ORF » Species Summary » Neurospora crassa OR74A » lys-3 cDNA ORF clone

lys-3 cDNA ORF clone, Neurospora crassa OR74A

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  • gRNAs

The following lys-3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the lys-3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ONf08658 XM_960303.2
Latest version!		 Neurospora crassa OR74A L-aminoadipate-semialdehyde dehydrogenase large subunit (lys-3), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    lys-3 ( XM_960303.2 ) cDNA ORF clone, Neurospora crassa OR74A -> XP_965396.1 Neurospora crassa OR74A L-aminoadipate-semialdehyde dehydrogenase large subunit (lys-3), mRNA.
    Price & Availability↑
    CloneID ONf08658
    Clone ID Related Accession (Same CDS sequence) XM_960303.2
    Accession Version XM_960303.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3525bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-10
    Organism Neurospora crassa OR74A
    Product L-aminoadipate-semialdehyde dehydrogenase large subunit
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026501). On Feb 23, 2015 this sequence version replaced XM_960303.1. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCGTCTG TATTACCCGA TCCCACAATC GACCTCGACT GGTCCGGGTA TGTCGGTTCC 
    ATCCAGGAGC ACTTCAGACG TAACGCCGAG GCACACCCCC AAAGGACATG CGTCGTCGAG 
    ACCAAGACTT CCTCAACACC AGAGAGACGT TTCACATACC GCCAGATCTA TGAAGCCTCC 
    AACACGCTAG CCTGGTATCT GCACAAGGCC GGCATCACCA ATGGCGATGT CGTCATGATT 
    TGGGCCCATC GGTCCGTCGA CTTGGTGGTG GCCCTTATGG GTATTCTGGC TTCTGGCGCG 
    ACCATGACAG TTCTCGATCC TGCCTATCCT CCGGCAAGAC AGCAGATCTA CCTCGAGGTC 
    TCCCAGCCCA ATGCGCTCCT ACGAATTGGC CGTGCCACAG ACGAGAACGG CCCATTGGCC 
    CCCTTGGTCC AGAAGTACAT TGACGACGAG CTCAAGCTCA AGACCGACGT ACCAGATCTG 
    CGCCTCCGCG ACGACGGTTT CCTCTACGGT GGTGAGGTCG ATGGCAAGGA CATCTTTGCC 
    GAAGTAAGGC AATTGGCATC CGCGCCTCCC GATGTCATCG TCGGTCCCGA CTCGAACCCC 
    ACACTATCCT TCACTTCCGG AAGTGAGGGT CGCCCTAAGG GTGTCCTCGG TCGTCACTAT 
    AGTCTGGTCA AGTATTTCGG CTGGATGGCC GAGCGCTTCA ACCTCTCGTC CGAGAGCAGA 
    TACACCCTCC TTTCCGGCAT TGCCCACGAC CCAGTACAGC GCGATATCTT CACCCCTCTC 
    TTCCTTGGCG CCCAGCTTCT TGTTCCATCC CGTGAGGATA TCCAGCATGA GAAGCTTGCC 
