slc8a3 cDNA ORF clone, Nothobranchius furzeri(turquoise killifish)

Gene
Clones
gRNAs

Summary

Gene Symbol	slc8a3
Entrez Gene ID	107391736
Full Name	solute carrier family 8 member A3
Gene Type	protein-coding
Organism	Nothobranchius furzeri(turquoise killifish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015969169.1	XP_015824655.1	sodium/calcium exchanger 3 isoform X1
XM_015969170.1	XP_015824656.1	sodium/calcium exchanger 3 isoform X2

Pathways from BioSystems
Gene Ontology

The following slc8a3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the slc8a3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ONo24618	XM_015969169.1 Latest version!	Nothobranchius furzeri solute carrier family 8 member A3 (slc8a3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
ONo24619	XM_015969170.1 Latest version!	Nothobranchius furzeri solute carrier family 8 member A3 (slc8a3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

slc8a3 ( XM_015969169.1 ) cDNA ORF clone, Nothobranchius furzeri(turquoise killifish) -> XP_015824655.1 Nothobranchius furzeri solute carrier family 8 member A3 (slc8a3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ONo24618
Clone ID Related Accession (Same CDS sequence)	XM_015969169.1
Accession Version	XM_015969169.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2778bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-03-17
Organism	Nothobranchius furzeri(turquoise killifish)
Product	sodium/calcium exchanger 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015443591.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Nothobranchius furzeri Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGGTT CAAGGCCTGT AAAACCGGGG TCATCAGCCT GCCTCTGGTT GGGGCTGATC 
TCTGTGGCTG TTGCTTTCCT TTGTTCTGAG GCCCGGACCA CTCCCAGCCC TTTGCTAAGC 
ATCAACAATG CCACTTGCAA AGGAACCTCG AAGTGTCAAG CAGGGATCAT CCTTCCCATC 
TGGTATCCAG AGGATCCTTC CATGGGGGAC AAGATTGCTC GAGTCATTGT CTATTTTGTG 
GCCATGATCT ACATGTTTCT TGGTGTATCC ATCATTGCTG ACCGCTTCAT GGCAGCCATT 
GAGGTCATCA CCTCCCAGGA AAGAGAAATT GTTATCAAAA GGCCCAATGG GGAAACAACC 
ACAACCACAA TCCGGGTGTG GAACGAAACA GTCTCCAATC TTACCCTCAT GGCCTTGGGC 
TCCTCTGCTC CTGAGATTTT GCTCTCTCTG ATTGAGGTTT GTGGACATGA GTTTAAATCT 
GGTGAACTTG GCCCTTCCAC TATTGTTGGC AGTGCAGCTT TCAATATGTT TGTCATAATT 
GGCCTTTGTG TGTCTGTCAT TCCCCAAGGA GAAGTACGAA AAGTCAAGCA CCTCAGAGTG 
TTCTTTGTCA CAGCAGGGTG GAGTATCTTT GCCTACATCT GGCTTTACAT GATTCTGGCG 
GTGTTTTCTC CCAACGTAGT CCAGGTCTGG GAAGGTCTAG TCACACTGGC CTTCTTCCCC 
ATATGTGTTA TTCTTGCCTG GGTAGCAGAC AGACGACTGT TATTCTACAA GTTTATGCAC 
AAGAAGTATC GCACTGATAA ACACAGGGGC GTCATTATTG AGACTGAGAC CGAACGCTCC 
AAGGGAATTG AAATGGACGG CAAGATGGTC AACTCCCACT TCATGGATGG AGGTGCAGCC 
AGCAATCTTA TTGGTCTGAT TGAGAGCAAA GAAGTAGACG AATCTCGTCG AGACATGATT 
CGTATCTTAA AGGACCTAAA GCAGAAGTAC CCTGAGAAAG AGCTTGATCA ACTGGTTGAG 
ATGGCCAACT ACTATGCCCT CTCACACCAG CAAAAGAGCC GTGCCTTCTA CCGTATACAG 
