csf1-2 cDNA ORF clone, Oncorhynchus mykiss(rainbow trout)

Gene
Clones
gRNAs

Summary

Gene Symbol	csf1-2
Entrez Gene ID	100301638
Full Name	precursor of second macrophage colony-stimulating factor
Gene Type	protein-coding
Organism	Oncorhynchus mykiss(rainbow trout)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001160476.1	NP_001153948.1	second macrophage colony-stimulating factor precursor

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

Identification of IL-34 in teleost fish: differential expression of rainbow trout IL-34, MCSF1 and MCSF2, ligands of the MCSF receptor.
Wang T, Kono T, Monte MM, Kuse H, Costa MM, Korenaga H, Maehr T, Husain M, Sakai M, Secombes CJ
Molecular immunology53(4)398-409(2013 Apr)

Two macrophage colony-stimulating factor genes exist in fish that differ in gene organization and are differentially expressed.
Wang T, Hanington PC, Belosevic M, Secombes CJ
Journal of immunology (Baltimore, Md. : 1950)181(5)3310-22(2008 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The expression of MCSF2 showed great variance in different tissues and cell lines, suggesting a role in the differentiation and maintenance of specific macrophage lineages in specific locations.
Title: Identification of IL-34 in teleost fish: differential expression of rainbow trout IL-34, MCSF1 and MCSF2, ligands of the MCSF receptor.

The following csf1-2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the csf1-2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OOf00490	NM_001160476.1 Latest version!	Oncorhynchus mykiss precursor of second macrophage colony-stimulating factor (csf1-2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

csf1-2 ( NM_001160476.1 ) cDNA ORF clone, Oncorhynchus mykiss(rainbow trout) -> NP_001153948.1 Oncorhynchus mykiss precursor of second macrophage colony-stimulating factor (csf1-2), mRNA.

Price & Availability↑

CloneID	OOf00490
Clone ID Related Accession (Same CDS sequence)	NM_001160476.1
Accession Version	NM_001160476.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 831bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-08-05
Organism	Oncorhynchus mykiss(rainbow trout)
Product	second macrophage colony-stimulating factor precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AM949839.1. ##Evidence-Data-START## Transcript exon combination :: AM949839.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00041048, SAMD00041049 [ECO:0000348] ##Evidence-Data-END##

ATGACCCTCC TCGTGCCAAC CCACATTCAG TGCAAAACCA AGGTAAAGTG TCTGTGTGTG 
ATGGTGTTCT TGAGTCTCCC CTTGACCATG GGAGACCCTG GTCCGTGCAG ACACTCCATC 
ACCAGAGATC ACCTGCTGAC CATAAGACAC CTGATAGATA ACCAGTTGCA GAGTGGATGC 
TCAATAACCT ACACATTTAT TGAGCAGAGA AGTTTGAATA AATGTTGCTA TGTAAAAGCT 
GCCATGCCCT GGATCTTGGA ACTCCTGACC GGACACTTCA GATACACTCG GGGATCAGAC 
AACCACAGCT ACGTGTTGTC GCTGACCGGT CTCATCCACA ACATTTACTC CCAGAGATGT 
GTACCACAGA TCAATGAGGA GCTGGAGGAC GACCCAGTGA GTTTTGGAAT GTCATTCAGC 
AGTTCTCCAT CTGAGGCCCT GGGGAGGGTC CAGGATGTCC TGTCTGTGTA CTGGGAGCTG 
GTGACCACCA TGGACTCACC AGTAGACTGG AGCTGTGAGA GGGAGTACAC AGACACCGCA 
GAGCCTCCCA CAGCCCTCCC TACAGCACTC TCTCTGACTA CAGTCAGCAC CCTTTGGGAC 
TCAGAGAAAG CCCCTCAGAC TAGTCTAGAT GGAGACCAAG ATAGAGACCT GTACAAGTTT 
GGTTTCATGG TCATAGCTCC ATCAGTCTGT GGAGGACTGC TTTTCATATT CACTCTCTAT 
TGCCTCATCA CACACAAGAT GTCCTACAGT ACTGACCATC ACACATCACT AGGGTACCAA 
AATTCAAGTT TGCATACAGA TATTCAGGAC ATAGAGATGC AGGTGGAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001153948.1
CDS	51..881
Translation	MTLLVPTHIQCKTKVKCLCVMVFLSLPLTMGDPGPCRHSITRDHLLTIRHLIDNQLQSGCSITYTFIEQRSLNKCCYVKAAMPWILELLTGHFRYTRGSDNHSYVLSLTGLIHNIYSQRCVPQINEELEDDPVSFGMSFSSSPSEALGRVQDVLSVYWELVTTMDSPVDWSCEREYTDTAEPPTALPTALSLTTVSTLWDSEKAPQTSLDGDQDRDLYKFGFMVIAPSVCGGLLFIFTLYCLITHKMSYSTDHHTSLGYQNSSLHTDIQDIEMQVE

