NCU05620 cDNA ORF clone, Neurospora crassa OR74A

Gene
Clones
gRNAs

Summary

Gene Symbol	NCU05620
Entrez Gene ID	3876263
Full Name	proteasome activator subunit 4
Gene Type	protein-coding
Organism	Neurospora crassa OR74A

mRNA and Protein(s)

mRNA	Protein	Name
XM_011396673.1	XP_011394975.1	proteasome activator subunit 4
XM_011396674.1	XP_011394976.1	proteasome activator subunit 4, variant

Pathways from BioSystems

Homologies

Eremothecium gossypii	AGOS_AGL022W	NP_986644.2
Magnaporthe oryzae (rice blast fungus)	MGG_04342	XP_003719463.1
Saccharomyces cerevisiae (baker's yeast)	BLM10	NP_116648.2
Kluyveromyces lactis	KLLA0E12057g	XP_454493.1
Neurospora crassa	NCU05620	XP_960116.2

Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Galagan JE, Calvo SE, Borkovich KA, Selker EU, Read ND, Jaffe D, FitzHugh W, Ma LJ, Smirnov S, Purcell S, Rehman B, Elkins T, Engels R, Wang S, Nielsen CB, Butler J, Endrizzi M, Qui D, Ianakiev P, Bell-Pedersen D, Nelson MA, Werner-Washburne M, Selitrennikoff CP, Kinsey JA, Braun EL, Zelter A, Schulte U, Kothe GO, Jedd G, Mewes W, Staben C, Marcotte E, Greenberg D, Roy A, Foley K, Naylor J, Stange-Thomann N, Barrett R, Gnerre S, Kamal M, Kamvysselis M, Mauceli E, Bielke C, Rudd S, Frishman D, Krystofova S, Rasmussen C, Metzenberg RL, Perkins DD, Kroken S, Cogoni C, Macino G, Catcheside D, Li W, Pratt RJ, Osmani SA, DeSouza CP, Glass L, Orbach MJ, Berglund JA, Voelker R, Yarden O, Plamann M, Seiler S, Dunlap J, Radford A, Aramayo R, Natvig DO, Alex LA, Mannhaupt G, Ebbole DJ, Freitag M, Paulsen I, Sachs MS, Lander ES, Nusbaum C, Birren B
Nature422(6934)859-68(2003 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following NCU05620 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NCU05620 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ONf07834	XM_011396673.1 Latest version!	Neurospora crassa OR74A proteasome activator subunit 4 (NCU05620), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ONf07834	XM_011396674.1 Latest version!	Neurospora crassa OR74A proteasome activator subunit 4, variant (NCU05620), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NCU05620 ( XM_011396673.1 ) cDNA ORF clone, Neurospora crassa OR74A -> XP_011394975.1 Neurospora crassa OR74A proteasome activator subunit 4 (NCU05620), mRNA.

Price & Availability↑

CloneID	ONf07834
Clone ID Related Accession (Same CDS sequence)	XM_011396673.1 , XM_011396674.1
Accession Version	XM_011396673.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5868bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-10
Organism	Neurospora crassa OR74A
Product	proteasome activator subunit 4
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026506).

