NCU09063 cDNA ORF clone, Neurospora crassa OR74A

Gene
Clones
gRNAs

Summary

Gene Symbol	NCU09063
Entrez Gene ID	3874714
Full Name	nuclear condensin complex subunit Smc4
Gene Type	protein-coding
Organism	Neurospora crassa OR74A

mRNA and Protein(s)

mRNA	Protein	Name
XM_953474.3	XP_958567.2	nuclear condensin complex subunit Smc4

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	SMC4	XP_005639913.1
Eremothecium gossypii	AGOS_AGR089C	NP_986754.1
Neurospora crassa	NCU09063	XP_958567.2
Gallus gallus (chicken)	SMC4	NP_989849.1
Schizosaccharomyces pombe (fission yeast)	cut3	NP_595392.1
Magnaporthe oryzae (rice blast fungus)	MGG_03378	XP_003716567.1
Oryza sativa (rice)	Os05g0497100	NP_001174473.1
Bos taurus (cattle)	SMC4	NP_001098796.2
Rattus norvegicus (Norway rat)	Smc4	NP_001032262.2
Kluyveromyces lactis	KLLA0F19085g	XP_455936.1
Xenopus tropicalis (tropical clawed frog)	smc4	XP_002933283.1
Danio rerio (zebrafish)	smc4	NP_775360.1
Arabidopsis thaliana (thale cress)	SMC3	NP_001190492.1
Homo sapiens (human)	SMC4	NP_005487.3
Pan troglodytes (chimpanzee)	SMC4	XP_003950273.1
	SMC4	XP_001098806.2
Mus musculus (house mouse)	Smc4	NP_598547.1
Drosophila melanogaster (fruit fly)	glu	NP_723996.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007826	XP_317674.2
Saccharomyces cerevisiae (baker's yeast)	SMC4	NP_013187.1

Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Galagan JE, Calvo SE, Borkovich KA, Selker EU, Read ND, Jaffe D, FitzHugh W, Ma LJ, Smirnov S, Purcell S, Rehman B, Elkins T, Engels R, Wang S, Nielsen CB, Butler J, Endrizzi M, Qui D, Ianakiev P, Bell-Pedersen D, Nelson MA, Werner-Washburne M, Selitrennikoff CP, Kinsey JA, Braun EL, Zelter A, Schulte U, Kothe GO, Jedd G, Mewes W, Staben C, Marcotte E, Greenberg D, Roy A, Foley K, Naylor J, Stange-Thomann N, Barrett R, Gnerre S, Kamal M, Kamvysselis M, Mauceli E, Bielke C, Rudd S, Frishman D, Krystofova S, Rasmussen C, Metzenberg RL, Perkins DD, Kroken S, Cogoni C, Macino G, Catcheside D, Li W, Pratt RJ, Osmani SA, DeSouza CP, Glass L, Orbach MJ, Berglund JA, Voelker R, Yarden O, Plamann M, Seiler S, Dunlap J, Radford A, Aramayo R, Natvig DO, Alex LA, Mannhaupt G, Ebbole DJ, Freitag M, Paulsen I, Sachs MS, Lander ES, Nusbaum C, Birren B
Nature422(6934)859-68(2003 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following NCU09063 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NCU09063 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ONf00535	XM_953474.3 Latest version!	Neurospora crassa OR74A nuclear condensin complex subunit Smc4 (NCU09063), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NCU09063 ( XM_953474.3 ) cDNA ORF clone, Neurospora crassa OR74A -> XP_958567.2 Neurospora crassa OR74A nuclear condensin complex subunit Smc4 (NCU09063), mRNA.

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CloneID	ONf00535
Clone ID Related Accession (Same CDS sequence)	XM_953474.3
Accession Version	XM_953474.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4953bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-10
Organism	Neurospora crassa OR74A
Product	nuclear condensin complex subunit Smc4
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026507). On Feb 23, 2015 this sequence version replaced XM_953474.2.

