gad1 cDNA ORF clone, Nothobranchius furzeri(turquoise killifish)

Gene
Clones
gRNAs

Summary

Gene Symbol	gad1
Entrez Gene ID	107390031
Full Name	glutamate decarboxylase 1
Gene Type	protein-coding
Organism	Nothobranchius furzeri(turquoise killifish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015966509.1	XP_015821995.1	glutamate decarboxylase 1

Pathways from BioSystems
Gene Ontology

The following gad1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the gad1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ONo22499	XM_015966509.1 Latest version!	Nothobranchius furzeri glutamate decarboxylase 1 (gad1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

gad1 ( XM_015966509.1 ) cDNA ORF clone, Nothobranchius furzeri(turquoise killifish) -> XP_015821995.1 Nothobranchius furzeri glutamate decarboxylase 1 (gad1), mRNA.

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CloneID	ONo22499
Clone ID Related Accession (Same CDS sequence)	XM_015966509.1
Accession Version	XM_015966509.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1782bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-03-17
Organism	Nothobranchius furzeri(turquoise killifish)
Product	glutamate decarboxylase 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015443582.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Nothobranchius furzeri Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTCTG CACCCTCCTC CTCCTCCTCC TTTGGCGGCG AAGCGGATCC AAACGCGACA 
AATTTACGGC CGCCAGGCTC AAGCTATGAT GCGTGGTGTG GAGTTGCTCA TGGATGTACC 
CGAAAATTGG GAATGAAAAT ATGTGGATTC CTGCAGAAGA ACAACAGTTT GGAGGAGAGG 
AGCAGGATCG TGAGCTCCAT CAAGGAGCGC GCGACCAAAG ATCTGCTGTC CTGCGATAAC 
ACCGGAGGGG ACGCGCGGTT TAAGCGCATA GAGACCGACT TCTCCAATTT GTATGCCAGA 
GAGCTTTTGC CTGCCAAAAA TGGAGAAGAG CCCACCATGC AGTTCCTGCT GGAGGTGGTG 
GAAATCCTCA CCAGCTACAT CAAGAAGACT TTTGACAAAT CCACCAAGGT CCTTGACTTC 
CACTATCCTC ACCAGCTGCT GGAGGGCATA GAGGGCTTCA GTCTGGAGCT GCCCGACCAG 
CCCGAGTCTC TGGAGCAGAT CCTGGTGGAC TGCAGGGACA CCCTGAAATA TGGCGTGAGA 
ACAGGCCACC CCAGGTTCTT TAACCAGCTG TCTACTGGAA TAGACATCGT GGGATTGGCG 
GGAGAGTGGC TCACTTCCAC AGCCAACACA AACATGTTTA CATATGAGAT TGCCCCAGTT 
TTTGTGCTGA TGGAGCAGCT GACGCTCAAA AAGATGAGGG AGATCGTTGG CTGGCCAAAC 
GGAGAAGGCG ATGGAATATT CTCTCCAGGA GGAGCCATTT CCAACATGTA CAGTGTGATG 
ATCGCCAGAT ACAAGTACTT CCCCGAAATC AAGACCAAAG GCATGGCGGC TGCACCTCGG 
CTAGTGCTCT TCACGTCGGA GCTGAGCCAC TACTCCATCA AGAAGGCGAG TGCAGCGCTG 
GGATTTGGGA CAGACAACCT GATCCTTCTA AACACAGACG AGAGAGGAAG AGTTATTCCT 
GCTGATTTGG AGTCCAAAGT AACTGAGGCC AAACAAAAGG GATACGTTCC GGTGTATGTG 
AACGCCACCG CAGGGACCAC CGTGTTCGGA GCCTTTGACC CCATCAACGA CATCGCAGAC 
ATCTGTGAAA AGTACAACAT GTGGCTTCAT GTGGACGCTG CATGGGGAGG AGGTCTGCTG 
ATGTCCAGCA AACACAGACA CAAGATGAGC GGAATCCACA GGGCTAACTC AGTCACCTGG 
AACCCTCACA AGATGATGGG AGTGCCTCTG CAGTGTTCTG CCATTCTGGT CCGAGAGAGG 
GGGCTGTTGC AAGGCTGCAA CTCCATGTGT GCAGGTTACC TCTTCCAGCC AGACAAACAT 
TACGATGTCA CGTACGACAC AGGAGACAAG GCCATCCAGT GTGGACGACA CGTCGACATC 
TTTAAGTTTT GGCTCATGTG GAAGGCAAAG GGAACGGTGG GATTTGAGCA GCAAATTGAC 
AAGTGCCTGG ACCTGTCAGC ATACCTCTAC AGTAAAATTA AAAACAGAGA AGGCTTCCAG 
ATGGTGTTCA ATGGAGAGCC ACAGCACACT AATGTCTGCT TTTGGTACCT TCCGCTGAGC 
CTGCGCCACT TGCCCGATGG TGAGGAAAAA CGCGAGAGAC TGCACAAGGT GGCTCCAAAG 
ATCAAGGCGA TGATGATGGA GTCGGGCACC ACGATGGTGG GTTACCAGCC ACAAGGAGAC 
CAGGTCAACT TCTTCCGCAT GGTGATCTCC AACCCTGCTG CCACCAGGTC AGACATTGAC 
TTCCTGGTTG AGGAGATCGA GCGGCTGGGC CAGGACTTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015821995.1
CDS	609..2390
Translation	MASAPSSSSSFGGEADPNATNLRPPGSSYDAWCGVAHGCTRKLGMKICGFLQKNNSLEERSRIVSSIKERATKDLLSCDNTGGDARFKRIETDFSNLYARELLPAKNGEEPTMQFLLEVVEILTSYIKKTFDKSTKVLDFHYPHQLLEGIEGFSLELPDQPESLEQILVDCRDTLKYGVRTGHPRFFNQLSTGIDIVGLAGEWLTSTANTNMFTYEIAPVFVLMEQLTLKKMREIVGWPNGEGDGIFSPGGAISNMYSVMIARYKYFPEIKTKGMAAAPRLVLFTSELSHYSIKKASAALGFGTDNLILLNTDERGRVIPADLESKVTEAKQKGYVPVYVNATAGTTVFGAFDPINDIADICEKYNMWLHVDAAWGGGLLMSSKHRHKMSGIHRANSVTWNPHKMMGVPLQCSAILVRERGLLQGCNSMCAGYLFQPDKHYDVTYDTGDKAIQCGRHVDIFKFWLMWKAKGTVGFEQQIDKCLDLSAYLYSKIKNREGFQMVFNGEPQHTNVCFWYLPLSLRHLPDGEEKRERLHKVAPKIKAMMMESGTTMVGYQPQGDQVNFFRMVISNPAATRSDIDFLVEEIERLGQDL

Target ORF information:

RefSeq Version	XM_015966509.1
Organism	Nothobranchius furzeri(turquoise killifish)
Definition	Nothobranchius furzeri glutamate decarboxylase 1 (gad1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015966509.1

ORF Insert Sequence:

ATGGCGTCTG CACCCTCCTC CTCCTCCTCC TTTGGCGGCG AAGCGGATCC AAACGCGACA 
AATTTACGGC CGCCAGGCTC AAGCTATGAT GCGTGGTGTG GAGTTGCTCA TGGATGTACC 
CGAAAATTGG GAATGAAAAT ATGTGGATTC CTGCAGAAGA ACAACAGTTT GGAGGAGAGG 
AGCAGGATCG TGAGCTCCAT CAAGGAGCGC GCGACCAAAG ATCTGCTGTC CTGCGATAAC 
ACCGGAGGGG ACGCGCGGTT TAAGCGCATA GAGACCGACT TCTCCAATTT GTATGCCAGA 
GAGCTTTTGC CTGCCAAAAA TGGAGAAGAG CCCACCATGC AGTTCCTGCT GGAGGTGGTG 
GAAATCCTCA CCAGCTACAT CAAGAAGACT TTTGACAAAT CCACCAAGGT CCTTGACTTC 
CACTATCCTC ACCAGCTGCT GGAGGGCATA GAGGGCTTCA GTCTGGAGCT GCCCGACCAG 
CCCGAGTCTC TGGAGCAGAT CCTGGTGGAC TGCAGGGACA CCCTGAAATA TGGCGTGAGA 
ACAGGCCACC CCAGGTTCTT TAACCAGCTG TCTACTGGAA TAGACATCGT GGGATTGGCG 
GGAGAGTGGC TCACTTCCAC AGCCAACACA AACATGTTTA CATATGAGAT TGCCCCAGTT 
TTTGTGCTGA TGGAGCAGCT GACGCTCAAA AAGATGAGGG AGATCGTTGG CTGGCCAAAC 
GGAGAAGGCG ATGGAATATT CTCTCCAGGA GGAGCCATTT CCAACATGTA CAGTGTGATG 
ATCGCCAGAT ACAAGTACTT CCCCGAAATC AAGACCAAAG GCATGGCGGC TGCACCTCGG 
CTAGTGCTCT TCACGTCGGA GCTGAGCCAC TACTCCATCA AGAAGGCGAG TGCAGCGCTG 
GGATTTGGGA CAGACAACCT GATCCTTCTA AACACAGACG AGAGAGGAAG AGTTATTCCT 
GCTGATTTGG AGTCCAAAGT AACTGAGGCC AAACAAAAGG GATACGTTCC GGTGTATGTG 
AACGCCACCG CAGGGACCAC CGTGTTCGGA GCCTTTGACC CCATCAACGA CATCGCAGAC 
ATCTGTGAAA AGTACAACAT GTGGCTTCAT GTGGACGCTG CATGGGGAGG AGGTCTGCTG 
ATGTCCAGCA AACACAGACA CAAGATGAGC GGAATCCACA GGGCTAACTC AGTCACCTGG 
AACCCTCACA AGATGATGGG AGTGCCTCTG CAGTGTTCTG CCATTCTGGT CCGAGAGAGG 
GGGCTGTTGC AAGGCTGCAA CTCCATGTGT GCAGGTTACC TCTTCCAGCC AGACAAACAT 
TACGATGTCA CGTACGACAC AGGAGACAAG GCCATCCAGT GTGGACGACA CGTCGACATC 
TTTAAGTTTT GGCTCATGTG GAAGGCAAAG GGAACGGTGG GATTTGAGCA GCAAATTGAC 
AAGTGCCTGG ACCTGTCAGC ATACCTCTAC AGTAAAATTA AAAACAGAGA AGGCTTCCAG 
ATGGTGTTCA ATGGAGAGCC ACAGCACACT AATGTCTGCT TTTGGTACCT TCCGCTGAGC 
CTGCGCCACT TGCCCGATGG TGAGGAAAAA CGCGAGAGAC TGCACAAGGT GGCTCCAAAG 
ATCAAGGCGA TGATGATGGA GTCGGGCACC ACGATGGTGG GTTACCAGCC ACAAGGAGAC 
CAGGTCAACT TCTTCCGCAT GGTGATCTCC AACCCTGCTG CCACCAGGTC AGACATTGAC 
TTCCTGGTTG AGGAGATCGA GCGGCTGGGC CAGGACTTGT AG