NCU03116 cDNA ORF clone, Neurospora crassa OR74A

Gene
Clones
gRNAs

Summary

Gene Symbol	NCU03116
Entrez Gene ID	3881133
Full Name	Ras GTPase activating protein
Gene Type	protein-coding
Organism	Neurospora crassa OR74A

mRNA and Protein(s)

mRNA	Protein	Name
XM_011394782.1	XP_011393084.1	Ras GTPase activating protein, variant 1
XM_011394785.1	XP_011393087.1	Ras GTPase activating protein, variant 3
XM_011394783.1	XP_011393085.1	Ras GTPase activating protein
XM_011394784.1	XP_011393086.1	Ras GTPase activating protein, variant 2

Pathways from BioSystems

Homologies

Schizosaccharomyces pombe (fission yeast)	rng2	NP_593860.1
Magnaporthe oryzae (rice blast fungus)	MGG_03700	XP_003716181.1
Kluyveromyces lactis	KLLA0D12914g	XP_453638.1
Neurospora crassa	NCU03116	XP_964975.1
Saccharomyces cerevisiae (baker's yeast)	IQG1	NP_015082.1
Eremothecium gossypii	AGOS_AFL150C	NP_985400.2

Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Galagan JE, Calvo SE, Borkovich KA, Selker EU, Read ND, Jaffe D, FitzHugh W, Ma LJ, Smirnov S, Purcell S, Rehman B, Elkins T, Engels R, Wang S, Nielsen CB, Butler J, Endrizzi M, Qui D, Ianakiev P, Bell-Pedersen D, Nelson MA, Werner-Washburne M, Selitrennikoff CP, Kinsey JA, Braun EL, Zelter A, Schulte U, Kothe GO, Jedd G, Mewes W, Staben C, Marcotte E, Greenberg D, Roy A, Foley K, Naylor J, Stange-Thomann N, Barrett R, Gnerre S, Kamal M, Kamvysselis M, Mauceli E, Bielke C, Rudd S, Frishman D, Krystofova S, Rasmussen C, Metzenberg RL, Perkins DD, Kroken S, Cogoni C, Macino G, Catcheside D, Li W, Pratt RJ, Osmani SA, DeSouza CP, Glass L, Orbach MJ, Berglund JA, Voelker R, Yarden O, Plamann M, Seiler S, Dunlap J, Radford A, Aramayo R, Natvig DO, Alex LA, Mannhaupt G, Ebbole DJ, Freitag M, Paulsen I, Sachs MS, Lander ES, Nusbaum C, Birren B
Nature422(6934)859-68(2003 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following NCU03116 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NCU03116 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ONf13025	XM_011394782.1 Latest version!	Neurospora crassa OR74A Ras GTPase activating protein, variant 1 (NCU03116), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ONf13025	XM_011394785.1 Latest version!	Neurospora crassa OR74A Ras GTPase activating protein, variant 3 (NCU03116), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ONf13025	XM_011394784.1 Latest version!	Neurospora crassa OR74A Ras GTPase activating protein, variant 2 (NCU03116), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ONf13025	XM_011394783.1 Latest version!	Neurospora crassa OR74A Ras GTPase activating protein (NCU03116), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NCU03116 ( XM_011394782.1 ) cDNA ORF clone, Neurospora crassa OR74A -> XP_011393084.1 Neurospora crassa OR74A Ras GTPase activating protein, variant 1 (NCU03116), mRNA.

Price & Availability↑

CloneID	ONf13025
Clone ID Related Accession (Same CDS sequence)	XM_011394782.1 , XM_011394785.1 , XM_011394783.1 , XM_011394784.1
Accession Version	XM_011394782.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5400bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-10
Organism	Neurospora crassa OR74A
Product	Ras GTPase activating protein, variant 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026501).

ATGTCGTCGT ACAAACCACA CCCCCTTCGT CAGACACAGA CCGCCGACTA CTCGGTTAAT 
AGACAATCCT CCACCACATC AACATCATCA TCAGGGTACT CGGCCTCTTC CATAGGACAG 
TCGGATATCA GTCGACCCAT TAGCTCCTTG TCTACAGCAA CCAGCGTCTA CTCCCAATAT 
GGACACAAGC GGGGCCAAAG CGAGGCGAGC CGACTACCCC CAAGCCTGTC TGGTGGCTCA 
CACACCCTCC CGTCCACGCC GACAAGTGAC AATATCTACA AGAATGCCCG CCAGTCCCTC 
CGACCGACAT CTCAAGCCTC TACGGGAGCA GCGCCCGCCA GCAGCAGCAT ATCGACTGCG 
GGTTCGAGAA TCGCGCCGCG CACGCCCACA CCAGGCCACC ACGATCGTGG AAAAAGTGTT 
GACATCAGCA AGCTGTCCAT CGGAGAGTAT GGGACCTCGA CTACCCATCG CGAACGCGGT 
CAGAGTCTCG ACAACGGCAA GCCTCTGGCG ACTGAGCGCC CACGGATATC GCCTACGGGA 
ACTGTTCATG AACGTCTAGC AAGCTTCGAT GTCAGCAAGT CCTCGGTACC GGAACGTCCG 
CGGATATCGC CTACGGGTGC CCTCCACGAG CGCGGTCACA CTATCGATAT GGGCAAATTA 
TCGATTAGCG ATAATACACG TACTTCGTCA GGCATGTCTC CGACCGTGAC ATCGCCTCCG 
GCAACTTCTC CAACGACCCC TTCAACGCGA AGGCCGGCTT CCATGTTTCT ATCCAGATCA 
GATTCTGTGC GTGGCGGAAG TGACAATGGG TCAATGGCTC TGGTACACTC GAGTCCCATT 
GCCCTTGCTC GTCCGGATCT GGAAAAATTA GGTCGTTCAT CGACAAGCCA ACTCCGAACA 
CTTTCTCAGT TCGCACAAGA TGGAACGGCG GGAGAGCTTG CCATCATATC TCCGTCGCAG 
GAAGTTGTTG GTCTTCGCGG ACGCCGTAGG TTGCAAAGAG CTGACAAGTC TGTAGCGGCG 
CGTCAAAAGA ATGGCGGCTT TGGTTGGGAG ACGAAGTGGA TGGACAAGCA ACGGCAGTTT 
CTACAGGCTT ATGAATACCT TTGCCACATT GGTGAAGCGA AGGAATGGAT TGAGGATATC 
ATACAGCGTC CGATCCCCCC GATTATCGAA TTAGAGGAGG CTTTGCGAGA TGGAGTTACT 
TTGGCCGAAG TGGTCGAATC CCTTAACCCT GAAAAGAAGT TCCGCATTTT CCGCCATCCT 
AAGCTTCAAT ACCGCCATTC AGACAATATC GCCATCTTCT TCCGTTACCT TGATGAAGTC 
GAACTTCCCG ACCTCTTCCG ATTTGAGCTT ATCGATCTGT ACGAGAAAAA GAACATCCCG 
AAAGTTATTT ACTGTCTCCA TGCTTTGAGT TGGCTTCTCT TCCGGAAAGG AATTGTCGAT 
TTCCGCATCG GGAATCTGGT CGGCCAGCTT GAGTTTGAAG ACCATGAGCT GGAGGCGATG 
CAGAAAGGCC TCGATAAGCT GGGGGCTAGT ATGCCTAGCT TCGGCAATAT GGGTGCCGAC 
TTTGGAATTG AGCCAGCTGA ACCCGAGGAG ACGGAGGAGG AACGCATTGA GAGGGAACTG 
GCCGAAAACG AAGAGGCCAT TGTAGAGTTA CAAGCCCAGG CCAGAGCGGC GCTTCAAAGG 
ATACAGCTTG GCTACACTAT GCAACATCTG TGGGATAATG AAGACTGGAT CATTGATCTC 
CAGTCTCGTA TTCGTGGTGA CTGGGCTCGC CAGGTGGCGG ATTACAGGCT CCAGATGAAG 
AATTTCGCGG TCCAGCTTCA AAGCGCAGCG CGCGGTTTCC TCGCCCGCTA TCGACATGCA 
GAAACAAAGG CACAAACAAG TTTTGCGATC GAGTTGCAGA AGACAGCTCG TGGATTTATC 
GCTCGTCAGC AATATGCACA AACATGTTTC ACGATTCAGC TACAAAAAGC GGCGCGTGGA 
TATCTTGCTC GTCGCCAGCA GACACAAACC GAGGAGCAGT TCAAGGGCAA CGAGGATGTG 
GTCTTGAAAC TCCAAAGTAT GGTCAGAGCG GCCAAGGTCC GCAATGTGAT TCGGGAGCAG 
CATTCACAGT TGGAGACGGC AGCGGGACCC CTGCGAGACA TTCAAGCAAC ATTTCGAGGA 
TTTCTGGCTC GAAAGCAGCT CGCTGTGCAA CATGAAAAGA CAGAGAGCGA AGGCGCTACG 
ATTGCTCAAC TTCAGAGCGC AGTTCGTGGT ATGATATTGA GGAAGAATCT GGAGGAAGAA 
AGAGCATCCT TGGAGAGCAA CATCGAAGCC ATCACTAGTC TTCAGGCTGC TGTAAGGGCC 
ATGAAAGCAA GAACTGAGGT CGATGCCCAG CTACAAGCGC TTGAAGGCTT TGCGCCACAA 
TGGACAGCTA TTCAAGCTGC CTTCCGCGGC AAGGTTGCTA GGTCCGACGT CGACGCTACT 
AAAGAAGAGT TGCAGAAGCA TTCAACTGAG ATGGGCCTTC TTCAGTCCCA CATTAGGGCA 
GCGGCAGTGC GGAGGGACAT ATCAGAGCAG CTTGAAAGCA TCGGGGAAAC GGAGGATGCC 
ATCATTGATC TTCAGTCTAT GGTCCGAGGT ATGCTTGAAC GTATGAGGTT ATCGGACGAT 
CTGGATGCTC TTACTCAGGA GACACCAAAC ATCATTGACC TTCAAGCACA CATCCGCGGA 
TGTCTTTATC GCAAGGAGTG TGGCGAGTTG TTGGATGAGC TTGCAGTCAA TACCGAGGAG 
ATCACCGAAC TGCAAGCCCT TGCTCGGGCC ATGTTGCTTC GGAGCGATGT TGCAATGGTC 
TTGGATGAAC TGGAACAAAA CGAGGAGTCT GTAGCGGCGT TCCAGGCTGC TGCCAAGGCT 
TTCGTGGTCA GAGCCAAGTA CGAGGAGAAG AAGCGCTTCT TCCGGGAGAA CATGCAAAAG 
GTTGTCAAGA TTCAGAGTTT CGTACGAGCC AAGCTTCAGG GTGAAGCCTA CAAGAGCTTG 
ACATCTGGAA AGAACCCACC AGTCAACGCC GTCAAGAACT TTGTCCATCT TCTGAACGAC 
AGCGATTTTG ACTTCAATGA GGAGGTAGAG TTTGAGCGCC TACGAAAGAC TGTTGTCCAA 
CAAGTTCGGC AAAACGAGAT GCTGGAACAA TACATCGACC AGCTAGACAT CAAGATCGCA 
CTTCTCGTCA AGAATAAAAT TACCCTTGAT GAAGTTGTCA GACATCAGCA CAACTATGGC 
GGACACGCAA ACAGCCTTCT CGCCAACAGT GTTATTTCAA ATGCTAATCA GTTTGACCTC 
AAGGCTTTGA ATAAGAGTTC CCGCAAGAAG CTTGAGTCCT ACCAGCAGCT GTTCTTCACC 
CTTCAGACGC AACCTCAGTA CCTTGCTAAG CTCTTCAGGC GTATCCGCGA GCAAGGAATA 
ACGGAAAAGG AGTTAAAGCG GATTGAACAC TTGATGATGG GGCTGTTCGG CTATGCGCAA 
AAGCGGAGAG AGGAATACTA CTTATTGAAG CTTATTTCCA GGTCGATAAG GGAAGAAGTC 
GAAGCATGTG CCGCTCTGCA GGATTATCTG AGGGGCAACT ACTTCTGGAC CAAGCTGCTC 
CAGAATTACA CCCGATCCCC TCGTGATAGG AAATATCTCA GAGATCTCTT GGGACCCCTC 
ATCAGAGACA ACATTATCGA GGATCCAGCG CTGGATCTTG AAAGCGACCC CATGCAGATC 
TACCGGTCTG CTATCAACAA TGAGGAGTTG AGGACCGGTC GGCCCAGCCA AAGACCTCTG 
GATGTCCCTC GTGAGGTTGC CATCAAGGAT CCTGAAACCA GAGAGCTCTT CATTGATCAT 
CTGCGGGATC TCCGAGAGAT CTGCGATCAG TTTCTCCTTG CCCTGGAGGA CCTGCTTCCC 
CGCATGCCCT ACGGCCTGAG ATTTGTGTGC CGGCAAACGT ACGAAGCTCT CTGCCAGCAT 
TTCCAGAGGG ACCACCCGCA AAATGTGCTC CAGTTTGTAA GCAACTGGCT CTGGCGCTTC 
TACCTCCAAC CTGCTCTCCT TGGCCCAGAG TCATATGGAG TCACGGAAAA GCAAATGAGT 
CCCCTCCAGA AGCGTAACCT CGGCGAGGTT GCAAAAGTCC TGGGCCAGAT TGCATCAGGC 
AGACCATTTG GCGGAGAGAA CATCTATCTC CAGCCTCTCA ATGCTTTCGT CGCCGAAGCA 
ATCGAGCGCT TGGCTCACAT CACCAATTCC CTTATCGATG TACCTGACGC TGAGAAGACA 
TTCGACATTG ACGAGTTCAA CGATCTCTAC GCCAAGAACA AGCCAACACT GTACATCAAG 
ATGGCGGATA TCTTTTCCAT CCACAATCTC ATCGCTGCCG AGTTGCCTTA TCTGTGCCCG 
AGCAGAGATG ATATGCTGCG CGAGATCATT CAAGATCTCG GTAACGCCAA GAATAATGAG 
AATGAGATGA CCGCAGGCGG GGCCGCTGAT ATTCAGCTGT TCCTGACTCC CAAACTGCAC 
GATATGGAGG ATCCAGAAGC TGAGACTAAG GGGCTGTTTA TGGAGACCAA GAGGTGCATT 
CTGTACATCA TCCGGGTGCA AACGGGTAAC AACCTGCTTG AGATTCTTGT CAAGCCAATT 
ACCCCGGAGG ATGAGCAGAA GTGGAGGACA GTTCTGCGCG AAGACTTTGG TGACGACTCG 
AACACCCGAG GCGCTTATTC GGAGGCGAAC ATGATTGATG TTACGCGCAT GAGTTACTAC 
GACCTAAAGA GGACAGCACT CGAGAACATC CTCAGGTTGG AGCAGATGGG TCGTATCTCC 
AAGCAAAACT ACTACCAGGA TATCCTCAAC GCCATTGCGC TGGACATTCG CACAAAGAGC 
AGGAGAAGGG TTCAGAGACA GAGAGAGCTC GAGGGTGTCC GTCAGACACT CGGCAATCTT 
CACGAGAAGG CAAAGTATCT GGAGCAACAG CGCAAGAGCT ACGATGATTA CATCGAGCAA 
GCCATGGCTA CGCTGCAGAA CAAGAAGGGC AAGAAACGTT TCCTTCTCCC CTTCACCAAG 
CAATACAACC ACCAGCGCGA GCTCGAGCGC TCCGGCCGCG TCCCCAAGTT CGGCTCCTTC 
AAGTACTCGG CCCGCGCCCT CTCGGACAAG GGCGTGCTGG TCTCGTGGAC GGGCATCCCC 
GAGCGCGACT GGGGTCAAAT CAACATCACC ATCTCGTGCG ACGAGGTCGG CATTTTTAGT 
CTTGAGGGCA GCCGCGGCCA CATCCAGATG CCCGGTGCCA GCGCCCTGGT GCCCATCGAG 
GACTTGCTAC AGGCGCAATT TGAGGCGCAT CAGTTTATGA ATTTGTTTGA GGGGCAGCTG 
AGGCTGAACG TGAATTTGTT GTTGCATTTG TTGTACAAGA AGTTCTATCG GACTCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011393084.1
CDS	334..5733
Translation	MSSYKPHPLRQTQTADYSVNRQSSTTSTSSSGYSASSIGQSDISRPISSLSTATSVYSQYGHKRGQSEASRLPPSLSGGSHTLPSTPTSDNIYKNARQSLRPTSQASTGAAPASSSISTAGSRIAPRTPTPGHHDRGKSVDISKLSIGEYGTSTTHRERGQSLDNGKPLATERPRISPTGTVHERLASFDVSKSSVPERPRISPTGALHERGHTIDMGKLSISDNTRTSSGMSPTVTSPPATSPTTPSTRRPASMFLSRSDSVRGGSDNGSMALVHSSPIALARPDLEKLGRSSTSQLRTLSQFAQDGTAGELAIISPSQEVVGLRGRRRLQRADKSVAARQKNGGFGWETKWMDKQRQFLQAYEYLCHIGEAKEWIEDIIQRPIPPIIELEEALRDGVTLAEVVESLNPEKKFRIFRHPKLQYRHSDNIAIFFRYLDEVELPDLFRFELIDLYEKKNIPKVIYCLHALSWLLFRKGIVDFRIGNLVGQLEFEDHELEAMQKGLDKLGASMPSFGNMGADFGIEPAEPEETEEERIERELAENEEAIVELQAQARAALQRIQLGYTMQHLWDNEDWIIDLQSRIRGDWARQVADYRLQMKNFAVQLQSAARGFLARYRHAETKAQTSFAIELQKTARGFIARQQYAQTCFTIQLQKAARGYLARRQQTQTEEQFKGNEDVVLKLQSMVRAAKVRNVIREQHSQLETAAGPLRDIQATFRGFLARKQLAVQHEKTESEGATIAQLQSAVRGMILRKNLEEERASLESNIEAITSLQAAVRAMKARTEVDAQLQALEGFAPQWTAIQAAFRGKVARSDVDATKEELQKHSTEMGLLQSHIRAAAVRRDISEQLESIGETEDAIIDLQSMVRGMLERMRLSDDLDALTQETPNIIDLQAHIRGCLYRKECGELLDELAVNTEEITELQALARAMLLRSDVAMVLDELEQNEESVAAFQAAAKAFVVRAKYEEKKRFFRENMQKVVKIQSFVRAKLQGEAYKSLTSGKNPPVNAVKNFVHLLNDSDFDFNEEVEFERLRKTVVQQVRQNEMLEQYIDQLDIKIALLVKNKITLDEVVRHQHNYGGHANSLLANSVISNANQFDLKALNKSSRKKLESYQQLFFTLQTQPQYLAKLFRRIREQGITEKELKRIEHLMMGLFGYAQKRREEYYLLKLISRSIREEVEACAALQDYLRGNYFWTKLLQNYTRSPRDRKYLRDLLGPLIRDNIIEDPALDLESDPMQIYRSAINNEELRTGRPSQRPLDVPREVAIKDPETRELFIDHLRDLREICDQFLLALEDLLPRMPYGLRFVCRQTYEALCQHFQRDHPQNVLQFVSNWLWRFYLQPALLGPESYGVTEKQMSPLQKRNLGEVAKVLGQIASGRPFGGENIYLQPLNAFVAEAIERLAHITNSLIDVPDAEKTFDIDEFNDLYAKNKPTLYIKMADIFSIHNLIAAELPYLCPSRDDMLREIIQDLGNAKNNENEMTAGGAADIQLFLTPKLHDMEDPEAETKGLFMETKRCILYIIRVQTGNNLLEILVKPITPEDEQKWRTVLREDFGDDSNTRGAYSEANMIDVTRMSYYDLKRTALENILRLEQMGRISKQNYYQDILNAIALDIRTKSRRRVQRQRELEGVRQTLGNLHEKAKYLEQQRKSYDDYIEQAMATLQNKKGKKRFLLPFTKQYNHQRELERSGRVPKFGSFKYSARALSDKGVLVSWTGIPERDWGQINITISCDEVGIFSLEGSRGHIQMPGASALVPIEDLLQAQFEAHQFMNLFEGQLRLNVNLLLHLLYKKFYRTQ