ATGTCGACGC GCCCATCGCG TCGGGCCGCC GCAACCAGGC GCGCCGCCAT CATCGAAGAA 
TCCTCATCAG ACGACGATAT CGCAAACACC ACATCGAGAG GCGCCCAAGA TGACGACGAG 
GAGGACGATT ATACACCCGC GCCAGCTTCG GCAGCCCGCA AGACTGCTCA GCGAACCACC 
AGAAGATCCA CTGCCACGCC CGCTCCTAGG GCAGGAACAC CAGCAGCAGC ATCATCATCA 
ACTGCACCGG CACCGGCACC AAAAGCTGCG CCGAAAAGGC GCGGACGACC TAAGAAGAAT 
GCCGCGCCCA AACCCAGCAA AGAGCCCAGT GTCGAACCGA GTGTCGAAGC GAATAACGAT 
CCCCAAGCCG AACCCGAAGC CCCAGCGCCT GCCGTCAAGC CAAAGGATGA GCCGATCGAG 
GAGGGTATCG ACCCCAATGT GACTGTCGCG TCTGCCGTTT CGCCCGCTAC AACCGCCGTG 
ATCGCGGAGC CAGAAGCACG GGATACGCAA GAAATTCAGT CACCCCCGCG GAAGCGTAGA 
ACTCTTGTGC GGCGCCTCAG CAGATCGCCA GCAAAGATCA TGCAGCAACA ACTCGAGCTG 
CAACAGCAAC AAGCCAGCCG TCAAGGGACA CCAACGCCAG GCTCTCCGTC AACTCAACGC 
GTCGATCGCC AACCTGCTCC TCTACAGGAC ATCACCCAGA CCCTCATCAA CCTACCACCT 
CCAGCGACCG CGGCATCAGA GACCCTTGTC AACACACAGC CAACATCAAC AGCAGATGAA 
ACCGTCGTGG CGGCCCAGAA GCCCGTCAGG GCTATGGATA CTATTCTGGA AAAGCCCATG 
GATATTGTGC TCAAGTCACG GACCATGGCT CTTCCCGTCG TCGAGGATAC CGGCCCGAAG 
CCACGCATTG TCATCACCAA TCTTGTGCTC ATCAACTTCA AGAGTTACGC CGGTAGACAG 
GAGGTCGGCC CCTTCCATGC TTCGTTCTCT TCGGTCGTGG GTCCCAACGG TTCTGGCAAA 
TCCAACGTCA TCGACTCGTT GCTGTTCGTC TTTGGTTTCC GTGCCAGCAA GATGCGACAA 
GGCAAGATCT CGGCCTTGAT TCACAATTCG GCGCAGTTTC CGAATCTCGA CTACTGTGAG 
GTGGAAGTGC ATTTCCAGGA AGTCATGGAC TTGCCCGGCG GTGGCCACGA GGTTATCCCC 
GACTCAACAC TCGTCATCTC GCGCAAAGCA TTCAAGAACA ACTCGAGCCA GTACTACATC 
AACGGCAAAT CATCCAATTT TACCACCGTG ACCACGCTAC TCCGCGACCG TGGTGTCGAT 
CTGGATCACA AGCGTTTTCT CATTCTCCAG GGCGAAGTCG AATCGATTGC CCAGATGAAG 
CCCAAAGCCG GCAACGAGCA CGAAGATGGT CTGTTGGAGT ACCTAGAAGA CATTATTGGC 
ACTTCCAAAT ACAAGACACC AATTGAAGAG TCGGCCACCG AAGTCGAAAC CCTCAACGAG 
ATCTGCATGG AGAAGAGTGG TCGTGTCCAG CACGTGGAAA AGGAAAGGAA CAGTCTCGAG 
GACAAGAAGA ACAAGGCGCT GGCGTATGTT CGCGATGAAA ACGAACTGGC GCTGAAACAG 
