ubr1 cDNA ORF clone, Neurospora crassa OR74A

Gene
Clones
gRNAs

Summary

Gene Symbol	ubr1
Entrez Gene ID	3881483
Full Name	ubr1-like
Gene Type	protein-coding
Organism	Neurospora crassa OR74A

mRNA and Protein(s)

mRNA	Protein	Name
XM_960241.3	XP_965334.3	ubiquitin-protein ligase E3 component

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	UBR1	XP_510341.3
	UBR1	XP_001105721.2
Canis lupus familiaris (dog)	UBR1	XP_544645.2
Bos taurus (cattle)	UBR1	NP_001192826.1
Danio rerio (zebrafish)	ubr1	XP_002665264.3
Xenopus tropicalis (tropical clawed frog)	ubr1	XP_002941132.2
Rattus norvegicus (Norway rat)	Ubr1	NP_001171543.1
Homo sapiens (human)	UBR1	NP_777576.1
Mus musculus (house mouse)	Ubr1	NP_033487.2
Gallus gallus (chicken)	UBR1	XP_421165.3
Schizosaccharomyces pombe (fission yeast)	ubr1	NP_596158.2
Magnaporthe oryzae (rice blast fungus)	MGG_13171	XP_003710667.1
Neurospora crassa	NCU03234	XP_965334.2

Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Galagan JE, Calvo SE, Borkovich KA, Selker EU, Read ND, Jaffe D, FitzHugh W, Ma LJ, Smirnov S, Purcell S, Rehman B, Elkins T, Engels R, Wang S, Nielsen CB, Butler J, Endrizzi M, Qui D, Ianakiev P, Bell-Pedersen D, Nelson MA, Werner-Washburne M, Selitrennikoff CP, Kinsey JA, Braun EL, Zelter A, Schulte U, Kothe GO, Jedd G, Mewes W, Staben C, Marcotte E, Greenberg D, Roy A, Foley K, Naylor J, Stange-Thomann N, Barrett R, Gnerre S, Kamal M, Kamvysselis M, Mauceli E, Bielke C, Rudd S, Frishman D, Krystofova S, Rasmussen C, Metzenberg RL, Perkins DD, Kroken S, Cogoni C, Macino G, Catcheside D, Li W, Pratt RJ, Osmani SA, DeSouza CP, Glass L, Orbach MJ, Berglund JA, Voelker R, Yarden O, Plamann M, Seiler S, Dunlap J, Radford A, Aramayo R, Natvig DO, Alex LA, Mannhaupt G, Ebbole DJ, Freitag M, Paulsen I, Sachs MS, Lander ES, Nusbaum C, Birren B
Nature422(6934)859-68(2003 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following ubr1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ubr1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ONf13154	XM_960241.3 Latest version!	Neurospora crassa OR74A ubiquitin-protein ligase E3 component (ubr1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ubr1 ( XM_960241.3 ) cDNA ORF clone, Neurospora crassa OR74A -> XP_965334.3 Neurospora crassa OR74A ubiquitin-protein ligase E3 component (ubr1), mRNA.

Price & Availability↑

CloneID	ONf13154
Clone ID Related Accession (Same CDS sequence)	XM_960241.3
Accession Version	XM_960241.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6471bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-10
Organism	Neurospora crassa OR74A
Product	ubiquitin-protein ligase E3 component
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026501). On Feb 23, 2015 this sequence version replaced XM_960241.2.

