LOC4349420 cDNA ORF clone, Oryza sativa Japonica Group(Japanese rice)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC4349420
Entrez Gene ID	4349420
Full Name	E3 ubiquitin-protein ligase BRE1-like 2
Synonyms	BRE1B,OsJ_031219
Gene Type	protein-coding
Organism	Oryza sativa Japonica Group(Japanese rice)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015759100.1	XP_015614586.1	E3 ubiquitin-protein ligase BRE1-like 2
XM_026020810.1	XP_025876595.1	E3 ubiquitin-protein ligase BRE1-like 2 isoform X1
XM_015759100.2	XP_015614586.1	E3 ubiquitin-protein ligase BRE1-like 2 isoform X1
XM_026020811.1	XP_025876596.1	E3 ubiquitin-protein ligase BRE1-like 2 isoform X2

Pathways from BioSystems

Homologies

Arabidopsis thaliana (thale cress)	HUB2	NP_564680.4
Oryza sativa (rice)	Os10g0565600	NP_001065421.1

Gene Ontology

The following LOC4349420 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC4349420 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OOa21059	XM_015759100.2 Latest version!	Oryza sativa Japonica Group E3 ubiquitin-protein ligase BRE1-like 2 (LOC4349420), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OOa21059	XM_026020810.1 Latest version!	Oryza sativa Japonica Group E3 ubiquitin-protein ligase BRE1-like 2 (LOC4349420), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OOa44694	XM_026020811.1 Latest version!	Oryza sativa Japonica Group E3 ubiquitin-protein ligase BRE1-like 2 (LOC4349420), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
OOa21059	XM_015759100.1	Oryza sativa Japonica Group E3 ubiquitin-protein ligase BRE1-like 2 (LOC4349420), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC4349420 ( XM_015759100.1 ) cDNA ORF clone, Oryza sativa Japonica Group(Japanese rice) -> XP_015614586.1 Oryza sativa Japonica Group E3 ubiquitin-protein ligase BRE1-like 2 (LOC4349420), mRNA.

Price & Availability↑

CloneID	OOa21059
Clone ID Related Accession (Same CDS sequence)	XM_015759100.1 , XM_026020810.1 , XM_015759100.2
Accession Version	XM_015759100.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2535bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-02-29
Organism	Oryza sativa Japonica Group(Japanese rice)
Product	E3 ubiquitin-protein ligase BRE1-like 2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015379183.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oryza sativa Japonica Group Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGCCG CAGCTCTTCA GTATGAGAAC CAGAAGCTGG TGCAGCAATT GGAGGCACAG 
AAGTCCAAAA TGCGTGCATT GGAAGGCAAG TTCAAGGAGC TGAGGGATGA GCAATGTTCT 
TACGACAATA CATTGATTTG TTTGAATAAA ATGTGGAATC AGCTGATTGA TGATTTAGTG 
CTGCTTGGGG TTCGAGCTGG TGGGGATTTA AACGGTTTGC AAGCGCTTGA TCATGAAGAG 
ATGTCAGAAG AATCTCTTGA GTCATGTCCT TCTGAGGAGA TATTTCTCTT CAGACTCTTG 
AATTCCAGGA ATTTCAGAAA TAATGATGAC AGTAGCCTGT CAAAATTAGT TGAGGAAGCT 
CTTGCCTTGC GCTATTCAAC AACAGTTACT CTGATGAAGT CCCTGCAAGA GGCTTTTGCT 
GTACAGCAGG CTAGAAGTGA ATCTTTATCA TTAGCTTTAA ATGGACAGAA CTCCAGTGAA 
GACGTTATTG TGGCCCTTGA AAACCATAAT GATTACTTGA AAGAAGTGGT TGACAATTTA 
CGTCAAGCTG TGTCTATCAT CAACAGGAAG CACGAAAAGT ATCTGGATGA GATTGAGGCT 
TTCAAAAACA ATCAATCAAG AGAACTACAT GAAGTAAAGT GTCTTTCAGG TGAGCTAGAG 
GAAAGCATGG CAGAGCTTGA GGAAAGCCGT CGAAAGTTGG CTGTTCTCCA GTTGCAAACG 
GGTGGAGGCT CACTGATGAA CACATCTGCT CCTAACGGTG TGAACGGTAG TGTTTCAACT 
GATAAATCTT CAGACAAGGG TATGGGCTGG CGAGATCTGA AAGATGCTGT TGAGGAGGCT 
AAGACTCTTG CTGCAAACCG CCTATTTGAA CTCCATGAGA CTCAAGAGGA TAATCTGATA 
CTATCCAAGC AGTTGGAAGA TATTCAGGAT CAATTAAAAG ATGAGAACTA TATTGTTACA 
TCAAAACCAT ATACGATTCT CAGTGACCAG TTACACCATC TGAATGCTGA GATAGAGCGA 
TACAGGGGGT TAGTTGAAGT GCTACAGAAT GAAAAGGACC AGCTCATGCA AAAGGAAGAA 
GAAATGCTTG CAAAGGCAGA ATCAGTGGAC GCTGTTCAAC AATCCATTAC CACTTACAAG 
GCCAAAATTG AGGACCTGGA ACATGAAATC CAGAAACTTA TGGCTGAAAA GAATGATCTT 
GAGATCAAGG CTGAGGAAGC CTTGCAAGAT TCAGGGAAAA AAGACTTCAA GGACGAGATT 
CATGTTATGG CTGCATCACT CTCCAAAGAG ATGGAATTGT TGGACAATCA AATGAATAGA 
TCCAAGGATG CGGCTTCTGA AGCACTTGCA CTACGTGAGG AAGCTGACTA TTTAAGAACT 
TTGCTAGCTA AGAAGATTGA TGAACAAAAG GAGATATCTG ATAGATACAA CACACAAGTC 
ACCGAGATCA AATCCCTCAA AGCATTGATT GAGACACTGG ACCAGGAAAA GCAGGAGTTA 
CAATTTATAG TGGATATGCT CGGTAAAGAA TGTTCTGAGT CGAGGGCAAT TTCAGAGATT 
GAAGAATCAG AAAACCGAGC TCGCAAGCAA GCTGAATATT TGAGAAAATG TTTAGAAGAG 
CATAATCTTG AACTTCGAGT AAAAGCAGCA AATGAAGCGG AAACTGCATG CCAACAAAGG 
CTTTCAATTG CTGAAGCAGA ACTGGAAGAC TTAAGGGCCA AAGTGGATGC ATCGGAAAGA 
GATGTTATGA AACTCAAGGA GTCAATAAGA ATTAAAGAAG CTGAAGTAGA TGGCCATATT 
TCTGAAATTG AGACAATTGG TCAAGCATAT GAAGACATGC AAACCCAAAA TCAGCACCTT 
CTTCAACAGG TTGCTGATAG AGATGATTTT AACATAAAGC TAGTATCAGA TAGTGTAAAG 
ATGAAGCAAG CCTATGGTTC CCTTCTTGCT GAAAAGAATA TGCTACAGAA GCAACTTCAG 
CATGTCAACA GTTCACTGGA GTCATCTAAA CTAAAAATCA CCAGTGGTGA AGAGCAGATG 
AAGACCTATG TAGCACAAGC TATGAAATCT TCTTCAGAGA ATAGGCATCT TGCAATTAGC 
CTTGAAAGGA CTATGCTGGA GGTATCAGAT GCAGAGAAGG AGCTCAAGTG GCTTCGGTCC 
GCTACTGGAT CTGCTGAGAA AGAATATGAG ATAAATCAGA AAAAAATAGC TGAGCTAAAA 
ATGGAGCTAG AGCGTGAGAG AAATGAGAGG ATAAAGCTTG AGGAAGAATA CGAAGAGGTG 
AAAAACGAAG TCAGTGAGCT AACCTCAGAA ACTGAAGAGA CTACTATACA GAAGCTCCAA 
GATGAAATAA AAGAATGCAA GGCTATTCTC AAGTGTGGGG TGTGCTTCGA CCGGCCGAAA 
GAGGTCGTGA TCACCAAATG CTTCCACTTA TTCTGCTCGC CATGTATCCA GAGGAACCTT 
GAGATCCGCC ACCGCAAATG CCCAGGTTGT GGCACCCCCT TTGGACAGAG TGACGTTCGG 
GAGGTGAAGA TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015614586.1
CDS	153..2687
Translation	MDAAALQYENQKLVQQLEAQKSKMRALEGKFKELRDEQCSYDNTLICLNKMWNQLIDDLVLLGVRAGGDLNGLQALDHEEMSEESLESCPSEEIFLFRLLNSRNFRNNDDSSLSKLVEEALALRYSTTVTLMKSLQEAFAVQQARSESLSLALNGQNSSEDVIVALENHNDYLKEVVDNLRQAVSIINRKHEKYLDEIEAFKNNQSRELHEVKCLSGELEESMAELEESRRKLAVLQLQTGGGSLMNTSAPNGVNGSVSTDKSSDKGMGWRDLKDAVEEAKTLAANRLFELHETQEDNLILSKQLEDIQDQLKDENYIVTSKPYTILSDQLHHLNAEIERYRGLVEVLQNEKDQLMQKEEEMLAKAESVDAVQQSITTYKAKIEDLEHEIQKLMAEKNDLEIKAEEALQDSGKKDFKDEIHVMAASLSKEMELLDNQMNRSKDAASEALALREEADYLRTLLAKKIDEQKEISDRYNTQVTEIKSLKALIETLDQEKQELQFIVDMLGKECSESRAISEIEESENRARKQAEYLRKCLEEHNLELRVKAANEAETACQQRLSIAEAELEDLRAKVDASERDVMKLKESIRIKEAEVDGHISEIETIGQAYEDMQTQNQHLLQQVADRDDFNIKLVSDSVKMKQAYGSLLAEKNMLQKQLQHVNSSLESSKLKITSGEEQMKTYVAQAMKSSSENRHLAISLERTMLEVSDAEKELKWLRSATGSAEKEYEINQKKIAELKMELERERNERIKLEEEYEEVKNEVSELTSETEETTIQKLQDEIKECKAILKCGVCFDRPKEVVITKCFHLFCSPCIQRNLEIRHRKCPGCGTPFGQSDVREVKI

