arhgef12 cDNA ORF clone, Nothobranchius furzeri(turquoise killifish)

Gene
Clones
gRNAs

Summary

Gene Symbol	arhgef12
Entrez Gene ID	107380349
Full Name	Rho guanine nucleotide exchange factor 12
Gene Type	protein-coding
Organism	Nothobranchius furzeri(turquoise killifish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015951561.1	XP_015807047.1	rho guanine nucleotide exchange factor 12 isoform X4
XM_015951558.1	XP_015807044.1	rho guanine nucleotide exchange factor 12 isoform X1
XM_015951562.1	XP_015807048.1	rho guanine nucleotide exchange factor 12 isoform X5
XM_015951559.1	XP_015807045.1	rho guanine nucleotide exchange factor 12 isoform X2
XM_015951560.1	XP_015807046.1	rho guanine nucleotide exchange factor 12 isoform X3
XM_015951563.1	XP_015807049.1	rho guanine nucleotide exchange factor 12 isoform X6

Pathways from BioSystems
Gene Ontology

The following arhgef12 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the arhgef12 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ONo10370	XM_015951558.1 Latest version!	Nothobranchius furzeri Rho guanine nucleotide exchange factor 12 (arhgef12), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
ONo10371	XM_015951559.1 Latest version!	Nothobranchius furzeri Rho guanine nucleotide exchange factor 12 (arhgef12), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
ONo10372	XM_015951560.1 Latest version!	Nothobranchius furzeri Rho guanine nucleotide exchange factor 12 (arhgef12), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
ONo10373	XM_015951561.1 Latest version!	Nothobranchius furzeri Rho guanine nucleotide exchange factor 12 (arhgef12), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
ONo10374	XM_015951562.1 Latest version!	Nothobranchius furzeri Rho guanine nucleotide exchange factor 12 (arhgef12), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
ONo10375	XM_015951563.1 Latest version!	Nothobranchius furzeri Rho guanine nucleotide exchange factor 12 (arhgef12), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

arhgef12 ( XM_015951558.1 ) cDNA ORF clone, Nothobranchius furzeri(turquoise killifish) -> XP_015807044.1 Nothobranchius furzeri Rho guanine nucleotide exchange factor 12 (arhgef12), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ONo10370
Clone ID Related Accession (Same CDS sequence)	XM_015951558.1
Accession Version	XM_015951558.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4281bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-03-17
Organism	Nothobranchius furzeri(turquoise killifish)
Product	rho guanine nucleotide exchange factor 12 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015443528.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Nothobranchius furzeri Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGACA CACAGCCCAG CTTCACTGAC AGGTTTAACA AGAAAGCAAT CAGAACCAGC 
AGCATTCTCA GTAAAGACCA CCCCCCAGAC AAGAAACCCA AAAGTGACAA AACCTCACCT 
CCCTCCAATG AGTTTGACCC TACAGACGCT CTGGTGGTGC AGAAGAGTGG CAACAACAGT 
GTGCTAGCAG AGGGGAAGCC TGAGTCCTGT GGTCTAGTCC AGCGATGTGT GATCATCCAG 
AAAGATGAAA ATGGCTTTGG GTTCACTGTC AGTGGCGATA ACCCCGTGTT TGTGCAGCTG 
GTCAAAGAAG ATGGGGCTGC TATGCGGGCA GGTGTTCAGA CAGGAGACAG GATCATCAAG 
GTTAACGGGA CCCTTGTGAC ACAGTCCAAT CACACTGAAG TTGTGAAACT TATCAAATCG 
GGCTCGTATG TGGCCCTCAC GGTTTTGGGG CGACCTCCGG GGCTCGCTCA GATCCCGTTG 
CCTGAGGCAG AGCCTGATGT GTTCGGCGTT AGGGTATCCT CCCCGAACTC TCCAGCCACC 
GAACGCTCAT GCAGTCCTCA GGACCGCCTC TTTTCCCCAC AACAACAATA TGACGAGAAC 
AACAGTTTCT GCGACCAGAG GACAAATGTC TTTGCAAAGT TTCTATCAAA GGAGCAGCAG 
GAGCTGCAGG TCATGAAAAA GGAGTACAGC AGGAACCCCT CCCCCAAACT ACTGAAAGAC 
ATTCAGGAAG CTACAAAACA CATTCCACAG CTGCAGGGTC AGCTCTTCAA GGCCACTGGG 
ACAACACAGG AGGCTGTTTC CGGTGGTGAT GAAGACAATG GTAGCATATC AGAGACAGAT 
TGGACCCCTC CTCATAAAGG AAACAACTGC ATAGACCTGC TGTGGAACAA CACTCCAAAA 
TCCCGTGTAT TCGAACCTGG ATCTCCTTCT ACCCAGAGTC CAAGAGACTC CTGCGCGAGC 
CCCAAAAGCT CTCCTAAAGA CAGCTTCAAC CCCGTCTACT CCCCAGAGAC GGAGGACGTC 
ACTGACCTGG ACACCGTCTC TCCCACCACG GTCAGCAGTC CTCTTCCCTG CCGCCTTGGC 
TTGCAAATTA TAGGAGCAGA AGACGACTAT TTTGATTCGG ACCAGGAGCA GACAAATGGC 
CAGTGTAACA GCTTTAACAG TATTGAGCAG CTCAAGTCTC GTCCTGCCCA CCTCGCAGCT 
TTTTTGCATC ATGTCATTTC TCAGTTTGAC CCTGCTCCTG TGTTGTGCTA CCTCTATGCT 
GACTATTACA AGCAAACCAA CTCCAAAGAA ACAAGAAAGG AGCTCCATAA CGTCTTCATA 
GACCGGGCAG CAATTTTAAA AGTTCCCATT CCTGAATCAG TTTCTTCTGA CCTTAACTGG 
CGACGAATGG AACTGAACCC TGAAGAAACA AACAAACAGT ATGTTAAAAC ACTGCAGGAC 
TCTCTCCTCT CTGACATTCA GAAAAACCTC GAGGACTTCA GGCAGAAACG CAGTATGGGC 
CTAACAGTGG CTAAGGCTGA GCTGACAAGA CTGGACCAAG AAAGAGTCAG AGACCGGGTC 
ACCATAGAGC GGGAGCGATC ATACGCAGAA AGCATCATCA GTAAAATAGA TGACATCCTG 
TTCACTACTC AAACCACAGA GGAAGACAAA TGCACTACGA TACAGAATGT TATCTACACA 
TACATGAAGC ACCTTGGGGT GAGAGTCAAG GAACCTCGAA TTCTGGAGGC CAAACGGGGT 
CGGAAAAGCT TTTTACCTAA AATGATGCAG AAAGGCATAA AAACTGAGAA GGAAGGAGAA 
GACAAAGGCA GAAAAGTATT TATGTTGCCC CTGCGTCGGC CCAGCCGCAT TGACTCGTCG 
CCAGCCATTA AAGCCCTGGA CAGTCGTCCC AAGCCTCAGA AACAGCTGTC TCAGCCGACG 
CCTGTGACCA GCGAGCTAAT GGAAGTTGGT CCATTGAGAG GCAGCCAATC CAGCGATTGC 
AATGACCTCA ATCACTCCAT GTCTGTCACC ACCTCATCTC CAAACATCAA CACCCACTGC 
ACAGATGTGA GCCGAGACGT CGACATCGGT GGGACGTCAT CTGGGTCCTC CAGACTGAAC 
GACGGCCTTC AGACCGACCT GGACGTGTTC CCATTCCCGT CTTCACCACT GGATGTTTGT 
GATCAGCTCC AGGAGGACGA CAGTGACCGA GCCCATGAGG GAACTCCAAA GGCCACAAGA 
AGATTTGAAG GATTGATTGC AGCTGATGTC CAAAGTGAGG ACGATCAAGG GACAGACTCT 
GAGCACAATC CTCCAAACTG GCATCAGCGG GTGGGTCGAG AAGTCCTCGT AGGCCTCAGT 
CCTCATGAAA TCAAGCGTCA GGAAGTTATC AACGAGCTGT TTTACACTGA GCGCGCTCAT 
GTGCGGATGC TGAAAGTTTT GGACCTCGTC TTCTACCAAA AGCTTTCCAA AGATGCAATT 
CTTCCTCCTA CTGACATCAA AAATATCTTC ACCAACTTGG AGGAGGTTCT GCAGCTGCAT 
GTTTCAATAC TGGAGCAAAT GACAGCTGTC CGGAAGAGAA ACGAGTCTTC CGTCATTGAT 
CAAATAGGAG ATGACTTGCT CTCTTGGTTC AGCGGCGGCG AGGAGGAGAA GATCAAGCAG 
GCAGTGGGGA CGTTTTGCAG CAATCAGCCT TTTGCTCTAG AGATTATTAA AACCAAGCAG 
AAGAAAGACT CGAGGTTCAT TTCATTCATC CAGGAGGCTG AGAGTAACAG ACTGTGTCGC 
CGGCTGCAGC TCAAAGACCT CATTCCTGTA GAGATGCTCC GGCTCACCAA GTACCCGCTG 
CTACTGGACA ACATCGTCAA GTACACAGAC ATTGCTGTGG AAAAGCAGAA AGTCAAGCAG 
GCAGCAGAAT GCTGTAGAAG GATCCTCAGT CATGTCAACC AGGCTGTGAA GGAGTCTGAG 
GACAAACAGA AATTAGAAGA CTATCAGCGA AGATTGGACC TCTCCACTCT AAAGCAGACT 
GACAACCCTC TGATCGCTGA ACTAAAGAAT CTGGATCTAA CAAAGAGGAC GATGATTCAT 
GAGGGTTCAC TGTCGTGGAA AGTGAACAGG GACAAGACTA TTGAGTTGTT CACACTCCTC 
CTTGAGGACA TCCTCGTTCT GCTGCAGAAA CAGGATGAGC GTCTGATCCT TAAGTGTCAC 
AGCAAGAACC TGGCAGGTGC TGCAGATACC AAACACATCT ACAGCCCCAT CATCAAACTC 
AGCACTGTCC TCGTGCGCTC TGTTGCTACG GACAACAGGT CCTTTTTCGT CCTCTCTGTG 
TCTGAAAACG GTTCCCACAT CTACGAGCTA ATGGCGCCCA CTGTGACGGA TCAGAACACG 
TGGCAAAGTC TGATCTCCCA GAGAGCCGAT GCAATGAAAG TCAAGCCTCA CAGCATCATC 
CCGTTACCTC AGCCTGACGG GGAGAGAGAT GGAGCGGAGA TCATAACAGC TGGTACTTCC 
CGACTGGGTA AAGACCCGGA CCGCACATCC ACCAGCAGCA CTCAGTCCAC AGATAAAGAG 
AGAGGTCCAG CCTCTAGAGG TGCATTACAG ACTTCTCTAC CTGGTAGTAA TCCTTTTGAA 
GAAGTGAAGG CTGAGGAGGA GGAGGAGGAG GGTGGCTTTG TGGACCGAGT GCTTCCTTAC 
CAAGTAGCTG AAATCGACAG AGAAGGAGAC TCGTTTGATG TGTTTCCCTC TCGAGCAGAT 
GAAGCACTGA AAACATTGGC AGCATTAAAG CACGTGTTGG TGACCCAGCT GATGTCCCAG 
GAGGCAGCAG AACAAAGCAA ACGCTCCTCT GGAGTCCGTC TCCTCAGGAC TACGTCCCTG 
CGGACCCCGA CGGACAACCG CAGCAAGGTT CTGGTCCACA ACGGCTCCGA GAAAAGTAGC 
TCCAAAACAG AAGACCTGGC TCAGGACCTG GGGTCGTGGG ACACTGGATT CTTTGAGTCA 
CCTGAAGATT ACGGATGTTT GGTCCTAGAA GGTTACTGCG GCCCACGGGA GAGCAGCACA 
GATGACGACA TCCTGGCCTC AACCACAGGG AAGCAGCTGA ACACCTCACA AGTCTGCGCT 
GCCGACTCCG GCATCAATTT ACGATTTTCC TCCACGTCAC AGGTCGGCAG CCTCTCCACT 
TTCAGCAGGC AGGTGCTGTC GCACCTTAGA AATCTTCAGA CCAACCTCAA CTATCTAAAG 
GAGGTGGAGG CGAAGTACAA CAATCTAATA CACCAAAGGC CAGAGAGGTC GACAACAGAC 
AAGGATAAAG ATAAGAGATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015807044.1
CDS	1386..5666
Translation	MSDTQPSFTDRFNKKAIRTSSILSKDHPPDKKPKSDKTSPPSNEFDPTDALVVQKSGNNSVLAEGKPESCGLVQRCVIIQKDENGFGFTVSGDNPVFVQLVKEDGAAMRAGVQTGDRIIKVNGTLVTQSNHTEVVKLIKSGSYVALTVLGRPPGLAQIPLPEAEPDVFGVRVSSPNSPATERSCSPQDRLFSPQQQYDENNSFCDQRTNVFAKFLSKEQQELQVMKKEYSRNPSPKLLKDIQEATKHIPQLQGQLFKATGTTQEAVSGGDEDNGSISETDWTPPHKGNNCIDLLWNNTPKSRVFEPGSPSTQSPRDSCASPKSSPKDSFNPVYSPETEDVTDLDTVSPTTVSSPLPCRLGLQIIGAEDDYFDSDQEQTNGQCNSFNSIEQLKSRPAHLAAFLHHVISQFDPAPVLCYLYADYYKQTNSKETRKELHNVFIDRAAILKVPIPESVSSDLNWRRMELNPEETNKQYVKTLQDSLLSDIQKNLEDFRQKRSMGLTVAKAELTRLDQERVRDRVTIERERSYAESIISKIDDILFTTQTTEEDKCTTIQNVIYTYMKHLGVRVKEPRILEAKRGRKSFLPKMMQKGIKTEKEGEDKGRKVFMLPLRRPSRIDSSPAIKALDSRPKPQKQLSQPTPVTSELMEVGPLRGSQSSDCNDLNHSMSVTTSSPNINTHCTDVSRDVDIGGTSSGSSRLNDGLQTDLDVFPFPSSPLDVCDQLQEDDSDRAHEGTPKATRRFEGLIAADVQSEDDQGTDSEHNPPNWHQRVGREVLVGLSPHEIKRQEVINELFYTERAHVRMLKVLDLVFYQKLSKDAILPPTDIKNIFTNLEEVLQLHVSILEQMTAVRKRNESSVIDQIGDDLLSWFSGGEEEKIKQAVGTFCSNQPFALEIIKTKQKKDSRFISFIQEAESNRLCRRLQLKDLIPVEMLRLTKYPLLLDNIVKYTDIAVEKQKVKQAAECCRRILSHVNQAVKESEDKQKLEDYQRRLDLSTLKQTDNPLIAELKNLDLTKRTMIHEGSLSWKVNRDKTIELFTLLLEDILVLLQKQDERLILKCHSKNLAGAADTKHIYSPIIKLSTVLVRSVATDNRSFFVLSVSENGSHIYELMAPTVTDQNTWQSLISQRADAMKVKPHSIIPLPQPDGERDGAEIITAGTSRLGKDPDRTSTSSTQSTDKERGPASRGALQTSLPGSNPFEEVKAEEEEEEGGFVDRVLPYQVAEIDREGDSFDVFPSRADEALKTLAALKHVLVTQLMSQEAAEQSKRSSGVRLLRTTSLRTPTDNRSKVLVHNGSEKSSSKTEDLAQDLGSWDTGFFESPEDYGCLVLEGYCGPRESSTDDDILASTTGKQLNTSQVCAADSGINLRFSSTSQVGSLSTFSRQVLSHLRNLQTNLNYLKEVEAKYNNLIHQRPERSTTDKDKDKR

Target ORF information:

RefSeq Version	XM_015951558.1
Organism	Nothobranchius furzeri(turquoise killifish)
Definition	Nothobranchius furzeri Rho guanine nucleotide exchange factor 12 (arhgef12), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015951558.1

ORF Insert Sequence:

ATGAGTGACA CACAGCCCAG CTTCACTGAC AGGTTTAACA AGAAAGCAAT CAGAACCAGC 
AGCATTCTCA GTAAAGACCA CCCCCCAGAC AAGAAACCCA AAAGTGACAA AACCTCACCT 
CCCTCCAATG AGTTTGACCC TACAGACGCT CTGGTGGTGC AGAAGAGTGG CAACAACAGT 
GTGCTAGCAG AGGGGAAGCC TGAGTCCTGT GGTCTAGTCC AGCGATGTGT GATCATCCAG 
AAAGATGAAA ATGGCTTTGG GTTCACTGTC AGTGGCGATA ACCCCGTGTT TGTGCAGCTG 
GTCAAAGAAG ATGGGGCTGC TATGCGGGCA GGTGTTCAGA CAGGAGACAG GATCATCAAG 
GTTAACGGGA CCCTTGTGAC ACAGTCCAAT CACACTGAAG TTGTGAAACT TATCAAATCG 
GGCTCGTATG TGGCCCTCAC GGTTTTGGGG CGACCTCCGG GGCTCGCTCA GATCCCGTTG 
CCTGAGGCAG AGCCTGATGT GTTCGGCGTT AGGGTATCCT CCCCGAACTC TCCAGCCACC 
GAACGCTCAT GCAGTCCTCA GGACCGCCTC TTTTCCCCAC AACAACAATA TGACGAGAAC 
AACAGTTTCT GCGACCAGAG GACAAATGTC TTTGCAAAGT TTCTATCAAA GGAGCAGCAG 
GAGCTGCAGG TCATGAAAAA GGAGTACAGC AGGAACCCCT CCCCCAAACT ACTGAAAGAC 
ATTCAGGAAG CTACAAAACA CATTCCACAG CTGCAGGGTC AGCTCTTCAA GGCCACTGGG 
ACAACACAGG AGGCTGTTTC CGGTGGTGAT GAAGACAATG GTAGCATATC AGAGACAGAT 
TGGACCCCTC CTCATAAAGG AAACAACTGC ATAGACCTGC TGTGGAACAA CACTCCAAAA 
TCCCGTGTAT TCGAACCTGG ATCTCCTTCT ACCCAGAGTC CAAGAGACTC CTGCGCGAGC 
CCCAAAAGCT CTCCTAAAGA CAGCTTCAAC CCCGTCTACT CCCCAGAGAC GGAGGACGTC 
ACTGACCTGG ACACCGTCTC TCCCACCACG GTCAGCAGTC CTCTTCCCTG CCGCCTTGGC 
TTGCAAATTA TAGGAGCAGA AGACGACTAT TTTGATTCGG ACCAGGAGCA GACAAATGGC 
CAGTGTAACA GCTTTAACAG TATTGAGCAG CTCAAGTCTC GTCCTGCCCA CCTCGCAGCT 
TTTTTGCATC ATGTCATTTC TCAGTTTGAC CCTGCTCCTG TGTTGTGCTA CCTCTATGCT 
GACTATTACA AGCAAACCAA CTCCAAAGAA ACAAGAAAGG AGCTCCATAA CGTCTTCATA 
GACCGGGCAG CAATTTTAAA AGTTCCCATT CCTGAATCAG TTTCTTCTGA CCTTAACTGG 
CGACGAATGG AACTGAACCC TGAAGAAACA AACAAACAGT ATGTTAAAAC ACTGCAGGAC 
TCTCTCCTCT CTGACATTCA GAAAAACCTC GAGGACTTCA GGCAGAAACG CAGTATGGGC 
CTAACAGTGG CTAAGGCTGA GCTGACAAGA CTGGACCAAG AAAGAGTCAG AGACCGGGTC 
ACCATAGAGC GGGAGCGATC ATACGCAGAA AGCATCATCA GTAAAATAGA TGACATCCTG 
TTCACTACTC AAACCACAGA GGAAGACAAA TGCACTACGA TACAGAATGT TATCTACACA 
TACATGAAGC ACCTTGGGGT GAGAGTCAAG GAACCTCGAA TTCTGGAGGC CAAACGGGGT 
CGGAAAAGCT TTTTACCTAA AATGATGCAG AAAGGCATAA AAACTGAGAA GGAAGGAGAA 
GACAAAGGCA GAAAAGTATT TATGTTGCCC CTGCGTCGGC CCAGCCGCAT TGACTCGTCG 
CCAGCCATTA AAGCCCTGGA CAGTCGTCCC AAGCCTCAGA AACAGCTGTC TCAGCCGACG 
CCTGTGACCA GCGAGCTAAT GGAAGTTGGT CCATTGAGAG GCAGCCAATC CAGCGATTGC 
AATGACCTCA ATCACTCCAT GTCTGTCACC ACCTCATCTC CAAACATCAA CACCCACTGC 
ACAGATGTGA GCCGAGACGT CGACATCGGT GGGACGTCAT CTGGGTCCTC CAGACTGAAC 
GACGGCCTTC AGACCGACCT GGACGTGTTC CCATTCCCGT CTTCACCACT GGATGTTTGT 
GATCAGCTCC AGGAGGACGA CAGTGACCGA GCCCATGAGG GAACTCCAAA GGCCACAAGA 
AGATTTGAAG GATTGATTGC AGCTGATGTC CAAAGTGAGG ACGATCAAGG GACAGACTCT 
GAGCACAATC CTCCAAACTG GCATCAGCGG GTGGGTCGAG AAGTCCTCGT AGGCCTCAGT 
CCTCATGAAA TCAAGCGTCA GGAAGTTATC AACGAGCTGT TTTACACTGA GCGCGCTCAT 
GTGCGGATGC TGAAAGTTTT GGACCTCGTC TTCTACCAAA AGCTTTCCAA AGATGCAATT 
CTTCCTCCTA CTGACATCAA AAATATCTTC ACCAACTTGG AGGAGGTTCT GCAGCTGCAT 
GTTTCAATAC TGGAGCAAAT GACAGCTGTC CGGAAGAGAA ACGAGTCTTC CGTCATTGAT 
CAAATAGGAG ATGACTTGCT CTCTTGGTTC AGCGGCGGCG AGGAGGAGAA GATCAAGCAG 
GCAGTGGGGA CGTTTTGCAG CAATCAGCCT TTTGCTCTAG AGATTATTAA AACCAAGCAG 
AAGAAAGACT CGAGGTTCAT TTCATTCATC CAGGAGGCTG AGAGTAACAG ACTGTGTCGC 
CGGCTGCAGC TCAAAGACCT CATTCCTGTA GAGATGCTCC GGCTCACCAA GTACCCGCTG 
CTACTGGACA ACATCGTCAA GTACACAGAC ATTGCTGTGG AAAAGCAGAA AGTCAAGCAG 
GCAGCAGAAT GCTGTAGAAG GATCCTCAGT CATGTCAACC AGGCTGTGAA GGAGTCTGAG 
GACAAACAGA AATTAGAAGA CTATCAGCGA AGATTGGACC TCTCCACTCT AAAGCAGACT 
GACAACCCTC TGATCGCTGA ACTAAAGAAT CTGGATCTAA CAAAGAGGAC GATGATTCAT 
GAGGGTTCAC TGTCGTGGAA AGTGAACAGG GACAAGACTA TTGAGTTGTT CACACTCCTC 
CTTGAGGACA TCCTCGTTCT GCTGCAGAAA CAGGATGAGC GTCTGATCCT TAAGTGTCAC 
AGCAAGAACC TGGCAGGTGC TGCAGATACC AAACACATCT ACAGCCCCAT CATCAAACTC 
AGCACTGTCC TCGTGCGCTC TGTTGCTACG GACAACAGGT CCTTTTTCGT CCTCTCTGTG 
TCTGAAAACG GTTCCCACAT CTACGAGCTA ATGGCGCCCA CTGTGACGGA TCAGAACACG 
TGGCAAAGTC TGATCTCCCA GAGAGCCGAT GCAATGAAAG TCAAGCCTCA CAGCATCATC 
CCGTTACCTC AGCCTGACGG GGAGAGAGAT GGAGCGGAGA TCATAACAGC TGGTACTTCC 
CGACTGGGTA AAGACCCGGA CCGCACATCC ACCAGCAGCA CTCAGTCCAC AGATAAAGAG 
AGAGGTCCAG CCTCTAGAGG TGCATTACAG ACTTCTCTAC CTGGTAGTAA TCCTTTTGAA 
GAAGTGAAGG CTGAGGAGGA GGAGGAGGAG GGTGGCTTTG TGGACCGAGT GCTTCCTTAC 
CAAGTAGCTG AAATCGACAG AGAAGGAGAC TCGTTTGATG TGTTTCCCTC TCGAGCAGAT 
GAAGCACTGA AAACATTGGC AGCATTAAAG CACGTGTTGG TGACCCAGCT GATGTCCCAG 
GAGGCAGCAG AACAAAGCAA ACGCTCCTCT GGAGTCCGTC TCCTCAGGAC TACGTCCCTG 
CGGACCCCGA CGGACAACCG CAGCAAGGTT CTGGTCCACA ACGGCTCCGA GAAAAGTAGC 
TCCAAAACAG AAGACCTGGC TCAGGACCTG GGGTCGTGGG ACACTGGATT CTTTGAGTCA 
CCTGAAGATT ACGGATGTTT GGTCCTAGAA GGTTACTGCG GCCCACGGGA GAGCAGCACA 
GATGACGACA TCCTGGCCTC AACCACAGGG AAGCAGCTGA ACACCTCACA AGTCTGCGCT 
GCCGACTCCG GCATCAATTT ACGATTTTCC TCCACGTCAC AGGTCGGCAG CCTCTCCACT 
TTCAGCAGGC AGGTGCTGTC GCACCTTAGA AATCTTCAGA CCAACCTCAA CTATCTAAAG 
GAGGTGGAGG CGAAGTACAA CAATCTAATA CACCAAAGGC CAGAGAGGTC GACAACAGAC 
AAGGATAAAG ATAAGAGATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

arhgef12 ( XM_015951559.1 ) cDNA ORF clone, Nothobranchius furzeri(turquoise killifish) -> XP_015807045.1 Nothobranchius furzeri Rho guanine nucleotide exchange factor 12 (arhgef12), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ONo10371
Clone ID Related Accession (Same CDS sequence)	XM_015951559.1
Accession Version	XM_015951559.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4278bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-03-17
Organism	Nothobranchius furzeri(turquoise killifish)
Product	rho guanine nucleotide exchange factor 12 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015443528.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Nothobranchius furzeri Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGACA CACAGCCCAG CTTCACTGAC AGGTTTAACA AGAAAGCAAT CAGAACCAGC 
AGCATTCTCA GTAAAGACCA CCCCCCAGAC AAGAAACCCA AAAGTGACAA AACCTCACCT 
CCCTCCAATG AGTTTGACCC TACAGACGCT CTGGTGGTGC AGAAGAGTGG CAACAACAGT 
GTGCTAGCAG AGGGGAAGCC TGAGTCCTGT GGTCTAGTCC AGCGATGTGT GATCATCCAG 
AAAGATGAAA ATGGCTTTGG GTTCACTGTC AGTGGCGATA ACCCCGTGTT TGTGCAGCTG 
GTCAAAGAAG ATGGGGCTGC TATGCGGGCA GGTGTTCAGA CAGGAGACAG GATCATCAAG 
GTTAACGGGA CCCTTGTGAC ACAGTCCAAT CACACTGAAG TTGTGAAACT TATCAAATCG 
GGCTCGTATG TGGCCCTCAC GGTTTTGGGG CGACCTCCGG GGCTCGCTCA GATCCCGTTG 
CCTGAGGCAG AGCCTGATGT GTTCGGCGTT AGGGTATCCT CCCCGAACTC TCCAGCCACC 
GAACGCTCAT GCAGTCCTCA GGACCGCCTC TTTTCCCCAC AACAACAATA TGACGAGAAC 
AACAGTTTCT GCGACCAGAG GACAAATGTC TTTGCAAAGT TTCTATCAAA GGAGCAGCAG 
GAGCTGCAGG TCATGAAAAA GGAGTACAGC AGGAACCCCT CCCCCAAACT ACTGAAAGAC 
ATTCAGGAAG CTACAAAACA CATTCCACAG CTGCAGGGTC AGCTCTTCAA GGCCACTGGG 
ACAACACAGG AGGCTGTTTC CGGTGGTGAT GAAGACAATG GTAGCATATC AGAGACAGAT 
TGGACCCCTC CTCATAAAGG AAACAACTGC ATAGACCTGC TGTGGAACAA CACTCCAAAA 
TCCCGTGTAT TCGAACCTGG ATCTCCTTCT ACCCAGAGTC CAAGAGACTC CTGCGCGAGC 
CCCAAAAGCT CTCCTAAAGA CAGCTTCAAC CCCGTCTACT CCCCAGAGAC GGAGGACGTC 
ACTGACCTGG ACACCGTCTC TCCCACCACG GTCAGCAGTC CTCTTCCCTG CCGCCTTGGC 
TTGCAAATTA TAGGAGCAGA AGACGACTAT TTTGATTCGG ACCAGGAGCA GACAAATGGC 
CAGTGTAACA GCTTTAACAG TATTGAGCAG CTCAAGTCTC GTCCTGCCCA CCTCGCAGCT 
TTTTTGCATC ATGTCATTTC TCAGTTTGAC CCTGCTCCTG TGTTGTGCTA CCTCTATGCT 
GACTATTACA AGCAAACCAA CTCCAAAGAA ACAAGAAAGG AGCTCCATAA CGTCTTCATA 
GACCGGGCAG CAATTTTAAA AGTTCCCATT CCTGAATCAG TTTCTTCTGA CCTTAACTGG 
CGACGAATGG AACTGAACCC TGAAGAAACA AACAAACAGT ATGTTAAAAC ACTGCAGGAC 
TCTCTCCTCT CTGACATTCA GAAAAACCTC GAGGACTTCA GGCAGAAACG CAGTATGGGC 
CTAACAGTGG CTAAGGCTGA GCTGACAAGA CTGGACCAAG AAAGAGTCAG AGACCGGGTC 
ACCATAGAGC GGGAGCGATC ATACGCAGAA AGCATCATCA GTAAAATAGA TGACATCCTG 
TTCACTACTC AAACCACAGA GGAAGACAAA TGCACTACGA TACAGAATGT TATCTACACA 
TACATGAAGC ACCTTGGGGT GAGAGTCAAG GAACCTCGAA TTCTGGAGGC CAAACGGGGT 
CGGAAAAGCT TTTTACCTAA AATGATGAAA GGCATAAAAA CTGAGAAGGA AGGAGAAGAC 
AAAGGCAGAA AAGTATTTAT GTTGCCCCTG CGTCGGCCCA GCCGCATTGA CTCGTCGCCA 
GCCATTAAAG CCCTGGACAG TCGTCCCAAG CCTCAGAAAC AGCTGTCTCA GCCGACGCCT 
GTGACCAGCG AGCTAATGGA AGTTGGTCCA TTGAGAGGCA GCCAATCCAG CGATTGCAAT 
GACCTCAATC ACTCCATGTC TGTCACCACC TCATCTCCAA ACATCAACAC CCACTGCACA 
GATGTGAGCC GAGACGTCGA CATCGGTGGG ACGTCATCTG GGTCCTCCAG ACTGAACGAC 
GGCCTTCAGA CCGACCTGGA CGTGTTCCCA TTCCCGTCTT CACCACTGGA TGTTTGTGAT 
CAGCTCCAGG AGGACGACAG TGACCGAGCC CATGAGGGAA CTCCAAAGGC CACAAGAAGA 
TTTGAAGGAT TGATTGCAGC TGATGTCCAA AGTGAGGACG ATCAAGGGAC AGACTCTGAG 
CACAATCCTC CAAACTGGCA TCAGCGGGTG GGTCGAGAAG TCCTCGTAGG CCTCAGTCCT 
CATGAAATCA AGCGTCAGGA AGTTATCAAC GAGCTGTTTT ACACTGAGCG CGCTCATGTG 
CGGATGCTGA AAGTTTTGGA CCTCGTCTTC TACCAAAAGC TTTCCAAAGA TGCAATTCTT 
CCTCCTACTG ACATCAAAAA TATCTTCACC AACTTGGAGG AGGTTCTGCA GCTGCATGTT 
TCAATACTGG AGCAAATGAC AGCTGTCCGG AAGAGAAACG AGTCTTCCGT CATTGATCAA 
ATAGGAGATG ACTTGCTCTC TTGGTTCAGC GGCGGCGAGG AGGAGAAGAT CAAGCAGGCA 
GTGGGGACGT TTTGCAGCAA TCAGCCTTTT GCTCTAGAGA TTATTAAAAC CAAGCAGAAG 
AAAGACTCGA GGTTCATTTC ATTCATCCAG GAGGCTGAGA GTAACAGACT GTGTCGCCGG 
CTGCAGCTCA AAGACCTCAT TCCTGTAGAG ATGCTCCGGC TCACCAAGTA CCCGCTGCTA 
CTGGACAACA TCGTCAAGTA CACAGACATT GCTGTGGAAA AGCAGAAAGT CAAGCAGGCA 
GCAGAATGCT GTAGAAGGAT CCTCAGTCAT GTCAACCAGG CTGTGAAGGA GTCTGAGGAC 
AAACAGAAAT TAGAAGACTA TCAGCGAAGA TTGGACCTCT CCACTCTAAA GCAGACTGAC 
AACCCTCTGA TCGCTGAACT AAAGAATCTG GATCTAACAA AGAGGACGAT GATTCATGAG 
GGTTCACTGT CGTGGAAAGT GAACAGGGAC AAGACTATTG AGTTGTTCAC ACTCCTCCTT 
GAGGACATCC TCGTTCTGCT GCAGAAACAG GATGAGCGTC TGATCCTTAA GTGTCACAGC 
AAGAACCTGG CAGGTGCTGC AGATACCAAA CACATCTACA GCCCCATCAT CAAACTCAGC 
ACTGTCCTCG TGCGCTCTGT TGCTACGGAC AACAGGTCCT TTTTCGTCCT CTCTGTGTCT 
GAAAACGGTT CCCACATCTA CGAGCTAATG GCGCCCACTG TGACGGATCA GAACACGTGG 
CAAAGTCTGA TCTCCCAGAG AGCCGATGCA ATGAAAGTCA AGCCTCACAG CATCATCCCG 
TTACCTCAGC CTGACGGGGA GAGAGATGGA GCGGAGATCA TAACAGCTGG TACTTCCCGA 
CTGGGTAAAG ACCCGGACCG CACATCCACC AGCAGCACTC AGTCCACAGA TAAAGAGAGA 
GGTCCAGCCT CTAGAGGTGC ATTACAGACT TCTCTACCTG GTAGTAATCC TTTTGAAGAA 
GTGAAGGCTG AGGAGGAGGA GGAGGAGGGT GGCTTTGTGG ACCGAGTGCT TCCTTACCAA 
GTAGCTGAAA TCGACAGAGA AGGAGACTCG TTTGATGTGT TTCCCTCTCG AGCAGATGAA 
GCACTGAAAA CATTGGCAGC ATTAAAGCAC GTGTTGGTGA CCCAGCTGAT GTCCCAGGAG 
GCAGCAGAAC AAAGCAAACG CTCCTCTGGA GTCCGTCTCC TCAGGACTAC GTCCCTGCGG 
ACCCCGACGG ACAACCGCAG CAAGGTTCTG GTCCACAACG GCTCCGAGAA AAGTAGCTCC 
AAAACAGAAG ACCTGGCTCA GGACCTGGGG TCGTGGGACA CTGGATTCTT TGAGTCACCT 
GAAGATTACG GATGTTTGGT CCTAGAAGGT TACTGCGGCC CACGGGAGAG CAGCACAGAT 
GACGACATCC TGGCCTCAAC CACAGGGAAG CAGCTGAACA CCTCACAAGT CTGCGCTGCC 
GACTCCGGCA TCAATTTACG ATTTTCCTCC ACGTCACAGG TCGGCAGCCT CTCCACTTTC 
AGCAGGCAGG TGCTGTCGCA CCTTAGAAAT CTTCAGACCA ACCTCAACTA TCTAAAGGAG 
GTGGAGGCGA AGTACAACAA TCTAATACAC CAAAGGCCAG AGAGGTCGAC AACAGACAAG 
GATAAAGATA AGAGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015807045.1
CDS	1386..5663
Translation	MSDTQPSFTDRFNKKAIRTSSILSKDHPPDKKPKSDKTSPPSNEFDPTDALVVQKSGNNSVLAEGKPESCGLVQRCVIIQKDENGFGFTVSGDNPVFVQLVKEDGAAMRAGVQTGDRIIKVNGTLVTQSNHTEVVKLIKSGSYVALTVLGRPPGLAQIPLPEAEPDVFGVRVSSPNSPATERSCSPQDRLFSPQQQYDENNSFCDQRTNVFAKFLSKEQQELQVMKKEYSRNPSPKLLKDIQEATKHIPQLQGQLFKATGTTQEAVSGGDEDNGSISETDWTPPHKGNNCIDLLWNNTPKSRVFEPGSPSTQSPRDSCASPKSSPKDSFNPVYSPETEDVTDLDTVSPTTVSSPLPCRLGLQIIGAEDDYFDSDQEQTNGQCNSFNSIEQLKSRPAHLAAFLHHVISQFDPAPVLCYLYADYYKQTNSKETRKELHNVFIDRAAILKVPIPESVSSDLNWRRMELNPEETNKQYVKTLQDSLLSDIQKNLEDFRQKRSMGLTVAKAELTRLDQERVRDRVTIERERSYAESIISKIDDILFTTQTTEEDKCTTIQNVIYTYMKHLGVRVKEPRILEAKRGRKSFLPKMMKGIKTEKEGEDKGRKVFMLPLRRPSRIDSSPAIKALDSRPKPQKQLSQPTPVTSELMEVGPLRGSQSSDCNDLNHSMSVTTSSPNINTHCTDVSRDVDIGGTSSGSSRLNDGLQTDLDVFPFPSSPLDVCDQLQEDDSDRAHEGTPKATRRFEGLIAADVQSEDDQGTDSEHNPPNWHQRVGREVLVGLSPHEIKRQEVINELFYTERAHVRMLKVLDLVFYQKLSKDAILPPTDIKNIFTNLEEVLQLHVSILEQMTAVRKRNESSVIDQIGDDLLSWFSGGEEEKIKQAVGTFCSNQPFALEIIKTKQKKDSRFISFIQEAESNRLCRRLQLKDLIPVEMLRLTKYPLLLDNIVKYTDIAVEKQKVKQAAECCRRILSHVNQAVKESEDKQKLEDYQRRLDLSTLKQTDNPLIAELKNLDLTKRTMIHEGSLSWKVNRDKTIELFTLLLEDILVLLQKQDERLILKCHSKNLAGAADTKHIYSPIIKLSTVLVRSVATDNRSFFVLSVSENGSHIYELMAPTVTDQNTWQSLISQRADAMKVKPHSIIPLPQPDGERDGAEIITAGTSRLGKDPDRTSTSSTQSTDKERGPASRGALQTSLPGSNPFEEVKAEEEEEEGGFVDRVLPYQVAEIDREGDSFDVFPSRADEALKTLAALKHVLVTQLMSQEAAEQSKRSSGVRLLRTTSLRTPTDNRSKVLVHNGSEKSSSKTEDLAQDLGSWDTGFFESPEDYGCLVLEGYCGPRESSTDDDILASTTGKQLNTSQVCAADSGINLRFSSTSQVGSLSTFSRQVLSHLRNLQTNLNYLKEVEAKYNNLIHQRPERSTTDKDKDKR

