ti cDNA ORF clone, Neurospora crassa OR74A

The following ti gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ti cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ONf09475 XM_001728356.2
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Neurospora crassa OR74A oligosaccharyl transferase STT3 subunit (ti), mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ONf09475
    Clone ID Related Accession (Same CDS sequence) XM_001728356.2
    Accession Version XM_001728356.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2250bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-10
    Organism Neurospora crassa OR74A
    Product oligosaccharyl transferase STT3 subunit
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026501). On Feb 23, 2015 this sequence version replaced XM_001728356.1.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGTCGGCCG ATTCCCTAGA ACGTCTCGCC AGCGCGGGCA AAGGGGATAG CACCCGCACA 
    TTGCTGCGAG TGATTATCCT CTTGCTTGTC GCCGGTGCCG CTATCTCAAG CCGCCTGTTT
    TCTGTTATTC GTTTCGAATC TATCATCCAT GAATTCGATC CTTGGTTCAA CTTCCGAGCG
    ACAAAATACT TGGTCGCAAA TGGATTCTAC AAGTTTTGGG ACTGGTTCGA TGACCGGACA
    TGGCATCCCC TGGGCCGCGT CACTGGAGGT ACTCTCTATC CCGGGCTCAT GGTCACCAGC
    GGTGTCATTT ACCACGCTCT GAGGGCTCTC TCGATTCCCG TCGACATCCG GAATGTCTGC
    GTTCTTCTTG CACCGGCCTT TTCCGGTCTT ACTGCCTACG CCGCTTACCT CCTCACCAAC
    GAGATGACCC CGTCTTCGTC CGCCGGTCTC CTTGCCGCCA TCTTCATGGG TATTGCCCCT
    GGCTATATCT CCCGTTCGGT CGCTGGCAGT TACGACAACG AAGCCATTGC CATTTTTCTG
    CTGGTCTTCA CCTTCTACCT GTGGATCAAG GCTCTCAAGC TCGGATCCAT GCTTTGGGGA
    GCTCTTTGCG CCCTCTTCTA CGGTTACATG GTTGCTTCCT GGGGTGGTTA TGCCTTCATC
    ACCTGTCTCC TCCCCCTCCA CGCATTTGTT CTTATCTGCA TGGGTCGCTA CAGCACTAGG
    CTTTATGTTG CGTACACCAC TTGGTATGCA TTGGGAACAT TGGCCAGCAT GCAGATTCCC
    TTTGTTGGTT TTCTGCCTGT CAAGACTAGC GAGCACATGC CTGCTTTGGG CATCTTTGGA
    TTCCTTCAGC TTTTGGCTTT CCTCGACTAC GTTCGTTCAG CGATTCCCAG CCGGCAATTC
    CAGACCTTCT TGTGGGTCTT TGCTGGAAGT ATCTTTGGTA TCGGCCTCAT CGGCCTTGTC
    GTTGCTACCA GTGCTGGTTT CATTGCTCCC TGGAGTGGTC GATTCTACTC CCTGTGGGAC
    ACGGGATACG CCAAGATTCA CATTCCCATC ATCGCCTCGG TCTCTGAGCA TCAGCCTACA
    GCATGGCCCG CCTTCTTCTT CGACCTCAAC ATGCTCGTGT GGCTCTTGCC CGCTGGCGTC
    TATCTGTGCT ACCAGAAGCT GGCTGATGAG CATGTCTTCG TCATTGTTTA CGCCTTGTTT
    GGCAGCTACT TCGCCGGTGT CATGGTTCGT CTCATGTTGA CGCTCACCCC TGTTGTTTGC
    GTTGCGGCGG CCATTGCGCT CTCCACCATC CTTCACACGT ATCTCAAGGC CAAGTCACCC
    ACCCCGGAGG AGCTTCTTCC TGAGGAATCT ACCGACGGTA CCTCCAAGAA AGCATCGAAG