    GAGTGGATGA GGGAGCACAA GCCCACTGTC ACCCATCTGA CTCCCGCTAT GGGTCAGATT 
    CTTGTGGGCG GCGCTTCCGC TGAGTTCCCC AGCCTGGAGC ATGTCTTCTT CGTCGGCGAC 
    GTTTTGACCA CCCGTGATTG CCGCGCCTTG AGACGCTTGG CTGAGAATGC CAACATCATC 
    AACATGTACG GCACTACCGA GACCCAGCGT GCCGTCAGTT ACTTCGAGAT CCCCAGCCGC 
    AACAGGGACC CTGACTTCCT CGAAAGAGAG CTTAAGGACA CCGTCCCTGC GGGCACGGGC 
    ATGCAAAACG TCCAGCTCCT GGTCGTCAAC CGGGAGAACC GTGAGCAGCA GTGCCAGGTT 
    GGCGAGGTTG GAGAGATATA CGTTCGTGCT GCCGGTCTTG CCGAAGGATA CCTCGGCGAC 
    CCGGCTCTCA ACGAGCAGAA GTTCCTGAAC AACTGGTTCG TCGACAACAA CAAGTGGGTT 
    GAGGCGGATG CCAAGCTTAA CAAGAACGAG CCATGGAGAA AGTACTACAA GGGTCCAAGA 
    GACAGACTCT ACCGCACTGG CGATCTTGGA CGCTACCTTG AGTCTGGAGA TGTCGAATGT 
    GTCGGCCGCG CTGATGATCA GGTCAAGATC CGTGGTTTCC GTATCGAGCT CAACGACATT 
    GATAGCAACT TGAGCCAAAG CCCTCTTATC CGTGACTGCA AGACTCTTGT GCGGAGAGAT 
    CGTAACGAGG AGGCGACTCT TGTCAGCTAC ATCGTCCCCG AGCACAAGGA ATGGCTCAAG 
    TGGCTCGAGG TCCGTGGCCT CGCCGACGTC GAGGACGAGG GTGTCGAGAT GGGTCCCGTA 
    ACCGTTTACC TCAAGAAGTA CAGGCGCATG CAGGCCGAGA TCCGCGATCA CCTGGGCGCT 
    CGTCTGCCTA CCTACGCTGT CCCCACCATT TATGTCGTCC TCAAGAAGAT GCCTCTGAAC 
    CCCAACGGCA AGGTTGACAA GCCGAACCTT CCTTTCCCCG ATGTGGCTGA GCGTGTTGAG 
    GATGCCTCCG AGGAGGACCT CAAGAACTGG GAATCGCTTA CCGAGACCGA GCGCACCGTG 
    GCCCAGCTCT GGGCTGATGT GATTCTCGGC CTTAACCCCC AGACCATCAA GCGTGAGAAC 
    TCTTTCTTCG ATCTGGGCGG TCACAGTTTG TTGGCTCAAC AGTTCCTCCT GAACATTCGT 
    AAGGCGATTG GCAGGGATGT GTCTATCAAC ATTCTCTATG AGCACCCTAG CCTCGCTGGG 
    TTCTGTGCCC AAGTCGACAA GCTCCTCGGA GAGGAGACCA GCGGCGTCAC TGCCGAGGCT 
    GGTCAGGATG AGTACGCCAA GTCTTTGGAC GAGCTTTTGG CTCAGCTTCC TGAGAAGTAT 
    CTCTCGGCCG ATCCCGCGGC TCTTGGCTCT GAGGAGTTGA CCGTCTTCCT CACTGGTGCG 
    ACTGGATTCC TCGGCTCCTA CCTGGTCAAG GACGTCCTTG ACCGCACAGC CCGCACGGTC 
    AAGCTGATTG CCCACGTCCG TGGTGTCAAG GACTCTACGG CGGCGCTCGA GCGTCTCCGT 
    CGCTCTCTCC AGGGTTACGG CCTCTGGAAT GATGAGTGGA CTGGAAGACT CAGCACCGTC 
    GTCGGTGACT TGGCGAAGCC CAACCTTGGC ATCGATGATG CCGATTGGGC GGATCTTGCT 
    CAGAAGGTGG ATGTTGTTAT TCACAACGGC GCCACTGTTC ACTGGGTCAA GCGCTATCAT 
    GACATGATGG CCGCCAACGT CTTGTCCACC GTTGACGCCA TGAAGCTTTG CAACGAGGGC 
    AAGCCCAAGG CCTTCTGCTT CGTCAGCTCC ACCAGTGTCC TCGACACGGA CTACTACTTC 