Target ORF information:

RefSeq Version	XM_011394782.1
Organism	Neurospora crassa OR74A
Definition	Neurospora crassa OR74A Ras GTPase activating protein, variant 1 (NCU03116), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011394782.1

ORF Insert Sequence:

ATGTCGTCGT ACAAACCACA CCCCCTTCGT CAGACACAGA CCGCCGACTA CTCGGTTAAT 
AGACAATCCT CCACCACATC AACATCATCA TCAGGGTACT CGGCCTCTTC CATAGGACAG 
TCGGATATCA GTCGACCCAT TAGCTCCTTG TCTACAGCAA CCAGCGTCTA CTCCCAATAT 
GGACACAAGC GGGGCCAAAG CGAGGCGAGC CGACTACCCC CAAGCCTGTC TGGTGGCTCA 
CACACCCTCC CGTCCACGCC GACAAGTGAC AATATCTACA AGAATGCCCG CCAGTCCCTC 
CGACCGACAT CTCAAGCCTC TACGGGAGCA GCGCCCGCCA GCAGCAGCAT ATCGACTGCG 
GGTTCGAGAA TCGCGCCGCG CACGCCCACA CCAGGCCACC ACGATCGTGG AAAAAGTGTT 
GACATCAGCA AGCTGTCCAT CGGAGAGTAT GGGACCTCGA CTACCCATCG CGAACGCGGT 
CAGAGTCTCG ACAACGGCAA GCCTCTGGCG ACTGAGCGCC CACGGATATC GCCTACGGGA 
ACTGTTCATG AACGTCTAGC AAGCTTCGAT GTCAGCAAGT CCTCGGTACC GGAACGTCCG 
CGGATATCGC CTACGGGTGC CCTCCACGAG CGCGGTCACA CTATCGATAT GGGCAAATTA 
TCGATTAGCG ATAATACACG TACTTCGTCA GGCATGTCTC CGACCGTGAC ATCGCCTCCG 
GCAACTTCTC CAACGACCCC TTCAACGCGA AGGCCGGCTT CCATGTTTCT ATCCAGATCA 
GATTCTGTGC GTGGCGGAAG TGACAATGGG TCAATGGCTC TGGTACACTC GAGTCCCATT 
GCCCTTGCTC GTCCGGATCT GGAAAAATTA GGTCGTTCAT CGACAAGCCA ACTCCGAACA 
CTTTCTCAGT TCGCACAAGA TGGAACGGCG GGAGAGCTTG CCATCATATC TCCGTCGCAG 
GAAGTTGTTG GTCTTCGCGG ACGCCGTAGG TTGCAAAGAG CTGACAAGTC TGTAGCGGCG 
CGTCAAAAGA ATGGCGGCTT TGGTTGGGAG ACGAAGTGGA TGGACAAGCA ACGGCAGTTT 
CTACAGGCTT ATGAATACCT TTGCCACATT GGTGAAGCGA AGGAATGGAT TGAGGATATC 
ATACAGCGTC CGATCCCCCC GATTATCGAA TTAGAGGAGG CTTTGCGAGA TGGAGTTACT 
TTGGCCGAAG TGGTCGAATC CCTTAACCCT GAAAAGAAGT TCCGCATTTT CCGCCATCCT 
AAGCTTCAAT ACCGCCATTC AGACAATATC GCCATCTTCT TCCGTTACCT TGATGAAGTC 
GAACTTCCCG ACCTCTTCCG ATTTGAGCTT ATCGATCTGT ACGAGAAAAA GAACATCCCG 
AAAGTTATTT ACTGTCTCCA TGCTTTGAGT TGGCTTCTCT TCCGGAAAGG AATTGTCGAT 
TTCCGCATCG GGAATCTGGT CGGCCAGCTT GAGTTTGAAG ACCATGAGCT GGAGGCGATG 
CAGAAAGGCC TCGATAAGCT GGGGGCTAGT ATGCCTAGCT TCGGCAATAT GGGTGCCGAC 
TTTGGAATTG AGCCAGCTGA ACCCGAGGAG ACGGAGGAGG AACGCATTGA GAGGGAACTG 
GCCGAAAACG AAGAGGCCAT TGTAGAGTTA CAAGCCCAGG CCAGAGCGGC GCTTCAAAGG 
ATACAGCTTG GCTACACTAT GCAACATCTG TGGGATAATG AAGACTGGAT CATTGATCTC 
CAGTCTCGTA TTCGTGGTGA CTGGGCTCGC CAGGTGGCGG ATTACAGGCT CCAGATGAAG 
AATTTCGCGG TCCAGCTTCA AAGCGCAGCG CGCGGTTTCC TCGCCCGCTA TCGACATGCA 
GAAACAAAGG CACAAACAAG TTTTGCGATC GAGTTGCAGA AGACAGCTCG TGGATTTATC 
GCTCGTCAGC AATATGCACA AACATGTTTC ACGATTCAGC TACAAAAAGC GGCGCGTGGA 
TATCTTGCTC GTCGCCAGCA GACACAAACC GAGGAGCAGT TCAAGGGCAA CGAGGATGTG 
GTCTTGAAAC TCCAAAGTAT GGTCAGAGCG GCCAAGGTCC GCAATGTGAT TCGGGAGCAG 
CATTCACAGT TGGAGACGGC AGCGGGACCC CTGCGAGACA TTCAAGCAAC ATTTCGAGGA 
TTTCTGGCTC GAAAGCAGCT CGCTGTGCAA CATGAAAAGA CAGAGAGCGA AGGCGCTACG 
ATTGCTCAAC TTCAGAGCGC AGTTCGTGGT ATGATATTGA GGAAGAATCT GGAGGAAGAA 
AGAGCATCCT TGGAGAGCAA CATCGAAGCC ATCACTAGTC TTCAGGCTGC TGTAAGGGCC 
ATGAAAGCAA GAACTGAGGT CGATGCCCAG CTACAAGCGC TTGAAGGCTT TGCGCCACAA 
TGGACAGCTA TTCAAGCTGC CTTCCGCGGC AAGGTTGCTA GGTCCGACGT CGACGCTACT 
AAAGAAGAGT TGCAGAAGCA TTCAACTGAG ATGGGCCTTC TTCAGTCCCA CATTAGGGCA 
GCGGCAGTGC GGAGGGACAT ATCAGAGCAG CTTGAAAGCA TCGGGGAAAC GGAGGATGCC 
ATCATTGATC TTCAGTCTAT GGTCCGAGGT ATGCTTGAAC GTATGAGGTT ATCGGACGAT 
CTGGATGCTC TTACTCAGGA GACACCAAAC ATCATTGACC TTCAAGCACA CATCCGCGGA 
TGTCTTTATC GCAAGGAGTG TGGCGAGTTG TTGGATGAGC TTGCAGTCAA TACCGAGGAG 
ATCACCGAAC TGCAAGCCCT TGCTCGGGCC ATGTTGCTTC GGAGCGATGT TGCAATGGTC 
TTGGATGAAC TGGAACAAAA CGAGGAGTCT GTAGCGGCGT TCCAGGCTGC TGCCAAGGCT 
TTCGTGGTCA GAGCCAAGTA CGAGGAGAAG AAGCGCTTCT TCCGGGAGAA CATGCAAAAG 
GTTGTCAAGA TTCAGAGTTT CGTACGAGCC AAGCTTCAGG GTGAAGCCTA CAAGAGCTTG 
ACATCTGGAA AGAACCCACC AGTCAACGCC GTCAAGAACT TTGTCCATCT TCTGAACGAC 
AGCGATTTTG ACTTCAATGA GGAGGTAGAG TTTGAGCGCC TACGAAAGAC TGTTGTCCAA 
CAAGTTCGGC AAAACGAGAT GCTGGAACAA TACATCGACC AGCTAGACAT CAAGATCGCA 
CTTCTCGTCA AGAATAAAAT TACCCTTGAT GAAGTTGTCA GACATCAGCA CAACTATGGC 
GGACACGCAA ACAGCCTTCT CGCCAACAGT GTTATTTCAA ATGCTAATCA GTTTGACCTC 
AAGGCTTTGA ATAAGAGTTC CCGCAAGAAG CTTGAGTCCT ACCAGCAGCT GTTCTTCACC 
CTTCAGACGC AACCTCAGTA CCTTGCTAAG CTCTTCAGGC GTATCCGCGA GCAAGGAATA 
ACGGAAAAGG AGTTAAAGCG GATTGAACAC TTGATGATGG GGCTGTTCGG CTATGCGCAA 
AAGCGGAGAG AGGAATACTA CTTATTGAAG CTTATTTCCA GGTCGATAAG GGAAGAAGTC 
GAAGCATGTG CCGCTCTGCA GGATTATCTG AGGGGCAACT ACTTCTGGAC CAAGCTGCTC 
CAGAATTACA CCCGATCCCC TCGTGATAGG AAATATCTCA GAGATCTCTT GGGACCCCTC 
ATCAGAGACA ACATTATCGA GGATCCAGCG CTGGATCTTG AAAGCGACCC CATGCAGATC 
TACCGGTCTG CTATCAACAA TGAGGAGTTG AGGACCGGTC GGCCCAGCCA AAGACCTCTG 
GATGTCCCTC GTGAGGTTGC CATCAAGGAT CCTGAAACCA GAGAGCTCTT CATTGATCAT 
CTGCGGGATC TCCGAGAGAT CTGCGATCAG TTTCTCCTTG CCCTGGAGGA CCTGCTTCCC 
CGCATGCCCT ACGGCCTGAG ATTTGTGTGC CGGCAAACGT ACGAAGCTCT CTGCCAGCAT 
TTCCAGAGGG ACCACCCGCA AAATGTGCTC CAGTTTGTAA GCAACTGGCT CTGGCGCTTC 
TACCTCCAAC CTGCTCTCCT TGGCCCAGAG TCATATGGAG TCACGGAAAA GCAAATGAGT 
CCCCTCCAGA AGCGTAACCT CGGCGAGGTT GCAAAAGTCC TGGGCCAGAT TGCATCAGGC 
AGACCATTTG GCGGAGAGAA CATCTATCTC CAGCCTCTCA ATGCTTTCGT CGCCGAAGCA 
ATCGAGCGCT TGGCTCACAT CACCAATTCC CTTATCGATG TACCTGACGC TGAGAAGACA 
TTCGACATTG ACGAGTTCAA CGATCTCTAC GCCAAGAACA AGCCAACACT GTACATCAAG 
ATGGCGGATA TCTTTTCCAT CCACAATCTC ATCGCTGCCG AGTTGCCTTA TCTGTGCCCG 
AGCAGAGATG ATATGCTGCG CGAGATCATT CAAGATCTCG GTAACGCCAA GAATAATGAG 
AATGAGATGA CCGCAGGCGG GGCCGCTGAT ATTCAGCTGT TCCTGACTCC CAAACTGCAC 
GATATGGAGG ATCCAGAAGC TGAGACTAAG GGGCTGTTTA TGGAGACCAA GAGGTGCATT 
CTGTACATCA TCCGGGTGCA AACGGGTAAC AACCTGCTTG AGATTCTTGT CAAGCCAATT 
ACCCCGGAGG ATGAGCAGAA GTGGAGGACA GTTCTGCGCG AAGACTTTGG TGACGACTCG 
AACACCCGAG GCGCTTATTC GGAGGCGAAC ATGATTGATG TTACGCGCAT GAGTTACTAC 
GACCTAAAGA GGACAGCACT CGAGAACATC CTCAGGTTGG AGCAGATGGG TCGTATCTCC 
AAGCAAAACT ACTACCAGGA TATCCTCAAC GCCATTGCGC TGGACATTCG CACAAAGAGC 
AGGAGAAGGG TTCAGAGACA GAGAGAGCTC GAGGGTGTCC GTCAGACACT CGGCAATCTT 
CACGAGAAGG CAAAGTATCT GGAGCAACAG CGCAAGAGCT ACGATGATTA CATCGAGCAA 
GCCATGGCTA CGCTGCAGAA CAAGAAGGGC AAGAAACGTT TCCTTCTCCC CTTCACCAAG 
CAATACAACC ACCAGCGCGA GCTCGAGCGC TCCGGCCGCG TCCCCAAGTT CGGCTCCTTC 
AAGTACTCGG CCCGCGCCCT CTCGGACAAG GGCGTGCTGG TCTCGTGGAC GGGCATCCCC 
GAGCGCGACT GGGGTCAAAT CAACATCACC ATCTCGTGCG ACGAGGTCGG CATTTTTAGT 
CTTGAGGGCA GCCGCGGCCA CATCCAGATG CCCGGTGCCA GCGCCCTGGT GCCCATCGAG 
GACTTGCTAC AGGCGCAATT TGAGGCGCAT CAGTTTATGA ATTTGTTTGA GGGGCAGCTG 
AGGCTGAACG TGAATTTGTT GTTGCATTTG TTGTACAAGA AGTTCTATCG GACTCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NCU03116 ( XM_011394785.1 ) cDNA ORF clone, Neurospora crassa OR74A -> XP_011393087.1 Neurospora crassa OR74A Ras GTPase activating protein, variant 3 (NCU03116), mRNA.