ATGGACGAGT CTCTAGAGGC GCATCCCGGC GCCGCTGGTC ACTTCGCTGC CGAAGGAGCG 
CATTCCGCCT TCCTCGGCGG TTTGTTAGCC TCTGCCGACA CCATCTCCCG CGCCACATCG 
CCCGGACTGC CATGTTCCAA CGGCTCAGAC CAAGATGACC AGAAGCGCTA CCGCCCCCGA 
ACCTTCAGTT ACTTCCGCCT GCTTCCCTTC GAAGTCGAGG AGGAGTCGCA CCGGGATGCC 
GCTTTGAACG GCATCCTCAA GAACCTCTAC GTCTCTATAA TGGCCGAAGA CTTTTCCCCG 
GGCGCTGTAC ACTGGACTCG CGAGCTTCAG GGTTGGCTAA ACCTCAAGTT TGAGATGTCG 
AGGGAACTTC GGGCCCGTCT CGCGCATCTC TACTTCCATT TGGCGCTTGC CCCCGGGCTT 
GATCCCAACA CAGCCGATAG GTTCGCGAGA ATGGTAGTTA CCTTGACGCG TAAGCGACAT 
TATCTGAAGC CAGGCCAAGA CCTGACGCTG GACTGGCGAC CACTCTGGAA AGAGCTCAAG 
GGCTTGGTTT TCCCTTCGGA AACGGCCCCT CATCAAGGTA GCAGAAAGCG TGCTCAAAAG 
CACATTATGA AGCTCTGTCT TCACGGGAGC GTCTACTTCG ATCCCAAGGA GCGCCGTGCG 
ATACTGGATG AGCTGCTGCC ATACTTCAGC ACCTCCGACA TGTCCAATGC CTACATTGTT 
GCCGGCGCTA TCAATACATT GCTTCCAACC GGCCCAGCAC CCCCAACTGA GCCTCAGTCA 
CAGCCGTCTG ACTTCGTGCC CACCTTCTTC CACTTGTGGT CTCTTATGAC GCGGTCCAAG 
GCCTTTGATG TGGGCTTTAT TGACATCTTT TCCCGAATGG CCAGGGATTA TCTAAGCTGT 
ACACATGTCC CATTCACTGA GCACGGTATC TTTACTCGTG AACAATCCGA CCTCATCTTT 
ACAGCCATTC TACGCCTGAC GGAGATCCCT GTCGGACAAG CCAACTCCCC ATATGCCTCG 
CTTGACTACT CTGCTGGGCT GGGGATTTAT CTGGAAAAGG ACAAGAAAAA GTATCCAGTG 
CCCTACATGG TTGCTAGGTG GATTGTACAC TCGATATCAC CACTTTGCCT TGACCAGGAA 
AGTTCCGTCA TGTCCAACCT TGAGGGTCTA ATGGAGTCAA TAGACACTTT CTTCCATCCT 
TCGAACCAGG GATCGTGGTC TATGTTTCTC GCCCAATTGA CTTTCTTCTT GACCGATATT 
TTCGTTTCAC GCTGGAATCG TGAGCAGAGC GGTGAGCTCG ATATTCCCGC AGACCGTCGG 
ATAAACGACG ACCTGAAGAA GCGTTTTGTG CTCTCCCTCA GAGAGGTCAC CTTCATGGGC 
CTCTTCTCCA AGAGCTCAAA GGTCGTCAAC TACTACTACA CTGCCCTGCA AGGACTCGCA 
TACCTCGAGC CAGATCTGAT GCTTCCTGGT GCCCTGCAGC GATTCTACCC GAGCTTGCAA 
GGGCTTGTAG AAGTGCACCG GACGACTTCA AGTCTCTGCA GTCTTCAGAT GATTGCCAAC 
ATCATGTCGA AGCACAAGGG CTACCGCTGC CATATCACCG CTCTACTGGC TTTAGCCCTT 
CCCGGTATTG ATGCCAACGA TCTCAATAAA ACACAGTATA CGTTGAACTT CATCCAAAGT 
GTGGCGTACA GCATCCCTTT TGTCCCGCTT GTCGATGAGA ATAACGACAT TCATGACACG 
AGCCTTGCCA TGAACTGGGT TCAGGGGGAG ATGGAGAGAA TGGAGCGCGA GGGGCAAGAT 
GTCAAGATCA ACTACAACGA GGAGCTTAGT GACGAAGACG AAGCAAACAT CCTTCGATCC 
TCCACCGCTG GTCTTGGAGA GTTTGTCATG GCTTTACTAG GCAAGGTGTT TGCCCTGCTA 
GAAAACCTTC CAGACGCGAG CCATCTACGA ACAGGCTCCC CAGAGGACAA CGTGATCAAC 
ACGCTCCCTG CTGCACTCAC GCCTTTGTTC GCATCGCTGT CTCCTCAGCT CTTTGACTCG 
GCGTTGGAGA AGCTGGCAAA CTTTGTGTCG ACTCATGTAG TGCATCAAGC TCGGGATGCC 
ATGGCGTGGA TTTGCAATGC CTTGTGCAAG GTCAACCCGG AAAAGACGCT CAAGGTCTTC 
ATTCCCATGC TGATTGTCAA CATTCGCAAT GAGATCGACT ACAACGGTGC TGCTTCAGAT 
CGAAGCAGTG GTACCGAAGT GCTTCCCAGG GACAGAGCGT TGGTCTGGCA TGTGAGCATG 
CTCAGCATGT GCGTGGTCCA CGTCGGCAGT GAGGTTCTCA AATACAAGGA CGAGCTTTTC 
GGAATCGCCA AGTACATGCA GGAAAAATGC CGTGGTCTTC CCACCATTCA CATCTCCAAC 
TACGTCCATC ATTTACTGCT CAACCTTACC CACACCTACC CAATCGATAA TGCTCTGTAT 
GAGCCCGATG TAATTGCTCG CGGCATCGAC GTCAGCGACT GGGGCCGTAC GACTGAGCCA 
TCTGAACTCA CCATCAAGTG GCACCGGCCG TCTCCCGCCG AAGTGGCTTT TGCTGTGGAG 
CTGTTTGAGA GCGAGGCTCG TGGTGCCACG GAGAGACTCA TGCAGCTCAT GAGTGACGAT 
CCTCCGGTTA GCAGAAAGGG AAAGAACAAA GAGTGGTCAG ATGAGTTGTC GCGGAGCTTG 
ACAGCGCTTC GTCTTTTGAT CTCGGGCGTC TCAACCCTTT TCGACCCTGT CCGTGCGTCA 
GGAGAAGCAA CTGGCTCCAG CGGTAACCAG GATACCGATG CCGCCGGTGA CACAATTATG 
GAAGAGGACA ATGACCCGCT GGCTGAGATC GCCGACGATG ATGAACTCAA GCGCACATTC 
CGGTATCCCG CTGGATACGT ATTGACCACG GATGATCCTC TCTACCATCG CGTGCACGAT 
CTTCGCGAGG AGGTGGGTAG GACATTATCG CAAACCCATT CCTTCTTGAA CGCAAACCAG 
CAGGATGATG TTGCATGCTT CACGGCCCTA TATAGCGCAT ACAGGACGTG GATCACTGAC 
GTGGGAATTG AACGTTCTGC TCATCCCTTG GAGCGTTTGA CTCGCTTGTA CAAGGCAGAT 
ATTTCCCCCT TCAAGATTTC GGGTCTGCGA AAGGTTTATC CTCGACCACT GTTGATCAAG 
CGTGCGGATG CGTACCAGCT CCAGCGTGTC AAATACAACT CGGCGTATCG CACCAGCAGT 
GAGCTGGACC GGCGCCTGCT CTTGGATCTT GCCGAGTCTT GCACGTCATC ATACGCGGAT 
GTTCGTCGGG TCGCACAAAG TGCTCAAGAC TCGTCTCTTA AGGTCATCAT TGGTGGAAGG 
CCTTTAGTTA TTCCAGTCAT CCTCAATGCT TTGCGTAAGG CACTGGATGA TATGGATCAT 
GACCGTATCA AGGGCGCCAT GTATACCTTG TTCTTTACTA CTCTGCTCAA GACGTGTATG 
AAAGACTGGC GGTTCGCTCC CGAGCTTATG AGGCTCTACA TCGAAACCGC CAGTGTAGAC 
AAGACGTCTA TTCAGAATCT GGGCGCCACG GCTCTCTACC CGCTCATTGA TTTCGGCAAG 
CCGTTTGAGC GCATGATACT CTTTGATCGG TCGGTCGCTG ACACCATTCG ACCCCCGCAG 
GACTTCTCAG CGTCCATCCA ACGGCGTCAT GAGTTCATTA AAGAACGCCG CGCTAAGGTG 
GAGGAGAAGA AGGCTGCATT AGGGTTGGAA CTTATTAATA AGGCCAAGGC TTCGCACTGG 
AAGGTCGCCA CGAGGTGTGC CATGTTTGCA CTCAACATGT CTCTTCGTTT TGAGACACTA 