ATGGAAGCCC AGATGTCGAC ACAGGAGCAG CAGCTCTGCC AGGCCTTGCT GGCGATGCCT 
GGCAAATACC GATACCGCTA CAGCGAAGAG GCATCGCGGG AACTTCTTCT CACCCTGTTC 
TGGTCCATGG CTGGTGGGAA AAACGAATAC ATGAAGCTCT TCTTTCCCCA AGGGGATCCA 
CGAAACACTT CCCTCAAGCT CAGGGATGCC CAAGGCGCGG TCGAGGGCGC CGAATACACC 
GAAGCCGCGC GTGGCAAGGC CTGCGGTCAC ATATTCAAAC AAGGAGAAGC ATCGTATGCG 
TGCAAGACCT GTAGCGCAGA CGACACCTGC TGCCTGTGCA GCAAGTGCTT CGAAGCCACC 
GACCATACAG GCCATATGGT GCGAATCAGT ATCTCGCCCG GAAACAGCGG ATGTTGCGAT 
TGCGGAGATT TGGAAGCGTG GAAGCGGCCC ATGTTTTGCA CAATACATTC GATGTGGGAG 
GGCGACCGCG ATAAGGGCAA GGGAAAAGCG ACAGAACTTC CAGAGGATCT GGTCAGGAAT 
ATCAGGATGA CCATCGGCCG CGTCTTCGAC TACATGTGCG ACGTCATCTC TTGCGCCCCC 
GAGCAGCTCC GCCAAACCAA AACCGTTCAG TCTATCCAAG AGGATGAAAG GCTCTCGAGG 
TTATCATCGA CATACTGCGG CGGAGAGACT GACTCGCCGG GGGAATATGC CGTTCTGCTG 
TGGAATGATG AAAAGCACAC CGTGGTCGAT GTGCGTGACC AAGTGGCTCG GGCGTGCAAC 
ACCACGCTTG CAAATGGCTT GAAAGCTGCT TACGAGACCG ATGCCATTGG TAGGAGTATC 
CTGATGTACG ACAGCAGTCT GGAAAAGCTG CTCGAAGTAG CCGAGGTACT TGAGAGGATA 
AGGGTTACCG TTACCATTCG CTCTTCCAGG GATACCTTCA GAGAGCAAAT GTGCGGCACC 
ATAATTGAGT GGCTTAGAGA TATCTCTGGC TGCCGAGTTG GCCACGACCA TCAGCTTTTG 
AGAAACATCG TGTGCGAGGA AATGCTTAAG CCTTGGAGGA AAGGTAGCCC CGCGGCACAT 
GCCATTGTCG GCAAAGATGG CATTGACGAT GAAGACCGTA TTGACCAAGA AGAGAACGTA 
CCAAGCGGGT TCTACGAGCA GCAAGGCAGG CTTTTGCTTC AGGCACGTCA AGCTGCCCGT 
AGGATGGCAG AGGCTCGTGA GGCTGGCGAA GATGAAGGGG ATGAAGACGA TGAAGACGGG 
GACGACATCA TTGTCTCTGG TGGAGAAATG GATGAAGATG AGGAGGGGGA CGAAGATGTC 
ATGATGCTTG ACGAACAAGG TGACGCAGCT GGGGATGATG TTGCCATGAC GGACTGGCGA 
GAAGTTAGGG TGGTGCAGGT AGCCCCGGCC GCTGCTGGCG GCCGTCCTCT TCCGCCACCT 
CCCCCTCCGG CACCGACTAC TGCTACGGCT CACCGACGAG CCATTCGGGA ACGCGAGCAC 
ACTCCTGATG ATTCCGATAC CGCCGAGCCA CTCATCGCGC CAAATATCTA CACCAAGGCC 
CACCTCGACA TTCCAAAGAC CCCAGGCAGT CCGAAAGCCA AGACATCGCC TCCAAATCCT 
GGACGTTACT GGTTGCAGAC TCCTGCCGGC TACGTGGAGG AAAGTAACAT TCCGGTTGCC 
GAAGACCTTT TCGAGCGAGT TCGTCTAGAC TGGCTGATAC TATTTGACTT GCGGATGTGG 
AAGAAGGTTC GCTGTGATCT TCGGTCATTA TACATCTCAA CCGTCGTTAC GATCCCCGAG 
TTCAAACGTG TACTCGGCTT GCGGTTTGCT GGCCTTTACA CCACTCTGGC TCAGTTGTAC 
CTCATCGGCG ATCGTGAGCC TGATCACTCC ATCATCAACA TCTCCTTACA GATGCTGACA 
ACTCCCTCCA TTACTGCTGA AATCGTTGAG CGGGGAAATT TCCTTACCAG TCTGATGGCC 
ATTCTTTACA CATTTTTGAC CACTCGTCAA GTCGGACACC CTTGGGACGT GGCAAGCAAC 
GCAGTTTTGT CCTTCGATTC AGGCTCGGTT ACGAACCGGC GCATGTATCA TTTCTTCTTA 