GCCACACGCA TGATGACAGG CGCTGGAAAT ATCCTGAAGA AACACGTTGC AGAGCAGGCA 
AAGAAGAGCG CTAGTGTGCA GGAGGTGCAC GTGGATGAAC CAGAAGAGTT TGTGTCTCGG 
ATAGCTTTTG AGCCCGCTGT CTATCAGTGC CTTGAGAACT GCGGCGCTGT TATCCTAAAC 
ATCACAAGGA AAGGTGGCGA CATCAACAAG ACAATCTACG TGGATTACAA GACTGAGGAC 
GGTTCAGCTA ATGCTGGGGC TGACTATGAG TTCACAGAGG GCACAGTGGT GTTCAAACCA 
GGGGAGATGA TCAAAGAAAT AAGCATTGGC ATCATTGATG ATGACATCTT TGAAGAGGAT 
GAGCACTTTT TTGTGCGTCT CAGTAATCTC CGTGTTCTGG AAACTGAGGA TGAGGTCCTG 
TCTGCCAACA GCCTCCCATA CCCAAAGGCA ATGCTAGGAT TCCCATCTGT GGCCACAGTG 
ACTATTCTGG ATGATGATCA CTCTGGCATC TTCACTTTTG AGAGTAGTCC AGTCCATGTT 
AGTGAGAGCA TTGGCATCAT GGAGGTAAAG GTGTTGAGGA CCTCTGGAGC AAGGGGAACA 
GTCATTGTGC CTTATCGCAC CGTGGAGGGA CTTGCCAAAG GAGGAGGAGA GGACTTTGAA 
GACACCTACG GAGAACTGGA GTTCAAAAAC GATGAGACCT GCAAATTTAT TCACGTGAAA 
GTTATTGACG ATGAGGAGTA TGAGAAAAAC AAGAACTTCT TCCTGGAGCT GGCTGAGCCT 
CGCATGGTAG ACATGAGTCT GCAGAAAGCC ATGTTGTTAG CTGATGATGT TCCAGACAGG 
AAACTTTCCT CAGATGAGGC GGAAGCCAAG CGGATTGCAG AAATGGGGAA ACCAGTCCTG 
GGGGAGCACC CCAGACTGGA GGTCATCATT GAAGAATCAT ATGAGTTTAA GAGCACAGTG 
GACAAGCTGA TCAAGAAGAC CAACCTGGCT CTGGTGGTGG GAACAAACTC CTGGAGAGAG 
CAGTTCATGG AGGCTATCAC AGTCAGTGCA GATGAGGACG AGGATGACAC GGGAGAAGAA 
CGGCTTCCTT CCTGCTTTGA CTACGTCATG CACTTCCTCA CCGTCTTCTG GAAGGTTCTG 
TTTGCCTGCG TTCCTCCCAC CGACTACTTG AATGGTTGGG CCTGCTTCGC TGTCTCCATC 
ATCATCATCG GTCTGCTCAC AGCCGTCATC GGCGACCTGG CGTCCCACTT TGGCTGCACC 
ATCGGCCTGA AGGACTCCGT CACCGCTGTG GTTTTTGTCG CGCTTGGCAC TTCAGTCCCA 
GACACCTTTG CCAGCAAGGT AGCAGCAGTC CAGGACACCT ACGCAGATGC CTCCATCGGG 
AACGTGACGG GCAGCAATGC CGTCAACGTC TTCCTGGGAA TCGGCATGGC CTGGTCGGTG 
GCAGCCATCT ACTGGCACAT GAAAGGGAAG TCGTTTGTCG TGCAGGCCGG CTCGCTGGCA 
TTCTCTGTCA CCCTTTTCAC CATCTTCGCC TTCTTGGCCG TCTCGGTGCT GCTCTACCGG 
CGCCGGGCCC ACATCGGAGG AGAACTGGGA GGCTCCCGGA GACACAGACT GGCCACATCA 
GCCTTCTTTT TCAGCCTCTG GTTTCTTTAC ATTCTCTTCT CCAGTCTGGA GGCCTACTGT 
CATATAGAAG GTTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015824655.1
CDS	1026..3803
Translation	MEGSRPVKPGSSACLWLGLISVAVAFLCSEARTTPSPLLSINNATCKGTSKCQAGIILPIWYPEDPSMGDKIARVIVYFVAMIYMFLGVSIIADRFMAAIEVITSQEREIVIKRPNGETTTTTIRVWNETVSNLTLMALGSSAPEILLSLIEVCGHEFKSGELGPSTIVGSAAFNMFVIIGLCVSVIPQGEVRKVKHLRVFFVTAGWSIFAYIWLYMILAVFSPNVVQVWEGLVTLAFFPICVILAWVADRRLLFYKFMHKKYRTDKHRGVIIETETERSKGIEMDGKMVNSHFMDGGAASNLIGLIESKEVDESRRDMIRILKDLKQKYPEKELDQLVEMANYYALSHQQKSRAFYRIQATRMMTGAGNILKKHVAEQAKKSASVQEVHVDEPEEFVSRIAFEPAVYQCLENCGAVILNITRKGGDINKTIYVDYKTEDGSANAGADYEFTEGTVVFKPGEMIKEISIGIIDDDIFEEDEHFFVRLSNLRVLETEDEVLSANSLPYPKAMLGFPSVATVTILDDDHSGIFTFESSPVHVSESIGIMEVKVLRTSGARGTVIVPYRTVEGLAKGGGEDFEDTYGELEFKNDETCKFIHVKVIDDEEYEKNKNFFLELAEPRMVDMSLQKAMLLADDVPDRKLSSDEAEAKRIAEMGKPVLGEHPRLEVIIEESYEFKSTVDKLIKKTNLALVVGTNSWREQFMEAITVSADEDEDDTGEERLPSCFDYVMHFLTVFWKVLFACVPPTDYLNGWACFAVSIIIIGLLTAVIGDLASHFGCTIGLKDSVTAVVFVALGTSVPDTFASKVAAVQDTYADASIGNVTGSNAVNVFLGIGMAWSVAAIYWHMKGKSFVVQAGSLAFSVTLFTIFAFLAVSVLLYRRRAHIGGELGGSRRHRLATSAFFFSLWFLYILFSSLEAYCHIEGF