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CloneID	ONf13025
Clone ID Related Accession (Same CDS sequence)	XM_011394782.1 , XM_011394785.1 , XM_011394783.1 , XM_011394784.1
Accession Version	XM_011394785.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5400bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-10
Organism	Neurospora crassa OR74A
Product	Ras GTPase activating protein, variant 3
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026501).

ATGTCGTCGT ACAAACCACA CCCCCTTCGT CAGACACAGA CCGCCGACTA CTCGGTTAAT 
AGACAATCCT CCACCACATC AACATCATCA TCAGGGTACT CGGCCTCTTC CATAGGACAG 
TCGGATATCA GTCGACCCAT TAGCTCCTTG TCTACAGCAA CCAGCGTCTA CTCCCAATAT 
GGACACAAGC GGGGCCAAAG CGAGGCGAGC CGACTACCCC CAAGCCTGTC TGGTGGCTCA 
CACACCCTCC CGTCCACGCC GACAAGTGAC AATATCTACA AGAATGCCCG CCAGTCCCTC 
CGACCGACAT CTCAAGCCTC TACGGGAGCA GCGCCCGCCA GCAGCAGCAT ATCGACTGCG 
GGTTCGAGAA TCGCGCCGCG CACGCCCACA CCAGGCCACC ACGATCGTGG AAAAAGTGTT 
GACATCAGCA AGCTGTCCAT CGGAGAGTAT GGGACCTCGA CTACCCATCG CGAACGCGGT 
CAGAGTCTCG ACAACGGCAA GCCTCTGGCG ACTGAGCGCC CACGGATATC GCCTACGGGA 
ACTGTTCATG AACGTCTAGC AAGCTTCGAT GTCAGCAAGT CCTCGGTACC GGAACGTCCG 
CGGATATCGC CTACGGGTGC CCTCCACGAG CGCGGTCACA CTATCGATAT GGGCAAATTA 
TCGATTAGCG ATAATACACG TACTTCGTCA GGCATGTCTC CGACCGTGAC ATCGCCTCCG 
GCAACTTCTC CAACGACCCC TTCAACGCGA AGGCCGGCTT CCATGTTTCT ATCCAGATCA 
GATTCTGTGC GTGGCGGAAG TGACAATGGG TCAATGGCTC TGGTACACTC GAGTCCCATT 
GCCCTTGCTC GTCCGGATCT GGAAAAATTA GGTCGTTCAT CGACAAGCCA ACTCCGAACA 
CTTTCTCAGT TCGCACAAGA TGGAACGGCG GGAGAGCTTG CCATCATATC TCCGTCGCAG 
GAAGTTGTTG GTCTTCGCGG ACGCCGTAGG TTGCAAAGAG CTGACAAGTC TGTAGCGGCG 
CGTCAAAAGA ATGGCGGCTT TGGTTGGGAG ACGAAGTGGA TGGACAAGCA ACGGCAGTTT 
CTACAGGCTT ATGAATACCT TTGCCACATT GGTGAAGCGA AGGAATGGAT TGAGGATATC 
ATACAGCGTC CGATCCCCCC GATTATCGAA TTAGAGGAGG CTTTGCGAGA TGGAGTTACT 
TTGGCCGAAG TGGTCGAATC CCTTAACCCT GAAAAGAAGT TCCGCATTTT CCGCCATCCT 
AAGCTTCAAT ACCGCCATTC AGACAATATC GCCATCTTCT TCCGTTACCT TGATGAAGTC 
GAACTTCCCG ACCTCTTCCG ATTTGAGCTT ATCGATCTGT ACGAGAAAAA GAACATCCCG 
AAAGTTATTT ACTGTCTCCA TGCTTTGAGT TGGCTTCTCT TCCGGAAAGG AATTGTCGAT 
TTCCGCATCG GGAATCTGGT CGGCCAGCTT GAGTTTGAAG ACCATGAGCT GGAGGCGATG 
CAGAAAGGCC TCGATAAGCT GGGGGCTAGT ATGCCTAGCT TCGGCAATAT GGGTGCCGAC 
TTTGGAATTG AGCCAGCTGA ACCCGAGGAG ACGGAGGAGG AACGCATTGA GAGGGAACTG 
GCCGAAAACG AAGAGGCCAT TGTAGAGTTA CAAGCCCAGG CCAGAGCGGC GCTTCAAAGG 
ATACAGCTTG GCTACACTAT GCAACATCTG TGGGATAATG AAGACTGGAT CATTGATCTC 
CAGTCTCGTA TTCGTGGTGA CTGGGCTCGC CAGGTGGCGG ATTACAGGCT CCAGATGAAG 
AATTTCGCGG TCCAGCTTCA AAGCGCAGCG CGCGGTTTCC TCGCCCGCTA TCGACATGCA 
GAAACAAAGG CACAAACAAG TTTTGCGATC GAGTTGCAGA AGACAGCTCG TGGATTTATC 
GCTCGTCAGC AATATGCACA AACATGTTTC ACGATTCAGC TACAAAAAGC GGCGCGTGGA 
TATCTTGCTC GTCGCCAGCA GACACAAACC GAGGAGCAGT TCAAGGGCAA CGAGGATGTG 
GTCTTGAAAC TCCAAAGTAT GGTCAGAGCG GCCAAGGTCC GCAATGTGAT TCGGGAGCAG 
CATTCACAGT TGGAGACGGC AGCGGGACCC CTGCGAGACA TTCAAGCAAC ATTTCGAGGA 
TTTCTGGCTC GAAAGCAGCT CGCTGTGCAA CATGAAAAGA CAGAGAGCGA AGGCGCTACG 
ATTGCTCAAC TTCAGAGCGC AGTTCGTGGT ATGATATTGA GGAAGAATCT GGAGGAAGAA 
AGAGCATCCT TGGAGAGCAA CATCGAAGCC ATCACTAGTC TTCAGGCTGC TGTAAGGGCC 
ATGAAAGCAA GAACTGAGGT CGATGCCCAG CTACAAGCGC TTGAAGGCTT TGCGCCACAA 
TGGACAGCTA TTCAAGCTGC CTTCCGCGGC AAGGTTGCTA GGTCCGACGT CGACGCTACT 
AAAGAAGAGT TGCAGAAGCA TTCAACTGAG ATGGGCCTTC TTCAGTCCCA CATTAGGGCA 
GCGGCAGTGC GGAGGGACAT ATCAGAGCAG CTTGAAAGCA TCGGGGAAAC GGAGGATGCC 
ATCATTGATC TTCAGTCTAT GGTCCGAGGT ATGCTTGAAC GTATGAGGTT ATCGGACGAT 
CTGGATGCTC TTACTCAGGA GACACCAAAC ATCATTGACC TTCAAGCACA CATCCGCGGA 
TGTCTTTATC GCAAGGAGTG TGGCGAGTTG TTGGATGAGC TTGCAGTCAA TACCGAGGAG 
ATCACCGAAC TGCAAGCCCT TGCTCGGGCC ATGTTGCTTC GGAGCGATGT TGCAATGGTC 
TTGGATGAAC TGGAACAAAA CGAGGAGTCT GTAGCGGCGT TCCAGGCTGC TGCCAAGGCT 
TTCGTGGTCA GAGCCAAGTA CGAGGAGAAG AAGCGCTTCT TCCGGGAGAA CATGCAAAAG 
GTTGTCAAGA TTCAGAGTTT CGTACGAGCC AAGCTTCAGG GTGAAGCCTA CAAGAGCTTG 
ACATCTGGAA AGAACCCACC AGTCAACGCC GTCAAGAACT TTGTCCATCT TCTGAACGAC 
AGCGATTTTG ACTTCAATGA GGAGGTAGAG TTTGAGCGCC TACGAAAGAC TGTTGTCCAA 
CAAGTTCGGC AAAACGAGAT GCTGGAACAA TACATCGACC AGCTAGACAT CAAGATCGCA 
CTTCTCGTCA AGAATAAAAT TACCCTTGAT GAAGTTGTCA GACATCAGCA CAACTATGGC 
GGACACGCAA ACAGCCTTCT CGCCAACAGT GTTATTTCAA ATGCTAATCA GTTTGACCTC 
AAGGCTTTGA ATAAGAGTTC CCGCAAGAAG CTTGAGTCCT ACCAGCAGCT GTTCTTCACC 
CTTCAGACGC AACCTCAGTA CCTTGCTAAG CTCTTCAGGC GTATCCGCGA GCAAGGAATA 
ACGGAAAAGG AGTTAAAGCG GATTGAACAC TTGATGATGG GGCTGTTCGG CTATGCGCAA 
AAGCGGAGAG AGGAATACTA CTTATTGAAG CTTATTTCCA GGTCGATAAG GGAAGAAGTC 
GAAGCATGTG CCGCTCTGCA GGATTATCTG AGGGGCAACT ACTTCTGGAC CAAGCTGCTC 
CAGAATTACA CCCGATCCCC TCGTGATAGG AAATATCTCA GAGATCTCTT GGGACCCCTC 
ATCAGAGACA ACATTATCGA GGATCCAGCG CTGGATCTTG AAAGCGACCC CATGCAGATC 
TACCGGTCTG CTATCAACAA TGAGGAGTTG AGGACCGGTC GGCCCAGCCA AAGACCTCTG 
GATGTCCCTC GTGAGGTTGC CATCAAGGAT CCTGAAACCA GAGAGCTCTT CATTGATCAT 
CTGCGGGATC TCCGAGAGAT CTGCGATCAG TTTCTCCTTG CCCTGGAGGA CCTGCTTCCC 
CGCATGCCCT ACGGCCTGAG ATTTGTGTGC CGGCAAACGT ACGAAGCTCT CTGCCAGCAT 
TTCCAGAGGG ACCACCCGCA AAATGTGCTC CAGTTTGTAA GCAACTGGCT CTGGCGCTTC 
TACCTCCAAC CTGCTCTCCT TGGCCCAGAG TCATATGGAG TCACGGAAAA GCAAATGAGT 
CCCCTCCAGA AGCGTAACCT CGGCGAGGTT GCAAAAGTCC TGGGCCAGAT TGCATCAGGC 
AGACCATTTG GCGGAGAGAA CATCTATCTC CAGCCTCTCA ATGCTTTCGT CGCCGAAGCA 
ATCGAGCGCT TGGCTCACAT CACCAATTCC CTTATCGATG TACCTGACGC TGAGAAGACA 
TTCGACATTG ACGAGTTCAA CGATCTCTAC GCCAAGAACA AGCCAACACT GTACATCAAG 
ATGGCGGATA TCTTTTCCAT CCACAATCTC ATCGCTGCCG AGTTGCCTTA TCTGTGCCCG 
AGCAGAGATG ATATGCTGCG CGAGATCATT CAAGATCTCG GTAACGCCAA GAATAATGAG 
AATGAGATGA CCGCAGGCGG GGCCGCTGAT ATTCAGCTGT TCCTGACTCC CAAACTGCAC 
GATATGGAGG ATCCAGAAGC TGAGACTAAG GGGCTGTTTA TGGAGACCAA GAGGTGCATT 
CTGTACATCA TCCGGGTGCA AACGGGTAAC AACCTGCTTG AGATTCTTGT CAAGCCAATT 
ACCCCGGAGG ATGAGCAGAA GTGGAGGACA GTTCTGCGCG AAGACTTTGG TGACGACTCG 
AACACCCGAG GCGCTTATTC GGAGGCGAAC ATGATTGATG TTACGCGCAT GAGTTACTAC 
GACCTAAAGA GGACAGCACT CGAGAACATC CTCAGGTTGG AGCAGATGGG TCGTATCTCC 
AAGCAAAACT ACTACCAGGA TATCCTCAAC GCCATTGCGC TGGACATTCG CACAAAGAGC 
AGGAGAAGGG TTCAGAGACA GAGAGAGCTC GAGGGTGTCC GTCAGACACT CGGCAATCTT 
CACGAGAAGG CAAAGTATCT GGAGCAACAG CGCAAGAGCT ACGATGATTA CATCGAGCAA 
GCCATGGCTA CGCTGCAGAA CAAGAAGGGC AAGAAACGTT TCCTTCTCCC CTTCACCAAG 
CAATACAACC ACCAGCGCGA GCTCGAGCGC TCCGGCCGCG TCCCCAAGTT CGGCTCCTTC 
AAGTACTCGG CCCGCGCCCT CTCGGACAAG GGCGTGCTGG TCTCGTGGAC GGGCATCCCC 
GAGCGCGACT GGGGTCAAAT CAACATCACC ATCTCGTGCG ACGAGGTCGG CATTTTTAGT 
CTTGAGGGCA GCCGCGGCCA CATCCAGATG CCCGGTGCCA GCGCCCTGGT GCCCATCGAG 
GACTTGCTAC AGGCGCAATT TGAGGCGCAT CAGTTTATGA ATTTGTTTGA GGGGCAGCTG 
AGGCTGAACG TGAATTTGTT GTTGCATTTG TTGTACAAGA AGTTCTATCG GACTCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011393087.1
CDS	244..5643
Translation	MSSYKPHPLRQTQTADYSVNRQSSTTSTSSSGYSASSIGQSDISRPISSLSTATSVYSQYGHKRGQSEASRLPPSLSGGSHTLPSTPTSDNIYKNARQSLRPTSQASTGAAPASSSISTAGSRIAPRTPTPGHHDRGKSVDISKLSIGEYGTSTTHRERGQSLDNGKPLATERPRISPTGTVHERLASFDVSKSSVPERPRISPTGALHERGHTIDMGKLSISDNTRTSSGMSPTVTSPPATSPTTPSTRRPASMFLSRSDSVRGGSDNGSMALVHSSPIALARPDLEKLGRSSTSQLRTLSQFAQDGTAGELAIISPSQEVVGLRGRRRLQRADKSVAARQKNGGFGWETKWMDKQRQFLQAYEYLCHIGEAKEWIEDIIQRPIPPIIELEEALRDGVTLAEVVESLNPEKKFRIFRHPKLQYRHSDNIAIFFRYLDEVELPDLFRFELIDLYEKKNIPKVIYCLHALSWLLFRKGIVDFRIGNLVGQLEFEDHELEAMQKGLDKLGASMPSFGNMGADFGIEPAEPEETEEERIERELAENEEAIVELQAQARAALQRIQLGYTMQHLWDNEDWIIDLQSRIRGDWARQVADYRLQMKNFAVQLQSAARGFLARYRHAETKAQTSFAIELQKTARGFIARQQYAQTCFTIQLQKAARGYLARRQQTQTEEQFKGNEDVVLKLQSMVRAAKVRNVIREQHSQLETAAGPLRDIQATFRGFLARKQLAVQHEKTESEGATIAQLQSAVRGMILRKNLEEERASLESNIEAITSLQAAVRAMKARTEVDAQLQALEGFAPQWTAIQAAFRGKVARSDVDATKEELQKHSTEMGLLQSHIRAAAVRRDISEQLESIGETEDAIIDLQSMVRGMLERMRLSDDLDALTQETPNIIDLQAHIRGCLYRKECGELLDELAVNTEEITELQALARAMLLRSDVAMVLDELEQNEESVAAFQAAAKAFVVRAKYEEKKRFFRENMQKVVKIQSFVRAKLQGEAYKSLTSGKNPPVNAVKNFVHLLNDSDFDFNEEVEFERLRKTVVQQVRQNEMLEQYIDQLDIKIALLVKNKITLDEVVRHQHNYGGHANSLLANSVISNANQFDLKALNKSSRKKLESYQQLFFTLQTQPQYLAKLFRRIREQGITEKELKRIEHLMMGLFGYAQKRREEYYLLKLISRSIREEVEACAALQDYLRGNYFWTKLLQNYTRSPRDRKYLRDLLGPLIRDNIIEDPALDLESDPMQIYRSAINNEELRTGRPSQRPLDVPREVAIKDPETRELFIDHLRDLREICDQFLLALEDLLPRMPYGLRFVCRQTYEALCQHFQRDHPQNVLQFVSNWLWRFYLQPALLGPESYGVTEKQMSPLQKRNLGEVAKVLGQIASGRPFGGENIYLQPLNAFVAEAIERLAHITNSLIDVPDAEKTFDIDEFNDLYAKNKPTLYIKMADIFSIHNLIAAELPYLCPSRDDMLREIIQDLGNAKNNENEMTAGGAADIQLFLTPKLHDMEDPEAETKGLFMETKRCILYIIRVQTGNNLLEILVKPITPEDEQKWRTVLREDFGDDSNTRGAYSEANMIDVTRMSYYDLKRTALENILRLEQMGRISKQNYYQDILNAIALDIRTKSRRRVQRQRELEGVRQTLGNLHEKAKYLEQQRKSYDDYIEQAMATLQNKKGKKRFLLPFTKQYNHQRELERSGRVPKFGSFKYSARALSDKGVLVSWTGIPERDWGQINITISCDEVGIFSLEGSRGHIQMPGASALVPIEDLLQAQFEAHQFMNLFEGQLRLNVNLLLHLLYKKFYRTQ

