gspt1 cDNA ORF clone, Nothobranchius furzeri(turquoise killifish)

Gene
Clones
gRNAs

Summary

Gene Symbol	gspt1
Entrez Gene ID	107388241
Full Name	G1 to S phase transition 1
Gene Type	protein-coding
Organism	Nothobranchius furzeri(turquoise killifish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015963684.1	XP_015819170.1	eukaryotic peptide chain release factor GTP-binding subunit ERF3A

Pathways from BioSystems
Gene Ontology

The following gspt1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the gspt1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ONo20196	XM_015963684.1 Latest version!	Nothobranchius furzeri G1 to S phase transition 1 (gspt1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

gspt1 ( XM_015963684.1 ) cDNA ORF clone, Nothobranchius furzeri(turquoise killifish) -> XP_015819170.1 Nothobranchius furzeri G1 to S phase transition 1 (gspt1), mRNA.

Price & Availability↑

CloneID	ONo20196
Clone ID Related Accession (Same CDS sequence)	XM_015963684.1
Accession Version	XM_015963684.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1764bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-03-17
Organism	Nothobranchius furzeri(turquoise killifish)
Product	eukaryotic peptide chain release factor GTP-binding subunit ERF3A
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015443572.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Nothobranchius furzeri Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCCGA GAGACACAGC CCCGGATTCG TGGGAGCAGG AGGAAGATGC CGAGGCCCCG 
GCTCCGGCGG CGGACCTCCC GGCTGCCTTC GCTTCACTCA ACGTAAACGC CAAGCCTTTT 
GTCCCCAACG TTAACGCTCC GGTGTTTGTA CCGAGCTTTC TTCAAGCCAG CCCCGGGGAA 
ACGCCGTCTC CAGACGGTAC CCCTGACCCA GCTGCTATCA TGGAGGTGGC AGACCCAGTC 
GCTCCAGTAG AGAATGGAAA CACAGAGGCG GACATGACCG CTGAGGAGGA AACGTGGGAA 
CAGAAGGAGG AGCCTGGGGG AGGCGAGGGA GGCGGGGGAC ATGAGGACCA GAGGGAGATG 
GGAGATGGCT GTGAGGAGGG AGGTGCCACG GGGAAGGAAG ACGAGGAGAT GATGGAGGAG 
GAAGAGGAGG AGATGCCTAT GCCTAAAGTG GCTCCTCCAC CTCCTGATGC ACCCAAGAAA 
GAACACGTGA ACGTGGTCTT TATTGGTCAC GTGGATGCAG GAAAGTCCAC TATCGGGGGA 
CAGATTATGT ACCTCACGGG GATGGTAGAC AAGAGAACTC TGGAAAAATA CGAACGAGAA 
GCTAAAGAAA AGAACAGAGA GACCTGGTAT TTATCGTGGG CTCTGGACAC AAATCAGGAG 
GAGAGAGACA AGGGCAAGAC GGTGGAGGTT GGAAGAGCTT ATTTTGAGAC AGAGAAGAAA 
CACTTTACCA TTCTGGACGC CCCCGGACAC AAGAGCTTCG TCCCCAACAT GATCGGCGGG 
GCGTCGCAAG CTGACCTCGC AGTTCTGGTT ATTTCAGCGA GGAAGGGTGA GTTTGAGACG 
GGATTTGAGA AGGGTGGGCA GACCCGGGAA CATGCCATGC TGGCCAAAAC CGCCGGAGTT 
AAACATCTCA TCGTTCTGAT CAACAAGATG GACGACCCCA CTGTGAACTG GAGTCTAGAA 
AGATATGAGG AATGTAAAGA GAAGCTGGTG CCATTTTTGA AAAAAGTCGG GTTCAACCCA 
AAGAAGGACA TCCATTTTAT GCCTTGTTCT GGACTCACGG GTGCCAACCT GAAGGAGCCC 
GTAGTCGAGT GCCCCTGGTA CACGGGTTTG CCATTTATTT CCCACTTGGA CAGTTTGCCA 
AACTTCAACC GATCCAGCGA TGGGCCTGTC AGACTTCCCA TTGTGGACAA GTACAAGGAC 
ATGGGCACCG TCATCCTGGG GAAGCTGGAG TCTGGCTCCA TCAGTAAAGC CCAGCAGCTG 
GTCATGATGC CCAACAGGCA CACAGTAGAA GTGTTGAGCC TGCTTAGTGA TGACGTGGAG 
ACAGACGACG CGAGTCCAGG AGAGAACCTG AAGCTGAGGT TGAAGGGAAT AGAAGAAGAA 
GAAATCCTTC CAGGTTTCAT CCTCTGCAGC CCTGATAACC TCTGCCACTC GGGTCGCACG 
TTTGATGCCC AGATCGTCAT CATTGAGCAC AAATCGATCA TCTGTCCAGG ATATAACGCC 
GTTCTTCACA TCCACACCTG CATTGAGGAG GTGCAAATCA CGGCATTAAT CTGTCTGGTT 
GACAAGAAGA CGGGCGATAA GAGCAAAACA CGACCTCGCT TTGTGAAGCA GGATCAGGTG 
TGTATCGCCA GGCTCCGGGC AGCAGGAGTC ATCTGCTTGG AGACCTTCAA AGACTTCCCT 
CAGATGGGAC GGTTCACGCT GAGAGACGAA GGCAAAACCA TCGCCATCGG CAAAGTGCTG 
AAGCTGGTTC CAGAAAAGGA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015819170.1
CDS	212..1975
Translation	MDPRDTAPDSWEQEEDAEAPAPAADLPAAFASLNVNAKPFVPNVNAPVFVPSFLQASPGETPSPDGTPDPAAIMEVADPVAPVENGNTEADMTAEEETWEQKEEPGGGEGGGGHEDQREMGDGCEEGGATGKEDEEMMEEEEEEMPMPKVAPPPPDAPKKEHVNVVFIGHVDAGKSTIGGQIMYLTGMVDKRTLEKYEREAKEKNRETWYLSWALDTNQEERDKGKTVEVGRAYFETEKKHFTILDAPGHKSFVPNMIGGASQADLAVLVISARKGEFETGFEKGGQTREHAMLAKTAGVKHLIVLINKMDDPTVNWSLERYEECKEKLVPFLKKVGFNPKKDIHFMPCSGLTGANLKEPVVECPWYTGLPFISHLDSLPNFNRSSDGPVRLPIVDKYKDMGTVILGKLESGSISKAQQLVMMPNRHTVEVLSLLSDDVETDDASPGENLKLRLKGIEEEEILPGFILCSPDNLCHSGRTFDAQIVIIEHKSIICPGYNAVLHIHTCIEEVQITALICLVDKKTGDKSKTRPRFVKQDQVCIARLRAAGVICLETFKDFPQMGRFTLRDEGKTIAIGKVLKLVPEKD