Target ORF information:

RefSeq Version	XM_015951559.1
Organism	Nothobranchius furzeri(turquoise killifish)
Definition	Nothobranchius furzeri Rho guanine nucleotide exchange factor 12 (arhgef12), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015951559.1

ORF Insert Sequence:

ATGAGTGACA CACAGCCCAG CTTCACTGAC AGGTTTAACA AGAAAGCAAT CAGAACCAGC 
AGCATTCTCA GTAAAGACCA CCCCCCAGAC AAGAAACCCA AAAGTGACAA AACCTCACCT 
CCCTCCAATG AGTTTGACCC TACAGACGCT CTGGTGGTGC AGAAGAGTGG CAACAACAGT 
GTGCTAGCAG AGGGGAAGCC TGAGTCCTGT GGTCTAGTCC AGCGATGTGT GATCATCCAG 
AAAGATGAAA ATGGCTTTGG GTTCACTGTC AGTGGCGATA ACCCCGTGTT TGTGCAGCTG 
GTCAAAGAAG ATGGGGCTGC TATGCGGGCA GGTGTTCAGA CAGGAGACAG GATCATCAAG 
GTTAACGGGA CCCTTGTGAC ACAGTCCAAT CACACTGAAG TTGTGAAACT TATCAAATCG 
GGCTCGTATG TGGCCCTCAC GGTTTTGGGG CGACCTCCGG GGCTCGCTCA GATCCCGTTG 
CCTGAGGCAG AGCCTGATGT GTTCGGCGTT AGGGTATCCT CCCCGAACTC TCCAGCCACC 
GAACGCTCAT GCAGTCCTCA GGACCGCCTC TTTTCCCCAC AACAACAATA TGACGAGAAC 
AACAGTTTCT GCGACCAGAG GACAAATGTC TTTGCAAAGT TTCTATCAAA GGAGCAGCAG 
GAGCTGCAGG TCATGAAAAA GGAGTACAGC AGGAACCCCT CCCCCAAACT ACTGAAAGAC 
ATTCAGGAAG CTACAAAACA CATTCCACAG CTGCAGGGTC AGCTCTTCAA GGCCACTGGG 
ACAACACAGG AGGCTGTTTC CGGTGGTGAT GAAGACAATG GTAGCATATC AGAGACAGAT 
TGGACCCCTC CTCATAAAGG AAACAACTGC ATAGACCTGC TGTGGAACAA CACTCCAAAA 
TCCCGTGTAT TCGAACCTGG ATCTCCTTCT ACCCAGAGTC CAAGAGACTC CTGCGCGAGC 
CCCAAAAGCT CTCCTAAAGA CAGCTTCAAC CCCGTCTACT CCCCAGAGAC GGAGGACGTC 
ACTGACCTGG ACACCGTCTC TCCCACCACG GTCAGCAGTC CTCTTCCCTG CCGCCTTGGC 
TTGCAAATTA TAGGAGCAGA AGACGACTAT TTTGATTCGG ACCAGGAGCA GACAAATGGC 
CAGTGTAACA GCTTTAACAG TATTGAGCAG CTCAAGTCTC GTCCTGCCCA CCTCGCAGCT 
TTTTTGCATC ATGTCATTTC TCAGTTTGAC CCTGCTCCTG TGTTGTGCTA CCTCTATGCT 
GACTATTACA AGCAAACCAA CTCCAAAGAA ACAAGAAAGG AGCTCCATAA CGTCTTCATA 
GACCGGGCAG CAATTTTAAA AGTTCCCATT CCTGAATCAG TTTCTTCTGA CCTTAACTGG 
CGACGAATGG AACTGAACCC TGAAGAAACA AACAAACAGT ATGTTAAAAC ACTGCAGGAC 
TCTCTCCTCT CTGACATTCA GAAAAACCTC GAGGACTTCA GGCAGAAACG CAGTATGGGC 
CTAACAGTGG CTAAGGCTGA GCTGACAAGA CTGGACCAAG AAAGAGTCAG AGACCGGGTC 
ACCATAGAGC GGGAGCGATC ATACGCAGAA AGCATCATCA GTAAAATAGA TGACATCCTG 
TTCACTACTC AAACCACAGA GGAAGACAAA TGCACTACGA TACAGAATGT TATCTACACA 
TACATGAAGC ACCTTGGGGT GAGAGTCAAG GAACCTCGAA TTCTGGAGGC CAAACGGGGT 
CGGAAAAGCT TTTTACCTAA AATGATGAAA GGCATAAAAA CTGAGAAGGA AGGAGAAGAC 
AAAGGCAGAA AAGTATTTAT GTTGCCCCTG CGTCGGCCCA GCCGCATTGA CTCGTCGCCA 
GCCATTAAAG CCCTGGACAG TCGTCCCAAG CCTCAGAAAC AGCTGTCTCA GCCGACGCCT 
GTGACCAGCG AGCTAATGGA AGTTGGTCCA TTGAGAGGCA GCCAATCCAG CGATTGCAAT 
GACCTCAATC ACTCCATGTC TGTCACCACC TCATCTCCAA ACATCAACAC CCACTGCACA 
GATGTGAGCC GAGACGTCGA CATCGGTGGG ACGTCATCTG GGTCCTCCAG ACTGAACGAC 
GGCCTTCAGA CCGACCTGGA CGTGTTCCCA TTCCCGTCTT CACCACTGGA TGTTTGTGAT 
CAGCTCCAGG AGGACGACAG TGACCGAGCC CATGAGGGAA CTCCAAAGGC CACAAGAAGA 
TTTGAAGGAT TGATTGCAGC TGATGTCCAA AGTGAGGACG ATCAAGGGAC AGACTCTGAG 
CACAATCCTC CAAACTGGCA TCAGCGGGTG GGTCGAGAAG TCCTCGTAGG CCTCAGTCCT 
CATGAAATCA AGCGTCAGGA AGTTATCAAC GAGCTGTTTT ACACTGAGCG CGCTCATGTG 
CGGATGCTGA AAGTTTTGGA CCTCGTCTTC TACCAAAAGC TTTCCAAAGA TGCAATTCTT 
CCTCCTACTG ACATCAAAAA TATCTTCACC AACTTGGAGG AGGTTCTGCA GCTGCATGTT 
TCAATACTGG AGCAAATGAC AGCTGTCCGG AAGAGAAACG AGTCTTCCGT CATTGATCAA 
ATAGGAGATG ACTTGCTCTC TTGGTTCAGC GGCGGCGAGG AGGAGAAGAT CAAGCAGGCA 
GTGGGGACGT TTTGCAGCAA TCAGCCTTTT GCTCTAGAGA TTATTAAAAC CAAGCAGAAG 
AAAGACTCGA GGTTCATTTC ATTCATCCAG GAGGCTGAGA GTAACAGACT GTGTCGCCGG 
CTGCAGCTCA AAGACCTCAT TCCTGTAGAG ATGCTCCGGC TCACCAAGTA CCCGCTGCTA 
CTGGACAACA TCGTCAAGTA CACAGACATT GCTGTGGAAA AGCAGAAAGT CAAGCAGGCA 
GCAGAATGCT GTAGAAGGAT CCTCAGTCAT GTCAACCAGG CTGTGAAGGA GTCTGAGGAC 
AAACAGAAAT TAGAAGACTA TCAGCGAAGA TTGGACCTCT CCACTCTAAA GCAGACTGAC 
AACCCTCTGA TCGCTGAACT AAAGAATCTG GATCTAACAA AGAGGACGAT GATTCATGAG 
GGTTCACTGT CGTGGAAAGT GAACAGGGAC AAGACTATTG AGTTGTTCAC ACTCCTCCTT 
GAGGACATCC TCGTTCTGCT GCAGAAACAG GATGAGCGTC TGATCCTTAA GTGTCACAGC 
AAGAACCTGG CAGGTGCTGC AGATACCAAA CACATCTACA GCCCCATCAT CAAACTCAGC 
ACTGTCCTCG TGCGCTCTGT TGCTACGGAC AACAGGTCCT TTTTCGTCCT CTCTGTGTCT 
GAAAACGGTT CCCACATCTA CGAGCTAATG GCGCCCACTG TGACGGATCA GAACACGTGG 
CAAAGTCTGA TCTCCCAGAG AGCCGATGCA ATGAAAGTCA AGCCTCACAG CATCATCCCG 
TTACCTCAGC CTGACGGGGA GAGAGATGGA GCGGAGATCA TAACAGCTGG TACTTCCCGA 
CTGGGTAAAG ACCCGGACCG CACATCCACC AGCAGCACTC AGTCCACAGA TAAAGAGAGA 
GGTCCAGCCT CTAGAGGTGC ATTACAGACT TCTCTACCTG GTAGTAATCC TTTTGAAGAA 
GTGAAGGCTG AGGAGGAGGA GGAGGAGGGT GGCTTTGTGG ACCGAGTGCT TCCTTACCAA 
GTAGCTGAAA TCGACAGAGA AGGAGACTCG TTTGATGTGT TTCCCTCTCG AGCAGATGAA 
GCACTGAAAA CATTGGCAGC ATTAAAGCAC GTGTTGGTGA CCCAGCTGAT GTCCCAGGAG 
GCAGCAGAAC AAAGCAAACG CTCCTCTGGA GTCCGTCTCC TCAGGACTAC GTCCCTGCGG 
ACCCCGACGG ACAACCGCAG CAAGGTTCTG GTCCACAACG GCTCCGAGAA AAGTAGCTCC 
AAAACAGAAG ACCTGGCTCA GGACCTGGGG TCGTGGGACA CTGGATTCTT TGAGTCACCT 
GAAGATTACG GATGTTTGGT CCTAGAAGGT TACTGCGGCC CACGGGAGAG CAGCACAGAT 
GACGACATCC TGGCCTCAAC CACAGGGAAG CAGCTGAACA CCTCACAAGT CTGCGCTGCC 
GACTCCGGCA TCAATTTACG ATTTTCCTCC ACGTCACAGG TCGGCAGCCT CTCCACTTTC 
AGCAGGCAGG TGCTGTCGCA CCTTAGAAAT CTTCAGACCA ACCTCAACTA TCTAAAGGAG 
GTGGAGGCGA AGTACAACAA TCTAATACAC CAAAGGCCAG AGAGGTCGAC AACAGACAAG 
GATAAAGATA AGAGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

arhgef12 ( XM_015951560.1 ) cDNA ORF clone, Nothobranchius furzeri(turquoise killifish) -> XP_015807046.1 Nothobranchius furzeri Rho guanine nucleotide exchange factor 12 (arhgef12), transcript variant X3, mRNA.