Target ORF information:

RefSeq Version	XM_015759100.1
Organism	Oryza sativa Japonica Group(Japanese rice)
Definition	Oryza sativa Japonica Group E3 ubiquitin-protein ligase BRE1-like 2 (LOC4349420), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015759100.1

ORF Insert Sequence:

ATGGATGCCG CAGCTCTTCA GTATGAGAAC CAGAAGCTGG TGCAGCAATT GGAGGCACAG 
AAGTCCAAAA TGCGTGCATT GGAAGGCAAG TTCAAGGAGC TGAGGGATGA GCAATGTTCT 
TACGACAATA CATTGATTTG TTTGAATAAA ATGTGGAATC AGCTGATTGA TGATTTAGTG 
CTGCTTGGGG TTCGAGCTGG TGGGGATTTA AACGGTTTGC AAGCGCTTGA TCATGAAGAG 
ATGTCAGAAG AATCTCTTGA GTCATGTCCT TCTGAGGAGA TATTTCTCTT CAGACTCTTG 
AATTCCAGGA ATTTCAGAAA TAATGATGAC AGTAGCCTGT CAAAATTAGT TGAGGAAGCT 
CTTGCCTTGC GCTATTCAAC AACAGTTACT CTGATGAAGT CCCTGCAAGA GGCTTTTGCT 
GTACAGCAGG CTAGAAGTGA ATCTTTATCA TTAGCTTTAA ATGGACAGAA CTCCAGTGAA 
GACGTTATTG TGGCCCTTGA AAACCATAAT GATTACTTGA AAGAAGTGGT TGACAATTTA 
CGTCAAGCTG TGTCTATCAT CAACAGGAAG CACGAAAAGT ATCTGGATGA GATTGAGGCT 
TTCAAAAACA ATCAATCAAG AGAACTACAT GAAGTAAAGT GTCTTTCAGG TGAGCTAGAG 
GAAAGCATGG CAGAGCTTGA GGAAAGCCGT CGAAAGTTGG CTGTTCTCCA GTTGCAAACG 
GGTGGAGGCT CACTGATGAA CACATCTGCT CCTAACGGTG TGAACGGTAG TGTTTCAACT 
GATAAATCTT CAGACAAGGG TATGGGCTGG CGAGATCTGA AAGATGCTGT TGAGGAGGCT 
AAGACTCTTG CTGCAAACCG CCTATTTGAA CTCCATGAGA CTCAAGAGGA TAATCTGATA 
CTATCCAAGC AGTTGGAAGA TATTCAGGAT CAATTAAAAG ATGAGAACTA TATTGTTACA 
TCAAAACCAT ATACGATTCT CAGTGACCAG TTACACCATC TGAATGCTGA GATAGAGCGA 
TACAGGGGGT TAGTTGAAGT GCTACAGAAT GAAAAGGACC AGCTCATGCA AAAGGAAGAA 
GAAATGCTTG CAAAGGCAGA ATCAGTGGAC GCTGTTCAAC AATCCATTAC CACTTACAAG 
GCCAAAATTG AGGACCTGGA ACATGAAATC CAGAAACTTA TGGCTGAAAA GAATGATCTT 
GAGATCAAGG CTGAGGAAGC CTTGCAAGAT TCAGGGAAAA AAGACTTCAA GGACGAGATT 
CATGTTATGG CTGCATCACT CTCCAAAGAG ATGGAATTGT TGGACAATCA AATGAATAGA 
TCCAAGGATG CGGCTTCTGA AGCACTTGCA CTACGTGAGG AAGCTGACTA TTTAAGAACT 
TTGCTAGCTA AGAAGATTGA TGAACAAAAG GAGATATCTG ATAGATACAA CACACAAGTC 
ACCGAGATCA AATCCCTCAA AGCATTGATT GAGACACTGG ACCAGGAAAA GCAGGAGTTA 
CAATTTATAG TGGATATGCT CGGTAAAGAA TGTTCTGAGT CGAGGGCAAT TTCAGAGATT 
GAAGAATCAG AAAACCGAGC TCGCAAGCAA GCTGAATATT TGAGAAAATG TTTAGAAGAG 
CATAATCTTG AACTTCGAGT AAAAGCAGCA AATGAAGCGG AAACTGCATG CCAACAAAGG 
CTTTCAATTG CTGAAGCAGA ACTGGAAGAC TTAAGGGCCA AAGTGGATGC ATCGGAAAGA 
GATGTTATGA AACTCAAGGA GTCAATAAGA ATTAAAGAAG CTGAAGTAGA TGGCCATATT 
TCTGAAATTG AGACAATTGG TCAAGCATAT GAAGACATGC AAACCCAAAA TCAGCACCTT 
CTTCAACAGG TTGCTGATAG AGATGATTTT AACATAAAGC TAGTATCAGA TAGTGTAAAG 
ATGAAGCAAG CCTATGGTTC CCTTCTTGCT GAAAAGAATA TGCTACAGAA GCAACTTCAG 
CATGTCAACA GTTCACTGGA GTCATCTAAA CTAAAAATCA CCAGTGGTGA AGAGCAGATG 
AAGACCTATG TAGCACAAGC TATGAAATCT TCTTCAGAGA ATAGGCATCT TGCAATTAGC 
CTTGAAAGGA CTATGCTGGA GGTATCAGAT GCAGAGAAGG AGCTCAAGTG GCTTCGGTCC 
GCTACTGGAT CTGCTGAGAA AGAATATGAG ATAAATCAGA AAAAAATAGC TGAGCTAAAA 
ATGGAGCTAG AGCGTGAGAG AAATGAGAGG ATAAAGCTTG AGGAAGAATA CGAAGAGGTG 
AAAAACGAAG TCAGTGAGCT AACCTCAGAA ACTGAAGAGA CTACTATACA GAAGCTCCAA 
GATGAAATAA AAGAATGCAA GGCTATTCTC AAGTGTGGGG TGTGCTTCGA CCGGCCGAAA 
GAGGTCGTGA TCACCAAATG CTTCCACTTA TTCTGCTCGC CATGTATCCA GAGGAACCTT 
GAGATCCGCC ACCGCAAATG CCCAGGTTGT GGCACCCCCT TTGGACAGAG TGACGTTCGG 
GAGGTGAAGA TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC4349420 ( XM_026020810.1 ) cDNA ORF clone, Oryza sativa Japonica Group(Japanese rice) -> XP_025876595.1 Oryza sativa Japonica Group E3 ubiquitin-protein ligase BRE1-like 2 (LOC4349420), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OOa21059
Clone ID Related Accession (Same CDS sequence)	XM_015759100.1 , XM_026020810.1 , XM_015759100.2
Accession Version	XM_026020810.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2535bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-08-06
Organism	Oryza sativa Japonica Group(Japanese rice)
