACC CCATGCCCGT CATGGCCTAC CTCCAGGACG CTCAGAACCG CCTCACGCTG 
CACACAGCCC AGGCCCTGGG TGTCTCCAGC CTCCACAATG GCCAGCTAGA GGTGATCTTG 
GACCGGAGAC TGATGCAGGA CGACAACCGG GGCCTTGGCC AAGGGCTCAA GGACAACAAG 
AGAACCTGCA ACCACTTCCG CCTCCTGTTG GAACGGCGAA CCCTGGGCAG GGAGGTCCGC 
GAGGGCTCCT CTGCAAGCTT CCCGTCCCTC CTCAGCCACC TGACCTCCAT GTACCTGAAC 
ACCCCTGTGC TCGCCCTGCC TGTGGCCAGG AGGCAGGCCC CCGGCCCCGC TCTGCGCTCT 
TTTCACCCTC TGGCCTCCTC ACTGCCCTGT GACTTCCACC TGCTCAACCT GCGGATGCTC 
CAGGCCGAGG AGGATGGCTT GCCCTCGGCA GAAGCTGCGC TCCTCTTACA CCGCAAGGGT 
TTTGACTGTG GCCTTGAGGC CAAGAACTTG GGCTTCAACT GCACCACCAG CCAAGGCAAG 
GTGGCCCTGG GCAGCCTCTT CCATGGCCTG GACGTGGTTT TCCTGCAGCC AACCTCCTTG 
ACCCTGCTGT ACCCTCTGGC CTCGCCCTCC AATAGCACTG ACGTCTATGT GGAGCCCATG 
GAGATCGCCA CCTTCCGCCT CCGCTTGGGC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAN2A2 ( XM_012133340.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_011988730.1 Ovis aries musimon mannosidase, alpha, class 2A, member 2 (MAN2A2), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OOv15682
Clone ID Related Accession (Same CDS sequence)	XM_012133340.2
Accession Version	XM_012133340.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3402bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	alpha-mannosidase 2x isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942386.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012133340.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCTGA AAAAGCAGGT GACAGTGTGT GGGGCTGCTA TCTTCTGCGT GGCTGTCTTC 
TCGCTCTACC TCATGTTGGA CCGAGTACAG CATGATCCTG CTCGACACCA GAACGGTGGG 
AACTTCCCCC GGAGTCAGAT ATCTGTGCTA CAGAACCGCA TTGAACAGCT GGAACAGCTG 
TTGGAGGAGA ACCATGAGAT CATCAGCCAC ATCAAAGACT CTGTGCTGGA GCTGACAGCC 
AACGTGGAGG GCCCACCCGC CCTGCTGCCC TACTATGTGG CCAACGGCTC CTGGGTGGTG 
CCGCCAGAGC CCCGGCCCAG CTTCTTCTCC ATCTCCCCTC AAGACTGCCA GTTTGCTTTG 
GGGGGCCGCG GCGAGAAGCC AGAGCTGCAG ATGCTGACTG TATCAGAGGA GTTACCGTTT 
GACAACGTGG ATGGCGGCGT GTGGAAGCAA GGCTTCGACA TTTCCTACAG CCCGCACGAC 
TGGGACACCG AAGACCTGCA GGTGTTTGTG GTCCCCCACT CTCACAATGA CCCCGGCTGG 
CTCAAGACCT TTGACAAGTA CTACACAGAA CAGACCCAGC ACATCCTCAA CAACATGGTG 
TCCAAGCTGC AGGAGGACCC CCGGCGGCGC TTCATCTGGG CAGAAGTCTC CTTCTTTGCC 
AAGTGGTGGG ACAACATCAG TGCCCAAAAG AGAGCAGCAG TCCGAAGGCT GGTGGGAAAC 
GGGCAGCTGG AGATTGCAAC AGGAGGCTGG GTGATGCCGG ACGAGGCCAA CTCCCACTAT 
TTTGCATTGA TTGACCAGCT CATTGAGGGC CACCAGTGGC TGGAGAAGAA CCTGGGTGCA 
ACTCCGCGCT CGGGCTGGGC GGTGGACCCC TTTGGATACA GCCCCACCAT GCCTTACCTG 
CTGCGCCGTG CCAACCTGAC CAGCATGCTC ATTCAGAGGG TACACTACGC CATCAAGAAG 
CACTTTGCTG CCAGCCACAG CCTGGAGTTC ATGTGGAGGC AGACCTGGGA CTCGGATGCC 
AGCACAGACA TCTTCTGCCA CATGATGCCT TTCTACAGCT ACGATGTCCC CCATACGTGT 
GGCCCGGATC CCAAGATCTG CTGCCAGTTC GATTTCAAGC GCTTGCCTGG CGGGCGTATC 
AGCTGCCCTT GGAAGGTGCC GCCCCGGGCT ATCACGGAGG CCAATGTGGC TGAGAGGGCA 
GGCCTGCTCC TGGACCAGTA CAGGAAGAAG TCCCGGCTCT TCCGGAGCAA TGTGCTCCTG 
GTGCCGCTGG GCGATGACTT CCGCTACGAC AAGCCCCAGG AGTGGGACGC CCAGTTCTTC 
AACTACCAGC GGCTCTTCGA CTTCCTCAAC AGCAAGCCTG ACCTCCATGT GCAGGACCAC 
TACTGGACAG GCTATTACAC TTCACGGCCT TTCTACAAGA ACCTGGACCG CGTCCTGGAA 
GCACACCTGC GGGGTGCAGA GATTCTGTAC AGCCTGGCCG TGGCTCACGC CCGCCGCGCT 
GGACTGGCCA GCCAGTTCCC ACTCTCAAAC TTCGCCCTTC TGACGGACGC TCGGCGCACA 
CTGGGGCTCT TCCAGCACCA TGACGCAATC ACGGGCACCG CCAAGGAGGC CGTTGTGGTG 
GACTATGGGG TCAGGCTTCT GCGCTCCCTT GTCAGCCTGA AACAGGTCAT CATGAACGCA 
GCTCACTACC TGGTGCTGGG GGACAAGAAG GCCTACCACT TTGACCCAGA GGTGCCCTTC 
CTGCAGATGG ATGACACCCG CTTAAATCAC GACGCCCTTC CAGAGCGCAC AGTGATCCAG 
CTGGAATCCT CACCCAGGTA TGTGGTGCTG TTCAACCCAC TGGAGCAGGA GCGGCTCAGC 
GTGGTGTCCC TGCTGGTGAG CTCACCCCGG GTGCGCGTGC TTTCGGAGGA GGGTCAGCCC 
CTGGCCGTGC AGGTCAGCGC GCACTGGAGC TCTGCCACCG ACATGGTCCC TGACGTCTAT 
CAGGTGTCTG TGCCTGTCCG CCTGCCAGCG CTGGGTCTTG GCGTGCTGCA GCTGCAGCAG 
GGCCTGGATG GGCCCCGCAC GCTGCCCTCC TCTGTGCGCG TCTACCTACA TGGGCGGCCG 
CTGTCCGTCA GCAGGAACGA GGCGTTCCCT CTCCGTGTCA TTGACTCTGG CACCAGTGAC 
TTCGCCCTCA GCAACCGCTA CATGCAGGTC TGGTTCTCAG GCCTTACCGG GCTCCTCAAG 
AGCGTCCGAA GGGTGGATGA GGAGCAGGAA CAGCGGGTGG ACATGGAGTT CCTCGTCTAT 
GGCACCCGCT CGTCCAAAGA CAAGAGTGGA GCCTACCTCT TCCTGCCTGA CGGCGAGGCC 
AAGCCCTACG TCCCCAGGGA CCCACCCGTG CTGCGCGTCA CAGAAGGCCC TTTCTTCTCA 
GAGGTGGTTG CCTGCTACGA GCATGTTCAC CAGGTGGTCC GGCTGTATAA CCTGCCGGGG 
GTGGAGGGGC TGTCCCTGGA TGTGTCGTCC TTGGTGGACA TCCGGGACTA CGTCAACAAG 
GAGCTGGCCC TGCGCATTCG CACTGACATC GATAGCCAGG GCATCTTCTT CACAGACCTC 
AACGGCTTTC AGGTGCAGCC CCGACGGTAT CTGAAGAAGC TGCCCCTGCA GGCCAACTTC 
TACCCCATGC CCGTCATGGC CTACCTCCAG GACGCTCAGA ACCGCCTCAC GCTGCACACA 
GCCCAGGCCC TGGGTGTCTC CAGCCTCCAC AATGGCCAGC TAGAGGTGAT CTTGGACCGG 
AGACTGATGC AGGACGACAA CCGGGGCCTT GGCCAAGGGC TCAAGGACAA CAAGAGAACC 
TGCAACCACT TCCGCCTCCT GTTGGAACGG CGAACCCTGG GCAGGGAGCC TGGCTTTTTC 
TCCAAACTGG CAGCCATGTT TAGGGGCTTG ATCTTTCACA GCAGCAGGAA CAGGAACCGA 
GAGGTCCGCG AGGGCTCCTC TGCAAGCTTC CCGTCCCTCC TCAGCCACCT GACCTCCATG 
TACCTGAACA CCCCTGTGCT CGCCCTGCCT GTGGCCAGGA GGCAGGCCCC CGGCCCCGCT 
CTGCGCTCTT TTCACCCTCT GGCCTCCTCA CTGCCCTGTG ACTTCCACCT GCTCAACCTG 
CGGATGCTCC AGGCCGAGGA GGATGGCTTG CCCTCGGCAG AAGCTGCGCT CCTCTTACAC 
CGCAAGGGTT TTGACTGTGG CCTTGAGGCC AAGAACTTGG GCTTCAACTG CACCACCAGC 
CAAGGCAAGG TGGCCCTGGG CAGCCTCTTC CATGGCCTGG ACGTGGTTTT CCTGCAGCCA 
ACCTCCTTGA CCCTGCTGTA CCCTCTGGCC TCGCCCTCCA ATAGCACTGA CGTCTATGTG 
GAGCCCATGG AGATCGCCAC CTTCCGCCTC CGCTTGGGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011988730.1
CDS	442..3843
Translation	MKLKKQVTVCGAAIFCVAVFSLYLMLDRVQHDPARHQNGGNFPRSQISVLQNRIEQLEQLLEENHEIISHIKDSVLELTANVEGPPALLPYYVANGSWVVPPEPRPSFFSISPQDCQFALGGRGEKPELQMLTVSEELPFDNVDGGVWKQGFDISYSPHDWDTEDLQVFVVPHSHNDPGWLKTFDKYYTEQTQHILNNMVSKLQEDPRRRFIWAEVSFFAKWWDNISAQKRAAVRRLVGNGQLEIATGGWVMPDEANSHYFALIDQLIEGHQWLEKNLGATPRSGWAVDPFGYSPTMPYLLRRANLTSMLIQRVHYAIKKHFAASHSLEFMWRQTWDSDASTDIFCHMMPFYSYDVPHTCGPDPKICCQFDFKRLPGGRISCPWKVPPRAITEANVAERAGLLLDQYRKKSRLFRSNVLLVPLGDDFRYDKPQEWDAQFFNYQRLFDFLNSKPDLHVQDHYWTGYYTSRPFYKNLDRVLEAHLRGAEILYSLAVAHARRAGLASQFPLSNFALLTDARRTLGLFQHHDAITGTAKEAVVVDYGVRLLRSLVSLKQVIMNAAHYLVLGDKKAYHFDPEVPFLQMDDTRLNHDALPERTVIQLESSPRYVVLFNPLEQERLSVVSLLVSSPRVRVLSEEGQPLAVQVSAHWSSATDMVPDVYQVSVPVRLPALGLGVLQLQQGLDGPRTLPSSVRVYLHGRPLSVSRNEAFPLRVIDSGTSDFALSNRYMQVWFSGLTGLLKSVRRVDEEQEQRVDMEFLVYGTRSSKDKSGAYLFLPDGEAKPYVPRDPPVLRVTEGPFFSEVVACYEHVHQVVRLYNLPGVEGLSLDVSSLVDIRDYVNKELALRIRTDIDSQGIFFTDLNGFQVQPRRYLKKLPLQANFYPMPVMAYLQDAQNRLTLHTAQALGVSSLHNGQLEVILDRRLMQDDNRGLGQGLKDNKRTCNHFRLLLERRTLGREPGFFSKLAAMFRGLIFHSSRNRNREVREGSSASFPSLLSHLTSMYLNTPVLALPVARRQAPGPALRSFHPLASSLPCDFHLLNLRMLQAEEDGLPSAEAALLLHRKGFDCGLEAKNLGFNCTTSQGKVALGSLFHGLDVVFLQPTSLTLLYPLASPSNSTDVYVEPMEIATFRLRLG

Target ORF information:

RefSeq Version	XM_012133340.2
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon mannosidase, alpha, class 2A, member 2 (MAN2A2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012133340.2

ORF Insert Sequence:

ATGAAGCTGA AAAAGCAGGT GACAGTGTGT GGGGCTGCTA TCTTCTGCGT GGCTGTCTTC 
TCGCTCTACC TCATGTTGGA CCGAGTACAG CATGATCCTG CTCGACACCA GAACGGTGGG 
AACTTCCCCC GGAGTCAGAT ATCTGTGCTA CAGAACCGCA TTGAACAGCT GGAACAGCTG 
TTGGAGGAGA ACCATGAGAT CATCAGCCAC ATCAAAGACT CTGTGCTGGA GCTGACAGCC 
AACGTGGAGG GCCCACCCGC CCTGCTGCCC TACTATGTGG CCAACGGCTC CTGGGTGGTG 
CCGCCAGAGC CCCGGCCCAG CTTCTTCTCC ATCTCCCCTC AAGACTGCCA GTTTGCTTTG 
GGGGGCCGCG GCGAGAAGCC AGAGCTGCAG ATGCTGACTG TATCAGAGGA GTTACCGTTT 
GACAACGTGG ATGGCGGCGT GTGGAAGCAA GGCTTCGACA TTTCCTACAG CCCGCACGAC 
TGGGACACCG AAGACCTGCA GGTGTTTGTG GTCCCCCACT CTCACAATGA CCCCGGCTGG 
CTCAAGACCT TTGACAAGTA CTACACAGAA CAGACCCAGC ACATCCTCAA CAACATGGTG 
TCCAAGCTGC AGGAGGACCC CCGGCGGCGC TTCATCTGGG CAGAAGTCTC CTTCTTTGCC 
AAGTGGTGGG ACAACATCAG TGCCCAAAAG AGAGCAGCAG TCCGAAGGCT GGTGGGAAAC 
GGGCAGCTGG AGATTGCAAC AGGAGGCTGG GTGATGCCGG ACGAGGCCAA CTCCCACTAT 
TTTGCATTGA TTGACCAGCT CATTGAGGGC CACCAGTGGC TGGAGAAGAA CCTGGGTGCA 
ACTCCGCGCT CGGGCTGGGC GGTGGACCCC TTTGGATACA GCCCCACCAT GCCTTACCTG 
CTGCGCCGTG CCAACCTGAC CAGCATGCTC ATTCAGAGGG TACACTACGC CATCAAGAAG 
CACTTTGCTG CCAGCCACAG CCTGGAGTTC ATGTGGAGGC AGACCTGGGA CTCGGATGCC 
AGCACAGACA TCTTCTGCCA CATGATGCCT TTCTACAGCT ACGATGTCCC CCATACGTGT 
GGCCCGGATC CCAAGATCTG CTGCCAGTTC GATTTCAAGC GCTTGCCTGG CGGGCGTATC 
AGCTGCCCTT GGAAGGTGCC GCCCCGGGCT ATCACGGAGG CCAATGTGGC TGAGAGGGCA 
GGCCTGCTCC TGGACCAGTA CAGGAAGAAG TCCCGGCTCT TCCGGAGCAA TGTGCTCCTG 
GTGCCGCTGG GCGATGACTT CCGCTACGAC AAGCCCCAGG AGTGGGACGC CCAGTTCTTC 
AACTACCAGC GGCTCTTCGA CTTCCTCAAC AGCAAGCCTG ACCTCCATGT GCAGGACCAC 
TACTGGACAG GCTATTACAC TTCACGGCCT TTCTACAAGA ACCTGGACCG CGTCCTGGAA 
GCACACCTGC GGGGTGCAGA GATTCTGTAC AGCCTGGCCG TGGCTCACGC CCGCCGCGCT 
GGACTGGCCA GCCAGTTCCC ACTCTCAAAC TTCGCCCTTC TGACGGACGC TCGGCGCACA 
CTGGGGCTCT TCCAGCACCA TGACGCAATC ACGGGCACCG CCAAGGAGGC CGTTGTGGTG 
GACTATGGGG TCAGGCTTCT GCGCTCCCTT GTCAGCCTGA AACAGGTCAT CATGAACGCA 
GCTCACTACC TGGTGCTGGG GGACAAGAAG GCCTACCACT TTGACCCAGA GGTGCCCTTC 
CTGCAGATGG ATGACACCCG CTTAAATCAC GACGCCCTTC CAGAGCGCAC AGTGATCCAG 
CTGGAATCCT CACCCAGGTA TGTGGTGCTG TTCAACCCAC TGGAGCAGGA GCGGCTCAGC 
GTGGTGTCCC TGCTGGTGAG CTCACCCCGG GTGCGCGTGC TTTCGGAGGA GGGTCAGCCC 
CTGGCCGTGC AGGTCAGCGC GCACTGGAGC TCTGCCACCG ACATGGTCCC TGACGTCTAT 
CAGGTGTCTG TGCCTGTCCG CCTGCCAGCG CTGGGTCTTG GCGTGCTGCA GCTGCAGCAG 
GGCCTGGATG GGCCCCGCAC GCTGCCCTCC TCTGTGCGCG TCTACCTACA TGGGCGGCCG 
CTGTCCGTCA GCAGGAACGA GGCGTTCCCT CTCCGTGTCA TTGACTCTGG CACCAGTGAC 
TTCGCCCTCA GCAACCGCTA CATGCAGGTC TGGTTCTCAG GCCTTACCGG GCTCCTCAAG 
AGCGTCCGAA GGGTGGATGA GGAGCAGGAA CAGCGGGTGG ACATGGAGTT CCTCGTCTAT 
GGCACCCGCT CGTCCAAAGA CAAGAGTGGA GCCTACCTCT TCCTGCCTGA CGGCGAGGCC 
AAGCCCTACG TCCCCAGGGA CCCACCCGTG CTGCGCGTCA CAGAAGGCCC TTTCTTCTCA 
GAGGTGGTTG CCTGCTACGA GCATGTTCAC CAGGTGGTCC GGCTGTATAA CCTGCCGGGG 
GTGGAGGGGC TGTCCCTGGA TGTGTCGTCC TTGGTGGACA TCCGGGACTA CGTCAACAAG 
GAGCTGGCCC TGCGCATTCG CACTGACATC GATAGCCAGG GCATCTTCTT CACAGACCTC 
AACGGCTTTC AGGTGCAGCC CCGACGGTAT CTGAAGAAGC TGCCCCTGCA GGCCAACTTC 
TACCCCATGC CCGTCATGGC CTACCTCCAG GACGCTCAGA ACCGCCTCAC GCTGCACACA 
GCCCAGGCCC TGGGTGTCTC CAGCCTCCAC AATGGCCAGC TAGAGGTGAT CTTGGACCGG 
AGACTGATGC AGGACGACAA CCGGGGCCTT GGCCAAGGGC TCAAGGACAA CAAGAGAACC 
TGCAACCACT TCCGCCTCCT GTTGGAACGG CGAACCCTGG GCAGGGAGCC TGGCTTTTTC 
TCCAAACTGG CAGCCATGTT TAGGGGCTTG ATCTTTCACA GCAGCAGGAA CAGGAACCGA 
GAGGTCCGCG AGGGCTCCTC TGCAAGCTTC CCGTCCCTCC TCAGCCACCT GACCTCCATG 
TACCTGAACA CCCCTGTGCT CGCCCTGCCT GTGGCCAGGA GGCAGGCCCC CGGCCCCGCT 
CTGCGCTCTT TTCACCCTCT GGCCTCCTCA CTGCCCTGTG ACTTCCACCT GCTCAACCTG 
CGGATGCTCC AGGCCGAGGA GGATGGCTTG CCCTCGGCAG AAGCTGCGCT CCTCTTACAC 
CGCAAGGGTT TTGACTGTGG CCTTGAGGCC AAGAACTTGG GCTTCAACTG CACCACCAGC 
CAAGGCAAGG TGGCCCTGGG CAGCCTCTTC CATGGCCTGG ACGTGGTTTT CCTGCAGCCA 
ACCTCCTTGA CCCTGCTGTA CCCTCTGGCC TCGCCCTCCA ATAGCACTGA CGTCTATGTG 
GAGCCCATGG AGATCGCCAC CTTCCGCCTC CGCTTGGGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAN2A2 ( XM_012133341.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_011988731.1 Ovis aries musimon mannosidase, alpha, class 2A, member 2 (MAN2A2), transcript variant X6, mRNA.

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CloneID	OOv15683
Clone ID Related Accession (Same CDS sequence)	XM_012133341.2
Accession Version	XM_012133341.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3327bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	alpha-mannosidase 2x isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942386.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012133341.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCTGA AAAAGCAGGT GACAGTGTGT GGGGCTGCTA TCTTCTGCGT GGCTGTCTTC 
TCGCTCTACC TCATGTTGGA CCGAGTACAG CATGATCCTG CTCGACACCA GAACGGTGGG 
AACTTCCCCC GGAGTCAGAT ATCTGTGCTA CAGAACCGCA TTGAACAGCT GGAACAGCTG 
TTGGAGGAGA ACCATGAGAT CATCAGCCAC ATCAAAGACT CTGTGCTGGA GCTGACAGCC 
AACGTGGAGG GCCCACCCGC CCTGCTGCCC TACTATGTGG CCAACGGCTC CTGGGTGGTG 
CCGCCAGAGC CCCGGCCCAG CTTCTTCTCC ATCTCCCCTC AAGACTGCCA GTTTGCTTTG 
GGGGGCCGCG GCGAGAAGCC AGAGCTGCAG ATGCTGACTG TATCAGAGGA GTTACCGTTT 
GACAACGTGG ATGGCGGCGT GTGGAAGCAA GGCTTCGACA TTTCCTACAG CCCGCACGAC 
TGGGACACCG AAGACCTGCA GGTGTTTGTG GTCCCCCACT CTCACAATGA CCCCGGCTGG 
CTCAAGACCT TTGACAAGTA CTACACAGAA CAGACCCAGC ACATCCTCAA CAACATGGTG 
TCCAAGCTGC AGGAGGACCC CCGGCGGCGC TTCATCTGGG CAGAAGTCTC CTTCTTTGCC 
AAGTGGTGGG ACAACATCAG TGCCCAAAAG AGAGCAGCAG TCCGAAGGCT GGTGGGAAAC 
GGGCAGCTGG AGATTGCAAC AGGAGGCTGG GTGATGCCGG ACGAGGCCAA CTCCCACTAT 
TTTGCATTGA TTGACCAGCT CATTGAGGGC CACCAGTGGC TGGAGAAGAA CCTGGGTGCA 
ACTCCGCGCT CGGGCTGGGC GGTGGACCCC TTTGGATACA GCCCCACCAT GCCTTACCTG 
CTGCGCCGTG CCAACCTGAC CAGCATGCTC ATTCAGAGGG TACACTACGC CATCAAGAAG 
CACTTTGCTG CCAGCCACAG CCTGGAGTTC ATGTGGAGGC AGACCTGGGA CTCGGATGCC 
AGCACAGACA TCTTCTGCCA CATGATGCCT TTCTACAGCT ACGATGTCCC CCATACGTGT 
GGCCCGGATC CCAAGATCTG CTGCCAGTTC GATTTCAAGC GCTTGCCTGG CGGGCGTATC 
AGCTGCCCTT GGAAGGTGCC GCCCCGGGCT ATCACGGAGG CCAATGTGGC TGAGAGGGCA 
GGCCTGCTCC TGGACCAGTA CAGGAAGAAG TCCCGGCTCT TCCGGAGCAA TGTGCTCCTG 
GTGCCGCTGG GCGATGACTT CCGCTACGAC AAGCCCCAGG AGTGGGACGC CCAGTTCTTC 
AACTACCAGC GGCTCTTCGA CTTCCTCAAC AGCAAGCCTG ACCTCCATGT GCAGGACCAC 
TACTGGACAG GCTATTACAC TTCACGGCCT TTCTACAAGA ACCTGGACCG CGTCCTGGAA 
GCACACCTGC GGGGTGCAGA GATTCTGTAC AGCCTGGCCG TGGCTCACGC CCGCCGCGCT 
GGACTGGCCA GCCAGTTCCC ACTCTCAAAC TTCGCCCTTC TGACGGACGC TCGGCGCACA 
CTGGGGCTCT TCCAGCACCA TGACGCAATC ACGGGCACCG CCAAGGAGGC CGTTGTGGTG 
GACTATGGGG TCAGGCTTCT GCGCTCCCTT GTCAGCCTGA AACAGGTCAT CATGAACGCA 
GCTCACTACC TGGTGCTGGG GGACAAGAAG GCCTACCACT TTGACCCAGA GGTGCCCTTC 
CTGCAGATGG ATGACACCCG CTTAAATCAC GACGCCCTTC CAGAGCGCAC AGTGATCCAG 
CTGGAATCCT CACCCAGGTA TGTGGTGCTG TTCAACCCAC TGGAGCAGGA GCGGCTCAGC 
GTGGTGTCCC TGCTGGTGAG CTCACCCCGG GTGCGCGTGC TTTCGGAGGA GGGTCAGCCC 
CTGGCCGTGC AGGTCAGCGC GCACTGGAGC TCTGCCACCG ACATGGTCCC TGACGTCTAT 
CAGGTGTCTG TGCCTGTCCG CCTGCCAGCG CTGGGTCTTG GCGTGCTGCA GCTGCAGCAG 
GGCCTGGATG GGCCCCGCAC GCTGCCCTCC TCTGTGCGCG TCTACCTACA TGGGCGGCCG 
CTGTCCGTCA GCAGGAACGA GGCGTTCCCT CTCCGTGTCA TTGACTCTGG CACCAGTGAC 
TTCGCCCTCA GCAACCGCTA CATGCAGGTC TGGTTCTCAG GCCTTACCGG GCTCCTCAAG 
AGCGTCCGAA GGGTGGATGA GGAGCAGGAA CAGCGGGTGG ACATGGAGTT CCTCGTCTAT 
GGCACCCGCT CGTCCAAAGA CAAGAGTGGA GCCTACCTCT TCCTGCCTGA CGGCGAGGCC 
AAGCCCTACG TCCCCAGGGA CCCACCCGTG CTGCGCGTCA CAGAAGGCCC TTTCTTCTCA 
GAGGTGGTTG CCTGCTACGA GCATGTTCAC CAGGTGGTCC GGCTGTATAA CCTGCCGGGG 
GTGGAGGGGC TGTCCCTGGA TGTGTCGTCC TTGGTGGACA TCCGGGACTA CGTCAACAAG 
GAGCTGGCCC TGCGCATTCG CACTGACATC GATAGCCAGG GCATCTTCTT CACAGACCTC 
AACGGCTTTC AGGTGCAGCC CCGACGGTAT CTGAAGAAGC TGCCCCTGCA GGCCAACTTC 
TACCCCATGC CCGTCATGGC CTACCTCCAG GACGCTCAGA ACCGCCTCAC GCTGCACACA 
GCCCAGGCCC TGGGTGTCTC CAGCCTCCAC AATGGCCAGC TAGAGGTGAT CTTGGACCGG 
AGACTGATGC AGGACGACAA CCGGGGCCTT GGCCAAGGGC TCAAGGACAA CAAGAGAACC 
TGCAACCACT TCCGCCTCCT GTTGGAACGG CGAACCCTGG GCAGGGAGGT CCGCGAGGGC 
TCCTCTGCAA GCTTCCCGTC CCTCCTCAGC CACCTGACCT CCATGTACCT GAACACCCCT 
GTGCTCGCCC TGCCTGTGGC CAGGAGGCAG GCCCCCGGCC CCGCTCTGCG CTCTTTTCAC 
CCTCTGGCCT CCTCACTGCC CTGTGACTTC CACCTGCTCA ACCTGCGGAT GCTCCAGGCC 
GAGGAGGATG GCTTGCCCTC GGCAGAAGCT GCGCTCCTCT TACACCGCAA GGGTTTTGAC 
TGTGGCCTTG AGGCCAAGAA CTTGGGCTTC AACTGCACCA CCAGCCAAGG CAAGGTGGCC 
CTGGGCAGCC TCTTCCATGG CCTGGACGTG GTTTTCCTGC AGCCAACCTC CTTGACCCTG 
CTGTACCCTC TGGCCTCGCC CTCCAATAGC ACTGACGTCT ATGTGGAGCC CATGGAGATC 
GCCACCTTCC GCCTCCGCTT GGGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011988731.1
CDS	437..3763
Translation	MKLKKQVTVCGAAIFCVAVFSLYLMLDRVQHDPARHQNGGNFPRSQISVLQNRIEQLEQLLEENHEIISHIKDSVLELTANVEGPPALLPYYVANGSWVVPPEPRPSFFSISPQDCQFALGGRGEKPELQMLTVSEELPFDNVDGGVWKQGFDISYSPHDWDTEDLQVFVVPHSHNDPGWLKTFDKYYTEQTQHILNNMVSKLQEDPRRRFIWAEVSFFAKWWDNISAQKRAAVRRLVGNGQLEIATGGWVMPDEANSHYFALIDQLIEGHQWLEKNLGATPRSGWAVDPFGYSPTMPYLLRRANLTSMLIQRVHYAIKKHFAASHSLEFMWRQTWDSDASTDIFCHMMPFYSYDVPHTCGPDPKICCQFDFKRLPGGRISCPWKVPPRAITEANVAERAGLLLDQYRKKSRLFRSNVLLVPLGDDFRYDKPQEWDAQFFNYQRLFDFLNSKPDLHVQDHYWTGYYTSRPFYKNLDRVLEAHLRGAEILYSLAVAHARRAGLASQFPLSNFALLTDARRTLGLFQHHDAITGTAKEAVVVDYGVRLLRSLVSLKQVIMNAAHYLVLGDKKAYHFDPEVPFLQMDDTRLNHDALPERTVIQLESSPRYVVLFNPLEQERLSVVSLLVSSPRVRVLSEEGQPLAVQVSAHWSSATDMVPDVYQVSVPVRLPALGLGVLQLQQGLDGPRTLPSSVRVYLHGRPLSVSRNEAFPLRVIDSGTSDFALSNRYMQVWFSGLTGLLKSVRRVDEEQEQRVDMEFLVYGTRSSKDKSGAYLFLPDGEAKPYVPRDPPVLRVTEGPFFSEVVACYEHVHQVVRLYNLPGVEGLSLDVSSLVDIRDYVNKELALRIRTDIDSQGIFFTDLNGFQVQPRRYLKKLPLQANFYPMPVMAYLQDAQNRLTLHTAQALGVSSLHNGQLEVILDRRLMQDDNRGLGQGLKDNKRTCNHFRLLLERRTLGREVREGSSASFPSLLSHLTSMYLNTPVLALPVARRQAPGPALRSFHPLASSLPCDFHLLNLRMLQAEEDGLPSAEAALLLHRKGFDCGLEAKNLGFNCTTSQGKVALGSLFHGLDVVFLQPTSLTLLYPLASPSNSTDVYVEPMEIATFRLRLG

Target ORF information:

RefSeq Version	XM_012133341.2
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon mannosidase, alpha, class 2A, member 2 (MAN2A2), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012133341.2

ORF Insert Sequence:

ATGAAGCTGA AAAAGCAGGT GACAGTGTGT GGGGCTGCTA TCTTCTGCGT GGCTGTCTTC 
TCGCTCTACC TCATGTTGGA CCGAGTACAG CATGATCCTG CTCGACACCA GAACGGTGGG 
AACTTCCCCC GGAGTCAGAT ATCTGTGCTA CAGAACCGCA TTGAACAGCT GGAACAGCTG 
TTGGAGGAGA ACCATGAGAT CATCAGCCAC ATCAAAGACT CTGTGCTGGA GCTGACAGCC 
AACGTGGAGG GCCCACCCGC CCTGCTGCCC TACTATGTGG CCAACGGCTC CTGGGTGGTG 
CCGCCAGAGC CCCGGCCCAG CTTCTTCTCC ATCTCCCCTC AAGACTGCCA GTTTGCTTTG 
GGGGGCCGCG GCGAGAAGCC AGAGCTGCAG ATGCTGACTG TATCAGAGGA GTTACCGTTT 
GACAACGTGG ATGGCGGCGT GTGGAAGCAA GGCTTCGACA TTTCCTACAG CCCGCACGAC 
TGGGACACCG AAGACCTGCA GGTGTTTGTG GTCCCCCACT CTCACAATGA CCCCGGCTGG 
CTCAAGACCT TTGACAAGTA CTACACAGAA CAGACCCAGC ACATCCTCAA CAACATGGTG 
TCCAAGCTGC AGGAGGACCC CCGGCGGCGC TTCATCTGGG CAGAAGTCTC CTTCTTTGCC 
AAGTGGTGGG ACAACATCAG TGCCCAAAAG AGAGCAGCAG TCCGAAGGCT GGTGGGAAAC 
GGGCAGCTGG AGATTGCAAC AGGAGGCTGG GTGATGCCGG ACGAGGCCAA CTCCCACTAT 
TTTGCATTGA TTGACCAGCT CATTGAGGGC CACCAGTGGC TGGAGAAGAA CCTGGGTGCA 
ACTCCGCGCT CGGGCTGGGC GGTGGACCCC TTTGGATACA GCCCCACCAT GCCTTACCTG 
CTGCGCCGTG CCAACCTGAC CAGCATGCTC ATTCAGAGGG TACACTACGC CATCAAGAAG 
CACTTTGCTG CCAGCCACAG CCTGGAGTTC ATGTGGAGGC AGACCTGGGA CTCGGATGCC 
AGCACAGACA TCTTCTGCCA CATGATGCCT TTCTACAGCT ACGATGTCCC CCATACGTGT 
GGCCCGGATC CCAAGATCTG CTGCCAGTTC GATTTCAAGC GCTTGCCTGG CGGGCGTATC 
AGCTGCCCTT GGAAGGTGCC GCCCCGGGCT ATCACGGAGG CCAATGTGGC TGAGAGGGCA 
GGCCTGCTCC TGGACCAGTA CAGGAAGAAG TCCCGGCTCT TCCGGAGCAA TGTGCTCCTG 
GTGCCGCTGG GCGATGACTT CCGCTACGAC AAGCCCCAGG AGTGGGACGC CCAGTTCTTC 
AACTACCAGC GGCTCTTCGA CTTCCTCAAC AGCAAGCCTG ACCTCCATGT GCAGGACCAC 
TACTGGACAG GCTATTACAC TTCACGGCCT TTCTACAAGA ACCTGGACCG CGTCCTGGAA 
GCACACCTGC GGGGTGCAGA GATTCTGTAC AGCCTGGCCG TGGCTCACGC CCGCCGCGCT 
GGACTGGCCA GCCAGTTCCC ACTCTCAAAC TTCGCCCTTC TGACGGACGC TCGGCGCACA 
CTGGGGCTCT TCCAGCACCA TGACGCAATC ACGGGCACCG CCAAGGAGGC CGTTGTGGTG 
GACTATGGGG TCAGGCTTCT GCGCTCCCTT GTCAGCCTGA AACAGGTCAT CATGAACGCA 
GCTCACTACC TGGTGCTGGG GGACAAGAAG GCCTACCACT TTGACCCAGA GGTGCCCTTC 
CTGCAGATGG ATGACACCCG CTTAAATCAC GACGCCCTTC CAGAGCGCAC AGTGATCCAG 
CTGGAATCCT CACCCAGGTA TGTGGTGCTG TTCAACCCAC TGGAGCAGGA GCGGCTCAGC 
GTGGTGTCCC TGCTGGTGAG CTCACCCCGG GTGCGCGTGC TTTCGGAGGA GGGTCAGCCC 
CTGGCCGTGC AGGTCAGCGC GCACTGGAGC TCTGCCACCG ACATGGTCCC TGACGTCTAT 
CAGGTGTCTG TGCCTGTCCG CCTGCCAGCG CTGGGTCTTG GCGTGCTGCA GCTGCAGCAG 
GGCCTGGATG GGCCCCGCAC GCTGCCCTCC TCTGTGCGCG TCTACCTACA TGGGCGGCCG 
CTGTCCGTCA GCAGGAACGA GGCGTTCCCT CTCCGTGTCA TTGACTCTGG CACCAGTGAC 
TTCGCCCTCA GCAACCGCTA CATGCAGGTC TGGTTCTCAG GCCTTACCGG GCTCCTCAAG 
AGCGTCCGAA GGGTGGATGA GGAGCAGGAA CAGCGGGTGG ACATGGAGTT CCTCGTCTAT 
GGCACCCGCT CGTCCAAAGA CAAGAGTGGA GCCTACCTCT TCCTGCCTGA CGGCGAGGCC 
AAGCCCTACG TCCCCAGGGA CCCACCCGTG CTGCGCGTCA CAGAAGGCCC TTTCTTCTCA 
GAGGTGGTTG CCTGCTACGA GCATGTTCAC CAGGTGGTCC GGCTGTATAA CCTGCCGGGG 
GTGGAGGGGC TGTCCCTGGA TGTGTCGTCC TTGGTGGACA TCCGGGACTA CGTCAACAAG 
GAGCTGGCCC TGCGCATTCG CACTGACATC GATAGCCAGG GCATCTTCTT CACAGACCTC 
AACGGCTTTC AGGTGCAGCC CCGACGGTAT CTGAAGAAGC TGCCCCTGCA GGCCAACTTC 
TACCCCATGC CCGTCATGGC CTACCTCCAG GACGCTCAGA ACCGCCTCAC GCTGCACACA 
GCCCAGGCCC TGGGTGTCTC CAGCCTCCAC AATGGCCAGC TAGAGGTGAT CTTGGACCGG 
AGACTGATGC AGGACGACAA CCGGGGCCTT GGCCAAGGGC TCAAGGACAA CAAGAGAACC 
TGCAACCACT TCCGCCTCCT GTTGGAACGG CGAACCCTGG GCAGGGAGGT CCGCGAGGGC 
TCCTCTGCAA GCTTCCCGTC CCTCCTCAGC CACCTGACCT CCATGTACCT GAACACCCCT 
GTGCTCGCCC TGCCTGTGGC CAGGAGGCAG GCCCCCGGCC CCGCTCTGCG CTCTTTTCAC 
CCTCTGGCCT CCTCACTGCC CTGTGACTTC CACCTGCTCA ACCTGCGGAT GCTCCAGGCC 
GAGGAGGATG GCTTGCCCTC GGCAGAAGCT GCGCTCCTCT TACACCGCAA GGGTTTTGAC 
TGTGGCCTTG AGGCCAAGAA CTTGGGCTTC AACTGCACCA CCAGCCAAGG CAAGGTGGCC 
CTGGGCAGCC TCTTCCATGG CCTGGACGTG GTTTTCCTGC AGCCAACCTC CTTGACCCTG 
CTGTACCCTC TGGCCTCGCC CTCCAATAGC ACTGACGTCT ATGTGGAGCC CATGGAGATC 
GCCACCTTCC GCCTCCGCTT GGGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAN2A2 ( XM_015101896.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_014957382.2 Ovis aries mannosidase alpha class 2A member 2 (MAN2A2), transcript variant X1, mRNA.

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CloneID	OSm67701
Clone ID Related Accession (Same CDS sequence)	XM_015101896.2 , XM_027957079.1
Accession Version	XM_015101896.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3528bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-05
Organism	Ovis aries(sheep)
Product	alpha-mannosidase 2x isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040269.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 6, 2019 this sequence version replaced XM_015101896.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ovis aries Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCTGA AAAAGCAGGT GACAGTGTGT GGGGCTGCTA TCTTCTGCGT GGCTGTCTTC 
TCGCTCTACC TCATGTTGGA CCGAGTACAG CATGATCCTG CTCGACACCA GAACGGTGGG 
AACTTCCCCC GGAGTCAGAT ATCTGTGCTA CAGAACCGCA TTGAACAGCT GGAACAGCTG 
TTGGAGGAGA ACCATGAGAT CATCAGCCAC ATCAAAGACT CTGTGCTGGA GCTGACAGCC 
AACGTGGAGG GCCCACCCGC CCTGCTGCCC TACTATGTGG CCAACGGCTC CTGGGTGGTG 
CCGCCAGAGC CCCGGCCCAG CTTCTTCTCC ATCTCCCCTC AAGACTGCCA GTTTGCTTTG 
GGGGGCCGCG GCGAGAAGCC AGAGCTGCAG ATGCTGACTG TATCAGAGGA GTTACCGTTT 
GACAACGTGG ATGGCGGCGT GTGGAAGCAA GGCTTCGACA TTTCCTACAG CCCGCACGAC 
TGGGACACCG AAGACCTGCA GGTGTTTGTG GTCCCCCACT CTCACAATGA CCCCGGCTGG 
CTCAAGACCT TTGACAAGTA CTACACAGAA CAGACCCAGC ACATCCTCAA CAACATGGTG 
TCCAAGCTGC AGGAGGACCC CCGGCGGCGC TTCATCTGGG CAGAAGTCTC CTTCTTTGCC 
AAGTGGTGGG ACAACATCAG TGCCCAAAAG AGAGCAGCAG TCCGAAGGCT GGTGGGAAAC 
GGGCAGCTGG AGATTGCAAC AGGAGGCTGG GTGATGCCGG ACGAGGCCAA CTCCCACTAT 
TTTGCATTGA TTGACCAGCT CATTGAGGGC CACCAGTGGC TGGAGAAGAA CCTGGGTGCA 
ACTCCGCGCT CGGGCTGGGC GGTGGACCCC TTTGGATACA GCCCCACCAT GCCTTACCTG 
CTGCGCCGTG CCAACCTGAC CAGCATGCTC ATTCAGAGGG TACACTACGC CATCAAGAAG 
CACTTTGCTG CCAGCCACAG CCTGGAGTTC ATGTGGAGGC AGACCTGGGA CTCGGATGCC 
AGCACAGACA TCTTCTGCCA CATGATGCCT TTCTACAGCT ACGATGTCCC CCATACGTGT 
GGCCCGGATC CCAAGATCTG CTGCCAGTTC GATTTCAAGC GCTTGCCTGG CGGGCGTATC 
AGCTGCCCTT GGAAGGTGCC GCCCCGGGCT ATCACGGAGG CCAATGTGGC TGAGAGGGCA 
GGCCTGCTCC TGGACCAGTA CAGGAAGAAG TCCCGGCTCT TCCGGAGCAA CGTGCTCCTG 
GTGCCGCTGG GCGATGACTT CCGCTACGAC AAGCCCCAGG AGTGGGACGC CCAGTTCTTC 
AACTACCAGC GGCTCTTCGA CTTCCTCAAC AGCAAGCCTG ACCTCCATGT GCAGGCCCAG 
TTTGGCACCC TCTCCGACTA TTTTGACGCT CTATACAAGA GGACAGGGGT GGAGCCGGGA 
GCCCGGCCTC CAGGTTTCCC TGTGCTGAGT GGGGATTTCT TCTCCTACGC GGACCGGGAG 
GACCACTACT GGACAGGCTA TTACACTTCA CGGCCTTTCT ACAAGAACCT GGACCGCGTC 
CTGGAAGCAC ACCTGCGGGG TGCAGAGATT CTGTACAGCC TGGCCGTGGC TCACGCCCGC 
CGCGCTGGAC TGGCCAGCCA GTTCCCACTC TCAAACTTCG CCCTTCTGAC GGACGCTCGG 
CGCACACTGG GGCTCTTCCA GCACCATGAC GCAATCACGG GCACCGCCAA GGAGGCCGTT 
GTGGTGGACT ATGGGGTCAG GCTTCTGCGC TCCCTTGTCA GCCTGAAACA GGTCATCATG 
AACGCAGCTC ACTACCTGGT GCTGGGGGAC AAGAAGGCCT ACCACTTTGA CCCAGAGGTG 
CCCTTCCTGC AGATGGATGA CACCCGCTTA AATCACGACG CCCTTCCAGA GCGCACAGTG 
ATCCAGCTGG AATCCTCACC CAGGTATGTG GTGCTGTTCA ACCCACTGGA GCAGGAGCGG 
CTCAGCGTGG TGTCCCTGCT GGTGAGCTCA CCCCGGGTGC GCGTGCTTTC GGAGGAGGGT 
CAGCCCCTGG CCGTGCAGGT CAGCGCGCAC TGGAGCTCTG CCACCGACAT GGTCCCTGAC 
GTCTATCAGG TGTCTGTGCC TGTCCGCCTG CCAGCGCTGG GTCTTGGCGT GCTGCAGCTG 
CAGCAGGGCC TGGATGGGCC CCGCACGCTG CCCTCCTCTG TGCGCGTCTA CCTACATGGG 
CGGCCGCTGT CCGTCAGCAG GAACGAGGCG TTCCCTCTCC GTGTCATTGA CTCTGGCACC 
AGTGACTTCG CCCTCAGCAA CCGCTACATG CAGGTCTGGT TCTCAGGCCT TACCGGGCTC 
CTCAAGAGCG TCCGAAGGGT GGATGAGGAG CAGGAACAGC GGGTGGACAT GGAGTTCCTC 
GTCTATGGCA CCCGCTCGTC CAAAGACAAG AGTGGAGCCT ACCTCTTCCT GCCTGACGGC 
GAGGCCAAGC CCTACGTCCC CAGGGACCCA CCCGTGCTGC GCGTCACAGA AGGCCCTTTC 
TTCTCAGAGG TGGTTGCCTG CTACGAGCAT GTTCACCAGG TGGTCCGGCT GTATAACCTG 
CCGGGGGTGG AGGGGCTGTC CCTGGATGTG TCGTCCTTGG TGGACATCCG GGACTACGTC 
AACAAGGAGC TGGCCCTGCG CATTCGCACT GACATCGATA GCCAGGGCAT CTTCTTCACA 
GACCTCAACG GCTTTCAGGT GCAGCCCCGA CGGTATCTGA AGAAGCTGCC CCTGCAGGCC 
AACTTCTACC CCATGCCCGT CATGGCCTAC CTCCAGGACT CTCAGAACCG CCTCACGCTG 
CACACAGCCC AGGCCCTGGG TGTCTCCAGC CTCCACAATG GCCAGCTAGA GGTGATCTTG 
GACCGGAGAC TGATGCAGGA CGACAACCGG GGCCTTGGCC AAGGGCTCAA GGACAACAAG 
AGAACCTGCA ACCACTTCCG CCTCCTGTTG GAACGGCGAA CCCTGGGCAG GGAGCCTGGC 
TTTTTCTCCA AACTGGCAGC CATGTTTAGG GGCTTGATCT TTCACAGCAG CAGGAACAGG 
AACCGAGAGG TCCGCGAGGG CTCCTCTGCA AGCTTCCCGT CCCTCCTCAG CCACCTGACC 
TCCATGTACC TGAACACCCC TGTGCTCGCC CTGCCTGTGG CCAGGAGGCA GGCCCCCGGC 
CCCGCTCTGC GCTCTTTTCA CCCTCTGGCC TCCTCACTGC CCTGTGACTT CCACCTGCTC 
AACCTGCGGA CGCTCCAGGC CGAGGAGGAT GGCTTGCCCT CGGCAGAAGC TGCGCTCCTC 
TTACACCGCA AGGGTTTTGA CTGTGGCCTT GAGGCCAAGA ACTTGGGCTT CAACTGCACC 
ACCAGCCAAG GCAAGGTGGC CCTGGGCAGC CTCTTCCATG GCCTGGACGT GGTTTTCCTG 
CAGCCAACCT CCTTGACCCT GCTGTACCCT CTGGCCTCGC CCTCCAATAG CACTGACGTC 
TATGTGGAGC CCATGGAGAT CGCCACCTTC CGCCTCCGCT TGGGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014957382.2
CDS	415..3942
Translation	MKLKKQVTVCGAAIFCVAVFSLYLMLDRVQHDPARHQNGGNFPRSQISVLQNRIEQLEQLLEENHEIISHIKDSVLELTANVEGPPALLPYYVANGSWVVPPEPRPSFFSISPQDCQFALGGRGEKPELQMLTVSEELPFDNVDGGVWKQGFDISYSPHDWDTEDLQVFVVPHSHNDPGWLKTFDKYYTEQTQHILNNMVSKLQEDPRRRFIWAEVSFFAKWWDNISAQKRAAVRRLVGNGQLEIATGGWVMPDEANSHYFALIDQLIEGHQWLEKNLGATPRSGWAVDPFGYSPTMPYLLRRANLTSMLIQRVHYAIKKHFAASHSLEFMWRQTWDSDASTDIFCHMMPFYSYDVPHTCGPDPKICCQFDFKRLPGGRISCPWKVPPRAITEANVAERAGLLLDQYRKKSRLFRSNVLLVPLGDDFRYDKPQEWDAQFFNYQRLFDFLNSKPDLHVQAQFGTLSDYFDALYKRTGVEPGARPPGFPVLSGDFFSYADREDHYWTGYYTSRPFYKNLDRVLEAHLRGAEILYSLAVAHARRAGLASQFPLSNFALLTDARRTLGLFQHHDAITGTAKEAVVVDYGVRLLRSLVSLKQVIMNAAHYLVLGDKKAYHFDPEVPFLQMDDTRLNHDALPERTVIQLESSPRYVVLFNPLEQERLSVVSLLVSSPRVRVLSEEGQPLAVQVSAHWSSATDMVPDVYQVSVPVRLPALGLGVLQLQQGLDGPRTLPSSVRVYLHGRPLSVSRNEAFPLRVIDSGTSDFALSNRYMQVWFSGLTGLLKSVRRVDEEQEQRVDMEFLVYGTRSSKDKSGAYLFLPDGEAKPYVPRDPPVLRVTEGPFFSEVVACYEHVHQVVRLYNLPGVEGLSLDVSSLVDIRDYVNKELALRIRTDIDSQGIFFTDLNGFQVQPRRYLKKLPLQANFYPMPVMAYLQDSQNRLTLHTAQALGVSSLHNGQLEVILDRRLMQDDNRGLGQGLKDNKRTCNHFRLLLERRTLGREPGFFSKLAAMFRGLIFHSSRNRNREVREGSSASFPSLLSHLTSMYLNTPVLALPVARRQAPGPALRSFHPLASSLPCDFHLLNLRTLQAEEDGLPSAEAALLLHRKGFDCGLEAKNLGFNCTTSQGKVALGSLFHGLDVVFLQPTSLTLLYPLASPSNSTDVYVEPMEIATFRLRLG

Target ORF information:

RefSeq Version	XM_015101896.2
Organism	Ovis aries(sheep)
Definition	Ovis aries mannosidase alpha class 2A member 2 (MAN2A2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015101896.2

ORF Insert Sequence:

ATGAAGCTGA AAAAGCAGGT GACAGTGTGT GGGGCTGCTA TCTTCTGCGT GGCTGTCTTC 
TCGCTCTACC TCATGTTGGA CCGAGTACAG CATGATCCTG CTCGACACCA GAACGGTGGG 
AACTTCCCCC GGAGTCAGAT ATCTGTGCTA CAGAACCGCA TTGAACAGCT GGAACAGCTG 
TTGGAGGAGA ACCATGAGAT CATCAGCCAC ATCAAAGACT CTGTGCTGGA GCTGACAGCC 
AACGTGGAGG GCCCACCCGC CCTGCTGCCC TACTATGTGG CCAACGGCTC CTGGGTGGTG 
CCGCCAGAGC CCCGGCCCAG CTTCTTCTCC ATCTCCCCTC AAGACTGCCA GTTTGCTTTG 
GGGGGCCGCG GCGAGAAGCC AGAGCTGCAG ATGCTGACTG TATCAGAGGA GTTACCGTTT 
GACAACGTGG ATGGCGGCGT GTGGAAGCAA GGCTTCGACA TTTCCTACAG CCCGCACGAC 
TGGGACACCG AAGACCTGCA GGTGTTTGTG GTCCCCCACT CTCACAATGA CCCCGGCTGG 
CTCAAGACCT TTGACAAGTA CTACACAGAA CAGACCCAGC ACATCCTCAA CAACATGGTG 
TCCAAGCTGC AGGAGGACCC CCGGCGGCGC TTCATCTGGG CAGAAGTCTC CTTCTTTGCC 
AAGTGGTGGG ACAACATCAG TGCCCAAAAG AGAGCAGCAG TCCGAAGGCT GGTGGGAAAC 
GGGCAGCTGG AGATTGCAAC AGGAGGCTGG GTGATGCCGG ACGAGGCCAA CTCCCACTAT 
TTTGCATTGA TTGACCAGCT CATTGAGGGC CACCAGTGGC TGGAGAAGAA CCTGGGTGCA 
ACTCCGCGCT CGGGCTGGGC GGTGGACCCC TTTGGATACA GCCCCACCAT GCCTTACCTG 
CTGCGCCGTG CCAACCTGAC CAGCATGCTC ATTCAGAGGG TACACTACGC CATCAAGAAG 
CACTTTGCTG CCAGCCACAG CCTGGAGTTC ATGTGGAGGC AGACCTGGGA CTCGGATGCC 
AGCACAGACA TCTTCTGCCA CATGATGCCT TTCTACAGCT ACGATGTCCC CCATACGTGT 
GGCCCGGATC CCAAGATCTG CTGCCAGTTC GATTTCAAGC GCTTGCCTGG CGGGCGTATC 
AGCTGCCCTT GGAAGGTGCC GCCCCGGGCT ATCACGGAGG CCAATGTGGC TGAGAGGGCA 
GGCCTGCTCC TGGACCAGTA CAGGAAGAAG TCCCGGCTCT TCCGGAGCAA CGTGCTCCTG 
GTGCCGCTGG GCGATGACTT CCGCTACGAC AAGCCCCAGG AGTGGGACGC CCAGTTCTTC 
AACTACCAGC GGCTCTTCGA CTTCCTCAAC AGCAAGCCTG ACCTCCATGT GCAGGCCCAG 
TTTGGCACCC TCTCCGACTA TTTTGACGCT CTATACAAGA GGACAGGGGT GGAGCCGGGA 
GCCCGGCCTC CAGGTTTCCC TGTGCTGAGT GGGGATTTCT TCTCCTACGC GGACCGGGAG 
GACCACTACT GGACAGGCTA TTACACTTCA CGGCCTTTCT ACAAGAACCT GGACCGCGTC 
CTGGAAGCAC ACCTGCGGGG TGCAGAGATT CTGTACAGCC TGGCCGTGGC TCACGCCCGC 
CGCGCTGGAC TGGCCAGCCA GTTCCCACTC TCAAACTTCG CCCTTCTGAC GGACGCTCGG 
CGCACACTGG GGCTCTTCCA GCACCATGAC GCAATCACGG GCACCGCCAA GGAGGCCGTT 
GTGGTGGACT ATGGGGTCAG GCTTCTGCGC TCCCTTGTCA GCCTGAAACA GGTCATCATG 
AACGCAGCTC ACTACCTGGT GCTGGGGGAC AAGAAGGCCT ACCACTTTGA CCCAGAGGTG 
CCCTTCCTGC AGATGGATGA CACCCGCTTA AATCACGACG CCCTTCCAGA GCGCACAGTG 
ATCCAGCTGG AATCCTCACC CAGGTATGTG GTGCTGTTCA ACCCACTGGA GCAGGAGCGG 
CTCAGCGTGG TGTCCCTGCT GGTGAGCTCA CCCCGGGTGC GCGTGCTTTC GGAGGAGGGT 
CAGCCCCTGG CCGTGCAGGT CAGCGCGCAC TGGAGCTCTG CCACCGACAT GGTCCCTGAC 
GTCTATCAGG TGTCTGTGCC TGTCCGCCTG CCAGCGCTGG GTCTTGGCGT GCTGCAGCTG 
CAGCAGGGCC TGGATGGGCC CCGCACGCTG CCCTCCTCTG TGCGCGTCTA CCTACATGGG 
CGGCCGCTGT CCGTCAGCAG GAACGAGGCG TTCCCTCTCC GTGTCATTGA CTCTGGCACC 
AGTGACTTCG CCCTCAGCAA CCGCTACATG CAGGTCTGGT TCTCAGGCCT TACCGGGCTC 
CTCAAGAGCG TCCGAAGGGT GGATGAGGAG CAGGAACAGC GGGTGGACAT GGAGTTCCTC 
GTCTATGGCA CCCGCTCGTC CAAAGACAAG AGTGGAGCCT ACCTCTTCCT GCCTGACGGC 
GAGGCCAAGC CCTACGTCCC CAGGGACCCA CCCGTGCTGC GCGTCACAGA AGGCCCTTTC 
TTCTCAGAGG TGGTTGCCTG CTACGAGCAT GTTCACCAGG TGGTCCGGCT GTATAACCTG 
CCGGGGGTGG AGGGGCTGTC CCTGGATGTG TCGTCCTTGG TGGACATCCG GGACTACGTC 
AACAAGGAGC TGGCCCTGCG CATTCGCACT GACATCGATA GCCAGGGCAT CTTCTTCACA 
GACCTCAACG GCTTTCAGGT GCAGCCCCGA CGGTATCTGA AGAAGCTGCC CCTGCAGGCC 
AACTTCTACC CCATGCCCGT CATGGCCTAC CTCCAGGACT CTCAGAACCG CCTCACGCTG 
CACACAGCCC AGGCCCTGGG TGTCTCCAGC CTCCACAATG GCCAGCTAGA GGTGATCTTG 
GACCGGAGAC TGATGCAGGA CGACAACCGG GGCCTTGGCC AAGGGCTCAA GGACAACAAG 
AGAACCTGCA ACCACTTCCG CCTCCTGTTG GAACGGCGAA CCCTGGGCAG GGAGCCTGGC 
TTTTTCTCCA AACTGGCAGC CATGTTTAGG GGCTTGATCT TTCACAGCAG CAGGAACAGG 
AACCGAGAGG TCCGCGAGGG CTCCTCTGCA AGCTTCCCGT CCCTCCTCAG CCACCTGACC 
TCCATGTACC TGAACACCCC TGTGCTCGCC CTGCCTGTGG CCAGGAGGCA GGCCCCCGGC 
CCCGCTCTGC GCTCTTTTCA CCCTCTGGCC TCCTCACTGC CCTGTGACTT CCACCTGCTC 
AACCTGCGGA CGCTCCAGGC CGAGGAGGAT GGCTTGCCCT CGGCAGAAGC TGCGCTCCTC 
TTACACCGCA AGGGTTTTGA CTGTGGCCTT GAGGCCAAGA ACTTGGGCTT CAACTGCACC 
ACCAGCCAAG GCAAGGTGGC CCTGGGCAGC CTCTTCCATG GCCTGGACGT GGTTTTCCTG 
CAGCCAACCT CCTTGACCCT GCTGTACCCT CTGGCCTCGC CCTCCAATAG CACTGACGTC 
TATGTGGAGC CCATGGAGAT CGCCACCTTC CGCCTCCGCT TGGGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAN2A2 ( XM_027957079.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_027812880.1 Ovis aries mannosidase alpha class 2A member 2 (MAN2A2), transcript variant X2, mRNA.

