aff3 cDNA ORF clone, Nothobranchius furzeri(turquoise killifish)

Gene
Clones
gRNAs

Summary

Gene Symbol	aff3
Entrez Gene ID	107389780
Full Name	AF4/FMR2 family member 3
Gene Type	protein-coding
Organism	Nothobranchius furzeri(turquoise killifish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015966131.1	XP_015821617.1	AF4/FMR2 family member 3

Pathways from BioSystems
Gene Ontology

The following aff3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the aff3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ONo22200	XM_015966131.1 Latest version!	Nothobranchius furzeri AF4/FMR2 family member 3 (aff3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

aff3 ( XM_015966131.1 ) cDNA ORF clone, Nothobranchius furzeri(turquoise killifish) -> XP_015821617.1 Nothobranchius furzeri AF4/FMR2 family member 3 (aff3), mRNA.

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CloneID	ONo22200
Clone ID Related Accession (Same CDS sequence)	XM_015966131.1
Accession Version	XM_015966131.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3558bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-03-17
Organism	Nothobranchius furzeri(turquoise killifish)
Product	AF4/FMR2 family member 3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015443581.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Nothobranchius furzeri Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTCAGT CCTGGCCATC CCAGCAGGCG TTAGTGGGTG ACCAGCAAAT CTTCTACAAC 
TCCAAGGATG CAAAACAATC ATCTGGACAG CAAAGGCAAA ATCGAGGGGA TGCTCCACTC 
CGAATGCCCC GTCACCCTTA TGTGGCACCT CACAAATCGA TGCTGGCTGA TGATCTGAAG 
CTGAGCAGTG ATGACGATGA CACTCAAAGG GCCATTAATG AGTCAACATC GTGGGCGAGA 
CACAGCCTGT CAGCAAAGCG AGCACACACA CATGGCAGGC GGGTGAGACA TTCCAGCTCT 
GACTCCTCAG ACTCTGACTC CAGTGCCGAA TCGGGCAGCA GCAGCGTTCC TTCTCGGAGC 
CCCAGTCCAG AAGTGCACCC AGGTCCCCTG TCGCCTGCCA ACCCGCTGCC ACCGTGCAAC 
GACAAAGAGA CAGATCACCC CTCTCCTACC CAATGGCAGC TAGATAAATG GCTGAAGAGG 
TCAAAGAAAA ACTCTGGCAG CACTGAACGG AATCACTCCT GGAAGGTTCC AGAACGCCAG 
CGATCTCCCC AAATCCAGAG AGCCCCATCG CCGGCTAGAT CATGGGACAG CCATCAGGAG 
TACAGCCCTA GTCAGAGTCC AATACCCAGC CCAAAGTTCA ATCACAGCCC CAACAACAGC 
CCCATTCCCA GTCCTGGTTA CAGCTACTGC CCAAGCCCCA GCCCATTCAC TAGCACCTGT 