Product	E3 ubiquitin-protein ligase BRE1-like 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_029265.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Oryza sativa Japonica Group Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGCCG CAGCTCTTCA GTATGAGAAC CAGAAGCTGG TGCAGCAATT GGAGGCACAG 
AAGTCCAAAA TGCGTGCATT GGAAGGCAAG TTCAAGGAGC TGAGGGATGA GCAATGTTCT 
TACGACAATA CATTGATTTG TTTGAATAAA ATGTGGAATC AGCTGATTGA TGATTTAGTG 
CTGCTTGGGG TTCGAGCTGG TGGGGATTTA AACGGTTTGC AAGCGCTTGA TCATGAAGAG 
ATGTCAGAAG AATCTCTTGA GTCATGTCCT TCTGAGGAGA TATTTCTCTT CAGACTCTTG 
AATTCCAGGA ATTTCAGAAA TAATGATGAC AGTAGCCTGT CAAAATTAGT TGAGGAAGCT 
CTTGCCTTGC GCTATTCAAC AACAGTTACT CTGATGAAGT CCCTGCAAGA GGCTTTTGCT 
GTACAGCAGG CTAGAAGTGA ATCTTTATCA TTAGCTTTAA ATGGACAGAA CTCCAGTGAA 
GACGTTATTG TGGCCCTTGA AAACCATAAT GATTACTTGA AAGAAGTGGT TGACAATTTA 
CGTCAAGCTG TGTCTATCAT CAACAGGAAG CACGAAAAGT ATCTGGATGA GATTGAGGCT 
TTCAAAAACA ATCAATCAAG AGAACTACAT GAAGTAAAGT GTCTTTCAGG TGAGCTAGAG 
GAAAGCATGG CAGAGCTTGA GGAAAGCCGT CGAAAGTTGG CTGTTCTCCA GTTGCAAACG 
GGTGGAGGCT CACTGATGAA CACATCTGCT CCTAACGGTG TGAACGGTAG TGTTTCAACT 
GATAAATCTT CAGACAAGGG TATGGGCTGG CGAGATCTGA AAGATGCTGT TGAGGAGGCT 
AAGACTCTTG CTGCAAACCG CCTATTTGAA CTCCATGAGA CTCAAGAGGA TAATCTGATA 
CTATCCAAGC AGTTGGAAGA TATTCAGGAT CAATTAAAAG ATGAGAACTA TATTGTTACA 
TCAAAACCAT ATACGATTCT CAGTGACCAG TTACACCATC TGAATGCTGA GATAGAGCGA 
TACAGGGGGT TAGTTGAAGT GCTACAGAAT GAAAAGGACC AGCTCATGCA AAAGGAAGAA 
GAAATGCTTG CAAAGGCAGA ATCAGTGGAC GCTGTTCAAC AATCCATTAC CACTTACAAG 
GCCAAAATTG AGGACCTGGA ACATGAAATC CAGAAACTTA TGGCTGAAAA GAATGATCTT 
GAGATCAAGG CTGAGGAAGC CTTGCAAGAT TCAGGGAAAA AAGACTTCAA GGACGAGATT 
CATGTTATGG CTGCATCACT CTCCAAAGAG ATGGAATTGT TGGACAATCA AATGAATAGA 
TCCAAGGATG CGGCTTCTGA AGCACTTGCA CTACGTGAGG AAGCTGACTA TTTAAGAACT 
TTGCTAGCTA AGAAGATTGA TGAACAAAAG GAGATATCTG ATAGATACAA CACACAAGTC 
ACCGAGATCA AATCCCTCAA AGCATTGATT GAGACACTGG ACCAGGAAAA GCAGGAGTTA 
CAATTTATAG TGGATATGCT CGGTAAAGAA TGTTCTGAGT CGAGGGCAAT TTCAGAGATT 
GAAGAATCAG AAAACCGAGC TCGCAAGCAA GCTGAATATT TGAGAAAATG TTTAGAAGAG 
CATAATCTTG AACTTCGAGT AAAAGCAGCA AATGAAGCGG AAACTGCATG CCAACAAAGG 
CTTTCAATTG CTGAAGCAGA ACTGGAAGAC TTAAGGGCCA AAGTGGATGC ATCGGAAAGA 
GATGTTATGA AACTCAAGGA GTCAATAAGA ATTAAAGAAG CTGAAGTAGA TGGCCATATT 
TCTGAAATTG AGACAATTGG TCAAGCATAT GAAGACATGC AAACCCAAAA TCAGCACCTT 
CTTCAACAGG TTGCTGATAG AGATGATTTT AACATAAAGC TAGTATCAGA TAGTGTAAAG 
ATGAAGCAAG CCTATGGTTC CCTTCTTGCT GAAAAGAATA TGCTACAGAA GCAACTTCAG 
CATGTCAACA GTTCACTGGA GTCATCTAAA CTAAAAATCA CCAGTGGTGA AGAGCAGATG 
AAGACCTATG TAGCACAAGC TATGAAATCT TCTTCAGAGA ATAGGCATCT TGCAATTAGC 
CTTGAAAGGA CTATGCTGGA GGTATCAGAT GCAGAGAAGG AGCTCAAGTG GCTTCGGTCC 
GCTACTGGAT CTGCTGAGAA AGAATATGAG ATAAATCAGA AAAAAATAGC TGAGCTAAAA 
ATGGAGCTAG AGCGTGAGAG AAATGAGAGG ATAAAGCTTG AGGAAGAATA CGAAGAGGTG 
AAAAACGAAG TCAGTGAGCT AACCTCAGAA ACTGAAGAGA CTACTATACA GAAGCTCCAA 
GATGAAATAA AAGAATGCAA GGCTATTCTC AAGTGTGGGG TGTGCTTCGA CCGGCCGAAA 
GAGGTCGTGA TCACCAAATG CTTCCACTTA TTCTGCTCGC CATGTATCCA GAGGAACCTT 
GAGATCCGCC ACCGCAAATG CCCAGGTTGT GGCACCCCCT TTGGACAGAG TGACGTTCGG 
GAGGTGAAGA TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025876595.1
CDS	150..2684
Translation	MDAAALQYENQKLVQQLEAQKSKMRALEGKFKELRDEQCSYDNTLICLNKMWNQLIDDLVLLGVRAGGDLNGLQALDHEEMSEESLESCPSEEIFLFRLLNSRNFRNNDDSSLSKLVEEALALRYSTTVTLMKSLQEAFAVQQARSESLSLALNGQNSSEDVIVALENHNDYLKEVVDNLRQAVSIINRKHEKYLDEIEAFKNNQSRELHEVKCLSGELEESMAELEESRRKLAVLQLQTGGGSLMNTSAPNGVNGSVSTDKSSDKGMGWRDLKDAVEEAKTLAANRLFELHETQEDNLILSKQLEDIQDQLKDENYIVTSKPYTILSDQLHHLNAEIERYRGLVEVLQNEKDQLMQKEEEMLAKAESVDAVQQSITTYKAKIEDLEHEIQKLMAEKNDLEIKAEEALQDSGKKDFKDEIHVMAASLSKEMELLDNQMNRSKDAASEALALREEADYLRTLLAKKIDEQKEISDRYNTQVTEIKSLKALIETLDQEKQELQFIVDMLGKECSESRAISEIEESENRARKQAEYLRKCLEEHNLELRVKAANEAETACQQRLSIAEAELEDLRAKVDASERDVMKLKESIRIKEAEVDGHISEIETIGQAYEDMQTQNQHLLQQVADRDDFNIKLVSDSVKMKQAYGSLLAEKNMLQKQLQHVNSSLESSKLKITSGEEQMKTYVAQAMKSSSENRHLAISLERTMLEVSDAEKELKWLRSATGSAEKEYEINQKKIAELKMELERERNERIKLEEEYEEVKNEVSELTSETEETTIQKLQDEIKECKAILKCGVCFDRPKEVVITKCFHLFCSPCIQRNLEIRHRKCPGCGTPFGQSDVREVKI

