LOC4332523 cDNA ORF clone, Oryza sativa Japonica Group(Japanese rice)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC4332523
Entrez Gene ID	4332523
Full Name	6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase
Gene Type	protein-coding
Organism	Oryza sativa Japonica Group(Japanese rice)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015774919.1	XP_015630405.1	6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase isoform X2
XM_015774920.2	XP_015630406.1	6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase isoform X3
XM_015774918.1	XP_015630404.1	6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase isoform X1
XM_015774920.1	XP_015630406.1	6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase isoform X3
XM_015774918.2	XP_015630404.1	6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase isoform X1
XM_015774919.2	XP_015630405.1	6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase isoform X2

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Pfkfb2	NP_032851.2
Magnaporthe oryzae (rice blast fungus)	MGG_06524	XP_003717027.1
Oryza sativa (rice)	Os03g0294200	NP_001049815.1
Homo sapiens (human)	PFKFB2	NP_006203.2
Danio rerio (zebrafish)	pfkfb2a	NP_957302.1
Kluyveromyces lactis	KLLA0B07513g	XP_451863.1
Pan troglodytes (chimpanzee)	PFKFB2	XP_001165997.2
	PFKFB2	XP_001082732.1
Schizosaccharomyces pombe (fission yeast)	SPAPB17E12.14c	NP_001018229.2
Neurospora crassa	NCU09347	XP_958926.1
Gallus gallus (chicken)	PFKFB2	XP_004935023.1
Eremothecium gossypii	AGOS_AEL106W	NP_984755.2
Canis lupus familiaris (dog)	PFKFB2	XP_005622364.1
Bos taurus (cattle)	PFKFB2	NP_777237.3
Rattus norvegicus (Norway rat)	Pfkfb2	NP_001029136.1
Danio rerio (zebrafish)	LOC564852	XP_001919792.3
Saccharomyces cerevisiae (baker's yeast)	FBP26	NP_012380.1
Xenopus tropicalis (tropical clawed frog)	pfkfb2	NP_001123395.1

Gene Ontology

The following LOC4332523 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC4332523 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OOa07115	XM_015774920.2 Latest version!	Oryza sativa Japonica Group 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase (LOC4332523), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OOa30063	XM_015774918.2 Latest version!	Oryza sativa Japonica Group 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase (LOC4332523), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OOa30064	XM_015774919.2 Latest version!	Oryza sativa Japonica Group 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase (LOC4332523), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
View Old Accession Versions >>
OOa07115	XM_015774920.1	Oryza sativa Japonica Group 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase (LOC4332523), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OOa30063	XM_015774918.1	Oryza sativa Japonica Group 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase (LOC4332523), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OOa30064	XM_015774919.1	Oryza sativa Japonica Group 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase (LOC4332523), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC4332523 ( XM_015774920.2 ) cDNA ORF clone, Oryza sativa Japonica Group(Japanese rice) -> XP_015630406.1 Oryza sativa Japonica Group 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase (LOC4332523), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OOa07115
Clone ID Related Accession (Same CDS sequence)	XM_015774920.2 , XM_015774920.1
Accession Version	XM_015774920.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1962bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-08-06
Organism	Oryza sativa Japonica Group(Japanese rice)
Product	6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_029258.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 7, 2018 this sequence version replaced XM_015774920.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Oryza sativa Japonica Group Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG CGGCGAGGAG CTCCGGTGGA ATGTCCGACC AGCTCTTCGT GTCCATCAAG 
CTGGAGTGCC CGCGACTCGC TGAGATGGGC CTCGTCCCGC ACGTCTTCGG CTCGCACCCA 
GTCGCCGGCG CGTGGGACCC CTCCAAGGCC CTGCTGATGG AGCAGGAGGA GGCCGCCTTG 
TGGGGGCTCA GCTGCATCGT CCCTTCTCAC CGCGAAACAC TGGATTTCAA GTTTCTATTG 
AAGCCAAAAG ACAATAGCTC ACATTGCATT GTTGAGGAGG GGCCAGACCG ATCACTAGTT 
TGTGGAAGTA ATGAGGTGGA AATTAGGAAT GCATTGTTTA AATTCAATGA TGAGACAGGT 
GTGGTTGAAT GCAAGATTTT CGTGGAGACA GAAATTTTGT CCCCATTTGA CCTAGCTGCT 
AGTTGGAAGG CTCATCAGGA GCATCTCCAG CCGAGGGTGC GAGGGGCCCA TGATGTCATT 
ATGAATGCCG ATTCTGAATC CAGGGCCAAG GGTGCAGATG GGAGACATGT GCCTCCACAG 
GAGGAACAAA GGGCGATCTT TGTTGATAGA GGTGTTGGCT CACCAGAATT TGCAAGACCC 
ACAAATGAAA CAATTTCAAT GAGCAACATC AAGTTTGATT CTGAAGCGAA GGACATGCCA 
GCAGCTGAAG GAGCTGTTGC AGCAGCAGCT GTAGCTGATC AAATGTATGG CCCAAAGGAG 
GATCGCAAGT TGGCAATTGT GCTGGTTGGG TTGCCAGCTC GTGGTAAGAC CTTTACAGCG 
GCTAAGCTTA CAAGGTACCT TCGATGGTTA GGTCATGAAA CAAAACACTT CAATGTTGGA 
AAGTACCGCC GACTCAAGCA TGGCATTAAT CAGACTGCTG ATTTCTTCCG TGGAGACAAC 
AAAGAAGGTG TGGAGGCACG CAATGAGGTG GCGGCTCTAG CAATGGAGGA TATGCTATCT 
TGGATGCAGG AAGGTGGTCA GGTAGGTATC TTTGATGCCA CAAACAGCAC AAGAAACCGG 
AGAAATATGC TGATGAAAAT GGCGGAAGGC AAGTGTAAGA TCATCTTTTT GGAAACAATA 
TGTAATGACC AAAATGTTCT CGAGAGAAAT ATGCGCTTGA AAGTTCAACG AAGTCCTGAT 
TATGCGGAAC AGACAGATTT TGAAGCTGGT GTTCGAGATT TCAAAGAGAG ATTGGCCTAT 
TATGAAAAGG TATACGAACC AGTGGAAGAA GGCTCATATG TTAAGATGAT TGATATGGTC 
AGTGGGACTG GGGGCCAATT GCAGATCAAT GATATAAGTG GTTACTTGCC TGGCCGGATT 
GTTTTCTTCT TGGTGAACTG TCATCTTACA CCTCGTCCCA TCCTGCTAAC TAGACACGGT 
GAAAGTTTAG ATAATGTCAG AGGACGGATT GGTGGAGATT CATCTTTGAG TGAAACCGGA 
TCGCTTTATT CAAGGAAACT TGCCAGCTTT ATAGAAAAGC GGCTAGCGTC TGAGAGAACT 
GCATCTATAT GGACTAGCAC ACTCCAGAGG TCAATATTAA CAGCGCAGCC TATCATTGGA 
TTTCCAAAGA TACAATGGCG TGCACTTGAT GAAATAAACG CTGGCATCTG TGATGGGATG 
ACGTATGATG AAATAAAGAA AATTAAACCT GAAGAATATG AATCACGTAG CAAGGACAAG 
CTGAGGTATC GTTATCCTAG AGGAGAATCC TACCTCGATG TCATTCAAAG ATTAGAACCT 
GTAATTATTG AGCTTGAACG GCAGCGTGCT CCAGTTGTTG TCATATCTCA CCAGGCTGTG 
CTAAGAGCAC TATATGCATA TTTTGCGGAT AAACCACTGG AGGAACTTCC TAACATTGAG 
ATACCACTTC ATACAATAAT TGAGATACAA ATGGGTGTTG CTGGTGTTCA AGAGAAAAGA 
TACAAACTCA TGGACGCAGT GAACTCCACA GCAGGGATTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015630406.1
CDS	156..2117
Translation	MAAAARSSGGMSDQLFVSIKLECPRLAEMGLVPHVFGSHPVAGAWDPSKALLMEQEEAALWGLSCIVPSHRETLDFKFLLKPKDNSSHCIVEEGPDRSLVCGSNEVEIRNALFKFNDETGVVECKIFVETEILSPFDLAASWKAHQEHLQPRVRGAHDVIMNADSESRAKGADGRHVPPQEEQRAIFVDRGVGSPEFARPTNETISMSNIKFDSEAKDMPAAEGAVAAAAVADQMYGPKEDRKLAIVLVGLPARGKTFTAAKLTRYLRWLGHETKHFNVGKYRRLKHGINQTADFFRGDNKEGVEARNEVAALAMEDMLSWMQEGGQVGIFDATNSTRNRRNMLMKMAEGKCKIIFLETICNDQNVLERNMRLKVQRSPDYAEQTDFEAGVRDFKERLAYYEKVYEPVEEGSYVKMIDMVSGTGGQLQINDISGYLPGRIVFFLVNCHLTPRPILLTRHGESLDNVRGRIGGDSSLSETGSLYSRKLASFIEKRLASERTASIWTSTLQRSILTAQPIIGFPKIQWRALDEINAGICDGMTYDEIKKIKPEEYESRSKDKLRYRYPRGESYLDVIQRLEPVIIELERQRAPVVVISHQAVLRALYAYFADKPLEELPNIEIPLHTIIEIQMGVAGVQEKRYKLMDAVNSTAGI

Target ORF information:

RefSeq Version	XM_015774920.2
Organism	Oryza sativa Japonica Group(Japanese rice)
Definition	Oryza sativa Japonica Group 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase (LOC4332523), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015774920.2