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CloneID	OSm67701
Clone ID Related Accession (Same CDS sequence)	XM_015101896.2 , XM_027957079.1
Accession Version	XM_027957079.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3528bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-05
Organism	Ovis aries(sheep)
Product	alpha-mannosidase 2x isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040269.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ovis aries Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCTGA AAAAGCAGGT GACAGTGTGT GGGGCTGCTA TCTTCTGCGT GGCTGTCTTC 
TCGCTCTACC TCATGTTGGA CCGAGTACAG CATGATCCTG CTCGACACCA GAACGGTGGG 
AACTTCCCCC GGAGTCAGAT ATCTGTGCTA CAGAACCGCA TTGAACAGCT GGAACAGCTG 
TTGGAGGAGA ACCATGAGAT CATCAGCCAC ATCAAAGACT CTGTGCTGGA GCTGACAGCC 
AACGTGGAGG GCCCACCCGC CCTGCTGCCC TACTATGTGG CCAACGGCTC CTGGGTGGTG 
CCGCCAGAGC CCCGGCCCAG CTTCTTCTCC ATCTCCCCTC AAGACTGCCA GTTTGCTTTG 
GGGGGCCGCG GCGAGAAGCC AGAGCTGCAG ATGCTGACTG TATCAGAGGA GTTACCGTTT 
GACAACGTGG ATGGCGGCGT GTGGAAGCAA GGCTTCGACA TTTCCTACAG CCCGCACGAC 
TGGGACACCG AAGACCTGCA GGTGTTTGTG GTCCCCCACT CTCACAATGA CCCCGGCTGG 
CTCAAGACCT TTGACAAGTA CTACACAGAA CAGACCCAGC ACATCCTCAA CAACATGGTG 
TCCAAGCTGC AGGAGGACCC CCGGCGGCGC TTCATCTGGG CAGAAGTCTC CTTCTTTGCC 
AAGTGGTGGG ACAACATCAG TGCCCAAAAG AGAGCAGCAG TCCGAAGGCT GGTGGGAAAC 
GGGCAGCTGG AGATTGCAAC AGGAGGCTGG GTGATGCCGG ACGAGGCCAA CTCCCACTAT 
TTTGCATTGA TTGACCAGCT CATTGAGGGC CACCAGTGGC TGGAGAAGAA CCTGGGTGCA 
ACTCCGCGCT CGGGCTGGGC GGTGGACCCC TTTGGATACA GCCCCACCAT GCCTTACCTG 
CTGCGCCGTG CCAACCTGAC CAGCATGCTC ATTCAGAGGG TACACTACGC CATCAAGAAG 
CACTTTGCTG CCAGCCACAG CCTGGAGTTC ATGTGGAGGC AGACCTGGGA CTCGGATGCC 
AGCACAGACA TCTTCTGCCA CATGATGCCT TTCTACAGCT ACGATGTCCC CCATACGTGT 
GGCCCGGATC CCAAGATCTG CTGCCAGTTC GATTTCAAGC GCTTGCCTGG CGGGCGTATC 
AGCTGCCCTT GGAAGGTGCC GCCCCGGGCT ATCACGGAGG CCAATGTGGC TGAGAGGGCA 
GGCCTGCTCC TGGACCAGTA CAGGAAGAAG TCCCGGCTCT TCCGGAGCAA CGTGCTCCTG 
GTGCCGCTGG GCGATGACTT CCGCTACGAC AAGCCCCAGG AGTGGGACGC CCAGTTCTTC 
AACTACCAGC GGCTCTTCGA CTTCCTCAAC AGCAAGCCTG ACCTCCATGT GCAGGCCCAG 
TTTGGCACCC TCTCCGACTA TTTTGACGCT CTATACAAGA GGACAGGGGT GGAGCCGGGA 
GCCCGGCCTC CAGGTTTCCC TGTGCTGAGT GGGGATTTCT TCTCCTACGC GGACCGGGAG 
GACCACTACT GGACAGGCTA TTACACTTCA CGGCCTTTCT ACAAGAACCT GGACCGCGTC 
CTGGAAGCAC ACCTGCGGGG TGCAGAGATT CTGTACAGCC TGGCCGTGGC TCACGCCCGC 
CGCGCTGGAC TGGCCAGCCA GTTCCCACTC TCAAACTTCG CCCTTCTGAC GGACGCTCGG 
CGCACACTGG GGCTCTTCCA GCACCATGAC GCAATCACGG GCACCGCCAA GGAGGCCGTT 
GTGGTGGACT ATGGGGTCAG GCTTCTGCGC TCCCTTGTCA GCCTGAAACA GGTCATCATG 
AACGCAGCTC ACTACCTGGT GCTGGGGGAC AAGAAGGCCT ACCACTTTGA CCCAGAGGTG 
CCCTTCCTGC AGATGGATGA CACCCGCTTA AATCACGACG CCCTTCCAGA GCGCACAGTG 
ATCCAGCTGG AATCCTCACC CAGGTATGTG GTGCTGTTCA ACCCACTGGA GCAGGAGCGG 
CTCAGCGTGG TGTCCCTGCT GGTGAGCTCA CCCCGGGTGC GCGTGCTTTC GGAGGAGGGT 
CAGCCCCTGG CCGTGCAGGT CAGCGCGCAC TGGAGCTCTG CCACCGACAT GGTCCCTGAC 
GTCTATCAGG TGTCTGTGCC TGTCCGCCTG CCAGCGCTGG GTCTTGGCGT GCTGCAGCTG 
CAGCAGGGCC TGGATGGGCC CCGCACGCTG CCCTCCTCTG TGCGCGTCTA CCTACATGGG 
CGGCCGCTGT CCGTCAGCAG GAACGAGGCG TTCCCTCTCC GTGTCATTGA CTCTGGCACC 
AGTGACTTCG CCCTCAGCAA CCGCTACATG CAGGTCTGGT TCTCAGGCCT TACCGGGCTC 
CTCAAGAGCG TCCGAAGGGT GGATGAGGAG CAGGAACAGC GGGTGGACAT GGAGTTCCTC 
GTCTATGGCA CCCGCTCGTC CAAAGACAAG AGTGGAGCCT ACCTCTTCCT GCCTGACGGC 
GAGGCCAAGC CCTACGTCCC CAGGGACCCA CCCGTGCTGC GCGTCACAGA AGGCCCTTTC 
TTCTCAGAGG TGGTTGCCTG CTACGAGCAT GTTCACCAGG TGGTCCGGCT GTATAACCTG 
CCGGGGGTGG AGGGGCTGTC CCTGGATGTG TCGTCCTTGG TGGACATCCG GGACTACGTC 
AACAAGGAGC TGGCCCTGCG CATTCGCACT GACATCGATA GCCAGGGCAT CTTCTTCACA 
GACCTCAACG GCTTTCAGGT GCAGCCCCGA CGGTATCTGA AGAAGCTGCC CCTGCAGGCC 
AACTTCTACC CCATGCCCGT CATGGCCTAC CTCCAGGACT CTCAGAACCG CCTCACGCTG 
CACACAGCCC AGGCCCTGGG TGTCTCCAGC CTCCACAATG GCCAGCTAGA GGTGATCTTG 
GACCGGAGAC TGATGCAGGA CGACAACCGG GGCCTTGGCC AAGGGCTCAA GGACAACAAG 
AGAACCTGCA ACCACTTCCG CCTCCTGTTG GAACGGCGAA CCCTGGGCAG GGAGCCTGGC 
TTTTTCTCCA AACTGGCAGC CATGTTTAGG GGCTTGATCT TTCACAGCAG CAGGAACAGG 
AACCGAGAGG TCCGCGAGGG CTCCTCTGCA AGCTTCCCGT CCCTCCTCAG CCACCTGACC 
TCCATGTACC TGAACACCCC TGTGCTCGCC CTGCCTGTGG CCAGGAGGCA GGCCCCCGGC 
CCCGCTCTGC GCTCTTTTCA CCCTCTGGCC TCCTCACTGC CCTGTGACTT CCACCTGCTC 
AACCTGCGGA CGCTCCAGGC CGAGGAGGAT GGCTTGCCCT CGGCAGAAGC TGCGCTCCTC 
TTACACCGCA AGGGTTTTGA CTGTGGCCTT GAGGCCAAGA ACTTGGGCTT CAACTGCACC 
ACCAGCCAAG GCAAGGTGGC CCTGGGCAGC CTCTTCCATG GCCTGGACGT GGTTTTCCTG 
CAGCCAACCT CCTTGACCCT GCTGTACCCT CTGGCCTCGC CCTCCAATAG CACTGACGTC 
TATGTGGAGC CCATGGAGAT CGCCACCTTC CGCCTCCGCT TGGGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_027812880.1
CDS	411..3938
Translation	MKLKKQVTVCGAAIFCVAVFSLYLMLDRVQHDPARHQNGGNFPRSQISVLQNRIEQLEQLLEENHEIISHIKDSVLELTANVEGPPALLPYYVANGSWVVPPEPRPSFFSISPQDCQFALGGRGEKPELQMLTVSEELPFDNVDGGVWKQGFDISYSPHDWDTEDLQVFVVPHSHNDPGWLKTFDKYYTEQTQHILNNMVSKLQEDPRRRFIWAEVSFFAKWWDNISAQKRAAVRRLVGNGQLEIATGGWVMPDEANSHYFALIDQLIEGHQWLEKNLGATPRSGWAVDPFGYSPTMPYLLRRANLTSMLIQRVHYAIKKHFAASHSLEFMWRQTWDSDASTDIFCHMMPFYSYDVPHTCGPDPKICCQFDFKRLPGGRISCPWKVPPRAITEANVAERAGLLLDQYRKKSRLFRSNVLLVPLGDDFRYDKPQEWDAQFFNYQRLFDFLNSKPDLHVQAQFGTLSDYFDALYKRTGVEPGARPPGFPVLSGDFFSYADREDHYWTGYYTSRPFYKNLDRVLEAHLRGAEILYSLAVAHARRAGLASQFPLSNFALLTDARRTLGLFQHHDAITGTAKEAVVVDYGVRLLRSLVSLKQVIMNAAHYLVLGDKKAYHFDPEVPFLQMDDTRLNHDALPERTVIQLESSPRYVVLFNPLEQERLSVVSLLVSSPRVRVLSEEGQPLAVQVSAHWSSATDMVPDVYQVSVPVRLPALGLGVLQLQQGLDGPRTLPSSVRVYLHGRPLSVSRNEAFPLRVIDSGTSDFALSNRYMQVWFSGLTGLLKSVRRVDEEQEQRVDMEFLVYGTRSSKDKSGAYLFLPDGEAKPYVPRDPPVLRVTEGPFFSEVVACYEHVHQVVRLYNLPGVEGLSLDVSSLVDIRDYVNKELALRIRTDIDSQGIFFTDLNGFQVQPRRYLKKLPLQANFYPMPVMAYLQDSQNRLTLHTAQALGVSSLHNGQLEVILDRRLMQDDNRGLGQGLKDNKRTCNHFRLLLERRTLGREPGFFSKLAAMFRGLIFHSSRNRNREVREGSSASFPSLLSHLTSMYLNTPVLALPVARRQAPGPALRSFHPLASSLPCDFHLLNLRTLQAEEDGLPSAEAALLLHRKGFDCGLEAKNLGFNCTTSQGKVALGSLFHGLDVVFLQPTSLTLLYPLASPSNSTDVYVEPMEIATFRLRLG

Target ORF information:

RefSeq Version	XM_027957079.1
Organism	Ovis aries(sheep)
Definition	Ovis aries mannosidase alpha class 2A member 2 (MAN2A2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027957079.1

ORF Insert Sequence:

ATGAAGCTGA AAAAGCAGGT GACAGTGTGT GGGGCTGCTA TCTTCTGCGT GGCTGTCTTC 
TCGCTCTACC TCATGTTGGA CCGAGTACAG CATGATCCTG CTCGACACCA GAACGGTGGG 
AACTTCCCCC GGAGTCAGAT ATCTGTGCTA CAGAACCGCA TTGAACAGCT GGAACAGCTG 
TTGGAGGAGA ACCATGAGAT CATCAGCCAC ATCAAAGACT CTGTGCTGGA GCTGACAGCC 
AACGTGGAGG GCCCACCCGC CCTGCTGCCC TACTATGTGG CCAACGGCTC CTGGGTGGTG 
CCGCCAGAGC CCCGGCCCAG CTTCTTCTCC ATCTCCCCTC AAGACTGCCA GTTTGCTTTG 
GGGGGCCGCG GCGAGAAGCC AGAGCTGCAG ATGCTGACTG TATCAGAGGA GTTACCGTTT 
GACAACGTGG ATGGCGGCGT GTGGAAGCAA GGCTTCGACA TTTCCTACAG CCCGCACGAC 
TGGGACACCG AAGACCTGCA GGTGTTTGTG GTCCCCCACT CTCACAATGA CCCCGGCTGG 
CTCAAGACCT TTGACAAGTA CTACACAGAA CAGACCCAGC ACATCCTCAA CAACATGGTG 
TCCAAGCTGC AGGAGGACCC CCGGCGGCGC TTCATCTGGG CAGAAGTCTC CTTCTTTGCC 
AAGTGGTGGG ACAACATCAG TGCCCAAAAG AGAGCAGCAG TCCGAAGGCT GGTGGGAAAC 
GGGCAGCTGG AGATTGCAAC AGGAGGCTGG GTGATGCCGG ACGAGGCCAA CTCCCACTAT 
TTTGCATTGA TTGACCAGCT CATTGAGGGC CACCAGTGGC TGGAGAAGAA CCTGGGTGCA 
ACTCCGCGCT CGGGCTGGGC GGTGGACCCC TTTGGATACA GCCCCACCAT GCCTTACCTG 
CTGCGCCGTG CCAACCTGAC CAGCATGCTC ATTCAGAGGG TACACTACGC CATCAAGAAG 
CACTTTGCTG CCAGCCACAG CCTGGAGTTC ATGTGGAGGC AGACCTGGGA CTCGGATGCC 
AGCACAGACA TCTTCTGCCA CATGATGCCT TTCTACAGCT ACGATGTCCC CCATACGTGT 
GGCCCGGATC CCAAGATCTG CTGCCAGTTC GATTTCAAGC GCTTGCCTGG CGGGCGTATC 
AGCTGCCCTT GGAAGGTGCC GCCCCGGGCT ATCACGGAGG CCAATGTGGC TGAGAGGGCA 
GGCCTGCTCC TGGACCAGTA CAGGAAGAAG TCCCGGCTCT TCCGGAGCAA CGTGCTCCTG 
GTGCCGCTGG GCGATGACTT CCGCTACGAC AAGCCCCAGG AGTGGGACGC CCAGTTCTTC 
AACTACCAGC GGCTCTTCGA CTTCCTCAAC AGCAAGCCTG ACCTCCATGT GCAGGCCCAG 
TTTGGCACCC TCTCCGACTA TTTTGACGCT CTATACAAGA GGACAGGGGT GGAGCCGGGA 
GCCCGGCCTC CAGGTTTCCC TGTGCTGAGT GGGGATTTCT TCTCCTACGC GGACCGGGAG 
GACCACTACT GGACAGGCTA TTACACTTCA CGGCCTTTCT ACAAGAACCT GGACCGCGTC 
CTGGAAGCAC ACCTGCGGGG TGCAGAGATT CTGTACAGCC TGGCCGTGGC TCACGCCCGC 
CGCGCTGGAC TGGCCAGCCA GTTCCCACTC TCAAACTTCG CCCTTCTGAC GGACGCTCGG 
CGCACACTGG GGCTCTTCCA GCACCATGAC GCAATCACGG GCACCGCCAA GGAGGCCGTT 
GTGGTGGACT ATGGGGTCAG GCTTCTGCGC TCCCTTGTCA GCCTGAAACA GGTCATCATG 
AACGCAGCTC ACTACCTGGT GCTGGGGGAC AAGAAGGCCT ACCACTTTGA CCCAGAGGTG 
CCCTTCCTGC AGATGGATGA CACCCGCTTA AATCACGACG CCCTTCCAGA GCGCACAGTG 
ATCCAGCTGG AATCCTCACC CAGGTATGTG GTGCTGTTCA ACCCACTGGA GCAGGAGCGG 
CTCAGCGTGG TGTCCCTGCT GGTGAGCTCA CCCCGGGTGC GCGTGCTTTC GGAGGAGGGT 
CAGCCCCTGG CCGTGCAGGT CAGCGCGCAC TGGAGCTCTG CCACCGACAT GGTCCCTGAC 
GTCTATCAGG TGTCTGTGCC TGTCCGCCTG CCAGCGCTGG GTCTTGGCGT GCTGCAGCTG 
CAGCAGGGCC TGGATGGGCC CCGCACGCTG CCCTCCTCTG TGCGCGTCTA CCTACATGGG 
CGGCCGCTGT CCGTCAGCAG GAACGAGGCG TTCCCTCTCC GTGTCATTGA CTCTGGCACC 
AGTGACTTCG CCCTCAGCAA CCGCTACATG CAGGTCTGGT TCTCAGGCCT TACCGGGCTC 
CTCAAGAGCG TCCGAAGGGT GGATGAGGAG CAGGAACAGC GGGTGGACAT GGAGTTCCTC 
GTCTATGGCA CCCGCTCGTC CAAAGACAAG AGTGGAGCCT ACCTCTTCCT GCCTGACGGC 
GAGGCCAAGC CCTACGTCCC CAGGGACCCA CCCGTGCTGC GCGTCACAGA AGGCCCTTTC 
TTCTCAGAGG TGGTTGCCTG CTACGAGCAT GTTCACCAGG TGGTCCGGCT GTATAACCTG 
CCGGGGGTGG AGGGGCTGTC CCTGGATGTG TCGTCCTTGG TGGACATCCG GGACTACGTC 
AACAAGGAGC TGGCCCTGCG CATTCGCACT GACATCGATA GCCAGGGCAT CTTCTTCACA 
GACCTCAACG GCTTTCAGGT GCAGCCCCGA CGGTATCTGA AGAAGCTGCC CCTGCAGGCC 
AACTTCTACC CCATGCCCGT CATGGCCTAC CTCCAGGACT CTCAGAACCG CCTCACGCTG 
CACACAGCCC AGGCCCTGGG TGTCTCCAGC CTCCACAATG GCCAGCTAGA GGTGATCTTG 
GACCGGAGAC TGATGCAGGA CGACAACCGG GGCCTTGGCC AAGGGCTCAA GGACAACAAG 
AGAACCTGCA ACCACTTCCG CCTCCTGTTG GAACGGCGAA CCCTGGGCAG GGAGCCTGGC 
TTTTTCTCCA AACTGGCAGC CATGTTTAGG GGCTTGATCT TTCACAGCAG CAGGAACAGG 
AACCGAGAGG TCCGCGAGGG CTCCTCTGCA AGCTTCCCGT CCCTCCTCAG CCACCTGACC 
TCCATGTACC TGAACACCCC TGTGCTCGCC CTGCCTGTGG CCAGGAGGCA GGCCCCCGGC 
CCCGCTCTGC GCTCTTTTCA CCCTCTGGCC TCCTCACTGC CCTGTGACTT CCACCTGCTC 
AACCTGCGGA CGCTCCAGGC CGAGGAGGAT GGCTTGCCCT CGGCAGAAGC TGCGCTCCTC 
TTACACCGCA AGGGTTTTGA CTGTGGCCTT GAGGCCAAGA ACTTGGGCTT CAACTGCACC 
ACCAGCCAAG GCAAGGTGGC CCTGGGCAGC CTCTTCCATG GCCTGGACGT GGTTTTCCTG 
CAGCCAACCT CCTTGACCCT GCTGTACCCT CTGGCCTCGC CCTCCAATAG CACTGACGTC 
TATGTGGAGC CCATGGAGAT CGCCACCTTC CGCCTCCGCT TGGGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAN2A2 ( XM_027957080.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_027812881.1 Ovis aries mannosidase alpha class 2A member 2 (MAN2A2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OSm67702
Clone ID Related Accession (Same CDS sequence)	XM_027957080.1
Accession Version	XM_027957080.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3453bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-05
Organism	Ovis aries(sheep)
Product	alpha-mannosidase 2x isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040269.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ovis aries Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCTGA AAAAGCAGGT GACAGTGTGT GGGGCTGCTA TCTTCTGCGT GGCTGTCTTC 
TCGCTCTACC TCATGTTGGA CCGAGTACAG CATGATCCTG CTCGACACCA GAACGGTGGG 
AACTTCCCCC GGAGTCAGAT ATCTGTGCTA CAGAACCGCA TTGAACAGCT GGAACAGCTG 
TTGGAGGAGA ACCATGAGAT CATCAGCCAC ATCAAAGACT CTGTGCTGGA GCTGACAGCC 
AACGTGGAGG GCCCACCCGC CCTGCTGCCC TACTATGTGG CCAACGGCTC CTGGGTGGTG 
CCGCCAGAGC CCCGGCCCAG CTTCTTCTCC ATCTCCCCTC AAGACTGCCA GTTTGCTTTG 
GGGGGCCGCG GCGAGAAGCC AGAGCTGCAG ATGCTGACTG TATCAGAGGA GTTACCGTTT 
GACAACGTGG ATGGCGGCGT GTGGAAGCAA GGCTTCGACA TTTCCTACAG CCCGCACGAC 
TGGGACACCG AAGACCTGCA GGTGTTTGTG GTCCCCCACT CTCACAATGA CCCCGGCTGG 
CTCAAGACCT TTGACAAGTA CTACACAGAA CAGACCCAGC ACATCCTCAA CAACATGGTG 
TCCAAGCTGC AGGAGGACCC CCGGCGGCGC TTCATCTGGG CAGAAGTCTC CTTCTTTGCC 
AAGTGGTGGG ACAACATCAG TGCCCAAAAG AGAGCAGCAG TCCGAAGGCT GGTGGGAAAC 
GGGCAGCTGG AGATTGCAAC AGGAGGCTGG GTGATGCCGG ACGAGGCCAA CTCCCACTAT 
TTTGCATTGA TTGACCAGCT CATTGAGGGC CACCAGTGGC TGGAGAAGAA CCTGGGTGCA 
ACTCCGCGCT CGGGCTGGGC GGTGGACCCC TTTGGATACA GCCCCACCAT GCCTTACCTG 
CTGCGCCGTG CCAACCTGAC CAGCATGCTC ATTCAGAGGG TACACTACGC CATCAAGAAG 
CACTTTGCTG CCAGCCACAG CCTGGAGTTC ATGTGGAGGC AGACCTGGGA CTCGGATGCC 
AGCACAGACA TCTTCTGCCA CATGATGCCT TTCTACAGCT ACGATGTCCC CCATACGTGT 
GGCCCGGATC CCAAGATCTG CTGCCAGTTC GATTTCAAGC GCTTGCCTGG CGGGCGTATC 
AGCTGCCCTT GGAAGGTGCC GCCCCGGGCT ATCACGGAGG CCAATGTGGC TGAGAGGGCA 
GGCCTGCTCC TGGACCAGTA CAGGAAGAAG TCCCGGCTCT TCCGGAGCAA CGTGCTCCTG 
GTGCCGCTGG GCGATGACTT CCGCTACGAC AAGCCCCAGG AGTGGGACGC CCAGTTCTTC 
AACTACCAGC GGCTCTTCGA CTTCCTCAAC AGCAAGCCTG ACCTCCATGT GCAGGCCCAG 
TTTGGCACCC TCTCCGACTA TTTTGACGCT CTATACAAGA GGACAGGGGT GGAGCCGGGA 
GCCCGGCCTC CAGGTTTCCC TGTGCTGAGT GGGGATTTCT TCTCCTACGC GGACCGGGAG 
GACCACTACT GGACAGGCTA TTACACTTCA CGGCCTTTCT ACAAGAACCT GGACCGCGTC 
CTGGAAGCAC ACCTGCGGGG TGCAGAGATT CTGTACAGCC TGGCCGTGGC TCACGCCCGC 
CGCGCTGGAC TGGCCAGCCA GTTCCCACTC TCAAACTTCG CCCTTCTGAC GGACGCTCGG 
CGCACACTGG GGCTCTTCCA GCACCATGAC GCAATCACGG GCACCGCCAA GGAGGCCGTT 
GTGGTGGACT ATGGGGTCAG GCTTCTGCGC TCCCTTGTCA GCCTGAAACA GGTCATCATG 
AACGCAGCTC ACTACCTGGT GCTGGGGGAC AAGAAGGCCT ACCACTTTGA CCCAGAGGTG 
CCCTTCCTGC AGATGGATGA CACCCGCTTA AATCACGACG CCCTTCCAGA GCGCACAGTG 
ATCCAGCTGG AATCCTCACC CAGGTATGTG GTGCTGTTCA ACCCACTGGA GCAGGAGCGG 
CTCAGCGTGG TGTCCCTGCT GGTGAGCTCA CCCCGGGTGC GCGTGCTTTC GGAGGAGGGT 
CAGCCCCTGG CCGTGCAGGT CAGCGCGCAC TGGAGCTCTG CCACCGACAT GGTCCCTGAC 
GTCTATCAGG TGTCTGTGCC TGTCCGCCTG CCAGCGCTGG GTCTTGGCGT GCTGCAGCTG 
CAGCAGGGCC TGGATGGGCC CCGCACGCTG CCCTCCTCTG TGCGCGTCTA CCTACATGGG 
CGGCCGCTGT CCGTCAGCAG GAACGAGGCG TTCCCTCTCC GTGTCATTGA CTCTGGCACC 
AGTGACTTCG CCCTCAGCAA CCGCTACATG CAGGTCTGGT TCTCAGGCCT TACCGGGCTC 
CTCAAGAGCG TCCGAAGGGT GGATGAGGAG CAGGAACAGC GGGTGGACAT GGAGTTCCTC 
GTCTATGGCA CCCGCTCGTC CAAAGACAAG AGTGGAGCCT ACCTCTTCCT GCCTGACGGC 
GAGGCCAAGC CCTACGTCCC CAGGGACCCA CCCGTGCTGC GCGTCACAGA AGGCCCTTTC 
TTCTCAGAGG TGGTTGCCTG CTACGAGCAT GTTCACCAGG TGGTCCGGCT GTATAACCTG 
CCGGGGGTGG AGGGGCTGTC CCTGGATGTG TCGTCCTTGG TGGACATCCG GGACTACGTC 
AACAAGGAGC TGGCCCTGCG CATTCGCACT GACATCGATA GCCAGGGCAT CTTCTTCACA 
GACCTCAACG GCTTTCAGGT GCAGCCCCGA CGGTATCTGA AGAAGCTGCC CCTGCAGGCC 
AACTTCTACC CCATGCCCGT CATGGCCTAC CTCCAGGACT CTCAGAACCG CCTCACGCTG 
CACACAGCCC AGGCCCTGGG TGTCTCCAGC CTCCACAATG GCCAGCTAGA GGTGATCTTG 
GACCGGAGAC TGATGCAGGA CGACAACCGG GGCCTTGGCC AAGGGCTCAA GGACAACAAG 
AGAACCTGCA ACCACTTCCG CCTCCTGTTG GAACGGCGAA CCCTGGGCAG GGAGGTCCGC 
GAGGGCTCCT CTGCAAGCTT CCCGTCCCTC CTCAGCCACC TGACCTCCAT GTACCTGAAC 
ACCCCTGTGC TCGCCCTGCC TGTGGCCAGG AGGCAGGCCC CCGGCCCCGC TCTGCGCTCT 
TTTCACCCTC TGGCCTCCTC ACTGCCCTGT GACTTCCACC TGCTCAACCT GCGGACGCTC 
CAGGCCGAGG AGGATGGCTT GCCCTCGGCA GAAGCTGCGC TCCTCTTACA CCGCAAGGGT 
TTTGACTGTG GCCTTGAGGC CAAGAACTTG GGCTTCAACT GCACCACCAG CCAAGGCAAG 
GTGGCCCTGG GCAGCCTCTT CCATGGCCTG GACGTGGTTT TCCTGCAGCC AACCTCCTTG 
ACCCTGCTGT ACCCTCTGGC CTCGCCCTCC AATAGCACTG ACGTCTATGT GGAGCCCATG 
GAGATCGCCA CCTTCCGCCT CCGCTTGGGC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_027812881.1
CDS	414..3866
Translation	MKLKKQVTVCGAAIFCVAVFSLYLMLDRVQHDPARHQNGGNFPRSQISVLQNRIEQLEQLLEENHEIISHIKDSVLELTANVEGPPALLPYYVANGSWVVPPEPRPSFFSISPQDCQFALGGRGEKPELQMLTVSEELPFDNVDGGVWKQGFDISYSPHDWDTEDLQVFVVPHSHNDPGWLKTFDKYYTEQTQHILNNMVSKLQEDPRRRFIWAEVSFFAKWWDNISAQKRAAVRRLVGNGQLEIATGGWVMPDEANSHYFALIDQLIEGHQWLEKNLGATPRSGWAVDPFGYSPTMPYLLRRANLTSMLIQRVHYAIKKHFAASHSLEFMWRQTWDSDASTDIFCHMMPFYSYDVPHTCGPDPKICCQFDFKRLPGGRISCPWKVPPRAITEANVAERAGLLLDQYRKKSRLFRSNVLLVPLGDDFRYDKPQEWDAQFFNYQRLFDFLNSKPDLHVQAQFGTLSDYFDALYKRTGVEPGARPPGFPVLSGDFFSYADREDHYWTGYYTSRPFYKNLDRVLEAHLRGAEILYSLAVAHARRAGLASQFPLSNFALLTDARRTLGLFQHHDAITGTAKEAVVVDYGVRLLRSLVSLKQVIMNAAHYLVLGDKKAYHFDPEVPFLQMDDTRLNHDALPERTVIQLESSPRYVVLFNPLEQERLSVVSLLVSSPRVRVLSEEGQPLAVQVSAHWSSATDMVPDVYQVSVPVRLPALGLGVLQLQQGLDGPRTLPSSVRVYLHGRPLSVSRNEAFPLRVIDSGTSDFALSNRYMQVWFSGLTGLLKSVRRVDEEQEQRVDMEFLVYGTRSSKDKSGAYLFLPDGEAKPYVPRDPPVLRVTEGPFFSEVVACYEHVHQVVRLYNLPGVEGLSLDVSSLVDIRDYVNKELALRIRTDIDSQGIFFTDLNGFQVQPRRYLKKLPLQANFYPMPVMAYLQDSQNRLTLHTAQALGVSSLHNGQLEVILDRRLMQDDNRGLGQGLKDNKRTCNHFRLLLERRTLGREVREGSSASFPSLLSHLTSMYLNTPVLALPVARRQAPGPALRSFHPLASSLPCDFHLLNLRTLQAEEDGLPSAEAALLLHRKGFDCGLEAKNLGFNCTTSQGKVALGSLFHGLDVVFLQPTSLTLLYPLASPSNSTDVYVEPMEIATFRLRLG

Target ORF information:

RefSeq Version	XM_027957080.1
Organism	Ovis aries(sheep)
Definition	Ovis aries mannosidase alpha class 2A member 2 (MAN2A2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027957080.1

ORF Insert Sequence:

