ptchd1 cDNA ORF clone, Oreochromis niloticus(Nile tilapia)

Gene
Clones
gRNAs

Summary

Gene Symbol	ptchd1
Entrez Gene ID	100704399
Full Name	patched domain containing 1
Gene Type	protein-coding
Organism	Oreochromis niloticus(Nile tilapia)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005449081.2	XP_005449138.1	patched domain-containing protein 1 isoform X1
XM_005449082.3	XP_005449139.1	patched domain-containing protein 1 isoform X1
XM_025910525.1	XP_025766310.1	patched domain-containing protein 1 isoform X1
XM_025910527.1	XP_025766312.1	patched domain-containing protein 1 isoform X2
XM_005449082.2	XP_005449139.1	patched domain-containing protein 1 isoform X2
XM_005449084.3	XP_005449141.1	patched domain-containing protein 1 isoform X3
XM_005449084.4	XP_005449141.1	patched domain-containing protein 1 isoform X3

Pathways from BioSystems
Gene Ontology

The following ptchd1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ptchd1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OOr174845	XM_025910527.1 Latest version!	Oreochromis niloticus patched domain containing 1 (ptchd1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OOr71678	XM_005449081.2 Latest version!	Oreochromis niloticus patched domain containing 1 (ptchd1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OOr71679	XM_005449082.3 Latest version!	Oreochromis niloticus patched domain containing 1 (ptchd1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
OOr71679	XM_025910525.1 Latest version!	Oreochromis niloticus patched domain containing 1 (ptchd1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
OOr71680	XM_005449084.4 Latest version!	Oreochromis niloticus patched domain containing 1 (ptchd1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
View Old Accession Versions >>
OOr71679	XM_005449082.2	Oreochromis niloticus patched domain containing 1 (ptchd1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
OOr71680	XM_005449084.3	Oreochromis niloticus patched domain containing 1 (ptchd1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ptchd1 ( XM_025910527.1 ) cDNA ORF clone, Oreochromis niloticus(Nile tilapia) -> XP_025766312.1 Oreochromis niloticus patched domain containing 1 (ptchd1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OOr174845
Clone ID Related Accession (Same CDS sequence)	XM_025910527.1
Accession Version	XM_025910527.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2757bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-07-25
Organism	Oreochromis niloticus(Nile tilapia)
Product	patched domain-containing protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_031974.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Oreochromis niloticus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGGACA CGCTGAGCTC TGCTAAGGAG ATTGAGTTTG TGAGAGCTCC ATCCATGCAT 
GTTTTTCTGT GTATGTGTTT ATCAAAGAAT GTTATTGTGA CCCACTTTAC ATACATGCAG 
AATTCAGCGT TGGTCCACAG AGGACTGCCA CAGTTAGAGA GCTCAGAGCT GTGCATCTTT 
TGCTGCCAAC GGGCAGCGAG TGAGAAACAG CTCCACAGAC GGATCTACCA GATGCAGGTA 
ACCGTGCCAG CAACAGGCTT CAACAGTTTC AACTACTCCT TCTCCAACCT TTGCCTTCTT 
GATGACAAGA ATGTCTGCAT CATCGATGAC ATCATCCGCG CCATGGAGGA GATCCAGTCA 
GCTCGCAACT CCAATCGCTC CGTGCCCGTC CTGAGGTACC CCATCACACA GCTGGCAGAT 
GGGCGGCAAG CGTACATTGG CCACCAGATG GGTGGTGTGC AGGGCTGGGG TCCTGGGGGG 
GCGATGCGAG TTCAGGGAAT GGGAGTGAGG GGAGAAGGTG TCCGATCTGC CCGGGCGCTG 
CAGCTCACCT ACTACCTCCA AGCCCCAGGC GGCCTGATGG ACCGGGTGGC CAGTCAGTGG 
GAAAAGGCCT TCTGTGCTGA GTTGCAGCAC TTTACAGAGT TGCACCCAAA GTTGGGCCTC 
TACCCTTCTA CTTCATCTTC TCTGAGGACA GACTTCCAGT TCTCCTCAGT GCTGGCACGT 
CGCCCCCTGT TGGCCAGCCT GGGAGTGTGT GGGGTGCTTG TCGTCCTTTG CTGCTCCATG 
AGGGACTGTG TGAGGTCCAA ACCCTGGCTG GGACTGCTAG CTCTGCTGTC CATCACTCTA 
TCAGGCCTTA CTGCTGCTGG GATACTCAAC CTGACTGGGG CCACCTACAA CTCCACGTAC 
TTGGGGATCC CGTTCGTCAT GCTTGGTCAT GGGCTGTTCG GCTCCTTCGA GATGCTATCA 
TCGTGGAGAC GAACCCGTGA GGACCAGCAT GTGAAAGAGC GTGTTGCAAG TGTCTTCGAG 
GATGTTATGT TGCGTTTCTC TGGCTCCACC ATGATCCACC TGATGACCTT GGGCCTGGCC 
GCCTCCCCGC TCACCAACAT GGAAGCCGTC CGGCTCTTCT GCCGCACCGC TGCTCTGGCC 
GTTACCATCA GCTATGTTTA CATGTTGTCC TTCTACAGTT CCTGTCTGGT GTTTACAGGC 
TACCTGGAGA CCGGGTACAG ACATGGCTGC TTCTGCCGGC GAGTCCCCAA ACCGGACCGT 
TTGGACTCTA AACCGGCTTG GTACAGGTGT TTAATGTACA CTCGTTACCA GGAGGAGACC 
CAAGTGACGA ATCCTCCACA CGGGATTGGT CACACTCACA CACCAAACCC TGACCTTACT 
CACACGCACA CGCACCCTCA CCCGCATACA ATGAACAACC CAGCTGTTCG TGGACATGCT 
CATGTTGCTA ACTCCACTCA CCCACACCCA CACCCTCACC CACACCCTCA CCCACATTCC 
ACAGCAAGCA TGGACCCTCA TCCTCAGGAC TCACACCTTT TGTTGGGCTG TGTGCGGCGT 
TGCTATGGAG ACTGGATCAC TAACACTTAT GTTAAACCCT TTGTGGTTCT GCTGTATCTG 
GTTTACATCT CATTTGGATT GATGGGATTC CTACAGGTGA CCCAGGGTTC TGACCCCAGC 
GCACTGGTTG CAATGGACAC CGCAACAGTA TTGTACACCC GAGCCCAGCA GCGTTACTTC 
AGCTCATACT CTCCTGTGAT TGGATTCTAC ATTTATGAAA GTGCCCCTTA CTGGAATGCC 
TCAGTGCAGC GGGATCTGCT GGAATATGCC AAAGGCTTCC AGAGAATAAG CTGGCTGGAA 
GCTTACCTGA ACTACCTGTC GGATCGCAAC CAGTCCACCA GCCAGCCGCG GGAAAATTTC 
ACCCGCACGC TACGCCACGC CTTTCTCCGT GAGCCGCAGT TTGCTCACTT TGCGGATGAC 
ATTATATTTG CAGAGCGCGG CCAGGGCGAA GAGCCTGATG TGGCAGCTTC TCGCATATTC 
TTGGTTGCCA AAACAACGGA GAACAAGCGC GAGGAGATGT CAGTGCTGCT GGACACGCTG 
CGACGCCTGT CACTGACCTC ACGAGTCCGC TTCTTAATCT TTAACCCTTC TTTCGTCTAC 
CTGGACCGCT ATGCTGCGGC GGTCAGCTCC CCACTTAGAC ACTCACTGCT GGCGGTGCTC 
TTCCTGTTGG GTCTCTCCTC TCTGGCTGTT GTGGAGCCCC TGGTGTCAGT GTGGCTGGGC 
CTCACCCTGC TCTCTGTCCA GTTTGGCGTC CTGGGCTTCA TGACCTTATG GGGTGTAGAG 
CTGGATTGCA TGTCTGTGCT ATGTTTGATC TCAGCTTTGG GGCACTCAGC AGACTGCAGC 
GGCCCTCTCC TCTGTGGCTT TGCTGCAGGT CGGGGTGAAA GCAGGACTCG CTGGGTGAGA 
GTGGCACTGG AGAGACACGG GGTTCCCTCT CTGCAGACTC TCATCTGTTA CGGCGCTGCC 
CTGGTACCCG TTGGCTCCAT ACGCTCCAAC CTCACACGCA CACTATTTCG CTGCCTCACC 
CTAACAGCTG GTTGCTCGGC CCTGCACACG CTGGCATTCC TGCCCACACT CCTCACCTTC 
CTGCCCCCCT CCAAGAGCCG GGGCCATGGA CCTGGAGGGG AAGGACAGAG ACAGGAGGTG 
GAGTGTGTTG AAATGAATGA CAGCACCAGA GTTGTCGACC AGATCACCAC CGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025766312.1
CDS	19..2775
Translation	MLDTLSSAKEIEFVRAPSMHVFLCMCLSKNVIVTHFTYMQNSALVHRGLPQLESSELCIFCCQRAASEKQLHRRIYQMQVTVPATGFNSFNYSFSNLCLLDDKNVCIIDDIIRAMEEIQSARNSNRSVPVLRYPITQLADGRQAYIGHQMGGVQGWGPGGAMRVQGMGVRGEGVRSARALQLTYYLQAPGGLMDRVASQWEKAFCAELQHFTELHPKLGLYPSTSSSLRTDFQFSSVLARRPLLASLGVCGVLVVLCCSMRDCVRSKPWLGLLALLSITLSGLTAAGILNLTGATYNSTYLGIPFVMLGHGLFGSFEMLSSWRRTREDQHVKERVASVFEDVMLRFSGSTMIHLMTLGLAASPLTNMEAVRLFCRTAALAVTISYVYMLSFYSSCLVFTGYLETGYRHGCFCRRVPKPDRLDSKPAWYRCLMYTRYQEETQVTNPPHGIGHTHTPNPDLTHTHTHPHPHTMNNPAVRGHAHVANSTHPHPHPHPHPHPHSTASMDPHPQDSHLLLGCVRRCYGDWITNTYVKPFVVLLYLVYISFGLMGFLQVTQGSDPSALVAMDTATVLYTRAQQRYFSSYSPVIGFYIYESAPYWNASVQRDLLEYAKGFQRISWLEAYLNYLSDRNQSTSQPRENFTRTLRHAFLREPQFAHFADDIIFAERGQGEEPDVAASRIFLVAKTTENKREEMSVLLDTLRRLSLTSRVRFLIFNPSFVYLDRYAAAVSSPLRHSLLAVLFLLGLSSLAVVEPLVSVWLGLTLLSVQFGVLGFMTLWGVELDCMSVLCLISALGHSADCSGPLLCGFAAGRGESRTRWVRVALERHGVPSLQTLICYGAALVPVGSIRSNLTRTLFRCLTLTAGCSALHTLAFLPTLLTFLPPSKSRGHGPGGEGQRQEVECVEMNDSTRVVDQITTV

Target ORF information:

RefSeq Version	XM_025910527.1
Organism	Oreochromis niloticus(Nile tilapia)
Definition	Oreochromis niloticus patched domain containing 1 (ptchd1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025910527.1

ORF Insert Sequence:

