NCU00517 cDNA ORF clone, Neurospora crassa OR74A

Gene
Clones
gRNAs

Summary

Gene Symbol	NCU00517
Entrez Gene ID	3880461
Full Name	cell cycle checkpoint protein RAD17
Gene Type	protein-coding
Organism	Neurospora crassa OR74A

mRNA and Protein(s)

mRNA	Protein	Name
XM_959228.2	XP_964321.1	cell cycle checkpoint protein RAD17

Pathways from BioSystems

Homologies

Schizosaccharomyces pombe (fission yeast)	rad17	NP_594918.1
Magnaporthe oryzae (rice blast fungus)	MGG_15566	XP_003713691.1
Neurospora crassa	NCU00517	XP_964321.1

Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Galagan JE, Calvo SE, Borkovich KA, Selker EU, Read ND, Jaffe D, FitzHugh W, Ma LJ, Smirnov S, Purcell S, Rehman B, Elkins T, Engels R, Wang S, Nielsen CB, Butler J, Endrizzi M, Qui D, Ianakiev P, Bell-Pedersen D, Nelson MA, Werner-Washburne M, Selitrennikoff CP, Kinsey JA, Braun EL, Zelter A, Schulte U, Kothe GO, Jedd G, Mewes W, Staben C, Marcotte E, Greenberg D, Roy A, Foley K, Naylor J, Stange-Thomann N, Barrett R, Gnerre S, Kamal M, Kamvysselis M, Mauceli E, Bielke C, Rudd S, Frishman D, Krystofova S, Rasmussen C, Metzenberg RL, Perkins DD, Kroken S, Cogoni C, Macino G, Catcheside D, Li W, Pratt RJ, Osmani SA, DeSouza CP, Glass L, Orbach MJ, Berglund JA, Voelker R, Yarden O, Plamann M, Seiler S, Dunlap J, Radford A, Aramayo R, Natvig DO, Alex LA, Mannhaupt G, Ebbole DJ, Freitag M, Paulsen I, Sachs MS, Lander ES, Nusbaum C, Birren B
Nature422(6934)859-68(2003 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following NCU00517 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NCU00517 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ONf02745	XM_959228.2 Latest version!	Neurospora crassa OR74A cell cycle checkpoint protein RAD17 (NCU00517), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NCU00517 ( XM_959228.2 ) cDNA ORF clone, Neurospora crassa OR74A -> XP_964321.1 Neurospora crassa OR74A cell cycle checkpoint protein RAD17 (NCU00517), mRNA.

Price & Availability↑

CloneID	ONf02745
Clone ID Related Accession (Same CDS sequence)	XM_959228.2
Accession Version	XM_959228.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2835bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-10
Organism	Neurospora crassa OR74A
Product	cell cycle checkpoint protein RAD17
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026501). On Feb 23, 2015 this sequence version replaced XM_959228.1.