ORF Insert Sequence:

ATGGCGGCGG CGGCGAGGAG CTCCGGTGGA ATGTCCGACC AGCTCTTCGT GTCCATCAAG 
CTGGAGTGCC CGCGACTCGC TGAGATGGGC CTCGTCCCGC ACGTCTTCGG CTCGCACCCA 
GTCGCCGGCG CGTGGGACCC CTCCAAGGCC CTGCTGATGG AGCAGGAGGA GGCCGCCTTG 
TGGGGGCTCA GCTGCATCGT CCCTTCTCAC CGCGAAACAC TGGATTTCAA GTTTCTATTG 
AAGCCAAAAG ACAATAGCTC ACATTGCATT GTTGAGGAGG GGCCAGACCG ATCACTAGTT 
TGTGGAAGTA ATGAGGTGGA AATTAGGAAT GCATTGTTTA AATTCAATGA TGAGACAGGT 
GTGGTTGAAT GCAAGATTTT CGTGGAGACA GAAATTTTGT CCCCATTTGA CCTAGCTGCT 
AGTTGGAAGG CTCATCAGGA GCATCTCCAG CCGAGGGTGC GAGGGGCCCA TGATGTCATT 
ATGAATGCCG ATTCTGAATC CAGGGCCAAG GGTGCAGATG GGAGACATGT GCCTCCACAG 
GAGGAACAAA GGGCGATCTT TGTTGATAGA GGTGTTGGCT CACCAGAATT TGCAAGACCC 
ACAAATGAAA CAATTTCAAT GAGCAACATC AAGTTTGATT CTGAAGCGAA GGACATGCCA 
GCAGCTGAAG GAGCTGTTGC AGCAGCAGCT GTAGCTGATC AAATGTATGG CCCAAAGGAG 
GATCGCAAGT TGGCAATTGT GCTGGTTGGG TTGCCAGCTC GTGGTAAGAC CTTTACAGCG 
GCTAAGCTTA CAAGGTACCT TCGATGGTTA GGTCATGAAA CAAAACACTT CAATGTTGGA 
AAGTACCGCC GACTCAAGCA TGGCATTAAT CAGACTGCTG ATTTCTTCCG TGGAGACAAC 
AAAGAAGGTG TGGAGGCACG CAATGAGGTG GCGGCTCTAG CAATGGAGGA TATGCTATCT 
TGGATGCAGG AAGGTGGTCA GGTAGGTATC TTTGATGCCA CAAACAGCAC AAGAAACCGG 
AGAAATATGC TGATGAAAAT GGCGGAAGGC AAGTGTAAGA TCATCTTTTT GGAAACAATA 
TGTAATGACC AAAATGTTCT CGAGAGAAAT ATGCGCTTGA AAGTTCAACG AAGTCCTGAT 
TATGCGGAAC AGACAGATTT TGAAGCTGGT GTTCGAGATT TCAAAGAGAG ATTGGCCTAT 
TATGAAAAGG TATACGAACC AGTGGAAGAA GGCTCATATG TTAAGATGAT TGATATGGTC 
AGTGGGACTG GGGGCCAATT GCAGATCAAT GATATAAGTG GTTACTTGCC TGGCCGGATT 
GTTTTCTTCT TGGTGAACTG TCATCTTACA CCTCGTCCCA TCCTGCTAAC TAGACACGGT 
GAAAGTTTAG ATAATGTCAG AGGACGGATT GGTGGAGATT CATCTTTGAG TGAAACCGGA 
TCGCTTTATT CAAGGAAACT TGCCAGCTTT ATAGAAAAGC GGCTAGCGTC TGAGAGAACT 
GCATCTATAT GGACTAGCAC ACTCCAGAGG TCAATATTAA CAGCGCAGCC TATCATTGGA 
TTTCCAAAGA TACAATGGCG TGCACTTGAT GAAATAAACG CTGGCATCTG TGATGGGATG 
ACGTATGATG AAATAAAGAA AATTAAACCT GAAGAATATG AATCACGTAG CAAGGACAAG 
CTGAGGTATC GTTATCCTAG AGGAGAATCC TACCTCGATG TCATTCAAAG ATTAGAACCT 
GTAATTATTG AGCTTGAACG GCAGCGTGCT CCAGTTGTTG TCATATCTCA CCAGGCTGTG 
CTAAGAGCAC TATATGCATA TTTTGCGGAT AAACCACTGG AGGAACTTCC TAACATTGAG 
ATACCACTTC ATACAATAAT TGAGATACAA ATGGGTGTTG CTGGTGTTCA AGAGAAAAGA 
TACAAACTCA TGGACGCAGT GAACTCCACA GCAGGGATTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC4332523 ( XM_015774920.1 ) cDNA ORF clone, Oryza sativa Japonica Group(Japanese rice) -> XP_015630406.1 Oryza sativa Japonica Group 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase (LOC4332523), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OOa07115
Clone ID Related Accession (Same CDS sequence)	XM_015774920.2 , XM_015774920.1
Accession Version	XM_015774920.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1962bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-02-29
Organism	Oryza sativa Japonica Group(Japanese rice)
Product	6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015379176.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oryza sativa Japonica Group Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG CGGCGAGGAG CTCCGGTGGA ATGTCCGACC AGCTCTTCGT GTCCATCAAG 
CTGGAGTGCC CGCGACTCGC TGAGATGGGC CTCGTCCCGC ACGTCTTCGG CTCGCACCCA 
GTCGCCGGCG CGTGGGACCC CTCCAAGGCC CTGCTGATGG AGCAGGAGGA GGCCGCCTTG 
TGGGGGCTCA GCTGCATCGT CCCTTCTCAC CGCGAAACAC TGGATTTCAA GTTTCTATTG 
AAGCCAAAAG ACAATAGCTC ACATTGCATT GTTGAGGAGG GGCCAGACCG ATCACTAGTT 
TGTGGAAGTA ATGAGGTGGA AATTAGGAAT GCATTGTTTA AATTCAATGA TGAGACAGGT 
GTGGTTGAAT GCAAGATTTT CGTGGAGACA GAAATTTTGT CCCCATTTGA CCTAGCTGCT 
AGTTGGAAGG CTCATCAGGA GCATCTCCAG CCGAGGGTGC GAGGGGCCCA TGATGTCATT 
ATGAATGCCG ATTCTGAATC CAGGGCCAAG GGTGCAGATG GGAGACATGT GCCTCCACAG 
GAGGAACAAA GGGCGATCTT TGTTGATAGA GGTGTTGGCT CACCAGAATT TGCAAGACCC 
ACAAATGAAA CAATTTCAAT GAGCAACATC AAGTTTGATT CTGAAGCGAA GGACATGCCA 
GCAGCTGAAG GAGCTGTTGC AGCAGCAGCT GTAGCTGATC AAATGTATGG CCCAAAGGAG 
GATCGCAAGT TGGCAATTGT GCTGGTTGGG TTGCCAGCTC GTGGTAAGAC CTTTACAGCG 
GCTAAGCTTA CAAGGTACCT TCGATGGTTA GGTCATGAAA CAAAACACTT CAATGTTGGA 
AAGTACCGCC GACTCAAGCA TGGCATTAAT CAGACTGCTG ATTTCTTCCG TGGAGACAAC 
AAAGAAGGTG TGGAGGCACG CAATGAGGTG GCGGCTCTAG CAATGGAGGA TATGCTATCT 
TGGATGCAGG AAGGTGGTCA GGTAGGTATC TTTGATGCCA CAAACAGCAC AAGAAACCGG 
AGAAATATGC TGATGAAAAT GGCGGAAGGC AAGTGTAAGA TCATCTTTTT GGAAACAATA 
TGTAATGACC AAAATGTTCT CGAGAGAAAT ATGCGCTTGA AAGTTCAACG AAGTCCTGAT 
TATGCGGAAC AGACAGATTT TGAAGCTGGT GTTCGAGATT TCAAAGAGAG ATTGGCCTAT 
TATGAAAAGG TATACGAACC AGTGGAAGAA GGCTCATATG TTAAGATGAT TGATATGGTC 
AGTGGGACTG GGGGCCAATT GCAGATCAAT GATATAAGTG GTTACTTGCC TGGCCGGATT 
GTTTTCTTCT TGGTGAACTG TCATCTTACA CCTCGTCCCA TCCTGCTAAC TAGACACGGT 
GAAAGTTTAG ATAATGTCAG AGGACGGATT GGTGGAGATT CATCTTTGAG TGAAACCGGA 
TCGCTTTATT CAAGGAAACT TGCCAGCTTT ATAGAAAAGC GGCTAGCGTC TGAGAGAACT 
GCATCTATAT GGACTAGCAC ACTCCAGAGG TCAATATTAA CAGCGCAGCC TATCATTGGA 
TTTCCAAAGA TACAATGGCG TGCACTTGAT GAAATAAACG CTGGCATCTG TGATGGGATG 
ACGTATGATG AAATAAAGAA AATTAAACCT GAAGAATATG AATCACGTAG CAAGGACAAG 
CTGAGGTATC GTTATCCTAG AGGAGAATCC TACCTCGATG TCATTCAAAG ATTAGAACCT 
GTAATTATTG AGCTTGAACG GCAGCGTGCT CCAGTTGTTG TCATATCTCA CCAGGCTGTG 
CTAAGAGCAC TATATGCATA TTTTGCGGAT AAACCACTGG AGGAACTTCC TAACATTGAG 
ATACCACTTC ATACAATAAT TGAGATACAA ATGGGTGTTG CTGGTGTTCA AGAGAAAAGA 
TACAAACTCA TGGACGCAGT GAACTCCACA GCAGGGATTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015630406.1
CDS	155..2116
Translation	MAAAARSSGGMSDQLFVSIKLECPRLAEMGLVPHVFGSHPVAGAWDPSKALLMEQEEAALWGLSCIVPSHRETLDFKFLLKPKDNSSHCIVEEGPDRSLVCGSNEVEIRNALFKFNDETGVVECKIFVETEILSPFDLAASWKAHQEHLQPRVRGAHDVIMNADSESRAKGADGRHVPPQEEQRAIFVDRGVGSPEFARPTNETISMSNIKFDSEAKDMPAAEGAVAAAAVADQMYGPKEDRKLAIVLVGLPARGKTFTAAKLTRYLRWLGHETKHFNVGKYRRLKHGINQTADFFRGDNKEGVEARNEVAALAMEDMLSWMQEGGQVGIFDATNSTRNRRNMLMKMAEGKCKIIFLETICNDQNVLERNMRLKVQRSPDYAEQTDFEAGVRDFKERLAYYEKVYEPVEEGSYVKMIDMVSGTGGQLQINDISGYLPGRIVFFLVNCHLTPRPILLTRHGESLDNVRGRIGGDSSLSETGSLYSRKLASFIEKRLASERTASIWTSTLQRSILTAQPIIGFPKIQWRALDEINAGICDGMTYDEIKKIKPEEYESRSKDKLRYRYPRGESYLDVIQRLEPVIIELERQRAPVVVISHQAVLRALYAYFADKPLEELPNIEIPLHTIIEIQMGVAGVQEKRYKLMDAVNSTAGI