ATGCTGGACA CGCTGAGCTC TGCTAAGGAG ATTGAGTTTG TGAGAGCTCC ATCCATGCAT 
GTTTTTCTGT GTATGTGTTT ATCAAAGAAT GTTATTGTGA CCCACTTTAC ATACATGCAG 
AATTCAGCGT TGGTCCACAG AGGACTGCCA CAGTTAGAGA GCTCAGAGCT GTGCATCTTT 
TGCTGCCAAC GGGCAGCGAG TGAGAAACAG CTCCACAGAC GGATCTACCA GATGCAGGTA 
ACCGTGCCAG CAACAGGCTT CAACAGTTTC AACTACTCCT TCTCCAACCT TTGCCTTCTT 
GATGACAAGA ATGTCTGCAT CATCGATGAC ATCATCCGCG CCATGGAGGA GATCCAGTCA 
GCTCGCAACT CCAATCGCTC CGTGCCCGTC CTGAGGTACC CCATCACACA GCTGGCAGAT 
GGGCGGCAAG CGTACATTGG CCACCAGATG GGTGGTGTGC AGGGCTGGGG TCCTGGGGGG 
GCGATGCGAG TTCAGGGAAT GGGAGTGAGG GGAGAAGGTG TCCGATCTGC CCGGGCGCTG 
CAGCTCACCT ACTACCTCCA AGCCCCAGGC GGCCTGATGG ACCGGGTGGC CAGTCAGTGG 
GAAAAGGCCT TCTGTGCTGA GTTGCAGCAC TTTACAGAGT TGCACCCAAA GTTGGGCCTC 
TACCCTTCTA CTTCATCTTC TCTGAGGACA GACTTCCAGT TCTCCTCAGT GCTGGCACGT 
CGCCCCCTGT TGGCCAGCCT GGGAGTGTGT GGGGTGCTTG TCGTCCTTTG CTGCTCCATG 
AGGGACTGTG TGAGGTCCAA ACCCTGGCTG GGACTGCTAG CTCTGCTGTC CATCACTCTA 
TCAGGCCTTA CTGCTGCTGG GATACTCAAC CTGACTGGGG CCACCTACAA CTCCACGTAC 
TTGGGGATCC CGTTCGTCAT GCTTGGTCAT GGGCTGTTCG GCTCCTTCGA GATGCTATCA 
TCGTGGAGAC GAACCCGTGA GGACCAGCAT GTGAAAGAGC GTGTTGCAAG TGTCTTCGAG 
GATGTTATGT TGCGTTTCTC TGGCTCCACC ATGATCCACC TGATGACCTT GGGCCTGGCC 
GCCTCCCCGC TCACCAACAT GGAAGCCGTC CGGCTCTTCT GCCGCACCGC TGCTCTGGCC 
GTTACCATCA GCTATGTTTA CATGTTGTCC TTCTACAGTT CCTGTCTGGT GTTTACAGGC 
TACCTGGAGA CCGGGTACAG ACATGGCTGC TTCTGCCGGC GAGTCCCCAA ACCGGACCGT 
TTGGACTCTA AACCGGCTTG GTACAGGTGT TTAATGTACA CTCGTTACCA GGAGGAGACC 
CAAGTGACGA ATCCTCCACA CGGGATTGGT CACACTCACA CACCAAACCC TGACCTTACT 
CACACGCACA CGCACCCTCA CCCGCATACA ATGAACAACC CAGCTGTTCG TGGACATGCT 
CATGTTGCTA ACTCCACTCA CCCACACCCA CACCCTCACC CACACCCTCA CCCACATTCC 
ACAGCAAGCA TGGACCCTCA TCCTCAGGAC TCACACCTTT TGTTGGGCTG TGTGCGGCGT 
TGCTATGGAG ACTGGATCAC TAACACTTAT GTTAAACCCT TTGTGGTTCT GCTGTATCTG 
GTTTACATCT CATTTGGATT GATGGGATTC CTACAGGTGA CCCAGGGTTC TGACCCCAGC 
GCACTGGTTG CAATGGACAC CGCAACAGTA TTGTACACCC GAGCCCAGCA GCGTTACTTC 
AGCTCATACT CTCCTGTGAT TGGATTCTAC ATTTATGAAA GTGCCCCTTA CTGGAATGCC 
TCAGTGCAGC GGGATCTGCT GGAATATGCC AAAGGCTTCC AGAGAATAAG CTGGCTGGAA 
GCTTACCTGA ACTACCTGTC GGATCGCAAC CAGTCCACCA GCCAGCCGCG GGAAAATTTC 
ACCCGCACGC TACGCCACGC CTTTCTCCGT GAGCCGCAGT TTGCTCACTT TGCGGATGAC 
ATTATATTTG CAGAGCGCGG CCAGGGCGAA GAGCCTGATG TGGCAGCTTC TCGCATATTC 
TTGGTTGCCA AAACAACGGA GAACAAGCGC GAGGAGATGT CAGTGCTGCT GGACACGCTG 
CGACGCCTGT CACTGACCTC ACGAGTCCGC TTCTTAATCT TTAACCCTTC TTTCGTCTAC 
CTGGACCGCT ATGCTGCGGC GGTCAGCTCC CCACTTAGAC ACTCACTGCT GGCGGTGCTC 
TTCCTGTTGG GTCTCTCCTC TCTGGCTGTT GTGGAGCCCC TGGTGTCAGT GTGGCTGGGC 
CTCACCCTGC TCTCTGTCCA GTTTGGCGTC CTGGGCTTCA TGACCTTATG GGGTGTAGAG 
CTGGATTGCA TGTCTGTGCT ATGTTTGATC TCAGCTTTGG GGCACTCAGC AGACTGCAGC 
GGCCCTCTCC TCTGTGGCTT TGCTGCAGGT CGGGGTGAAA GCAGGACTCG CTGGGTGAGA 
GTGGCACTGG AGAGACACGG GGTTCCCTCT CTGCAGACTC TCATCTGTTA CGGCGCTGCC 
CTGGTACCCG TTGGCTCCAT ACGCTCCAAC CTCACACGCA CACTATTTCG CTGCCTCACC 
CTAACAGCTG GTTGCTCGGC CCTGCACACG CTGGCATTCC TGCCCACACT CCTCACCTTC 
CTGCCCCCCT CCAAGAGCCG GGGCCATGGA CCTGGAGGGG AAGGACAGAG ACAGGAGGTG 
GAGTGTGTTG AAATGAATGA CAGCACCAGA GTTGTCGACC AGATCACCAC CGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ptchd1 ( XM_005449081.2 ) cDNA ORF clone, Oreochromis niloticus(Nile tilapia) -> XP_005449138.1 Oreochromis niloticus patched domain containing 1 (ptchd1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OOr71678
Clone ID Related Accession (Same CDS sequence)	XM_005449081.2
Accession Version	XM_005449081.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3051bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-12-04
Organism	Oreochromis niloticus(Nile tilapia)
Product	patched domain-containing protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_031974.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 5, 2016 this sequence version replaced XM_005449081.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oreochromis niloticus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCGGCA GTGCTCCGGG GCTTTCCGGT CGGTGGGAGA GGCTCGAGCC GCGGCCGCGC 
GCTAGGATGC TGCGCCAGGT GTTGCACGCG GGCCTGAGGA CCTCGTTCCA CGCGCTGGGT 
CGGTTCGTGG CGAGCCACCC GGTCTTCTTC GCCTCGGCAC CCGTGCTCCT CTCCATCTTG 
CTGGGTTCCA GCTTTAGCCG GTACCGCGTG GAAGAGGACG TGGAGAGCCT GCTCGCGCCC 
AAGCACAGCC TGGCCAAGAT AGAGGGCAAC CTCGTGGACA GCCTGTTCCC AGTGAACCGC 
TCCAAGCACG CGCTCTACTC TGACCTGCAG ACGCCGGGCC GCTATGGGCG CGTGATCATC 
ACCACCCGAA AGGGAAGCGT GCTGGATCCG GTTCATCTGG ACGCCGTACT AAAGAATTCA 
GCGTTGGTCC ACAGAGGACT GCCACAGTTA GAGAGCTCAG AGCTGTGCAT CTTTTGCTGC 
CAACGGGCAG CGAGTGAGAA ACAGCTCCAC AGACGGATCT ACCAGATGCA GGTAACCGTG 
CCAGCAACAG GCTTCAACAG TTTCAACTAC TCCTTCTCCA ACCTTTGCCT TCTTGATGAC 
AAGAATGTCT GCATCATCGA TGACATCATC CGCGCCATGG AGGAGATCCA GTCAGCTCGC 
AACTCCAATC GCTCCGTGCC CGTCCTGAGG TACCCCATCA CACAGCTGGC AGATGGGCGG 
CAAGCGTACA TTGGCCACCA GATGGGTGGT GTGCAGGGCT GGGGTCCTGG GGGGGCGATG 
CGAGTTCAGG GAATGGGAGT GAGGGGAGAA GGTGTCCGAT CTGCCCGGGC GCTGCAGCTC 
ACCTACTACC TCCAAGCCCC AGGCGGCCTG ATGGACCGGG TGGCCAGTCA GTGGGAAAAG 
GCCTTCTGTG CTGAGTTGCA GCACTTTACA GAGTTGCACC CAAAGTTGGG CCTCTACCCT 
TCTACTTCAT CTTCTCTGAG GACAGACTTC CAGTTCTCCT CAGTGCTGGC ACGTCGCCCC 
CTGTTGGCCA GCCTGGGAGT GTGTGGGGTG CTTGTCGTCC TTTGCTGCTC CATGAGGGAC 
TGTGTGAGGT CCAAACCCTG GCTGGGACTG CTAGCTCTGC TGTCCATCAC TCTATCAGGC 
CTTACTGCTG CTGGGATACT CAACCTGACT GGGGCCACCT ACAACTCCAC GTACTTGGGG 
ATCCCGTTCG TCATGCTTGG TCATGGGCTG TTCGGCTCCT TCGAGATGCT ATCATCGTGG 
AGACGAACCC GTGAGGACCA GCATGTGAAA GAGCGTGTTG CAAGTGTCTT CGAGGATGTT 
ATGTTGCGTT TCTCTGGCTC CACCATGATC CACCTGATGA CCTTGGGCCT GGCCGCCTCC 
CCGCTCACCA ACATGGAAGC CGTCCGGCTC TTCTGCCGCA CCGCTGCTCT GGCCGTTACC 
ATCAGCTATG TTTACATGTT GTCCTTCTAC AGTTCCTGTC TGGTGTTTAC AGGCTACCTG 
GAGACCGGGT ACAGACATGG CTGCTTCTGC CGGCGAGTCC CCAAACCGGA CCGTTTGGAC 
TCTAAACCGG CTTGGTACAG GTGTTTAATG TACACTCGTT ACCAGGAGGA GACCCAAGTG 
ACGAATCCTC CACACGGGAT TGGTCACACT CACACACCAA ACCCTGACCT TACTCACACG 
CACACGCACC CTCACCCGCA TACAATGAAC AACCCAGCTG TTCGTGGACA TGCTCATGTT 
GCTAACTCCA CTCACCCACA CCCACACCCT CACCCACACC CTCACCCACA TTCCACAGCA 
AGCATGGACC CTCATCCTCA GGACTCACAC CTTTTGTTGG GCTGTGTGCG GCGTTGCTAT 
GGAGACTGGA TCACTAACAC TTATGTTAAA CCCTTTGTGG TTCTGCTGTA TCTGGTTTAC 
ATCTCATTTG GATTGATGGG ATTCCTACAG GTGACCCAGG GTTCTGACCC CAGCGCACTG 
GTTGCAATGG ACACCGCAAC AGTATTGTAC ACCCGAGCCC AGCAGCGTTA CTTCAGCTCA 
TACTCTCCTG TGATTGGATT CTACATTTAT GAAAGTGCCC CTTACTGGAA TGCCTCAGTG 
CAGCGGGATC TGCTGGAATA TGCCAAAGGC TTCCAGAGAA TAAGCTGGCT GGAAGCTTAC 
CTGAACTACC TGTCGGATCG CAACCAGTCC ACCAGCCAGC CGCGGGAAAA TTTCACCCGC 
ACGCTACGCC ACGCCTTTCT CCGTGAGCCG CAGTTTGCTC ACTTTGCGGA TGACATTATA 
TTTGCAGAGC GCGGCCAGGG CGAAGAGCCT GATGTGGCAG CTTCTCGCAT ATTCTTGGTT 
GCCAAAACAA CGGAGAACAA GCGCGAGGAG ATGTCAGTGC TGCTGGACAC GCTGCGACGC 
CTGTCACTGA CCTCACGAGT CCGCTTCTTA ATCTTTAACC CTTCTTTCGT CTACCTGGAC 
CGCTATGCTG CGGCGGTCAG CTCCCCACTT AGACACTCAC TGCTGGCGGT GCTCTTCCTG 
TTGGGTCTCT CCTCTCTGGC TGTTGTGGAG CCCCTGGTGT CAGTGTGGCT GGGCCTCACC 
CTGCTCTCTG TCCAGTTTGG CGTCCTGGGC TTCATGACCT TATGGGGTGT AGAGCTGGAT 
TGCATGTCTG TGCTATGTTT GATCTCAGCT TTGGGGCACT CAGCAGACTG CAGCGGCCCT 
CTCCTCTGTG GCTTTGCTGC AGGTCGGGGT GAAAGCAGGA CTCGCTGGGT GAGAGTGGCA 
CTGGAGAGAC ACGGGGTTCC CTCTCTGCAG ACTCTCATCT GTTACGGCGC TGCCCTGGTA 
CCCGTTGGCT CCATACGCTC CAACCTCACA CGCACACTAT TTCGCTGCCT CACCCTAACA 
GCTGGTTGCT CGGCCCTGCA CACGCTGGCA TTCCTGCCCA CACTCCTCAC CTTCCTGCCC 
CCCTCCAAGA GCCGGGGCCA TGGACCTGGA GGGGAAGGAC AGAGACAGGA GGTGGAGTGT 
GTTGAAATGA ATGACAGCAC CAGAGTTGTC GACCAGATCA CCACCGTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005449138.1
CDS	960..4010
Translation	MLGSAPGLSGRWERLEPRPRARMLRQVLHAGLRTSFHALGRFVASHPVFFASAPVLLSILLGSSFSRYRVEEDVESLLAPKHSLAKIEGNLVDSLFPVNRSKHALYSDLQTPGRYGRVIITTRKGSVLDPVHLDAVLKNSALVHRGLPQLESSELCIFCCQRAASEKQLHRRIYQMQVTVPATGFNSFNYSFSNLCLLDDKNVCIIDDIIRAMEEIQSARNSNRSVPVLRYPITQLADGRQAYIGHQMGGVQGWGPGGAMRVQGMGVRGEGVRSARALQLTYYLQAPGGLMDRVASQWEKAFCAELQHFTELHPKLGLYPSTSSSLRTDFQFSSVLARRPLLASLGVCGVLVVLCCSMRDCVRSKPWLGLLALLSITLSGLTAAGILNLTGATYNSTYLGIPFVMLGHGLFGSFEMLSSWRRTREDQHVKERVASVFEDVMLRFSGSTMIHLMTLGLAASPLTNMEAVRLFCRTAALAVTISYVYMLSFYSSCLVFTGYLETGYRHGCFCRRVPKPDRLDSKPAWYRCLMYTRYQEETQVTNPPHGIGHTHTPNPDLTHTHTHPHPHTMNNPAVRGHAHVANSTHPHPHPHPHPHPHSTASMDPHPQDSHLLLGCVRRCYGDWITNTYVKPFVVLLYLVYISFGLMGFLQVTQGSDPSALVAMDTATVLYTRAQQRYFSSYSPVIGFYIYESAPYWNASVQRDLLEYAKGFQRISWLEAYLNYLSDRNQSTSQPRENFTRTLRHAFLREPQFAHFADDIIFAERGQGEEPDVAASRIFLVAKTTENKREEMSVLLDTLRRLSLTSRVRFLIFNPSFVYLDRYAAAVSSPLRHSLLAVLFLLGLSSLAVVEPLVSVWLGLTLLSVQFGVLGFMTLWGVELDCMSVLCLISALGHSADCSGPLLCGFAAGRGESRTRWVRVALERHGVPSLQTLICYGAALVPVGSIRSNLTRTLFRCLTLTAGCSALHTLAFLPTLLTFLPPSKSRGHGPGGEGQRQEVECVEMNDSTRVVDQITTV

Target ORF information:

RefSeq Version	XM_005449081.2
Organism	Oreochromis niloticus(Nile tilapia)
Definition	Oreochromis niloticus patched domain containing 1 (ptchd1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005449081.2

ORF Insert Sequence:

ATGCTCGGCA GTGCTCCGGG GCTTTCCGGT CGGTGGGAGA GGCTCGAGCC GCGGCCGCGC 
GCTAGGATGC TGCGCCAGGT GTTGCACGCG GGCCTGAGGA CCTCGTTCCA CGCGCTGGGT 
CGGTTCGTGG CGAGCCACCC GGTCTTCTTC GCCTCGGCAC CCGTGCTCCT CTCCATCTTG 
CTGGGTTCCA GCTTTAGCCG GTACCGCGTG GAAGAGGACG TGGAGAGCCT GCTCGCGCCC 
AAGCACAGCC TGGCCAAGAT AGAGGGCAAC CTCGTGGACA GCCTGTTCCC AGTGAACCGC 
TCCAAGCACG CGCTCTACTC TGACCTGCAG ACGCCGGGCC GCTATGGGCG CGTGATCATC 
ACCACCCGAA AGGGAAGCGT GCTGGATCCG GTTCATCTGG ACGCCGTACT AAAGAATTCA 
GCGTTGGTCC ACAGAGGACT GCCACAGTTA GAGAGCTCAG AGCTGTGCAT CTTTTGCTGC 
CAACGGGCAG CGAGTGAGAA ACAGCTCCAC AGACGGATCT ACCAGATGCA GGTAACCGTG 
CCAGCAACAG GCTTCAACAG TTTCAACTAC TCCTTCTCCA ACCTTTGCCT TCTTGATGAC 
AAGAATGTCT GCATCATCGA TGACATCATC CGCGCCATGG AGGAGATCCA GTCAGCTCGC 
AACTCCAATC GCTCCGTGCC CGTCCTGAGG TACCCCATCA CACAGCTGGC AGATGGGCGG 
CAAGCGTACA TTGGCCACCA GATGGGTGGT GTGCAGGGCT GGGGTCCTGG GGGGGCGATG 
CGAGTTCAGG GAATGGGAGT GAGGGGAGAA GGTGTCCGAT CTGCCCGGGC GCTGCAGCTC 
ACCTACTACC TCCAAGCCCC AGGCGGCCTG ATGGACCGGG TGGCCAGTCA GTGGGAAAAG 
GCCTTCTGTG CTGAGTTGCA GCACTTTACA GAGTTGCACC CAAAGTTGGG CCTCTACCCT 
TCTACTTCAT CTTCTCTGAG GACAGACTTC CAGTTCTCCT CAGTGCTGGC ACGTCGCCCC 
CTGTTGGCCA GCCTGGGAGT GTGTGGGGTG CTTGTCGTCC TTTGCTGCTC CATGAGGGAC 
TGTGTGAGGT CCAAACCCTG GCTGGGACTG CTAGCTCTGC TGTCCATCAC TCTATCAGGC 
CTTACTGCTG CTGGGATACT CAACCTGACT GGGGCCACCT ACAACTCCAC GTACTTGGGG 
ATCCCGTTCG TCATGCTTGG TCATGGGCTG TTCGGCTCCT TCGAGATGCT ATCATCGTGG 
AGACGAACCC GTGAGGACCA GCATGTGAAA GAGCGTGTTG CAAGTGTCTT CGAGGATGTT 
ATGTTGCGTT TCTCTGGCTC CACCATGATC CACCTGATGA CCTTGGGCCT GGCCGCCTCC 
CCGCTCACCA ACATGGAAGC CGTCCGGCTC TTCTGCCGCA CCGCTGCTCT GGCCGTTACC 
ATCAGCTATG TTTACATGTT GTCCTTCTAC AGTTCCTGTC TGGTGTTTAC AGGCTACCTG 
GAGACCGGGT ACAGACATGG CTGCTTCTGC CGGCGAGTCC CCAAACCGGA CCGTTTGGAC 
TCTAAACCGG CTTGGTACAG GTGTTTAATG TACACTCGTT ACCAGGAGGA GACCCAAGTG 
ACGAATCCTC CACACGGGAT TGGTCACACT CACACACCAA ACCCTGACCT TACTCACACG 
CACACGCACC CTCACCCGCA TACAATGAAC AACCCAGCTG TTCGTGGACA TGCTCATGTT 
GCTAACTCCA CTCACCCACA CCCACACCCT CACCCACACC CTCACCCACA TTCCACAGCA 
AGCATGGACC CTCATCCTCA GGACTCACAC CTTTTGTTGG GCTGTGTGCG GCGTTGCTAT 
GGAGACTGGA TCACTAACAC TTATGTTAAA CCCTTTGTGG TTCTGCTGTA TCTGGTTTAC 
ATCTCATTTG GATTGATGGG ATTCCTACAG GTGACCCAGG GTTCTGACCC CAGCGCACTG 
GTTGCAATGG ACACCGCAAC AGTATTGTAC ACCCGAGCCC AGCAGCGTTA CTTCAGCTCA 
TACTCTCCTG TGATTGGATT CTACATTTAT GAAAGTGCCC CTTACTGGAA TGCCTCAGTG 
CAGCGGGATC TGCTGGAATA TGCCAAAGGC TTCCAGAGAA TAAGCTGGCT GGAAGCTTAC 
CTGAACTACC TGTCGGATCG CAACCAGTCC ACCAGCCAGC CGCGGGAAAA TTTCACCCGC 
ACGCTACGCC ACGCCTTTCT CCGTGAGCCG CAGTTTGCTC ACTTTGCGGA TGACATTATA 
TTTGCAGAGC GCGGCCAGGG CGAAGAGCCT GATGTGGCAG CTTCTCGCAT ATTCTTGGTT 
GCCAAAACAA CGGAGAACAA GCGCGAGGAG ATGTCAGTGC TGCTGGACAC GCTGCGACGC 
CTGTCACTGA CCTCACGAGT CCGCTTCTTA ATCTTTAACC CTTCTTTCGT CTACCTGGAC 
CGCTATGCTG CGGCGGTCAG CTCCCCACTT AGACACTCAC TGCTGGCGGT GCTCTTCCTG 
TTGGGTCTCT CCTCTCTGGC TGTTGTGGAG CCCCTGGTGT CAGTGTGGCT GGGCCTCACC 
CTGCTCTCTG TCCAGTTTGG CGTCCTGGGC TTCATGACCT TATGGGGTGT AGAGCTGGAT 
TGCATGTCTG TGCTATGTTT GATCTCAGCT TTGGGGCACT CAGCAGACTG CAGCGGCCCT 
CTCCTCTGTG GCTTTGCTGC AGGTCGGGGT GAAAGCAGGA CTCGCTGGGT GAGAGTGGCA 
CTGGAGAGAC ACGGGGTTCC CTCTCTGCAG ACTCTCATCT GTTACGGCGC TGCCCTGGTA 
CCCGTTGGCT CCATACGCTC CAACCTCACA CGCACACTAT TTCGCTGCCT CACCCTAACA 
GCTGGTTGCT CGGCCCTGCA CACGCTGGCA TTCCTGCCCA CACTCCTCAC CTTCCTGCCC 
CCCTCCAAGA GCCGGGGCCA TGGACCTGGA GGGGAAGGAC AGAGACAGGA GGTGGAGTGT 
GTTGAAATGA ATGACAGCAC CAGAGTTGTC GACCAGATCA CCACCGTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ptchd1 ( XM_005449082.3 ) cDNA ORF clone, Oreochromis niloticus(Nile tilapia) -> XP_005449139.1 Oreochromis niloticus patched domain containing 1 (ptchd1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OOr71679
Clone ID Related Accession (Same CDS sequence)	XM_005449082.3 , XM_025910525.1 , XM_005449082.2
Accession Version	XM_005449082.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2961bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-07-25
Organism	Oreochromis niloticus(Nile tilapia)
Product	patched domain-containing protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_031974.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2018 this sequence version replaced XM_005449082.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Oreochromis niloticus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCGGCA GTGCTCCGGG GCTTTCCGGT CGGTGGGAGA GGCTCGAGCC GCGGCCGCGC 
GCTAGGATGC TGCGCCAGGT GTTGCACGCG GGCCTGAGGA CCTCGTTCCA CGCGCTGGGT 
CGGTTCGTGG CGAGCCACCC GGTCTTCTTC GCCTCGGCAC CCGTGCTCCT CTCCATCTTG 
CTGGGTTCCA GCTTTAGCCG GTACCGCGTG GAAGAGGACG TGGAGAGCCT GCTCGCGCCC 
AAGCACAGCC TGGCCAAGAT AGAGGGCAAC CTCGTGGACA GCCTGTTCCC AGTGAACCGC 
TCCAAGCACG CGCTCTACTC TGACCTGCAG ACGCCGGGCC GCTATGGGCG CGTGATCATC 
ACCACCCGAA AGGGAAGCGT GCTGGATCCG GTTCATCTGG ACGCCGTACT AAAGCTCCAC 
AGACGGATCT ACCAGATGCA GGTAACCGTG CCAGCAACAG GCTTCAACAG TTTCAACTAC 
TCCTTCTCCA ACCTTTGCCT TCTTGATGAC AAGAATGTCT GCATCATCGA TGACATCATC 
CGCGCCATGG AGGAGATCCA GTCAGCTCGC AACTCCAATC GCTCCGTGCC CGTCCTGAGG 
TACCCCATCA CACAGCTGGC AGATGGGCGG CAAGCGTACA TTGGCCACCA GATGGGTGGT 
GTGCAGGGCT GGGGTCCTGG GGGGGCGATG CGAGTTCAGG GAATGGGAGT GAGGGGAGAA 
GGTGTCCGAT CTGCCCGGGC GCTGCAGCTC ACCTACTACC TCCAAGCCCC AGGCGGCCTG 
ATGGACCGGG TGGCCAGTCA GTGGGAAAAG GCCTTCTGTG CTGAGTTGCA GCACTTTACA 
GAGTTGCACC CAAAGTTGGG CCTCTACCCT TCTACTTCAT CTTCTCTGAG GACAGACTTC 
CAGTTCTCCT CAGTGCTGGC ACGTCGCCCC CTGTTGGCCA GCCTGGGAGT GTGTGGGGTG 
CTTGTCGTCC TTTGCTGCTC CATGAGGGAC TGTGTGAGGT CCAAACCCTG GCTGGGACTG 
CTAGCTCTGC TGTCCATCAC TCTATCAGGC CTTACTGCTG CTGGGATACT CAACCTGACT 
GGGGCCACCT ACAACTCCAC GTACTTGGGG ATCCCGTTCG TCATGCTTGG TCATGGGCTG 
TTCGGCTCCT TCGAGATGCT ATCATCGTGG AGACGAACCC GTGAGGACCA GCATGTGAAA 
GAGCGTGTTG CAAGTGTCTT CGAGGATGTT ATGTTGCGTT TCTCTGGCTC CACCATGATC 
CACCTGATGA CCTTGGGCCT GGCCGCCTCC CCGCTCACCA ACATGGAAGC CGTCCGGCTC 
TTCTGCCGCA CCGCTGCTCT GGCCGTTACC ATCAGCTATG TTTACATGTT GTCCTTCTAC 
AGTTCCTGTC TGGTGTTTAC AGGCTACCTG GAGACCGGGT ACAGACATGG CTGCTTCTGC 
CGGCGAGTCC CCAAACCGGA CCGTTTGGAC TCTAAACCGG CTTGGTACAG GTGTTTAATG 
TACACTCGTT ACCAGGAGGA GACCCAAGTG ACGAATCCTC CACACGGGAT TGGTCACACT 
CACACACCAA ACCCTGACCT TACTCACACG CACACGCACC CTCACCCGCA TACAATGAAC 
AACCCAGCTG TTCGTGGACA TGCTCATGTT GCTAACTCCA CTCACCCACA CCCACACCCT 
CACCCACACC CTCACCCACA TTCCACAGCA AGCATGGACC CTCATCCTCA GGACTCACAC 
CTTTTGTTGG GCTGTGTGCG GCGTTGCTAT GGAGACTGGA TCACTAACAC TTATGTTAAA 
CCCTTTGTGG TTCTGCTGTA TCTGGTTTAC ATCTCATTTG GATTGATGGG ATTCCTACAG 
GTGACCCAGG GTTCTGACCC CAGCGCACTG GTTGCAATGG ACACCGCAAC AGTATTGTAC 
ACCCGAGCCC AGCAGCGTTA CTTCAGCTCA TACTCTCCTG TGATTGGATT CTACATTTAT 
GAAAGTGCCC CTTACTGGAA TGCCTCAGTG CAGCGGGATC TGCTGGAATA TGCCAAAGGC 
TTCCAGAGAA TAAGCTGGCT GGAAGCTTAC CTGAACTACC TGTCGGATCG CAACCAGTCC 
ACCAGCCAGC CGCGGGAAAA TTTCACCCGC ACGCTACGCC ACGCCTTTCT CCGTGAGCCG 
CAGTTTGCTC ACTTTGCGGA TGACATTATA TTTGCAGAGC GCGGCCAGGG CGAAGAGCCT 
GATGTGGCAG CTTCTCGCAT ATTCTTGGTT GCCAAAACAA CGGAGAACAA GCGCGAGGAG 
ATGTCAGTGC TGCTGGACAC GCTGCGACGC CTGTCACTGA CCTCACGAGT CCGCTTCTTA 
ATCTTTAACC CTTCTTTCGT CTACCTGGAC CGCTATGCTG CGGCGGTCAG CTCCCCACTT 
AGACACTCAC TGCTGGCGGT GCTCTTCCTG TTGGGTCTCT CCTCTCTGGC TGTTGTGGAG 
CCCCTGGTGT CAGTGTGGCT GGGCCTCACC CTGCTCTCTG TCCAGTTTGG CGTCCTGGGC 
TTCATGACCT TATGGGGTGT AGAGCTGGAT TGCATGTCTG TGCTATGTTT GATCTCAGCT 
TTGGGGCACT CAGCAGACTG CAGCGGCCCT CTCCTCTGTG GCTTTGCTGC AGGTCGGGGT 
GAAAGCAGGA CTCGCTGGGT GAGAGTGGCA CTGGAGAGAC ACGGGGTTCC CTCTCTGCAG 
ACTCTCATCT GTTACGGCGC TGCCCTGGTA CCCGTTGGCT CCATACGCTC CAACCTCACA 
CGCACACTAT TTCGCTGCCT CACCCTAACA GCTGGTTGCT CGGCCCTGCA CACGCTGGCA 
TTCCTGCCCA CACTCCTCAC CTTCCTGCCC CCCTCCAAGA GCCGGGGCCA TGGACCTGGA 
GGGGAAGGAC AGAGACAGGA GGTGGAGTGT GTTGAAATGA ATGACAGCAC CAGAGTTGTC 
GACCAGATCA CCACCGTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005449139.1
CDS	1670..4630
Translation	MLGSAPGLSGRWERLEPRPRARMLRQVLHAGLRTSFHALGRFVASHPVFFASAPVLLSILLGSSFSRYRVEEDVESLLAPKHSLAKIEGNLVDSLFPVNRSKHALYSDLQTPGRYGRVIITTRKGSVLDPVHLDAVLKLHRRIYQMQVTVPATGFNSFNYSFSNLCLLDDKNVCIIDDIIRAMEEIQSARNSNRSVPVLRYPITQLADGRQAYIGHQMGGVQGWGPGGAMRVQGMGVRGEGVRSARALQLTYYLQAPGGLMDRVASQWEKAFCAELQHFTELHPKLGLYPSTSSSLRTDFQFSSVLARRPLLASLGVCGVLVVLCCSMRDCVRSKPWLGLLALLSITLSGLTAAGILNLTGATYNSTYLGIPFVMLGHGLFGSFEMLSSWRRTREDQHVKERVASVFEDVMLRFSGSTMIHLMTLGLAASPLTNMEAVRLFCRTAALAVTISYVYMLSFYSSCLVFTGYLETGYRHGCFCRRVPKPDRLDSKPAWYRCLMYTRYQEETQVTNPPHGIGHTHTPNPDLTHTHTHPHPHTMNNPAVRGHAHVANSTHPHPHPHPHPHPHSTASMDPHPQDSHLLLGCVRRCYGDWITNTYVKPFVVLLYLVYISFGLMGFLQVTQGSDPSALVAMDTATVLYTRAQQRYFSSYSPVIGFYIYESAPYWNASVQRDLLEYAKGFQRISWLEAYLNYLSDRNQSTSQPRENFTRTLRHAFLREPQFAHFADDIIFAERGQGEEPDVAASRIFLVAKTTENKREEMSVLLDTLRRLSLTSRVRFLIFNPSFVYLDRYAAAVSSPLRHSLLAVLFLLGLSSLAVVEPLVSVWLGLTLLSVQFGVLGFMTLWGVELDCMSVLCLISALGHSADCSGPLLCGFAAGRGESRTRWVRVALERHGVPSLQTLICYGAALVPVGSIRSNLTRTLFRCLTLTAGCSALHTLAFLPTLLTFLPPSKSRGHGPGGEGQRQEVECVEMNDSTRVVDQITTV