    GACCTGTCTG ACAAGCAGAC GAGCACCGGA CGGGGTGCCA TCATGGAGTC CGACGATATG 
    GAGGGCAGCC GCACTGGTCT CGGAACTGGC TACGGTCAGA CCAAGTGGGT CTCTGAGCAG 
    CTTGTCCGCG AAGCCGGCAA GCGTGGTCTC CTGGGTTCCG TTGTCCGCCC TGGCTACATC 
    TTGGGTGATG CCAACACCGG TGTCTGCAAT GTCGATGATT TCTTGGTCCG CATGCTCAAG 
    GGCTGCATCC AGATTTCCTC GCGTCCCCAC ATTGTCAACA CTGTCAACGC CGTTCCCGTC 
    AACCACGTCG CCCGCACCGT TGTGGCTGCC GCCCTCAACC CCATTCCCAG CGGCGTGCAC 
    GTCATCCACG TCACCGCCCA TCCTCGTCTC CGCATGAACG AGTACCTCGC CATCTTGGAG 
    TACTACGGCT ACAAGACTCC CGAGACTAGC TACGAGGAGT GGAAGGCCGA GCTGGAAAAG 
    TTCGTTTCTG CTGGTTCCTT GGTGAAGGAT CAGGAGCAGC ACGCTCTCAT GCCTCTTTAC 
    CACTTCTGCA TGAACGACCT TCCCGCCAAC ACTCGCGCCC CTGAGATGGA CGACAGGAAC 
    GCCGTGGCTG TTCTGAAGGC TGATGCTGAC AAGTGGACTG GCGTTGATGA CTCGACTGGT 
    AGCGGTATCG GCAGGGAGGA GGTTGGCAAG TTCCTTGCTT ACCTCTCTGA GATCAACTAT 
    GTTCCTAAGC CCACCGAGAG AGGTCGTCCT CTGCCCCAGA TGCAGCCTGA GGTTCTCAAG 
    GCTTTGGCCA TTGGCGCTAC TGGTGGTCGT GGTGGTGCTC ACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_965396.1
    CDS135..3659
    Translation

    Target ORF information:

    RefSeq Version XM_960303.2
    Organism Neurospora crassa OR74A
    Definition Neurospora crassa OR74A L-aminoadipate-semialdehyde dehydrogenase large subunit (lys-3), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_960303.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCGTCTG TATTACCCGA TCCCACAATC GACCTCGACT GGTCCGGGTA TGTCGGTTCC 
    ATCCAGGAGC ACTTCAGACG TAACGCCGAG GCACACCCCC AAAGGACATG CGTCGTCGAG 
    ACCAAGACTT CCTCAACACC AGAGAGACGT TTCACATACC GCCAGATCTA TGAAGCCTCC 
    AACACGCTAG CCTGGTATCT GCACAAGGCC GGCATCACCA ATGGCGATGT CGTCATGATT 
    TGGGCCCATC GGTCCGTCGA CTTGGTGGTG GCCCTTATGG GTATTCTGGC TTCTGGCGCG 
    ACCATGACAG TTCTCGATCC TGCCTATCCT CCGGCAAGAC AGCAGATCTA CCTCGAGGTC 
    TCCCAGCCCA ATGCGCTCCT ACGAATTGGC CGTGCCACAG ACGAGAACGG CCCATTGGCC 
    CCCTTGGTCC AGAAGTACAT TGACGACGAG CTCAAGCTCA AGACCGACGT ACCAGATCTG 
    CGCCTCCGCG ACGACGGTTT CCTCTACGGT GGTGAGGTCG ATGGCAAGGA CATCTTTGCC 
    GAAGTAAGGC AATTGGCATC CGCGCCTCCC GATGTCATCG TCGGTCCCGA CTCGAACCCC 
    ACACTATCCT TCACTTCCGG AAGTGAGGGT CGCCCTAAGG GTGTCCTCGG TCGTCACTAT 