Target ORF information:

RefSeq Version	XM_026020810.1
Organism	Oryza sativa Japonica Group(Japanese rice)
Definition	Oryza sativa Japonica Group E3 ubiquitin-protein ligase BRE1-like 2 (LOC4349420), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_026020810.1

ORF Insert Sequence:

ATGGATGCCG CAGCTCTTCA GTATGAGAAC CAGAAGCTGG TGCAGCAATT GGAGGCACAG 
AAGTCCAAAA TGCGTGCATT GGAAGGCAAG TTCAAGGAGC TGAGGGATGA GCAATGTTCT 
TACGACAATA CATTGATTTG TTTGAATAAA ATGTGGAATC AGCTGATTGA TGATTTAGTG 
CTGCTTGGGG TTCGAGCTGG TGGGGATTTA AACGGTTTGC AAGCGCTTGA TCATGAAGAG 
ATGTCAGAAG AATCTCTTGA GTCATGTCCT TCTGAGGAGA TATTTCTCTT CAGACTCTTG 
AATTCCAGGA ATTTCAGAAA TAATGATGAC AGTAGCCTGT CAAAATTAGT TGAGGAAGCT 
CTTGCCTTGC GCTATTCAAC AACAGTTACT CTGATGAAGT CCCTGCAAGA GGCTTTTGCT 
GTACAGCAGG CTAGAAGTGA ATCTTTATCA TTAGCTTTAA ATGGACAGAA CTCCAGTGAA 
GACGTTATTG TGGCCCTTGA AAACCATAAT GATTACTTGA AAGAAGTGGT TGACAATTTA 
CGTCAAGCTG TGTCTATCAT CAACAGGAAG CACGAAAAGT ATCTGGATGA GATTGAGGCT 
TTCAAAAACA ATCAATCAAG AGAACTACAT GAAGTAAAGT GTCTTTCAGG TGAGCTAGAG 
GAAAGCATGG CAGAGCTTGA GGAAAGCCGT CGAAAGTTGG CTGTTCTCCA GTTGCAAACG 
GGTGGAGGCT CACTGATGAA CACATCTGCT CCTAACGGTG TGAACGGTAG TGTTTCAACT 
GATAAATCTT CAGACAAGGG TATGGGCTGG CGAGATCTGA AAGATGCTGT TGAGGAGGCT 
AAGACTCTTG CTGCAAACCG CCTATTTGAA CTCCATGAGA CTCAAGAGGA TAATCTGATA 
CTATCCAAGC AGTTGGAAGA TATTCAGGAT CAATTAAAAG ATGAGAACTA TATTGTTACA 
TCAAAACCAT ATACGATTCT CAGTGACCAG TTACACCATC TGAATGCTGA GATAGAGCGA 
TACAGGGGGT TAGTTGAAGT GCTACAGAAT GAAAAGGACC AGCTCATGCA AAAGGAAGAA 
GAAATGCTTG CAAAGGCAGA ATCAGTGGAC GCTGTTCAAC AATCCATTAC CACTTACAAG 
GCCAAAATTG AGGACCTGGA ACATGAAATC CAGAAACTTA TGGCTGAAAA GAATGATCTT 
GAGATCAAGG CTGAGGAAGC CTTGCAAGAT TCAGGGAAAA AAGACTTCAA GGACGAGATT 
CATGTTATGG CTGCATCACT CTCCAAAGAG ATGGAATTGT TGGACAATCA AATGAATAGA 
TCCAAGGATG CGGCTTCTGA AGCACTTGCA CTACGTGAGG AAGCTGACTA TTTAAGAACT 
TTGCTAGCTA AGAAGATTGA TGAACAAAAG GAGATATCTG ATAGATACAA CACACAAGTC 
ACCGAGATCA AATCCCTCAA AGCATTGATT GAGACACTGG ACCAGGAAAA GCAGGAGTTA 
CAATTTATAG TGGATATGCT CGGTAAAGAA TGTTCTGAGT CGAGGGCAAT TTCAGAGATT 
GAAGAATCAG AAAACCGAGC TCGCAAGCAA GCTGAATATT TGAGAAAATG TTTAGAAGAG 
CATAATCTTG AACTTCGAGT AAAAGCAGCA AATGAAGCGG AAACTGCATG CCAACAAAGG 
CTTTCAATTG CTGAAGCAGA ACTGGAAGAC TTAAGGGCCA AAGTGGATGC ATCGGAAAGA 
GATGTTATGA AACTCAAGGA GTCAATAAGA ATTAAAGAAG CTGAAGTAGA TGGCCATATT 
TCTGAAATTG AGACAATTGG TCAAGCATAT GAAGACATGC AAACCCAAAA TCAGCACCTT 
CTTCAACAGG TTGCTGATAG AGATGATTTT AACATAAAGC TAGTATCAGA TAGTGTAAAG 
ATGAAGCAAG CCTATGGTTC CCTTCTTGCT GAAAAGAATA TGCTACAGAA GCAACTTCAG 
CATGTCAACA GTTCACTGGA GTCATCTAAA CTAAAAATCA CCAGTGGTGA AGAGCAGATG 
AAGACCTATG TAGCACAAGC TATGAAATCT TCTTCAGAGA ATAGGCATCT TGCAATTAGC 
CTTGAAAGGA CTATGCTGGA GGTATCAGAT GCAGAGAAGG AGCTCAAGTG GCTTCGGTCC 
GCTACTGGAT CTGCTGAGAA AGAATATGAG ATAAATCAGA AAAAAATAGC TGAGCTAAAA 
ATGGAGCTAG AGCGTGAGAG AAATGAGAGG ATAAAGCTTG AGGAAGAATA CGAAGAGGTG 
AAAAACGAAG TCAGTGAGCT AACCTCAGAA ACTGAAGAGA CTACTATACA GAAGCTCCAA 
GATGAAATAA AAGAATGCAA GGCTATTCTC AAGTGTGGGG TGTGCTTCGA CCGGCCGAAA 
GAGGTCGTGA TCACCAAATG CTTCCACTTA TTCTGCTCGC CATGTATCCA GAGGAACCTT 
GAGATCCGCC ACCGCAAATG CCCAGGTTGT GGCACCCCCT TTGGACAGAG TGACGTTCGG 
GAGGTGAAGA TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC4349420 ( XM_015759100.2 ) cDNA ORF clone, Oryza sativa Japonica Group(Japanese rice) -> XP_015614586.1 Oryza sativa Japonica Group E3 ubiquitin-protein ligase BRE1-like 2 (LOC4349420), transcript variant X1, mRNA.