Target ORF information:

RefSeq Version	XM_015969169.1
Organism	Nothobranchius furzeri(turquoise killifish)
Definition	Nothobranchius furzeri solute carrier family 8 member A3 (slc8a3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015969169.1

ORF Insert Sequence:

ATGGAGGGTT CAAGGCCTGT AAAACCGGGG TCATCAGCCT GCCTCTGGTT GGGGCTGATC 
TCTGTGGCTG TTGCTTTCCT TTGTTCTGAG GCCCGGACCA CTCCCAGCCC TTTGCTAAGC 
ATCAACAATG CCACTTGCAA AGGAACCTCG AAGTGTCAAG CAGGGATCAT CCTTCCCATC 
TGGTATCCAG AGGATCCTTC CATGGGGGAC AAGATTGCTC GAGTCATTGT CTATTTTGTG 
GCCATGATCT ACATGTTTCT TGGTGTATCC ATCATTGCTG ACCGCTTCAT GGCAGCCATT 
GAGGTCATCA CCTCCCAGGA AAGAGAAATT GTTATCAAAA GGCCCAATGG GGAAACAACC 
ACAACCACAA TCCGGGTGTG GAACGAAACA GTCTCCAATC TTACCCTCAT GGCCTTGGGC 
TCCTCTGCTC CTGAGATTTT GCTCTCTCTG ATTGAGGTTT GTGGACATGA GTTTAAATCT 
GGTGAACTTG GCCCTTCCAC TATTGTTGGC AGTGCAGCTT TCAATATGTT TGTCATAATT 
GGCCTTTGTG TGTCTGTCAT TCCCCAAGGA GAAGTACGAA AAGTCAAGCA CCTCAGAGTG 
TTCTTTGTCA CAGCAGGGTG GAGTATCTTT GCCTACATCT GGCTTTACAT GATTCTGGCG 
GTGTTTTCTC CCAACGTAGT CCAGGTCTGG GAAGGTCTAG TCACACTGGC CTTCTTCCCC 
ATATGTGTTA TTCTTGCCTG GGTAGCAGAC AGACGACTGT TATTCTACAA GTTTATGCAC 
AAGAAGTATC GCACTGATAA ACACAGGGGC GTCATTATTG AGACTGAGAC CGAACGCTCC 
AAGGGAATTG AAATGGACGG CAAGATGGTC AACTCCCACT TCATGGATGG AGGTGCAGCC 
AGCAATCTTA TTGGTCTGAT TGAGAGCAAA GAAGTAGACG AATCTCGTCG AGACATGATT 
CGTATCTTAA AGGACCTAAA GCAGAAGTAC CCTGAGAAAG AGCTTGATCA ACTGGTTGAG 
ATGGCCAACT ACTATGCCCT CTCACACCAG CAAAAGAGCC GTGCCTTCTA CCGTATACAG 
GCCACACGCA TGATGACAGG CGCTGGAAAT ATCCTGAAGA AACACGTTGC AGAGCAGGCA 
AAGAAGAGCG CTAGTGTGCA GGAGGTGCAC GTGGATGAAC CAGAAGAGTT TGTGTCTCGG 
ATAGCTTTTG AGCCCGCTGT CTATCAGTGC CTTGAGAACT GCGGCGCTGT TATCCTAAAC 
