ddx23 cDNA ORF clone, Nothobranchius furzeri(turquoise killifish)

Gene
Clones
gRNAs

Summary

Gene Symbol	ddx23
Entrez Gene ID	107391216
Full Name	DEAD-box helicase 23
Gene Type	protein-coding
Organism	Nothobranchius furzeri(turquoise killifish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015968383.1	XP_015823869.1	probable ATP-dependent RNA helicase DDX23

Pathways from BioSystems
Gene Ontology

The following ddx23 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ddx23 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ONo23999	XM_015968383.1 Latest version!	Nothobranchius furzeri DEAD-box helicase 23 (ddx23), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ddx23 ( XM_015968383.1 ) cDNA ORF clone, Nothobranchius furzeri(turquoise killifish) -> XP_015823869.1 Nothobranchius furzeri DEAD-box helicase 23 (ddx23), mRNA.

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CloneID	ONo23999
Clone ID Related Accession (Same CDS sequence)	XM_015968383.1
Accession Version	XM_015968383.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2409bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-03-17
Organism	Nothobranchius furzeri(turquoise killifish)
Product	probable ATP-dependent RNA helicase DDX23
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015443587.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Nothobranchius furzeri Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGCAG AGTCCGCAGA CAGGAAGGAT GCAGAATCCT CAGGAACCAA AGACAGAGAG 
AGGAAACGCA GTCGTTCACG AGATCGAGAC CGTAAAGGGT CACCTTTACG CAAACGTCAT 
CGTTCTCGTG AACGAAACCG CTCCAAGTCT CCAGACAGAG ACCGCCGCCT GAAAGACAGA 
GACAAGGATC GTGACAGAGA CAAAGACAGG GAGAGGAGCC GTAAGGACAG AGACAGCCAT 
CGACGGGATA AAGATCACAA GAGATCCAGA AGCCCTTCAC CAAGGTCTAA GGATGGCAAG 
ATGAAGAAAG AGAAGGATAT AAAAGCAGAG GAAGAAGAAG ATGAAAAAAA GAAAGAAAAG 
GTCCAGCCAC TGTCTCTGGA GGAGCTTCTA GCCAAGAAAA AAGCAGAGGA GGAAGCTGAA 
GCAAAGCCCA AATTCTTGTC CAGAGCAGAG CGAGAAGCTG AGGCGTTGAA ACGCAGAGAG 
CAGCAGGTGG AGGAGAGGAA GAAGATGCTG GAAGATGAGA GAAAGAAAAG GAGGGTGTTT 
CAGGATATTG GGAGAAAAAT GCTGGAGGAC CCTCAAGAAA GGGAGAGACG AGAGCGAAGA 
GAACGAATGG AGCGCGAGAA CAATGGGAAT GAAGAGGATG ATGGGCGGCA GAAGCTCAGA 
GAGGAGAAAG ATAAAAGCAA GGAGCTCGTA GCCATCAAGG AGCGTTATTT GGGTGGCATC 
AAAAAACGTC GGAGAACTCG TCACTTGAAC GACAGGAAGT TTGTGTTTGA ATGGGATGCC 
TCCGAGGACA CTTCGGTTGA CTACAACCCC ATCTATAAAG AGAAACACCA GGTGCAGCTG 
TACGGACGTG GCTTCATCGC TGGTATCGAC CTAAAGCAGC AGAAAAGAGA GCAGTCCCGT 
TTCTATGGTG ATCTGATGGA GAAGAGACGC ACACTCGAGG AGAAGGAGCA GGAGGAGACG 