ATGAAGCTGA AAAAGCAGGT GACAGTGTGT GGGGCTGCTA TCTTCTGCGT GGCTGTCTTC 
TCGCTCTACC TCATGTTGGA CCGAGTACAG CATGATCCTG CTCGACACCA GAACGGTGGG 
AACTTCCCCC GGAGTCAGAT ATCTGTGCTA CAGAACCGCA TTGAACAGCT GGAACAGCTG 
TTGGAGGAGA ACCATGAGAT CATCAGCCAC ATCAAAGACT CTGTGCTGGA GCTGACAGCC 
AACGTGGAGG GCCCACCCGC CCTGCTGCCC TACTATGTGG CCAACGGCTC CTGGGTGGTG 
CCGCCAGAGC CCCGGCCCAG CTTCTTCTCC ATCTCCCCTC AAGACTGCCA GTTTGCTTTG 
GGGGGCCGCG GCGAGAAGCC AGAGCTGCAG ATGCTGACTG TATCAGAGGA GTTACCGTTT 
GACAACGTGG ATGGCGGCGT GTGGAAGCAA GGCTTCGACA TTTCCTACAG CCCGCACGAC 
TGGGACACCG AAGACCTGCA GGTGTTTGTG GTCCCCCACT CTCACAATGA CCCCGGCTGG 
CTCAAGACCT TTGACAAGTA CTACACAGAA CAGACCCAGC ACATCCTCAA CAACATGGTG 
TCCAAGCTGC AGGAGGACCC CCGGCGGCGC TTCATCTGGG CAGAAGTCTC CTTCTTTGCC 
AAGTGGTGGG ACAACATCAG TGCCCAAAAG AGAGCAGCAG TCCGAAGGCT GGTGGGAAAC 
GGGCAGCTGG AGATTGCAAC AGGAGGCTGG GTGATGCCGG ACGAGGCCAA CTCCCACTAT 
TTTGCATTGA TTGACCAGCT CATTGAGGGC CACCAGTGGC TGGAGAAGAA CCTGGGTGCA 
ACTCCGCGCT CGGGCTGGGC GGTGGACCCC TTTGGATACA GCCCCACCAT GCCTTACCTG 
CTGCGCCGTG CCAACCTGAC CAGCATGCTC ATTCAGAGGG TACACTACGC CATCAAGAAG 
CACTTTGCTG CCAGCCACAG CCTGGAGTTC ATGTGGAGGC AGACCTGGGA CTCGGATGCC 
AGCACAGACA TCTTCTGCCA CATGATGCCT TTCTACAGCT ACGATGTCCC CCATACGTGT 
GGCCCGGATC CCAAGATCTG CTGCCAGTTC GATTTCAAGC GCTTGCCTGG CGGGCGTATC 
AGCTGCCCTT GGAAGGTGCC GCCCCGGGCT ATCACGGAGG CCAATGTGGC TGAGAGGGCA 
GGCCTGCTCC TGGACCAGTA CAGGAAGAAG TCCCGGCTCT TCCGGAGCAA CGTGCTCCTG 
GTGCCGCTGG GCGATGACTT CCGCTACGAC AAGCCCCAGG AGTGGGACGC CCAGTTCTTC 
AACTACCAGC GGCTCTTCGA CTTCCTCAAC AGCAAGCCTG ACCTCCATGT GCAGGCCCAG 
TTTGGCACCC TCTCCGACTA TTTTGACGCT CTATACAAGA GGACAGGGGT GGAGCCGGGA 
GCCCGGCCTC CAGGTTTCCC TGTGCTGAGT GGGGATTTCT TCTCCTACGC GGACCGGGAG 
GACCACTACT GGACAGGCTA TTACACTTCA CGGCCTTTCT ACAAGAACCT GGACCGCGTC 
CTGGAAGCAC ACCTGCGGGG TGCAGAGATT CTGTACAGCC TGGCCGTGGC TCACGCCCGC 
CGCGCTGGAC TGGCCAGCCA GTTCCCACTC TCAAACTTCG CCCTTCTGAC GGACGCTCGG 
CGCACACTGG GGCTCTTCCA GCACCATGAC GCAATCACGG GCACCGCCAA GGAGGCCGTT 
GTGGTGGACT ATGGGGTCAG GCTTCTGCGC TCCCTTGTCA GCCTGAAACA GGTCATCATG 
AACGCAGCTC ACTACCTGGT GCTGGGGGAC AAGAAGGCCT ACCACTTTGA CCCAGAGGTG 
CCCTTCCTGC AGATGGATGA CACCCGCTTA AATCACGACG CCCTTCCAGA GCGCACAGTG 
ATCCAGCTGG AATCCTCACC CAGGTATGTG GTGCTGTTCA ACCCACTGGA GCAGGAGCGG 
CTCAGCGTGG TGTCCCTGCT GGTGAGCTCA CCCCGGGTGC GCGTGCTTTC GGAGGAGGGT 
CAGCCCCTGG CCGTGCAGGT CAGCGCGCAC TGGAGCTCTG CCACCGACAT GGTCCCTGAC 
GTCTATCAGG TGTCTGTGCC TGTCCGCCTG CCAGCGCTGG GTCTTGGCGT GCTGCAGCTG 
CAGCAGGGCC TGGATGGGCC CCGCACGCTG CCCTCCTCTG TGCGCGTCTA CCTACATGGG 
CGGCCGCTGT CCGTCAGCAG GAACGAGGCG TTCCCTCTCC GTGTCATTGA CTCTGGCACC 
AGTGACTTCG CCCTCAGCAA CCGCTACATG CAGGTCTGGT TCTCAGGCCT TACCGGGCTC 
CTCAAGAGCG TCCGAAGGGT GGATGAGGAG CAGGAACAGC GGGTGGACAT GGAGTTCCTC 
GTCTATGGCA CCCGCTCGTC CAAAGACAAG AGTGGAGCCT ACCTCTTCCT GCCTGACGGC 
GAGGCCAAGC CCTACGTCCC CAGGGACCCA CCCGTGCTGC GCGTCACAGA AGGCCCTTTC 
TTCTCAGAGG TGGTTGCCTG CTACGAGCAT GTTCACCAGG TGGTCCGGCT GTATAACCTG 
CCGGGGGTGG AGGGGCTGTC CCTGGATGTG TCGTCCTTGG TGGACATCCG GGACTACGTC 
AACAAGGAGC TGGCCCTGCG CATTCGCACT GACATCGATA GCCAGGGCAT CTTCTTCACA 
GACCTCAACG GCTTTCAGGT GCAGCCCCGA CGGTATCTGA AGAAGCTGCC CCTGCAGGCC 
AACTTCTACC CCATGCCCGT CATGGCCTAC CTCCAGGACT CTCAGAACCG CCTCACGCTG 
CACACAGCCC AGGCCCTGGG TGTCTCCAGC CTCCACAATG GCCAGCTAGA GGTGATCTTG 
GACCGGAGAC TGATGCAGGA CGACAACCGG GGCCTTGGCC AAGGGCTCAA GGACAACAAG 
AGAACCTGCA ACCACTTCCG CCTCCTGTTG GAACGGCGAA CCCTGGGCAG GGAGGTCCGC 
GAGGGCTCCT CTGCAAGCTT CCCGTCCCTC CTCAGCCACC TGACCTCCAT GTACCTGAAC 
ACCCCTGTGC TCGCCCTGCC TGTGGCCAGG AGGCAGGCCC CCGGCCCCGC TCTGCGCTCT 
TTTCACCCTC TGGCCTCCTC ACTGCCCTGT GACTTCCACC TGCTCAACCT GCGGACGCTC 
CAGGCCGAGG AGGATGGCTT GCCCTCGGCA GAAGCTGCGC TCCTCTTACA CCGCAAGGGT 
TTTGACTGTG GCCTTGAGGC CAAGAACTTG GGCTTCAACT GCACCACCAG CCAAGGCAAG 
GTGGCCCTGG GCAGCCTCTT CCATGGCCTG GACGTGGTTT TCCTGCAGCC AACCTCCTTG 
ACCCTGCTGT ACCCTCTGGC CTCGCCCTCC AATAGCACTG ACGTCTATGT GGAGCCCATG 
GAGATCGCCA CCTTCCGCCT CCGCTTGGGC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAN2A2 ( XM_027957081.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_027812882.1 Ovis aries mannosidase alpha class 2A member 2 (MAN2A2), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OSm67703
Clone ID Related Accession (Same CDS sequence)	XM_027957081.1
Accession Version	XM_027957081.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3402bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-05
Organism	Ovis aries(sheep)
Product	alpha-mannosidase 2x isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040269.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ovis aries Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCTGA AAAAGCAGGT GACAGTGTGT GGGGCTGCTA TCTTCTGCGT GGCTGTCTTC 
TCGCTCTACC TCATGTTGGA CCGAGTACAG CATGATCCTG CTCGACACCA GAACGGTGGG 
AACTTCCCCC GGAGTCAGAT ATCTGTGCTA CAGAACCGCA TTGAACAGCT GGAACAGCTG 
TTGGAGGAGA ACCATGAGAT CATCAGCCAC ATCAAAGACT CTGTGCTGGA GCTGACAGCC 
AACGTGGAGG GCCCACCCGC CCTGCTGCCC TACTATGTGG CCAACGGCTC CTGGGTGGTG 
CCGCCAGAGC CCCGGCCCAG CTTCTTCTCC ATCTCCCCTC AAGACTGCCA GTTTGCTTTG 
GGGGGCCGCG GCGAGAAGCC AGAGCTGCAG ATGCTGACTG TATCAGAGGA GTTACCGTTT 
GACAACGTGG ATGGCGGCGT GTGGAAGCAA GGCTTCGACA TTTCCTACAG CCCGCACGAC 
TGGGACACCG AAGACCTGCA GGTGTTTGTG GTCCCCCACT CTCACAATGA CCCCGGCTGG 
CTCAAGACCT TTGACAAGTA CTACACAGAA CAGACCCAGC ACATCCTCAA CAACATGGTG 
TCCAAGCTGC AGGAGGACCC CCGGCGGCGC TTCATCTGGG CAGAAGTCTC CTTCTTTGCC 
AAGTGGTGGG ACAACATCAG TGCCCAAAAG AGAGCAGCAG TCCGAAGGCT GGTGGGAAAC 
GGGCAGCTGG AGATTGCAAC AGGAGGCTGG GTGATGCCGG ACGAGGCCAA CTCCCACTAT 
TTTGCATTGA TTGACCAGCT CATTGAGGGC CACCAGTGGC TGGAGAAGAA CCTGGGTGCA 
ACTCCGCGCT CGGGCTGGGC GGTGGACCCC TTTGGATACA GCCCCACCAT GCCTTACCTG 
CTGCGCCGTG CCAACCTGAC CAGCATGCTC ATTCAGAGGG TACACTACGC CATCAAGAAG 
CACTTTGCTG CCAGCCACAG CCTGGAGTTC ATGTGGAGGC AGACCTGGGA CTCGGATGCC 
AGCACAGACA TCTTCTGCCA CATGATGCCT TTCTACAGCT ACGATGTCCC CCATACGTGT 
GGCCCGGATC CCAAGATCTG CTGCCAGTTC GATTTCAAGC GCTTGCCTGG CGGGCGTATC 
AGCTGCCCTT GGAAGGTGCC GCCCCGGGCT ATCACGGAGG CCAATGTGGC TGAGAGGGCA 
GGCCTGCTCC TGGACCAGTA CAGGAAGAAG TCCCGGCTCT TCCGGAGCAA CGTGCTCCTG 
GTGCCGCTGG GCGATGACTT CCGCTACGAC AAGCCCCAGG AGTGGGACGC CCAGTTCTTC 
AACTACCAGC GGCTCTTCGA CTTCCTCAAC AGCAAGCCTG ACCTCCATGT GCAGGACCAC 
TACTGGACAG GCTATTACAC TTCACGGCCT TTCTACAAGA ACCTGGACCG CGTCCTGGAA 
GCACACCTGC GGGGTGCAGA GATTCTGTAC AGCCTGGCCG TGGCTCACGC CCGCCGCGCT 
GGACTGGCCA GCCAGTTCCC ACTCTCAAAC TTCGCCCTTC TGACGGACGC TCGGCGCACA 
CTGGGGCTCT TCCAGCACCA TGACGCAATC ACGGGCACCG CCAAGGAGGC CGTTGTGGTG 
GACTATGGGG TCAGGCTTCT GCGCTCCCTT GTCAGCCTGA AACAGGTCAT CATGAACGCA 
GCTCACTACC TGGTGCTGGG GGACAAGAAG GCCTACCACT TTGACCCAGA GGTGCCCTTC 
CTGCAGATGG ATGACACCCG CTTAAATCAC GACGCCCTTC CAGAGCGCAC AGTGATCCAG 
CTGGAATCCT CACCCAGGTA TGTGGTGCTG TTCAACCCAC TGGAGCAGGA GCGGCTCAGC 
GTGGTGTCCC TGCTGGTGAG CTCACCCCGG GTGCGCGTGC TTTCGGAGGA GGGTCAGCCC 
CTGGCCGTGC AGGTCAGCGC GCACTGGAGC TCTGCCACCG ACATGGTCCC TGACGTCTAT 
CAGGTGTCTG TGCCTGTCCG CCTGCCAGCG CTGGGTCTTG GCGTGCTGCA GCTGCAGCAG 
GGCCTGGATG GGCCCCGCAC GCTGCCCTCC TCTGTGCGCG TCTACCTACA TGGGCGGCCG 
CTGTCCGTCA GCAGGAACGA GGCGTTCCCT CTCCGTGTCA TTGACTCTGG CACCAGTGAC 
TTCGCCCTCA GCAACCGCTA CATGCAGGTC TGGTTCTCAG GCCTTACCGG GCTCCTCAAG 
AGCGTCCGAA GGGTGGATGA GGAGCAGGAA CAGCGGGTGG ACATGGAGTT CCTCGTCTAT 
GGCACCCGCT CGTCCAAAGA CAAGAGTGGA GCCTACCTCT TCCTGCCTGA CGGCGAGGCC 
AAGCCCTACG TCCCCAGGGA CCCACCCGTG CTGCGCGTCA CAGAAGGCCC TTTCTTCTCA 
GAGGTGGTTG CCTGCTACGA GCATGTTCAC CAGGTGGTCC GGCTGTATAA CCTGCCGGGG 
GTGGAGGGGC TGTCCCTGGA TGTGTCGTCC TTGGTGGACA TCCGGGACTA CGTCAACAAG 
GAGCTGGCCC TGCGCATTCG CACTGACATC GATAGCCAGG GCATCTTCTT CACAGACCTC 
AACGGCTTTC AGGTGCAGCC CCGACGGTAT CTGAAGAAGC TGCCCCTGCA GGCCAACTTC 
TACCCCATGC CCGTCATGGC CTACCTCCAG GACTCTCAGA ACCGCCTCAC GCTGCACACA 
GCCCAGGCCC TGGGTGTCTC CAGCCTCCAC AATGGCCAGC TAGAGGTGAT CTTGGACCGG 
AGACTGATGC AGGACGACAA CCGGGGCCTT GGCCAAGGGC TCAAGGACAA CAAGAGAACC 
TGCAACCACT TCCGCCTCCT GTTGGAACGG CGAACCCTGG GCAGGGAGCC TGGCTTTTTC 
TCCAAACTGG CAGCCATGTT TAGGGGCTTG ATCTTTCACA GCAGCAGGAA CAGGAACCGA 
GAGGTCCGCG AGGGCTCCTC TGCAAGCTTC CCGTCCCTCC TCAGCCACCT GACCTCCATG 
TACCTGAACA CCCCTGTGCT CGCCCTGCCT GTGGCCAGGA GGCAGGCCCC CGGCCCCGCT 
CTGCGCTCTT TTCACCCTCT GGCCTCCTCA CTGCCCTGTG ACTTCCACCT GCTCAACCTG 
CGGACGCTCC AGGCCGAGGA GGATGGCTTG CCCTCGGCAG AAGCTGCGCT CCTCTTACAC 
CGCAAGGGTT TTGACTGTGG CCTTGAGGCC AAGAACTTGG GCTTCAACTG CACCACCAGC 
CAAGGCAAGG TGGCCCTGGG CAGCCTCTTC CATGGCCTGG ACGTGGTTTT CCTGCAGCCA 
ACCTCCTTGA CCCTGCTGTA CCCTCTGGCC TCGCCCTCCA ATAGCACTGA CGTCTATGTG 
GAGCCCATGG AGATCGCCAC CTTCCGCCTC CGCTTGGGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_027812882.1
CDS	415..3816
Translation	MKLKKQVTVCGAAIFCVAVFSLYLMLDRVQHDPARHQNGGNFPRSQISVLQNRIEQLEQLLEENHEIISHIKDSVLELTANVEGPPALLPYYVANGSWVVPPEPRPSFFSISPQDCQFALGGRGEKPELQMLTVSEELPFDNVDGGVWKQGFDISYSPHDWDTEDLQVFVVPHSHNDPGWLKTFDKYYTEQTQHILNNMVSKLQEDPRRRFIWAEVSFFAKWWDNISAQKRAAVRRLVGNGQLEIATGGWVMPDEANSHYFALIDQLIEGHQWLEKNLGATPRSGWAVDPFGYSPTMPYLLRRANLTSMLIQRVHYAIKKHFAASHSLEFMWRQTWDSDASTDIFCHMMPFYSYDVPHTCGPDPKICCQFDFKRLPGGRISCPWKVPPRAITEANVAERAGLLLDQYRKKSRLFRSNVLLVPLGDDFRYDKPQEWDAQFFNYQRLFDFLNSKPDLHVQDHYWTGYYTSRPFYKNLDRVLEAHLRGAEILYSLAVAHARRAGLASQFPLSNFALLTDARRTLGLFQHHDAITGTAKEAVVVDYGVRLLRSLVSLKQVIMNAAHYLVLGDKKAYHFDPEVPFLQMDDTRLNHDALPERTVIQLESSPRYVVLFNPLEQERLSVVSLLVSSPRVRVLSEEGQPLAVQVSAHWSSATDMVPDVYQVSVPVRLPALGLGVLQLQQGLDGPRTLPSSVRVYLHGRPLSVSRNEAFPLRVIDSGTSDFALSNRYMQVWFSGLTGLLKSVRRVDEEQEQRVDMEFLVYGTRSSKDKSGAYLFLPDGEAKPYVPRDPPVLRVTEGPFFSEVVACYEHVHQVVRLYNLPGVEGLSLDVSSLVDIRDYVNKELALRIRTDIDSQGIFFTDLNGFQVQPRRYLKKLPLQANFYPMPVMAYLQDSQNRLTLHTAQALGVSSLHNGQLEVILDRRLMQDDNRGLGQGLKDNKRTCNHFRLLLERRTLGREPGFFSKLAAMFRGLIFHSSRNRNREVREGSSASFPSLLSHLTSMYLNTPVLALPVARRQAPGPALRSFHPLASSLPCDFHLLNLRTLQAEEDGLPSAEAALLLHRKGFDCGLEAKNLGFNCTTSQGKVALGSLFHGLDVVFLQPTSLTLLYPLASPSNSTDVYVEPMEIATFRLRLG

Target ORF information:

RefSeq Version	XM_027957081.1
Organism	Ovis aries(sheep)
Definition	Ovis aries mannosidase alpha class 2A member 2 (MAN2A2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027957081.1

ORF Insert Sequence:

ATGAAGCTGA AAAAGCAGGT GACAGTGTGT GGGGCTGCTA TCTTCTGCGT GGCTGTCTTC 
TCGCTCTACC TCATGTTGGA CCGAGTACAG CATGATCCTG CTCGACACCA GAACGGTGGG 
AACTTCCCCC GGAGTCAGAT ATCTGTGCTA CAGAACCGCA TTGAACAGCT GGAACAGCTG 
TTGGAGGAGA ACCATGAGAT CATCAGCCAC ATCAAAGACT CTGTGCTGGA GCTGACAGCC 
AACGTGGAGG GCCCACCCGC CCTGCTGCCC TACTATGTGG CCAACGGCTC CTGGGTGGTG 
CCGCCAGAGC CCCGGCCCAG CTTCTTCTCC ATCTCCCCTC AAGACTGCCA GTTTGCTTTG 
GGGGGCCGCG GCGAGAAGCC AGAGCTGCAG ATGCTGACTG TATCAGAGGA GTTACCGTTT 
GACAACGTGG ATGGCGGCGT GTGGAAGCAA GGCTTCGACA TTTCCTACAG CCCGCACGAC 
TGGGACACCG AAGACCTGCA GGTGTTTGTG GTCCCCCACT CTCACAATGA CCCCGGCTGG 
CTCAAGACCT TTGACAAGTA CTACACAGAA CAGACCCAGC ACATCCTCAA CAACATGGTG 
TCCAAGCTGC AGGAGGACCC CCGGCGGCGC TTCATCTGGG CAGAAGTCTC CTTCTTTGCC 
AAGTGGTGGG ACAACATCAG TGCCCAAAAG AGAGCAGCAG TCCGAAGGCT GGTGGGAAAC 
GGGCAGCTGG AGATTGCAAC AGGAGGCTGG GTGATGCCGG ACGAGGCCAA CTCCCACTAT 
TTTGCATTGA TTGACCAGCT CATTGAGGGC CACCAGTGGC TGGAGAAGAA CCTGGGTGCA 
ACTCCGCGCT CGGGCTGGGC GGTGGACCCC TTTGGATACA GCCCCACCAT GCCTTACCTG 
CTGCGCCGTG CCAACCTGAC CAGCATGCTC ATTCAGAGGG TACACTACGC CATCAAGAAG 
CACTTTGCTG CCAGCCACAG CCTGGAGTTC ATGTGGAGGC AGACCTGGGA CTCGGATGCC 
AGCACAGACA TCTTCTGCCA CATGATGCCT TTCTACAGCT ACGATGTCCC CCATACGTGT 
GGCCCGGATC CCAAGATCTG CTGCCAGTTC GATTTCAAGC GCTTGCCTGG CGGGCGTATC 
AGCTGCCCTT GGAAGGTGCC GCCCCGGGCT ATCACGGAGG CCAATGTGGC TGAGAGGGCA 
GGCCTGCTCC TGGACCAGTA CAGGAAGAAG TCCCGGCTCT TCCGGAGCAA CGTGCTCCTG 
GTGCCGCTGG GCGATGACTT CCGCTACGAC AAGCCCCAGG AGTGGGACGC CCAGTTCTTC 
AACTACCAGC GGCTCTTCGA CTTCCTCAAC AGCAAGCCTG ACCTCCATGT GCAGGACCAC 
TACTGGACAG GCTATTACAC TTCACGGCCT TTCTACAAGA ACCTGGACCG CGTCCTGGAA 
GCACACCTGC GGGGTGCAGA GATTCTGTAC AGCCTGGCCG TGGCTCACGC CCGCCGCGCT 
GGACTGGCCA GCCAGTTCCC ACTCTCAAAC TTCGCCCTTC TGACGGACGC TCGGCGCACA 
CTGGGGCTCT TCCAGCACCA TGACGCAATC ACGGGCACCG CCAAGGAGGC CGTTGTGGTG 
GACTATGGGG TCAGGCTTCT GCGCTCCCTT GTCAGCCTGA AACAGGTCAT CATGAACGCA 
GCTCACTACC TGGTGCTGGG GGACAAGAAG GCCTACCACT TTGACCCAGA GGTGCCCTTC 
CTGCAGATGG ATGACACCCG CTTAAATCAC GACGCCCTTC CAGAGCGCAC AGTGATCCAG 
CTGGAATCCT CACCCAGGTA TGTGGTGCTG TTCAACCCAC TGGAGCAGGA GCGGCTCAGC 
GTGGTGTCCC TGCTGGTGAG CTCACCCCGG GTGCGCGTGC TTTCGGAGGA GGGTCAGCCC 
CTGGCCGTGC AGGTCAGCGC GCACTGGAGC TCTGCCACCG ACATGGTCCC TGACGTCTAT 
CAGGTGTCTG TGCCTGTCCG CCTGCCAGCG CTGGGTCTTG GCGTGCTGCA GCTGCAGCAG 
GGCCTGGATG GGCCCCGCAC GCTGCCCTCC TCTGTGCGCG TCTACCTACA TGGGCGGCCG 
CTGTCCGTCA GCAGGAACGA GGCGTTCCCT CTCCGTGTCA TTGACTCTGG CACCAGTGAC 
TTCGCCCTCA GCAACCGCTA CATGCAGGTC TGGTTCTCAG GCCTTACCGG GCTCCTCAAG 
AGCGTCCGAA GGGTGGATGA GGAGCAGGAA CAGCGGGTGG ACATGGAGTT CCTCGTCTAT 
GGCACCCGCT CGTCCAAAGA CAAGAGTGGA GCCTACCTCT TCCTGCCTGA CGGCGAGGCC 
AAGCCCTACG TCCCCAGGGA CCCACCCGTG CTGCGCGTCA CAGAAGGCCC TTTCTTCTCA 
GAGGTGGTTG CCTGCTACGA GCATGTTCAC CAGGTGGTCC GGCTGTATAA CCTGCCGGGG 
GTGGAGGGGC TGTCCCTGGA TGTGTCGTCC TTGGTGGACA TCCGGGACTA CGTCAACAAG 
GAGCTGGCCC TGCGCATTCG CACTGACATC GATAGCCAGG GCATCTTCTT CACAGACCTC 
AACGGCTTTC AGGTGCAGCC CCGACGGTAT CTGAAGAAGC TGCCCCTGCA GGCCAACTTC 
TACCCCATGC CCGTCATGGC CTACCTCCAG GACTCTCAGA ACCGCCTCAC GCTGCACACA 
GCCCAGGCCC TGGGTGTCTC CAGCCTCCAC AATGGCCAGC TAGAGGTGAT CTTGGACCGG 
AGACTGATGC AGGACGACAA CCGGGGCCTT GGCCAAGGGC TCAAGGACAA CAAGAGAACC 
TGCAACCACT TCCGCCTCCT GTTGGAACGG CGAACCCTGG GCAGGGAGCC TGGCTTTTTC 
TCCAAACTGG CAGCCATGTT TAGGGGCTTG ATCTTTCACA GCAGCAGGAA CAGGAACCGA 
GAGGTCCGCG AGGGCTCCTC TGCAAGCTTC CCGTCCCTCC TCAGCCACCT GACCTCCATG 
TACCTGAACA CCCCTGTGCT CGCCCTGCCT GTGGCCAGGA GGCAGGCCCC CGGCCCCGCT 
CTGCGCTCTT TTCACCCTCT GGCCTCCTCA CTGCCCTGTG ACTTCCACCT GCTCAACCTG 
CGGACGCTCC AGGCCGAGGA GGATGGCTTG CCCTCGGCAG AAGCTGCGCT CCTCTTACAC 
CGCAAGGGTT TTGACTGTGG CCTTGAGGCC AAGAACTTGG GCTTCAACTG CACCACCAGC 
CAAGGCAAGG TGGCCCTGGG CAGCCTCTTC CATGGCCTGG ACGTGGTTTT CCTGCAGCCA 
ACCTCCTTGA CCCTGCTGTA CCCTCTGGCC TCGCCCTCCA ATAGCACTGA CGTCTATGTG 
GAGCCCATGG AGATCGCCAC CTTCCGCCTC CGCTTGGGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAN2A2 ( XM_004017759.3 ) cDNA ORF clone, Ovis aries(sheep) -> XP_004017808.1 Ovis aries mannosidase, alpha, class 2A, member 2 (MAN2A2), transcript variant X4, mRNA.

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CloneID	OSm14138
Clone ID Related Accession (Same CDS sequence)	XM_004017759.3
Accession Version	XM_004017759.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3453bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries(sheep)
Product	alpha-mannosidase 2x isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014639027.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_004017759.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCTGA AAAAGCAGGT GACAGTGTGT GGGGCTGCTA TCTTCTGCGT GGCTGTCTTC 
TCGCTCTACC TCATGTTGGA CCGAGTACAG CATGATCCTG CTCGACACCA GAACGGTGGG 
AACTTCCCCC GGAGTCAGAT ATCTGTGCTA CAGAACCGCA TTGAACAGCT GGAACAGCTG 
TTGGAGGAGA ACCATGAGAT CATCAGCCAC ATCAAAGACT CTGTGCTGGA GCTGACAGCC 
AACGTGGAGG GCCCACCCGC CCTGCTGCCC TACTATGTGG CCAACGGCTC CTGGGTGGTG 
CCGCCAGAGC CCCGGCCCAG CTTCTTCTCC ATCTCCCCTC AAGACTGCCA GTTTGCTTTG 
GGGGGCCGCG GCGAGAAGCC AGAGCTGCAG ATGCTGACTG TATCAGAGGA GTTACCGTTT 
GACAACGTGG ATGGCGGCGT GTGGAAGCAA GGCTTCGACA TTTCCTACAG CCCGCACGAC 
TGGGACACCG AAGACCTGCA GGTGTTTGTG GTCCCCCACT CTCACAATGA CCCCGGCTGG 
CTCAAGACCT TTGACAAGTA CTACACAGAA CAGACCCAGC ACATCCTCAA CAACATGGTG 
TCCAAGCTGC AGGAGGACCC CCGGCGGCGC TTCATCTGGG CAGAAGTCTC CTTCTTTGCC 
AAGTGGTGGG ACAACATCAG TGCCCAAAAG AGAGCAGCAG TCCGAAGGCT GGTGGGAAAC 
GGGCAGCTGG AGATTGCAAC AGGAGGCTGG GTGATGCCGG ACGAGGCCAA CTCCCACTAT 
TTTGCATTGA TTGACCAGCT CATTGAGGGC CACCAGTGGC TGGAGAAGAA CCTGGGTGCA 
ACTCCGCGCT CGGGCTGGGC GGTGGACCCC TTTGGATACA GCCCCACCAT GCCTTACCTG 
CTGCGCCGTG CCAACCTGAC CAGCATGCTC ATTCAGAGGG TACACTACGC CATCAAGAAG 
CACTTTGCTG CCAGCCACAG CCTGGAGTTC ATGTGGAGGC AGACCTGGGA CTCGGATGCC 
AGCACAGACA TCTTCTGCCA CATGATGCCT TTCTACAGCT ACGATGTCCC CCATACGTGT 
GGCCCGGATC CCAAGATCTG CTGCCAGTTC GATTTCAAGC GCTTGCCTGG CGGGCGTATC 
AGCTGCCCTT GGAAGGTGCC GCCCCGGGCT ATCACGGAGG CCAATGTGGC TGAGAGGGCA 
GGCCTGCTCC TGGACCAGTA CAGGAAGAAG TCCCGGCTCT TCCGGAGCAA TGTGCTCCTG 
GTGCCGCTGG GCGATGACTT CCGCTACGAC AAGCCCCAGG AGTGGGACGC CCAGTTCTTC 
AACTACCAGC GGCTCTTCGA CTTCCTCAAC AGCAAGCCTG ACCTCCATGT GCAGGCCCAG 
TTTGGCACCC TCTCCGACTA TTTTGACGCT CTATACAAGA GGACAGGGGT GGAGCCGGGA 
GCCCGGCCTC CAGGTTTCCC TGTGCTGAGT GGGGATTTCT TCTCCTACGC GGACCGGGAG 
GACCACTACT GGACAGGCTA TTACACTTCA CGGCCTTTCT ACAAGAACCT GGACCGCGTC 
CTGGAAGCAC ACCTGCGGGG TGCAGAGATT CTGTACAGCC TGGCCGTGGC TCACGCCCGC 
CGCGCTGGAC TGGCCAGCCA GTTCCCACTC TCAAACTTCG CCCTTCTGAC GGACGCTCGG 
CGCACACTGG GGCTCTTCCA GCACCATGAC GCAATCACGG GCACCGCCAA GGAGGCCGTT 
GTGGTGGACT ATGGGGTCAG GCTTCTGCGC TCCCTTGTCA GCCTGAAACA GGTCATCATG 
AACGCAGCTC ACTACCTGGT GCTGGGGGAC AAGAAGGCCT ACCACTTTGA CCCAGAGGTG 
CCCTTCCTGC AGATGGATGA CACCCGCTTA AATCACGACG CCCTTCCAGA GCGCACAGTG 
ATCCAGCTGG AATCCTCACC CAGGTATGTG GTGCTGTTCA ACCCACTGGA GCAGGAGCGG 
CTCAGCGTGG TGTCCCTGCT GGTGAGCTCA CCCCGGGTGC GCGTGCTTTC GGAGGAGGGT 
CAGCCCCTGG CCGTGCAGGT CAGCGCGCAC TGGAGCTCTG CCACCGACAT GGTCCCTGAT 
GTCTATCAGG TGTCTGTGCC TGTCCGCCTG CCAGCGCTGG GTCTTGGCGT GCTGCAGCTG 
CAGCAGGGCC TGGATGGGCC CCGCACGCTG CCCTCCTCTG TGCGCGTCTA CCTACATGGG 
CGGCCGCTGT CCGTCAGCAG GAACGAGGCG TTCCCTCTCC GTGTCATTGA CTCTGGCACC 
AGTGACTTCG CCCTCAGCAA CCGCTACATG CAGGTCTGGT TCTCAGGCCT TACCGGGCTC 
CTCAAGAGCG TCCGAAGGGT GGATGAGGAG CAGGAACAGC GGGTGGACAT GGAGTTCCTC 
GTCTATGGCA CCCGCTCGTC CAAAGACAAG AGTGGAGCCT ACCTCTTCCT GCCTGACGGC 
GAGGCCAAGC CCTACGTCCC CAGGGACCCA CCCGTGCTGC GCGTCACAGA AGGCCCTTTC 
TTCTCAGAGG TGGTTGCCTG CTACGAGCAT GTTCACCAGG TGGTCCGGCT GTATAACCTG 
CCGGGGGTGG AGGGGCTGTC CCTGGATGTG TCGTCCTTGG TGGACATCCG GGACTACGTC 
AACAAGGAGC TGGCCCTGCG CATTCGCACT GACATCGATA GCCAGGGCAT CTTCTTCACA 
GACCTCAACG GCTTTCAGGT GCAGCCCCGA CGGTATCTGA AGAAGCTGCC CCTGCAGGCC 
AACTTCTACC CCATGCCCGT CATGGCCTAC CTCCAGGACG CTCAGAACCG CCTCACGCTG 
CACACAGCCC AGGCCCTGGG TGTCTCCAGC CTCCACAATG GCCAGCTAGA GGTGATCTTG 
GACCGGAGAC TGATGCAGGA CGACAACCGG GGCCTTGGCC AAGGGCTCAA GGACAACAAG 
AGAACCTGCA ACCACTTCCG CCTCCTGTTG GAACGGCGAA CCCTGGGCAG GGAGGTCCGC 
GAGGGCTCCT CTGCAAGCTT CCCGTCCCTC CTCAGCCACC TGACCTCCAT GTACCTGAAC 
ACCCCTGTGC TCGCCCTGCC TGTGGCCAGG AGGCAGGCCC CCGGCCCCGC TCTGCGCTCT 
TTTCACCCTC TGGCCTCCTC ACTGCCCTGT GACTTCCACC TGCTCAACCT GCGGACGCTC 
CAGGCCGAGG AGGATGGCTT GCCCTCGGCA GAAGCTGCGC TCCTCTTACA CCGCAAGGGT 
TTTGACTGTG GCCTTGAGGC CAAGAACTTG GGCTTCAACT GCACCACCAG CCAAGGCAAG 
GTGGCCCTGG GCAGCCTCTT CCATGGCCTG GACGTGGTTT TCCTGCAGCC AACCTCCTTG 
ACCCTGCTGT ACCCTCTGGC CTCGCCCTCC AATAGCACTG ACGTCTATGT GGAGCCCATG 
GAGATCGCCA CCTTCCGCCT CCGCTTGGGC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004017808.1
CDS	394..3846
Translation	MKLKKQVTVCGAAIFCVAVFSLYLMLDRVQHDPARHQNGGNFPRSQISVLQNRIEQLEQLLEENHEIISHIKDSVLELTANVEGPPALLPYYVANGSWVVPPEPRPSFFSISPQDCQFALGGRGEKPELQMLTVSEELPFDNVDGGVWKQGFDISYSPHDWDTEDLQVFVVPHSHNDPGWLKTFDKYYTEQTQHILNNMVSKLQEDPRRRFIWAEVSFFAKWWDNISAQKRAAVRRLVGNGQLEIATGGWVMPDEANSHYFALIDQLIEGHQWLEKNLGATPRSGWAVDPFGYSPTMPYLLRRANLTSMLIQRVHYAIKKHFAASHSLEFMWRQTWDSDASTDIFCHMMPFYSYDVPHTCGPDPKICCQFDFKRLPGGRISCPWKVPPRAITEANVAERAGLLLDQYRKKSRLFRSNVLLVPLGDDFRYDKPQEWDAQFFNYQRLFDFLNSKPDLHVQAQFGTLSDYFDALYKRTGVEPGARPPGFPVLSGDFFSYADREDHYWTGYYTSRPFYKNLDRVLEAHLRGAEILYSLAVAHARRAGLASQFPLSNFALLTDARRTLGLFQHHDAITGTAKEAVVVDYGVRLLRSLVSLKQVIMNAAHYLVLGDKKAYHFDPEVPFLQMDDTRLNHDALPERTVIQLESSPRYVVLFNPLEQERLSVVSLLVSSPRVRVLSEEGQPLAVQVSAHWSSATDMVPDVYQVSVPVRLPALGLGVLQLQQGLDGPRTLPSSVRVYLHGRPLSVSRNEAFPLRVIDSGTSDFALSNRYMQVWFSGLTGLLKSVRRVDEEQEQRVDMEFLVYGTRSSKDKSGAYLFLPDGEAKPYVPRDPPVLRVTEGPFFSEVVACYEHVHQVVRLYNLPGVEGLSLDVSSLVDIRDYVNKELALRIRTDIDSQGIFFTDLNGFQVQPRRYLKKLPLQANFYPMPVMAYLQDAQNRLTLHTAQALGVSSLHNGQLEVILDRRLMQDDNRGLGQGLKDNKRTCNHFRLLLERRTLGREVREGSSASFPSLLSHLTSMYLNTPVLALPVARRQAPGPALRSFHPLASSLPCDFHLLNLRTLQAEEDGLPSAEAALLLHRKGFDCGLEAKNLGFNCTTSQGKVALGSLFHGLDVVFLQPTSLTLLYPLASPSNSTDVYVEPMEIATFRLRLG