Target ORF information:

RefSeq Version	XM_011394785.1
Organism	Neurospora crassa OR74A
Definition	Neurospora crassa OR74A Ras GTPase activating protein, variant 3 (NCU03116), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011394785.1

ORF Insert Sequence:

ATGTCGTCGT ACAAACCACA CCCCCTTCGT CAGACACAGA CCGCCGACTA CTCGGTTAAT 
AGACAATCCT CCACCACATC AACATCATCA TCAGGGTACT CGGCCTCTTC CATAGGACAG 
TCGGATATCA GTCGACCCAT TAGCTCCTTG TCTACAGCAA CCAGCGTCTA CTCCCAATAT 
GGACACAAGC GGGGCCAAAG CGAGGCGAGC CGACTACCCC CAAGCCTGTC TGGTGGCTCA 
CACACCCTCC CGTCCACGCC GACAAGTGAC AATATCTACA AGAATGCCCG CCAGTCCCTC 
CGACCGACAT CTCAAGCCTC TACGGGAGCA GCGCCCGCCA GCAGCAGCAT ATCGACTGCG 
GGTTCGAGAA TCGCGCCGCG CACGCCCACA CCAGGCCACC ACGATCGTGG AAAAAGTGTT 
GACATCAGCA AGCTGTCCAT CGGAGAGTAT GGGACCTCGA CTACCCATCG CGAACGCGGT 
CAGAGTCTCG ACAACGGCAA GCCTCTGGCG ACTGAGCGCC CACGGATATC GCCTACGGGA 
ACTGTTCATG AACGTCTAGC AAGCTTCGAT GTCAGCAAGT CCTCGGTACC GGAACGTCCG 
CGGATATCGC CTACGGGTGC CCTCCACGAG CGCGGTCACA CTATCGATAT GGGCAAATTA 
TCGATTAGCG ATAATACACG TACTTCGTCA GGCATGTCTC CGACCGTGAC ATCGCCTCCG 
GCAACTTCTC CAACGACCCC TTCAACGCGA AGGCCGGCTT CCATGTTTCT ATCCAGATCA 
GATTCTGTGC GTGGCGGAAG TGACAATGGG TCAATGGCTC TGGTACACTC GAGTCCCATT 
GCCCTTGCTC GTCCGGATCT GGAAAAATTA GGTCGTTCAT CGACAAGCCA ACTCCGAACA 
CTTTCTCAGT TCGCACAAGA TGGAACGGCG GGAGAGCTTG CCATCATATC TCCGTCGCAG 
GAAGTTGTTG GTCTTCGCGG ACGCCGTAGG TTGCAAAGAG CTGACAAGTC TGTAGCGGCG 
CGTCAAAAGA ATGGCGGCTT TGGTTGGGAG ACGAAGTGGA TGGACAAGCA ACGGCAGTTT 
CTACAGGCTT ATGAATACCT TTGCCACATT GGTGAAGCGA AGGAATGGAT TGAGGATATC 
ATACAGCGTC CGATCCCCCC GATTATCGAA TTAGAGGAGG CTTTGCGAGA TGGAGTTACT 
TTGGCCGAAG TGGTCGAATC CCTTAACCCT GAAAAGAAGT TCCGCATTTT CCGCCATCCT 
AAGCTTCAAT ACCGCCATTC AGACAATATC GCCATCTTCT TCCGTTACCT TGATGAAGTC 
GAACTTCCCG ACCTCTTCCG ATTTGAGCTT ATCGATCTGT ACGAGAAAAA GAACATCCCG 
AAAGTTATTT ACTGTCTCCA TGCTTTGAGT TGGCTTCTCT TCCGGAAAGG AATTGTCGAT 
TTCCGCATCG GGAATCTGGT CGGCCAGCTT GAGTTTGAAG ACCATGAGCT GGAGGCGATG 
CAGAAAGGCC TCGATAAGCT GGGGGCTAGT ATGCCTAGCT TCGGCAATAT GGGTGCCGAC 
TTTGGAATTG AGCCAGCTGA ACCCGAGGAG ACGGAGGAGG AACGCATTGA GAGGGAACTG 
GCCGAAAACG AAGAGGCCAT TGTAGAGTTA CAAGCCCAGG CCAGAGCGGC GCTTCAAAGG 
ATACAGCTTG GCTACACTAT GCAACATCTG TGGGATAATG AAGACTGGAT CATTGATCTC 
CAGTCTCGTA TTCGTGGTGA CTGGGCTCGC CAGGTGGCGG ATTACAGGCT CCAGATGAAG 
AATTTCGCGG TCCAGCTTCA AAGCGCAGCG CGCGGTTTCC TCGCCCGCTA TCGACATGCA 
GAAACAAAGG CACAAACAAG TTTTGCGATC GAGTTGCAGA AGACAGCTCG TGGATTTATC 
GCTCGTCAGC AATATGCACA AACATGTTTC ACGATTCAGC TACAAAAAGC GGCGCGTGGA 
TATCTTGCTC GTCGCCAGCA GACACAAACC GAGGAGCAGT TCAAGGGCAA CGAGGATGTG 
GTCTTGAAAC TCCAAAGTAT GGTCAGAGCG GCCAAGGTCC GCAATGTGAT TCGGGAGCAG 
CATTCACAGT TGGAGACGGC AGCGGGACCC CTGCGAGACA TTCAAGCAAC ATTTCGAGGA 
TTTCTGGCTC GAAAGCAGCT CGCTGTGCAA CATGAAAAGA CAGAGAGCGA AGGCGCTACG 
ATTGCTCAAC TTCAGAGCGC AGTTCGTGGT ATGATATTGA GGAAGAATCT GGAGGAAGAA 
AGAGCATCCT TGGAGAGCAA CATCGAAGCC ATCACTAGTC TTCAGGCTGC TGTAAGGGCC 
ATGAAAGCAA GAACTGAGGT CGATGCCCAG CTACAAGCGC TTGAAGGCTT TGCGCCACAA 
TGGACAGCTA TTCAAGCTGC CTTCCGCGGC AAGGTTGCTA GGTCCGACGT CGACGCTACT 
AAAGAAGAGT TGCAGAAGCA TTCAACTGAG ATGGGCCTTC TTCAGTCCCA CATTAGGGCA 
GCGGCAGTGC GGAGGGACAT ATCAGAGCAG CTTGAAAGCA TCGGGGAAAC GGAGGATGCC 
ATCATTGATC TTCAGTCTAT GGTCCGAGGT ATGCTTGAAC GTATGAGGTT ATCGGACGAT 
CTGGATGCTC TTACTCAGGA GACACCAAAC ATCATTGACC TTCAAGCACA CATCCGCGGA 
TGTCTTTATC GCAAGGAGTG TGGCGAGTTG TTGGATGAGC TTGCAGTCAA TACCGAGGAG 
ATCACCGAAC TGCAAGCCCT TGCTCGGGCC ATGTTGCTTC GGAGCGATGT TGCAATGGTC 
TTGGATGAAC TGGAACAAAA CGAGGAGTCT GTAGCGGCGT TCCAGGCTGC TGCCAAGGCT 
TTCGTGGTCA GAGCCAAGTA CGAGGAGAAG AAGCGCTTCT TCCGGGAGAA CATGCAAAAG 
GTTGTCAAGA TTCAGAGTTT CGTACGAGCC AAGCTTCAGG GTGAAGCCTA CAAGAGCTTG 
ACATCTGGAA AGAACCCACC AGTCAACGCC GTCAAGAACT TTGTCCATCT TCTGAACGAC 
AGCGATTTTG ACTTCAATGA GGAGGTAGAG TTTGAGCGCC TACGAAAGAC TGTTGTCCAA 
CAAGTTCGGC AAAACGAGAT GCTGGAACAA TACATCGACC AGCTAGACAT CAAGATCGCA 
CTTCTCGTCA AGAATAAAAT TACCCTTGAT GAAGTTGTCA GACATCAGCA CAACTATGGC 
GGACACGCAA ACAGCCTTCT CGCCAACAGT GTTATTTCAA ATGCTAATCA GTTTGACCTC 
AAGGCTTTGA ATAAGAGTTC CCGCAAGAAG CTTGAGTCCT ACCAGCAGCT GTTCTTCACC 
CTTCAGACGC AACCTCAGTA CCTTGCTAAG CTCTTCAGGC GTATCCGCGA GCAAGGAATA 
ACGGAAAAGG AGTTAAAGCG GATTGAACAC TTGATGATGG GGCTGTTCGG CTATGCGCAA 
AAGCGGAGAG AGGAATACTA CTTATTGAAG CTTATTTCCA GGTCGATAAG GGAAGAAGTC 
GAAGCATGTG CCGCTCTGCA GGATTATCTG AGGGGCAACT ACTTCTGGAC CAAGCTGCTC 
CAGAATTACA CCCGATCCCC TCGTGATAGG AAATATCTCA GAGATCTCTT GGGACCCCTC 
ATCAGAGACA ACATTATCGA GGATCCAGCG CTGGATCTTG AAAGCGACCC CATGCAGATC 
TACCGGTCTG CTATCAACAA TGAGGAGTTG AGGACCGGTC GGCCCAGCCA AAGACCTCTG 
GATGTCCCTC GTGAGGTTGC CATCAAGGAT CCTGAAACCA GAGAGCTCTT CATTGATCAT 
CTGCGGGATC TCCGAGAGAT CTGCGATCAG TTTCTCCTTG CCCTGGAGGA CCTGCTTCCC 
CGCATGCCCT ACGGCCTGAG ATTTGTGTGC CGGCAAACGT ACGAAGCTCT CTGCCAGCAT 
TTCCAGAGGG ACCACCCGCA AAATGTGCTC CAGTTTGTAA GCAACTGGCT CTGGCGCTTC 
TACCTCCAAC CTGCTCTCCT TGGCCCAGAG TCATATGGAG TCACGGAAAA GCAAATGAGT 
CCCCTCCAGA AGCGTAACCT CGGCGAGGTT GCAAAAGTCC TGGGCCAGAT TGCATCAGGC 
AGACCATTTG GCGGAGAGAA CATCTATCTC CAGCCTCTCA ATGCTTTCGT CGCCGAAGCA 
ATCGAGCGCT TGGCTCACAT CACCAATTCC CTTATCGATG TACCTGACGC TGAGAAGACA 
TTCGACATTG ACGAGTTCAA CGATCTCTAC GCCAAGAACA AGCCAACACT GTACATCAAG 
ATGGCGGATA TCTTTTCCAT CCACAATCTC ATCGCTGCCG AGTTGCCTTA TCTGTGCCCG 
AGCAGAGATG ATATGCTGCG CGAGATCATT CAAGATCTCG GTAACGCCAA GAATAATGAG 
AATGAGATGA CCGCAGGCGG GGCCGCTGAT ATTCAGCTGT TCCTGACTCC CAAACTGCAC 
GATATGGAGG ATCCAGAAGC TGAGACTAAG GGGCTGTTTA TGGAGACCAA GAGGTGCATT 
CTGTACATCA TCCGGGTGCA AACGGGTAAC AACCTGCTTG AGATTCTTGT CAAGCCAATT 
ACCCCGGAGG ATGAGCAGAA GTGGAGGACA GTTCTGCGCG AAGACTTTGG TGACGACTCG 
AACACCCGAG GCGCTTATTC GGAGGCGAAC ATGATTGATG TTACGCGCAT GAGTTACTAC 
GACCTAAAGA GGACAGCACT CGAGAACATC CTCAGGTTGG AGCAGATGGG TCGTATCTCC 
AAGCAAAACT ACTACCAGGA TATCCTCAAC GCCATTGCGC TGGACATTCG CACAAAGAGC 
AGGAGAAGGG TTCAGAGACA GAGAGAGCTC GAGGGTGTCC GTCAGACACT CGGCAATCTT 
CACGAGAAGG CAAAGTATCT GGAGCAACAG CGCAAGAGCT ACGATGATTA CATCGAGCAA 
GCCATGGCTA CGCTGCAGAA CAAGAAGGGC AAGAAACGTT TCCTTCTCCC CTTCACCAAG 
CAATACAACC ACCAGCGCGA GCTCGAGCGC TCCGGCCGCG TCCCCAAGTT CGGCTCCTTC 
AAGTACTCGG CCCGCGCCCT CTCGGACAAG GGCGTGCTGG TCTCGTGGAC GGGCATCCCC 
GAGCGCGACT GGGGTCAAAT CAACATCACC ATCTCGTGCG ACGAGGTCGG CATTTTTAGT 
CTTGAGGGCA GCCGCGGCCA CATCCAGATG CCCGGTGCCA GCGCCCTGGT GCCCATCGAG 
GACTTGCTAC AGGCGCAATT TGAGGCGCAT CAGTTTATGA ATTTGTTTGA GGGGCAGCTG 
AGGCTGAACG TGAATTTGTT GTTGCATTTG TTGTACAAGA AGTTCTATCG GACTCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NCU03116 ( XM_011394783.1 ) cDNA ORF clone, Neurospora crassa OR74A -> XP_011393085.1 Neurospora crassa OR74A Ras GTPase activating protein (NCU03116), mRNA.