    AGTCTGGTCA AGTATTTCGG CTGGATGGCC GAGCGCTTCA ACCTCTCGTC CGAGAGCAGA 
    TACACCCTCC TTTCCGGCAT TGCCCACGAC CCAGTACAGC GCGATATCTT CACCCCTCTC 
    TTCCTTGGCG CCCAGCTTCT TGTTCCATCC CGTGAGGATA TCCAGCATGA GAAGCTTGCC 
    GAGTGGATGA GGGAGCACAA GCCCACTGTC ACCCATCTGA CTCCCGCTAT GGGTCAGATT 
    CTTGTGGGCG GCGCTTCCGC TGAGTTCCCC AGCCTGGAGC ATGTCTTCTT CGTCGGCGAC 
    GTTTTGACCA CCCGTGATTG CCGCGCCTTG AGACGCTTGG CTGAGAATGC CAACATCATC 
    AACATGTACG GCACTACCGA GACCCAGCGT GCCGTCAGTT ACTTCGAGAT CCCCAGCCGC 
    AACAGGGACC CTGACTTCCT CGAAAGAGAG CTTAAGGACA CCGTCCCTGC GGGCACGGGC 
    ATGCAAAACG TCCAGCTCCT GGTCGTCAAC CGGGAGAACC GTGAGCAGCA GTGCCAGGTT 
    GGCGAGGTTG GAGAGATATA CGTTCGTGCT GCCGGTCTTG CCGAAGGATA CCTCGGCGAC 
    CCGGCTCTCA ACGAGCAGAA GTTCCTGAAC AACTGGTTCG TCGACAACAA CAAGTGGGTT 
    GAGGCGGATG CCAAGCTTAA CAAGAACGAG CCATGGAGAA AGTACTACAA GGGTCCAAGA 
    GACAGACTCT ACCGCACTGG CGATCTTGGA CGCTACCTTG AGTCTGGAGA TGTCGAATGT 
    GTCGGCCGCG CTGATGATCA GGTCAAGATC CGTGGTTTCC GTATCGAGCT CAACGACATT 
    GATAGCAACT TGAGCCAAAG CCCTCTTATC CGTGACTGCA AGACTCTTGT GCGGAGAGAT 
    CGTAACGAGG AGGCGACTCT TGTCAGCTAC ATCGTCCCCG AGCACAAGGA ATGGCTCAAG 
    TGGCTCGAGG TCCGTGGCCT CGCCGACGTC GAGGACGAGG GTGTCGAGAT GGGTCCCGTA 
    ACCGTTTACC TCAAGAAGTA CAGGCGCATG CAGGCCGAGA TCCGCGATCA CCTGGGCGCT 
    CGTCTGCCTA CCTACGCTGT CCCCACCATT TATGTCGTCC TCAAGAAGAT GCCTCTGAAC 
    CCCAACGGCA AGGTTGACAA GCCGAACCTT CCTTTCCCCG ATGTGGCTGA GCGTGTTGAG 
    GATGCCTCCG AGGAGGACCT CAAGAACTGG GAATCGCTTA CCGAGACCGA GCGCACCGTG 
    GCCCAGCTCT GGGCTGATGT GATTCTCGGC CTTAACCCCC AGACCATCAA GCGTGAGAAC 
    TCTTTCTTCG ATCTGGGCGG TCACAGTTTG TTGGCTCAAC AGTTCCTCCT GAACATTCGT 
    AAGGCGATTG GCAGGGATGT GTCTATCAAC ATTCTCTATG AGCACCCTAG CCTCGCTGGG 
    TTCTGTGCCC AAGTCGACAA GCTCCTCGGA GAGGAGACCA GCGGCGTCAC TGCCGAGGCT 
    GGTCAGGATG AGTACGCCAA GTCTTTGGAC GAGCTTTTGG CTCAGCTTCC TGAGAAGTAT 
    CTCTCGGCCG ATCCCGCGGC TCTTGGCTCT GAGGAGTTGA CCGTCTTCCT CACTGGTGCG 
    ACTGGATTCC TCGGCTCCTA CCTGGTCAAG GACGTCCTTG ACCGCACAGC CCGCACGGTC 
    AAGCTGATTG CCCACGTCCG TGGTGTCAAG GACTCTACGG CGGCGCTCGA GCGTCTCCGT 
    CGCTCTCTCC AGGGTTACGG CCTCTGGAAT GATGAGTGGA CTGGAAGACT CAGCACCGTC 
    GTCGGTGACT TGGCGAAGCC CAACCTTGGC ATCGATGATG CCGATTGGGC GGATCTTGCT 
