NCU00396 cDNA ORF clone, Neurospora crassa OR74A

Gene
Clones
gRNAs

Summary

Gene Symbol	NCU00396
Entrez Gene ID	3873404
Full Name	pre-mRNA-splicing factor rse-1
Gene Type	protein-coding
Organism	Neurospora crassa OR74A

mRNA and Protein(s)

mRNA	Protein	Name
XM_011395525.1	XP_011393827.1	pre-mRNA-splicing factor rse-1
XM_011395526.1	XP_011393828.1	pre-mRNA-splicing factor rse-1, variant

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	SF3B3	XP_536791.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005549	XP_315551.1
Homo sapiens (human)	SF3B3	NP_036558.3
Bos taurus (cattle)	SF3B3	NP_001071319.1
Rattus norvegicus (Norway rat)	Sf3b3	NP_001099657.2
Gallus gallus (chicken)	SF3B3	XP_004944176.1
Danio rerio (zebrafish)	LOC100334114	XP_002667683.2
Magnaporthe oryzae (rice blast fungus)	MGG_06457	XP_003717109.1
Neurospora crassa	NCU00396	XP_957282.2
Xenopus tropicalis (tropical clawed frog)	sf3b3	XP_002931705.1
	SF3B3	XP_002802591.1
Mus musculus (house mouse)	Sf3b3	NP_598714.1
Caenorhabditis elegans (roundworm)	teg-4	NP_491953.1
Arabidopsis thaliana (thale cress)	AT3G55220	NP_567016.1
Pan troglodytes (chimpanzee)	SF3B3	XP_511081.3
Danio rerio (zebrafish)	sf3b3	NP_998668.1
Drosophila melanogaster (fruit fly)	CG13900	NP_728546.1
Schizosaccharomyces pombe (fission yeast)	prp12	NP_594414.1
Arabidopsis thaliana (thale cress)	AT3G55200	NP_567015.1
Oryza sativa (rice)	Os02g0137400	NP_001045829.2

Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Galagan JE, Calvo SE, Borkovich KA, Selker EU, Read ND, Jaffe D, FitzHugh W, Ma LJ, Smirnov S, Purcell S, Rehman B, Elkins T, Engels R, Wang S, Nielsen CB, Butler J, Endrizzi M, Qui D, Ianakiev P, Bell-Pedersen D, Nelson MA, Werner-Washburne M, Selitrennikoff CP, Kinsey JA, Braun EL, Zelter A, Schulte U, Kothe GO, Jedd G, Mewes W, Staben C, Marcotte E, Greenberg D, Roy A, Foley K, Naylor J, Stange-Thomann N, Barrett R, Gnerre S, Kamal M, Kamvysselis M, Mauceli E, Bielke C, Rudd S, Frishman D, Krystofova S, Rasmussen C, Metzenberg RL, Perkins DD, Kroken S, Cogoni C, Macino G, Catcheside D, Li W, Pratt RJ, Osmani SA, DeSouza CP, Glass L, Orbach MJ, Berglund JA, Voelker R, Yarden O, Plamann M, Seiler S, Dunlap J, Radford A, Aramayo R, Natvig DO, Alex LA, Mannhaupt G, Ebbole DJ, Freitag M, Paulsen I, Sachs MS, Lander ES, Nusbaum C, Birren B
Nature422(6934)859-68(2003 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following NCU00396 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NCU00396 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ONf10340	XM_011395526.1 Latest version!	Neurospora crassa OR74A pre-mRNA-splicing factor rse-1, variant (NCU00396), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
ONf10339	XM_011395525.1 Latest version!	Neurospora crassa OR74A pre-mRNA-splicing factor rse-1 (NCU00396), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NCU00396 ( XM_011395526.1 ) cDNA ORF clone, Neurospora crassa OR74A -> XP_011393828.1 Neurospora crassa OR74A pre-mRNA-splicing factor rse-1, variant (NCU00396), mRNA.

Price & Availability↑

CloneID	ONf10340
Clone ID Related Accession (Same CDS sequence)	XM_011395526.1
Accession Version	XM_011395526.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2742bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-10
Organism	Neurospora crassa OR74A
Product	pre-mRNA-splicing factor rse-1, variant
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026503).