Target ORF information:

RefSeq Version	XM_015774920.1
Organism	Oryza sativa Japonica Group(Japanese rice)
Definition	Oryza sativa Japonica Group 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase (LOC4332523), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015774920.1

ORF Insert Sequence:

ATGGCGGCGG CGGCGAGGAG CTCCGGTGGA ATGTCCGACC AGCTCTTCGT GTCCATCAAG 
CTGGAGTGCC CGCGACTCGC TGAGATGGGC CTCGTCCCGC ACGTCTTCGG CTCGCACCCA 
GTCGCCGGCG CGTGGGACCC CTCCAAGGCC CTGCTGATGG AGCAGGAGGA GGCCGCCTTG 
TGGGGGCTCA GCTGCATCGT CCCTTCTCAC CGCGAAACAC TGGATTTCAA GTTTCTATTG 
AAGCCAAAAG ACAATAGCTC ACATTGCATT GTTGAGGAGG GGCCAGACCG ATCACTAGTT 
TGTGGAAGTA ATGAGGTGGA AATTAGGAAT GCATTGTTTA AATTCAATGA TGAGACAGGT 
GTGGTTGAAT GCAAGATTTT CGTGGAGACA GAAATTTTGT CCCCATTTGA CCTAGCTGCT 
AGTTGGAAGG CTCATCAGGA GCATCTCCAG CCGAGGGTGC GAGGGGCCCA TGATGTCATT 
ATGAATGCCG ATTCTGAATC CAGGGCCAAG GGTGCAGATG GGAGACATGT GCCTCCACAG 
GAGGAACAAA GGGCGATCTT TGTTGATAGA GGTGTTGGCT CACCAGAATT TGCAAGACCC 
ACAAATGAAA CAATTTCAAT GAGCAACATC AAGTTTGATT CTGAAGCGAA GGACATGCCA 
GCAGCTGAAG GAGCTGTTGC AGCAGCAGCT GTAGCTGATC AAATGTATGG CCCAAAGGAG 
GATCGCAAGT TGGCAATTGT GCTGGTTGGG TTGCCAGCTC GTGGTAAGAC CTTTACAGCG 
GCTAAGCTTA CAAGGTACCT TCGATGGTTA GGTCATGAAA CAAAACACTT CAATGTTGGA 
AAGTACCGCC GACTCAAGCA TGGCATTAAT CAGACTGCTG ATTTCTTCCG TGGAGACAAC 
AAAGAAGGTG TGGAGGCACG CAATGAGGTG GCGGCTCTAG CAATGGAGGA TATGCTATCT 
TGGATGCAGG AAGGTGGTCA GGTAGGTATC TTTGATGCCA CAAACAGCAC AAGAAACCGG 
AGAAATATGC TGATGAAAAT GGCGGAAGGC AAGTGTAAGA TCATCTTTTT GGAAACAATA 
TGTAATGACC AAAATGTTCT CGAGAGAAAT ATGCGCTTGA AAGTTCAACG AAGTCCTGAT 
TATGCGGAAC AGACAGATTT TGAAGCTGGT GTTCGAGATT TCAAAGAGAG ATTGGCCTAT 
TATGAAAAGG TATACGAACC AGTGGAAGAA GGCTCATATG TTAAGATGAT TGATATGGTC 
AGTGGGACTG GGGGCCAATT GCAGATCAAT GATATAAGTG GTTACTTGCC TGGCCGGATT 
GTTTTCTTCT TGGTGAACTG TCATCTTACA CCTCGTCCCA TCCTGCTAAC TAGACACGGT 
GAAAGTTTAG ATAATGTCAG AGGACGGATT GGTGGAGATT CATCTTTGAG TGAAACCGGA 
TCGCTTTATT CAAGGAAACT TGCCAGCTTT ATAGAAAAGC GGCTAGCGTC TGAGAGAACT 
GCATCTATAT GGACTAGCAC ACTCCAGAGG TCAATATTAA CAGCGCAGCC TATCATTGGA 
TTTCCAAAGA TACAATGGCG TGCACTTGAT GAAATAAACG CTGGCATCTG TGATGGGATG 
ACGTATGATG AAATAAAGAA AATTAAACCT GAAGAATATG AATCACGTAG CAAGGACAAG 
CTGAGGTATC GTTATCCTAG AGGAGAATCC TACCTCGATG TCATTCAAAG ATTAGAACCT 
GTAATTATTG AGCTTGAACG GCAGCGTGCT CCAGTTGTTG TCATATCTCA CCAGGCTGTG 
CTAAGAGCAC TATATGCATA TTTTGCGGAT AAACCACTGG AGGAACTTCC TAACATTGAG 
ATACCACTTC ATACAATAAT TGAGATACAA ATGGGTGTTG CTGGTGTTCA AGAGAAAAGA 
TACAAACTCA TGGACGCAGT GAACTCCACA GCAGGGATTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC4332523 ( XM_015774918.1 ) cDNA ORF clone, Oryza sativa Japonica Group(Japanese rice) -> XP_015630404.1 Oryza sativa Japonica Group 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase (LOC4332523), transcript variant X1, mRNA.