CCAAGCCCCA CCCCCAGTCC AAGACACATT TCAGTCCCAA GCCCAGCTCG AAGCTTGTGT 
CCCAGTCCAT GTGGAAGCCC AAGAGCCAGC CGTAGCCCGA GCCCTTTACC AAATCATCCT 
CCAAGAAGCC CAAGTCCCAC TTTACGTACA CCGACCAGAA TTAGTCACTA CCTTGATGGT 
CAGAATCAGA TCCAAGGACA TTCCAGTCTG ACATCTGACC CCCACAGGAC AAGAATTAGA 
CCATGGTTTG CTCCAGTACC CGCCATCAAC ACAAAGACAA CAACCACAAG TGATCATCAT 
CAACCATCGC ATCAACATAG ACCCAAGACT CACCCATCAC AGAACCAAGT CAAAAACCAA 
AGCAGGCTCA CTGCTCCTGT AGTTTTAACC TCCAATCAGA CCCACAACCA CAATCCCACA 
CCTAGACCAA CTTCATATTC CAACGCAAAA CAGCCATCTG ATGCTCACAA AGCTAAAAAC 
ACATCACATG TTGATCAAAT CCAAATTAAC CCTTCCAACC ACAAGTCACG GCCTCCGTTT 
CAGCAGCCAA ATCCAACCAG ACCAAAGCAT CCAGTTTCTT CTGGTCGAGA AGCCAGAGCT 
GCTCACAGTT CTCTTTCACA ACCTACCTAT AAAGCTACAA ATACCTCTGG TGTTGCATCA 
AGCTCTAGGT CCAGTCCTAG CCAAGTGCAT CGGTCAAAAT CGTGGGAGTC CACGGTATCA 
AACTCTGTTC ATGTAAATAA CTCAAAAACT GCAGTTCGGA AGCTTCCAGC CAAGGCACAG 
GAAGTAAACC AGAGAAGATC GAGTTTGAAC CAATCAGAGG GTAGAAAACA GGAGAGAAAG 
GAGGACAGAC ATAATTGTGA GAAACAACGG GGGAAACCAG AGAGAAAAGA AGACAGGAGG 
CTGGCGGAGG AGCAGCTACT GAGACGACCC TGGATCCAAA GCTCAGCGGA AGAGGAAGAG 
GAAGAGGACG TGATAGAGCA ACAGGGTGGG AGAGACGAGG CAGCAAAAGA GGAGAGCAGG 
AGAAAGGAGG AGCAGCTAAA TAAATGGCTA ACAGCTCTGG CCAAAGACAG AGGGCCTGCC 
AACTTTGAGA GACACCGCCT TAGAGAGGGC TCAGACCATC AGGAGAGGAC AACCAAGAGA 
GGGAGATGTG AGGAAGACAG AGGCCCATCG CCCCATCTGT CTTCACACTC TCCTCCAAAC 
CCTCACAGAC TGTCCTCATC ACCACCCTCA TCCTCCACCT CTTCTTCTAA TTCAGACTCA 
GAATCTGAAT TTCTTATTTC AACCGCCAAA GTTTCAGCAG ATTCCATCTC TCACAAGACA 
TTCAGCAAGA AAGGGCAACA AGTCCCCAGC AGACCCAACG CCCCGAGGCC CCAGGCGGTT 
CACTCCAGAG GACCAAACTC GGGCCCTCCT TGTGAGGGGC AGCAAAGTGA GAGGAAGCAG 
AAACTCTACA CCTTGGTTCC ATTTGGACGA AGCGAGAAGG CAGCTCCCAC CTCCCAGCGG 
GGGCTGAGAA ACCTGGTGGT GCACATTGAC CTCTGTCTTC TAAAAAGGGT CCCTGATAGC 
TCAACAAATT CCCCTGTAAA GAAAAACACT TCTTCCTCAT CTTCTTCATC AACCAAGGAC 
AGGCAAAGAG AAGTTATGAA ACACACGCAC GTTCCAGATG CTTCGACAAA AGATGGCAAA 
AGGAAACGCA AGGTGGAGAA CGGAGTGTCA GACCGGGAAT GCAAGAGGAG TCAGCTTTAT 
CCAGATGACC CCCCTGGACC AACAGAATCA TCAGTCACAG CTGAGACTTT AGTGACTGAG 
ACGATGCACA ATGGGTACCT GGAGGAGTAC TTGGACAAGA GGCCAATGTC TCCCTTGTCT 
CCATTGTCTC CATTGTCTCC GCTGCCCCAG AGCCCTGAAC CCACCAAACC TACCTCCAAA 
ACAAAGACAA CTGAGCAGCA GAACTCTCGC ACCCAGAAAA GCAAGGAGAA GAGCAGAGAC 