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CloneID	OOa21059
Clone ID Related Accession (Same CDS sequence)	XM_015759100.1 , XM_026020810.1 , XM_015759100.2
Accession Version	XM_015759100.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2535bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-08-06
Organism	Oryza sativa Japonica Group(Japanese rice)
Product	E3 ubiquitin-protein ligase BRE1-like 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_029265.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 7, 2018 this sequence version replaced XM_015759100.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Oryza sativa Japonica Group Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGCCG CAGCTCTTCA GTATGAGAAC CAGAAGCTGG TGCAGCAATT GGAGGCACAG 
AAGTCCAAAA TGCGTGCATT GGAAGGCAAG TTCAAGGAGC TGAGGGATGA GCAATGTTCT 
TACGACAATA CATTGATTTG TTTGAATAAA ATGTGGAATC AGCTGATTGA TGATTTAGTG 
CTGCTTGGGG TTCGAGCTGG TGGGGATTTA AACGGTTTGC AAGCGCTTGA TCATGAAGAG 
ATGTCAGAAG AATCTCTTGA GTCATGTCCT TCTGAGGAGA TATTTCTCTT CAGACTCTTG 
AATTCCAGGA ATTTCAGAAA TAATGATGAC AGTAGCCTGT CAAAATTAGT TGAGGAAGCT 
CTTGCCTTGC GCTATTCAAC AACAGTTACT CTGATGAAGT CCCTGCAAGA GGCTTTTGCT 
GTACAGCAGG CTAGAAGTGA ATCTTTATCA TTAGCTTTAA ATGGACAGAA CTCCAGTGAA 
GACGTTATTG TGGCCCTTGA AAACCATAAT GATTACTTGA AAGAAGTGGT TGACAATTTA 
CGTCAAGCTG TGTCTATCAT CAACAGGAAG CACGAAAAGT ATCTGGATGA GATTGAGGCT 
TTCAAAAACA ATCAATCAAG AGAACTACAT GAAGTAAAGT GTCTTTCAGG TGAGCTAGAG 
GAAAGCATGG CAGAGCTTGA GGAAAGCCGT CGAAAGTTGG CTGTTCTCCA GTTGCAAACG 
GGTGGAGGCT CACTGATGAA CACATCTGCT CCTAACGGTG TGAACGGTAG TGTTTCAACT 
GATAAATCTT CAGACAAGGG TATGGGCTGG CGAGATCTGA AAGATGCTGT TGAGGAGGCT 
AAGACTCTTG CTGCAAACCG CCTATTTGAA CTCCATGAGA CTCAAGAGGA TAATCTGATA 
CTATCCAAGC AGTTGGAAGA TATTCAGGAT CAATTAAAAG ATGAGAACTA TATTGTTACA 
TCAAAACCAT ATACGATTCT CAGTGACCAG TTACACCATC TGAATGCTGA GATAGAGCGA 
TACAGGGGGT TAGTTGAAGT GCTACAGAAT GAAAAGGACC AGCTCATGCA AAAGGAAGAA 
GAAATGCTTG CAAAGGCAGA ATCAGTGGAC GCTGTTCAAC AATCCATTAC CACTTACAAG 
GCCAAAATTG AGGACCTGGA ACATGAAATC CAGAAACTTA TGGCTGAAAA GAATGATCTT 
GAGATCAAGG CTGAGGAAGC CTTGCAAGAT TCAGGGAAAA AAGACTTCAA GGACGAGATT 
CATGTTATGG CTGCATCACT CTCCAAAGAG ATGGAATTGT TGGACAATCA AATGAATAGA 
TCCAAGGATG CGGCTTCTGA AGCACTTGCA CTACGTGAGG AAGCTGACTA TTTAAGAACT 
TTGCTAGCTA AGAAGATTGA TGAACAAAAG GAGATATCTG ATAGATACAA CACACAAGTC 
ACCGAGATCA AATCCCTCAA AGCATTGATT GAGACACTGG ACCAGGAAAA GCAGGAGTTA 
CAATTTATAG TGGATATGCT CGGTAAAGAA TGTTCTGAGT CGAGGGCAAT TTCAGAGATT 
GAAGAATCAG AAAACCGAGC TCGCAAGCAA GCTGAATATT TGAGAAAATG TTTAGAAGAG 
CATAATCTTG AACTTCGAGT AAAAGCAGCA AATGAAGCGG AAACTGCATG CCAACAAAGG 
CTTTCAATTG CTGAAGCAGA ACTGGAAGAC TTAAGGGCCA AAGTGGATGC ATCGGAAAGA 
GATGTTATGA AACTCAAGGA GTCAATAAGA ATTAAAGAAG CTGAAGTAGA TGGCCATATT 
TCTGAAATTG AGACAATTGG TCAAGCATAT GAAGACATGC AAACCCAAAA TCAGCACCTT 
CTTCAACAGG TTGCTGATAG AGATGATTTT AACATAAAGC TAGTATCAGA TAGTGTAAAG 
ATGAAGCAAG CCTATGGTTC CCTTCTTGCT GAAAAGAATA TGCTACAGAA GCAACTTCAG 
CATGTCAACA GTTCACTGGA GTCATCTAAA CTAAAAATCA CCAGTGGTGA AGAGCAGATG 
AAGACCTATG TAGCACAAGC TATGAAATCT TCTTCAGAGA ATAGGCATCT TGCAATTAGC 
CTTGAAAGGA CTATGCTGGA GGTATCAGAT GCAGAGAAGG AGCTCAAGTG GCTTCGGTCC 
GCTACTGGAT CTGCTGAGAA AGAATATGAG ATAAATCAGA AAAAAATAGC TGAGCTAAAA 
ATGGAGCTAG AGCGTGAGAG AAATGAGAGG ATAAAGCTTG AGGAAGAATA CGAAGAGGTG 
AAAAACGAAG TCAGTGAGCT AACCTCAGAA ACTGAAGAGA CTACTATACA GAAGCTCCAA 
GATGAAATAA AAGAATGCAA GGCTATTCTC AAGTGTGGGG TGTGCTTCGA CCGGCCGAAA 
GAGGTCGTGA TCACCAAATG CTTCCACTTA TTCTGCTCGC CATGTATCCA GAGGAACCTT 
GAGATCCGCC ACCGCAAATG CCCAGGTTGT GGCACCCCCT TTGGACAGAG TGACGTTCGG 
GAGGTGAAGA TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015614586.1
CDS	156..2690
Translation	MDAAALQYENQKLVQQLEAQKSKMRALEGKFKELRDEQCSYDNTLICLNKMWNQLIDDLVLLGVRAGGDLNGLQALDHEEMSEESLESCPSEEIFLFRLLNSRNFRNNDDSSLSKLVEEALALRYSTTVTLMKSLQEAFAVQQARSESLSLALNGQNSSEDVIVALENHNDYLKEVVDNLRQAVSIINRKHEKYLDEIEAFKNNQSRELHEVKCLSGELEESMAELEESRRKLAVLQLQTGGGSLMNTSAPNGVNGSVSTDKSSDKGMGWRDLKDAVEEAKTLAANRLFELHETQEDNLILSKQLEDIQDQLKDENYIVTSKPYTILSDQLHHLNAEIERYRGLVEVLQNEKDQLMQKEEEMLAKAESVDAVQQSITTYKAKIEDLEHEIQKLMAEKNDLEIKAEEALQDSGKKDFKDEIHVMAASLSKEMELLDNQMNRSKDAASEALALREEADYLRTLLAKKIDEQKEISDRYNTQVTEIKSLKALIETLDQEKQELQFIVDMLGKECSESRAISEIEESENRARKQAEYLRKCLEEHNLELRVKAANEAETACQQRLSIAEAELEDLRAKVDASERDVMKLKESIRIKEAEVDGHISEIETIGQAYEDMQTQNQHLLQQVADRDDFNIKLVSDSVKMKQAYGSLLAEKNMLQKQLQHVNSSLESSKLKITSGEEQMKTYVAQAMKSSSENRHLAISLERTMLEVSDAEKELKWLRSATGSAEKEYEINQKKIAELKMELERERNERIKLEEEYEEVKNEVSELTSETEETTIQKLQDEIKECKAILKCGVCFDRPKEVVITKCFHLFCSPCIQRNLEIRHRKCPGCGTPFGQSDVREVKI