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CloneID	ONo10372
Clone ID Related Accession (Same CDS sequence)	XM_015951560.1
Accession Version	XM_015951560.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4266bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-03-17
Organism	Nothobranchius furzeri(turquoise killifish)
Product	rho guanine nucleotide exchange factor 12 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015443528.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Nothobranchius furzeri Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGACA CACAGCCCAG CTTCACTGAC AGGTTTAACA AGAAAGCAAT CAGAACCAGC 
AGCATTCTCA GTAAAGACCA CCCCCCAGAC AAGAAACCCA AAAGTGACAA AACCTCACCT 
CCCTCCAATG AGTTTGACCC TACAGACGCT CTGGTGGTGC AGAAGAGTGG CAACAACAGT 
GTGCTAGCAG AGGGGAAGCC TGAGTCCTGT GGTCTAGTCC AGCGATGTGT GATCATCCAG 
AAAGATGAAA ATGGCTTTGG GTTCACTGTC AGTGGCGATA ACCCCGTGTT TGTGCAGCTG 
GTCAAAGAAG ATGGGGCTGC TATGCGGGCA GGTGTTCAGA CAGGAGACAG GATCATCAAG 
GTTAACGGGA CCCTTGTGAC ACAGTCCAAT CACACTGAAG TTGTGAAACT TATCAAATCG 
GGCTCGTATG TGGCCCTCAC GGTTTTGGGG CGACCTCCGG GGCTCGCTCA GATCCCGTTG 
CCTGAGGCAG AGCCTGATGT GTTCGGCGTT AGGGTATCCT CCCCGAACTC TCCAGCCACC 
GAACGCTCAT GCAGTCCTCA GGACCGCCTC TTTTCCCCAC AACAACAATA TGACGAGAAC 
AACAGTTTCT GCGACCAGAG GACAAATGTC TTTGCAAAGT TTCTATCAAA GGAGCAGCAG 
GAGCTGCAGG TCATGAAAAA GGAGTACAGC AGGAACCCCT CCCCCAAACT ACTGAAAGAC 
ATTCAGGAAG CTACAAAACA CATTCCACAG CTGCAGGGTC AGCTCTTCAA GGCCACTGGG 
ACAACACAGG AGGCTGTTTC CGGTGGTGAT GAAGACAATG GTAGCATATC AGAGACAGAT 
TGGACCCCTC CTCATAAAGG AAACAACTGC ATAGACCTGC TGTGGAACAA CACTCCAAAA 
TCCCGTGTAT TCGAACCTGG ATCTCCTTCT ACCCAGAGTC CAAGAGACTC CTGCGCGAGC 
CCCAAAAGCT CTCCTAAAGA CAGCTTCAAC CCCGTCTACT CCCCAGAGAC GGAGGACGTC 
ACTGACCTGG ACACCGTCTC TCCCACCACG GTCAGCAGTC CTCTTCCCTG CCGCCTTGGC 
TTGCAAATTA TAGGAGCAGA AGACGACTAT TTTGATTCGG ACCAGGAGCA GACAAATGGC 
CAGTGTAACA GCTTTAACAG TATTGAGCAG CTCAAGTCTC GTCCTGCCCA CCTCGCAGCT 
TTTTTGCATC ATGTCATTTC TCAGTTTGAC CCTGCTCCTG TGTTGTGCTA CCTCTATGCT 
GACTATTACA AGCAAACCAA CTCCAAAGAA ACAAGAAAGG AGCTCCATAA CGTCTTCATA 
GACCGGGCAG CAATTTTAAA AGTTCCCATT CCTGAATCAG TTTCTTCTGA CCTTAACTGG 
CGACGAATGG AACTGAACCC TGAAGAAACA AACAAACAGT ATGTTAAAAC ACTGCAGGAC 
TCTCTCCTCT CTGACATTCA GAAAAACCTC GAGGACTTCA GGCAGAAACG CAGTATGGGC 
CTAACAGTGG CTAAGGCTGA GCTGACAAGA CTGGACCAAG AAAGAGTCAG AGACCGGGTC 
ACCATAGAGC GGGAGCGATC ATACGCAGAA AGCATCATCA GTAAAATAGA TGACATCCTG 
TTCACTACTC AAACCACAGA GGAAGACAAA TGCACTACGA TACAGAATGT TATCTACACA 
TACATGAAGC ACCTTGGGGT GAGAGTCAAG GAACCTCGAA TTCTGGAGGC CAAACGGGGT 
CGGAAAAGCT TTTTACCTAA AATGATGCAG AAAGGCATAA AAACTGAGAA GGAAGGAGAA 
GACAAAGGCA GAAAAGTATT TATGTTGCCC CTGCGTCGGC CCAGCCGCAT TGACTCGTCG 
CCAGCCATTA AAGCCCTGGA CAGTCGTCCC AAGCCTCAGA AACAGCTGTC TCAGCCGACG 
CCTGTGACCA GCGAGCTAAT GGAAGTTGGT CCATTGAGAG GCAGCCAATC CAGCGATTGC 
AATGACCTCA ATCACTCCAT GTCTGTCACC ACCTCATCTC CAAACATCAA CACCCACTGC 
ACAGATGTGA GCCGAGACGT CGACATCGGT GGGACGTCAT CTGGGTCCTC CAGACTGAAC 
GACGGCCTTC AGACCGACCT GGACGTGTTC CCATTCCCGT CTTCACCACT GGATGTTTGT 
GATCAGCTCC AGGAGGACGA CAGTGACCGA GCCCATGAGG GAACTCCAAA GGCCACAAGA 
AGATTTGAAG GATTGATTGC AGCTGATGTC CAAAGTGAGG ACGATCAAGG GACAGACTCT 
GAGCACAATC CTCCAAACTG GCATCAGCGG GTGGGTCGAG AAGTCCTCGT AGGCCTCAGT 
CCTCATGAAA TCAAGCGTCA GGAAGTTATC AACGAGCTGT TTTACACTGA GCGCGCTCAT 
GTGCGGATGC TGAAAGTTTT GGACCTCGTC TTCTACCAAA AGCTTTCCAA AGATGCAATT 
CTTCCTCCTA CTGACATCAA AAATATCTTC ACCAACTTGG AGGAGGTTCT GCAGCTGCAT 
GTTTCAATAC TGGAGCAAAT GACAGCTGTC CGGAAGAGAA ACGAGTCTTC CGTCATTGAT 
CAAATAGGAG ATGACTTGCT CTCTTGGTTC AGCGGCGGCG AGGAGGAGAA GATCAAGCAG 
GCAGTGGGGA CGTTTTGCAG CAATCAGCCT TTTGCTCTAG AGATTATTAA AACCAAGCAG 
AAGAAAGACT CGAGGTTCAT TTCATTCATC CAGGAGGCTG AGAGTAACAG ACTGTGTCGC 
CGGCTGCAGC TCAAAGACCT CATTCCTGTA GAGATGCTCC GGCTCACCAA GTACCCGCTG 
CTACTGGACA ACATCGTCAA GTACACAGAC ATTGCTGTGG AAAAGCAGAA AGTCAAGCAG 
GCAGCAGAAT GCTGTAGAAG GATCCTCAGT CATGTCAACC AGGCTGTGAA GGAGTCTGAG 
GACAAACAGA AATTAGAAGA CTATCAGCGA AGATTGGACC TCTCCACTCT AAAGCAGACT 
GACAACCCTC TGATCGCTGA ACTAAAGAAT CTGGATCTAA CAAAGAGGAC GATGATTCAT 
GAGGGTTCAC TGTCGTGGAA AGTGAACAGG GACAAGACTA TTGAGTTGTT CACACTCCTC 
CTTGAGGACA TCCTCGTTCT GCTGCAGAAA CAGGATGAGC GTCTGATCCT TAAGTGTCAC 
AGCAAGAACC TGGCAGGTGC TGCAGATACC AAACACATCT ACAGCCCCAT CATCAAACTC 
AGCACTGTCC TCGTGCGCTC TGTTGCTACG GACAACAGGT CCTTTTTCGT CCTCTCTGTG 
TCTGAAAACG GTTCCCACAT CTACGAGCTA ATGGCGCCCA CTGTGACGGA TCAGAACACG 
TGGCAAAGTC TGATCTCCCA GAGAGCCGAT GCAATGAAAG TCAAGCCTCA CAGCATCATC 
CCGTTACCTC AGCCTGACGG GGAGAGAGAT GGAGCGGAGA TCATAACAGC TGGTACTTCC 
CGACTGGGTA AAGACCCGGA CCGCACATCC ACCAGCAGCA CTCAGTCCAC AGATAAAGAG 
AGAGGTCCAG CCTCTAGAGG TGCATTACAG ACTTCTCTAC CTGGTAGTAA TCCTTTTGAA 
GAAGTGAAGG CTGAGGAGGA GGAGGAGGAG GGTGGCTTTG TGGACCGAGT GCTTCCTTAC 
CAAGTAGCTG AAATCGACAG AGAAGGAGAC TCGTTTGATG TGTTTCCCTC TCGAGCAGAT 
GAAGCACTGA AAACATTGGC AGCATTAAAG CACGTGTTGG TGACCCAGCT GATGTCCCAG 
GAGGCAGCAG AACAAAGCAA ACGCTCCTCT GGAGTCCGTC TCCTCAGGAC TACGTCCCTG 
CGGACCCCGA CGGACAACCG CAGCAAGGTT CTGGTCCACA ACGGCTCCGA GAAAAGTAGC 
TCCAAAACAG AAGACCTGGC TCAGGACCTG GGGTCGTGGG ACACTGGATT CTTTGAGTCA 
CCTGAAGATT ACGAAGGTTA CTGCGGCCCA CGGGAGAGCA GCACAGATGA CGACATCCTG 
GCCTCAACCA CAGGGAAGCA GCTGAACACC TCACAAGTCT GCGCTGCCGA CTCCGGCATC 
AATTTACGAT TTTCCTCCAC GTCACAGGTC GGCAGCCTCT CCACTTTCAG CAGGCAGGTG 
CTGTCGCACC TTAGAAATCT TCAGACCAAC CTCAACTATC TAAAGGAGGT GGAGGCGAAG 
TACAACAATC TAATACACCA AAGGCCAGAG AGGTCGACAA CAGACAAGGA TAAAGATAAG 
AGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015807046.1
CDS	1384..5649
Translation	MSDTQPSFTDRFNKKAIRTSSILSKDHPPDKKPKSDKTSPPSNEFDPTDALVVQKSGNNSVLAEGKPESCGLVQRCVIIQKDENGFGFTVSGDNPVFVQLVKEDGAAMRAGVQTGDRIIKVNGTLVTQSNHTEVVKLIKSGSYVALTVLGRPPGLAQIPLPEAEPDVFGVRVSSPNSPATERSCSPQDRLFSPQQQYDENNSFCDQRTNVFAKFLSKEQQELQVMKKEYSRNPSPKLLKDIQEATKHIPQLQGQLFKATGTTQEAVSGGDEDNGSISETDWTPPHKGNNCIDLLWNNTPKSRVFEPGSPSTQSPRDSCASPKSSPKDSFNPVYSPETEDVTDLDTVSPTTVSSPLPCRLGLQIIGAEDDYFDSDQEQTNGQCNSFNSIEQLKSRPAHLAAFLHHVISQFDPAPVLCYLYADYYKQTNSKETRKELHNVFIDRAAILKVPIPESVSSDLNWRRMELNPEETNKQYVKTLQDSLLSDIQKNLEDFRQKRSMGLTVAKAELTRLDQERVRDRVTIERERSYAESIISKIDDILFTTQTTEEDKCTTIQNVIYTYMKHLGVRVKEPRILEAKRGRKSFLPKMMQKGIKTEKEGEDKGRKVFMLPLRRPSRIDSSPAIKALDSRPKPQKQLSQPTPVTSELMEVGPLRGSQSSDCNDLNHSMSVTTSSPNINTHCTDVSRDVDIGGTSSGSSRLNDGLQTDLDVFPFPSSPLDVCDQLQEDDSDRAHEGTPKATRRFEGLIAADVQSEDDQGTDSEHNPPNWHQRVGREVLVGLSPHEIKRQEVINELFYTERAHVRMLKVLDLVFYQKLSKDAILPPTDIKNIFTNLEEVLQLHVSILEQMTAVRKRNESSVIDQIGDDLLSWFSGGEEEKIKQAVGTFCSNQPFALEIIKTKQKKDSRFISFIQEAESNRLCRRLQLKDLIPVEMLRLTKYPLLLDNIVKYTDIAVEKQKVKQAAECCRRILSHVNQAVKESEDKQKLEDYQRRLDLSTLKQTDNPLIAELKNLDLTKRTMIHEGSLSWKVNRDKTIELFTLLLEDILVLLQKQDERLILKCHSKNLAGAADTKHIYSPIIKLSTVLVRSVATDNRSFFVLSVSENGSHIYELMAPTVTDQNTWQSLISQRADAMKVKPHSIIPLPQPDGERDGAEIITAGTSRLGKDPDRTSTSSTQSTDKERGPASRGALQTSLPGSNPFEEVKAEEEEEEGGFVDRVLPYQVAEIDREGDSFDVFPSRADEALKTLAALKHVLVTQLMSQEAAEQSKRSSGVRLLRTTSLRTPTDNRSKVLVHNGSEKSSSKTEDLAQDLGSWDTGFFESPEDYEGYCGPRESSTDDDILASTTGKQLNTSQVCAADSGINLRFSSTSQVGSLSTFSRQVLSHLRNLQTNLNYLKEVEAKYNNLIHQRPERSTTDKDKDKR

Target ORF information:

RefSeq Version	XM_015951560.1
Organism	Nothobranchius furzeri(turquoise killifish)
Definition	Nothobranchius furzeri Rho guanine nucleotide exchange factor 12 (arhgef12), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015951560.1

ORF Insert Sequence:

ATGAGTGACA CACAGCCCAG CTTCACTGAC AGGTTTAACA AGAAAGCAAT CAGAACCAGC 
AGCATTCTCA GTAAAGACCA CCCCCCAGAC AAGAAACCCA AAAGTGACAA AACCTCACCT 
CCCTCCAATG AGTTTGACCC TACAGACGCT CTGGTGGTGC AGAAGAGTGG CAACAACAGT 
GTGCTAGCAG AGGGGAAGCC TGAGTCCTGT GGTCTAGTCC AGCGATGTGT GATCATCCAG 
AAAGATGAAA ATGGCTTTGG GTTCACTGTC AGTGGCGATA ACCCCGTGTT TGTGCAGCTG 
GTCAAAGAAG ATGGGGCTGC TATGCGGGCA GGTGTTCAGA CAGGAGACAG GATCATCAAG 
GTTAACGGGA CCCTTGTGAC ACAGTCCAAT CACACTGAAG TTGTGAAACT TATCAAATCG 
GGCTCGTATG TGGCCCTCAC GGTTTTGGGG CGACCTCCGG GGCTCGCTCA GATCCCGTTG 
CCTGAGGCAG AGCCTGATGT GTTCGGCGTT AGGGTATCCT CCCCGAACTC TCCAGCCACC 
GAACGCTCAT GCAGTCCTCA GGACCGCCTC TTTTCCCCAC AACAACAATA TGACGAGAAC 
AACAGTTTCT GCGACCAGAG GACAAATGTC TTTGCAAAGT TTCTATCAAA GGAGCAGCAG 
GAGCTGCAGG TCATGAAAAA GGAGTACAGC AGGAACCCCT CCCCCAAACT ACTGAAAGAC 
ATTCAGGAAG CTACAAAACA CATTCCACAG CTGCAGGGTC AGCTCTTCAA GGCCACTGGG 
ACAACACAGG AGGCTGTTTC CGGTGGTGAT GAAGACAATG GTAGCATATC AGAGACAGAT 
TGGACCCCTC CTCATAAAGG AAACAACTGC ATAGACCTGC TGTGGAACAA CACTCCAAAA 
TCCCGTGTAT TCGAACCTGG ATCTCCTTCT ACCCAGAGTC CAAGAGACTC CTGCGCGAGC 
CCCAAAAGCT CTCCTAAAGA CAGCTTCAAC CCCGTCTACT CCCCAGAGAC GGAGGACGTC 
ACTGACCTGG ACACCGTCTC TCCCACCACG GTCAGCAGTC CTCTTCCCTG CCGCCTTGGC 
TTGCAAATTA TAGGAGCAGA AGACGACTAT TTTGATTCGG ACCAGGAGCA GACAAATGGC 
CAGTGTAACA GCTTTAACAG TATTGAGCAG CTCAAGTCTC GTCCTGCCCA CCTCGCAGCT 
TTTTTGCATC ATGTCATTTC TCAGTTTGAC CCTGCTCCTG TGTTGTGCTA CCTCTATGCT 
GACTATTACA AGCAAACCAA CTCCAAAGAA ACAAGAAAGG AGCTCCATAA CGTCTTCATA 
GACCGGGCAG CAATTTTAAA AGTTCCCATT CCTGAATCAG TTTCTTCTGA CCTTAACTGG 
CGACGAATGG AACTGAACCC TGAAGAAACA AACAAACAGT ATGTTAAAAC ACTGCAGGAC 
TCTCTCCTCT CTGACATTCA GAAAAACCTC GAGGACTTCA GGCAGAAACG CAGTATGGGC 
CTAACAGTGG CTAAGGCTGA GCTGACAAGA CTGGACCAAG AAAGAGTCAG AGACCGGGTC 
ACCATAGAGC GGGAGCGATC ATACGCAGAA AGCATCATCA GTAAAATAGA TGACATCCTG 
TTCACTACTC AAACCACAGA GGAAGACAAA TGCACTACGA TACAGAATGT TATCTACACA 
TACATGAAGC ACCTTGGGGT GAGAGTCAAG GAACCTCGAA TTCTGGAGGC CAAACGGGGT 
CGGAAAAGCT TTTTACCTAA AATGATGCAG AAAGGCATAA AAACTGAGAA GGAAGGAGAA 
GACAAAGGCA GAAAAGTATT TATGTTGCCC CTGCGTCGGC CCAGCCGCAT TGACTCGTCG 
CCAGCCATTA AAGCCCTGGA CAGTCGTCCC AAGCCTCAGA AACAGCTGTC TCAGCCGACG 
CCTGTGACCA GCGAGCTAAT GGAAGTTGGT CCATTGAGAG GCAGCCAATC CAGCGATTGC 
AATGACCTCA ATCACTCCAT GTCTGTCACC ACCTCATCTC CAAACATCAA CACCCACTGC 
ACAGATGTGA GCCGAGACGT CGACATCGGT GGGACGTCAT CTGGGTCCTC CAGACTGAAC 
GACGGCCTTC AGACCGACCT GGACGTGTTC CCATTCCCGT CTTCACCACT GGATGTTTGT 
GATCAGCTCC AGGAGGACGA CAGTGACCGA GCCCATGAGG GAACTCCAAA GGCCACAAGA 
AGATTTGAAG GATTGATTGC AGCTGATGTC CAAAGTGAGG ACGATCAAGG GACAGACTCT 
GAGCACAATC CTCCAAACTG GCATCAGCGG GTGGGTCGAG AAGTCCTCGT AGGCCTCAGT 
CCTCATGAAA TCAAGCGTCA GGAAGTTATC AACGAGCTGT TTTACACTGA GCGCGCTCAT 
GTGCGGATGC TGAAAGTTTT GGACCTCGTC TTCTACCAAA AGCTTTCCAA AGATGCAATT 
CTTCCTCCTA CTGACATCAA AAATATCTTC ACCAACTTGG AGGAGGTTCT GCAGCTGCAT 
GTTTCAATAC TGGAGCAAAT GACAGCTGTC CGGAAGAGAA ACGAGTCTTC CGTCATTGAT 
CAAATAGGAG ATGACTTGCT CTCTTGGTTC AGCGGCGGCG AGGAGGAGAA GATCAAGCAG 
GCAGTGGGGA CGTTTTGCAG CAATCAGCCT TTTGCTCTAG AGATTATTAA AACCAAGCAG 
AAGAAAGACT CGAGGTTCAT TTCATTCATC CAGGAGGCTG AGAGTAACAG ACTGTGTCGC 
CGGCTGCAGC TCAAAGACCT CATTCCTGTA GAGATGCTCC GGCTCACCAA GTACCCGCTG 
CTACTGGACA ACATCGTCAA GTACACAGAC ATTGCTGTGG AAAAGCAGAA AGTCAAGCAG 
GCAGCAGAAT GCTGTAGAAG GATCCTCAGT CATGTCAACC AGGCTGTGAA GGAGTCTGAG 
GACAAACAGA AATTAGAAGA CTATCAGCGA AGATTGGACC TCTCCACTCT AAAGCAGACT 
GACAACCCTC TGATCGCTGA ACTAAAGAAT CTGGATCTAA CAAAGAGGAC GATGATTCAT 
GAGGGTTCAC TGTCGTGGAA AGTGAACAGG GACAAGACTA TTGAGTTGTT CACACTCCTC 
CTTGAGGACA TCCTCGTTCT GCTGCAGAAA CAGGATGAGC GTCTGATCCT TAAGTGTCAC 
AGCAAGAACC TGGCAGGTGC TGCAGATACC AAACACATCT ACAGCCCCAT CATCAAACTC 
AGCACTGTCC TCGTGCGCTC TGTTGCTACG GACAACAGGT CCTTTTTCGT CCTCTCTGTG 
TCTGAAAACG GTTCCCACAT CTACGAGCTA ATGGCGCCCA CTGTGACGGA TCAGAACACG 
TGGCAAAGTC TGATCTCCCA GAGAGCCGAT GCAATGAAAG TCAAGCCTCA CAGCATCATC 
CCGTTACCTC AGCCTGACGG GGAGAGAGAT GGAGCGGAGA TCATAACAGC TGGTACTTCC 
CGACTGGGTA AAGACCCGGA CCGCACATCC ACCAGCAGCA CTCAGTCCAC AGATAAAGAG 
AGAGGTCCAG CCTCTAGAGG TGCATTACAG ACTTCTCTAC CTGGTAGTAA TCCTTTTGAA 
GAAGTGAAGG CTGAGGAGGA GGAGGAGGAG GGTGGCTTTG TGGACCGAGT GCTTCCTTAC 
CAAGTAGCTG AAATCGACAG AGAAGGAGAC TCGTTTGATG TGTTTCCCTC TCGAGCAGAT 
GAAGCACTGA AAACATTGGC AGCATTAAAG CACGTGTTGG TGACCCAGCT GATGTCCCAG 
GAGGCAGCAG AACAAAGCAA ACGCTCCTCT GGAGTCCGTC TCCTCAGGAC TACGTCCCTG 
CGGACCCCGA CGGACAACCG CAGCAAGGTT CTGGTCCACA ACGGCTCCGA GAAAAGTAGC 
TCCAAAACAG AAGACCTGGC TCAGGACCTG GGGTCGTGGG ACACTGGATT CTTTGAGTCA 
CCTGAAGATT ACGAAGGTTA CTGCGGCCCA CGGGAGAGCA GCACAGATGA CGACATCCTG 
GCCTCAACCA CAGGGAAGCA GCTGAACACC TCACAAGTCT GCGCTGCCGA CTCCGGCATC 
AATTTACGAT TTTCCTCCAC GTCACAGGTC GGCAGCCTCT CCACTTTCAG CAGGCAGGTG 
CTGTCGCACC TTAGAAATCT TCAGACCAAC CTCAACTATC TAAAGGAGGT GGAGGCGAAG 
TACAACAATC TAATACACCA AAGGCCAGAG AGGTCGACAA CAGACAAGGA TAAAGATAAG 
AGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

arhgef12 ( XM_015951561.1 ) cDNA ORF clone, Nothobranchius furzeri(turquoise killifish) -> XP_015807047.1 Nothobranchius furzeri Rho guanine nucleotide exchange factor 12 (arhgef12), transcript variant X4, mRNA.