GATCTCAAGT ATCTCTTTGG GTCTCCGCAC GTTCAGGAAC GCCTCCGGAC AGAGGAGCGA 
TATATGATGC AGTTCCTCGA CCTCGTCAAG TTGCATCAAG GTATCTGCCC GAACGTCAGG 
GCTGTCGGCG AACACGTCGA ATATGAGACA GATAGCTGGA TCAGCGCGTC TCTTGTCACA 
CGGGAGATCA ACAGACTTTG CAGACAGTTC GCCCAATCCT TCCGGAACGT CAAGGAACAC 
GGTTTGCAGT ATGTCAACAA TGCAATCAGA CTGGCTGCAA AGGTTACCAT TATCAACTCA 
CTTGGTGCCG AGCGTCACCG GTTTAACCAG TCAGAAATCA AGGAAGAGAT TTCCTTCAGA 
AACCTTACAG ATTTTGAGTT TGAGCCAACT GGGCGAAGTT TCAAGGTCGT CCACTTTGTG 
GTAGAGGAGC AACCCAACAG TTTTCATCAT GCCCTTCACT ACACCTTGTC GTGGCTTATC 
GAGTGCGGCA AATCGATGAG CGTTGACGAC CTGCGCAAGC TCCTTACTTT CGAAACCTGC 
GATTTGCTAG CAAAACCTCA GCCGATGGGT CGAAAGTCCA TGCCTCGCGG TAACTTCACA 
CCGGAAGATT ACCTCCTTGT GGCCTTTGAT TTCCCGCTCA GGGTATGTGC CTGGCTTGCG 
CAGATGAAGG CTGGCATGTG GGTGAGGAAT GGGTTGAGCT TGCGTCACCA AGGAGGGACC 
TACCGCGGTG TTAGCCAGAG AGATGTTGCT CACAATAGGG ACGTATTTCT ATTGCAGACC 
GCTCTTGTTG TCTGCGACCC AAGCCGTGTT CTTGCCTCGA TCGTTGACCG CTACGGCATG 
GAGATGTGGA TCAAAGGGTT CTTTGAACAA AAGTCCAAGG CCCAAGACGA TGGTCAACAT 
TTGGATGTTG TGGAAGACAT GCTTCATCTG CTCGTTGTCC TCGTAAGTGA CCGTACATCT 
CTTACGCAAA GTGAGGATGA AGCTAGGCCA CTCAATTCGG CCATGCGTCT CAAAACCAGC 
CCCCTCATCG CGGCCATGCG ACGTGACCTC ACGCACGTCC TTTGCTTTAA ACCGTTGTCG 
TTTTCGGAAA TTTGCAACAA ACTGCCGGAC AAGTTCCACG AGCACGAGGA CTTCCATAAG 
CTGTTGGACG AAATGACCAT CTACAAGGCA CCCGAGGGGC TGACAGATGT TGGCACATTC 
GAGCTTAAGC CAGAGTTCAT TGAGGATGTG GATCCTTACA TCGCGCACTA CAACAAGAAC 
CAACGCGAAG AGTCAGAAAT GGCATGGCGC AAGTGCATGG CTAAGAAGAC TGGGCAGTCG 
ATTGAGGATG TCGTGTATGA GCCCAATCTT CGTCCAATCA CTTCTGGAGT CTTTGCCGGA 
CTGTCTGATT TCACCCGGAC AGGCATGTTC GCCCAGATTA TCTACTATTC CCTGCTCTAT 
ACTCTCTCAT CCTCCAAGTT TACACCCCAG GTGCCCACGA CTCGAGTTGA GACCTTCCTT 
CAAGTTGTCC TGCATCTGGT TTTGATCGCC ATCAAGGAAG ATAAAACAGG TGAGGAGGAT 
GGCCAGCAAC CGTCATTCAT CTCTCGTTCT CTCAGCCACC AGGCGCGCAG CAACTTCATG 
CCAGAGAACC CTACGGCAAA AACCGTCGTG GCACTCTTGA ACCTCCTTGC CGCCGAAGAT 
GCTTTCAAGA CGGTTCACCC CAAGATCCAC TTGATTCTGA AGAAGATGCG GCAACAGCGG 
CCCCATGAGT TCGAGTCTGC TTACCATAGC CTTGGTCTCT CAGTAGACCA TATTGACGCT 
GGTTTGCCCG ACAGCCAGAA CACTGACGAG GAGCGCGAGA AGAAGAAGAA GGCTGCACTA 
GCGCGCCAAG CTCGTGTTAT GGCACAGTTC CAGGCCCAGC AAAAGAGTTT CATGGAGAAG 
CAAGGAGAAA TCGACTGGGG TGATGTGGAC GATATCGATG AGGACGAGGA CATGCCAGCT 
GCCGAGGAGC ATAAAAACTA TTGGAAGTAT CCATCAGGAA CATGCATTCT GTGCCAGGAA 
GATACAGATG ATCGTCGCCT TTACGGATCC TTCGCCTTCT TCACTGAGAG CAACATTTAT 
CGGCAGACTG ATCTCCAGGA TCCGGATTTC GTGAGGGAAG CGTTCAAGAC ACCCGAAAAC 