ATCACAAGGA AAGGTGGCGA CATCAACAAG ACAATCTACG TGGATTACAA GACTGAGGAC 
GGTTCAGCTA ATGCTGGGGC TGACTATGAG TTCACAGAGG GCACAGTGGT GTTCAAACCA 
GGGGAGATGA TCAAAGAAAT AAGCATTGGC ATCATTGATG ATGACATCTT TGAAGAGGAT 
GAGCACTTTT TTGTGCGTCT CAGTAATCTC CGTGTTCTGG AAACTGAGGA TGAGGTCCTG 
TCTGCCAACA GCCTCCCATA CCCAAAGGCA ATGCTAGGAT TCCCATCTGT GGCCACAGTG 
ACTATTCTGG ATGATGATCA CTCTGGCATC TTCACTTTTG AGAGTAGTCC AGTCCATGTT 
AGTGAGAGCA TTGGCATCAT GGAGGTAAAG GTGTTGAGGA CCTCTGGAGC AAGGGGAACA 
GTCATTGTGC CTTATCGCAC CGTGGAGGGA CTTGCCAAAG GAGGAGGAGA GGACTTTGAA 
GACACCTACG GAGAACTGGA GTTCAAAAAC GATGAGACCT GCAAATTTAT TCACGTGAAA 
GTTATTGACG ATGAGGAGTA TGAGAAAAAC AAGAACTTCT TCCTGGAGCT GGCTGAGCCT 
CGCATGGTAG ACATGAGTCT GCAGAAAGCC ATGTTGTTAG CTGATGATGT TCCAGACAGG 
AAACTTTCCT CAGATGAGGC GGAAGCCAAG CGGATTGCAG AAATGGGGAA ACCAGTCCTG 
GGGGAGCACC CCAGACTGGA GGTCATCATT GAAGAATCAT ATGAGTTTAA GAGCACAGTG 
GACAAGCTGA TCAAGAAGAC CAACCTGGCT CTGGTGGTGG GAACAAACTC CTGGAGAGAG 
CAGTTCATGG AGGCTATCAC AGTCAGTGCA GATGAGGACG AGGATGACAC GGGAGAAGAA 
CGGCTTCCTT CCTGCTTTGA CTACGTCATG CACTTCCTCA CCGTCTTCTG GAAGGTTCTG 
TTTGCCTGCG TTCCTCCCAC CGACTACTTG AATGGTTGGG CCTGCTTCGC TGTCTCCATC 
ATCATCATCG GTCTGCTCAC AGCCGTCATC GGCGACCTGG CGTCCCACTT TGGCTGCACC 
ATCGGCCTGA AGGACTCCGT CACCGCTGTG GTTTTTGTCG CGCTTGGCAC TTCAGTCCCA 
GACACCTTTG CCAGCAAGGT AGCAGCAGTC CAGGACACCT ACGCAGATGC CTCCATCGGG 
AACGTGACGG GCAGCAATGC CGTCAACGTC TTCCTGGGAA TCGGCATGGC CTGGTCGGTG 
GCAGCCATCT ACTGGCACAT GAAAGGGAAG TCGTTTGTCG TGCAGGCCGG CTCGCTGGCA 
TTCTCTGTCA CCCTTTTCAC CATCTTCGCC TTCTTGGCCG TCTCGGTGCT GCTCTACCGG 
CGCCGGGCCC ACATCGGAGG AGAACTGGGA GGCTCCCGGA GACACAGACT GGCCACATCA 
GCCTTCTTTT TCAGCCTCTG GTTTCTTTAC ATTCTCTTCT CCAGTCTGGA GGCCTACTGT 
CATATAGAAG GTTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