AGACTGAAGA AGGTGCGTAA GAAGGAAGCC AAGCAGCGCT GGGACGACAG ACACTGGTCT 
CAGAAGAAGA TGGACGAGAT GACGGACAGA GACTGGCGTA TCTTCAGAGA GGATTATAGC 
ATCACCACCA AAGGAGGAAA GATCCCGAAC CCCATCAGGA ACTGGAAGGA GTACGCGCTG 
CCCGCCCACA TCCTGGAGGT CATCGACAAA TGTGGCTATA AGGATCCAAC TCCAATTCAG 
AGGCAAGCCA TTCCTATTGG CTTGCAGAAC CGGGACATTA TTGGTGTGGC AGAGACGGGT 
AGTGGGAAAA CGGCAGCCTT CCTGATTCCT CTGCTGGTCT GGATTACCAC TCTCCCCAAG 
ATTGACAGGA TCGAAGACTC GGATCAGGGT CCTTATGCTG TGATTTTGGC TCCAACTCGT 
GAGCTGGCTC AGCAGATCGA AGAGGAAACC ATAAAGTTTG GCAAACCCCT TGGTATCCGA 
ACAGTAGCCG TGATCGGAGG AATCTCCAGG GAGGACCAAG GCTTCCGCCT CAGGATGGGC 
TGTGAGATTG TAATCGCCAC TCCCGGTCGT CTGATCGATG TGCTGGAGAA CCGGTACTTG 
GTTCTGGGCC GCTGCACCTA CGTAGTCCTG GATGAGGCCG ACCGTATGAT CGACATGGGC 
TTCGAGCCTG ACGTCCAGAA GATCCTGGAG TACATCCCGG TGACCAATCA AAAACCAGAC 
ACAGATGAAG CAGAGGACCC TGAGAAGATG ATGATGAACT TTGAGTCTGG GAAACACAAA 
TACAGACAGA CGGTCATGTT CACCGCTACG ATGCCTCCAG CTGTGGAGCG ACTGGCCCGG 
AGCTACCTGA GACGTCCTGC TGTGGTGTAC ATCGGATCAG CTGGAAAACC TCACGAGCGG 
GTCGAACAGA AGGTGCTGCT GATGTCAGAA GGAGAGAAGA GGAAGAAGTT GCTGGAAGTT 
TTGTCGCGCG GCTTCGAGCC TCCCATCATC ATCTTCGTCA ACCAGAAGAA GGGCTGCGAT 
GTGCTGGCTA AATCTCTGGA GAAGATGGGG TACAATGCGT GCACGCTGCA TGGTGGCAAA 
GGCCAGGAGC AGAGAGAGTT TGCCCTCTCC AATCTCAAGG CAGGAGCCAA AGATATCCTG 
GTGGCCACAG ATGTTGCTGG TCGAGGTATC GACATCCAGG ACGTCTCCAT GGTCATCAAC 
TATGACATGG CAAAGAACAT TGAAGATTAC ATCCATCGTA TTGGTCGTAC TGGTCGTGCC 
GGCAAGAGCG GTGTGGCCAT GACTTTCCTC ACCAAGGAGG ACTCTGCTGT CTTTTATGAC 
TTGAAGCAGG CAATCCTGGA GAGCCCGGTG TCCACCTGTC CTCCGGAGCT GACCAACCAC 
CCCGATGCGC AGCACAAACC TGGAACCATC CTTACCAAGA AGAGACGCGA GGAGACCATA 
TTTGCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015823869.1
CDS	120..2528
Translation	MAAESADRKDAESSGTKDRERKRSRSRDRDRKGSPLRKRHRSRERNRSKSPDRDRRLKDRDKDRDRDKDRERSRKDRDSHRRDKDHKRSRSPSPRSKDGKMKKEKDIKAEEEEDEKKKEKVQPLSLEELLAKKKAEEEAEAKPKFLSRAEREAEALKRREQQVEERKKMLEDERKKRRVFQDIGRKMLEDPQERERRERRERMERENNGNEEDDGRQKLREEKDKSKELVAIKERYLGGIKKRRRTRHLNDRKFVFEWDASEDTSVDYNPIYKEKHQVQLYGRGFIAGIDLKQQKREQSRFYGDLMEKRRTLEEKEQEETRLKKVRKKEAKQRWDDRHWSQKKMDEMTDRDWRIFREDYSITTKGGKIPNPIRNWKEYALPAHILEVIDKCGYKDPTPIQRQAIPIGLQNRDIIGVAETGSGKTAAFLIPLLVWITTLPKIDRIEDSDQGPYAVILAPTRELAQQIEEETIKFGKPLGIRTVAVIGGISREDQGFRLRMGCEIVIATPGRLIDVLENRYLVLGRCTYVVLDEADRMIDMGFEPDVQKILEYIPVTNQKPDTDEAEDPEKMMMNFESGKHKYRQTVMFTATMPPAVERLARSYLRRPAVVYIGSAGKPHERVEQKVLLMSEGEKRKKLLEVLSRGFEPPIIIFVNQKKGCDVLAKSLEKMGYNACTLHGGKGQEQREFALSNLKAGAKDILVATDVAGRGIDIQDVSMVINYDMAKNIEDYIHRIGRTGRAGKSGVAMTFLTKEDSAVFYDLKQAILESPVSTCPPELTNHPDAQHKPGTILTKKRREETIFA