ATGCACAAGC TCAAGGGAAG CGCGGGCGCC TTTTTCTTCC TTCTTCAGAC GGACGATGGA 
GATCTCTTCA AAGTCACCAT TGACATGATC GAAGATAGTG ACGGCAACCC CACCGGAGAG 
GTCAAGCGCC TCAAGATCAA GTACTTCGAC ACGATACCAG TAGCCACTAG TCTTTGCATC 
CTGAAGAGCG GCTTCCTGTT CGCCGCTAGC GAGTTTGGAA ACCATCACTT TTACCAATTT 
GAAAAGCTCG GAGACGATGA CGAGGAGCTC GAGTTCAGCA GCGACGACTT CCCGACAGAC 
CCTACCGCCT CATATAACCC GGTGTACTTC CACCCCAGGC CACTTGAAAA TCTGGTCCTG 
GTTGAGAGCA TTGATTCGAT GAACCCTCAG GTCGACTGCA AGGTTGCGAA TCTGACGGGA 
GAGGACGCGC CCCAGATCTA CTCTGTCTGT GGAAATGGGG CGCGGAGTAC TTTCCGCATG 
TTGAAGCACG GACTCGAAGT GTCTGAAATT GTCGCTTCGG AGCTTCCGGG TACACCTTCT 
GCTGTATGGA CGACTAAGTT GACCAAGTAC GATCAGTACG ATGCTTATAT TGTGCTGTCA 
TTCACAAATG GAACATTGGT TCTCAGCATT GGTGAGACGG TAGAAGAGGT CAGCGATAGT 
GGTTTCTTGA CTACGGCGCC CACCCTAGCT GTGCAGCAAA TGGGTGAGGA TGGCTTGATA 
CAAGTTCACC CCAAGGGCAT TCGCCACATC GTCCAAGGTC GTGTCAATGA ATGGCCTGCA 
CCTCAGCATC GCTCGATTGT TGCTGCCACG GCAAATGAGA ATCAGGTTGT CATCGCCTTG 
AGCTCTGGCG AAATCGTCTA CTTCGAAATG GACTCGGATG GTTCGCTCGC CGAGTATGAC 
GAAAAGAAGG AGATGTCGGG TACCGTCACA TCGCTGAGCG TCGGTCAAGT ACCGGAGGGC 
CTGAAGCGAA GCTCTTTCCT AGCTGTAGGA TGCGATGATT GCACAGTTCG CATTCTTAGC 
TTGGACCCTG ATTCAACACT CGAGATGAAG TCCATCCAAG CTTTAACAGC CGCGCCCTCG 
GCTTTATCAA TCATGTCGAT GGAGGACTCG TTTGGTGGAT CCACGCTCTA TCTCCACATC 
GGATTGCATT CGGGCGTTTA CCTCCGGACT GTGCTGGATG AAGTGACGGG CGAACTGACA 
GACACTCGTC AAAAATTCTT GGGTCCCAAG CCTACCAGAC TTTTCCAAGT ATCAGTTCAG 
GATCAGCCAT GTGTTCTTGC GCTGAGCTCG AGGCCATGGT TAGGCTACAC CGACCCACTT 
ACTAAGGGCT TCATGATGAC GCCCCTAAGT TACACCGAGC TTGAGTATGG TTGGAACTTC 
AGTAGCGAAC AGTGCTTGGA AGGCATGGTT GGTATTCATG CCAACTACCT AAGAATCTTT 
TCAATCGAAA AACTGGGCGA CAACATGATT CAGAAATCCA TTCCTTTGAC TTACACACCT 
AAGCACTTGG TCAAGCATCC CGAACAGCCA TACTTTTACA CTATCGAATC CGACAATAAC 
ACCCTCCCCC CGGAGCTGCG GGCAAAGCTT CTGGAGCAAC AAAGCAATGG CGACGCAACG 
GTACTGCCGC CAGAGGACTT TGGATATCCC AGAGCCAAAG GTAGATGGGC ATCGTGCATC 
AGCATTATCG ACCCTATCAG TGAGGAACCG AGAGTCCTCC AAAGGATTGA TCTTGACAAC 
AATGAGGCGG CAGTCAGCGC GGCCATTGTA CCTTTTGCCA GTCAAGAGGG CGAAAGTTTC 