TCGGCTCTCT ACCAGCTATA CATCAGCGAA TGCGACGACA ATATTGCTGT CACAGAGGAG 
GCTATCGGTC AGATGCAGGC ACAACTTGAC GCTGAGCTTG AGAAGCACCA CGGCAGCGAA 
CAGCTCATCA AGCAGTTGGA AAAGGCCTAC TCCAAGGGTA GCAAGGAATA CGACTCTTGG 
GAGAAGCAGA CACAGCAGCT GGTCAAGGAG ATGAACAAGT TCGAGCAGGA ACGCGTCAAA 
TTCGAGGAGA AGCGGAAGTT CTTGACGGAC AAGCGTAAGA AGCTTGAGAA GACGATCGCT 
AACGCCGAGA AGACGGCTGT TGAAGCGGAG GAGACCATTC AGAACTGCAC CAAGGAGATT 
GAGCGTCGTC AAAAGGACAT TGTTTTCCTG GAGAAGAAGG TCAAGGAAGA GGAGGCCGAG 
CTTGCGACGA TACGTGAGAG CCTCAAGGGC AAGACGCAAA AGTACTCGGA TCAGATCGCG 
GCAAAGCAAA AGTCTCTCGA GCCTTGGAAG GACAAGATCA ATCAGAAACA GTCCGCCATT 
GCCGTTGCGG AGAGTGAGCT GGCCATTCTC AGGGAAAAGG CCAATGCCGG AACTGTGGCC 
CTTGAGGAGA TGGAGACGAA AATTGTGGCC ATCCAGGAAA GGCAAGCGGC GAAGGCGGAG 
GAGATGGAGC AGTGCAAAGC TGAGAGAGCG AACCTGATCA AGGAGGGCAA GAAGATCGAG 
GCTGAACTTT CCAAGCTTGC GGAGGAGGAA CCCAAGTACC GGCAACAGCT TTCGAATGCC 
CGCCAAAAGG CTGACGAGGC GCGGTCGAGC TTGTCCGCCA CACAAAACCG AGGCAATGTC 
TTGACTGCCC TTATGCGCAT GAAGGAGTCG GGTCGTATCG ATGGGTTTCA TGGACGACTT 
GGAAATCTCG GTGCTATTGA CCAGAAGTAC GATATTGCCA TCTCTACAGC CTGTGGTGCT 
CTGGACAACT TTGTCACCGA CACTGTTGAG GCCGGTCAGC AATGTATTGA ATATCTCCGA 
AAAACCAACC TCGGACGTGG TAATTTTATG TGCTTGGACA AGTTGCGAGT CCGCGACATG 
TCTCCTATTC AGACTCCCGA AAACGCCCCT CGTCTCTTTG ACCTTGTCAA GCCCAAAGAC 
GACAAGTTCC GACCTGCCTT CTACCATGCT CTTCAGGACA CATTGGTCGC CAAGGATCTC 
GCTCAGGCAA ACCGCATTGC CTATGGCGCT AAGAGATGGC GAGTAGTGAC CCTTGCCGGC 
GAGCTCATCG ATAAGTCAGG TACCATGACC GGTGGTGGCA CTACAGTCAA GCGGGGTCTG 
ATGTCCTCCA AGCTTGTGGC TGACACCACC AAGGAGCAAG TTGACAAGCT TGAGGCGGAC 
CGTGATACCT TGGAGCAGGC CTTCAACGAG TTCCAAGACC GCCAACGAGA ACTCGAGTCC 
CGTCTGCGCC AGATCCAGTC CATGATTCCC CAGCTTGAGA CCAAGATGCA GAAGATCAAC 
CTCGAAGTTG AGAGCTCGGA GCGCAACCTT GCCGATGCCC AGCGCCGTAT CAAGGAGCTC 
AGCAAGGAGC AACAGCCTTC CAAGACGGAT GACAACCGCG TCGCCGCCCT TGAAAAGGAA 