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CloneID	OOa30063
Clone ID Related Accession (Same CDS sequence)	XM_015774918.1 , XM_015774918.2
Accession Version	XM_015774918.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2181bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-02-29
Organism	Oryza sativa Japonica Group(Japanese rice)
Product	6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015379176.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oryza sativa Japonica Group Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG CGGCGAGGAG CTCCGGTGGA ATGTCCGACC AGCTCTTCGT GTCCATCAAG 
CTGGAGTGCC CGCGACTCGC TGAGATGGGC CTCGTCCCGC ACGTCTTCGG CTCGCACCCA 
GTCGCCGGCG CGTGGGACCC CTCCAAGGCC CTGCTGATGG AGCAGGAGGA GGCCGCCTTG 
TGGGGGCTCA GCTGCATCGT CCCTTCTCAC CGCGAAACAC TGGATTTCAA GTTTCTATTG 
AAGCCAAAAG ACAATAGCTC ACATTGCATT GTTGAGGAGG GGCCAGACCG ATCACTAGTT 
TGTGGAAGTA ATGAGGTGGA AATTAGGAAT GCATTGTTTA AATTCAATGA TGAGACAGGT 
GTGGTTGAAT GCAAGATTTT CGTGGAGACA GAAATTTTGT CCCCATTTGA CCTAGCTGCT 
AGTTGGAAGG CTCATCAGGA GCATCTCCAG CCGAGGGTGC GAGGGGCCCA TGATGTCATT 
ATGAATGCCG ATTCTGAATC CAGGGCCAAG AATGGCTTCG CTTCTGGTTT GGAGCTTGAT 
TTAGAGAAGT ATGTAGTTCC GACACCAAAC ATGGGCTCGG ATGTTGTTTA TGCAGCTAAC 
TTGACTGAAA ATCCCCGCTC GTTATTGACT ACGGGGATTT CCTCAAACAA TGATACCACA 
AAGGGCTTTT TGCACAACTC GATCAAAGGC GATGCATCCC CGAACCTCTA TGCTAGTGAA 
AACAAGGGTG CAGATGGGAG ACATGTGCCT CCACAGGAGG AACAAAGGGC GATCTTTGTT 
GATAGAGGTG TTGGCTCACC AGAATTTGCA AGACCCACAA ATGAAACAAT TTCAATGAGC 
AACATCAAGT TTGATTCTGA AGCGAAGCAG GACATGCCAG CAGCTGAAGG AGCTGTTGCA 
GCAGCAGCTG TAGCTGATCA AATGTATGGC CCAAAGGAGG ATCGCAAGTT GGCAATTGTG 
CTGGTTGGGT TGCCAGCTCG TGGTAAGACC TTTACAGCGG CTAAGCTTAC AAGGTACCTT 
CGATGGTTAG GTCATGAAAC AAAACACTTC AATGTTGGAA AGTACCGCCG ACTCAAGCAT 
GGCATTAATC AGACTGCTGA TTTCTTCCGT GGAGACAACA AAGAAGGTGT GGAGGCACGC 
AATGAGGTGG CGGCTCTAGC AATGGAGGAT ATGCTATCTT GGATGCAGGA AGGTGGTCAG 
GTAGGTATCT TTGATGCCAC AAACAGCACA AGAAACCGGA GAAATATGCT GATGAAAATG 
GCGGAAGGCA AGTGTAAGAT CATCTTTTTG GAAACAATAT GTAATGACCA AAATGTTCTC 
GAGAGAAATA TGCGCTTGAA AGTTCAACGA AGTCCTGATT ATGCGGAACA GACAGATTTT 
GAAGCTGGTG TTCGAGATTT CAAAGAGAGA TTGGCCTATT ATGAAAAGGT ATACGAACCA 
GTGGAAGAAG GCTCATATGT TAAGATGATT GATATGGTCA GTGGGACTGG GGGCCAATTG 
CAGATCAATG ATATAAGTGG TTACTTGCCT GGCCGGATTG TTTTCTTCTT GGTGAACTGT 
CATCTTACAC CTCGTCCCAT CCTGCTAACT AGACACGGTG AAAGTTTAGA TAATGTCAGA 
GGACGGATTG GTGGAGATTC ATCTTTGAGT GAAACCGGAT CGCTTTATTC AAGGAAACTT 
GCCAGCTTTA TAGAAAAGCG GCTAGCGTCT GAGAGAACTG CATCTATATG GACTAGCACA 
CTCCAGAGGT CAATATTAAC AGCGCAGCCT ATCATTGGAT TTCCAAAGAT ACAATGGCGT 
GCACTTGATG AAATAAACGC TGGCATCTGT GATGGGATGA CGTATGATGA AATAAAGAAA 
ATTAAACCTG AAGAATATGA ATCACGTAGC AAGGACAAGC TGAGGTATCG TTATCCTAGA 
GGAGAATCCT ACCTCGATGT CATTCAAAGA TTAGAACCTG TAATTATTGA GCTTGAACGG 
CAGCGTGCTC CAGTTGTTGT CATATCTCAC CAGGCTGTGC TAAGAGCACT ATATGCATAT 
TTTGCGGATA AACCACTGGA GGAACTTCCT AACATTGAGA TACCACTTCA TACAATAATT 
GAGATACAAA TGGGTGTTGC TGGTGTTCAA GAGAAAAGAT ACAAACTCAT GGACGCAGTG 
AACTCCACAG CAGGGATTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015630404.1
CDS	165..2345
Translation	MAAAARSSGGMSDQLFVSIKLECPRLAEMGLVPHVFGSHPVAGAWDPSKALLMEQEEAALWGLSCIVPSHRETLDFKFLLKPKDNSSHCIVEEGPDRSLVCGSNEVEIRNALFKFNDETGVVECKIFVETEILSPFDLAASWKAHQEHLQPRVRGAHDVIMNADSESRAKNGFASGLELDLEKYVVPTPNMGSDVVYAANLTENPRSLLTTGISSNNDTTKGFLHNSIKGDASPNLYASENKGADGRHVPPQEEQRAIFVDRGVGSPEFARPTNETISMSNIKFDSEAKQDMPAAEGAVAAAAVADQMYGPKEDRKLAIVLVGLPARGKTFTAAKLTRYLRWLGHETKHFNVGKYRRLKHGINQTADFFRGDNKEGVEARNEVAALAMEDMLSWMQEGGQVGIFDATNSTRNRRNMLMKMAEGKCKIIFLETICNDQNVLERNMRLKVQRSPDYAEQTDFEAGVRDFKERLAYYEKVYEPVEEGSYVKMIDMVSGTGGQLQINDISGYLPGRIVFFLVNCHLTPRPILLTRHGESLDNVRGRIGGDSSLSETGSLYSRKLASFIEKRLASERTASIWTSTLQRSILTAQPIIGFPKIQWRALDEINAGICDGMTYDEIKKIKPEEYESRSKDKLRYRYPRGESYLDVIQRLEPVIIELERQRAPVVVISHQAVLRALYAYFADKPLEELPNIEIPLHTIIEIQMGVAGVQEKRYKLMDAVNSTAGI

Target ORF information:

RefSeq Version	XM_015774918.1
Organism	Oryza sativa Japonica Group(Japanese rice)
Definition	Oryza sativa Japonica Group 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase (LOC4332523), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015774918.1

ORF Insert Sequence:

ATGGCGGCGG CGGCGAGGAG CTCCGGTGGA ATGTCCGACC AGCTCTTCGT GTCCATCAAG 
CTGGAGTGCC CGCGACTCGC TGAGATGGGC CTCGTCCCGC ACGTCTTCGG CTCGCACCCA 
GTCGCCGGCG CGTGGGACCC CTCCAAGGCC CTGCTGATGG AGCAGGAGGA GGCCGCCTTG 
TGGGGGCTCA GCTGCATCGT CCCTTCTCAC CGCGAAACAC TGGATTTCAA GTTTCTATTG 
AAGCCAAAAG ACAATAGCTC ACATTGCATT GTTGAGGAGG GGCCAGACCG ATCACTAGTT 
TGTGGAAGTA ATGAGGTGGA AATTAGGAAT GCATTGTTTA AATTCAATGA TGAGACAGGT 
GTGGTTGAAT GCAAGATTTT CGTGGAGACA GAAATTTTGT CCCCATTTGA CCTAGCTGCT 
AGTTGGAAGG CTCATCAGGA GCATCTCCAG CCGAGGGTGC GAGGGGCCCA TGATGTCATT 
ATGAATGCCG ATTCTGAATC CAGGGCCAAG AATGGCTTCG CTTCTGGTTT GGAGCTTGAT 
TTAGAGAAGT ATGTAGTTCC GACACCAAAC ATGGGCTCGG ATGTTGTTTA TGCAGCTAAC 
TTGACTGAAA ATCCCCGCTC GTTATTGACT ACGGGGATTT CCTCAAACAA TGATACCACA 
AAGGGCTTTT TGCACAACTC GATCAAAGGC GATGCATCCC CGAACCTCTA TGCTAGTGAA 
AACAAGGGTG CAGATGGGAG ACATGTGCCT CCACAGGAGG AACAAAGGGC GATCTTTGTT 
GATAGAGGTG TTGGCTCACC AGAATTTGCA AGACCCACAA ATGAAACAAT TTCAATGAGC 
AACATCAAGT TTGATTCTGA AGCGAAGCAG GACATGCCAG CAGCTGAAGG AGCTGTTGCA 
GCAGCAGCTG TAGCTGATCA AATGTATGGC CCAAAGGAGG ATCGCAAGTT GGCAATTGTG 
CTGGTTGGGT TGCCAGCTCG TGGTAAGACC TTTACAGCGG CTAAGCTTAC AAGGTACCTT 
CGATGGTTAG GTCATGAAAC AAAACACTTC AATGTTGGAA AGTACCGCCG ACTCAAGCAT 
GGCATTAATC AGACTGCTGA TTTCTTCCGT GGAGACAACA AAGAAGGTGT GGAGGCACGC 
AATGAGGTGG CGGCTCTAGC AATGGAGGAT ATGCTATCTT GGATGCAGGA AGGTGGTCAG 
GTAGGTATCT TTGATGCCAC AAACAGCACA AGAAACCGGA GAAATATGCT GATGAAAATG 
GCGGAAGGCA AGTGTAAGAT CATCTTTTTG GAAACAATAT GTAATGACCA AAATGTTCTC 
GAGAGAAATA TGCGCTTGAA AGTTCAACGA AGTCCTGATT ATGCGGAACA GACAGATTTT 
GAAGCTGGTG TTCGAGATTT CAAAGAGAGA TTGGCCTATT ATGAAAAGGT ATACGAACCA 
GTGGAAGAAG GCTCATATGT TAAGATGATT GATATGGTCA GTGGGACTGG GGGCCAATTG 
CAGATCAATG ATATAAGTGG TTACTTGCCT GGCCGGATTG TTTTCTTCTT GGTGAACTGT 
CATCTTACAC CTCGTCCCAT CCTGCTAACT AGACACGGTG AAAGTTTAGA TAATGTCAGA 
GGACGGATTG GTGGAGATTC ATCTTTGAGT GAAACCGGAT CGCTTTATTC AAGGAAACTT 
GCCAGCTTTA TAGAAAAGCG GCTAGCGTCT GAGAGAACTG CATCTATATG GACTAGCACA 
CTCCAGAGGT CAATATTAAC AGCGCAGCCT ATCATTGGAT TTCCAAAGAT ACAATGGCGT 
GCACTTGATG AAATAAACGC TGGCATCTGT GATGGGATGA CGTATGATGA AATAAAGAAA 
ATTAAACCTG AAGAATATGA ATCACGTAGC AAGGACAAGC TGAGGTATCG TTATCCTAGA 
GGAGAATCCT ACCTCGATGT CATTCAAAGA TTAGAACCTG TAATTATTGA GCTTGAACGG 
CAGCGTGCTC CAGTTGTTGT CATATCTCAC CAGGCTGTGC TAAGAGCACT ATATGCATAT 
TTTGCGGATA AACCACTGGA GGAACTTCCT AACATTGAGA TACCACTTCA TACAATAATT 
GAGATACAAA TGGGTGTTGC TGGTGTTCAA GAGAAAAGAT ACAAACTCAT GGACGCAGTG 
AACTCCACAG CAGGGATTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC4332523 ( XM_015774918.2 ) cDNA ORF clone, Oryza sativa Japonica Group(Japanese rice) -> XP_015630404.1 Oryza sativa Japonica Group 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase (LOC4332523), transcript variant X1, mRNA.