ATGGCGCCGG CTGCAAAGCG ACGCAAACGG ACCATTGTCG ACGACTCTGA CGCCGAAGAA 
GACGAGAAGA AGCAGACGAG TCAAAACAAT CAAAACACCC TGAATCGCTA CCTCATCGCG 
TCCCCCGTCG CGAGCAAGTC CGCTAAGCCA GACGATAACG ACCTCCCAGA AGTAATACCA 
GAGTCTTCAA GTCCTGTGAG AGCCACTACG AGAACTACAC GCGCTTCGAC AAGATCTCAA 
CCTCCAGCTG CATCGTCTGC CACCCTTCGG AACCGACTGC CAAAAGCTGC TTCCGGGAGC 
CCAAGCGCAA GCCCGATCAA GTCACGGACG AGGGCACAGA AGAAACTCAA AACTGAGGAA 
AATGGGAGGA ATGGGGACCT CAAGGTTCTC TTCTCGAAGC AAACGGAAAG GAGTCAAGCA 
TCTGCGCAAT CGGGCGTGAG CAGCAAGGAT ATTAGCGATT TCCTTGAGGA TATCATTAGC 
GAGTCCGATG ACGACATCGC TCTGTCCAAG ACGGCAGCAT CCAGCTTGGT TGGACTGCAA 
GCTCACAGGC GCTTCAAAGA CAACTCCCAG GGCACCTCCC AAGGGAGCTC GTTGTTCAGT 
ACCCAGACTA GTACGCTAAG TTCAACCACG GGTCAGCGCT TCTTGAAGCC GTCAAGACCA 
ACTAGGCCAG TGGTTGCCCC ACCTCCGTCG ACCGATGACG ATTCCCGACC GTGGTCCGAA 
CGATTCGCCC CAACAAACCT GGAGGAGCTG GCTGTACACA AAAGGAAAGT GGGAGATGTG 
CGGAAATGGC TGGAGGACGT TATTGCTGGC CGGATGAGGC AGCGCCTCCT GGTGCTGAAG 
GGCGCTGCTG GCACGGGCAA AACGACCACT CTGAAACTGC TAGCCGAGGA TATGCGATGC 
GAAGTTCTTG AATGGAGAAA TCCAGCAAGT TCATACGGTG TTCCTCAGGG ATACCAGTCT 
GCGTCTTCGC AGTTCGAAGA GTTCTTGGGA AGAGGCCGGA AGTTTGGACA GCTGGACCTG 
GAAGGCGACT CACCTCTGCC GGCACTGGCG CCCACGATCA ACACATCAAA TCAGGGCCGA 
AGGGTAATCC TAATTGAAGA ATTTCCCAAT ACCTTCATGC GATCATCTAC CGCCTTGACC 
AACTTCAGGA ACACAATTTA TGAGTTCTTG GCGGCTAACA CCCCAGCACT CACACCCTTT 
GGGCAGACAC CACCCGCGGA CTCGATTGTT CCAATAGTAA TGGTAATATC TGAGACCTTG 
CTTACTACAA CATCTGCTTC CGCCGACAGC TTTACTGCAC ATCGATTACT AGGTCCCGAG 
ATTTTGCGAC ACCCTGGGAC AGGTGTCATC GAATTCAACC CCATTGCGCC ATCTCTCCTC 
GCTAAAGCCT TAGAGCTGGT AGTTAAGAAG GAAGCCCGAA GGTCGGGCCG CCGAATGACC 
CCTGGACCAC TTGTACTCAA ACGTCTTGGA GAGATTGGGG ACATCCGAAA TGCAATTGCA 
TCGCTAGAGT TCCTTTGTGT TAAGGGCGAC GATGACGCCG ACTGGGGATC CAAGGTTGCT 
TTCACGAAGA CGAAAAAAGG ATCAAAGGAT GCAGCGCTGA CCAAGGGTGA GCAAGAAAGC 
CTTGAGCTAA TATCTCAGCG AGAGGCAACG CTGGGTATCT TCCACGCGGT TGGAAAAGTC 
GTATACAACA AAAGGGCTGA AAAGCCGCTC CCCTTGGGGT CTGAAGAAGC AAAAGCGGAG 
ACATTACCAG GCTACCTGTC ACACTTGTCC AGGCCGAAGA AATCCGAAGT TGCTGTTGAC 
ACGCTAATCG ATGAACTGGG TACTGACACC CAGACCTTCA TTGCGGCCTT ACACGAAAAT 