CTGGTTGTCG GCACGGGCAA AGATATGGTC CTCGACCCAC GTCAATTTAC CGAGGGCTAC 
ATCCATGTTT ACAGATTCCA TGAAGACGGC CGGGACCTGG AGTTCATTCA TAAGACCCGA 
GTAGAAGAGC CGCCTTTGGC ACTCATTCCG TTCCAAGGAC GTCTGCTTGC TGGCGTCGGC 
AAGACACTCC GTATCTACGA CCTCGGTTTG AAGCAACTGC TCCGAAAAGC TCAAGCAGAT 
GTGACGCCCA CTCTCATCGT CTCCCTACAA AGCCAGGGTA ACCGCATCAT TGTCGGCGAT 
CTCCAGCAAG GCATCACGTA CGTTGTCTAC AAGGCCGAGG GCAATAGACT CATTCCCTTT 
GCTGACGACA CCTTGAACCG CTGGACAACC TGCACCACCA TGGTCGACTA CGAATCCGTT 
GCCGGCGGCG ACAAGTTCGG CAACATATAC ATCGTCCGTT GCCCAGAGCG CGTCAGCCAG 
GAAACTGACG AGCCTGGATC AGAGATCCAC CTGATGCACG CCCGCAACTA CCTTCATGGC 
ACACCCAACC GCTTGAGCCT CCAGGTCCAC TTCTACACTC AGGACTTGCC GACCAGCATA 
TGCAAGACCA GCCTAGTTGT CGGTGGTCAG GACGTGCTGC TCTGGAGCGG CTTGCAGGGC 
ACTGTCGGTG TTTTCATTCC CTTTGTCAGC CGTGAGGATG TCGACTTCTT CCAGAATCTG 
GAGAATCATA TGCGCGCTGA AGACCCGCCG CTTGCCGGCC GTGATCATTT GATCTACCGG 
GGTTACTATA CCCCCGTCAA GGGTGTGATC GATGGTGACT TGTGCGAGAG GTTTAGCTTG 
CTGCCGAATG ATAAGAAGCA GATGATTGCG GGAGAACTTG ATCGCTCGGT GAGGGAAATC 
GAGCGCAAGA TTTCGGATAT CCGCACCCGG TCTGCCTTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011393828.1
CDS	1298..4039
Translation	MHKLKGSAGAFFFLLQTDDGDLFKVTIDMIEDSDGNPTGEVKRLKIKYFDTIPVATSLCILKSGFLFAASEFGNHHFYQFEKLGDDDEELEFSSDDFPTDPTASYNPVYFHPRPLENLVLVESIDSMNPQVDCKVANLTGEDAPQIYSVCGNGARSTFRMLKHGLEVSEIVASELPGTPSAVWTTKLTKYDQYDAYIVLSFTNGTLVLSIGETVEEVSDSGFLTTAPTLAVQQMGEDGLIQVHPKGIRHIVQGRVNEWPAPQHRSIVAATANENQVVIALSSGEIVYFEMDSDGSLAEYDEKKEMSGTVTSLSVGQVPEGLKRSSFLAVGCDDCTVRILSLDPDSTLEMKSIQALTAAPSALSIMSMEDSFGGSTLYLHIGLHSGVYLRTVLDEVTGELTDTRQKFLGPKPTRLFQVSVQDQPCVLALSSRPWLGYTDPLTKGFMMTPLSYTELEYGWNFSSEQCLEGMVGIHANYLRIFSIEKLGDNMIQKSIPLTYTPKHLVKHPEQPYFYTIESDNNTLPPELRAKLLEQQSNGDATVLPPEDFGYPRAKGRWASCISIIDPISEEPRVLQRIDLDNNEAAVSAAIVPFASQEGESFLVVGTGKDMVLDPRQFTEGYIHVYRFHEDGRDLEFIHKTRVEEPPLALIPFQGRLLAGVGKTLRIYDLGLKQLLRKAQADVTPTLIVSLQSQGNRIIVGDLQQGITYVVYKAEGNRLIPFADDTLNRWTTCTTMVDYESVAGGDKFGNIYIVRCPERVSQETDEPGSEIHLMHARNYLHGTPNRLSLQVHFYTQDLPTSICKTSLVVGGQDVLLWSGLQGTVGVFIPFVSREDVDFFQNLENHMRAEDPPLAGRDHLIYRGYYTPVKGVIDGDLCERFSLLPNDKKQMIAGELDRSVREIERKISDIRTRSAF