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CloneID	ONo10373
Clone ID Related Accession (Same CDS sequence)	XM_015951561.1
Accession Version	XM_015951561.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4263bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-03-17
Organism	Nothobranchius furzeri(turquoise killifish)
Product	rho guanine nucleotide exchange factor 12 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015443528.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Nothobranchius furzeri Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGACA CACAGCCCAG CTTCACTGAC AGGTTTAACA AGAAAGCAAT CAGAACCAGC 
AGCATTCTCA GTAAAGACCA CCCCCCAGAC AAGAAACCCA AAAGTGACAA AACCTCACCT 
CCCTCCAATG AGTTTGACCC TACAGACGCT CTGGTGGTGC AGAAGAGTGG CAACAACAGT 
GTGCTAGCAG AGGGGAAGCC TGAGTCCTGT GGTCTAGTCC AGCGATGTGT GATCATCCAG 
AAAGATGAAA ATGGCTTTGG GTTCACTGTC AGTGGCGATA ACCCCGTGTT TGTGCAGCTG 
GTCAAAGAAG ATGGGGCTGC TATGCGGGCA GGTGTTCAGA CAGGAGACAG GATCATCAAG 
GTTAACGGGA CCCTTGTGAC ACAGTCCAAT CACACTGAAG TTGTGAAACT TATCAAATCG 
GGCTCGTATG TGGCCCTCAC GGTTTTGGGG CGACCTCCGG GGCTCGCTCA GATCCCGTTG 
CCTGAGGCAG AGCCTGATGT GTTCGGCGTT AGGGTATCCT CCCCGAACTC TCCAGCCACC 
GAACGCTCAT GCAGTCCTCA GGACCGCCTC TTTTCCCCAC AACAACAATA TGACGAGAAC 
AACAGTTTCT GCGACCAGAG GACAAATGTC TTTGCAAAGT TTCTATCAAA GGAGCAGCAG 
GAGCTGCAGG TCATGAAAAA GGAGTACAGC AGGAACCCCT CCCCCAAACT ACTGAAAGAC 
ATTCAGGAAG CTACAAAACA CATTCCACAG CTGCAGGGTC AGCTCTTCAA GGCCACTGGG 
ACAACACAGG AGGCTGTTTC CGGTGGTGAT GAAGACAATG GTAGCATATC AGAGACAGAT 
TGGACCCCTC CTCATAAAGG AAACAACTGC ATAGACCTGC TGTGGAACAA CACTCCAAAA 
TCCCGTGTAT TCGAACCTGG ATCTCCTTCT ACCCAGAGTC CAAGAGACTC CTGCGCGAGC 
CCCAAAAGCT CTCCTAAAGA CAGCTTCAAC CCCGTCTACT CCCCAGAGAC GGAGGACGTC 
ACTGACCTGG ACACCGTCTC TCCCACCACG GTCAGCAGTC CTCTTCCCTG CCGCCTTGGC 
TTGCAAATTA TAGGAGCAGA AGACGACTAT TTTGATTCGG ACCAGGAGCA GACAAATGGC 
CAGTGTAACA GCTTTAACAG TATTGAGCAG CTCAAGTCTC GTCCTGCCCA CCTCGCAGCT 
TTTTTGCATC ATGTCATTTC TCAGTTTGAC CCTGCTCCTG TGTTGTGCTA CCTCTATGCT 
GACTATTACA AGCAAACCAA CTCCAAAGAA ACAAGAAAGG AGCTCCATAA CGTCTTCATA 
GACCGGGCAG CAATTTTAAA AGTTCCCATT CCTGAATCAG TTTCTTCTGA CCTTAACTGG 
CGACGAATGG AACTGAACCC TGAAGAAACA AACAAACAGT ATGTTAAAAC ACTGCAGGAC 
TCTCTCCTCT CTGACATTCA GAAAAACCTC GAGGACTTCA GGCAGAAACG CAGTATGGGC 
CTAACAGTGG CTAAGGCTGA GCTGACAAGA CTGGACCAAG AAAGAGTCAG AGACCGGGTC 
ACCATAGAGC GGGAGCGATC ATACGCAGAA AGCATCATCA GTAAAATAGA TGACATCCTG 
TTCACTACTC AAACCACAGA GGAAGACAAA TGCACTACGA TACAGAATGT TATCTACACA 
TACATGAAGC ACCTTGGGGT GAGAGTCAAG GAACCTCGAA TTCTGGAGGC CAAACGGGGT 
CGGAAAAGCT TTTTACCTAA AATGATGCAG AAAGGCATAA AAACTGAGAA GGAAGGAGAA 
GACAAAGGCA GAAAAGTATT TATGTTGCCC CTGCGTCGGC CCAGCCGCAT TGACTCGTCG 
CCAGCCATTA AAGCCCTGGA CAGTCGTCCC AAGCCTCAGA AACAGCTGTC TCAGCCGACG 
CCTGTGACCA GCGAGCTAAT GGAAGTTGGT CCATTGAGAG GCAGCCAATC CAGCGATTGC 
AATGACCTCA ATCACTCCAT GTCTGTCACC ACCTCATCTC CAAACATCAA CACCCACTGC 
ACAGATGTGA GCCGAGACGT CGACATCGGT GGGACGTCAT CTGGGTCCTC CAGACTGAAC 
GACGGCCTTC AGACCGACCT GGACGTGTTC CCATTCCCGT CTTCACCACT GGATGTTTGT 
GATCAGCTCC AGGAGGACGA CAGTGACCGA GCCCATGAGG GAACTCCAAA GGCCACAAGA 
AGATTTGAAG GATTGATTGC AGCTGATGTC CAAAGTGAGG ACGATCAAGG GACAGACTCT 
GAGCACAATC CTCCAAACTG GCATCAGCGG GTGGGTCGAG AAGTCCTCGT AGGCCTCAGT 
CCTCATGAAA TCAAGCGTCA GGAAGTTATC AACGAGCTGT TTTACACTGA GCGCGCTCAT 
GTGCGGATGC TGAAAGTTTT GGACCTCGTC TTCTACCAAA AGCTTTCCAA AGATGCAATT 
CTTCCTCCTA CTGACATCAA AAATATCTTC ACCAACTTGG AGGAGGTTCT GCAGCTGCAT 
GTTTCAATAC TGGAGCAAAT GACAGCTGTC CGGAAGAGAA ACGAGTCTTC CGTCATTGAT 
CAAATAGGAG ATGACTTGCT CTCTTGGTTC AGCGGCGGCG AGGAGGAGAA GATCAAGCAG 
GCAGTGGGGA CGTTTTGCAG CAATCAGCCT TTTGCTCTAG AGATTATTAA AACCAAGCAG 
AAGAAAGACT CGAGGTTCAT TTCATTCATC CAGGAGGCTG AGAGTAACAG ACTGTGTCGC 
CGGCTGCAGC TCAAAGACCT CATTCCTGTA GAGATGCTCC GGCTCACCAA GTACCCGCTG 
CTACTGGACA ACATCGTCAA GTACACAGAC ATTGCTGTGG AAAAGCAGAA AGTCAAGCAG 
GCAGCAGAAT GCTGTAGAAG GATCCTCAGT CATGTCAACC AGGCTGTGAA GGAGTCTGAG 
GACAAACAGA AATTAGAAGA CTATCAGCGA AGATTGGACC TCTCCACTCT AAAGCAGACT 
GACAACCCTC TGATCGCTGA ACTAAAGAAT CTGGATCTAA CAAAGAGGAC GATGATTCAT 
GAGGGTTCAC TGTCGTGGAA AGTGAACAGG GACAAGACTA TTGAGTTGTT CACACTCCTC 
CTTGAGGACA TCCTCGTTCT GCTGCAGAAA CAGGATGAGC GTCTGATCCT TAAGTGTCAC 
AGCAAGAACC TGGCAGGTGC TGCAGATACC AAACACATCT ACAGCCCCAT CATCAAACTC 
AGCACTGTCC TCGTGCGCTC TGTTGCTACG GACAACAGGT CCTTTTTCGT CCTCTCTGTG 
TCTGAAAACG GTTCCCACAT CTACGAGCTA ATGGCGCCCA CTGTGACGGA TCAGAACACG 
TGGCAAAGTC TGATCTCCCA GAGAGCCGAT GCAATGAAAG TCAAGCCTCA CAGCATCATC 
CCGTTACCTC AGCCTGACGG GGAGAGAGAT GGAGCGGAGA TCATAACAGC TGGTACTTCC 
CGACTGGGTA AAGACCCGGA CCGCACATCC ACCAGCAGCA CTCAGTCCAC AGATAAAGAG 
AGAGGTCCAG CCTCTAGAGG TGCATTACAG ACTTCTCTAC CTGGTAGTAA TCCTTTTGAA 
GAAGTGAAGG CTGAGGAGGA GGAGGAGGAG GGTGGCTTTG TGGACCGAGT GCTTCCTTAC 
CAAGTAGCTG AAATCGACAG AGAAGGAGAC TCGTTTGATG TGTTTCCCTC TCGAGCAGAT 
GAAGCACTGA AAACATTGGC AGCATTAAAG CACGTGTTGG TGACCCAGCT GATGTCCCAG 
GAGGCAGCAG AACAAAGCAA ACGCTCCTCT GGAGTCCGTC TCCTCAGGAC TACGTCCCTG 
CGGACCCCGA CGGACAACCG CAGCAAGGTT CTGGTCCACA ACGGCTCCGA GAAAAGTAGC 
TCCAAAACAG AAGACCTGGC TCAGGACCTG GGGTCGTGGG ACACTGGATT CTTTGAGTCA 
CCTGAAGATT ACGGTTACTG CGGCCCACGG GAGAGCAGCA CAGATGACGA CATCCTGGCC 
TCAACCACAG GGAAGCAGCT GAACACCTCA CAAGTCTGCG CTGCCGACTC CGGCATCAAT 
TTACGATTTT CCTCCACGTC ACAGGTCGGC AGCCTCTCCA CTTTCAGCAG GCAGGTGCTG 
TCGCACCTTA GAAATCTTCA GACCAACCTC AACTATCTAA AGGAGGTGGA GGCGAAGTAC 
AACAATCTAA TACACCAAAG GCCAGAGAGG TCGACAACAG ACAAGGATAA AGATAAGAGA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015807047.1
CDS	1385..5647
Translation	MSDTQPSFTDRFNKKAIRTSSILSKDHPPDKKPKSDKTSPPSNEFDPTDALVVQKSGNNSVLAEGKPESCGLVQRCVIIQKDENGFGFTVSGDNPVFVQLVKEDGAAMRAGVQTGDRIIKVNGTLVTQSNHTEVVKLIKSGSYVALTVLGRPPGLAQIPLPEAEPDVFGVRVSSPNSPATERSCSPQDRLFSPQQQYDENNSFCDQRTNVFAKFLSKEQQELQVMKKEYSRNPSPKLLKDIQEATKHIPQLQGQLFKATGTTQEAVSGGDEDNGSISETDWTPPHKGNNCIDLLWNNTPKSRVFEPGSPSTQSPRDSCASPKSSPKDSFNPVYSPETEDVTDLDTVSPTTVSSPLPCRLGLQIIGAEDDYFDSDQEQTNGQCNSFNSIEQLKSRPAHLAAFLHHVISQFDPAPVLCYLYADYYKQTNSKETRKELHNVFIDRAAILKVPIPESVSSDLNWRRMELNPEETNKQYVKTLQDSLLSDIQKNLEDFRQKRSMGLTVAKAELTRLDQERVRDRVTIERERSYAESIISKIDDILFTTQTTEEDKCTTIQNVIYTYMKHLGVRVKEPRILEAKRGRKSFLPKMMQKGIKTEKEGEDKGRKVFMLPLRRPSRIDSSPAIKALDSRPKPQKQLSQPTPVTSELMEVGPLRGSQSSDCNDLNHSMSVTTSSPNINTHCTDVSRDVDIGGTSSGSSRLNDGLQTDLDVFPFPSSPLDVCDQLQEDDSDRAHEGTPKATRRFEGLIAADVQSEDDQGTDSEHNPPNWHQRVGREVLVGLSPHEIKRQEVINELFYTERAHVRMLKVLDLVFYQKLSKDAILPPTDIKNIFTNLEEVLQLHVSILEQMTAVRKRNESSVIDQIGDDLLSWFSGGEEEKIKQAVGTFCSNQPFALEIIKTKQKKDSRFISFIQEAESNRLCRRLQLKDLIPVEMLRLTKYPLLLDNIVKYTDIAVEKQKVKQAAECCRRILSHVNQAVKESEDKQKLEDYQRRLDLSTLKQTDNPLIAELKNLDLTKRTMIHEGSLSWKVNRDKTIELFTLLLEDILVLLQKQDERLILKCHSKNLAGAADTKHIYSPIIKLSTVLVRSVATDNRSFFVLSVSENGSHIYELMAPTVTDQNTWQSLISQRADAMKVKPHSIIPLPQPDGERDGAEIITAGTSRLGKDPDRTSTSSTQSTDKERGPASRGALQTSLPGSNPFEEVKAEEEEEEGGFVDRVLPYQVAEIDREGDSFDVFPSRADEALKTLAALKHVLVTQLMSQEAAEQSKRSSGVRLLRTTSLRTPTDNRSKVLVHNGSEKSSSKTEDLAQDLGSWDTGFFESPEDYGYCGPRESSTDDDILASTTGKQLNTSQVCAADSGINLRFSSTSQVGSLSTFSRQVLSHLRNLQTNLNYLKEVEAKYNNLIHQRPERSTTDKDKDKR

Target ORF information:

RefSeq Version	XM_015951561.1
Organism	Nothobranchius furzeri(turquoise killifish)
Definition	Nothobranchius furzeri Rho guanine nucleotide exchange factor 12 (arhgef12), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015951561.1

ORF Insert Sequence:

ATGAGTGACA CACAGCCCAG CTTCACTGAC AGGTTTAACA AGAAAGCAAT CAGAACCAGC 
AGCATTCTCA GTAAAGACCA CCCCCCAGAC AAGAAACCCA AAAGTGACAA AACCTCACCT 
CCCTCCAATG AGTTTGACCC TACAGACGCT CTGGTGGTGC AGAAGAGTGG CAACAACAGT 
GTGCTAGCAG AGGGGAAGCC TGAGTCCTGT GGTCTAGTCC AGCGATGTGT GATCATCCAG 
AAAGATGAAA ATGGCTTTGG GTTCACTGTC AGTGGCGATA ACCCCGTGTT TGTGCAGCTG 
GTCAAAGAAG ATGGGGCTGC TATGCGGGCA GGTGTTCAGA CAGGAGACAG GATCATCAAG 
GTTAACGGGA CCCTTGTGAC ACAGTCCAAT CACACTGAAG TTGTGAAACT TATCAAATCG 
GGCTCGTATG TGGCCCTCAC GGTTTTGGGG CGACCTCCGG GGCTCGCTCA GATCCCGTTG 
CCTGAGGCAG AGCCTGATGT GTTCGGCGTT AGGGTATCCT CCCCGAACTC TCCAGCCACC 
GAACGCTCAT GCAGTCCTCA GGACCGCCTC TTTTCCCCAC AACAACAATA TGACGAGAAC 
AACAGTTTCT GCGACCAGAG GACAAATGTC TTTGCAAAGT TTCTATCAAA GGAGCAGCAG 
GAGCTGCAGG TCATGAAAAA GGAGTACAGC AGGAACCCCT CCCCCAAACT ACTGAAAGAC 
ATTCAGGAAG CTACAAAACA CATTCCACAG CTGCAGGGTC AGCTCTTCAA GGCCACTGGG 
ACAACACAGG AGGCTGTTTC CGGTGGTGAT GAAGACAATG GTAGCATATC AGAGACAGAT 
TGGACCCCTC CTCATAAAGG AAACAACTGC ATAGACCTGC TGTGGAACAA CACTCCAAAA 
TCCCGTGTAT TCGAACCTGG ATCTCCTTCT ACCCAGAGTC CAAGAGACTC CTGCGCGAGC 
CCCAAAAGCT CTCCTAAAGA CAGCTTCAAC CCCGTCTACT CCCCAGAGAC GGAGGACGTC 
ACTGACCTGG ACACCGTCTC TCCCACCACG GTCAGCAGTC CTCTTCCCTG CCGCCTTGGC 
TTGCAAATTA TAGGAGCAGA AGACGACTAT TTTGATTCGG ACCAGGAGCA GACAAATGGC 
CAGTGTAACA GCTTTAACAG TATTGAGCAG CTCAAGTCTC GTCCTGCCCA CCTCGCAGCT 
TTTTTGCATC ATGTCATTTC TCAGTTTGAC CCTGCTCCTG TGTTGTGCTA CCTCTATGCT 
GACTATTACA AGCAAACCAA CTCCAAAGAA ACAAGAAAGG AGCTCCATAA CGTCTTCATA 
GACCGGGCAG CAATTTTAAA AGTTCCCATT CCTGAATCAG TTTCTTCTGA CCTTAACTGG 
CGACGAATGG AACTGAACCC TGAAGAAACA AACAAACAGT ATGTTAAAAC ACTGCAGGAC 
TCTCTCCTCT CTGACATTCA GAAAAACCTC GAGGACTTCA GGCAGAAACG CAGTATGGGC 
CTAACAGTGG CTAAGGCTGA GCTGACAAGA CTGGACCAAG AAAGAGTCAG AGACCGGGTC 
ACCATAGAGC GGGAGCGATC ATACGCAGAA AGCATCATCA GTAAAATAGA TGACATCCTG 
TTCACTACTC AAACCACAGA GGAAGACAAA TGCACTACGA TACAGAATGT TATCTACACA 
TACATGAAGC ACCTTGGGGT GAGAGTCAAG GAACCTCGAA TTCTGGAGGC CAAACGGGGT 
CGGAAAAGCT TTTTACCTAA AATGATGCAG AAAGGCATAA AAACTGAGAA GGAAGGAGAA 
GACAAAGGCA GAAAAGTATT TATGTTGCCC CTGCGTCGGC CCAGCCGCAT TGACTCGTCG 
CCAGCCATTA AAGCCCTGGA CAGTCGTCCC AAGCCTCAGA AACAGCTGTC TCAGCCGACG 
CCTGTGACCA GCGAGCTAAT GGAAGTTGGT CCATTGAGAG GCAGCCAATC CAGCGATTGC 
AATGACCTCA ATCACTCCAT GTCTGTCACC ACCTCATCTC CAAACATCAA CACCCACTGC 
ACAGATGTGA GCCGAGACGT CGACATCGGT GGGACGTCAT CTGGGTCCTC CAGACTGAAC 
GACGGCCTTC AGACCGACCT GGACGTGTTC CCATTCCCGT CTTCACCACT GGATGTTTGT 
GATCAGCTCC AGGAGGACGA CAGTGACCGA GCCCATGAGG GAACTCCAAA GGCCACAAGA 
AGATTTGAAG GATTGATTGC AGCTGATGTC CAAAGTGAGG ACGATCAAGG GACAGACTCT 
GAGCACAATC CTCCAAACTG GCATCAGCGG GTGGGTCGAG AAGTCCTCGT AGGCCTCAGT 
CCTCATGAAA TCAAGCGTCA GGAAGTTATC AACGAGCTGT TTTACACTGA GCGCGCTCAT 
GTGCGGATGC TGAAAGTTTT GGACCTCGTC TTCTACCAAA AGCTTTCCAA AGATGCAATT 
CTTCCTCCTA CTGACATCAA AAATATCTTC ACCAACTTGG AGGAGGTTCT GCAGCTGCAT 
GTTTCAATAC TGGAGCAAAT GACAGCTGTC CGGAAGAGAA ACGAGTCTTC CGTCATTGAT 
CAAATAGGAG ATGACTTGCT CTCTTGGTTC AGCGGCGGCG AGGAGGAGAA GATCAAGCAG 
GCAGTGGGGA CGTTTTGCAG CAATCAGCCT TTTGCTCTAG AGATTATTAA AACCAAGCAG 
AAGAAAGACT CGAGGTTCAT TTCATTCATC CAGGAGGCTG AGAGTAACAG ACTGTGTCGC 
CGGCTGCAGC TCAAAGACCT CATTCCTGTA GAGATGCTCC GGCTCACCAA GTACCCGCTG 
CTACTGGACA ACATCGTCAA GTACACAGAC ATTGCTGTGG AAAAGCAGAA AGTCAAGCAG 
GCAGCAGAAT GCTGTAGAAG GATCCTCAGT CATGTCAACC AGGCTGTGAA GGAGTCTGAG 
GACAAACAGA AATTAGAAGA CTATCAGCGA AGATTGGACC TCTCCACTCT AAAGCAGACT 
GACAACCCTC TGATCGCTGA ACTAAAGAAT CTGGATCTAA CAAAGAGGAC GATGATTCAT 
GAGGGTTCAC TGTCGTGGAA AGTGAACAGG GACAAGACTA TTGAGTTGTT CACACTCCTC 
CTTGAGGACA TCCTCGTTCT GCTGCAGAAA CAGGATGAGC GTCTGATCCT TAAGTGTCAC 
AGCAAGAACC TGGCAGGTGC TGCAGATACC AAACACATCT ACAGCCCCAT CATCAAACTC 
AGCACTGTCC TCGTGCGCTC TGTTGCTACG GACAACAGGT CCTTTTTCGT CCTCTCTGTG 
TCTGAAAACG GTTCCCACAT CTACGAGCTA ATGGCGCCCA CTGTGACGGA TCAGAACACG 
TGGCAAAGTC TGATCTCCCA GAGAGCCGAT GCAATGAAAG TCAAGCCTCA CAGCATCATC 
CCGTTACCTC AGCCTGACGG GGAGAGAGAT GGAGCGGAGA TCATAACAGC TGGTACTTCC 
CGACTGGGTA AAGACCCGGA CCGCACATCC ACCAGCAGCA CTCAGTCCAC AGATAAAGAG 
AGAGGTCCAG CCTCTAGAGG TGCATTACAG ACTTCTCTAC CTGGTAGTAA TCCTTTTGAA 
GAAGTGAAGG CTGAGGAGGA GGAGGAGGAG GGTGGCTTTG TGGACCGAGT GCTTCCTTAC 
CAAGTAGCTG AAATCGACAG AGAAGGAGAC TCGTTTGATG TGTTTCCCTC TCGAGCAGAT 
GAAGCACTGA AAACATTGGC AGCATTAAAG CACGTGTTGG TGACCCAGCT GATGTCCCAG 
GAGGCAGCAG AACAAAGCAA ACGCTCCTCT GGAGTCCGTC TCCTCAGGAC TACGTCCCTG 
CGGACCCCGA CGGACAACCG CAGCAAGGTT CTGGTCCACA ACGGCTCCGA GAAAAGTAGC 
TCCAAAACAG AAGACCTGGC TCAGGACCTG GGGTCGTGGG ACACTGGATT CTTTGAGTCA 
CCTGAAGATT ACGGTTACTG CGGCCCACGG GAGAGCAGCA CAGATGACGA CATCCTGGCC 
TCAACCACAG GGAAGCAGCT GAACACCTCA CAAGTCTGCG CTGCCGACTC CGGCATCAAT 
TTACGATTTT CCTCCACGTC ACAGGTCGGC AGCCTCTCCA CTTTCAGCAG GCAGGTGCTG 
TCGCACCTTA GAAATCTTCA GACCAACCTC AACTATCTAA AGGAGGTGGA GGCGAAGTAC 
AACAATCTAA TACACCAAAG GCCAGAGAGG TCGACAACAG ACAAGGATAA AGATAAGAGA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

arhgef12 ( XM_015951562.1 ) cDNA ORF clone, Nothobranchius furzeri(turquoise killifish) -> XP_015807048.1 Nothobranchius furzeri Rho guanine nucleotide exchange factor 12 (arhgef12), transcript variant X5, mRNA.

Price & Availability↑

CloneID	ONo10374
Clone ID Related Accession (Same CDS sequence)	XM_015951562.1
Accession Version	XM_015951562.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4215bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-03-17
Organism	Nothobranchius furzeri(turquoise killifish)
Product	rho guanine nucleotide exchange factor 12 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015443528.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Nothobranchius furzeri Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGACA CACAGCCCAG CTTCACTGAC AGGTTTAACA AGAAAGCAAT CAGAACCAGC 
AGCATTCTCA GTAAAGACCA CCCCCCAGAC AAGAAACCCA AAAGTGACAA AACCTCACCT 
CCCTCCAATG AGTTTGACCC TACAGGTCTA GTCCAGCGAT GTGTGATCAT CCAGAAAGAT 
GAAAATGGCT TTGGGTTCAC TGTCAGTGGC GATAACCCCG TGTTTGTGCA GCTGGTCAAA 
GAAGATGGGG CTGCTATGCG GGCAGGTGTT CAGACAGGAG ACAGGATCAT CAAGGTTAAC 
GGGACCCTTG TGACACAGTC CAATCACACT GAAGTTGTGA AACTTATCAA ATCGGGCTCG 
TATGTGGCCC TCACGGTTTT GGGGCGACCT CCGGGGCTCG CTCAGATCCC GTTGCCTGAG 
GCAGAGCCTG ATGTGTTCGG CGTTAGGGTA TCCTCCCCGA ACTCTCCAGC CACCGAACGC 
TCATGCAGTC CTCAGGACCG CCTCTTTTCC CCACAACAAC AATATGACGA GAACAACAGT 
TTCTGCGACC AGAGGACAAA TGTCTTTGCA AAGTTTCTAT CAAAGGAGCA GCAGGAGCTG 
CAGGTCATGA AAAAGGAGTA CAGCAGGAAC CCCTCCCCCA AACTACTGAA AGACATTCAG 
GAAGCTACAA AACACATTCC ACAGCTGCAG GGTCAGCTCT TCAAGGCCAC TGGGACAACA 
CAGGAGGCTG TTTCCGGTGG TGATGAAGAC AATGGTAGCA TATCAGAGAC AGATTGGACC 
CCTCCTCATA AAGGAAACAA CTGCATAGAC CTGCTGTGGA ACAACACTCC AAAATCCCGT 
GTATTCGAAC CTGGATCTCC TTCTACCCAG AGTCCAAGAG ACTCCTGCGC GAGCCCCAAA 
AGCTCTCCTA AAGACAGCTT CAACCCCGTC TACTCCCCAG AGACGGAGGA CGTCACTGAC 
CTGGACACCG TCTCTCCCAC CACGGTCAGC AGTCCTCTTC CCTGCCGCCT TGGCTTGCAA 
ATTATAGGAG CAGAAGACGA CTATTTTGAT TCGGACCAGG AGCAGACAAA TGGCCAGTGT 
AACAGCTTTA ACAGTATTGA GCAGCTCAAG TCTCGTCCTG CCCACCTCGC AGCTTTTTTG 
CATCATGTCA TTTCTCAGTT TGACCCTGCT CCTGTGTTGT GCTACCTCTA TGCTGACTAT 
TACAAGCAAA CCAACTCCAA AGAAACAAGA AAGGAGCTCC ATAACGTCTT CATAGACCGG 
GCAGCAATTT TAAAAGTTCC CATTCCTGAA TCAGTTTCTT CTGACCTTAA CTGGCGACGA 
ATGGAACTGA ACCCTGAAGA AACAAACAAA CAGTATGTTA AAACACTGCA GGACTCTCTC 
CTCTCTGACA TTCAGAAAAA CCTCGAGGAC TTCAGGCAGA AACGCAGTAT GGGCCTAACA 
GTGGCTAAGG CTGAGCTGAC AAGACTGGAC CAAGAAAGAG TCAGAGACCG GGTCACCATA 
GAGCGGGAGC GATCATACGC AGAAAGCATC ATCAGTAAAA TAGATGACAT CCTGTTCACT 
ACTCAAACCA CAGAGGAAGA CAAATGCACT ACGATACAGA ATGTTATCTA CACATACATG 
AAGCACCTTG GGGTGAGAGT CAAGGAACCT CGAATTCTGG AGGCCAAACG GGGTCGGAAA 
AGCTTTTTAC CTAAAATGAT GCAGAAAGGC ATAAAAACTG AGAAGGAAGG AGAAGACAAA 
GGCAGAAAAG TATTTATGTT GCCCCTGCGT CGGCCCAGCC GCATTGACTC GTCGCCAGCC 
ATTAAAGCCC TGGACAGTCG TCCCAAGCCT CAGAAACAGC TGTCTCAGCC GACGCCTGTG 
ACCAGCGAGC TAATGGAAGT TGGTCCATTG AGAGGCAGCC AATCCAGCGA TTGCAATGAC 
CTCAATCACT CCATGTCTGT CACCACCTCA TCTCCAAACA TCAACACCCA CTGCACAGAT 
GTGAGCCGAG ACGTCGACAT CGGTGGGACG TCATCTGGGT CCTCCAGACT GAACGACGGC 
CTTCAGACCG ACCTGGACGT GTTCCCATTC CCGTCTTCAC CACTGGATGT TTGTGATCAG 
CTCCAGGAGG ACGACAGTGA CCGAGCCCAT GAGGGAACTC CAAAGGCCAC AAGAAGATTT 
GAAGGATTGA TTGCAGCTGA TGTCCAAAGT GAGGACGATC AAGGGACAGA CTCTGAGCAC 
AATCCTCCAA ACTGGCATCA GCGGGTGGGT CGAGAAGTCC TCGTAGGCCT CAGTCCTCAT 
GAAATCAAGC GTCAGGAAGT TATCAACGAG CTGTTTTACA CTGAGCGCGC TCATGTGCGG 
ATGCTGAAAG TTTTGGACCT CGTCTTCTAC CAAAAGCTTT CCAAAGATGC AATTCTTCCT 
CCTACTGACA TCAAAAATAT CTTCACCAAC TTGGAGGAGG TTCTGCAGCT GCATGTTTCA 
ATACTGGAGC AAATGACAGC TGTCCGGAAG AGAAACGAGT CTTCCGTCAT TGATCAAATA 
GGAGATGACT TGCTCTCTTG GTTCAGCGGC GGCGAGGAGG AGAAGATCAA GCAGGCAGTG 
GGGACGTTTT GCAGCAATCA GCCTTTTGCT CTAGAGATTA TTAAAACCAA GCAGAAGAAA 
GACTCGAGGT TCATTTCATT CATCCAGGAG GCTGAGAGTA ACAGACTGTG TCGCCGGCTG 
CAGCTCAAAG ACCTCATTCC TGTAGAGATG CTCCGGCTCA CCAAGTACCC GCTGCTACTG 
GACAACATCG TCAAGTACAC AGACATTGCT GTGGAAAAGC AGAAAGTCAA GCAGGCAGCA 
GAATGCTGTA GAAGGATCCT CAGTCATGTC AACCAGGCTG TGAAGGAGTC TGAGGACAAA 
CAGAAATTAG AAGACTATCA GCGAAGATTG GACCTCTCCA CTCTAAAGCA GACTGACAAC 
CCTCTGATCG CTGAACTAAA GAATCTGGAT CTAACAAAGA GGACGATGAT TCATGAGGGT 
TCACTGTCGT GGAAAGTGAA CAGGGACAAG ACTATTGAGT TGTTCACACT CCTCCTTGAG 
GACATCCTCG TTCTGCTGCA GAAACAGGAT GAGCGTCTGA TCCTTAAGTG TCACAGCAAG 
AACCTGGCAG GTGCTGCAGA TACCAAACAC ATCTACAGCC CCATCATCAA ACTCAGCACT 
GTCCTCGTGC GCTCTGTTGC TACGGACAAC AGGTCCTTTT TCGTCCTCTC TGTGTCTGAA 
AACGGTTCCC ACATCTACGA GCTAATGGCG CCCACTGTGA CGGATCAGAA CACGTGGCAA 
AGTCTGATCT CCCAGAGAGC CGATGCAATG AAAGTCAAGC CTCACAGCAT CATCCCGTTA 
CCTCAGCCTG ACGGGGAGAG AGATGGAGCG GAGATCATAA CAGCTGGTAC TTCCCGACTG 
GGTAAAGACC CGGACCGCAC ATCCACCAGC AGCACTCAGT CCACAGATAA AGAGAGAGGT 
CCAGCCTCTA GAGGTGCATT ACAGACTTCT CTACCTGGTA GTAATCCTTT TGAAGAAGTG 
AAGGCTGAGG AGGAGGAGGA GGAGGGTGGC TTTGTGGACC GAGTGCTTCC TTACCAAGTA 
GCTGAAATCG ACAGAGAAGG AGACTCGTTT GATGTGTTTC CCTCTCGAGC AGATGAAGCA 
CTGAAAACAT TGGCAGCATT AAAGCACGTG TTGGTGACCC AGCTGATGTC CCAGGAGGCA 
GCAGAACAAA GCAAACGCTC CTCTGGAGTC CGTCTCCTCA GGACTACGTC CCTGCGGACC 
CCGACGGACA ACCGCAGCAA GGTTCTGGTC CACAACGGCT CCGAGAAAAG TAGCTCCAAA 
ACAGAAGACC TGGCTCAGGA CCTGGGGTCG TGGGACACTG GATTCTTTGA GTCACCTGAA 
GATTACGGAT GTTTGGTCCT AGAAGGTTAC TGCGGCCCAC GGGAGAGCAG CACAGATGAC 
GACATCCTGG CCTCAACCAC AGGGAAGCAG CTGAACACCT CACAAGTCTG CGCTGCCGAC 
TCCGGCATCA ATTTACGATT TTCCTCCACG TCACAGGTCG GCAGCCTCTC CACTTTCAGC 
AGGCAGGTGC TGTCGCACCT TAGAAATCTT CAGACCAACC TCAACTATCT AAAGGAGGTG 
GAGGCGAAGT ACAACAATCT AATACACCAA AGGCCAGAGA GGTCGACAAC AGACAAGGAT 
AAAGATAAGA GATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015807048.1
CDS	1385..5599
Translation	MSDTQPSFTDRFNKKAIRTSSILSKDHPPDKKPKSDKTSPPSNEFDPTGLVQRCVIIQKDENGFGFTVSGDNPVFVQLVKEDGAAMRAGVQTGDRIIKVNGTLVTQSNHTEVVKLIKSGSYVALTVLGRPPGLAQIPLPEAEPDVFGVRVSSPNSPATERSCSPQDRLFSPQQQYDENNSFCDQRTNVFAKFLSKEQQELQVMKKEYSRNPSPKLLKDIQEATKHIPQLQGQLFKATGTTQEAVSGGDEDNGSISETDWTPPHKGNNCIDLLWNNTPKSRVFEPGSPSTQSPRDSCASPKSSPKDSFNPVYSPETEDVTDLDTVSPTTVSSPLPCRLGLQIIGAEDDYFDSDQEQTNGQCNSFNSIEQLKSRPAHLAAFLHHVISQFDPAPVLCYLYADYYKQTNSKETRKELHNVFIDRAAILKVPIPESVSSDLNWRRMELNPEETNKQYVKTLQDSLLSDIQKNLEDFRQKRSMGLTVAKAELTRLDQERVRDRVTIERERSYAESIISKIDDILFTTQTTEEDKCTTIQNVIYTYMKHLGVRVKEPRILEAKRGRKSFLPKMMQKGIKTEKEGEDKGRKVFMLPLRRPSRIDSSPAIKALDSRPKPQKQLSQPTPVTSELMEVGPLRGSQSSDCNDLNHSMSVTTSSPNINTHCTDVSRDVDIGGTSSGSSRLNDGLQTDLDVFPFPSSPLDVCDQLQEDDSDRAHEGTPKATRRFEGLIAADVQSEDDQGTDSEHNPPNWHQRVGREVLVGLSPHEIKRQEVINELFYTERAHVRMLKVLDLVFYQKLSKDAILPPTDIKNIFTNLEEVLQLHVSILEQMTAVRKRNESSVIDQIGDDLLSWFSGGEEEKIKQAVGTFCSNQPFALEIIKTKQKKDSRFISFIQEAESNRLCRRLQLKDLIPVEMLRLTKYPLLLDNIVKYTDIAVEKQKVKQAAECCRRILSHVNQAVKESEDKQKLEDYQRRLDLSTLKQTDNPLIAELKNLDLTKRTMIHEGSLSWKVNRDKTIELFTLLLEDILVLLQKQDERLILKCHSKNLAGAADTKHIYSPIIKLSTVLVRSVATDNRSFFVLSVSENGSHIYELMAPTVTDQNTWQSLISQRADAMKVKPHSIIPLPQPDGERDGAEIITAGTSRLGKDPDRTSTSSTQSTDKERGPASRGALQTSLPGSNPFEEVKAEEEEEEGGFVDRVLPYQVAEIDREGDSFDVFPSRADEALKTLAALKHVLVTQLMSQEAAEQSKRSSGVRLLRTTSLRTPTDNRSKVLVHNGSEKSSSKTEDLAQDLGSWDTGFFESPEDYGCLVLEGYCGPRESSTDDDILASTTGKQLNTSQVCAADSGINLRFSSTSQVGSLSTFSRQVLSHLRNLQTNLNYLKEVEAKYNNLIHQRPERSTTDKDKDKR