TTGGACAGGT CTGCAGAGAG TATCCGGCCC TTTGGACTTG CTCATGAGAA CCGCCAGATT 
GTTCAAAAGG TTGACCAGGA GGGAAAGATA TTTGAGGCTG AGCGCTCCGT TATTGGCAAA 
GGCTTCCCAT CGCACCTGTC TCGTTCCGGC CCTGTTTCGA CTGGGTGCGG CCATATCATG 
CATTATGGAT GCTTTGAAGC ATACTTTGAT GCCACTGTAC GCCGTCATAA TCAGCAGATC 
GCAAGACATC CGCCTGAGGA CACGAGTAGG CTGGAGTTTG TATGCCCCTT GTGCAAGGCC 
TTAGGCAATG CATTCCTCCC CATCGTCTGG AAAGGACAAG AAGAATTCTA TCCTGGTCCA 
TTATCACCAT GTGAGTCATT TAACACATGG CTGGACAAAC AGCTGCATTC TGCATATTAT 
GTCCTGGGAG CGGCAAGGCC TCCAGACCAA ATCCAGCATT CGTTTGCAAC GTACACGGCG 
AGAACAATGG TCCACAGTCT TGCTGAAGAT TCAGCTCAAC TGTTTGGCGA TGCCTGGGTA 
GACCTAGGGG CCCGTTCAAG GTCAAGCGGG ACGCCATTCA GTGACTCCTT CTCGATCATC 
TCAGCTACTG AGGTTAGCAG CCGAGGGGCT ACTCCTGCCG AAGGGAACAG CATGATGAGA 
GAGCTGGTCT CTGCGTACCG TCGTCTTCGA GATACATTGC GGAAAAACGG CATCAACTCG 
CACCATGATC TTCCGGAGAC AGTGCGAGAT GATGAGCTGG CTTGCAGTGA TGCTCTTGCT 
CGCTCTGTGG GCTTTTCTAT TGCAGCTATT GAGATTCAGC AGCGCGGGGT GGAAGTGGAC 
TATGGAATGA CCTTACTTGA GAAGATTCCT GAACAAGTCC TTACCCACCT CCGTATTCTG 
ACTGAGACAG TGACGACTTT CATCACTGTA GGCGGACTCA GAGAGGCTGG AGACAACAGG 
ATCGACACCG AGTTTCGGAG AGACAGCGAA CGCCAGCATT GCCAGCTGTT CATTGCACAA 
TATAAGGATG CCGAAACCGA CAGCGCCAGA CAACCTTATC AGGAGTATCC TCCCCTTCTC 
AGCCAAGACC CTTTCGTTTT CTTGTGCGAA TGTGTCTTTG GCGTTGTCCC AGCACAAAGG 
TTTGAAATCG CCCACATCGT GAGGCTTTGC TACCTTGCTG AGATCACCAA AGTGGTTTAC 
CACATGGGCC GCAACATGCC TGCCACTATG TGGATGTCAA AGATCTTCAG CGACCGCAAA 
GACGAAATGT CGCCTGAACT GGCTAACTTC GCTTCATTTT GCGAAACGGT TGTACGGATG 
GACTTGGGCT TTTCCGAGAG GTACAGGGTC CAAAGCTCTA CTGGGGAGAA CAAGGCGTTC 
GACCAGCCTG GATTGGACAG CTGGGAGGGC TGGTACCGGT TCATCAGAAA CTATGCACTT 
ACGTTCCTTC GTAAGTGCGC CATCCTGCTT TACACAAGGT ACAATGTCGA TTTCAACAGC 
CGCGTCTCGC CCAATCCTGA ACAGAAGGAG CTCGAGCGCC TCACCGAGAC GCTGAAACTT 
CCCTCATTCG ATGCAATGCT AGCTTCCCTC ACGCCGGACT CGCGAATTTC CCAGCTCGTA 
TCTGGCTGGA TAGAGCATCA GACTTGCTGG GACGCAGAAC ATTCAACCCT CGCCGCTGAC 
GACAATAAGT TGTTGCCGCC TTCGGCAGTG CTGAGCCACC CGGGCATCTT CGAGCTTGTC 
GGCCTTCCCA AGAACTACGC CACGCTCATT GAGGAGTGTA CGCGGAGGAA GTGTCCTACT 
AAGGGCAAGG ACATTTCTGA CCCTATGCTC TGTCTCTTCT GCGGAGATCT GTTCTGCGGT 
CAAAGCATCT GTTGCGCGGT GGAGGATAGG GAAGGCAGAG GCAAAACCAT GAGGATTGGC 
GGTTGCCAGC AACACATGAG AAAGTGCCAA AAGAACATAG GTCTCTTCCT CAATATCCGA 
AGATGCTGCA TCTTCTACCT CCATCGCCTC TCAGGATCCT TCAGCAACGC GCCCTACATC 
GACAAGTACG GCGAGGTGGA TCTCGGTCTC CGTCACGGAC GGCTGCTTTA TCTTCATCAG 