Target ORF information:

RefSeq Version	XM_011395526.1
Organism	Neurospora crassa OR74A
Definition	Neurospora crassa OR74A pre-mRNA-splicing factor rse-1, variant (NCU00396), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011395526.1

ORF Insert Sequence:

ATGCACAAGC TCAAGGGAAG CGCGGGCGCC TTTTTCTTCC TTCTTCAGAC GGACGATGGA 
GATCTCTTCA AAGTCACCAT TGACATGATC GAAGATAGTG ACGGCAACCC CACCGGAGAG 
GTCAAGCGCC TCAAGATCAA GTACTTCGAC ACGATACCAG TAGCCACTAG TCTTTGCATC 
CTGAAGAGCG GCTTCCTGTT CGCCGCTAGC GAGTTTGGAA ACCATCACTT TTACCAATTT 
GAAAAGCTCG GAGACGATGA CGAGGAGCTC GAGTTCAGCA GCGACGACTT CCCGACAGAC 
CCTACCGCCT CATATAACCC GGTGTACTTC CACCCCAGGC CACTTGAAAA TCTGGTCCTG 
GTTGAGAGCA TTGATTCGAT GAACCCTCAG GTCGACTGCA AGGTTGCGAA TCTGACGGGA 
GAGGACGCGC CCCAGATCTA CTCTGTCTGT GGAAATGGGG CGCGGAGTAC TTTCCGCATG 
TTGAAGCACG GACTCGAAGT GTCTGAAATT GTCGCTTCGG AGCTTCCGGG TACACCTTCT 
GCTGTATGGA CGACTAAGTT GACCAAGTAC GATCAGTACG ATGCTTATAT TGTGCTGTCA 
TTCACAAATG GAACATTGGT TCTCAGCATT GGTGAGACGG TAGAAGAGGT CAGCGATAGT 
GGTTTCTTGA CTACGGCGCC CACCCTAGCT GTGCAGCAAA TGGGTGAGGA TGGCTTGATA 
CAAGTTCACC CCAAGGGCAT TCGCCACATC GTCCAAGGTC GTGTCAATGA ATGGCCTGCA 
CCTCAGCATC GCTCGATTGT TGCTGCCACG GCAAATGAGA ATCAGGTTGT CATCGCCTTG 
AGCTCTGGCG AAATCGTCTA CTTCGAAATG GACTCGGATG GTTCGCTCGC CGAGTATGAC 
GAAAAGAAGG AGATGTCGGG TACCGTCACA TCGCTGAGCG TCGGTCAAGT ACCGGAGGGC 
CTGAAGCGAA GCTCTTTCCT AGCTGTAGGA TGCGATGATT GCACAGTTCG CATTCTTAGC 
TTGGACCCTG ATTCAACACT CGAGATGAAG TCCATCCAAG CTTTAACAGC CGCGCCCTCG 
GCTTTATCAA TCATGTCGAT GGAGGACTCG TTTGGTGGAT CCACGCTCTA TCTCCACATC 
GGATTGCATT CGGGCGTTTA CCTCCGGACT GTGCTGGATG AAGTGACGGG CGAACTGACA 
GACACTCGTC AAAAATTCTT GGGTCCCAAG CCTACCAGAC TTTTCCAAGT ATCAGTTCAG 
GATCAGCCAT GTGTTCTTGC GCTGAGCTCG AGGCCATGGT TAGGCTACAC CGACCCACTT 
ACTAAGGGCT TCATGATGAC GCCCCTAAGT TACACCGAGC TTGAGTATGG TTGGAACTTC 
AGTAGCGAAC AGTGCTTGGA AGGCATGGTT GGTATTCATG CCAACTACCT AAGAATCTTT 
TCAATCGAAA AACTGGGCGA CAACATGATT CAGAAATCCA TTCCTTTGAC TTACACACCT 
AAGCACTTGG TCAAGCATCC CGAACAGCCA TACTTTTACA CTATCGAATC CGACAATAAC 
ACCCTCCCCC CGGAGCTGCG GGCAAAGCTT CTGGAGCAAC AAAGCAATGG CGACGCAACG 
GTACTGCCGC CAGAGGACTT TGGATATCCC AGAGCCAAAG GTAGATGGGC ATCGTGCATC 
AGCATTATCG ACCCTATCAG TGAGGAACCG AGAGTCCTCC AAAGGATTGA TCTTGACAAC 
AATGAGGCGG CAGTCAGCGC GGCCATTGTA CCTTTTGCCA GTCAAGAGGG CGAAAGTTTC 
CTGGTTGTCG GCACGGGCAA AGATATGGTC CTCGACCCAC GTCAATTTAC CGAGGGCTAC 
ATCCATGTTT ACAGATTCCA TGAAGACGGC CGGGACCTGG AGTTCATTCA TAAGACCCGA 
GTAGAAGAGC CGCCTTTGGC ACTCATTCCG TTCCAAGGAC GTCTGCTTGC TGGCGTCGGC 
AAGACACTCC GTATCTACGA CCTCGGTTTG AAGCAACTGC TCCGAAAAGC TCAAGCAGAT 
GTGACGCCCA CTCTCATCGT CTCCCTACAA AGCCAGGGTA ACCGCATCAT TGTCGGCGAT 
CTCCAGCAAG GCATCACGTA CGTTGTCTAC AAGGCCGAGG GCAATAGACT CATTCCCTTT 
GCTGACGACA CCTTGAACCG CTGGACAACC TGCACCACCA TGGTCGACTA CGAATCCGTT 
GCCGGCGGCG ACAAGTTCGG CAACATATAC ATCGTCCGTT GCCCAGAGCG CGTCAGCCAG 
GAAACTGACG AGCCTGGATC AGAGATCCAC CTGATGCACG CCCGCAACTA CCTTCATGGC 
ACACCCAACC GCTTGAGCCT CCAGGTCCAC TTCTACACTC AGGACTTGCC GACCAGCATA 
TGCAAGACCA GCCTAGTTGT CGGTGGTCAG GACGTGCTGC TCTGGAGCGG CTTGCAGGGC 
ACTGTCGGTG TTTTCATTCC CTTTGTCAGC CGTGAGGATG TCGACTTCTT CCAGAATCTG 
GAGAATCATA TGCGCGCTGA AGACCCGCCG CTTGCCGGCC GTGATCATTT GATCTACCGG 
GGTTACTATA CCCCCGTCAA GGGTGTGATC GATGGTGACT TGTGCGAGAG GTTTAGCTTG 
CTGCCGAATG ATAAGAAGCA GATGATTGCG GGAGAACTTG ATCGCTCGGT GAGGGAAATC 
GAGCGCAAGA TTTCGGATAT CCGCACCCGG TCTGCCTTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NCU00396 ( XM_011395525.1 ) cDNA ORF clone, Neurospora crassa OR74A -> XP_011393827.1 Neurospora crassa OR74A pre-mRNA-splicing factor rse-1 (NCU00396), mRNA.

Price & Availability↑

CloneID	ONf10339
Clone ID Related Accession (Same CDS sequence)	XM_011395525.1
Accession Version	XM_011395525.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3630bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-10
Organism	Neurospora crassa OR74A
Product	pre-mRNA-splicing factor rse-1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026503).