Target ORF information:

RefSeq Version	XM_015951562.1
Organism	Nothobranchius furzeri(turquoise killifish)
Definition	Nothobranchius furzeri Rho guanine nucleotide exchange factor 12 (arhgef12), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015951562.1

ORF Insert Sequence:

ATGAGTGACA CACAGCCCAG CTTCACTGAC AGGTTTAACA AGAAAGCAAT CAGAACCAGC 
AGCATTCTCA GTAAAGACCA CCCCCCAGAC AAGAAACCCA AAAGTGACAA AACCTCACCT 
CCCTCCAATG AGTTTGACCC TACAGGTCTA GTCCAGCGAT GTGTGATCAT CCAGAAAGAT 
GAAAATGGCT TTGGGTTCAC TGTCAGTGGC GATAACCCCG TGTTTGTGCA GCTGGTCAAA 
GAAGATGGGG CTGCTATGCG GGCAGGTGTT CAGACAGGAG ACAGGATCAT CAAGGTTAAC 
GGGACCCTTG TGACACAGTC CAATCACACT GAAGTTGTGA AACTTATCAA ATCGGGCTCG 
TATGTGGCCC TCACGGTTTT GGGGCGACCT CCGGGGCTCG CTCAGATCCC GTTGCCTGAG 
GCAGAGCCTG ATGTGTTCGG CGTTAGGGTA TCCTCCCCGA ACTCTCCAGC CACCGAACGC 
TCATGCAGTC CTCAGGACCG CCTCTTTTCC CCACAACAAC AATATGACGA GAACAACAGT 
TTCTGCGACC AGAGGACAAA TGTCTTTGCA AAGTTTCTAT CAAAGGAGCA GCAGGAGCTG 
CAGGTCATGA AAAAGGAGTA CAGCAGGAAC CCCTCCCCCA AACTACTGAA AGACATTCAG 
GAAGCTACAA AACACATTCC ACAGCTGCAG GGTCAGCTCT TCAAGGCCAC TGGGACAACA 
CAGGAGGCTG TTTCCGGTGG TGATGAAGAC AATGGTAGCA TATCAGAGAC AGATTGGACC 
CCTCCTCATA AAGGAAACAA CTGCATAGAC CTGCTGTGGA ACAACACTCC AAAATCCCGT 
GTATTCGAAC CTGGATCTCC TTCTACCCAG AGTCCAAGAG ACTCCTGCGC GAGCCCCAAA 
AGCTCTCCTA AAGACAGCTT CAACCCCGTC TACTCCCCAG AGACGGAGGA CGTCACTGAC 
CTGGACACCG TCTCTCCCAC CACGGTCAGC AGTCCTCTTC CCTGCCGCCT TGGCTTGCAA 
ATTATAGGAG CAGAAGACGA CTATTTTGAT TCGGACCAGG AGCAGACAAA TGGCCAGTGT 
AACAGCTTTA ACAGTATTGA GCAGCTCAAG TCTCGTCCTG CCCACCTCGC AGCTTTTTTG 
CATCATGTCA TTTCTCAGTT TGACCCTGCT CCTGTGTTGT GCTACCTCTA TGCTGACTAT 
TACAAGCAAA CCAACTCCAA AGAAACAAGA AAGGAGCTCC ATAACGTCTT CATAGACCGG 
GCAGCAATTT TAAAAGTTCC CATTCCTGAA TCAGTTTCTT CTGACCTTAA CTGGCGACGA 
ATGGAACTGA ACCCTGAAGA AACAAACAAA CAGTATGTTA AAACACTGCA GGACTCTCTC 
CTCTCTGACA TTCAGAAAAA CCTCGAGGAC TTCAGGCAGA AACGCAGTAT GGGCCTAACA 
GTGGCTAAGG CTGAGCTGAC AAGACTGGAC CAAGAAAGAG TCAGAGACCG GGTCACCATA 
GAGCGGGAGC GATCATACGC AGAAAGCATC ATCAGTAAAA TAGATGACAT CCTGTTCACT 
ACTCAAACCA CAGAGGAAGA CAAATGCACT ACGATACAGA ATGTTATCTA CACATACATG 
AAGCACCTTG GGGTGAGAGT CAAGGAACCT CGAATTCTGG AGGCCAAACG GGGTCGGAAA 
AGCTTTTTAC CTAAAATGAT GCAGAAAGGC ATAAAAACTG AGAAGGAAGG AGAAGACAAA 
GGCAGAAAAG TATTTATGTT GCCCCTGCGT CGGCCCAGCC GCATTGACTC GTCGCCAGCC 
ATTAAAGCCC TGGACAGTCG TCCCAAGCCT CAGAAACAGC TGTCTCAGCC GACGCCTGTG 
ACCAGCGAGC TAATGGAAGT TGGTCCATTG AGAGGCAGCC AATCCAGCGA TTGCAATGAC 
CTCAATCACT CCATGTCTGT CACCACCTCA TCTCCAAACA TCAACACCCA CTGCACAGAT 
GTGAGCCGAG ACGTCGACAT CGGTGGGACG TCATCTGGGT CCTCCAGACT GAACGACGGC 
CTTCAGACCG ACCTGGACGT GTTCCCATTC CCGTCTTCAC CACTGGATGT TTGTGATCAG 
CTCCAGGAGG ACGACAGTGA CCGAGCCCAT GAGGGAACTC CAAAGGCCAC AAGAAGATTT 
GAAGGATTGA TTGCAGCTGA TGTCCAAAGT GAGGACGATC AAGGGACAGA CTCTGAGCAC 
AATCCTCCAA ACTGGCATCA GCGGGTGGGT CGAGAAGTCC TCGTAGGCCT CAGTCCTCAT 
GAAATCAAGC GTCAGGAAGT TATCAACGAG CTGTTTTACA CTGAGCGCGC TCATGTGCGG 
ATGCTGAAAG TTTTGGACCT CGTCTTCTAC CAAAAGCTTT CCAAAGATGC AATTCTTCCT 
CCTACTGACA TCAAAAATAT CTTCACCAAC TTGGAGGAGG TTCTGCAGCT GCATGTTTCA 
ATACTGGAGC AAATGACAGC TGTCCGGAAG AGAAACGAGT CTTCCGTCAT TGATCAAATA 
GGAGATGACT TGCTCTCTTG GTTCAGCGGC GGCGAGGAGG AGAAGATCAA GCAGGCAGTG 
GGGACGTTTT GCAGCAATCA GCCTTTTGCT CTAGAGATTA TTAAAACCAA GCAGAAGAAA 
GACTCGAGGT TCATTTCATT CATCCAGGAG GCTGAGAGTA ACAGACTGTG TCGCCGGCTG 
CAGCTCAAAG ACCTCATTCC TGTAGAGATG CTCCGGCTCA CCAAGTACCC GCTGCTACTG 
GACAACATCG TCAAGTACAC AGACATTGCT GTGGAAAAGC AGAAAGTCAA GCAGGCAGCA 
GAATGCTGTA GAAGGATCCT CAGTCATGTC AACCAGGCTG TGAAGGAGTC TGAGGACAAA 
CAGAAATTAG AAGACTATCA GCGAAGATTG GACCTCTCCA CTCTAAAGCA GACTGACAAC 
CCTCTGATCG CTGAACTAAA GAATCTGGAT CTAACAAAGA GGACGATGAT TCATGAGGGT 
TCACTGTCGT GGAAAGTGAA CAGGGACAAG ACTATTGAGT TGTTCACACT CCTCCTTGAG 
GACATCCTCG TTCTGCTGCA GAAACAGGAT GAGCGTCTGA TCCTTAAGTG TCACAGCAAG 
AACCTGGCAG GTGCTGCAGA TACCAAACAC ATCTACAGCC CCATCATCAA ACTCAGCACT 
GTCCTCGTGC GCTCTGTTGC TACGGACAAC AGGTCCTTTT TCGTCCTCTC TGTGTCTGAA 
AACGGTTCCC ACATCTACGA GCTAATGGCG CCCACTGTGA CGGATCAGAA CACGTGGCAA 
AGTCTGATCT CCCAGAGAGC CGATGCAATG AAAGTCAAGC CTCACAGCAT CATCCCGTTA 
CCTCAGCCTG ACGGGGAGAG AGATGGAGCG GAGATCATAA CAGCTGGTAC TTCCCGACTG 
GGTAAAGACC CGGACCGCAC ATCCACCAGC AGCACTCAGT CCACAGATAA AGAGAGAGGT 
CCAGCCTCTA GAGGTGCATT ACAGACTTCT CTACCTGGTA GTAATCCTTT TGAAGAAGTG 
AAGGCTGAGG AGGAGGAGGA GGAGGGTGGC TTTGTGGACC GAGTGCTTCC TTACCAAGTA 
GCTGAAATCG ACAGAGAAGG AGACTCGTTT GATGTGTTTC CCTCTCGAGC AGATGAAGCA 
CTGAAAACAT TGGCAGCATT AAAGCACGTG TTGGTGACCC AGCTGATGTC CCAGGAGGCA 
GCAGAACAAA GCAAACGCTC CTCTGGAGTC CGTCTCCTCA GGACTACGTC CCTGCGGACC 
CCGACGGACA ACCGCAGCAA GGTTCTGGTC CACAACGGCT CCGAGAAAAG TAGCTCCAAA 
ACAGAAGACC TGGCTCAGGA CCTGGGGTCG TGGGACACTG GATTCTTTGA GTCACCTGAA 
GATTACGGAT GTTTGGTCCT AGAAGGTTAC TGCGGCCCAC GGGAGAGCAG CACAGATGAC 
GACATCCTGG CCTCAACCAC AGGGAAGCAG CTGAACACCT CACAAGTCTG CGCTGCCGAC 
TCCGGCATCA ATTTACGATT TTCCTCCACG TCACAGGTCG GCAGCCTCTC CACTTTCAGC 
AGGCAGGTGC TGTCGCACCT TAGAAATCTT CAGACCAACC TCAACTATCT AAAGGAGGTG 
GAGGCGAAGT ACAACAATCT AATACACCAA AGGCCAGAGA GGTCGACAAC AGACAAGGAT 
AAAGATAAGA GATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

arhgef12 ( XM_015951563.1 ) cDNA ORF clone, Nothobranchius furzeri(turquoise killifish) -> XP_015807049.1 Nothobranchius furzeri Rho guanine nucleotide exchange factor 12 (arhgef12), transcript variant X6, mRNA.