AAACGGTATG ATTCCATGTT GCGCAATCTG TGGCTCAGTC ATGGTATTCA GAGCTTCATT 
AGCCGTAAGC TTGAGGGGGA CATCAATAAC GGTGGCTGGG AGACACTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_965334.3
CDS	515..6985
Translation	MEAQMSTQEQQLCQALLAMPGKYRYRYSEEASRELLLTLFWSMAGGKNEYMKLFFPQGDPRNTSLKLRDAQGAVEGAEYTEAARGKACGHIFKQGEASYACKTCSADDTCCLCSKCFEATDHTGHMVRISISPGNSGCCDCGDLEAWKRPMFCTIHSMWEGDRDKGKGKATELPEDLVRNIRMTIGRVFDYMCDVISCAPEQLRQTKTVQSIQEDERLSRLSSTYCGGETDSPGEYAVLLWNDEKHTVVDVRDQVARACNTTLANGLKAAYETDAIGRSILMYDSSLEKLLEVAEVLERIRVTVTIRSSRDTFREQMCGTIIEWLRDISGCRVGHDHQLLRNIVCEEMLKPWRKGSPAAHAIVGKDGIDDEDRIDQEENVPSGFYEQQGRLLLQARQAARRMAEAREAGEDEGDEDDEDGDDIIVSGGEMDEDEEGDEDVMMLDEQGDAAGDDVAMTDWREVRVVQVAPAAAGGRPLPPPPPPAPTTATAHRRAIREREHTPDDSDTAEPLIAPNIYTKAHLDIPKTPGSPKAKTSPPNPGRYWLQTPAGYVEESNIPVAEDLFERVRLDWLILFDLRMWKKVRCDLRSLYISTVVTIPEFKRVLGLRFAGLYTTLAQLYLIGDREPDHSIINISLQMLTTPSITAEIVERGNFLTSLMAILYTFLTTRQVGHPWDVASNAVLSFDSGSVTNRRMYHFFLDLKYLFGSPHVQERLRTEERYMMQFLDLVKLHQGICPNVRAVGEHVEYETDSWISASLVTREINRLCRQFAQSFRNVKEHGLQYVNNAIRLAAKVTIINSLGAERHRFNQSEIKEEISFRNLTDFEFEPTGRSFKVVHFVVEEQPNSFHHALHYTLSWLIECGKSMSVDDLRKLLTFETCDLLAKPQPMGRKSMPRGNFTPEDYLLVAFDFPLRVCAWLAQMKAGMWVRNGLSLRHQGGTYRGVSQRDVAHNRDVFLLQTALVVCDPSRVLASIVDRYGMEMWIKGFFEQKSKAQDDGQHLDVVEDMLHLLVVLVSDRTSLTQSEDEARPLNSAMRLKTSPLIAAMRRDLTHVLCFKPLSFSEICNKLPDKFHEHEDFHKLLDEMTIYKAPEGLTDVGTFELKPEFIEDVDPYIAHYNKNQREESEMAWRKCMAKKTGQSIEDVVYEPNLRPITSGVFAGLSDFTRTGMFAQIIYYSLLYTLSSSKFTPQVPTTRVETFLQVVLHLVLIAIKEDKTGEEDGQQPSFISRSLSHQARSNFMPENPTAKTVVALLNLLAAEDAFKTVHPKIHLILKKMRQQRPHEFESAYHSLGLSVDHIDAGLPDSQNTDEEREKKKKAALARQARVMAQFQAQQKSFMEKQGEIDWGDVDDIDEDEDMPAAEEHKNYWKYPSGTCILCQEDTDDRRLYGSFAFFTESNIYRQTDLQDPDFVREAFKTPENLDRSAESIRPFGLAHENRQIVQKVDQEGKIFEAERSVIGKGFPSHLSRSGPVSTGCGHIMHYGCFEAYFDATVRRHNQQIARHPPEDTSRLEFVCPLCKALGNAFLPIVWKGQEEFYPGPLSPCESFNTWLDKQLHSAYYVLGAARPPDQIQHSFATYTARTMVHSLAEDSAQLFGDAWVDLGARSRSSGTPFSDSFSIISATEVSSRGATPAEGNSMMRELVSAYRRLRDTLRKNGINSHHDLPETVRDDELACSDALARSVGFSIAAIEIQQRGVEVDYGMTLLEKIPEQVLTHLRILTETVTTFITVGGLREAGDNRIDTEFRRDSERQHCQLFIAQYKDAETDSARQPYQEYPPLLSQDPFVFLCECVFGVVPAQRFEIAHIVRLCYLAEITKVVYHMGRNMPATMWMSKIFSDRKDEMSPELANFASFCETVVRMDLGFSERYRVQSSTGENKAFDQPGLDSWEGWYRFIRNYALTFLRKCAILLYTRYNVDFNSRVSPNPEQKELERLTETLKLPSFDAMLASLTPDSRISQLVSGWIEHQTCWDAEHSTLAADDNKLLPPSAVLSHPGIFELVGLPKNYATLIEECTRRKCPTKGKDISDPMLCLFCGDLFCGQSICCAVEDREGRGKTMRIGGCQQHMRKCQKNIGLFLNIRRCCIFYLHRLSGSFSNAPYIDKYGEVDLGLRHGRLLYLHQKRYDSMLRNLWLSHGIQSFISRKLEGDINNGGWETL