Price & Availability↑

CloneID	ONf13025
Clone ID Related Accession (Same CDS sequence)	XM_011394782.1 , XM_011394785.1 , XM_011394783.1 , XM_011394784.1
Accession Version	XM_011394783.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5400bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-10
Organism	Neurospora crassa OR74A
Product	Ras GTPase activating protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026501).

ATGTCGTCGT ACAAACCACA CCCCCTTCGT CAGACACAGA CCGCCGACTA CTCGGTTAAT 
AGACAATCCT CCACCACATC AACATCATCA TCAGGGTACT CGGCCTCTTC CATAGGACAG 
TCGGATATCA GTCGACCCAT TAGCTCCTTG TCTACAGCAA CCAGCGTCTA CTCCCAATAT 
GGACACAAGC GGGGCCAAAG CGAGGCGAGC CGACTACCCC CAAGCCTGTC TGGTGGCTCA 
CACACCCTCC CGTCCACGCC GACAAGTGAC AATATCTACA AGAATGCCCG CCAGTCCCTC 
CGACCGACAT CTCAAGCCTC TACGGGAGCA GCGCCCGCCA GCAGCAGCAT ATCGACTGCG 
GGTTCGAGAA TCGCGCCGCG CACGCCCACA CCAGGCCACC ACGATCGTGG AAAAAGTGTT 
GACATCAGCA AGCTGTCCAT CGGAGAGTAT GGGACCTCGA CTACCCATCG CGAACGCGGT 
CAGAGTCTCG ACAACGGCAA GCCTCTGGCG ACTGAGCGCC CACGGATATC GCCTACGGGA 
ACTGTTCATG AACGTCTAGC AAGCTTCGAT GTCAGCAAGT CCTCGGTACC GGAACGTCCG 
CGGATATCGC CTACGGGTGC CCTCCACGAG CGCGGTCACA CTATCGATAT GGGCAAATTA 
TCGATTAGCG ATAATACACG TACTTCGTCA GGCATGTCTC CGACCGTGAC ATCGCCTCCG 
GCAACTTCTC CAACGACCCC TTCAACGCGA AGGCCGGCTT CCATGTTTCT ATCCAGATCA 
GATTCTGTGC GTGGCGGAAG TGACAATGGG TCAATGGCTC TGGTACACTC GAGTCCCATT 
GCCCTTGCTC GTCCGGATCT GGAAAAATTA GGTCGTTCAT CGACAAGCCA ACTCCGAACA 
CTTTCTCAGT TCGCACAAGA TGGAACGGCG GGAGAGCTTG CCATCATATC TCCGTCGCAG 
GAAGTTGTTG GTCTTCGCGG ACGCCGTAGG TTGCAAAGAG CTGACAAGTC TGTAGCGGCG 
CGTCAAAAGA ATGGCGGCTT TGGTTGGGAG ACGAAGTGGA TGGACAAGCA ACGGCAGTTT 
CTACAGGCTT ATGAATACCT TTGCCACATT GGTGAAGCGA AGGAATGGAT TGAGGATATC 
ATACAGCGTC CGATCCCCCC GATTATCGAA TTAGAGGAGG CTTTGCGAGA TGGAGTTACT 
TTGGCCGAAG TGGTCGAATC CCTTAACCCT GAAAAGAAGT TCCGCATTTT CCGCCATCCT 
AAGCTTCAAT ACCGCCATTC AGACAATATC GCCATCTTCT TCCGTTACCT TGATGAAGTC 
GAACTTCCCG ACCTCTTCCG ATTTGAGCTT ATCGATCTGT ACGAGAAAAA GAACATCCCG 
AAAGTTATTT ACTGTCTCCA TGCTTTGAGT TGGCTTCTCT TCCGGAAAGG AATTGTCGAT 
TTCCGCATCG GGAATCTGGT CGGCCAGCTT GAGTTTGAAG ACCATGAGCT GGAGGCGATG 
CAGAAAGGCC TCGATAAGCT GGGGGCTAGT ATGCCTAGCT TCGGCAATAT GGGTGCCGAC 
TTTGGAATTG AGCCAGCTGA ACCCGAGGAG ACGGAGGAGG AACGCATTGA GAGGGAACTG 
GCCGAAAACG AAGAGGCCAT TGTAGAGTTA CAAGCCCAGG CCAGAGCGGC GCTTCAAAGG 
ATACAGCTTG GCTACACTAT GCAACATCTG TGGGATAATG AAGACTGGAT CATTGATCTC 
CAGTCTCGTA TTCGTGGTGA CTGGGCTCGC CAGGTGGCGG ATTACAGGCT CCAGATGAAG 
AATTTCGCGG TCCAGCTTCA AAGCGCAGCG CGCGGTTTCC TCGCCCGCTA TCGACATGCA 
GAAACAAAGG CACAAACAAG TTTTGCGATC GAGTTGCAGA AGACAGCTCG TGGATTTATC 
GCTCGTCAGC AATATGCACA AACATGTTTC ACGATTCAGC TACAAAAAGC GGCGCGTGGA 
TATCTTGCTC GTCGCCAGCA GACACAAACC GAGGAGCAGT TCAAGGGCAA CGAGGATGTG 
GTCTTGAAAC TCCAAAGTAT GGTCAGAGCG GCCAAGGTCC GCAATGTGAT TCGGGAGCAG 
CATTCACAGT TGGAGACGGC AGCGGGACCC CTGCGAGACA TTCAAGCAAC ATTTCGAGGA 
TTTCTGGCTC GAAAGCAGCT CGCTGTGCAA CATGAAAAGA CAGAGAGCGA AGGCGCTACG 
ATTGCTCAAC TTCAGAGCGC AGTTCGTGGT ATGATATTGA GGAAGAATCT GGAGGAAGAA 
AGAGCATCCT TGGAGAGCAA CATCGAAGCC ATCACTAGTC TTCAGGCTGC TGTAAGGGCC 
ATGAAAGCAA GAACTGAGGT CGATGCCCAG CTACAAGCGC TTGAAGGCTT TGCGCCACAA 
TGGACAGCTA TTCAAGCTGC CTTCCGCGGC AAGGTTGCTA GGTCCGACGT CGACGCTACT 
AAAGAAGAGT TGCAGAAGCA TTCAACTGAG ATGGGCCTTC TTCAGTCCCA CATTAGGGCA 
GCGGCAGTGC GGAGGGACAT ATCAGAGCAG CTTGAAAGCA TCGGGGAAAC GGAGGATGCC 
ATCATTGATC TTCAGTCTAT GGTCCGAGGT ATGCTTGAAC GTATGAGGTT ATCGGACGAT 
CTGGATGCTC TTACTCAGGA GACACCAAAC ATCATTGACC TTCAAGCACA CATCCGCGGA 
TGTCTTTATC GCAAGGAGTG TGGCGAGTTG TTGGATGAGC TTGCAGTCAA TACCGAGGAG 
ATCACCGAAC TGCAAGCCCT TGCTCGGGCC ATGTTGCTTC GGAGCGATGT TGCAATGGTC 
TTGGATGAAC TGGAACAAAA CGAGGAGTCT GTAGCGGCGT TCCAGGCTGC TGCCAAGGCT 
TTCGTGGTCA GAGCCAAGTA CGAGGAGAAG AAGCGCTTCT TCCGGGAGAA CATGCAAAAG 
GTTGTCAAGA TTCAGAGTTT CGTACGAGCC AAGCTTCAGG GTGAAGCCTA CAAGAGCTTG 
ACATCTGGAA AGAACCCACC AGTCAACGCC GTCAAGAACT TTGTCCATCT TCTGAACGAC 
AGCGATTTTG ACTTCAATGA GGAGGTAGAG TTTGAGCGCC TACGAAAGAC TGTTGTCCAA 
CAAGTTCGGC AAAACGAGAT GCTGGAACAA TACATCGACC AGCTAGACAT CAAGATCGCA 
CTTCTCGTCA AGAATAAAAT TACCCTTGAT GAAGTTGTCA GACATCAGCA CAACTATGGC 
GGACACGCAA ACAGCCTTCT CGCCAACAGT GTTATTTCAA ATGCTAATCA GTTTGACCTC 
AAGGCTTTGA ATAAGAGTTC CCGCAAGAAG CTTGAGTCCT ACCAGCAGCT GTTCTTCACC 
CTTCAGACGC AACCTCAGTA CCTTGCTAAG CTCTTCAGGC GTATCCGCGA GCAAGGAATA 
ACGGAAAAGG AGTTAAAGCG GATTGAACAC TTGATGATGG GGCTGTTCGG CTATGCGCAA 
AAGCGGAGAG AGGAATACTA CTTATTGAAG CTTATTTCCA GGTCGATAAG GGAAGAAGTC 
GAAGCATGTG CCGCTCTGCA GGATTATCTG AGGGGCAACT ACTTCTGGAC CAAGCTGCTC 
CAGAATTACA CCCGATCCCC TCGTGATAGG AAATATCTCA GAGATCTCTT GGGACCCCTC 
ATCAGAGACA ACATTATCGA GGATCCAGCG CTGGATCTTG AAAGCGACCC CATGCAGATC 
TACCGGTCTG CTATCAACAA TGAGGAGTTG AGGACCGGTC GGCCCAGCCA AAGACCTCTG 
GATGTCCCTC GTGAGGTTGC CATCAAGGAT CCTGAAACCA GAGAGCTCTT CATTGATCAT 
CTGCGGGATC TCCGAGAGAT CTGCGATCAG TTTCTCCTTG CCCTGGAGGA CCTGCTTCCC 
CGCATGCCCT ACGGCCTGAG ATTTGTGTGC CGGCAAACGT ACGAAGCTCT CTGCCAGCAT 
TTCCAGAGGG ACCACCCGCA AAATGTGCTC CAGTTTGTAA GCAACTGGCT CTGGCGCTTC 
TACCTCCAAC CTGCTCTCCT TGGCCCAGAG TCATATGGAG TCACGGAAAA GCAAATGAGT 
CCCCTCCAGA AGCGTAACCT CGGCGAGGTT GCAAAAGTCC TGGGCCAGAT TGCATCAGGC 
AGACCATTTG GCGGAGAGAA CATCTATCTC CAGCCTCTCA ATGCTTTCGT CGCCGAAGCA 
ATCGAGCGCT TGGCTCACAT CACCAATTCC CTTATCGATG TACCTGACGC TGAGAAGACA 
TTCGACATTG ACGAGTTCAA CGATCTCTAC GCCAAGAACA AGCCAACACT GTACATCAAG 
ATGGCGGATA TCTTTTCCAT CCACAATCTC ATCGCTGCCG AGTTGCCTTA TCTGTGCCCG 
AGCAGAGATG ATATGCTGCG CGAGATCATT CAAGATCTCG GTAACGCCAA GAATAATGAG 
AATGAGATGA CCGCAGGCGG GGCCGCTGAT ATTCAGCTGT TCCTGACTCC CAAACTGCAC 
GATATGGAGG ATCCAGAAGC TGAGACTAAG GGGCTGTTTA TGGAGACCAA GAGGTGCATT 
CTGTACATCA TCCGGGTGCA AACGGGTAAC AACCTGCTTG AGATTCTTGT CAAGCCAATT 
ACCCCGGAGG ATGAGCAGAA GTGGAGGACA GTTCTGCGCG AAGACTTTGG TGACGACTCG 
AACACCCGAG GCGCTTATTC GGAGGCGAAC ATGATTGATG TTACGCGCAT GAGTTACTAC 
GACCTAAAGA GGACAGCACT CGAGAACATC CTCAGGTTGG AGCAGATGGG TCGTATCTCC 
AAGCAAAACT ACTACCAGGA TATCCTCAAC GCCATTGCGC TGGACATTCG CACAAAGAGC 
AGGAGAAGGG TTCAGAGACA GAGAGAGCTC GAGGGTGTCC GTCAGACACT CGGCAATCTT 
CACGAGAAGG CAAAGTATCT GGAGCAACAG CGCAAGAGCT ACGATGATTA CATCGAGCAA 
GCCATGGCTA CGCTGCAGAA CAAGAAGGGC AAGAAACGTT TCCTTCTCCC CTTCACCAAG 
CAATACAACC ACCAGCGCGA GCTCGAGCGC TCCGGCCGCG TCCCCAAGTT CGGCTCCTTC 
AAGTACTCGG CCCGCGCCCT CTCGGACAAG GGCGTGCTGG TCTCGTGGAC GGGCATCCCC 
GAGCGCGACT GGGGTCAAAT CAACATCACC ATCTCGTGCG ACGAGGTCGG CATTTTTAGT 
CTTGAGGGCA GCCGCGGCCA CATCCAGATG CCCGGTGCCA GCGCCCTGGT GCCCATCGAG 
GACTTGCTAC AGGCGCAATT TGAGGCGCAT CAGTTTATGA ATTTGTTTGA GGGGCAGCTG 
AGGCTGAACG TGAATTTGTT GTTGCATTTG TTGTACAAGA AGTTCTATCG GACTCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011393085.1
CDS	658..6057
Translation	MSSYKPHPLRQTQTADYSVNRQSSTTSTSSSGYSASSIGQSDISRPISSLSTATSVYSQYGHKRGQSEASRLPPSLSGGSHTLPSTPTSDNIYKNARQSLRPTSQASTGAAPASSSISTAGSRIAPRTPTPGHHDRGKSVDISKLSIGEYGTSTTHRERGQSLDNGKPLATERPRISPTGTVHERLASFDVSKSSVPERPRISPTGALHERGHTIDMGKLSISDNTRTSSGMSPTVTSPPATSPTTPSTRRPASMFLSRSDSVRGGSDNGSMALVHSSPIALARPDLEKLGRSSTSQLRTLSQFAQDGTAGELAIISPSQEVVGLRGRRRLQRADKSVAARQKNGGFGWETKWMDKQRQFLQAYEYLCHIGEAKEWIEDIIQRPIPPIIELEEALRDGVTLAEVVESLNPEKKFRIFRHPKLQYRHSDNIAIFFRYLDEVELPDLFRFELIDLYEKKNIPKVIYCLHALSWLLFRKGIVDFRIGNLVGQLEFEDHELEAMQKGLDKLGASMPSFGNMGADFGIEPAEPEETEEERIERELAENEEAIVELQAQARAALQRIQLGYTMQHLWDNEDWIIDLQSRIRGDWARQVADYRLQMKNFAVQLQSAARGFLARYRHAETKAQTSFAIELQKTARGFIARQQYAQTCFTIQLQKAARGYLARRQQTQTEEQFKGNEDVVLKLQSMVRAAKVRNVIREQHSQLETAAGPLRDIQATFRGFLARKQLAVQHEKTESEGATIAQLQSAVRGMILRKNLEEERASLESNIEAITSLQAAVRAMKARTEVDAQLQALEGFAPQWTAIQAAFRGKVARSDVDATKEELQKHSTEMGLLQSHIRAAAVRRDISEQLESIGETEDAIIDLQSMVRGMLERMRLSDDLDALTQETPNIIDLQAHIRGCLYRKECGELLDELAVNTEEITELQALARAMLLRSDVAMVLDELEQNEESVAAFQAAAKAFVVRAKYEEKKRFFRENMQKVVKIQSFVRAKLQGEAYKSLTSGKNPPVNAVKNFVHLLNDSDFDFNEEVEFERLRKTVVQQVRQNEMLEQYIDQLDIKIALLVKNKITLDEVVRHQHNYGGHANSLLANSVISNANQFDLKALNKSSRKKLESYQQLFFTLQTQPQYLAKLFRRIREQGITEKELKRIEHLMMGLFGYAQKRREEYYLLKLISRSIREEVEACAALQDYLRGNYFWTKLLQNYTRSPRDRKYLRDLLGPLIRDNIIEDPALDLESDPMQIYRSAINNEELRTGRPSQRPLDVPREVAIKDPETRELFIDHLRDLREICDQFLLALEDLLPRMPYGLRFVCRQTYEALCQHFQRDHPQNVLQFVSNWLWRFYLQPALLGPESYGVTEKQMSPLQKRNLGEVAKVLGQIASGRPFGGENIYLQPLNAFVAEAIERLAHITNSLIDVPDAEKTFDIDEFNDLYAKNKPTLYIKMADIFSIHNLIAAELPYLCPSRDDMLREIIQDLGNAKNNENEMTAGGAADIQLFLTPKLHDMEDPEAETKGLFMETKRCILYIIRVQTGNNLLEILVKPITPEDEQKWRTVLREDFGDDSNTRGAYSEANMIDVTRMSYYDLKRTALENILRLEQMGRISKQNYYQDILNAIALDIRTKSRRRVQRQRELEGVRQTLGNLHEKAKYLEQQRKSYDDYIEQAMATLQNKKGKKRFLLPFTKQYNHQRELERSGRVPKFGSFKYSARALSDKGVLVSWTGIPERDWGQINITISCDEVGIFSLEGSRGHIQMPGASALVPIEDLLQAQFEAHQFMNLFEGQLRLNVNLLLHLLYKKFYRTQ