    TCGGGTCTTA GGGCGTCGAA CAAGCCATAC ATCGGCATCT ATGCGACCTT CTCCAAGGTC
    ACCATTGTTG GTCTCATGAC CGCCTACCTA TTGCTGTTTG TTGCTCACTG CACATGGGTC
    ACCTCAAACG CTTATTCGTC ACCTTCAGTC GTCTTGGCCA GTAGGATGCC CGATGGTAGC
    CAGCACATCA TTGACGATTA TCGTGAGGCG TATCAGTGGT TGCGCCAGAA CACCAAGTAT
    GACGCCAAGA TCATGTCTTG GTGGGATTAT GGCTACCAGA TTGGTGGCAT GGCCGACCGT
    CCCACGCTTG TCGACAACAA CACCTGGAAC AACACACACA TCGCGACTGT CGGCAAGGCC
    ATGGCCTCCC GCGAGGAAGT CAGCTATCCC ATCATGCGCC AACACGAGGT GGACTATGTC
    TTGATTGTTT TCGGTGGCCT TTTGGGATAT TCCGGCGACG ATATTAACAA GTTCCTCTGG
    ATGGTTCGTA TTGCCGAGGG CATTTGGCCT GATGAGGTCA AGGAGCGCGA GTTCTTTACG
    CCTCGCGGTG AATATCGTGT CGATGCCGGG GCTACCGACA CCATGAAGAA CAGCTTGATG
    TACAAGATGT CGTATTATAA CTACAACAAC CTCTTTCCCC CTGGACAGGC TCAAGATCGC
    GTCCGTGGCG TCCGCCTTCC CGACGAAGGC CCGGTTCTCA ACACCATCGA GGAGGCCTTC
    ACGAGCGAAA ACTGGATTAT CCGCATTTAC AAGGTCAAGG ACCTCGATAA CCTAGGCCGT
    GACCATGCTT CTGCTTCTGC ATTTGAGCGC GGCCAGAAGA AGAAAAAGGT GATAAAGAAG
    AAGGGACCTC GGGTTCTGCG TGTAGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_001728408.1
    CDS291..2540
    Translation

    Target ORF information:

    RefSeq Version XM_001728356.2
    Organism Neurospora crassa OR74A
    Definition Neurospora crassa OR74A oligosaccharyl transferase STT3 subunit (ti), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_001728356.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGTCGGCCG ATTCCCTAGA ACGTCTCGCC AGCGCGGGCA AAGGGGATAG CACCCGCACA 
    TTGCTGCGAG TGATTATCCT CTTGCTTGTC GCCGGTGCCG CTATCTCAAG CCGCCTGTTT
    TCTGTTATTC GTTTCGAATC TATCATCCAT GAATTCGATC CTTGGTTCAA CTTCCGAGCG
    ACAAAATACT TGGTCGCAAA TGGATTCTAC AAGTTTTGGG ACTGGTTCGA TGACCGGACA
    TGGCATCCCC TGGGCCGCGT CACTGGAGGT ACTCTCTATC CCGGGCTCAT GGTCACCAGC
    GGTGTCATTT ACCACGCTCT GAGGGCTCTC TCGATTCCCG TCGACATCCG GAATGTCTGC
    GTTCTTCTTG CACCGGCCTT TTCCGGTCTT ACTGCCTACG CCGCTTACCT CCTCACCAAC
    GAGATGACCC CGTCTTCGTC CGCCGGTCTC CTTGCCGCCA TCTTCATGGG TATTGCCCCT
    GGCTATATCT CCCGTTCGGT CGCTGGCAGT TACGACAACG AAGCCATTGC CATTTTTCTG
    CTGGTCTTCA CCTTCTACCT GTGGATCAAG GCTCTCAAGC TCGGATCCAT GCTTTGGGGA
    GCTCTTTGCG CCCTCTTCTA CGGTTACATG GTTGCTTCCT GGGGTGGTTA TGCCTTCATC
    ACCTGTCTCC TCCCCCTCCA CGCATTTGTT CTTATCTGCA TGGGTCGCTA CAGCACTAGG
    CTTTATGTTG CGTACACCAC TTGGTATGCA TTGGGAACAT TGGCCAGCAT GCAGATTCCC
    TTTGTTGGTT TTCTGCCTGT CAAGACTAGC GAGCACATGC CTGCTTTGGG CATCTTTGGA
    TTCCTTCAGC TTTTGGCTTT CCTCGACTAC GTTCGTTCAG CGATTCCCAG CCGGCAATTC
