ORF » Species Summary » Neurospora crassa OR74A » NCU00403 cDNA ORF clone

NCU00403 cDNA ORF clone, Neurospora crassa OR74A

The following NCU00403 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NCU00403 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ONf10344 XM_011395531.1
Latest version!		 Neurospora crassa OR74A dTDP-D-glucose 4,6-dehydratase (NCU00403), mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
ONf10345 XM_011395532.1
Latest version!		 Neurospora crassa OR74A dTDP-D-glucose 4,6-dehydratase, variant 1 (NCU00403), mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
ONf10345 XM_011395533.1
Latest version!		 Neurospora crassa OR74A dTDP-D-glucose 4,6-dehydratase, variant 2 (NCU00403), mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    NCU00403 ( XM_011395531.1 ) cDNA ORF clone, Neurospora crassa OR74A -> XP_011393833.1 Neurospora crassa OR74A dTDP-D-glucose 4,6-dehydratase (NCU00403), mRNA.
    Price & Availability↑
    CloneID ONf10344
    Clone ID Related Accession (Same CDS sequence) XM_011395531.1
    Accession Version XM_011395531.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1947bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-10
    Organism Neurospora crassa OR74A
    Product dTDP-D-glucose 4,6-dehydratase
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026503). 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGTTTCACT GCACCTTTTA TTCATCGGGC GTACGCATAC GCCTCTACGC GAACTCCCGG 
    GTGGCTCATA AGGAAGGGTG CACCGCATCC AGCCACTTTG CCATCAGACC TTGTTTCTTC 
    GGGTATTCAA GAGGCGGCCA GAACCAAATA ATGACTTTCG CAACACAAGA GCCAACCAGC 
    CGGTACAACA GCGCAGTCAC GCCTATTTCA CCCAGCTCCC AACTGGAGAG CCCTTTCGAG 
    GTCAACGAAA TTTGGCGTGA TGCTCCTGTA TTCACCGGCA CTACAAAGTT TGATCCTCTG 
    CCTGATGTAA AGAACATCAT GGTAACAGGA GGCGCTGGTT TCATAGCATG TTGGGTTGTC 
    AGACACTTGG TATTGACGTA TCCAGAAGCC TACAATGTCG TCTCATTCGA CAAGTTAGAC 
    TACTGCGCAT CGCTCAACAA CACCAGGGTA CTAGAACATG AGCCGAACTT CTCGTTTTAC 
    AGAGGCGACA TTACGAATCC TTCCGAGGTC ATGGACTGTC TGGAGCGATA CAATATCGAC 
    ACCATCTTTC ACTTTGCCGC GCAATCACAT GTCGATTTGA GTTTTGGAAA TCCGTTCGGT 
    TTCACTCATA CCAATGTTTA CGGTACCCAT GTTCTGCTCG AGAGCGCTCG AAAAGCGGGC 