Target ORF information:

RefSeq Version	XM_011394783.1
Organism	Neurospora crassa OR74A
Definition	Neurospora crassa OR74A Ras GTPase activating protein (NCU03116), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011394783.1

ORF Insert Sequence:

ATGTCGTCGT ACAAACCACA CCCCCTTCGT CAGACACAGA CCGCCGACTA CTCGGTTAAT 
AGACAATCCT CCACCACATC AACATCATCA TCAGGGTACT CGGCCTCTTC CATAGGACAG 
TCGGATATCA GTCGACCCAT TAGCTCCTTG TCTACAGCAA CCAGCGTCTA CTCCCAATAT 
GGACACAAGC GGGGCCAAAG CGAGGCGAGC CGACTACCCC CAAGCCTGTC TGGTGGCTCA 
CACACCCTCC CGTCCACGCC GACAAGTGAC AATATCTACA AGAATGCCCG CCAGTCCCTC 
CGACCGACAT CTCAAGCCTC TACGGGAGCA GCGCCCGCCA GCAGCAGCAT ATCGACTGCG 
GGTTCGAGAA TCGCGCCGCG CACGCCCACA CCAGGCCACC ACGATCGTGG AAAAAGTGTT 
GACATCAGCA AGCTGTCCAT CGGAGAGTAT GGGACCTCGA CTACCCATCG CGAACGCGGT 
CAGAGTCTCG ACAACGGCAA GCCTCTGGCG ACTGAGCGCC CACGGATATC GCCTACGGGA 
ACTGTTCATG AACGTCTAGC AAGCTTCGAT GTCAGCAAGT CCTCGGTACC GGAACGTCCG 
CGGATATCGC CTACGGGTGC CCTCCACGAG CGCGGTCACA CTATCGATAT GGGCAAATTA 
TCGATTAGCG ATAATACACG TACTTCGTCA GGCATGTCTC CGACCGTGAC ATCGCCTCCG 
GCAACTTCTC CAACGACCCC TTCAACGCGA AGGCCGGCTT CCATGTTTCT ATCCAGATCA 
GATTCTGTGC GTGGCGGAAG TGACAATGGG TCAATGGCTC TGGTACACTC GAGTCCCATT 
GCCCTTGCTC GTCCGGATCT GGAAAAATTA GGTCGTTCAT CGACAAGCCA ACTCCGAACA 
CTTTCTCAGT TCGCACAAGA TGGAACGGCG GGAGAGCTTG CCATCATATC TCCGTCGCAG 
GAAGTTGTTG GTCTTCGCGG ACGCCGTAGG TTGCAAAGAG CTGACAAGTC TGTAGCGGCG 
CGTCAAAAGA ATGGCGGCTT TGGTTGGGAG ACGAAGTGGA TGGACAAGCA ACGGCAGTTT 
CTACAGGCTT ATGAATACCT TTGCCACATT GGTGAAGCGA AGGAATGGAT TGAGGATATC 
ATACAGCGTC CGATCCCCCC GATTATCGAA TTAGAGGAGG CTTTGCGAGA TGGAGTTACT 
TTGGCCGAAG TGGTCGAATC CCTTAACCCT GAAAAGAAGT TCCGCATTTT CCGCCATCCT 
AAGCTTCAAT ACCGCCATTC AGACAATATC GCCATCTTCT TCCGTTACCT TGATGAAGTC 
GAACTTCCCG ACCTCTTCCG ATTTGAGCTT ATCGATCTGT ACGAGAAAAA GAACATCCCG 
AAAGTTATTT ACTGTCTCCA TGCTTTGAGT TGGCTTCTCT TCCGGAAAGG AATTGTCGAT 
TTCCGCATCG GGAATCTGGT CGGCCAGCTT GAGTTTGAAG ACCATGAGCT GGAGGCGATG 
CAGAAAGGCC TCGATAAGCT GGGGGCTAGT ATGCCTAGCT TCGGCAATAT GGGTGCCGAC 
TTTGGAATTG AGCCAGCTGA ACCCGAGGAG ACGGAGGAGG AACGCATTGA GAGGGAACTG 
GCCGAAAACG AAGAGGCCAT TGTAGAGTTA CAAGCCCAGG CCAGAGCGGC GCTTCAAAGG 
ATACAGCTTG GCTACACTAT GCAACATCTG TGGGATAATG AAGACTGGAT CATTGATCTC 
CAGTCTCGTA TTCGTGGTGA CTGGGCTCGC CAGGTGGCGG ATTACAGGCT CCAGATGAAG 
AATTTCGCGG TCCAGCTTCA AAGCGCAGCG CGCGGTTTCC TCGCCCGCTA TCGACATGCA 
GAAACAAAGG CACAAACAAG TTTTGCGATC GAGTTGCAGA AGACAGCTCG TGGATTTATC 
GCTCGTCAGC AATATGCACA AACATGTTTC ACGATTCAGC TACAAAAAGC GGCGCGTGGA 
TATCTTGCTC GTCGCCAGCA GACACAAACC GAGGAGCAGT TCAAGGGCAA CGAGGATGTG 
GTCTTGAAAC TCCAAAGTAT GGTCAGAGCG GCCAAGGTCC GCAATGTGAT TCGGGAGCAG 
CATTCACAGT TGGAGACGGC AGCGGGACCC CTGCGAGACA TTCAAGCAAC ATTTCGAGGA 
TTTCTGGCTC GAAAGCAGCT CGCTGTGCAA CATGAAAAGA CAGAGAGCGA AGGCGCTACG 
ATTGCTCAAC TTCAGAGCGC AGTTCGTGGT ATGATATTGA GGAAGAATCT GGAGGAAGAA 
AGAGCATCCT TGGAGAGCAA CATCGAAGCC ATCACTAGTC TTCAGGCTGC TGTAAGGGCC 
ATGAAAGCAA GAACTGAGGT CGATGCCCAG CTACAAGCGC TTGAAGGCTT TGCGCCACAA 
TGGACAGCTA TTCAAGCTGC CTTCCGCGGC AAGGTTGCTA GGTCCGACGT CGACGCTACT 
AAAGAAGAGT TGCAGAAGCA TTCAACTGAG ATGGGCCTTC TTCAGTCCCA CATTAGGGCA 
GCGGCAGTGC GGAGGGACAT ATCAGAGCAG CTTGAAAGCA TCGGGGAAAC GGAGGATGCC 
ATCATTGATC TTCAGTCTAT GGTCCGAGGT ATGCTTGAAC GTATGAGGTT ATCGGACGAT 
CTGGATGCTC TTACTCAGGA GACACCAAAC ATCATTGACC TTCAAGCACA CATCCGCGGA 
TGTCTTTATC GCAAGGAGTG TGGCGAGTTG TTGGATGAGC TTGCAGTCAA TACCGAGGAG 
ATCACCGAAC TGCAAGCCCT TGCTCGGGCC ATGTTGCTTC GGAGCGATGT TGCAATGGTC 
TTGGATGAAC TGGAACAAAA CGAGGAGTCT GTAGCGGCGT TCCAGGCTGC TGCCAAGGCT 
TTCGTGGTCA GAGCCAAGTA CGAGGAGAAG AAGCGCTTCT TCCGGGAGAA CATGCAAAAG 
GTTGTCAAGA TTCAGAGTTT CGTACGAGCC AAGCTTCAGG GTGAAGCCTA CAAGAGCTTG 
ACATCTGGAA AGAACCCACC AGTCAACGCC GTCAAGAACT TTGTCCATCT TCTGAACGAC 
AGCGATTTTG ACTTCAATGA GGAGGTAGAG TTTGAGCGCC TACGAAAGAC TGTTGTCCAA 
CAAGTTCGGC AAAACGAGAT GCTGGAACAA TACATCGACC AGCTAGACAT CAAGATCGCA 
CTTCTCGTCA AGAATAAAAT TACCCTTGAT GAAGTTGTCA GACATCAGCA CAACTATGGC 
GGACACGCAA ACAGCCTTCT CGCCAACAGT GTTATTTCAA ATGCTAATCA GTTTGACCTC 
AAGGCTTTGA ATAAGAGTTC CCGCAAGAAG CTTGAGTCCT ACCAGCAGCT GTTCTTCACC 
CTTCAGACGC AACCTCAGTA CCTTGCTAAG CTCTTCAGGC GTATCCGCGA GCAAGGAATA 
ACGGAAAAGG AGTTAAAGCG GATTGAACAC TTGATGATGG GGCTGTTCGG CTATGCGCAA 
AAGCGGAGAG AGGAATACTA CTTATTGAAG CTTATTTCCA GGTCGATAAG GGAAGAAGTC 
GAAGCATGTG CCGCTCTGCA GGATTATCTG AGGGGCAACT ACTTCTGGAC CAAGCTGCTC 
CAGAATTACA CCCGATCCCC TCGTGATAGG AAATATCTCA GAGATCTCTT GGGACCCCTC 
ATCAGAGACA ACATTATCGA GGATCCAGCG CTGGATCTTG AAAGCGACCC CATGCAGATC 
TACCGGTCTG CTATCAACAA TGAGGAGTTG AGGACCGGTC GGCCCAGCCA AAGACCTCTG 
GATGTCCCTC GTGAGGTTGC CATCAAGGAT CCTGAAACCA GAGAGCTCTT CATTGATCAT 
CTGCGGGATC TCCGAGAGAT CTGCGATCAG TTTCTCCTTG CCCTGGAGGA CCTGCTTCCC 
CGCATGCCCT ACGGCCTGAG ATTTGTGTGC CGGCAAACGT ACGAAGCTCT CTGCCAGCAT 
TTCCAGAGGG ACCACCCGCA AAATGTGCTC CAGTTTGTAA GCAACTGGCT CTGGCGCTTC 
TACCTCCAAC CTGCTCTCCT TGGCCCAGAG TCATATGGAG TCACGGAAAA GCAAATGAGT 
CCCCTCCAGA AGCGTAACCT CGGCGAGGTT GCAAAAGTCC TGGGCCAGAT TGCATCAGGC 
AGACCATTTG GCGGAGAGAA CATCTATCTC CAGCCTCTCA ATGCTTTCGT CGCCGAAGCA 
ATCGAGCGCT TGGCTCACAT CACCAATTCC CTTATCGATG TACCTGACGC TGAGAAGACA 
TTCGACATTG ACGAGTTCAA CGATCTCTAC GCCAAGAACA AGCCAACACT GTACATCAAG 
ATGGCGGATA TCTTTTCCAT CCACAATCTC ATCGCTGCCG AGTTGCCTTA TCTGTGCCCG 
AGCAGAGATG ATATGCTGCG CGAGATCATT CAAGATCTCG GTAACGCCAA GAATAATGAG 
AATGAGATGA CCGCAGGCGG GGCCGCTGAT ATTCAGCTGT TCCTGACTCC CAAACTGCAC 
GATATGGAGG ATCCAGAAGC TGAGACTAAG GGGCTGTTTA TGGAGACCAA GAGGTGCATT 
CTGTACATCA TCCGGGTGCA AACGGGTAAC AACCTGCTTG AGATTCTTGT CAAGCCAATT 
ACCCCGGAGG ATGAGCAGAA GTGGAGGACA GTTCTGCGCG AAGACTTTGG TGACGACTCG 
AACACCCGAG GCGCTTATTC GGAGGCGAAC ATGATTGATG TTACGCGCAT GAGTTACTAC 
GACCTAAAGA GGACAGCACT CGAGAACATC CTCAGGTTGG AGCAGATGGG TCGTATCTCC 
AAGCAAAACT ACTACCAGGA TATCCTCAAC GCCATTGCGC TGGACATTCG CACAAAGAGC 
AGGAGAAGGG TTCAGAGACA GAGAGAGCTC GAGGGTGTCC GTCAGACACT CGGCAATCTT 
CACGAGAAGG CAAAGTATCT GGAGCAACAG CGCAAGAGCT ACGATGATTA CATCGAGCAA 
GCCATGGCTA CGCTGCAGAA CAAGAAGGGC AAGAAACGTT TCCTTCTCCC CTTCACCAAG 
CAATACAACC ACCAGCGCGA GCTCGAGCGC TCCGGCCGCG TCCCCAAGTT CGGCTCCTTC 
AAGTACTCGG CCCGCGCCCT CTCGGACAAG GGCGTGCTGG TCTCGTGGAC GGGCATCCCC 
GAGCGCGACT GGGGTCAAAT CAACATCACC ATCTCGTGCG ACGAGGTCGG CATTTTTAGT 
CTTGAGGGCA GCCGCGGCCA CATCCAGATG CCCGGTGCCA GCGCCCTGGT GCCCATCGAG 
GACTTGCTAC AGGCGCAATT TGAGGCGCAT CAGTTTATGA ATTTGTTTGA GGGGCAGCTG 
AGGCTGAACG TGAATTTGTT GTTGCATTTG TTGTACAAGA AGTTCTATCG GACTCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NCU03116 ( XM_011394784.1 ) cDNA ORF clone, Neurospora crassa OR74A -> XP_011393086.1 Neurospora crassa OR74A Ras GTPase activating protein, variant 2 (NCU03116), mRNA.

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CloneID	ONf13025
Clone ID Related Accession (Same CDS sequence)	XM_011394782.1 , XM_011394785.1 , XM_011394783.1 , XM_011394784.1
Accession Version	XM_011394784.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5400bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-10
Organism	Neurospora crassa OR74A
Product	Ras GTPase activating protein, variant 2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026501).

ATGTCGTCGT ACAAACCACA CCCCCTTCGT CAGACACAGA CCGCCGACTA CTCGGTTAAT 
AGACAATCCT CCACCACATC AACATCATCA TCAGGGTACT CGGCCTCTTC CATAGGACAG 
TCGGATATCA GTCGACCCAT TAGCTCCTTG TCTACAGCAA CCAGCGTCTA CTCCCAATAT 
GGACACAAGC GGGGCCAAAG CGAGGCGAGC CGACTACCCC CAAGCCTGTC TGGTGGCTCA 
CACACCCTCC CGTCCACGCC GACAAGTGAC AATATCTACA AGAATGCCCG CCAGTCCCTC 
CGACCGACAT CTCAAGCCTC TACGGGAGCA GCGCCCGCCA GCAGCAGCAT ATCGACTGCG 
GGTTCGAGAA TCGCGCCGCG CACGCCCACA CCAGGCCACC ACGATCGTGG AAAAAGTGTT 
GACATCAGCA AGCTGTCCAT CGGAGAGTAT GGGACCTCGA CTACCCATCG CGAACGCGGT 
CAGAGTCTCG ACAACGGCAA GCCTCTGGCG ACTGAGCGCC CACGGATATC GCCTACGGGA 
ACTGTTCATG AACGTCTAGC AAGCTTCGAT GTCAGCAAGT CCTCGGTACC GGAACGTCCG 
CGGATATCGC CTACGGGTGC CCTCCACGAG CGCGGTCACA CTATCGATAT GGGCAAATTA 
TCGATTAGCG ATAATACACG TACTTCGTCA GGCATGTCTC CGACCGTGAC ATCGCCTCCG 
GCAACTTCTC CAACGACCCC TTCAACGCGA AGGCCGGCTT CCATGTTTCT ATCCAGATCA 
GATTCTGTGC GTGGCGGAAG TGACAATGGG TCAATGGCTC TGGTACACTC GAGTCCCATT 
GCCCTTGCTC GTCCGGATCT GGAAAAATTA GGTCGTTCAT CGACAAGCCA ACTCCGAACA 
CTTTCTCAGT TCGCACAAGA TGGAACGGCG GGAGAGCTTG CCATCATATC TCCGTCGCAG 
GAAGTTGTTG GTCTTCGCGG ACGCCGTAGG TTGCAAAGAG CTGACAAGTC TGTAGCGGCG 
CGTCAAAAGA ATGGCGGCTT TGGTTGGGAG ACGAAGTGGA TGGACAAGCA ACGGCAGTTT 
CTACAGGCTT ATGAATACCT TTGCCACATT GGTGAAGCGA AGGAATGGAT TGAGGATATC 
ATACAGCGTC CGATCCCCCC GATTATCGAA TTAGAGGAGG CTTTGCGAGA TGGAGTTACT 
TTGGCCGAAG TGGTCGAATC CCTTAACCCT GAAAAGAAGT TCCGCATTTT CCGCCATCCT 
AAGCTTCAAT ACCGCCATTC AGACAATATC GCCATCTTCT TCCGTTACCT TGATGAAGTC 
GAACTTCCCG ACCTCTTCCG ATTTGAGCTT ATCGATCTGT ACGAGAAAAA GAACATCCCG 
AAAGTTATTT ACTGTCTCCA TGCTTTGAGT TGGCTTCTCT TCCGGAAAGG AATTGTCGAT 
TTCCGCATCG GGAATCTGGT CGGCCAGCTT GAGTTTGAAG ACCATGAGCT GGAGGCGATG 
CAGAAAGGCC TCGATAAGCT GGGGGCTAGT ATGCCTAGCT TCGGCAATAT GGGTGCCGAC 
TTTGGAATTG AGCCAGCTGA ACCCGAGGAG ACGGAGGAGG AACGCATTGA GAGGGAACTG 
GCCGAAAACG AAGAGGCCAT TGTAGAGTTA CAAGCCCAGG CCAGAGCGGC GCTTCAAAGG 
ATACAGCTTG GCTACACTAT GCAACATCTG TGGGATAATG AAGACTGGAT CATTGATCTC 
CAGTCTCGTA TTCGTGGTGA CTGGGCTCGC CAGGTGGCGG ATTACAGGCT CCAGATGAAG 
AATTTCGCGG TCCAGCTTCA AAGCGCAGCG CGCGGTTTCC TCGCCCGCTA TCGACATGCA 
GAAACAAAGG CACAAACAAG TTTTGCGATC GAGTTGCAGA AGACAGCTCG TGGATTTATC 
GCTCGTCAGC AATATGCACA AACATGTTTC ACGATTCAGC TACAAAAAGC GGCGCGTGGA 
TATCTTGCTC GTCGCCAGCA GACACAAACC GAGGAGCAGT TCAAGGGCAA CGAGGATGTG 
GTCTTGAAAC TCCAAAGTAT GGTCAGAGCG GCCAAGGTCC GCAATGTGAT TCGGGAGCAG 
CATTCACAGT TGGAGACGGC AGCGGGACCC CTGCGAGACA TTCAAGCAAC ATTTCGAGGA 
TTTCTGGCTC GAAAGCAGCT CGCTGTGCAA CATGAAAAGA CAGAGAGCGA AGGCGCTACG 
ATTGCTCAAC TTCAGAGCGC AGTTCGTGGT ATGATATTGA GGAAGAATCT GGAGGAAGAA 
AGAGCATCCT TGGAGAGCAA CATCGAAGCC ATCACTAGTC TTCAGGCTGC TGTAAGGGCC 
ATGAAAGCAA GAACTGAGGT CGATGCCCAG CTACAAGCGC TTGAAGGCTT TGCGCCACAA 
TGGACAGCTA TTCAAGCTGC CTTCCGCGGC AAGGTTGCTA GGTCCGACGT CGACGCTACT 
AAAGAAGAGT TGCAGAAGCA TTCAACTGAG ATGGGCCTTC TTCAGTCCCA CATTAGGGCA 
GCGGCAGTGC GGAGGGACAT ATCAGAGCAG CTTGAAAGCA TCGGGGAAAC GGAGGATGCC 
ATCATTGATC TTCAGTCTAT GGTCCGAGGT ATGCTTGAAC GTATGAGGTT ATCGGACGAT 
CTGGATGCTC TTACTCAGGA GACACCAAAC ATCATTGACC TTCAAGCACA CATCCGCGGA 
TGTCTTTATC GCAAGGAGTG TGGCGAGTTG TTGGATGAGC TTGCAGTCAA TACCGAGGAG 
ATCACCGAAC TGCAAGCCCT TGCTCGGGCC ATGTTGCTTC GGAGCGATGT TGCAATGGTC 
TTGGATGAAC TGGAACAAAA CGAGGAGTCT GTAGCGGCGT TCCAGGCTGC TGCCAAGGCT 
TTCGTGGTCA GAGCCAAGTA CGAGGAGAAG AAGCGCTTCT TCCGGGAGAA CATGCAAAAG 
GTTGTCAAGA TTCAGAGTTT CGTACGAGCC AAGCTTCAGG GTGAAGCCTA CAAGAGCTTG 
ACATCTGGAA AGAACCCACC AGTCAACGCC GTCAAGAACT TTGTCCATCT TCTGAACGAC 
AGCGATTTTG ACTTCAATGA GGAGGTAGAG TTTGAGCGCC TACGAAAGAC TGTTGTCCAA 
CAAGTTCGGC AAAACGAGAT GCTGGAACAA TACATCGACC AGCTAGACAT CAAGATCGCA 
CTTCTCGTCA AGAATAAAAT TACCCTTGAT GAAGTTGTCA GACATCAGCA CAACTATGGC 
GGACACGCAA ACAGCCTTCT CGCCAACAGT GTTATTTCAA ATGCTAATCA GTTTGACCTC 
AAGGCTTTGA ATAAGAGTTC CCGCAAGAAG CTTGAGTCCT ACCAGCAGCT GTTCTTCACC 
CTTCAGACGC AACCTCAGTA CCTTGCTAAG CTCTTCAGGC GTATCCGCGA GCAAGGAATA 
ACGGAAAAGG AGTTAAAGCG GATTGAACAC TTGATGATGG GGCTGTTCGG CTATGCGCAA 
AAGCGGAGAG AGGAATACTA CTTATTGAAG CTTATTTCCA GGTCGATAAG GGAAGAAGTC 
GAAGCATGTG CCGCTCTGCA GGATTATCTG AGGGGCAACT ACTTCTGGAC CAAGCTGCTC 
CAGAATTACA CCCGATCCCC TCGTGATAGG AAATATCTCA GAGATCTCTT GGGACCCCTC 
ATCAGAGACA ACATTATCGA GGATCCAGCG CTGGATCTTG AAAGCGACCC CATGCAGATC 
TACCGGTCTG CTATCAACAA TGAGGAGTTG AGGACCGGTC GGCCCAGCCA AAGACCTCTG 
GATGTCCCTC GTGAGGTTGC CATCAAGGAT CCTGAAACCA GAGAGCTCTT CATTGATCAT 
CTGCGGGATC TCCGAGAGAT CTGCGATCAG TTTCTCCTTG CCCTGGAGGA CCTGCTTCCC 
CGCATGCCCT ACGGCCTGAG ATTTGTGTGC CGGCAAACGT ACGAAGCTCT CTGCCAGCAT 
TTCCAGAGGG ACCACCCGCA AAATGTGCTC CAGTTTGTAA GCAACTGGCT CTGGCGCTTC 
TACCTCCAAC CTGCTCTCCT TGGCCCAGAG TCATATGGAG TCACGGAAAA GCAAATGAGT 
CCCCTCCAGA AGCGTAACCT CGGCGAGGTT GCAAAAGTCC TGGGCCAGAT TGCATCAGGC 
AGACCATTTG GCGGAGAGAA CATCTATCTC CAGCCTCTCA ATGCTTTCGT CGCCGAAGCA 
ATCGAGCGCT TGGCTCACAT CACCAATTCC CTTATCGATG TACCTGACGC TGAGAAGACA 
TTCGACATTG ACGAGTTCAA CGATCTCTAC GCCAAGAACA AGCCAACACT GTACATCAAG 
ATGGCGGATA TCTTTTCCAT CCACAATCTC ATCGCTGCCG AGTTGCCTTA TCTGTGCCCG 
AGCAGAGATG ATATGCTGCG CGAGATCATT CAAGATCTCG GTAACGCCAA GAATAATGAG 
AATGAGATGA CCGCAGGCGG GGCCGCTGAT ATTCAGCTGT TCCTGACTCC CAAACTGCAC 
GATATGGAGG ATCCAGAAGC TGAGACTAAG GGGCTGTTTA TGGAGACCAA GAGGTGCATT 
CTGTACATCA TCCGGGTGCA AACGGGTAAC AACCTGCTTG AGATTCTTGT CAAGCCAATT 
ACCCCGGAGG ATGAGCAGAA GTGGAGGACA GTTCTGCGCG AAGACTTTGG TGACGACTCG 
AACACCCGAG GCGCTTATTC GGAGGCGAAC ATGATTGATG TTACGCGCAT GAGTTACTAC 
GACCTAAAGA GGACAGCACT CGAGAACATC CTCAGGTTGG AGCAGATGGG TCGTATCTCC 
AAGCAAAACT ACTACCAGGA TATCCTCAAC GCCATTGCGC TGGACATTCG CACAAAGAGC 
AGGAGAAGGG TTCAGAGACA GAGAGAGCTC GAGGGTGTCC GTCAGACACT CGGCAATCTT 
CACGAGAAGG CAAAGTATCT GGAGCAACAG CGCAAGAGCT ACGATGATTA CATCGAGCAA 
GCCATGGCTA CGCTGCAGAA CAAGAAGGGC AAGAAACGTT TCCTTCTCCC CTTCACCAAG 
CAATACAACC ACCAGCGCGA GCTCGAGCGC TCCGGCCGCG TCCCCAAGTT CGGCTCCTTC 
AAGTACTCGG CCCGCGCCCT CTCGGACAAG GGCGTGCTGG TCTCGTGGAC GGGCATCCCC 
GAGCGCGACT GGGGTCAAAT CAACATCACC ATCTCGTGCG ACGAGGTCGG CATTTTTAGT 
CTTGAGGGCA GCCGCGGCCA CATCCAGATG CCCGGTGCCA GCGCCCTGGT GCCCATCGAG 
GACTTGCTAC AGGCGCAATT TGAGGCGCAT CAGTTTATGA ATTTGTTTGA GGGGCAGCTG 
AGGCTGAACG TGAATTTGTT GTTGCATTTG TTGTACAAGA AGTTCTATCG GACTCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011393086.1
CDS	248..5647
Translation	MSSYKPHPLRQTQTADYSVNRQSSTTSTSSSGYSASSIGQSDISRPISSLSTATSVYSQYGHKRGQSEASRLPPSLSGGSHTLPSTPTSDNIYKNARQSLRPTSQASTGAAPASSSISTAGSRIAPRTPTPGHHDRGKSVDISKLSIGEYGTSTTHRERGQSLDNGKPLATERPRISPTGTVHERLASFDVSKSSVPERPRISPTGALHERGHTIDMGKLSISDNTRTSSGMSPTVTSPPATSPTTPSTRRPASMFLSRSDSVRGGSDNGSMALVHSSPIALARPDLEKLGRSSTSQLRTLSQFAQDGTAGELAIISPSQEVVGLRGRRRLQRADKSVAARQKNGGFGWETKWMDKQRQFLQAYEYLCHIGEAKEWIEDIIQRPIPPIIELEEALRDGVTLAEVVESLNPEKKFRIFRHPKLQYRHSDNIAIFFRYLDEVELPDLFRFELIDLYEKKNIPKVIYCLHALSWLLFRKGIVDFRIGNLVGQLEFEDHELEAMQKGLDKLGASMPSFGNMGADFGIEPAEPEETEEERIERELAENEEAIVELQAQARAALQRIQLGYTMQHLWDNEDWIIDLQSRIRGDWARQVADYRLQMKNFAVQLQSAARGFLARYRHAETKAQTSFAIELQKTARGFIARQQYAQTCFTIQLQKAARGYLARRQQTQTEEQFKGNEDVVLKLQSMVRAAKVRNVIREQHSQLETAAGPLRDIQATFRGFLARKQLAVQHEKTESEGATIAQLQSAVRGMILRKNLEEERASLESNIEAITSLQAAVRAMKARTEVDAQLQALEGFAPQWTAIQAAFRGKVARSDVDATKEELQKHSTEMGLLQSHIRAAAVRRDISEQLESIGETEDAIIDLQSMVRGMLERMRLSDDLDALTQETPNIIDLQAHIRGCLYRKECGELLDELAVNTEEITELQALARAMLLRSDVAMVLDELEQNEESVAAFQAAAKAFVVRAKYEEKKRFFRENMQKVVKIQSFVRAKLQGEAYKSLTSGKNPPVNAVKNFVHLLNDSDFDFNEEVEFERLRKTVVQQVRQNEMLEQYIDQLDIKIALLVKNKITLDEVVRHQHNYGGHANSLLANSVISNANQFDLKALNKSSRKKLESYQQLFFTLQTQPQYLAKLFRRIREQGITEKELKRIEHLMMGLFGYAQKRREEYYLLKLISRSIREEVEACAALQDYLRGNYFWTKLLQNYTRSPRDRKYLRDLLGPLIRDNIIEDPALDLESDPMQIYRSAINNEELRTGRPSQRPLDVPREVAIKDPETRELFIDHLRDLREICDQFLLALEDLLPRMPYGLRFVCRQTYEALCQHFQRDHPQNVLQFVSNWLWRFYLQPALLGPESYGVTEKQMSPLQKRNLGEVAKVLGQIASGRPFGGENIYLQPLNAFVAEAIERLAHITNSLIDVPDAEKTFDIDEFNDLYAKNKPTLYIKMADIFSIHNLIAAELPYLCPSRDDMLREIIQDLGNAKNNENEMTAGGAADIQLFLTPKLHDMEDPEAETKGLFMETKRCILYIIRVQTGNNLLEILVKPITPEDEQKWRTVLREDFGDDSNTRGAYSEANMIDVTRMSYYDLKRTALENILRLEQMGRISKQNYYQDILNAIALDIRTKSRRRVQRQRELEGVRQTLGNLHEKAKYLEQQRKSYDDYIEQAMATLQNKKGKKRFLLPFTKQYNHQRELERSGRVPKFGSFKYSARALSDKGVLVSWTGIPERDWGQINITISCDEVGIFSLEGSRGHIQMPGASALVPIEDLLQAQFEAHQFMNLFEGQLRLNVNLLLHLLYKKFYRTQ

