mut cDNA ORF clone, Nothobranchius furzeri(turquoise killifish)

Gene
Clones
gRNAs

Summary

Gene Symbol	mut
Entrez Gene ID	107378132
Full Name	methylmalonyl-CoA mutase
Gene Type	protein-coding
Organism	Nothobranchius furzeri(turquoise killifish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015948176.1	XP_015803662.1	methylmalonyl-CoA mutase, mitochondrial

Pathways from BioSystems
Gene Ontology

The following mut gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the mut cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ONo07645	XM_015948176.1 Latest version!	Nothobranchius furzeri methylmalonyl-CoA mutase (mut), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

mut ( XM_015948176.1 ) cDNA ORF clone, Nothobranchius furzeri(turquoise killifish) -> XP_015803662.1 Nothobranchius furzeri methylmalonyl-CoA mutase (mut), mRNA.

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CloneID	ONo07645
Clone ID Related Accession (Same CDS sequence)	XM_015948176.1
Accession Version	XM_015948176.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2259bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-03-17
Organism	Nothobranchius furzeri(turquoise killifish)
Product	methylmalonyl-CoA mutase, mitochondrial
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015443511.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Nothobranchius furzeri Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGACGG CACACAGGGC ATGTGCAGCA CTGGCTGTAC GAAGCTTGTT TGGAGCTCCA 
TTGTCCTACA CACACGGCTT TCAAGTTCAC ACTTCAGTAG CACGTCCGGA CCGGGTTGAG 
CTGCACCCAG AGTGGGCCAG CTTGGCTAAG AAACAGCTGA AGGGAAAGAA CCCAGAAGAC 
CTCATCTGGC ACACTCCAGA GGGAATCAGC ATCAAACCAG TATACACCAA GAAAGACTCA 
GAAAAACTTG CTGATGAACT GCCGGGAGTG TTTCCCTACA CTAGAGGGCC CTACCCCACC 
ATGTACACTT ACAGACCTTG GACAATTCGA CAGTATGCCG GCTTCAGCAC CGTGGAGGAG 
AGCAACAAGT TTTATAAAGA TAACATTAAA GCTGGTCAGC AGGGTCTGTC TGTGGCCTTT 
GACCTCCCAA CGCACCGCGG TTATGACTCA GACAACCCCA GGGTTCACGG TGACGTGGGC 
ATGGCTGGAG TAGCTATAGA CACGGTGGAG GACATGAAGA TGCTCTTTGA CGGAATCCCC 
CTGGAGAAGA TGTCCGTCTC CATGACGATG AACGGAGCTG TCATTCCTGT GCTGGCCATG 
TTTATTGTCA CCGGGGAGGA GCAGGGTGTT CCAAAGGAAA AACTAACTGG CACCATTCAG 
AATGATATTT TAAAAGAGTT CATGGTACGA AACACCTACA TTTTCCCCCC GGAGCCTTCC 
ATGCATGCCA TTGCAGACAT TTTCGCCTAC ACCTCTAAGC ACATGCCCAA GTTTAACTCC 
ATATCCATCA GTGGCTACCA TCTTCAGGAG GCTGGTGCCG ATGCTATCTT AGAGGTAGCT 