    ATCAAGCGCT TCATACACGT GTCTACCGAT GAAGTATATG GCGAGGTAAA GGATGATGAA 
    GATGATCTTC TGGAGACGAG CATTCTGGCA CCCACTAACC CCTATGCGGC CAGCAAAGCT 
    GCTGCCGAGA TGCTGGTGAA CTCTTACAAG AAGAGCTTTA AATTACCCAT CATTATAGTG 
    AGGAGCAACA ATGTCTATGG CCCTCACCAG TACCCGGAGA AAATCATCCC AAAGTTCACT 
    TGCCTACTCG CCCGAGGAGA GCCCGTCGTC CTTCACGGAG ACGGCTCGCC CACTCGTCGT 
    TATCTTTTTG CAGGCGACGC CGCCGACGCG TTCGATACCA TTCTGCACAG AGGTCAGCTA 
    GGGGAGATCT ATAACGTTGG TTCTTACGAT GAAATCAGCA ACTTGTCGCT TTGCCACAAA 
    CTCCTAACCG AGATGGAGAT CATCCCCTGT CGGTCTCCGA CATCATCTCC TCCTTGCAAC 
    TATACCACAT CCCCGCCCTC CTCGTCGCCT CTGGGGGCAG AACAAGAAGA GATCCATCGT 
    TGGGTCAAGT ACACTCACGA CCGACCTTTC AACGATCACC GGTATGCCGT TGACGGTACG 
    AAACTGCGAA AACTAGGTTG GGAGCCGAAG ACAACTTTTG AAGAGGGGCT CAGGATCACT 
    GTGGACTGGT ACAGACGGTT TGGGGAAAGG TGGTGGGATG ATATTACCAA CGTATTGAGG 
    CCCTTCCCTA TAACAGCGGG GGGCGAAGAC GGGGTAATGG ATCAAGAGGT GGCCGAGATG 
    TGTGACAAGC CCGTCACTTT GGAATCAAGA GAGAATACGG GGGTCAGAAA GTCATCCGAT 
    ATACTGAAGG AAGCATCGGG TTCGTTCAGG TCACGCCACC AACAATATCA GCATCAACAT 
    CATCACAAAC ACCGCACAAG AAAATATCTT CACCACACAA GGGAAACGTC GCGAGACAAC 
    ACGGATTATT ATTCTAGCAG GATACCAAGA AGCTGCGCTC TAGCTTCTCC ACGCCCACGA 
    GAGCTCGCCT CCAGCACCGG CCTTGTAAGC CCAGGGATCA CATTAGATGA GGACGATGGT 
    TTCGACGATG AGGATACGGC ATATAACCCA CTAACGATCG TTTACGACGA CGAGGCACGA 
    TCACGAAAGA AGCGCAAGCT TTCAATCGTG GTCGATGATA TCACCAACGA TAGCCACCGC 
    TTCAACAAGA AGGCCCGGGG AGATATTACT CAAGAAGGGG GTCGCTGTGC TAAACTGTCG 
    TGGACAGGCT TGACAAGAAC GTTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011393833.1
    CDS423..2369
    Translation

    Target ORF information:

    RefSeq Version XM_011395531.1
    Organism Neurospora crassa OR74A
    Definition Neurospora crassa OR74A dTDP-D-glucose 4,6-dehydratase (NCU00403), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_011395531.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGTTTCACT GCACCTTTTA TTCATCGGGC GTACGCATAC GCCTCTACGC GAACTCCCGG 
    GTGGCTCATA AGGAAGGGTG CACCGCATCC AGCCACTTTG CCATCAGACC TTGTTTCTTC 
    GGGTATTCAA GAGGCGGCCA GAACCAAATA ATGACTTTCG CAACACAAGA GCCAACCAGC 
    CGGTACAACA GCGCAGTCAC GCCTATTTCA CCCAGCTCCC AACTGGAGAG CCCTTTCGAG 
    GTCAACGAAA TTTGGCGTGA TGCTCCTGTA TTCACCGGCA CTACAAAGTT TGATCCTCTG 
