tef-1 cDNA ORF clone, Neurospora crassa OR74A

The following tef-1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the tef-1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ONf08160 XM_959775.3
Latest version!
Neurospora crassa OR74A elongation factor 1 alpha (tef-1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $258.30
$369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ONf08160
    Clone ID Related Accession (Same CDS sequence) XM_959775.3
    Accession Version XM_959775.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1383bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-10
    Organism Neurospora crassa OR74A
    Product elongation factor 1 alpha
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026501). On Feb 23, 2015 this sequence version replaced XM_959775.2.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGGGCAAGG AGGACAAGAC TCACATCAAC GTCGTCGTTA TCGGCCACGT CGATTCCGGC 
    AAGTCTACCA CTACCGGTCA CTTGATCTAC AAGTGCGGTG GTATCGACAA GCGTACCATC
    GAGAAGTTCG AGAAGGAAGC CGCTGAGCTC GGTAAGGGTT CCTTCAAGTA TGCCTGGGTT
    CTTGACAAGC TCAAGGCCGA GCGTGAGCGT GGTATCACCA TCGATATCGC CCTCTGGAAG
    TTCGAGACTC CCAAGTACTA CGTCACCGTC ATCGATGCCC CCGGTCATCG TGATTTCATC
    AAGAACATGA TCACTGGTAC CTCCCAGGCT GATTGCGCTA TCCTCATCAT TGCCGCCGGT
    ACTGGTGAGT TCGAGGCTGG TATCTCCAAG GATGGCCAGA CCCGTGAGCA CGCTCTGCTC
    GCCTACACCC TCGGTGTCAA GCAGCTCATT GTTGCCATCA ACAAGATGGA CACCACCCAG
    TGGTCCCAGA CTCGTTTCGA GGAGATCATC AAGGAGACCA AGAACTTCAT CAAGAAGGTT
    GGCTACAACC CCGCTGGTGT CGCTTTCGTC CCCATCTCCG GCTTCAACGG CGACAACATG
    CTTGAGCCCT CCACCAACTG CCCCTGGTAC AAGGGTTGGG AGAAGGAGAC CAAGGCCGGC
    AAGGCCACTG GCAAGACCCT CCTCGAGGCC ATCGACGCCA TTGAGCCCCC CAAGCGTCCT
    ACCGACAAGC CCCTCCGTCT TCCCCTCCAG GATGTCTACA AGATCGGTGG TATCGGCACA
    GTGCCCGTCG GCCGTATCGA GACTGGTGTC CTCAAGCCCG GTATGGTCGT TACCTTCGCT
    CCTTCCAACG TCACCACTGA AGTCAAGTCC GTCGAGATGC ACCACGAGCA GCTTGCTCAG
    GGTGTCCCCG GTGACAACGT CGGCTTCAAC GTGAAGAACG TTTCCGTCAA GGATATCCGC
    CGTGGTAACG TTGCCGGTGA CTCCAAGAAC GACCCCCCTG CTGGCGCCGC CTCTTTCACC
    GCCCAGGTCA TCGTTCTCAA CCACCCCGGT CAGGTCGGTG CCGGCTACGC CCCCGTCCTC
    GACTGCCACA CTGCCCACAT TGCCTGCAAG TTCGCCGAGC TCCTCGAGAA GATCGACCGC
    CGTACTGGTA AGGCTGTTGA GGCCTCCCCC AAGTTCATCA AGTCTGGTGA TGCTGCCATC
    GTCAAGATGA TTCCCTCCAA GCCCATGTGC GTTGAGGCTT TCACCGACTA CCCTCCCCTC
    GGCCGTTTCG CCGTCCGTGA CATGCGTCAG ACCGTCGCCG TCGGTGTCAT CAAGGCCGTC
    GACAAGTCCA CCGCTGCCGC TGGCAAGGTC ACCAAGTCCG CTGCCAAGGC CGCCAAGAAG
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_964868.2
    CDS197..1579
    Translation

    Target ORF information:

    RefSeq Version XM_959775.3
    Organism Neurospora crassa OR74A
    Definition Neurospora crassa OR74A elongation factor 1 alpha (tef-1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_959775.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGGGCAAGG AGGACAAGAC TCACATCAAC GTCGTCGTTA TCGGCCACGT CGATTCCGGC 
    AAGTCTACCA CTACCGGTCA CTTGATCTAC AAGTGCGGTG GTATCGACAA GCGTACCATC
    GAGAAGTTCG AGAAGGAAGC CGCTGAGCTC GGTAAGGGTT CCTTCAAGTA TGCCTGGGTT
    CTTGACAAGC TCAAGGCCGA GCGTGAGCGT GGTATCACCA TCGATATCGC CCTCTGGAAG
    TTCGAGACTC CCAAGTACTA CGTCACCGTC ATCGATGCCC CCGGTCATCG TGATTTCATC
    AAGAACATGA TCACTGGTAC CTCCCAGGCT GATTGCGCTA TCCTCATCAT TGCCGCCGGT
    ACTGGTGAGT TCGAGGCTGG TATCTCCAAG GATGGCCAGA CCCGTGAGCA CGCTCTGCTC
    GCCTACACCC TCGGTGTCAA GCAGCTCATT GTTGCCATCA ACAAGATGGA CACCACCCAG
    TGGTCCCAGA CTCGTTTCGA GGAGATCATC AAGGAGACCA AGAACTTCAT CAAGAAGGTT
    GGCTACAACC CCGCTGGTGT CGCTTTCGTC CCCATCTCCG GCTTCAACGG CGACAACATG
    CTTGAGCCCT CCACCAACTG CCCCTGGTAC AAGGGTTGGG AGAAGGAGAC CAAGGCCGGC
    AAGGCCACTG GCAAGACCCT CCTCGAGGCC ATCGACGCCA TTGAGCCCCC CAAGCGTCCT
    ACCGACAAGC CCCTCCGTCT TCCCCTCCAG GATGTCTACA AGATCGGTGG TATCGGCACA
    GTGCCCGTCG GCCGTATCGA GACTGGTGTC CTCAAGCCCG GTATGGTCGT TACCTTCGCT
    CCTTCCAACG TCACCACTGA AGTCAAGTCC GTCGAGATGC ACCACGAGCA GCTTGCTCAG
    GGTGTCCCCG GTGACAACGT CGGCTTCAAC GTGAAGAACG TTTCCGTCAA GGATATCCGC
    CGTGGTAACG TTGCCGGTGA CTCCAAGAAC GACCCCCCTG CTGGCGCCGC CTCTTTCACC
    GCCCAGGTCA TCGTTCTCAA CCACCCCGGT CAGGTCGGTG CCGGCTACGC CCCCGTCCTC
    GACTGCCACA CTGCCCACAT TGCCTGCAAG TTCGCCGAGC TCCTCGAGAA GATCGACCGC
    CGTACTGGTA AGGCTGTTGA GGCCTCCCCC AAGTTCATCA AGTCTGGTGA TGCTGCCATC
    GTCAAGATGA TTCCCTCCAA GCCCATGTGC GTTGAGGCTT TCACCGACTA CCCTCCCCTC
    GGCCGTTTCG CCGTCCGTGA CATGCGTCAG ACCGTCGCCG TCGGTGTCAT CAAGGCCGTC
    GACAAGTCCA CCGCTGCCGC TGGCAAGGTC ACCAAGTCCG CTGCCAAGGC CGCCAAGAAG
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The genome sequence of the filamentous fungus Neurospora crassa.
    Nature422(6934)859-68(2003 Apr)
    Galagan JE,Calvo SE,Borkovich KA,Selker EU,Read ND,Jaffe D,FitzHugh W,Ma LJ,Smirnov S,Purcell S,Rehman B,Elkins T,Engels R,Wang S,Nielsen CB,Butler J,Endrizzi M,Qui D,Ianakiev P,Bell-Pedersen D,Nelson MA,Werner-Washburne M,Selitrennikoff CP,Kinsey JA,Braun EL,Zelter A,Schulte U,Kothe GO,Jedd G,Mewes W,Staben C,Marcotte E,Greenberg D,Roy A,Foley K,Naylor J,Stange-Thomann N,Barrett R,Gnerre S,Kamal M,Kamvysselis M,Mauceli E,Bielke C,Rudd S,Frishman D,Krystofova S,Rasmussen C,Metzenberg RL,Perkins DD,Kroken S,Cogoni C,Macino G,Catcheside D,Li W,Pratt RJ,Osmani SA,DeSouza CP,Glass L,Orbach MJ,Berglund JA,Voelker R,Yarden O,Plamann M,Seiler S,Dunlap J,Radford A,Aramayo R,Natvig DO,Alex LA,Mannhaupt G,Ebbole DJ,Freitag M,Paulsen I,Sachs MS,Lander ES,Nusbaum C,Birren B