Target ORF information:

RefSeq Version	XM_004017759.3
Organism	Ovis aries(sheep)
Definition	Ovis aries mannosidase, alpha, class 2A, member 2 (MAN2A2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004017759.3

ORF Insert Sequence:

ATGAAGCTGA AAAAGCAGGT GACAGTGTGT GGGGCTGCTA TCTTCTGCGT GGCTGTCTTC 
TCGCTCTACC TCATGTTGGA CCGAGTACAG CATGATCCTG CTCGACACCA GAACGGTGGG 
AACTTCCCCC GGAGTCAGAT ATCTGTGCTA CAGAACCGCA TTGAACAGCT GGAACAGCTG 
TTGGAGGAGA ACCATGAGAT CATCAGCCAC ATCAAAGACT CTGTGCTGGA GCTGACAGCC 
AACGTGGAGG GCCCACCCGC CCTGCTGCCC TACTATGTGG CCAACGGCTC CTGGGTGGTG 
CCGCCAGAGC CCCGGCCCAG CTTCTTCTCC ATCTCCCCTC AAGACTGCCA GTTTGCTTTG 
GGGGGCCGCG GCGAGAAGCC AGAGCTGCAG ATGCTGACTG TATCAGAGGA GTTACCGTTT 
GACAACGTGG ATGGCGGCGT GTGGAAGCAA GGCTTCGACA TTTCCTACAG CCCGCACGAC 
TGGGACACCG AAGACCTGCA GGTGTTTGTG GTCCCCCACT CTCACAATGA CCCCGGCTGG 
CTCAAGACCT TTGACAAGTA CTACACAGAA CAGACCCAGC ACATCCTCAA CAACATGGTG 
TCCAAGCTGC AGGAGGACCC CCGGCGGCGC TTCATCTGGG CAGAAGTCTC CTTCTTTGCC 
AAGTGGTGGG ACAACATCAG TGCCCAAAAG AGAGCAGCAG TCCGAAGGCT GGTGGGAAAC 
GGGCAGCTGG AGATTGCAAC AGGAGGCTGG GTGATGCCGG ACGAGGCCAA CTCCCACTAT 
TTTGCATTGA TTGACCAGCT CATTGAGGGC CACCAGTGGC TGGAGAAGAA CCTGGGTGCA 
ACTCCGCGCT CGGGCTGGGC GGTGGACCCC TTTGGATACA GCCCCACCAT GCCTTACCTG 
CTGCGCCGTG CCAACCTGAC CAGCATGCTC ATTCAGAGGG TACACTACGC CATCAAGAAG 
CACTTTGCTG CCAGCCACAG CCTGGAGTTC ATGTGGAGGC AGACCTGGGA CTCGGATGCC 
AGCACAGACA TCTTCTGCCA CATGATGCCT TTCTACAGCT ACGATGTCCC CCATACGTGT 
GGCCCGGATC CCAAGATCTG CTGCCAGTTC GATTTCAAGC GCTTGCCTGG CGGGCGTATC 
AGCTGCCCTT GGAAGGTGCC GCCCCGGGCT ATCACGGAGG CCAATGTGGC TGAGAGGGCA 
GGCCTGCTCC TGGACCAGTA CAGGAAGAAG TCCCGGCTCT TCCGGAGCAA TGTGCTCCTG 
GTGCCGCTGG GCGATGACTT CCGCTACGAC AAGCCCCAGG AGTGGGACGC CCAGTTCTTC 
AACTACCAGC GGCTCTTCGA CTTCCTCAAC AGCAAGCCTG ACCTCCATGT GCAGGCCCAG 
TTTGGCACCC TCTCCGACTA TTTTGACGCT CTATACAAGA GGACAGGGGT GGAGCCGGGA 
GCCCGGCCTC CAGGTTTCCC TGTGCTGAGT GGGGATTTCT TCTCCTACGC GGACCGGGAG 
GACCACTACT GGACAGGCTA TTACACTTCA CGGCCTTTCT ACAAGAACCT GGACCGCGTC 
CTGGAAGCAC ACCTGCGGGG TGCAGAGATT CTGTACAGCC TGGCCGTGGC TCACGCCCGC 
CGCGCTGGAC TGGCCAGCCA GTTCCCACTC TCAAACTTCG CCCTTCTGAC GGACGCTCGG 
CGCACACTGG GGCTCTTCCA GCACCATGAC GCAATCACGG GCACCGCCAA GGAGGCCGTT 
GTGGTGGACT ATGGGGTCAG GCTTCTGCGC TCCCTTGTCA GCCTGAAACA GGTCATCATG 
AACGCAGCTC ACTACCTGGT GCTGGGGGAC AAGAAGGCCT ACCACTTTGA CCCAGAGGTG 
CCCTTCCTGC AGATGGATGA CACCCGCTTA AATCACGACG CCCTTCCAGA GCGCACAGTG 
ATCCAGCTGG AATCCTCACC CAGGTATGTG GTGCTGTTCA ACCCACTGGA GCAGGAGCGG 
CTCAGCGTGG TGTCCCTGCT GGTGAGCTCA CCCCGGGTGC GCGTGCTTTC GGAGGAGGGT 
CAGCCCCTGG CCGTGCAGGT CAGCGCGCAC TGGAGCTCTG CCACCGACAT GGTCCCTGAT 
GTCTATCAGG TGTCTGTGCC TGTCCGCCTG CCAGCGCTGG GTCTTGGCGT GCTGCAGCTG 
CAGCAGGGCC TGGATGGGCC CCGCACGCTG CCCTCCTCTG TGCGCGTCTA CCTACATGGG 
CGGCCGCTGT CCGTCAGCAG GAACGAGGCG TTCCCTCTCC GTGTCATTGA CTCTGGCACC 
AGTGACTTCG CCCTCAGCAA CCGCTACATG CAGGTCTGGT TCTCAGGCCT TACCGGGCTC 
CTCAAGAGCG TCCGAAGGGT GGATGAGGAG CAGGAACAGC GGGTGGACAT GGAGTTCCTC 
GTCTATGGCA CCCGCTCGTC CAAAGACAAG AGTGGAGCCT ACCTCTTCCT GCCTGACGGC 
GAGGCCAAGC CCTACGTCCC CAGGGACCCA CCCGTGCTGC GCGTCACAGA AGGCCCTTTC 
TTCTCAGAGG TGGTTGCCTG CTACGAGCAT GTTCACCAGG TGGTCCGGCT GTATAACCTG 
CCGGGGGTGG AGGGGCTGTC CCTGGATGTG TCGTCCTTGG TGGACATCCG GGACTACGTC 
AACAAGGAGC TGGCCCTGCG CATTCGCACT GACATCGATA GCCAGGGCAT CTTCTTCACA 
GACCTCAACG GCTTTCAGGT GCAGCCCCGA CGGTATCTGA AGAAGCTGCC CCTGCAGGCC 
AACTTCTACC CCATGCCCGT CATGGCCTAC CTCCAGGACG CTCAGAACCG CCTCACGCTG 
CACACAGCCC AGGCCCTGGG TGTCTCCAGC CTCCACAATG GCCAGCTAGA GGTGATCTTG 
GACCGGAGAC TGATGCAGGA CGACAACCGG GGCCTTGGCC AAGGGCTCAA GGACAACAAG 
AGAACCTGCA ACCACTTCCG CCTCCTGTTG GAACGGCGAA CCCTGGGCAG GGAGGTCCGC 
GAGGGCTCCT CTGCAAGCTT CCCGTCCCTC CTCAGCCACC TGACCTCCAT GTACCTGAAC 
ACCCCTGTGC TCGCCCTGCC TGTGGCCAGG AGGCAGGCCC CCGGCCCCGC TCTGCGCTCT 
TTTCACCCTC TGGCCTCCTC ACTGCCCTGT GACTTCCACC TGCTCAACCT GCGGACGCTC 
CAGGCCGAGG AGGATGGCTT GCCCTCGGCA GAAGCTGCGC TCCTCTTACA CCGCAAGGGT 
TTTGACTGTG GCCTTGAGGC CAAGAACTTG GGCTTCAACT GCACCACCAG CCAAGGCAAG 
GTGGCCCTGG GCAGCCTCTT CCATGGCCTG GACGTGGTTT TCCTGCAGCC AACCTCCTTG 
ACCCTGCTGT ACCCTCTGGC CTCGCCCTCC AATAGCACTG ACGTCTATGT GGAGCCCATG 
GAGATCGCCA CCTTCCGCCT CCGCTTGGGC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAN2A2 ( XM_012098482.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_011953872.1 Ovis aries mannosidase, alpha, class 2A, member 2 (MAN2A2), transcript variant X1, mRNA.

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CloneID	OSm27643
Clone ID Related Accession (Same CDS sequence)	XM_012098482.2 , XM_015101896.1 , XM_012098481.2 , XM_012098483.1
Accession Version	XM_012098482.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3528bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries(sheep)
Product	alpha-mannosidase 2x isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014639027.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012098482.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCTGA AAAAGCAGGT GACAGTGTGT GGGGCTGCTA TCTTCTGCGT GGCTGTCTTC 
TCGCTCTACC TCATGTTGGA CCGAGTACAG CATGATCCTG CTCGACACCA GAACGGTGGG 
AACTTCCCCC GGAGTCAGAT ATCTGTGCTA CAGAACCGCA TTGAACAGCT GGAACAGCTG 
TTGGAGGAGA ACCATGAGAT CATCAGCCAC ATCAAAGACT CTGTGCTGGA GCTGACAGCC 
AACGTGGAGG GCCCACCCGC CCTGCTGCCC TACTATGTGG CCAACGGCTC CTGGGTGGTG 
CCGCCAGAGC CCCGGCCCAG CTTCTTCTCC ATCTCCCCTC AAGACTGCCA GTTTGCTTTG 
GGGGGCCGCG GCGAGAAGCC AGAGCTGCAG ATGCTGACTG TATCAGAGGA GTTACCGTTT 
GACAACGTGG ATGGCGGCGT GTGGAAGCAA GGCTTCGACA TTTCCTACAG CCCGCACGAC 
TGGGACACCG AAGACCTGCA GGTGTTTGTG GTCCCCCACT CTCACAATGA CCCCGGCTGG 
CTCAAGACCT TTGACAAGTA CTACACAGAA CAGACCCAGC ACATCCTCAA CAACATGGTG 
TCCAAGCTGC AGGAGGACCC CCGGCGGCGC TTCATCTGGG CAGAAGTCTC CTTCTTTGCC 
AAGTGGTGGG ACAACATCAG TGCCCAAAAG AGAGCAGCAG TCCGAAGGCT GGTGGGAAAC 
GGGCAGCTGG AGATTGCAAC AGGAGGCTGG GTGATGCCGG ACGAGGCCAA CTCCCACTAT 
TTTGCATTGA TTGACCAGCT CATTGAGGGC CACCAGTGGC TGGAGAAGAA CCTGGGTGCA 
ACTCCGCGCT CGGGCTGGGC GGTGGACCCC TTTGGATACA GCCCCACCAT GCCTTACCTG 
CTGCGCCGTG CCAACCTGAC CAGCATGCTC ATTCAGAGGG TACACTACGC CATCAAGAAG 
CACTTTGCTG CCAGCCACAG CCTGGAGTTC ATGTGGAGGC AGACCTGGGA CTCGGATGCC 
AGCACAGACA TCTTCTGCCA CATGATGCCT TTCTACAGCT ACGATGTCCC CCATACGTGT 
GGCCCGGATC CCAAGATCTG CTGCCAGTTC GATTTCAAGC GCTTGCCTGG CGGGCGTATC 
AGCTGCCCTT GGAAGGTGCC GCCCCGGGCT ATCACGGAGG CCAATGTGGC TGAGAGGGCA 
GGCCTGCTCC TGGACCAGTA CAGGAAGAAG TCCCGGCTCT TCCGGAGCAA TGTGCTCCTG 
GTGCCGCTGG GCGATGACTT CCGCTACGAC AAGCCCCAGG AGTGGGACGC CCAGTTCTTC 
AACTACCAGC GGCTCTTCGA CTTCCTCAAC AGCAAGCCTG ACCTCCATGT GCAGGCCCAG 
TTTGGCACCC TCTCCGACTA TTTTGACGCT CTATACAAGA GGACAGGGGT GGAGCCGGGA 
GCCCGGCCTC CAGGTTTCCC TGTGCTGAGT GGGGATTTCT TCTCCTACGC GGACCGGGAG 
GACCACTACT GGACAGGCTA TTACACTTCA CGGCCTTTCT ACAAGAACCT GGACCGCGTC 
CTGGAAGCAC ACCTGCGGGG TGCAGAGATT CTGTACAGCC TGGCCGTGGC TCACGCCCGC 
CGCGCTGGAC TGGCCAGCCA GTTCCCACTC TCAAACTTCG CCCTTCTGAC GGACGCTCGG 
CGCACACTGG GGCTCTTCCA GCACCATGAC GCAATCACGG GCACCGCCAA GGAGGCCGTT 
GTGGTGGACT ATGGGGTCAG GCTTCTGCGC TCCCTTGTCA GCCTGAAACA GGTCATCATG 
AACGCAGCTC ACTACCTGGT GCTGGGGGAC AAGAAGGCCT ACCACTTTGA CCCAGAGGTG 
CCCTTCCTGC AGATGGATGA CACCCGCTTA AATCACGACG CCCTTCCAGA GCGCACAGTG 
ATCCAGCTGG AATCCTCACC CAGGTATGTG GTGCTGTTCA ACCCACTGGA GCAGGAGCGG 
CTCAGCGTGG TGTCCCTGCT GGTGAGCTCA CCCCGGGTGC GCGTGCTTTC GGAGGAGGGT 
CAGCCCCTGG CCGTGCAGGT CAGCGCGCAC TGGAGCTCTG CCACCGACAT GGTCCCTGAT 
GTCTATCAGG TGTCTGTGCC TGTCCGCCTG CCAGCGCTGG GTCTTGGCGT GCTGCAGCTG 
CAGCAGGGCC TGGATGGGCC CCGCACGCTG CCCTCCTCTG TGCGCGTCTA CCTACATGGG 
CGGCCGCTGT CCGTCAGCAG GAACGAGGCG TTCCCTCTCC GTGTCATTGA CTCTGGCACC 
AGTGACTTCG CCCTCAGCAA CCGCTACATG CAGGTCTGGT TCTCAGGCCT TACCGGGCTC 
CTCAAGAGCG TCCGAAGGGT GGATGAGGAG CAGGAACAGC GGGTGGACAT GGAGTTCCTC 
GTCTATGGCA CCCGCTCGTC CAAAGACAAG AGTGGAGCCT ACCTCTTCCT GCCTGACGGC 
GAGGCCAAGC CCTACGTCCC CAGGGACCCA CCCGTGCTGC GCGTCACAGA AGGCCCTTTC 
TTCTCAGAGG TGGTTGCCTG CTACGAGCAT GTTCACCAGG TGGTCCGGCT GTATAACCTG 
CCGGGGGTGG AGGGGCTGTC CCTGGATGTG TCGTCCTTGG TGGACATCCG GGACTACGTC 
AACAAGGAGC TGGCCCTGCG CATTCGCACT GACATCGATA GCCAGGGCAT CTTCTTCACA 
GACCTCAACG GCTTTCAGGT GCAGCCCCGA CGGTATCTGA AGAAGCTGCC CCTGCAGGCC 
AACTTCTACC CCATGCCCGT CATGGCCTAC CTCCAGGACG CTCAGAACCG CCTCACGCTG 
CACACAGCCC AGGCCCTGGG TGTCTCCAGC CTCCACAATG GCCAGCTAGA GGTGATCTTG 
GACCGGAGAC TGATGCAGGA CGACAACCGG GGCCTTGGCC AAGGGCTCAA GGACAACAAG 
AGAACCTGCA ACCACTTCCG CCTCCTGTTG GAACGGCGAA CCCTGGGCAG GGAGCCTGGC 
TTTTTCTCCA AACTGGCAGC CATGTTTAGG GGCTTGATCT TTCACAGCAG CAGGAACAGG 
AACCGAGAGG TCCGCGAGGG CTCCTCTGCA AGCTTCCCGT CCCTCCTCAG CCACCTGACC 
TCCATGTACC TGAACACCCC TGTGCTCGCC CTGCCTGTGG CCAGGAGGCA GGCCCCCGGC 
CCCGCTCTGC GCTCTTTTCA CCCTCTGGCC TCCTCACTGC CCTGTGACTT CCACCTGCTC 
AACCTGCGGA CGCTCCAGGC CGAGGAGGAT GGCTTGCCCT CGGCAGAAGC TGCGCTCCTC 
TTACACCGCA AGGGTTTTGA CTGTGGCCTT GAGGCCAAGA ACTTGGGCTT CAACTGCACC 
ACCAGCCAAG GCAAGGTGGC CCTGGGCAGC CTCTTCCATG GCCTGGACGT GGTTTTCCTG 
CAGCCAACCT CCTTGACCCT GCTGTACCCT CTGGCCTCGC CCTCCAATAG CACTGACGTC 
TATGTGGAGC CCATGGAGAT CGCCACCTTC CGCCTCCGCT TGGGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011953872.1
CDS	392..3919
Translation	MKLKKQVTVCGAAIFCVAVFSLYLMLDRVQHDPARHQNGGNFPRSQISVLQNRIEQLEQLLEENHEIISHIKDSVLELTANVEGPPALLPYYVANGSWVVPPEPRPSFFSISPQDCQFALGGRGEKPELQMLTVSEELPFDNVDGGVWKQGFDISYSPHDWDTEDLQVFVVPHSHNDPGWLKTFDKYYTEQTQHILNNMVSKLQEDPRRRFIWAEVSFFAKWWDNISAQKRAAVRRLVGNGQLEIATGGWVMPDEANSHYFALIDQLIEGHQWLEKNLGATPRSGWAVDPFGYSPTMPYLLRRANLTSMLIQRVHYAIKKHFAASHSLEFMWRQTWDSDASTDIFCHMMPFYSYDVPHTCGPDPKICCQFDFKRLPGGRISCPWKVPPRAITEANVAERAGLLLDQYRKKSRLFRSNVLLVPLGDDFRYDKPQEWDAQFFNYQRLFDFLNSKPDLHVQAQFGTLSDYFDALYKRTGVEPGARPPGFPVLSGDFFSYADREDHYWTGYYTSRPFYKNLDRVLEAHLRGAEILYSLAVAHARRAGLASQFPLSNFALLTDARRTLGLFQHHDAITGTAKEAVVVDYGVRLLRSLVSLKQVIMNAAHYLVLGDKKAYHFDPEVPFLQMDDTRLNHDALPERTVIQLESSPRYVVLFNPLEQERLSVVSLLVSSPRVRVLSEEGQPLAVQVSAHWSSATDMVPDVYQVSVPVRLPALGLGVLQLQQGLDGPRTLPSSVRVYLHGRPLSVSRNEAFPLRVIDSGTSDFALSNRYMQVWFSGLTGLLKSVRRVDEEQEQRVDMEFLVYGTRSSKDKSGAYLFLPDGEAKPYVPRDPPVLRVTEGPFFSEVVACYEHVHQVVRLYNLPGVEGLSLDVSSLVDIRDYVNKELALRIRTDIDSQGIFFTDLNGFQVQPRRYLKKLPLQANFYPMPVMAYLQDAQNRLTLHTAQALGVSSLHNGQLEVILDRRLMQDDNRGLGQGLKDNKRTCNHFRLLLERRTLGREPGFFSKLAAMFRGLIFHSSRNRNREVREGSSASFPSLLSHLTSMYLNTPVLALPVARRQAPGPALRSFHPLASSLPCDFHLLNLRTLQAEEDGLPSAEAALLLHRKGFDCGLEAKNLGFNCTTSQGKVALGSLFHGLDVVFLQPTSLTLLYPLASPSNSTDVYVEPMEIATFRLRLG

Target ORF information:

RefSeq Version	XM_012098482.2
Organism	Ovis aries(sheep)
Definition	Ovis aries mannosidase, alpha, class 2A, member 2 (MAN2A2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012098482.2

ORF Insert Sequence:

ATGAAGCTGA AAAAGCAGGT GACAGTGTGT GGGGCTGCTA TCTTCTGCGT GGCTGTCTTC 
TCGCTCTACC TCATGTTGGA CCGAGTACAG CATGATCCTG CTCGACACCA GAACGGTGGG 
AACTTCCCCC GGAGTCAGAT ATCTGTGCTA CAGAACCGCA TTGAACAGCT GGAACAGCTG 
TTGGAGGAGA ACCATGAGAT CATCAGCCAC ATCAAAGACT CTGTGCTGGA GCTGACAGCC 
AACGTGGAGG GCCCACCCGC CCTGCTGCCC TACTATGTGG CCAACGGCTC CTGGGTGGTG 
CCGCCAGAGC CCCGGCCCAG CTTCTTCTCC ATCTCCCCTC AAGACTGCCA GTTTGCTTTG 
GGGGGCCGCG GCGAGAAGCC AGAGCTGCAG ATGCTGACTG TATCAGAGGA GTTACCGTTT 
GACAACGTGG ATGGCGGCGT GTGGAAGCAA GGCTTCGACA TTTCCTACAG CCCGCACGAC 
TGGGACACCG AAGACCTGCA GGTGTTTGTG GTCCCCCACT CTCACAATGA CCCCGGCTGG 
CTCAAGACCT TTGACAAGTA CTACACAGAA CAGACCCAGC ACATCCTCAA CAACATGGTG 
TCCAAGCTGC AGGAGGACCC CCGGCGGCGC TTCATCTGGG CAGAAGTCTC CTTCTTTGCC 
AAGTGGTGGG ACAACATCAG TGCCCAAAAG AGAGCAGCAG TCCGAAGGCT GGTGGGAAAC 
GGGCAGCTGG AGATTGCAAC AGGAGGCTGG GTGATGCCGG ACGAGGCCAA CTCCCACTAT 
TTTGCATTGA TTGACCAGCT CATTGAGGGC CACCAGTGGC TGGAGAAGAA CCTGGGTGCA 
ACTCCGCGCT CGGGCTGGGC GGTGGACCCC TTTGGATACA GCCCCACCAT GCCTTACCTG 
CTGCGCCGTG CCAACCTGAC CAGCATGCTC ATTCAGAGGG TACACTACGC CATCAAGAAG 
CACTTTGCTG CCAGCCACAG CCTGGAGTTC ATGTGGAGGC AGACCTGGGA CTCGGATGCC 
AGCACAGACA TCTTCTGCCA CATGATGCCT TTCTACAGCT ACGATGTCCC CCATACGTGT 
GGCCCGGATC CCAAGATCTG CTGCCAGTTC GATTTCAAGC GCTTGCCTGG CGGGCGTATC 
AGCTGCCCTT GGAAGGTGCC GCCCCGGGCT ATCACGGAGG CCAATGTGGC TGAGAGGGCA 
GGCCTGCTCC TGGACCAGTA CAGGAAGAAG TCCCGGCTCT TCCGGAGCAA TGTGCTCCTG 
GTGCCGCTGG GCGATGACTT CCGCTACGAC AAGCCCCAGG AGTGGGACGC CCAGTTCTTC 
AACTACCAGC GGCTCTTCGA CTTCCTCAAC AGCAAGCCTG ACCTCCATGT GCAGGCCCAG 
TTTGGCACCC TCTCCGACTA TTTTGACGCT CTATACAAGA GGACAGGGGT GGAGCCGGGA 
GCCCGGCCTC CAGGTTTCCC TGTGCTGAGT GGGGATTTCT TCTCCTACGC GGACCGGGAG 
GACCACTACT GGACAGGCTA TTACACTTCA CGGCCTTTCT ACAAGAACCT GGACCGCGTC 
CTGGAAGCAC ACCTGCGGGG TGCAGAGATT CTGTACAGCC TGGCCGTGGC TCACGCCCGC 
CGCGCTGGAC TGGCCAGCCA GTTCCCACTC TCAAACTTCG CCCTTCTGAC GGACGCTCGG 
CGCACACTGG GGCTCTTCCA GCACCATGAC GCAATCACGG GCACCGCCAA GGAGGCCGTT 
GTGGTGGACT ATGGGGTCAG GCTTCTGCGC TCCCTTGTCA GCCTGAAACA GGTCATCATG 
AACGCAGCTC ACTACCTGGT GCTGGGGGAC AAGAAGGCCT ACCACTTTGA CCCAGAGGTG 
CCCTTCCTGC AGATGGATGA CACCCGCTTA AATCACGACG CCCTTCCAGA GCGCACAGTG 
ATCCAGCTGG AATCCTCACC CAGGTATGTG GTGCTGTTCA ACCCACTGGA GCAGGAGCGG 
CTCAGCGTGG TGTCCCTGCT GGTGAGCTCA CCCCGGGTGC GCGTGCTTTC GGAGGAGGGT 
CAGCCCCTGG CCGTGCAGGT CAGCGCGCAC TGGAGCTCTG CCACCGACAT GGTCCCTGAT 
GTCTATCAGG TGTCTGTGCC TGTCCGCCTG CCAGCGCTGG GTCTTGGCGT GCTGCAGCTG 
CAGCAGGGCC TGGATGGGCC CCGCACGCTG CCCTCCTCTG TGCGCGTCTA CCTACATGGG 
CGGCCGCTGT CCGTCAGCAG GAACGAGGCG TTCCCTCTCC GTGTCATTGA CTCTGGCACC 
AGTGACTTCG CCCTCAGCAA CCGCTACATG CAGGTCTGGT TCTCAGGCCT TACCGGGCTC 
CTCAAGAGCG TCCGAAGGGT GGATGAGGAG CAGGAACAGC GGGTGGACAT GGAGTTCCTC 
GTCTATGGCA CCCGCTCGTC CAAAGACAAG AGTGGAGCCT ACCTCTTCCT GCCTGACGGC 
GAGGCCAAGC CCTACGTCCC CAGGGACCCA CCCGTGCTGC GCGTCACAGA AGGCCCTTTC 
TTCTCAGAGG TGGTTGCCTG CTACGAGCAT GTTCACCAGG TGGTCCGGCT GTATAACCTG 
CCGGGGGTGG AGGGGCTGTC CCTGGATGTG TCGTCCTTGG TGGACATCCG GGACTACGTC 
AACAAGGAGC TGGCCCTGCG CATTCGCACT GACATCGATA GCCAGGGCAT CTTCTTCACA 
GACCTCAACG GCTTTCAGGT GCAGCCCCGA CGGTATCTGA AGAAGCTGCC CCTGCAGGCC 
AACTTCTACC CCATGCCCGT CATGGCCTAC CTCCAGGACG CTCAGAACCG CCTCACGCTG 
CACACAGCCC AGGCCCTGGG TGTCTCCAGC CTCCACAATG GCCAGCTAGA GGTGATCTTG 
GACCGGAGAC TGATGCAGGA CGACAACCGG GGCCTTGGCC AAGGGCTCAA GGACAACAAG 
AGAACCTGCA ACCACTTCCG CCTCCTGTTG GAACGGCGAA CCCTGGGCAG GGAGCCTGGC 
TTTTTCTCCA AACTGGCAGC CATGTTTAGG GGCTTGATCT TTCACAGCAG CAGGAACAGG 
AACCGAGAGG TCCGCGAGGG CTCCTCTGCA AGCTTCCCGT CCCTCCTCAG CCACCTGACC 
TCCATGTACC TGAACACCCC TGTGCTCGCC CTGCCTGTGG CCAGGAGGCA GGCCCCCGGC 
CCCGCTCTGC GCTCTTTTCA CCCTCTGGCC TCCTCACTGC CCTGTGACTT CCACCTGCTC 
AACCTGCGGA CGCTCCAGGC CGAGGAGGAT GGCTTGCCCT CGGCAGAAGC TGCGCTCCTC 
TTACACCGCA AGGGTTTTGA CTGTGGCCTT GAGGCCAAGA ACTTGGGCTT CAACTGCACC 
ACCAGCCAAG GCAAGGTGGC CCTGGGCAGC CTCTTCCATG GCCTGGACGT GGTTTTCCTG 
CAGCCAACCT CCTTGACCCT GCTGTACCCT CTGGCCTCGC CCTCCAATAG CACTGACGTC 
TATGTGGAGC CCATGGAGAT CGCCACCTTC CGCCTCCGCT TGGGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAN2A2 ( XM_015101896.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_014957382.1 Ovis aries mannosidase, alpha, class 2A, member 2 (MAN2A2), transcript variant X2, mRNA.