slc8a3 ( XM_015969170.1 ) cDNA ORF clone, Nothobranchius furzeri(turquoise killifish) -> XP_015824656.1 Nothobranchius furzeri solute carrier family 8 member A3 (slc8a3), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ONo24619
Clone ID Related Accession (Same CDS sequence)	XM_015969170.1
Accession Version	XM_015969170.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2760bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-03-17
Organism	Nothobranchius furzeri(turquoise killifish)
Product	sodium/calcium exchanger 3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015443591.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Nothobranchius furzeri Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGGTT CAAGGCCTGT AAAACCGGGG TCATCAGCCT GCCTCTGGTT GGGGCTGATC 
TCTGTGGCTG TTGCTTTCCT TTGTTCTGAG GCCCGGACCA CTCCCAGCCC TTTGCTAAGC 
ATCAACAATG CCACTTGCAA AGGAACCTCG AAGTGTCAAG CAGGGATCAT CCTTCCCATC 
TGGTATCCAG AGGATCCTTC CATGGGGGAC AAGATTGCTC GAGTCATTGT CTATTTTGTG 
GCCATGATCT ACATGTTTCT TGGTGTATCC ATCATTGCTG ACCGCTTCAT GGCAGCCATT 
GAGGTCATCA CCTCCCAGGA AAGAGAAATT GTTATCAAAA GGCCCAATGG GGAAACAACC 
ACAACCACAA TCCGGGTGTG GAACGAAACA GTCTCCAATC TTACCCTCAT GGCCTTGGGC 
TCCTCTGCTC CTGAGATTTT GCTCTCTCTG ATTGAGGTTT GTGGACATGA GTTTAAATCT 
GGTGAACTTG GCCCTTCCAC TATTGTTGGC AGTGCAGCTT TCAATATGTT TGTCATAATT 
GGCCTTTGTG TGTCTGTCAT TCCCCAAGGA GAAGTACGAA AAGTCAAGCA CCTCAGAGTG 
TTCTTTGTCA CAGCAGGGTG GAGTATCTTT GCCTACATCT GGCTTTACAT GATTCTGGCG 
GTGTTTTCTC CCAACGTAGT CCAGGTCTGG GAAGGTCTAG TCACACTGGC CTTCTTCCCC 
ATATGTGTTA TTCTTGCCTG GGTAGCAGAC AGACGACTGT TATTCTACAA GTTTATGCAC 
AAGAAGTATC GCACTGATAA ACACAGGGGC GTCATTATTG AGACTGAGAC CGAACGCTCC 
AAGGGAATTG AAATGGACGG CAAGATGGTC AACTCCCACT TCATGGATGG AGGTGCAGCC 
AGCAATCTTA TTGGTCTGAT TGAGAGCAAA GAAGTAGACG AATCTCGTCG AGACATGATT 
CGTATCTTAA AGGACCTAAA GCAGAAGTAC CCTGAGAAAG AGCTTGATCA ACTGGTTGAG 
ATGGCCAACT ACTATGCCCT CTCACACCAG CAAAAGAGCC GTGCCTTCTA CCGTATACAG 
GCCACACGCA TGATGACAGG CGCTGGAAAT ATCCTGAAGA AACACGTTGC AGAGCAGGCA 
AAGAAGAGCG CTAGTGTGCA GGAGGTGCAC GTGGATGAAC CAGAAGAGTT TGTGTCTCGG 
ATAGCTTTTG AGCCCGCTGT CTATCAGTGC CTTGAGAACT GCGGCGCTGT TATCCTAAAC 
ATCACAAGGA AAGGTGGCGA CATCAACAAG ACAATCTACG TGGATTACAA GACTGAGGAC 
GGTTCAGCTA ATGCTGGGGC TGACTATGAG TTCACAGAGG GCACAGTGGT GTTCAAACCA 
GGGGAGATGA TCAAAGAAAT AAGCATTGGC ATCATTGATG ATGACATCTT TGAAGAGGAT 
GAGCACTTTT TTGTGCGTCT CAGTAATCTC CGTGTTCTGG AAACTGAGGA TGAGGTCCTG 