    CCTGATGTAA AGAACATCAT GGTAACAGGA GGCGCTGGTT TCATAGCATG TTGGGTTGTC 
    AGACACTTGG TATTGACGTA TCCAGAAGCC TACAATGTCG TCTCATTCGA CAAGTTAGAC 
    TACTGCGCAT CGCTCAACAA CACCAGGGTA CTAGAACATG AGCCGAACTT CTCGTTTTAC 
    AGAGGCGACA TTACGAATCC TTCCGAGGTC ATGGACTGTC TGGAGCGATA CAATATCGAC 
    ACCATCTTTC ACTTTGCCGC GCAATCACAT GTCGATTTGA GTTTTGGAAA TCCGTTCGGT 
    TTCACTCATA CCAATGTTTA CGGTACCCAT GTTCTGCTCG AGAGCGCTCG AAAAGCGGGC 
    ATCAAGCGCT TCATACACGT GTCTACCGAT GAAGTATATG GCGAGGTAAA GGATGATGAA 
    GATGATCTTC TGGAGACGAG CATTCTGGCA CCCACTAACC CCTATGCGGC CAGCAAAGCT 
    GCTGCCGAGA TGCTGGTGAA CTCTTACAAG AAGAGCTTTA AATTACCCAT CATTATAGTG 
    AGGAGCAACA ATGTCTATGG CCCTCACCAG TACCCGGAGA AAATCATCCC AAAGTTCACT 
    TGCCTACTCG CCCGAGGAGA GCCCGTCGTC CTTCACGGAG ACGGCTCGCC CACTCGTCGT 
    TATCTTTTTG CAGGCGACGC CGCCGACGCG TTCGATACCA TTCTGCACAG AGGTCAGCTA 
    GGGGAGATCT ATAACGTTGG TTCTTACGAT GAAATCAGCA ACTTGTCGCT TTGCCACAAA 
    CTCCTAACCG AGATGGAGAT CATCCCCTGT CGGTCTCCGA CATCATCTCC TCCTTGCAAC 
    TATACCACAT CCCCGCCCTC CTCGTCGCCT CTGGGGGCAG AACAAGAAGA GATCCATCGT 
    TGGGTCAAGT ACACTCACGA CCGACCTTTC AACGATCACC GGTATGCCGT TGACGGTACG 
    AAACTGCGAA AACTAGGTTG GGAGCCGAAG ACAACTTTTG AAGAGGGGCT CAGGATCACT 
    GTGGACTGGT ACAGACGGTT TGGGGAAAGG TGGTGGGATG ATATTACCAA CGTATTGAGG 
    CCCTTCCCTA TAACAGCGGG GGGCGAAGAC GGGGTAATGG ATCAAGAGGT GGCCGAGATG 
    TGTGACAAGC CCGTCACTTT GGAATCAAGA GAGAATACGG GGGTCAGAAA GTCATCCGAT 
    ATACTGAAGG AAGCATCGGG TTCGTTCAGG TCACGCCACC AACAATATCA GCATCAACAT 
    CATCACAAAC ACCGCACAAG AAAATATCTT CACCACACAA GGGAAACGTC GCGAGACAAC 
    ACGGATTATT ATTCTAGCAG GATACCAAGA AGCTGCGCTC TAGCTTCTCC ACGCCCACGA 
    GAGCTCGCCT CCAGCACCGG CCTTGTAAGC CCAGGGATCA CATTAGATGA GGACGATGGT 
    TTCGACGATG AGGATACGGC ATATAACCCA CTAACGATCG TTTACGACGA CGAGGCACGA 
    TCACGAAAGA AGCGCAAGCT TTCAATCGTG GTCGATGATA TCACCAACGA TAGCCACCGC 
    TTCAACAAGA AGGCCCGGGG AGATATTACT CAAGAAGGGG GTCGCTGTGC TAAACTGTCG 
    TGGACAGGCT TGACAAGAAC GTTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    NCU00403 ( XM_011395533.1 ) cDNA ORF clone, Neurospora crassa OR74A -> XP_011393835.1 Neurospora crassa OR74A dTDP-D-glucose 4,6-dehydratase, variant 2 (NCU00403), mRNA.