Target ORF information:

RefSeq Version	XM_005449082.3
Organism	Oreochromis niloticus(Nile tilapia)
Definition	Oreochromis niloticus patched domain containing 1 (ptchd1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005449082.3

ORF Insert Sequence:

ATGCTCGGCA GTGCTCCGGG GCTTTCCGGT CGGTGGGAGA GGCTCGAGCC GCGGCCGCGC 
GCTAGGATGC TGCGCCAGGT GTTGCACGCG GGCCTGAGGA CCTCGTTCCA CGCGCTGGGT 
CGGTTCGTGG CGAGCCACCC GGTCTTCTTC GCCTCGGCAC CCGTGCTCCT CTCCATCTTG 
CTGGGTTCCA GCTTTAGCCG GTACCGCGTG GAAGAGGACG TGGAGAGCCT GCTCGCGCCC 
AAGCACAGCC TGGCCAAGAT AGAGGGCAAC CTCGTGGACA GCCTGTTCCC AGTGAACCGC 
TCCAAGCACG CGCTCTACTC TGACCTGCAG ACGCCGGGCC GCTATGGGCG CGTGATCATC 
ACCACCCGAA AGGGAAGCGT GCTGGATCCG GTTCATCTGG ACGCCGTACT AAAGCTCCAC 
AGACGGATCT ACCAGATGCA GGTAACCGTG CCAGCAACAG GCTTCAACAG TTTCAACTAC 
TCCTTCTCCA ACCTTTGCCT TCTTGATGAC AAGAATGTCT GCATCATCGA TGACATCATC 
CGCGCCATGG AGGAGATCCA GTCAGCTCGC AACTCCAATC GCTCCGTGCC CGTCCTGAGG 
TACCCCATCA CACAGCTGGC AGATGGGCGG CAAGCGTACA TTGGCCACCA GATGGGTGGT 
GTGCAGGGCT GGGGTCCTGG GGGGGCGATG CGAGTTCAGG GAATGGGAGT GAGGGGAGAA 
GGTGTCCGAT CTGCCCGGGC GCTGCAGCTC ACCTACTACC TCCAAGCCCC AGGCGGCCTG 
ATGGACCGGG TGGCCAGTCA GTGGGAAAAG GCCTTCTGTG CTGAGTTGCA GCACTTTACA 
GAGTTGCACC CAAAGTTGGG CCTCTACCCT TCTACTTCAT CTTCTCTGAG GACAGACTTC 
CAGTTCTCCT CAGTGCTGGC ACGTCGCCCC CTGTTGGCCA GCCTGGGAGT GTGTGGGGTG 
CTTGTCGTCC TTTGCTGCTC CATGAGGGAC TGTGTGAGGT CCAAACCCTG GCTGGGACTG 
CTAGCTCTGC TGTCCATCAC TCTATCAGGC CTTACTGCTG CTGGGATACT CAACCTGACT 
GGGGCCACCT ACAACTCCAC GTACTTGGGG ATCCCGTTCG TCATGCTTGG TCATGGGCTG 
TTCGGCTCCT TCGAGATGCT ATCATCGTGG AGACGAACCC GTGAGGACCA GCATGTGAAA 
GAGCGTGTTG CAAGTGTCTT CGAGGATGTT ATGTTGCGTT TCTCTGGCTC CACCATGATC 
CACCTGATGA CCTTGGGCCT GGCCGCCTCC CCGCTCACCA ACATGGAAGC CGTCCGGCTC 
TTCTGCCGCA CCGCTGCTCT GGCCGTTACC ATCAGCTATG TTTACATGTT GTCCTTCTAC 
AGTTCCTGTC TGGTGTTTAC AGGCTACCTG GAGACCGGGT ACAGACATGG CTGCTTCTGC 
CGGCGAGTCC CCAAACCGGA CCGTTTGGAC TCTAAACCGG CTTGGTACAG GTGTTTAATG 
TACACTCGTT ACCAGGAGGA GACCCAAGTG ACGAATCCTC CACACGGGAT TGGTCACACT 
CACACACCAA ACCCTGACCT TACTCACACG CACACGCACC CTCACCCGCA TACAATGAAC 
AACCCAGCTG TTCGTGGACA TGCTCATGTT GCTAACTCCA CTCACCCACA CCCACACCCT 
CACCCACACC CTCACCCACA TTCCACAGCA AGCATGGACC CTCATCCTCA GGACTCACAC 
CTTTTGTTGG GCTGTGTGCG GCGTTGCTAT GGAGACTGGA TCACTAACAC TTATGTTAAA 
CCCTTTGTGG TTCTGCTGTA TCTGGTTTAC ATCTCATTTG GATTGATGGG ATTCCTACAG 
GTGACCCAGG GTTCTGACCC CAGCGCACTG GTTGCAATGG ACACCGCAAC AGTATTGTAC 
ACCCGAGCCC AGCAGCGTTA CTTCAGCTCA TACTCTCCTG TGATTGGATT CTACATTTAT 
GAAAGTGCCC CTTACTGGAA TGCCTCAGTG CAGCGGGATC TGCTGGAATA TGCCAAAGGC 
TTCCAGAGAA TAAGCTGGCT GGAAGCTTAC CTGAACTACC TGTCGGATCG CAACCAGTCC 
ACCAGCCAGC CGCGGGAAAA TTTCACCCGC ACGCTACGCC ACGCCTTTCT CCGTGAGCCG 
CAGTTTGCTC ACTTTGCGGA TGACATTATA TTTGCAGAGC GCGGCCAGGG CGAAGAGCCT 
GATGTGGCAG CTTCTCGCAT ATTCTTGGTT GCCAAAACAA CGGAGAACAA GCGCGAGGAG 
ATGTCAGTGC TGCTGGACAC GCTGCGACGC CTGTCACTGA CCTCACGAGT CCGCTTCTTA 
ATCTTTAACC CTTCTTTCGT CTACCTGGAC CGCTATGCTG CGGCGGTCAG CTCCCCACTT 
AGACACTCAC TGCTGGCGGT GCTCTTCCTG TTGGGTCTCT CCTCTCTGGC TGTTGTGGAG 
CCCCTGGTGT CAGTGTGGCT GGGCCTCACC CTGCTCTCTG TCCAGTTTGG CGTCCTGGGC 
TTCATGACCT TATGGGGTGT AGAGCTGGAT TGCATGTCTG TGCTATGTTT GATCTCAGCT 
TTGGGGCACT CAGCAGACTG CAGCGGCCCT CTCCTCTGTG GCTTTGCTGC AGGTCGGGGT 
GAAAGCAGGA CTCGCTGGGT GAGAGTGGCA CTGGAGAGAC ACGGGGTTCC CTCTCTGCAG 
ACTCTCATCT GTTACGGCGC TGCCCTGGTA CCCGTTGGCT CCATACGCTC CAACCTCACA 
CGCACACTAT TTCGCTGCCT CACCCTAACA GCTGGTTGCT CGGCCCTGCA CACGCTGGCA 
TTCCTGCCCA CACTCCTCAC CTTCCTGCCC CCCTCCAAGA GCCGGGGCCA TGGACCTGGA 
GGGGAAGGAC AGAGACAGGA GGTGGAGTGT GTTGAAATGA ATGACAGCAC CAGAGTTGTC 
GACCAGATCA CCACCGTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ptchd1 ( XM_025910525.1 ) cDNA ORF clone, Oreochromis niloticus(Nile tilapia) -> XP_025766310.1 Oreochromis niloticus patched domain containing 1 (ptchd1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OOr71679
Clone ID Related Accession (Same CDS sequence)	XM_005449082.3 , XM_025910525.1 , XM_005449082.2
Accession Version	XM_025910525.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2961bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-07-25
Organism	Oreochromis niloticus(Nile tilapia)
Product	patched domain-containing protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_031974.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Oreochromis niloticus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCGGCA GTGCTCCGGG GCTTTCCGGT CGGTGGGAGA GGCTCGAGCC GCGGCCGCGC 
GCTAGGATGC TGCGCCAGGT GTTGCACGCG GGCCTGAGGA CCTCGTTCCA CGCGCTGGGT 
CGGTTCGTGG CGAGCCACCC GGTCTTCTTC GCCTCGGCAC CCGTGCTCCT CTCCATCTTG 
CTGGGTTCCA GCTTTAGCCG GTACCGCGTG GAAGAGGACG TGGAGAGCCT GCTCGCGCCC 
AAGCACAGCC TGGCCAAGAT AGAGGGCAAC CTCGTGGACA GCCTGTTCCC AGTGAACCGC 
TCCAAGCACG CGCTCTACTC TGACCTGCAG ACGCCGGGCC GCTATGGGCG CGTGATCATC 
ACCACCCGAA AGGGAAGCGT GCTGGATCCG GTTCATCTGG ACGCCGTACT AAAGCTCCAC 
AGACGGATCT ACCAGATGCA GGTAACCGTG CCAGCAACAG GCTTCAACAG TTTCAACTAC 
TCCTTCTCCA ACCTTTGCCT TCTTGATGAC AAGAATGTCT GCATCATCGA TGACATCATC 
CGCGCCATGG AGGAGATCCA GTCAGCTCGC AACTCCAATC GCTCCGTGCC CGTCCTGAGG 
TACCCCATCA CACAGCTGGC AGATGGGCGG CAAGCGTACA TTGGCCACCA GATGGGTGGT 
GTGCAGGGCT GGGGTCCTGG GGGGGCGATG CGAGTTCAGG GAATGGGAGT GAGGGGAGAA 
GGTGTCCGAT CTGCCCGGGC GCTGCAGCTC ACCTACTACC TCCAAGCCCC AGGCGGCCTG 
ATGGACCGGG TGGCCAGTCA GTGGGAAAAG GCCTTCTGTG CTGAGTTGCA GCACTTTACA 
GAGTTGCACC CAAAGTTGGG CCTCTACCCT TCTACTTCAT CTTCTCTGAG GACAGACTTC 
CAGTTCTCCT CAGTGCTGGC ACGTCGCCCC CTGTTGGCCA GCCTGGGAGT GTGTGGGGTG 
CTTGTCGTCC TTTGCTGCTC CATGAGGGAC TGTGTGAGGT CCAAACCCTG GCTGGGACTG 
CTAGCTCTGC TGTCCATCAC TCTATCAGGC CTTACTGCTG CTGGGATACT CAACCTGACT 
GGGGCCACCT ACAACTCCAC GTACTTGGGG ATCCCGTTCG TCATGCTTGG TCATGGGCTG 
TTCGGCTCCT TCGAGATGCT ATCATCGTGG AGACGAACCC GTGAGGACCA GCATGTGAAA 
GAGCGTGTTG CAAGTGTCTT CGAGGATGTT ATGTTGCGTT TCTCTGGCTC CACCATGATC 
CACCTGATGA CCTTGGGCCT GGCCGCCTCC CCGCTCACCA ACATGGAAGC CGTCCGGCTC 
TTCTGCCGCA CCGCTGCTCT GGCCGTTACC ATCAGCTATG TTTACATGTT GTCCTTCTAC 
AGTTCCTGTC TGGTGTTTAC AGGCTACCTG GAGACCGGGT ACAGACATGG CTGCTTCTGC 
CGGCGAGTCC CCAAACCGGA CCGTTTGGAC TCTAAACCGG CTTGGTACAG GTGTTTAATG 
TACACTCGTT ACCAGGAGGA GACCCAAGTG ACGAATCCTC CACACGGGAT TGGTCACACT 
CACACACCAA ACCCTGACCT TACTCACACG CACACGCACC CTCACCCGCA TACAATGAAC 
AACCCAGCTG TTCGTGGACA TGCTCATGTT GCTAACTCCA CTCACCCACA CCCACACCCT 
CACCCACACC CTCACCCACA TTCCACAGCA AGCATGGACC CTCATCCTCA GGACTCACAC 
CTTTTGTTGG GCTGTGTGCG GCGTTGCTAT GGAGACTGGA TCACTAACAC TTATGTTAAA 
CCCTTTGTGG TTCTGCTGTA TCTGGTTTAC ATCTCATTTG GATTGATGGG ATTCCTACAG 
GTGACCCAGG GTTCTGACCC CAGCGCACTG GTTGCAATGG ACACCGCAAC AGTATTGTAC 
ACCCGAGCCC AGCAGCGTTA CTTCAGCTCA TACTCTCCTG TGATTGGATT CTACATTTAT 
GAAAGTGCCC CTTACTGGAA TGCCTCAGTG CAGCGGGATC TGCTGGAATA TGCCAAAGGC 
TTCCAGAGAA TAAGCTGGCT GGAAGCTTAC CTGAACTACC TGTCGGATCG CAACCAGTCC 
ACCAGCCAGC CGCGGGAAAA TTTCACCCGC ACGCTACGCC ACGCCTTTCT CCGTGAGCCG 
CAGTTTGCTC ACTTTGCGGA TGACATTATA TTTGCAGAGC GCGGCCAGGG CGAAGAGCCT 
GATGTGGCAG CTTCTCGCAT ATTCTTGGTT GCCAAAACAA CGGAGAACAA GCGCGAGGAG 
ATGTCAGTGC TGCTGGACAC GCTGCGACGC CTGTCACTGA CCTCACGAGT CCGCTTCTTA 
ATCTTTAACC CTTCTTTCGT CTACCTGGAC CGCTATGCTG CGGCGGTCAG CTCCCCACTT 
AGACACTCAC TGCTGGCGGT GCTCTTCCTG TTGGGTCTCT CCTCTCTGGC TGTTGTGGAG 
CCCCTGGTGT CAGTGTGGCT GGGCCTCACC CTGCTCTCTG TCCAGTTTGG CGTCCTGGGC 
TTCATGACCT TATGGGGTGT AGAGCTGGAT TGCATGTCTG TGCTATGTTT GATCTCAGCT 
TTGGGGCACT CAGCAGACTG CAGCGGCCCT CTCCTCTGTG GCTTTGCTGC AGGTCGGGGT 
GAAAGCAGGA CTCGCTGGGT GAGAGTGGCA CTGGAGAGAC ACGGGGTTCC CTCTCTGCAG 
ACTCTCATCT GTTACGGCGC TGCCCTGGTA CCCGTTGGCT CCATACGCTC CAACCTCACA 
CGCACACTAT TTCGCTGCCT CACCCTAACA GCTGGTTGCT CGGCCCTGCA CACGCTGGCA 
TTCCTGCCCA CACTCCTCAC CTTCCTGCCC CCCTCCAAGA GCCGGGGCCA TGGACCTGGA 
GGGGAAGGAC AGAGACAGGA GGTGGAGTGT GTTGAAATGA ATGACAGCAC CAGAGTTGTC 
GACCAGATCA CCACCGTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025766310.1
CDS	1379..4339
Translation	MLGSAPGLSGRWERLEPRPRARMLRQVLHAGLRTSFHALGRFVASHPVFFASAPVLLSILLGSSFSRYRVEEDVESLLAPKHSLAKIEGNLVDSLFPVNRSKHALYSDLQTPGRYGRVIITTRKGSVLDPVHLDAVLKLHRRIYQMQVTVPATGFNSFNYSFSNLCLLDDKNVCIIDDIIRAMEEIQSARNSNRSVPVLRYPITQLADGRQAYIGHQMGGVQGWGPGGAMRVQGMGVRGEGVRSARALQLTYYLQAPGGLMDRVASQWEKAFCAELQHFTELHPKLGLYPSTSSSLRTDFQFSSVLARRPLLASLGVCGVLVVLCCSMRDCVRSKPWLGLLALLSITLSGLTAAGILNLTGATYNSTYLGIPFVMLGHGLFGSFEMLSSWRRTREDQHVKERVASVFEDVMLRFSGSTMIHLMTLGLAASPLTNMEAVRLFCRTAALAVTISYVYMLSFYSSCLVFTGYLETGYRHGCFCRRVPKPDRLDSKPAWYRCLMYTRYQEETQVTNPPHGIGHTHTPNPDLTHTHTHPHPHTMNNPAVRGHAHVANSTHPHPHPHPHPHPHSTASMDPHPQDSHLLLGCVRRCYGDWITNTYVKPFVVLLYLVYISFGLMGFLQVTQGSDPSALVAMDTATVLYTRAQQRYFSSYSPVIGFYIYESAPYWNASVQRDLLEYAKGFQRISWLEAYLNYLSDRNQSTSQPRENFTRTLRHAFLREPQFAHFADDIIFAERGQGEEPDVAASRIFLVAKTTENKREEMSVLLDTLRRLSLTSRVRFLIFNPSFVYLDRYAAAVSSPLRHSLLAVLFLLGLSSLAVVEPLVSVWLGLTLLSVQFGVLGFMTLWGVELDCMSVLCLISALGHSADCSGPLLCGFAAGRGESRTRWVRVALERHGVPSLQTLICYGAALVPVGSIRSNLTRTLFRCLTLTAGCSALHTLAFLPTLLTFLPPSKSRGHGPGGEGQRQEVECVEMNDSTRVVDQITTV