TACGCCCTGT CGTGCGAGAG TAGTCCCCTC GATCCGAATT CTTCCTTAGA CTACATGAAT 
GGATGTCTCG ACTACCTGTC CGAAGCCGAC CTTCTCTGTC CCTCCTGGGA TATTTTTTTT 
GGCGGAAAGG GAGGGTTTGC CGGCGGAAAT TACGGCGGCA AGGACTCGGG TAGCCATATA 
CTCCGGCAGG ATGAAATGGC ATTCCAGGTC GCCGTTCGCG GCCTATTGTT CTCCTTACCG 
TCGCCTGTTA AACGGCAGAC GCACCCAACG TCAGGTCGTG GGGGAGGAGA CGCATTCAAA 
ATGTTCTACC CGGCTTATCT CAAGCTGTGG AGGGCCAAGG AGGAAATGGA AGGTTGTGTG 
GACATGTGGG CTACCAAGAT GCTGAAGGGC GAGGACGGCA TGACATTATC GCATAGCGGC 
GGCACAACTA TGAATTTGAC CAGCGGTGCA TCGGCGTTCC AAAGGCCATC CTCGACAGTG 
AACCCGAACG TCAACAGCGT GAAAGATTGG GCTAGCACAC AAAGACCCCA GCCAGTCAGG 
CAAGAGCCGA CCTCCGCGTC AAGTAGTTCC GCGCCCCCTC TCCTCTCGCT CGGCTCCGCC 
GCTCGACAAG AAATGGTCCT CGAGCGTCTC CCTTACATGG CACACATTGC CCGCGGTCGA 
CGCGGCTCGT TCGGTTCCAT GCGTATGCGG GACCTAGACA AGATCGTGTC CTTCCATGGC 
ATTGGGCCTC CCGCAACCGA GGATTTGGAC GAAGACGAAG CTGTAGGAGC GGAGGAGGCA 
GGGGTGACTG AGAGTTGGAC TACGGATAAA CCTGTGGAGG AAGCGATGAT CCCCGGGCGG 
GACAAGAGAG GGCTAGGTGC GATCCTCAGC CGGGCTACTG CCAGTAGGGT GCATGGTAAT 
GGACAGGGGC AGGAGGCGGC GGAGCTGGGT GGGATTCAGA GCTTGGTGTT GAGTGATGAT 
GATATCGAGG ATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_964321.1
CDS	179..3013
Translation	MAPAAKRRKRTIVDDSDAEEDEKKQTSQNNQNTLNRYLIASPVASKSAKPDDNDLPEVIPESSSPVRATTRTTRASTRSQPPAASSATLRNRLPKAASGSPSASPIKSRTRAQKKLKTEENGRNGDLKVLFSKQTERSQASAQSGVSSKDISDFLEDIISESDDDIALSKTAASSLVGLQAHRRFKDNSQGTSQGSSLFSTQTSTLSSTTGQRFLKPSRPTRPVVAPPPSTDDDSRPWSERFAPTNLEELAVHKRKVGDVRKWLEDVIAGRMRQRLLVLKGAAGTGKTTTLKLLAEDMRCEVLEWRNPASSYGVPQGYQSASSQFEEFLGRGRKFGQLDLEGDSPLPALAPTINTSNQGRRVILIEEFPNTFMRSSTALTNFRNTIYEFLAANTPALTPFGQTPPADSIVPIVMVISETLLTTTSASADSFTAHRLLGPEILRHPGTGVIEFNPIAPSLLAKALELVVKKEARRSGRRMTPGPLVLKRLGEIGDIRNAIASLEFLCVKGDDDADWGSKVAFTKTKKGSKDAALTKGEQESLELISQREATLGIFHAVGKVVYNKRAEKPLPLGSEEAKAETLPGYLSHLSRPKKSEVAVDTLIDELGTDTQTFIAALHENYALSCESSPLDPNSSLDYMNGCLDYLSEADLLCPSWDIFFGGKGGFAGGNYGGKDSGSHILRQDEMAFQVAVRGLLFSLPSPVKRQTHPTSGRGGGDAFKMFYPAYLKLWRAKEEMEGCVDMWATKMLKGEDGMTLSHSGGTTMNLTSGASAFQRPSSTVNPNVNSVKDWASTQRPQPVRQEPTSASSSSAPPLLSLGSAARQEMVLERLPYMAHIARGRRGSFGSMRMRDLDKIVSFHGIGPPATEDLDEDEAVGAEEAGVTESWTTDKPVEEAMIPGRDKRGLGAILSRATASRVHGNGQGQEAAELGGIQSLVLSDDDIED