Target ORF information:

RefSeq Version	XM_011394784.1
Organism	Neurospora crassa OR74A
Definition	Neurospora crassa OR74A Ras GTPase activating protein, variant 2 (NCU03116), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011394784.1

ORF Insert Sequence:

ATGTCGTCGT ACAAACCACA CCCCCTTCGT CAGACACAGA CCGCCGACTA CTCGGTTAAT 
AGACAATCCT CCACCACATC AACATCATCA TCAGGGTACT CGGCCTCTTC CATAGGACAG 
TCGGATATCA GTCGACCCAT TAGCTCCTTG TCTACAGCAA CCAGCGTCTA CTCCCAATAT 
GGACACAAGC GGGGCCAAAG CGAGGCGAGC CGACTACCCC CAAGCCTGTC TGGTGGCTCA 
CACACCCTCC CGTCCACGCC GACAAGTGAC AATATCTACA AGAATGCCCG CCAGTCCCTC 
CGACCGACAT CTCAAGCCTC TACGGGAGCA GCGCCCGCCA GCAGCAGCAT ATCGACTGCG 
GGTTCGAGAA TCGCGCCGCG CACGCCCACA CCAGGCCACC ACGATCGTGG AAAAAGTGTT 
GACATCAGCA AGCTGTCCAT CGGAGAGTAT GGGACCTCGA CTACCCATCG CGAACGCGGT 
CAGAGTCTCG ACAACGGCAA GCCTCTGGCG ACTGAGCGCC CACGGATATC GCCTACGGGA 
ACTGTTCATG AACGTCTAGC AAGCTTCGAT GTCAGCAAGT CCTCGGTACC GGAACGTCCG 
CGGATATCGC CTACGGGTGC CCTCCACGAG CGCGGTCACA CTATCGATAT GGGCAAATTA 
TCGATTAGCG ATAATACACG TACTTCGTCA GGCATGTCTC CGACCGTGAC ATCGCCTCCG 
GCAACTTCTC CAACGACCCC TTCAACGCGA AGGCCGGCTT CCATGTTTCT ATCCAGATCA 
GATTCTGTGC GTGGCGGAAG TGACAATGGG TCAATGGCTC TGGTACACTC GAGTCCCATT 
GCCCTTGCTC GTCCGGATCT GGAAAAATTA GGTCGTTCAT CGACAAGCCA ACTCCGAACA 
CTTTCTCAGT TCGCACAAGA TGGAACGGCG GGAGAGCTTG CCATCATATC TCCGTCGCAG 
GAAGTTGTTG GTCTTCGCGG ACGCCGTAGG TTGCAAAGAG CTGACAAGTC TGTAGCGGCG 
CGTCAAAAGA ATGGCGGCTT TGGTTGGGAG ACGAAGTGGA TGGACAAGCA ACGGCAGTTT 
CTACAGGCTT ATGAATACCT TTGCCACATT GGTGAAGCGA AGGAATGGAT TGAGGATATC 
ATACAGCGTC CGATCCCCCC GATTATCGAA TTAGAGGAGG CTTTGCGAGA TGGAGTTACT 
TTGGCCGAAG TGGTCGAATC CCTTAACCCT GAAAAGAAGT TCCGCATTTT CCGCCATCCT 
AAGCTTCAAT ACCGCCATTC AGACAATATC GCCATCTTCT TCCGTTACCT TGATGAAGTC 
GAACTTCCCG ACCTCTTCCG ATTTGAGCTT ATCGATCTGT ACGAGAAAAA GAACATCCCG 
AAAGTTATTT ACTGTCTCCA TGCTTTGAGT TGGCTTCTCT TCCGGAAAGG AATTGTCGAT 
TTCCGCATCG GGAATCTGGT CGGCCAGCTT GAGTTTGAAG ACCATGAGCT GGAGGCGATG 
CAGAAAGGCC TCGATAAGCT GGGGGCTAGT ATGCCTAGCT TCGGCAATAT GGGTGCCGAC 
TTTGGAATTG AGCCAGCTGA ACCCGAGGAG ACGGAGGAGG AACGCATTGA GAGGGAACTG 
GCCGAAAACG AAGAGGCCAT TGTAGAGTTA CAAGCCCAGG CCAGAGCGGC GCTTCAAAGG 
ATACAGCTTG GCTACACTAT GCAACATCTG TGGGATAATG AAGACTGGAT CATTGATCTC 
CAGTCTCGTA TTCGTGGTGA CTGGGCTCGC CAGGTGGCGG ATTACAGGCT CCAGATGAAG 
AATTTCGCGG TCCAGCTTCA AAGCGCAGCG CGCGGTTTCC TCGCCCGCTA TCGACATGCA 
GAAACAAAGG CACAAACAAG TTTTGCGATC GAGTTGCAGA AGACAGCTCG TGGATTTATC 
GCTCGTCAGC AATATGCACA AACATGTTTC ACGATTCAGC TACAAAAAGC GGCGCGTGGA 
TATCTTGCTC GTCGCCAGCA GACACAAACC GAGGAGCAGT TCAAGGGCAA CGAGGATGTG 
GTCTTGAAAC TCCAAAGTAT GGTCAGAGCG GCCAAGGTCC GCAATGTGAT TCGGGAGCAG 
CATTCACAGT TGGAGACGGC AGCGGGACCC CTGCGAGACA TTCAAGCAAC ATTTCGAGGA 
TTTCTGGCTC GAAAGCAGCT CGCTGTGCAA CATGAAAAGA CAGAGAGCGA AGGCGCTACG 
ATTGCTCAAC TTCAGAGCGC AGTTCGTGGT ATGATATTGA GGAAGAATCT GGAGGAAGAA 
AGAGCATCCT TGGAGAGCAA CATCGAAGCC ATCACTAGTC TTCAGGCTGC TGTAAGGGCC 
ATGAAAGCAA GAACTGAGGT CGATGCCCAG CTACAAGCGC TTGAAGGCTT TGCGCCACAA 
TGGACAGCTA TTCAAGCTGC CTTCCGCGGC AAGGTTGCTA GGTCCGACGT CGACGCTACT 
AAAGAAGAGT TGCAGAAGCA TTCAACTGAG ATGGGCCTTC TTCAGTCCCA CATTAGGGCA 
GCGGCAGTGC GGAGGGACAT ATCAGAGCAG CTTGAAAGCA TCGGGGAAAC GGAGGATGCC 
ATCATTGATC TTCAGTCTAT GGTCCGAGGT ATGCTTGAAC GTATGAGGTT ATCGGACGAT 
CTGGATGCTC TTACTCAGGA GACACCAAAC ATCATTGACC TTCAAGCACA CATCCGCGGA 
TGTCTTTATC GCAAGGAGTG TGGCGAGTTG TTGGATGAGC TTGCAGTCAA TACCGAGGAG 
ATCACCGAAC TGCAAGCCCT TGCTCGGGCC ATGTTGCTTC GGAGCGATGT TGCAATGGTC 
TTGGATGAAC TGGAACAAAA CGAGGAGTCT GTAGCGGCGT TCCAGGCTGC TGCCAAGGCT 
TTCGTGGTCA GAGCCAAGTA CGAGGAGAAG AAGCGCTTCT TCCGGGAGAA CATGCAAAAG 
GTTGTCAAGA TTCAGAGTTT CGTACGAGCC AAGCTTCAGG GTGAAGCCTA CAAGAGCTTG 
ACATCTGGAA AGAACCCACC AGTCAACGCC GTCAAGAACT TTGTCCATCT TCTGAACGAC 
AGCGATTTTG ACTTCAATGA GGAGGTAGAG TTTGAGCGCC TACGAAAGAC TGTTGTCCAA 
CAAGTTCGGC AAAACGAGAT GCTGGAACAA TACATCGACC AGCTAGACAT CAAGATCGCA 
CTTCTCGTCA AGAATAAAAT TACCCTTGAT GAAGTTGTCA GACATCAGCA CAACTATGGC 
GGACACGCAA ACAGCCTTCT CGCCAACAGT GTTATTTCAA ATGCTAATCA GTTTGACCTC 
AAGGCTTTGA ATAAGAGTTC CCGCAAGAAG CTTGAGTCCT ACCAGCAGCT GTTCTTCACC 
CTTCAGACGC AACCTCAGTA CCTTGCTAAG CTCTTCAGGC GTATCCGCGA GCAAGGAATA 
ACGGAAAAGG AGTTAAAGCG GATTGAACAC TTGATGATGG GGCTGTTCGG CTATGCGCAA 
AAGCGGAGAG AGGAATACTA CTTATTGAAG CTTATTTCCA GGTCGATAAG GGAAGAAGTC 
GAAGCATGTG CCGCTCTGCA GGATTATCTG AGGGGCAACT ACTTCTGGAC CAAGCTGCTC 
CAGAATTACA CCCGATCCCC TCGTGATAGG AAATATCTCA GAGATCTCTT GGGACCCCTC 
ATCAGAGACA ACATTATCGA GGATCCAGCG CTGGATCTTG AAAGCGACCC CATGCAGATC 
TACCGGTCTG CTATCAACAA TGAGGAGTTG AGGACCGGTC GGCCCAGCCA AAGACCTCTG 
GATGTCCCTC GTGAGGTTGC CATCAAGGAT CCTGAAACCA GAGAGCTCTT CATTGATCAT 
CTGCGGGATC TCCGAGAGAT CTGCGATCAG TTTCTCCTTG CCCTGGAGGA CCTGCTTCCC 
CGCATGCCCT ACGGCCTGAG ATTTGTGTGC CGGCAAACGT ACGAAGCTCT CTGCCAGCAT 
TTCCAGAGGG ACCACCCGCA AAATGTGCTC CAGTTTGTAA GCAACTGGCT CTGGCGCTTC 
TACCTCCAAC CTGCTCTCCT TGGCCCAGAG TCATATGGAG TCACGGAAAA GCAAATGAGT 
CCCCTCCAGA AGCGTAACCT CGGCGAGGTT GCAAAAGTCC TGGGCCAGAT TGCATCAGGC 
AGACCATTTG GCGGAGAGAA CATCTATCTC CAGCCTCTCA ATGCTTTCGT CGCCGAAGCA 
ATCGAGCGCT TGGCTCACAT CACCAATTCC CTTATCGATG TACCTGACGC TGAGAAGACA 
TTCGACATTG ACGAGTTCAA CGATCTCTAC GCCAAGAACA AGCCAACACT GTACATCAAG 
ATGGCGGATA TCTTTTCCAT CCACAATCTC ATCGCTGCCG AGTTGCCTTA TCTGTGCCCG 
AGCAGAGATG ATATGCTGCG CGAGATCATT CAAGATCTCG GTAACGCCAA GAATAATGAG 
AATGAGATGA CCGCAGGCGG GGCCGCTGAT ATTCAGCTGT TCCTGACTCC CAAACTGCAC 
GATATGGAGG ATCCAGAAGC TGAGACTAAG GGGCTGTTTA TGGAGACCAA GAGGTGCATT 
CTGTACATCA TCCGGGTGCA AACGGGTAAC AACCTGCTTG AGATTCTTGT CAAGCCAATT 
ACCCCGGAGG ATGAGCAGAA GTGGAGGACA GTTCTGCGCG AAGACTTTGG TGACGACTCG 
AACACCCGAG GCGCTTATTC GGAGGCGAAC ATGATTGATG TTACGCGCAT GAGTTACTAC 
GACCTAAAGA GGACAGCACT CGAGAACATC CTCAGGTTGG AGCAGATGGG TCGTATCTCC 
AAGCAAAACT ACTACCAGGA TATCCTCAAC GCCATTGCGC TGGACATTCG CACAAAGAGC 
AGGAGAAGGG TTCAGAGACA GAGAGAGCTC GAGGGTGTCC GTCAGACACT CGGCAATCTT 
CACGAGAAGG CAAAGTATCT GGAGCAACAG CGCAAGAGCT ACGATGATTA CATCGAGCAA 
GCCATGGCTA CGCTGCAGAA CAAGAAGGGC AAGAAACGTT TCCTTCTCCC CTTCACCAAG 
CAATACAACC ACCAGCGCGA GCTCGAGCGC TCCGGCCGCG TCCCCAAGTT CGGCTCCTTC 
AAGTACTCGG CCCGCGCCCT CTCGGACAAG GGCGTGCTGG TCTCGTGGAC GGGCATCCCC 
GAGCGCGACT GGGGTCAAAT CAACATCACC ATCTCGTGCG ACGAGGTCGG CATTTTTAGT 
CTTGAGGGCA GCCGCGGCCA CATCCAGATG CCCGGTGCCA GCGCCCTGGT GCCCATCGAG 
GACTTGCTAC AGGCGCAATT TGAGGCGCAT CAGTTTATGA ATTTGTTTGA GGGGCAGCTG 
AGGCTGAACG TGAATTTGTT GTTGCATTTG TTGTACAAGA AGTTCTATCG GACTCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Nature422(6934)859-68(2003 Apr)
Galagan JE,Calvo SE,Borkovich KA,Selker EU,Read ND,Jaffe D,FitzHugh W,Ma LJ,Smirnov S,Purcell S,Rehman B,Elkins T,Engels R,Wang S,Nielsen CB,Butler J,Endrizzi M,Qui D,Ianakiev P,Bell-Pedersen D,Nelson MA,Werner-Washburne M,Selitrennikoff CP,Kinsey JA,Braun EL,Zelter A,Schulte U,Kothe GO,Jedd G,Mewes W,Staben C,Marcotte E,Greenberg D,Roy A,Foley K,Naylor J,Stange-Thomann N,Barrett R,Gnerre S,Kamal M,Kamvysselis M,Mauceli E,Bielke C,Rudd S,Frishman D,Krystofova S,Rasmussen C,Metzenberg RL,Perkins DD,Kroken S,Cogoni C,Macino G,Catcheside D,Li W,Pratt RJ,Osmani SA,DeSouza CP,Glass L,Orbach MJ,Berglund JA,Voelker R,Yarden O,Plamann M,Seiler S,Dunlap J,Radford A,Aramayo R,Natvig DO,Alex LA,Mannhaupt G,Ebbole DJ,Freitag M,Paulsen I,Sachs MS,Lander ES,Nusbaum C,Birren B

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NCU03116 cDNA ORF clone, Neurospora crassa OR74A

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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