Target ORF information:

RefSeq Version	XM_960241.3
Organism	Neurospora crassa OR74A
Definition	Neurospora crassa OR74A ubiquitin-protein ligase E3 component (ubr1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_960241.3

ORF Insert Sequence:

ATGGAAGCCC AGATGTCGAC ACAGGAGCAG CAGCTCTGCC AGGCCTTGCT GGCGATGCCT 
GGCAAATACC GATACCGCTA CAGCGAAGAG GCATCGCGGG AACTTCTTCT CACCCTGTTC 
TGGTCCATGG CTGGTGGGAA AAACGAATAC ATGAAGCTCT TCTTTCCCCA AGGGGATCCA 
CGAAACACTT CCCTCAAGCT CAGGGATGCC CAAGGCGCGG TCGAGGGCGC CGAATACACC 
GAAGCCGCGC GTGGCAAGGC CTGCGGTCAC ATATTCAAAC AAGGAGAAGC ATCGTATGCG 
TGCAAGACCT GTAGCGCAGA CGACACCTGC TGCCTGTGCA GCAAGTGCTT CGAAGCCACC 
GACCATACAG GCCATATGGT GCGAATCAGT ATCTCGCCCG GAAACAGCGG ATGTTGCGAT 
TGCGGAGATT TGGAAGCGTG GAAGCGGCCC ATGTTTTGCA CAATACATTC GATGTGGGAG 
GGCGACCGCG ATAAGGGCAA GGGAAAAGCG ACAGAACTTC CAGAGGATCT GGTCAGGAAT 
ATCAGGATGA CCATCGGCCG CGTCTTCGAC TACATGTGCG ACGTCATCTC TTGCGCCCCC 
GAGCAGCTCC GCCAAACCAA AACCGTTCAG TCTATCCAAG AGGATGAAAG GCTCTCGAGG 
TTATCATCGA CATACTGCGG CGGAGAGACT GACTCGCCGG GGGAATATGC CGTTCTGCTG 
TGGAATGATG AAAAGCACAC CGTGGTCGAT GTGCGTGACC AAGTGGCTCG GGCGTGCAAC 
ACCACGCTTG CAAATGGCTT GAAAGCTGCT TACGAGACCG ATGCCATTGG TAGGAGTATC 
CTGATGTACG ACAGCAGTCT GGAAAAGCTG CTCGAAGTAG CCGAGGTACT TGAGAGGATA 
AGGGTTACCG TTACCATTCG CTCTTCCAGG GATACCTTCA GAGAGCAAAT GTGCGGCACC 
ATAATTGAGT GGCTTAGAGA TATCTCTGGC TGCCGAGTTG GCCACGACCA TCAGCTTTTG 
AGAAACATCG TGTGCGAGGA AATGCTTAAG CCTTGGAGGA AAGGTAGCCC CGCGGCACAT 
GCCATTGTCG GCAAAGATGG CATTGACGAT GAAGACCGTA TTGACCAAGA AGAGAACGTA 
CCAAGCGGGT TCTACGAGCA GCAAGGCAGG CTTTTGCTTC AGGCACGTCA AGCTGCCCGT 
AGGATGGCAG AGGCTCGTGA GGCTGGCGAA GATGAAGGGG ATGAAGACGA TGAAGACGGG 
GACGACATCA TTGTCTCTGG TGGAGAAATG GATGAAGATG AGGAGGGGGA CGAAGATGTC 
ATGATGCTTG ACGAACAAGG TGACGCAGCT GGGGATGATG TTGCCATGAC GGACTGGCGA 
GAAGTTAGGG TGGTGCAGGT AGCCCCGGCC GCTGCTGGCG GCCGTCCTCT TCCGCCACCT 
CCCCCTCCGG CACCGACTAC TGCTACGGCT CACCGACGAG CCATTCGGGA ACGCGAGCAC 
ACTCCTGATG ATTCCGATAC CGCCGAGCCA CTCATCGCGC CAAATATCTA CACCAAGGCC 
CACCTCGACA TTCCAAAGAC CCCAGGCAGT CCGAAAGCCA AGACATCGCC TCCAAATCCT 
GGACGTTACT GGTTGCAGAC TCCTGCCGGC TACGTGGAGG AAAGTAACAT TCCGGTTGCC 
GAAGACCTTT TCGAGCGAGT TCGTCTAGAC TGGCTGATAC TATTTGACTT GCGGATGTGG 
AAGAAGGTTC GCTGTGATCT TCGGTCATTA TACATCTCAA CCGTCGTTAC GATCCCCGAG 
TTCAAACGTG TACTCGGCTT GCGGTTTGCT GGCCTTTACA CCACTCTGGC TCAGTTGTAC 
CTCATCGGCG ATCGTGAGCC TGATCACTCC ATCATCAACA TCTCCTTACA GATGCTGACA 
ACTCCCTCCA TTACTGCTGA AATCGTTGAG CGGGGAAATT TCCTTACCAG TCTGATGGCC 
ATTCTTTACA CATTTTTGAC CACTCGTCAA GTCGGACACC CTTGGGACGT GGCAAGCAAC 
GCAGTTTTGT CCTTCGATTC AGGCTCGGTT ACGAACCGGC GCATGTATCA TTTCTTCTTA 
GATCTCAAGT ATCTCTTTGG GTCTCCGCAC GTTCAGGAAC GCCTCCGGAC AGAGGAGCGA 
TATATGATGC AGTTCCTCGA CCTCGTCAAG TTGCATCAAG GTATCTGCCC GAACGTCAGG 
GCTGTCGGCG AACACGTCGA ATATGAGACA GATAGCTGGA TCAGCGCGTC TCTTGTCACA 
CGGGAGATCA ACAGACTTTG CAGACAGTTC GCCCAATCCT TCCGGAACGT CAAGGAACAC 