GCGCCTCCCG AGTTCATCGA ATTGGTCGTT ACTGGCACGA ATGATCCTCA TCCCGGTCTT 
CGCTCGACTT ATCTGGCTGC ATTCAGTGGG CTATGTACCT CCATAGACAT GAGGGCGGTC 
TACCAGCACA GCTATGAGAA GTACTTGCTA GAGCAAGCTG TCGGAGACAA TAAGTTTGAG 
GTTCCCGTTC CTGAGGACGA TCCTGAGTGG ACTCAAAAGT TTCTTGCCCA GTTCGAGAAC 
ACTGATGAGA TCCAGTACTT TGTCGATAGT GACTATCCTG GTTGGTTGGT GTGGGGCAAA 
AAGTTCATAG CCTCATCCGC GAACCCGGAG CCATTCCTAG GATACGACGA GATCGAGACA 
GCTGCTCGTC AGCAAATCGG AAAGCTTTTG ACCCGCGACT GGTTCAAGCA ATGCTTCGAG 
TACCTCAAGC AGGAACCCAG AGATGCCGGC GCAGACAGAT TCAGAATGTC TAACGTCGTA 
CTTCTCATGC AGGTCTTTGA CCTCATGAAC TATGGTCAGA CAGCCGCCAC GTTTGACGAC 
ATCGTTGAAT TGACCAAGGA GATTTACGGA GATGGCGGTG ACAAGCATCA GCATCGTGCA 
ACGGCAGAAA TCGTCGGAGC CATGCTGTCT GGAAGTAGCG ATGATCCTCG CGAGTTCCGC 
GACAAGGTCT GGGCTTTCGC AGCACCCTTG ATGCTCAAGG TGATCGCAGA CGATTTGACG 
CCGGATAATC TGCAGTACTG GATGACATGT CTCCATCTCA CGGTTGATTC CAAGGACCCT 
AGACGTTCGA AGGAGCTCAT CGACCCTCTG GCGAACTTCC GCCTTGATAT GAACTCCAAT 
GCAGCCTTCA AGGAGTCCGC CAAGATCCAA TTGCTCGAGT TCATCATTAA TGATGCTGGC 
TGGCATTTCC GCCGTGACAA GCCCATTCTT GAAGACTTCC TGGCCCATAT CGACCATCCC 
TACAAGTCTG TTCGAGAATC CATTGGTAGA GTCATTGCGA CGATTTATCG GACCAGATAC 
CATGAGTCCT TCCCCAATGT CTGGTCTCTG CTAGAGCAAA ACAAGAGCGC CTCCTCGATT 
GGTATTCGCC CTTACGAGGC AACTGAAGAC TTTACGGCTA CGATCCGCGA GGTCTTTGAT 
CGCATCGAGA AGTGGCGTCA TGAGCGTACT CCCGGACAGC AGACTCCCTC CAGCTATACG 
TCCGGGTCCA AGACGGTACT TGTGTGGCTG GATAGTATGC TCGCCTCGCA GGAATGCATC 
CAACTTGTGC CCTTCTTTCC CGAGCCGTTT ATTGACCAGC TGTTGCACAT GATGGACGTC 
AAGGAAGACC CTGAACTTAT GCGGCTCGCA TACCACGTTT ACCGGCACAT GCCCAACATC 
CCCTTCCGGT CCGGTGAGGA TGACGCATTC ATTGCCGCTC TGATCCGGGT TGGTAAGACA 
GCGGCCTCAT GGCATCAGCG CCTACGCGCT CTGGTCAACA TGCAAGTCAT CTACTTCCGT 
CGTCTCTTCC TAACCCAACC AGCCCAGCGA CAAATGCTCT TCGACGCTGT CGGCGACATG 
TTGTCCGACG TGCAACTCGA AGTCCGCGAC GGGGCCAGCG CTACACTTGC TGGCATGATA 
AGATGCTCGC CGGAGAGAAT TCGTAACCCC ATTATCGAGC AGCTCAAGGC CCGCTTCCAG 
CTGCAACTGG AGATGAACCC GATGCCCAAG CGCAAGTTGC CTGGCACCGA TACGCCCGTG 
GACGTGCAGA AACAGATTAT CAGACGGCAT GCGGCCGTGC TGGGCTTAGG CGCCCTCATC 
GAGGCGTTCC CCTATGCGAC GCCGCCCCCT AAGTGGATGC CCGAGGTATT GGCGTACTTG 
GCGACGCACG CGGCGAGTGA TCCGGGTGTG GTGGGTAAGG CGACTAAGGG GATTTTGGCG 
GAATTCAAGA AGACGAGGCA GGATAGTTGG ACGGTTGATC AGAAGTACTT TACAAGCGAG 
CAACTGGAGG ATCTGGAGGG TGTGTTGTGG AAGAGTTACT TTGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011394975.1
CDS	687..6554
Translation	MDESLEAHPGAAGHFAAEGAHSAFLGGLLASADTISRATSPGLPCSNGSDQDDQKRYRPRTFSYFRLLPFEVEEESHRDAALNGILKNLYVSIMAEDFSPGAVHWTRELQGWLNLKFEMSRELRARLAHLYFHLALAPGLDPNTADRFARMVVTLTRKRHYLKPGQDLTLDWRPLWKELKGLVFPSETAPHQGSRKRAQKHIMKLCLHGSVYFDPKERRAILDELLPYFSTSDMSNAYIVAGAINTLLPTGPAPPTEPQSQPSDFVPTFFHLWSLMTRSKAFDVGFIDIFSRMARDYLSCTHVPFTEHGIFTREQSDLIFTAILRLTEIPVGQANSPYASLDYSAGLGIYLEKDKKKYPVPYMVARWIVHSISPLCLDQESSVMSNLEGLMESIDTFFHPSNQGSWSMFLAQLTFFLTDIFVSRWNREQSGELDIPADRRINDDLKKRFVLSLREVTFMGLFSKSSKVVNYYYTALQGLAYLEPDLMLPGALQRFYPSLQGLVEVHRTTSSLCSLQMIANIMSKHKGYRCHITALLALALPGIDANDLNKTQYTLNFIQSVAYSIPFVPLVDENNDIHDTSLAMNWVQGEMERMEREGQDVKINYNEELSDEDEANILRSSTAGLGEFVMALLGKVFALLENLPDASHLRTGSPEDNVINTLPAALTPLFASLSPQLFDSALEKLANFVSTHVVHQARDAMAWICNALCKVNPEKTLKVFIPMLIVNIRNEIDYNGAASDRSSGTEVLPRDRALVWHVSMLSMCVVHVGSEVLKYKDELFGIAKYMQEKCRGLPTIHISNYVHHLLLNLTHTYPIDNALYEPDVIARGIDVSDWGRTTEPSELTIKWHRPSPAEVAFAVELFESEARGATERLMQLMSDDPPVSRKGKNKEWSDELSRSLTALRLLISGVSTLFDPVRASGEATGSSGNQDTDAAGDTIMEEDNDPLAEIADDDELKRTFRYPAGYVLTTDDPLYHRVHDLREEVGRTLSQTHSFLNANQQDDVACFTALYSAYRTWITDVGIERSAHPLERLTRLYKADISPFKISGLRKVYPRPLLIKRADAYQLQRVKYNSAYRTSSELDRRLLLDLAESCTSSYADVRRVAQSAQDSSLKVIIGGRPLVIPVILNALRKALDDMDHDRIKGAMYTLFFTTLLKTCMKDWRFAPELMRLYIETASVDKTSIQNLGATALYPLIDFGKPFERMILFDRSVADTIRPPQDFSASIQRRHEFIKERRAKVEEKKAALGLELINKAKASHWKVATRCAMFALNMSLRFETLAPPEFIELVVTGTNDPHPGLRSTYLAAFSGLCTSIDMRAVYQHSYEKYLLEQAVGDNKFEVPVPEDDPEWTQKFLAQFENTDEIQYFVDSDYPGWLVWGKKFIASSANPEPFLGYDEIETAARQQIGKLLTRDWFKQCFEYLKQEPRDAGADRFRMSNVVLLMQVFDLMNYGQTAATFDDIVELTKEIYGDGGDKHQHRATAEIVGAMLSGSSDDPREFRDKVWAFAAPLMLKVIADDLTPDNLQYWMTCLHLTVDSKDPRRSKELIDPLANFRLDMNSNAAFKESAKIQLLEFIINDAGWHFRRDKPILEDFLAHIDHPYKSVRESIGRVIATIYRTRYHESFPNVWSLLEQNKSASSIGIRPYEATEDFTATIREVFDRIEKWRHERTPGQQTPSSYTSGSKTVLVWLDSMLASQECIQLVPFFPEPFIDQLLHMMDVKEDPELMRLAYHVYRHMPNIPFRSGEDDAFIAALIRVGKTAASWHQRLRALVNMQVIYFRRLFLTQPAQRQMLFDAVGDMLSDVQLEVRDGASATLAGMIRCSPERIRNPIIEQLKARFQLQLEMNPMPKRKLPGTDTPVDVQKQIIRRHAAVLGLGALIEAFPYATPPPKWMPEVLAYLATHAASDPGVVGKATKGILAEFKKTRQDSWTVDQKYFTSEQLEDLEGVLWKSYFA