ATTGCCAAGC TTGAGGGCGA GGTCGAGAAG CTGCGCGAGG AGATGTCCTC CGTCGAGGAT 
GAAATTAAGG CTCTTCAAGA TAAGATCATG AAGGTAGGTG GTGAAAAGCT TCGCTCTCAA 
AAAGCCGCTG TGGACTCGTT AAAGGCAGAG ATCGCCACGC AAAACGAGGA AACTTCTGCC 
GCCGAGGTGA AGATGGCGAA AGCGGAGAAA CAGAAGCACA AGCTAGAGAA GGACTTTAAC 
AAGGCGTCTA AAGAGCTGCA GAATGCCATT CAGGAGACCG AGGCTCTGGA AGAGGAGATT 
CAGAACCAGG GCGATACTGC CGAGAGCCTC ACGACCCGTG TTGAAGAGGC CCAGGAGGCA 
CTCAAGGAGA AGAAGAAAGA ACTGGCAGCT ATGAAGAAGG AGCTGGATGA GAAGACGGCC 
GAGCTCAACG AGACAAGGGC GGTGGAAATT GAGATGCGCA ATAAGCTCGA GGAAAACCAA 
AAGATCCTGT CTGAGAACCA GAAGCGTCTG CGCTACTGGG AAGACAAGCT CTCCAAGCTC 
GCCCTCCAAG ACATTGAAGA TCTCGAGCGC GGCACCAGCG GCGACGCAGC AGCACCTCAA 
CAACTTGCCG ACCCTGATGC CATGGACGTC GACGACCCTG ATTCCATTCT CCAAGCCCAA 
CTCGAGGCCG AAGCCGCCGA GGCCGAAGAG AACGAGGAGG ACGAGGAGGA AGAGGAGGAG 
GAGGAAGACG AAGATCCAGA AGCCATGTCC CTCGACGAAG ACGACGAGGA ACCTCGCCAG 
CGCAAGCAGA AAACCCCCAA GAAGCAAAAG CAAAAGCGCC GTCAACAAAC CGGCAAAACC 
CCGCGCAAAC CCCGCAACCC CCTCGAACTC CCCCGCTACA CCCGCGACGA GCTAGCCGAC 
ATGTCCAAAG AGAAACTCAA GGGCGAGATC GCCGCGCTCG AAGAGAAGAT CTCCAACGTG 
AACATCGACC TGTCTGTACT CTCCGAATAC CGTCGTCGCG TGGAAGAACA TCTCGCGCGT 
TCTTCGGACT TGGCCTCAGC CGTCTCCCAG CGCGACGCCG CCAAGAAGCG TCTCGACGAG 
CTCCGTCGTC TGCGATTGGA GGGCTTCATG GAAGGGTTCT CGACCATCTC TCTTAGACTA 
AAAGAGATGT ACCAGATGAT CACCATGGGC GGCAACGCAG AGCTGGAGCT GGTGGACAGT 
CTGGACCCCT TCTCGGAGGG AATCTTGTTC AGCGTGATGC CGCCCAAGAA GAGCTGGAAG 
AATATTTCTA ATCTAAGTGG TGGCGAGAAG ACGTTGAGTT CTCTGGCGCT GGTGTTTGCA 
TTGCATCATT ACAAGCCGAC GCCGCTTTAT GTGATGGATG AAATTGATGC GGCGCTGGAT 
TTCAGGAATG TCTCCATCGT AGCCTCCTAC ATCAAAGAAC GCACCAAAAA CGCCCAATTC 
ATCGTCATCT CCCTGCGCAA CAACATGTTT GAGCTCGCCG CCCGTCTGGT CGGCGTCTAC 
AAGGTCAACC ACATGACCAA GTCGGTCACA ATTGAGAATC ATGATTATAT CCAGCGAGGC 
AAACATCAGA AGGAGGAAAA AGAACGGAGG GAGAGGGAGA GACGCCAGAA AGAAGAGGAG 
AAGAGGGAAA GGCAAGAAAG GGAACAAAGA GAGAGGGAAA TGATGGGACA ACAGTCGGCT 
GCGGAGGTTA CTGTTGCTAG TATTGAGGCT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_958567.2
CDS	302..5254
Translation	MSTRPSRRAAATRRAAIIEESSSDDDIANTTSRGAQDDDEEDDYTPAPASAARKTAQRTTRRSTATPAPRAGTPAAASSSTAPAPAPKAAPKRRGRPKKNAAPKPSKEPSVEPSVEANNDPQAEPEAPAPAVKPKDEPIEEGIDPNVTVASAVSPATTAVIAEPEARDTQEIQSPPRKRRTLVRRLSRSPAKIMQQQLELQQQQASRQGTPTPGSPSTQRVDRQPAPLQDITQTLINLPPPATAASETLVNTQPTSTADETVVAAQKPVRAMDTILEKPMDIVLKSRTMALPVVEDTGPKPRIVITNLVLINFKSYAGRQEVGPFHASFSSVVGPNGSGKSNVIDSLLFVFGFRASKMRQGKISALIHNSAQFPNLDYCEVEVHFQEVMDLPGGGHEVIPDSTLVISRKAFKNNSSQYYINGKSSNFTTVTTLLRDRGVDLDHKRFLILQGEVESIAQMKPKAGNEHEDGLLEYLEDIIGTSKYKTPIEESATEVETLNEICMEKSGRVQHVEKERNSLEDKKNKALAYVRDENELALKQSALYQLYISECDDNIAVTEEAIGQMQAQLDAELEKHHGSEQLIKQLEKAYSKGSKEYDSWEKQTQQLVKEMNKFEQERVKFEEKRKFLTDKRKKLEKTIANAEKTAVEAEETIQNCTKEIERRQKDIVFLEKKVKEEEAELATIRESLKGKTQKYSDQIAAKQKSLEPWKDKINQKQSAIAVAESELAILREKANAGTVALEEMETKIVAIQERQAAKAEEMEQCKAERANLIKEGKKIEAELSKLAEEEPKYRQQLSNARQKADEARSSLSATQNRGNVLTALMRMKESGRIDGFHGRLGNLGAIDQKYDIAISTACGALDNFVTDTVEAGQQCIEYLRKTNLGRGNFMCLDKLRVRDMSPIQTPENAPRLFDLVKPKDDKFRPAFYHALQDTLVAKDLAQANRIAYGAKRWRVVTLAGELIDKSGTMTGGGTTVKRGLMSSKLVADTTKEQVDKLEADRDTLEQAFNEFQDRQRELESRLRQIQSMIPQLETKMQKINLEVESSERNLADAQRRIKELSKEQQPSKTDDNRVAALEKEIAKLEGEVEKLREEMSSVEDEIKALQDKIMKVGGEKLRSQKAAVDSLKAEIATQNEETSAAEVKMAKAEKQKHKLEKDFNKASKELQNAIQETEALEEEIQNQGDTAESLTTRVEEAQEALKEKKKELAAMKKELDEKTAELNETRAVEIEMRNKLEENQKILSENQKRLRYWEDKLSKLALQDIEDLERGTSGDAAAPQQLADPDAMDVDDPDSILQAQLEAEAAEAEENEEDEEEEEEEEDEDPEAMSLDEDDEEPRQRKQKTPKKQKQKRRQQTGKTPRKPRNPLELPRYTRDELADMSKEKLKGEIAALEEKISNVNIDLSVLSEYRRRVEEHLARSSDLASAVSQRDAAKKRLDELRRLRLEGFMEGFSTISLRLKEMYQMITMGGNAELELVDSLDPFSEGILFSVMPPKKSWKNISNLSGGEKTLSSLALVFALHHYKPTPLYVMDEIDAALDFRNVSIVASYIKERTKNAQFIVISLRNNMFELAARLVGVYKVNHMTKSVTIENHDYIQRGKHQKEEKERRERERRQKEEEKRERQEREQREREMMGQQSAAEVTVASIEA