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CloneID	OOa30063
Clone ID Related Accession (Same CDS sequence)	XM_015774918.1 , XM_015774918.2
Accession Version	XM_015774918.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2181bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-08-06
Organism	Oryza sativa Japonica Group(Japanese rice)
Product	6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_029258.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 7, 2018 this sequence version replaced XM_015774918.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Oryza sativa Japonica Group Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG CGGCGAGGAG CTCCGGTGGA ATGTCCGACC AGCTCTTCGT GTCCATCAAG 
CTGGAGTGCC CGCGACTCGC TGAGATGGGC CTCGTCCCGC ACGTCTTCGG CTCGCACCCA 
GTCGCCGGCG CGTGGGACCC CTCCAAGGCC CTGCTGATGG AGCAGGAGGA GGCCGCCTTG 
TGGGGGCTCA GCTGCATCGT CCCTTCTCAC CGCGAAACAC TGGATTTCAA GTTTCTATTG 
AAGCCAAAAG ACAATAGCTC ACATTGCATT GTTGAGGAGG GGCCAGACCG ATCACTAGTT 
TGTGGAAGTA ATGAGGTGGA AATTAGGAAT GCATTGTTTA AATTCAATGA TGAGACAGGT 
GTGGTTGAAT GCAAGATTTT CGTGGAGACA GAAATTTTGT CCCCATTTGA CCTAGCTGCT 
AGTTGGAAGG CTCATCAGGA GCATCTCCAG CCGAGGGTGC GAGGGGCCCA TGATGTCATT 
ATGAATGCCG ATTCTGAATC CAGGGCCAAG AATGGCTTCG CTTCTGGTTT GGAGCTTGAT 
TTAGAGAAGT ATGTAGTTCC GACACCAAAC ATGGGCTCGG ATGTTGTTTA TGCAGCTAAC 
TTGACTGAAA ATCCCCGCTC GTTATTGACT ACGGGGATTT CCTCAAACAA TGATACCACA 
AAGGGCTTTT TGCACAACTC GATCAAAGGC GATGCATCCC CGAACCTCTA TGCTAGTGAA 
AACAAGGGTG CAGATGGGAG ACATGTGCCT CCACAGGAGG AACAAAGGGC GATCTTTGTT 
GATAGAGGTG TTGGCTCACC AGAATTTGCA AGACCCACAA ATGAAACAAT TTCAATGAGC 
AACATCAAGT TTGATTCTGA AGCGAAGCAG GACATGCCAG CAGCTGAAGG AGCTGTTGCA 
GCAGCAGCTG TAGCTGATCA AATGTATGGC CCAAAGGAGG ATCGCAAGTT GGCAATTGTG 
CTGGTTGGGT TGCCAGCTCG TGGTAAGACC TTTACAGCGG CTAAGCTTAC AAGGTACCTT 
CGATGGTTAG GTCATGAAAC AAAACACTTC AATGTTGGAA AGTACCGCCG ACTCAAGCAT 
GGCATTAATC AGACTGCTGA TTTCTTCCGT GGAGACAACA AAGAAGGTGT GGAGGCACGC 
AATGAGGTGG CGGCTCTAGC AATGGAGGAT ATGCTATCTT GGATGCAGGA AGGTGGTCAG 
GTAGGTATCT TTGATGCCAC AAACAGCACA AGAAACCGGA GAAATATGCT GATGAAAATG 
GCGGAAGGCA AGTGTAAGAT CATCTTTTTG GAAACAATAT GTAATGACCA AAATGTTCTC 
GAGAGAAATA TGCGCTTGAA AGTTCAACGA AGTCCTGATT ATGCGGAACA GACAGATTTT 
GAAGCTGGTG TTCGAGATTT CAAAGAGAGA TTGGCCTATT ATGAAAAGGT ATACGAACCA 
GTGGAAGAAG GCTCATATGT TAAGATGATT GATATGGTCA GTGGGACTGG GGGCCAATTG 
CAGATCAATG ATATAAGTGG TTACTTGCCT GGCCGGATTG TTTTCTTCTT GGTGAACTGT 
CATCTTACAC CTCGTCCCAT CCTGCTAACT AGACACGGTG AAAGTTTAGA TAATGTCAGA 
GGACGGATTG GTGGAGATTC ATCTTTGAGT GAAACCGGAT CGCTTTATTC AAGGAAACTT 
GCCAGCTTTA TAGAAAAGCG GCTAGCGTCT GAGAGAACTG CATCTATATG GACTAGCACA 
CTCCAGAGGT CAATATTAAC AGCGCAGCCT ATCATTGGAT TTCCAAAGAT ACAATGGCGT 
GCACTTGATG AAATAAACGC TGGCATCTGT GATGGGATGA CGTATGATGA AATAAAGAAA 
ATTAAACCTG AAGAATATGA ATCACGTAGC AAGGACAAGC TGAGGTATCG TTATCCTAGA 
GGAGAATCCT ACCTCGATGT CATTCAAAGA TTAGAACCTG TAATTATTGA GCTTGAACGG 
CAGCGTGCTC CAGTTGTTGT CATATCTCAC CAGGCTGTGC TAAGAGCACT ATATGCATAT 
TTTGCGGATA AACCACTGGA GGAACTTCCT AACATTGAGA TACCACTTCA TACAATAATT 
GAGATACAAA TGGGTGTTGC TGGTGTTCAA GAGAAAAGAT ACAAACTCAT GGACGCAGTG 
AACTCCACAG CAGGGATTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015630404.1
CDS	163..2343
Translation	MAAAARSSGGMSDQLFVSIKLECPRLAEMGLVPHVFGSHPVAGAWDPSKALLMEQEEAALWGLSCIVPSHRETLDFKFLLKPKDNSSHCIVEEGPDRSLVCGSNEVEIRNALFKFNDETGVVECKIFVETEILSPFDLAASWKAHQEHLQPRVRGAHDVIMNADSESRAKNGFASGLELDLEKYVVPTPNMGSDVVYAANLTENPRSLLTTGISSNNDTTKGFLHNSIKGDASPNLYASENKGADGRHVPPQEEQRAIFVDRGVGSPEFARPTNETISMSNIKFDSEAKQDMPAAEGAVAAAAVADQMYGPKEDRKLAIVLVGLPARGKTFTAAKLTRYLRWLGHETKHFNVGKYRRLKHGINQTADFFRGDNKEGVEARNEVAALAMEDMLSWMQEGGQVGIFDATNSTRNRRNMLMKMAEGKCKIIFLETICNDQNVLERNMRLKVQRSPDYAEQTDFEAGVRDFKERLAYYEKVYEPVEEGSYVKMIDMVSGTGGQLQINDISGYLPGRIVFFLVNCHLTPRPILLTRHGESLDNVRGRIGGDSSLSETGSLYSRKLASFIEKRLASERTASIWTSTLQRSILTAQPIIGFPKIQWRALDEINAGICDGMTYDEIKKIKPEEYESRSKDKLRYRYPRGESYLDVIQRLEPVIIELERQRAPVVVISHQAVLRALYAYFADKPLEELPNIEIPLHTIIEIQMGVAGVQEKRYKLMDAVNSTAGI

Target ORF information:

RefSeq Version	XM_015774918.2
Organism	Oryza sativa Japonica Group(Japanese rice)
Definition	Oryza sativa Japonica Group 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase (LOC4332523), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015774918.2

ORF Insert Sequence:

ATGGCGGCGG CGGCGAGGAG CTCCGGTGGA ATGTCCGACC AGCTCTTCGT GTCCATCAAG 
CTGGAGTGCC CGCGACTCGC TGAGATGGGC CTCGTCCCGC ACGTCTTCGG CTCGCACCCA 
GTCGCCGGCG CGTGGGACCC CTCCAAGGCC CTGCTGATGG AGCAGGAGGA GGCCGCCTTG 
TGGGGGCTCA GCTGCATCGT CCCTTCTCAC CGCGAAACAC TGGATTTCAA GTTTCTATTG 
AAGCCAAAAG ACAATAGCTC ACATTGCATT GTTGAGGAGG GGCCAGACCG ATCACTAGTT 
TGTGGAAGTA ATGAGGTGGA AATTAGGAAT GCATTGTTTA AATTCAATGA TGAGACAGGT 
GTGGTTGAAT GCAAGATTTT CGTGGAGACA GAAATTTTGT CCCCATTTGA CCTAGCTGCT 
AGTTGGAAGG CTCATCAGGA GCATCTCCAG CCGAGGGTGC GAGGGGCCCA TGATGTCATT 
ATGAATGCCG ATTCTGAATC CAGGGCCAAG AATGGCTTCG CTTCTGGTTT GGAGCTTGAT 
TTAGAGAAGT ATGTAGTTCC GACACCAAAC ATGGGCTCGG ATGTTGTTTA TGCAGCTAAC 
TTGACTGAAA ATCCCCGCTC GTTATTGACT ACGGGGATTT CCTCAAACAA TGATACCACA 
AAGGGCTTTT TGCACAACTC GATCAAAGGC GATGCATCCC CGAACCTCTA TGCTAGTGAA 
AACAAGGGTG CAGATGGGAG ACATGTGCCT CCACAGGAGG AACAAAGGGC GATCTTTGTT 
GATAGAGGTG TTGGCTCACC AGAATTTGCA AGACCCACAA ATGAAACAAT TTCAATGAGC 
AACATCAAGT TTGATTCTGA AGCGAAGCAG GACATGCCAG CAGCTGAAGG AGCTGTTGCA 
GCAGCAGCTG TAGCTGATCA AATGTATGGC CCAAAGGAGG ATCGCAAGTT GGCAATTGTG 
CTGGTTGGGT TGCCAGCTCG TGGTAAGACC TTTACAGCGG CTAAGCTTAC AAGGTACCTT 
CGATGGTTAG GTCATGAAAC AAAACACTTC AATGTTGGAA AGTACCGCCG ACTCAAGCAT 
GGCATTAATC AGACTGCTGA TTTCTTCCGT GGAGACAACA AAGAAGGTGT GGAGGCACGC 
AATGAGGTGG CGGCTCTAGC AATGGAGGAT ATGCTATCTT GGATGCAGGA AGGTGGTCAG 
GTAGGTATCT TTGATGCCAC AAACAGCACA AGAAACCGGA GAAATATGCT GATGAAAATG 
GCGGAAGGCA AGTGTAAGAT CATCTTTTTG GAAACAATAT GTAATGACCA AAATGTTCTC 
GAGAGAAATA TGCGCTTGAA AGTTCAACGA AGTCCTGATT ATGCGGAACA GACAGATTTT 
GAAGCTGGTG TTCGAGATTT CAAAGAGAGA TTGGCCTATT ATGAAAAGGT ATACGAACCA 
GTGGAAGAAG GCTCATATGT TAAGATGATT GATATGGTCA GTGGGACTGG GGGCCAATTG 
CAGATCAATG ATATAAGTGG TTACTTGCCT GGCCGGATTG TTTTCTTCTT GGTGAACTGT 
CATCTTACAC CTCGTCCCAT CCTGCTAACT AGACACGGTG AAAGTTTAGA TAATGTCAGA 
GGACGGATTG GTGGAGATTC ATCTTTGAGT GAAACCGGAT CGCTTTATTC AAGGAAACTT 
GCCAGCTTTA TAGAAAAGCG GCTAGCGTCT GAGAGAACTG CATCTATATG GACTAGCACA 
CTCCAGAGGT CAATATTAAC AGCGCAGCCT ATCATTGGAT TTCCAAAGAT ACAATGGCGT 
GCACTTGATG AAATAAACGC TGGCATCTGT GATGGGATGA CGTATGATGA AATAAAGAAA 
ATTAAACCTG AAGAATATGA ATCACGTAGC AAGGACAAGC TGAGGTATCG TTATCCTAGA 
GGAGAATCCT ACCTCGATGT CATTCAAAGA TTAGAACCTG TAATTATTGA GCTTGAACGG 
CAGCGTGCTC CAGTTGTTGT CATATCTCAC CAGGCTGTGC TAAGAGCACT ATATGCATAT 
TTTGCGGATA AACCACTGGA GGAACTTCCT AACATTGAGA TACCACTTCA TACAATAATT 
GAGATACAAA TGGGTGTTGC TGGTGTTCAA GAGAAAAGAT ACAAACTCAT GGACGCAGTG 
AACTCCACAG CAGGGATTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC4332523 ( XM_015774919.1 ) cDNA ORF clone, Oryza sativa Japonica Group(Japanese rice) -> XP_015630405.1 Oryza sativa Japonica Group 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase (LOC4332523), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OOa30064
Clone ID Related Accession (Same CDS sequence)	XM_015774919.1 , XM_015774919.2
Accession Version	XM_015774919.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2178bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-02-29
Organism	Oryza sativa Japonica Group(Japanese rice)
Product	6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015379176.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oryza sativa Japonica Group Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG CGGCGAGGAG CTCCGGTGGA ATGTCCGACC AGCTCTTCGT GTCCATCAAG 
CTGGAGTGCC CGCGACTCGC TGAGATGGGC CTCGTCCCGC ACGTCTTCGG CTCGCACCCA 
GTCGCCGGCG CGTGGGACCC CTCCAAGGCC CTGCTGATGG AGCAGGAGGA GGCCGCCTTG 
TGGGGGCTCA GCTGCATCGT CCCTTCTCAC CGCGAAACAC TGGATTTCAA GTTTCTATTG 
AAGCCAAAAG ACAATAGCTC ACATTGCATT GTTGAGGAGG GGCCAGACCG ATCACTAGTT 
TGTGGAAGTA ATGAGGTGGA AATTAGGAAT GCATTGTTTA AATTCAATGA TGAGACAGGT 
GTGGTTGAAT GCAAGATTTT CGTGGAGACA GAAATTTTGT CCCCATTTGA CCTAGCTGCT 
AGTTGGAAGG CTCATCAGGA GCATCTCCAG CCGAGGGTGC GAGGGGCCCA TGATGTCATT 
ATGAATGCCG ATTCTGAATC CAGGGCCAAG AATGGCTTCG CTTCTGGTTT GGAGCTTGAT 
TTAGAGAAGT ATGTAGTTCC GACACCAAAC ATGGGCTCGG ATGTTGTTTA TGCAGCTAAC 
TTGACTGAAA ATCCCCGCTC GTTATTGACT ACGGGGATTT CCTCAAACAA TGATACCACA 
AAGGGCTTTT TGCACAACTC GATCAAAGGC GATGCATCCC CGAACCTCTA TGCTAGTGAA 
AACAAGGGTG CAGATGGGAG ACATGTGCCT CCACAGGAGG AACAAAGGGC GATCTTTGTT 
GATAGAGGTG TTGGCTCACC AGAATTTGCA AGACCCACAA ATGAAACAAT TTCAATGAGC 
AACATCAAGT TTGATTCTGA AGCGAAGGAC ATGCCAGCAG CTGAAGGAGC TGTTGCAGCA 
GCAGCTGTAG CTGATCAAAT GTATGGCCCA AAGGAGGATC GCAAGTTGGC AATTGTGCTG 
GTTGGGTTGC CAGCTCGTGG TAAGACCTTT ACAGCGGCTA AGCTTACAAG GTACCTTCGA 
TGGTTAGGTC ATGAAACAAA ACACTTCAAT GTTGGAAAGT ACCGCCGACT CAAGCATGGC 
ATTAATCAGA CTGCTGATTT CTTCCGTGGA GACAACAAAG AAGGTGTGGA GGCACGCAAT 
GAGGTGGCGG CTCTAGCAAT GGAGGATATG CTATCTTGGA TGCAGGAAGG TGGTCAGGTA 
GGTATCTTTG ATGCCACAAA CAGCACAAGA AACCGGAGAA ATATGCTGAT GAAAATGGCG 
GAAGGCAAGT GTAAGATCAT CTTTTTGGAA ACAATATGTA ATGACCAAAA TGTTCTCGAG 
AGAAATATGC GCTTGAAAGT TCAACGAAGT CCTGATTATG CGGAACAGAC AGATTTTGAA 
GCTGGTGTTC GAGATTTCAA AGAGAGATTG GCCTATTATG AAAAGGTATA CGAACCAGTG 
GAAGAAGGCT CATATGTTAA GATGATTGAT ATGGTCAGTG GGACTGGGGG CCAATTGCAG 
ATCAATGATA TAAGTGGTTA CTTGCCTGGC CGGATTGTTT TCTTCTTGGT GAACTGTCAT 
CTTACACCTC GTCCCATCCT GCTAACTAGA CACGGTGAAA GTTTAGATAA TGTCAGAGGA 
CGGATTGGTG GAGATTCATC TTTGAGTGAA ACCGGATCGC TTTATTCAAG GAAACTTGCC 
AGCTTTATAG AAAAGCGGCT AGCGTCTGAG AGAACTGCAT CTATATGGAC TAGCACACTC 
CAGAGGTCAA TATTAACAGC GCAGCCTATC ATTGGATTTC CAAAGATACA ATGGCGTGCA 
CTTGATGAAA TAAACGCTGG CATCTGTGAT GGGATGACGT ATGATGAAAT AAAGAAAATT 
AAACCTGAAG AATATGAATC ACGTAGCAAG GACAAGCTGA GGTATCGTTA TCCTAGAGGA 
GAATCCTACC TCGATGTCAT TCAAAGATTA GAACCTGTAA TTATTGAGCT TGAACGGCAG 
CGTGCTCCAG TTGTTGTCAT ATCTCACCAG GCTGTGCTAA GAGCACTATA TGCATATTTT 
GCGGATAAAC CACTGGAGGA ACTTCCTAAC ATTGAGATAC CACTTCATAC AATAATTGAG 
ATACAAATGG GTGTTGCTGG TGTTCAAGAG AAAAGATACA AACTCATGGA CGCAGTGAAC 
TCCACAGCAG GGATTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015630405.1
CDS	161..2338
Translation	MAAAARSSGGMSDQLFVSIKLECPRLAEMGLVPHVFGSHPVAGAWDPSKALLMEQEEAALWGLSCIVPSHRETLDFKFLLKPKDNSSHCIVEEGPDRSLVCGSNEVEIRNALFKFNDETGVVECKIFVETEILSPFDLAASWKAHQEHLQPRVRGAHDVIMNADSESRAKNGFASGLELDLEKYVVPTPNMGSDVVYAANLTENPRSLLTTGISSNNDTTKGFLHNSIKGDASPNLYASENKGADGRHVPPQEEQRAIFVDRGVGSPEFARPTNETISMSNIKFDSEAKDMPAAEGAVAAAAVADQMYGPKEDRKLAIVLVGLPARGKTFTAAKLTRYLRWLGHETKHFNVGKYRRLKHGINQTADFFRGDNKEGVEARNEVAALAMEDMLSWMQEGGQVGIFDATNSTRNRRNMLMKMAEGKCKIIFLETICNDQNVLERNMRLKVQRSPDYAEQTDFEAGVRDFKERLAYYEKVYEPVEEGSYVKMIDMVSGTGGQLQINDISGYLPGRIVFFLVNCHLTPRPILLTRHGESLDNVRGRIGGDSSLSETGSLYSRKLASFIEKRLASERTASIWTSTLQRSILTAQPIIGFPKIQWRALDEINAGICDGMTYDEIKKIKPEEYESRSKDKLRYRYPRGESYLDVIQRLEPVIIELERQRAPVVVISHQAVLRALYAYFADKPLEELPNIEIPLHTIIEIQMGVAGVQEKRYKLMDAVNSTAGI

