tim50 cDNA ORF clone, Neurospora crassa OR74A

The following tim50 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the tim50 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ONf13249 XM_960532.3
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Neurospora crassa OR74A mitochondrial import inner membrane translocase subunit tim-50 (tim50), mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ONf13249
    Clone ID Related Accession (Same CDS sequence) XM_960532.3
    Accession Version XM_960532.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1623bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-10
    Organism Neurospora crassa OR74A
    Product mitochondrial import inner membrane translocase subunit tim-50
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026501). On Feb 23, 2015 this sequence version replaced XM_960532.2.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGATGCTGT CCAGAGCTGC CGTGCGCTCC ATCGCGGGCG CGCGTGTTGC AGCTCACGCG 
    GCGTCGCCTC TCCTGTCGCA GCGCACCCTG CCCGCCGTCT GGACCCGTTC CATGGCCAAG
    GACAACAAGC CTCCCAAGTT CTCCAAGCCC GAGAGCACTC CCGCACAAAA GGCTACTCCC
    AAGGCCCCCG AGCCCGCCGA ATCCGAGAAA GCTGAGCAGA AGCAGCAGCA ACAGCAGCAA
    CAACAAACCC CAGCCGAATC CGAACCCGAA CCCGAAATCG ACCTGTCCAA GCTCCCCGAT
    CTAAGAGGTG GTATTCCCAC CACCCTAGAG TATGAGATGG CCCAGAAGGA GGCCGGCAAG
    AAGCCGGTCG CCGGTGAGGA GGCAGAGACA CAGGCTGAGG GCGCCGAGGG CCCTGAGGCC
    GCTACGTCAG GCAGCGGTGG TGGCGGCCGC AAGAAGGGCC AGCTCCCCGA TTCTGCCTAC
    GTTTCTTCTA CTGAGAAGCG CCGCCAAAAG ATGGCCAACT GGGCCTTCAT TGCCGCTGGT
    CTCGCCTTGG TGGGTGGAAC GATCTACTTG GGAAGGGAAT GGGATGAGGA GGAGCTTGAG
    AAGCACCATG ATATACCAAA TGGCTGGGGT CTCGGCCTGT GGTGGAAGAG GGCCAAGGCC
    AGGATGACTG GAACCGTCAG CTACTACCAG GAACCTGCCT TCGAGAAGCT GCTACCCGAC
    CCGGATCCGT CATTCGAGCG TCCGTACACC CTCTGTATCA GTTTGGAAGA TATGCTGGTG
    CATAGCGAGT GGACGCGCGA CCACGGCTGG CGTCTCGCCA AGCGTCCTGG TGTCGACTAC
    TTCTTGCGCT ACCTCAGCCA GTACTACGAG ATTGTTCTCT TCACCAGCGT TCCCTTTGCC
    AACGCTGAGC CTATTGTCCG CAAGATGGAC CCGTACCGCT TCATCATGTG GCCCCTCTTT
    AGGGAGGCGA CCAAGTACAA GGATGGCGAG ATCGTCAAGG ACCTTTCTTA TCTCAACAGA
    GACTTGTCCA AGGTCATCAT CATTGATACC GACCCCAAGC ACGTGCGCGC CCAGCCCGAG
    AACGCCATCG TCCTCCCCAA GTGGAAGGGT GATCCCAAGG ATACCGAGCT CGTGTCCCTC
    GTCCCCTTCC TCGAGTTCAT CCACACCATG AACTTCCCCG ATGTTCGCAA GGTCCTCAAG
    TCCTTCGAGG GCCAGCACAT CCCCACCGAG TTTGCCCGTA GAGAGGCCAT TGCCCGCGCT
    GAGCACAACA AGCTCGTTGC CGCCAAGGCC AAGAAGGCTG GCTTGGGCTC GCTTGGCGCC
    CGCTTCGGCA TCAAGCCTAG CAAGCTGAAC CCCATGGCCA TGGAGGGCGA GGAGGATCCT
    TCTGAGGCTT TTGCTAAGGG CAAGATGATC CAGGATATTG CCCGTGAGCG CGGCATGCGC
    AACTACCTGG CCATGGAGGA GGAGATCAAG AAGAACGGCG AGATGTGGCT CAAGATGGAG
    CAGGAGGCCC AGGAGAAGGC CCAGAAAGAG ATGATGAAGA ACATGCAGAG TTCCGTCTTT
    GGCTGGTTCG GCGGTGCTCC CAGCGGCGAG CAGCAGTCCG GCGAGTCAGA GAAGAAGGCC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_965625.3
    CDS272..1894
    Translation

    Target ORF information:

    RefSeq Version XM_960532.3
    Organism Neurospora crassa OR74A
    Definition Neurospora crassa OR74A mitochondrial import inner membrane translocase subunit tim-50 (tim50), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_960532.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGATGCTGT CCAGAGCTGC CGTGCGCTCC ATCGCGGGCG CGCGTGTTGC AGCTCACGCG 
    GCGTCGCCTC TCCTGTCGCA GCGCACCCTG CCCGCCGTCT GGACCCGTTC CATGGCCAAG
    GACAACAAGC CTCCCAAGTT CTCCAAGCCC GAGAGCACTC CCGCACAAAA GGCTACTCCC
    AAGGCCCCCG AGCCCGCCGA ATCCGAGAAA GCTGAGCAGA AGCAGCAGCA ACAGCAGCAA
    CAACAAACCC CAGCCGAATC CGAACCCGAA CCCGAAATCG ACCTGTCCAA GCTCCCCGAT
    CTAAGAGGTG GTATTCCCAC CACCCTAGAG TATGAGATGG CCCAGAAGGA GGCCGGCAAG
    AAGCCGGTCG CCGGTGAGGA GGCAGAGACA CAGGCTGAGG GCGCCGAGGG CCCTGAGGCC
    GCTACGTCAG GCAGCGGTGG TGGCGGCCGC AAGAAGGGCC AGCTCCCCGA TTCTGCCTAC
    GTTTCTTCTA CTGAGAAGCG CCGCCAAAAG ATGGCCAACT GGGCCTTCAT TGCCGCTGGT
    CTCGCCTTGG TGGGTGGAAC GATCTACTTG GGAAGGGAAT GGGATGAGGA GGAGCTTGAG
    AAGCACCATG ATATACCAAA TGGCTGGGGT CTCGGCCTGT GGTGGAAGAG GGCCAAGGCC
    AGGATGACTG GAACCGTCAG CTACTACCAG GAACCTGCCT TCGAGAAGCT GCTACCCGAC
    CCGGATCCGT CATTCGAGCG TCCGTACACC CTCTGTATCA GTTTGGAAGA TATGCTGGTG
    CATAGCGAGT GGACGCGCGA CCACGGCTGG CGTCTCGCCA AGCGTCCTGG TGTCGACTAC
    TTCTTGCGCT ACCTCAGCCA GTACTACGAG ATTGTTCTCT TCACCAGCGT TCCCTTTGCC
    AACGCTGAGC CTATTGTCCG CAAGATGGAC CCGTACCGCT TCATCATGTG GCCCCTCTTT
    AGGGAGGCGA CCAAGTACAA GGATGGCGAG ATCGTCAAGG ACCTTTCTTA TCTCAACAGA
    GACTTGTCCA AGGTCATCAT CATTGATACC GACCCCAAGC ACGTGCGCGC CCAGCCCGAG
    AACGCCATCG TCCTCCCCAA GTGGAAGGGT GATCCCAAGG ATACCGAGCT CGTGTCCCTC
    GTCCCCTTCC TCGAGTTCAT CCACACCATG AACTTCCCCG ATGTTCGCAA GGTCCTCAAG
    TCCTTCGAGG GCCAGCACAT CCCCACCGAG TTTGCCCGTA GAGAGGCCAT TGCCCGCGCT
    GAGCACAACA AGCTCGTTGC CGCCAAGGCC AAGAAGGCTG GCTTGGGCTC GCTTGGCGCC
    CGCTTCGGCA TCAAGCCTAG CAAGCTGAAC CCCATGGCCA TGGAGGGCGA GGAGGATCCT
    TCTGAGGCTT TTGCTAAGGG CAAGATGATC CAGGATATTG CCCGTGAGCG CGGCATGCGC
    AACTACCTGG CCATGGAGGA GGAGATCAAG AAGAACGGCG AGATGTGGCT CAAGATGGAG
    CAGGAGGCCC AGGAGAAGGC CCAGAAAGAG ATGATGAAGA ACATGCAGAG TTCCGTCTTT
    GGCTGGTTCG GCGGTGCTCC CAGCGGCGAG CAGCAGTCCG GCGAGTCAGA GAAGAAGGCC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The genome sequence of the filamentous fungus Neurospora crassa.
    Nature422(6934)859-68(2003 Apr)
    Galagan JE,Calvo SE,Borkovich KA,Selker EU,Read ND,Jaffe D,FitzHugh W,Ma LJ,Smirnov S,Purcell S,Rehman B,Elkins T,Engels R,Wang S,Nielsen CB,Butler J,Endrizzi M,Qui D,Ianakiev P,Bell-Pedersen D,Nelson MA,Werner-Washburne M,Selitrennikoff CP,Kinsey JA,Braun EL,Zelter A,Schulte U,Kothe GO,Jedd G,Mewes W,Staben C,Marcotte E,Greenberg D,Roy A,Foley K,Naylor J,Stange-Thomann N,Barrett R,Gnerre S,Kamal M,Kamvysselis M,Mauceli E,Bielke C,Rudd S,Frishman D,Krystofova S,Rasmussen C,Metzenberg RL,Perkins DD,Kroken S,Cogoni C,Macino G,Catcheside D,Li W,Pratt RJ,Osmani SA,DeSouza CP,Glass L,Orbach MJ,Berglund JA,Voelker R,Yarden O,Plamann M,Seiler S,Dunlap J,Radford A,Aramayo R,Natvig DO,Alex LA,Mannhaupt G,Ebbole DJ,Freitag M,Paulsen I,Sachs MS,Lander ES,Nusbaum C,Birren B