    Price & Availability↑
    CloneID ONf10345
    Clone ID Related Accession (Same CDS sequence) XM_011395533.1 , XM_011395532.1
    Accession Version XM_011395533.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1797bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-10
    Organism Neurospora crassa OR74A
    Product dTDP-D-glucose 4,6-dehydratase, variant 2
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026503). 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGACTTTCG CAACACAAGA GCCAACCAGC CGGTACAACA GCGCAGTCAC GCCTATTTCA 
    CCCAGCTCCC AACTGGAGAG CCCTTTCGAG GTCAACGAAA TTTGGCGTGA TGCTCCTGTA 
    TTCACCGGCA CTACAAAGTT TGATCCTCTG CCTGATGTAA AGAACATCAT GGTAACAGGA 
    GGCGCTGGTT TCATAGCATG TTGGGTTGTC AGACACTTGG TATTGACGTA TCCAGAAGCC 
    TACAATGTCG TCTCATTCGA CAAGTTAGAC TACTGCGCAT CGCTCAACAA CACCAGGGTA 
    CTAGAACATG AGCCGAACTT CTCGTTTTAC AGAGGCGACA TTACGAATCC TTCCGAGGTC 
    ATGGACTGTC TGGAGCGATA CAATATCGAC ACCATCTTTC ACTTTGCCGC GCAATCACAT 
    GTCGATTTGA GTTTTGGAAA TCCGTTCGGT TTCACTCATA CCAATGTTTA CGGTACCCAT 
    GTTCTGCTCG AGAGCGCTCG AAAAGCGGGC ATCAAGCGCT TCATACACGT GTCTACCGAT 
    GAAGTATATG GCGAGGTAAA GGATGATGAA GATGATCTTC TGGAGACGAG CATTCTGGCA 
    CCCACTAACC CCTATGCGGC CAGCAAAGCT GCTGCCGAGA TGCTGGTGAA CTCTTACAAG 
    AAGAGCTTTA AATTACCCAT CATTATAGTG AGGAGCAACA ATGTCTATGG CCCTCACCAG 
    TACCCGGAGA AAATCATCCC AAAGTTCACT TGCCTACTCG CCCGAGGAGA GCCCGTCGTC 
    CTTCACGGAG ACGGCTCGCC CACTCGTCGT TATCTTTTTG CAGGCGACGC CGCCGACGCG 
    TTCGATACCA TTCTGCACAG AGGTCAGCTA GGGGAGATCT ATAACGTTGG TTCTTACGAT 
    GAAATCAGCA ACTTGTCGCT TTGCCACAAA CTCCTAACCG AGATGGAGAT CATCCCCTGT 
    CGGTCTCCGA CATCATCTCC TCCTTGCAAC TATACCACAT CCCCGCCCTC CTCGTCGCCT 
    CTGGGGGCAG AACAAGAAGA GATCCATCGT TGGGTCAAGT ACACTCACGA CCGACCTTTC 
    AACGATCACC GGTATGCCGT TGACGGTACG AAACTGCGAA AACTAGGTTG GGAGCCGAAG 
    ACAACTTTTG AAGAGGGGCT CAGGATCACT GTGGACTGGT ACAGACGGTT TGGGGAAAGG 
    TGGTGGGATG ATATTACCAA CGTATTGAGG CCCTTCCCTA TAACAGCGGG GGGCGAAGAC 
    GGGGTAATGG ATCAAGAGGT GGCCGAGATG TGTGACAAGC CCGTCACTTT GGAATCAAGA 
    GAGAATACGG GGGTCAGAAA GTCATCCGAT ATACTGAAGG AAGCATCGGG TTCGTTCAGG 
    TCACGCCACC AACAATATCA GCATCAACAT CATCACAAAC ACCGCACAAG AAAATATCTT 
    CACCACACAA GGGAAACGTC GCGAGACAAC ACGGATTATT ATTCTAGCAG GATACCAAGA 
    AGCTGCGCTC TAGCTTCTCC ACGCCCACGA GAGCTCGCCT CCAGCACCGG CCTTGTAAGC 
    CCAGGGATCA CATTAGATGA GGACGATGGT TTCGACGATG AGGATACGGC ATATAACCCA 
    CTAACGATCG TTTACGACGA CGAGGCACGA TCACGAAAGA AGCGCAAGCT TTCAATCGTG 
    GTCGATGATA TCACCAACGA TAGCCACCGC TTCAACAAGA AGGCCCGGGG AGATATTACT 
    CAAGAAGGGG GTCGCTGTGC TAAACTGTCG TGGACAGGCT TGACAAGAAC GTTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011393835.1
    CDS722..2518
