NCU06365 cDNA ORF clone, Neurospora crassa OR74A

The following NCU06365 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NCU06365 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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ONf01090 XM_957306.2
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Neurospora crassa OR74A DEAD/DEAH box helicase (NCU06365), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ONf01090
    Clone ID Related Accession (Same CDS sequence) XM_957306.2
    Accession Version XM_957306.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3528bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-10
    Organism Neurospora crassa OR74A
    Product DEAD/DEAH box helicase
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026504). On Feb 23, 2015 this sequence version replaced XM_957306.1.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCTTCCA GCCACCATAG CGCCAATGCT ACTACTACTG ATCCACTGAA AGGGCTCAAG 
    CGCAAACGCC TGTCCACCGA ACCCGAAGCG TCGACGGAGC CGAGGCACGA TGCCGCTTTC
    GCCGGAAATC CTAGCTGCGA CTCCCAGGAG AGTTCCGGTA GTTGTGGCCC AGCAACAACG
    GGGCCAAAGA AGCGGCGAGG TCGTCCCCCC AAGCTTGTTT CCGAGCCTAC CATTGCCGAA
    AGCGATATCG AATGGCCGGC GTGGTTCAAG GAACTCGAGA AGACGCACAG GGCTTTGAAC
    CTCGTCTACA CCTTTTGCTC TACCCGAAAG CATCTGGCGA CTACGTTTGA GACGATACGG
    TCCACGGTTG AGTCACACAC AAAGCGTTCA TTAACGATTG AGGATGTAGC TGCTATTGTA
    GCCTTGCGTC CGGAGAGCAT TCAGTTTGCC TATGTCGATG AGCTAATGCT ACAGCTGGAT
    GCGAGGGGTG CCGAAAAGGA TGAGGCCTTC AAGACAGGAA GGTATGCTGG GAAGTCATCT
    CAGTCCTTTG TACCCGACGC GTCTGTTGGC GGTATGACCG GCTTGGAAGG ACTAGGTGAT
    GCTGGCCCGG ACCATACCGG TGAAGATGAG CAAGAGTCGA AATCTGGTCG GGAAGTCCTC
    TACTTTGAGT TCATTGATGA AGATCTCAAG CGGCAAGTCC AGGACAAGAA AACAGGAGTC
    CCGACTAAGC CGACTCGCAG GCTTCGACAT GAGCATGTCA AAATGCCTGT GTATAGTCAG
    AAGCAAATGA CGGCACTAAT AGAAAAGAGG AACACCAAGT TCGCCAATGC AGTCAACATT
    TTCTTGAACC GCTGTGCTGC AGAAAGTCTA GATCCGGAAA TGGCACTCAG AAGCGAGTCC
    GAACCCTTCG TTCCCAGGCC CTCAACACCC AGAGCGTCAA CCCCCCAGAC AAAACCCAGC
    ACTTTGCCCG CAAGCATCCC GAAGGAGAGG AAAGGTATTC CGGAGATAGT ACAGGAGCTG
    AAGGATAGTC CCTGGTACAC GGGTCAGATA GTCCCCGATG GCCATCGCGT GAACGAAGCT
    CAGGAACCTG TTTACGGTTA TCTGAATTTT CTACTCAGCC AAGATCTGGT TAATGCGCTT
    TACAACACCA AGGGAATTAC CCAGTTTTAT GCACATCAAG CTGATGCCAT TAACGCTCTA
    CACGACGGCC GTCACGTGGT AGTGTCCACG TCCACCAGCT CGGGAAAGTC CCTCATCTAT
    CAATTGCCTG TCATACATGC CCTTGAGCAG GACCGGAATA CGCGAGCCAT GTACATCTTC
    CCAACTAAGG CGCTTGCGCA AGACCAGAAG CGGAGCCTGA AGGAAGTCAT GAGCCTTATG
    CCTGGACTGG AAGAGATGAT CGTTGAAACA TTTGACGGCG ATACGCCAAT GCATGACAGA
    AACATCATCC GCGATGATGC ACGCATCATC TTCACGAATC CTGATATGCT GCATATCACG