Target ORF information:

RefSeq Version	XM_015774919.1
Organism	Oryza sativa Japonica Group(Japanese rice)
Definition	Oryza sativa Japonica Group 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase (LOC4332523), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015774919.1

ORF Insert Sequence:

ATGGCGGCGG CGGCGAGGAG CTCCGGTGGA ATGTCCGACC AGCTCTTCGT GTCCATCAAG 
CTGGAGTGCC CGCGACTCGC TGAGATGGGC CTCGTCCCGC ACGTCTTCGG CTCGCACCCA 
GTCGCCGGCG CGTGGGACCC CTCCAAGGCC CTGCTGATGG AGCAGGAGGA GGCCGCCTTG 
TGGGGGCTCA GCTGCATCGT CCCTTCTCAC CGCGAAACAC TGGATTTCAA GTTTCTATTG 
AAGCCAAAAG ACAATAGCTC ACATTGCATT GTTGAGGAGG GGCCAGACCG ATCACTAGTT 
TGTGGAAGTA ATGAGGTGGA AATTAGGAAT GCATTGTTTA AATTCAATGA TGAGACAGGT 
GTGGTTGAAT GCAAGATTTT CGTGGAGACA GAAATTTTGT CCCCATTTGA CCTAGCTGCT 
AGTTGGAAGG CTCATCAGGA GCATCTCCAG CCGAGGGTGC GAGGGGCCCA TGATGTCATT 
ATGAATGCCG ATTCTGAATC CAGGGCCAAG AATGGCTTCG CTTCTGGTTT GGAGCTTGAT 
TTAGAGAAGT ATGTAGTTCC GACACCAAAC ATGGGCTCGG ATGTTGTTTA TGCAGCTAAC 
TTGACTGAAA ATCCCCGCTC GTTATTGACT ACGGGGATTT CCTCAAACAA TGATACCACA 
AAGGGCTTTT TGCACAACTC GATCAAAGGC GATGCATCCC CGAACCTCTA TGCTAGTGAA 
AACAAGGGTG CAGATGGGAG ACATGTGCCT CCACAGGAGG AACAAAGGGC GATCTTTGTT 
GATAGAGGTG TTGGCTCACC AGAATTTGCA AGACCCACAA ATGAAACAAT TTCAATGAGC 
AACATCAAGT TTGATTCTGA AGCGAAGGAC ATGCCAGCAG CTGAAGGAGC TGTTGCAGCA 
GCAGCTGTAG CTGATCAAAT GTATGGCCCA AAGGAGGATC GCAAGTTGGC AATTGTGCTG 
GTTGGGTTGC CAGCTCGTGG TAAGACCTTT ACAGCGGCTA AGCTTACAAG GTACCTTCGA 
TGGTTAGGTC ATGAAACAAA ACACTTCAAT GTTGGAAAGT ACCGCCGACT CAAGCATGGC 
ATTAATCAGA CTGCTGATTT CTTCCGTGGA GACAACAAAG AAGGTGTGGA GGCACGCAAT 
GAGGTGGCGG CTCTAGCAAT GGAGGATATG CTATCTTGGA TGCAGGAAGG TGGTCAGGTA 
GGTATCTTTG ATGCCACAAA CAGCACAAGA AACCGGAGAA ATATGCTGAT GAAAATGGCG 
GAAGGCAAGT GTAAGATCAT CTTTTTGGAA ACAATATGTA ATGACCAAAA TGTTCTCGAG 
AGAAATATGC GCTTGAAAGT TCAACGAAGT CCTGATTATG CGGAACAGAC AGATTTTGAA 
GCTGGTGTTC GAGATTTCAA AGAGAGATTG GCCTATTATG AAAAGGTATA CGAACCAGTG 
GAAGAAGGCT CATATGTTAA GATGATTGAT ATGGTCAGTG GGACTGGGGG CCAATTGCAG 
ATCAATGATA TAAGTGGTTA CTTGCCTGGC CGGATTGTTT TCTTCTTGGT GAACTGTCAT 
CTTACACCTC GTCCCATCCT GCTAACTAGA CACGGTGAAA GTTTAGATAA TGTCAGAGGA 
CGGATTGGTG GAGATTCATC TTTGAGTGAA ACCGGATCGC TTTATTCAAG GAAACTTGCC 
AGCTTTATAG AAAAGCGGCT AGCGTCTGAG AGAACTGCAT CTATATGGAC TAGCACACTC 
CAGAGGTCAA TATTAACAGC GCAGCCTATC ATTGGATTTC CAAAGATACA ATGGCGTGCA 
CTTGATGAAA TAAACGCTGG CATCTGTGAT GGGATGACGT ATGATGAAAT AAAGAAAATT 
AAACCTGAAG AATATGAATC ACGTAGCAAG GACAAGCTGA GGTATCGTTA TCCTAGAGGA 
GAATCCTACC TCGATGTCAT TCAAAGATTA GAACCTGTAA TTATTGAGCT TGAACGGCAG 
CGTGCTCCAG TTGTTGTCAT ATCTCACCAG GCTGTGCTAA GAGCACTATA TGCATATTTT 
GCGGATAAAC CACTGGAGGA ACTTCCTAAC ATTGAGATAC CACTTCATAC AATAATTGAG 
ATACAAATGG GTGTTGCTGG TGTTCAAGAG AAAAGATACA AACTCATGGA CGCAGTGAAC 
TCCACAGCAG GGATTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC4332523 ( XM_015774919.2 ) cDNA ORF clone, Oryza sativa Japonica Group(Japanese rice) -> XP_015630405.1 Oryza sativa Japonica Group 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase (LOC4332523), transcript variant X2, mRNA.

