NCU09254 cDNA ORF clone, Neurospora crassa OR74A

The following NCU09254 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NCU09254 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ONf07999 XM_959596.2
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Neurospora crassa OR74A pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16 (NCU09254), mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ONf07999
    Clone ID Related Accession (Same CDS sequence) XM_959596.2
    Accession Version XM_959596.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2523bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-10
    Organism Neurospora crassa OR74A
    Product pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026501). On Feb 23, 2015 this sequence version replaced XM_959596.1.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGTCACGCA AAAGAAAAGC CGAATCCGGC GAAGTCCTCA AAATCGGCAA CAAAACCATC 
    TCCCTCGAAG GCTACCTCAC CAAGAAACCA AAAACTGCCA GCACCCCTTC AACCTCGACA
    TCAGACAACA ACACCCCAGC ACCATCAACC GAAAATGATA CAACGACGAC AGCAACAGAC
    CCCAAGGAGT TCAAGCCCTC TCGCCCCCTC ACAGGAGATC CCTCCCTGTT CGCCACCCGC
    CGCGCCCTCC CGCTCTACCT CTACCAAGAC CGGATCCGCT CCATCCTCTC GAAAAAGGAC
    GTCCTGGTAC TAGTGGGTGA GACCGGTTCG GGTAAATCTA CCCAGGTCCC GCAGTTCTTG
    TACCAGGAGC GGTGGTGTCA GCGACGGAGG GTCAAGGTTA AGACCAAGGT GTTGGATAAA
    GATGGGAATG AGGTTCTGGA GGAGGGTGGT AAAGCAAAGA CCAGGGAGGA GACGGTTAAT
    GTCGGTGGAG TGATTGCGAT TACCCAGCCG AGAAGGGTAG CTGCTACCAC GCTTGCGGGG
    CGTGTGGCGA AGGAGTGCGG GACGCCGTTG GATACGAGTG GGAGGGGGAC AGGGTTCAAG
    GGACGGGGAC CGAAAGGGAA GGATGGAAAG GGACCGAAAG GTGGGAAGGA GGGAGAGGAG
    TGGGATGAGG AGAAGGATGT GGTGGAGGAG ATGGGGAAGA ATAATGGTGG TGATGAGGAG
    GTGATTGAGG AGAATGGGAA TGAGAAGAAG GACAGGAAAG ACCAGGAGAA GGGTCTGGTT
    GGTTATTCAG TCCGTTTCGA CCATCGAGTA CCACCGGGGA CGAAGATCAA GTACGTGACG
    GAGGGTATGT TGCTGCAGGA ACTGCTGAGG GACCCGCATC TACGACAATA CTCGGCCATC
    ATCGTGGACG AAATCCACGA GCGCAGTGTG GATGTCGATT TACTATCTGG TTTCCTCAAG
    CAGATCGTCA CGGGCGACAA GGCCGGTCGT GGAGGAATCC CGCTCAAGGT GGTGGTCATG
    AGTGCTACGG CACAGGTCGA AAGAATCAAG AAATTCTTTT CTAGCGACGA CAGTCTTAAG
    AAGGGAACAT CCGGGGAGGA AGTGGAATAT CTCGAGATCG AAGGCCGACA GTTCCCGGTC
    GAAGTCATCC ACGAGCCCAA ACCTGTTCCA GACATCCAGG AGGCACTCCT CAAAACCGTC
    TTCAAGATCC ACACCCAAGA GCCGCTCTCC GACAAACACG GCAAGAAGGA CATCCTGTGT
    TTCTTGACAG GTCAAGAAGA AATCGAAGCC GCCCAGCGCC TAATCGAAGA ATTCGCCGAA
    ACCCTCGACC CCAAAGTGCC CAAAGTCAAA GTCTTTCCCC TCTTCGGCCA ACTCTCCATG
    GAAGCCCAGC ACGAAGCCTT CCAGCCCATC AAACACCCCT TCACGCGCAA GATCGTGCTA
    GCCACCAACA TTGCCGAAAC CTCCGTCACC GTCCCCGGCG TGCGCTACGT CGTCGACTGC
    GGCAAAGCCA AAGTCAAACA ATACCGTCCC CGTCTCGGCA TGGAATCGCT GCTCGCAAAA