    ATCTTACCCC AGGAGGAAAG GTGGAGGTCG TTTCTGCAAA ACCTGAAATA TGTTGTTGTG
    GATGAATTGC ATTATTACAA CGGCCTAATG GGTTCACATG TTGCCTTCAT CATGAGACGA
    CTGCGCCGAA TCCTCGCAGC GTTGGGAAAT ACGCATGTAC TCTTCATCTC GTGTTCAGCA
    ACCGTGGCCA ACCCAAAGGA GCATTTCACT ACCATCTTCG GCGTTGAAGA CGTCAAACTC
    ATTGACTTCA ACGGCTCTCC GTCGGGTCGC AAGGAGTTCC TCTGCTGGAA CACTCCGTAC
    AAAGACCCTG GAGATCCGTC CAGCGGACGT GGCAATGCCA AGTTGGAGTG TTCCCGCCTG
    TTCTGCGAGC TCATCCTCCG CGGCGTTCGT GTCATCGCAT TCTGTCGCGT ACGCGAACAC
    TGCGAAAAAC TGGTCAATGC AATCAAACAA GAACTGGAGC ACAGAGGCCG CTCCGAGTGC
    ATGTCTCGAG TCATGGGGTA CCGAGGCGGT TACACTGCCC AGGACAGGCG CCGTATAGAA
    ACTGAAATGT TTGAGGGCAA GCTCATGGGC ATCGTGGCGA CCACCGCTCT TGAAATTGGC
    ATTGACATCG GAAATCTAGA TTGTGTCATA ACTTGGGGGT TTCCGTACAC AATAGCGAAC
    CTCAGACAGC AGAGTGGAAG AGCTGGCCGG CGAAATAGAG ACTCTCTGTC GATTCTTCTA
    GGAGACAGTT TCGCAACCGA CCAGTATTAC ATGCAGAATC CGGATGAACT ATTCACAAAA
    CCGAACTGTG AGCTTAGTAT AGATATGGAT AACATGTTGG TCAAGGAGGG CCACATTCAA
    TGCGCGGCAC ACGAAATGCC AATTCTACCG GCTAAGGATT CCAAATACTT TGGTGACGAT
    CTAGCAACGG TTTGCGAAGA GCGGCTGCTC AAGGGTGATC GGGGCTTTTA CCATTGTCAT
    GATAAGTTTC GACCCAGGCC GTCACAGTTT GTATCCATAC GCGATATCGA AGAAGACCAT
    TTCGCCATCG TCGACGTCAC GAACAACCGG AACGTTGTCC TAGAGGAGTT GGAGGCATCC
    CGAGCGACCT TCACCATCTA CGACGGAGCC ATATTCCTAC ACCAAGGTAA CACCTACCTC
    GTACGGGACT TCAACCCCGA CAATAAGATG GCGCGAGTCG AGAAGGTCAA GGTGGACTGG
    ACCACTCAGC AGCGGGATTA CACGGACATT GACCCGGTGG AGACAGAAGC CATCCGGCGC
    ATCAAAGGAT CGAAATCCCT CGCCTATCAT GGGGTCATCA GAATCACCCA GGTTGTCTTT
    GGCTACTTCA AAGTCGACAA AAAGAATAGA ATACTTGACG CCATCCAAGT TGACAACCCG
    CCCGTTATCC GCTACAGTAA GGGCACCTGG CTCGACGTTC CGAAATCTTC ACTCGCAATC
    CTTACGTCGC GGCGCCTTCA CGTTGGCGGA GCCATCCACG CCGCCCAGCA CGCCATCATG
    AGCCTCATGC CCAACTTCGT CATGAGCATG CCGGGCGACG TGCGCACCGA GTGCAAGAAC
    CACCTCAAAG AGTTTGCCAA GCGGGAGACG CAGCGGAAAC GGCCGGCTCG CCTGACCTTC
    TACGACGCAA AGGGGGGCGC AGGCGGGTCG GGCATCAACA TCAAGGCGTT CGAGTTTATC
    GACACCTTAC TGAGGAAGGC GCTCGGTCGG GTGTTGGCAT GTCAGTGCCG CGAGGGTTGC
    ATCGAGTGCG TGTGCTCAGA GCTCTGCAAG GAGGCTAATT CGGTTATCAG CAAGGCGGGA
    GCTGAGGTGA TACTCAAGAG CTTGTTGGAT GAGGAGATTG ATGTGGAGAG CTTGCCAATG
    GGGCCGGAGG AGATCAGTCC GGCGGGCGTG GAAACGGTGA TTTTGGCGAA ACCGGTGCCG
    CCGCGGAACA GGAAGACGGT CAAGATTATT GGGCTTGCGG GGGAGGAGAT TGAGATTCAG
    GTCAATGATG AGCAGGATGA TGGGGAAGAG GAGGTAGATG GTTCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_962399.1
    CDS239..3766
    Translation

    Target ORF information:

    RefSeq Version XM_957306.2
    Organism Neurospora crassa OR74A