Target ORF information:

RefSeq Version	XM_025910525.1
Organism	Oreochromis niloticus(Nile tilapia)
Definition	Oreochromis niloticus patched domain containing 1 (ptchd1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025910525.1

ORF Insert Sequence:

ATGCTCGGCA GTGCTCCGGG GCTTTCCGGT CGGTGGGAGA GGCTCGAGCC GCGGCCGCGC 
GCTAGGATGC TGCGCCAGGT GTTGCACGCG GGCCTGAGGA CCTCGTTCCA CGCGCTGGGT 
CGGTTCGTGG CGAGCCACCC GGTCTTCTTC GCCTCGGCAC CCGTGCTCCT CTCCATCTTG 
CTGGGTTCCA GCTTTAGCCG GTACCGCGTG GAAGAGGACG TGGAGAGCCT GCTCGCGCCC 
AAGCACAGCC TGGCCAAGAT AGAGGGCAAC CTCGTGGACA GCCTGTTCCC AGTGAACCGC 
TCCAAGCACG CGCTCTACTC TGACCTGCAG ACGCCGGGCC GCTATGGGCG CGTGATCATC 
ACCACCCGAA AGGGAAGCGT GCTGGATCCG GTTCATCTGG ACGCCGTACT AAAGCTCCAC 
AGACGGATCT ACCAGATGCA GGTAACCGTG CCAGCAACAG GCTTCAACAG TTTCAACTAC 
TCCTTCTCCA ACCTTTGCCT TCTTGATGAC AAGAATGTCT GCATCATCGA TGACATCATC 
CGCGCCATGG AGGAGATCCA GTCAGCTCGC AACTCCAATC GCTCCGTGCC CGTCCTGAGG 
TACCCCATCA CACAGCTGGC AGATGGGCGG CAAGCGTACA TTGGCCACCA GATGGGTGGT 
GTGCAGGGCT GGGGTCCTGG GGGGGCGATG CGAGTTCAGG GAATGGGAGT GAGGGGAGAA 
GGTGTCCGAT CTGCCCGGGC GCTGCAGCTC ACCTACTACC TCCAAGCCCC AGGCGGCCTG 
ATGGACCGGG TGGCCAGTCA GTGGGAAAAG GCCTTCTGTG CTGAGTTGCA GCACTTTACA 
GAGTTGCACC CAAAGTTGGG CCTCTACCCT TCTACTTCAT CTTCTCTGAG GACAGACTTC 
CAGTTCTCCT CAGTGCTGGC ACGTCGCCCC CTGTTGGCCA GCCTGGGAGT GTGTGGGGTG 
CTTGTCGTCC TTTGCTGCTC CATGAGGGAC TGTGTGAGGT CCAAACCCTG GCTGGGACTG 
CTAGCTCTGC TGTCCATCAC TCTATCAGGC CTTACTGCTG CTGGGATACT CAACCTGACT 
GGGGCCACCT ACAACTCCAC GTACTTGGGG ATCCCGTTCG TCATGCTTGG TCATGGGCTG 
TTCGGCTCCT TCGAGATGCT ATCATCGTGG AGACGAACCC GTGAGGACCA GCATGTGAAA 
GAGCGTGTTG CAAGTGTCTT CGAGGATGTT ATGTTGCGTT TCTCTGGCTC CACCATGATC 
CACCTGATGA CCTTGGGCCT GGCCGCCTCC CCGCTCACCA ACATGGAAGC CGTCCGGCTC 
TTCTGCCGCA CCGCTGCTCT GGCCGTTACC ATCAGCTATG TTTACATGTT GTCCTTCTAC 
AGTTCCTGTC TGGTGTTTAC AGGCTACCTG GAGACCGGGT ACAGACATGG CTGCTTCTGC 
CGGCGAGTCC CCAAACCGGA CCGTTTGGAC TCTAAACCGG CTTGGTACAG GTGTTTAATG 
TACACTCGTT ACCAGGAGGA GACCCAAGTG ACGAATCCTC CACACGGGAT TGGTCACACT 
CACACACCAA ACCCTGACCT TACTCACACG CACACGCACC CTCACCCGCA TACAATGAAC 
AACCCAGCTG TTCGTGGACA TGCTCATGTT GCTAACTCCA CTCACCCACA CCCACACCCT 
CACCCACACC CTCACCCACA TTCCACAGCA AGCATGGACC CTCATCCTCA GGACTCACAC 
CTTTTGTTGG GCTGTGTGCG GCGTTGCTAT GGAGACTGGA TCACTAACAC TTATGTTAAA 
CCCTTTGTGG TTCTGCTGTA TCTGGTTTAC ATCTCATTTG GATTGATGGG ATTCCTACAG 
GTGACCCAGG GTTCTGACCC CAGCGCACTG GTTGCAATGG ACACCGCAAC AGTATTGTAC 
ACCCGAGCCC AGCAGCGTTA CTTCAGCTCA TACTCTCCTG TGATTGGATT CTACATTTAT 
GAAAGTGCCC CTTACTGGAA TGCCTCAGTG CAGCGGGATC TGCTGGAATA TGCCAAAGGC 
TTCCAGAGAA TAAGCTGGCT GGAAGCTTAC CTGAACTACC TGTCGGATCG CAACCAGTCC 
ACCAGCCAGC CGCGGGAAAA TTTCACCCGC ACGCTACGCC ACGCCTTTCT CCGTGAGCCG 
CAGTTTGCTC ACTTTGCGGA TGACATTATA TTTGCAGAGC GCGGCCAGGG CGAAGAGCCT 
GATGTGGCAG CTTCTCGCAT ATTCTTGGTT GCCAAAACAA CGGAGAACAA GCGCGAGGAG 
ATGTCAGTGC TGCTGGACAC GCTGCGACGC CTGTCACTGA CCTCACGAGT CCGCTTCTTA 
ATCTTTAACC CTTCTTTCGT CTACCTGGAC CGCTATGCTG CGGCGGTCAG CTCCCCACTT 
AGACACTCAC TGCTGGCGGT GCTCTTCCTG TTGGGTCTCT CCTCTCTGGC TGTTGTGGAG 
CCCCTGGTGT CAGTGTGGCT GGGCCTCACC CTGCTCTCTG TCCAGTTTGG CGTCCTGGGC 
TTCATGACCT TATGGGGTGT AGAGCTGGAT TGCATGTCTG TGCTATGTTT GATCTCAGCT 
TTGGGGCACT CAGCAGACTG CAGCGGCCCT CTCCTCTGTG GCTTTGCTGC AGGTCGGGGT 
GAAAGCAGGA CTCGCTGGGT GAGAGTGGCA CTGGAGAGAC ACGGGGTTCC CTCTCTGCAG 
ACTCTCATCT GTTACGGCGC TGCCCTGGTA CCCGTTGGCT CCATACGCTC CAACCTCACA 
CGCACACTAT TTCGCTGCCT CACCCTAACA GCTGGTTGCT CGGCCCTGCA CACGCTGGCA 
TTCCTGCCCA CACTCCTCAC CTTCCTGCCC CCCTCCAAGA GCCGGGGCCA TGGACCTGGA 
GGGGAAGGAC AGAGACAGGA GGTGGAGTGT GTTGAAATGA ATGACAGCAC CAGAGTTGTC 
GACCAGATCA CCACCGTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ptchd1 ( XM_005449082.2 ) cDNA ORF clone, Oreochromis niloticus(Nile tilapia) -> XP_005449139.1 Oreochromis niloticus patched domain containing 1 (ptchd1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OOr71679
Clone ID Related Accession (Same CDS sequence)	XM_005449082.3 , XM_025910525.1 , XM_005449082.2
Accession Version	XM_005449082.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2961bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-12-04
Organism	Oreochromis niloticus(Nile tilapia)
Product	patched domain-containing protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_031974.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 5, 2016 this sequence version replaced XM_005449082.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oreochromis niloticus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCGGCA GTGCTCCGGG GCTTTCCGGT CGGTGGGAGA GGCTCGAGCC GCGGCCGCGC 
GCTAGGATGC TGCGCCAGGT GTTGCACGCG GGCCTGAGGA CCTCGTTCCA CGCGCTGGGT 
CGGTTCGTGG CGAGCCACCC GGTCTTCTTC GCCTCGGCAC CCGTGCTCCT CTCCATCTTG 
CTGGGTTCCA GCTTTAGCCG GTACCGCGTG GAAGAGGACG TGGAGAGCCT GCTCGCGCCC 
AAGCACAGCC TGGCCAAGAT AGAGGGCAAC CTCGTGGACA GCCTGTTCCC AGTGAACCGC 
TCCAAGCACG CGCTCTACTC TGACCTGCAG ACGCCGGGCC GCTATGGGCG CGTGATCATC 
ACCACCCGAA AGGGAAGCGT GCTGGATCCG GTTCATCTGG ACGCCGTACT AAAGCTCCAC 
AGACGGATCT ACCAGATGCA GGTAACCGTG CCAGCAACAG GCTTCAACAG TTTCAACTAC 
TCCTTCTCCA ACCTTTGCCT TCTTGATGAC AAGAATGTCT GCATCATCGA TGACATCATC 
CGCGCCATGG AGGAGATCCA GTCAGCTCGC AACTCCAATC GCTCCGTGCC CGTCCTGAGG 
TACCCCATCA CACAGCTGGC AGATGGGCGG CAAGCGTACA TTGGCCACCA GATGGGTGGT 
GTGCAGGGCT GGGGTCCTGG GGGGGCGATG CGAGTTCAGG GAATGGGAGT GAGGGGAGAA 
GGTGTCCGAT CTGCCCGGGC GCTGCAGCTC ACCTACTACC TCCAAGCCCC AGGCGGCCTG 
ATGGACCGGG TGGCCAGTCA GTGGGAAAAG GCCTTCTGTG CTGAGTTGCA GCACTTTACA 
GAGTTGCACC CAAAGTTGGG CCTCTACCCT TCTACTTCAT CTTCTCTGAG GACAGACTTC 
CAGTTCTCCT CAGTGCTGGC ACGTCGCCCC CTGTTGGCCA GCCTGGGAGT GTGTGGGGTG 
CTTGTCGTCC TTTGCTGCTC CATGAGGGAC TGTGTGAGGT CCAAACCCTG GCTGGGACTG 
CTAGCTCTGC TGTCCATCAC TCTATCAGGC CTTACTGCTG CTGGGATACT CAACCTGACT 
GGGGCCACCT ACAACTCCAC GTACTTGGGG ATCCCGTTCG TCATGCTTGG TCATGGGCTG 
TTCGGCTCCT TCGAGATGCT ATCATCGTGG AGACGAACCC GTGAGGACCA GCATGTGAAA 
GAGCGTGTTG CAAGTGTCTT CGAGGATGTT ATGTTGCGTT TCTCTGGCTC CACCATGATC 
CACCTGATGA CCTTGGGCCT GGCCGCCTCC CCGCTCACCA ACATGGAAGC CGTCCGGCTC 
TTCTGCCGCA CCGCTGCTCT GGCCGTTACC ATCAGCTATG TTTACATGTT GTCCTTCTAC 
AGTTCCTGTC TGGTGTTTAC AGGCTACCTG GAGACCGGGT ACAGACATGG CTGCTTCTGC 
CGGCGAGTCC CCAAACCGGA CCGTTTGGAC TCTAAACCGG CTTGGTACAG GTGTTTAATG 
TACACTCGTT ACCAGGAGGA GACCCAAGTG ACGAATCCTC CACACGGGAT TGGTCACACT 
CACACACCAA ACCCTGACCT TACTCACACG CACACGCACC CTCACCCGCA TACAATGAAC 
AACCCAGCTG TTCGTGGACA TGCTCATGTT GCTAACTCCA CTCACCCACA CCCACACCCT 
CACCCACACC CTCACCCACA TTCCACAGCA AGCATGGACC CTCATCCTCA GGACTCACAC 
CTTTTGTTGG GCTGTGTGCG GCGTTGCTAT GGAGACTGGA TCACTAACAC TTATGTTAAA 
CCCTTTGTGG TTCTGCTGTA TCTGGTTTAC ATCTCATTTG GATTGATGGG ATTCCTACAG 
GTGACCCAGG GTTCTGACCC CAGCGCACTG GTTGCAATGG ACACCGCAAC AGTATTGTAC 
ACCCGAGCCC AGCAGCGTTA CTTCAGCTCA TACTCTCCTG TGATTGGATT CTACATTTAT 
GAAAGTGCCC CTTACTGGAA TGCCTCAGTG CAGCGGGATC TGCTGGAATA TGCCAAAGGC 
TTCCAGAGAA TAAGCTGGCT GGAAGCTTAC CTGAACTACC TGTCGGATCG CAACCAGTCC 
ACCAGCCAGC CGCGGGAAAA TTTCACCCGC ACGCTACGCC ACGCCTTTCT CCGTGAGCCG 
CAGTTTGCTC ACTTTGCGGA TGACATTATA TTTGCAGAGC GCGGCCAGGG CGAAGAGCCT 
GATGTGGCAG CTTCTCGCAT ATTCTTGGTT GCCAAAACAA CGGAGAACAA GCGCGAGGAG 
ATGTCAGTGC TGCTGGACAC GCTGCGACGC CTGTCACTGA CCTCACGAGT CCGCTTCTTA 
ATCTTTAACC CTTCTTTCGT CTACCTGGAC CGCTATGCTG CGGCGGTCAG CTCCCCACTT 
AGACACTCAC TGCTGGCGGT GCTCTTCCTG TTGGGTCTCT CCTCTCTGGC TGTTGTGGAG 
CCCCTGGTGT CAGTGTGGCT GGGCCTCACC CTGCTCTCTG TCCAGTTTGG CGTCCTGGGC 
TTCATGACCT TATGGGGTGT AGAGCTGGAT TGCATGTCTG TGCTATGTTT GATCTCAGCT 
TTGGGGCACT CAGCAGACTG CAGCGGCCCT CTCCTCTGTG GCTTTGCTGC AGGTCGGGGT 
GAAAGCAGGA CTCGCTGGGT GAGAGTGGCA CTGGAGAGAC ACGGGGTTCC CTCTCTGCAG 
ACTCTCATCT GTTACGGCGC TGCCCTGGTA CCCGTTGGCT CCATACGCTC CAACCTCACA 
CGCACACTAT TTCGCTGCCT CACCCTAACA GCTGGTTGCT CGGCCCTGCA CACGCTGGCA 
TTCCTGCCCA CACTCCTCAC CTTCCTGCCC CCCTCCAAGA GCCGGGGCCA TGGACCTGGA 
GGGGAAGGAC AGAGACAGGA GGTGGAGTGT GTTGAAATGA ATGACAGCAC CAGAGTTGTC 
GACCAGATCA CCACCGTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005449139.1
CDS	1248..4208
Translation	MLGSAPGLSGRWERLEPRPRARMLRQVLHAGLRTSFHALGRFVASHPVFFASAPVLLSILLGSSFSRYRVEEDVESLLAPKHSLAKIEGNLVDSLFPVNRSKHALYSDLQTPGRYGRVIITTRKGSVLDPVHLDAVLKLHRRIYQMQVTVPATGFNSFNYSFSNLCLLDDKNVCIIDDIIRAMEEIQSARNSNRSVPVLRYPITQLADGRQAYIGHQMGGVQGWGPGGAMRVQGMGVRGEGVRSARALQLTYYLQAPGGLMDRVASQWEKAFCAELQHFTELHPKLGLYPSTSSSLRTDFQFSSVLARRPLLASLGVCGVLVVLCCSMRDCVRSKPWLGLLALLSITLSGLTAAGILNLTGATYNSTYLGIPFVMLGHGLFGSFEMLSSWRRTREDQHVKERVASVFEDVMLRFSGSTMIHLMTLGLAASPLTNMEAVRLFCRTAALAVTISYVYMLSFYSSCLVFTGYLETGYRHGCFCRRVPKPDRLDSKPAWYRCLMYTRYQEETQVTNPPHGIGHTHTPNPDLTHTHTHPHPHTMNNPAVRGHAHVANSTHPHPHPHPHPHPHSTASMDPHPQDSHLLLGCVRRCYGDWITNTYVKPFVVLLYLVYISFGLMGFLQVTQGSDPSALVAMDTATVLYTRAQQRYFSSYSPVIGFYIYESAPYWNASVQRDLLEYAKGFQRISWLEAYLNYLSDRNQSTSQPRENFTRTLRHAFLREPQFAHFADDIIFAERGQGEEPDVAASRIFLVAKTTENKREEMSVLLDTLRRLSLTSRVRFLIFNPSFVYLDRYAAAVSSPLRHSLLAVLFLLGLSSLAVVEPLVSVWLGLTLLSVQFGVLGFMTLWGVELDCMSVLCLISALGHSADCSGPLLCGFAAGRGESRTRWVRVALERHGVPSLQTLICYGAALVPVGSIRSNLTRTLFRCLTLTAGCSALHTLAFLPTLLTFLPPSKSRGHGPGGEGQRQEVECVEMNDSTRVVDQITTV