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CloneID	OSm27643
Clone ID Related Accession (Same CDS sequence)	XM_012098482.2 , XM_015101896.1 , XM_012098481.2 , XM_012098483.1
Accession Version	XM_015101896.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3528bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries(sheep)
Product	alpha-mannosidase 2x isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014639027.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCTGA AAAAGCAGGT GACAGTGTGT GGGGCTGCTA TCTTCTGCGT GGCTGTCTTC 
TCGCTCTACC TCATGTTGGA CCGAGTACAG CATGATCCTG CTCGACACCA GAACGGTGGG 
AACTTCCCCC GGAGTCAGAT ATCTGTGCTA CAGAACCGCA TTGAACAGCT GGAACAGCTG 
TTGGAGGAGA ACCATGAGAT CATCAGCCAC ATCAAAGACT CTGTGCTGGA GCTGACAGCC 
AACGTGGAGG GCCCACCCGC CCTGCTGCCC TACTATGTGG CCAACGGCTC CTGGGTGGTG 
CCGCCAGAGC CCCGGCCCAG CTTCTTCTCC ATCTCCCCTC AAGACTGCCA GTTTGCTTTG 
GGGGGCCGCG GCGAGAAGCC AGAGCTGCAG ATGCTGACTG TATCAGAGGA GTTACCGTTT 
GACAACGTGG ATGGCGGCGT GTGGAAGCAA GGCTTCGACA TTTCCTACAG CCCGCACGAC 
TGGGACACCG AAGACCTGCA GGTGTTTGTG GTCCCCCACT CTCACAATGA CCCCGGCTGG 
CTCAAGACCT TTGACAAGTA CTACACAGAA CAGACCCAGC ACATCCTCAA CAACATGGTG 
TCCAAGCTGC AGGAGGACCC CCGGCGGCGC TTCATCTGGG CAGAAGTCTC CTTCTTTGCC 
AAGTGGTGGG ACAACATCAG TGCCCAAAAG AGAGCAGCAG TCCGAAGGCT GGTGGGAAAC 
GGGCAGCTGG AGATTGCAAC AGGAGGCTGG GTGATGCCGG ACGAGGCCAA CTCCCACTAT 
TTTGCATTGA TTGACCAGCT CATTGAGGGC CACCAGTGGC TGGAGAAGAA CCTGGGTGCA 
ACTCCGCGCT CGGGCTGGGC GGTGGACCCC TTTGGATACA GCCCCACCAT GCCTTACCTG 
CTGCGCCGTG CCAACCTGAC CAGCATGCTC ATTCAGAGGG TACACTACGC CATCAAGAAG 
CACTTTGCTG CCAGCCACAG CCTGGAGTTC ATGTGGAGGC AGACCTGGGA CTCGGATGCC 
AGCACAGACA TCTTCTGCCA CATGATGCCT TTCTACAGCT ACGATGTCCC CCATACGTGT 
GGCCCGGATC CCAAGATCTG CTGCCAGTTC GATTTCAAGC GCTTGCCTGG CGGGCGTATC 
AGCTGCCCTT GGAAGGTGCC GCCCCGGGCT ATCACGGAGG CCAATGTGGC TGAGAGGGCA 
GGCCTGCTCC TGGACCAGTA CAGGAAGAAG TCCCGGCTCT TCCGGAGCAA TGTGCTCCTG 
GTGCCGCTGG GCGATGACTT CCGCTACGAC AAGCCCCAGG AGTGGGACGC CCAGTTCTTC 
AACTACCAGC GGCTCTTCGA CTTCCTCAAC AGCAAGCCTG ACCTCCATGT GCAGGCCCAG 
TTTGGCACCC TCTCCGACTA TTTTGACGCT CTATACAAGA GGACAGGGGT GGAGCCGGGA 
GCCCGGCCTC CAGGTTTCCC TGTGCTGAGT GGGGATTTCT TCTCCTACGC GGACCGGGAG 
GACCACTACT GGACAGGCTA TTACACTTCA CGGCCTTTCT ACAAGAACCT GGACCGCGTC 
CTGGAAGCAC ACCTGCGGGG TGCAGAGATT CTGTACAGCC TGGCCGTGGC TCACGCCCGC 
CGCGCTGGAC TGGCCAGCCA GTTCCCACTC TCAAACTTCG CCCTTCTGAC GGACGCTCGG 
CGCACACTGG GGCTCTTCCA GCACCATGAC GCAATCACGG GCACCGCCAA GGAGGCCGTT 
GTGGTGGACT ATGGGGTCAG GCTTCTGCGC TCCCTTGTCA GCCTGAAACA GGTCATCATG 
AACGCAGCTC ACTACCTGGT GCTGGGGGAC AAGAAGGCCT ACCACTTTGA CCCAGAGGTG 
CCCTTCCTGC AGATGGATGA CACCCGCTTA AATCACGACG CCCTTCCAGA GCGCACAGTG 
ATCCAGCTGG AATCCTCACC CAGGTATGTG GTGCTGTTCA ACCCACTGGA GCAGGAGCGG 
CTCAGCGTGG TGTCCCTGCT GGTGAGCTCA CCCCGGGTGC GCGTGCTTTC GGAGGAGGGT 
CAGCCCCTGG CCGTGCAGGT CAGCGCGCAC TGGAGCTCTG CCACCGACAT GGTCCCTGAT 
GTCTATCAGG TGTCTGTGCC TGTCCGCCTG CCAGCGCTGG GTCTTGGCGT GCTGCAGCTG 
CAGCAGGGCC TGGATGGGCC CCGCACGCTG CCCTCCTCTG TGCGCGTCTA CCTACATGGG 
CGGCCGCTGT CCGTCAGCAG GAACGAGGCG TTCCCTCTCC GTGTCATTGA CTCTGGCACC 
AGTGACTTCG CCCTCAGCAA CCGCTACATG CAGGTCTGGT TCTCAGGCCT TACCGGGCTC 
CTCAAGAGCG TCCGAAGGGT GGATGAGGAG CAGGAACAGC GGGTGGACAT GGAGTTCCTC 
GTCTATGGCA CCCGCTCGTC CAAAGACAAG AGTGGAGCCT ACCTCTTCCT GCCTGACGGC 
GAGGCCAAGC CCTACGTCCC CAGGGACCCA CCCGTGCTGC GCGTCACAGA AGGCCCTTTC 
TTCTCAGAGG TGGTTGCCTG CTACGAGCAT GTTCACCAGG TGGTCCGGCT GTATAACCTG 
CCGGGGGTGG AGGGGCTGTC CCTGGATGTG TCGTCCTTGG TGGACATCCG GGACTACGTC 
AACAAGGAGC TGGCCCTGCG CATTCGCACT GACATCGATA GCCAGGGCAT CTTCTTCACA 
GACCTCAACG GCTTTCAGGT GCAGCCCCGA CGGTATCTGA AGAAGCTGCC CCTGCAGGCC 
AACTTCTACC CCATGCCCGT CATGGCCTAC CTCCAGGACG CTCAGAACCG CCTCACGCTG 
CACACAGCCC AGGCCCTGGG TGTCTCCAGC CTCCACAATG GCCAGCTAGA GGTGATCTTG 
GACCGGAGAC TGATGCAGGA CGACAACCGG GGCCTTGGCC AAGGGCTCAA GGACAACAAG 
AGAACCTGCA ACCACTTCCG CCTCCTGTTG GAACGGCGAA CCCTGGGCAG GGAGCCTGGC 
TTTTTCTCCA AACTGGCAGC CATGTTTAGG GGCTTGATCT TTCACAGCAG CAGGAACAGG 
AACCGAGAGG TCCGCGAGGG CTCCTCTGCA AGCTTCCCGT CCCTCCTCAG CCACCTGACC 
TCCATGTACC TGAACACCCC TGTGCTCGCC CTGCCTGTGG CCAGGAGGCA GGCCCCCGGC 
CCCGCTCTGC GCTCTTTTCA CCCTCTGGCC TCCTCACTGC CCTGTGACTT CCACCTGCTC 
AACCTGCGGA CGCTCCAGGC CGAGGAGGAT GGCTTGCCCT CGGCAGAAGC TGCGCTCCTC 
TTACACCGCA AGGGTTTTGA CTGTGGCCTT GAGGCCAAGA ACTTGGGCTT CAACTGCACC 
ACCAGCCAAG GCAAGGTGGC CCTGGGCAGC CTCTTCCATG GCCTGGACGT GGTTTTCCTG 
CAGCCAACCT CCTTGACCCT GCTGTACCCT CTGGCCTCGC CCTCCAATAG CACTGACGTC 
TATGTGGAGC CCATGGAGAT CGCCACCTTC CGCCTCCGCT TGGGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014957382.1
CDS	327..3854
Translation	MKLKKQVTVCGAAIFCVAVFSLYLMLDRVQHDPARHQNGGNFPRSQISVLQNRIEQLEQLLEENHEIISHIKDSVLELTANVEGPPALLPYYVANGSWVVPPEPRPSFFSISPQDCQFALGGRGEKPELQMLTVSEELPFDNVDGGVWKQGFDISYSPHDWDTEDLQVFVVPHSHNDPGWLKTFDKYYTEQTQHILNNMVSKLQEDPRRRFIWAEVSFFAKWWDNISAQKRAAVRRLVGNGQLEIATGGWVMPDEANSHYFALIDQLIEGHQWLEKNLGATPRSGWAVDPFGYSPTMPYLLRRANLTSMLIQRVHYAIKKHFAASHSLEFMWRQTWDSDASTDIFCHMMPFYSYDVPHTCGPDPKICCQFDFKRLPGGRISCPWKVPPRAITEANVAERAGLLLDQYRKKSRLFRSNVLLVPLGDDFRYDKPQEWDAQFFNYQRLFDFLNSKPDLHVQAQFGTLSDYFDALYKRTGVEPGARPPGFPVLSGDFFSYADREDHYWTGYYTSRPFYKNLDRVLEAHLRGAEILYSLAVAHARRAGLASQFPLSNFALLTDARRTLGLFQHHDAITGTAKEAVVVDYGVRLLRSLVSLKQVIMNAAHYLVLGDKKAYHFDPEVPFLQMDDTRLNHDALPERTVIQLESSPRYVVLFNPLEQERLSVVSLLVSSPRVRVLSEEGQPLAVQVSAHWSSATDMVPDVYQVSVPVRLPALGLGVLQLQQGLDGPRTLPSSVRVYLHGRPLSVSRNEAFPLRVIDSGTSDFALSNRYMQVWFSGLTGLLKSVRRVDEEQEQRVDMEFLVYGTRSSKDKSGAYLFLPDGEAKPYVPRDPPVLRVTEGPFFSEVVACYEHVHQVVRLYNLPGVEGLSLDVSSLVDIRDYVNKELALRIRTDIDSQGIFFTDLNGFQVQPRRYLKKLPLQANFYPMPVMAYLQDAQNRLTLHTAQALGVSSLHNGQLEVILDRRLMQDDNRGLGQGLKDNKRTCNHFRLLLERRTLGREPGFFSKLAAMFRGLIFHSSRNRNREVREGSSASFPSLLSHLTSMYLNTPVLALPVARRQAPGPALRSFHPLASSLPCDFHLLNLRTLQAEEDGLPSAEAALLLHRKGFDCGLEAKNLGFNCTTSQGKVALGSLFHGLDVVFLQPTSLTLLYPLASPSNSTDVYVEPMEIATFRLRLG

Target ORF information:

RefSeq Version	XM_015101896.1
Organism	Ovis aries(sheep)
Definition	Ovis aries mannosidase, alpha, class 2A, member 2 (MAN2A2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015101896.1

ORF Insert Sequence:

ATGAAGCTGA AAAAGCAGGT GACAGTGTGT GGGGCTGCTA TCTTCTGCGT GGCTGTCTTC 
TCGCTCTACC TCATGTTGGA CCGAGTACAG CATGATCCTG CTCGACACCA GAACGGTGGG 
AACTTCCCCC GGAGTCAGAT ATCTGTGCTA CAGAACCGCA TTGAACAGCT GGAACAGCTG 
TTGGAGGAGA ACCATGAGAT CATCAGCCAC ATCAAAGACT CTGTGCTGGA GCTGACAGCC 
AACGTGGAGG GCCCACCCGC CCTGCTGCCC TACTATGTGG CCAACGGCTC CTGGGTGGTG 
CCGCCAGAGC CCCGGCCCAG CTTCTTCTCC ATCTCCCCTC AAGACTGCCA GTTTGCTTTG 
GGGGGCCGCG GCGAGAAGCC AGAGCTGCAG ATGCTGACTG TATCAGAGGA GTTACCGTTT 
GACAACGTGG ATGGCGGCGT GTGGAAGCAA GGCTTCGACA TTTCCTACAG CCCGCACGAC 
TGGGACACCG AAGACCTGCA GGTGTTTGTG GTCCCCCACT CTCACAATGA CCCCGGCTGG 
CTCAAGACCT TTGACAAGTA CTACACAGAA CAGACCCAGC ACATCCTCAA CAACATGGTG 
TCCAAGCTGC AGGAGGACCC CCGGCGGCGC TTCATCTGGG CAGAAGTCTC CTTCTTTGCC 
AAGTGGTGGG ACAACATCAG TGCCCAAAAG AGAGCAGCAG TCCGAAGGCT GGTGGGAAAC 
GGGCAGCTGG AGATTGCAAC AGGAGGCTGG GTGATGCCGG ACGAGGCCAA CTCCCACTAT 
TTTGCATTGA TTGACCAGCT CATTGAGGGC CACCAGTGGC TGGAGAAGAA CCTGGGTGCA 
ACTCCGCGCT CGGGCTGGGC GGTGGACCCC TTTGGATACA GCCCCACCAT GCCTTACCTG 
CTGCGCCGTG CCAACCTGAC CAGCATGCTC ATTCAGAGGG TACACTACGC CATCAAGAAG 
CACTTTGCTG CCAGCCACAG CCTGGAGTTC ATGTGGAGGC AGACCTGGGA CTCGGATGCC 
AGCACAGACA TCTTCTGCCA CATGATGCCT TTCTACAGCT ACGATGTCCC CCATACGTGT 
GGCCCGGATC CCAAGATCTG CTGCCAGTTC GATTTCAAGC GCTTGCCTGG CGGGCGTATC 
AGCTGCCCTT GGAAGGTGCC GCCCCGGGCT ATCACGGAGG CCAATGTGGC TGAGAGGGCA 
GGCCTGCTCC TGGACCAGTA CAGGAAGAAG TCCCGGCTCT TCCGGAGCAA TGTGCTCCTG 
GTGCCGCTGG GCGATGACTT CCGCTACGAC AAGCCCCAGG AGTGGGACGC CCAGTTCTTC 
AACTACCAGC GGCTCTTCGA CTTCCTCAAC AGCAAGCCTG ACCTCCATGT GCAGGCCCAG 
TTTGGCACCC TCTCCGACTA TTTTGACGCT CTATACAAGA GGACAGGGGT GGAGCCGGGA 
GCCCGGCCTC CAGGTTTCCC TGTGCTGAGT GGGGATTTCT TCTCCTACGC GGACCGGGAG 
GACCACTACT GGACAGGCTA TTACACTTCA CGGCCTTTCT ACAAGAACCT GGACCGCGTC 
CTGGAAGCAC ACCTGCGGGG TGCAGAGATT CTGTACAGCC TGGCCGTGGC TCACGCCCGC 
CGCGCTGGAC TGGCCAGCCA GTTCCCACTC TCAAACTTCG CCCTTCTGAC GGACGCTCGG 
CGCACACTGG GGCTCTTCCA GCACCATGAC GCAATCACGG GCACCGCCAA GGAGGCCGTT 
GTGGTGGACT ATGGGGTCAG GCTTCTGCGC TCCCTTGTCA GCCTGAAACA GGTCATCATG 
AACGCAGCTC ACTACCTGGT GCTGGGGGAC AAGAAGGCCT ACCACTTTGA CCCAGAGGTG 
CCCTTCCTGC AGATGGATGA CACCCGCTTA AATCACGACG CCCTTCCAGA GCGCACAGTG 
ATCCAGCTGG AATCCTCACC CAGGTATGTG GTGCTGTTCA ACCCACTGGA GCAGGAGCGG 
CTCAGCGTGG TGTCCCTGCT GGTGAGCTCA CCCCGGGTGC GCGTGCTTTC GGAGGAGGGT 
CAGCCCCTGG CCGTGCAGGT CAGCGCGCAC TGGAGCTCTG CCACCGACAT GGTCCCTGAT 
GTCTATCAGG TGTCTGTGCC TGTCCGCCTG CCAGCGCTGG GTCTTGGCGT GCTGCAGCTG 
CAGCAGGGCC TGGATGGGCC CCGCACGCTG CCCTCCTCTG TGCGCGTCTA CCTACATGGG 
CGGCCGCTGT CCGTCAGCAG GAACGAGGCG TTCCCTCTCC GTGTCATTGA CTCTGGCACC 
AGTGACTTCG CCCTCAGCAA CCGCTACATG CAGGTCTGGT TCTCAGGCCT TACCGGGCTC 
CTCAAGAGCG TCCGAAGGGT GGATGAGGAG CAGGAACAGC GGGTGGACAT GGAGTTCCTC 
GTCTATGGCA CCCGCTCGTC CAAAGACAAG AGTGGAGCCT ACCTCTTCCT GCCTGACGGC 
GAGGCCAAGC CCTACGTCCC CAGGGACCCA CCCGTGCTGC GCGTCACAGA AGGCCCTTTC 
TTCTCAGAGG TGGTTGCCTG CTACGAGCAT GTTCACCAGG TGGTCCGGCT GTATAACCTG 
CCGGGGGTGG AGGGGCTGTC CCTGGATGTG TCGTCCTTGG TGGACATCCG GGACTACGTC 
AACAAGGAGC TGGCCCTGCG CATTCGCACT GACATCGATA GCCAGGGCAT CTTCTTCACA 
GACCTCAACG GCTTTCAGGT GCAGCCCCGA CGGTATCTGA AGAAGCTGCC CCTGCAGGCC 
AACTTCTACC CCATGCCCGT CATGGCCTAC CTCCAGGACG CTCAGAACCG CCTCACGCTG 
CACACAGCCC AGGCCCTGGG TGTCTCCAGC CTCCACAATG GCCAGCTAGA GGTGATCTTG 
GACCGGAGAC TGATGCAGGA CGACAACCGG GGCCTTGGCC AAGGGCTCAA GGACAACAAG 
AGAACCTGCA ACCACTTCCG CCTCCTGTTG GAACGGCGAA CCCTGGGCAG GGAGCCTGGC 
TTTTTCTCCA AACTGGCAGC CATGTTTAGG GGCTTGATCT TTCACAGCAG CAGGAACAGG 
AACCGAGAGG TCCGCGAGGG CTCCTCTGCA AGCTTCCCGT CCCTCCTCAG CCACCTGACC 
TCCATGTACC TGAACACCCC TGTGCTCGCC CTGCCTGTGG CCAGGAGGCA GGCCCCCGGC 
CCCGCTCTGC GCTCTTTTCA CCCTCTGGCC TCCTCACTGC CCTGTGACTT CCACCTGCTC 
AACCTGCGGA CGCTCCAGGC CGAGGAGGAT GGCTTGCCCT CGGCAGAAGC TGCGCTCCTC 
TTACACCGCA AGGGTTTTGA CTGTGGCCTT GAGGCCAAGA ACTTGGGCTT CAACTGCACC 
ACCAGCCAAG GCAAGGTGGC CCTGGGCAGC CTCTTCCATG GCCTGGACGT GGTTTTCCTG 
CAGCCAACCT CCTTGACCCT GCTGTACCCT CTGGCCTCGC CCTCCAATAG CACTGACGTC 
TATGTGGAGC CCATGGAGAT CGCCACCTTC CGCCTCCGCT TGGGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAN2A2 ( XM_012098481.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_011953871.1 Ovis aries mannosidase, alpha, class 2A, member 2 (MAN2A2), transcript variant X7, mRNA.

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CloneID	OSm27643
Clone ID Related Accession (Same CDS sequence)	XM_012098482.2 , XM_015101896.1 , XM_012098481.2 , XM_012098483.1
Accession Version	XM_012098481.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3528bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries(sheep)
Product	alpha-mannosidase 2x isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014639027.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012098481.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCTGA AAAAGCAGGT GACAGTGTGT GGGGCTGCTA TCTTCTGCGT GGCTGTCTTC 
TCGCTCTACC TCATGTTGGA CCGAGTACAG CATGATCCTG CTCGACACCA GAACGGTGGG 
AACTTCCCCC GGAGTCAGAT ATCTGTGCTA CAGAACCGCA TTGAACAGCT GGAACAGCTG 
TTGGAGGAGA ACCATGAGAT CATCAGCCAC ATCAAAGACT CTGTGCTGGA GCTGACAGCC 
AACGTGGAGG GCCCACCCGC CCTGCTGCCC TACTATGTGG CCAACGGCTC CTGGGTGGTG 
CCGCCAGAGC CCCGGCCCAG CTTCTTCTCC ATCTCCCCTC AAGACTGCCA GTTTGCTTTG 
GGGGGCCGCG GCGAGAAGCC AGAGCTGCAG ATGCTGACTG TATCAGAGGA GTTACCGTTT 
GACAACGTGG ATGGCGGCGT GTGGAAGCAA GGCTTCGACA TTTCCTACAG CCCGCACGAC 
TGGGACACCG AAGACCTGCA GGTGTTTGTG GTCCCCCACT CTCACAATGA CCCCGGCTGG 
CTCAAGACCT TTGACAAGTA CTACACAGAA CAGACCCAGC ACATCCTCAA CAACATGGTG 
TCCAAGCTGC AGGAGGACCC CCGGCGGCGC TTCATCTGGG CAGAAGTCTC CTTCTTTGCC 
AAGTGGTGGG ACAACATCAG TGCCCAAAAG AGAGCAGCAG TCCGAAGGCT GGTGGGAAAC 
GGGCAGCTGG AGATTGCAAC AGGAGGCTGG GTGATGCCGG ACGAGGCCAA CTCCCACTAT 
TTTGCATTGA TTGACCAGCT CATTGAGGGC CACCAGTGGC TGGAGAAGAA CCTGGGTGCA 
ACTCCGCGCT CGGGCTGGGC GGTGGACCCC TTTGGATACA GCCCCACCAT GCCTTACCTG 
CTGCGCCGTG CCAACCTGAC CAGCATGCTC ATTCAGAGGG TACACTACGC CATCAAGAAG 
CACTTTGCTG CCAGCCACAG CCTGGAGTTC ATGTGGAGGC AGACCTGGGA CTCGGATGCC 
AGCACAGACA TCTTCTGCCA CATGATGCCT TTCTACAGCT ACGATGTCCC CCATACGTGT 
GGCCCGGATC CCAAGATCTG CTGCCAGTTC GATTTCAAGC GCTTGCCTGG CGGGCGTATC 
AGCTGCCCTT GGAAGGTGCC GCCCCGGGCT ATCACGGAGG CCAATGTGGC TGAGAGGGCA 
GGCCTGCTCC TGGACCAGTA CAGGAAGAAG TCCCGGCTCT TCCGGAGCAA TGTGCTCCTG 
GTGCCGCTGG GCGATGACTT CCGCTACGAC AAGCCCCAGG AGTGGGACGC CCAGTTCTTC 
AACTACCAGC GGCTCTTCGA CTTCCTCAAC AGCAAGCCTG ACCTCCATGT GCAGGCCCAG 
TTTGGCACCC TCTCCGACTA TTTTGACGCT CTATACAAGA GGACAGGGGT GGAGCCGGGA 
GCCCGGCCTC CAGGTTTCCC TGTGCTGAGT GGGGATTTCT TCTCCTACGC GGACCGGGAG 
GACCACTACT GGACAGGCTA TTACACTTCA CGGCCTTTCT ACAAGAACCT GGACCGCGTC 
CTGGAAGCAC ACCTGCGGGG TGCAGAGATT CTGTACAGCC TGGCCGTGGC TCACGCCCGC 
CGCGCTGGAC TGGCCAGCCA GTTCCCACTC TCAAACTTCG CCCTTCTGAC GGACGCTCGG 
CGCACACTGG GGCTCTTCCA GCACCATGAC GCAATCACGG GCACCGCCAA GGAGGCCGTT 
GTGGTGGACT ATGGGGTCAG GCTTCTGCGC TCCCTTGTCA GCCTGAAACA GGTCATCATG 
AACGCAGCTC ACTACCTGGT GCTGGGGGAC AAGAAGGCCT ACCACTTTGA CCCAGAGGTG 
CCCTTCCTGC AGATGGATGA CACCCGCTTA AATCACGACG CCCTTCCAGA GCGCACAGTG 
ATCCAGCTGG AATCCTCACC CAGGTATGTG GTGCTGTTCA ACCCACTGGA GCAGGAGCGG 
CTCAGCGTGG TGTCCCTGCT GGTGAGCTCA CCCCGGGTGC GCGTGCTTTC GGAGGAGGGT 
CAGCCCCTGG CCGTGCAGGT CAGCGCGCAC TGGAGCTCTG CCACCGACAT GGTCCCTGAT 
GTCTATCAGG TGTCTGTGCC TGTCCGCCTG CCAGCGCTGG GTCTTGGCGT GCTGCAGCTG 
CAGCAGGGCC TGGATGGGCC CCGCACGCTG CCCTCCTCTG TGCGCGTCTA CCTACATGGG 
CGGCCGCTGT CCGTCAGCAG GAACGAGGCG TTCCCTCTCC GTGTCATTGA CTCTGGCACC 
AGTGACTTCG CCCTCAGCAA CCGCTACATG CAGGTCTGGT TCTCAGGCCT TACCGGGCTC 
CTCAAGAGCG TCCGAAGGGT GGATGAGGAG CAGGAACAGC GGGTGGACAT GGAGTTCCTC 
GTCTATGGCA CCCGCTCGTC CAAAGACAAG AGTGGAGCCT ACCTCTTCCT GCCTGACGGC 
GAGGCCAAGC CCTACGTCCC CAGGGACCCA CCCGTGCTGC GCGTCACAGA AGGCCCTTTC 
TTCTCAGAGG TGGTTGCCTG CTACGAGCAT GTTCACCAGG TGGTCCGGCT GTATAACCTG 
CCGGGGGTGG AGGGGCTGTC CCTGGATGTG TCGTCCTTGG TGGACATCCG GGACTACGTC 
AACAAGGAGC TGGCCCTGCG CATTCGCACT GACATCGATA GCCAGGGCAT CTTCTTCACA 
GACCTCAACG GCTTTCAGGT GCAGCCCCGA CGGTATCTGA AGAAGCTGCC CCTGCAGGCC 
AACTTCTACC CCATGCCCGT CATGGCCTAC CTCCAGGACG CTCAGAACCG CCTCACGCTG 
CACACAGCCC AGGCCCTGGG TGTCTCCAGC CTCCACAATG GCCAGCTAGA GGTGATCTTG 
GACCGGAGAC TGATGCAGGA CGACAACCGG GGCCTTGGCC AAGGGCTCAA GGACAACAAG 
AGAACCTGCA ACCACTTCCG CCTCCTGTTG GAACGGCGAA CCCTGGGCAG GGAGCCTGGC 
TTTTTCTCCA AACTGGCAGC CATGTTTAGG GGCTTGATCT TTCACAGCAG CAGGAACAGG 
AACCGAGAGG TCCGCGAGGG CTCCTCTGCA AGCTTCCCGT CCCTCCTCAG CCACCTGACC 
TCCATGTACC TGAACACCCC TGTGCTCGCC CTGCCTGTGG CCAGGAGGCA GGCCCCCGGC 
CCCGCTCTGC GCTCTTTTCA CCCTCTGGCC TCCTCACTGC CCTGTGACTT CCACCTGCTC 
AACCTGCGGA CGCTCCAGGC CGAGGAGGAT GGCTTGCCCT CGGCAGAAGC TGCGCTCCTC 
TTACACCGCA AGGGTTTTGA CTGTGGCCTT GAGGCCAAGA ACTTGGGCTT CAACTGCACC 
ACCAGCCAAG GCAAGGTGGC CCTGGGCAGC CTCTTCCATG GCCTGGACGT GGTTTTCCTG 
CAGCCAACCT CCTTGACCCT GCTGTACCCT CTGGCCTCGC CCTCCAATAG CACTGACGTC 
TATGTGGAGC CCATGGAGAT CGCCACCTTC CGCCTCCGCT TGGGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011953871.1
CDS	396..3923
Translation	MKLKKQVTVCGAAIFCVAVFSLYLMLDRVQHDPARHQNGGNFPRSQISVLQNRIEQLEQLLEENHEIISHIKDSVLELTANVEGPPALLPYYVANGSWVVPPEPRPSFFSISPQDCQFALGGRGEKPELQMLTVSEELPFDNVDGGVWKQGFDISYSPHDWDTEDLQVFVVPHSHNDPGWLKTFDKYYTEQTQHILNNMVSKLQEDPRRRFIWAEVSFFAKWWDNISAQKRAAVRRLVGNGQLEIATGGWVMPDEANSHYFALIDQLIEGHQWLEKNLGATPRSGWAVDPFGYSPTMPYLLRRANLTSMLIQRVHYAIKKHFAASHSLEFMWRQTWDSDASTDIFCHMMPFYSYDVPHTCGPDPKICCQFDFKRLPGGRISCPWKVPPRAITEANVAERAGLLLDQYRKKSRLFRSNVLLVPLGDDFRYDKPQEWDAQFFNYQRLFDFLNSKPDLHVQAQFGTLSDYFDALYKRTGVEPGARPPGFPVLSGDFFSYADREDHYWTGYYTSRPFYKNLDRVLEAHLRGAEILYSLAVAHARRAGLASQFPLSNFALLTDARRTLGLFQHHDAITGTAKEAVVVDYGVRLLRSLVSLKQVIMNAAHYLVLGDKKAYHFDPEVPFLQMDDTRLNHDALPERTVIQLESSPRYVVLFNPLEQERLSVVSLLVSSPRVRVLSEEGQPLAVQVSAHWSSATDMVPDVYQVSVPVRLPALGLGVLQLQQGLDGPRTLPSSVRVYLHGRPLSVSRNEAFPLRVIDSGTSDFALSNRYMQVWFSGLTGLLKSVRRVDEEQEQRVDMEFLVYGTRSSKDKSGAYLFLPDGEAKPYVPRDPPVLRVTEGPFFSEVVACYEHVHQVVRLYNLPGVEGLSLDVSSLVDIRDYVNKELALRIRTDIDSQGIFFTDLNGFQVQPRRYLKKLPLQANFYPMPVMAYLQDAQNRLTLHTAQALGVSSLHNGQLEVILDRRLMQDDNRGLGQGLKDNKRTCNHFRLLLERRTLGREPGFFSKLAAMFRGLIFHSSRNRNREVREGSSASFPSLLSHLTSMYLNTPVLALPVARRQAPGPALRSFHPLASSLPCDFHLLNLRTLQAEEDGLPSAEAALLLHRKGFDCGLEAKNLGFNCTTSQGKVALGSLFHGLDVVFLQPTSLTLLYPLASPSNSTDVYVEPMEIATFRLRLG

Target ORF information:

RefSeq Version	XM_012098481.2
Organism	Ovis aries(sheep)
Definition	Ovis aries mannosidase, alpha, class 2A, member 2 (MAN2A2), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012098481.2

ORF Insert Sequence:

ATGAAGCTGA AAAAGCAGGT GACAGTGTGT GGGGCTGCTA TCTTCTGCGT GGCTGTCTTC 
TCGCTCTACC TCATGTTGGA CCGAGTACAG CATGATCCTG CTCGACACCA GAACGGTGGG 
AACTTCCCCC GGAGTCAGAT ATCTGTGCTA CAGAACCGCA TTGAACAGCT GGAACAGCTG 
TTGGAGGAGA ACCATGAGAT CATCAGCCAC ATCAAAGACT CTGTGCTGGA GCTGACAGCC 
AACGTGGAGG GCCCACCCGC CCTGCTGCCC TACTATGTGG CCAACGGCTC CTGGGTGGTG 
CCGCCAGAGC CCCGGCCCAG CTTCTTCTCC ATCTCCCCTC AAGACTGCCA GTTTGCTTTG 
GGGGGCCGCG GCGAGAAGCC AGAGCTGCAG ATGCTGACTG TATCAGAGGA GTTACCGTTT 
GACAACGTGG ATGGCGGCGT GTGGAAGCAA GGCTTCGACA TTTCCTACAG CCCGCACGAC 
TGGGACACCG AAGACCTGCA GGTGTTTGTG GTCCCCCACT CTCACAATGA CCCCGGCTGG 
CTCAAGACCT TTGACAAGTA CTACACAGAA CAGACCCAGC ACATCCTCAA CAACATGGTG 
TCCAAGCTGC AGGAGGACCC CCGGCGGCGC TTCATCTGGG CAGAAGTCTC CTTCTTTGCC 
AAGTGGTGGG ACAACATCAG TGCCCAAAAG AGAGCAGCAG TCCGAAGGCT GGTGGGAAAC 
GGGCAGCTGG AGATTGCAAC AGGAGGCTGG GTGATGCCGG ACGAGGCCAA CTCCCACTAT 
TTTGCATTGA TTGACCAGCT CATTGAGGGC CACCAGTGGC TGGAGAAGAA CCTGGGTGCA 
ACTCCGCGCT CGGGCTGGGC GGTGGACCCC TTTGGATACA GCCCCACCAT GCCTTACCTG 
CTGCGCCGTG CCAACCTGAC CAGCATGCTC ATTCAGAGGG TACACTACGC CATCAAGAAG 
CACTTTGCTG CCAGCCACAG CCTGGAGTTC ATGTGGAGGC AGACCTGGGA CTCGGATGCC 
AGCACAGACA TCTTCTGCCA CATGATGCCT TTCTACAGCT ACGATGTCCC CCATACGTGT 
GGCCCGGATC CCAAGATCTG CTGCCAGTTC GATTTCAAGC GCTTGCCTGG CGGGCGTATC 
AGCTGCCCTT GGAAGGTGCC GCCCCGGGCT ATCACGGAGG CCAATGTGGC TGAGAGGGCA 
GGCCTGCTCC TGGACCAGTA CAGGAAGAAG TCCCGGCTCT TCCGGAGCAA TGTGCTCCTG 
GTGCCGCTGG GCGATGACTT CCGCTACGAC AAGCCCCAGG AGTGGGACGC CCAGTTCTTC 
AACTACCAGC GGCTCTTCGA CTTCCTCAAC AGCAAGCCTG ACCTCCATGT GCAGGCCCAG 
TTTGGCACCC TCTCCGACTA TTTTGACGCT CTATACAAGA GGACAGGGGT GGAGCCGGGA 
GCCCGGCCTC CAGGTTTCCC TGTGCTGAGT GGGGATTTCT TCTCCTACGC GGACCGGGAG 
GACCACTACT GGACAGGCTA TTACACTTCA CGGCCTTTCT ACAAGAACCT GGACCGCGTC 
CTGGAAGCAC ACCTGCGGGG TGCAGAGATT CTGTACAGCC TGGCCGTGGC TCACGCCCGC 
CGCGCTGGAC TGGCCAGCCA GTTCCCACTC TCAAACTTCG CCCTTCTGAC GGACGCTCGG 
CGCACACTGG GGCTCTTCCA GCACCATGAC GCAATCACGG GCACCGCCAA GGAGGCCGTT 
GTGGTGGACT ATGGGGTCAG GCTTCTGCGC TCCCTTGTCA GCCTGAAACA GGTCATCATG 
AACGCAGCTC ACTACCTGGT GCTGGGGGAC AAGAAGGCCT ACCACTTTGA CCCAGAGGTG 
CCCTTCCTGC AGATGGATGA CACCCGCTTA AATCACGACG CCCTTCCAGA GCGCACAGTG 
ATCCAGCTGG AATCCTCACC CAGGTATGTG GTGCTGTTCA ACCCACTGGA GCAGGAGCGG 
CTCAGCGTGG TGTCCCTGCT GGTGAGCTCA CCCCGGGTGC GCGTGCTTTC GGAGGAGGGT 
CAGCCCCTGG CCGTGCAGGT CAGCGCGCAC TGGAGCTCTG CCACCGACAT GGTCCCTGAT 
GTCTATCAGG TGTCTGTGCC TGTCCGCCTG CCAGCGCTGG GTCTTGGCGT GCTGCAGCTG 
CAGCAGGGCC TGGATGGGCC CCGCACGCTG CCCTCCTCTG TGCGCGTCTA CCTACATGGG 
CGGCCGCTGT CCGTCAGCAG GAACGAGGCG TTCCCTCTCC GTGTCATTGA CTCTGGCACC 
AGTGACTTCG CCCTCAGCAA CCGCTACATG CAGGTCTGGT TCTCAGGCCT TACCGGGCTC 
CTCAAGAGCG TCCGAAGGGT GGATGAGGAG CAGGAACAGC GGGTGGACAT GGAGTTCCTC 
GTCTATGGCA CCCGCTCGTC CAAAGACAAG AGTGGAGCCT ACCTCTTCCT GCCTGACGGC 
GAGGCCAAGC CCTACGTCCC CAGGGACCCA CCCGTGCTGC GCGTCACAGA AGGCCCTTTC 
TTCTCAGAGG TGGTTGCCTG CTACGAGCAT GTTCACCAGG TGGTCCGGCT GTATAACCTG 
CCGGGGGTGG AGGGGCTGTC CCTGGATGTG TCGTCCTTGG TGGACATCCG GGACTACGTC 
AACAAGGAGC TGGCCCTGCG CATTCGCACT GACATCGATA GCCAGGGCAT CTTCTTCACA 
GACCTCAACG GCTTTCAGGT GCAGCCCCGA CGGTATCTGA AGAAGCTGCC CCTGCAGGCC 
AACTTCTACC CCATGCCCGT CATGGCCTAC CTCCAGGACG CTCAGAACCG CCTCACGCTG 
CACACAGCCC AGGCCCTGGG TGTCTCCAGC CTCCACAATG GCCAGCTAGA GGTGATCTTG 
GACCGGAGAC TGATGCAGGA CGACAACCGG GGCCTTGGCC AAGGGCTCAA GGACAACAAG 
AGAACCTGCA ACCACTTCCG CCTCCTGTTG GAACGGCGAA CCCTGGGCAG GGAGCCTGGC 
TTTTTCTCCA AACTGGCAGC CATGTTTAGG GGCTTGATCT TTCACAGCAG CAGGAACAGG 
AACCGAGAGG TCCGCGAGGG CTCCTCTGCA AGCTTCCCGT CCCTCCTCAG CCACCTGACC 
TCCATGTACC TGAACACCCC TGTGCTCGCC CTGCCTGTGG CCAGGAGGCA GGCCCCCGGC 
CCCGCTCTGC GCTCTTTTCA CCCTCTGGCC TCCTCACTGC CCTGTGACTT CCACCTGCTC 
AACCTGCGGA CGCTCCAGGC CGAGGAGGAT GGCTTGCCCT CGGCAGAAGC TGCGCTCCTC 
TTACACCGCA AGGGTTTTGA CTGTGGCCTT GAGGCCAAGA ACTTGGGCTT CAACTGCACC 
ACCAGCCAAG GCAAGGTGGC CCTGGGCAGC CTCTTCCATG GCCTGGACGT GGTTTTCCTG 
CAGCCAACCT CCTTGACCCT GCTGTACCCT CTGGCCTCGC CCTCCAATAG CACTGACGTC 
TATGTGGAGC CCATGGAGAT CGCCACCTTC CGCCTCCGCT TGGGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAN2A2 ( XM_012098483.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_011953873.1 Ovis aries mannosidase, alpha, class 2A, member 2 (MAN2A2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OSm27643
Clone ID Related Accession (Same CDS sequence)	XM_012098482.2 , XM_015101896.1 , XM_012098481.2 , XM_012098483.1
Accession Version	XM_012098483.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3528bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries(sheep)
Product	alpha-mannosidase 2x isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014639027.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCTGA AAAAGCAGGT GACAGTGTGT GGGGCTGCTA TCTTCTGCGT GGCTGTCTTC 
TCGCTCTACC TCATGTTGGA CCGAGTACAG CATGATCCTG CTCGACACCA GAACGGTGGG 
AACTTCCCCC GGAGTCAGAT ATCTGTGCTA CAGAACCGCA TTGAACAGCT GGAACAGCTG 
TTGGAGGAGA ACCATGAGAT CATCAGCCAC ATCAAAGACT CTGTGCTGGA GCTGACAGCC 
AACGTGGAGG GCCCACCCGC CCTGCTGCCC TACTATGTGG CCAACGGCTC CTGGGTGGTG 
CCGCCAGAGC CCCGGCCCAG CTTCTTCTCC ATCTCCCCTC AAGACTGCCA GTTTGCTTTG 
GGGGGCCGCG GCGAGAAGCC AGAGCTGCAG ATGCTGACTG TATCAGAGGA GTTACCGTTT 
GACAACGTGG ATGGCGGCGT GTGGAAGCAA GGCTTCGACA TTTCCTACAG CCCGCACGAC 
TGGGACACCG AAGACCTGCA GGTGTTTGTG GTCCCCCACT CTCACAATGA CCCCGGCTGG 
CTCAAGACCT TTGACAAGTA CTACACAGAA CAGACCCAGC ACATCCTCAA CAACATGGTG 
TCCAAGCTGC AGGAGGACCC CCGGCGGCGC TTCATCTGGG CAGAAGTCTC CTTCTTTGCC 
AAGTGGTGGG ACAACATCAG TGCCCAAAAG AGAGCAGCAG TCCGAAGGCT GGTGGGAAAC 
GGGCAGCTGG AGATTGCAAC AGGAGGCTGG GTGATGCCGG ACGAGGCCAA CTCCCACTAT 
TTTGCATTGA TTGACCAGCT CATTGAGGGC CACCAGTGGC TGGAGAAGAA CCTGGGTGCA 
ACTCCGCGCT CGGGCTGGGC GGTGGACCCC TTTGGATACA GCCCCACCAT GCCTTACCTG 
CTGCGCCGTG CCAACCTGAC CAGCATGCTC ATTCAGAGGG TACACTACGC CATCAAGAAG 
CACTTTGCTG CCAGCCACAG CCTGGAGTTC ATGTGGAGGC AGACCTGGGA CTCGGATGCC 
AGCACAGACA TCTTCTGCCA CATGATGCCT TTCTACAGCT ACGATGTCCC CCATACGTGT 
GGCCCGGATC CCAAGATCTG CTGCCAGTTC GATTTCAAGC GCTTGCCTGG CGGGCGTATC 
AGCTGCCCTT GGAAGGTGCC GCCCCGGGCT ATCACGGAGG CCAATGTGGC TGAGAGGGCA 
GGCCTGCTCC TGGACCAGTA CAGGAAGAAG TCCCGGCTCT TCCGGAGCAA TGTGCTCCTG 
GTGCCGCTGG GCGATGACTT CCGCTACGAC AAGCCCCAGG AGTGGGACGC CCAGTTCTTC 
AACTACCAGC GGCTCTTCGA CTTCCTCAAC AGCAAGCCTG ACCTCCATGT GCAGGCCCAG 
TTTGGCACCC TCTCCGACTA TTTTGACGCT CTATACAAGA GGACAGGGGT GGAGCCGGGA 
GCCCGGCCTC CAGGTTTCCC TGTGCTGAGT GGGGATTTCT TCTCCTACGC GGACCGGGAG 
GACCACTACT GGACAGGCTA TTACACTTCA CGGCCTTTCT ACAAGAACCT GGACCGCGTC 
CTGGAAGCAC ACCTGCGGGG TGCAGAGATT CTGTACAGCC TGGCCGTGGC TCACGCCCGC 
CGCGCTGGAC TGGCCAGCCA GTTCCCACTC TCAAACTTCG CCCTTCTGAC GGACGCTCGG 
CGCACACTGG GGCTCTTCCA GCACCATGAC GCAATCACGG GCACCGCCAA GGAGGCCGTT 
GTGGTGGACT ATGGGGTCAG GCTTCTGCGC TCCCTTGTCA GCCTGAAACA GGTCATCATG 
AACGCAGCTC ACTACCTGGT GCTGGGGGAC AAGAAGGCCT ACCACTTTGA CCCAGAGGTG 
CCCTTCCTGC AGATGGATGA CACCCGCTTA AATCACGACG CCCTTCCAGA GCGCACAGTG 
ATCCAGCTGG AATCCTCACC CAGGTATGTG GTGCTGTTCA ACCCACTGGA GCAGGAGCGG 
CTCAGCGTGG TGTCCCTGCT GGTGAGCTCA CCCCGGGTGC GCGTGCTTTC GGAGGAGGGT 
CAGCCCCTGG CCGTGCAGGT CAGCGCGCAC TGGAGCTCTG CCACCGACAT GGTCCCTGAT 
GTCTATCAGG TGTCTGTGCC TGTCCGCCTG CCAGCGCTGG GTCTTGGCGT GCTGCAGCTG 
CAGCAGGGCC TGGATGGGCC CCGCACGCTG CCCTCCTCTG TGCGCGTCTA CCTACATGGG 
CGGCCGCTGT CCGTCAGCAG GAACGAGGCG TTCCCTCTCC GTGTCATTGA CTCTGGCACC 
AGTGACTTCG CCCTCAGCAA CCGCTACATG CAGGTCTGGT TCTCAGGCCT TACCGGGCTC 
CTCAAGAGCG TCCGAAGGGT GGATGAGGAG CAGGAACAGC GGGTGGACAT GGAGTTCCTC 
GTCTATGGCA CCCGCTCGTC CAAAGACAAG AGTGGAGCCT ACCTCTTCCT GCCTGACGGC 
GAGGCCAAGC CCTACGTCCC CAGGGACCCA CCCGTGCTGC GCGTCACAGA AGGCCCTTTC 
TTCTCAGAGG TGGTTGCCTG CTACGAGCAT GTTCACCAGG TGGTCCGGCT GTATAACCTG 
CCGGGGGTGG AGGGGCTGTC CCTGGATGTG TCGTCCTTGG TGGACATCCG GGACTACGTC 
AACAAGGAGC TGGCCCTGCG CATTCGCACT GACATCGATA GCCAGGGCAT CTTCTTCACA 
GACCTCAACG GCTTTCAGGT GCAGCCCCGA CGGTATCTGA AGAAGCTGCC CCTGCAGGCC 
AACTTCTACC CCATGCCCGT CATGGCCTAC CTCCAGGACG CTCAGAACCG CCTCACGCTG 
CACACAGCCC AGGCCCTGGG TGTCTCCAGC CTCCACAATG GCCAGCTAGA GGTGATCTTG 
GACCGGAGAC TGATGCAGGA CGACAACCGG GGCCTTGGCC AAGGGCTCAA GGACAACAAG 
AGAACCTGCA ACCACTTCCG CCTCCTGTTG GAACGGCGAA CCCTGGGCAG GGAGCCTGGC 
TTTTTCTCCA AACTGGCAGC CATGTTTAGG GGCTTGATCT TTCACAGCAG CAGGAACAGG 
AACCGAGAGG TCCGCGAGGG CTCCTCTGCA AGCTTCCCGT CCCTCCTCAG CCACCTGACC 
TCCATGTACC TGAACACCCC TGTGCTCGCC CTGCCTGTGG CCAGGAGGCA GGCCCCCGGC 
CCCGCTCTGC GCTCTTTTCA CCCTCTGGCC TCCTCACTGC CCTGTGACTT CCACCTGCTC 
AACCTGCGGA CGCTCCAGGC CGAGGAGGAT GGCTTGCCCT CGGCAGAAGC TGCGCTCCTC 
TTACACCGCA AGGGTTTTGA CTGTGGCCTT GAGGCCAAGA ACTTGGGCTT CAACTGCACC 
ACCAGCCAAG GCAAGGTGGC CCTGGGCAGC CTCTTCCATG GCCTGGACGT GGTTTTCCTG 
CAGCCAACCT CCTTGACCCT GCTGTACCCT CTGGCCTCGC CCTCCAATAG CACTGACGTC 
TATGTGGAGC CCATGGAGAT CGCCACCTTC CGCCTCCGCT TGGGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011953873.1
CDS	312..3839
Translation	MKLKKQVTVCGAAIFCVAVFSLYLMLDRVQHDPARHQNGGNFPRSQISVLQNRIEQLEQLLEENHEIISHIKDSVLELTANVEGPPALLPYYVANGSWVVPPEPRPSFFSISPQDCQFALGGRGEKPELQMLTVSEELPFDNVDGGVWKQGFDISYSPHDWDTEDLQVFVVPHSHNDPGWLKTFDKYYTEQTQHILNNMVSKLQEDPRRRFIWAEVSFFAKWWDNISAQKRAAVRRLVGNGQLEIATGGWVMPDEANSHYFALIDQLIEGHQWLEKNLGATPRSGWAVDPFGYSPTMPYLLRRANLTSMLIQRVHYAIKKHFAASHSLEFMWRQTWDSDASTDIFCHMMPFYSYDVPHTCGPDPKICCQFDFKRLPGGRISCPWKVPPRAITEANVAERAGLLLDQYRKKSRLFRSNVLLVPLGDDFRYDKPQEWDAQFFNYQRLFDFLNSKPDLHVQAQFGTLSDYFDALYKRTGVEPGARPPGFPVLSGDFFSYADREDHYWTGYYTSRPFYKNLDRVLEAHLRGAEILYSLAVAHARRAGLASQFPLSNFALLTDARRTLGLFQHHDAITGTAKEAVVVDYGVRLLRSLVSLKQVIMNAAHYLVLGDKKAYHFDPEVPFLQMDDTRLNHDALPERTVIQLESSPRYVVLFNPLEQERLSVVSLLVSSPRVRVLSEEGQPLAVQVSAHWSSATDMVPDVYQVSVPVRLPALGLGVLQLQQGLDGPRTLPSSVRVYLHGRPLSVSRNEAFPLRVIDSGTSDFALSNRYMQVWFSGLTGLLKSVRRVDEEQEQRVDMEFLVYGTRSSKDKSGAYLFLPDGEAKPYVPRDPPVLRVTEGPFFSEVVACYEHVHQVVRLYNLPGVEGLSLDVSSLVDIRDYVNKELALRIRTDIDSQGIFFTDLNGFQVQPRRYLKKLPLQANFYPMPVMAYLQDAQNRLTLHTAQALGVSSLHNGQLEVILDRRLMQDDNRGLGQGLKDNKRTCNHFRLLLERRTLGREPGFFSKLAAMFRGLIFHSSRNRNREVREGSSASFPSLLSHLTSMYLNTPVLALPVARRQAPGPALRSFHPLASSLPCDFHLLNLRTLQAEEDGLPSAEAALLLHRKGFDCGLEAKNLGFNCTTSQGKVALGSLFHGLDVVFLQPTSLTLLYPLASPSNSTDVYVEPMEIATFRLRLG

Target ORF information:

RefSeq Version	XM_012098483.1
Organism	Ovis aries(sheep)
Definition	Ovis aries mannosidase, alpha, class 2A, member 2 (MAN2A2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012098483.1

ORF Insert Sequence:

ATGAAGCTGA AAAAGCAGGT GACAGTGTGT GGGGCTGCTA TCTTCTGCGT GGCTGTCTTC 
TCGCTCTACC TCATGTTGGA CCGAGTACAG CATGATCCTG CTCGACACCA GAACGGTGGG 
AACTTCCCCC GGAGTCAGAT ATCTGTGCTA CAGAACCGCA TTGAACAGCT GGAACAGCTG 
TTGGAGGAGA ACCATGAGAT CATCAGCCAC ATCAAAGACT CTGTGCTGGA GCTGACAGCC 
AACGTGGAGG GCCCACCCGC CCTGCTGCCC TACTATGTGG CCAACGGCTC CTGGGTGGTG 
CCGCCAGAGC CCCGGCCCAG CTTCTTCTCC ATCTCCCCTC AAGACTGCCA GTTTGCTTTG 
GGGGGCCGCG GCGAGAAGCC AGAGCTGCAG ATGCTGACTG TATCAGAGGA GTTACCGTTT 
GACAACGTGG ATGGCGGCGT GTGGAAGCAA GGCTTCGACA TTTCCTACAG CCCGCACGAC 
TGGGACACCG AAGACCTGCA GGTGTTTGTG GTCCCCCACT CTCACAATGA CCCCGGCTGG 
CTCAAGACCT TTGACAAGTA CTACACAGAA CAGACCCAGC ACATCCTCAA CAACATGGTG 
TCCAAGCTGC AGGAGGACCC CCGGCGGCGC TTCATCTGGG CAGAAGTCTC CTTCTTTGCC 
AAGTGGTGGG ACAACATCAG TGCCCAAAAG AGAGCAGCAG TCCGAAGGCT GGTGGGAAAC 
GGGCAGCTGG AGATTGCAAC AGGAGGCTGG GTGATGCCGG ACGAGGCCAA CTCCCACTAT 
TTTGCATTGA TTGACCAGCT CATTGAGGGC CACCAGTGGC TGGAGAAGAA CCTGGGTGCA 
ACTCCGCGCT CGGGCTGGGC GGTGGACCCC TTTGGATACA GCCCCACCAT GCCTTACCTG 
CTGCGCCGTG CCAACCTGAC CAGCATGCTC ATTCAGAGGG TACACTACGC CATCAAGAAG 
CACTTTGCTG CCAGCCACAG CCTGGAGTTC ATGTGGAGGC AGACCTGGGA CTCGGATGCC 
AGCACAGACA TCTTCTGCCA CATGATGCCT TTCTACAGCT ACGATGTCCC CCATACGTGT 
GGCCCGGATC CCAAGATCTG CTGCCAGTTC GATTTCAAGC GCTTGCCTGG CGGGCGTATC 
AGCTGCCCTT GGAAGGTGCC GCCCCGGGCT ATCACGGAGG CCAATGTGGC TGAGAGGGCA 
GGCCTGCTCC TGGACCAGTA CAGGAAGAAG TCCCGGCTCT TCCGGAGCAA TGTGCTCCTG 
GTGCCGCTGG GCGATGACTT CCGCTACGAC AAGCCCCAGG AGTGGGACGC CCAGTTCTTC 
AACTACCAGC GGCTCTTCGA CTTCCTCAAC AGCAAGCCTG ACCTCCATGT GCAGGCCCAG 
TTTGGCACCC TCTCCGACTA TTTTGACGCT CTATACAAGA GGACAGGGGT GGAGCCGGGA 
GCCCGGCCTC CAGGTTTCCC TGTGCTGAGT GGGGATTTCT TCTCCTACGC GGACCGGGAG 
GACCACTACT GGACAGGCTA TTACACTTCA CGGCCTTTCT ACAAGAACCT GGACCGCGTC 
CTGGAAGCAC ACCTGCGGGG TGCAGAGATT CTGTACAGCC TGGCCGTGGC TCACGCCCGC 
CGCGCTGGAC TGGCCAGCCA GTTCCCACTC TCAAACTTCG CCCTTCTGAC GGACGCTCGG 
CGCACACTGG GGCTCTTCCA GCACCATGAC GCAATCACGG GCACCGCCAA GGAGGCCGTT 
GTGGTGGACT ATGGGGTCAG GCTTCTGCGC TCCCTTGTCA GCCTGAAACA GGTCATCATG 
AACGCAGCTC ACTACCTGGT GCTGGGGGAC AAGAAGGCCT ACCACTTTGA CCCAGAGGTG 
CCCTTCCTGC AGATGGATGA CACCCGCTTA AATCACGACG CCCTTCCAGA GCGCACAGTG 
ATCCAGCTGG AATCCTCACC CAGGTATGTG GTGCTGTTCA ACCCACTGGA GCAGGAGCGG 
CTCAGCGTGG TGTCCCTGCT GGTGAGCTCA CCCCGGGTGC GCGTGCTTTC GGAGGAGGGT 
CAGCCCCTGG CCGTGCAGGT CAGCGCGCAC TGGAGCTCTG CCACCGACAT GGTCCCTGAT 
GTCTATCAGG TGTCTGTGCC TGTCCGCCTG CCAGCGCTGG GTCTTGGCGT GCTGCAGCTG 
CAGCAGGGCC TGGATGGGCC CCGCACGCTG CCCTCCTCTG TGCGCGTCTA CCTACATGGG 
CGGCCGCTGT CCGTCAGCAG GAACGAGGCG TTCCCTCTCC GTGTCATTGA CTCTGGCACC 
AGTGACTTCG CCCTCAGCAA CCGCTACATG CAGGTCTGGT TCTCAGGCCT TACCGGGCTC 
CTCAAGAGCG TCCGAAGGGT GGATGAGGAG CAGGAACAGC GGGTGGACAT GGAGTTCCTC 
GTCTATGGCA CCCGCTCGTC CAAAGACAAG AGTGGAGCCT ACCTCTTCCT GCCTGACGGC 
GAGGCCAAGC CCTACGTCCC CAGGGACCCA CCCGTGCTGC GCGTCACAGA AGGCCCTTTC 
TTCTCAGAGG TGGTTGCCTG CTACGAGCAT GTTCACCAGG TGGTCCGGCT GTATAACCTG 
CCGGGGGTGG AGGGGCTGTC CCTGGATGTG TCGTCCTTGG TGGACATCCG GGACTACGTC 
AACAAGGAGC TGGCCCTGCG CATTCGCACT GACATCGATA GCCAGGGCAT CTTCTTCACA 
GACCTCAACG GCTTTCAGGT GCAGCCCCGA CGGTATCTGA AGAAGCTGCC CCTGCAGGCC 
AACTTCTACC CCATGCCCGT CATGGCCTAC CTCCAGGACG CTCAGAACCG CCTCACGCTG 
CACACAGCCC AGGCCCTGGG TGTCTCCAGC CTCCACAATG GCCAGCTAGA GGTGATCTTG 
GACCGGAGAC TGATGCAGGA CGACAACCGG GGCCTTGGCC AAGGGCTCAA GGACAACAAG 
AGAACCTGCA ACCACTTCCG CCTCCTGTTG GAACGGCGAA CCCTGGGCAG GGAGCCTGGC 
TTTTTCTCCA AACTGGCAGC CATGTTTAGG GGCTTGATCT TTCACAGCAG CAGGAACAGG 
AACCGAGAGG TCCGCGAGGG CTCCTCTGCA AGCTTCCCGT CCCTCCTCAG CCACCTGACC 
TCCATGTACC TGAACACCCC TGTGCTCGCC CTGCCTGTGG CCAGGAGGCA GGCCCCCGGC 
CCCGCTCTGC GCTCTTTTCA CCCTCTGGCC TCCTCACTGC CCTGTGACTT CCACCTGCTC 
AACCTGCGGA CGCTCCAGGC CGAGGAGGAT GGCTTGCCCT CGGCAGAAGC TGCGCTCCTC 
TTACACCGCA AGGGTTTTGA CTGTGGCCTT GAGGCCAAGA ACTTGGGCTT CAACTGCACC 
ACCAGCCAAG GCAAGGTGGC CCTGGGCAGC CTCTTCCATG GCCTGGACGT GGTTTTCCTG 
CAGCCAACCT CCTTGACCCT GCTGTACCCT CTGGCCTCGC CCTCCAATAG CACTGACGTC 
TATGTGGAGC CCATGGAGAT CGCCACCTTC CGCCTCCGCT TGGGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAN2A2 ( XM_012098484.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_011953874.1 Ovis aries mannosidase, alpha, class 2A, member 2 (MAN2A2), transcript variant X5, mRNA.

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CloneID	OSm27644
Clone ID Related Accession (Same CDS sequence)	XM_012098484.2
Accession Version	XM_012098484.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3402bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries(sheep)
Product	alpha-mannosidase 2x isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014639027.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012098484.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCTGA AAAAGCAGGT GACAGTGTGT GGGGCTGCTA TCTTCTGCGT GGCTGTCTTC 
TCGCTCTACC TCATGTTGGA CCGAGTACAG CATGATCCTG CTCGACACCA GAACGGTGGG 
AACTTCCCCC GGAGTCAGAT ATCTGTGCTA CAGAACCGCA TTGAACAGCT GGAACAGCTG 
TTGGAGGAGA ACCATGAGAT CATCAGCCAC ATCAAAGACT CTGTGCTGGA GCTGACAGCC 
AACGTGGAGG GCCCACCCGC CCTGCTGCCC TACTATGTGG CCAACGGCTC CTGGGTGGTG 
CCGCCAGAGC CCCGGCCCAG CTTCTTCTCC ATCTCCCCTC AAGACTGCCA GTTTGCTTTG 
GGGGGCCGCG GCGAGAAGCC AGAGCTGCAG ATGCTGACTG TATCAGAGGA GTTACCGTTT 
GACAACGTGG ATGGCGGCGT GTGGAAGCAA GGCTTCGACA TTTCCTACAG CCCGCACGAC 
TGGGACACCG AAGACCTGCA GGTGTTTGTG GTCCCCCACT CTCACAATGA CCCCGGCTGG 
CTCAAGACCT TTGACAAGTA CTACACAGAA CAGACCCAGC ACATCCTCAA CAACATGGTG 
TCCAAGCTGC AGGAGGACCC CCGGCGGCGC TTCATCTGGG CAGAAGTCTC CTTCTTTGCC 
AAGTGGTGGG ACAACATCAG TGCCCAAAAG AGAGCAGCAG TCCGAAGGCT GGTGGGAAAC 
GGGCAGCTGG AGATTGCAAC AGGAGGCTGG GTGATGCCGG ACGAGGCCAA CTCCCACTAT 
TTTGCATTGA TTGACCAGCT CATTGAGGGC CACCAGTGGC TGGAGAAGAA CCTGGGTGCA 
ACTCCGCGCT CGGGCTGGGC GGTGGACCCC TTTGGATACA GCCCCACCAT GCCTTACCTG 
CTGCGCCGTG CCAACCTGAC CAGCATGCTC ATTCAGAGGG TACACTACGC CATCAAGAAG 
CACTTTGCTG CCAGCCACAG CCTGGAGTTC ATGTGGAGGC AGACCTGGGA CTCGGATGCC 
AGCACAGACA TCTTCTGCCA CATGATGCCT TTCTACAGCT ACGATGTCCC CCATACGTGT 
GGCCCGGATC CCAAGATCTG CTGCCAGTTC GATTTCAAGC GCTTGCCTGG CGGGCGTATC 
AGCTGCCCTT GGAAGGTGCC GCCCCGGGCT ATCACGGAGG CCAATGTGGC TGAGAGGGCA 
GGCCTGCTCC TGGACCAGTA CAGGAAGAAG TCCCGGCTCT TCCGGAGCAA TGTGCTCCTG 
GTGCCGCTGG GCGATGACTT CCGCTACGAC AAGCCCCAGG AGTGGGACGC CCAGTTCTTC 
AACTACCAGC GGCTCTTCGA CTTCCTCAAC AGCAAGCCTG ACCTCCATGT GCAGGACCAC 
TACTGGACAG GCTATTACAC TTCACGGCCT TTCTACAAGA ACCTGGACCG CGTCCTGGAA 
GCACACCTGC GGGGTGCAGA GATTCTGTAC AGCCTGGCCG TGGCTCACGC CCGCCGCGCT 
GGACTGGCCA GCCAGTTCCC ACTCTCAAAC TTCGCCCTTC TGACGGACGC TCGGCGCACA 
CTGGGGCTCT TCCAGCACCA TGACGCAATC ACGGGCACCG CCAAGGAGGC CGTTGTGGTG 
GACTATGGGG TCAGGCTTCT GCGCTCCCTT GTCAGCCTGA AACAGGTCAT CATGAACGCA 
GCTCACTACC TGGTGCTGGG GGACAAGAAG GCCTACCACT TTGACCCAGA GGTGCCCTTC 
CTGCAGATGG ATGACACCCG CTTAAATCAC GACGCCCTTC CAGAGCGCAC AGTGATCCAG 
CTGGAATCCT CACCCAGGTA TGTGGTGCTG TTCAACCCAC TGGAGCAGGA GCGGCTCAGC 
GTGGTGTCCC TGCTGGTGAG CTCACCCCGG GTGCGCGTGC TTTCGGAGGA GGGTCAGCCC 
CTGGCCGTGC AGGTCAGCGC GCACTGGAGC TCTGCCACCG ACATGGTCCC TGATGTCTAT 
CAGGTGTCTG TGCCTGTCCG CCTGCCAGCG CTGGGTCTTG GCGTGCTGCA GCTGCAGCAG 
GGCCTGGATG GGCCCCGCAC GCTGCCCTCC TCTGTGCGCG TCTACCTACA TGGGCGGCCG 
CTGTCCGTCA GCAGGAACGA GGCGTTCCCT CTCCGTGTCA TTGACTCTGG CACCAGTGAC 
TTCGCCCTCA GCAACCGCTA CATGCAGGTC TGGTTCTCAG GCCTTACCGG GCTCCTCAAG 
AGCGTCCGAA GGGTGGATGA GGAGCAGGAA CAGCGGGTGG ACATGGAGTT CCTCGTCTAT 
GGCACCCGCT CGTCCAAAGA CAAGAGTGGA GCCTACCTCT TCCTGCCTGA CGGCGAGGCC 
AAGCCCTACG TCCCCAGGGA CCCACCCGTG CTGCGCGTCA CAGAAGGCCC TTTCTTCTCA 
GAGGTGGTTG CCTGCTACGA GCATGTTCAC CAGGTGGTCC GGCTGTATAA CCTGCCGGGG 
GTGGAGGGGC TGTCCCTGGA TGTGTCGTCC TTGGTGGACA TCCGGGACTA CGTCAACAAG 
GAGCTGGCCC TGCGCATTCG CACTGACATC GATAGCCAGG GCATCTTCTT CACAGACCTC 
AACGGCTTTC AGGTGCAGCC CCGACGGTAT CTGAAGAAGC TGCCCCTGCA GGCCAACTTC 
TACCCCATGC CCGTCATGGC CTACCTCCAG GACGCTCAGA ACCGCCTCAC GCTGCACACA 
GCCCAGGCCC TGGGTGTCTC CAGCCTCCAC AATGGCCAGC TAGAGGTGAT CTTGGACCGG 
AGACTGATGC AGGACGACAA CCGGGGCCTT GGCCAAGGGC TCAAGGACAA CAAGAGAACC 
TGCAACCACT TCCGCCTCCT GTTGGAACGG CGAACCCTGG GCAGGGAGCC TGGCTTTTTC 
TCCAAACTGG CAGCCATGTT TAGGGGCTTG ATCTTTCACA GCAGCAGGAA CAGGAACCGA 
GAGGTCCGCG AGGGCTCCTC TGCAAGCTTC CCGTCCCTCC TCAGCCACCT GACCTCCATG 
TACCTGAACA CCCCTGTGCT CGCCCTGCCT GTGGCCAGGA GGCAGGCCCC CGGCCCCGCT 
CTGCGCTCTT TTCACCCTCT GGCCTCCTCA CTGCCCTGTG ACTTCCACCT GCTCAACCTG 
CGGACGCTCC AGGCCGAGGA GGATGGCTTG CCCTCGGCAG AAGCTGCGCT CCTCTTACAC 
CGCAAGGGTT TTGACTGTGG CCTTGAGGCC AAGAACTTGG GCTTCAACTG CACCACCAGC 
CAAGGCAAGG TGGCCCTGGG CAGCCTCTTC CATGGCCTGG ACGTGGTTTT CCTGCAGCCA 
ACCTCCTTGA CCCTGCTGTA CCCTCTGGCC TCGCCCTCCA ATAGCACTGA CGTCTATGTG 
GAGCCCATGG AGATCGCCAC CTTCCGCCTC CGCTTGGGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011953874.1
CDS	397..3798
Translation	MKLKKQVTVCGAAIFCVAVFSLYLMLDRVQHDPARHQNGGNFPRSQISVLQNRIEQLEQLLEENHEIISHIKDSVLELTANVEGPPALLPYYVANGSWVVPPEPRPSFFSISPQDCQFALGGRGEKPELQMLTVSEELPFDNVDGGVWKQGFDISYSPHDWDTEDLQVFVVPHSHNDPGWLKTFDKYYTEQTQHILNNMVSKLQEDPRRRFIWAEVSFFAKWWDNISAQKRAAVRRLVGNGQLEIATGGWVMPDEANSHYFALIDQLIEGHQWLEKNLGATPRSGWAVDPFGYSPTMPYLLRRANLTSMLIQRVHYAIKKHFAASHSLEFMWRQTWDSDASTDIFCHMMPFYSYDVPHTCGPDPKICCQFDFKRLPGGRISCPWKVPPRAITEANVAERAGLLLDQYRKKSRLFRSNVLLVPLGDDFRYDKPQEWDAQFFNYQRLFDFLNSKPDLHVQDHYWTGYYTSRPFYKNLDRVLEAHLRGAEILYSLAVAHARRAGLASQFPLSNFALLTDARRTLGLFQHHDAITGTAKEAVVVDYGVRLLRSLVSLKQVIMNAAHYLVLGDKKAYHFDPEVPFLQMDDTRLNHDALPERTVIQLESSPRYVVLFNPLEQERLSVVSLLVSSPRVRVLSEEGQPLAVQVSAHWSSATDMVPDVYQVSVPVRLPALGLGVLQLQQGLDGPRTLPSSVRVYLHGRPLSVSRNEAFPLRVIDSGTSDFALSNRYMQVWFSGLTGLLKSVRRVDEEQEQRVDMEFLVYGTRSSKDKSGAYLFLPDGEAKPYVPRDPPVLRVTEGPFFSEVVACYEHVHQVVRLYNLPGVEGLSLDVSSLVDIRDYVNKELALRIRTDIDSQGIFFTDLNGFQVQPRRYLKKLPLQANFYPMPVMAYLQDAQNRLTLHTAQALGVSSLHNGQLEVILDRRLMQDDNRGLGQGLKDNKRTCNHFRLLLERRTLGREPGFFSKLAAMFRGLIFHSSRNRNREVREGSSASFPSLLSHLTSMYLNTPVLALPVARRQAPGPALRSFHPLASSLPCDFHLLNLRTLQAEEDGLPSAEAALLLHRKGFDCGLEAKNLGFNCTTSQGKVALGSLFHGLDVVFLQPTSLTLLYPLASPSNSTDVYVEPMEIATFRLRLG