ATGGCGACTA CGTCGAACAT GTTCCTGTAT TCCTTGACCA TCCAGCCACC CACCGCTGTC 
ACGCAGGCTC TGCTTGGTCA GTTCTCGGGA ACCAAGGAAC AGCAGATTCT TACGGCGTCT 
GGATCGCGTC TAACCCTCCT TCAGCCTGAT CCCCGTCAGG GCAAGGTCAA CACCCTGTTG 
TCTCATGACA TCTTCGGCAT CGTACGCGCC ATCGCGTCGT TCCGTCTGGC CGGCAGCCAC 
AAGGACTACA TTATCCTCGC CACTGATTCG GGCCGCATTA CGATTATCGA GTATCTCCCC 
AAGACGAACA AGTTTCAGCG CATCCACCTC GAGACTTTCG GCAAATCGGG CGTCCGCCGA 
GTCATCCCCG GCCAGTACTT AGCCGCAGAT CCAAAGGGAA GAGCATGCCT CATTTCGGCA 
CTCGAAAAGA ACAAGCTGGT CTATGTTCTC AACCGAAACT CACAGGCCGA GCTTACCATC 
TCGTCACCAC TGGAAGCCCA CAAGCCAGGC GTGTTGGTGC TGTCTCTTGT TGCCCTTGAC 
GTTGGTTACG CAAACCCCGT TTTCGCTGCG TTGGAACTGG ACTACACGGA CGCGGACCAA 
GATCCTACGG GCCAAGCACG AGAAGAAGTC GAGACACAGC TGGTATATTA CGAGCTTGAT 
CTGGGCCTGA ACCACGTTGT ACGCAAGTGG TCAGATACTG TCGATCGCAC TTCTTCACTT 
CTCTTCCAAG TACCCGGCGG CAATGACGGT CCCAGCGGTG TCTTGGTGTG CGGCGAGGAA 
AATGTCACCT ATCGGCACTC GAATCAAGAG GCTTTCCGCG TCCCCATCCC TAGACGCAGC 
GGTGCCACCG AAGATCCTCA GAGGAAGCGG GTTATCGTCT CTGGCGTTAT GCACAAGCTC 
AAGGGAAGCG CGGGCGCCTT TTTCTTCCTT CTTCAGACGG ACGATGGAGA TCTCTTCAAA 
GTCACCATTG ACATGATCGA AGATAGTGAC GGCAACCCCA CCGGAGAGGT CAAGCGCCTC 
AAGATCAAGT ACTTCGACAC GATACCAGTA GCCACTAGTC TTTGCATCCT GAAGAGCGGC 
TTCCTGTTCG CCGCTAGCGA GTTTGGAAAC CATCACTTTT ACCAATTTGA AAAGCTCGGA 
GACGATGACG AGGAGCTCGA GTTCAGCAGC GACGACTTCC CGACAGACCC TACCGCCTCA 
TATAACCCGG TGTACTTCCA CCCCAGGCCA CTTGAAAATC TGGTCCTGGT TGAGAGCATT 
GATTCGATGA ACCCTCAGGT CGACTGCAAG GTTGCGAATC TGACGGGAGA GGACGCGCCC 
CAGATCTACT CTGTCTGTGG AAATGGGGCG CGGAGTACTT TCCGCATGTT GAAGCACGGA 
CTCGAAGTGT CTGAAATTGT CGCTTCGGAG CTTCCGGGTA CACCTTCTGC TGTATGGACG 
ACTAAGTTGA CCAAGTACGA TCAGTACGAT GCTTATATTG TGCTGTCATT CACAAATGGA 
ACATTGGTTC TCAGCATTGG TGAGACGGTA GAAGAGGTCA GCGATAGTGG TTTCTTGACT 
ACGGCGCCCA CCCTAGCTGT GCAGCAAATG GGTGAGGATG GCTTGATACA AGTTCACCCC 
AAGGGCATTC GCCACATCGT CCAAGGTCGT GTCAATGAAT GGCCTGCACC TCAGCATCGC 
TCGATTGTTG CTGCCACGGC AAATGAGAAT CAGGTTGTCA TCGCCTTGAG CTCTGGCGAA 
ATCGTCTACT TCGAAATGGA CTCGGATGGT TCGCTCGCCG AGTATGACGA AAAGAAGGAG 
ATGTCGGGTA CCGTCACATC GCTGAGCGTC GGTCAAGTAC CGGAGGGCCT GAAGCGAAGC 
TCTTTCCTAG CTGTAGGATG CGATGATTGC ACAGTTCGCA TTCTTAGCTT GGACCCTGAT 
TCAACACTCG AGATGAAGTC CATCCAAGCT TTAACAGCCG CGCCCTCGGC TTTATCAATC 
ATGTCGATGG AGGACTCGTT TGGTGGATCC ACGCTCTATC TCCACATCGG ATTGCATTCG 
GGCGTTTACC TCCGGACTGT GCTGGATGAA GTGACGGGCG AACTGACAGA CACTCGTCAA 
AAATTCTTGG GTCCCAAGCC TACCAGACTT TTCCAAGTAT CAGTTCAGGA TCAGCCATGT 
GTTCTTGCGC TGAGCTCGAG GCCATGGTTA GGCTACACCG ACCCACTTAC TAAGGGCTTC 
ATGATGACGC CCCTAAGTTA CACCGAGCTT GAGTATGGTT GGAACTTCAG TAGCGAACAG 
TGCTTGGAAG GCATGGTTGG TATTCATGCC AACTACCTAA GAATCTTTTC AATCGAAAAA 
CTGGGCGACA ACATGATTCA GAAATCCATT CCTTTGACTT ACACACCTAA GCACTTGGTC 