Target ORF information:

RefSeq Version	XM_005449082.2
Organism	Oreochromis niloticus(Nile tilapia)
Definition	Oreochromis niloticus patched domain containing 1 (ptchd1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005449082.2

ORF Insert Sequence:

ATGCTCGGCA GTGCTCCGGG GCTTTCCGGT CGGTGGGAGA GGCTCGAGCC GCGGCCGCGC 
GCTAGGATGC TGCGCCAGGT GTTGCACGCG GGCCTGAGGA CCTCGTTCCA CGCGCTGGGT 
CGGTTCGTGG CGAGCCACCC GGTCTTCTTC GCCTCGGCAC CCGTGCTCCT CTCCATCTTG 
CTGGGTTCCA GCTTTAGCCG GTACCGCGTG GAAGAGGACG TGGAGAGCCT GCTCGCGCCC 
AAGCACAGCC TGGCCAAGAT AGAGGGCAAC CTCGTGGACA GCCTGTTCCC AGTGAACCGC 
TCCAAGCACG CGCTCTACTC TGACCTGCAG ACGCCGGGCC GCTATGGGCG CGTGATCATC 
ACCACCCGAA AGGGAAGCGT GCTGGATCCG GTTCATCTGG ACGCCGTACT AAAGCTCCAC 
AGACGGATCT ACCAGATGCA GGTAACCGTG CCAGCAACAG GCTTCAACAG TTTCAACTAC 
TCCTTCTCCA ACCTTTGCCT TCTTGATGAC AAGAATGTCT GCATCATCGA TGACATCATC 
CGCGCCATGG AGGAGATCCA GTCAGCTCGC AACTCCAATC GCTCCGTGCC CGTCCTGAGG 
TACCCCATCA CACAGCTGGC AGATGGGCGG CAAGCGTACA TTGGCCACCA GATGGGTGGT 
GTGCAGGGCT GGGGTCCTGG GGGGGCGATG CGAGTTCAGG GAATGGGAGT GAGGGGAGAA 
GGTGTCCGAT CTGCCCGGGC GCTGCAGCTC ACCTACTACC TCCAAGCCCC AGGCGGCCTG 
ATGGACCGGG TGGCCAGTCA GTGGGAAAAG GCCTTCTGTG CTGAGTTGCA GCACTTTACA 
GAGTTGCACC CAAAGTTGGG CCTCTACCCT TCTACTTCAT CTTCTCTGAG GACAGACTTC 
CAGTTCTCCT CAGTGCTGGC ACGTCGCCCC CTGTTGGCCA GCCTGGGAGT GTGTGGGGTG 
CTTGTCGTCC TTTGCTGCTC CATGAGGGAC TGTGTGAGGT CCAAACCCTG GCTGGGACTG 
CTAGCTCTGC TGTCCATCAC TCTATCAGGC CTTACTGCTG CTGGGATACT CAACCTGACT 
GGGGCCACCT ACAACTCCAC GTACTTGGGG ATCCCGTTCG TCATGCTTGG TCATGGGCTG 
TTCGGCTCCT TCGAGATGCT ATCATCGTGG AGACGAACCC GTGAGGACCA GCATGTGAAA 
GAGCGTGTTG CAAGTGTCTT CGAGGATGTT ATGTTGCGTT TCTCTGGCTC CACCATGATC 
CACCTGATGA CCTTGGGCCT GGCCGCCTCC CCGCTCACCA ACATGGAAGC CGTCCGGCTC 
TTCTGCCGCA CCGCTGCTCT GGCCGTTACC ATCAGCTATG TTTACATGTT GTCCTTCTAC 
AGTTCCTGTC TGGTGTTTAC AGGCTACCTG GAGACCGGGT ACAGACATGG CTGCTTCTGC 
CGGCGAGTCC CCAAACCGGA CCGTTTGGAC TCTAAACCGG CTTGGTACAG GTGTTTAATG 
TACACTCGTT ACCAGGAGGA GACCCAAGTG ACGAATCCTC CACACGGGAT TGGTCACACT 
CACACACCAA ACCCTGACCT TACTCACACG CACACGCACC CTCACCCGCA TACAATGAAC 
AACCCAGCTG TTCGTGGACA TGCTCATGTT GCTAACTCCA CTCACCCACA CCCACACCCT 
CACCCACACC CTCACCCACA TTCCACAGCA AGCATGGACC CTCATCCTCA GGACTCACAC 
CTTTTGTTGG GCTGTGTGCG GCGTTGCTAT GGAGACTGGA TCACTAACAC TTATGTTAAA 
CCCTTTGTGG TTCTGCTGTA TCTGGTTTAC ATCTCATTTG GATTGATGGG ATTCCTACAG 
GTGACCCAGG GTTCTGACCC CAGCGCACTG GTTGCAATGG ACACCGCAAC AGTATTGTAC 
ACCCGAGCCC AGCAGCGTTA CTTCAGCTCA TACTCTCCTG TGATTGGATT CTACATTTAT 
GAAAGTGCCC CTTACTGGAA TGCCTCAGTG CAGCGGGATC TGCTGGAATA TGCCAAAGGC 
TTCCAGAGAA TAAGCTGGCT GGAAGCTTAC CTGAACTACC TGTCGGATCG CAACCAGTCC 
ACCAGCCAGC CGCGGGAAAA TTTCACCCGC ACGCTACGCC ACGCCTTTCT CCGTGAGCCG 
CAGTTTGCTC ACTTTGCGGA TGACATTATA TTTGCAGAGC GCGGCCAGGG CGAAGAGCCT 
GATGTGGCAG CTTCTCGCAT ATTCTTGGTT GCCAAAACAA CGGAGAACAA GCGCGAGGAG 
ATGTCAGTGC TGCTGGACAC GCTGCGACGC CTGTCACTGA CCTCACGAGT CCGCTTCTTA 
ATCTTTAACC CTTCTTTCGT CTACCTGGAC CGCTATGCTG CGGCGGTCAG CTCCCCACTT 
AGACACTCAC TGCTGGCGGT GCTCTTCCTG TTGGGTCTCT CCTCTCTGGC TGTTGTGGAG 
CCCCTGGTGT CAGTGTGGCT GGGCCTCACC CTGCTCTCTG TCCAGTTTGG CGTCCTGGGC 
TTCATGACCT TATGGGGTGT AGAGCTGGAT TGCATGTCTG TGCTATGTTT GATCTCAGCT 
TTGGGGCACT CAGCAGACTG CAGCGGCCCT CTCCTCTGTG GCTTTGCTGC AGGTCGGGGT 
GAAAGCAGGA CTCGCTGGGT GAGAGTGGCA CTGGAGAGAC ACGGGGTTCC CTCTCTGCAG 
ACTCTCATCT GTTACGGCGC TGCCCTGGTA CCCGTTGGCT CCATACGCTC CAACCTCACA 
CGCACACTAT TTCGCTGCCT CACCCTAACA GCTGGTTGCT CGGCCCTGCA CACGCTGGCA 
TTCCTGCCCA CACTCCTCAC CTTCCTGCCC CCCTCCAAGA GCCGGGGCCA TGGACCTGGA 
GGGGAAGGAC AGAGACAGGA GGTGGAGTGT GTTGAAATGA ATGACAGCAC CAGAGTTGTC 
GACCAGATCA CCACCGTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ptchd1 ( XM_005449084.3 ) cDNA ORF clone, Oreochromis niloticus(Nile tilapia) -> XP_005449141.1 Oreochromis niloticus patched domain containing 1 (ptchd1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OOr71680
Clone ID Related Accession (Same CDS sequence)	XM_005449084.3 , XM_005449084.4
Accession Version	XM_005449084.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2526bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-12-04
Organism	Oreochromis niloticus(Nile tilapia)
Product	patched domain-containing protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_031974.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 5, 2016 this sequence version replaced XM_005449084.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oreochromis niloticus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGGTAA CCGTGCCAGC AACAGGCTTC AACAGTTTCA ACTACTCCTT CTCCAACCTT 
TGCCTTCTTG ATGACAAGAA TGTCTGCATC ATCGATGACA TCATCCGCGC CATGGAGGAG 
ATCCAGTCAG CTCGCAACTC CAATCGCTCC GTGCCCGTCC TGAGGTACCC CATCACACAG 
CTGGCAGATG GGCGGCAAGC GTACATTGGC CACCAGATGG GTGGTGTGCA GGGCTGGGGT 
CCTGGGGGGG CGATGCGAGT TCAGGGAATG GGAGTGAGGG GAGAAGGTGT CCGATCTGCC 
CGGGCGCTGC AGCTCACCTA CTACCTCCAA GCCCCAGGCG GCCTGATGGA CCGGGTGGCC 
AGTCAGTGGG AAAAGGCCTT CTGTGCTGAG TTGCAGCACT TTACAGAGTT GCACCCAAAG 
TTGGGCCTCT ACCCTTCTAC TTCATCTTCT CTGAGGACAG ACTTCCAGTT CTCCTCAGTG 
CTGGCACGTC GCCCCCTGTT GGCCAGCCTG GGAGTGTGTG GGGTGCTTGT CGTCCTTTGC 
TGCTCCATGA GGGACTGTGT GAGGTCCAAA CCCTGGCTGG GACTGCTAGC TCTGCTGTCC 
ATCACTCTAT CAGGCCTTAC TGCTGCTGGG ATACTCAACC TGACTGGGGC CACCTACAAC 
TCCACGTACT TGGGGATCCC GTTCGTCATG CTTGGTCATG GGCTGTTCGG CTCCTTCGAG 
ATGCTATCAT CGTGGAGACG AACCCGTGAG GACCAGCATG TGAAAGAGCG TGTTGCAAGT 
GTCTTCGAGG ATGTTATGTT GCGTTTCTCT GGCTCCACCA TGATCCACCT GATGACCTTG 
GGCCTGGCCG CCTCCCCGCT CACCAACATG GAAGCCGTCC GGCTCTTCTG CCGCACCGCT 
GCTCTGGCCG TTACCATCAG CTATGTTTAC ATGTTGTCCT TCTACAGTTC CTGTCTGGTG 
TTTACAGGCT ACCTGGAGAC CGGGTACAGA CATGGCTGCT TCTGCCGGCG AGTCCCCAAA 
CCGGACCGTT TGGACTCTAA ACCGGCTTGG TACAGGTGTT TAATGTACAC TCGTTACCAG 
GAGGAGACCC AAGTGACGAA TCCTCCACAC GGGATTGGTC ACACTCACAC ACCAAACCCT 
GACCTTACTC ACACGCACAC GCACCCTCAC CCGCATACAA TGAACAACCC AGCTGTTCGT 