Target ORF information:

RefSeq Version	XM_015968383.1
Organism	Nothobranchius furzeri(turquoise killifish)
Definition	Nothobranchius furzeri DEAD-box helicase 23 (ddx23), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015968383.1

ORF Insert Sequence:

ATGGCAGCAG AGTCCGCAGA CAGGAAGGAT GCAGAATCCT CAGGAACCAA AGACAGAGAG 
AGGAAACGCA GTCGTTCACG AGATCGAGAC CGTAAAGGGT CACCTTTACG CAAACGTCAT 
CGTTCTCGTG AACGAAACCG CTCCAAGTCT CCAGACAGAG ACCGCCGCCT GAAAGACAGA 
GACAAGGATC GTGACAGAGA CAAAGACAGG GAGAGGAGCC GTAAGGACAG AGACAGCCAT 
CGACGGGATA AAGATCACAA GAGATCCAGA AGCCCTTCAC CAAGGTCTAA GGATGGCAAG 
ATGAAGAAAG AGAAGGATAT AAAAGCAGAG GAAGAAGAAG ATGAAAAAAA GAAAGAAAAG 
GTCCAGCCAC TGTCTCTGGA GGAGCTTCTA GCCAAGAAAA AAGCAGAGGA GGAAGCTGAA 
GCAAAGCCCA AATTCTTGTC CAGAGCAGAG CGAGAAGCTG AGGCGTTGAA ACGCAGAGAG 
CAGCAGGTGG AGGAGAGGAA GAAGATGCTG GAAGATGAGA GAAAGAAAAG GAGGGTGTTT 
CAGGATATTG GGAGAAAAAT GCTGGAGGAC CCTCAAGAAA GGGAGAGACG AGAGCGAAGA 
GAACGAATGG AGCGCGAGAA CAATGGGAAT GAAGAGGATG ATGGGCGGCA GAAGCTCAGA 
GAGGAGAAAG ATAAAAGCAA GGAGCTCGTA GCCATCAAGG AGCGTTATTT GGGTGGCATC 
AAAAAACGTC GGAGAACTCG TCACTTGAAC GACAGGAAGT TTGTGTTTGA ATGGGATGCC 
TCCGAGGACA CTTCGGTTGA CTACAACCCC ATCTATAAAG AGAAACACCA GGTGCAGCTG 
TACGGACGTG GCTTCATCGC TGGTATCGAC CTAAAGCAGC AGAAAAGAGA GCAGTCCCGT 
TTCTATGGTG ATCTGATGGA GAAGAGACGC ACACTCGAGG AGAAGGAGCA GGAGGAGACG 
AGACTGAAGA AGGTGCGTAA GAAGGAAGCC AAGCAGCGCT GGGACGACAG ACACTGGTCT 
CAGAAGAAGA TGGACGAGAT GACGGACAGA GACTGGCGTA TCTTCAGAGA GGATTATAGC 
ATCACCACCA AAGGAGGAAA GATCCCGAAC CCCATCAGGA ACTGGAAGGA GTACGCGCTG 
CCCGCCCACA TCCTGGAGGT CATCGACAAA TGTGGCTATA AGGATCCAAC TCCAATTCAG 
AGGCAAGCCA TTCCTATTGG CTTGCAGAAC CGGGACATTA TTGGTGTGGC AGAGACGGGT 
AGTGGGAAAA CGGCAGCCTT CCTGATTCCT CTGCTGGTCT GGATTACCAC TCTCCCCAAG 
ATTGACAGGA TCGAAGACTC GGATCAGGGT CCTTATGCTG TGATTTTGGC TCCAACTCGT 
GAGCTGGCTC AGCAGATCGA AGAGGAAACC ATAAAGTTTG GCAAACCCCT TGGTATCCGA 
ACAGTAGCCG TGATCGGAGG AATCTCCAGG GAGGACCAAG GCTTCCGCCT CAGGATGGGC 
TGTGAGATTG TAATCGCCAC TCCCGGTCGT CTGATCGATG TGCTGGAGAA CCGGTACTTG 
GTTCTGGGCC GCTGCACCTA CGTAGTCCTG GATGAGGCCG ACCGTATGAT CGACATGGGC 
TTCGAGCCTG ACGTCCAGAA GATCCTGGAG TACATCCCGG TGACCAATCA AAAACCAGAC 
ACAGATGAAG CAGAGGACCC TGAGAAGATG ATGATGAACT TTGAGTCTGG GAAACACAAA 
TACAGACAGA CGGTCATGTT CACCGCTACG ATGCCTCCAG CTGTGGAGCG ACTGGCCCGG 
AGCTACCTGA GACGTCCTGC TGTGGTGTAC ATCGGATCAG CTGGAAAACC TCACGAGCGG 
GTCGAACAGA AGGTGCTGCT GATGTCAGAA GGAGAGAAGA GGAAGAAGTT GCTGGAAGTT 
TTGTCGCGCG GCTTCGAGCC TCCCATCATC ATCTTCGTCA ACCAGAAGAA GGGCTGCGAT 
GTGCTGGCTA AATCTCTGGA GAAGATGGGG TACAATGCGT GCACGCTGCA TGGTGGCAAA 
GGCCAGGAGC AGAGAGAGTT TGCCCTCTCC AATCTCAAGG CAGGAGCCAA AGATATCCTG 
GTGGCCACAG ATGTTGCTGG TCGAGGTATC GACATCCAGG ACGTCTCCAT GGTCATCAAC 
TATGACATGG CAAAGAACAT TGAAGATTAC ATCCATCGTA TTGGTCGTAC TGGTCGTGCC 
GGCAAGAGCG GTGTGGCCAT GACTTTCCTC ACCAAGGAGG ACTCTGCTGT CTTTTATGAC 
TTGAAGCAGG CAATCCTGGA GAGCCCGGTG TCCACCTGTC CTCCGGAGCT GACCAACCAC 
CCCGATGCGC AGCACAAACC TGGAACCATC CTTACCAAGA AGAGACGCGA GGAGACCATA 
TTTGCTTGA