Target ORF information:

RefSeq Version	XM_015759100.2
Organism	Oryza sativa Japonica Group(Japanese rice)
Definition	Oryza sativa Japonica Group E3 ubiquitin-protein ligase BRE1-like 2 (LOC4349420), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015759100.2

ORF Insert Sequence:

ATGGATGCCG CAGCTCTTCA GTATGAGAAC CAGAAGCTGG TGCAGCAATT GGAGGCACAG 
AAGTCCAAAA TGCGTGCATT GGAAGGCAAG TTCAAGGAGC TGAGGGATGA GCAATGTTCT 
TACGACAATA CATTGATTTG TTTGAATAAA ATGTGGAATC AGCTGATTGA TGATTTAGTG 
CTGCTTGGGG TTCGAGCTGG TGGGGATTTA AACGGTTTGC AAGCGCTTGA TCATGAAGAG 
ATGTCAGAAG AATCTCTTGA GTCATGTCCT TCTGAGGAGA TATTTCTCTT CAGACTCTTG 
AATTCCAGGA ATTTCAGAAA TAATGATGAC AGTAGCCTGT CAAAATTAGT TGAGGAAGCT 
CTTGCCTTGC GCTATTCAAC AACAGTTACT CTGATGAAGT CCCTGCAAGA GGCTTTTGCT 
GTACAGCAGG CTAGAAGTGA ATCTTTATCA TTAGCTTTAA ATGGACAGAA CTCCAGTGAA 
GACGTTATTG TGGCCCTTGA AAACCATAAT GATTACTTGA AAGAAGTGGT TGACAATTTA 
CGTCAAGCTG TGTCTATCAT CAACAGGAAG CACGAAAAGT ATCTGGATGA GATTGAGGCT 
TTCAAAAACA ATCAATCAAG AGAACTACAT GAAGTAAAGT GTCTTTCAGG TGAGCTAGAG 
GAAAGCATGG CAGAGCTTGA GGAAAGCCGT CGAAAGTTGG CTGTTCTCCA GTTGCAAACG 
GGTGGAGGCT CACTGATGAA CACATCTGCT CCTAACGGTG TGAACGGTAG TGTTTCAACT 
GATAAATCTT CAGACAAGGG TATGGGCTGG CGAGATCTGA AAGATGCTGT TGAGGAGGCT 
AAGACTCTTG CTGCAAACCG CCTATTTGAA CTCCATGAGA CTCAAGAGGA TAATCTGATA 
CTATCCAAGC AGTTGGAAGA TATTCAGGAT CAATTAAAAG ATGAGAACTA TATTGTTACA 
TCAAAACCAT ATACGATTCT CAGTGACCAG TTACACCATC TGAATGCTGA GATAGAGCGA 
TACAGGGGGT TAGTTGAAGT GCTACAGAAT GAAAAGGACC AGCTCATGCA AAAGGAAGAA 
GAAATGCTTG CAAAGGCAGA ATCAGTGGAC GCTGTTCAAC AATCCATTAC CACTTACAAG 
GCCAAAATTG AGGACCTGGA ACATGAAATC CAGAAACTTA TGGCTGAAAA GAATGATCTT 
GAGATCAAGG CTGAGGAAGC CTTGCAAGAT TCAGGGAAAA AAGACTTCAA GGACGAGATT 
CATGTTATGG CTGCATCACT CTCCAAAGAG ATGGAATTGT TGGACAATCA AATGAATAGA 
TCCAAGGATG CGGCTTCTGA AGCACTTGCA CTACGTGAGG AAGCTGACTA TTTAAGAACT 
TTGCTAGCTA AGAAGATTGA TGAACAAAAG GAGATATCTG ATAGATACAA CACACAAGTC 
ACCGAGATCA AATCCCTCAA AGCATTGATT GAGACACTGG ACCAGGAAAA GCAGGAGTTA 
CAATTTATAG TGGATATGCT CGGTAAAGAA TGTTCTGAGT CGAGGGCAAT TTCAGAGATT 
GAAGAATCAG AAAACCGAGC TCGCAAGCAA GCTGAATATT TGAGAAAATG TTTAGAAGAG 
CATAATCTTG AACTTCGAGT AAAAGCAGCA AATGAAGCGG AAACTGCATG CCAACAAAGG 
CTTTCAATTG CTGAAGCAGA ACTGGAAGAC TTAAGGGCCA AAGTGGATGC ATCGGAAAGA 
GATGTTATGA AACTCAAGGA GTCAATAAGA ATTAAAGAAG CTGAAGTAGA TGGCCATATT 
TCTGAAATTG AGACAATTGG TCAAGCATAT GAAGACATGC AAACCCAAAA TCAGCACCTT 
CTTCAACAGG TTGCTGATAG AGATGATTTT AACATAAAGC TAGTATCAGA TAGTGTAAAG 
ATGAAGCAAG CCTATGGTTC CCTTCTTGCT GAAAAGAATA TGCTACAGAA GCAACTTCAG 
CATGTCAACA GTTCACTGGA GTCATCTAAA CTAAAAATCA CCAGTGGTGA AGAGCAGATG 
AAGACCTATG TAGCACAAGC TATGAAATCT TCTTCAGAGA ATAGGCATCT TGCAATTAGC 
CTTGAAAGGA CTATGCTGGA GGTATCAGAT GCAGAGAAGG AGCTCAAGTG GCTTCGGTCC 
GCTACTGGAT CTGCTGAGAA AGAATATGAG ATAAATCAGA AAAAAATAGC TGAGCTAAAA 
ATGGAGCTAG AGCGTGAGAG AAATGAGAGG ATAAAGCTTG AGGAAGAATA CGAAGAGGTG 
AAAAACGAAG TCAGTGAGCT AACCTCAGAA ACTGAAGAGA CTACTATACA GAAGCTCCAA 
GATGAAATAA AAGAATGCAA GGCTATTCTC AAGTGTGGGG TGTGCTTCGA CCGGCCGAAA 
GAGGTCGTGA TCACCAAATG CTTCCACTTA TTCTGCTCGC CATGTATCCA GAGGAACCTT 
GAGATCCGCC ACCGCAAATG CCCAGGTTGT GGCACCCCCT TTGGACAGAG TGACGTTCGG 
GAGGTGAAGA TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC4349420 ( XM_026020811.1 ) cDNA ORF clone, Oryza sativa Japonica Group(Japanese rice) -> XP_025876596.1 Oryza sativa Japonica Group E3 ubiquitin-protein ligase BRE1-like 2 (LOC4349420), transcript variant X3, mRNA.