GGACATGCTC ATGTTGCTAA CTCCACTCAC CCACACCCAC ACCCTCACCC ACACCCTCAC 
CCACATTCCA CAGCAAGCAT GGACCCTCAT CCTCAGGACT CACACCTTTT GTTGGGCTGT 
GTGCGGCGTT GCTATGGAGA CTGGATCACT AACACTTATG TTAAACCCTT TGTGGTTCTG 
CTGTATCTGG TTTACATCTC ATTTGGATTG ATGGGATTCC TACAGGTGAC CCAGGGTTCT 
GACCCCAGCG CACTGGTTGC AATGGACACC GCAACAGTAT TGTACACCCG AGCCCAGCAG 
CGTTACTTCA GCTCATACTC TCCTGTGATT GGATTCTACA TTTATGAAAG TGCCCCTTAC 
TGGAATGCCT CAGTGCAGCG GGATCTGCTG GAATATGCCA AAGGCTTCCA GAGAATAAGC 
TGGCTGGAAG CTTACCTGAA CTACCTGTCG GATCGCAACC AGTCCACCAG CCAGCCGCGG 
GAAAATTTCA CCCGCACGCT ACGCCACGCC TTTCTCCGTG AGCCGCAGTT TGCTCACTTT 
GCGGATGACA TTATATTTGC AGAGCGCGGC CAGGGCGAAG AGCCTGATGT GGCAGCTTCT 
CGCATATTCT TGGTTGCCAA AACAACGGAG AACAAGCGCG AGGAGATGTC AGTGCTGCTG 
GACACGCTGC GACGCCTGTC ACTGACCTCA CGAGTCCGCT TCTTAATCTT TAACCCTTCT 
TTCGTCTACC TGGACCGCTA TGCTGCGGCG GTCAGCTCCC CACTTAGACA CTCACTGCTG 
GCGGTGCTCT TCCTGTTGGG TCTCTCCTCT CTGGCTGTTG TGGAGCCCCT GGTGTCAGTG 
TGGCTGGGCC TCACCCTGCT CTCTGTCCAG TTTGGCGTCC TGGGCTTCAT GACCTTATGG 
GGTGTAGAGC TGGATTGCAT GTCTGTGCTA TGTTTGATCT CAGCTTTGGG GCACTCAGCA 
GACTGCAGCG GCCCTCTCCT CTGTGGCTTT GCTGCAGGTC GGGGTGAAAG CAGGACTCGC 
TGGGTGAGAG TGGCACTGGA GAGACACGGG GTTCCCTCTC TGCAGACTCT CATCTGTTAC 
GGCGCTGCCC TGGTACCCGT TGGCTCCATA CGCTCCAACC TCACACGCAC ACTATTTCGC 
TGCCTCACCC TAACAGCTGG TTGCTCGGCC CTGCACACGC TGGCATTCCT GCCCACACTC 
CTCACCTTCC TGCCCCCCTC CAAGAGCCGG GGCCATGGAC CTGGAGGGGA AGGACAGAGA 
CAGGAGGTGG AGTGTGTTGA AATGAATGAC AGCACCAGAG TTGTCGACCA GATCACCACC 
GTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005449141.1
CDS	296..2821
Translation	MQVTVPATGFNSFNYSFSNLCLLDDKNVCIIDDIIRAMEEIQSARNSNRSVPVLRYPITQLADGRQAYIGHQMGGVQGWGPGGAMRVQGMGVRGEGVRSARALQLTYYLQAPGGLMDRVASQWEKAFCAELQHFTELHPKLGLYPSTSSSLRTDFQFSSVLARRPLLASLGVCGVLVVLCCSMRDCVRSKPWLGLLALLSITLSGLTAAGILNLTGATYNSTYLGIPFVMLGHGLFGSFEMLSSWRRTREDQHVKERVASVFEDVMLRFSGSTMIHLMTLGLAASPLTNMEAVRLFCRTAALAVTISYVYMLSFYSSCLVFTGYLETGYRHGCFCRRVPKPDRLDSKPAWYRCLMYTRYQEETQVTNPPHGIGHTHTPNPDLTHTHTHPHPHTMNNPAVRGHAHVANSTHPHPHPHPHPHPHSTASMDPHPQDSHLLLGCVRRCYGDWITNTYVKPFVVLLYLVYISFGLMGFLQVTQGSDPSALVAMDTATVLYTRAQQRYFSSYSPVIGFYIYESAPYWNASVQRDLLEYAKGFQRISWLEAYLNYLSDRNQSTSQPRENFTRTLRHAFLREPQFAHFADDIIFAERGQGEEPDVAASRIFLVAKTTENKREEMSVLLDTLRRLSLTSRVRFLIFNPSFVYLDRYAAAVSSPLRHSLLAVLFLLGLSSLAVVEPLVSVWLGLTLLSVQFGVLGFMTLWGVELDCMSVLCLISALGHSADCSGPLLCGFAAGRGESRTRWVRVALERHGVPSLQTLICYGAALVPVGSIRSNLTRTLFRCLTLTAGCSALHTLAFLPTLLTFLPPSKSRGHGPGGEGQRQEVECVEMNDSTRVVDQITTV

Target ORF information:

RefSeq Version	XM_005449084.3
Organism	Oreochromis niloticus(Nile tilapia)
Definition	Oreochromis niloticus patched domain containing 1 (ptchd1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005449084.3

ORF Insert Sequence:

ATGCAGGTAA CCGTGCCAGC AACAGGCTTC AACAGTTTCA ACTACTCCTT CTCCAACCTT 
TGCCTTCTTG ATGACAAGAA TGTCTGCATC ATCGATGACA TCATCCGCGC CATGGAGGAG 
ATCCAGTCAG CTCGCAACTC CAATCGCTCC GTGCCCGTCC TGAGGTACCC CATCACACAG 
CTGGCAGATG GGCGGCAAGC GTACATTGGC CACCAGATGG GTGGTGTGCA GGGCTGGGGT 
CCTGGGGGGG CGATGCGAGT TCAGGGAATG GGAGTGAGGG GAGAAGGTGT CCGATCTGCC 
CGGGCGCTGC AGCTCACCTA CTACCTCCAA GCCCCAGGCG GCCTGATGGA CCGGGTGGCC 
AGTCAGTGGG AAAAGGCCTT CTGTGCTGAG TTGCAGCACT TTACAGAGTT GCACCCAAAG 
TTGGGCCTCT ACCCTTCTAC TTCATCTTCT CTGAGGACAG ACTTCCAGTT CTCCTCAGTG 
CTGGCACGTC GCCCCCTGTT GGCCAGCCTG GGAGTGTGTG GGGTGCTTGT CGTCCTTTGC 
TGCTCCATGA GGGACTGTGT GAGGTCCAAA CCCTGGCTGG GACTGCTAGC TCTGCTGTCC 
ATCACTCTAT CAGGCCTTAC TGCTGCTGGG ATACTCAACC TGACTGGGGC CACCTACAAC 
TCCACGTACT TGGGGATCCC GTTCGTCATG CTTGGTCATG GGCTGTTCGG CTCCTTCGAG 
ATGCTATCAT CGTGGAGACG AACCCGTGAG GACCAGCATG TGAAAGAGCG TGTTGCAAGT 
GTCTTCGAGG ATGTTATGTT GCGTTTCTCT GGCTCCACCA TGATCCACCT GATGACCTTG 
GGCCTGGCCG CCTCCCCGCT CACCAACATG GAAGCCGTCC GGCTCTTCTG CCGCACCGCT 
GCTCTGGCCG TTACCATCAG CTATGTTTAC ATGTTGTCCT TCTACAGTTC CTGTCTGGTG 
TTTACAGGCT ACCTGGAGAC CGGGTACAGA CATGGCTGCT TCTGCCGGCG AGTCCCCAAA 
CCGGACCGTT TGGACTCTAA ACCGGCTTGG TACAGGTGTT TAATGTACAC TCGTTACCAG 
GAGGAGACCC AAGTGACGAA TCCTCCACAC GGGATTGGTC ACACTCACAC ACCAAACCCT 
GACCTTACTC ACACGCACAC GCACCCTCAC CCGCATACAA TGAACAACCC AGCTGTTCGT 
GGACATGCTC ATGTTGCTAA CTCCACTCAC CCACACCCAC ACCCTCACCC ACACCCTCAC 
CCACATTCCA CAGCAAGCAT GGACCCTCAT CCTCAGGACT CACACCTTTT GTTGGGCTGT 
GTGCGGCGTT GCTATGGAGA CTGGATCACT AACACTTATG TTAAACCCTT TGTGGTTCTG 
CTGTATCTGG TTTACATCTC ATTTGGATTG ATGGGATTCC TACAGGTGAC CCAGGGTTCT 
GACCCCAGCG CACTGGTTGC AATGGACACC GCAACAGTAT TGTACACCCG AGCCCAGCAG 
CGTTACTTCA GCTCATACTC TCCTGTGATT GGATTCTACA TTTATGAAAG TGCCCCTTAC 
TGGAATGCCT CAGTGCAGCG GGATCTGCTG GAATATGCCA AAGGCTTCCA GAGAATAAGC 
TGGCTGGAAG CTTACCTGAA CTACCTGTCG GATCGCAACC AGTCCACCAG CCAGCCGCGG 
GAAAATTTCA CCCGCACGCT ACGCCACGCC TTTCTCCGTG AGCCGCAGTT TGCTCACTTT 
GCGGATGACA TTATATTTGC AGAGCGCGGC CAGGGCGAAG AGCCTGATGT GGCAGCTTCT 
CGCATATTCT TGGTTGCCAA AACAACGGAG AACAAGCGCG AGGAGATGTC AGTGCTGCTG 
GACACGCTGC GACGCCTGTC ACTGACCTCA CGAGTCCGCT TCTTAATCTT TAACCCTTCT 
TTCGTCTACC TGGACCGCTA TGCTGCGGCG GTCAGCTCCC CACTTAGACA CTCACTGCTG 
GCGGTGCTCT TCCTGTTGGG TCTCTCCTCT CTGGCTGTTG TGGAGCCCCT GGTGTCAGTG 
TGGCTGGGCC TCACCCTGCT CTCTGTCCAG TTTGGCGTCC TGGGCTTCAT GACCTTATGG 
GGTGTAGAGC TGGATTGCAT GTCTGTGCTA TGTTTGATCT CAGCTTTGGG GCACTCAGCA 
GACTGCAGCG GCCCTCTCCT CTGTGGCTTT GCTGCAGGTC GGGGTGAAAG CAGGACTCGC 
TGGGTGAGAG TGGCACTGGA GAGACACGGG GTTCCCTCTC TGCAGACTCT CATCTGTTAC 
GGCGCTGCCC TGGTACCCGT TGGCTCCATA CGCTCCAACC TCACACGCAC ACTATTTCGC 
TGCCTCACCC TAACAGCTGG TTGCTCGGCC CTGCACACGC TGGCATTCCT GCCCACACTC 
CTCACCTTCC TGCCCCCCTC CAAGAGCCGG GGCCATGGAC CTGGAGGGGA AGGACAGAGA 
CAGGAGGTGG AGTGTGTTGA AATGAATGAC AGCACCAGAG TTGTCGACCA GATCACCACC 
GTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ptchd1 ( XM_005449084.4 ) cDNA ORF clone, Oreochromis niloticus(Nile tilapia) -> XP_005449141.1 Oreochromis niloticus patched domain containing 1 (ptchd1), transcript variant X4, mRNA.