    CAGAAGGTGG ATGTTGTTAT TCACAACGGC GCCACTGTTC ACTGGGTCAA GCGCTATCAT 
    GACATGATGG CCGCCAACGT CTTGTCCACC GTTGACGCCA TGAAGCTTTG CAACGAGGGC 
    AAGCCCAAGG CCTTCTGCTT CGTCAGCTCC ACCAGTGTCC TCGACACGGA CTACTACTTC 
    GACCTGTCTG ACAAGCAGAC GAGCACCGGA CGGGGTGCCA TCATGGAGTC CGACGATATG 
    GAGGGCAGCC GCACTGGTCT CGGAACTGGC TACGGTCAGA CCAAGTGGGT CTCTGAGCAG 
    CTTGTCCGCG AAGCCGGCAA GCGTGGTCTC CTGGGTTCCG TTGTCCGCCC TGGCTACATC 
    TTGGGTGATG CCAACACCGG TGTCTGCAAT GTCGATGATT TCTTGGTCCG CATGCTCAAG 
    GGCTGCATCC AGATTTCCTC GCGTCCCCAC ATTGTCAACA CTGTCAACGC CGTTCCCGTC 
    AACCACGTCG CCCGCACCGT TGTGGCTGCC GCCCTCAACC CCATTCCCAG CGGCGTGCAC 
    GTCATCCACG TCACCGCCCA TCCTCGTCTC CGCATGAACG AGTACCTCGC CATCTTGGAG 
    TACTACGGCT ACAAGACTCC CGAGACTAGC TACGAGGAGT GGAAGGCCGA GCTGGAAAAG 
    TTCGTTTCTG CTGGTTCCTT GGTGAAGGAT CAGGAGCAGC ACGCTCTCAT GCCTCTTTAC 
    CACTTCTGCA TGAACGACCT TCCCGCCAAC ACTCGCGCCC CTGAGATGGA CGACAGGAAC 
    GCCGTGGCTG TTCTGAAGGC TGATGCTGAC AAGTGGACTG GCGTTGATGA CTCGACTGGT 
    AGCGGTATCG GCAGGGAGGA GGTTGGCAAG TTCCTTGCTT ACCTCTCTGA GATCAACTAT 
    GTTCCTAAGC CCACCGAGAG AGGTCGTCCT CTGCCCCAGA TGCAGCCTGA GGTTCTCAAG 
    GCTTTGGCCA TTGGCGCTAC TGGTGGTCGT GGTGGTGCTC ACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The genome sequence of the filamentous fungus Neurospora crassa.
    Nature422(6934)859-68(2003 Apr)
    Galagan JE,Calvo SE,Borkovich KA,Selker EU,Read ND,Jaffe D,FitzHugh W,Ma LJ,Smirnov S,Purcell S,Rehman B,Elkins T,Engels R,Wang S,Nielsen CB,Butler J,Endrizzi M,Qui D,Ianakiev P,Bell-Pedersen D,Nelson MA,Werner-Washburne M,Selitrennikoff CP,Kinsey JA,Braun EL,Zelter A,Schulte U,Kothe GO,Jedd G,Mewes W,Staben C,Marcotte E,Greenberg D,Roy A,Foley K,Naylor J,Stange-Thomann N,Barrett R,Gnerre S,Kamal M,Kamvysselis M,Mauceli E,Bielke C,Rudd S,Frishman D,Krystofova S,Rasmussen C,Metzenberg RL,Perkins DD,Kroken S,Cogoni C,Macino G,Catcheside D,Li W,Pratt RJ,Osmani SA,DeSouza CP,Glass L,Orbach MJ,Berglund JA,Voelker R,Yarden O,Plamann M,Seiler S,Dunlap J,Radford A,Aramayo R,Natvig DO,Alex LA,Mannhaupt G,Ebbole DJ,Freitag M,Paulsen I,Sachs MS,Lander ES,Nusbaum C,Birren B


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