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CloneID	OOa30064
Clone ID Related Accession (Same CDS sequence)	XM_015774919.1 , XM_015774919.2
Accession Version	XM_015774919.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2178bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-08-06
Organism	Oryza sativa Japonica Group(Japanese rice)
Product	6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_029258.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 7, 2018 this sequence version replaced XM_015774919.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Oryza sativa Japonica Group Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG CGGCGAGGAG CTCCGGTGGA ATGTCCGACC AGCTCTTCGT GTCCATCAAG 
CTGGAGTGCC CGCGACTCGC TGAGATGGGC CTCGTCCCGC ACGTCTTCGG CTCGCACCCA 
GTCGCCGGCG CGTGGGACCC CTCCAAGGCC CTGCTGATGG AGCAGGAGGA GGCCGCCTTG 
TGGGGGCTCA GCTGCATCGT CCCTTCTCAC CGCGAAACAC TGGATTTCAA GTTTCTATTG 
AAGCCAAAAG ACAATAGCTC ACATTGCATT GTTGAGGAGG GGCCAGACCG ATCACTAGTT 
TGTGGAAGTA ATGAGGTGGA AATTAGGAAT GCATTGTTTA AATTCAATGA TGAGACAGGT 
GTGGTTGAAT GCAAGATTTT CGTGGAGACA GAAATTTTGT CCCCATTTGA CCTAGCTGCT 
AGTTGGAAGG CTCATCAGGA GCATCTCCAG CCGAGGGTGC GAGGGGCCCA TGATGTCATT 
ATGAATGCCG ATTCTGAATC CAGGGCCAAG AATGGCTTCG CTTCTGGTTT GGAGCTTGAT 
TTAGAGAAGT ATGTAGTTCC GACACCAAAC ATGGGCTCGG ATGTTGTTTA TGCAGCTAAC 
TTGACTGAAA ATCCCCGCTC GTTATTGACT ACGGGGATTT CCTCAAACAA TGATACCACA 
AAGGGCTTTT TGCACAACTC GATCAAAGGC GATGCATCCC CGAACCTCTA TGCTAGTGAA 
AACAAGGGTG CAGATGGGAG ACATGTGCCT CCACAGGAGG AACAAAGGGC GATCTTTGTT 
GATAGAGGTG TTGGCTCACC AGAATTTGCA AGACCCACAA ATGAAACAAT TTCAATGAGC 
AACATCAAGT TTGATTCTGA AGCGAAGGAC ATGCCAGCAG CTGAAGGAGC TGTTGCAGCA 
GCAGCTGTAG CTGATCAAAT GTATGGCCCA AAGGAGGATC GCAAGTTGGC AATTGTGCTG 
GTTGGGTTGC CAGCTCGTGG TAAGACCTTT ACAGCGGCTA AGCTTACAAG GTACCTTCGA 
TGGTTAGGTC ATGAAACAAA ACACTTCAAT GTTGGAAAGT ACCGCCGACT CAAGCATGGC 
ATTAATCAGA CTGCTGATTT CTTCCGTGGA GACAACAAAG AAGGTGTGGA GGCACGCAAT 
GAGGTGGCGG CTCTAGCAAT GGAGGATATG CTATCTTGGA TGCAGGAAGG TGGTCAGGTA 
GGTATCTTTG ATGCCACAAA CAGCACAAGA AACCGGAGAA ATATGCTGAT GAAAATGGCG 
GAAGGCAAGT GTAAGATCAT CTTTTTGGAA ACAATATGTA ATGACCAAAA TGTTCTCGAG 
AGAAATATGC GCTTGAAAGT TCAACGAAGT CCTGATTATG CGGAACAGAC AGATTTTGAA 
GCTGGTGTTC GAGATTTCAA AGAGAGATTG GCCTATTATG AAAAGGTATA CGAACCAGTG 
GAAGAAGGCT CATATGTTAA GATGATTGAT ATGGTCAGTG GGACTGGGGG CCAATTGCAG 
ATCAATGATA TAAGTGGTTA CTTGCCTGGC CGGATTGTTT TCTTCTTGGT GAACTGTCAT 
CTTACACCTC GTCCCATCCT GCTAACTAGA CACGGTGAAA GTTTAGATAA TGTCAGAGGA 
CGGATTGGTG GAGATTCATC TTTGAGTGAA ACCGGATCGC TTTATTCAAG GAAACTTGCC 
AGCTTTATAG AAAAGCGGCT AGCGTCTGAG AGAACTGCAT CTATATGGAC TAGCACACTC 
CAGAGGTCAA TATTAACAGC GCAGCCTATC ATTGGATTTC CAAAGATACA ATGGCGTGCA 
CTTGATGAAA TAAACGCTGG CATCTGTGAT GGGATGACGT ATGATGAAAT AAAGAAAATT 
AAACCTGAAG AATATGAATC ACGTAGCAAG GACAAGCTGA GGTATCGTTA TCCTAGAGGA 
GAATCCTACC TCGATGTCAT TCAAAGATTA GAACCTGTAA TTATTGAGCT TGAACGGCAG 
CGTGCTCCAG TTGTTGTCAT ATCTCACCAG GCTGTGCTAA GAGCACTATA TGCATATTTT 
GCGGATAAAC CACTGGAGGA ACTTCCTAAC ATTGAGATAC CACTTCATAC AATAATTGAG 
ATACAAATGG GTGTTGCTGG TGTTCAAGAG AAAAGATACA AACTCATGGA CGCAGTGAAC 
TCCACAGCAG GGATTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015630405.1
CDS	160..2337
Translation	MAAAARSSGGMSDQLFVSIKLECPRLAEMGLVPHVFGSHPVAGAWDPSKALLMEQEEAALWGLSCIVPSHRETLDFKFLLKPKDNSSHCIVEEGPDRSLVCGSNEVEIRNALFKFNDETGVVECKIFVETEILSPFDLAASWKAHQEHLQPRVRGAHDVIMNADSESRAKNGFASGLELDLEKYVVPTPNMGSDVVYAANLTENPRSLLTTGISSNNDTTKGFLHNSIKGDASPNLYASENKGADGRHVPPQEEQRAIFVDRGVGSPEFARPTNETISMSNIKFDSEAKDMPAAEGAVAAAAVADQMYGPKEDRKLAIVLVGLPARGKTFTAAKLTRYLRWLGHETKHFNVGKYRRLKHGINQTADFFRGDNKEGVEARNEVAALAMEDMLSWMQEGGQVGIFDATNSTRNRRNMLMKMAEGKCKIIFLETICNDQNVLERNMRLKVQRSPDYAEQTDFEAGVRDFKERLAYYEKVYEPVEEGSYVKMIDMVSGTGGQLQINDISGYLPGRIVFFLVNCHLTPRPILLTRHGESLDNVRGRIGGDSSLSETGSLYSRKLASFIEKRLASERTASIWTSTLQRSILTAQPIIGFPKIQWRALDEINAGICDGMTYDEIKKIKPEEYESRSKDKLRYRYPRGESYLDVIQRLEPVIIELERQRAPVVVISHQAVLRALYAYFADKPLEELPNIEIPLHTIIEIQMGVAGVQEKRYKLMDAVNSTAGI

Target ORF information:

RefSeq Version	XM_015774919.2
Organism	Oryza sativa Japonica Group(Japanese rice)
Definition	Oryza sativa Japonica Group 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase (LOC4332523), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015774919.2

ORF Insert Sequence:

ATGGCGGCGG CGGCGAGGAG CTCCGGTGGA ATGTCCGACC AGCTCTTCGT GTCCATCAAG 
CTGGAGTGCC CGCGACTCGC TGAGATGGGC CTCGTCCCGC ACGTCTTCGG CTCGCACCCA 
GTCGCCGGCG CGTGGGACCC CTCCAAGGCC CTGCTGATGG AGCAGGAGGA GGCCGCCTTG 
TGGGGGCTCA GCTGCATCGT CCCTTCTCAC CGCGAAACAC TGGATTTCAA GTTTCTATTG 
AAGCCAAAAG ACAATAGCTC ACATTGCATT GTTGAGGAGG GGCCAGACCG ATCACTAGTT 
TGTGGAAGTA ATGAGGTGGA AATTAGGAAT GCATTGTTTA AATTCAATGA TGAGACAGGT 
GTGGTTGAAT GCAAGATTTT CGTGGAGACA GAAATTTTGT CCCCATTTGA CCTAGCTGCT 
AGTTGGAAGG CTCATCAGGA GCATCTCCAG CCGAGGGTGC GAGGGGCCCA TGATGTCATT 
ATGAATGCCG ATTCTGAATC CAGGGCCAAG AATGGCTTCG CTTCTGGTTT GGAGCTTGAT 
TTAGAGAAGT ATGTAGTTCC GACACCAAAC ATGGGCTCGG ATGTTGTTTA TGCAGCTAAC 
TTGACTGAAA ATCCCCGCTC GTTATTGACT ACGGGGATTT CCTCAAACAA TGATACCACA 
AAGGGCTTTT TGCACAACTC GATCAAAGGC GATGCATCCC CGAACCTCTA TGCTAGTGAA 
AACAAGGGTG CAGATGGGAG ACATGTGCCT CCACAGGAGG AACAAAGGGC GATCTTTGTT 
GATAGAGGTG TTGGCTCACC AGAATTTGCA AGACCCACAA ATGAAACAAT TTCAATGAGC 
AACATCAAGT TTGATTCTGA AGCGAAGGAC ATGCCAGCAG CTGAAGGAGC TGTTGCAGCA 
GCAGCTGTAG CTGATCAAAT GTATGGCCCA AAGGAGGATC GCAAGTTGGC AATTGTGCTG 
GTTGGGTTGC CAGCTCGTGG TAAGACCTTT ACAGCGGCTA AGCTTACAAG GTACCTTCGA 
TGGTTAGGTC ATGAAACAAA ACACTTCAAT GTTGGAAAGT ACCGCCGACT CAAGCATGGC 
ATTAATCAGA CTGCTGATTT CTTCCGTGGA GACAACAAAG AAGGTGTGGA GGCACGCAAT 
GAGGTGGCGG CTCTAGCAAT GGAGGATATG CTATCTTGGA TGCAGGAAGG TGGTCAGGTA 
GGTATCTTTG ATGCCACAAA CAGCACAAGA AACCGGAGAA ATATGCTGAT GAAAATGGCG 
GAAGGCAAGT GTAAGATCAT CTTTTTGGAA ACAATATGTA ATGACCAAAA TGTTCTCGAG 
AGAAATATGC GCTTGAAAGT TCAACGAAGT CCTGATTATG CGGAACAGAC AGATTTTGAA 
GCTGGTGTTC GAGATTTCAA AGAGAGATTG GCCTATTATG AAAAGGTATA CGAACCAGTG 
GAAGAAGGCT CATATGTTAA GATGATTGAT ATGGTCAGTG GGACTGGGGG CCAATTGCAG 
ATCAATGATA TAAGTGGTTA CTTGCCTGGC CGGATTGTTT TCTTCTTGGT GAACTGTCAT 
CTTACACCTC GTCCCATCCT GCTAACTAGA CACGGTGAAA GTTTAGATAA TGTCAGAGGA 
CGGATTGGTG GAGATTCATC TTTGAGTGAA ACCGGATCGC TTTATTCAAG GAAACTTGCC 
AGCTTTATAG AAAAGCGGCT AGCGTCTGAG AGAACTGCAT CTATATGGAC TAGCACACTC 
CAGAGGTCAA TATTAACAGC GCAGCCTATC ATTGGATTTC CAAAGATACA ATGGCGTGCA 
CTTGATGAAA TAAACGCTGG CATCTGTGAT GGGATGACGT ATGATGAAAT AAAGAAAATT 
AAACCTGAAG AATATGAATC ACGTAGCAAG GACAAGCTGA GGTATCGTTA TCCTAGAGGA 
GAATCCTACC TCGATGTCAT TCAAAGATTA GAACCTGTAA TTATTGAGCT TGAACGGCAG 
CGTGCTCCAG TTGTTGTCAT ATCTCACCAG GCTGTGCTAA GAGCACTATA TGCATATTTT 
GCGGATAAAC CACTGGAGGA ACTTCCTAAC ATTGAGATAC CACTTCATAC AATAATTGAG 
ATACAAATGG GTGTTGCTGG TGTTCAAGAG AAAAGATACA AACTCATGGA CGCAGTGAAC 
TCCACAGCAG GGATTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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LOC4332523 cDNA ORF clone, Oryza sativa Japonica Group(Japanese rice)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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FAX

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