    CAGACCTTCT TGTGGGTCTT TGCTGGAAGT ATCTTTGGTA TCGGCCTCAT CGGCCTTGTC
    GTTGCTACCA GTGCTGGTTT CATTGCTCCC TGGAGTGGTC GATTCTACTC CCTGTGGGAC
    ACGGGATACG CCAAGATTCA CATTCCCATC ATCGCCTCGG TCTCTGAGCA TCAGCCTACA
    GCATGGCCCG CCTTCTTCTT CGACCTCAAC ATGCTCGTGT GGCTCTTGCC CGCTGGCGTC
    TATCTGTGCT ACCAGAAGCT GGCTGATGAG CATGTCTTCG TCATTGTTTA CGCCTTGTTT
    GGCAGCTACT TCGCCGGTGT CATGGTTCGT CTCATGTTGA CGCTCACCCC TGTTGTTTGC
    GTTGCGGCGG CCATTGCGCT CTCCACCATC CTTCACACGT ATCTCAAGGC CAAGTCACCC
    ACCCCGGAGG AGCTTCTTCC TGAGGAATCT ACCGACGGTA CCTCCAAGAA AGCATCGAAG
    TCGGGTCTTA GGGCGTCGAA CAAGCCATAC ATCGGCATCT ATGCGACCTT CTCCAAGGTC
    ACCATTGTTG GTCTCATGAC CGCCTACCTA TTGCTGTTTG TTGCTCACTG CACATGGGTC
    ACCTCAAACG CTTATTCGTC ACCTTCAGTC GTCTTGGCCA GTAGGATGCC CGATGGTAGC
    CAGCACATCA TTGACGATTA TCGTGAGGCG TATCAGTGGT TGCGCCAGAA CACCAAGTAT
    GACGCCAAGA TCATGTCTTG GTGGGATTAT GGCTACCAGA TTGGTGGCAT GGCCGACCGT
    CCCACGCTTG TCGACAACAA CACCTGGAAC AACACACACA TCGCGACTGT CGGCAAGGCC
    ATGGCCTCCC GCGAGGAAGT CAGCTATCCC ATCATGCGCC AACACGAGGT GGACTATGTC
    TTGATTGTTT TCGGTGGCCT TTTGGGATAT TCCGGCGACG ATATTAACAA GTTCCTCTGG
    ATGGTTCGTA TTGCCGAGGG CATTTGGCCT GATGAGGTCA AGGAGCGCGA GTTCTTTACG
    CCTCGCGGTG AATATCGTGT CGATGCCGGG GCTACCGACA CCATGAAGAA CAGCTTGATG
    TACAAGATGT CGTATTATAA CTACAACAAC CTCTTTCCCC CTGGACAGGC TCAAGATCGC
    GTCCGTGGCG TCCGCCTTCC CGACGAAGGC CCGGTTCTCA ACACCATCGA GGAGGCCTTC
    ACGAGCGAAA ACTGGATTAT CCGCATTTAC AAGGTCAAGG ACCTCGATAA CCTAGGCCGT
    GACCATGCTT CTGCTTCTGC ATTTGAGCGC GGCCAGAAGA AGAAAAAGGT GATAAAGAAG
    AAGGGACCTC GGGTTCTGCG TGTAGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The genome sequence of the filamentous fungus Neurospora crassa.
    Nature422(6934)859-68(2003 Apr)
    Galagan JE,Calvo SE,Borkovich KA,Selker EU,Read ND,Jaffe D,FitzHugh W,Ma LJ,Smirnov S,Purcell S,Rehman B,Elkins T,Engels R,Wang S,Nielsen CB,Butler J,Endrizzi M,Qui D,Ianakiev P,Bell-Pedersen D,Nelson MA,Werner-Washburne M,Selitrennikoff CP,Kinsey JA,Braun EL,Zelter A,Schulte U,Kothe GO,Jedd G,Mewes W,Staben C,Marcotte E,Greenberg D,Roy A,Foley K,Naylor J,Stange-Thomann N,Barrett R,Gnerre S,Kamal M,Kamvysselis M,Mauceli E,Bielke C,Rudd S,Frishman D,Krystofova S,Rasmussen C,Metzenberg RL,Perkins DD,Kroken S,Cogoni C,Macino G,Catcheside D,Li W,Pratt RJ,Osmani SA,DeSouza CP,Glass L,Orbach MJ,Berglund JA,Voelker R,Yarden O,Plamann M,Seiler S,Dunlap J,Radford A,Aramayo R,Natvig DO,Alex LA,Mannhaupt G,Ebbole DJ,Freitag M,Paulsen I,Sachs MS,Lander ES,Nusbaum C,Birren B