    Translation

    Target ORF information:

    RefSeq Version XM_011395533.1
    Organism Neurospora crassa OR74A
    Definition Neurospora crassa OR74A dTDP-D-glucose 4,6-dehydratase, variant 2 (NCU00403), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_011395533.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGACTTTCG CAACACAAGA GCCAACCAGC CGGTACAACA GCGCAGTCAC GCCTATTTCA 
    CCCAGCTCCC AACTGGAGAG CCCTTTCGAG GTCAACGAAA TTTGGCGTGA TGCTCCTGTA 
    TTCACCGGCA CTACAAAGTT TGATCCTCTG CCTGATGTAA AGAACATCAT GGTAACAGGA 
    GGCGCTGGTT TCATAGCATG TTGGGTTGTC AGACACTTGG TATTGACGTA TCCAGAAGCC 
    TACAATGTCG TCTCATTCGA CAAGTTAGAC TACTGCGCAT CGCTCAACAA CACCAGGGTA 
    CTAGAACATG AGCCGAACTT CTCGTTTTAC AGAGGCGACA TTACGAATCC TTCCGAGGTC 
    ATGGACTGTC TGGAGCGATA CAATATCGAC ACCATCTTTC ACTTTGCCGC GCAATCACAT 
    GTCGATTTGA GTTTTGGAAA TCCGTTCGGT TTCACTCATA CCAATGTTTA CGGTACCCAT 
    GTTCTGCTCG AGAGCGCTCG AAAAGCGGGC ATCAAGCGCT TCATACACGT GTCTACCGAT 
    GAAGTATATG GCGAGGTAAA GGATGATGAA GATGATCTTC TGGAGACGAG CATTCTGGCA 
    CCCACTAACC CCTATGCGGC CAGCAAAGCT GCTGCCGAGA TGCTGGTGAA CTCTTACAAG 
    AAGAGCTTTA AATTACCCAT CATTATAGTG AGGAGCAACA ATGTCTATGG CCCTCACCAG 
    TACCCGGAGA AAATCATCCC AAAGTTCACT TGCCTACTCG CCCGAGGAGA GCCCGTCGTC 
    CTTCACGGAG ACGGCTCGCC CACTCGTCGT TATCTTTTTG CAGGCGACGC CGCCGACGCG 
    TTCGATACCA TTCTGCACAG AGGTCAGCTA GGGGAGATCT ATAACGTTGG TTCTTACGAT 
    GAAATCAGCA ACTTGTCGCT TTGCCACAAA CTCCTAACCG AGATGGAGAT CATCCCCTGT 
    CGGTCTCCGA CATCATCTCC TCCTTGCAAC TATACCACAT CCCCGCCCTC CTCGTCGCCT 
    CTGGGGGCAG AACAAGAAGA GATCCATCGT TGGGTCAAGT ACACTCACGA CCGACCTTTC 
    AACGATCACC GGTATGCCGT TGACGGTACG AAACTGCGAA AACTAGGTTG GGAGCCGAAG 
    ACAACTTTTG AAGAGGGGCT CAGGATCACT GTGGACTGGT ACAGACGGTT TGGGGAAAGG 
    TGGTGGGATG ATATTACCAA CGTATTGAGG CCCTTCCCTA TAACAGCGGG GGGCGAAGAC 
    GGGGTAATGG ATCAAGAGGT GGCCGAGATG TGTGACAAGC CCGTCACTTT GGAATCAAGA 
    GAGAATACGG GGGTCAGAAA GTCATCCGAT ATACTGAAGG AAGCATCGGG TTCGTTCAGG 
    TCACGCCACC AACAATATCA GCATCAACAT CATCACAAAC ACCGCACAAG AAAATATCTT 
    CACCACACAA GGGAAACGTC GCGAGACAAC ACGGATTATT ATTCTAGCAG GATACCAAGA 
    AGCTGCGCTC TAGCTTCTCC ACGCCCACGA GAGCTCGCCT CCAGCACCGG CCTTGTAAGC 
    CCAGGGATCA CATTAGATGA GGACGATGGT TTCGACGATG AGGATACGGC ATATAACCCA 
    CTAACGATCG TTTACGACGA CGAGGCACGA TCACGAAAGA AGCGCAAGCT TTCAATCGTG 
    GTCGATGATA TCACCAACGA TAGCCACCGC TTCAACAAGA AGGCCCGGGG AGATATTACT 
    CAAGAAGGGG GTCGCTGTGC TAAACTGTCG TGGACAGGCT TGACAAGAAC GTTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    NCU00403 ( XM_011395532.1 ) cDNA ORF clone, Neurospora crassa OR74A -> XP_011393834.1 Neurospora crassa OR74A dTDP-D-glucose 4,6-dehydratase, variant 1 (NCU00403), mRNA.