    Definition Neurospora crassa OR74A DEAD/DEAH box helicase (NCU06365), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_957306.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCTTCCA GCCACCATAG CGCCAATGCT ACTACTACTG ATCCACTGAA AGGGCTCAAG 
    CGCAAACGCC TGTCCACCGA ACCCGAAGCG TCGACGGAGC CGAGGCACGA TGCCGCTTTC
    GCCGGAAATC CTAGCTGCGA CTCCCAGGAG AGTTCCGGTA GTTGTGGCCC AGCAACAACG
    GGGCCAAAGA AGCGGCGAGG TCGTCCCCCC AAGCTTGTTT CCGAGCCTAC CATTGCCGAA
    AGCGATATCG AATGGCCGGC GTGGTTCAAG GAACTCGAGA AGACGCACAG GGCTTTGAAC
    CTCGTCTACA CCTTTTGCTC TACCCGAAAG CATCTGGCGA CTACGTTTGA GACGATACGG
    TCCACGGTTG AGTCACACAC AAAGCGTTCA TTAACGATTG AGGATGTAGC TGCTATTGTA
    GCCTTGCGTC CGGAGAGCAT TCAGTTTGCC TATGTCGATG AGCTAATGCT ACAGCTGGAT
    GCGAGGGGTG CCGAAAAGGA TGAGGCCTTC AAGACAGGAA GGTATGCTGG GAAGTCATCT
    CAGTCCTTTG TACCCGACGC GTCTGTTGGC GGTATGACCG GCTTGGAAGG ACTAGGTGAT
    GCTGGCCCGG ACCATACCGG TGAAGATGAG CAAGAGTCGA AATCTGGTCG GGAAGTCCTC
    TACTTTGAGT TCATTGATGA AGATCTCAAG CGGCAAGTCC AGGACAAGAA AACAGGAGTC
    CCGACTAAGC CGACTCGCAG GCTTCGACAT GAGCATGTCA AAATGCCTGT GTATAGTCAG
    AAGCAAATGA CGGCACTAAT AGAAAAGAGG AACACCAAGT TCGCCAATGC AGTCAACATT
    TTCTTGAACC GCTGTGCTGC AGAAAGTCTA GATCCGGAAA TGGCACTCAG AAGCGAGTCC
    GAACCCTTCG TTCCCAGGCC CTCAACACCC AGAGCGTCAA CCCCCCAGAC AAAACCCAGC
    ACTTTGCCCG CAAGCATCCC GAAGGAGAGG AAAGGTATTC CGGAGATAGT ACAGGAGCTG
    AAGGATAGTC CCTGGTACAC GGGTCAGATA GTCCCCGATG GCCATCGCGT GAACGAAGCT
    CAGGAACCTG TTTACGGTTA TCTGAATTTT CTACTCAGCC AAGATCTGGT TAATGCGCTT
    TACAACACCA AGGGAATTAC CCAGTTTTAT GCACATCAAG CTGATGCCAT TAACGCTCTA
    CACGACGGCC GTCACGTGGT AGTGTCCACG TCCACCAGCT CGGGAAAGTC CCTCATCTAT
    CAATTGCCTG TCATACATGC CCTTGAGCAG GACCGGAATA CGCGAGCCAT GTACATCTTC
    CCAACTAAGG CGCTTGCGCA AGACCAGAAG CGGAGCCTGA AGGAAGTCAT GAGCCTTATG
    CCTGGACTGG AAGAGATGAT CGTTGAAACA TTTGACGGCG ATACGCCAAT GCATGACAGA
    AACATCATCC GCGATGATGC ACGCATCATC TTCACGAATC CTGATATGCT GCATATCACG
    ATCTTACCCC AGGAGGAAAG GTGGAGGTCG TTTCTGCAAA ACCTGAAATA TGTTGTTGTG
    GATGAATTGC ATTATTACAA CGGCCTAATG GGTTCACATG TTGCCTTCAT CATGAGACGA
    CTGCGCCGAA TCCTCGCAGC GTTGGGAAAT ACGCATGTAC TCTTCATCTC GTGTTCAGCA
    ACCGTGGCCA ACCCAAAGGA GCATTTCACT ACCATCTTCG GCGTTGAAGA CGTCAAACTC
    ATTGACTTCA ACGGCTCTCC GTCGGGTCGC AAGGAGTTCC TCTGCTGGAA CACTCCGTAC
    AAAGACCCTG GAGATCCGTC CAGCGGACGT GGCAATGCCA AGTTGGAGTG TTCCCGCCTG
    TTCTGCGAGC TCATCCTCCG CGGCGTTCGT GTCATCGCAT TCTGTCGCGT ACGCGAACAC
    TGCGAAAAAC TGGTCAATGC AATCAAACAA GAACTGGAGC ACAGAGGCCG CTCCGAGTGC
    ATGTCTCGAG TCATGGGGTA CCGAGGCGGT TACACTGCCC AGGACAGGCG CCGTATAGAA
    ACTGAAATGT TTGAGGGCAA GCTCATGGGC ATCGTGGCGA CCACCGCTCT TGAAATTGGC