Target ORF information:

RefSeq Version	NM_001160476.1
Organism	Oncorhynchus mykiss(rainbow trout)
Definition	Oncorhynchus mykiss precursor of second macrophage colony-stimulating factor (csf1-2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001160476.1

ORF Insert Sequence:

ATGACCCTCC TCGTGCCAAC CCACATTCAG TGCAAAACCA AGGTAAAGTG TCTGTGTGTG 
ATGGTGTTCT TGAGTCTCCC CTTGACCATG GGAGACCCTG GTCCGTGCAG ACACTCCATC 
ACCAGAGATC ACCTGCTGAC CATAAGACAC CTGATAGATA ACCAGTTGCA GAGTGGATGC 
TCAATAACCT ACACATTTAT TGAGCAGAGA AGTTTGAATA AATGTTGCTA TGTAAAAGCT 
GCCATGCCCT GGATCTTGGA ACTCCTGACC GGACACTTCA GATACACTCG GGGATCAGAC 
AACCACAGCT ACGTGTTGTC GCTGACCGGT CTCATCCACA ACATTTACTC CCAGAGATGT 
GTACCACAGA TCAATGAGGA GCTGGAGGAC GACCCAGTGA GTTTTGGAAT GTCATTCAGC 
AGTTCTCCAT CTGAGGCCCT GGGGAGGGTC CAGGATGTCC TGTCTGTGTA CTGGGAGCTG 
GTGACCACCA TGGACTCACC AGTAGACTGG AGCTGTGAGA GGGAGTACAC AGACACCGCA 
GAGCCTCCCA CAGCCCTCCC TACAGCACTC TCTCTGACTA CAGTCAGCAC CCTTTGGGAC 
TCAGAGAAAG CCCCTCAGAC TAGTCTAGAT GGAGACCAAG ATAGAGACCT GTACAAGTTT 
GGTTTCATGG TCATAGCTCC ATCAGTCTGT GGAGGACTGC TTTTCATATT CACTCTCTAT 
TGCCTCATCA CACACAAGAT GTCCTACAGT ACTGACCATC ACACATCACT AGGGTACCAA 
AATTCAAGTT TGCATACAGA TATTCAGGAC ATAGAGATGC AGGTGGAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

Identification of IL-34 in teleost fish: differential expression of rainbow trout IL-34, MCSF1 and MCSF2, ligands of the MCSF receptor.
Molecular immunology53(4)398-409(2013 Apr)
Wang T,Kono T,Monte MM,Kuse H,Costa MM,Korenaga H,Maehr T,Husain M,Sakai M,Secombes CJ

Two macrophage colony-stimulating factor genes exist in fish that differ in gene organization and are differentially expressed.
Journal of immunology (Baltimore, Md. : 1950)181(5)3310-22(2008 Sep)
Wang T,Hanington PC,Belosevic M,Secombes CJ