Target ORF information:

RefSeq Version	XM_959228.2
Organism	Neurospora crassa OR74A
Definition	Neurospora crassa OR74A cell cycle checkpoint protein RAD17 (NCU00517), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_959228.2

ORF Insert Sequence:

ATGGCGCCGG CTGCAAAGCG ACGCAAACGG ACCATTGTCG ACGACTCTGA CGCCGAAGAA 
GACGAGAAGA AGCAGACGAG TCAAAACAAT CAAAACACCC TGAATCGCTA CCTCATCGCG 
TCCCCCGTCG CGAGCAAGTC CGCTAAGCCA GACGATAACG ACCTCCCAGA AGTAATACCA 
GAGTCTTCAA GTCCTGTGAG AGCCACTACG AGAACTACAC GCGCTTCGAC AAGATCTCAA 
CCTCCAGCTG CATCGTCTGC CACCCTTCGG AACCGACTGC CAAAAGCTGC TTCCGGGAGC 
CCAAGCGCAA GCCCGATCAA GTCACGGACG AGGGCACAGA AGAAACTCAA AACTGAGGAA 
AATGGGAGGA ATGGGGACCT CAAGGTTCTC TTCTCGAAGC AAACGGAAAG GAGTCAAGCA 
TCTGCGCAAT CGGGCGTGAG CAGCAAGGAT ATTAGCGATT TCCTTGAGGA TATCATTAGC 
GAGTCCGATG ACGACATCGC TCTGTCCAAG ACGGCAGCAT CCAGCTTGGT TGGACTGCAA 
GCTCACAGGC GCTTCAAAGA CAACTCCCAG GGCACCTCCC AAGGGAGCTC GTTGTTCAGT 
ACCCAGACTA GTACGCTAAG TTCAACCACG GGTCAGCGCT TCTTGAAGCC GTCAAGACCA 
ACTAGGCCAG TGGTTGCCCC ACCTCCGTCG ACCGATGACG ATTCCCGACC GTGGTCCGAA 
CGATTCGCCC CAACAAACCT GGAGGAGCTG GCTGTACACA AAAGGAAAGT GGGAGATGTG 
CGGAAATGGC TGGAGGACGT TATTGCTGGC CGGATGAGGC AGCGCCTCCT GGTGCTGAAG 
GGCGCTGCTG GCACGGGCAA AACGACCACT CTGAAACTGC TAGCCGAGGA TATGCGATGC 
GAAGTTCTTG AATGGAGAAA TCCAGCAAGT TCATACGGTG TTCCTCAGGG ATACCAGTCT 
GCGTCTTCGC AGTTCGAAGA GTTCTTGGGA AGAGGCCGGA AGTTTGGACA GCTGGACCTG 
GAAGGCGACT CACCTCTGCC GGCACTGGCG CCCACGATCA ACACATCAAA TCAGGGCCGA 
AGGGTAATCC TAATTGAAGA ATTTCCCAAT ACCTTCATGC GATCATCTAC CGCCTTGACC 
AACTTCAGGA ACACAATTTA TGAGTTCTTG GCGGCTAACA CCCCAGCACT CACACCCTTT 
GGGCAGACAC CACCCGCGGA CTCGATTGTT CCAATAGTAA TGGTAATATC TGAGACCTTG 
CTTACTACAA CATCTGCTTC CGCCGACAGC TTTACTGCAC ATCGATTACT AGGTCCCGAG 
ATTTTGCGAC ACCCTGGGAC AGGTGTCATC GAATTCAACC CCATTGCGCC ATCTCTCCTC 
GCTAAAGCCT TAGAGCTGGT AGTTAAGAAG GAAGCCCGAA GGTCGGGCCG CCGAATGACC 
CCTGGACCAC TTGTACTCAA ACGTCTTGGA GAGATTGGGG ACATCCGAAA TGCAATTGCA 
TCGCTAGAGT TCCTTTGTGT TAAGGGCGAC GATGACGCCG ACTGGGGATC CAAGGTTGCT 
TTCACGAAGA CGAAAAAAGG ATCAAAGGAT GCAGCGCTGA CCAAGGGTGA GCAAGAAAGC 
CTTGAGCTAA TATCTCAGCG AGAGGCAACG CTGGGTATCT TCCACGCGGT TGGAAAAGTC 
GTATACAACA AAAGGGCTGA AAAGCCGCTC CCCTTGGGGT CTGAAGAAGC AAAAGCGGAG 
ACATTACCAG GCTACCTGTC ACACTTGTCC AGGCCGAAGA AATCCGAAGT TGCTGTTGAC 