Target ORF information:

RefSeq Version	XM_012098484.2
Organism	Ovis aries(sheep)
Definition	Ovis aries mannosidase, alpha, class 2A, member 2 (MAN2A2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012098484.2

ORF Insert Sequence:

ATGAAGCTGA AAAAGCAGGT GACAGTGTGT GGGGCTGCTA TCTTCTGCGT GGCTGTCTTC 
TCGCTCTACC TCATGTTGGA CCGAGTACAG CATGATCCTG CTCGACACCA GAACGGTGGG 
AACTTCCCCC GGAGTCAGAT ATCTGTGCTA CAGAACCGCA TTGAACAGCT GGAACAGCTG 
TTGGAGGAGA ACCATGAGAT CATCAGCCAC ATCAAAGACT CTGTGCTGGA GCTGACAGCC 
AACGTGGAGG GCCCACCCGC CCTGCTGCCC TACTATGTGG CCAACGGCTC CTGGGTGGTG 
CCGCCAGAGC CCCGGCCCAG CTTCTTCTCC ATCTCCCCTC AAGACTGCCA GTTTGCTTTG 
GGGGGCCGCG GCGAGAAGCC AGAGCTGCAG ATGCTGACTG TATCAGAGGA GTTACCGTTT 
GACAACGTGG ATGGCGGCGT GTGGAAGCAA GGCTTCGACA TTTCCTACAG CCCGCACGAC 
TGGGACACCG AAGACCTGCA GGTGTTTGTG GTCCCCCACT CTCACAATGA CCCCGGCTGG 
CTCAAGACCT TTGACAAGTA CTACACAGAA CAGACCCAGC ACATCCTCAA CAACATGGTG 
TCCAAGCTGC AGGAGGACCC CCGGCGGCGC TTCATCTGGG CAGAAGTCTC CTTCTTTGCC 
AAGTGGTGGG ACAACATCAG TGCCCAAAAG AGAGCAGCAG TCCGAAGGCT GGTGGGAAAC 
GGGCAGCTGG AGATTGCAAC AGGAGGCTGG GTGATGCCGG ACGAGGCCAA CTCCCACTAT 
TTTGCATTGA TTGACCAGCT CATTGAGGGC CACCAGTGGC TGGAGAAGAA CCTGGGTGCA 
ACTCCGCGCT CGGGCTGGGC GGTGGACCCC TTTGGATACA GCCCCACCAT GCCTTACCTG 
CTGCGCCGTG CCAACCTGAC CAGCATGCTC ATTCAGAGGG TACACTACGC CATCAAGAAG 
CACTTTGCTG CCAGCCACAG CCTGGAGTTC ATGTGGAGGC AGACCTGGGA CTCGGATGCC 
AGCACAGACA TCTTCTGCCA CATGATGCCT TTCTACAGCT ACGATGTCCC CCATACGTGT 
GGCCCGGATC CCAAGATCTG CTGCCAGTTC GATTTCAAGC GCTTGCCTGG CGGGCGTATC 
AGCTGCCCTT GGAAGGTGCC GCCCCGGGCT ATCACGGAGG CCAATGTGGC TGAGAGGGCA 
GGCCTGCTCC TGGACCAGTA CAGGAAGAAG TCCCGGCTCT TCCGGAGCAA TGTGCTCCTG 
GTGCCGCTGG GCGATGACTT CCGCTACGAC AAGCCCCAGG AGTGGGACGC CCAGTTCTTC 
AACTACCAGC GGCTCTTCGA CTTCCTCAAC AGCAAGCCTG ACCTCCATGT GCAGGACCAC 
TACTGGACAG GCTATTACAC TTCACGGCCT TTCTACAAGA ACCTGGACCG CGTCCTGGAA 
GCACACCTGC GGGGTGCAGA GATTCTGTAC AGCCTGGCCG TGGCTCACGC CCGCCGCGCT 
GGACTGGCCA GCCAGTTCCC ACTCTCAAAC TTCGCCCTTC TGACGGACGC TCGGCGCACA 
CTGGGGCTCT TCCAGCACCA TGACGCAATC ACGGGCACCG CCAAGGAGGC CGTTGTGGTG 
GACTATGGGG TCAGGCTTCT GCGCTCCCTT GTCAGCCTGA AACAGGTCAT CATGAACGCA 
GCTCACTACC TGGTGCTGGG GGACAAGAAG GCCTACCACT TTGACCCAGA GGTGCCCTTC 
CTGCAGATGG ATGACACCCG CTTAAATCAC GACGCCCTTC CAGAGCGCAC AGTGATCCAG 
CTGGAATCCT CACCCAGGTA TGTGGTGCTG TTCAACCCAC TGGAGCAGGA GCGGCTCAGC 
GTGGTGTCCC TGCTGGTGAG CTCACCCCGG GTGCGCGTGC TTTCGGAGGA GGGTCAGCCC 
CTGGCCGTGC AGGTCAGCGC GCACTGGAGC TCTGCCACCG ACATGGTCCC TGATGTCTAT 
CAGGTGTCTG TGCCTGTCCG CCTGCCAGCG CTGGGTCTTG GCGTGCTGCA GCTGCAGCAG 
GGCCTGGATG GGCCCCGCAC GCTGCCCTCC TCTGTGCGCG TCTACCTACA TGGGCGGCCG 
CTGTCCGTCA GCAGGAACGA GGCGTTCCCT CTCCGTGTCA TTGACTCTGG CACCAGTGAC 
TTCGCCCTCA GCAACCGCTA CATGCAGGTC TGGTTCTCAG GCCTTACCGG GCTCCTCAAG 
AGCGTCCGAA GGGTGGATGA GGAGCAGGAA CAGCGGGTGG ACATGGAGTT CCTCGTCTAT 
GGCACCCGCT CGTCCAAAGA CAAGAGTGGA GCCTACCTCT TCCTGCCTGA CGGCGAGGCC 
AAGCCCTACG TCCCCAGGGA CCCACCCGTG CTGCGCGTCA CAGAAGGCCC TTTCTTCTCA 
GAGGTGGTTG CCTGCTACGA GCATGTTCAC CAGGTGGTCC GGCTGTATAA CCTGCCGGGG 
GTGGAGGGGC TGTCCCTGGA TGTGTCGTCC TTGGTGGACA TCCGGGACTA CGTCAACAAG 
GAGCTGGCCC TGCGCATTCG CACTGACATC GATAGCCAGG GCATCTTCTT CACAGACCTC 
AACGGCTTTC AGGTGCAGCC CCGACGGTAT CTGAAGAAGC TGCCCCTGCA GGCCAACTTC 
TACCCCATGC CCGTCATGGC CTACCTCCAG GACGCTCAGA ACCGCCTCAC GCTGCACACA 
GCCCAGGCCC TGGGTGTCTC CAGCCTCCAC AATGGCCAGC TAGAGGTGAT CTTGGACCGG 
AGACTGATGC AGGACGACAA CCGGGGCCTT GGCCAAGGGC TCAAGGACAA CAAGAGAACC 
TGCAACCACT TCCGCCTCCT GTTGGAACGG CGAACCCTGG GCAGGGAGCC TGGCTTTTTC 
TCCAAACTGG CAGCCATGTT TAGGGGCTTG ATCTTTCACA GCAGCAGGAA CAGGAACCGA 
GAGGTCCGCG AGGGCTCCTC TGCAAGCTTC CCGTCCCTCC TCAGCCACCT GACCTCCATG 
TACCTGAACA CCCCTGTGCT CGCCCTGCCT GTGGCCAGGA GGCAGGCCCC CGGCCCCGCT 
CTGCGCTCTT TTCACCCTCT GGCCTCCTCA CTGCCCTGTG ACTTCCACCT GCTCAACCTG 
CGGACGCTCC AGGCCGAGGA GGATGGCTTG CCCTCGGCAG AAGCTGCGCT CCTCTTACAC 
CGCAAGGGTT TTGACTGTGG CCTTGAGGCC AAGAACTTGG GCTTCAACTG CACCACCAGC 
CAAGGCAAGG TGGCCCTGGG CAGCCTCTTC CATGGCCTGG ACGTGGTTTT CCTGCAGCCA 
ACCTCCTTGA CCCTGCTGTA CCCTCTGGCC TCGCCCTCCA ATAGCACTGA CGTCTATGTG 
GAGCCCATGG AGATCGCCAC CTTCCGCCTC CGCTTGGGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAN2A2 ( XM_012098485.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_011953875.1 Ovis aries mannosidase, alpha, class 2A, member 2 (MAN2A2), transcript variant X6, mRNA.

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CloneID	OSm27645
Clone ID Related Accession (Same CDS sequence)	XM_012098485.2
Accession Version	XM_012098485.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3327bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries(sheep)
Product	alpha-mannosidase 2x isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014639027.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012098485.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCTGA AAAAGCAGGT GACAGTGTGT GGGGCTGCTA TCTTCTGCGT GGCTGTCTTC 
TCGCTCTACC TCATGTTGGA CCGAGTACAG CATGATCCTG CTCGACACCA GAACGGTGGG 
AACTTCCCCC GGAGTCAGAT ATCTGTGCTA CAGAACCGCA TTGAACAGCT GGAACAGCTG 
TTGGAGGAGA ACCATGAGAT CATCAGCCAC ATCAAAGACT CTGTGCTGGA GCTGACAGCC 
AACGTGGAGG GCCCACCCGC CCTGCTGCCC TACTATGTGG CCAACGGCTC CTGGGTGGTG 
CCGCCAGAGC CCCGGCCCAG CTTCTTCTCC ATCTCCCCTC AAGACTGCCA GTTTGCTTTG 
GGGGGCCGCG GCGAGAAGCC AGAGCTGCAG ATGCTGACTG TATCAGAGGA GTTACCGTTT 
GACAACGTGG ATGGCGGCGT GTGGAAGCAA GGCTTCGACA TTTCCTACAG CCCGCACGAC 
TGGGACACCG AAGACCTGCA GGTGTTTGTG GTCCCCCACT CTCACAATGA CCCCGGCTGG 
CTCAAGACCT TTGACAAGTA CTACACAGAA CAGACCCAGC ACATCCTCAA CAACATGGTG 
TCCAAGCTGC AGGAGGACCC CCGGCGGCGC TTCATCTGGG CAGAAGTCTC CTTCTTTGCC 
AAGTGGTGGG ACAACATCAG TGCCCAAAAG AGAGCAGCAG TCCGAAGGCT GGTGGGAAAC 
GGGCAGCTGG AGATTGCAAC AGGAGGCTGG GTGATGCCGG ACGAGGCCAA CTCCCACTAT 
TTTGCATTGA TTGACCAGCT CATTGAGGGC CACCAGTGGC TGGAGAAGAA CCTGGGTGCA 
ACTCCGCGCT CGGGCTGGGC GGTGGACCCC TTTGGATACA GCCCCACCAT GCCTTACCTG 
CTGCGCCGTG CCAACCTGAC CAGCATGCTC ATTCAGAGGG TACACTACGC CATCAAGAAG 
CACTTTGCTG CCAGCCACAG CCTGGAGTTC ATGTGGAGGC AGACCTGGGA CTCGGATGCC 
AGCACAGACA TCTTCTGCCA CATGATGCCT TTCTACAGCT ACGATGTCCC CCATACGTGT 
GGCCCGGATC CCAAGATCTG CTGCCAGTTC GATTTCAAGC GCTTGCCTGG CGGGCGTATC 
AGCTGCCCTT GGAAGGTGCC GCCCCGGGCT ATCACGGAGG CCAATGTGGC TGAGAGGGCA 
GGCCTGCTCC TGGACCAGTA CAGGAAGAAG TCCCGGCTCT TCCGGAGCAA TGTGCTCCTG 
GTGCCGCTGG GCGATGACTT CCGCTACGAC AAGCCCCAGG AGTGGGACGC CCAGTTCTTC 
AACTACCAGC GGCTCTTCGA CTTCCTCAAC AGCAAGCCTG ACCTCCATGT GCAGGACCAC 
TACTGGACAG GCTATTACAC TTCACGGCCT TTCTACAAGA ACCTGGACCG CGTCCTGGAA 
GCACACCTGC GGGGTGCAGA GATTCTGTAC AGCCTGGCCG TGGCTCACGC CCGCCGCGCT 
GGACTGGCCA GCCAGTTCCC ACTCTCAAAC TTCGCCCTTC TGACGGACGC TCGGCGCACA 
CTGGGGCTCT TCCAGCACCA TGACGCAATC ACGGGCACCG CCAAGGAGGC CGTTGTGGTG 
GACTATGGGG TCAGGCTTCT GCGCTCCCTT GTCAGCCTGA AACAGGTCAT CATGAACGCA 
GCTCACTACC TGGTGCTGGG GGACAAGAAG GCCTACCACT TTGACCCAGA GGTGCCCTTC 
CTGCAGATGG ATGACACCCG CTTAAATCAC GACGCCCTTC CAGAGCGCAC AGTGATCCAG 
CTGGAATCCT CACCCAGGTA TGTGGTGCTG TTCAACCCAC TGGAGCAGGA GCGGCTCAGC 
GTGGTGTCCC TGCTGGTGAG CTCACCCCGG GTGCGCGTGC TTTCGGAGGA GGGTCAGCCC 
CTGGCCGTGC AGGTCAGCGC GCACTGGAGC TCTGCCACCG ACATGGTCCC TGATGTCTAT 
CAGGTGTCTG TGCCTGTCCG CCTGCCAGCG CTGGGTCTTG GCGTGCTGCA GCTGCAGCAG 
GGCCTGGATG GGCCCCGCAC GCTGCCCTCC TCTGTGCGCG TCTACCTACA TGGGCGGCCG 
CTGTCCGTCA GCAGGAACGA GGCGTTCCCT CTCCGTGTCA TTGACTCTGG CACCAGTGAC 
TTCGCCCTCA GCAACCGCTA CATGCAGGTC TGGTTCTCAG GCCTTACCGG GCTCCTCAAG 
AGCGTCCGAA GGGTGGATGA GGAGCAGGAA CAGCGGGTGG ACATGGAGTT CCTCGTCTAT 
GGCACCCGCT CGTCCAAAGA CAAGAGTGGA GCCTACCTCT TCCTGCCTGA CGGCGAGGCC 
AAGCCCTACG TCCCCAGGGA CCCACCCGTG CTGCGCGTCA CAGAAGGCCC TTTCTTCTCA 
GAGGTGGTTG CCTGCTACGA GCATGTTCAC CAGGTGGTCC GGCTGTATAA CCTGCCGGGG 
GTGGAGGGGC TGTCCCTGGA TGTGTCGTCC TTGGTGGACA TCCGGGACTA CGTCAACAAG 
GAGCTGGCCC TGCGCATTCG CACTGACATC GATAGCCAGG GCATCTTCTT CACAGACCTC 
AACGGCTTTC AGGTGCAGCC CCGACGGTAT CTGAAGAAGC TGCCCCTGCA GGCCAACTTC 
TACCCCATGC CCGTCATGGC CTACCTCCAG GACGCTCAGA ACCGCCTCAC GCTGCACACA 
GCCCAGGCCC TGGGTGTCTC CAGCCTCCAC AATGGCCAGC TAGAGGTGAT CTTGGACCGG 
AGACTGATGC AGGACGACAA CCGGGGCCTT GGCCAAGGGC TCAAGGACAA CAAGAGAACC 
TGCAACCACT TCCGCCTCCT GTTGGAACGG CGAACCCTGG GCAGGGAGGT CCGCGAGGGC 
TCCTCTGCAA GCTTCCCGTC CCTCCTCAGC CACCTGACCT CCATGTACCT GAACACCCCT 
GTGCTCGCCC TGCCTGTGGC CAGGAGGCAG GCCCCCGGCC CCGCTCTGCG CTCTTTTCAC 
CCTCTGGCCT CCTCACTGCC CTGTGACTTC CACCTGCTCA ACCTGCGGAC GCTCCAGGCC 
GAGGAGGATG GCTTGCCCTC GGCAGAAGCT GCGCTCCTCT TACACCGCAA GGGTTTTGAC 
TGTGGCCTTG AGGCCAAGAA CTTGGGCTTC AACTGCACCA CCAGCCAAGG CAAGGTGGCC 
CTGGGCAGCC TCTTCCATGG CCTGGACGTG GTTTTCCTGC AGCCAACCTC CTTGACCCTG 
CTGTACCCTC TGGCCTCGCC CTCCAATAGC ACTGACGTCT ATGTGGAGCC CATGGAGATC 
GCCACCTTCC GCCTCCGCTT GGGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011953875.1
CDS	395..3721
Translation	MKLKKQVTVCGAAIFCVAVFSLYLMLDRVQHDPARHQNGGNFPRSQISVLQNRIEQLEQLLEENHEIISHIKDSVLELTANVEGPPALLPYYVANGSWVVPPEPRPSFFSISPQDCQFALGGRGEKPELQMLTVSEELPFDNVDGGVWKQGFDISYSPHDWDTEDLQVFVVPHSHNDPGWLKTFDKYYTEQTQHILNNMVSKLQEDPRRRFIWAEVSFFAKWWDNISAQKRAAVRRLVGNGQLEIATGGWVMPDEANSHYFALIDQLIEGHQWLEKNLGATPRSGWAVDPFGYSPTMPYLLRRANLTSMLIQRVHYAIKKHFAASHSLEFMWRQTWDSDASTDIFCHMMPFYSYDVPHTCGPDPKICCQFDFKRLPGGRISCPWKVPPRAITEANVAERAGLLLDQYRKKSRLFRSNVLLVPLGDDFRYDKPQEWDAQFFNYQRLFDFLNSKPDLHVQDHYWTGYYTSRPFYKNLDRVLEAHLRGAEILYSLAVAHARRAGLASQFPLSNFALLTDARRTLGLFQHHDAITGTAKEAVVVDYGVRLLRSLVSLKQVIMNAAHYLVLGDKKAYHFDPEVPFLQMDDTRLNHDALPERTVIQLESSPRYVVLFNPLEQERLSVVSLLVSSPRVRVLSEEGQPLAVQVSAHWSSATDMVPDVYQVSVPVRLPALGLGVLQLQQGLDGPRTLPSSVRVYLHGRPLSVSRNEAFPLRVIDSGTSDFALSNRYMQVWFSGLTGLLKSVRRVDEEQEQRVDMEFLVYGTRSSKDKSGAYLFLPDGEAKPYVPRDPPVLRVTEGPFFSEVVACYEHVHQVVRLYNLPGVEGLSLDVSSLVDIRDYVNKELALRIRTDIDSQGIFFTDLNGFQVQPRRYLKKLPLQANFYPMPVMAYLQDAQNRLTLHTAQALGVSSLHNGQLEVILDRRLMQDDNRGLGQGLKDNKRTCNHFRLLLERRTLGREVREGSSASFPSLLSHLTSMYLNTPVLALPVARRQAPGPALRSFHPLASSLPCDFHLLNLRTLQAEEDGLPSAEAALLLHRKGFDCGLEAKNLGFNCTTSQGKVALGSLFHGLDVVFLQPTSLTLLYPLASPSNSTDVYVEPMEIATFRLRLG

Target ORF information:

RefSeq Version	XM_012098485.2
Organism	Ovis aries(sheep)
Definition	Ovis aries mannosidase, alpha, class 2A, member 2 (MAN2A2), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012098485.2

ORF Insert Sequence:

ATGAAGCTGA AAAAGCAGGT GACAGTGTGT GGGGCTGCTA TCTTCTGCGT GGCTGTCTTC 
TCGCTCTACC TCATGTTGGA CCGAGTACAG CATGATCCTG CTCGACACCA GAACGGTGGG 
AACTTCCCCC GGAGTCAGAT ATCTGTGCTA CAGAACCGCA TTGAACAGCT GGAACAGCTG 
TTGGAGGAGA ACCATGAGAT CATCAGCCAC ATCAAAGACT CTGTGCTGGA GCTGACAGCC 
AACGTGGAGG GCCCACCCGC CCTGCTGCCC TACTATGTGG CCAACGGCTC CTGGGTGGTG 
CCGCCAGAGC CCCGGCCCAG CTTCTTCTCC ATCTCCCCTC AAGACTGCCA GTTTGCTTTG 
GGGGGCCGCG GCGAGAAGCC AGAGCTGCAG ATGCTGACTG TATCAGAGGA GTTACCGTTT 
GACAACGTGG ATGGCGGCGT GTGGAAGCAA GGCTTCGACA TTTCCTACAG CCCGCACGAC 
TGGGACACCG AAGACCTGCA GGTGTTTGTG GTCCCCCACT CTCACAATGA CCCCGGCTGG 
CTCAAGACCT TTGACAAGTA CTACACAGAA CAGACCCAGC ACATCCTCAA CAACATGGTG 
TCCAAGCTGC AGGAGGACCC CCGGCGGCGC TTCATCTGGG CAGAAGTCTC CTTCTTTGCC 
AAGTGGTGGG ACAACATCAG TGCCCAAAAG AGAGCAGCAG TCCGAAGGCT GGTGGGAAAC 
GGGCAGCTGG AGATTGCAAC AGGAGGCTGG GTGATGCCGG ACGAGGCCAA CTCCCACTAT 
TTTGCATTGA TTGACCAGCT CATTGAGGGC CACCAGTGGC TGGAGAAGAA CCTGGGTGCA 
ACTCCGCGCT CGGGCTGGGC GGTGGACCCC TTTGGATACA GCCCCACCAT GCCTTACCTG 
CTGCGCCGTG CCAACCTGAC CAGCATGCTC ATTCAGAGGG TACACTACGC CATCAAGAAG 
CACTTTGCTG CCAGCCACAG CCTGGAGTTC ATGTGGAGGC AGACCTGGGA CTCGGATGCC 
AGCACAGACA TCTTCTGCCA CATGATGCCT TTCTACAGCT ACGATGTCCC CCATACGTGT 
GGCCCGGATC CCAAGATCTG CTGCCAGTTC GATTTCAAGC GCTTGCCTGG CGGGCGTATC 
AGCTGCCCTT GGAAGGTGCC GCCCCGGGCT ATCACGGAGG CCAATGTGGC TGAGAGGGCA 
GGCCTGCTCC TGGACCAGTA CAGGAAGAAG TCCCGGCTCT TCCGGAGCAA TGTGCTCCTG 
GTGCCGCTGG GCGATGACTT CCGCTACGAC AAGCCCCAGG AGTGGGACGC CCAGTTCTTC 
AACTACCAGC GGCTCTTCGA CTTCCTCAAC AGCAAGCCTG ACCTCCATGT GCAGGACCAC 
TACTGGACAG GCTATTACAC TTCACGGCCT TTCTACAAGA ACCTGGACCG CGTCCTGGAA 
GCACACCTGC GGGGTGCAGA GATTCTGTAC AGCCTGGCCG TGGCTCACGC CCGCCGCGCT 
GGACTGGCCA GCCAGTTCCC ACTCTCAAAC TTCGCCCTTC TGACGGACGC TCGGCGCACA 
CTGGGGCTCT TCCAGCACCA TGACGCAATC ACGGGCACCG CCAAGGAGGC CGTTGTGGTG 
GACTATGGGG TCAGGCTTCT GCGCTCCCTT GTCAGCCTGA AACAGGTCAT CATGAACGCA 
GCTCACTACC TGGTGCTGGG GGACAAGAAG GCCTACCACT TTGACCCAGA GGTGCCCTTC 
CTGCAGATGG ATGACACCCG CTTAAATCAC GACGCCCTTC CAGAGCGCAC AGTGATCCAG 
CTGGAATCCT CACCCAGGTA TGTGGTGCTG TTCAACCCAC TGGAGCAGGA GCGGCTCAGC 
GTGGTGTCCC TGCTGGTGAG CTCACCCCGG GTGCGCGTGC TTTCGGAGGA GGGTCAGCCC 
CTGGCCGTGC AGGTCAGCGC GCACTGGAGC TCTGCCACCG ACATGGTCCC TGATGTCTAT 
CAGGTGTCTG TGCCTGTCCG CCTGCCAGCG CTGGGTCTTG GCGTGCTGCA GCTGCAGCAG 
GGCCTGGATG GGCCCCGCAC GCTGCCCTCC TCTGTGCGCG TCTACCTACA TGGGCGGCCG 
CTGTCCGTCA GCAGGAACGA GGCGTTCCCT CTCCGTGTCA TTGACTCTGG CACCAGTGAC 
TTCGCCCTCA GCAACCGCTA CATGCAGGTC TGGTTCTCAG GCCTTACCGG GCTCCTCAAG 
AGCGTCCGAA GGGTGGATGA GGAGCAGGAA CAGCGGGTGG ACATGGAGTT CCTCGTCTAT 
GGCACCCGCT CGTCCAAAGA CAAGAGTGGA GCCTACCTCT TCCTGCCTGA CGGCGAGGCC 
AAGCCCTACG TCCCCAGGGA CCCACCCGTG CTGCGCGTCA CAGAAGGCCC TTTCTTCTCA 
GAGGTGGTTG CCTGCTACGA GCATGTTCAC CAGGTGGTCC GGCTGTATAA CCTGCCGGGG 
GTGGAGGGGC TGTCCCTGGA TGTGTCGTCC TTGGTGGACA TCCGGGACTA CGTCAACAAG 
GAGCTGGCCC TGCGCATTCG CACTGACATC GATAGCCAGG GCATCTTCTT CACAGACCTC 
AACGGCTTTC AGGTGCAGCC CCGACGGTAT CTGAAGAAGC TGCCCCTGCA GGCCAACTTC 
TACCCCATGC CCGTCATGGC CTACCTCCAG GACGCTCAGA ACCGCCTCAC GCTGCACACA 
GCCCAGGCCC TGGGTGTCTC CAGCCTCCAC AATGGCCAGC TAGAGGTGAT CTTGGACCGG 
AGACTGATGC AGGACGACAA CCGGGGCCTT GGCCAAGGGC TCAAGGACAA CAAGAGAACC 
TGCAACCACT TCCGCCTCCT GTTGGAACGG CGAACCCTGG GCAGGGAGGT CCGCGAGGGC 
TCCTCTGCAA GCTTCCCGTC CCTCCTCAGC CACCTGACCT CCATGTACCT GAACACCCCT 
GTGCTCGCCC TGCCTGTGGC CAGGAGGCAG GCCCCCGGCC CCGCTCTGCG CTCTTTTCAC 
CCTCTGGCCT CCTCACTGCC CTGTGACTTC CACCTGCTCA ACCTGCGGAC GCTCCAGGCC 
GAGGAGGATG GCTTGCCCTC GGCAGAAGCT GCGCTCCTCT TACACCGCAA GGGTTTTGAC 
TGTGGCCTTG AGGCCAAGAA CTTGGGCTTC AACTGCACCA CCAGCCAAGG CAAGGTGGCC 
CTGGGCAGCC TCTTCCATGG CCTGGACGTG GTTTTCCTGC AGCCAACCTC CTTGACCCTG 
CTGTACCCTC TGGCCTCGCC CTCCAATAGC ACTGACGTCT ATGTGGAGCC CATGGAGATC 
GCCACCTTCC GCCTCCGCTT GGGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	MAN2A2
Entrez Gene ID	101106798
Full Name	mannosidase alpha class 2A member 2
Gene Type	protein-coding
Organism	Ovis aries(sheep)

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

MAN2A2 cDNA ORF clone, Ovis aries(sheep)

Gene

Clones

gRNAs

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