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CloneID	OOr71680
Clone ID Related Accession (Same CDS sequence)	XM_005449084.3 , XM_005449084.4
Accession Version	XM_005449084.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2526bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-07-25
Organism	Oreochromis niloticus(Nile tilapia)
Product	patched domain-containing protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_031974.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2018 this sequence version replaced XM_005449084.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Oreochromis niloticus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGGTAA CCGTGCCAGC AACAGGCTTC AACAGTTTCA ACTACTCCTT CTCCAACCTT 
TGCCTTCTTG ATGACAAGAA TGTCTGCATC ATCGATGACA TCATCCGCGC CATGGAGGAG 
ATCCAGTCAG CTCGCAACTC CAATCGCTCC GTGCCCGTCC TGAGGTACCC CATCACACAG 
CTGGCAGATG GGCGGCAAGC GTACATTGGC CACCAGATGG GTGGTGTGCA GGGCTGGGGT 
CCTGGGGGGG CGATGCGAGT TCAGGGAATG GGAGTGAGGG GAGAAGGTGT CCGATCTGCC 
CGGGCGCTGC AGCTCACCTA CTACCTCCAA GCCCCAGGCG GCCTGATGGA CCGGGTGGCC 
AGTCAGTGGG AAAAGGCCTT CTGTGCTGAG TTGCAGCACT TTACAGAGTT GCACCCAAAG 
TTGGGCCTCT ACCCTTCTAC TTCATCTTCT CTGAGGACAG ACTTCCAGTT CTCCTCAGTG 
CTGGCACGTC GCCCCCTGTT GGCCAGCCTG GGAGTGTGTG GGGTGCTTGT CGTCCTTTGC 
TGCTCCATGA GGGACTGTGT GAGGTCCAAA CCCTGGCTGG GACTGCTAGC TCTGCTGTCC 
ATCACTCTAT CAGGCCTTAC TGCTGCTGGG ATACTCAACC TGACTGGGGC CACCTACAAC 
TCCACGTACT TGGGGATCCC GTTCGTCATG CTTGGTCATG GGCTGTTCGG CTCCTTCGAG 
ATGCTATCAT CGTGGAGACG AACCCGTGAG GACCAGCATG TGAAAGAGCG TGTTGCAAGT 
GTCTTCGAGG ATGTTATGTT GCGTTTCTCT GGCTCCACCA TGATCCACCT GATGACCTTG 
GGCCTGGCCG CCTCCCCGCT CACCAACATG GAAGCCGTCC GGCTCTTCTG CCGCACCGCT 
GCTCTGGCCG TTACCATCAG CTATGTTTAC ATGTTGTCCT TCTACAGTTC CTGTCTGGTG 
TTTACAGGCT ACCTGGAGAC CGGGTACAGA CATGGCTGCT TCTGCCGGCG AGTCCCCAAA 
CCGGACCGTT TGGACTCTAA ACCGGCTTGG TACAGGTGTT TAATGTACAC TCGTTACCAG 
GAGGAGACCC AAGTGACGAA TCCTCCACAC GGGATTGGTC ACACTCACAC ACCAAACCCT 
GACCTTACTC ACACGCACAC GCACCCTCAC CCGCATACAA TGAACAACCC AGCTGTTCGT 
GGACATGCTC ATGTTGCTAA CTCCACTCAC CCACACCCAC ACCCTCACCC ACACCCTCAC 
CCACATTCCA CAGCAAGCAT GGACCCTCAT CCTCAGGACT CACACCTTTT GTTGGGCTGT 
GTGCGGCGTT GCTATGGAGA CTGGATCACT AACACTTATG TTAAACCCTT TGTGGTTCTG 
CTGTATCTGG TTTACATCTC ATTTGGATTG ATGGGATTCC TACAGGTGAC CCAGGGTTCT 
GACCCCAGCG CACTGGTTGC AATGGACACC GCAACAGTAT TGTACACCCG AGCCCAGCAG 
CGTTACTTCA GCTCATACTC TCCTGTGATT GGATTCTACA TTTATGAAAG TGCCCCTTAC 
TGGAATGCCT CAGTGCAGCG GGATCTGCTG GAATATGCCA AAGGCTTCCA GAGAATAAGC 
TGGCTGGAAG CTTACCTGAA CTACCTGTCG GATCGCAACC AGTCCACCAG CCAGCCGCGG 
GAAAATTTCA CCCGCACGCT ACGCCACGCC TTTCTCCGTG AGCCGCAGTT TGCTCACTTT 
GCGGATGACA TTATATTTGC AGAGCGCGGC CAGGGCGAAG AGCCTGATGT GGCAGCTTCT 
CGCATATTCT TGGTTGCCAA AACAACGGAG AACAAGCGCG AGGAGATGTC AGTGCTGCTG 
GACACGCTGC GACGCCTGTC ACTGACCTCA CGAGTCCGCT TCTTAATCTT TAACCCTTCT 
TTCGTCTACC TGGACCGCTA TGCTGCGGCG GTCAGCTCCC CACTTAGACA CTCACTGCTG 
GCGGTGCTCT TCCTGTTGGG TCTCTCCTCT CTGGCTGTTG TGGAGCCCCT GGTGTCAGTG 
TGGCTGGGCC TCACCCTGCT CTCTGTCCAG TTTGGCGTCC TGGGCTTCAT GACCTTATGG 
GGTGTAGAGC TGGATTGCAT GTCTGTGCTA TGTTTGATCT CAGCTTTGGG GCACTCAGCA 
GACTGCAGCG GCCCTCTCCT CTGTGGCTTT GCTGCAGGTC GGGGTGAAAG CAGGACTCGC 
TGGGTGAGAG TGGCACTGGA GAGACACGGG GTTCCCTCTC TGCAGACTCT CATCTGTTAC 
GGCGCTGCCC TGGTACCCGT TGGCTCCATA CGCTCCAACC TCACACGCAC ACTATTTCGC 
TGCCTCACCC TAACAGCTGG TTGCTCGGCC CTGCACACGC TGGCATTCCT GCCCACACTC 
CTCACCTTCC TGCCCCCCTC CAAGAGCCGG GGCCATGGAC CTGGAGGGGA AGGACAGAGA 
CAGGAGGTGG AGTGTGTTGA AATGAATGAC AGCACCAGAG TTGTCGACCA GATCACCACC 
GTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005449141.1
CDS	274..2799
Translation	MQVTVPATGFNSFNYSFSNLCLLDDKNVCIIDDIIRAMEEIQSARNSNRSVPVLRYPITQLADGRQAYIGHQMGGVQGWGPGGAMRVQGMGVRGEGVRSARALQLTYYLQAPGGLMDRVASQWEKAFCAELQHFTELHPKLGLYPSTSSSLRTDFQFSSVLARRPLLASLGVCGVLVVLCCSMRDCVRSKPWLGLLALLSITLSGLTAAGILNLTGATYNSTYLGIPFVMLGHGLFGSFEMLSSWRRTREDQHVKERVASVFEDVMLRFSGSTMIHLMTLGLAASPLTNMEAVRLFCRTAALAVTISYVYMLSFYSSCLVFTGYLETGYRHGCFCRRVPKPDRLDSKPAWYRCLMYTRYQEETQVTNPPHGIGHTHTPNPDLTHTHTHPHPHTMNNPAVRGHAHVANSTHPHPHPHPHPHPHSTASMDPHPQDSHLLLGCVRRCYGDWITNTYVKPFVVLLYLVYISFGLMGFLQVTQGSDPSALVAMDTATVLYTRAQQRYFSSYSPVIGFYIYESAPYWNASVQRDLLEYAKGFQRISWLEAYLNYLSDRNQSTSQPRENFTRTLRHAFLREPQFAHFADDIIFAERGQGEEPDVAASRIFLVAKTTENKREEMSVLLDTLRRLSLTSRVRFLIFNPSFVYLDRYAAAVSSPLRHSLLAVLFLLGLSSLAVVEPLVSVWLGLTLLSVQFGVLGFMTLWGVELDCMSVLCLISALGHSADCSGPLLCGFAAGRGESRTRWVRVALERHGVPSLQTLICYGAALVPVGSIRSNLTRTLFRCLTLTAGCSALHTLAFLPTLLTFLPPSKSRGHGPGGEGQRQEVECVEMNDSTRVVDQITTV

Target ORF information:

RefSeq Version	XM_005449084.4
Organism	Oreochromis niloticus(Nile tilapia)
Definition	Oreochromis niloticus patched domain containing 1 (ptchd1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005449084.4

ORF Insert Sequence:

ATGCAGGTAA CCGTGCCAGC AACAGGCTTC AACAGTTTCA ACTACTCCTT CTCCAACCTT 
TGCCTTCTTG ATGACAAGAA TGTCTGCATC ATCGATGACA TCATCCGCGC CATGGAGGAG 
ATCCAGTCAG CTCGCAACTC CAATCGCTCC GTGCCCGTCC TGAGGTACCC CATCACACAG 
CTGGCAGATG GGCGGCAAGC GTACATTGGC CACCAGATGG GTGGTGTGCA GGGCTGGGGT 
CCTGGGGGGG CGATGCGAGT TCAGGGAATG GGAGTGAGGG GAGAAGGTGT CCGATCTGCC 
CGGGCGCTGC AGCTCACCTA CTACCTCCAA GCCCCAGGCG GCCTGATGGA CCGGGTGGCC 
AGTCAGTGGG AAAAGGCCTT CTGTGCTGAG TTGCAGCACT TTACAGAGTT GCACCCAAAG 
TTGGGCCTCT ACCCTTCTAC TTCATCTTCT CTGAGGACAG ACTTCCAGTT CTCCTCAGTG 
CTGGCACGTC GCCCCCTGTT GGCCAGCCTG GGAGTGTGTG GGGTGCTTGT CGTCCTTTGC 
TGCTCCATGA GGGACTGTGT GAGGTCCAAA CCCTGGCTGG GACTGCTAGC TCTGCTGTCC 
ATCACTCTAT CAGGCCTTAC TGCTGCTGGG ATACTCAACC TGACTGGGGC CACCTACAAC 
TCCACGTACT TGGGGATCCC GTTCGTCATG CTTGGTCATG GGCTGTTCGG CTCCTTCGAG 
ATGCTATCAT CGTGGAGACG AACCCGTGAG GACCAGCATG TGAAAGAGCG TGTTGCAAGT 
GTCTTCGAGG ATGTTATGTT GCGTTTCTCT GGCTCCACCA TGATCCACCT GATGACCTTG 
GGCCTGGCCG CCTCCCCGCT CACCAACATG GAAGCCGTCC GGCTCTTCTG CCGCACCGCT 
GCTCTGGCCG TTACCATCAG CTATGTTTAC ATGTTGTCCT TCTACAGTTC CTGTCTGGTG 
TTTACAGGCT ACCTGGAGAC CGGGTACAGA CATGGCTGCT TCTGCCGGCG AGTCCCCAAA 
CCGGACCGTT TGGACTCTAA ACCGGCTTGG TACAGGTGTT TAATGTACAC TCGTTACCAG 
GAGGAGACCC AAGTGACGAA TCCTCCACAC GGGATTGGTC ACACTCACAC ACCAAACCCT 
GACCTTACTC ACACGCACAC GCACCCTCAC CCGCATACAA TGAACAACCC AGCTGTTCGT 
GGACATGCTC ATGTTGCTAA CTCCACTCAC CCACACCCAC ACCCTCACCC ACACCCTCAC 
CCACATTCCA CAGCAAGCAT GGACCCTCAT CCTCAGGACT CACACCTTTT GTTGGGCTGT 
GTGCGGCGTT GCTATGGAGA CTGGATCACT AACACTTATG TTAAACCCTT TGTGGTTCTG 
CTGTATCTGG TTTACATCTC ATTTGGATTG ATGGGATTCC TACAGGTGAC CCAGGGTTCT 
GACCCCAGCG CACTGGTTGC AATGGACACC GCAACAGTAT TGTACACCCG AGCCCAGCAG 
CGTTACTTCA GCTCATACTC TCCTGTGATT GGATTCTACA TTTATGAAAG TGCCCCTTAC 
TGGAATGCCT CAGTGCAGCG GGATCTGCTG GAATATGCCA AAGGCTTCCA GAGAATAAGC 
TGGCTGGAAG CTTACCTGAA CTACCTGTCG GATCGCAACC AGTCCACCAG CCAGCCGCGG 
GAAAATTTCA CCCGCACGCT ACGCCACGCC TTTCTCCGTG AGCCGCAGTT TGCTCACTTT 
GCGGATGACA TTATATTTGC AGAGCGCGGC CAGGGCGAAG AGCCTGATGT GGCAGCTTCT 
CGCATATTCT TGGTTGCCAA AACAACGGAG AACAAGCGCG AGGAGATGTC AGTGCTGCTG 
GACACGCTGC GACGCCTGTC ACTGACCTCA CGAGTCCGCT TCTTAATCTT TAACCCTTCT 
TTCGTCTACC TGGACCGCTA TGCTGCGGCG GTCAGCTCCC CACTTAGACA CTCACTGCTG 
GCGGTGCTCT TCCTGTTGGG TCTCTCCTCT CTGGCTGTTG TGGAGCCCCT GGTGTCAGTG 
TGGCTGGGCC TCACCCTGCT CTCTGTCCAG TTTGGCGTCC TGGGCTTCAT GACCTTATGG 
GGTGTAGAGC TGGATTGCAT GTCTGTGCTA TGTTTGATCT CAGCTTTGGG GCACTCAGCA 
GACTGCAGCG GCCCTCTCCT CTGTGGCTTT GCTGCAGGTC GGGGTGAAAG CAGGACTCGC 
TGGGTGAGAG TGGCACTGGA GAGACACGGG GTTCCCTCTC TGCAGACTCT CATCTGTTAC 
GGCGCTGCCC TGGTACCCGT TGGCTCCATA CGCTCCAACC TCACACGCAC ACTATTTCGC 
TGCCTCACCC TAACAGCTGG TTGCTCGGCC CTGCACACGC TGGCATTCCT GCCCACACTC 
CTCACCTTCC TGCCCCCCTC CAAGAGCCGG GGCCATGGAC CTGGAGGGGA AGGACAGAGA 
CAGGAGGTGG AGTGTGTTGA AATGAATGAC AGCACCAGAG TTGTCGACCA GATCACCACC 
GTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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ptchd1 cDNA ORF clone, Oreochromis niloticus(Nile tilapia)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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見積依頼

Eメール

電話

FAX

オンラインフォーム