    CCCATCTCCA AATCCTCCGC CATCCAACGA ACCGGTCGTG CCGGCCGTGA AGGCCCCGGC
    AAGTGCTTCC GTCTCTACAC AGCCGACACC TTCGCCACCC TGCCAAAGAC GGACCTCCCC
    GAGATTCTGC GGACCGACGT CCTCGGCGCC GTCCTGACAA TGAAAGCCCG CGGCATCGAC
    GACGTGCTCT CCTTCCCGTT GATGGACCGT CCGGAACTCG AAGCCGTCGA AAACGCTCTT
    GTACATCTAC ATGTGCTCGG CGCGCTAGGC GACGACGGCC GCATCACCGA CACCGGCCGC
    AAAATGGTCT TGTTCCCCGT GACTGCGCCC TACGCGCGCG TCCTGCTCGC GGCCGCTGAC
    CCGCGCTATG ACTGTCTGCT GGAAGCCATC GACATCATTT CGTGCATCAC GGCCGGCGAC
    GACATCTTCA TGCAAGTCCG CTCGGAAGAA GACAAGGAAG CCGTGGACGT GTACAGAAAA
    GAACTCCAGC GCCGCGAGGG CGATTTGATC ACGTACCTGA ACACCATGCA ACGCTTCGTT
    TCCACGCCCT CAGCCGACCG TGGGACGTTC TGCAAACAAC GCCGGATCAA TGTGCGGAAT
    ATGCGGCAGG CGCTGAATAT CAGGAAACAA CTCCGGTCGC TGTCGGTCAA GAATAAGATG
    CTGGCGGAGT TTCCAGCCCC AGATGCGGAA GTGACGTACC AGCCTGTGTC GCCGGAGACG
    GCAGAGAGGA TCCTCAAGGC GTTCTTGAAG GGGTTCGCGA TGAAGACGGC GGTGCTGGCG
    CCCGATGCGA GTTATGTGAC TGCCCATGGG AAACATGTGG TTGCTATTCA CCCGAGTAGT
    AGTTTGCATG GGGTCAAGAG GGAGGCCATC ATGTTTTTGG AGCATGTGTA TACGCAAAAG
    AATTATGCAA AGAAGGTGAG CGCCATTCAG GCGGGGTGGA TTGCGGAGGC GTTGGCTGTT
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_964689.1
    CDS70..2592
    Translation

    Target ORF information:

    RefSeq Version XM_959596.2
    Organism Neurospora crassa OR74A
    Definition Neurospora crassa OR74A pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16 (NCU09254), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_959596.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGTCACGCA AAAGAAAAGC CGAATCCGGC GAAGTCCTCA AAATCGGCAA CAAAACCATC 
    TCCCTCGAAG GCTACCTCAC CAAGAAACCA AAAACTGCCA GCACCCCTTC AACCTCGACA
    TCAGACAACA ACACCCCAGC ACCATCAACC GAAAATGATA CAACGACGAC AGCAACAGAC
    CCCAAGGAGT TCAAGCCCTC TCGCCCCCTC ACAGGAGATC CCTCCCTGTT CGCCACCCGC
    CGCGCCCTCC CGCTCTACCT CTACCAAGAC CGGATCCGCT CCATCCTCTC GAAAAAGGAC
    GTCCTGGTAC TAGTGGGTGA GACCGGTTCG GGTAAATCTA CCCAGGTCCC GCAGTTCTTG
    TACCAGGAGC GGTGGTGTCA GCGACGGAGG GTCAAGGTTA AGACCAAGGT GTTGGATAAA
    GATGGGAATG AGGTTCTGGA GGAGGGTGGT AAAGCAAAGA CCAGGGAGGA GACGGTTAAT
    GTCGGTGGAG TGATTGCGAT TACCCAGCCG AGAAGGGTAG CTGCTACCAC GCTTGCGGGG
    CGTGTGGCGA AGGAGTGCGG GACGCCGTTG GATACGAGTG GGAGGGGGAC AGGGTTCAAG
    GGACGGGGAC CGAAAGGGAA GGATGGAAAG GGACCGAAAG GTGGGAAGGA GGGAGAGGAG
    TGGGATGAGG AGAAGGATGT GGTGGAGGAG ATGGGGAAGA ATAATGGTGG TGATGAGGAG
    GTGATTGAGG AGAATGGGAA TGAGAAGAAG GACAGGAAAG ACCAGGAGAA GGGTCTGGTT
    GGTTATTCAG TCCGTTTCGA CCATCGAGTA CCACCGGGGA CGAAGATCAA GTACGTGACG
    GAGGGTATGT TGCTGCAGGA ACTGCTGAGG GACCCGCATC TACGACAATA CTCGGCCATC
    ATCGTGGACG AAATCCACGA GCGCAGTGTG GATGTCGATT TACTATCTGG TTTCCTCAAG
    CAGATCGTCA CGGGCGACAA GGCCGGTCGT GGAGGAATCC CGCTCAAGGT GGTGGTCATG
    AGTGCTACGG CACAGGTCGA AAGAATCAAG AAATTCTTTT CTAGCGACGA CAGTCTTAAG
    AAGGGAACAT CCGGGGAGGA AGTGGAATAT CTCGAGATCG AAGGCCGACA GTTCCCGGTC
    GAAGTCATCC ACGAGCCCAA ACCTGTTCCA GACATCCAGG AGGCACTCCT CAAAACCGTC
    TTCAAGATCC ACACCCAAGA GCCGCTCTCC GACAAACACG GCAAGAAGGA CATCCTGTGT
    TTCTTGACAG GTCAAGAAGA AATCGAAGCC GCCCAGCGCC TAATCGAAGA ATTCGCCGAA
    ACCCTCGACC CCAAAGTGCC CAAAGTCAAA GTCTTTCCCC TCTTCGGCCA ACTCTCCATG
    GAAGCCCAGC ACGAAGCCTT CCAGCCCATC AAACACCCCT TCACGCGCAA GATCGTGCTA
    GCCACCAACA TTGCCGAAAC CTCCGTCACC GTCCCCGGCG TGCGCTACGT CGTCGACTGC
    GGCAAAGCCA AAGTCAAACA ATACCGTCCC CGTCTCGGCA TGGAATCGCT GCTCGCAAAA
    CCCATCTCCA AATCCTCCGC CATCCAACGA ACCGGTCGTG CCGGCCGTGA AGGCCCCGGC
    AAGTGCTTCC GTCTCTACAC AGCCGACACC TTCGCCACCC TGCCAAAGAC GGACCTCCCC
    GAGATTCTGC GGACCGACGT CCTCGGCGCC GTCCTGACAA TGAAAGCCCG CGGCATCGAC
    GACGTGCTCT CCTTCCCGTT GATGGACCGT CCGGAACTCG AAGCCGTCGA AAACGCTCTT
    GTACATCTAC ATGTGCTCGG CGCGCTAGGC GACGACGGCC GCATCACCGA CACCGGCCGC
    AAAATGGTCT TGTTCCCCGT GACTGCGCCC TACGCGCGCG TCCTGCTCGC GGCCGCTGAC
    CCGCGCTATG ACTGTCTGCT GGAAGCCATC GACATCATTT CGTGCATCAC GGCCGGCGAC
    GACATCTTCA TGCAAGTCCG CTCGGAAGAA GACAAGGAAG CCGTGGACGT GTACAGAAAA
    GAACTCCAGC GCCGCGAGGG CGATTTGATC ACGTACCTGA ACACCATGCA ACGCTTCGTT
    TCCACGCCCT CAGCCGACCG TGGGACGTTC TGCAAACAAC GCCGGATCAA TGTGCGGAAT
    ATGCGGCAGG CGCTGAATAT CAGGAAACAA CTCCGGTCGC TGTCGGTCAA GAATAAGATG
    CTGGCGGAGT TTCCAGCCCC AGATGCGGAA GTGACGTACC AGCCTGTGTC GCCGGAGACG
    GCAGAGAGGA TCCTCAAGGC GTTCTTGAAG GGGTTCGCGA TGAAGACGGC GGTGCTGGCG
    CCCGATGCGA GTTATGTGAC TGCCCATGGG AAACATGTGG TTGCTATTCA CCCGAGTAGT
    AGTTTGCATG GGGTCAAGAG GGAGGCCATC ATGTTTTTGG AGCATGTGTA TACGCAAAAG
    AATTATGCAA AGAAGGTGAG CGCCATTCAG GCGGGGTGGA TTGCGGAGGC GTTGGCTGTT
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The genome sequence of the filamentous fungus Neurospora crassa.
    Nature422(6934)859-68(2003 Apr)
    Galagan JE,Calvo SE,Borkovich KA,Selker EU,Read ND,Jaffe D,FitzHugh W,Ma LJ,Smirnov S,Purcell S,Rehman B,Elkins T,Engels R,Wang S,Nielsen CB,Butler J,Endrizzi M,Qui D,Ianakiev P,Bell-Pedersen D,Nelson MA,Werner-Washburne M,Selitrennikoff CP,Kinsey JA,Braun EL,Zelter A,Schulte U,Kothe GO,Jedd G,Mewes W,Staben C,Marcotte E,Greenberg D,Roy A,Foley K,Naylor J,Stange-Thomann N,Barrett R,Gnerre S,Kamal M,Kamvysselis M,Mauceli E,Bielke C,Rudd S,Frishman D,Krystofova S,Rasmussen C,Metzenberg RL,Perkins DD,Kroken S,Cogoni C,Macino G,Catcheside D,Li W,Pratt RJ,Osmani SA,DeSouza CP,Glass L,Orbach MJ,Berglund JA,Voelker R,Yarden O,Plamann M,Seiler S,Dunlap J,Radford A,Aramayo R,Natvig DO,Alex LA,Mannhaupt G,Ebbole DJ,Freitag M,Paulsen I,Sachs MS,Lander ES,Nusbaum C,Birren B