mthfd2 cDNA ORF clone, Nothobranchius furzeri(turquoise killifish)

Gene
Clones
gRNAs

Summary

Gene Symbol	mthfd2
Entrez Gene ID	107394218
Full Name	methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2, methenyltetrahydrofolate cyclohydrolase
Gene Type	protein-coding
Organism	Nothobranchius furzeri(turquoise killifish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015973073.1	XP_015828559.1	bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, mitochondrial

Pathways from BioSystems
Gene Ontology

The following mthfd2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the mthfd2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ONo27783	XM_015973073.1 Latest version!	Nothobranchius furzeri methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2, methenyltetrahydrofolate cyclohydrolase (mthfd2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

mthfd2 ( XM_015973073.1 ) cDNA ORF clone, Nothobranchius furzeri(turquoise killifish) -> XP_015828559.1 Nothobranchius furzeri methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2, methenyltetrahydrofolate cyclohydrolase (mthfd2), mRNA.

Price & Availability↑

CloneID	ONo27783
Clone ID Related Accession (Same CDS sequence)	XM_015973073.1
Accession Version	XM_015973073.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1017bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-03-17
Organism	Nothobranchius furzeri(turquoise killifish)
Product	bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, mitochondrial
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015443601.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Nothobranchius furzeri Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGA TCAGGACTCT CGGGAAACTG TGTCAACAGT CTCAGCATCA AGTCTGTAAA 
CTGCACACAT CCGCCTTGAG GCAGGACGCA GTGGTCGTCT CAGGAAGGAA ACTGGCACGC 
CAGATTCGGG AGGAGACCCA GGTCGATGTG GAGAAATGGG TTTTAGCCGG CAACAGGCGA 
CCTCACCTGA GTGTGGTTCT TGTTGGAGAC GACCCAGCCA GTCACTCCTA CGTTCTCAAC 
AAGACCCGGG CAGCAGCTGA TGTCGGAATC TCAAGTGAGA CGATTCTCAA ACACTCAGAC 
ATCAGTGAGG AGGAGTTACT GGACTTGATC TACAAACTCA ATGCAGACCA TCGTGTGGAC 
GGCCTGCTGG TCCAGCTGCC TCTGCCAGAT CACATTGATG AGCGGGTAAT CTGCAATGCG 
GTTGCCCCTG CCAAGGATGT AGACGGCTTC CACGTGGTTA ACGTTGGCCG CATGTGCCTG 
GATCAGTCCA CCATGCTTCC AGCCACCCCC TGGGGAGTCT GGGAAATCAT CAAACGCACA 
GGTATTCCCA CTTTTGGGAA GAATGTATTA GTTGCAGGAC GCTCTAAGAA CGTGGGAATG 
CCCATCGCCA TGTTACTGCA CACAGACGGA CGCCACGAGA GGCCTGGAGG TGATGCCACA 
GTCACCATTT CTCACCGTTA CACTCCAAAG GAACAGCTTT GTCAGCACAC AAAAATGGCA 
GACATCATCG TGGCCGCTGC AGGGATCCCA AACCTGATCA CAGCAGACAT GATCAAAGAG 
GGGGCAGCTG TGATTGACGT CGGAATAAAC CGAATCCAGG ACCCCGTCAC TGGAAAAAGC 
CGCCTGGTTG GAGATGTGGA CTTTGAAGGC GTAAAGAAGA AGGCTGGATT CATCACACCA 
GTTCCTGGAG GTGTTGGGCC CATGACTGTG GCCATGCTCA TGAAGAACAC CATCAAAGCT 
GCCAAGAATG TCCTGCAGAT TCCCGCAGAG AGGATCCGGA TGGCTGCTGC ATCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015828559.1
CDS	129..1145
Translation	MAAIRTLGKLCQQSQHQVCKLHTSALRQDAVVVSGRKLARQIREETQVDVEKWVLAGNRRPHLSVVLVGDDPASHSYVLNKTRAAADVGISSETILKHSDISEEELLDLIYKLNADHRVDGLLVQLPLPDHIDERVICNAVAPAKDVDGFHVVNVGRMCLDQSTMLPATPWGVWEIIKRTGIPTFGKNVLVAGRSKNVGMPIAMLLHTDGRHERPGGDATVTISHRYTPKEQLCQHTKMADIIVAAAGIPNLITADMIKEGAAVIDVGINRIQDPVTGKSRLVGDVDFEGVKKKAGFITPVPGGVGPMTVAMLMKNTIKAAKNVLQIPAERIRMAAAS

Target ORF information:

RefSeq Version	XM_015973073.1
Organism	Nothobranchius furzeri(turquoise killifish)
Definition	Nothobranchius furzeri methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2, methenyltetrahydrofolate cyclohydrolase (mthfd2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015973073.1

ORF Insert Sequence:

ATGGCGGCGA TCAGGACTCT CGGGAAACTG TGTCAACAGT CTCAGCATCA AGTCTGTAAA 
CTGCACACAT CCGCCTTGAG GCAGGACGCA GTGGTCGTCT CAGGAAGGAA ACTGGCACGC 
CAGATTCGGG AGGAGACCCA GGTCGATGTG GAGAAATGGG TTTTAGCCGG CAACAGGCGA 
CCTCACCTGA GTGTGGTTCT TGTTGGAGAC GACCCAGCCA GTCACTCCTA CGTTCTCAAC 
AAGACCCGGG CAGCAGCTGA TGTCGGAATC TCAAGTGAGA CGATTCTCAA ACACTCAGAC 
ATCAGTGAGG AGGAGTTACT GGACTTGATC TACAAACTCA ATGCAGACCA TCGTGTGGAC 
GGCCTGCTGG TCCAGCTGCC TCTGCCAGAT CACATTGATG AGCGGGTAAT CTGCAATGCG 
GTTGCCCCTG CCAAGGATGT AGACGGCTTC CACGTGGTTA ACGTTGGCCG CATGTGCCTG 
GATCAGTCCA CCATGCTTCC AGCCACCCCC TGGGGAGTCT GGGAAATCAT CAAACGCACA 
GGTATTCCCA CTTTTGGGAA GAATGTATTA GTTGCAGGAC GCTCTAAGAA CGTGGGAATG 
CCCATCGCCA TGTTACTGCA CACAGACGGA CGCCACGAGA GGCCTGGAGG TGATGCCACA 
GTCACCATTT CTCACCGTTA CACTCCAAAG GAACAGCTTT GTCAGCACAC AAAAATGGCA 
GACATCATCG TGGCCGCTGC AGGGATCCCA AACCTGATCA CAGCAGACAT GATCAAAGAG 
GGGGCAGCTG TGATTGACGT CGGAATAAAC CGAATCCAGG ACCCCGTCAC TGGAAAAAGC 
CGCCTGGTTG GAGATGTGGA CTTTGAAGGC GTAAAGAAGA AGGCTGGATT CATCACACCA 
GTTCCTGGAG GTGTTGGGCC CATGACTGTG GCCATGCTCA TGAAGAACAC CATCAAAGCT 
GCCAAGAATG TCCTGCAGAT TCCCGCAGAG AGGATCCGGA TGGCTGCTGC ATCCTAA