Target ORF information:

RefSeq Version	XM_953474.3
Organism	Neurospora crassa OR74A
Definition	Neurospora crassa OR74A nuclear condensin complex subunit Smc4 (NCU09063), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_953474.3

ORF Insert Sequence:

ATGTCGACGC GCCCATCGCG TCGGGCCGCC GCAACCAGGC GCGCCGCCAT CATCGAAGAA 
TCCTCATCAG ACGACGATAT CGCAAACACC ACATCGAGAG GCGCCCAAGA TGACGACGAG 
GAGGACGATT ATACACCCGC GCCAGCTTCG GCAGCCCGCA AGACTGCTCA GCGAACCACC 
AGAAGATCCA CTGCCACGCC CGCTCCTAGG GCAGGAACAC CAGCAGCAGC ATCATCATCA 
ACTGCACCGG CACCGGCACC AAAAGCTGCG CCGAAAAGGC GCGGACGACC TAAGAAGAAT 
GCCGCGCCCA AACCCAGCAA AGAGCCCAGT GTCGAACCGA GTGTCGAAGC GAATAACGAT 
CCCCAAGCCG AACCCGAAGC CCCAGCGCCT GCCGTCAAGC CAAAGGATGA GCCGATCGAG 
GAGGGTATCG ACCCCAATGT GACTGTCGCG TCTGCCGTTT CGCCCGCTAC AACCGCCGTG 
ATCGCGGAGC CAGAAGCACG GGATACGCAA GAAATTCAGT CACCCCCGCG GAAGCGTAGA 
ACTCTTGTGC GGCGCCTCAG CAGATCGCCA GCAAAGATCA TGCAGCAACA ACTCGAGCTG 
CAACAGCAAC AAGCCAGCCG TCAAGGGACA CCAACGCCAG GCTCTCCGTC AACTCAACGC 
GTCGATCGCC AACCTGCTCC TCTACAGGAC ATCACCCAGA CCCTCATCAA CCTACCACCT 
CCAGCGACCG CGGCATCAGA GACCCTTGTC AACACACAGC CAACATCAAC AGCAGATGAA 
ACCGTCGTGG CGGCCCAGAA GCCCGTCAGG GCTATGGATA CTATTCTGGA AAAGCCCATG 
GATATTGTGC TCAAGTCACG GACCATGGCT CTTCCCGTCG TCGAGGATAC CGGCCCGAAG 
CCACGCATTG TCATCACCAA TCTTGTGCTC ATCAACTTCA AGAGTTACGC CGGTAGACAG 
GAGGTCGGCC CCTTCCATGC TTCGTTCTCT TCGGTCGTGG GTCCCAACGG TTCTGGCAAA 
TCCAACGTCA TCGACTCGTT GCTGTTCGTC TTTGGTTTCC GTGCCAGCAA GATGCGACAA 
GGCAAGATCT CGGCCTTGAT TCACAATTCG GCGCAGTTTC CGAATCTCGA CTACTGTGAG 
GTGGAAGTGC ATTTCCAGGA AGTCATGGAC TTGCCCGGCG GTGGCCACGA GGTTATCCCC 
GACTCAACAC TCGTCATCTC GCGCAAAGCA TTCAAGAACA ACTCGAGCCA GTACTACATC 
AACGGCAAAT CATCCAATTT TACCACCGTG ACCACGCTAC TCCGCGACCG TGGTGTCGAT 
CTGGATCACA AGCGTTTTCT CATTCTCCAG GGCGAAGTCG AATCGATTGC CCAGATGAAG 
CCCAAAGCCG GCAACGAGCA CGAAGATGGT CTGTTGGAGT ACCTAGAAGA CATTATTGGC 
ACTTCCAAAT ACAAGACACC AATTGAAGAG TCGGCCACCG AAGTCGAAAC CCTCAACGAG 
ATCTGCATGG AGAAGAGTGG TCGTGTCCAG CACGTGGAAA AGGAAAGGAA CAGTCTCGAG 
GACAAGAAGA ACAAGGCGCT GGCGTATGTT CGCGATGAAA ACGAACTGGC GCTGAAACAG 
TCGGCTCTCT ACCAGCTATA CATCAGCGAA TGCGACGACA ATATTGCTGT CACAGAGGAG 
GCTATCGGTC AGATGCAGGC ACAACTTGAC GCTGAGCTTG AGAAGCACCA CGGCAGCGAA 
CAGCTCATCA AGCAGTTGGA AAAGGCCTAC TCCAAGGGTA GCAAGGAATA CGACTCTTGG 