Target ORF information:

RefSeq Version	XM_015963684.1
Organism	Nothobranchius furzeri(turquoise killifish)
Definition	Nothobranchius furzeri G1 to S phase transition 1 (gspt1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015963684.1

ORF Insert Sequence:

ATGGACCCGA GAGACACAGC CCCGGATTCG TGGGAGCAGG AGGAAGATGC CGAGGCCCCG 
GCTCCGGCGG CGGACCTCCC GGCTGCCTTC GCTTCACTCA ACGTAAACGC CAAGCCTTTT 
GTCCCCAACG TTAACGCTCC GGTGTTTGTA CCGAGCTTTC TTCAAGCCAG CCCCGGGGAA 
ACGCCGTCTC CAGACGGTAC CCCTGACCCA GCTGCTATCA TGGAGGTGGC AGACCCAGTC 
GCTCCAGTAG AGAATGGAAA CACAGAGGCG GACATGACCG CTGAGGAGGA AACGTGGGAA 
CAGAAGGAGG AGCCTGGGGG AGGCGAGGGA GGCGGGGGAC ATGAGGACCA GAGGGAGATG 
GGAGATGGCT GTGAGGAGGG AGGTGCCACG GGGAAGGAAG ACGAGGAGAT GATGGAGGAG 
GAAGAGGAGG AGATGCCTAT GCCTAAAGTG GCTCCTCCAC CTCCTGATGC ACCCAAGAAA 
GAACACGTGA ACGTGGTCTT TATTGGTCAC GTGGATGCAG GAAAGTCCAC TATCGGGGGA 
CAGATTATGT ACCTCACGGG GATGGTAGAC AAGAGAACTC TGGAAAAATA CGAACGAGAA 
GCTAAAGAAA AGAACAGAGA GACCTGGTAT TTATCGTGGG CTCTGGACAC AAATCAGGAG 
GAGAGAGACA AGGGCAAGAC GGTGGAGGTT GGAAGAGCTT ATTTTGAGAC AGAGAAGAAA 
CACTTTACCA TTCTGGACGC CCCCGGACAC AAGAGCTTCG TCCCCAACAT GATCGGCGGG 
GCGTCGCAAG CTGACCTCGC AGTTCTGGTT ATTTCAGCGA GGAAGGGTGA GTTTGAGACG 
GGATTTGAGA AGGGTGGGCA GACCCGGGAA CATGCCATGC TGGCCAAAAC CGCCGGAGTT 
AAACATCTCA TCGTTCTGAT CAACAAGATG GACGACCCCA CTGTGAACTG GAGTCTAGAA 
AGATATGAGG AATGTAAAGA GAAGCTGGTG CCATTTTTGA AAAAAGTCGG GTTCAACCCA 
AAGAAGGACA TCCATTTTAT GCCTTGTTCT GGACTCACGG GTGCCAACCT GAAGGAGCCC 
GTAGTCGAGT GCCCCTGGTA CACGGGTTTG CCATTTATTT CCCACTTGGA CAGTTTGCCA 
AACTTCAACC GATCCAGCGA TGGGCCTGTC AGACTTCCCA TTGTGGACAA GTACAAGGAC 
ATGGGCACCG TCATCCTGGG GAAGCTGGAG TCTGGCTCCA TCAGTAAAGC CCAGCAGCTG 
GTCATGATGC CCAACAGGCA CACAGTAGAA GTGTTGAGCC TGCTTAGTGA TGACGTGGAG 
ACAGACGACG CGAGTCCAGG AGAGAACCTG AAGCTGAGGT TGAAGGGAAT AGAAGAAGAA 
GAAATCCTTC CAGGTTTCAT CCTCTGCAGC CCTGATAACC TCTGCCACTC GGGTCGCACG 
TTTGATGCCC AGATCGTCAT CATTGAGCAC AAATCGATCA TCTGTCCAGG ATATAACGCC 
GTTCTTCACA TCCACACCTG CATTGAGGAG GTGCAAATCA CGGCATTAAT CTGTCTGGTT 
GACAAGAAGA CGGGCGATAA GAGCAAAACA CGACCTCGCT TTGTGAAGCA GGATCAGGTG 
TGTATCGCCA GGCTCCGGGC AGCAGGAGTC ATCTGCTTGG AGACCTTCAA AGACTTCCCT 
CAGATGGGAC GGTTCACGCT GAGAGACGAA GGCAAAACCA TCGCCATCGG CAAAGTGCTG 
AAGCTGGTTC CAGAAAAGGA CTAA