TCTGCCAACA GCCTCCCATA CCCAAAGGCA ATGCTAGGAT TCCCATCTGT GGCCACAGTG 
ACTATTCTGG ATGATGATCA CTCTGGCATC TTCACTTTTG AGAGTAGTCC AGTCCATGTT 
AGTGAGAGCA TTGGCATCAT GGAGGTAAAG GTGTTGAGGA CCTCTGGAGC AAGGGGAACA 
GTCATTGTGC CTTATCGCAC CGTGGAGGGA CTTGCCAAAG GAGGAGGAGA GGACTTTGAA 
GACACCTACG GAGAACTGGA GTTCAAAAAC GATGAGACCT GCAAATTTAT TCACGTGAAA 
GTTATTGACG ATGAGGAGTA TGAGAAAAAC AAGAACTTCT TCCTGGAGCT GGCTGAGCCT 
CGCATGGTAG ACATGAGTCT GCAGAAAGAT GTTCCAGACA GGAAACTTTC CTCAGATGAG 
GCGGAAGCCA AGCGGATTGC AGAAATGGGG AAACCAGTCC TGGGGGAGCA CCCCAGACTG 
GAGGTCATCA TTGAAGAATC ATATGAGTTT AAGAGCACAG TGGACAAGCT GATCAAGAAG 
ACCAACCTGG CTCTGGTGGT GGGAACAAAC TCCTGGAGAG AGCAGTTCAT GGAGGCTATC 
ACAGTCAGTG CAGATGAGGA CGAGGATGAC ACGGGAGAAG AACGGCTTCC TTCCTGCTTT 
GACTACGTCA TGCACTTCCT CACCGTCTTC TGGAAGGTTC TGTTTGCCTG CGTTCCTCCC 
ACCGACTACT TGAATGGTTG GGCCTGCTTC GCTGTCTCCA TCATCATCAT CGGTCTGCTC 
ACAGCCGTCA TCGGCGACCT GGCGTCCCAC TTTGGCTGCA CCATCGGCCT GAAGGACTCC 
GTCACCGCTG TGGTTTTTGT CGCGCTTGGC ACTTCAGTCC CAGACACCTT TGCCAGCAAG 
GTAGCAGCAG TCCAGGACAC CTACGCAGAT GCCTCCATCG GGAACGTGAC GGGCAGCAAT 
GCCGTCAACG TCTTCCTGGG AATCGGCATG GCCTGGTCGG TGGCAGCCAT CTACTGGCAC 
ATGAAAGGGA AGTCGTTTGT CGTGCAGGCC GGCTCGCTGG CATTCTCTGT CACCCTTTTC 
ACCATCTTCG CCTTCTTGGC CGTCTCGGTG CTGCTCTACC GGCGCCGGGC CCACATCGGA 
GGAGAACTGG GAGGCTCCCG GAGACACAGA CTGGCCACAT CAGCCTTCTT TTTCAGCCTC 
TGGTTTCTTT ACATTCTCTT CTCCAGTCTG GAGGCCTACT GTCATATAGA AGGTTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015824656.1
CDS	1026..3785
Translation	MEGSRPVKPGSSACLWLGLISVAVAFLCSEARTTPSPLLSINNATCKGTSKCQAGIILPIWYPEDPSMGDKIARVIVYFVAMIYMFLGVSIIADRFMAAIEVITSQEREIVIKRPNGETTTTTIRVWNETVSNLTLMALGSSAPEILLSLIEVCGHEFKSGELGPSTIVGSAAFNMFVIIGLCVSVIPQGEVRKVKHLRVFFVTAGWSIFAYIWLYMILAVFSPNVVQVWEGLVTLAFFPICVILAWVADRRLLFYKFMHKKYRTDKHRGVIIETETERSKGIEMDGKMVNSHFMDGGAASNLIGLIESKEVDESRRDMIRILKDLKQKYPEKELDQLVEMANYYALSHQQKSRAFYRIQATRMMTGAGNILKKHVAEQAKKSASVQEVHVDEPEEFVSRIAFEPAVYQCLENCGAVILNITRKGGDINKTIYVDYKTEDGSANAGADYEFTEGTVVFKPGEMIKEISIGIIDDDIFEEDEHFFVRLSNLRVLETEDEVLSANSLPYPKAMLGFPSVATVTILDDDHSGIFTFESSPVHVSESIGIMEVKVLRTSGARGTVIVPYRTVEGLAKGGGEDFEDTYGELEFKNDETCKFIHVKVIDDEEYEKNKNFFLELAEPRMVDMSLQKDVPDRKLSSDEAEAKRIAEMGKPVLGEHPRLEVIIEESYEFKSTVDKLIKKTNLALVVGTNSWREQFMEAITVSADEDEDDTGEERLPSCFDYVMHFLTVFWKVLFACVPPTDYLNGWACFAVSIIIIGLLTAVIGDLASHFGCTIGLKDSVTAVVFVALGTSVPDTFASKVAAVQDTYADASIGNVTGSNAVNVFLGIGMAWSVAAIYWHMKGKSFVVQAGSLAFSVTLFTIFAFLAVSVLLYRRRAHIGGELGGSRRHRLATSAFFFSLWFLYILFSSLEAYCHIEGF

