ORF » Species Summary » Neurospora crassa OR74A » NCU09118 cDNA ORF clone

NCU09118 cDNA ORF clone, Neurospora crassa OR74A

  • Gene

  • Clones

  • gRNAs

The following NCU09118 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NCU09118 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ONf10611 XM_011394939.1
Latest version!		 Neurospora crassa OR74A DNA topoisomerase I, variant (NCU09118), mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
ONf10612 XM_011394940.1
Latest version!		 Neurospora crassa OR74A DNA topoisomerase I (NCU09118), mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    NCU09118 ( XM_011394939.1 ) cDNA ORF clone, Neurospora crassa OR74A -> XP_011393241.1 Neurospora crassa OR74A DNA topoisomerase I, variant (NCU09118), mRNA.
    Price & Availability↑
    CloneID ONf10611
    Clone ID Related Accession (Same CDS sequence) XM_011394939.1
    Accession Version XM_011394939.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2682bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-10
    Organism Neurospora crassa OR74A
    Product DNA topoisomerase I, variant
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026501). 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGGACGCTG CTGATTCCAA GGTTCACATG GCTCCCGTTG GTGTCTCCAT CCGTAATGGC 
    CCCGTCGACC AGGACAGGAT GGACGTCGAC TCCTCAGTAA GCGGTCAGGC TAAGCGCAAA 
    TCGCGGACAT CCATTGATCA GTCCATCAAC TATAAGGATG ACTCCAGTGA TGACGAAGCA 
    CCCTTGGCGA AACGTTCAAA AACACAACAT AAGCAACCAG ACTCCGATAG CGACGACGAG 
    CCGATTGCGA AAAAAAATGG CGCGAGATTG CCTCCCTCCG CCAAGAATAC GGATATGGAC 
    GTAGATTCTG ATGACGACGA ACCTCTCGGA AAGATCGCCG CGAAGAAGGC CAATATCGAG 
    AAGGAAGCCG CCAAGGAAGC CAAGAGTATT CGTGCTGGTA CCAAGGCCAA GAAGCCTGCT 
    GCTAAGAAGG CCGTCAAGGA TGAATCCTCC GATGACGAAC CCCTTGCGAA ACCCACCAAG 
    CGACAGGTCA ACGGCACAGC TGCGAAGAAG ACGAATGGTG TGAAGAAGGA AGAGTCTGAC 
    TCGGATGTGC CCATCGCTAA GCGGGCGAAG AAGGCACCTG CCGCGAAGCC TGCTGCAAAG 
    CCTGCTGCAA AGCCGGCTGC AAAGGCTGCC ACGAAGGCCG CCCCGAAGGG CAAGGTTGCC 
    GCTGTCAAGA AGTCCGTCAA GGAGGAATCG AAGGACATCA CCGATGAGGA CGATGAGGAA 
    GAGGAGTATA AGTGGTGGGA GGAGTCGGCA AAGAAGGAAG ATGACAGTAT CAAGTGGACC 
    ACCTTGGAGC ACAACGGCGT CATCTTCCCC GAGGATTACC AGCGTTTGCC CAAGGACGTC 
    AAACTGCTCT ACGATGGAAC GCCGGTCGAA CTCGATATCG AGGCCGAGGA AGCTGCGACC 
    TTTTTCGGCT TCATGCTTAA CTCGACGGTG AATGTTGAGA ACCCAACTTT CCAGAAGAAC 
    TTCTTCAGGG ACTTCAAGGA GATCCTAGAT CGGACAGGTG GCGCCAAGGA CAAGAATGGC 
    AAAAAGGTTG ACATCAAGGA ATTCGAAAAA CTCGACTTCT CCCAGTTGTG GGCTTACAAA 
    GATGCTCAAA CGAAGGCTAA GAATGCCCGG TCGTCAGCAG AGAAGAAGGC CGAGAAAGCC 
    GAAAGGGACA AGAGGGACGC CCCCTATCTA TATTGCAAGT GGGATGGGCG AAAAGAAAAG 
    GTTGGTAACC CCAGAGTGGA AATTCCAGGA CTTTTCCGTG GTCGCGGTGA TCACCCCAAG 
    ACCGGGTGTA TCAAGAAGCG TGTTTTGCCA GAGCAAATTA CCATCAACAT CGGCAAAGAG 
    GCCAAGGTCC CAGAGCCGCC AGCTGGCCAC AAGTGGAAGG CCGTTCAACA TGACAACAAG 
    GCAACTTGGC TCGCGATGTG GCAAGAGAAC ATCAACGGAC ACTACAAGTA TGTGATGTTG 
    GCAGCAAACA GTACTGTGAA GGGCAAGGCC GATTTCAAGA AGTTCGAAAA GGCCCGTGAG 
    CTCAAGAAAT ACATTGATCG CATCCGTGCC GATTATACCA AGGACCTCAA GAGCGAGGTC 
    ATGGCTGACA GGCAACGCGC AACGGCCATG TACCTCATTG ACAAGTTCGC TCTCAGAGCC 
    GGTAACGAAA AGGATACCGA GAACGAGGCC GAGACTGTCG GTTGCTGCTC TCTCAAGTAC 
    GAGCACGTCA CCCTCAGGGA ACCCAACACT GTCATTTTCG ACTTCTTGGG CAAGGACAGT 
    ATTCGTTTCT ACAACGAGTT CACGGTCGAT CGCCAGGTCT TCAAGAACCT CAAAATGTTC 
    AAGAAGCCTC CCAAGGAGGA CGGAGATGAC ATCTTTGACA GACTGACGAC AACCCAACTC 
    AACAAGCACT TGTCGAGCTA CATGCCTGGG CTCACGGCAA AGGTTTTCCG TACTTACAAC 
    GCATCGTACA CCATGTCCAA GCTGCTGCAG GAGCTCAAGG TGACGAATGC CACGGTACAT 
    GAAAAGGTCA AGCTCTACAA TGACTGCAAC CGCCAAGTTG CTATTCTTTG CAACCATAAG 
    CGTACTGTCG GTGCTAGCCA TGAGCAGCAA ATGAAGAAAC TTGTCGAGCA GATCAAGGGT 
    TTGCGATACC AGAAATGGCG GACAAAGATG ATGATGCTCG ACCTTGATCC CAAGCTCAAG 
    AAGAAGAAGG GTGCTGCCTA CTTTGAGCTT GATGAGGACT TGACAGAGGA GTGGGTCAAG 
    TCTCATCAGG AGTTCCTCGT TGAGGACCAG AAGGCCAAGA TCAACAAGAA GTTCGAAAAG 
    GACAATGAGA AGCGGATAGC TGATGGCGAG AAGCCTCTGC CTGCCAGCGA GCTCAAGGAG 
    CGACTCAAGG CGGCATCCGA GCTAGAAGCC AAGTTCAAGA AGGAGAACAA GACCAAGAAG 
    GTCGAGGCTG AAGGCAAGGG TCCTTCTGTC GAGAAGCTGG AGGCAGCTCT TGAGAAGCTT 
    GATCAACGTA TCAAGAACCT GGAGCTCCAG TCCGCGGATC GTGAAGGAAA CAAAGAGGTT 
    GCTCTTGGCA CTTCCAAAAT TAACTACATT GATCCCCGTC TTACCGTCGT CTTCTCGGCC 
    AAGTTTAATG TGCCTATCGA GAAGTTCTTC TCCAAGACGT TGCGCGAAAA GTTCAAGTGG 
    GCTATCGAGT CTGTCGGAAA CGACTCGACG TGGGAGTTCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011393241.1
    CDS728..3409
    Translation

    Target ORF information:

    RefSeq Version XM_011394939.1
    Organism Neurospora crassa OR74A
    Definition Neurospora crassa OR74A DNA topoisomerase I, variant (NCU09118), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_011394939.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGGACGCTG CTGATTCCAA GGTTCACATG GCTCCCGTTG GTGTCTCCAT CCGTAATGGC 
    CCCGTCGACC AGGACAGGAT GGACGTCGAC TCCTCAGTAA GCGGTCAGGC TAAGCGCAAA 
    TCGCGGACAT CCATTGATCA GTCCATCAAC TATAAGGATG ACTCCAGTGA TGACGAAGCA 
    CCCTTGGCGA AACGTTCAAA AACACAACAT AAGCAACCAG ACTCCGATAG CGACGACGAG 
    CCGATTGCGA AAAAAAATGG CGCGAGATTG CCTCCCTCCG CCAAGAATAC GGATATGGAC 
    GTAGATTCTG ATGACGACGA ACCTCTCGGA AAGATCGCCG CGAAGAAGGC CAATATCGAG 
    AAGGAAGCCG CCAAGGAAGC CAAGAGTATT CGTGCTGGTA CCAAGGCCAA GAAGCCTGCT 
    GCTAAGAAGG CCGTCAAGGA TGAATCCTCC GATGACGAAC CCCTTGCGAA ACCCACCAAG 
    CGACAGGTCA ACGGCACAGC TGCGAAGAAG ACGAATGGTG TGAAGAAGGA AGAGTCTGAC 
    TCGGATGTGC CCATCGCTAA GCGGGCGAAG AAGGCACCTG CCGCGAAGCC TGCTGCAAAG 
    CCTGCTGCAA AGCCGGCTGC AAAGGCTGCC ACGAAGGCCG CCCCGAAGGG CAAGGTTGCC 
    GCTGTCAAGA AGTCCGTCAA GGAGGAATCG AAGGACATCA CCGATGAGGA CGATGAGGAA 
    GAGGAGTATA AGTGGTGGGA GGAGTCGGCA AAGAAGGAAG ATGACAGTAT CAAGTGGACC 
    ACCTTGGAGC ACAACGGCGT CATCTTCCCC GAGGATTACC AGCGTTTGCC CAAGGACGTC 
    AAACTGCTCT ACGATGGAAC GCCGGTCGAA CTCGATATCG AGGCCGAGGA AGCTGCGACC 
    TTTTTCGGCT TCATGCTTAA CTCGACGGTG AATGTTGAGA ACCCAACTTT CCAGAAGAAC 
    TTCTTCAGGG ACTTCAAGGA GATCCTAGAT CGGACAGGTG GCGCCAAGGA CAAGAATGGC 
    AAAAAGGTTG ACATCAAGGA ATTCGAAAAA CTCGACTTCT CCCAGTTGTG GGCTTACAAA 
    GATGCTCAAA CGAAGGCTAA GAATGCCCGG TCGTCAGCAG AGAAGAAGGC CGAGAAAGCC 
    GAAAGGGACA AGAGGGACGC CCCCTATCTA TATTGCAAGT GGGATGGGCG AAAAGAAAAG 
    GTTGGTAACC CCAGAGTGGA AATTCCAGGA CTTTTCCGTG GTCGCGGTGA TCACCCCAAG 
    ACCGGGTGTA TCAAGAAGCG TGTTTTGCCA GAGCAAATTA CCATCAACAT CGGCAAAGAG 
    GCCAAGGTCC CAGAGCCGCC AGCTGGCCAC AAGTGGAAGG CCGTTCAACA TGACAACAAG 
    GCAACTTGGC TCGCGATGTG GCAAGAGAAC ATCAACGGAC ACTACAAGTA TGTGATGTTG 
    GCAGCAAACA GTACTGTGAA GGGCAAGGCC GATTTCAAGA AGTTCGAAAA GGCCCGTGAG 
    CTCAAGAAAT ACATTGATCG CATCCGTGCC GATTATACCA AGGACCTCAA GAGCGAGGTC 
    ATGGCTGACA GGCAACGCGC AACGGCCATG TACCTCATTG ACAAGTTCGC TCTCAGAGCC 
    GGTAACGAAA AGGATACCGA GAACGAGGCC GAGACTGTCG GTTGCTGCTC TCTCAAGTAC 
    GAGCACGTCA CCCTCAGGGA ACCCAACACT GTCATTTTCG ACTTCTTGGG CAAGGACAGT 
    ATTCGTTTCT ACAACGAGTT CACGGTCGAT CGCCAGGTCT TCAAGAACCT CAAAATGTTC 
    AAGAAGCCTC CCAAGGAGGA CGGAGATGAC ATCTTTGACA GACTGACGAC AACCCAACTC 
    AACAAGCACT TGTCGAGCTA CATGCCTGGG CTCACGGCAA AGGTTTTCCG TACTTACAAC 
    GCATCGTACA CCATGTCCAA GCTGCTGCAG GAGCTCAAGG TGACGAATGC CACGGTACAT 
    GAAAAGGTCA AGCTCTACAA TGACTGCAAC CGCCAAGTTG CTATTCTTTG CAACCATAAG 
    CGTACTGTCG GTGCTAGCCA TGAGCAGCAA ATGAAGAAAC TTGTCGAGCA GATCAAGGGT 
    TTGCGATACC AGAAATGGCG GACAAAGATG ATGATGCTCG ACCTTGATCC CAAGCTCAAG 
    AAGAAGAAGG GTGCTGCCTA CTTTGAGCTT GATGAGGACT TGACAGAGGA GTGGGTCAAG 
    TCTCATCAGG AGTTCCTCGT TGAGGACCAG AAGGCCAAGA TCAACAAGAA GTTCGAAAAG 
    GACAATGAGA AGCGGATAGC TGATGGCGAG AAGCCTCTGC CTGCCAGCGA GCTCAAGGAG 
    CGACTCAAGG CGGCATCCGA GCTAGAAGCC AAGTTCAAGA AGGAGAACAA GACCAAGAAG 
    GTCGAGGCTG AAGGCAAGGG TCCTTCTGTC GAGAAGCTGG AGGCAGCTCT TGAGAAGCTT 
    GATCAACGTA TCAAGAACCT GGAGCTCCAG TCCGCGGATC GTGAAGGAAA CAAAGAGGTT 
    GCTCTTGGCA CTTCCAAAAT TAACTACATT GATCCCCGTC TTACCGTCGT CTTCTCGGCC 
    AAGTTTAATG TGCCTATCGA GAAGTTCTTC TCCAAGACGT TGCGCGAAAA GTTCAAGTGG 
    GCTATCGAGT CTGTCGGAAA CGACTCGACG TGGGAGTTCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    NCU09118 ( XM_011394940.1 ) cDNA ORF clone, Neurospora crassa OR74A -> XP_011393242.1 Neurospora crassa OR74A DNA topoisomerase I (NCU09118), mRNA.
    Price & Availability↑
    CloneID ONf10612
    Clone ID Related Accession (Same CDS sequence) XM_011394940.1
    Accession Version XM_011394940.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2772bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-10
    Organism Neurospora crassa OR74A
    Product DNA topoisomerase I
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026501). 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGTCTTCGG GCTCTGAAGA CGACAAGCCG CTCGCCAAGG TCAACGGCCA CGCCTCGGCG 
    CCGACCATCT CCAAGGCCGA TGATCGTGCC ATGGACGCTG CTGATTCCAA GGTTCACATG 
    GCTCCCGTTG GTGTCTCCAT CCGTAATGGC CCCGTCGACC AGGACAGGAT GGACGTCGAC 
    TCCTCAGTAA GCGGTCAGGC TAAGCGCAAA TCGCGGACAT CCATTGATCA GTCCATCAAC 
    TATAAGGATG ACTCCAGTGA TGACGAAGCA CCCTTGGCGA AACGTTCAAA AACACAACAT 
    AAGCAACCAG ACTCCGATAG CGACGACGAG CCGATTGCGA AAAAAAATGG CGCGAGATTG 
    CCTCCCTCCG CCAAGAATAC GGATATGGAC GTAGATTCTG ATGACGACGA ACCTCTCGGA 
    AAGATCGCCG CGAAGAAGGC CAATATCGAG AAGGAAGCCG CCAAGGAAGC CAAGAGTATT 
    CGTGCTGGTA CCAAGGCCAA GAAGCCTGCT GCTAAGAAGG CCGTCAAGGA TGAATCCTCC 
    GATGACGAAC CCCTTGCGAA ACCCACCAAG CGACAGGTCA ACGGCACAGC TGCGAAGAAG 
    ACGAATGGTG TGAAGAAGGA AGAGTCTGAC TCGGATGTGC CCATCGCTAA GCGGGCGAAG 
    AAGGCACCTG CCGCGAAGCC TGCTGCAAAG CCTGCTGCAA AGCCGGCTGC AAAGGCTGCC 
    ACGAAGGCCG CCCCGAAGGG CAAGGTTGCC GCTGTCAAGA AGTCCGTCAA GGAGGAATCG 
    AAGGACATCA CCGATGAGGA CGATGAGGAA GAGGAGTATA AGTGGTGGGA GGAGTCGGCA 
    AAGAAGGAAG ATGACAGTAT CAAGTGGACC ACCTTGGAGC ACAACGGCGT CATCTTCCCC 
    GAGGATTACC AGCGTTTGCC CAAGGACGTC AAACTGCTCT ACGATGGAAC GCCGGTCGAA 
    CTCGATATCG AGGCCGAGGA AGCTGCGACC TTTTTCGGCT TCATGCTTAA CTCGACGGTG 
    AATGTTGAGA ACCCAACTTT CCAGAAGAAC TTCTTCAGGG ACTTCAAGGA GATCCTAGAT 
    CGGACAGGTG GCGCCAAGGA CAAGAATGGC AAAAAGGTTG ACATCAAGGA ATTCGAAAAA 
    CTCGACTTCT CCCAGTTGTG GGCTTACAAA GATGCTCAAA CGAAGGCTAA GAATGCCCGG 
    TCGTCAGCAG AGAAGAAGGC CGAGAAAGCC GAAAGGGACA AGAGGGACGC CCCCTATCTA 
    TATTGCAAGT GGGATGGGCG AAAAGAAAAG GTTGGTAACC CCAGAGTGGA AATTCCAGGA 
    CTTTTCCGTG GTCGCGGTGA TCACCCCAAG ACCGGGTGTA TCAAGAAGCG TGTTTTGCCA 
    GAGCAAATTA CCATCAACAT CGGCAAAGAG GCCAAGGTCC CAGAGCCGCC AGCTGGCCAC 
    AAGTGGAAGG CCGTTCAACA TGACAACAAG GCAACTTGGC TCGCGATGTG GCAAGAGAAC 
    ATCAACGGAC ACTACAAGTA TGTGATGTTG GCAGCAAACA GTACTGTGAA GGGCAAGGCC 
    GATTTCAAGA AGTTCGAAAA GGCCCGTGAG CTCAAGAAAT ACATTGATCG CATCCGTGCC 
    GATTATACCA AGGACCTCAA GAGCGAGGTC ATGGCTGACA GGCAACGCGC AACGGCCATG 
    TACCTCATTG ACAAGTTCGC TCTCAGAGCC GGTAACGAAA AGGATACCGA GAACGAGGCC 
    GAGACTGTCG GTTGCTGCTC TCTCAAGTAC GAGCACGTCA CCCTCAGGGA ACCCAACACT 
    GTCATTTTCG ACTTCTTGGG CAAGGACAGT ATTCGTTTCT ACAACGAGTT CACGGTCGAT 
    CGCCAGGTCT TCAAGAACCT CAAAATGTTC AAGAAGCCTC CCAAGGAGGA CGGAGATGAC 
    ATCTTTGACA GACTGACGAC AACCCAACTC AACAAGCACT TGTCGAGCTA CATGCCTGGG 
    CTCACGGCAA AGGTTTTCCG TACTTACAAC GCATCGTACA CCATGTCCAA GCTGCTGCAG 
    GAGCTCAAGG TGACGAATGC CACGGTACAT GAAAAGGTCA AGCTCTACAA TGACTGCAAC 
    CGCCAAGTTG CTATTCTTTG CAACCATAAG CGTACTGTCG GTGCTAGCCA TGAGCAGCAA 
    ATGAAGAAAC TTGTCGAGCA GATCAAGGGT TTGCGATACC AGAAATGGCG GACAAAGATG 
    ATGATGCTCG ACCTTGATCC CAAGCTCAAG AAGAAGAAGG GTGCTGCCTA CTTTGAGCTT 
    GATGAGGACT TGACAGAGGA GTGGGTCAAG TCTCATCAGG AGTTCCTCGT TGAGGACCAG 
    AAGGCCAAGA TCAACAAGAA GTTCGAAAAG GACAATGAGA AGCGGATAGC TGATGGCGAG 
    AAGCCTCTGC CTGCCAGCGA GCTCAAGGAG CGACTCAAGG CGGCATCCGA GCTAGAAGCC 
    AAGTTCAAGA AGGAGAACAA GACCAAGAAG GTCGAGGCTG AAGGCAAGGG TCCTTCTGTC 
    GAGAAGCTGG AGGCAGCTCT TGAGAAGCTT GATCAACGTA TCAAGAACCT GGAGCTCCAG 
    TCCGCGGATC GTGAAGGAAA CAAAGAGGTT GCTCTTGGCA CTTCCAAAAT TAACTACATT 
    GATCCCCGTC TTACCGTCGT CTTCTCGGCC AAGTTTAATG TGCCTATCGA GAAGTTCTTC 
    TCCAAGACGT TGCGCGAAAA GTTCAAGTGG GCTATCGAGT CTGTCGGAAA CGACTCGACG 
    TGGGAGTTCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011393242.1
    CDS734..3505
    Translation

    Target ORF information:

    RefSeq Version XM_011394940.1
    Organism Neurospora crassa OR74A
    Definition Neurospora crassa OR74A DNA topoisomerase I (NCU09118), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_011394940.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGTCTTCGG GCTCTGAAGA CGACAAGCCG CTCGCCAAGG TCAACGGCCA CGCCTCGGCG 
    CCGACCATCT CCAAGGCCGA TGATCGTGCC ATGGACGCTG CTGATTCCAA GGTTCACATG 
    GCTCCCGTTG GTGTCTCCAT CCGTAATGGC CCCGTCGACC AGGACAGGAT GGACGTCGAC 
    TCCTCAGTAA GCGGTCAGGC TAAGCGCAAA TCGCGGACAT CCATTGATCA GTCCATCAAC 
    TATAAGGATG ACTCCAGTGA TGACGAAGCA CCCTTGGCGA AACGTTCAAA AACACAACAT 
    AAGCAACCAG ACTCCGATAG CGACGACGAG CCGATTGCGA AAAAAAATGG CGCGAGATTG 
    CCTCCCTCCG CCAAGAATAC GGATATGGAC GTAGATTCTG ATGACGACGA ACCTCTCGGA 
    AAGATCGCCG CGAAGAAGGC CAATATCGAG AAGGAAGCCG CCAAGGAAGC CAAGAGTATT 
    CGTGCTGGTA CCAAGGCCAA GAAGCCTGCT GCTAAGAAGG CCGTCAAGGA TGAATCCTCC 
    GATGACGAAC CCCTTGCGAA ACCCACCAAG CGACAGGTCA ACGGCACAGC TGCGAAGAAG 
    ACGAATGGTG TGAAGAAGGA AGAGTCTGAC TCGGATGTGC CCATCGCTAA GCGGGCGAAG 
    AAGGCACCTG CCGCGAAGCC TGCTGCAAAG CCTGCTGCAA AGCCGGCTGC AAAGGCTGCC 
    ACGAAGGCCG CCCCGAAGGG CAAGGTTGCC GCTGTCAAGA AGTCCGTCAA GGAGGAATCG 
    AAGGACATCA CCGATGAGGA CGATGAGGAA GAGGAGTATA AGTGGTGGGA GGAGTCGGCA 
    AAGAAGGAAG ATGACAGTAT CAAGTGGACC ACCTTGGAGC ACAACGGCGT CATCTTCCCC 
    GAGGATTACC AGCGTTTGCC CAAGGACGTC AAACTGCTCT ACGATGGAAC GCCGGTCGAA 
    CTCGATATCG AGGCCGAGGA AGCTGCGACC TTTTTCGGCT TCATGCTTAA CTCGACGGTG 
    AATGTTGAGA ACCCAACTTT CCAGAAGAAC TTCTTCAGGG ACTTCAAGGA GATCCTAGAT 
    CGGACAGGTG GCGCCAAGGA CAAGAATGGC AAAAAGGTTG ACATCAAGGA ATTCGAAAAA 
    CTCGACTTCT CCCAGTTGTG GGCTTACAAA GATGCTCAAA CGAAGGCTAA GAATGCCCGG 
    TCGTCAGCAG AGAAGAAGGC CGAGAAAGCC GAAAGGGACA AGAGGGACGC CCCCTATCTA 
    TATTGCAAGT GGGATGGGCG AAAAGAAAAG GTTGGTAACC CCAGAGTGGA AATTCCAGGA 
    CTTTTCCGTG GTCGCGGTGA TCACCCCAAG ACCGGGTGTA TCAAGAAGCG TGTTTTGCCA 
    GAGCAAATTA CCATCAACAT CGGCAAAGAG GCCAAGGTCC CAGAGCCGCC AGCTGGCCAC 
    AAGTGGAAGG CCGTTCAACA TGACAACAAG GCAACTTGGC TCGCGATGTG GCAAGAGAAC 
    ATCAACGGAC ACTACAAGTA TGTGATGTTG GCAGCAAACA GTACTGTGAA GGGCAAGGCC 
    GATTTCAAGA AGTTCGAAAA GGCCCGTGAG CTCAAGAAAT ACATTGATCG CATCCGTGCC 
    GATTATACCA AGGACCTCAA GAGCGAGGTC ATGGCTGACA GGCAACGCGC AACGGCCATG 
    TACCTCATTG ACAAGTTCGC TCTCAGAGCC GGTAACGAAA AGGATACCGA GAACGAGGCC 
    GAGACTGTCG GTTGCTGCTC TCTCAAGTAC GAGCACGTCA CCCTCAGGGA ACCCAACACT 
    GTCATTTTCG ACTTCTTGGG CAAGGACAGT ATTCGTTTCT ACAACGAGTT CACGGTCGAT 
    CGCCAGGTCT TCAAGAACCT CAAAATGTTC AAGAAGCCTC CCAAGGAGGA CGGAGATGAC 
    ATCTTTGACA GACTGACGAC AACCCAACTC AACAAGCACT TGTCGAGCTA CATGCCTGGG 
    CTCACGGCAA AGGTTTTCCG TACTTACAAC GCATCGTACA CCATGTCCAA GCTGCTGCAG 
    GAGCTCAAGG TGACGAATGC CACGGTACAT GAAAAGGTCA AGCTCTACAA TGACTGCAAC 
    CGCCAAGTTG CTATTCTTTG CAACCATAAG CGTACTGTCG GTGCTAGCCA TGAGCAGCAA 
    ATGAAGAAAC TTGTCGAGCA GATCAAGGGT TTGCGATACC AGAAATGGCG GACAAAGATG 
    ATGATGCTCG ACCTTGATCC CAAGCTCAAG AAGAAGAAGG GTGCTGCCTA CTTTGAGCTT 
    GATGAGGACT TGACAGAGGA GTGGGTCAAG TCTCATCAGG AGTTCCTCGT TGAGGACCAG 
    AAGGCCAAGA TCAACAAGAA GTTCGAAAAG GACAATGAGA AGCGGATAGC TGATGGCGAG 
    AAGCCTCTGC CTGCCAGCGA GCTCAAGGAG CGACTCAAGG CGGCATCCGA GCTAGAAGCC 
    AAGTTCAAGA AGGAGAACAA GACCAAGAAG GTCGAGGCTG AAGGCAAGGG TCCTTCTGTC 
    GAGAAGCTGG AGGCAGCTCT TGAGAAGCTT GATCAACGTA TCAAGAACCT GGAGCTCCAG 
    TCCGCGGATC GTGAAGGAAA CAAAGAGGTT GCTCTTGGCA CTTCCAAAAT TAACTACATT 
    GATCCCCGTC TTACCGTCGT CTTCTCGGCC AAGTTTAATG TGCCTATCGA GAAGTTCTTC 
    TCCAAGACGT TGCGCGAAAA GTTCAAGTGG GCTATCGAGT CTGTCGGAAA CGACTCGACG 
    TGGGAGTTCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The genome sequence of the filamentous fungus Neurospora crassa.
    Nature422(6934)859-68(2003 Apr)
    Galagan JE,Calvo SE,Borkovich KA,Selker EU,Read ND,Jaffe D,FitzHugh W,Ma LJ,Smirnov S,Purcell S,Rehman B,Elkins T,Engels R,Wang S,Nielsen CB,Butler J,Endrizzi M,Qui D,Ianakiev P,Bell-Pedersen D,Nelson MA,Werner-Washburne M,Selitrennikoff CP,Kinsey JA,Braun EL,Zelter A,Schulte U,Kothe GO,Jedd G,Mewes W,Staben C,Marcotte E,Greenberg D,Roy A,Foley K,Naylor J,Stange-Thomann N,Barrett R,Gnerre S,Kamal M,Kamvysselis M,Mauceli E,Bielke C,Rudd S,Frishman D,Krystofova S,Rasmussen C,Metzenberg RL,Perkins DD,Kroken S,Cogoni C,Macino G,Catcheside D,Li W,Pratt RJ,Osmani SA,DeSouza CP,Glass L,Orbach MJ,Berglund JA,Voelker R,Yarden O,Plamann M,Seiler S,Dunlap J,Radford A,Aramayo R,Natvig DO,Alex LA,Mannhaupt G,Ebbole DJ,Freitag M,Paulsen I,Sachs MS,Lander ES,Nusbaum C,Birren B


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