GGTTTGCAGT ATGTCAACAA TGCAATCAGA CTGGCTGCAA AGGTTACCAT TATCAACTCA 
CTTGGTGCCG AGCGTCACCG GTTTAACCAG TCAGAAATCA AGGAAGAGAT TTCCTTCAGA 
AACCTTACAG ATTTTGAGTT TGAGCCAACT GGGCGAAGTT TCAAGGTCGT CCACTTTGTG 
GTAGAGGAGC AACCCAACAG TTTTCATCAT GCCCTTCACT ACACCTTGTC GTGGCTTATC 
GAGTGCGGCA AATCGATGAG CGTTGACGAC CTGCGCAAGC TCCTTACTTT CGAAACCTGC 
GATTTGCTAG CAAAACCTCA GCCGATGGGT CGAAAGTCCA TGCCTCGCGG TAACTTCACA 
CCGGAAGATT ACCTCCTTGT GGCCTTTGAT TTCCCGCTCA GGGTATGTGC CTGGCTTGCG 
CAGATGAAGG CTGGCATGTG GGTGAGGAAT GGGTTGAGCT TGCGTCACCA AGGAGGGACC 
TACCGCGGTG TTAGCCAGAG AGATGTTGCT CACAATAGGG ACGTATTTCT ATTGCAGACC 
GCTCTTGTTG TCTGCGACCC AAGCCGTGTT CTTGCCTCGA TCGTTGACCG CTACGGCATG 
GAGATGTGGA TCAAAGGGTT CTTTGAACAA AAGTCCAAGG CCCAAGACGA TGGTCAACAT 
TTGGATGTTG TGGAAGACAT GCTTCATCTG CTCGTTGTCC TCGTAAGTGA CCGTACATCT 
CTTACGCAAA GTGAGGATGA AGCTAGGCCA CTCAATTCGG CCATGCGTCT CAAAACCAGC 
CCCCTCATCG CGGCCATGCG ACGTGACCTC ACGCACGTCC TTTGCTTTAA ACCGTTGTCG 
TTTTCGGAAA TTTGCAACAA ACTGCCGGAC AAGTTCCACG AGCACGAGGA CTTCCATAAG 
CTGTTGGACG AAATGACCAT CTACAAGGCA CCCGAGGGGC TGACAGATGT TGGCACATTC 
GAGCTTAAGC CAGAGTTCAT TGAGGATGTG GATCCTTACA TCGCGCACTA CAACAAGAAC 
CAACGCGAAG AGTCAGAAAT GGCATGGCGC AAGTGCATGG CTAAGAAGAC TGGGCAGTCG 
ATTGAGGATG TCGTGTATGA GCCCAATCTT CGTCCAATCA CTTCTGGAGT CTTTGCCGGA 
CTGTCTGATT TCACCCGGAC AGGCATGTTC GCCCAGATTA TCTACTATTC CCTGCTCTAT 
ACTCTCTCAT CCTCCAAGTT TACACCCCAG GTGCCCACGA CTCGAGTTGA GACCTTCCTT 
CAAGTTGTCC TGCATCTGGT TTTGATCGCC ATCAAGGAAG ATAAAACAGG TGAGGAGGAT 
GGCCAGCAAC CGTCATTCAT CTCTCGTTCT CTCAGCCACC AGGCGCGCAG CAACTTCATG 
CCAGAGAACC CTACGGCAAA AACCGTCGTG GCACTCTTGA ACCTCCTTGC CGCCGAAGAT 
GCTTTCAAGA CGGTTCACCC CAAGATCCAC TTGATTCTGA AGAAGATGCG GCAACAGCGG 
CCCCATGAGT TCGAGTCTGC TTACCATAGC CTTGGTCTCT CAGTAGACCA TATTGACGCT 
GGTTTGCCCG ACAGCCAGAA CACTGACGAG GAGCGCGAGA AGAAGAAGAA GGCTGCACTA 
GCGCGCCAAG CTCGTGTTAT GGCACAGTTC CAGGCCCAGC AAAAGAGTTT CATGGAGAAG 
CAAGGAGAAA TCGACTGGGG TGATGTGGAC GATATCGATG AGGACGAGGA CATGCCAGCT 
GCCGAGGAGC ATAAAAACTA TTGGAAGTAT CCATCAGGAA CATGCATTCT GTGCCAGGAA 
GATACAGATG ATCGTCGCCT TTACGGATCC TTCGCCTTCT TCACTGAGAG CAACATTTAT 
CGGCAGACTG ATCTCCAGGA TCCGGATTTC GTGAGGGAAG CGTTCAAGAC ACCCGAAAAC 
TTGGACAGGT CTGCAGAGAG TATCCGGCCC TTTGGACTTG CTCATGAGAA CCGCCAGATT 
GTTCAAAAGG TTGACCAGGA GGGAAAGATA TTTGAGGCTG AGCGCTCCGT TATTGGCAAA 
GGCTTCCCAT CGCACCTGTC TCGTTCCGGC CCTGTTTCGA CTGGGTGCGG CCATATCATG 
CATTATGGAT GCTTTGAAGC ATACTTTGAT GCCACTGTAC GCCGTCATAA TCAGCAGATC 
GCAAGACATC CGCCTGAGGA CACGAGTAGG CTGGAGTTTG TATGCCCCTT GTGCAAGGCC 
TTAGGCAATG CATTCCTCCC CATCGTCTGG AAAGGACAAG AAGAATTCTA TCCTGGTCCA 
TTATCACCAT GTGAGTCATT TAACACATGG CTGGACAAAC AGCTGCATTC TGCATATTAT 
GTCCTGGGAG CGGCAAGGCC TCCAGACCAA ATCCAGCATT CGTTTGCAAC GTACACGGCG 
AGAACAATGG TCCACAGTCT TGCTGAAGAT TCAGCTCAAC TGTTTGGCGA TGCCTGGGTA 
GACCTAGGGG CCCGTTCAAG GTCAAGCGGG ACGCCATTCA GTGACTCCTT CTCGATCATC 
TCAGCTACTG AGGTTAGCAG CCGAGGGGCT ACTCCTGCCG AAGGGAACAG CATGATGAGA 
GAGCTGGTCT CTGCGTACCG TCGTCTTCGA GATACATTGC GGAAAAACGG CATCAACTCG 
CACCATGATC TTCCGGAGAC AGTGCGAGAT GATGAGCTGG CTTGCAGTGA TGCTCTTGCT 
CGCTCTGTGG GCTTTTCTAT TGCAGCTATT GAGATTCAGC AGCGCGGGGT GGAAGTGGAC 
TATGGAATGA CCTTACTTGA GAAGATTCCT GAACAAGTCC TTACCCACCT CCGTATTCTG 
ACTGAGACAG TGACGACTTT CATCACTGTA GGCGGACTCA GAGAGGCTGG AGACAACAGG 
ATCGACACCG AGTTTCGGAG AGACAGCGAA CGCCAGCATT GCCAGCTGTT CATTGCACAA 
TATAAGGATG CCGAAACCGA CAGCGCCAGA CAACCTTATC AGGAGTATCC TCCCCTTCTC 
AGCCAAGACC CTTTCGTTTT CTTGTGCGAA TGTGTCTTTG GCGTTGTCCC AGCACAAAGG 
TTTGAAATCG CCCACATCGT GAGGCTTTGC TACCTTGCTG AGATCACCAA AGTGGTTTAC 
CACATGGGCC GCAACATGCC TGCCACTATG TGGATGTCAA AGATCTTCAG CGACCGCAAA 
GACGAAATGT CGCCTGAACT GGCTAACTTC GCTTCATTTT GCGAAACGGT TGTACGGATG 
GACTTGGGCT TTTCCGAGAG GTACAGGGTC CAAAGCTCTA CTGGGGAGAA CAAGGCGTTC 
GACCAGCCTG GATTGGACAG CTGGGAGGGC TGGTACCGGT TCATCAGAAA CTATGCACTT 
ACGTTCCTTC GTAAGTGCGC CATCCTGCTT TACACAAGGT ACAATGTCGA TTTCAACAGC 
CGCGTCTCGC CCAATCCTGA ACAGAAGGAG CTCGAGCGCC TCACCGAGAC GCTGAAACTT 
CCCTCATTCG ATGCAATGCT AGCTTCCCTC ACGCCGGACT CGCGAATTTC CCAGCTCGTA 
TCTGGCTGGA TAGAGCATCA GACTTGCTGG GACGCAGAAC ATTCAACCCT CGCCGCTGAC 
GACAATAAGT TGTTGCCGCC TTCGGCAGTG CTGAGCCACC CGGGCATCTT CGAGCTTGTC 
GGCCTTCCCA AGAACTACGC CACGCTCATT GAGGAGTGTA CGCGGAGGAA GTGTCCTACT 
AAGGGCAAGG ACATTTCTGA CCCTATGCTC TGTCTCTTCT GCGGAGATCT GTTCTGCGGT 
CAAAGCATCT GTTGCGCGGT GGAGGATAGG GAAGGCAGAG GCAAAACCAT GAGGATTGGC 
GGTTGCCAGC AACACATGAG AAAGTGCCAA AAGAACATAG GTCTCTTCCT CAATATCCGA 
AGATGCTGCA TCTTCTACCT CCATCGCCTC TCAGGATCCT TCAGCAACGC GCCCTACATC 
GACAAGTACG GCGAGGTGGA TCTCGGTCTC CGTCACGGAC GGCTGCTTTA TCTTCATCAG 
AAACGGTATG ATTCCATGTT GCGCAATCTG TGGCTCAGTC ATGGTATTCA GAGCTTCATT 
AGCCGTAAGC TTGAGGGGGA CATCAATAAC GGTGGCTGGG AGACACTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Nature422(6934)859-68(2003 Apr)
Galagan JE,Calvo SE,Borkovich KA,Selker EU,Read ND,Jaffe D,FitzHugh W,Ma LJ,Smirnov S,Purcell S,Rehman B,Elkins T,Engels R,Wang S,Nielsen CB,Butler J,Endrizzi M,Qui D,Ianakiev P,Bell-Pedersen D,Nelson MA,Werner-Washburne M,Selitrennikoff CP,Kinsey JA,Braun EL,Zelter A,Schulte U,Kothe GO,Jedd G,Mewes W,Staben C,Marcotte E,Greenberg D,Roy A,Foley K,Naylor J,Stange-Thomann N,Barrett R,Gnerre S,Kamal M,Kamvysselis M,Mauceli E,Bielke C,Rudd S,Frishman D,Krystofova S,Rasmussen C,Metzenberg RL,Perkins DD,Kroken S,Cogoni C,Macino G,Catcheside D,Li W,Pratt RJ,Osmani SA,DeSouza CP,Glass L,Orbach MJ,Berglund JA,Voelker R,Yarden O,Plamann M,Seiler S,Dunlap J,Radford A,Aramayo R,Natvig DO,Alex LA,Mannhaupt G,Ebbole DJ,Freitag M,Paulsen I,Sachs MS,Lander ES,Nusbaum C,Birren B

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ubr1 cDNA ORF clone, Neurospora crassa OR74A

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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