ACGCTAATCG ATGAACTGGG TACTGACACC CAGACCTTCA TTGCGGCCTT ACACGAAAAT 
TACGCCCTGT CGTGCGAGAG TAGTCCCCTC GATCCGAATT CTTCCTTAGA CTACATGAAT 
GGATGTCTCG ACTACCTGTC CGAAGCCGAC CTTCTCTGTC CCTCCTGGGA TATTTTTTTT 
GGCGGAAAGG GAGGGTTTGC CGGCGGAAAT TACGGCGGCA AGGACTCGGG TAGCCATATA 
CTCCGGCAGG ATGAAATGGC ATTCCAGGTC GCCGTTCGCG GCCTATTGTT CTCCTTACCG 
TCGCCTGTTA AACGGCAGAC GCACCCAACG TCAGGTCGTG GGGGAGGAGA CGCATTCAAA 
ATGTTCTACC CGGCTTATCT CAAGCTGTGG AGGGCCAAGG AGGAAATGGA AGGTTGTGTG 
GACATGTGGG CTACCAAGAT GCTGAAGGGC GAGGACGGCA TGACATTATC GCATAGCGGC 
GGCACAACTA TGAATTTGAC CAGCGGTGCA TCGGCGTTCC AAAGGCCATC CTCGACAGTG 
AACCCGAACG TCAACAGCGT GAAAGATTGG GCTAGCACAC AAAGACCCCA GCCAGTCAGG 
CAAGAGCCGA CCTCCGCGTC AAGTAGTTCC GCGCCCCCTC TCCTCTCGCT CGGCTCCGCC 
GCTCGACAAG AAATGGTCCT CGAGCGTCTC CCTTACATGG CACACATTGC CCGCGGTCGA 
CGCGGCTCGT TCGGTTCCAT GCGTATGCGG GACCTAGACA AGATCGTGTC CTTCCATGGC 
ATTGGGCCTC CCGCAACCGA GGATTTGGAC GAAGACGAAG CTGTAGGAGC GGAGGAGGCA 
GGGGTGACTG AGAGTTGGAC TACGGATAAA CCTGTGGAGG AAGCGATGAT CCCCGGGCGG 
GACAAGAGAG GGCTAGGTGC GATCCTCAGC CGGGCTACTG CCAGTAGGGT GCATGGTAAT 
GGACAGGGGC AGGAGGCGGC GGAGCTGGGT GGGATTCAGA GCTTGGTGTT GAGTGATGAT 
GATATCGAGG ATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Nature422(6934)859-68(2003 Apr)
Galagan JE,Calvo SE,Borkovich KA,Selker EU,Read ND,Jaffe D,FitzHugh W,Ma LJ,Smirnov S,Purcell S,Rehman B,Elkins T,Engels R,Wang S,Nielsen CB,Butler J,Endrizzi M,Qui D,Ianakiev P,Bell-Pedersen D,Nelson MA,Werner-Washburne M,Selitrennikoff CP,Kinsey JA,Braun EL,Zelter A,Schulte U,Kothe GO,Jedd G,Mewes W,Staben C,Marcotte E,Greenberg D,Roy A,Foley K,Naylor J,Stange-Thomann N,Barrett R,Gnerre S,Kamal M,Kamvysselis M,Mauceli E,Bielke C,Rudd S,Frishman D,Krystofova S,Rasmussen C,Metzenberg RL,Perkins DD,Kroken S,Cogoni C,Macino G,Catcheside D,Li W,Pratt RJ,Osmani SA,DeSouza CP,Glass L,Orbach MJ,Berglund JA,Voelker R,Yarden O,Plamann M,Seiler S,Dunlap J,Radford A,Aramayo R,Natvig DO,Alex LA,Mannhaupt G,Ebbole DJ,Freitag M,Paulsen I,Sachs MS,Lander ES,Nusbaum C,Birren B

見積依頼

Eメール

japanmarket@genscript.com

電話

03-6811-6572

FAX

03-6811-6573
050-3174-7589(受注専用)

オンラインフォーム

オンライン注文

NCU00517 cDNA ORF clone, Neurospora crassa OR74A

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

会社情報

サポート

会社情報