TACACCATAG CTAACGGTCT GGAGTACTGT CGAACGGGTC TGAAAGCAGG TCTGACCATC 
GATGAGTTTG CTCCAAGGTT GTCCTTCTTC TGGGGTATAG GGATGAATTT CTACATGGAA 
ATAGCCAAGC TGAGGGCAGC CAGGAGGTTA TGGGCCACTC TGATTAAAGA AAACTTCCAG 
CCCAAGAACA CCAAGTCCCT CCTGCTGCGC ACCCACTGCC AGACGTCAGG CTGGTCTCTC 
ACAGAGCAAG ATCCGTACAA CAATGTGATT CGTACCGTAA TCGAAGCCAT GGCTGCTGTG 
TTTGGCGGCA CCCAGTCGCT ACACACCAAC TCTTTTGATG AAGCTCTGGG ATTACCAACG 
GTGAAGAGTG CCCGCATCGC TAGAAACACC CAGATCATTG TCCAAGAAGA GTCAGGCATC 
CCTAAGGTGG CCGATCCCTG GGGGGGCTCA CACATGATGG AAGCTCTGAC TGATGACGTC 
TACAATACTG CTCTGAAGTT CATTAAAGAC ATCGAAGAGA TGGGAGGCAT GGCCAAAGCT 
GTGGCAGAGG GCATTCCAAA ACTTCGGATT GAGGAATGTG CGGCACGAAG ACAGGCTCGC 
ATTGACTCAG GGTCAGAAGT CATTGTTGGG GTGAACAAGT ATCGTCTGGA GAAGGAGGAG 
ACCGTGGAGG TGCTCGCCAT AGACAACACC TCAGTTCGTC AGAGACAGAT TGAAAAGCTA 
AACAAGGTGA AGGAGTGTCG TGATGCTGAA GCTGCACTGA AGTGCCTGGT GGACATAGAG 
GAGTGTGCTC GAACCAGGAA GGGGAACCTC CTGGCTCTAG CTGTGGAGGC AGCACGAGTC 
AGATGCACGG TTGGTGAAAT CACTGACGCA ATGAAGAAAG TGTTTGGTGA ACACAAGGCC 
AGCACCAGGA TGGTGAGTGG AGCTTACCGC AGCGAGTTTG GTGAGCATGA GGAGATCTCC 
GTGGCCCACG GCAGAGTTGC AGAATTTAAA AGCCAAGAGG GCAGGAACCC TCGGCTCCTG 
GTGGCAAAGA TGGGGCAGGA TGGCCATGAC AGAGGAGCTA AAGTCATCGC CACTGGCTTT 
GCAGACTTGG GCTTTGATGT GGACATTGGA CCACTGTTCC AGACTCCCGT TGAGGTGGCC 
CAGCAGGCCG TGGACGCAGA CGTCCACTGT GTTGGAGTCA GCACGCTCGC TGCCGGACAC 
AAGACCCTGG TCCCAGAGCT CATCAAGGAG CTGCGGAAGC TCAACAGGCC AGATATCCTC 
GTTATCTGCG GAGGAGTCAT CCCACCACAG GACTACGAGT TCCTGTATCA GAGTGGTGTG 
TGTGCCATCT TCGGCCCTGG AACCAGGATC CCACAAGCTG CCGTCGAAGT CATCGACAAC 
ATAGAGAAGA GTTTGGAGAA TATACGGCAA GCCATGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015803662.1
CDS	150..2408
Translation	MLTAHRACAALAVRSLFGAPLSYTHGFQVHTSVARPDRVELHPEWASLAKKQLKGKNPEDLIWHTPEGISIKPVYTKKDSEKLADELPGVFPYTRGPYPTMYTYRPWTIRQYAGFSTVEESNKFYKDNIKAGQQGLSVAFDLPTHRGYDSDNPRVHGDVGMAGVAIDTVEDMKMLFDGIPLEKMSVSMTMNGAVIPVLAMFIVTGEEQGVPKEKLTGTIQNDILKEFMVRNTYIFPPEPSMHAIADIFAYTSKHMPKFNSISISGYHLQEAGADAILEVAYTIANGLEYCRTGLKAGLTIDEFAPRLSFFWGIGMNFYMEIAKLRAARRLWATLIKENFQPKNTKSLLLRTHCQTSGWSLTEQDPYNNVIRTVIEAMAAVFGGTQSLHTNSFDEALGLPTVKSARIARNTQIIVQEESGIPKVADPWGGSHMMEALTDDVYNTALKFIKDIEEMGGMAKAVAEGIPKLRIEECAARRQARIDSGSEVIVGVNKYRLEKEETVEVLAIDNTSVRQRQIEKLNKVKECRDAEAALKCLVDIEECARTRKGNLLALAVEAARVRCTVGEITDAMKKVFGEHKASTRMVSGAYRSEFGEHEEISVAHGRVAEFKSQEGRNPRLLVAKMGQDGHDRGAKVIATGFADLGFDVDIGPLFQTPVEVAQQAVDADVHCVGVSTLAAGHKTLVPELIKELRKLNRPDILVICGGVIPPQDYEFLYQSGVCAIFGPGTRIPQAAVEVIDNIEKSLENIRQAM