CAGCCCGTCA AGCCCAAGAT GGAGGTGGAG TGTGTAAAAG TATCAAAACA ACCCCAACCT 
TCGTCCGAGT CCTCCTGGAG ACCTGCTGAC CACAGAGGAG CTGTTCCGCA TGAAACTTCT 
CACCATGCTG AGTACTACTT ACATGAAGCT AAAAGGATGA AGCACCGTGC CGATGCAATG 
GTGGATAAGT TGGGAAAAGC TGTTAACTAT GTTGATGCTG CTCTGTCCTT CATGGAATGT 
GGGAAGGCGA TGGAGGAAGG GCCGCTGGAG GCCAAGTCTC CTTACACCAT GTATTCAGAG 
ACGGTGGAGC TGATCAGGTA CGCCATGAAG CTGAAGAGCC ACTCGGGCCC TGGAGCAAGG 
CAGGAAGACA AACAGCTGGC GGTTCTGTGT TTCCGATGCC TTGCTCTCCT TTACTGGCAG 
ATGTTCCGTT TGAAAAAGGA TCATGCGCTG AAATACTCCA AAGTGCTGCT TGACTATTTC 
AAGAGTTCTC CCAAAGTGCC TACAACACCA CCTGGTTGGA ATGATTGTGG GAAGAGCATG 
GGAGAACACC CGTCTTCACT CTCACCCAAC GCCAGACACC GCAGAAGGGA CCCACCTGGT 
GGCAGCAGCT CATCTCTCAT AAGCATTCCC CAACGCATCC ACCAGATGGC AGCAAACCAC 
CTGAACATCA CCAACAGTGT CCTGTACAGT TATGAGTACT GGGAGGTAGC AGACAACCTT 
GCAAAGGAAA ATAAAGAGTT CTTCAACTAC TTGAATACCT TGTCTGGACC TTTGACTATT 
CACAGCAGCG TTGCTCATGC TGTCCAGTAC ACCAGACAAG CTCTTCAGTG GATACGCATC 
AGTGCCAAAC TTAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015821617.1
CDS	376..3933
Translation	MTQSWPSQQALVGDQQIFYNSKDAKQSSGQQRQNRGDAPLRMPRHPYVAPHKSMLADDLKLSSDDDDTQRAINESTSWARHSLSAKRAHTHGRRVRHSSSDSSDSDSSAESGSSSVPSRSPSPEVHPGPLSPANPLPPCNDKETDHPSPTQWQLDKWLKRSKKNSGSTERNHSWKVPERQRSPQIQRAPSPARSWDSHQEYSPSQSPIPSPKFNHSPNNSPIPSPGYSYCPSPSPFTSTCPSPTPSPRHISVPSPARSLCPSPCGSPRASRSPSPLPNHPPRSPSPTLRTPTRISHYLDGQNQIQGHSSLTSDPHRTRIRPWFAPVPAINTKTTTTSDHHQPSHQHRPKTHPSQNQVKNQSRLTAPVVLTSNQTHNHNPTPRPTSYSNAKQPSDAHKAKNTSHVDQIQINPSNHKSRPPFQQPNPTRPKHPVSSGREARAAHSSLSQPTYKATNTSGVASSSRSSPSQVHRSKSWESTVSNSVHVNNSKTAVRKLPAKAQEVNQRRSSLNQSEGRKQERKEDRHNCEKQRGKPERKEDRRLAEEQLLRRPWIQSSAEEEEEEDVIEQQGGRDEAAKEESRRKEEQLNKWLTALAKDRGPANFERHRLREGSDHQERTTKRGRCEEDRGPSPHLSSHSPPNPHRLSSSPPSSSTSSSNSDSESEFLISTAKVSADSISHKTFSKKGQQVPSRPNAPRPQAVHSRGPNSGPPCEGQQSERKQKLYTLVPFGRSEKAAPTSQRGLRNLVVHIDLCLLKRVPDSSTNSPVKKNTSSSSSSSTKDRQREVMKHTHVPDASTKDGKRKRKVENGVSDRECKRSQLYPDDPPGPTESSVTAETLVTETMHNGYLEEYLDKRPMSPLSPLSPLSPLPQSPEPTKPTSKTKTTEQQNSRTQKSKEKSRDQPVKPKMEVECVKVSKQPQPSSESSWRPADHRGAVPHETSHHAEYYLHEAKRMKHRADAMVDKLGKAVNYVDAALSFMECGKAMEEGPLEAKSPYTMYSETVELIRYAMKLKSHSGPGARQEDKQLAVLCFRCLALLYWQMFRLKKDHALKYSKVLLDYFKSSPKVPTTPPGWNDCGKSMGEHPSSLSPNARHRRRDPPGGSSSSLISIPQRIHQMAANHLNITNSVLYSYEYWEVADNLAKENKEFFNYLNTLSGPLTIHSSVAHAVQYTRQALQWIRISAKLN

Target ORF information:

RefSeq Version	XM_015966131.1
Organism	Nothobranchius furzeri(turquoise killifish)
Definition	Nothobranchius furzeri AF4/FMR2 family member 3 (aff3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015966131.1

ORF Insert Sequence:

ATGACTCAGT CCTGGCCATC CCAGCAGGCG TTAGTGGGTG ACCAGCAAAT CTTCTACAAC 
TCCAAGGATG CAAAACAATC ATCTGGACAG CAAAGGCAAA ATCGAGGGGA TGCTCCACTC 
CGAATGCCCC GTCACCCTTA TGTGGCACCT CACAAATCGA TGCTGGCTGA TGATCTGAAG 
CTGAGCAGTG ATGACGATGA CACTCAAAGG GCCATTAATG AGTCAACATC GTGGGCGAGA 
CACAGCCTGT CAGCAAAGCG AGCACACACA CATGGCAGGC GGGTGAGACA TTCCAGCTCT 
GACTCCTCAG ACTCTGACTC CAGTGCCGAA TCGGGCAGCA GCAGCGTTCC TTCTCGGAGC 
CCCAGTCCAG AAGTGCACCC AGGTCCCCTG TCGCCTGCCA ACCCGCTGCC ACCGTGCAAC 
GACAAAGAGA CAGATCACCC CTCTCCTACC CAATGGCAGC TAGATAAATG GCTGAAGAGG 
TCAAAGAAAA ACTCTGGCAG CACTGAACGG AATCACTCCT GGAAGGTTCC AGAACGCCAG 
CGATCTCCCC AAATCCAGAG AGCCCCATCG CCGGCTAGAT CATGGGACAG CCATCAGGAG 
TACAGCCCTA GTCAGAGTCC AATACCCAGC CCAAAGTTCA ATCACAGCCC CAACAACAGC 
CCCATTCCCA GTCCTGGTTA CAGCTACTGC CCAAGCCCCA GCCCATTCAC TAGCACCTGT 
CCAAGCCCCA CCCCCAGTCC AAGACACATT TCAGTCCCAA GCCCAGCTCG AAGCTTGTGT 
CCCAGTCCAT GTGGAAGCCC AAGAGCCAGC CGTAGCCCGA GCCCTTTACC AAATCATCCT 
CCAAGAAGCC CAAGTCCCAC TTTACGTACA CCGACCAGAA TTAGTCACTA CCTTGATGGT 
CAGAATCAGA TCCAAGGACA TTCCAGTCTG ACATCTGACC CCCACAGGAC AAGAATTAGA 
CCATGGTTTG CTCCAGTACC CGCCATCAAC ACAAAGACAA CAACCACAAG TGATCATCAT 
CAACCATCGC ATCAACATAG ACCCAAGACT CACCCATCAC AGAACCAAGT CAAAAACCAA 
AGCAGGCTCA CTGCTCCTGT AGTTTTAACC TCCAATCAGA CCCACAACCA CAATCCCACA 
CCTAGACCAA CTTCATATTC CAACGCAAAA CAGCCATCTG ATGCTCACAA AGCTAAAAAC 
ACATCACATG TTGATCAAAT CCAAATTAAC CCTTCCAACC ACAAGTCACG GCCTCCGTTT 
CAGCAGCCAA ATCCAACCAG ACCAAAGCAT CCAGTTTCTT CTGGTCGAGA AGCCAGAGCT 
GCTCACAGTT CTCTTTCACA ACCTACCTAT AAAGCTACAA ATACCTCTGG TGTTGCATCA 
AGCTCTAGGT CCAGTCCTAG CCAAGTGCAT CGGTCAAAAT CGTGGGAGTC CACGGTATCA 
AACTCTGTTC ATGTAAATAA CTCAAAAACT GCAGTTCGGA AGCTTCCAGC CAAGGCACAG 
GAAGTAAACC AGAGAAGATC GAGTTTGAAC CAATCAGAGG GTAGAAAACA GGAGAGAAAG 
GAGGACAGAC ATAATTGTGA GAAACAACGG GGGAAACCAG AGAGAAAAGA AGACAGGAGG 
CTGGCGGAGG AGCAGCTACT GAGACGACCC TGGATCCAAA GCTCAGCGGA AGAGGAAGAG 
GAAGAGGACG TGATAGAGCA ACAGGGTGGG AGAGACGAGG CAGCAAAAGA GGAGAGCAGG 
AGAAAGGAGG AGCAGCTAAA TAAATGGCTA ACAGCTCTGG CCAAAGACAG AGGGCCTGCC 
AACTTTGAGA GACACCGCCT TAGAGAGGGC TCAGACCATC AGGAGAGGAC AACCAAGAGA 
GGGAGATGTG AGGAAGACAG AGGCCCATCG CCCCATCTGT CTTCACACTC TCCTCCAAAC 
CCTCACAGAC TGTCCTCATC ACCACCCTCA TCCTCCACCT CTTCTTCTAA TTCAGACTCA 
GAATCTGAAT TTCTTATTTC AACCGCCAAA GTTTCAGCAG ATTCCATCTC TCACAAGACA 
TTCAGCAAGA AAGGGCAACA AGTCCCCAGC AGACCCAACG CCCCGAGGCC CCAGGCGGTT 
CACTCCAGAG GACCAAACTC GGGCCCTCCT TGTGAGGGGC AGCAAAGTGA GAGGAAGCAG 
AAACTCTACA CCTTGGTTCC ATTTGGACGA AGCGAGAAGG CAGCTCCCAC CTCCCAGCGG 
GGGCTGAGAA ACCTGGTGGT GCACATTGAC CTCTGTCTTC TAAAAAGGGT CCCTGATAGC 
TCAACAAATT CCCCTGTAAA GAAAAACACT TCTTCCTCAT CTTCTTCATC AACCAAGGAC 
AGGCAAAGAG AAGTTATGAA ACACACGCAC GTTCCAGATG CTTCGACAAA AGATGGCAAA 
AGGAAACGCA AGGTGGAGAA CGGAGTGTCA GACCGGGAAT GCAAGAGGAG TCAGCTTTAT 
CCAGATGACC CCCCTGGACC AACAGAATCA TCAGTCACAG CTGAGACTTT AGTGACTGAG 
ACGATGCACA ATGGGTACCT GGAGGAGTAC TTGGACAAGA GGCCAATGTC TCCCTTGTCT 
CCATTGTCTC CATTGTCTCC GCTGCCCCAG AGCCCTGAAC CCACCAAACC TACCTCCAAA 
ACAAAGACAA CTGAGCAGCA GAACTCTCGC ACCCAGAAAA GCAAGGAGAA GAGCAGAGAC 
CAGCCCGTCA AGCCCAAGAT GGAGGTGGAG TGTGTAAAAG TATCAAAACA ACCCCAACCT 
TCGTCCGAGT CCTCCTGGAG ACCTGCTGAC CACAGAGGAG CTGTTCCGCA TGAAACTTCT 
CACCATGCTG AGTACTACTT ACATGAAGCT AAAAGGATGA AGCACCGTGC CGATGCAATG 
GTGGATAAGT TGGGAAAAGC TGTTAACTAT GTTGATGCTG CTCTGTCCTT CATGGAATGT 
GGGAAGGCGA TGGAGGAAGG GCCGCTGGAG GCCAAGTCTC CTTACACCAT GTATTCAGAG 
ACGGTGGAGC TGATCAGGTA CGCCATGAAG CTGAAGAGCC ACTCGGGCCC TGGAGCAAGG 
CAGGAAGACA AACAGCTGGC GGTTCTGTGT TTCCGATGCC TTGCTCTCCT TTACTGGCAG 
ATGTTCCGTT TGAAAAAGGA TCATGCGCTG AAATACTCCA AAGTGCTGCT TGACTATTTC 
AAGAGTTCTC CCAAAGTGCC TACAACACCA CCTGGTTGGA ATGATTGTGG GAAGAGCATG 
GGAGAACACC CGTCTTCACT CTCACCCAAC GCCAGACACC GCAGAAGGGA CCCACCTGGT 
GGCAGCAGCT CATCTCTCAT AAGCATTCCC CAACGCATCC ACCAGATGGC AGCAAACCAC 
CTGAACATCA CCAACAGTGT CCTGTACAGT TATGAGTACT GGGAGGTAGC AGACAACCTT 
GCAAAGGAAA ATAAAGAGTT CTTCAACTAC TTGAATACCT TGTCTGGACC TTTGACTATT 
CACAGCAGCG TTGCTCATGC TGTCCAGTAC ACCAGACAAG CTCTTCAGTG GATACGCATC 
AGTGCCAAAC TTAATTAA