AAGCATCCCG AACAGCCATA CTTTTACACT ATCGAATCCG ACAATAACAC CCTCCCCCCG 
GAGCTGCGGG CAAAGCTTCT GGAGCAACAA AGCAATGGCG ACGCAACGGT ACTGCCGCCA 
GAGGACTTTG GATATCCCAG AGCCAAAGGT AGATGGGCAT CGTGCATCAG CATTATCGAC 
CCTATCAGTG AGGAACCGAG AGTCCTCCAA AGGATTGATC TTGACAACAA TGAGGCGGCA 
GTCAGCGCGG CCATTGTACC TTTTGCCAGT CAAGAGGGCG AAAGTTTCCT GGTTGTCGGC 
ACGGGCAAAG ATATGGTCCT CGACCCACGT CAATTTACCG AGGGCTACAT CCATGTTTAC 
AGATTCCATG AAGACGGCCG GGACCTGGAG TTCATTCATA AGACCCGAGT AGAAGAGCCG 
CCTTTGGCAC TCATTCCGTT CCAAGGACGT CTGCTTGCTG GCGTCGGCAA GACACTCCGT 
ATCTACGACC TCGGTTTGAA GCAACTGCTC CGAAAAGCTC AAGCAGATGT GACGCCCACT 
CTCATCGTCT CCCTACAAAG CCAGGGTAAC CGCATCATTG TCGGCGATCT CCAGCAAGGC 
ATCACGTACG TTGTCTACAA GGCCGAGGGC AATAGACTCA TTCCCTTTGC TGACGACACC 
TTGAACCGCT GGACAACCTG CACCACCATG GTCGACTACG AATCCGTTGC CGGCGGCGAC 
AAGTTCGGCA ACATATACAT CGTCCGTTGC CCAGAGCGCG TCAGCCAGGA AACTGACGAG 
CCTGGATCAG AGATCCACCT GATGCACGCC CGCAACTACC TTCATGGCAC ACCCAACCGC 
TTGAGCCTCC AGGTCCACTT CTACACTCAG GACTTGCCGA CCAGCATATG CAAGACCAGC 
CTAGTTGTCG GTGGTCAGGA CGTGCTGCTC TGGAGCGGCT TGCAGGGCAC TGTCGGTGTT 
TTCATTCCCT TTGTCAGCCG TGAGGATGTC GACTTCTTCC AGAATCTGGA GAATCATATG 
CGCGCTGAAG ACCCGCCGCT TGCCGGCCGT GATCATTTGA TCTACCGGGG TTACTATACC 
CCCGTCAAGG GTGTGATCGA TGGTGACTTG TGCGAGAGGT TTAGCTTGCT GCCGAATGAT 
AAGAAGCAGA TGATTGCGGG AGAACTTGAT CGCTCGGTGA GGGAAATCGA GCGCAAGATT 
TCGGATATCC GCACCCGGTC TGCCTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011393827.1
CDS	283..3912
Translation	MATTSNMFLYSLTIQPPTAVTQALLGQFSGTKEQQILTASGSRLTLLQPDPRQGKVNTLLSHDIFGIVRAIASFRLAGSHKDYIILATDSGRITIIEYLPKTNKFQRIHLETFGKSGVRRVIPGQYLAADPKGRACLISALEKNKLVYVLNRNSQAELTISSPLEAHKPGVLVLSLVALDVGYANPVFAALELDYTDADQDPTGQAREEVETQLVYYELDLGLNHVVRKWSDTVDRTSSLLFQVPGGNDGPSGVLVCGEENVTYRHSNQEAFRVPIPRRSGATEDPQRKRVIVSGVMHKLKGSAGAFFFLLQTDDGDLFKVTIDMIEDSDGNPTGEVKRLKIKYFDTIPVATSLCILKSGFLFAASEFGNHHFYQFEKLGDDDEELEFSSDDFPTDPTASYNPVYFHPRPLENLVLVESIDSMNPQVDCKVANLTGEDAPQIYSVCGNGARSTFRMLKHGLEVSEIVASELPGTPSAVWTTKLTKYDQYDAYIVLSFTNGTLVLSIGETVEEVSDSGFLTTAPTLAVQQMGEDGLIQVHPKGIRHIVQGRVNEWPAPQHRSIVAATANENQVVIALSSGEIVYFEMDSDGSLAEYDEKKEMSGTVTSLSVGQVPEGLKRSSFLAVGCDDCTVRILSLDPDSTLEMKSIQALTAAPSALSIMSMEDSFGGSTLYLHIGLHSGVYLRTVLDEVTGELTDTRQKFLGPKPTRLFQVSVQDQPCVLALSSRPWLGYTDPLTKGFMMTPLSYTELEYGWNFSSEQCLEGMVGIHANYLRIFSIEKLGDNMIQKSIPLTYTPKHLVKHPEQPYFYTIESDNNTLPPELRAKLLEQQSNGDATVLPPEDFGYPRAKGRWASCISIIDPISEEPRVLQRIDLDNNEAAVSAAIVPFASQEGESFLVVGTGKDMVLDPRQFTEGYIHVYRFHEDGRDLEFIHKTRVEEPPLALIPFQGRLLAGVGKTLRIYDLGLKQLLRKAQADVTPTLIVSLQSQGNRIIVGDLQQGITYVVYKAEGNRLIPFADDTLNRWTTCTTMVDYESVAGGDKFGNIYIVRCPERVSQETDEPGSEIHLMHARNYLHGTPNRLSLQVHFYTQDLPTSICKTSLVVGGQDVLLWSGLQGTVGVFIPFVSREDVDFFQNLENHMRAEDPPLAGRDHLIYRGYYTPVKGVIDGDLCERFSLLPNDKKQMIAGELDRSVREIERKISDIRTRSAF