Target ORF information:

RefSeq Version	XM_011396673.1
Organism	Neurospora crassa OR74A
Definition	Neurospora crassa OR74A proteasome activator subunit 4 (NCU05620), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011396673.1

ORF Insert Sequence:

ATGGACGAGT CTCTAGAGGC GCATCCCGGC GCCGCTGGTC ACTTCGCTGC CGAAGGAGCG 
CATTCCGCCT TCCTCGGCGG TTTGTTAGCC TCTGCCGACA CCATCTCCCG CGCCACATCG 
CCCGGACTGC CATGTTCCAA CGGCTCAGAC CAAGATGACC AGAAGCGCTA CCGCCCCCGA 
ACCTTCAGTT ACTTCCGCCT GCTTCCCTTC GAAGTCGAGG AGGAGTCGCA CCGGGATGCC 
GCTTTGAACG GCATCCTCAA GAACCTCTAC GTCTCTATAA TGGCCGAAGA CTTTTCCCCG 
GGCGCTGTAC ACTGGACTCG CGAGCTTCAG GGTTGGCTAA ACCTCAAGTT TGAGATGTCG 
AGGGAACTTC GGGCCCGTCT CGCGCATCTC TACTTCCATT TGGCGCTTGC CCCCGGGCTT 
GATCCCAACA CAGCCGATAG GTTCGCGAGA ATGGTAGTTA CCTTGACGCG TAAGCGACAT 
TATCTGAAGC CAGGCCAAGA CCTGACGCTG GACTGGCGAC CACTCTGGAA AGAGCTCAAG 
GGCTTGGTTT TCCCTTCGGA AACGGCCCCT CATCAAGGTA GCAGAAAGCG TGCTCAAAAG 
CACATTATGA AGCTCTGTCT TCACGGGAGC GTCTACTTCG ATCCCAAGGA GCGCCGTGCG 
ATACTGGATG AGCTGCTGCC ATACTTCAGC ACCTCCGACA TGTCCAATGC CTACATTGTT 
GCCGGCGCTA TCAATACATT GCTTCCAACC GGCCCAGCAC CCCCAACTGA GCCTCAGTCA 
CAGCCGTCTG ACTTCGTGCC CACCTTCTTC CACTTGTGGT CTCTTATGAC GCGGTCCAAG 
GCCTTTGATG TGGGCTTTAT TGACATCTTT TCCCGAATGG CCAGGGATTA TCTAAGCTGT 
ACACATGTCC CATTCACTGA GCACGGTATC TTTACTCGTG AACAATCCGA CCTCATCTTT 
ACAGCCATTC TACGCCTGAC GGAGATCCCT GTCGGACAAG CCAACTCCCC ATATGCCTCG 
CTTGACTACT CTGCTGGGCT GGGGATTTAT CTGGAAAAGG ACAAGAAAAA GTATCCAGTG 
CCCTACATGG TTGCTAGGTG GATTGTACAC TCGATATCAC CACTTTGCCT TGACCAGGAA 
AGTTCCGTCA TGTCCAACCT TGAGGGTCTA ATGGAGTCAA TAGACACTTT CTTCCATCCT 
TCGAACCAGG GATCGTGGTC TATGTTTCTC GCCCAATTGA CTTTCTTCTT GACCGATATT 
TTCGTTTCAC GCTGGAATCG TGAGCAGAGC GGTGAGCTCG ATATTCCCGC AGACCGTCGG 
ATAAACGACG ACCTGAAGAA GCGTTTTGTG CTCTCCCTCA GAGAGGTCAC CTTCATGGGC 
CTCTTCTCCA AGAGCTCAAA GGTCGTCAAC TACTACTACA CTGCCCTGCA AGGACTCGCA 
TACCTCGAGC CAGATCTGAT GCTTCCTGGT GCCCTGCAGC GATTCTACCC GAGCTTGCAA 
GGGCTTGTAG AAGTGCACCG GACGACTTCA AGTCTCTGCA GTCTTCAGAT GATTGCCAAC 
ATCATGTCGA AGCACAAGGG CTACCGCTGC CATATCACCG CTCTACTGGC TTTAGCCCTT 
CCCGGTATTG ATGCCAACGA TCTCAATAAA ACACAGTATA CGTTGAACTT CATCCAAAGT 
GTGGCGTACA GCATCCCTTT TGTCCCGCTT GTCGATGAGA ATAACGACAT TCATGACACG 
AGCCTTGCCA TGAACTGGGT TCAGGGGGAG ATGGAGAGAA TGGAGCGCGA GGGGCAAGAT 
GTCAAGATCA ACTACAACGA GGAGCTTAGT GACGAAGACG AAGCAAACAT CCTTCGATCC 
TCCACCGCTG GTCTTGGAGA GTTTGTCATG GCTTTACTAG GCAAGGTGTT TGCCCTGCTA 
GAAAACCTTC CAGACGCGAG CCATCTACGA ACAGGCTCCC CAGAGGACAA CGTGATCAAC 
ACGCTCCCTG CTGCACTCAC GCCTTTGTTC GCATCGCTGT CTCCTCAGCT CTTTGACTCG 
GCGTTGGAGA AGCTGGCAAA CTTTGTGTCG ACTCATGTAG TGCATCAAGC TCGGGATGCC 
ATGGCGTGGA TTTGCAATGC CTTGTGCAAG GTCAACCCGG AAAAGACGCT CAAGGTCTTC 
ATTCCCATGC TGATTGTCAA CATTCGCAAT GAGATCGACT ACAACGGTGC TGCTTCAGAT 
CGAAGCAGTG GTACCGAAGT GCTTCCCAGG GACAGAGCGT TGGTCTGGCA TGTGAGCATG 
CTCAGCATGT GCGTGGTCCA CGTCGGCAGT GAGGTTCTCA AATACAAGGA CGAGCTTTTC 
GGAATCGCCA AGTACATGCA GGAAAAATGC CGTGGTCTTC CCACCATTCA CATCTCCAAC 
TACGTCCATC ATTTACTGCT CAACCTTACC CACACCTACC CAATCGATAA TGCTCTGTAT 
GAGCCCGATG TAATTGCTCG CGGCATCGAC GTCAGCGACT GGGGCCGTAC GACTGAGCCA 
TCTGAACTCA CCATCAAGTG GCACCGGCCG TCTCCCGCCG AAGTGGCTTT TGCTGTGGAG 
CTGTTTGAGA GCGAGGCTCG TGGTGCCACG GAGAGACTCA TGCAGCTCAT GAGTGACGAT 
CCTCCGGTTA GCAGAAAGGG AAAGAACAAA GAGTGGTCAG ATGAGTTGTC GCGGAGCTTG 
ACAGCGCTTC GTCTTTTGAT CTCGGGCGTC TCAACCCTTT TCGACCCTGT CCGTGCGTCA 
GGAGAAGCAA CTGGCTCCAG CGGTAACCAG GATACCGATG CCGCCGGTGA CACAATTATG 
GAAGAGGACA ATGACCCGCT GGCTGAGATC GCCGACGATG ATGAACTCAA GCGCACATTC 
CGGTATCCCG CTGGATACGT ATTGACCACG GATGATCCTC TCTACCATCG CGTGCACGAT 
CTTCGCGAGG AGGTGGGTAG GACATTATCG CAAACCCATT CCTTCTTGAA CGCAAACCAG 
CAGGATGATG TTGCATGCTT CACGGCCCTA TATAGCGCAT ACAGGACGTG GATCACTGAC 
GTGGGAATTG AACGTTCTGC TCATCCCTTG GAGCGTTTGA CTCGCTTGTA CAAGGCAGAT 
ATTTCCCCCT TCAAGATTTC GGGTCTGCGA AAGGTTTATC CTCGACCACT GTTGATCAAG 
CGTGCGGATG CGTACCAGCT CCAGCGTGTC AAATACAACT CGGCGTATCG CACCAGCAGT 
GAGCTGGACC GGCGCCTGCT CTTGGATCTT GCCGAGTCTT GCACGTCATC ATACGCGGAT 
GTTCGTCGGG TCGCACAAAG TGCTCAAGAC TCGTCTCTTA AGGTCATCAT TGGTGGAAGG 
CCTTTAGTTA TTCCAGTCAT CCTCAATGCT TTGCGTAAGG CACTGGATGA TATGGATCAT 
GACCGTATCA AGGGCGCCAT GTATACCTTG TTCTTTACTA CTCTGCTCAA GACGTGTATG 
AAAGACTGGC GGTTCGCTCC CGAGCTTATG AGGCTCTACA TCGAAACCGC CAGTGTAGAC 
AAGACGTCTA TTCAGAATCT GGGCGCCACG GCTCTCTACC CGCTCATTGA TTTCGGCAAG 
CCGTTTGAGC GCATGATACT CTTTGATCGG TCGGTCGCTG ACACCATTCG ACCCCCGCAG 
GACTTCTCAG CGTCCATCCA ACGGCGTCAT GAGTTCATTA AAGAACGCCG CGCTAAGGTG 
GAGGAGAAGA AGGCTGCATT AGGGTTGGAA CTTATTAATA AGGCCAAGGC TTCGCACTGG 
AAGGTCGCCA CGAGGTGTGC CATGTTTGCA CTCAACATGT CTCTTCGTTT TGAGACACTA 
GCGCCTCCCG AGTTCATCGA ATTGGTCGTT ACTGGCACGA ATGATCCTCA TCCCGGTCTT 
CGCTCGACTT ATCTGGCTGC ATTCAGTGGG CTATGTACCT CCATAGACAT GAGGGCGGTC 
TACCAGCACA GCTATGAGAA GTACTTGCTA GAGCAAGCTG TCGGAGACAA TAAGTTTGAG 
GTTCCCGTTC CTGAGGACGA TCCTGAGTGG ACTCAAAAGT TTCTTGCCCA GTTCGAGAAC 
ACTGATGAGA TCCAGTACTT TGTCGATAGT GACTATCCTG GTTGGTTGGT GTGGGGCAAA 
AAGTTCATAG CCTCATCCGC GAACCCGGAG CCATTCCTAG GATACGACGA GATCGAGACA 
GCTGCTCGTC AGCAAATCGG AAAGCTTTTG ACCCGCGACT GGTTCAAGCA ATGCTTCGAG 
TACCTCAAGC AGGAACCCAG AGATGCCGGC GCAGACAGAT TCAGAATGTC TAACGTCGTA 
CTTCTCATGC AGGTCTTTGA CCTCATGAAC TATGGTCAGA CAGCCGCCAC GTTTGACGAC 
ATCGTTGAAT TGACCAAGGA GATTTACGGA GATGGCGGTG ACAAGCATCA GCATCGTGCA 
ACGGCAGAAA TCGTCGGAGC CATGCTGTCT GGAAGTAGCG ATGATCCTCG CGAGTTCCGC 
GACAAGGTCT GGGCTTTCGC AGCACCCTTG ATGCTCAAGG TGATCGCAGA CGATTTGACG 
CCGGATAATC TGCAGTACTG GATGACATGT CTCCATCTCA CGGTTGATTC CAAGGACCCT 
AGACGTTCGA AGGAGCTCAT CGACCCTCTG GCGAACTTCC GCCTTGATAT GAACTCCAAT 
GCAGCCTTCA AGGAGTCCGC CAAGATCCAA TTGCTCGAGT TCATCATTAA TGATGCTGGC 
TGGCATTTCC GCCGTGACAA GCCCATTCTT GAAGACTTCC TGGCCCATAT CGACCATCCC 
TACAAGTCTG TTCGAGAATC CATTGGTAGA GTCATTGCGA CGATTTATCG GACCAGATAC 
CATGAGTCCT TCCCCAATGT CTGGTCTCTG CTAGAGCAAA ACAAGAGCGC CTCCTCGATT 
GGTATTCGCC CTTACGAGGC AACTGAAGAC TTTACGGCTA CGATCCGCGA GGTCTTTGAT 
CGCATCGAGA AGTGGCGTCA TGAGCGTACT CCCGGACAGC AGACTCCCTC CAGCTATACG 
TCCGGGTCCA AGACGGTACT TGTGTGGCTG GATAGTATGC TCGCCTCGCA GGAATGCATC 
CAACTTGTGC CCTTCTTTCC CGAGCCGTTT ATTGACCAGC TGTTGCACAT GATGGACGTC 
AAGGAAGACC CTGAACTTAT GCGGCTCGCA TACCACGTTT ACCGGCACAT GCCCAACATC 
CCCTTCCGGT CCGGTGAGGA TGACGCATTC ATTGCCGCTC TGATCCGGGT TGGTAAGACA 
GCGGCCTCAT GGCATCAGCG CCTACGCGCT CTGGTCAACA TGCAAGTCAT CTACTTCCGT 
CGTCTCTTCC TAACCCAACC AGCCCAGCGA CAAATGCTCT TCGACGCTGT CGGCGACATG 
TTGTCCGACG TGCAACTCGA AGTCCGCGAC GGGGCCAGCG CTACACTTGC TGGCATGATA 
AGATGCTCGC CGGAGAGAAT TCGTAACCCC ATTATCGAGC AGCTCAAGGC CCGCTTCCAG 
CTGCAACTGG AGATGAACCC GATGCCCAAG CGCAAGTTGC CTGGCACCGA TACGCCCGTG 
GACGTGCAGA AACAGATTAT CAGACGGCAT GCGGCCGTGC TGGGCTTAGG CGCCCTCATC 
GAGGCGTTCC CCTATGCGAC GCCGCCCCCT AAGTGGATGC CCGAGGTATT GGCGTACTTG 
GCGACGCACG CGGCGAGTGA TCCGGGTGTG GTGGGTAAGG CGACTAAGGG GATTTTGGCG 
GAATTCAAGA AGACGAGGCA GGATAGTTGG ACGGTTGATC AGAAGTACTT TACAAGCGAG 
CAACTGGAGG ATCTGGAGGG TGTGTTGTGG AAGAGTTACT TTGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NCU05620 ( XM_011396674.1 ) cDNA ORF clone, Neurospora crassa OR74A -> XP_011394976.1 Neurospora crassa OR74A proteasome activator subunit 4, variant (NCU05620), mRNA.