Price & Availability↑

CloneID	ONo10375
Clone ID Related Accession (Same CDS sequence)	XM_015951563.1
Accession Version	XM_015951563.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4212bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-03-17
Organism	Nothobranchius furzeri(turquoise killifish)
Product	rho guanine nucleotide exchange factor 12 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015443528.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Nothobranchius furzeri Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGACA CACAGCCCAG CTTCACTGAC AGGTTTAACA AGAAAGCAAT CAGAACCAGC 
AGCATTCTCA GTAAAGACCA CCCCCCAGAC AAGAAACCCA AAAGTGACAA AACCTCACCT 
CCCTCCAATG AGTTTGACCC TACAGGTCTA GTCCAGCGAT GTGTGATCAT CCAGAAAGAT 
GAAAATGGCT TTGGGTTCAC TGTCAGTGGC GATAACCCCG TGTTTGTGCA GCTGGTCAAA 
GAAGATGGGG CTGCTATGCG GGCAGGTGTT CAGACAGGAG ACAGGATCAT CAAGGTTAAC 
GGGACCCTTG TGACACAGTC CAATCACACT GAAGTTGTGA AACTTATCAA ATCGGGCTCG 
TATGTGGCCC TCACGGTTTT GGGGCGACCT CCGGGGCTCG CTCAGATCCC GTTGCCTGAG 
GCAGAGCCTG ATGTGTTCGG CGTTAGGGTA TCCTCCCCGA ACTCTCCAGC CACCGAACGC 
TCATGCAGTC CTCAGGACCG CCTCTTTTCC CCACAACAAC AATATGACGA GAACAACAGT 
TTCTGCGACC AGAGGACAAA TGTCTTTGCA AAGTTTCTAT CAAAGGAGCA GCAGGAGCTG 
CAGGTCATGA AAAAGGAGTA CAGCAGGAAC CCCTCCCCCA AACTACTGAA AGACATTCAG 
GAAGCTACAA AACACATTCC ACAGCTGCAG GGTCAGCTCT TCAAGGCCAC TGGGACAACA 
CAGGAGGCTG TTTCCGGTGG TGATGAAGAC AATGGTAGCA TATCAGAGAC AGATTGGACC 
CCTCCTCATA AAGGAAACAA CTGCATAGAC CTGCTGTGGA ACAACACTCC AAAATCCCGT 
GTATTCGAAC CTGGATCTCC TTCTACCCAG AGTCCAAGAG ACTCCTGCGC GAGCCCCAAA 
AGCTCTCCTA AAGACAGCTT CAACCCCGTC TACTCCCCAG AGACGGAGGA CGTCACTGAC 
CTGGACACCG TCTCTCCCAC CACGGTCAGC AGTCCTCTTC CCTGCCGCCT TGGCTTGCAA 
ATTATAGGAG CAGAAGACGA CTATTTTGAT TCGGACCAGG AGCAGACAAA TGGCCAGTGT 
AACAGCTTTA ACAGTATTGA GCAGCTCAAG TCTCGTCCTG CCCACCTCGC AGCTTTTTTG 
CATCATGTCA TTTCTCAGTT TGACCCTGCT CCTGTGTTGT GCTACCTCTA TGCTGACTAT 
TACAAGCAAA CCAACTCCAA AGAAACAAGA AAGGAGCTCC ATAACGTCTT CATAGACCGG 
GCAGCAATTT TAAAAGTTCC CATTCCTGAA TCAGTTTCTT CTGACCTTAA CTGGCGACGA 
ATGGAACTGA ACCCTGAAGA AACAAACAAA CAGTATGTTA AAACACTGCA GGACTCTCTC 
CTCTCTGACA TTCAGAAAAA CCTCGAGGAC TTCAGGCAGA AACGCAGTAT GGGCCTAACA 
GTGGCTAAGG CTGAGCTGAC AAGACTGGAC CAAGAAAGAG TCAGAGACCG GGTCACCATA 
GAGCGGGAGC GATCATACGC AGAAAGCATC ATCAGTAAAA TAGATGACAT CCTGTTCACT 
ACTCAAACCA CAGAGGAAGA CAAATGCACT ACGATACAGA ATGTTATCTA CACATACATG 
AAGCACCTTG GGGTGAGAGT CAAGGAACCT CGAATTCTGG AGGCCAAACG GGGTCGGAAA 
AGCTTTTTAC CTAAAATGAT GAAAGGCATA AAAACTGAGA AGGAAGGAGA AGACAAAGGC 
AGAAAAGTAT TTATGTTGCC CCTGCGTCGG CCCAGCCGCA TTGACTCGTC GCCAGCCATT 
AAAGCCCTGG ACAGTCGTCC CAAGCCTCAG AAACAGCTGT CTCAGCCGAC GCCTGTGACC 
AGCGAGCTAA TGGAAGTTGG TCCATTGAGA GGCAGCCAAT CCAGCGATTG CAATGACCTC 
AATCACTCCA TGTCTGTCAC CACCTCATCT CCAAACATCA ACACCCACTG CACAGATGTG 
AGCCGAGACG TCGACATCGG TGGGACGTCA TCTGGGTCCT CCAGACTGAA CGACGGCCTT 
CAGACCGACC TGGACGTGTT CCCATTCCCG TCTTCACCAC TGGATGTTTG TGATCAGCTC 
CAGGAGGACG ACAGTGACCG AGCCCATGAG GGAACTCCAA AGGCCACAAG AAGATTTGAA 
GGATTGATTG CAGCTGATGT CCAAAGTGAG GACGATCAAG GGACAGACTC TGAGCACAAT 
CCTCCAAACT GGCATCAGCG GGTGGGTCGA GAAGTCCTCG TAGGCCTCAG TCCTCATGAA 
ATCAAGCGTC AGGAAGTTAT CAACGAGCTG TTTTACACTG AGCGCGCTCA TGTGCGGATG 
CTGAAAGTTT TGGACCTCGT CTTCTACCAA AAGCTTTCCA AAGATGCAAT TCTTCCTCCT 
ACTGACATCA AAAATATCTT CACCAACTTG GAGGAGGTTC TGCAGCTGCA TGTTTCAATA 
CTGGAGCAAA TGACAGCTGT CCGGAAGAGA AACGAGTCTT CCGTCATTGA TCAAATAGGA 
GATGACTTGC TCTCTTGGTT CAGCGGCGGC GAGGAGGAGA AGATCAAGCA GGCAGTGGGG 
ACGTTTTGCA GCAATCAGCC TTTTGCTCTA GAGATTATTA AAACCAAGCA GAAGAAAGAC 
TCGAGGTTCA TTTCATTCAT CCAGGAGGCT GAGAGTAACA GACTGTGTCG CCGGCTGCAG 
CTCAAAGACC TCATTCCTGT AGAGATGCTC CGGCTCACCA AGTACCCGCT GCTACTGGAC 
AACATCGTCA AGTACACAGA CATTGCTGTG GAAAAGCAGA AAGTCAAGCA GGCAGCAGAA 
TGCTGTAGAA GGATCCTCAG TCATGTCAAC CAGGCTGTGA AGGAGTCTGA GGACAAACAG 
AAATTAGAAG ACTATCAGCG AAGATTGGAC CTCTCCACTC TAAAGCAGAC TGACAACCCT 
CTGATCGCTG AACTAAAGAA TCTGGATCTA ACAAAGAGGA CGATGATTCA TGAGGGTTCA 
CTGTCGTGGA AAGTGAACAG GGACAAGACT ATTGAGTTGT TCACACTCCT CCTTGAGGAC 
ATCCTCGTTC TGCTGCAGAA ACAGGATGAG CGTCTGATCC TTAAGTGTCA CAGCAAGAAC 
CTGGCAGGTG CTGCAGATAC CAAACACATC TACAGCCCCA TCATCAAACT CAGCACTGTC 
CTCGTGCGCT CTGTTGCTAC GGACAACAGG TCCTTTTTCG TCCTCTCTGT GTCTGAAAAC 
GGTTCCCACA TCTACGAGCT AATGGCGCCC ACTGTGACGG ATCAGAACAC GTGGCAAAGT 
CTGATCTCCC AGAGAGCCGA TGCAATGAAA GTCAAGCCTC ACAGCATCAT CCCGTTACCT 
CAGCCTGACG GGGAGAGAGA TGGAGCGGAG ATCATAACAG CTGGTACTTC CCGACTGGGT 
AAAGACCCGG ACCGCACATC CACCAGCAGC ACTCAGTCCA CAGATAAAGA GAGAGGTCCA 
GCCTCTAGAG GTGCATTACA GACTTCTCTA CCTGGTAGTA ATCCTTTTGA AGAAGTGAAG 
GCTGAGGAGG AGGAGGAGGA GGGTGGCTTT GTGGACCGAG TGCTTCCTTA CCAAGTAGCT 
GAAATCGACA GAGAAGGAGA CTCGTTTGAT GTGTTTCCCT CTCGAGCAGA TGAAGCACTG 
AAAACATTGG CAGCATTAAA GCACGTGTTG GTGACCCAGC TGATGTCCCA GGAGGCAGCA 
GAACAAAGCA AACGCTCCTC TGGAGTCCGT CTCCTCAGGA CTACGTCCCT GCGGACCCCG 
ACGGACAACC GCAGCAAGGT TCTGGTCCAC AACGGCTCCG AGAAAAGTAG CTCCAAAACA 
GAAGACCTGG CTCAGGACCT GGGGTCGTGG GACACTGGAT TCTTTGAGTC ACCTGAAGAT 
TACGGATGTT TGGTCCTAGA AGGTTACTGC GGCCCACGGG AGAGCAGCAC AGATGACGAC 
ATCCTGGCCT CAACCACAGG GAAGCAGCTG AACACCTCAC AAGTCTGCGC TGCCGACTCC 
GGCATCAATT TACGATTTTC CTCCACGTCA CAGGTCGGCA GCCTCTCCAC TTTCAGCAGG 
CAGGTGCTGT CGCACCTTAG AAATCTTCAG ACCAACCTCA ACTATCTAAA GGAGGTGGAG 
GCGAAGTACA ACAATCTAAT ACACCAAAGG CCAGAGAGGT CGACAACAGA CAAGGATAAA 
GATAAGAGAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015807049.1
CDS	1385..5596
Translation	MSDTQPSFTDRFNKKAIRTSSILSKDHPPDKKPKSDKTSPPSNEFDPTGLVQRCVIIQKDENGFGFTVSGDNPVFVQLVKEDGAAMRAGVQTGDRIIKVNGTLVTQSNHTEVVKLIKSGSYVALTVLGRPPGLAQIPLPEAEPDVFGVRVSSPNSPATERSCSPQDRLFSPQQQYDENNSFCDQRTNVFAKFLSKEQQELQVMKKEYSRNPSPKLLKDIQEATKHIPQLQGQLFKATGTTQEAVSGGDEDNGSISETDWTPPHKGNNCIDLLWNNTPKSRVFEPGSPSTQSPRDSCASPKSSPKDSFNPVYSPETEDVTDLDTVSPTTVSSPLPCRLGLQIIGAEDDYFDSDQEQTNGQCNSFNSIEQLKSRPAHLAAFLHHVISQFDPAPVLCYLYADYYKQTNSKETRKELHNVFIDRAAILKVPIPESVSSDLNWRRMELNPEETNKQYVKTLQDSLLSDIQKNLEDFRQKRSMGLTVAKAELTRLDQERVRDRVTIERERSYAESIISKIDDILFTTQTTEEDKCTTIQNVIYTYMKHLGVRVKEPRILEAKRGRKSFLPKMMKGIKTEKEGEDKGRKVFMLPLRRPSRIDSSPAIKALDSRPKPQKQLSQPTPVTSELMEVGPLRGSQSSDCNDLNHSMSVTTSSPNINTHCTDVSRDVDIGGTSSGSSRLNDGLQTDLDVFPFPSSPLDVCDQLQEDDSDRAHEGTPKATRRFEGLIAADVQSEDDQGTDSEHNPPNWHQRVGREVLVGLSPHEIKRQEVINELFYTERAHVRMLKVLDLVFYQKLSKDAILPPTDIKNIFTNLEEVLQLHVSILEQMTAVRKRNESSVIDQIGDDLLSWFSGGEEEKIKQAVGTFCSNQPFALEIIKTKQKKDSRFISFIQEAESNRLCRRLQLKDLIPVEMLRLTKYPLLLDNIVKYTDIAVEKQKVKQAAECCRRILSHVNQAVKESEDKQKLEDYQRRLDLSTLKQTDNPLIAELKNLDLTKRTMIHEGSLSWKVNRDKTIELFTLLLEDILVLLQKQDERLILKCHSKNLAGAADTKHIYSPIIKLSTVLVRSVATDNRSFFVLSVSENGSHIYELMAPTVTDQNTWQSLISQRADAMKVKPHSIIPLPQPDGERDGAEIITAGTSRLGKDPDRTSTSSTQSTDKERGPASRGALQTSLPGSNPFEEVKAEEEEEEGGFVDRVLPYQVAEIDREGDSFDVFPSRADEALKTLAALKHVLVTQLMSQEAAEQSKRSSGVRLLRTTSLRTPTDNRSKVLVHNGSEKSSSKTEDLAQDLGSWDTGFFESPEDYGCLVLEGYCGPRESSTDDDILASTTGKQLNTSQVCAADSGINLRFSSTSQVGSLSTFSRQVLSHLRNLQTNLNYLKEVEAKYNNLIHQRPERSTTDKDKDKR

Target ORF information:

RefSeq Version	XM_015951563.1
Organism	Nothobranchius furzeri(turquoise killifish)
Definition	Nothobranchius furzeri Rho guanine nucleotide exchange factor 12 (arhgef12), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015951563.1

ORF Insert Sequence:

ATGAGTGACA CACAGCCCAG CTTCACTGAC AGGTTTAACA AGAAAGCAAT CAGAACCAGC 
AGCATTCTCA GTAAAGACCA CCCCCCAGAC AAGAAACCCA AAAGTGACAA AACCTCACCT 
CCCTCCAATG AGTTTGACCC TACAGGTCTA GTCCAGCGAT GTGTGATCAT CCAGAAAGAT 
GAAAATGGCT TTGGGTTCAC TGTCAGTGGC GATAACCCCG TGTTTGTGCA GCTGGTCAAA 
GAAGATGGGG CTGCTATGCG GGCAGGTGTT CAGACAGGAG ACAGGATCAT CAAGGTTAAC 
GGGACCCTTG TGACACAGTC CAATCACACT GAAGTTGTGA AACTTATCAA ATCGGGCTCG 
TATGTGGCCC TCACGGTTTT GGGGCGACCT CCGGGGCTCG CTCAGATCCC GTTGCCTGAG 
GCAGAGCCTG ATGTGTTCGG CGTTAGGGTA TCCTCCCCGA ACTCTCCAGC CACCGAACGC 
TCATGCAGTC CTCAGGACCG CCTCTTTTCC CCACAACAAC AATATGACGA GAACAACAGT 
TTCTGCGACC AGAGGACAAA TGTCTTTGCA AAGTTTCTAT CAAAGGAGCA GCAGGAGCTG 
CAGGTCATGA AAAAGGAGTA CAGCAGGAAC CCCTCCCCCA AACTACTGAA AGACATTCAG 
GAAGCTACAA AACACATTCC ACAGCTGCAG GGTCAGCTCT TCAAGGCCAC TGGGACAACA 
CAGGAGGCTG TTTCCGGTGG TGATGAAGAC AATGGTAGCA TATCAGAGAC AGATTGGACC 
CCTCCTCATA AAGGAAACAA CTGCATAGAC CTGCTGTGGA ACAACACTCC AAAATCCCGT 
GTATTCGAAC CTGGATCTCC TTCTACCCAG AGTCCAAGAG ACTCCTGCGC GAGCCCCAAA 
AGCTCTCCTA AAGACAGCTT CAACCCCGTC TACTCCCCAG AGACGGAGGA CGTCACTGAC 
CTGGACACCG TCTCTCCCAC CACGGTCAGC AGTCCTCTTC CCTGCCGCCT TGGCTTGCAA 
ATTATAGGAG CAGAAGACGA CTATTTTGAT TCGGACCAGG AGCAGACAAA TGGCCAGTGT 
AACAGCTTTA ACAGTATTGA GCAGCTCAAG TCTCGTCCTG CCCACCTCGC AGCTTTTTTG 
CATCATGTCA TTTCTCAGTT TGACCCTGCT CCTGTGTTGT GCTACCTCTA TGCTGACTAT 
TACAAGCAAA CCAACTCCAA AGAAACAAGA AAGGAGCTCC ATAACGTCTT CATAGACCGG 
GCAGCAATTT TAAAAGTTCC CATTCCTGAA TCAGTTTCTT CTGACCTTAA CTGGCGACGA 
ATGGAACTGA ACCCTGAAGA AACAAACAAA CAGTATGTTA AAACACTGCA GGACTCTCTC 
CTCTCTGACA TTCAGAAAAA CCTCGAGGAC TTCAGGCAGA AACGCAGTAT GGGCCTAACA 
GTGGCTAAGG CTGAGCTGAC AAGACTGGAC CAAGAAAGAG TCAGAGACCG GGTCACCATA 
GAGCGGGAGC GATCATACGC AGAAAGCATC ATCAGTAAAA TAGATGACAT CCTGTTCACT 
ACTCAAACCA CAGAGGAAGA CAAATGCACT ACGATACAGA ATGTTATCTA CACATACATG 
AAGCACCTTG GGGTGAGAGT CAAGGAACCT CGAATTCTGG AGGCCAAACG GGGTCGGAAA 
AGCTTTTTAC CTAAAATGAT GAAAGGCATA AAAACTGAGA AGGAAGGAGA AGACAAAGGC 
AGAAAAGTAT TTATGTTGCC CCTGCGTCGG CCCAGCCGCA TTGACTCGTC GCCAGCCATT 
AAAGCCCTGG ACAGTCGTCC CAAGCCTCAG AAACAGCTGT CTCAGCCGAC GCCTGTGACC 
AGCGAGCTAA TGGAAGTTGG TCCATTGAGA GGCAGCCAAT CCAGCGATTG CAATGACCTC 
AATCACTCCA TGTCTGTCAC CACCTCATCT CCAAACATCA ACACCCACTG CACAGATGTG 
AGCCGAGACG TCGACATCGG TGGGACGTCA TCTGGGTCCT CCAGACTGAA CGACGGCCTT 
CAGACCGACC TGGACGTGTT CCCATTCCCG TCTTCACCAC TGGATGTTTG TGATCAGCTC 
CAGGAGGACG ACAGTGACCG AGCCCATGAG GGAACTCCAA AGGCCACAAG AAGATTTGAA 
GGATTGATTG CAGCTGATGT CCAAAGTGAG GACGATCAAG GGACAGACTC TGAGCACAAT 
CCTCCAAACT GGCATCAGCG GGTGGGTCGA GAAGTCCTCG TAGGCCTCAG TCCTCATGAA 
ATCAAGCGTC AGGAAGTTAT CAACGAGCTG TTTTACACTG AGCGCGCTCA TGTGCGGATG 
CTGAAAGTTT TGGACCTCGT CTTCTACCAA AAGCTTTCCA AAGATGCAAT TCTTCCTCCT 
ACTGACATCA AAAATATCTT CACCAACTTG GAGGAGGTTC TGCAGCTGCA TGTTTCAATA 
CTGGAGCAAA TGACAGCTGT CCGGAAGAGA AACGAGTCTT CCGTCATTGA TCAAATAGGA 
GATGACTTGC TCTCTTGGTT CAGCGGCGGC GAGGAGGAGA AGATCAAGCA GGCAGTGGGG 
ACGTTTTGCA GCAATCAGCC TTTTGCTCTA GAGATTATTA AAACCAAGCA GAAGAAAGAC 
TCGAGGTTCA TTTCATTCAT CCAGGAGGCT GAGAGTAACA GACTGTGTCG CCGGCTGCAG 
CTCAAAGACC TCATTCCTGT AGAGATGCTC CGGCTCACCA AGTACCCGCT GCTACTGGAC 
AACATCGTCA AGTACACAGA CATTGCTGTG GAAAAGCAGA AAGTCAAGCA GGCAGCAGAA 
TGCTGTAGAA GGATCCTCAG TCATGTCAAC CAGGCTGTGA AGGAGTCTGA GGACAAACAG 
AAATTAGAAG ACTATCAGCG AAGATTGGAC CTCTCCACTC TAAAGCAGAC TGACAACCCT 
CTGATCGCTG AACTAAAGAA TCTGGATCTA ACAAAGAGGA CGATGATTCA TGAGGGTTCA 
CTGTCGTGGA AAGTGAACAG GGACAAGACT ATTGAGTTGT TCACACTCCT CCTTGAGGAC 
ATCCTCGTTC TGCTGCAGAA ACAGGATGAG CGTCTGATCC TTAAGTGTCA CAGCAAGAAC 
CTGGCAGGTG CTGCAGATAC CAAACACATC TACAGCCCCA TCATCAAACT CAGCACTGTC 
CTCGTGCGCT CTGTTGCTAC GGACAACAGG TCCTTTTTCG TCCTCTCTGT GTCTGAAAAC 
GGTTCCCACA TCTACGAGCT AATGGCGCCC ACTGTGACGG ATCAGAACAC GTGGCAAAGT 
CTGATCTCCC AGAGAGCCGA TGCAATGAAA GTCAAGCCTC ACAGCATCAT CCCGTTACCT 
CAGCCTGACG GGGAGAGAGA TGGAGCGGAG ATCATAACAG CTGGTACTTC CCGACTGGGT 
AAAGACCCGG ACCGCACATC CACCAGCAGC ACTCAGTCCA CAGATAAAGA GAGAGGTCCA 
GCCTCTAGAG GTGCATTACA GACTTCTCTA CCTGGTAGTA ATCCTTTTGA AGAAGTGAAG 
GCTGAGGAGG AGGAGGAGGA GGGTGGCTTT GTGGACCGAG TGCTTCCTTA CCAAGTAGCT 
GAAATCGACA GAGAAGGAGA CTCGTTTGAT GTGTTTCCCT CTCGAGCAGA TGAAGCACTG 
AAAACATTGG CAGCATTAAA GCACGTGTTG GTGACCCAGC TGATGTCCCA GGAGGCAGCA 
GAACAAAGCA AACGCTCCTC TGGAGTCCGT CTCCTCAGGA CTACGTCCCT GCGGACCCCG 
ACGGACAACC GCAGCAAGGT TCTGGTCCAC AACGGCTCCG AGAAAAGTAG CTCCAAAACA 
GAAGACCTGG CTCAGGACCT GGGGTCGTGG GACACTGGAT TCTTTGAGTC ACCTGAAGAT 
TACGGATGTT TGGTCCTAGA AGGTTACTGC GGCCCACGGG AGAGCAGCAC AGATGACGAC 
ATCCTGGCCT CAACCACAGG GAAGCAGCTG AACACCTCAC AAGTCTGCGC TGCCGACTCC 
GGCATCAATT TACGATTTTC CTCCACGTCA CAGGTCGGCA GCCTCTCCAC TTTCAGCAGG 
CAGGTGCTGT CGCACCTTAG AAATCTTCAG ACCAACCTCA ACTATCTAAA GGAGGTGGAG 
GCGAAGTACA ACAATCTAAT ACACCAAAGG CCAGAGAGGT CGACAACAGA CAAGGATAAA 
GATAAGAGAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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03-6811-6572

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オンライン注文

arhgef12 cDNA ORF clone, Nothobranchius furzeri(turquoise killifish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム