NCU00302 cDNA ORF clone, Neurospora crassa OR74A

  • Gene

  • Clones

  • gRNAs

The following NCU00302 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NCU00302 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ONf10527 XM_952680.3
Latest version!
Neurospora crassa OR74A 3-ketodihydrosphingosine reductase tsc-10 (NCU00302), mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $258.30
$369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ONf10527
    Clone ID Related Accession (Same CDS sequence) XM_952680.3
    Accession Version XM_952680.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1089bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-10
    Organism Neurospora crassa OR74A
    Product 3-ketodihydrosphingosine reductase tsc-10
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026503). On Feb 23, 2015 this sequence version replaced XM_952680.2.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    ATGAACCATT ATCTCTTCTC AGAGTCGCTG ATGGAACAAT TGAAAACCCT CAGCACCTCC 
    TGGTCTCTCC CAGTTGCAGC CGTCGTCGCG GCCATTGGCA TATTTGCTAC AATGGGTCTC
    TTCAGCTCCA AAAACCACAT GCCAGTCGAG GGCCGGACCG TCCTCCTAAC CGGCGCCTCC
    GAAGGCATGG GCCGCTCAGC GGCCATCCAA CTTTCGCAAA AGGGCGCCAA TGTCATCCTC
    GTCTCGCGCA ACGTCGGGCG CCTTGAAGAA GCCCTCGTCG ACGTCCGCGC CGCCGCCAAG
    AACCCGTCCA CACAACGCTT CACTTACATC TCAGCCGACG TTTCCGAGCA CGACTACGCC
    GCCGCGGTCC TCGCCGAAGC TATCGCCTGG AATGGCGGGC GCTCCCCGGA CATCGTCTGG
    TGCGTGGCCG GCATGTCGAC GCCTCTGCTG TGGACCGACG ATGGCAGCAT GGCGGCCGCA
    CGCCGCAACA TGGACGTCAA CTACTTTGGG TCGGCGGAGA TGTCGCGGGC GATTTTGAGG
    GAGTGGCTGG CACCCGAAAA CAGCACGGGA CCCAACGGAG AGCCGAAGCA CTTGGTATTT
    ACCGCGTCAA TGTTGGCGCT TTTTGCGATT TTGGGCTATG GCCCGTACAC CCCAACCAAA
    TGGGCGCTGA GAGGACTGGC GGATACCCTG GCGATGGAGG TCAATTACTA CCCAGATAAC
    CCGGTCAAGG TGCACATTGT GTATCCCGGC ACGATTGTAT CACCGGGCTA CGAGAGGGAA
    AATCAGACGA AGCCGGACAT CACGGTGGAG CTGGAAAAGG ACGAGCCAGC GGAAAGCCCG
    GATACGGTAG CAAGGAGGGC GATTGCCGGG CTGGAGGCTG GCAAGTATTT TGTCGATGTG
    TCATTCCTGG GAAGGCTGAT GCAGTGCGGG ATCATGGGCG GGTCGCCGAG GAACAACTGG
    GTGCTGGACA CGTTGATGGG GTGGCTCATA CCGATCATCT ACTTCTTCGT GTTGAGGGGG
    ATGAACTCAA CGATTGTGAA GTGGGCGAGG GAAAAGGGGC ATCCTTTTAC GCATCCCAAG
    AAGAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_957773.3
    CDS146..1234
    Translation

    Target ORF information:

    RefSeq Version XM_952680.3
    Organism Neurospora crassa OR74A
    Definition Neurospora crassa OR74A 3-ketodihydrosphingosine reductase tsc-10 (NCU00302), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_952680.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    ATGAACCATT ATCTCTTCTC AGAGTCGCTG ATGGAACAAT TGAAAACCCT CAGCACCTCC 
    TGGTCTCTCC CAGTTGCAGC CGTCGTCGCG GCCATTGGCA TATTTGCTAC AATGGGTCTC
    TTCAGCTCCA AAAACCACAT GCCAGTCGAG GGCCGGACCG TCCTCCTAAC CGGCGCCTCC
    GAAGGCATGG GCCGCTCAGC GGCCATCCAA CTTTCGCAAA AGGGCGCCAA TGTCATCCTC
    GTCTCGCGCA ACGTCGGGCG CCTTGAAGAA GCCCTCGTCG ACGTCCGCGC CGCCGCCAAG
    AACCCGTCCA CACAACGCTT CACTTACATC TCAGCCGACG TTTCCGAGCA CGACTACGCC
    GCCGCGGTCC TCGCCGAAGC TATCGCCTGG AATGGCGGGC GCTCCCCGGA CATCGTCTGG
    TGCGTGGCCG GCATGTCGAC GCCTCTGCTG TGGACCGACG ATGGCAGCAT GGCGGCCGCA
    CGCCGCAACA TGGACGTCAA CTACTTTGGG TCGGCGGAGA TGTCGCGGGC GATTTTGAGG
    GAGTGGCTGG CACCCGAAAA CAGCACGGGA CCCAACGGAG AGCCGAAGCA CTTGGTATTT
    ACCGCGTCAA TGTTGGCGCT TTTTGCGATT TTGGGCTATG GCCCGTACAC CCCAACCAAA
    TGGGCGCTGA GAGGACTGGC GGATACCCTG GCGATGGAGG TCAATTACTA CCCAGATAAC
    CCGGTCAAGG TGCACATTGT GTATCCCGGC ACGATTGTAT CACCGGGCTA CGAGAGGGAA
    AATCAGACGA AGCCGGACAT CACGGTGGAG CTGGAAAAGG ACGAGCCAGC GGAAAGCCCG
    GATACGGTAG CAAGGAGGGC GATTGCCGGG CTGGAGGCTG GCAAGTATTT TGTCGATGTG
    TCATTCCTGG GAAGGCTGAT GCAGTGCGGG ATCATGGGCG GGTCGCCGAG GAACAACTGG
    GTGCTGGACA CGTTGATGGG GTGGCTCATA CCGATCATCT ACTTCTTCGT GTTGAGGGGG
    ATGAACTCAA CGATTGTGAA GTGGGCGAGG GAAAAGGGGC ATCCTTTTAC GCATCCCAAG
    AAGAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The genome sequence of the filamentous fungus Neurospora crassa.
    Nature422(6934)859-68(2003 Apr)
    Galagan JE,Calvo SE,Borkovich KA,Selker EU,Read ND,Jaffe D,FitzHugh W,Ma LJ,Smirnov S,Purcell S,Rehman B,Elkins T,Engels R,Wang S,Nielsen CB,Butler J,Endrizzi M,Qui D,Ianakiev P,Bell-Pedersen D,Nelson MA,Werner-Washburne M,Selitrennikoff CP,Kinsey JA,Braun EL,Zelter A,Schulte U,Kothe GO,Jedd G,Mewes W,Staben C,Marcotte E,Greenberg D,Roy A,Foley K,Naylor J,Stange-Thomann N,Barrett R,Gnerre S,Kamal M,Kamvysselis M,Mauceli E,Bielke C,Rudd S,Frishman D,Krystofova S,Rasmussen C,Metzenberg RL,Perkins DD,Kroken S,Cogoni C,Macino G,Catcheside D,Li W,Pratt RJ,Osmani SA,DeSouza CP,Glass L,Orbach MJ,Berglund JA,Voelker R,Yarden O,Plamann M,Seiler S,Dunlap J,Radford A,Aramayo R,Natvig DO,Alex LA,Mannhaupt G,Ebbole DJ,Freitag M,Paulsen I,Sachs MS,Lander ES,Nusbaum C,Birren B