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CloneID	OOa44694
Clone ID Related Accession (Same CDS sequence)	XM_026020811.1
Accession Version	XM_026020811.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1869bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-08-06
Organism	Oryza sativa Japonica Group(Japanese rice)
Product	E3 ubiquitin-protein ligase BRE1-like 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_029265.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Oryza sativa Japonica Group Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGAGC TTGAGGAAAG CCGTCGAAAG TTGGCTGTTC TCCAGTTGCA AACGGGTGGA 
GGCTCACTGA TGAACACATC TGCTCCTAAC GGTGTGAACG GTAGTGTTTC AACTGATAAA 
TCTTCAGACA AGGGTATGGG CTGGCGAGAT CTGAAAGATG CTGTTGAGGA GGCTAAGACT 
CTTGCTGCAA ACCGCCTATT TGAACTCCAT GAGACTCAAG AGGATAATCT GATACTATCC 
AAGCAGTTGG AAGATATTCA GGATCAATTA AAAGATGAGA ACTATATTGT TACATCAAAA 
CCATATACGA TTCTCAGTGA CCAGTTACAC CATCTGAATG CTGAGATAGA GCGATACAGG 
GGGTTAGTTG AAGTGCTACA GAATGAAAAG GACCAGCTCA TGCAAAAGGA AGAAGAAATG 
CTTGCAAAGG CAGAATCAGT GGACGCTGTT CAACAATCCA TTACCACTTA CAAGGCCAAA 
ATTGAGGACC TGGAACATGA AATCCAGAAA CTTATGGCTG AAAAGAATGA TCTTGAGATC 
AAGGCTGAGG AAGCCTTGCA AGATTCAGGG AAAAAAGACT TCAAGGACGA GATTCATGTT 
ATGGCTGCAT CACTCTCCAA AGAGATGGAA TTGTTGGACA ATCAAATGAA TAGATCCAAG 
GATGCGGCTT CTGAAGCACT TGCACTACGT GAGGAAGCTG ACTATTTAAG AACTTTGCTA 
GCTAAGAAGA TTGATGAACA AAAGGAGATA TCTGATAGAT ACAACACACA AGTCACCGAG 
ATCAAATCCC TCAAAGCATT GATTGAGACA CTGGACCAGG AAAAGCAGGA GTTACAATTT 
ATAGTGGATA TGCTCGGTAA AGAATGTTCT GAGTCGAGGG CAATTTCAGA GATTGAAGAA 
TCAGAAAACC GAGCTCGCAA GCAAGCTGAA TATTTGAGAA AATGTTTAGA AGAGCATAAT 
CTTGAACTTC GAGTAAAAGC AGCAAATGAA GCGGAAACTG CATGCCAACA AAGGCTTTCA 
ATTGCTGAAG CAGAACTGGA AGACTTAAGG GCCAAAGTGG ATGCATCGGA AAGAGATGTT 
ATGAAACTCA AGGAGTCAAT AAGAATTAAA GAAGCTGAAG TAGATGGCCA TATTTCTGAA 
ATTGAGACAA TTGGTCAAGC ATATGAAGAC ATGCAAACCC AAAATCAGCA CCTTCTTCAA 
CAGGTTGCTG ATAGAGATGA TTTTAACATA AAGCTAGTAT CAGATAGTGT AAAGATGAAG 
CAAGCCTATG GTTCCCTTCT TGCTGAAAAG AATATGCTAC AGAAGCAACT TCAGCATGTC 
AACAGTTCAC TGGAGTCATC TAAACTAAAA ATCACCAGTG GTGAAGAGCA GATGAAGACC 
TATGTAGCAC AAGCTATGAA ATCTTCTTCA GAGAATAGGC ATCTTGCAAT TAGCCTTGAA 
AGGACTATGC TGGAGGTATC AGATGCAGAG AAGGAGCTCA AGTGGCTTCG GTCCGCTACT 
GGATCTGCTG AGAAAGAATA TGAGATAAAT CAGAAAAAAA TAGCTGAGCT AAAAATGGAG 
CTAGAGCGTG AGAGAAATGA GAGGATAAAG CTTGAGGAAG AATACGAAGA GGTGAAAAAC 
GAAGTCAGTG AGCTAACCTC AGAAACTGAA GAGACTACTA TACAGAAGCT CCAAGATGAA 
ATAAAAGAAT GCAAGGCTAT TCTCAAGTGT GGGGTGTGCT TCGACCGGCC GAAAGAGGTC 
GTGATCACCA AATGCTTCCA CTTATTCTGC TCGCCATGTA TCCAGAGGAA CCTTGAGATC 
CGCCACCGCA AATGCCCAGG TTGTGGCACC CCCTTTGGAC AGAGTGACGT TCGGGAGGTG 
AAGATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025876596.1
CDS	244..2112
Translation	MAELEESRRKLAVLQLQTGGGSLMNTSAPNGVNGSVSTDKSSDKGMGWRDLKDAVEEAKTLAANRLFELHETQEDNLILSKQLEDIQDQLKDENYIVTSKPYTILSDQLHHLNAEIERYRGLVEVLQNEKDQLMQKEEEMLAKAESVDAVQQSITTYKAKIEDLEHEIQKLMAEKNDLEIKAEEALQDSGKKDFKDEIHVMAASLSKEMELLDNQMNRSKDAASEALALREEADYLRTLLAKKIDEQKEISDRYNTQVTEIKSLKALIETLDQEKQELQFIVDMLGKECSESRAISEIEESENRARKQAEYLRKCLEEHNLELRVKAANEAETACQQRLSIAEAELEDLRAKVDASERDVMKLKESIRIKEAEVDGHISEIETIGQAYEDMQTQNQHLLQQVADRDDFNIKLVSDSVKMKQAYGSLLAEKNMLQKQLQHVNSSLESSKLKITSGEEQMKTYVAQAMKSSSENRHLAISLERTMLEVSDAEKELKWLRSATGSAEKEYEINQKKIAELKMELERERNERIKLEEEYEEVKNEVSELTSETEETTIQKLQDEIKECKAILKCGVCFDRPKEVVITKCFHLFCSPCIQRNLEIRHRKCPGCGTPFGQSDVREVKI