GAGAAGCAGA CACAGCAGCT GGTCAAGGAG ATGAACAAGT TCGAGCAGGA ACGCGTCAAA 
TTCGAGGAGA AGCGGAAGTT CTTGACGGAC AAGCGTAAGA AGCTTGAGAA GACGATCGCT 
AACGCCGAGA AGACGGCTGT TGAAGCGGAG GAGACCATTC AGAACTGCAC CAAGGAGATT 
GAGCGTCGTC AAAAGGACAT TGTTTTCCTG GAGAAGAAGG TCAAGGAAGA GGAGGCCGAG 
CTTGCGACGA TACGTGAGAG CCTCAAGGGC AAGACGCAAA AGTACTCGGA TCAGATCGCG 
GCAAAGCAAA AGTCTCTCGA GCCTTGGAAG GACAAGATCA ATCAGAAACA GTCCGCCATT 
GCCGTTGCGG AGAGTGAGCT GGCCATTCTC AGGGAAAAGG CCAATGCCGG AACTGTGGCC 
CTTGAGGAGA TGGAGACGAA AATTGTGGCC ATCCAGGAAA GGCAAGCGGC GAAGGCGGAG 
GAGATGGAGC AGTGCAAAGC TGAGAGAGCG AACCTGATCA AGGAGGGCAA GAAGATCGAG 
GCTGAACTTT CCAAGCTTGC GGAGGAGGAA CCCAAGTACC GGCAACAGCT TTCGAATGCC 
CGCCAAAAGG CTGACGAGGC GCGGTCGAGC TTGTCCGCCA CACAAAACCG AGGCAATGTC 
TTGACTGCCC TTATGCGCAT GAAGGAGTCG GGTCGTATCG ATGGGTTTCA TGGACGACTT 
GGAAATCTCG GTGCTATTGA CCAGAAGTAC GATATTGCCA TCTCTACAGC CTGTGGTGCT 
CTGGACAACT TTGTCACCGA CACTGTTGAG GCCGGTCAGC AATGTATTGA ATATCTCCGA 
AAAACCAACC TCGGACGTGG TAATTTTATG TGCTTGGACA AGTTGCGAGT CCGCGACATG 
TCTCCTATTC AGACTCCCGA AAACGCCCCT CGTCTCTTTG ACCTTGTCAA GCCCAAAGAC 
GACAAGTTCC GACCTGCCTT CTACCATGCT CTTCAGGACA CATTGGTCGC CAAGGATCTC 
GCTCAGGCAA ACCGCATTGC CTATGGCGCT AAGAGATGGC GAGTAGTGAC CCTTGCCGGC 
GAGCTCATCG ATAAGTCAGG TACCATGACC GGTGGTGGCA CTACAGTCAA GCGGGGTCTG 
ATGTCCTCCA AGCTTGTGGC TGACACCACC AAGGAGCAAG TTGACAAGCT TGAGGCGGAC 
CGTGATACCT TGGAGCAGGC CTTCAACGAG TTCCAAGACC GCCAACGAGA ACTCGAGTCC 
CGTCTGCGCC AGATCCAGTC CATGATTCCC CAGCTTGAGA CCAAGATGCA GAAGATCAAC 
CTCGAAGTTG AGAGCTCGGA GCGCAACCTT GCCGATGCCC AGCGCCGTAT CAAGGAGCTC 
AGCAAGGAGC AACAGCCTTC CAAGACGGAT GACAACCGCG TCGCCGCCCT TGAAAAGGAA 
ATTGCCAAGC TTGAGGGCGA GGTCGAGAAG CTGCGCGAGG AGATGTCCTC CGTCGAGGAT 
GAAATTAAGG CTCTTCAAGA TAAGATCATG AAGGTAGGTG GTGAAAAGCT TCGCTCTCAA 
AAAGCCGCTG TGGACTCGTT AAAGGCAGAG ATCGCCACGC AAAACGAGGA AACTTCTGCC 
GCCGAGGTGA AGATGGCGAA AGCGGAGAAA CAGAAGCACA AGCTAGAGAA GGACTTTAAC 
AAGGCGTCTA AAGAGCTGCA GAATGCCATT CAGGAGACCG AGGCTCTGGA AGAGGAGATT 
CAGAACCAGG GCGATACTGC CGAGAGCCTC ACGACCCGTG TTGAAGAGGC CCAGGAGGCA 
CTCAAGGAGA AGAAGAAAGA ACTGGCAGCT ATGAAGAAGG AGCTGGATGA GAAGACGGCC 
GAGCTCAACG AGACAAGGGC GGTGGAAATT GAGATGCGCA ATAAGCTCGA GGAAAACCAA 
AAGATCCTGT CTGAGAACCA GAAGCGTCTG CGCTACTGGG AAGACAAGCT CTCCAAGCTC 
GCCCTCCAAG ACATTGAAGA TCTCGAGCGC GGCACCAGCG GCGACGCAGC AGCACCTCAA 