    ATTGACATCG GAAATCTAGA TTGTGTCATA ACTTGGGGGT TTCCGTACAC AATAGCGAAC
    CTCAGACAGC AGAGTGGAAG AGCTGGCCGG CGAAATAGAG ACTCTCTGTC GATTCTTCTA
    GGAGACAGTT TCGCAACCGA CCAGTATTAC ATGCAGAATC CGGATGAACT ATTCACAAAA
    CCGAACTGTG AGCTTAGTAT AGATATGGAT AACATGTTGG TCAAGGAGGG CCACATTCAA
    TGCGCGGCAC ACGAAATGCC AATTCTACCG GCTAAGGATT CCAAATACTT TGGTGACGAT
    CTAGCAACGG TTTGCGAAGA GCGGCTGCTC AAGGGTGATC GGGGCTTTTA CCATTGTCAT
    GATAAGTTTC GACCCAGGCC GTCACAGTTT GTATCCATAC GCGATATCGA AGAAGACCAT
    TTCGCCATCG TCGACGTCAC GAACAACCGG AACGTTGTCC TAGAGGAGTT GGAGGCATCC
    CGAGCGACCT TCACCATCTA CGACGGAGCC ATATTCCTAC ACCAAGGTAA CACCTACCTC
    GTACGGGACT TCAACCCCGA CAATAAGATG GCGCGAGTCG AGAAGGTCAA GGTGGACTGG
    ACCACTCAGC AGCGGGATTA CACGGACATT GACCCGGTGG AGACAGAAGC CATCCGGCGC
    ATCAAAGGAT CGAAATCCCT CGCCTATCAT GGGGTCATCA GAATCACCCA GGTTGTCTTT
    GGCTACTTCA AAGTCGACAA AAAGAATAGA ATACTTGACG CCATCCAAGT TGACAACCCG
    CCCGTTATCC GCTACAGTAA GGGCACCTGG CTCGACGTTC CGAAATCTTC ACTCGCAATC
    CTTACGTCGC GGCGCCTTCA CGTTGGCGGA GCCATCCACG CCGCCCAGCA CGCCATCATG
    AGCCTCATGC CCAACTTCGT CATGAGCATG CCGGGCGACG TGCGCACCGA GTGCAAGAAC
    CACCTCAAAG AGTTTGCCAA GCGGGAGACG CAGCGGAAAC GGCCGGCTCG CCTGACCTTC
    TACGACGCAA AGGGGGGCGC AGGCGGGTCG GGCATCAACA TCAAGGCGTT CGAGTTTATC
    GACACCTTAC TGAGGAAGGC GCTCGGTCGG GTGTTGGCAT GTCAGTGCCG CGAGGGTTGC
    ATCGAGTGCG TGTGCTCAGA GCTCTGCAAG GAGGCTAATT CGGTTATCAG CAAGGCGGGA
    GCTGAGGTGA TACTCAAGAG CTTGTTGGAT GAGGAGATTG ATGTGGAGAG CTTGCCAATG
    GGGCCGGAGG AGATCAGTCC GGCGGGCGTG GAAACGGTGA TTTTGGCGAA ACCGGTGCCG
    CCGCGGAACA GGAAGACGGT CAAGATTATT GGGCTTGCGG GGGAGGAGAT TGAGATTCAG
    GTCAATGATG AGCAGGATGA TGGGGAAGAG GAGGTAGATG GTTCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The genome sequence of the filamentous fungus Neurospora crassa.
    Nature422(6934)859-68(2003 Apr)
    Galagan JE,Calvo SE,Borkovich KA,Selker EU,Read ND,Jaffe D,FitzHugh W,Ma LJ,Smirnov S,Purcell S,Rehman B,Elkins T,Engels R,Wang S,Nielsen CB,Butler J,Endrizzi M,Qui D,Ianakiev P,Bell-Pedersen D,Nelson MA,Werner-Washburne M,Selitrennikoff CP,Kinsey JA,Braun EL,Zelter A,Schulte U,Kothe GO,Jedd G,Mewes W,Staben C,Marcotte E,Greenberg D,Roy A,Foley K,Naylor J,Stange-Thomann N,Barrett R,Gnerre S,Kamal M,Kamvysselis M,Mauceli E,Bielke C,Rudd S,Frishman D,Krystofova S,Rasmussen C,Metzenberg RL,Perkins DD,Kroken S,Cogoni C,Macino G,Catcheside D,Li W,Pratt RJ,Osmani SA,DeSouza CP,Glass L,Orbach MJ,Berglund JA,Voelker R,Yarden O,Plamann M,Seiler S,Dunlap J,Radford A,Aramayo R,Natvig DO,Alex LA,Mannhaupt G,Ebbole DJ,Freitag M,Paulsen I,Sachs MS,Lander ES,Nusbaum C,Birren B