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CloneID	ONf07834
Clone ID Related Accession (Same CDS sequence)	XM_011396673.1 , XM_011396674.1
Accession Version	XM_011396674.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5868bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-10
Organism	Neurospora crassa OR74A
Product	proteasome activator subunit 4, variant
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026506).

ATGGACGAGT CTCTAGAGGC GCATCCCGGC GCCGCTGGTC ACTTCGCTGC CGAAGGAGCG 
CATTCCGCCT TCCTCGGCGG TTTGTTAGCC TCTGCCGACA CCATCTCCCG CGCCACATCG 
CCCGGACTGC CATGTTCCAA CGGCTCAGAC CAAGATGACC AGAAGCGCTA CCGCCCCCGA 
ACCTTCAGTT ACTTCCGCCT GCTTCCCTTC GAAGTCGAGG AGGAGTCGCA CCGGGATGCC 
GCTTTGAACG GCATCCTCAA GAACCTCTAC GTCTCTATAA TGGCCGAAGA CTTTTCCCCG 
GGCGCTGTAC ACTGGACTCG CGAGCTTCAG GGTTGGCTAA ACCTCAAGTT TGAGATGTCG 
AGGGAACTTC GGGCCCGTCT CGCGCATCTC TACTTCCATT TGGCGCTTGC CCCCGGGCTT 
GATCCCAACA CAGCCGATAG GTTCGCGAGA ATGGTAGTTA CCTTGACGCG TAAGCGACAT 
TATCTGAAGC CAGGCCAAGA CCTGACGCTG GACTGGCGAC CACTCTGGAA AGAGCTCAAG 
GGCTTGGTTT TCCCTTCGGA AACGGCCCCT CATCAAGGTA GCAGAAAGCG TGCTCAAAAG 
CACATTATGA AGCTCTGTCT TCACGGGAGC GTCTACTTCG ATCCCAAGGA GCGCCGTGCG 
ATACTGGATG AGCTGCTGCC ATACTTCAGC ACCTCCGACA TGTCCAATGC CTACATTGTT 
GCCGGCGCTA TCAATACATT GCTTCCAACC GGCCCAGCAC CCCCAACTGA GCCTCAGTCA 
CAGCCGTCTG ACTTCGTGCC CACCTTCTTC CACTTGTGGT CTCTTATGAC GCGGTCCAAG 
GCCTTTGATG TGGGCTTTAT TGACATCTTT TCCCGAATGG CCAGGGATTA TCTAAGCTGT 
ACACATGTCC CATTCACTGA GCACGGTATC TTTACTCGTG AACAATCCGA CCTCATCTTT 
ACAGCCATTC TACGCCTGAC GGAGATCCCT GTCGGACAAG CCAACTCCCC ATATGCCTCG 
CTTGACTACT CTGCTGGGCT GGGGATTTAT CTGGAAAAGG ACAAGAAAAA GTATCCAGTG 
CCCTACATGG TTGCTAGGTG GATTGTACAC TCGATATCAC CACTTTGCCT TGACCAGGAA 
AGTTCCGTCA TGTCCAACCT TGAGGGTCTA ATGGAGTCAA TAGACACTTT CTTCCATCCT 
TCGAACCAGG GATCGTGGTC TATGTTTCTC GCCCAATTGA CTTTCTTCTT GACCGATATT 
TTCGTTTCAC GCTGGAATCG TGAGCAGAGC GGTGAGCTCG ATATTCCCGC AGACCGTCGG 
ATAAACGACG ACCTGAAGAA GCGTTTTGTG CTCTCCCTCA GAGAGGTCAC CTTCATGGGC 
CTCTTCTCCA AGAGCTCAAA GGTCGTCAAC TACTACTACA CTGCCCTGCA AGGACTCGCA 
TACCTCGAGC CAGATCTGAT GCTTCCTGGT GCCCTGCAGC GATTCTACCC GAGCTTGCAA 
GGGCTTGTAG AAGTGCACCG GACGACTTCA AGTCTCTGCA GTCTTCAGAT GATTGCCAAC 
ATCATGTCGA AGCACAAGGG CTACCGCTGC CATATCACCG CTCTACTGGC TTTAGCCCTT 
CCCGGTATTG ATGCCAACGA TCTCAATAAA ACACAGTATA CGTTGAACTT CATCCAAAGT 
GTGGCGTACA GCATCCCTTT TGTCCCGCTT GTCGATGAGA ATAACGACAT TCATGACACG 
AGCCTTGCCA TGAACTGGGT TCAGGGGGAG ATGGAGAGAA TGGAGCGCGA GGGGCAAGAT 
GTCAAGATCA ACTACAACGA GGAGCTTAGT GACGAAGACG AAGCAAACAT CCTTCGATCC 
TCCACCGCTG GTCTTGGAGA GTTTGTCATG GCTTTACTAG GCAAGGTGTT TGCCCTGCTA 
GAAAACCTTC CAGACGCGAG CCATCTACGA ACAGGCTCCC CAGAGGACAA CGTGATCAAC 
ACGCTCCCTG CTGCACTCAC GCCTTTGTTC GCATCGCTGT CTCCTCAGCT CTTTGACTCG 
GCGTTGGAGA AGCTGGCAAA CTTTGTGTCG ACTCATGTAG TGCATCAAGC TCGGGATGCC 
ATGGCGTGGA TTTGCAATGC CTTGTGCAAG GTCAACCCGG AAAAGACGCT CAAGGTCTTC 
ATTCCCATGC TGATTGTCAA CATTCGCAAT GAGATCGACT ACAACGGTGC TGCTTCAGAT 
CGAAGCAGTG GTACCGAAGT GCTTCCCAGG GACAGAGCGT TGGTCTGGCA TGTGAGCATG 
CTCAGCATGT GCGTGGTCCA CGTCGGCAGT GAGGTTCTCA AATACAAGGA CGAGCTTTTC 
GGAATCGCCA AGTACATGCA GGAAAAATGC CGTGGTCTTC CCACCATTCA CATCTCCAAC 
TACGTCCATC ATTTACTGCT CAACCTTACC CACACCTACC CAATCGATAA TGCTCTGTAT 
GAGCCCGATG TAATTGCTCG CGGCATCGAC GTCAGCGACT GGGGCCGTAC GACTGAGCCA 
TCTGAACTCA CCATCAAGTG GCACCGGCCG TCTCCCGCCG AAGTGGCTTT TGCTGTGGAG 
CTGTTTGAGA GCGAGGCTCG TGGTGCCACG GAGAGACTCA TGCAGCTCAT GAGTGACGAT 
CCTCCGGTTA GCAGAAAGGG AAAGAACAAA GAGTGGTCAG ATGAGTTGTC GCGGAGCTTG 
ACAGCGCTTC GTCTTTTGAT CTCGGGCGTC TCAACCCTTT TCGACCCTGT CCGTGCGTCA 
GGAGAAGCAA CTGGCTCCAG CGGTAACCAG GATACCGATG CCGCCGGTGA CACAATTATG 
GAAGAGGACA ATGACCCGCT GGCTGAGATC GCCGACGATG ATGAACTCAA GCGCACATTC 
CGGTATCCCG CTGGATACGT ATTGACCACG GATGATCCTC TCTACCATCG CGTGCACGAT 
CTTCGCGAGG AGGTGGGTAG GACATTATCG CAAACCCATT CCTTCTTGAA CGCAAACCAG 
CAGGATGATG TTGCATGCTT CACGGCCCTA TATAGCGCAT ACAGGACGTG GATCACTGAC 
GTGGGAATTG AACGTTCTGC TCATCCCTTG GAGCGTTTGA CTCGCTTGTA CAAGGCAGAT 
ATTTCCCCCT TCAAGATTTC GGGTCTGCGA AAGGTTTATC CTCGACCACT GTTGATCAAG 
CGTGCGGATG CGTACCAGCT CCAGCGTGTC AAATACAACT CGGCGTATCG CACCAGCAGT 
GAGCTGGACC GGCGCCTGCT CTTGGATCTT GCCGAGTCTT GCACGTCATC ATACGCGGAT 
GTTCGTCGGG TCGCACAAAG TGCTCAAGAC TCGTCTCTTA AGGTCATCAT TGGTGGAAGG 
CCTTTAGTTA TTCCAGTCAT CCTCAATGCT TTGCGTAAGG CACTGGATGA TATGGATCAT 
GACCGTATCA AGGGCGCCAT GTATACCTTG TTCTTTACTA CTCTGCTCAA GACGTGTATG 
AAAGACTGGC GGTTCGCTCC CGAGCTTATG AGGCTCTACA TCGAAACCGC CAGTGTAGAC 
AAGACGTCTA TTCAGAATCT GGGCGCCACG GCTCTCTACC CGCTCATTGA TTTCGGCAAG 
CCGTTTGAGC GCATGATACT CTTTGATCGG TCGGTCGCTG ACACCATTCG ACCCCCGCAG 
GACTTCTCAG CGTCCATCCA ACGGCGTCAT GAGTTCATTA AAGAACGCCG CGCTAAGGTG 
GAGGAGAAGA AGGCTGCATT AGGGTTGGAA CTTATTAATA AGGCCAAGGC TTCGCACTGG 
AAGGTCGCCA CGAGGTGTGC CATGTTTGCA CTCAACATGT CTCTTCGTTT TGAGACACTA 
GCGCCTCCCG AGTTCATCGA ATTGGTCGTT ACTGGCACGA ATGATCCTCA TCCCGGTCTT 
CGCTCGACTT ATCTGGCTGC ATTCAGTGGG CTATGTACCT CCATAGACAT GAGGGCGGTC 
TACCAGCACA GCTATGAGAA GTACTTGCTA GAGCAAGCTG TCGGAGACAA TAAGTTTGAG 
GTTCCCGTTC CTGAGGACGA TCCTGAGTGG ACTCAAAAGT TTCTTGCCCA GTTCGAGAAC 
ACTGATGAGA TCCAGTACTT TGTCGATAGT GACTATCCTG GTTGGTTGGT GTGGGGCAAA 
AAGTTCATAG CCTCATCCGC GAACCCGGAG CCATTCCTAG GATACGACGA GATCGAGACA 
GCTGCTCGTC AGCAAATCGG AAAGCTTTTG ACCCGCGACT GGTTCAAGCA ATGCTTCGAG 
TACCTCAAGC AGGAACCCAG AGATGCCGGC GCAGACAGAT TCAGAATGTC TAACGTCGTA 
CTTCTCATGC AGGTCTTTGA CCTCATGAAC TATGGTCAGA CAGCCGCCAC GTTTGACGAC 
ATCGTTGAAT TGACCAAGGA GATTTACGGA GATGGCGGTG ACAAGCATCA GCATCGTGCA 
ACGGCAGAAA TCGTCGGAGC CATGCTGTCT GGAAGTAGCG ATGATCCTCG CGAGTTCCGC 
GACAAGGTCT GGGCTTTCGC AGCACCCTTG ATGCTCAAGG TGATCGCAGA CGATTTGACG 
CCGGATAATC TGCAGTACTG GATGACATGT CTCCATCTCA CGGTTGATTC CAAGGACCCT 
AGACGTTCGA AGGAGCTCAT CGACCCTCTG GCGAACTTCC GCCTTGATAT GAACTCCAAT 
GCAGCCTTCA AGGAGTCCGC CAAGATCCAA TTGCTCGAGT TCATCATTAA TGATGCTGGC 
TGGCATTTCC GCCGTGACAA GCCCATTCTT GAAGACTTCC TGGCCCATAT CGACCATCCC 
TACAAGTCTG TTCGAGAATC CATTGGTAGA GTCATTGCGA CGATTTATCG GACCAGATAC 
CATGAGTCCT TCCCCAATGT CTGGTCTCTG CTAGAGCAAA ACAAGAGCGC CTCCTCGATT 
GGTATTCGCC CTTACGAGGC AACTGAAGAC TTTACGGCTA CGATCCGCGA GGTCTTTGAT 
CGCATCGAGA AGTGGCGTCA TGAGCGTACT CCCGGACAGC AGACTCCCTC CAGCTATACG 
TCCGGGTCCA AGACGGTACT TGTGTGGCTG GATAGTATGC TCGCCTCGCA GGAATGCATC 
CAACTTGTGC CCTTCTTTCC CGAGCCGTTT ATTGACCAGC TGTTGCACAT GATGGACGTC 
AAGGAAGACC CTGAACTTAT GCGGCTCGCA TACCACGTTT ACCGGCACAT GCCCAACATC 
CCCTTCCGGT CCGGTGAGGA TGACGCATTC ATTGCCGCTC TGATCCGGGT TGGTAAGACA 
GCGGCCTCAT GGCATCAGCG CCTACGCGCT CTGGTCAACA TGCAAGTCAT CTACTTCCGT 
CGTCTCTTCC TAACCCAACC AGCCCAGCGA CAAATGCTCT TCGACGCTGT CGGCGACATG 
TTGTCCGACG TGCAACTCGA AGTCCGCGAC GGGGCCAGCG CTACACTTGC TGGCATGATA 
AGATGCTCGC CGGAGAGAAT TCGTAACCCC ATTATCGAGC AGCTCAAGGC CCGCTTCCAG 
CTGCAACTGG AGATGAACCC GATGCCCAAG CGCAAGTTGC CTGGCACCGA TACGCCCGTG 
GACGTGCAGA AACAGATTAT CAGACGGCAT GCGGCCGTGC TGGGCTTAGG CGCCCTCATC 
GAGGCGTTCC CCTATGCGAC GCCGCCCCCT AAGTGGATGC CCGAGGTATT GGCGTACTTG 
GCGACGCACG CGGCGAGTGA TCCGGGTGTG GTGGGTAAGG CGACTAAGGG GATTTTGGCG 
GAATTCAAGA AGACGAGGCA GGATAGTTGG ACGGTTGATC AGAAGTACTT TACAAGCGAG 
CAACTGGAGG ATCTGGAGGG TGTGTTGTGG AAGAGTTACT TTGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011394976.1
CDS	543..6410
Translation	MDESLEAHPGAAGHFAAEGAHSAFLGGLLASADTISRATSPGLPCSNGSDQDDQKRYRPRTFSYFRLLPFEVEEESHRDAALNGILKNLYVSIMAEDFSPGAVHWTRELQGWLNLKFEMSRELRARLAHLYFHLALAPGLDPNTADRFARMVVTLTRKRHYLKPGQDLTLDWRPLWKELKGLVFPSETAPHQGSRKRAQKHIMKLCLHGSVYFDPKERRAILDELLPYFSTSDMSNAYIVAGAINTLLPTGPAPPTEPQSQPSDFVPTFFHLWSLMTRSKAFDVGFIDIFSRMARDYLSCTHVPFTEHGIFTREQSDLIFTAILRLTEIPVGQANSPYASLDYSAGLGIYLEKDKKKYPVPYMVARWIVHSISPLCLDQESSVMSNLEGLMESIDTFFHPSNQGSWSMFLAQLTFFLTDIFVSRWNREQSGELDIPADRRINDDLKKRFVLSLREVTFMGLFSKSSKVVNYYYTALQGLAYLEPDLMLPGALQRFYPSLQGLVEVHRTTSSLCSLQMIANIMSKHKGYRCHITALLALALPGIDANDLNKTQYTLNFIQSVAYSIPFVPLVDENNDIHDTSLAMNWVQGEMERMEREGQDVKINYNEELSDEDEANILRSSTAGLGEFVMALLGKVFALLENLPDASHLRTGSPEDNVINTLPAALTPLFASLSPQLFDSALEKLANFVSTHVVHQARDAMAWICNALCKVNPEKTLKVFIPMLIVNIRNEIDYNGAASDRSSGTEVLPRDRALVWHVSMLSMCVVHVGSEVLKYKDELFGIAKYMQEKCRGLPTIHISNYVHHLLLNLTHTYPIDNALYEPDVIARGIDVSDWGRTTEPSELTIKWHRPSPAEVAFAVELFESEARGATERLMQLMSDDPPVSRKGKNKEWSDELSRSLTALRLLISGVSTLFDPVRASGEATGSSGNQDTDAAGDTIMEEDNDPLAEIADDDELKRTFRYPAGYVLTTDDPLYHRVHDLREEVGRTLSQTHSFLNANQQDDVACFTALYSAYRTWITDVGIERSAHPLERLTRLYKADISPFKISGLRKVYPRPLLIKRADAYQLQRVKYNSAYRTSSELDRRLLLDLAESCTSSYADVRRVAQSAQDSSLKVIIGGRPLVIPVILNALRKALDDMDHDRIKGAMYTLFFTTLLKTCMKDWRFAPELMRLYIETASVDKTSIQNLGATALYPLIDFGKPFERMILFDRSVADTIRPPQDFSASIQRRHEFIKERRAKVEEKKAALGLELINKAKASHWKVATRCAMFALNMSLRFETLAPPEFIELVVTGTNDPHPGLRSTYLAAFSGLCTSIDMRAVYQHSYEKYLLEQAVGDNKFEVPVPEDDPEWTQKFLAQFENTDEIQYFVDSDYPGWLVWGKKFIASSANPEPFLGYDEIETAARQQIGKLLTRDWFKQCFEYLKQEPRDAGADRFRMSNVVLLMQVFDLMNYGQTAATFDDIVELTKEIYGDGGDKHQHRATAEIVGAMLSGSSDDPREFRDKVWAFAAPLMLKVIADDLTPDNLQYWMTCLHLTVDSKDPRRSKELIDPLANFRLDMNSNAAFKESAKIQLLEFIINDAGWHFRRDKPILEDFLAHIDHPYKSVRESIGRVIATIYRTRYHESFPNVWSLLEQNKSASSIGIRPYEATEDFTATIREVFDRIEKWRHERTPGQQTPSSYTSGSKTVLVWLDSMLASQECIQLVPFFPEPFIDQLLHMMDVKEDPELMRLAYHVYRHMPNIPFRSGEDDAFIAALIRVGKTAASWHQRLRALVNMQVIYFRRLFLTQPAQRQMLFDAVGDMLSDVQLEVRDGASATLAGMIRCSPERIRNPIIEQLKARFQLQLEMNPMPKRKLPGTDTPVDVQKQIIRRHAAVLGLGALIEAFPYATPPPKWMPEVLAYLATHAASDPGVVGKATKGILAEFKKTRQDSWTVDQKYFTSEQLEDLEGVLWKSYFA

Target ORF information:

RefSeq Version	XM_011396674.1
Organism	Neurospora crassa OR74A
Definition	Neurospora crassa OR74A proteasome activator subunit 4, variant (NCU05620), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011396674.1

ORF Insert Sequence:

ATGGACGAGT CTCTAGAGGC GCATCCCGGC GCCGCTGGTC ACTTCGCTGC CGAAGGAGCG 
CATTCCGCCT TCCTCGGCGG TTTGTTAGCC TCTGCCGACA CCATCTCCCG CGCCACATCG 
CCCGGACTGC CATGTTCCAA CGGCTCAGAC CAAGATGACC AGAAGCGCTA CCGCCCCCGA 
ACCTTCAGTT ACTTCCGCCT GCTTCCCTTC GAAGTCGAGG AGGAGTCGCA CCGGGATGCC 
GCTTTGAACG GCATCCTCAA GAACCTCTAC GTCTCTATAA TGGCCGAAGA CTTTTCCCCG 
GGCGCTGTAC ACTGGACTCG CGAGCTTCAG GGTTGGCTAA ACCTCAAGTT TGAGATGTCG 
AGGGAACTTC GGGCCCGTCT CGCGCATCTC TACTTCCATT TGGCGCTTGC CCCCGGGCTT 
GATCCCAACA CAGCCGATAG GTTCGCGAGA ATGGTAGTTA CCTTGACGCG TAAGCGACAT 
TATCTGAAGC CAGGCCAAGA CCTGACGCTG GACTGGCGAC CACTCTGGAA AGAGCTCAAG 
GGCTTGGTTT TCCCTTCGGA AACGGCCCCT CATCAAGGTA GCAGAAAGCG TGCTCAAAAG 
CACATTATGA AGCTCTGTCT TCACGGGAGC GTCTACTTCG ATCCCAAGGA GCGCCGTGCG 
ATACTGGATG AGCTGCTGCC ATACTTCAGC ACCTCCGACA TGTCCAATGC CTACATTGTT 
GCCGGCGCTA TCAATACATT GCTTCCAACC GGCCCAGCAC CCCCAACTGA GCCTCAGTCA 
CAGCCGTCTG ACTTCGTGCC CACCTTCTTC CACTTGTGGT CTCTTATGAC GCGGTCCAAG 
GCCTTTGATG TGGGCTTTAT TGACATCTTT TCCCGAATGG CCAGGGATTA TCTAAGCTGT 
ACACATGTCC CATTCACTGA GCACGGTATC TTTACTCGTG AACAATCCGA CCTCATCTTT 
ACAGCCATTC TACGCCTGAC GGAGATCCCT GTCGGACAAG CCAACTCCCC ATATGCCTCG 
CTTGACTACT CTGCTGGGCT GGGGATTTAT CTGGAAAAGG ACAAGAAAAA GTATCCAGTG 
CCCTACATGG TTGCTAGGTG GATTGTACAC TCGATATCAC CACTTTGCCT TGACCAGGAA 
AGTTCCGTCA TGTCCAACCT TGAGGGTCTA ATGGAGTCAA TAGACACTTT CTTCCATCCT 
TCGAACCAGG GATCGTGGTC TATGTTTCTC GCCCAATTGA CTTTCTTCTT GACCGATATT 
TTCGTTTCAC GCTGGAATCG TGAGCAGAGC GGTGAGCTCG ATATTCCCGC AGACCGTCGG 
ATAAACGACG ACCTGAAGAA GCGTTTTGTG CTCTCCCTCA GAGAGGTCAC CTTCATGGGC 
CTCTTCTCCA AGAGCTCAAA GGTCGTCAAC TACTACTACA CTGCCCTGCA AGGACTCGCA 
TACCTCGAGC CAGATCTGAT GCTTCCTGGT GCCCTGCAGC GATTCTACCC GAGCTTGCAA 
GGGCTTGTAG AAGTGCACCG GACGACTTCA AGTCTCTGCA GTCTTCAGAT GATTGCCAAC 
ATCATGTCGA AGCACAAGGG CTACCGCTGC CATATCACCG CTCTACTGGC TTTAGCCCTT 
CCCGGTATTG ATGCCAACGA TCTCAATAAA ACACAGTATA CGTTGAACTT CATCCAAAGT 
GTGGCGTACA GCATCCCTTT TGTCCCGCTT GTCGATGAGA ATAACGACAT TCATGACACG 
AGCCTTGCCA TGAACTGGGT TCAGGGGGAG ATGGAGAGAA TGGAGCGCGA GGGGCAAGAT 
GTCAAGATCA ACTACAACGA GGAGCTTAGT GACGAAGACG AAGCAAACAT CCTTCGATCC 
TCCACCGCTG GTCTTGGAGA GTTTGTCATG GCTTTACTAG GCAAGGTGTT TGCCCTGCTA 
GAAAACCTTC CAGACGCGAG CCATCTACGA ACAGGCTCCC CAGAGGACAA CGTGATCAAC 
ACGCTCCCTG CTGCACTCAC GCCTTTGTTC GCATCGCTGT CTCCTCAGCT CTTTGACTCG 
GCGTTGGAGA AGCTGGCAAA CTTTGTGTCG ACTCATGTAG TGCATCAAGC TCGGGATGCC 
ATGGCGTGGA TTTGCAATGC CTTGTGCAAG GTCAACCCGG AAAAGACGCT CAAGGTCTTC 
ATTCCCATGC TGATTGTCAA CATTCGCAAT GAGATCGACT ACAACGGTGC TGCTTCAGAT 
CGAAGCAGTG GTACCGAAGT GCTTCCCAGG GACAGAGCGT TGGTCTGGCA TGTGAGCATG 
CTCAGCATGT GCGTGGTCCA CGTCGGCAGT GAGGTTCTCA AATACAAGGA CGAGCTTTTC 
GGAATCGCCA AGTACATGCA GGAAAAATGC CGTGGTCTTC CCACCATTCA CATCTCCAAC 
TACGTCCATC ATTTACTGCT CAACCTTACC CACACCTACC CAATCGATAA TGCTCTGTAT 
GAGCCCGATG TAATTGCTCG CGGCATCGAC GTCAGCGACT GGGGCCGTAC GACTGAGCCA 
TCTGAACTCA CCATCAAGTG GCACCGGCCG TCTCCCGCCG AAGTGGCTTT TGCTGTGGAG 
CTGTTTGAGA GCGAGGCTCG TGGTGCCACG GAGAGACTCA TGCAGCTCAT GAGTGACGAT 
CCTCCGGTTA GCAGAAAGGG AAAGAACAAA GAGTGGTCAG ATGAGTTGTC GCGGAGCTTG 
ACAGCGCTTC GTCTTTTGAT CTCGGGCGTC TCAACCCTTT TCGACCCTGT CCGTGCGTCA 
GGAGAAGCAA CTGGCTCCAG CGGTAACCAG GATACCGATG CCGCCGGTGA CACAATTATG 
GAAGAGGACA ATGACCCGCT GGCTGAGATC GCCGACGATG ATGAACTCAA GCGCACATTC 
CGGTATCCCG CTGGATACGT ATTGACCACG GATGATCCTC TCTACCATCG CGTGCACGAT 
CTTCGCGAGG AGGTGGGTAG GACATTATCG CAAACCCATT CCTTCTTGAA CGCAAACCAG 
CAGGATGATG TTGCATGCTT CACGGCCCTA TATAGCGCAT ACAGGACGTG GATCACTGAC 
GTGGGAATTG AACGTTCTGC TCATCCCTTG GAGCGTTTGA CTCGCTTGTA CAAGGCAGAT 
ATTTCCCCCT TCAAGATTTC GGGTCTGCGA AAGGTTTATC CTCGACCACT GTTGATCAAG 
CGTGCGGATG CGTACCAGCT CCAGCGTGTC AAATACAACT CGGCGTATCG CACCAGCAGT 
GAGCTGGACC GGCGCCTGCT CTTGGATCTT GCCGAGTCTT GCACGTCATC ATACGCGGAT 
GTTCGTCGGG TCGCACAAAG TGCTCAAGAC TCGTCTCTTA AGGTCATCAT TGGTGGAAGG 
CCTTTAGTTA TTCCAGTCAT CCTCAATGCT TTGCGTAAGG CACTGGATGA TATGGATCAT 
GACCGTATCA AGGGCGCCAT GTATACCTTG TTCTTTACTA CTCTGCTCAA GACGTGTATG 
AAAGACTGGC GGTTCGCTCC CGAGCTTATG AGGCTCTACA TCGAAACCGC CAGTGTAGAC 
AAGACGTCTA TTCAGAATCT GGGCGCCACG GCTCTCTACC CGCTCATTGA TTTCGGCAAG 
CCGTTTGAGC GCATGATACT CTTTGATCGG TCGGTCGCTG ACACCATTCG ACCCCCGCAG 
GACTTCTCAG CGTCCATCCA ACGGCGTCAT GAGTTCATTA AAGAACGCCG CGCTAAGGTG 
GAGGAGAAGA AGGCTGCATT AGGGTTGGAA CTTATTAATA AGGCCAAGGC TTCGCACTGG 
AAGGTCGCCA CGAGGTGTGC CATGTTTGCA CTCAACATGT CTCTTCGTTT TGAGACACTA 
GCGCCTCCCG AGTTCATCGA ATTGGTCGTT ACTGGCACGA ATGATCCTCA TCCCGGTCTT 
CGCTCGACTT ATCTGGCTGC ATTCAGTGGG CTATGTACCT CCATAGACAT GAGGGCGGTC 
TACCAGCACA GCTATGAGAA GTACTTGCTA GAGCAAGCTG TCGGAGACAA TAAGTTTGAG 
GTTCCCGTTC CTGAGGACGA TCCTGAGTGG ACTCAAAAGT TTCTTGCCCA GTTCGAGAAC 
ACTGATGAGA TCCAGTACTT TGTCGATAGT GACTATCCTG GTTGGTTGGT GTGGGGCAAA 
AAGTTCATAG CCTCATCCGC GAACCCGGAG CCATTCCTAG GATACGACGA GATCGAGACA 
GCTGCTCGTC AGCAAATCGG AAAGCTTTTG ACCCGCGACT GGTTCAAGCA ATGCTTCGAG 
TACCTCAAGC AGGAACCCAG AGATGCCGGC GCAGACAGAT TCAGAATGTC TAACGTCGTA 
CTTCTCATGC AGGTCTTTGA CCTCATGAAC TATGGTCAGA CAGCCGCCAC GTTTGACGAC 
ATCGTTGAAT TGACCAAGGA GATTTACGGA GATGGCGGTG ACAAGCATCA GCATCGTGCA 
ACGGCAGAAA TCGTCGGAGC CATGCTGTCT GGAAGTAGCG ATGATCCTCG CGAGTTCCGC 
GACAAGGTCT GGGCTTTCGC AGCACCCTTG ATGCTCAAGG TGATCGCAGA CGATTTGACG 
CCGGATAATC TGCAGTACTG GATGACATGT CTCCATCTCA CGGTTGATTC CAAGGACCCT 
AGACGTTCGA AGGAGCTCAT CGACCCTCTG GCGAACTTCC GCCTTGATAT GAACTCCAAT 
GCAGCCTTCA AGGAGTCCGC CAAGATCCAA TTGCTCGAGT TCATCATTAA TGATGCTGGC 
TGGCATTTCC GCCGTGACAA GCCCATTCTT GAAGACTTCC TGGCCCATAT CGACCATCCC 
TACAAGTCTG TTCGAGAATC CATTGGTAGA GTCATTGCGA CGATTTATCG GACCAGATAC 
CATGAGTCCT TCCCCAATGT CTGGTCTCTG CTAGAGCAAA ACAAGAGCGC CTCCTCGATT 
GGTATTCGCC CTTACGAGGC AACTGAAGAC TTTACGGCTA CGATCCGCGA GGTCTTTGAT 
CGCATCGAGA AGTGGCGTCA TGAGCGTACT CCCGGACAGC AGACTCCCTC CAGCTATACG 
TCCGGGTCCA AGACGGTACT TGTGTGGCTG GATAGTATGC TCGCCTCGCA GGAATGCATC 
CAACTTGTGC CCTTCTTTCC CGAGCCGTTT ATTGACCAGC TGTTGCACAT GATGGACGTC 
AAGGAAGACC CTGAACTTAT GCGGCTCGCA TACCACGTTT ACCGGCACAT GCCCAACATC 
CCCTTCCGGT CCGGTGAGGA TGACGCATTC ATTGCCGCTC TGATCCGGGT TGGTAAGACA 
GCGGCCTCAT GGCATCAGCG CCTACGCGCT CTGGTCAACA TGCAAGTCAT CTACTTCCGT 
CGTCTCTTCC TAACCCAACC AGCCCAGCGA CAAATGCTCT TCGACGCTGT CGGCGACATG 
TTGTCCGACG TGCAACTCGA AGTCCGCGAC GGGGCCAGCG CTACACTTGC TGGCATGATA 
AGATGCTCGC CGGAGAGAAT TCGTAACCCC ATTATCGAGC AGCTCAAGGC CCGCTTCCAG 
CTGCAACTGG AGATGAACCC GATGCCCAAG CGCAAGTTGC CTGGCACCGA TACGCCCGTG 
GACGTGCAGA AACAGATTAT CAGACGGCAT GCGGCCGTGC TGGGCTTAGG CGCCCTCATC 
GAGGCGTTCC CCTATGCGAC GCCGCCCCCT AAGTGGATGC CCGAGGTATT GGCGTACTTG 
GCGACGCACG CGGCGAGTGA TCCGGGTGTG GTGGGTAAGG CGACTAAGGG GATTTTGGCG 
GAATTCAAGA AGACGAGGCA GGATAGTTGG ACGGTTGATC AGAAGTACTT TACAAGCGAG 
CAACTGGAGG ATCTGGAGGG TGTGTTGTGG AAGAGTTACT TTGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Nature422(6934)859-68(2003 Apr)
Galagan JE,Calvo SE,Borkovich KA,Selker EU,Read ND,Jaffe D,FitzHugh W,Ma LJ,Smirnov S,Purcell S,Rehman B,Elkins T,Engels R,Wang S,Nielsen CB,Butler J,Endrizzi M,Qui D,Ianakiev P,Bell-Pedersen D,Nelson MA,Werner-Washburne M,Selitrennikoff CP,Kinsey JA,Braun EL,Zelter A,Schulte U,Kothe GO,Jedd G,Mewes W,Staben C,Marcotte E,Greenberg D,Roy A,Foley K,Naylor J,Stange-Thomann N,Barrett R,Gnerre S,Kamal M,Kamvysselis M,Mauceli E,Bielke C,Rudd S,Frishman D,Krystofova S,Rasmussen C,Metzenberg RL,Perkins DD,Kroken S,Cogoni C,Macino G,Catcheside D,Li W,Pratt RJ,Osmani SA,DeSouza CP,Glass L,Orbach MJ,Berglund JA,Voelker R,Yarden O,Plamann M,Seiler S,Dunlap J,Radford A,Aramayo R,Natvig DO,Alex LA,Mannhaupt G,Ebbole DJ,Freitag M,Paulsen I,Sachs MS,Lander ES,Nusbaum C,Birren B

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NCU05620 cDNA ORF clone, Neurospora crassa OR74A

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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