CAACTTGCCG ACCCTGATGC CATGGACGTC GACGACCCTG ATTCCATTCT CCAAGCCCAA 
CTCGAGGCCG AAGCCGCCGA GGCCGAAGAG AACGAGGAGG ACGAGGAGGA AGAGGAGGAG 
GAGGAAGACG AAGATCCAGA AGCCATGTCC CTCGACGAAG ACGACGAGGA ACCTCGCCAG 
CGCAAGCAGA AAACCCCCAA GAAGCAAAAG CAAAAGCGCC GTCAACAAAC CGGCAAAACC 
CCGCGCAAAC CCCGCAACCC CCTCGAACTC CCCCGCTACA CCCGCGACGA GCTAGCCGAC 
ATGTCCAAAG AGAAACTCAA GGGCGAGATC GCCGCGCTCG AAGAGAAGAT CTCCAACGTG 
AACATCGACC TGTCTGTACT CTCCGAATAC CGTCGTCGCG TGGAAGAACA TCTCGCGCGT 
TCTTCGGACT TGGCCTCAGC CGTCTCCCAG CGCGACGCCG CCAAGAAGCG TCTCGACGAG 
CTCCGTCGTC TGCGATTGGA GGGCTTCATG GAAGGGTTCT CGACCATCTC TCTTAGACTA 
AAAGAGATGT ACCAGATGAT CACCATGGGC GGCAACGCAG AGCTGGAGCT GGTGGACAGT 
CTGGACCCCT TCTCGGAGGG AATCTTGTTC AGCGTGATGC CGCCCAAGAA GAGCTGGAAG 
AATATTTCTA ATCTAAGTGG TGGCGAGAAG ACGTTGAGTT CTCTGGCGCT GGTGTTTGCA 
TTGCATCATT ACAAGCCGAC GCCGCTTTAT GTGATGGATG AAATTGATGC GGCGCTGGAT 
TTCAGGAATG TCTCCATCGT AGCCTCCTAC ATCAAAGAAC GCACCAAAAA CGCCCAATTC 
ATCGTCATCT CCCTGCGCAA CAACATGTTT GAGCTCGCCG CCCGTCTGGT CGGCGTCTAC 
AAGGTCAACC ACATGACCAA GTCGGTCACA ATTGAGAATC ATGATTATAT CCAGCGAGGC 
AAACATCAGA AGGAGGAAAA AGAACGGAGG GAGAGGGAGA GACGCCAGAA AGAAGAGGAG 
AAGAGGGAAA GGCAAGAAAG GGAACAAAGA GAGAGGGAAA TGATGGGACA ACAGTCGGCT 
GCGGAGGTTA CTGTTGCTAG TATTGAGGCT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Nature422(6934)859-68(2003 Apr)
Galagan JE,Calvo SE,Borkovich KA,Selker EU,Read ND,Jaffe D,FitzHugh W,Ma LJ,Smirnov S,Purcell S,Rehman B,Elkins T,Engels R,Wang S,Nielsen CB,Butler J,Endrizzi M,Qui D,Ianakiev P,Bell-Pedersen D,Nelson MA,Werner-Washburne M,Selitrennikoff CP,Kinsey JA,Braun EL,Zelter A,Schulte U,Kothe GO,Jedd G,Mewes W,Staben C,Marcotte E,Greenberg D,Roy A,Foley K,Naylor J,Stange-Thomann N,Barrett R,Gnerre S,Kamal M,Kamvysselis M,Mauceli E,Bielke C,Rudd S,Frishman D,Krystofova S,Rasmussen C,Metzenberg RL,Perkins DD,Kroken S,Cogoni C,Macino G,Catcheside D,Li W,Pratt RJ,Osmani SA,DeSouza CP,Glass L,Orbach MJ,Berglund JA,Voelker R,Yarden O,Plamann M,Seiler S,Dunlap J,Radford A,Aramayo R,Natvig DO,Alex LA,Mannhaupt G,Ebbole DJ,Freitag M,Paulsen I,Sachs MS,Lander ES,Nusbaum C,Birren B

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NCU09063 cDNA ORF clone, Neurospora crassa OR74A

Gene

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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