Target ORF information:

RefSeq Version	XM_026020811.1
Organism	Oryza sativa Japonica Group(Japanese rice)
Definition	Oryza sativa Japonica Group E3 ubiquitin-protein ligase BRE1-like 2 (LOC4349420), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_026020811.1

ORF Insert Sequence:

ATGGCAGAGC TTGAGGAAAG CCGTCGAAAG TTGGCTGTTC TCCAGTTGCA AACGGGTGGA 
GGCTCACTGA TGAACACATC TGCTCCTAAC GGTGTGAACG GTAGTGTTTC AACTGATAAA 
TCTTCAGACA AGGGTATGGG CTGGCGAGAT CTGAAAGATG CTGTTGAGGA GGCTAAGACT 
CTTGCTGCAA ACCGCCTATT TGAACTCCAT GAGACTCAAG AGGATAATCT GATACTATCC 
AAGCAGTTGG AAGATATTCA GGATCAATTA AAAGATGAGA ACTATATTGT TACATCAAAA 
CCATATACGA TTCTCAGTGA CCAGTTACAC CATCTGAATG CTGAGATAGA GCGATACAGG 
GGGTTAGTTG AAGTGCTACA GAATGAAAAG GACCAGCTCA TGCAAAAGGA AGAAGAAATG 
CTTGCAAAGG CAGAATCAGT GGACGCTGTT CAACAATCCA TTACCACTTA CAAGGCCAAA 
ATTGAGGACC TGGAACATGA AATCCAGAAA CTTATGGCTG AAAAGAATGA TCTTGAGATC 
AAGGCTGAGG AAGCCTTGCA AGATTCAGGG AAAAAAGACT TCAAGGACGA GATTCATGTT 
ATGGCTGCAT CACTCTCCAA AGAGATGGAA TTGTTGGACA ATCAAATGAA TAGATCCAAG 
GATGCGGCTT CTGAAGCACT TGCACTACGT GAGGAAGCTG ACTATTTAAG AACTTTGCTA 
GCTAAGAAGA TTGATGAACA AAAGGAGATA TCTGATAGAT ACAACACACA AGTCACCGAG 
ATCAAATCCC TCAAAGCATT GATTGAGACA CTGGACCAGG AAAAGCAGGA GTTACAATTT 
ATAGTGGATA TGCTCGGTAA AGAATGTTCT GAGTCGAGGG CAATTTCAGA GATTGAAGAA 
TCAGAAAACC GAGCTCGCAA GCAAGCTGAA TATTTGAGAA AATGTTTAGA AGAGCATAAT 
CTTGAACTTC GAGTAAAAGC AGCAAATGAA GCGGAAACTG CATGCCAACA AAGGCTTTCA 
ATTGCTGAAG CAGAACTGGA AGACTTAAGG GCCAAAGTGG ATGCATCGGA AAGAGATGTT 
ATGAAACTCA AGGAGTCAAT AAGAATTAAA GAAGCTGAAG TAGATGGCCA TATTTCTGAA 
ATTGAGACAA TTGGTCAAGC ATATGAAGAC ATGCAAACCC AAAATCAGCA CCTTCTTCAA 
CAGGTTGCTG ATAGAGATGA TTTTAACATA AAGCTAGTAT CAGATAGTGT AAAGATGAAG 
CAAGCCTATG GTTCCCTTCT TGCTGAAAAG AATATGCTAC AGAAGCAACT TCAGCATGTC 
AACAGTTCAC TGGAGTCATC TAAACTAAAA ATCACCAGTG GTGAAGAGCA GATGAAGACC 
TATGTAGCAC AAGCTATGAA ATCTTCTTCA GAGAATAGGC ATCTTGCAAT TAGCCTTGAA 
AGGACTATGC TGGAGGTATC AGATGCAGAG AAGGAGCTCA AGTGGCTTCG GTCCGCTACT 
GGATCTGCTG AGAAAGAATA TGAGATAAAT CAGAAAAAAA TAGCTGAGCT AAAAATGGAG 
CTAGAGCGTG AGAGAAATGA GAGGATAAAG CTTGAGGAAG AATACGAAGA GGTGAAAAAC 
GAAGTCAGTG AGCTAACCTC AGAAACTGAA GAGACTACTA TACAGAAGCT CCAAGATGAA 
ATAAAAGAAT GCAAGGCTAT TCTCAAGTGT GGGGTGTGCT TCGACCGGCC GAAAGAGGTC 
GTGATCACCA AATGCTTCCA CTTATTCTGC TCGCCATGTA TCCAGAGGAA CCTTGAGATC 
CGCCACCGCA AATGCCCAGG TTGTGGCACC CCCTTTGGAC AGAGTGACGT TCGGGAGGTG 
AAGATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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LOC4349420 cDNA ORF clone, Oryza sativa Japonica Group(Japanese rice)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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FAX

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