    Price & Availability↑
    CloneID ONf10345
    Clone ID Related Accession (Same CDS sequence) XM_011395533.1 , XM_011395532.1
    Accession Version XM_011395532.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1797bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-10
    Organism Neurospora crassa OR74A
    Product dTDP-D-glucose 4,6-dehydratase, variant 1
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026503). 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGACTTTCG CAACACAAGA GCCAACCAGC CGGTACAACA GCGCAGTCAC GCCTATTTCA 
    CCCAGCTCCC AACTGGAGAG CCCTTTCGAG GTCAACGAAA TTTGGCGTGA TGCTCCTGTA 
    TTCACCGGCA CTACAAAGTT TGATCCTCTG CCTGATGTAA AGAACATCAT GGTAACAGGA 
    GGCGCTGGTT TCATAGCATG TTGGGTTGTC AGACACTTGG TATTGACGTA TCCAGAAGCC 
    TACAATGTCG TCTCATTCGA CAAGTTAGAC TACTGCGCAT CGCTCAACAA CACCAGGGTA 
    CTAGAACATG AGCCGAACTT CTCGTTTTAC AGAGGCGACA TTACGAATCC TTCCGAGGTC 
    ATGGACTGTC TGGAGCGATA CAATATCGAC ACCATCTTTC ACTTTGCCGC GCAATCACAT 
    GTCGATTTGA GTTTTGGAAA TCCGTTCGGT TTCACTCATA CCAATGTTTA CGGTACCCAT 
    GTTCTGCTCG AGAGCGCTCG AAAAGCGGGC ATCAAGCGCT TCATACACGT GTCTACCGAT 
    GAAGTATATG GCGAGGTAAA GGATGATGAA GATGATCTTC TGGAGACGAG CATTCTGGCA 
    CCCACTAACC CCTATGCGGC CAGCAAAGCT GCTGCCGAGA TGCTGGTGAA CTCTTACAAG 
    AAGAGCTTTA AATTACCCAT CATTATAGTG AGGAGCAACA ATGTCTATGG CCCTCACCAG 
    TACCCGGAGA AAATCATCCC AAAGTTCACT TGCCTACTCG CCCGAGGAGA GCCCGTCGTC 
    CTTCACGGAG ACGGCTCGCC CACTCGTCGT TATCTTTTTG CAGGCGACGC CGCCGACGCG 
    TTCGATACCA TTCTGCACAG AGGTCAGCTA GGGGAGATCT ATAACGTTGG TTCTTACGAT 
    GAAATCAGCA ACTTGTCGCT TTGCCACAAA CTCCTAACCG AGATGGAGAT CATCCCCTGT 
    CGGTCTCCGA CATCATCTCC TCCTTGCAAC TATACCACAT CCCCGCCCTC CTCGTCGCCT 
    CTGGGGGCAG AACAAGAAGA GATCCATCGT TGGGTCAAGT ACACTCACGA CCGACCTTTC 
    AACGATCACC GGTATGCCGT TGACGGTACG AAACTGCGAA AACTAGGTTG GGAGCCGAAG 
    ACAACTTTTG AAGAGGGGCT CAGGATCACT GTGGACTGGT ACAGACGGTT TGGGGAAAGG 
    TGGTGGGATG ATATTACCAA CGTATTGAGG CCCTTCCCTA TAACAGCGGG GGGCGAAGAC 
    GGGGTAATGG ATCAAGAGGT GGCCGAGATG TGTGACAAGC CCGTCACTTT GGAATCAAGA 
    GAGAATACGG GGGTCAGAAA GTCATCCGAT ATACTGAAGG AAGCATCGGG TTCGTTCAGG 
    TCACGCCACC AACAATATCA GCATCAACAT CATCACAAAC ACCGCACAAG AAAATATCTT 
    CACCACACAA GGGAAACGTC GCGAGACAAC ACGGATTATT ATTCTAGCAG GATACCAAGA 
    AGCTGCGCTC TAGCTTCTCC ACGCCCACGA GAGCTCGCCT CCAGCACCGG CCTTGTAAGC 
    CCAGGGATCA CATTAGATGA GGACGATGGT TTCGACGATG AGGATACGGC ATATAACCCA 
    CTAACGATCG TTTACGACGA CGAGGCACGA TCACGAAAGA AGCGCAAGCT TTCAATCGTG 
    GTCGATGATA TCACCAACGA TAGCCACCGC TTCAACAAGA AGGCCCGGGG AGATATTACT 
    CAAGAAGGGG GTCGCTGTGC TAAACTGTCG TGGACAGGCT TGACAAGAAC GTTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011393834.1
    CDS725..2521
    Translation

    Target ORF information:

    RefSeq Version XM_011395532.1
    Organism Neurospora crassa OR74A
    Definition Neurospora crassa OR74A dTDP-D-glucose 4,6-dehydratase, variant 1 (NCU00403), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_011395532.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGACTTTCG CAACACAAGA GCCAACCAGC CGGTACAACA GCGCAGTCAC GCCTATTTCA 
    CCCAGCTCCC AACTGGAGAG CCCTTTCGAG GTCAACGAAA TTTGGCGTGA TGCTCCTGTA 
    TTCACCGGCA CTACAAAGTT TGATCCTCTG CCTGATGTAA AGAACATCAT GGTAACAGGA 
    GGCGCTGGTT TCATAGCATG TTGGGTTGTC AGACACTTGG TATTGACGTA TCCAGAAGCC 
    TACAATGTCG TCTCATTCGA CAAGTTAGAC TACTGCGCAT CGCTCAACAA CACCAGGGTA 
    CTAGAACATG AGCCGAACTT CTCGTTTTAC AGAGGCGACA TTACGAATCC TTCCGAGGTC 
    ATGGACTGTC TGGAGCGATA CAATATCGAC ACCATCTTTC ACTTTGCCGC GCAATCACAT 
    GTCGATTTGA GTTTTGGAAA TCCGTTCGGT TTCACTCATA CCAATGTTTA CGGTACCCAT 
    GTTCTGCTCG AGAGCGCTCG AAAAGCGGGC ATCAAGCGCT TCATACACGT GTCTACCGAT 
    GAAGTATATG GCGAGGTAAA GGATGATGAA GATGATCTTC TGGAGACGAG CATTCTGGCA 
    CCCACTAACC CCTATGCGGC CAGCAAAGCT GCTGCCGAGA TGCTGGTGAA CTCTTACAAG 
    AAGAGCTTTA AATTACCCAT CATTATAGTG AGGAGCAACA ATGTCTATGG CCCTCACCAG 
    TACCCGGAGA AAATCATCCC AAAGTTCACT TGCCTACTCG CCCGAGGAGA GCCCGTCGTC 
    CTTCACGGAG ACGGCTCGCC CACTCGTCGT TATCTTTTTG CAGGCGACGC CGCCGACGCG 
    TTCGATACCA TTCTGCACAG AGGTCAGCTA GGGGAGATCT ATAACGTTGG TTCTTACGAT 
    GAAATCAGCA ACTTGTCGCT TTGCCACAAA CTCCTAACCG AGATGGAGAT CATCCCCTGT 
    CGGTCTCCGA CATCATCTCC TCCTTGCAAC TATACCACAT CCCCGCCCTC CTCGTCGCCT 
    CTGGGGGCAG AACAAGAAGA GATCCATCGT TGGGTCAAGT ACACTCACGA CCGACCTTTC 
    AACGATCACC GGTATGCCGT TGACGGTACG AAACTGCGAA AACTAGGTTG GGAGCCGAAG 
    ACAACTTTTG AAGAGGGGCT CAGGATCACT GTGGACTGGT ACAGACGGTT TGGGGAAAGG 
    TGGTGGGATG ATATTACCAA CGTATTGAGG CCCTTCCCTA TAACAGCGGG GGGCGAAGAC 
    GGGGTAATGG ATCAAGAGGT GGCCGAGATG TGTGACAAGC CCGTCACTTT GGAATCAAGA 
    GAGAATACGG GGGTCAGAAA GTCATCCGAT ATACTGAAGG AAGCATCGGG TTCGTTCAGG 
    TCACGCCACC AACAATATCA GCATCAACAT CATCACAAAC ACCGCACAAG AAAATATCTT 
    CACCACACAA GGGAAACGTC GCGAGACAAC ACGGATTATT ATTCTAGCAG GATACCAAGA 
    AGCTGCGCTC TAGCTTCTCC ACGCCCACGA GAGCTCGCCT CCAGCACCGG CCTTGTAAGC 
    CCAGGGATCA CATTAGATGA GGACGATGGT TTCGACGATG AGGATACGGC ATATAACCCA 
    CTAACGATCG TTTACGACGA CGAGGCACGA TCACGAAAGA AGCGCAAGCT TTCAATCGTG 
    GTCGATGATA TCACCAACGA TAGCCACCGC TTCAACAAGA AGGCCCGGGG AGATATTACT 
    CAAGAAGGGG GTCGCTGTGC TAAACTGTCG TGGACAGGCT TGACAAGAAC GTTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The genome sequence of the filamentous fungus Neurospora crassa.
    Nature422(6934)859-68(2003 Apr)
    Galagan JE,Calvo SE,Borkovich KA,Selker EU,Read ND,Jaffe D,FitzHugh W,Ma LJ,Smirnov S,Purcell S,Rehman B,Elkins T,Engels R,Wang S,Nielsen CB,Butler J,Endrizzi M,Qui D,Ianakiev P,Bell-Pedersen D,Nelson MA,Werner-Washburne M,Selitrennikoff CP,Kinsey JA,Braun EL,Zelter A,Schulte U,Kothe GO,Jedd G,Mewes W,Staben C,Marcotte E,Greenberg D,Roy A,Foley K,Naylor J,Stange-Thomann N,Barrett R,Gnerre S,Kamal M,Kamvysselis M,Mauceli E,Bielke C,Rudd S,Frishman D,Krystofova S,Rasmussen C,Metzenberg RL,Perkins DD,Kroken S,Cogoni C,Macino G,Catcheside D,Li W,Pratt RJ,Osmani SA,DeSouza CP,Glass L,Orbach MJ,Berglund JA,Voelker R,Yarden O,Plamann M,Seiler S,Dunlap J,Radford A,Aramayo R,Natvig DO,Alex LA,Mannhaupt G,Ebbole DJ,Freitag M,Paulsen I,Sachs MS,Lander ES,Nusbaum C,Birren B


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