Target ORF information:

RefSeq Version	XM_011395525.1
Organism	Neurospora crassa OR74A
Definition	Neurospora crassa OR74A pre-mRNA-splicing factor rse-1 (NCU00396), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011395525.1

ORF Insert Sequence:

ATGGCGACTA CGTCGAACAT GTTCCTGTAT TCCTTGACCA TCCAGCCACC CACCGCTGTC 
ACGCAGGCTC TGCTTGGTCA GTTCTCGGGA ACCAAGGAAC AGCAGATTCT TACGGCGTCT 
GGATCGCGTC TAACCCTCCT TCAGCCTGAT CCCCGTCAGG GCAAGGTCAA CACCCTGTTG 
TCTCATGACA TCTTCGGCAT CGTACGCGCC ATCGCGTCGT TCCGTCTGGC CGGCAGCCAC 
AAGGACTACA TTATCCTCGC CACTGATTCG GGCCGCATTA CGATTATCGA GTATCTCCCC 
AAGACGAACA AGTTTCAGCG CATCCACCTC GAGACTTTCG GCAAATCGGG CGTCCGCCGA 
GTCATCCCCG GCCAGTACTT AGCCGCAGAT CCAAAGGGAA GAGCATGCCT CATTTCGGCA 
CTCGAAAAGA ACAAGCTGGT CTATGTTCTC AACCGAAACT CACAGGCCGA GCTTACCATC 
TCGTCACCAC TGGAAGCCCA CAAGCCAGGC GTGTTGGTGC TGTCTCTTGT TGCCCTTGAC 
GTTGGTTACG CAAACCCCGT TTTCGCTGCG TTGGAACTGG ACTACACGGA CGCGGACCAA 
GATCCTACGG GCCAAGCACG AGAAGAAGTC GAGACACAGC TGGTATATTA CGAGCTTGAT 
CTGGGCCTGA ACCACGTTGT ACGCAAGTGG TCAGATACTG TCGATCGCAC TTCTTCACTT 
CTCTTCCAAG TACCCGGCGG CAATGACGGT CCCAGCGGTG TCTTGGTGTG CGGCGAGGAA 
AATGTCACCT ATCGGCACTC GAATCAAGAG GCTTTCCGCG TCCCCATCCC TAGACGCAGC 
GGTGCCACCG AAGATCCTCA GAGGAAGCGG GTTATCGTCT CTGGCGTTAT GCACAAGCTC 
AAGGGAAGCG CGGGCGCCTT TTTCTTCCTT CTTCAGACGG ACGATGGAGA TCTCTTCAAA 
GTCACCATTG ACATGATCGA AGATAGTGAC GGCAACCCCA CCGGAGAGGT CAAGCGCCTC 
AAGATCAAGT ACTTCGACAC GATACCAGTA GCCACTAGTC TTTGCATCCT GAAGAGCGGC 
TTCCTGTTCG CCGCTAGCGA GTTTGGAAAC CATCACTTTT ACCAATTTGA AAAGCTCGGA 
GACGATGACG AGGAGCTCGA GTTCAGCAGC GACGACTTCC CGACAGACCC TACCGCCTCA 
TATAACCCGG TGTACTTCCA CCCCAGGCCA CTTGAAAATC TGGTCCTGGT TGAGAGCATT 
GATTCGATGA ACCCTCAGGT CGACTGCAAG GTTGCGAATC TGACGGGAGA GGACGCGCCC 
CAGATCTACT CTGTCTGTGG AAATGGGGCG CGGAGTACTT TCCGCATGTT GAAGCACGGA 
CTCGAAGTGT CTGAAATTGT CGCTTCGGAG CTTCCGGGTA CACCTTCTGC TGTATGGACG 
ACTAAGTTGA CCAAGTACGA TCAGTACGAT GCTTATATTG TGCTGTCATT CACAAATGGA 
ACATTGGTTC TCAGCATTGG TGAGACGGTA GAAGAGGTCA GCGATAGTGG TTTCTTGACT 
ACGGCGCCCA CCCTAGCTGT GCAGCAAATG GGTGAGGATG GCTTGATACA AGTTCACCCC 
AAGGGCATTC GCCACATCGT CCAAGGTCGT GTCAATGAAT GGCCTGCACC TCAGCATCGC 
TCGATTGTTG CTGCCACGGC AAATGAGAAT CAGGTTGTCA TCGCCTTGAG CTCTGGCGAA 
ATCGTCTACT TCGAAATGGA CTCGGATGGT TCGCTCGCCG AGTATGACGA AAAGAAGGAG 
ATGTCGGGTA CCGTCACATC GCTGAGCGTC GGTCAAGTAC CGGAGGGCCT GAAGCGAAGC 
TCTTTCCTAG CTGTAGGATG CGATGATTGC ACAGTTCGCA TTCTTAGCTT GGACCCTGAT 
TCAACACTCG AGATGAAGTC CATCCAAGCT TTAACAGCCG CGCCCTCGGC TTTATCAATC 
ATGTCGATGG AGGACTCGTT TGGTGGATCC ACGCTCTATC TCCACATCGG ATTGCATTCG 
GGCGTTTACC TCCGGACTGT GCTGGATGAA GTGACGGGCG AACTGACAGA CACTCGTCAA 
AAATTCTTGG GTCCCAAGCC TACCAGACTT TTCCAAGTAT CAGTTCAGGA TCAGCCATGT 