Target ORF information:

RefSeq Version	XM_015948176.1
Organism	Nothobranchius furzeri(turquoise killifish)
Definition	Nothobranchius furzeri methylmalonyl-CoA mutase (mut), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015948176.1

ORF Insert Sequence:

ATGCTGACGG CACACAGGGC ATGTGCAGCA CTGGCTGTAC GAAGCTTGTT TGGAGCTCCA 
TTGTCCTACA CACACGGCTT TCAAGTTCAC ACTTCAGTAG CACGTCCGGA CCGGGTTGAG 
CTGCACCCAG AGTGGGCCAG CTTGGCTAAG AAACAGCTGA AGGGAAAGAA CCCAGAAGAC 
CTCATCTGGC ACACTCCAGA GGGAATCAGC ATCAAACCAG TATACACCAA GAAAGACTCA 
GAAAAACTTG CTGATGAACT GCCGGGAGTG TTTCCCTACA CTAGAGGGCC CTACCCCACC 
ATGTACACTT ACAGACCTTG GACAATTCGA CAGTATGCCG GCTTCAGCAC CGTGGAGGAG 
AGCAACAAGT TTTATAAAGA TAACATTAAA GCTGGTCAGC AGGGTCTGTC TGTGGCCTTT 
GACCTCCCAA CGCACCGCGG TTATGACTCA GACAACCCCA GGGTTCACGG TGACGTGGGC 
ATGGCTGGAG TAGCTATAGA CACGGTGGAG GACATGAAGA TGCTCTTTGA CGGAATCCCC 
CTGGAGAAGA TGTCCGTCTC CATGACGATG AACGGAGCTG TCATTCCTGT GCTGGCCATG 
TTTATTGTCA CCGGGGAGGA GCAGGGTGTT CCAAAGGAAA AACTAACTGG CACCATTCAG 
AATGATATTT TAAAAGAGTT CATGGTACGA AACACCTACA TTTTCCCCCC GGAGCCTTCC 
ATGCATGCCA TTGCAGACAT TTTCGCCTAC ACCTCTAAGC ACATGCCCAA GTTTAACTCC 
ATATCCATCA GTGGCTACCA TCTTCAGGAG GCTGGTGCCG ATGCTATCTT AGAGGTAGCT 
TACACCATAG CTAACGGTCT GGAGTACTGT CGAACGGGTC TGAAAGCAGG TCTGACCATC 
GATGAGTTTG CTCCAAGGTT GTCCTTCTTC TGGGGTATAG GGATGAATTT CTACATGGAA 
ATAGCCAAGC TGAGGGCAGC CAGGAGGTTA TGGGCCACTC TGATTAAAGA AAACTTCCAG 
CCCAAGAACA CCAAGTCCCT CCTGCTGCGC ACCCACTGCC AGACGTCAGG CTGGTCTCTC 
ACAGAGCAAG ATCCGTACAA CAATGTGATT CGTACCGTAA TCGAAGCCAT GGCTGCTGTG 
TTTGGCGGCA CCCAGTCGCT ACACACCAAC TCTTTTGATG AAGCTCTGGG ATTACCAACG 
GTGAAGAGTG CCCGCATCGC TAGAAACACC CAGATCATTG TCCAAGAAGA GTCAGGCATC 
CCTAAGGTGG CCGATCCCTG GGGGGGCTCA CACATGATGG AAGCTCTGAC TGATGACGTC 
TACAATACTG CTCTGAAGTT CATTAAAGAC ATCGAAGAGA TGGGAGGCAT GGCCAAAGCT 
GTGGCAGAGG GCATTCCAAA ACTTCGGATT GAGGAATGTG CGGCACGAAG ACAGGCTCGC 
ATTGACTCAG GGTCAGAAGT CATTGTTGGG GTGAACAAGT ATCGTCTGGA GAAGGAGGAG 
ACCGTGGAGG TGCTCGCCAT AGACAACACC TCAGTTCGTC AGAGACAGAT TGAAAAGCTA 
AACAAGGTGA AGGAGTGTCG TGATGCTGAA GCTGCACTGA AGTGCCTGGT GGACATAGAG 
GAGTGTGCTC GAACCAGGAA GGGGAACCTC CTGGCTCTAG CTGTGGAGGC AGCACGAGTC 
AGATGCACGG TTGGTGAAAT CACTGACGCA ATGAAGAAAG TGTTTGGTGA ACACAAGGCC 
AGCACCAGGA TGGTGAGTGG AGCTTACCGC AGCGAGTTTG GTGAGCATGA GGAGATCTCC 
GTGGCCCACG GCAGAGTTGC AGAATTTAAA AGCCAAGAGG GCAGGAACCC TCGGCTCCTG 
GTGGCAAAGA TGGGGCAGGA TGGCCATGAC AGAGGAGCTA AAGTCATCGC CACTGGCTTT 
GCAGACTTGG GCTTTGATGT GGACATTGGA CCACTGTTCC AGACTCCCGT TGAGGTGGCC 
CAGCAGGCCG TGGACGCAGA CGTCCACTGT GTTGGAGTCA GCACGCTCGC TGCCGGACAC 
AAGACCCTGG TCCCAGAGCT CATCAAGGAG CTGCGGAAGC TCAACAGGCC AGATATCCTC 
GTTATCTGCG GAGGAGTCAT CCCACCACAG GACTACGAGT TCCTGTATCA GAGTGGTGTG 
TGTGCCATCT TCGGCCCTGG AACCAGGATC CCACAAGCTG CCGTCGAAGT CATCGACAAC 
ATAGAGAAGA GTTTGGAGAA TATACGGCAA GCCATGTAG