Target ORF information:

RefSeq Version	XM_015969170.1
Organism	Nothobranchius furzeri(turquoise killifish)
Definition	Nothobranchius furzeri solute carrier family 8 member A3 (slc8a3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015969170.1

ORF Insert Sequence:

ATGGAGGGTT CAAGGCCTGT AAAACCGGGG TCATCAGCCT GCCTCTGGTT GGGGCTGATC 
TCTGTGGCTG TTGCTTTCCT TTGTTCTGAG GCCCGGACCA CTCCCAGCCC TTTGCTAAGC 
ATCAACAATG CCACTTGCAA AGGAACCTCG AAGTGTCAAG CAGGGATCAT CCTTCCCATC 
TGGTATCCAG AGGATCCTTC CATGGGGGAC AAGATTGCTC GAGTCATTGT CTATTTTGTG 
GCCATGATCT ACATGTTTCT TGGTGTATCC ATCATTGCTG ACCGCTTCAT GGCAGCCATT 
GAGGTCATCA CCTCCCAGGA AAGAGAAATT GTTATCAAAA GGCCCAATGG GGAAACAACC 
ACAACCACAA TCCGGGTGTG GAACGAAACA GTCTCCAATC TTACCCTCAT GGCCTTGGGC 
TCCTCTGCTC CTGAGATTTT GCTCTCTCTG ATTGAGGTTT GTGGACATGA GTTTAAATCT 
GGTGAACTTG GCCCTTCCAC TATTGTTGGC AGTGCAGCTT TCAATATGTT TGTCATAATT 
GGCCTTTGTG TGTCTGTCAT TCCCCAAGGA GAAGTACGAA AAGTCAAGCA CCTCAGAGTG 
TTCTTTGTCA CAGCAGGGTG GAGTATCTTT GCCTACATCT GGCTTTACAT GATTCTGGCG 
GTGTTTTCTC CCAACGTAGT CCAGGTCTGG GAAGGTCTAG TCACACTGGC CTTCTTCCCC 
ATATGTGTTA TTCTTGCCTG GGTAGCAGAC AGACGACTGT TATTCTACAA GTTTATGCAC 
AAGAAGTATC GCACTGATAA ACACAGGGGC GTCATTATTG AGACTGAGAC CGAACGCTCC 
AAGGGAATTG AAATGGACGG CAAGATGGTC AACTCCCACT TCATGGATGG AGGTGCAGCC 
AGCAATCTTA TTGGTCTGAT TGAGAGCAAA GAAGTAGACG AATCTCGTCG AGACATGATT 
CGTATCTTAA AGGACCTAAA GCAGAAGTAC CCTGAGAAAG AGCTTGATCA ACTGGTTGAG 
ATGGCCAACT ACTATGCCCT CTCACACCAG CAAAAGAGCC GTGCCTTCTA CCGTATACAG 
GCCACACGCA TGATGACAGG CGCTGGAAAT ATCCTGAAGA AACACGTTGC AGAGCAGGCA 
AAGAAGAGCG CTAGTGTGCA GGAGGTGCAC GTGGATGAAC CAGAAGAGTT TGTGTCTCGG 
ATAGCTTTTG AGCCCGCTGT CTATCAGTGC CTTGAGAACT GCGGCGCTGT TATCCTAAAC 
ATCACAAGGA AAGGTGGCGA CATCAACAAG ACAATCTACG TGGATTACAA GACTGAGGAC 
GGTTCAGCTA ATGCTGGGGC TGACTATGAG TTCACAGAGG GCACAGTGGT GTTCAAACCA 
GGGGAGATGA TCAAAGAAAT AAGCATTGGC ATCATTGATG ATGACATCTT TGAAGAGGAT 
GAGCACTTTT TTGTGCGTCT CAGTAATCTC CGTGTTCTGG AAACTGAGGA TGAGGTCCTG 
TCTGCCAACA GCCTCCCATA CCCAAAGGCA ATGCTAGGAT TCCCATCTGT GGCCACAGTG 
ACTATTCTGG ATGATGATCA CTCTGGCATC TTCACTTTTG AGAGTAGTCC AGTCCATGTT 
AGTGAGAGCA TTGGCATCAT GGAGGTAAAG GTGTTGAGGA CCTCTGGAGC AAGGGGAACA 
GTCATTGTGC CTTATCGCAC CGTGGAGGGA CTTGCCAAAG GAGGAGGAGA GGACTTTGAA 
GACACCTACG GAGAACTGGA GTTCAAAAAC GATGAGACCT GCAAATTTAT TCACGTGAAA 
GTTATTGACG ATGAGGAGTA TGAGAAAAAC AAGAACTTCT TCCTGGAGCT GGCTGAGCCT 
CGCATGGTAG ACATGAGTCT GCAGAAAGAT GTTCCAGACA GGAAACTTTC CTCAGATGAG 
GCGGAAGCCA AGCGGATTGC AGAAATGGGG AAACCAGTCC TGGGGGAGCA CCCCAGACTG 
GAGGTCATCA TTGAAGAATC ATATGAGTTT AAGAGCACAG TGGACAAGCT GATCAAGAAG 
ACCAACCTGG CTCTGGTGGT GGGAACAAAC TCCTGGAGAG AGCAGTTCAT GGAGGCTATC 
ACAGTCAGTG CAGATGAGGA CGAGGATGAC ACGGGAGAAG AACGGCTTCC TTCCTGCTTT 
GACTACGTCA TGCACTTCCT CACCGTCTTC TGGAAGGTTC TGTTTGCCTG CGTTCCTCCC 
ACCGACTACT TGAATGGTTG GGCCTGCTTC GCTGTCTCCA TCATCATCAT CGGTCTGCTC 
ACAGCCGTCA TCGGCGACCT GGCGTCCCAC TTTGGCTGCA CCATCGGCCT GAAGGACTCC 
GTCACCGCTG TGGTTTTTGT CGCGCTTGGC ACTTCAGTCC CAGACACCTT TGCCAGCAAG 
GTAGCAGCAG TCCAGGACAC CTACGCAGAT GCCTCCATCG GGAACGTGAC GGGCAGCAAT 
GCCGTCAACG TCTTCCTGGG AATCGGCATG GCCTGGTCGG TGGCAGCCAT CTACTGGCAC 
ATGAAAGGGA AGTCGTTTGT CGTGCAGGCC GGCTCGCTGG CATTCTCTGT CACCCTTTTC 
ACCATCTTCG CCTTCTTGGC CGTCTCGGTG CTGCTCTACC GGCGCCGGGC CCACATCGGA 
GGAGAACTGG GAGGCTCCCG GAGACACAGA CTGGCCACAT CAGCCTTCTT TTTCAGCCTC 
TGGTTTCTTT ACATTCTCTT CTCCAGTCTG GAGGCCTACT GTCATATAGA AGGTTTCTAA