GTTCTTGCGC TGAGCTCGAG GCCATGGTTA GGCTACACCG ACCCACTTAC TAAGGGCTTC 
ATGATGACGC CCCTAAGTTA CACCGAGCTT GAGTATGGTT GGAACTTCAG TAGCGAACAG 
TGCTTGGAAG GCATGGTTGG TATTCATGCC AACTACCTAA GAATCTTTTC AATCGAAAAA 
CTGGGCGACA ACATGATTCA GAAATCCATT CCTTTGACTT ACACACCTAA GCACTTGGTC 
AAGCATCCCG AACAGCCATA CTTTTACACT ATCGAATCCG ACAATAACAC CCTCCCCCCG 
GAGCTGCGGG CAAAGCTTCT GGAGCAACAA AGCAATGGCG ACGCAACGGT ACTGCCGCCA 
GAGGACTTTG GATATCCCAG AGCCAAAGGT AGATGGGCAT CGTGCATCAG CATTATCGAC 
CCTATCAGTG AGGAACCGAG AGTCCTCCAA AGGATTGATC TTGACAACAA TGAGGCGGCA 
GTCAGCGCGG CCATTGTACC TTTTGCCAGT CAAGAGGGCG AAAGTTTCCT GGTTGTCGGC 
ACGGGCAAAG ATATGGTCCT CGACCCACGT CAATTTACCG AGGGCTACAT CCATGTTTAC 
AGATTCCATG AAGACGGCCG GGACCTGGAG TTCATTCATA AGACCCGAGT AGAAGAGCCG 
CCTTTGGCAC TCATTCCGTT CCAAGGACGT CTGCTTGCTG GCGTCGGCAA GACACTCCGT 
ATCTACGACC TCGGTTTGAA GCAACTGCTC CGAAAAGCTC AAGCAGATGT GACGCCCACT 
CTCATCGTCT CCCTACAAAG CCAGGGTAAC CGCATCATTG TCGGCGATCT CCAGCAAGGC 
ATCACGTACG TTGTCTACAA GGCCGAGGGC AATAGACTCA TTCCCTTTGC TGACGACACC 
TTGAACCGCT GGACAACCTG CACCACCATG GTCGACTACG AATCCGTTGC CGGCGGCGAC 
AAGTTCGGCA ACATATACAT CGTCCGTTGC CCAGAGCGCG TCAGCCAGGA AACTGACGAG 
CCTGGATCAG AGATCCACCT GATGCACGCC CGCAACTACC TTCATGGCAC ACCCAACCGC 
TTGAGCCTCC AGGTCCACTT CTACACTCAG GACTTGCCGA CCAGCATATG CAAGACCAGC 
CTAGTTGTCG GTGGTCAGGA CGTGCTGCTC TGGAGCGGCT TGCAGGGCAC TGTCGGTGTT 
TTCATTCCCT TTGTCAGCCG TGAGGATGTC GACTTCTTCC AGAATCTGGA GAATCATATG 
CGCGCTGAAG ACCCGCCGCT TGCCGGCCGT GATCATTTGA TCTACCGGGG TTACTATACC 
CCCGTCAAGG GTGTGATCGA TGGTGACTTG TGCGAGAGGT TTAGCTTGCT GCCGAATGAT 
AAGAAGCAGA TGATTGCGGG AGAACTTGAT CGCTCGGTGA GGGAAATCGA GCGCAAGATT 
TCGGATATCC GCACCCGGTC TGCCTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Nature422(6934)859-68(2003 Apr)
Galagan JE,Calvo SE,Borkovich KA,Selker EU,Read ND,Jaffe D,FitzHugh W,Ma LJ,Smirnov S,Purcell S,Rehman B,Elkins T,Engels R,Wang S,Nielsen CB,Butler J,Endrizzi M,Qui D,Ianakiev P,Bell-Pedersen D,Nelson MA,Werner-Washburne M,Selitrennikoff CP,Kinsey JA,Braun EL,Zelter A,Schulte U,Kothe GO,Jedd G,Mewes W,Staben C,Marcotte E,Greenberg D,Roy A,Foley K,Naylor J,Stange-Thomann N,Barrett R,Gnerre S,Kamal M,Kamvysselis M,Mauceli E,Bielke C,Rudd S,Frishman D,Krystofova S,Rasmussen C,Metzenberg RL,Perkins DD,Kroken S,Cogoni C,Macino G,Catcheside D,Li W,Pratt RJ,Osmani SA,DeSouza CP,Glass L,Orbach MJ,Berglund JA,Voelker R,Yarden O,Plamann M,Seiler S,Dunlap J,Radford A,Aramayo R,Natvig DO,Alex LA,Mannhaupt G,Ebbole DJ,Freitag M,Paulsen I,Sachs MS,Lander ES,Nusbaum C,Birren B

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NCU00396 cDNA ORF clone, Neurospora crassa OR74A

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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